LOCUS       DAOQYE010000001        27997 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_1972_length_27997_cov_5.050426, whole genome shotgun sequence.
ACCESSION   DAOQYE010000001 DAOQYE010000000
VERSION     DAOQYE010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 27997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27997
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1972_length_27997_cov_5.050426"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="PKN52_00005"
     CDS             complement(<1..407)
                     /locus_tag="PKN52_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98343.1"
                     /translation="MATGVTKIADIVVPEIFTPYVQQLTEEKAQIIQSGILSVDPALT
                     ELMAGGGLTFNAPSWKDLDNDADNVSSDNDGATSTPNKIGTLTEIAVRLSRNNSWSSM
                     DLAAALAGEDPMAAIANRVAYYWARRLQAATVAT"
     gene            complement(541..1359)
                     /locus_tag="PKN52_00010"
     CDS             complement(541..1359)
                     /locus_tag="PKN52_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98344.1"
                     /translation="MALSVIHDKIDDIPEPFRELYTEKDGKFHLTGIEGIKTQSDIDR
                     INEGLRKERDDHKKTRERFKAFDGLNADEVLAKLDRYDELEAAAAGKLDDDKINDIAE
                     KRVKSRLAPVERELLNIKKQNEELTGLVGQYQAKERTRTIHDAVRQAATKHKVLDTAQ
                     EDVLMLAERVFEVSEDGKVVAKDGVGITPGIDADLWLQEMQQKRPHWWAPSSGGGGTG
                     SGGGAGMANNPWSSDHWNLTKQGEYIRQHGTEKAAQMAKLAGVKLGATGPARKK"
     gene            complement(1457..1936)
                     /locus_tag="PKN52_00015"
     CDS             complement(1457..1936)
                     /locus_tag="PKN52_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98345.1"
                     /translation="MGALLAIGVCTAMVVTAPARFYEWLRENWRGAMLVSLLTALLVA
                     TTCSVAQAREYKVGDQTVVAAFCLVDEDPRPLIRILQENDDAAYFAHMTDLDKSCVDV
                     RIDPRLAGYGPIPVTVQYVGPTFETPLGCKQIIGFVDTEGQKGYTWTSADPLACQGI"
     gene            complement(1945..3144)
                     /locus_tag="PKN52_00020"
     CDS             complement(1945..3144)
                     /locus_tag="PKN52_00020"
                     /inference="COORDINATES: protein motif:HMM:NF016148.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HNQ98346.1"
                     /translation="MADVNEELFDALVRHQIYLMRLSGGIRRRIEDILNATEEDLAEK
                     IRDRLRNATGGLTPTNLRRMQVLRRIIANIRTRAWDQIDELWLSEMIDIAKAEPEFMA
                     GAMRTVAPVTLDLVIPPVATLRALVTSRPFEGRTLREWAQSIRRDDLRRIESAVQVGI
                     ASGESSAQIARRVVGTARLRGVDGMTEITRRAAAAITRTAVSHVSNAAKQEFYRENSD
                     VIQEELYVATLDARTTPVCRANDGKRFALGKGPMPPLHFNCRSLRVALFDGRALGTRP
                     AKPVTERQLVREFAQRRGLSGVTTRDSLPRGTKGAYDKFAQARIRELTGQVRADVSYQ
                     QWLKRQSAAFQDDILGKTKGALFRRGGLQLDKFVNRAGDELSLSDLARRHRDAFVAAG
                     LDPEDFI"
     gene            complement(3146..3367)
                     /locus_tag="PKN52_00025"
     CDS             complement(3146..3367)
                     /locus_tag="PKN52_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98347.1"
                     /translation="MSTENRVVEMPRSDHLRPEQALAEASKTDWDEVIVIGIDKSGAF
                     DMVNSEISLERALFLLEWARRYCWSSTEE"
     gene            complement(3357..3584)
                     /locus_tag="PKN52_00030"
     CDS             complement(3357..3584)
                     /locus_tag="PKN52_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98348.1"
                     /translation="MTHRERRHAIDQARAELAAEQLRTGDKTSRVVVCHGEPRCDKTW
                     PDWRPCEWCYHLDPLDKRTTERVLEDMAVEH"
     gene            complement(3565..5034)
                     /locus_tag="PKN52_00035"
     CDS             complement(3565..5034)
                     /locus_tag="PKN52_00035"
                     /inference="COORDINATES: protein motif:HMM:NF024661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4055 domain-containing protein"
                     /protein_id="HNQ98349.1"
                     /translation="MALNSVHPLYAKHHEDWCLMRDVYRGERAVKEKRRTYLPATSGM
                     IADGVDVKGRPGYEAYQAYLTRAVFHDFVAEAVEAMIGMMHHKPPAIELPAALEPLRE
                     LATLNGESLAQLLRRINEAQLVTGRIGLLLDLPAEASLAPRPYIATYKAEDILNWDDG
                     RRDELTLQTLNLVVLNESEFERQREFEWELKEKYRVLMLGRPDENEPSASGAVYRVGT
                     FRDANTTFSEAAMRTPSIRGRSLERIPFTFINSKDILPEPDGPPLLGLAKLALAIYRG
                     EADYRQTLFMQGQDTLVIVGGNAEDELRVGANAKINLPIGGDAKYIGVGHQGLAEQRQ
                     ALENDKRQAATKGAQMIDTMSREKESGEALKVRVSAKTATLNDVALAGAGGLQQLLRT
                     AAEWMGADPMQVVVTPNLDFSDDEMVAKDLVDLVSAKMMGAPISNRSIHLLMHKKGLT
                     EMEYEDELAEIEQEEPLVEPAGTGAGGDPEEDEGDTQGA"
     gene            complement(5089..6597)
                     /locus_tag="PKN52_00040"
     CDS             complement(5089..6597)
                     /locus_tag="PKN52_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98350.1"
                     /translation="MAALAPRADSHHRLPPGVRPVWWPQPGSQLFFLTCPLFECLFEG
                     TRGPGKTDALLMDFFQDVGKGYGPDWRGILFRQTYPQLQDVIAKTKKWFPQLCPGARY
                     NEALHQWVFPDGEMLLLRQFKRDDDYWNYHGHEYPWQGWEELCNWPTYVGYKRMMSTC
                     RSSNPRVAIIARVRATTNPYGPGHNWVKHRWRLPGSRYIPITDSLDEDGRLEPPRIAI
                     HGNIWENKILLEADPGYIDRIRASARNAAELRAWLYGSWDIVAGGMFDDVWDPKINVV
                     PTFVIPHTWRIDRSFDWGSSKPFSVGWWAESDGSDLMLPGGRVMSTVRGDLFRIHEWY
                     GWNGKPNEGLRMLATDIARGIVEREKRWGILGRVRPGPADTSIFDTENGVCIATDMEK
                     KVRLDDGKEYRGVSWTRADKRPGSRKTGWEAMRKMLAQAKRPKTGPRELPGLFITDRC
                     QHWINIVPVLPRDEKDTDDLNTEAEDHIADETRYRVRTVGARAEGGRTSGMY"
     gene            complement(6572..7159)
                     /locus_tag="PKN52_00045"
     CDS             complement(6572..7159)
                     /locus_tag="PKN52_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98351.1"
                     /translation="MANKKDGTPRASTTGGPGPRVVDMSNWRRRLQESRLKFDDRAKN
                     IFLEKYAECGLMYVSARAAGVTPPTVTSHVENDPDFAAAFQDAKDAYRDRVMAHAYKL
                     SIEGVEEPIIGGKDKDEVVAYKKVYATNILAMEMRRVNPDYKDRADVNLSGNVGGVLV
                     VPARLSVEEWTKAAAEYRDQMPGEGKDGGSSGTKG"
     gene            complement(7588..8025)
                     /locus_tag="PKN52_00050"
     CDS             complement(7588..8025)
                     /locus_tag="PKN52_00050"
                     /inference="COORDINATES: protein motif:HMM:NF019128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HNQ98352.1"
                     /translation="MVRLAAGALSLLSSSAVLSQEPPLRADEHLVRMVLQEAEGEPLD
                     GMIAVAGVALDRARDRRWPDTVREVVYQPAQFQGLSLPDRAWTERAIRRARRAVRRAR
                     AGERPCGRVLWYHTVDVRPRWVEELTPVCVIGYHVFWNDPSRL"
     gene            complement(8040..8681)
                     /locus_tag="PKN52_00055"
     CDS             complement(8040..8681)
                     /locus_tag="PKN52_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98353.1"
                     /translation="MIIVPEIQTVVLLVPRTGSGSLRRAVLGKYPQAYQLYRHMEADG
                     VPVGYDRWRRVGVVRHPIDRLFSMYKFCRDGMGGGYPEYAARMRRSTEGGFEHWLLHN
                     ETVFGGMFDSEDGQRSWAQFAIRHALPENRKSQWHYLRPDLGTVCYPFHWMWLLYADL
                     GLGAPHLRENATEREPWPELSGAGLDHIRRHHGWDLEHWDAMPVTRTLVGSAA"
     gene            complement(8665..8985)
                     /locus_tag="PKN52_00060"
     CDS             complement(8665..8985)
                     /locus_tag="PKN52_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98354.1"
                     /translation="MTDNRPRCRERHVPDGGRWPTFHQCTRLAVRDGYCKQHHPEAVR
                     ERRQRSHDQSEARWQERRLEIYGKSAIGLLREIAKGHNDPCNAAREWLRENKFEESGD
                     DHRT"
     gene            complement(9010..10284)
                     /locus_tag="PKN52_00065"
     CDS             complement(9010..10284)
                     /locus_tag="PKN52_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98355.1"
                     /translation="MTEDIRTFEIPVVRWDDFVAKFAKLNRRAAKLGCEPCTFAVVGE
                     AVVKRTVVYKYEGEEHSREHDVPVRLVTVAGAAPRLADWALVARVEELAEGLILIHSV
                     PGVEGRVDPRFRSLGAHVCEHCRKARRRRDVFVVRHADGRELQVGRQCLADFTGINSP
                     ERAAAQATWLCMVSDLVEDGGWFSGLGADHVPVLEALELTSAYIRAYGWQSRSAANNG
                     GDRATADLVADHFAWTSVVRSEAKTRLVKLGGLTDDDTRRAGETIRWVEQDLAAKEGR
                     SDYEENLVQLVLHDLASTRHLGIVCSALATALRVKSAWELRKAKEAELLKSVHVGAQG
                     ERLRGVQARVLSVRSKGGDFGASTLVKLVTADGNVLTWWASGDVDVEPGAEVTLDGTV
                     KRHGEYNGVKETQLTRVKAEGLEIHRSSRRYW"
     gene            complement(10288..10518)
                     /locus_tag="PKN52_00070"
     CDS             complement(10288..10518)
                     /locus_tag="PKN52_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98356.1"
                     /translation="MNATTTRETPRLLYVLRRQDARYGVYAVTATDARSAVALPCRTL
                     RGAERKLRNLARGYGMRVERVENGELRAWMEG"
     gene            complement(10610..10921)
                     /locus_tag="PKN52_00075"
     CDS             complement(10610..10921)
                     /locus_tag="PKN52_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98357.1"
                     /translation="MSRERAQEALRWSPLGSRPGSLYALPQAERRDLLEALWAELGLT
                     ARLTRGRPAYATRPGTHTLVRTLDGWWNVLGLERLAEEDHRLVVAVLTDLRHGEYTIY
                     G"
     gene            complement(11100..11309)
                     /locus_tag="PKN52_00080"
     CDS             complement(11100..11309)
                     /locus_tag="PKN52_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98358.1"
                     /translation="MTLSLAYLRRSLRACTDCAERQLGLRYVREAQALGGRNPEREAE
                     LMGQLPRLLAYAEERTRRAGREIRR"
     gene            complement(11327..11512)
                     /locus_tag="PKN52_00085"
     CDS             complement(11327..11512)
                     /locus_tag="PKN52_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98359.1"
                     /translation="MSATKTIAASLDWCRQIGRAMAADGRTPEEVDQFLRSIGRQPEG
                     VTYRRVQRAYRDQLARA"
     gene            complement(11650..11859)
                     /locus_tag="PKN52_00090"
     CDS             complement(11650..11859)
                     /locus_tag="PKN52_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98360.1"
                     /translation="MAKTPITRLWLQTTATGERVCARLADGTVRVLTPSLGQFSAPIE
                     LERRARRAGLKVRETQAGPVAERED"
     gene            complement(12027..12308)
                     /locus_tag="PKN52_00095"
     CDS             complement(12027..12308)
                     /locus_tag="PKN52_00095"
                     /inference="COORDINATES: protein motif:HMM:NF019884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HNQ98361.1"
                     /translation="MPTKSKIDGLSETQRKVASLLARQRGATVSDLAEACSMSERAIR
                     NLIDRLRGKGLRIENVQRGSGRFHLARSAASGRTRRRGRRAVREASAEA"
     gene            complement(12422..12622)
                     /locus_tag="PKN52_00100"
     CDS             complement(12422..12622)
                     /locus_tag="PKN52_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98362.1"
                     /translation="MTRTELVRALAPLLGPARREALGRLARGEADEIAIRYDEVRDWA
                     ALGRVLSQVPYWDGEEWLTLSN"
     gene            complement(12619..12861)
                     /locus_tag="PKN52_00105"
     CDS             complement(12619..12861)
                     /locus_tag="PKN52_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98363.1"
                     /translation="MIKVLILVCNLALHPAHSTCQSGAEGVETYPGPAADSLVQCGLY
                     GQAYLAEQHIGRRLDLSEEYVKVRCSWGGPAAGNVG"
     gene            complement(12858..13079)
                     /locus_tag="PKN52_00110"
     CDS             complement(12858..13079)
                     /locus_tag="PKN52_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98364.1"
                     /translation="MSPLEEMLLRRRQSARRHTDGLGPLMVPCLVDGREVLGECLPPG
                     PAPSHVILHPDGRVEPVTPKRGSIVDIDA"
     gene            complement(13076..13444)
                     /locus_tag="PKN52_00115"
     CDS             complement(13076..13444)
                     /locus_tag="PKN52_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98365.1"
                     /translation="MRMPGKVRVGHQTLPIIREDIAETDNAVGMYHDGTERSPCIVLD
                     RRLRGQEVARVLLHELTHAILNCYMPVETYKAVFEKRRLPRQDVEEMVADAMGKGLMQ
                     VVRDNPALWRWLQGHIGEHR"
     gene            complement(13441..13860)
                     /locus_tag="PKN52_00120"
     CDS             complement(13441..13860)
                     /locus_tag="PKN52_00120"
                     /inference="COORDINATES: protein motif:HMM:NF039184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5664 domain-containing protein"
                     /protein_id="HNQ98366.1"
                     /translation="MSKRKRASSSNPKDIAALDRVPMHLLPAAGAIYGAMACGDGAEK
                     YGPYNWRDKPISLMSYLGAIERHIACIKDGEDAATDSLIDHLGHINATTAILLDARQV
                     GTLIDDRPRVRGRASALLEGYRDRRRAARERKRRPAS"
     gene            complement(13964..14467)
                     /locus_tag="PKN52_00125"
     CDS             complement(13964..14467)
                     /locus_tag="PKN52_00125"
                     /inference="COORDINATES: protein motif:HMM:NF013654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase
                     family protein"
                     /protein_id="HNQ98367.1"
                     /translation="MSKGCATLDAVEEFHRLFEIPVEDRPTLPGWDEDMSDRLQRYAD
                     SMAELAKDLHAEARIHGPRAGLLLLRLQLLQEELAELAQGMASGDLRGCLDALADLRY
                     VQDGTTLSLGLGTVFMDAFREVHRSNMSKLEHGRPVKNEAGRVVKGRCYTPPDLQPIL
                     EAVQQGV"
     gene            complement(14464..14661)
                     /locus_tag="PKN52_00130"
     CDS             complement(14464..14661)
                     /locus_tag="PKN52_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98368.1"
                     /translation="MSIELVIVYLTLGILIAELCSWNERRSGREPGAGAYMWLSLVWP
                     AVLAMALVAIAARPQAEEDEE"
     gene            complement(14679..14879)
                     /locus_tag="PKN52_00135"
     CDS             complement(14679..14879)
                     /locus_tag="PKN52_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98369.1"
                     /translation="MTYDHLMQADLGDLVDLTLDPATPEVERLQAREALCARLRGEQW
                     DWLQFTEEQRAQIKTICGDCGV"
     gene            complement(14923..15204)
                     /locus_tag="PKN52_00140"
     CDS             complement(14923..15204)
                     /locus_tag="PKN52_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98370.1"
                     /translation="MSAGTILGRGVLLIALAASATATAEDLIIRDESGRRVGTIESSP
                     INPDNLIIRDERGRRVGTIEPDPVNPGRLIVRDQSGRRTGTIEPPFTVH"
     gene            complement(15274..15876)
                     /locus_tag="PKN52_00145"
     CDS             complement(15274..15876)
                     /locus_tag="PKN52_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98371.1"
                     /translation="MGLAYIAHDSEGIRLEFTERWDILYLQQHGPYSMEFDGCNLMVS
                     EDPSGMLYACVIHQEHGRNGLRRVRHATQPAFLGRFGPQEIDVDLGLGGGFIAELPPI
                     YERPWPVLTIGCPRYNYEAQLLVTLAERLRAFRDDHGVIDLSELLSETRIPRSVKRRA
                     DRDREFRADLLRLVKDGSRLRRLQWGVHPLRDDQWERPIA"
     gene            complement(15876..16454)
                     /locus_tag="PKN52_00150"
     CDS             complement(15876..16454)
                     /locus_tag="PKN52_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98372.1"
                     /translation="MTKSHDTREGWLRDALGQLDATFFRERGYTLPEKIQVSCGFPKG
                     HATAIGQCFDPEVAADGAVHIFICPTQAEPIRILDILLHELIHAAVGIKEGHKGLFRK
                     LAKEFGLAGKMTSTYAEAGSELHKTLEVMAGRLGAYPHAPMRKPRKQKGGSGWIRLKS
                     PENERYTVVISPKALDEHGFPMDPWGNEMEPV"
     gene            complement(16451..16690)
                     /locus_tag="PKN52_00155"
     CDS             complement(16451..16690)
                     /locus_tag="PKN52_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98373.1"
                     /translation="MSMRVGPCMHEAVLIVRANPGTAILPVARAIGPHGSLMYGYNAV
                     HRAINAGLIDAQRHGGRYSLTVSEKGVAFLQGREA"
     gene            complement(16709..16966)
                     /locus_tag="PKN52_00160"
     CDS             complement(16709..16966)
                     /locus_tag="PKN52_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98374.1"
                     /translation="MINKSASRVGTLVFKSGIRNTLYVGTNSNTGETILWQQCGDRRA
                     KRSTGRNLRRVVKELSYVLASGEARWDGEVNGASLAALQQA"
     gene            complement(16979..17383)
                     /locus_tag="PKN52_00165"
     CDS             complement(16979..17383)
                     /locus_tag="PKN52_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98375.1"
                     /translation="MSTSKTNLGRLSLLRRHLEDQAETAWEQLAEFQEKLSQNPFFAL
                     EWAGSAYQAAAELQLAQEILHAMGPDNKLNVARTEEGVGKFLDHVVKHLTEDCLRAAR
                     HPERSTSPWSNEMARELASAKARLLERLTGAW"
     gene            complement(17529..19925)
                     /locus_tag="PKN52_00170"
     CDS             complement(17529..19925)
                     /locus_tag="PKN52_00170"
                     /inference="COORDINATES: protein motif:HMM:NF040238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5906 domain-containing protein"
                     /protein_id="HNQ98376.1"
                     /translation="MAPVKPDNKAALAFLQRWAPEGPWVLTAIQADRKAIATATFRPA
                     SLHQLEQWLEQYNGHRNIYFHVNPVLRDVTKKAEREDIRAVAWLHVDIDPRAGEDLQA
                     ERERALALLTTRLPQGVPEPTCVVFSGGGYQGFWRLEQPIQVDGDLAKAEDAKRYNQQ
                     LEILFAADNCHNIDRIMRLPGTVNIPDARKLKKGRQPALAELVSFNDHVYPLSRFTPA
                     PVVQMPEERGFGGGATVKVSGNVERLAGIEELDQWGVPDRVKVIIVQGKHPDEVKEGD
                     NSRSAWLFDAICQLVRAGVPDDIIFSVVTDPEFSISESVLDKGANAEKYAIRQIERAK
                     EEVEDPWLRKLNEQFAVIGNIGGKCRVIEEVLDPALRRTRLTRQSFDDFRNRFMHQRT
                     QVGDDPKTGLPIMMQVGKWWLQHQHRRQYETIVFAPGHEVKGAYNMWKGFACQSRPGD
                     CSLFIAHIRENICQGDETLFIYVMGWLARLVQHPDTPGEVAIVLRGGRGVGKSFFAKI
                     VGHLLGRHFLQVSNPSHLIGNFNAHLRDVVLLFADEAFYAGDKRHSSILKTLITEETI
                     TIEAKGVDAEASPNYVHLIMASNDMHVVPAGGDERRFLVLDVGKGHQQDAGYFGEIIR
                     QMESGGYEALLHMLLTYDLSGYEVRDVPRTEALLEQKLLSLSLEEEWWYNKLLEGRLL
                     LEDRWQREVVIKDLFDDYIAYTRKLNTNRRSGQTALGRFLHRVVPGLGHTQRIADRDI
                     QTGEGWTRTVRERTRFYTIDSLEGCRRRWEELYGRQEWPEAPGAQGELPQPRGGGTPF
                     "
     gene            complement(20025..20360)
                     /locus_tag="PKN52_00175"
     CDS             complement(20025..20360)
                     /locus_tag="PKN52_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98377.1"
                     /translation="MSGRKTELGELTRGERLLIDRRRRGEGQIAAARRLGLTHSMYGK
                     MERDAVDWGMPVEIGELAAHERCLLYRRRAGFTQARVAEELEVCRFWVNQMEQGLVGC
                     DTLLWYWEQ"
     gene            complement(20327..20629)
                     /locus_tag="PKN52_00180"
     CDS             complement(20327..20629)
                     /locus_tag="PKN52_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98378.1"
                     /translation="MELKWLRRWPENADTDPVLIRHFTQQQRVWLSRRCRRGGLAWLL
                     LQVGQEYLLFDGETAAIIVGRAPRGALLRGASRYWPRGLRHSELVECLAGRQSSAS"
     gene            complement(20739..21179)
                     /locus_tag="PKN52_00185"
     CDS             complement(20739..21179)
                     /locus_tag="PKN52_00185"
                     /inference="COORDINATES: protein motif:HMM:NF012601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deaminase"
                     /protein_id="HNQ98379.1"
                     /translation="MSSQDKWDIRFLQLAEIVAQWSRDPSTKCGAVIVRPDRTVASLG
                     FNGFPRGMYDAEALYHDRDEKYSRVVHAEINAVLHAHGPVAGCTLYTWPFCCCDRCAV
                     QMIQAGITRVVSPSLPADKAERWERVLIRSLRYFQECGVEVTLV"
     gene            complement(21176..23155)
                     /locus_tag="PKN52_00190"
     CDS             complement(21176..23155)
                     /locus_tag="PKN52_00190"
                     /inference="COORDINATES: protein motif:HMM:NF012688.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HNQ98380.1"
                     /translation="MAKRNSNRPGLLQLPLFTPESNWSPPDLSSLPSWAEAKRVCVDV
                     ETYDPHLRQLGIGVRRGGYVVGVSFKIEDGPGAYLPMRHQGGDNLPVEQVLGYLRENA
                     ARFSGQLVGANLPYDLDYLWEEGVYFPQVELYRDVQIAEPLIDELQMSYSLDAIAKRH
                     GLPGKSEELLEQAARAYGVATKHGMWQLPARYVGPYAEVDTEQPLLTLRRQERIIDER
                     DLWRIYDLESRVLPVLVRMRRRGVRVNMERLRGIETWSLDKETEALQLVRDQTGVQIA
                     VGDVWKADALAPALHYIGVKLHQTSTGKPSIDKDLLSSIDHPVAKALAWARKVNKLRT
                     TFAASVRTYMVNGRIHCTYNQMAREDDKGDQKGARYGRVSCVDPNLQQQPSRDEFAKM
                     WRSIYEPEEGALWAACDYSQQEPRWTTHFAAMMDLPGAREAAQAYHDNPKLDNHQFMA
                     DLTGLDRKYAKNIYLGLCYGEGGAKLCDDLGLPTRWALAMGRGRERRVAYFSTQAEAL
                     EARAQEGGEGFVWRAAGEEGQRILDVFDARAPFIRKLARKAEDVAKARGYVVTIGGRH
                     LHFPARGDGSYEWTHKALNRVIQGSSADQTKTAMVELDRMGHFLQLQVHDELDSSVRD
                     REEAMRMAEVMSNCIPAEVPFRVDVEIGPSWGEAE"
     gene            complement(23155..23490)
                     /locus_tag="PKN52_00195"
     CDS             complement(23155..23490)
                     /locus_tag="PKN52_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98381.1"
                     /translation="MEVHYLDLVACRNDWSVIGWDFPRFLRELEFRFPLAGHVARRNG
                     KTLGRDGLYNRVQCSYYGEGDGRHHLSNRHPAAGEGGAALMRLIRDELATVGYISEAA
                     FLRLMNSID"
     gene            complement(23643..23900)
                     /locus_tag="PKN52_00200"
     CDS             complement(23643..23900)
                     /locus_tag="PKN52_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98382.1"
                     /translation="MRGPEQPEPLDPVHEAAGKVVEALSEYDLQVALTVLTNVCGQVV
                     AALAEGRPSEVQRQGTSLAENIKRAAIAKLLHDDEQRRSGT"
     gene            complement(23900..24526)
                     /locus_tag="PKN52_00205"
     CDS             complement(23900..24526)
                     /locus_tag="PKN52_00205"
                     /inference="COORDINATES: protein motif:HMM:NF023181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3310 domain-containing protein"
                     /protein_id="HNQ98383.1"
                     /translation="MSHDRGCGCGLERWEYPECPRGRDCIKWVDEPEDRVRPSDRTAL
                     EKLVSMAEKDRYGHEDPNKRQVGGGHYKTDMEHWDLIENAGLGYLEGCATKYVTRWRK
                     KAGLQDLEKAMHYVDKLLALSSRPVAPRKNRGQAAVEDLLRYAVANELTARETLIMCL
                     LCQWVSPSDLERARDEIAEMIRLEREKGETKTSGQDHPFGYFADEEIR"
     gene            complement(24523..25083)
                     /locus_tag="PKN52_00210"
     CDS             complement(24523..25083)
                     /locus_tag="PKN52_00210"
                     /inference="COORDINATES: protein motif:HMM:NF024319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNQ98384.1"
                     /translation="MQPSKMHGIVNNLVQSRCGGRVERCHAIPHATSYSNAAHSWGVA
                     MLMYYLWPADFPRLAAVCLSHDLPEAWVGDVPAPTMNFVPGLRQQLGRIEDNLSERMG
                     LPRLDALPKDDLEKLKTCDRLELYLWCREQMLMGNRFAEETLKELEYYFTNLIQLPAP
                     ADEFLAYIQGSELLPVQAGVMREACK"
     gene            complement(25070..26107)
                     /locus_tag="PKN52_00215"
     CDS             complement(25070..26107)
                     /locus_tag="PKN52_00215"
                     /inference="COORDINATES: protein motif:HMM:NF012524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HNQ98385.1"
                     /translation="MQVITVRNVHEALPTALDLLSRLGEGRNSRNGPVRVFPFPVTTE
                     YHEPRERVIFWRDRDANPFFHLMESLWMLAGRNDVAFVAQYVKRMRSFSDNGKTFHAA
                     YGHRWRQHFGQDQLAEIVARLQDNPDDRRCVLQMWDCEVDLGKEGRDFPCNTQVYFSR
                     NSSSALDMTVCCRSNDLIWGAYGANAVHFSVLQEFMAAAIGCPVGTYWQMSNNLHAYE
                     DQLKRVAHLADEAADPFRLSGRNPYASGQVSWFPLVQTPILEWQQDLLMFMDHGPIVG
                     LRDPFFRRVVTPMYCAHKALQDKADPDRFDKALEILQQCRATDWQAASIGWVIRRRDE
                     AIAKEYHDAAE"
     gene            complement(26111..26713)
                     /locus_tag="PKN52_00220"
     CDS             complement(26111..26713)
                     /locus_tag="PKN52_00220"
                     /inference="COORDINATES: protein motif:HMM:NF037896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nmad5 family putative nucleotide modification
                     protein"
                     /protein_id="HNQ98386.1"
                     /translation="MASVKLTTALREIIRKRMLRHRFKETYDQLIQDRAALAKEVYTD
                     CFKDIMKQMAAVPAGWLPKDDVIKVQLAGKVHELPFHGQLYSNVTAFLRDSIPQTELT
                     LPYDKYKRVVKVYDARHPIAERHDELENRLTDLKRGINEATAGIDAALNAVTTVGKLL
                     EVWPEAAPFCGDLTQPAVQLPAIQTTVLNRMLGLPVQEAA"
     gene            complement(26715..27581)
                     /locus_tag="PKN52_00225"
     CDS             complement(26715..27581)
                     /locus_tag="PKN52_00225"
                     /inference="COORDINATES: protein motif:HMM:NF037498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA base hypermodification protein"
                     /protein_id="HNQ98387.1"
                     /translation="MHGGERFFAYARERYRVMLRRETGQPAPWTDDPILRQYRFCNVF
                     REDDRVTRWIAENVRRPLRRDPRVLLAMYIARWFNRIPTLERLEGRAVSNTNLLLTWN
                     RDYAERRLRDVKPLVTGAYMVKTPAGKNKLEGVLECIEAFVPHAERVGHEMLAARSLQ
                     HATELLQQFPYMGPFMAYEVVTDLRHTYLLDQATDILSWANPGPGAARGLGRVLYGEP
                     ERFDRHVKCQVDEMQEAMQMLLARSRNPLIWPSGWPKWEMREVEHTLCEFDKYERARL
                     GEGTPKQRYNGG"
     gene            complement(27584..>27997)
                     /locus_tag="PKN52_00230"
     CDS             complement(27584..>27997)
                     /locus_tag="PKN52_00230"
                     /inference="COORDINATES: protein motif:HMM:NF018154.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HNQ98388.1"
                     /translation="VIGHYETTCGGCDTISKMEHIFELARKAHDNGCDVVFEGLLISA
                     DVNRTAALWAFAQEQGCPMLVVALDVPLQVCLDSVNARRRERNPDLPPVNPRNTESKH
                     KGVQQSMRRLEAAGVPAVSLDRDAALARIRAELGL"
BASE COUNT         4128 a         9630 c         9138 g         5101 t
ORIGIN      
        1 gtggcaacgg tggcagcctg gagccggcga gcccagtagt aggcgacgcg gttggcgatc
       61 gcggccatcg ggtcctcacc ggcgagggcc gccgcgaggt ccatgctcga ccaggagttg
      121 ttgcggctca ggcggaccgc gatctcggtg agcgtgccga tcttgttcgg ggtcgaggtc
      181 gcgccgtcgt tgtcgctcga cacgttgtca gcgtcgttgt cgaggtcctt ccacgacggg
      241 gcgttgaagg tcagaccgcc accggccatc agctcggtga gggcgggatc cacggacagg
      301 atgccgctct ggatgatctg cgccttctcc tccgtcagct gctggacgta tggggtgaag
      361 atctccggga cgacgatgtc cgcgatcttg gtaacaccag tggccatggt ctagtctccc
      421 ttggctctct caagtttgaa gcggcaaacc tgagcatgcc ccatggccag ctcagagtgt
      481 caggaggaga gatggtgtct ctcctcctgc gaatatactc gggtgtctct cccgaggcaa
      541 ctacttcttg cgggccggcc ccgtcgcacc cagcttgaca ccggccagct tggccatctg
      601 cgccgccttc tcagtgccgt gctgacggat gtactcgccc tgcttggtca ggttccagtg
      661 gtcggacgac cacgggttgt tggccatgcc cgccccgcca ccgctgccgg tgccgccgcc
      721 accagaggac ggagcccacc agtgcggccg cttctgctgc atctcctgga gccacaggtc
      781 cgcgtcgatg ccaggcgtga tgccgacgcc gtccttggcg acgaccttgc cgtcctcgct
      841 gacctcgaac acccgctcgg ccagcatcag cacgtcctcc tgggcagtgt ccaggacctt
      901 gtgcttggtc gcggcctgac ggaccgcgtc gtggatggtg cgggtgcgct ccttggcctg
      961 gtactggccg acgagaccgg tgagctcctc gttctgcttc ttgatgttga gcagctcgcg
     1021 ctcgaccggc gccagccggc tcttcacacg cttctcagcg atgtcgttga tcttgtcgtc
     1081 gtccagcttg ccagcggcag ccgcctccag ctcgtcgtac ctgtcgagct tggccaggac
     1141 ctcgtcggcg ttgaggccgt cgaacgcctt gaaccgctcg cgggtcttct tgtggtcgtc
     1201 gcgctccttg cggagaccct cgttgatgcg gtcgatgtcg gactgcgtct tgatcccctc
     1261 gatgccggtc aggtggaact taccatcctt ctcggtgtac agctcgcgga acggctcggg
     1321 gatgtcgtcg atcttgtcgt ggatgacaga gagggccatg cctcttgctc cttgcgctca
     1381 ggttcacagc tgcccatgca gccgccccgt cccatgacga ggcggcgatg atgcactcct
     1441 cagagacacc aggctgtcat attccctggc acgccaatgg atcggcgctc gtccaggtgt
     1501 agcccttctg gccctcggtg tccacaaagc cgatgatctg cttgcagccc aggggtgtct
     1561 cgaacgtcgg gccgacatac tgcactgtca ccggtatcgg gccgtagccg gcaagtcgcg
     1621 ggtcgatgcg cacatcgacg cagctcttgt cgaggtctgt catgtgggcg aagtaggccg
     1681 cgtcgtcgtt ctcctggagg atgcggatga gcggacgagg gtcctcgtcg acaaggcaga
     1741 acgcggccac caccgtctga tcgccgacct tgtactccct ggcctgtgcc acgctgcacg
     1801 tggtggccac gaggagggcc gtgagcagcg acaccagcat cgcgccgcgc caattctcgc
     1861 gcagccactc gtagaaccgc gctggggcag tgacgaccat ggccgtgcag acgccgatcg
     1921 ctagcagtgc tcccataact ctcctcatat gaagtcctcc gggtctaggc cggccgccac
     1981 gaaggcgtcc ctgtgcctcc tggcgagatc gctgagcgag agctcatcgc cggctcggtt
     2041 cacaaacttg tcgagctgaa gtccgccgcg acggaacagc gcacccttgg tcttgccgag
     2101 tatgtcgtct tggaacgccg cagactgtcg ctttagccac tgctgatagc tcacgtcggc
     2161 ccgaacctga cccgtgagct ccctgatgcg cgcctgggcg aacttgtcat aggcaccctt
     2221 ggtgcccctg ggcagactgt ccctcgtcgt gacaccactc aggccccgcc tctgggcgaa
     2281 ctctcgcacc agctggcgct ccgtcaccgg cttggccggc cgggtgccca gggcacgtcc
     2341 atcaaacaga gccacacgga gggagcgaca gttgaagtgg agcggcggca tcggcccctt
     2401 gcccagggca aaccgcttgc cgtcgttggc gcggcacacg ggcgtcgtcc tcgcgtcgag
     2461 ggtcgccacg tacagctcct cctggatgac gtcgctgttc tcgcggtaga actcctgctt
     2521 ggccgcgttg gagacgtggc tgacagccgt gcgggtgatg gccgccgccg cgcggcgagt
     2581 gatctccgtc atgccgtcga cgcctcggag gcgggcggtc ccgacgaccc ggcgggcgat
     2641 ctgcgcgctg ctctcgccgg aggcgatgcc gacctgcact gcgctctcga tccggcggag
     2701 gtcgtcgcgg cggatggact gcgcccactc gcggagggtg cggccctcga agggacggct
     2761 cgtcaccagc gcgcgcagcg tggcgacggg cgggatcacc aggtccagcg tgaccggggc
     2821 gaccgtccgc atcgcgccgg ccatgaactc cggctcggcc ttggcgatgt cgatcatctc
     2881 gctcagccac agctcgtcga tctggtccca ggcccgagtg cggatgttgg cgatgatgcg
     2941 ccgcaggacc tgcatccgcc tgaggttggt cggggtgagc ccgccagtgg cgttgcgcag
     3001 ccggtcacgg atcttctcgg cgaggtcctc ctcggtggcg ttgaggatgt cctcgatccg
     3061 gcgccggatg ccgccgctga gccgcatcag gtagatctga tgccgcacca gggcgtcgaa
     3121 cagctcctcg ttgacgtcgg ccacgtcact cctccgttga ggaccagcag tagcgtcgtg
     3181 cccactccag caggaacaga gcccgctcca gagagatctc gctgttgacc atgtcgaagg
     3241 caccgctctt gtcgatgccg atgacgatga cctcgtccca gtcggtcttg gatgcctcgg
     3301 ccagggcctg ctctggcctc aggtggtcgg agcgtggcat ctctaccact ctgttctcag
     3361 tgctcaacgg ccatgtcctc caggaccctc tcggtcgtcc tcttgtcaag cgggtccagg
     3421 tggtagcacc actcgcaggg cctccagtcc ggccacgtct tgtcgcatcg cggctcgccg
     3481 tggcacacca ccacgcggct ggtcttgtcg ccggtgcgga gctgctcagc ggccagctcc
     3541 gccctggcct ggtctatcgc gtgcctacgc tccctgtgtg tcaccctcgt cctcttccgg
     3601 gtcgccgccg gcccctgtgc cggccggctc gacgagtggc tcctcctgct cgatctcggc
     3661 cagctcgtcc tcgtactcca tctcggtcag gcccttcttg tgcatcagca ggtggatcga
     3721 gcggttgctg atcggggcgc ccatcatctt ggcgctgacc aggtccacca ggtccttcgc
     3781 caccatctcg tcgtcgctga agtcgaggtt cggggtgacg actacctgca tcgggtcggc
     3841 gcccatccac tcggcagcgg tgcgcagcag ctgctgcagc ccgcccgccc ctgccagcgc
     3901 gacgtcgttc agggtggcgg tcttcgccga tactcgcacc ttcagggcct cgccgctctc
     3961 cttctcgcgg ctcatcgtgt cgatcatctg cgcgcccttg gtcgcagcct gccgcttgtc
     4021 gttctccagt gcctgccgct gctcggccag accctggtgg ccgacgccga tgtacttggc
     4081 gtcaccgccg atcggcaggt tgatctttgc gttcgcgccg acgcgcagct cgtcctccgc
     4141 gttgccaccg acgatgacca gcgtgtcctg accctgcatg aacagggtct gccggtagtc
     4201 ggcctcgccc cggtagatgg ccagggccag cttcgcgagg cccaggagcg gcggcccgtc
     4261 gggctccggc aggatgtcct tgctgttgat gaacgtgaac gggatccgct ccagcgagcg
     4321 gccccggatg ctgggcgttc tcatcgccgc ctcggagaac gtcgtgttgg cgtcgcggaa
     4381 cgtgccgacc cggtagaccg cgcccgaggc cgacggctcg ttctcgtccg gccggccgag
     4441 catcagcacc cggtacttct ccttgagctc ccactcgaac tccctctgcc gctcgaactc
     4501 actctcgttg agcaccacca ggttgagggt ctgcagcgtc agctcgtcgc ggcgaccgtc
     4561 gtcccagttg agaatgtcct cggccttgta cgtggcgatg tagggacgtg gggccagcga
     4621 ggcctcggcg ggaaggtcca gcagcaggcc gatgcggccg gtcaccagct gcgcctcgtt
     4681 gatgcgccgc agcagctggg ccaggctctc cccgttcagg gtggccagct cgcgcagcgg
     4741 ctccagggcg gcgggcagct cgatggcggg cggcttgtgg tgcatcatgc caatcatggc
     4801 ctcgacggcc tcggcgacga agtcgtggaa gacggcccgc gtcaggtagg cctggtacgc
     4861 ctcgtacccg ggacgaccct tgacatccac gccgtcggcg atcatgccgc tggtcgcagg
     4921 aaggtacgtg cgacgcttct ccttgacggc ccgctctccc cggtacacgt cgcgcatgag
     4981 gcaccagtcc tcgtggtgct tggcgtacag cgggtggaca gagttgaggg ccatggtcgt
     5041 ctctccagtt gtctcgcgca gtctgctccc gagggagagc ggccgcaatc aatacatgcc
     5101 cgaggtccgg ccgccctccg cccgcgcgcc taccgtgcgg acgcggtagc gggtctcgtc
     5161 tgcgatgtgg tcctcggcct cggtgttgag gtcgtcggtg tccttctcat cgcggggcag
     5221 caccgggacg atgttgatcc agtgctgaca gcggtcggtg atgaacagcc cagggagctc
     5281 gcgcgggccg gtcttgggcc tcttggcctg ggccagcatc ttgcgcatcg cctcccatcc
     5341 ggtcttccgc gagccgggtc gcttgtcagc gcgcgtccag ctcacgcccc tgtactcctt
     5401 gccgtcgtcg aggcggacct tcttctccat gtccgtcgcg atgcacacgc cgttctcagt
     5461 gtcgaagatg gacgtgtcgg ccggcccagg gcggacgcgg ccgaggatgc cccaccgctt
     5521 ctcccgctcg acgatgcccc tggcgatgtc cgtcgccagc atgcgcaggc cctcgttcgg
     5581 cttgccgttc cacccgtacc actcgtggat gcggaagagg tcgccgcgca ccgtcgacat
     5641 gacccggccg ccgggcagca tcaggtccga gccgtcgctc tcggcccacc accccacgct
     5701 gaacggcttg ctgctgcccc agtcgaagct gcggtcgatg cgccacgtgt gcgggatgac
     5761 gaaggtcggc acgacgttga tcttcgggtc ccacacgtcg tcgaacatgc cgccggccac
     5821 gatgtcccag ctgccgtaca gccacgccct cagctcggcc gcgttgcggg ccgacgcgcg
     5881 gatgcggtcg atgtagccgg ggtcggcctc caggaggatc ttgttctccc agatgttgcc
     5941 gtggatggcg atgcgcggcg gctcgaggcg accgtcctcg tccaggctgt cagtgatcgg
     6001 gatgtagcgg gagccgggca gccgccagcg gtgcttgacc cagttgtgcc ccggcccgta
     6061 ggggttggtg gtggcgcgca cgcgggcgat gatggccacg cggggattgc tcgaccggca
     6121 cgtggacatc atgcgcttgt agccgacgta ggtcggccag ttgcacagct cctcccaccc
     6181 ctgccacggg tactcgtgac cgtggtagtt ccagtagtcg tcgtctcgct tgaactgccg
     6241 gagcagcagc atctccccgt ccgggaagac ccactggtgc agggcctcgt tgtatcgcgc
     6301 gccagggcac agctgcggga accacttctt ggtcttcgcg atgacgtcct gcagctgggg
     6361 ataggtctgc cggaacagga tgccgcgcca gtcaggcccg tatcccttgc cgacgtcctg
     6421 gaagaagtcc atcagcaggg cgtcggtctt tcccgggcct cgtgtcccct cgaacaggca
     6481 ctcgaagagc gggcaggtca ggaagaacag ctgcgagccg ggctgcggcc accagacagg
     6541 acggacacca ggcggcagcc ggtggtggct gtcagccctt ggtgccagag ctgccaccgt
     6601 ccttgccctc ccccggcatc tggtcgcggt actcggccgc tgccttggtc cactcctcga
     6661 cgctcagccg ggcgggcacc accagcacgc ctccgacgtt gccgctcagg ttgacgtcgg
     6721 cgcggtcctt gtagtccggg ttgacccggc gcatctccat cgccaggatg ttggtggcgt
     6781 agaccttctt gtacgcgacg acctcgtcct tgtccttgcc tccgatgatc ggctcctcca
     6841 ctccctcgat ggagagcttg taggcgtggg ccatcaccct gtcccggtag gcgtccttgg
     6901 cgtcctggaa cgcggcggca aagtcggggt cgttctcaac gtggcttgtg acggtcggtg
     6961 gggtgacgcc ggccgcgcgc gccgagacat acatcaggcc gcactcggcg tacttctcaa
     7021 ggaagatgtt cttggcccgg tcgtcgaact tgagccggct ctcttgaagc cgcctgcgcc
     7081 agttgctcat gtcgacgacg cgaggaccgg ggccaccggt cgtcgaggca cgcggggtgc
     7141 cgtccttctt gttggccatc tggccatcct cctctgccct ggccccatgg cccaggcgat
     7201 cgccgcctcg cccatgcgcg cgtgatcctg gcgaaatatg tgagagacac ggcgcagagg
     7261 gcaagcccag agggagcggg gcgccactat cctgagaaac tggggtcgtc cgacatcgtc
     7321 cgacctttcc gacttaacga gttgtctttc tcgcaggtcg tgcggcctaa gcccttggcc
     7381 agccagggat agtctgacct tttagactta tagactaaga aagagagaaa gagggaatag
     7441 gcgccggccc agcgcccctg ctccctgggc ctagcgaaca gcaacccagt cgttccagtc
     7501 ccgaagtcgg acgatcgtca acttctccct gcgggatcag tgacttgcct cgcacgacct
     7561 atcgtccgac ctcggcgatc gtccgaccta gagccggctc gggtcgttcc agaagacatg
     7621 atacccgatg acgcacacgg gtgtcagctc ctccacccag cgtggtcgca cgtccactgt
     7681 gtggtaccac agcacccggc cgcagggccg ctcgcccgct ctcgcacgtc gcacggcacg
     7741 tctcgctcgc cggatcgccc tctccgtcca tgctctgtca gggagcgaga gaccctggaa
     7801 ctgggccggc tggtacacga cctcgcgcac cgtgtctggc cagcgccggt ccctggcccg
     7861 gtccagcgcc acaccggcga ccgcgatcat gccgtcgagt ggctcgccct cggcctcctg
     7921 cagcaccatc ctgacaaggt gctcgtctgc tctcagtggt ggctcctgag aaaggaccgc
     7981 cgaggagctg agcaggctca gcgccccggc ggccagtcgc accaccacag tgcatctcac
     8041 tacgcggcac tcccgaccag ggtcctggtc actggcatcg cgtcccagtg ctccaggtcc
     8101 cagccgtggt gccggcggat gtggtcgagg ccggccccgc tcagctcagg ccagggctct
     8161 cgctccgtcg cgttctctcg aaggtgcggc gccccgaggc ccaggtcggc gtagagcagc
     8221 cacatccagt ggaacgggta gcacaccgtg ccgaggtccg gccgcaggta gtgccactgg
     8281 ctcttgcggt tctcaggcag ggcgtgacgg atcgcgaact gagcccagct gcgctgcccg
     8341 tcctcgctat cgaacatgcc gccgaacacg gtctcgttgt gcagcagcca gtgctcgaag
     8401 ccgccctccg tggaccgccg catccgagcc gcgtactctg gatagccgcc gcccatgccg
     8461 tcgcggcaga acttgtacat cgagaagagg cggtcgatcg ggtggcgcac gacgccgacc
     8521 cggcgccagc ggtcgtaccc cacgggcacg ccgtcggcct ccatgtggcg gtagagctgg
     8581 taggcctgag ggtacttgcc cagcaccgcc cggcgcagcg agccgctgcc cgtcctgggc
     8641 accagcagca caaccgtctg tatctcaggt acgatgatca tcgccgctct cctcgaactt
     8701 gttctccctc agccactcac gcgcagcgtt gcaggggtcg ttgtgcccct tggcgatctc
     8761 tcggagcaga cctatggcgc tcttgccgta tatctccagc cgccgctcct gccatcgagc
     8821 ctcgctctga tcatggctcc tctgccgccg ctcgcgcacc gcttcgggat gatgctgctt
     8881 gcagtagccg tctcggaccg ccagtcgggt gcactggtgg aaggtcggcc accgccctcc
     8941 gtcgggcacg tgcctctcac ggcagcgtgg acgattgtca gtcatggtgg tgctcctctt
     9001 tcttctctgc taccagtacc gacggctgga ccgatggatc tcaagcccct ccgccttgac
     9061 gcgggtcagc tgcgtctcct tgacgccgtt gtactcgccg tggcgcttga ccgtgccgtc
     9121 gagagtgacc tcggcgcccg gctccacgtc cacatcgccc gaggcccacc acgtgagaac
     9181 gttgccgtcg gcggtcacca gcttgacaag ggtgctggcg ccgaagtcgc cacccttgct
     9241 cctcaccgac agcacgcggg cctgcacccc gcgcagccgc tccccctggg ctcccacgtg
     9301 gacggacttg agcagctccg cctccttggc cttgcgcagc tcccaggcgg acttgacccg
     9361 gagagcggtg gccagcgccg agcagacgat gcccaggtgg cgcgtcgagg cgaggtcgtg
     9421 cagcaccagc tggacgaggt tctcctcgta gtcgctgcgt ccctccttcg cggccaggtc
     9481 ctgctccacc cagcggatgg tctcaccggc ccggcgggtg tcatcgtccg tcaggccacc
     9541 cagcttgacg aggcgggtct tggcctcgga gcggacgacg gaggtccagg cgaagtggtc
     9601 ggccaccagg tcggcggtcg cccggtcgcc accgttgttg gccgccgagc ggctctgcca
     9661 gccgtaggcg cggatgtagg cggaggtcag ctccagggcc tccagcaccg gcacgtggtc
     9721 ggcgccgagg ccggagaacc agccgccgtc ctcgacgagg tcgctgacca tgcagagcca
     9781 ggtggcctgc gcggcggctc gctccggcga gttgatgccg gtgaagtcag ccaggcactg
     9841 gcggccgacc tgcagctcgc ggccgtcggc gtggcgcacg acaaacacgt cgcggcgacg
     9901 gcgggccttg cggcagtgct cgcacacatg ggcgcccagg gagcggaagc gcgggtcgac
     9961 ccggccctcc acacccggca ccgagtggat caggatgagg ccctcggcca gctcctcgac
    10021 gcgggcgacc agcgcccagt cggccagtcg cggggcagcc ccggccaccg tcacgaggcg
    10081 gacgggcacg tcgtgctccc gggagtgctc ctcgccctcg tacttgtaga cgacggtccg
    10141 cttgacgacc gcctcgccga ccaccgcgaa ggtgcatggc tcgcagccca gcttggcggc
    10201 ccggcggttc agcttggcga acttcgccac gaagtcgtcc cagcggacga cggggatctc
    10261 gaaggtgcgg atgtcctcgg tcatcgctca gccctccatc caggcgcgga gttcgccgtt
    10321 ctcaacgcgc tcgaccctca tcccatagcc acgggcgagg ttgcgcagct tgcgctcggc
    10381 accgcgaagg gtccggcagg gcagagccac ggcagatcgc gcgtcagtgg cggtcaccgc
    10441 gtagacgcca tagcgggcgt cctggcggcg gaggacgtac aggaggcgag gggtctctcg
    10501 ggtggtggtg gcgttcatgg tgttgctcct tttctcagac ttgcccctag tctgcctcgt
    10561 cttgcgccaa aaggcaagcc tcttgagaga gtatcgtcta aaatgccgtc tatccgtaga
    10621 tggtgtactc accgtgccgg aggtcggtga gcacggccac caccagccgg tggtcctcct
    10681 cggccagccg ctccagcccg aggacgttcc accagccgtc cagcgtcctg acaagggtgt
    10741 gggtgcctgg ccgggtggcg taggccggcc gccctcgggt gagccgggcg gtcagcccca
    10801 gctcggccca gagggcctcc aggaggtccc ggcgctcggc ctggggcagg gcgtagaggc
    10861 tgccaggacg ggagccgagc gggctccagc gcagtgcctc ctgggctcgc tcccggctca
    10921 tggctccctc ctcctgcgcc cgcctcgcgc cctcaggacc gcctcgtcga tcccagcctg
    10981 gcggcgccgc tcctcctcgg acagctccgg ccacttccgg ggctccctcg tggcggcctt
    11041 ggcggcgagc ctggctgccc gccgctgggc gcgcagccgg ccactgacac tcgcgatgct
    11101 caccgccgga tctcccggcc ggcccggcgg gtccgctcct cggcgtaggc caggaggcgg
    11161 ggcagctggc ccatcagctc ggcctcccgc tcggggttcc ggccgcccag ggcctgggcc
    11221 tcccggacgt agcgcagccc gagctgccgc tcggcgcagt cggtgcaggc tcggagggag
    11281 cggcggaggt aggcgaggga gagggtcatg agacaggctc cttctctcag gcgcgggcca
    11341 gctggtcgcg gtaggcccgc tggacccggc ggtaggtgac gccctcgggc tggcgcccga
    11401 tcgagcggag gaactggtca acctcctccg gggtccgccc gtcggcggcc attgcccggc
    11461 cgatctgacg gcaccagtcg agggaggcgg cgatggtctt ggtggcgctc acggcagtgc
    11521 tccttttctt ctcaactgag agaagtctgg cacatctctc tcaggaaggc aagcggcatg
    11581 tgcgactatc gcgcggcatt ttatcgatcc cgcgcccagg ccccaccgac cgccctggcg
    11641 gatcatggct cagtcctccc gctcggcgac ggggccggcc tgggtctccc ggaccttcag
    11701 gcccgcgcgg cgggcgcggc gctccagctc gatcggggcg gagaactggc ccaggctggg
    11761 ggtgaggacc cgaaccgtgc cgtcggccag ccgggcgcac acacgctcac cggtcgcggt
    11821 ggtctggagc cagaggcggg tgatgggggt cttggccacg gtggtgctcc ttttctctca
    11881 ggcagtgagg gaatcattcc acattccggc gagaaaggca agtgccttga gagactatcg
    11941 cgcggcattt tagacaatga gtgaggcccc agggcggggg acaccctggg gcctcggacg
    12001 gtgagctcag ccgggctagg gggaagctac gcctcggctg aggcctccct caccgccctc
    12061 cggccacgcc tccgcgtcct gccggaggcg gccgagcggg cgagatgaaa cctgccgctc
    12121 ccgcgctgaa cgttctcgat ccgcagtccc ttgccgcgca ggcggtcgat caggttccgg
    12181 atcgcacgct cggacatgga gcaggcctcg gcgaggtcgg acacggtggc gccccgctga
    12241 cgtgcaagga ggctggctac cttgcgttgg gtctcactga gaccatcgat cttcgacttg
    12301 gtcggcatcg tggctctcct tttctctgga ggaggccccg gatcggccca tccgtcagcg
    12361 gggacttctc caatggagat acatatacgc ctctatgcac caaaaggcaa gtccctcgag
    12421 atcagttact gagagtgagc cattcctccc cgtcccagta cggaacctga gacaggaccc
    12481 ggccgagagc ggcccagtcg cggacctcgt cgtagcggat ggcaatctca tcagcctcgc
    12541 ccctggcgag ccggccgagt gcctcgcggc gggccggccc caggagcggg gcgagggcgc
    12601 gcaccagctc ggtgcgtgtc acccgacgtt gccagcggca gggccgcccc agctgcagcg
    12661 gaccttgaca tactcctcgg agaggtccag ccggcggccg atgtgctgct cggccaggta
    12721 ggcctgaccg tacagaccgc actgcacaag gctgtcagcg gcggggccag ggtaggtctc
    12781 cacaccctcg gcgccggact ggcaggtgct gtgcgcgggg tgcagggcga gattgcagac
    12841 gagtatgaga accttgatca tgcgtctatg tccacgatgc tgcctcgttt gggggtgacg
    12901 ggctcgaccc gcccgtccgg gtgcaggatg acgtgcgacg gagctgggcc gggcggcagg
    12961 cactcgccaa gcacctcgcg cccgtcgacg aggcacggca ccatgagagg gcccaggccg
    13021 tcggtgtggc gccgggcact ctgcctgcgc cgcagcagca tctcctcgag cgggctcatc
    13081 ggtgctctcc tatgtggccc tgcagccagc gccacagagc cgggttgtct cggacgacct
    13141 gcatcagacc cttgcccatc gcgtcggcca ccatctcctc gacatcctgg cgcggcagcc
    13201 tgcgcttctc gaagaccgcc ttgtaggtct cgaccggcat gtagcagttg agaatggcat
    13261 gcgtcagctc gtgcagcagc acgcgggcga cctcctggcc gcgcagcctc cggtccagca
    13321 cgatgcacgg cgaccgctca gtgccgtcgt ggtacatgcc gacagcgttg tccgtctccg
    13381 cgatgtcctc gcggatgatg gggagggtct ggtggccgac gcgcaccttg ccgggcatcc
    13441 tcatgaggcg ggtctcctct tcctctcgcg ggcggctcgc ctgcgatccc tgtatccctc
    13501 cagcagagcc gaggcccgcc cacgcaccct gggccggtcg tcgatcagcg tgccgacctg
    13561 ccgcgcgtcg agcagtatgg cggtcgtggc gttgatgtgc ccgagatggt cgatcaggct
    13621 gtccgtcgcg gcgtcctcgc cgtccttgat gcaggcgatg tgccgctcga ttgcacccag
    13681 gtagctcatg agagagatcg gcttgtccct ccagttgtag ggtccgtact tctcggcccc
    13741 gtcgccgcag gccatggcgc cgtatatggc gccagcagcc ggcagcaggt gcatcgggac
    13801 gcggtccagc gccgcgatgt ccttcgggtt ggaggagctg gctctcttgc gctttgacat
    13861 tctcacccct gctgagctag agtagcaatg gggtgagtat gccggcccag ccgaggaggg
    13921 acaatccaat gatcgtccag gcgcagcggc tcctgctcat gcctcacact ccctgctgga
    13981 ccgcctccag gatcggctgc aggtcgggcg gggtgtagca gcgtcccttg accacccggc
    14041 cggcctcgtt cttgacaggt cggccgtgct ccagcttgct catgttggag cggtggacct
    14101 cccggaatgc gtccatgaag acggtgccaa ggcccaggga gagggtggtg ccgtcctgca
    14161 cgtagcgcag gtctgcgagg gcgtccaggc agccacgcag gtccccgctc gccatcccct
    14221 gggccagctc ggccagctcc tcctggagca gctggaggcg gagcaggagc agcccggccc
    14281 tcggcccgtg gatcctggcc tcggcgtgca ggtccttggc cagctcggcc atgctgtcgg
    14341 cgtatcgctg cagccggtcg gacatgtcct cgtcccaccc agggagggtg gggcggtcct
    14401 ctaccggtat ctcaaagaga cggtggaact cctccaccgc gtccagcgtc gcgcagccct
    14461 tgctcactcc tcgtcctcct ctgcctgcgg ccgcgccgcg attgccacca gagccatggc
    14521 cagcactgcc ggccagacaa gtgagagcca catgtaggcc ccggcgcctg gctctcgccc
    14581 gctcctacgc tcgttccagc tgcacagctc ggcaatcaga atgccgagag tgaggtagac
    14641 aatgaccagc tcgatgctca tgccatctcc gtatgcgctc agaccccgca gtccccgcag
    14701 atcgtcttga tctgcgctcg ctgctcctcc gtgaactgca gccagtccca ctgctcaccc
    14761 cgcagccgcg cgcacagcgc ctcgcgtgcc tggagccgct ctacctctgg cgtagccggg
    14821 tcgagtgtca ggtcgacgag gtcgccgagg tccgcctgca tgagatggtc gtatgtcatg
    14881 actgcatgtc ccctcagccc ggccatcatg gccaggacag gtttaatgaa ccgtaaacgg
    14941 cggctcgatg gtgccggtcc ggcgcccgct ctggtcgcgg actatgagac gcccagggtt
    15001 gactgggtcg ggctcgatgg tgccgacccg ccggccccgc tcgtcgcgga tgatgagatt
    15061 gtcaggattg atgggactgc tctcaatcgt cccaactctc cggccgctct cgtcgcggat
    15121 gatcaggtcc tccgccgtcg ccgtcgcgct cgcggcgagc gcgatcagca gcacgcccct
    15181 tccgaggatt gtccctgcgc tcatcgcgtg tcctcctctg cctgtgcagc gggtgcctca
    15241 gcgaggctgc tgctgggttg cgggttggtc tcctcatgcg atgggccgct cccactggtc
    15301 gtctcgcagc ggatgcacac cccattgcag gcgacggagc cggctgccat ccttgactag
    15361 cctgagcagg tcggctctga actcccggtc tctgtcggcc ctgcgcttca cgctccttgg
    15421 gatcctcgtc tcactcagca gctctgacag gtcgatcact ccgtgatcat cccggaacgc
    15481 tctcagccgc tcggccagtg taaccagcag ctgagcctcg tagttgtagc gcgggcagcc
    15541 tatcgtgagc accggccatg gccgctcgta gattggcggc aactcagcta tgaagccgcc
    15601 ccccaatccc aggtctacat ctatctcttg cgggccaaac cttcccagga aggctggctg
    15661 agtagcgtgg cgcacgcggc gcagtccatt gcggccatgc tcctggtgga ttacacaggc
    15721 atagagcatt ccgctcgggt cctcagagac catgaggttg cagccgtcga actccatgct
    15781 gtagggaccg tgctgctgga ggtagagaat gtcccaccgc tctgtgaact ccagacgaat
    15841 gccctcgctg tcgtgtgcaa tgtacgccag tcccatcaca caggctccat ctcgttgccc
    15901 cacgggtcca tagggaaccc atgctcgtcc agtgccttgg gagagatcac gaccgtgtac
    15961 ctctcgttct ccggcgactt gagccggatc cagcccgagc cacccttctg cttcctgggc
    16021 ttgcgcatcg gcgcgtgagg gtaggcgccg agccggccag ccatgacctc cagcgtcttg
    16081 tgcagctcac tgccggcctc ggcgtaggtg cttgtcatct tgccggcgag gccgaactcc
    16141 ttggccagct tccggaacag gcccttgtgc ccctccttga tgccgaccgc cgcgtggatc
    16201 agctcgtgga gcaggatgtc caggatgcgg atcggctcgg cctgcgtcgg gcagatgaag
    16261 atgtgcaccg ctccgtcggc cgcgacctcc gggtcgaagc actggccgat ggccgtcgca
    16321 tgacccttcg ggaagccaca ggagacctgg atcttctccg ggagggtgta gccacgctcc
    16381 cggaagaagg tcgcgtcgag ctggcccagg gcgtccctga gccagccctc gcgggtgtcg
    16441 tgtgacttgg tcatgcctct cgcccctgaa ggaatgcgac acccttctcg gacacggtca
    16501 ggctgtagcg accgccgtgc ctctgagcgt cgatcagacc cgcgttgatg gcgcggtgga
    16561 cagcattgta gccgtacatc agcgagccat gcggcccgat ggcgcgggcg accgggagga
    16621 tcgccgtgcc ggggttggcg cgcacgatca gaacggcctc gtgcatgcag ggaccaactc
    16681 tcatgctcat ggtggtgctc tccttttctc aggcctgctg cagcgcagcc aggctcgctc
    16741 cgttgacctc gccgtcccac cgggcctcgc cagaggcgag cacgtaggac agctccttga
    16801 ccacgcgacg gaggttccgc ccggtcgacc tcttggcacg gcggtcgccg cactgctgcc
    16861 agaggatcgt ctcgccggtg ttgctgttgg tgccgacgta gagcgtgttg cggatgccgc
    16921 tcttgaacac cagggtgccg acccggctgg ctgacttgtt gatcataccc gcctcccctt
    16981 accaggcccc ggtgagccgc tcgagaaggc gagccttggc cgacgccagc tcacgggcca
    17041 tctcgttgga ccaggggctg gtggaccgct ccgggtggcg agccgcgcgc aggcagtcct
    17101 cggtcagatg cttgacgacg tggtccagga acttgccgac gccctcctcg gtccgggcga
    17161 cgttcagctt gttgtccggc cccatcgcgt gcaggatctc ctgagccagc tgcagctcag
    17221 ctgcagcctg gtaggccgag ccggcccact ccagggcgaa gaacgggttc tggctcagct
    17281 tctcctggaa ctcggcgagc tgctcccagg cagtctcagc ctggtcctca aggtggcggc
    17341 ggagtagaga gagccgcccg agattggtct tgctggtgct catggtggtg ctccttttct
    17401 tggggcagcg ccccgtttca tgatccaatt ctgccccagg ccagcgagga agacaacacc
    17461 tttctgcgac gatcgcgcgg cattttagac atccctcgag caccccggcc ggcgccggcg
    17521 cgcggcccct agaagggcgt cccgcctcct ctgggctggg gcagctcccc ctgggcacca
    17581 ggcgcctcgg gccactcctg gcgcccgtac agctcctccc agcggcgcct gcagccctcg
    17641 agactgtcga tggtgtagaa ccgggtccgc tcccggactg tccgggtcca gccctccccg
    17701 gtctggatgt cccggtcggc gatccgctgg gtgtgcccga gcccagggac cacgcggtga
    17761 aggaagcggc cgagggcggt ctgcccagag cggcggttgg tgttgagctt gcgggtgtag
    17821 gcgatgtagt cgtcgaacag gtccttgatg acgacctcgc gctgccagcg gtcctccagc
    17881 agcaggcgcc cctccagcag cttgttgtac caccactcct cctcgaggct gagagacagc
    17941 agcttctgct ccagcagggc ctcggtgcgt ggcacgtcgc ggacctcgta cccgctcagg
    18001 tcgtaggtca gcagcatgtg aagcagcgcc tcgtacccgc cgctctccat ctgcctgatg
    18061 atctcgccga agtagcccgc gtcctgctga tgccccttgc caacgtccag caccaggaac
    18121 cgccgctcgt cgccgccggc cggcaccacg tgcatgtcgt tggacgccat gatgaggtgg
    18181 acgtagttgg gcgaggcctc ggcgtccacg cccttggcct cgatggtgat cgtctcctcg
    18241 gtgatgaggg tcttgaggat ggacgagtgc cgcttgtcgc ccgcgtagaa cgcctcgtcg
    18301 gcgaacagca gcaccacgtc gcgcaggtgc gcgttgaagt tgccgatgag gtgcgacggg
    18361 ttggatacct gcaggaagtg tcggcccagc aggtgtccga cgatcttggc gaagaacgac
    18421 ttgcccacgc cccgcccgcc acgcagcacg atggcgacct cgccgggtgt atcagggtgc
    18481 tgcaccagtc gcgccagcca gcccatcacg tagatgaaca gggtctcgtc gccctggcag
    18541 atgttctctc ggatgtgcgc gatgaacagc gagcagtcgc cgggccggct ctggcacgcg
    18601 aagcccttcc acatgttgta ggctcccttg acctcgtgcc caggggcgaa cacgatcgtc
    18661 tcgtactggc gacgatgctg gtgctgcagc caccacttgc cgacctgcat catgatgggc
    18721 aggccggtct tggggtcgtc gccgacctgt gtgcgctggt gcatgaagcg gtttcggaag
    18781 tcgtcgaagg actgccgcgt caggcgggtg cgcctcagcg ccgggtcgag gacctcctcg
    18841 atcacgcggc acttgccgcc gatgttgccg atgaccgcga actgctcgtt gagcttgcgc
    18901 agccagggat cctcgacctc ctccttggcc cgctcgatct gacggatggc atacttctcg
    18961 gcgttggctc ccttgtccag gacgctctcg ctgatggaga actctgggtc cgtgaccact
    19021 gagaagatga tgtcgtccgg cacgccagcg cggaccagct ggcagatggc gtcgaacagc
    19081 cacgcggagc gggagttgtc gccctccttg acctcgtccg ggtgcttgcc ctggacgatg
    19141 atgaccttga cccggtccgg cacgccccac tggtccagct cctcgatgcc agcgagccgc
    19201 tcgacgttgc cgctcacctt gacggtggcc ccgcctccga agccacgctc ctcgggcatc
    19261 tgcacgaccg gcgccggggt gaaccggctg agcggataga cgtggtcgtt gaagctcacc
    19321 agctcggcca gggccggctg ccgtcccttc ttgagcttgc gcgcgtccgg gatgttgacc
    19381 gtcccaggca gtcgcatgat gcggtcgatg ttgtggcagt tgtcggccgc gaacaggatc
    19441 tcgagctgct ggttgtaccg cttggcgtcc tcggccttgg ccaggtcgcc gtcgacctgg
    19501 atgggctgct ccaggcgcca gaagccctgg tagccgccgc cggagaacac gacgcaggtc
    19561 ggctccggca cgccctgggg cagcctggtc gtgagcagcg ccagcgcccg ctcgcgctcc
    19621 gcctgcaggt cctcgcccgc cctcgggtcg atgtcgacat ggagccacgc gaccgccctg
    19681 atgtcctcgc gctcggcctt cttggtgacg tcgcgcagga ccgggttgac gtggaagtag
    19741 atgttgcggt ggccgttgta ctgctccagc cactgctcca gctggtggag gctcgccggc
    19801 cggaaggtgg ccgttgcaat cgccttgcgg tccgcctgga tcgctgtgag gacccacggt
    19861 ccctcgggcg cccaccgctg gaggaatgcg agggccgcct tgttgtcagg cttcactgga
    19921 gccatggggt gacgatgatc cagaggagga agaggaagga gccgaccgca gctgagtaga
    19981 tcgccaccga cgtgaggcgg tcgaagacct cgacacaggg catgctactg ctcccagtac
    20041 cacagaaggg tgtcgcagcc gacgagaccc tgctccatct ggttcaccca gaaccggcag
    20101 acctcaagct cctcggccac gcgggcctgg gtgaagcctg cccgccgccg gtaaagaagg
    20161 caacgctcat gtgcggccag ctcgccgatc tcgacaggca tgccccagtc gacagcgtcg
    20221 cgctccatct tcccgtacat gctgtgcgtg aggccgagcc gacgcgccgc cgcgatctgc
    20281 ccctcgccgc gtctgcggcg atctatgagc aggcgctcgc ctcttgtcag ctcgccgagc
    20341 tctgtcttcc tgccagacat tccaccaact ccgagtgtcg cagtcctctt ggccagtagc
    20401 ggctggcacc ccgcagcagc gcacccctgg gcgcccggcc cacgatgatg gcggcggtct
    20461 caccgtcgaa gagcaggtac tcctgcccga cctgcagcag gagccaggcc agaccacccc
    20521 gacgacagcg acgagacagc cacactcgct gctgctgggt gaagtgcctg atgagaaccg
    20581 gatctgtgtc cgcgttctcc ggccaccgtc gcagccactt cagctccaac caaccaccta
    20641 cgtagttgac atcaggggtg ccagggtatg ctgggttctc aaccgctacc gcgtcgagtg
    20701 acctcagcgc gcggaccacc ctaccgcgca tcgtcttctc agacaagtgt gacctcgacg
    20761 ccgcactcct ggaagtagcg gagactgcgg atcagcaccc tctcccatcg ctccgccttg
    20821 tccgccggca gcgagggcga caccactctg gtgatcccgg cctgtatcat ctgcactgcg
    20881 cagcgatcgc agcagcagaa tggccaggtg tagagtgtgc agccggccac tggtccgtgc
    20941 gcgtgcagca cagcgttgat ctccgcgtgc acgacccggc tgtacttctc atctcgatcg
    21001 tggtacagcg cctcggcgtc gtacatcccc ctggggaagc cgttgaagcc cagggaggcg
    21061 acggtccggt cgggccggac aatcaccgcg ccacacttgg tcgacgggtc cctgctccac
    21121 tgtgccacga tctcggccag ctgcagaaac cgaatgtccc acttgtcctg actgctcact
    21181 ctgcctctcc ccagctcggg cctatctcca cgtccactct gaacgggacc tcggccggta
    21241 tgcaattgct cattacctct gccatccgca tcgcctcttc cctgtcccgg acggagctgt
    21301 ctagctcgtc gtggacctgc agctgcagga agtgccccat ccggtccagc tcgaccatgg
    21361 cggtctttgt ctggtcggcg ctggacccct ggatgacacg gttcagtgcc ttgtgcgtcc
    21421 actcatagct gccgtcacca cgggcaggga agtgcaggtg tcggccaccg atggtgacaa
    21481 catacccgcg cgccttggca acgtcctcgg ccttgcgtgc cagcttgcgg atgaacggcg
    21541 cccgcgcgtc gaagacatcg aggatgcgct ggccctcctc gcccgccgcg cgccacacga
    21601 agccctcccc tccctcctgg gcacgggcct ccagcgcctc ggcctgcgtg ctgaagtagg
    21661 caacgcgccg ctctctgccg cgccccatgg cgagagccca gcgggtgggc agccccaggt
    21721 cgtcgcacag cttcgcacca ccctcgccat agcacaggcc gaggtagatg ttcttggcgt
    21781 acttccggtc gaggccagtc agatccgcca tgaactggtg gttgtccagc ttggggttgt
    21841 cgtggtacgc ctgagccgcc tcgcgggcgc ccggcagatc catcattgcc gcgaagtgcg
    21901 tcgtccagcg aggctcctgc tgactgtagt cgcaggctgc ccagagagca ccctcctccg
    21961 gctcgtagat gctgcgccac atcttggcga actcatctcg gctcggctgc tgctggaggt
    22021 tgggatcgac gcagctcacg cgaccgtagc gggcaccctt ctgatcgccc ttgtcgtcct
    22081 cgcgtgccat ctggttgtag gtgcagtgga tgcggccgtt gaccatgtac gttcgcaccg
    22141 aggcggcgaa cgtcgtgcgc agcttgttga ccttgcgtgc ccacgcgagg gccttggcca
    22201 ccgggtggtc gatagaggac agcaggtcct tgtctatgct cggcttgccg gtgctggtct
    22261 ggtgcagctt gacgccgatg tagtgcagcg ccggggccag cgcatcggcc ttccacacgt
    22321 cgccgaccgc gatctgcaca ccggtctggt ctcgcaccag ctgcagcgcc tcagtctcct
    22381 tgtcgaggct ccacgtctcg atgccgcgca gccgctccat gttgacccgc acaccccggc
    22441 gccgcatgcg caccagcacg ggcagcactc ggctctccag gtcgtatatg cgccacaggt
    22501 cgcgctcgtc gatgatgcgc tcctggcggc ggagagttag cagcggctgc tccgtgtcga
    22561 cctctgcata ggggccgaca tagcgggccg gcagctgcca catgccgtgc ttggtggcga
    22621 caccgtatgc ccgagccgcc tgctccagca gctcctcgct cttgcccggg aggccgtggc
    22681 gcttggcgat cgcgtccaga ctgtagctca tctgcagctc gtcgatgagc ggctccgcga
    22741 tctgcacgtc gcggtacagc tcaacctgcg ggaagtagac accctcctcc cagaggtagt
    22801 ccaggtcgta cggcaggttc gccccgacca gctgtcctga gaacctggca gcgttctcgc
    22861 gcagataccc caagacctgc tcgaccggga ggttgtcacc gccctggtgc cgcattggca
    22921 ggtacgcccc tgggccgtcc tcgatcttga aggacacgcc gacgacgtac ccaccgcgcc
    22981 gcacaccgat gcccagctgg cgcaggtgcg ggtcgtaggt ctcgacgtcg acgcagaccc
    23041 tcttggcctc ggcccaggag ggcagcgacg agagatcggg aggggaccag ttgctctcag
    23101 gcgtgaacag gggcagctgg agcagccccg gccggttgct gttgcgcttt gccatcagtc
    23161 gatggagttc atgagccgga ggaaggcggc ctcgctgatg tagccgaccg tcgccagctc
    23221 gtctcggatc aggcgcataa gcgcggcacc accctctcct gcagcagggt ggcggttact
    23281 gaggtggtgc ctgccgtcgc cctcgccgta ataggagcac tgcacacggt tgtagagacc
    23341 atctctcccg agggtcttgc cattgcggcg tgccacgtgg ccggccagtg ggaagcgaaa
    23401 ctccaactct cgcaggaagc gagggaagtc ccacccgatg acggaccagt cgtttctgca
    23461 ggccacgaga tcgagatagt gtacctccat ctccaccacc acaactccga ggcagaggag
    23521 gaagtgcgtc tcgtggtgag acgcccactg gcggcggctc gccacgctct tgggcgactt
    23581 gcgagattgc ccactcctcc ggcgaactac cttgacgggc gacggtccct tgcgattgcc
    23641 aatcatgtcc cactcctgcg ctgctcatcg tcgtgcagca gcttggcgat cgctgctcgc
    23701 ttgatgttct ccgcgaggct ggtgccctgg cgctggacct cgctcggccg tccctcggcc
    23761 agggccgcca cgacctggcc gcagacgttt gtgaggacag tcagcgcgac ctgcaggtcg
    23821 tactcgctga gggcctcgac taccttgccg gccgcctcgt gcaccggatc gagcggctct
    23881 ggctgctccg ggcctcgcac tagcggatct cctcgtcggc gaagtagccg aacgggtgat
    23941 cctgcccgct ggtctttgtc tcgcccttct ctcgctccaa tcggatcatc tcggcgatct
    24001 cgtcgcgcgc ccgctccagg tcggatgggg agacccactg gcagagcagg cacatgatga
    24061 gcgtctcgcg ggccgtcagc tcgttggcca cggcgtagcg cagcaggtcc tcgaccgccg
    24121 cctggccacg gttcttgcgc ggcgccaccg gccggctgga cagcgccagc agcttgtcca
    24181 cgtagtgcat ggccttctcg agatcctgca ggccagcctt cttccgccac ctggtgacgt
    24241 acttggtggc gcagccctcc aggtagccca ggcccgcgtt ctcgatgagg tcccagtgct
    24301 ccatgtccgt cttgtagtgc ccgccgccga cctggcgctt gttcgggtcc tcgtggccgt
    24361 agcggtcctt ctctgccatg ctcactagct tctccagtgc tgttctgtcg ctgggcctca
    24421 ccctgtcctc gggctcgtca acccacttga tgcagtcccg gccgcgtggg cactcagggt
    24481 actcccaccg ctccaggccg cagccgcagc cacgatcgtg gctcacttgc aggcctcccg
    24541 catcacgcca gcctgcaccg gcagcagctc cgagccctgg atgtaggcca ggaactcgtc
    24601 ggccggcgcg ggcagctgga tgaggttggt gaagtagtac tccagctcct tgagcgtctc
    24661 ctcggcgaag cggttgccca tgagcatctg ctcgcggcac cagaggtaca gctccagccg
    24721 gtcgcaggtc ttgagcttct cgaggtcgtc cttgggcaga gcgtcgagcc gaggcagacc
    24781 catgcgctcg ctcaggttgt cctcgatccg cccgagctgc tggcgaagcc cagggacgaa
    24841 gttcatcgtc ggggctggca cgtctccgac ccaggcctcg ggcaggtcgt gcgacaggca
    24901 cacggcggct aggcgcggga agtcggctgg ccacaggtag tacatcagca tggccacgcc
    24961 ccagctgtgc gccgcgttgc tgtagctggt ggcgtgcggg atggcgtggc accgctccac
    25021 ccggccgccg cagcggctct ggacgaggtt gttgacgatc ccgtgcatct tactcggctg
    25081 catcgtggta ctccttggcg atggcctcgt ctcggcgccg gatgacccag ccgatggagg
    25141 ccgcctgcca gtctgtcgcc ctgcactgct gaagtatctc gagggccttg tcgaaccgat
    25201 cagggtctgc cttgtcctgc agggccttgt gcgcgcagta catcggcgtg accacgcggc
    25261 ggaagaatgg gtctcgcagg ccgacgatcg gaccgtggtc catgaacatc agcaggtcct
    25321 gctgccactc caggatcggc gtctggacca gcgggaacca ggagacctgc ccagaggcgt
    25381 aggggttgcg gccgctaagg cggaacgggt cagccgcctc gtccgccagg tgggccaccc
    25441 tcttgagctg atcctcatag gcgtgcaggt tgttggacat ctgccagtag gttcccactg
    25501 ggcagccgat ggcagcggcc atgaactcct gcagcacgga gaagtgcact gcgttcgcgc
    25561 cgtaggcgcc ccagatgagg tcgttggagc ggcagcagac cgtcatgtcc agcgcgctgg
    25621 aggagttgcg actgaagtag acctgggtgt tgcaggggaa gtcccgcccc tccttgccga
    25681 ggtcgacctc gcaatcccac atctgcagca cgcagcggcg gtcgtccggg ttgtcctgca
    25741 ggcgggcgac gatctcggcc agctggtcct ggccgaagtg ctgccgccag cggtggccgt
    25801 aggcagcgtg gaacgtcttc ccgttgtcgc tgaaggaccg catgcgcttg acgtactgcg
    25861 ccacgaaggc cacgtcgttg cggcccgcca gcatccacag gctctccatg aggtggaaga
    25921 acgggtttgc gtcccggtcc cgccagaaga tcacccgctc cctgggctcg tggtactcag
    25981 tcgtgaccgg gaaggggaag accctcactg ggccgttgcg ggagttgcgc ccctcgccca
    26041 ggcgagacag caggtccagg gcagtgggga gagcctcgtg gacgttgcgg accgttatga
    26101 cctgcatggc tcaggcggcc tcctgcaccg gcagaccgag catgcggttg agcacggtgg
    26161 tctggatggc gggaagctgc acggcgggct gtgtcagatc gccgcagaac ggggctgcct
    26221 ccggccagac ctccagcagc ttgccgacgg tggtcacggc attgagagcg gcgtcgatgc
    26281 cggccgtggc ctcgttgatg ccgcgcttca ggtccgtgag ccggttctcc agctcgtcgt
    26341 gccgctcggc gatggggtgg cgagcgtcgt acaccttcac caccctcttg tacttgtcgt
    26401 acggtaaggt gagctccgtc tgagggatgc tgtcccgcag gaaggcggtc acgttggagt
    26461 agagctggcc gtggaagggc agctcgtgca ccttgccagc gagctgcacc ttgatgacat
    26521 cgtccttcgg aagccagccg gcgggcaccg ccgccatctg cttcatgatg tccttgaagc
    26581 agtccgtgta gacctccttg gcgagagccg cgcgatcttg gatcagctgg tcgtaggtct
    26641 ccttgaagcg atgccgcagc atgcgcttcc gaatgatctc gcgcagtgca gtggtcagct
    26701 tgacgctcgc catctcaacc tccattgtat cgctgcttcg gggtgccctc gccgagacgg
    26761 gcacgctcgt acttgtcgaa ctcacatagc gtgtgctcga cctcgcgcat ctcccatttg
    26821 ggccagccag acggccagat gagcggattg cgagaacggg ccagcagcat ctgcatggcc
    26881 tcctgcatct cgtcgacctg gcacttgacg tggcggtcga accgctccgg ctcgccgtag
    26941 agcacccgcc cgaggccccg cgccgctcct gggccggggt tggcccagga gaggatgtct
    27001 gtggcctggt cgaggaggta ggtgtggcgc aggtcggtca cgacctcgta cgccatgaac
    27061 ggacccatgt aggggaactg ctggagcagc tcggttgcgt gctggagaga gcgggccgcc
    27121 agcatctcat gccccactcg ctcggcgtgt ggcacgaatg cctcgatgca ctccagcacg
    27181 ccctccagct tgttcttccc cgccggggtc ttgaccatgt aggcaccggt caccagcggc
    27241 ttgacatcgc gcagtctccg ctcagcgtag tcgcggttcc aggtcagcag caggttggtg
    27301 ttgctgaccg cccgcccctc cagccgctcc agcgtgggga tgcggttgaa ccaccgcgcg
    27361 atgtacatcg ccagcagcac ccgagggtcg cggcgcagcg gccggcggac gttctcggcg
    27421 atccaccgcg tgacgcggtc gtcctcgcgg aagacgttgc agaagcggta ctgccgcagg
    27481 atggggtcgt cggtccaggg agccggctgc ccggtctccc ggcgcagcat cacacggtac
    27541 cgctcgcggg cgtaggcgaa gaaccgctca ccgccgtgca tgctcacagc cccagctccg
    27601 ccctgatgcg cgccagcgcc gcgtcgcggt cgaggctgac agctggcacc ccggccgcct
    27661 ccagcctcct catgctctgc tgcacgccct tgtgcttgct ctcggtgttg cggggattga
    27721 ccggcggcag gtccgggttg cgctcacggc ggcgggcgtt gacgctgtcg aggcagacct
    27781 gcagcggcac atccagcgcg accaccagca tcgggcagcc ctgctcctgg gcgaacgccc
    27841 agagggcggc ggtcctgttg acgtccgcgg agatcagaag gccctcgaag accacgtcgc
    27901 agccgttgtc gtgggccttg cgcgccagct cgaagatgtg ctccatcttg gagatggtgt
    27961 cgcagccacc gcaggtggtc tcatagtggc cgatcac
//
LOCUS       DAOQYE010000002        11315 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_6190_length_11315_cov_3.346536, whole genome shotgun sequence.
ACCESSION   DAOQYE010000002 DAOQYE010000000
VERSION     DAOQYE010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 11315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11315
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6190_length_11315_cov_3.346536"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="PKN52_00235"
     CDS             <1..391
                     /locus_tag="PKN52_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98389.1"
                     /translation="HPGADRKPLPQGAGGHVHAGGAVQLHERGPAGGARVGVGGEHGD
                     GLLEREDVAKLGIVPQGVEEALEAFVKTAGGFKRFELALEQLREPGALDLASGSVLVR
                     ILEQARTAANAGVAKRAGAVDPRPYGP"
     gene            complement(379..1515)
                     /gene="hemW"
                     /locus_tag="PKN52_00240"
     CDS             complement(379..1515)
                     /gene="hemW"
                     /locus_tag="PKN52_00240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HNQ98390.1"
                     /translation="MVTPALYVHVPFCPSICPYCDFHKMLRNEGLVARYVARLEAEAA
                     AVSAAFPGSPATVYLGGGTPSHLTDAELTRVFAALRDGWGEATGRAETTLEADPLTFD
                     EGRLTTFNALGVTRLSIGLQSADDATLRFLGRVHDAAAGLAAARLALAEGFRVNVDVI
                     MAVPGQDVESDLRRVADLGARHVSVYSLTVEPGTPFARRGVRVDPEADADAFELAGQV
                     LAEYGLRRYEVSNFAAPGEESLHNLTYWRGEPYLGIGPSASAYLPVGGPFGVRYKNPT
                     IKAWLAGEPPEVETLSADDYLLERLMTGLRTREGVDLEWLERRVGTTLATVAPTWLHD
                     VTRHALLERVEGRLRATPTGLERLDGVLRAFVGSRTPIAIDQGP"
     gene            complement(1646..2995)
                     /locus_tag="PKN52_00245"
     CDS             complement(1646..2995)
                     /locus_tag="PKN52_00245"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNQ98391.1"
                     /translation="MKRITPLLGAVLALALAVPAFAQDWDNVDPSGQTVTFWHQHTRE
                     RETALQEIVAQFNATNPYGITVVAEYQGGYNDIYQKMVALLGTSDTPNIVVAYQNQAA
                     TYQLVDGLVDMNPLVDSAKWGISAEDQADFFPGFWNSDIFPSFGGQRLGIAPNRSMEV
                     LYYNADWLKELKSAGLIDFDGPPTTPEQFEAAACAASQTAFSGATASGTPFGFELSID
                     ASRFASFTFAFGGNVYDAAENRYTLDSDAAVQAMTWIQGLFAKGCATMVSEAYGDQTD
                     FGNGRLLFAVSSSSGLPFYQTAIDSGADFDWSVAAIPHTTAEPVMNVYGASVSLPSGH
                     SVESTLASWLFLKYYTDTEAQTEWAIASQYFPVRQSVAAGLGEFFDSLPPFKTAFELL
                     PYGIAEPNVPGYDPVRERINSAMADITDGDDVASTLQLLNGEANLILDDQLSQLPTN"
     gene            complement(3101..4051)
                     /locus_tag="PKN52_00250"
     CDS             complement(3101..4051)
                     /locus_tag="PKN52_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNQ98392.1"
                     /translation="MAEPRARGKGEAAPTAQVVATTQVIGGVERVVRPSNLRQPYHFE
                     LAHIPIYFLLIVGAVISVTPFFYMVSTSLMTLGETINRVLLPASPQWANYEVAWSEAN
                     FALYFRNSVIITALVIVGVLVTCTLAAYAFARIRFPGRELIFTVLLATLMIPGTVTFI
                     PNLLLVRGQILPWGSWLNTLPALTVPFMATAFTIFLLRQFFVGIPGELYDAARIDGAG
                     HLRFLTSVVIPMSKPVMMTATLLTFVSSWNEFLWPLLVTTTPKWRPLGVGLYTFISEA
                     GPETQLLMAGTVITVIPVLIVYFFTQRQFTEGIATSGLKG"
     gene            complement(4044..5231)
                     /locus_tag="PKN52_00255"
     CDS             complement(4044..5231)
                     /locus_tag="PKN52_00255"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNQ98393.1"
                     /translation="MKLVAAGPAQRPVRRRSSRQDRLDWLTAYLFILPATLLIAVFGL
                     FPIGYSVYMSLYNWRIRQGDFVGLRNYEATLGDWWGALAFFGGIVLIIVAHWLWTDAF
                     KGDRVRAPQAARIVGALVLLGAGVFMALGWRLMMDAGSKVYLKGLQRTVYYGFGSVPI
                     QIALALVLATLLFQRIRGQEFFRMLYFLPYVTPAVAGAAIFRSLFSPREERLANQILD
                     WLGIAPQRWLFETKPVTQLIFGNLFPNADWSGFWAGPSLALITIIIFGIWTFVGYNAV
                     IFLAGLGGISKELYDAAAIDGANRAQQFRFITVPLLSPVTFYLTVLGFIGTFQAFTHI
                     YVMRERATRDAVDTASIVIFDTFYKNNNFSLAAAQSVILFVIILLLTLLQNRMFGRRA
                     LGG"
     gene            5293..6327
                     /locus_tag="PKN52_00260"
     CDS             5293..6327
                     /locus_tag="PKN52_00260"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HNQ98394.1"
                     /translation="MPVSRYFVEVPAGSEVVARAELQRLGARRLAEFPGGFRLVQDAD
                     LPKALRARTVSAAYLSITFPVPRPKALLGDANFRALVAAVKGVAARGGYDGLRLSAAG
                     ADSPTLTRLAKDLAAAAGLPWLQEDGELLVRLRPDPSGNGWEALVRLTPRPSSTRAWR
                     VCNRPGGLNAAVAAVMNELTGTQAQDRYLNMMCGSGTLLIERALRAPARVLVGFDIDR
                     EAVACSEANAAAARVAKRCELLAGDLTDQRFLEELGARGRFDVFTADVPWGDAVGAHA
                     ANRALYPKVFAAAAALAEPGAALCILSHELKLLRGVVRDQPWWRVERELQVAHGGHHP
                     LVLLLSRVPE"
     gene            6369..7154
                     /locus_tag="PKN52_00265"
     CDS             6369..7154
                     /locus_tag="PKN52_00265"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNQ98395.1"
                     /translation="MSVKRLVPSSLRGVRPGWYVLFAIAVYALVVTVLLVARRPPAAG
                     VAGHAGATVTAATAVAGSASGATATAGLASGLWFPIPGARLPAQDDHLPGAARAYRHG
                     VSQGFDFFDQDAGVPVVAGTPVVAAQAGQVVRADVAYTEIDPRAWEVLMADVAERGAD
                     EGQLDQLRGRQVWLRLSDGTVLRYGHLQAVRDGIRTGVNVYRGQVIGFVGNSGTDVAV
                     AGRTDQARLRFEVWPGAGTDEFFGAGLDAEAVRIAAASLFVGP"
     gene            7178..8434
                     /locus_tag="PKN52_00270"
     CDS             7178..8434
                     /locus_tag="PKN52_00270"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNQ98396.1"
                     /translation="MQPEQRAKAKHERTALETVWQNVWVRAVSYIVLTVVLVLVLWRL
                     RHGYAFALQVGLIGFMIAYILNPVVDWLTRLRLRRNLAVIVTYALLLLVMAFGSVIIS
                     QVVMELGRFVQLVPTAIERLTQWTGQFQEWLVSISDRLPSFLSDRLGVSDDSGAISIQ
                     VREQVASFFQQLVDGLRSLLESLVTGGPGFLLTGATAVISTTFQVFLVFLAGAYFLYD
                     FPRFIANFRRFVPVRYRELASDIGAKADLAVGGYLRGQLLITMLLGVMIWAGLSLIGI
                     PLATAIGFLAAVFNLVPYLGPILGVTPAILLGFTVSPLAAVLAAVVFLVANQLEAHVL
                     SPLILSRNTNLHPVTVMLAIMAGIGLMGLLGALLAVPTVALIKVLADDYLLTRPAFAS
                     GPPVGDAPEPKATKGRKAPPNRRRKR"
     gene            complement(8458..10839)
                     /locus_tag="PKN52_00275"
     CDS             complement(8458..10839)
                     /locus_tag="PKN52_00275"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNQ98397.1"
                     /translation="MTQGKPGTARSALKLGPSAMALVWASVPVGVFLATYPLGGVGLW
                     HGFLLAIPFMVATSGFGWRVGLALAPVGLGLLWLRTSLDGTGATPSAYALLLAVMALA
                     AIAGDRFYQAWRGAQRQAGQAGNRAKLLQEAAVDLNHAADVDSLFASAPRLLSDILSF
                     AHASVFVPHGSALRVHTTLRWGAEPHFEVPLESVMGRAYATGRPQYVVDTTSDPDYIP
                     APGAELTRSEFALPVVVDGKVRAVLNLEHRTKDAFSPADHATLTAFVRMMEEVIARLD
                     ATSALNETTREQQFMARLNERLLLAEGLMEAADVALDEVLSWFGLDLGAVLEMHRARL
                     RPVAARGEPPPVLAQRLRDGLEFVGVLRHAWETREIVLVDDLAQEGVWTTTTEARALA
                     AVPIVNPSGQVQALLVVARYREPLPTWSDRDRALLSSISAPLGAAFSRMTLNRQLLAT
                     LDAIRQLRSAAGPDALYHHAAQAAVDLVPNAEAASILVRHGDHYYYEAAVGYDLETLQ
                     AHAGPFTYAEQLTWYQGSDEDFDAGRARVLRGPSIAAASLTSRRTPVGVVPARMSEMR
                     CQLAVPIVDMGRVVALLNLDNFSTEDAFSGAALRVAEAFAQHITVVVRQAEELVLLER
                     SAVTDALTGLGNREGFERTFRQELARARRLERHLCLMLVDLDDFKQINDRFGHAEGDK
                     ALVSVAKALAGAARAGDHVFRWGGDEFALLLPESHPDEARAAAGRFRDVLAGLEVNGV
                     RLMASFGLADYPIDGIDCDALMSIADHRMYADKRKKTGAGKPRKTGAPANLPG"
     gene            10959..>11315
                     /locus_tag="PKN52_00280"
     CDS             10959..>11315
                     /locus_tag="PKN52_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98398.1"
                     /translation="MKKNTRRERKRVEASRLDLEAVGLIMLAAGAFLAGVLLPLLPSG
                     ELGRTVRGALTGRLGVGAYALPVPLVILGALFLVRRNPRWWPRATIGYLLAAAGVWGV
                     LAAAAPETAGAWGAALR"
BASE COUNT         1730 a         3762 c         4071 g         1752 t
ORIGIN      
        1 ccatcccggc gccgaccgaa agcccctgcc ccaaggagcc ggtggacatg tccacgccgg
       61 gggtgctgtg cagttgcacg agcgtgggcc ggcgggaggc gcgcgcgtag gcgtcggcgg
      121 cgagcacggc gacggactcc tggagcgtga ggatgtagcg aaactcggga tagtcccgca
      181 gggcgtcgag gaagccctcg aggcgttcgt caagaccgcg ggcggcttca agcggttcga
      241 gctggcgctc gagcagctca gggagcccgg ggcgctcgat ctcgcctcgg ggtccgttct
      301 cgtcaggatc ctggagcagg cgaggacggc cgcgaacgcg ggggtggcca agcgggcggg
      361 ggcggtcgat cctaggccct acgggccctg atcgatggcg atcggagtcc tgctgcccac
      421 gaaggcccgc agcactccgt ccaaccgctc gagccccgtc ggggtggccc gcaagcgccc
      481 ttcgacccgc tcgagcaagg cgtgccgtgt cacgtcgtgc aaccacgtcg gcgcgaccgt
      541 ggcgagcgtc gtgccaaccc gacgctccag ccactccaag tcgacgccct cgcgcgtgcg
      601 taggcccgtc atgaggcgct ccaggaggta gtcgtccgcg ctcagcgtct cgacttcggg
      661 cggttctccg gccagccacg ccttgatcgt cgggttcttg tagcggacgc cgaacgggcc
      721 gcctaccggc aggtacgcgc tggcggacgg cccgatgccc aggtacggct cgccgcgcca
      781 gtacgtgagg ttgtgtagcg actcctcgcc cggagcggcg aagttgctca cctcgtagcg
      841 ccgcaggccg tactcggcca ggacctggcc ggccagctcg aacgcgtccg cgtccgcctc
      901 cgggtcgacc ctcacgccgc ggcgcgcgaa cggcgtgccc ggctccacgg tcaggctgta
      961 gacgctgacg tggcgcgctc ccaggtcggc cacgcgccgc aggtcgctct cgacgtcctg
     1021 accaggcacc gccatgatga cgtcgacgtt cacgcggaaa ccctcggcga gggcgagccg
     1081 cgcggcggca agcccggcgg cggcgtcgtg gacgcggccg aggaagcgca gcgtggcgtc
     1141 gtcagcggac tgcaggccga tggagaggcg tgtgacgccg agcgcgttga acgtcgtcag
     1201 gcgcccctcg tcgaacgtga gggggtcggc ctcgagggtc gtctcggccc ggccggtcgc
     1261 ctcgccccag ccgtcgcgca aggccgcgaa gacacgcgtc agctcggcgt ccgtcaggtg
     1321 cgacggcgtg ccgccaccca ggtagacggt ggcggggctc cccgggaagg cggccgagac
     1381 cgccgcggct tcggcctcga ggcgtgcgac gtaacgcgcc accagcccct cgttgcgcag
     1441 catcttgtgg aagtcgcagt acgggcagat gctcggacag aacgggacgt gcacgtagag
     1501 ggccggcgta accacgtgcc aaggctacgc cacgcggcgc gcgccgggcc gcatgcgctc
     1561 caacgcgtgc gctccaaaga gtgtgggcgg cgcgatcgtc gcgccgccca cactgatgcc
     1621 gagccgccga agggcgggac ccgactcagt tcgtcggaag ctgggagagt tggtcgtcga
     1681 ggatcaggtt cgcctcgccg ttgaggagct ggagggtgct ggcgacgtcg tcaccgtcgg
     1741 tgatatccgc catcgcggag ttgatgcgct cacgcaccgg gtcgtagccg ggcacgttgg
     1801 gctcggcgat gccgtacggc aggagttcga acgccgtctt gaagggcggc agcgagtcga
     1861 agaactcgcc gaggccggcg gccacgctct ggcggacggg gaagtactgg ctggcgatgg
     1921 cccactcggt ctgggcttcc gtgtccgtgt agtacttgag gaacagccac gacgcgaggg
     1981 tggactcgac gctgtgcccg gagggcaggc tcacgctggc gccgtagacg ttcatgacgg
     2041 gctcggccgt ggtgtgcggg atggcggcga cgctccagtc gaagtcggcg ccggaatcga
     2101 tggccgtctg gtagaacggc aggcccgagc tggaggagac ggcgaagagc aggcggccgt
     2161 tgccgaagtc ggtctggtcg ccgtacgcct cgctcaccat cgtggcgcac cccttggcga
     2221 acaggccctg gatccaggtc atggcctgga cggcggcgtc ggagtcgagc gtgtagcggt
     2281 tctcggccgc gtcgtacacg ttgccaccga aagcgaacgt gaaggaagcg aagcggctgg
     2341 cgtcgatgga gagctcgaag ccgaacggcg tgccggaggc ggtggcgccg gagaacgcgg
     2401 tctgggaggc ggcgcacgcg gcagcctcga actgctctgg agtggtgggc gggccgtcga
     2461 agtcgatcag gccggcgctc ttgagttcct tgagccagtc ggcgttgtag tacagcactt
     2521 ccatggaacg gttcggggcg atgccgaggc gttggccgcc gaagctcggg aagatgtcgg
     2581 agttccagaa gccggggaag aagtcggcct ggtcctcggc cgagatgccc cacttggcgg
     2641 agtcgacgag cgggttcatg tcgacgaggc cgtcgacgag ctggtacgtg gcggcctggt
     2701 tctgataggc gacgacgatg ttgggcgtgt ccgacgtgcc gaggagcgcc accatcttct
     2761 gatagatgtc gttgtagccg ccctggtact cggcgacgac ggtgatgccg tacgggttcg
     2821 tggcgttgaa ctgcgcgacg atctcctgga gggctgtctc gcgctcccgg gtgtgctggt
     2881 gccagaacgt tacggtctgg ccgctggggt cgacgttgtc ccaatcctgc gcgaacgcgg
     2941 ggaccgccaa cgccagggcc aacacggcac cgagcaacgg tgtgatcctc ttcatgaccg
     3001 actcctttcg agtagggcgt ctcaccctag ttacgcctaa gcgtagcgcg ctgacagcgc
     3061 cgagtcaccc tataggggca acgtcatggt cacgtaagct tcaccctttc aggcccgagg
     3121 tcgctatgcc ctcggtgaac tgcctctggg tgaagaagta gacgatgagg accgggatga
     3181 cggtgatgac ggtccctgcc atgaggagct gcgtctccgg cccggcctcg gagatgaagg
     3241 tgtagaggcc gaccccgagc gggcgccact tcggcgtggt ggtgacgagc agcggccaca
     3301 ggaactcgtt ccagctcgag acgaacgtga ggagcgtcgc cgtcatcatg acgggcttgc
     3361 tcatggggat gacgacggac gtcaggaagc gcagatgacc agccccgtcg atccgggcgg
     3421 cgtcgtagag ctcgccgggg atgccgacga agaactgcct gagcaggaag atggtgaagg
     3481 cggtcgccat gaacggcacg gtcagcgctg gcagggtgtt caaccacgac ccccacggga
     3541 ggatctggcc cctcacgagg agcaggttgg ggatgaacgt gacggtgccc gggatcatca
     3601 gcgtggccag caacaccgtg aagatgagct cgcgccccgg gaagcggatg cgcgcgaacg
     3661 cgtaggcggc cagcgtgcac gtgaccagca cgcctactat caccagggcc gtgatgatga
     3721 cgctgttgcg gaagtagagg gcgaagttcg cctcgctcca agcgacctcg tagttggccc
     3781 actggggcga ggcggggagg agcacgcggt tgatggtctc gccgagcgtc atcagcgacg
     3841 tggagaccat gtagaagaac ggggtcacgc tgatgacggc cccgacgatg agcaggaagt
     3901 agatgggtat gtgagccagc tcgaaatggt agggctgcct caggttgctc gggcgcacca
     3961 cgcgctccac gccaccgatc acctgggtgg tcgcgaccac ctgggcggtc ggcgccgcct
     4021 cgcccttgcc acgtgccctc ggctcagcca cccagggccc tcctgccgaa catgcggttc
     4081 tgcagcagcg ttagcagcag gatgatcacg aacaggatca ccgactgggc cgccgccagg
     4141 ctgaagttgt tgttcttgta gaacgtgtcg aagatgacga tgctggccgt gtcgacggcg
     4201 tcccgggttg cgcgctcccg catgacgtag atgtgcgtga aggcttggaa cgtgccgatg
     4261 aagccgagca ccgtcaggta gaaggtgacg ggggagagga gcggcacggt gatgaaccgg
     4321 aactgctgcg cgcggttcgc gccgtcgatg gccgcggcgt cgtagagctc cttcgagatg
     4381 ccgccgaggc cggcgaggaa gatgacggcg ttgtacccga cgaaggtcca gatgccgaag
     4441 atgatgatcg tgatgagcgc cagcgaggga cccgcccaga agccgctcca atcggcgttg
     4501 gggaagaggt tgccgaagat gagctgggtg acgggcttcg tctcgaacag ccagcgctgc
     4561 ggcgcgatgc cgagccagtc gaggatctga ttggccagcc gctcctcgcg cgggctgaac
     4621 aggctgcgga agatcgccgc gcccgccacc gctggcgtga cgtacggtag gaagtagagc
     4681 atgcggaaga actcctggcc gcgtatgcgc tggaacagga gggtggcgag cacgagggcg
     4741 agggctatct ggatgggcac ggagccgaag ccgtagtaca ccgtgcgctg gagacccttc
     4801 aggtacacct tgctgcccgc gtccatcatg aggcgccagc cgagggccat gaacacgccg
     4861 gccccgagca gcacgagagc ccccacgatc cttgccgcct gcggcgccct gaccctgtcg
     4921 cccttgaacg cgtccgtcca gagccagtgg gccacgatga tgagcacgat cccgccgaag
     4981 aacgcgagcg cgccccacca gtcgccaagc gtggcctcgt agttgcgcag cccgacgaag
     5041 tcgccctggc ggatgcgcca gttgtacagg ctcatgtaga cggagtagcc gatcgggaag
     5101 agcccgaaga cggcgatgag caaggtggcg gggaggatga agagataggc cgtcagccaa
     5161 tcgagccgat cctggcgtga cgaacgccgt cggacggggc gctgggctgg gccggccgcc
     5221 acgagcttca cgctgtaact atagccgaga ctccacgctc gggtcgcccg gcggccctgc
     5281 tagcatgtgt gggtgccggt atctcgctac ttcgtcgaag tgccggctgg cagcgaggtc
     5341 gtcgcgcggg ccgagctcca acgcctcggc gccagacgtc tggccgagtt cccaggaggg
     5401 ttccgcctgg tgcaggacgc ggacctgccc aaggcgttgc gcgccaggac cgtcagcgcc
     5461 gcgtacctct ccatcacctt ccccgtgccg cgccccaagg ctctcctcgg cgacgcgaac
     5521 ttccgggcgt tggtcgccgc cgtcaagggc gtcgcggcgc gcggcgggta cgacggactg
     5581 cggctttccg ccgccggggc cgactcgccg accctgacgc gcctcgcgaa ggacttggcc
     5641 gctgccgccg gcctaccgtg gctgcaggaa gacggcgagc tgctcgtgcg cctgcgaccc
     5701 gacccgtccg gcaacggctg ggaggccctc gtcaggttga cgccgcggcc gagctcgaca
     5761 cgggcctggc gcgtctgcaa caggccaggc gggctcaacg ccgccgtggc cgccgtcatg
     5821 aacgaactga ctggcacgca ggcgcaggac cgttacctca acatgatgtg cggctcgggc
     5881 accctgctca tagaaagggc gctgcgagcc ccggcacgcg ttctggtcgg cttcgacatc
     5941 gaccgcgagg ccgtggcctg ttccgaagcg aacgccgcgg ccgcgcgcgt cgccaagcgg
     6001 tgcgagctcc tcgccggcga ccttacggac cagcggttcc tcgaggagct cggggcgagg
     6061 ggccggttcg acgtcttcac cgccgacgtg ccttggggcg acgccgtcgg cgcgcatgcc
     6121 gccaaccggg ccctctaccc gaaggtgttc gcagccgcgg cggcgctggc cgaaccaggc
     6181 gccgcgctct gcatcctgag ccatgagctg aaactcctgc gcggggtggt ccgagaccag
     6241 ccgtggtggc gcgtcgagcg cgagttgcag gtggctcacg gcggtcacca cccgctggtc
     6301 ctgctcctct cgcgcgtgcc ggagtaacgt cctgggcggt aagaagagcc gtgctaaact
     6361 tcgcctccat gagcgtgaag cgcttggtcc catcgtcgct gcgcggcgtc aggccgggct
     6421 ggtacgttct gttcgccatc gccgtctacg cgctagtggt caccgtactg ctcgttgcca
     6481 ggcgcccgcc tgccgccggg gtagccggcc atgccggcgc caccgttacc gctgccacgg
     6541 ctgtcgccgg cagcgcctcc ggcgctactg cgacagccgg cctcgcttcc gggctctggt
     6601 tccccatccc cggagcgcgg ctgcccgcgc aggacgacca ccttcccggg gcggcgcgcg
     6661 cgtaccgtca cggcgtgagc cagggcttcg atttcttcga tcaggacgcc ggggtccccg
     6721 tcgtcgccgg cacgccggtc gtcgccgccc aggccggcca ggtcgtgcgg gccgacgtcg
     6781 cctacacgga gatagacccg cgcgcttggg aggtcctgat ggccgacgtc gcggagcgcg
     6841 gcgccgacga gggccagctc gaccagttgc ggggcaggca ggtctggctg cgcctgtccg
     6901 acggtacggt gctccgctac ggtcacctcc aggcggtgcg cgacggtatc cggacgggcg
     6961 tgaacgtcta ccgcggccag gtcatcggtt tcgtcggcaa ctccggcacg gacgtcgcgg
     7021 tggccggccg gacggatcag gcgcgcctgc gcttcgaggt gtggccgggg gcggggaccg
     7081 acgagttctt cggcgccggc ctggacgctg aggctgttcg catagcggcg gcctcgttgt
     7141 tcgtcggacc atgatgggcc aggaggtcga gcgagcggtg cagcccgaac agcgagccaa
     7201 agccaagcac gagcggacgg cgctcgagac cgtatggcag aacgtctggg tgcgggcggt
     7261 gagctacatc gtgctcaccg tcgtcctcgt cctcgtgctg tggcgcctca gacacggtta
     7321 cgccttcgcg ttgcaggtcg gcctcatcgg cttcatgatc gcctacatcc tcaaccctgt
     7381 cgtggactgg ttgacgcgac tgcggttgag gaggaacctc gcggtcatcg tgacctacgc
     7441 gctgctcctg ctcgtgatgg ccttcgggtc cgtcatcatc agccaggtcg tcatggaact
     7501 gggccggttc gtccagctgg tgcccacggc tatcgagagg ctcacgcagt ggacggggca
     7561 gttccaggag tggctggtga gcatctccga tcgcctgcca tccttcctga gcgaccgtct
     7621 gggcgtgtcg gacgacagcg gggcgatcag catccaggtg agggagcagg tggcctcgtt
     7681 cttccagcag ctcgtcgacg gtctgcgcag cctcctcgag tccctcgtca ccggcggccc
     7741 cggcttcctc ctcaccggcg cgacggccgt catctcgacg accttccagg tcttcctcgt
     7801 cttcctcgcc ggcgcctact tcctctacga cttcccgcgc ttcatcgcga acttccggcg
     7861 cttcgtgccc gtgcgctacc gtgagctggc gagcgacatc ggcgccaagg ccgacctggc
     7921 cgtcggaggc taccttcgcg gtcagttgct catcacgatg ctgctcggcg tgatgatctg
     7981 ggcgggcctg agcctgatcg gcatcccgct ggccaccgct atcggcttcc tggccgccgt
     8041 cttcaacctc gtgccgtacc tcggcccgat cctcggcgtg acgccggcca tcctactcgg
     8101 cttcaccgtc agtccactgg ccgcggtgct ggcggcggtc gtcttcttgg tggccaacca
     8161 gctcgaggcg cacgtgctct ctcccctcat cctctcgagg aacacgaacc tgcaccccgt
     8221 cacggtgatg ctggccatca tggccggcat cgggctcatg ggtctcctcg gggcgctgct
     8281 cgccgtgcct acggtcgccc tcatcaaggt cctcgccgac gactacctgc taacgcgccc
     8341 ggcgttcgcg agcgggccac cggtcggcga cgcacccgag ccgaaggcga cgaaggggcg
     8401 caaggccccg ccgaatcgcc ggcggaagcg ctgagagcgc caggagctcg gcggcgctca
     8461 gccggggagg ttggcgggcg cgccggtctt acgcggcttg cctgccccgg tcttcttgcg
     8521 tttgtcggcg tacatgcggt ggtcggcgat gctcatcagg gcgtcgcagt cgatcccgtc
     8581 gatcgggtag tcggccaggc cgaagctcgc catcagcctg acgccgttca cctccagtcc
     8641 agccagaacg tctctgaagc gtccggccgc cgcccgcgcc tcgtctgggt ggctctcagg
     8701 gagcaggagg gcgaactcgt cccctcccca acggaacacg tgatcgccgg cgcgggcggc
     8761 gcccgcgagc gccttggcga cgctcaccag cgccttgtcg ccctcggcat gcccgaaccg
     8821 gtcgttgatc tgtttgaagt cgtccaggtc gacgagcatg aggcagaggt ggcgctccag
     8881 acggcgcgcc ctggcgagtt cttgcctgaa cgtgcgctcg aacccctcgc ggttgccgag
     8941 gccggtcagc gcgtccgtga cggcggagcg ctcgagcaag acgagctcct ccgcctggcg
     9001 tacgacgacc gttatgtgtt gggcgaacgc ctcggccacg cgcagcgcgg cgccgctgaa
     9061 ggcgtcctct gtcgagaagt tgtcgaggtt gaggagcgcg accacgcgac ccatgtcgac
     9121 gatgggcacg gcgagttggc agcgcatctc ggacatgcgc gccggcacca cgccaacggg
     9181 cgtgcggcgc gaggtcaggc tggcggcggc gatgctcgga ccacgcagga cccgggcgcg
     9241 accggcatcg aagtcctcgt ccgaaccctg gtaccaggtg agttgctcgg cgtaggtgaa
     9301 cggcccggcg tgcgcctgga gcgtctcgag gtcgtaaccg acggcggcct cgtagtagta
     9361 atgatcgccg tggcgcacca ggatggaggc agcctccgcg ttcggcacca agtcgaccgc
     9421 cgcctgcgcg gcatggtggt agagcgcgtc ggggccggct gccgaccgga gctgcctgat
     9481 ggcgtccagg gtggcaagca gttggcggtt gagcgtcatg cggctgaacg ccgcgcccaa
     9541 cggggcggag atggacgaga gcagggcgcg gtcgcggtcg ctccacgtcg gcaacggctc
     9601 ccggtaccgt gccacgacga ggagggcctg aacctgaccg ctcgggttga cgatgggtac
     9661 ggcggccagg gcccgcgctt ccgtggtggt cgtccagacg ccctcctgcg ccaggtcgtc
     9721 cacgaggacg atctcccgcg tctcccaggc gtgccgcagc acgccaacga actcgagtcc
     9781 gtcgcgcaaa cgttgcgcca gcacgggtgg gggctcgccc cgcgcggcta ccggccgtag
     9841 ccgcgcgcgg tgcatctcca ggaccgcgcc gaggtcgaga ccgaaccagc tcagcacttc
     9901 gtcgagcgcg acgtcggccg cctccatgag cccttcggcg aggaggaggc gctcgttgag
     9961 gcgcgccatg aactgctgct cgcgcgtcgt ctcgttgagg gcgctggtgg cgtcgaggcg
    10021 cgcgatgacc tcctccatca tccgtacgaa cgccgtgagc gtcgcatggt cggctggcga
    10081 gaaggcgtcc ttggtgcggt gctccaggtt gagcacggcg cgcaccttcc cgtcgaccac
    10141 cacaggcaag gcgaactcgc tccgcgtcag ttccgcgccg ggcgccggga tgtaatccgg
    10201 gtcgctcgtg gtgtcgacga cgtactgggg ccgcccggtc gcgtacgccc tgcccatgac
    10261 gctttcgagc gggacctcga agtgcggctc ggctccccat cgcagggtcg tatggaccct
    10321 gagcgcgctg ccgtgcggca cgaacacgga ggcgtgcgcg aacgacaaga tgtccgacag
    10381 tagccgcggt gccgatgcga acagggagtc gacgtcggcc gcgtggttca ggtcgacggc
    10441 cgcctcttgg aggagcttgg cgcggttgcc ggcctgcccc gcctgacgct gcgccccgcg
    10501 ccacgcctgg tagaagcggt cgccggcgat cgccgccaac gccatgacgg cgaggagcag
    10561 cgcgtaagcg gaaggcgtgg cccccgtgcc gtcgaggctc gtacgtagcc agagcagccc
    10621 gagcccgaca ggcgcgagcg cgagaccgac gcgccaaccg aaccccgagg tggccaccat
    10681 gaaggggatg gcgagtagga agccgtgcca gagtccaacg ccccccagcg ggtaggtggc
    10741 gaggaagaca ccgacaggga ccgaggccca caccagcgcc atggcgctgg gacccaactt
    10801 gagcgcggac ctagcggttc caggcttgcc ttgtgtcatc ggatggatca tctcaccccg
    10861 gctcttacgg ccgcattacc tggcgaaaca ggcgggctcg cacgtaagcg tgggaaagct
    10921 ctgtctgggc ccggcggcaa gggcggtaca ctggcggcgt gaagaagaac acccgacggg
    10981 aacgcaagcg cgtcgaggcg agccggctcg atctcgaggc ggtcgggctg atcatgctgg
    11041 ctgccggcgc cttcctcgcg ggggtgctcc tcccgctcct gccgtcaggc gagctggggc
    11101 gcaccgtgcg cggcgcgctc accggtcgcc ttggggtcgg ggcctacgcg ctgcccgtcc
    11161 cgttggtcat cctcggcgcc ctcttcctcg tgcgtcgcaa cccgcggtgg tggccgcgcg
    11221 ccaccatcgg ctacctcctc gcggcggcgg gcgtgtgggg cgtcctcgcg gccgcggcgc
    11281 ccgagaccgc cggcgcgtgg ggtgccgcgc tgcgt
//
LOCUS       DAOQYE010000003        10120 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_7110_length_10120_cov_5.224441, whole genome shotgun sequence.
ACCESSION   DAOQYE010000003 DAOQYE010000000
VERSION     DAOQYE010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 10120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10120
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7110_length_10120_cov_5.224441"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3191)
                     /locus_tag="PKN52_00285"
     CDS             complement(<1..3191)
                     /locus_tag="PKN52_00285"
                     /inference="COORDINATES: protein motif:HMM:NF024939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HNQ98399.1"
                     /translation="MAQLVVSAAVNIGVGLLLNFLFPPKGQDVEGPRLNDLSVTSSAY
                     GQTIPLHYGTVRTGGNVIWGPPIEEVTNTSRQKAGGKGGGGKVTTTTYTYFASFAIAF
                     GEGVADTILRMWADGKLVYDKTGGSPEVGGLKFRFYRGTETQLPDPLIEASEGEGNVP
                     AHRGLCYMVAERWPLADFGNRIPNVTVELTMAAEQLAPYTPMTLLPGANIPGSANGAD
                     SNSHMYLDPFTDDLYMFKTGASRSSVAAMSMQVYSGASGLNDYGARFCMNNKIYIQTG
                     SSNFEPFVEIDPVSMEPTGFSIGVDSSIGSWGGYLQFPNGCWQGVLAVGAPEIGIDLV
                     PVWVVSGNSVLGDGGFVVIYRDVATGQLVQDNYISGYDIGRGGVVIQDHFNRRLFVCQ
                     ESNTQLDLYEVVSTIVVGQFGPAVTPPVVRLVASYTKGGVDLAGTSDIRGWVVLPDEQ
                     ALILSNDSSMIKVDMETGVVLAQNLTVGHQYEDGWTTTGIFAFNRGSSVSTDIYTIDT
                     ETLQVIETQSLALCNFPDGKLNNYNSGAYDPRNHSIIISRVHPDSTPSPSSERVVRVL
                     LGRANGQGADLADIVADLSRRAGLADSEFDVTDLIGHEVLGYSVTRQAPLRSAIEPLM
                     RGYLFEGAESDWQVKFVMRGHAPVMTIEEEYVGRLQTDAEPIKEVRTQEIELPERFYV
                     RYSDRDQDYQQGTASDRRVSLPVPSQHSRNEVTLDLPIVGQATPMRQLAQRWLYLSWA
                     ERASIEAVVPWRYIRLDPTDVLEMEHRGDLRRLRAASVEVGADLALSLRTTQEDARAN
                     DSDLQGAGSLGYIPQVIPSSLPTRLLALDLPTLTAQEASIGSFSRAYWAAAGYDSTWP
                     GCVLYRSRDLGGSFEEISATSEEACWGVVVGTIPDPGTVYTWDDSSTITLRVARHIER
                     FTSATEAEVLNGANAIAVIRSDGLPEIIQFVTATQIDAATVQLSRLLRGRRGTEPCSR
                     GHGLAEQFVLLEEGSIVAFQLPLELVGVATQLRAATLGTPLEDATPIFKTYTGQDLLP
                     YSVAQVESERDAVTGDLTISWLRRTRFGGGLRDG"
     gene            complement(3193..3618)
                     /locus_tag="PKN52_00290"
     CDS             complement(3193..3618)
                     /locus_tag="PKN52_00290"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HNQ98400.1"
                     /translation="MSTLEDLAVKEAKTWIGTPYKHLGRDRFGLDCAGLIIKVAHSLG
                     ISDYDTANYSRRPNPQEFLRELRGKMDRVPKGELGHGMVGVFREPSHPCHVGILERDE
                     EGRLFVIHSYAPARKVVREPLTEERMSRLLMVFRYRETA"
     gene            complement(3615..4724)
                     /locus_tag="PKN52_00295"
     CDS             complement(3615..4724)
                     /locus_tag="PKN52_00295"
                     /inference="COORDINATES: protein motif:HMM:TIGR02218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2163 domain-containing protein"
                     /protein_id="HNQ98401.1"
                     /translation="MKDASAGLNAHLQQEVTTLATCWRVTRTDGVSFYFTDHDRDLVV
                     DLADGDGPQTYEASSSYNRSAIKNDDTLAVDNLDLTGVLSSEKIDELALRRGLFDYAR
                     IEVFIVNWSDLTQGVLKMRRGWLGEVTITPNGWFQAELRGLTQAYSRRSGELYTTECR
                     ADLGDHRCKIPIWPNLVARSTAYAVGDYVRLAGVGRVFRCTTAGTTAVAEPAYNTAVG
                     ATTTDDTAVFKAELAWSHTFTVVAVGADARKMFTVTELTPSSGGTIPGRDHFPEDSLN
                     GGVVVWETGANAGRAMEVRDFVADNGVVIEQSIELFLDMPLDIQVGDTGRVYRGCSKR
                     AIEDCKTIFNNIVNFRGEPYIPGQDRLFSYPDAKA"
     gene            complement(4721..5386)
                     /locus_tag="PKN52_00300"
     CDS             complement(4721..5386)
                     /locus_tag="PKN52_00300"
                     /inference="COORDINATES: protein motif:HMM:TIGR02217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2460 domain-containing protein"
                     /protein_id="HNQ98402.1"
                     /translation="MAFHEVRLPSDVERGAVGGPRFKTTVLQLASGFEQRNIDWEQVK
                     GEWDISYGLMSLRDNELDTYIHAVRDFFYARSGRAHGFRFKDWSDFEIGEPSDPLGGH
                     QLIGLGDGATTQFQVFKRYSSGGVSYDRVIKKLVSGTVSVLLDSVVQLAGYSVDVNTG
                     LVTFDVAPAATGGSGPGGEVVVQVACEFDVPVRFDDDHLKITVQTAQAGQIPAIPLVE
                     IRV"
     gene            complement(5386..7950)
                     /locus_tag="PKN52_00305"
     CDS             complement(5386..7950)
                     /locus_tag="PKN52_00305"
                     /inference="COORDINATES: protein motif:HMM:NF040129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HNQ98403.1"
                     /translation="MATERIDIVVTERGARTVRRNIEDIGSGAQRAQGAVSLLHRALL
                     ALGGAAAVQQLVSLLDTFTNLQNRMRLVTNGTAQLNVVTAELFDIANRTRVSFEGTAE
                     IFNRVALAAKDLGVSQQEVLNFTESLNQAIILSGASAAEAQAGMIQLAQGMASGTLRG
                     DELRSVLEQLPAVADVIAKSLGVTRGELRQMGADGLITADTILTAFREARGELGDQFA
                     KTVPTIGQAFTVLRNNVIELLGSFDRATGIAGVLARAILFIADNVDTLARAFAAAAIV
                     VGTVFAARAVGAAITAVQALTAAMVANPIGAIAVALTTVASLLITFSDRIMLSTDSLA
                     NLQDLGAAVFEELSAAVSSLVSFFSEGFALISSYAGDFFGDMDLSVAGLLRGAAWVID
                     RYIGLYVGAFRAIVAVWDNLPDAFRDIFSRALNGAISLVETGINKIISAINAVTEFTG
                     LGSIGAVTLGRVESSAAGGAARLGEAVKDGFLEGFNQTAVTDTVNRVISRAEQLAQER
                     IAQEQLTAAEEALARQQLGQAGERNVIATMDEDAIKKSEKQRDILRELIADLELEHQL
                     LQLTSREREVRGVILQTEAQLGRELTAVEEANLEALLQRNQLLREEEQLLQDINGPAE
                     DYANNIATLERLLARGAISQEQFNRAMRDARIAMLDQNTDVASGLERGFLRAQSQMED
                     FASSSERLITDAFANAQDAVVNFFETGKFQADDFFRSLANNFLRLGTQQLFSAAFGSG
                     GPLGGLGSILGGGGGGGGIGGLVTSLLGFKDGGSFTVGPDTSVGNVPGIDNRLIAFRA
                     RDGENVTINRPGESGARPVQVNFNITTPDAESFRRSQGQILARTSAALQRANSRNN"
     gene            complement(7951..8307)
                     /locus_tag="PKN52_00310"
     CDS             complement(7951..8307)
                     /locus_tag="PKN52_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98404.1"
                     /translation="MEEGASRTVRQVALAVSQAVIVSTPVDTGRARANWVASLGAPFT
                     TTTEDTDPSGSRQVAINNTVISGRQTGQEVYISNNLPYIGRLNEGHSAQAPAGFVEKA
                     VQRGTVAASKARVLER"
     gene            complement(8342..8632)
                     /locus_tag="PKN52_00315"
     CDS             complement(8342..8632)
                     /locus_tag="PKN52_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98405.1"
                     /translation="MRERMPLPERIRNAPQLEMGLELYYGAFFDLHTCRGGWGDGYIP
                     WTAIDEYCDRLGVSDDQREDMHYYIREMDEQYHQHREAEAESRRGGKGAKPS"
     gene            complement(8695..9117)
                     /locus_tag="PKN52_00320"
     CDS             complement(8695..9117)
                     /locus_tag="PKN52_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98406.1"
                     /translation="MYKLFKTDPNLEKAGIVVDYGDFRVTLARAGGGNRRFAQVLEAK
                     TKPFKRAIQTEMMDNERGLQLLRETYAEAVILNWEVRQGEDWVQGIEAPDGSVMEFSV
                     ANVLETFKNLPDLFTDLQEQASKAALFRQTVRENDAGN"
     gene            complement(9187..>10120)
                     /locus_tag="PKN52_00325"
     CDS             complement(9187..>10120)
                     /locus_tag="PKN52_00325"
                     /inference="COORDINATES: protein motif:HMM:NF039819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HNQ98407.1"
                     /translation="LDFTTLGLIPGEWVFVGGDLAAEAFDETENNGLKRIRSIAANRI
                     EFDKSALAMTTDAGAGKTIRLYFGRVLKNELGSDVIRRSYNIERTLGAPETSLPNQIQ
                     AEYLVGAVPSEISINVPQANKLTLDVSFMATDNEQREAAEGLKAGSRPAIVEADAFNT
                     SSDFVRLKMSKVEADEAPVALFAFLTELTITINNNLTPNKAVAVLGAFDVTAGTFQVG
                     GNVTAYFSDIAAVKAVRENADVTIDMALVKANSGMVIDIPLVALGDGRLNVQQDQAIQ
                     LPLSMDAATAAKIDPDLDYTAMWVFFDYLPDLAG"
BASE COUNT         1670 a         3317 c         3313 g         1820 t
ORIGIN      
        1 ccgtcgcgca gcccgccgcc gaagcgggtg cggcgcagcc aggagatggt caggtcgccc
       61 gtcacagcgt cccgctcgct ctccacctgg gcgaccgagt acggcaggag gtcttgacct
      121 gtgtaggtct tgaagatcgg ggtagcgtcc tctagaggag tgcccagagt agcggcgcgc
      181 agctgcgtgg ccacgccgac cagctccagc ggcagctgga aggccacaat gctgccctcc
      241 tccagcagga cgaactgctc tgccagcccg tggccacgag agcacggctc ggtgcccctt
      301 cgaccacgca ggagccgaga cagctgtacc gtcgcggcgt ctatctgcgt agcagtgacg
      361 aactggatta tctctgggag accgtcagag cgtatcactg cgatcgcgtt ggcaccattg
      421 aggacctcgg cctcagtggc agaggtaaac cgctcaatgt gccttgcaac gcgcagagtg
      481 atggtgctgc tatcgtccca ggtgtagacc gtgccaggat ccggtatggt gccgaccacc
      541 acgccccagc aggcctcctc tgaggtcgca ctgatctcct cgaacgatcc tccgagatcg
      601 cgagagcggt agaggacgca gccgggccac gtgctgtcgt accctgcagc ggcccagtag
      661 gcgcgactga aggaccctat gctcgcctct tgggccgtga gtgttggcag gtccagtgcc
      721 aacagccgcg tgggcagcga ggacgggatt acctgcggga tgtagccaag ggagccggcg
      781 ccctggaggt cgctgtcgtt ggcgcgggcg tcctcctgcg tggttcggag cgagagtgcg
      841 aggtccgccc ctacctcgac gctggctgcc cggagccggc ggaggtcacc ccggtgctcc
      901 atctccagca cgtcggtcgg gtccaggcgg atgtagcgcc atggaaccac tgcctctatg
      961 ctggcccgct cggcccagga gaggtacagc caccgctgcg cgagctggcg catcggcgtg
     1021 gcctgcccaa cgatcggcag gtccagcgtg acctcgttgc gggagtgctg cgagggcacg
     1081 ggcaggctca cgcgtcggtc ggacgcggtg ccctgctggt agtcctggtc cctgtcagag
     1141 tagcgcacgt agaaccgctc gggcagctcg atctcctggg tgcggacctc cttgataggc
     1201 tccgcgtccg tctgcagccg gccgacgtac tcttcctcga tcgtcatgac cggggcgtgg
     1261 ccgcgcatga cgaacttcac ctgccagtcg ctctctgcac cctcgaacag atagccacgc
     1321 ataagcggct caatggctga gcggagcggc gcctgccggg tcacgctgta gccgaggacc
     1381 tcatggccga tcagatcggt gacatcgaac tcgctgtccg cgaggccggc gcgacgtgac
     1441 aggtcggcca cgatgtcggc gaggtccgcc ccctgcccgt tagcccgtcc cagcaggacg
     1501 cggacaactc gctcggagct gggggatggc gtgctgtccg ggtgcactct ggagatgatg
     1561 atggagtgat tgcgcggatc gtaggcgccg ctgttgtagt tgttcagctt gccgtccggg
     1621 aagttgcaca gggcgagcga ctgcgtctcg atgacctgga gcgtctcggt gtcgatggtg
     1681 tagatgtcgg tgctgacgct gctgccacgg ttgaaggcga agatgccagt agttgtccag
     1741 ccgtcctcgt actggtgccc gacggtgagg ttctgcgcca gcaccacacc ggtctccatg
     1801 tcgaccttga tcatggagct gtcgttggag aggatgaggg cctgctcgtc gggcagcacc
     1861 acccagccac gaatgtcact ggtgccagcg aggtccaccc cgcccttggt gtagctcgcc
     1921 accagccgca ccacgggcgg agtgaccgcc ggtccgaact gcccaacgac gatcgtcgac
     1981 acgacctcgt acaggtcaag ctgtgtgttg ctctcctggc agacgaatag acgtcgattg
     2041 aagtgatcct gaattaccac cccgccgcgc cctatgtcgt atcctgagat ataattgtcc
     2101 tggacgagct gacctgttgc gacatccctg tagattacga caaagccacc gtcaccgagt
     2161 acagagtttc ctgaaaccac ccagactggc accaagtcga taccgatctc tggtgctcca
     2221 accgctagaa caccctgcca gcagccgttg ggaaactgca gatagccacc ccaacttccg
     2281 atggagctat caacaccaat ggagaaacca gttggctcca ttgagacagg gtctatctcg
     2341 acgaacggtt cgaagttgga ggagcctgtc tgaatgtaga tcttgttgtt catgcagaac
     2401 cgggcgccgt agtcgttgag cccgcttgcg ccgctgtaga cctgcatgct catggcggcg
     2461 accgaggagc gagaggcacc ggtcttgaac atatacaggt catctgtgaa cgggtccagg
     2521 tacatgtggc tattgctatc tgcgccattt gcagagccgg gaatattcgc gccagggagc
     2581 agcgtcatgg gagtatacgg agcgagctgc tccgccgcca tggtcaactc tactgtaacg
     2641 ttcgggatgc ggttgccgaa gtcagccagc ggccaccgct ccgccaccat gtagcagaga
     2701 ccacggtgtg ccggcacgtt gccctcaccc tcgctcgcct cgatgagtgg gtctggcagc
     2761 tgcgtctccg ttcccctata gaacctgaac ttcaatcccc cgacctctgg acttccaccg
     2821 gtcttgtcat ataccagctt gccatcagcc cacatgcgca ggatggtgtc cgccacgccc
     2881 tctccgaagg cgatcgcgaa ggacgcgaag taggtatagg tggttgtcgt caccttgcca
     2941 ccaccaccct tcccgccagc cttctgcctg gaggtattgg tgacctcctc gatgggtggc
     3001 ccccagatca cattgccacc cgttcgcacg gtgccatagt gcagtgggat ggtctggcca
     3061 taggcagagg atgtaacact gagatcgttg agacgcggcc cctcgacatc ctgccccttc
     3121 ggggggaaca ggaagttgag cagcagcccg acgccaatgt tgacggcggc agagacgacc
     3181 agctgggcca tgctacgcgg tctcccggta gcggaacacc atgagcaggc ggctcatgcg
     3241 ctcctcggtg agcggctcac ggacaacctt gcgcgccggg gcgtacgagt ggatgacgaa
     3301 gagccgaccc tcctcgtctc gctcgaggat gccgacgtgg cacggatgtg acggctcccg
     3361 gaacaccccc accatgccat gacccagctc ccccttgggc acccggtcca tcttccccct
     3421 cagctcccgc aggaactcct gcgggttggg ccggcgagag tagttggccg tgtcatagtc
     3481 gctgatgccc agggagtgtg caaccttgat gatcagccca gcgcagtcga ggccgaagcg
     3541 gtcgcgccca aggtgcttgt agggcgtccc gatccaggtc ttcgcctcct tgacagcaag
     3601 gtcctcgagc gtgctcatgc cttggcatcc gggtagctga agagacggtc ctgcccaggg
     3661 atgtagggct cgcctcggaa gtttacaata ttgttgaata tcgtcttgca gtcctcgatg
     3721 gcgcgcttgg agcatcctcg atagacacga ccggtgtcgc cgacctggat gtcgagcggc
     3781 atgtccagga acagctcgat gctctgctct atcaccacgc cattgtcggc gacgaagtcg
     3841 cgaacctcca tggcccggcc tgcgttggca ccggtctccc agacgaccac gccaccgttc
     3901 aggctgtcct ccgggaagtg atcacggcca gggatggtgc caccggagct gggggttagc
     3961 tcggtgacag tgaacatctt gcgggcgtcc gcgccgacgg ccaccacggt gaaggtgtgg
     4021 ctccaggcca gctcagcctt gaacactgca gtgtcgtcgg tggtcgtcgc cccgacggca
     4081 gtgttgtatg ctggctccgc gaccgcagtg gtgccggccg tggtgcagcg gaacacccgg
     4141 cccaccccgg ccagacgcac gtagtcgccc acagcatagg ccgtgctgcg cgccaccagg
     4201 ttcggccaga tggggatctt gcagcggtgg tcgccgaggt cggcgcggca ctccgtcgtg
     4261 tacagctccc cgctccgccg gctgtaggcc tgcgtgaggc ctcgcagctc agcctggaac
     4321 cagccgttgg gggtgatggt tacctcgccc agccagcctc ggcgcatctt gagcacgccc
     4381 tgcgtgaggt cggaccagtt gacaatgaag acctcgatgc gcgcgtagtc gaacaggccg
     4441 cgccgcagcg ccagctcgtc gatcttctcg ctcgacagca ccccggtcag gtccaggttg
     4501 tctacggcga gcgtgtcgtc gttcttgatg gccgagcggt tgtaggagga cgaggcctcg
     4561 taggtctgag gcccgtcccc atccgcgagg tccaccacaa ggtccctgtc gtggtcggtg
     4621 aagtagaagc tcacaccgtc cgtgcgcgtg acgcgccagc aggtggccag cgtcgtgacc
     4681 tcctgctgca ggtgggcgtt caggccggcg ctggcgtcct tcatacacgg atctccacga
     4741 gagggatggc cgggatctga ccagcctgcg ccgtctgcac ggtgatcttc aggtggtcgt
     4801 cgtcgaagcg caccggcacg tcgaactcgc acgcgacctg caccacgacc tcccctccgg
     4861 ggccggagcc gcccgtcgcg gccggcgcca cgtcgaaggt gacgaggccg gtgttcacgt
     4921 cgacgctgta gccggcgagc tgcaccacgc tgtccagcaa cactgagacg gtgccactca
     4981 ccagcttctt gatgaccctg tcgtagctca ctccgcccga ggagtatcgc ttgaagacct
     5041 ggaactgcgt agtcgcgccg tcgcccaggc cgatgagctg gtggccgccc agcgggtcgg
     5101 agggttcgcc gatctcgaag tcggaccagt ccttgaaacg gaagccgtga gctcggccag
     5161 agcgagcgta gaagaagtcg cgcaccgcgt ggatgtaggt gtccagctca ttgtcgcgca
     5221 gcgacatcag gccgtaggag atgtcccact cgcccttcac ctgctcccag tcgatgttgc
     5281 gctgctcgaa gccagaggcc agctggagga cggtggtctt gaagcgaggg ccgccgacgg
     5341 cgccacgctc cacgtcggag ggaagcctta cctcgtggaa cgccatcagt tgttcctcga
     5401 gttggcccgc tgcagcgccg cgctggtgcg ggcaagtatc tggccctgag accgacggaa
     5461 gctctcggca tccggggtcg tgatattgaa gttcacctgc accggtcgcg ctccactctc
     5521 tccggggcga ttgatggtca cattctcgcc atcgcgcgca cggaaggcaa tcaaacggtt
     5581 gtcgatgcca gggacgttgc cgacggaagt gtcaggcccc acagtgaagc tgccgccatc
     5641 cttgaagcct aggaggctgg tcacgagacc gccgatgcct ccgccacccc cacccccgcc
     5701 gaggatgctg ccaaggccgc cgagtgggcc gccggagccg aaggcagccg agaacagctg
     5761 ctgggtgccc aggcggagga agttgttggc cagcgagcgg aagaagtcgt cggcctggaa
     5821 cttgccggtc tcaaagaagt tcaccaccgc gtcctgggca ttggcaaagg cgtcggtgat
     5881 gagacgctcc gaggagctgg caaagtcctc catctgcgac tgcgcgcgga ggaagccacg
     5941 ctccaggccg gaggcgacgt cggtgttctg gtccagcatg gcgatgcggg cgtcgcgcat
     6001 cgcacggttg aactgctcct gcgagatcgc gccgcgcgcc agcagccgct ccagcgtggc
     6061 gatgttgttg gcgtagtcct ccgccgggcc gttgatgtcc tggagcagct gctcctcctc
     6121 gcgcaggagc tggttgcgct gcagcagcgc ctccaggttg gcctcctcca cggcggtcag
     6181 ctcccggccg agctgcgcct cggtctgcag gatgacgccc cggacctccc gctcacggct
     6241 ggtcagctgc agcagctggt gctccagctc caggtcggcg atgagctccc tcagtatgtc
     6301 gcgctgcttc tccgacttct tgatcgcgtc ctcgtccatg gtcgcgatga cgttgcgctc
     6361 acccgcctgg cccagctgct ggcgagccag cgcctcctcg gcggcggtca gctgctcctg
     6421 ggcaatccgc tcctgggcca gctgctcggc tcggctgatc acccggttga cggtgtcagt
     6481 gacggcggtc tggttgaagc cctccaggaa gccgtccttc acggcctcgc caagtcgggc
     6541 cgccccgccg gccgcgctgc tctcgacccg gcccagggtg accgcgccga tgctgcccag
     6601 gccggtgaac tctgtcaccg cgttgatggc gctgatgatc ttgttgatgc cggtctcaac
     6661 caggctgatg gcgccgttca gcgcgcggct gaagatgtcg cggaaggcgt ccggcaggtt
     6721 gtcccagacc gccacgatgg cgcggaaggc gccgacgtag aggccgatgt agcggtcgat
     6781 gacccacgcg gccccgcgca gcaggccggc gaccgacagg tccatgtcgc cgaagaagtc
     6841 acctgcgtag gagctgatga gcgcgaagcc ctcgctgaag aacgacacga ggctgctgac
     6901 cgccgcgctg agctcctcga ataccgctgc gccgaggtcc tgcaggttgg cgaggctgtc
     6961 cgtggacagc atgatgcgat cggagaaggt gatgagcagc gaggcgaccg tggtcagcgc
     7021 caccgcgatc gcgccgatcg ggttggccac catggccgcc gtcagcgcct gcaccgcagt
     7081 gatggccgcc cccaccgcac gcgcggcgaa caccgtgccg accacgatgg cggccgcagc
     7141 gaaggcccgc gccagcgtgt caacgttgtc ggcgatgaac aggatcgccc gagcgagcac
     7201 accggcgatg ccagtggcac ggtcgaagga gccgagcagc tcgatgacgt tgttgcgcag
     7261 gacggtgaac gcctgcccga tcgtcggcac ggtcttggcg aactggtcgc ccagctcgcc
     7321 cctcgcctcc cggaacgccg tgaggatggt gtcggccgtg atgaggccgt cggcgcccat
     7381 ctggcgcagc tcaccacggg tgacgcccag ggacttggcg atgacgtcgg cgaccgccgg
     7441 cagctgctcc aggaccgacc gcagctcgtc gccgcgcagc gtgcccgagg ccatgccctg
     7501 ggccagctgg atcatgcccg cctgggcctc cgccgccgag gcaccggaca ggatgatggc
     7561 ctggttgagg ctctcagtga agttgagaac ctcctgctgg ctgacaccaa ggtccttggc
     7621 ggccagtgcg acgcggttga agatctccgc cgtgccctca aatgagacgc gcgttcggtt
     7681 cgcgatgtcg aacagctcgg ccgtgacgac gttcagctgc gcggtcccgt tggtgaccag
     7741 ccgcatccgg ttctgcaggt tggtgaacgt gtccagcagg ctgaccagct gctgcaccgc
     7801 agccgcgccg ccgagggcaa gcagggcgcg gtggaggagc gacaccgcgc cctgcgccct
     7861 ctgagccccg ctcccgatgt cctcgatgtt tcgacggacg gttcgggcgc cacgctcagt
     7921 gacgacaatg tcaatacgct cagtggccat ctaccgctcc agcacccgcg ccttgctcgc
     7981 ggcgaccgtc ccgcgctgga cagccttctc cacgaagcct gccggcgcct gggccgagtg
     8041 tccctcgttc agccttccaa tatacgggag attgtttgag atgtatacct cttgaccggt
     8101 ctgtcgtccg gagatgacag tgttgttgat ggcgacctgc cggctgcccg aggggtcggt
     8161 gtcctcggtg gtcgtggtga acggggcgcc cagggaggcg acccagttgg ctcgggctcg
     8221 gcccgtgtca acaggcgttg agacgatgac agcctgcgag acggcgaggg cgacctgccg
     8281 gacggtccgg ctggcgccct cctccacctg ccgacctcgg cgtcggatgc ggcgagcgaa
     8341 gtcagctagg cttggcaccc ttccctcccc gccggctctc tgcctcggcc tcccggtgct
     8401 gatggtactg ctcgtccatc tcccggatgt agtagtgcat gtcctcccgc tggtcgtcgc
     8461 ttacgcccag gcggtcgcag tactcgtcga tcgccgtcca ggggatgtag ccgtcacccc
     8521 agcccccacg acacgtatgc agatcgaaga acgccccgta gtagagctcg agccccatct
     8581 ccagctgcgg ggcgttcctg atcctctccg gcagtggcat gcgctcccgc atgcactgct
     8641 gtatgatgcg acgctcgacc ggtccttgct ccagcgtata gagaaggacc gccctcagtt
     8701 tcccgcgtcg ttctcccgca cggtctgccg gaacagcgca gccttgctgg cctgctcctg
     8761 caggtcggtg aacaggtcgg gcaggttctt gaacgtctca agcacattcg ccacgctgaa
     8821 ctccatgacg gagccgtcgg gcgcctcgat gccctggacc cagtcctcgc cctgccggac
     8881 ctcccagttg agaatgaccg cctcggcgta ggtctcgcgc agcagctgca ggccccgctc
     8941 attgtccatc atctcggtct ggatggcgcg cttgaacggc ttggtcttgg cctccagcac
     9001 ctgggcgaag cggcggttgc cgccgccggc ccgagccagc gtgacgcgga agtcaccata
     9061 gtcgacgacg atcccagcct tctccaggtt gggatcggtc ttgaacagct tgtacattga
     9121 cattgagcgt tgctcctcag cgttgctcgg ttgaagtgga ggggcggccg gttggccgcc
     9181 cgccccttag cctgccaggt cgggcaggta gtcgaagaac acccacatcg cagtgtagtc
     9241 gaggtccggg tcgatcttgg cagcggtcgc cgcgtccatc gacagcggga gctggatggc
     9301 ctggtcctgc tggacgttca gccggccgtc gcccagggcg accagcggga tgtcgatgac
     9361 catgccggag ttggccttca ccagcgccat gtcgatggtg acgtccgcgt tctcgcgaac
     9421 cgccttgacg gcggcgatgt ccgagaagta ggccgtcacg ttgccgccga cctggaaggt
     9481 gccagcggtg acgtcgaagg cgccgagaac cgccactgcc ttgttgggag tgaggttgtt
     9541 gttgatggtg atcgtcagct ccgtgaggaa ggcgaacagc gccaccgggg cctcgtcggc
     9601 ctccaccttg gacatcttca ggcggacgaa gtcgctcgac gtgttgaagg cgtcggcctc
     9661 gacgatcgct ggccggctgc cagccttgag gccctcagct gcctcgcgct gctcgttgtc
     9721 cgtggccatg aaggacacgt ccagggtcag cttgttggcc tgcggcacgt tgatcgagat
     9781 ctcgctgggc accgctccga ccaggtactc ggcctggatc tgattgggca gcgaggtctc
     9841 aggggcgccg agggtccgct cgatgttgta ggaccggcgg atgacgtcgg acccgagctc
     9901 gttcttgagc acccggccga agtacaggcg gatggtcttg ccagcgcccg cgtcggtcgt
     9961 catcgccagg gccgacttgt caaactcgat gcggttggcg gcgatcgagc ggatccgctt
    10021 caggccgttg ttctcggtct cgtcgaacgc ctcagcggcg aggtcgccac cgacgaagac
    10081 ccactcgccg gggatcaggc ccagggtggt aaagtccagg
//
LOCUS       DAOQYE010000004         8574 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_8730_length_8574_cov_3.570607, whole genome shotgun sequence.
ACCESSION   DAOQYE010000004 DAOQYE010000000
VERSION     DAOQYE010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 8574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8574
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8730_length_8574_cov_3.570607"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(718..2820)
                     /locus_tag="PKN52_00330"
     CDS             complement(718..2820)
                     /locus_tag="PKN52_00330"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNQ98408.1"
                     /translation="MRFLKPIIAFALAALVLAACTKPAPPDTTPPAVTLDAPAGGTTV
                     ELGTLVTLAGTASDAGGVAGVSILVDGAVVNTVVPTDEDWTYDWLPAAVGDYAVSAKA
                     TDKAGNSTTTDAIAVSVTAAEVPATTGSVGGTVSRVDLGPAALAASAAVAADAKPAPI
                     EAGQVFVTFKAGARDVTFGANGTATAGAFTFKADGGFSFAGSEFVRERAYPVSSGLSL
                     YRTSGLDEAGTRAVVDRLRTSPLVAEAFPNWILSADAEPDDTYYPIQWSYPQLNLPEA
                     WDSETGDTTHITVAVLDTGRYDHPDVQWAAGGANFANWNGAAPGEGTIDDPHTAPGGS
                     PHGTHVAGTIGAATDNALGVAGVNWNVDVLPVKVLAANGSGSFAGIIEGLFWVSGDDW
                     DGYGGHVNDNIPSVINMSLGGNVETYCPDSLNGIFASLADMGIYTVVSAGNDSSPADV
                     YFPASCPSVITVGATGPTALRASYSNFGPFVDVMAPGGDFYANDPVNGNVLDGIISTV
                     DSNSYDFYQGTSMASPHVAGIVSLMLAQEPDLTLSQVRQRLHNASVPLTLGECSVPTL
                     GLDGMNGCGAGLLDAAAALRGDVLTAPSAFVYAVPNTEGGTVPVFYGDLAALEKLASY
                     KTEAVSDGAGGFTYSLDGLEPGAYQVIALELRDPETGISSIDRIGWAYDVAVEAGAAA
                     EADVSVVPMYLLLPTSPD"
     gene            3077..3325
                     /locus_tag="PKN52_00335"
     CDS             3077..3325
                     /locus_tag="PKN52_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98409.1"
                     /translation="MEYQSEPTLADLLALLRRGLVFALAVAVGAGLLTYFLSKRTTPE
                     YDSTTTLLASKPGNTQGTFGVSLVGSVKCAFAIIRSAS"
     gene            complement(3385..4566)
                     /locus_tag="PKN52_00340"
     CDS             complement(3385..4566)
                     /locus_tag="PKN52_00340"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNQ98410.1"
                     /translation="MTLDARTVFVMAVGFLAVTTITLGLLVRTLPADTRRGARVGTLA
                     TATLGVSWALIALEGLVPEALSLLGGNLLYLIAAALVYQSIRLLDGEKATGWVYACVV
                     APAILATALARYVVDAYSVRVIVMSVALAFLLTLAARRLFVAPRGVLFNPGRRAAAYW
                     MATSAALLVMRVVLTLAQGGAPPLLGDEALPNLFVAVSVVIALGAVFAYFLLFSGRVT
                     AELAVQAHLDPLTELLNRRGFEERAKQELKRAARNGSPVSLLMVDANEFKNINDSWGH
                     QVGDEALRAIANGIRARVRPYDLIARLGGDEFVVLLPGLDAQSAAALIPRIRESIANQ
                     PTPHKGRLDVSIGRASLRPRASGRGRVEASGEEVDAADVLRRLIAAADGDLYGVKHTR
                     F"
     gene            complement(4855..5829)
                     /locus_tag="PKN52_00345"
     CDS             complement(4855..5829)
                     /locus_tag="PKN52_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98411.1"
                     /translation="MKYKHCHGRIGSAGGQSALSGASQEIAAELARHEFPSLEAAQKH
                     LDEYVRRRNRTPLPEFNGLSPEEMAQLLYAPFDSPGVIVIPVVLDSEPTAEILDLFMF
                     MAESLAGGGIKLTEKGNLPRALCRAAAERFAAAGSAGPFYLDGSMPNRESDLPALRVT
                     RKVAELAGLVRKYRGHLELTRKAKRLVQEHGARGVYPALFRAFVEDYNWAYMDGYPDL
                     DLVQTSWAFTAYLLMRFGGETRSAGFYAEQFLNAFPAVTAEVEESPYEEPRKVGARCY
                     QLRALERFAEFMGLARVGYDRPTRHFAELTVTMLPLLGEVVRFAGRSP"
     gene            5982..6728
                     /locus_tag="PKN52_00350"
     CDS             5982..6728
                     /locus_tag="PKN52_00350"
                     /inference="COORDINATES: protein motif:HMM:NF015686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HNQ98412.1"
                     /translation="MEWLTSPAAWVALASLTVLEIVLGIDNIVFISILAGKLPAEQRD
                     RARVVGLALAMLTRIALLFSISLLSHLTAPLFTVFGMQITGRDLVFILGGMFLIFKAT
                     QEIHERLEGPRHAQQSAGPAAATFAGVITQIALLDVVFSLDSVITAVGMADHLAIMIA
                     AVVIAVGVMMLSVSSVAGFVEQHPTVKMLALSFLLLIGTTLVAEGLGQHVPKGYVYFA
                     MGFSVMVELFNMRARAVREPVTLYERGPKA"
     gene            complement(6959..7684)
                     /locus_tag="PKN52_00355"
     CDS             complement(6959..7684)
                     /locus_tag="PKN52_00355"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNQ98413.1"
                     /translation="MRHTVGSGREEDAFARTTAEWVVERLKDDILTGRLAPGTKLVER
                     DLAERYGVSRTPLREALNQLVARQLATTVPYRGVFVRKVDLGFARDVYEVRSALEGLA
                     GKLAAERATKDEIRLVEAHFKSIERLSRLQASDQSVRDDIMRNNTRFHRAIAVSAHNA
                     LLLNKIEEIWTSVSLVRFYVWQTEDRIESSLLEHAEMLEALRKRDGERMQVLCAEHAE
                     KAWRHVDRVLRGGEATRPNGLVG"
     gene            complement(7789..>8574)
                     /locus_tag="PKN52_00360"
     CDS             complement(7789..>8574)
                     /locus_tag="PKN52_00360"
                     /inference="COORDINATES: protein motif:HMM:NF040249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98414.1"
                     /translation="SGLASWRRDLEHVEKAFVFAGMPVRAGTSAVDMVLNGFTGVADV
                     FDGPTNFMGAFSPEGAPGELTRGLGTDYEILRTNIKRWPVGSPIQAAVDSLTALMAEH
                     DLAPERIGRLTVRLPTGGAETVDDRHMPDINLQHIMAVTLLDGGLVFTASQNYARMSV
                     PDVVELKKRIRLIADASLDNTDPPRQGIVEIELTDGTSLRHHTRAVRGTYDNPMPEDE
                     VVRKAQDLLVPLLGEQRCAELVRAVLDLPNLDDVQRLRPLLTL"
BASE COUNT         1387 a         2908 c         2874 g         1405 t
ORIGIN      
        1 gcgcgacgtc ggcaccccca gcccccgcct gcgccaaggc gccgcccccg aagacgagcg
       61 cgccgcaggc cacgaccatc agggccatga ctaccaggcg ccaggccgac cggcttcccc
      121 tattccctac gctctccatc ccaagacggt aacagaaccg caccagctcg aacctgggtg
      181 gccagccatg ttctccgtgg gcgcgtcggc gacggggttg acgatcctga cgcagcactg
      241 ctgtggggct tcaacgctca tgcgcctcgt ctcgtgagcc tgtcggccgg ccggcggttc
      301 cgaggtcgag accttgctcc agcagagcta tgcagtctcg ctccgcagag cggtagccgg
      361 cctcgccgtc caccaaggct tcgagtgcac cccggacaag ggctgatacg gacgtgtggc
      421 gcctggccgc gatcgacctg acgtcctcga gcgtgttact ggcgagagac actgtgacgt
      481 tgcgacgcgc catggtaggc ccccttcacg ttgccgacac ttgccagcgt aagacctact
      541 atgcgtaaag catgtggggc ggcaccgatg gcggccacgg ggaagggcct cacaggctga
      601 acgctcccat tacgggcaat gagcgatcaa agtagcggcg ccgacctcgc gtggtcggcg
      661 ccgccaatcg tcgttcggtt cgttcggggg cctagaggcg cgccgcgaac cctcggctca
      721 gtccgggctc gtcggcagca ggaggtacat gggcacgacg ctcacgtccg cctcggcggc
      781 ggcaccggct tccacggcca cgtcgtaggc ccagccgatg cggtcgatgc tcgagatgcc
      841 ggtctccggg tcgcgcagct cgagcgcgat gacctggtag gcgcccggct cgaggccgtc
      901 gagcgagtac gtgaagccgc ccgcgccgtc ggagacggct tcagtcttgt agctggccag
      961 cttctcgagg gccgcgaggt cgccgtagaa caccggcacc gtgccgcctt ccgtgttcgg
     1021 cacggcgtag acgaaggcgc tcggcgcagt gaggacgtcg ccgcgcaggg ccgccgccgc
     1081 atcgaggagg ccggcgccgc acccgttcat gccgtccagg cccagggtgg gcacgctgca
     1141 ctcgccgagg gtgaggggca cggaagcgtt gtgcaggcgc tggcgcacct gggagagcgt
     1201 gaggtcgggc tcctgggcaa gcatgagcga gacgatgccc gcgacgtgcg gggaggccat
     1261 gctcgtgccc tggtagaagt cgtagctgtt cgaatcgacc gtgctgatga tgccgtcaag
     1321 gacgttgccg ttgaccgggt cgttggcgta gaagtcgccg cccggcgcca tcacgtcgac
     1381 gaacggcccg aagttggagt acgaggcgcg cagcgcggtg gggccggtag cgccgacggt
     1441 gatgacgctc gggcagctgg ccgggaagta gacgtccgcc ggcgaggagt cgttgccggc
     1501 cgagacgacc gtgtagatgc ccatgtcggc caggctcgcg aagatcccgt tcaggctgtc
     1561 ggggcagtag gtctcgacgt tgccgccgag gctcatgttg atgacgctgg ggatgttgtc
     1621 gttaacgtgg ccgccgtagc cgtcccagtc gtcgccgctc acccagaaca gcccctcgat
     1681 gatcccggcg aagctgccac tgccgttggc cgccaggacc ttgacgggca gcacgtcgac
     1741 gttccagttc acacctgcga cgcctagagc gttgtcggtg gcggcgccga tggtgccggc
     1801 cacgtgcgtg ccgtgcggcg agccgcccgg agcagtatgg gggtcatcga tcgtgccctc
     1861 gccgggcgcc gcgccgttcc agttggcgaa gttggcgccg ccggcggccc actgcacgtc
     1921 ggggtggtcg tagcggcccg tgtctagcac ggccacggtg atgtgggtcg tgtcgcccgt
     1981 ctcgctgtcc cacgcttccg gcaggttgag ctgcgggtag gaccactgga tcgggtagta
     2041 agtgtcgtcc ggctcggcgt cggccgagag aatccagttc gggaacgcct cggcgacgag
     2101 cggcgacgtg cggagtcggt cgaccacggc cctggtgccg gcttcgtcga gcccgctggt
     2161 gcggtacagc gagaggccgc tgctcaccgg gtaggcgcgt tcgcggacga actcgctgcc
     2221 ggcgaacgag aagccgccgt cggccttgaa ggtgaaggcg ccggccgtcg ccgtgccgtt
     2281 ggcgccgaag gttacgtcgc gcgcgcctgc cttgaaggtg acgaacacct ggccggcctc
     2341 gatgggagcc ggcttggcgt ctgccgcgac cgcggcggac gccgccagcg cggccggtcc
     2401 gaggtcgacg cggctgacgg taccgcccac ggagccggtg gtcgcgggca cctcggccgc
     2461 cgtgacgctc acggcgatgg cgtcggtcgt ggtgctgttg ccggccttgt cggtcgcctt
     2521 ggcgctgacg gcgtaatcgc cgacggcggc gggcagccag tcgtaggtcc agtcctcgtc
     2581 cgtcggaacg acggtgttga caacggcgcc gtccaccagg atgctcaccc cggcgacgcc
     2641 cccggcgtcg gaggcggtgc cggccagcgt aacgagcgtg ccgagctcga cggtcgtacc
     2701 gcccgccggc gcatcgagcg tgacggccgg cggcgtagtg tccggcggcg caggcttcgt
     2761 gcaggcggcc agcacgagcg cagccaaggc gaacgcgatg attggcttca ggaacctcat
     2821 cttctctcct cacttctcgc agcgaaaggg ctgtggggcg ggcacggcga tctaggcgac
     2881 tgcgcgtcca gcacggcgaa atgcccgcct tggccgaggc caggaaagct gcggttcaaa
     2941 caatcctagc atcggcgcta ggttatgctc tgccaacatc caccgggcag gggcctcgcg
     3001 agaggtggag tcccgtcggg gtgcataaga ggaggggacc ggtctcccgc cccggcgcgg
     3061 aagaccgaga gactagatgg agtaccagtc cgaaccgacc ctggcagacc tgctagcgct
     3121 cttgcgccgc ggcctggtgt tcgcactagc cgtggccgtg ggcgccggcc tcctcaccta
     3181 cttcctaagc aagcgcacga cacccgagta cgactcgacc accacgctgt tggccagcaa
     3241 gccgggcaac acacagggca cgttcggggt gtcgttggtc ggctcggtga agtgcgcctt
     3301 cgcgatcatc cggtcggcat cgtaaccgac tgccgcagga gcgcgagaac ccttacccgg
     3361 tgctcggaaa ctaccggccc caccctagaa ccgcgtgtgc ttcacgccgt acaggtcccc
     3421 atcggcagcg gcgatgaggc gccgcaacac gtccgccgcg tccacttcct cgcctgacgc
     3481 ctccacgcgg ccgcgccccg atgcacgcgg ccgcaggctg gctcgcccga tactgacatc
     3541 caagcgcccc ttgtgcgggg tcggttggtt ggcgatcgac tcccgtatgc gcgggatgag
     3601 tgcggcggcc gactgcgcgt cgagcccggg gagcaggacc acgaactcgt cgccgcccag
     3661 gcgcgctatc aggtcgtacg gtcggacgcg cgctcggata ccgttcgcga tggcgcgcag
     3721 cgcctcgtcg cccacctggt gaccccagct gtcgttgatg ttcttgaact cgttcgcgtc
     3781 caccatcagg aggctgaccg gcgaaccgtt gcgtgccgcc cgtttcagct cctgcttcgc
     3841 gcgctcctcg aaaccacgac ggttgagcag ttccgtgaga ggatccaggt gcgcctgcac
     3901 cgccagttcc gccgtgacgc gaccactgaa caggaggaag taggcaaaca ctgcccccag
     3961 ggcgatgacc acgctgacag cgacgaacag gttgggaagg gcttcgtcgc cgagcagagg
     4021 cggtgcgccg ccttgggcca aggtaaggac cacccgcatg acgagcagcg cagcggacgt
     4081 ggccatccag taggcggcgg ctcgcctccc cgggttgaag agcacgccac gaggcgccac
     4141 gaacaagcgt cgcgcggcca acgtgagcag gaacgccaac gccaccgaca tcacgatcac
     4201 gcgcacggaa tacgcatcca cgacgtaccg cgccagcgca gtagccagga tggcgggagc
     4261 gacgacgcaa gcgtagaccc agccggtcgc cttctcgccg tccaggagcc ggatactctg
     4321 atacacaagc gccgcggcga tcaggtacag caggttgccg cccagcaacg acagcgcttc
     4381 gggcaccaaa ccctcgagcg cgatgagcgc ccaggagacc ccgagtgtgg cggtggccaa
     4441 agtgccaacg cgtgcgccgc gcctggtatc ggccgggagg gtgcgcacca gcaggcccaa
     4501 tgtgatcgtc gtgaccgcca ggaagccgac ggccatgacg aacaccgtgc gggcgtcaag
     4561 ggtcatggtg gacccgcctc ggggatgtta ggtcgtcgag tgtcggcatg ctctggggag
     4621 tataggcgaa cttcttcggg cacaacctgt ccggtgttca agagtgccgg ctgccagcct
     4681 aaggtttcaa aggcctgcga ccaagtacag ggtctcccga cgttcgaggg ccgctccgga
     4741 aagttcccgc atgctcactg caatccgcat gttcacgatg tcgtgaacat aggacgttca
     4801 gtgaacctca cgtggtcgtc cacccctatc cacgacgcgg gaagaagccg cctgtcacgg
     4861 cgaacgcccg gcgaacctaa cgacctcgcc caacagcggc agcatcgtga ccgtcaactc
     4921 ggcgaagtgc cgagtcggtc ggtcataccc gacgcgcgcg aggcccatga actccgcgaa
     4981 ccgctccagg gccctgagct ggtaacagcg ggcgccgacc ttccgcggct cctcgtatgg
     5041 gctctcttcc acctcggccg tcaccgcggg gaaggcgttc aggaactgct cggcgtagaa
     5101 ccctgccgat ctggtctcgc ctccgaagcg catgaggagg taggccgtga aggcccatga
     5161 agtctgcacc aggtcgaggt ccgggtagcc gtccatgtag gcccagttgt agtcctcgac
     5221 gaaggcccgg aacagggccg ggtagacacc ccgcgcgccg tgttcctgga caagacgctt
     5281 ggcctttcga gtgagctcga ggtggccgcg atacttcctg accaacccgg ccagctccgc
     5341 gaccttgcgc gtgacgcgca acgcaggaag gtcgctctcc ctgttgggca tcgagccgtc
     5401 aaggtagaac gggccggccg agccggcggc ggcgaaccgc tccgcagcgg cgcggcacaa
     5461 ggctcgtggc aggttgcctt tctcagtcag cttgataccg ccgccggcca ggctctcggc
     5521 catgaacatg aagaggtcca agatctcggc ggtgggttcg gaatccagca ccaccggtat
     5581 gacgattacg ccgggcgagt cgaaaggtgc gtagaggagt tgcgccatct cctctggaga
     5641 gagcccgttg aactccggga gcggcgtgcg gttgcgtctg cgcacgtact catcgagatg
     5701 cttctgggca gcctccagag aagggaactc atggcgcgcc agctcagccg cgatctcctg
     5761 gctggcgccg ctcagtgcgc tctggccgcc ggcgcttccg atccggccgt ggcagtgctt
     5821 gtacttcatg ccgctgccac aagggcacgg gtcgttacgt ccgatcggag gcatggctaa
     5881 cggtagcgcg tgcgagtcgc cgacagttgc cgcggtccgc ggtccgcggt tcgcgagcca
     5941 cccccttgtg ccgcaggccg cgttcctcca caatcacctg cgtggaatgg ctcaccagtc
     6001 cggccgcttg ggtggctcta gcctccctca ccgtcctcga gatcgtcctc gggatcgaca
     6061 acatcgtctt catctccata ctcgccggca agctgccggc cgagcagcga gaccgtgccc
     6121 gcgtggtggg cctcgcgctg gccatgctca cgcgcatcgc gctcctcttc tccatcagcc
     6181 tcctgtctca cctcacagcg ccgctgttca cggtcttcgg catgcagatc accggccgcg
     6241 acctcgtctt catcctcggc ggtatgttcc tgatcttcaa agccacgcag gagatccacg
     6301 agcgcctcga agggccaagg cacgcgcaac agtccgccgg gccagctgcg gccaccttcg
     6361 ccggcgtcat cacccagatc gcgctgctcg acgtggtctt ctcgctcgac tccgtcatca
     6421 ccgcggtcgg catggccgac cacttggcca tcatgatcgc ggccgtcgtc atcgccgtcg
     6481 gcgtgatgat gctgtcggta agctcggtgg ccggcttcgt ggagcagcac cccaccgtca
     6541 agatgctcgc cctctccttc ctcctcctga tcggcaccac cctcgtggcc gaggggctcg
     6601 gacagcacgt gcccaagggt tacgtctact tcgctatggg cttcagcgtg atggtcgaac
     6661 tcttcaacat gcgggctcgg gccgtgcggg agccggtgac gttgtatgag cgcgggccga
     6721 aagcgtaacg gacgcgcgac gcgtcaagaa cgctcgcgcc aagcccctct tggaggcacg
     6781 ctggtcgagt cggaaggcaa agctagtccc gagatggtgg tcgcacgggc gcgcgagcgg
     6841 gtgcatttga ccgatagtga gttgccggca gaaaccatct tggcaggcag ggacgccgac
     6901 cgcagatagg gccgtccccc gtccgcatcg ataaaaggtg cccggcctta gggttagctc
     6961 aaccaaccag tccgttaggc cgagtggctt cgccaccccg caacacccta tccacatgcc
     7021 gccaggcctt ctccgcatgc tcggcgcata ggacttgcat ccgctcccca tcgcgcttac
     7081 gcagcgcctc cagcatctcg gcgtgctcga gcaagctgct ctcgatgcgg tcttccgtct
     7141 gccagacgta gaagcgcacg aggctgacgc tcgtccatat ctcctcgatc ttgttgagga
     7201 gcaaggcgtt gtgcgcagac accgcgatgg cccggtggaa ccgcgtgttg ttcctcatga
     7261 tgtcgtccct gacgctctga tccgacgcct gcaggcgcga cagcctttcg atgctcttga
     7321 agtgcgcctc caccaaacgg atctcgtcct tggtcgcccg ctctgccgcg agcttgccgg
     7381 ccaagccctc gagggccgac ctcacctcgt agacgtcgcg cgcgaagccc aggtcgacct
     7441 tgcgaacgaa gacaccacgg tacgggacgg tggttgcgag ttggcgcgcc acgagctggt
     7501 tcagggcctc ccgcagcggc gtcctgctga cgccgtagcg ctcggccaaa tcgcgctcaa
     7561 cgagcttcgt gccgggcgcc aggcgcccgg tgaggatgtc gtccttgagc cgttcgacga
     7621 cccactcagc cgtcgtgcgg gcaaacgcgt cctcttcgcg accggaaccg accgtatgtc
     7681 tcactcgcca gtcaggttag catcgcgctt cccgcgagca ttcgccagga agcgcgcggc
     7741 cgtgaccaac gacgatcgcc gtggcgagcc gcgattcgta gtcgcgggtc atagcgtaag
     7801 gagcggccgg aggcgctgga catcgtccag gttcggcagg tcgagtacgg cgcgcaccag
     7861 ctccgcgcac ctctgctcgc cgaggagcgg tactagtagg tcttgcgcct tcctcaccac
     7921 ctcgtcctcc ggcatggggt tgtcgtaagt gccgcgcacg gcgcgcgtgt ggtgcctaag
     7981 gctcgtgccg tccgtgagtt ctatctcgac gatcccctgg cgtggcggat ccgtgttgtc
     8041 gaggctcgca tcggcgatca gccggatgcg cttcttgagc tccaccacgt cgggcacgga
     8101 catcctcgcg tagttctgcg aggcggtgaa gactaggccg ccgtccagga gggtcacggc
     8161 catgatgtgc tgcaggttga tgtctggcat atgccggtca tccaccgtct cggcgccgcc
     8221 ggtcggcagc ctcacggtga ggcgcccgat gcgctccgga gccaggtcgt gctcggccat
     8281 gagcgcggtc aacgagtcga cggcggcctg gatcggcgag ccgaccggcc agcgcttgat
     8341 gttcgtgcgc aggatctcgt agtccgtccc gagcccgcgc gtcagctcgc ccggcgcgcc
     8401 ttccggcgag aacgcgccca tgaagttggt cggcccatcg aacacgtcgg ccacgccggt
     8461 gaagccgttg agcaccatgt cgacggcgct ggtgcccgcg cgcacgggca tgccggcgaa
     8521 cacgaacgcc ttctcgacgt gctcgaggtc cctgcgccag ctcgcgaggc cgga
//
LOCUS       DAOQYE010000005         8451 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_8875_length_8451_cov_3.163649, whole genome shotgun sequence.
ACCESSION   DAOQYE010000005 DAOQYE010000000
VERSION     DAOQYE010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 8451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8451
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8875_length_8451_cov_3.163649"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..655)
                     /locus_tag="PKN52_00365"
     CDS             complement(<1..655)
                     /locus_tag="PKN52_00365"
                     /inference="COORDINATES: protein motif:HMM:NF015558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HNQ98415.1"
                     /translation="MTASVVSGRLRSLLPGLFLVVLLTFVAYELARVPGLRLLGPLVL
                     ALLAGALWRLARGGRVVGVMPGARFAAKVLLRLGIVLLGVRLDMRALAVVGPTVLVGS
                     ALGVLVAFGAVELVGRRWGVPKDLRRSLAVGTGVCGASAIAAALPVLKAEERHASLSV
                     AVVSVLGTLGVLAFAAWDGLALVSSRLVAAVAGATLQEVGHVVAAGAAVGGGEGDLAL
                     "
     gene            737..1636
                     /locus_tag="PKN52_00370"
     CDS             737..1636
                     /locus_tag="PKN52_00370"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNQ98416.1"
                     /translation="MPLEPHLLRVFLVVARGGSISEAARRLHRSQPAVTAAVKRLEAH
                     FGEPLFGRGGKGVRLTDLGARLLPHAEALERVLDGVNQLAAEASGLEGARLRIAASTT
                     IALYWLPTRLARFCSDNPSATLVVHTRNSREAVEELTAGEVDLALVEAPAASWAGLPS
                     GLLVATPVHADELVLVVDPRHALAGADHVVPEELDGLAFVGREAGSGTRAVLEQALAT
                     VGASPAVRLELGEPEAIKRAVRAGLGAAVLSRVAVAQEVERGELVALSIAHPGFRRQF
                     TLLHPPEALASHACWTFRTLAVA"
     gene            complement(1633..3348)
                     /locus_tag="PKN52_00375"
     CDS             complement(1633..3348)
                     /locus_tag="PKN52_00375"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HNQ98417.1"
                     /translation="MPTTDEATRHRKFVPLPIDPLELVARAKALLEEPVSAETMPAFV
                     AAVNEVSVDFSELYASRMRANDEDTADEAAKQSYLDFLTNHVPELAGLGAALSRKQLE
                     VADYTPPADLADHWADMRVDVELFREENLPLEAEVASLAQAHSEVSGSLRVELDGETL
                     TLGQAGAKLESPDRDLRERAYRAMEAGREATRPRLDEIFAQMLPLRRRIALNAGLPDY
                     RAYAWRKAKRREYTPEDVLAMHDSVAKEVVPRLRAVYERRARLLGLERLRPWDLDCDV
                     HGRDPLAPFQTVDELVEGMARMYSALDPELAGAFDLLRDGWMDLEPRQTKVPGLGYAN
                     YFPRSKRPYIYWSAVGTDDDVLTMRHEGGHAFHALLTQNRWPYLAHMSERPEMCELAS
                     QAMELLTLPYLAREAGGFYSPEDAGRSQAALLVRALSLLVSASRVDAIQHFIYTHPSE
                     TGPTIAEIDAQWRRLGERFDLGMDMTGFDRQLSKGWHIIHVFHYPFYYIEYAMAYLGA
                     LQVWRNAMQDAPRALRQYKEALALGGTRPLDELYEVAGIRFAFDRSTVAGLSQLAFDA
                     LGDEA"
     gene            complement(3409..4536)
                     /locus_tag="PKN52_00380"
     CDS             complement(3409..4536)
                     /locus_tag="PKN52_00380"
                     /inference="COORDINATES: protein motif:HMM:NF015535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HNQ98418.1"
                     /translation="MSVSSSVAGRRRVRELGIVPGVLAPGPHNAITDVAGVRVGHETL
                     YEGEGIRTGATVVLAHLDDPYRWRVPAAIFVGNGYGKLIGATQVRELGELETPIALTN
                     TLCVARAAEALVDWTLGRKGNAGVRSVNPFVGETNDGYLNDIRARAVRPEHVLAALDS
                     AAEGPVALGAVGAGTGTMAFGFKGGIGSSSRRLPAALGGFTVGALVQSNFGGVLQVAG
                     RPVGVLSGRYYLRDELTGADASAGGAQADASAGGAEAADGSIMIVIATDAPLSSASLG
                     RLAFRSMAGLARTGAAFSNGSGDYAVAFSTAEAVRRRAGGAPRSQGREVGNDDLSPLF
                     LAAIEATEEAIYDSLCMAVRVVGHEGHVGEALPFGLLDESQ"
     gene            4665..5966
                     /locus_tag="PKN52_00385"
     CDS             4665..5966
                     /locus_tag="PKN52_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98419.1"
                     /translation="MSDQQSTPATPAIVRGTPLKYLDKALGGLRDLGLLPENTTKEEA
                     PIVALLSQITDLDQERVVAITRTLAQASLFNDVVRKQVEAMEVGSRYEDVTKSFNSIR
                     DDAKAMVDQLEDGKISTFERLSNIWMKVTRGDISSRFDKIKHTYLEVTKDAQDQIQRE
                     HIILEAYRDFRGALKESEVLAFEVLKKGEAQLETAKQGVNEAMAAVDAYAGEDPAERA
                     RLELARDERVRALQEVDKRYQIAKDLSDNLTISYNTSEVVMARLMQTNTAKERVRAQA
                     VSFFSTNETVLTALTATFTGLFGLNESTRTLEAMTKGVSDSLDTLSDIGGKVQEAALK
                     AGYGPTVRAESVKRLVESVVNYQERSLEIIDEMRKQATTNAAEIRDAVEDGKRRLARL
                     AEQGAKLDIIDAPRSATQGGAGQLDSAEQVGGAERVGGDVG"
     gene            5959..6870
                     /locus_tag="PKN52_00390"
     CDS             5959..6870
                     /locus_tag="PKN52_00390"
                     /inference="COORDINATES: protein motif:HMM:NF039746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6384 family protein"
                     /protein_id="HNQ98420.1"
                     /translation="MADAAASAGPQPAPPPLDDVMLAMDVVDTLRHRERLVEAELASE
                     GRDEAMLESLRNIYASQGIAVSDEVLRQGVAALREGRFVYSPPARGPGTRWAYAYVNR
                     GAWGKLLLALLVVAAVLVFGYDSLVRAPHRNLVRDVGKVYAEVVARSQDAAANQQADT
                     LYGVATGALAKGDDRTARTTLASLKTLQETLNSSYVLRIATDTTGVWRVPDLNQDAAN
                     YYIIVEPVDRNGNNVAVTVTNEETGRSETVKRFGLRVSEATFEAIKADKLDDGIIQND
                     VFGEKSAGHLQPVYRFDTTGAAITSWD"
     gene            6870..>8451
                     /locus_tag="PKN52_00395"
     CDS             6870..>8451
                     /locus_tag="PKN52_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98421.1"
                     /translation="MISGRDTLNTLLSTIAEEQQRMRQVDASLSTNNDELLKLDSSRM
                     QELQRLARMRLNYLANDKTRVEDTSDQQLLKFIAQRDSAYGALQQRVSELEEKSKALE
                     AERAGAMANLDAAAKAIEDAEVATQRRLESDAGYQAKLEATREADRVAGRADAKATQS
                     ESEQATKGEAYRADPLFSYLWRRKYGTAEYRSGGFIFAPLIRYLDGRVARLVGYQDAR
                     ANYNRLTEIPLRLREHATNARAAADAAHAELERLDVEARKADGIPALEDKKAAVEAGI
                     AKLDAAIQEASERHAAAMAESEKFAKGADENYLNAVEFMRTQLEAAPLDVLRVRALAT
                     PSPEDDLVVSRLAGFAKRREELERTVAELRAGAEASHKRAQELDWLRTNYTRSGFDAP
                     NTAFRDAGAVKTGLTQYLTGLITMDALWRLLTQQRTAVPTRVDPTFGSGGFGRGTVWG
                     GGTTPPPVNPGPVIDVIGSVLEGIFGAGGGVGGSSRGSSSRGGSGGFGGGFGGSSRGG
                     SGGFGGGSKGGGSRPSGGS"
BASE COUNT         1394 a         3049 c         2896 g         1112 t
ORIGIN      
        1 gcagggcaag gtccccctcg ccgccaccga cggccgcgcc ggcggccacg acgtggccga
       61 cctcctgcag cgtcgccccg gcgacggcgg ctacgagccg cgacgatacg agcgccaggc
      121 cgtcccaagc ggcgaacgcc agcaccccga gcgtgccgag gacgctcacc acggcgaccg
      181 agagcgacgc gtggcgctcc tcggccttga ggaccggcaa agccgcggcg atcgccgaag
      241 cgccgcacac gcccgtcccg accgcgagcg agcgtcgcag gtccttcggc acgccccacc
      301 tgcgcccgac gagctcaacc gccccgaacg cgacgagcac gccgagcgcg ctgccgacga
      361 gcacggtagg accgacgact gcgagtgcgc gcatgtccag ccgcaccccc agcagcacga
      421 tgcctaggcg tagcaagacc ttggcggcga agcgagcgcc gggcatgacg ccgacgacgc
      481 gaccgccgcg cgccaaccgc cagagcgccc cggccaggag cgcgagcacc agcggtccca
      541 ggagcctgag gccgggcaca cgagcgagct cgtaggcgac gaacgtgagc aggacgacga
      601 ggaagagacc gggcaggagc gaccggaggc ggccggagac gacggaagcg gtcaccccgg
      661 aagcctatgg ccgcggcggc cataacggta gagccgtcat gctgatagag ccataagcat
      721 gagttatggt ctgatcgtgc ccctcgaacc gcacctcctg cgcgtcttcc tggtcgtcgc
      781 gcgcgggggc agcatcagcg aggccgcgcg ccggctccac cgctcgcaac ccgcggtcac
      841 ggccgccgtc aagcgcctgg aggcgcactt cggcgagccg ctcttcgggc gcggcggcaa
      901 gggcgtgcgc ctcaccgacc ttggcgccag gctcctgccc catgccgagg cgctggaacg
      961 cgttctggac ggtgtcaacc agctagccgc cgaggcgtcg ggcttggagg gggcgcgact
     1021 gcgcatcgct gccagcacca ccatcgcgct ctactggctg ccgacccgcc tggcgcgctt
     1081 ctgctcagac aacccgagcg ccaccctggt cgtgcacacg cgcaactcgc gcgaggccgt
     1141 ggaggagttg acggcgggtg aggtcgacct ggcgctcgtc gaggcgcccg ccgccagttg
     1201 ggccggtctc ccgagcgggc tgcttgtcgc caccccggtt cacgctgacg agctcgtgct
     1261 ggtcgtcgac ccccgccacg cgctcgccgg tgccgaccat gtcgtccccg aggagctgga
     1321 cggcctcgcg ttcgtcgggc gcgaggccgg ctccggtacg cgcgccgtcc tggagcaggc
     1381 cctcgcgacc gtgggcgcct ctccggccgt gcggctcgag ctcggcgagc cggaggccat
     1441 caagcgcgcg gtgcgcgccg gcctcggggc ggcggtcctc tcgcgcgtcg ccgtcgccca
     1501 ggaggtcgag cgcggcgagc tcgtcgccct ctccatcgcg caccccggct tccgccgcca
     1561 gttcacgctc ctccacccgc cggaggcgct cgccagccat gcctgctgga cgttcaggac
     1621 gctggcggtg gcctaggcct cgtcccccaa cgcgtcgaag gcgagctggc tgaggccggc
     1681 gacggtcgac cggtcgaagg cgaaccggat gccggcgacc tcgtagagct cgtcgagcgg
     1741 ccgcgtgccg ccgagcgcca gcgcctcctt gtactgccgc agggcacgtg gcgcgtcctg
     1801 catggcgttg cgccatacct gcagcgcccc caggtaggcc atcgcgtact cgatgtaata
     1861 gaaggggtag tggaagacgt ggatgatgtg ccagcccttg gagagctggc ggtcgaagcc
     1921 ggtcatgtcc atcccgaggt cgaaccgctc cccgagcctc cgccactgcg cgtcgatctc
     1981 ggcgatcgtc ggacccgtct cgctcgggtg cgtgtagatg aagtgctgga tggcatcgac
     2041 cctcgaagcg ctaaccagca gcgagagcgc gcgtacgagt agcgcggcct gcgagcgacc
     2101 ggcgtcctcc ggcgagtaga agccgccggc ttcccgcgcc aggtacggca gggtgagcag
     2161 ctccatcgcc tgcgaggcga gctcgcacat ctccggccgc tccgacatgt gggcgaggta
     2221 cggccaccgg ttctgcgtca gaagggcgtg gaaagcgtga cccccctcat ggcgcatggt
     2281 gaggacgtca tcatccgtcc cgacggcgct ccagtagatg tacggacgct tcgaacgcgg
     2341 gaagtagtta gcgtacccga gcccggggac cttggtctgc cgcggctcga gatccatcca
     2401 accgtcccgc aggaggtcga aggcgcctgc cagctcggga tcgagcgcgg agtacatgcg
     2461 cgccatcccc tctaccagct catccacggt ctggaagggc gccaacgggt cgcggccgtg
     2521 cacgtcgcag tcgaggtccc agggccgcag gcgctccagg cctagaaggc gggcgcggcg
     2581 ctcgtatacc gcgcgcaagc ggggcacgac ctccttggcc accgagtcgt gcatcgccag
     2641 cacgtcctcc ggcgtgtact cccggcgttt cgccttgcgc cacgcgtacg cgcggtagtc
     2701 gggcagcccg gcgttgagcg cgatgcgcct gcggagcggc agcatctggg cgaagatctc
     2761 gtccaagcgg ggccttgtcg cctcgcgacc ggcctccatg gcgcggtagg cgcgttcccg
     2821 caggtcgcgg tcgggcgact cgagcttggc gcccgcctgc ccaagcgtga gcgtctcgcc
     2881 gtcgagctcg acgcgtaggc tgccgctcac ctcgctatgc gcctgcgcca gggaggccac
     2941 ctcggcctcg agcgggaggt tctcctcacg gaacaactcc acgtcgaccc gcatgtcggc
     3001 ccaatggtcg gccaggtcgg ccggaggggt gtagtcggcc acctcgagtt gcttgcgaga
     3061 cagggccgcg ccgaggcccg ccaactcggg cacgtggttc gtcaggaagt ccaggtaaga
     3121 ctgcttcgcg gcctcgtccg cggtgtcctc gtcgttcgcg cgcatccggc tcgcgtacag
     3181 ctcggagaag tcgacgctca cctcgttcac cgcggcgacg aaggcgggca tggtctccgc
     3241 cgagaccggc tcttccagca gcgccttggc cctggcgacc agttcgagcg ggtcgatcgg
     3301 taacgggacg aacttgcgat gtcgagtggc ttcgtcggtc gtcggcatgg cgccagtcta
     3361 gccagcgcgg tcgctaaagc cggtgcgaac gcagcatgcg gccagccatc actggctctc
     3421 gtccagcagg ccgaacggca gcgcttcccc cacgtgaccc tcatgcccga cgacgcgcac
     3481 tgccatgcac agcgagtcgt agatggcttc ctccgtcgcc tcgatggccg cgaggaagag
     3541 gggcgagagg tcgtcgttgc cgacctctcg cccctgggac ctcggagccc cgccggctcg
     3601 gcgcctgacc gcctccgccg tcgagaacgc gacggcgtag tctcccgaac cgttggagaa
     3661 ggcggcgccc gtgcgcgcca ggccggccat cgaccggaac gccaaccggc caaggctcgc
     3721 cgaactcagg ggggcgtccg tggcgatcac gatcatgatc gagccgtcgg cagcctccgc
     3781 gccgcccgcg gacgcgtcag cctgcgcgcc gcccgctgac gcgtcggcgc cggtcaactc
     3841 gtcgcggagg taataccttc cggacagcac gccaacgggc cgccccgcca cctgcagcac
     3901 gccaccgaag ttcgactgca cgagggcacc gaccgtgaaa cctccgagcg ctgccggcag
     3961 ccgccgtgac gagctgccga tgccgccctt gaacccgaac gccatggtgc cggtgccggc
     4021 cccaacggca ccgagcgcca ccggcccctc ggccgcggag tcgagggcgg ccagcacgtg
     4081 ctcgggtcgc acggcgcggg ccctgatgtc gttgagatag ccgtcgttcg tctcgccgac
     4141 gaacgggttg acggagcgca cgccggcgtt gcctttacgg ccgagcgtcc agtcgacgag
     4201 cgcctcggcc gctcgcgcta cgcacagggt gttcgtgagg gcgatggggg tctccagctc
     4261 gccgagttcc cgcacctgcg tggcgccgat cagcttgccg tagccgttgc cgacgaagat
     4321 ggccgcgggc acgcgccacc ggtatggatc gtcgagatgc gccaagacga ccgtcgcgcc
     4381 cgtcctgata ccctcgccct cgtacagcgt ctcgtgacca acccgcaccc cggcgacgtc
     4441 ggtgatggcg ttgtgcggcc cgggcgccaa taccccaggc acgatcccga gttcccgcac
     4501 gcggcggcgc cccgctaccg acgaagagac ggacataccg cagactactc tcgtgccgcg
     4561 gtgaccacgc acttgcgccc ggccccgcgt gaaccgatga ttgaggtaag cgcgtcctgc
     4621 cacgcccacg gggcacgaca ggtcaacgca cggaaaggca ccagatgagc gatcaacagt
     4681 ccacgcccgc cacccctgcc atcgtcaggg gcacgccgct caagtacctc gacaaggcgc
     4741 tcggcggtct gcgcgacctc ggcctcctcc cggagaacac cacgaaggag gaggcgccca
     4801 tcgtcgccct cctcagccag atcaccgacc tcgaccagga gcgcgtcgtc gccatcactc
     4861 gcaccctcgc ccaggcgtcg ctcttcaacg acgtcgtgcg caagcaggtc gaggcgatgg
     4921 aggtcggctc ccgctacgag gacgtcacca agtcgttcaa cagcatccgc gacgacgcca
     4981 aggccatggt cgaccagctc gaggacggca agatcagcac cttcgagcgc ctctcgaaca
     5041 tctggatgaa ggtcacgcgc ggcgacatct cgagccggtt cgacaagatc aagcacacgt
     5101 acctggaggt cacgaaggac gcccaggacc agatccagcg cgagcacatc atcctcgagg
     5161 cgtaccgcga cttccgcggg gccctcaagg agtccgaggt cctcgcgttc gaggtgctca
     5221 agaagggcga ggcccagctg gagacggcca agcagggcgt gaacgaggcc atggcggcgg
     5281 tcgacgccta cgccggcgaa gacccggccg aacgcgcccg cctggagctc gcgcgcgacg
     5341 agcgcgtgcg cgcgctgcaa gaagtcgaca agcgctacca gatcgccaag gacctctccg
     5401 acaacctgac gatctcgtac aacacgtccg aggtcgtgat ggcgcgcctc atgcagacga
     5461 acacggccaa ggagcgcgtg agggcgcagg ccgtatcgtt cttcagcacg aacgagaccg
     5521 tcctgacggc gctgacggcg acgttcaccg gcctcttcgg cctcaacgag tcgacccgca
     5581 ccctcgaggc catgacgaag ggcgtcagcg actcgctcga cacgctctcc gacatcggcg
     5641 gcaaggtgca ggaggcggcc ctcaaggcgg gctacggccc caccgtgcgc gccgagtccg
     5701 tcaagcgcct ggtcgagtct gtcgtcaact accaggagcg ctcgctcgag atcatcgacg
     5761 agatgcgcaa gcaggccacc accaacgccg ccgagatccg cgatgccgtc gaggacggca
     5821 agcgccggct cgctcggttg gccgagcagg gggccaagct ggacatcatc gacgcgccca
     5881 ggagcgcgac ccagggcggc gctgggcagc tagacagcgc cgagcaagtc ggcggcgccg
     5941 agcgagtcgg cggcgacgtt ggctgacgcg gccgccagcg cgggtccgca accggcgcca
     6001 ccgccgctcg acgacgtcat gctggccatg gacgtcgtcg acaccctgcg ccaccgcgag
     6061 cgcctcgtag aggcagagct ggcttctgag gggcgcgacg aggccatgct cgagagcctg
     6121 cgtaacatct acgcctcgca aggcatcgcc gtgtcggacg aggtcctgcg ccagggcgtg
     6181 gcggcgctcc gcgagggccg cttcgtgtac tccccgccgg cgcgcggccc cggcacgcgc
     6241 tgggcgtacg cctacgtgaa ccggggggcg tggggcaagc tcctgctcgc ccttctcgtc
     6301 gtggccgcgg tgctcgtgtt cggctacgac tcgctcgtgc gcgcgccgca ccggaacctc
     6361 gtgcgggacg tcggtaaggt ctacgcggag gtcgtggccc ggtcgcagga cgccgcggcg
     6421 aaccagcagg ccgacaccct ctacggggtc gctaccggcg cgctcgccaa gggcgacgac
     6481 cgcacggccc gcaccacgct ggcctcgctc aagaccctcc aggagacgct gaacagctcg
     6541 tacgtcctgc gcatcgctac ggacacgacg ggcgtgtggc gcgttcccga cctcaaccag
     6601 gacgccgcca actactacat catcgtcgag ccggtcgacc gcaacggcaa caacgtggcg
     6661 gtcacggtca cgaacgagga gaccggcagg tccgagaccg tgaagcgctt cggcctgcgg
     6721 gtcagcgagg caacgttcga ggccatcaag gccgacaagc tggatgacgg catcatccag
     6781 aacgacgtgt tcggcgagaa gagcgccggc cacctccagc ccgtctatcg tttcgacacg
     6841 acgggcgccg cgataacgag ttgggactga tgatcagcgg ccgcgacact ctcaacacgc
     6901 tcctgtccac gatcgcggag gagcagcagc gcatgcgtca ggtcgacgcg tcgctctcca
     6961 cgaacaacga cgagctgctg aagctcgact cctcgcgcat gcaggagctg cagcgcctcg
     7021 ctcgcatgcg tctcaactac cttgccaacg acaagacgag ggtcgaagac acgtccgacc
     7081 agcagctgct caagttcata gcgcagcggg actccgccta cggcgcgctc cagcaacgcg
     7141 tctcggagct ggaggagaag agcaaggcgc tggaagccga gcgggcgggc gcaatggcca
     7201 acctggacgc ggccgccaag gccatcgagg acgccgaagt ggcgacgcag cgccgcctcg
     7261 agtccgatgc cggctaccaa gccaagctgg aggcgacgcg cgaagccgac cgcgtcgcgg
     7321 gccgcgccga cgccaaagcg actcagagcg agagcgagca ggccaccaag ggcgaggcgt
     7381 accgcgccga cccgctcttc tcctacctct ggcggcgcaa gtacggcacg gccgaatacc
     7441 ggtccggcgg cttcatcttc gcgccgctga tccggtacct cgacgggcgc gtggcccgtt
     7501 tggtcggtta ccaggacgcc agggccaact acaaccgcct gaccgagatc ccgttacgcc
     7561 tgcgcgagca cgccaccaac gcccgcgcgg ccgccgatgc cgcgcacgcc gaactggagc
     7621 gcctagacgt cgaggcgcgc aaggccgatg gcatcccggc gctcgaggac aagaaggccg
     7681 ccgtcgaagc cggcattgcg aagctcgacg ccgccatcca ggaggcgagc gaacgacacg
     7741 ccgccgccat ggcggagagc gagaagttcg ctaagggcgc ggacgagaac tacctgaacg
     7801 ccgtcgagtt catgcgcacc cagttggagg cggctcccct cgatgtcctg cgcgttcggg
     7861 cgttggctac cccgtcaccg gaggacgacc tcgtcgtgag ccggctggcc ggtttcgcca
     7921 agcgccgcga ggagctcgag cgcaccgtcg ccgagctgcg cgccggcgcg gaggcgagcc
     7981 acaagcgcgc ccaggagctc gattggctgc gcaccaacta cacgcgcagc ggcttcgacg
     8041 cgcccaacac ggcgttccgc gacgcgggcg ccgtcaagac cggcctgacg cagtacctca
     8101 cgggcctcat caccatggac gcgctgtggc gcctgctcac ccagcagcgc acggcggtgc
     8161 cgaccagggt cgacccgacc ttcggctcgg gcgggttcgg acgcggaacg gtctggggcg
     8221 ggggcacgac acccccgcca gtgaacccgg ggcctgtcat agatgtcatc ggcagcgtcc
     8281 tcgaaggcat cttcggggcc ggcggcggtg tcggcggctc gtcgcgcggc agttcgtcgc
     8341 gtggtggctc gggtgggttc ggcggtgggt tcggcggctc gtcgcgtggt ggctcgggtg
     8401 ggttcggcgg cggctcgaag ggcggcggct cgcggcccag cggcggaagc a
//
LOCUS       DAOQYE010000006         7351 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_10499_length_7351_cov_3.436541, whole genome shotgun sequence.
ACCESSION   DAOQYE010000006 DAOQYE010000000
VERSION     DAOQYE010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 7351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7351
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10499_length_7351_cov_3.436541"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="PKN52_00400"
     CDS             complement(<1..530)
                     /locus_tag="PKN52_00400"
                     /inference="COORDINATES: protein motif:HMM:NF028259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98422.1"
                     /translation="MSLFRLRTLGALRSLAARPAWLVVGSLMLLGIGYAIFAGVAAGV
                     RWLYAYPLVDRLAPAVLQRSLEGLFLMLMAAVLFSVLVASIGILYGSADLELLLAQPT
                     SRARVFGMKVVELFVNAAGLPLLFTLPVLAGIGAALGACAGYYLVSALAACALYALPV
                     AVGAVLALVLVRVAPAG"
     gene            complement(527..1303)
                     /locus_tag="PKN52_00405"
     CDS             complement(527..1303)
                     /locus_tag="PKN52_00405"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ98423.1"
                     /translation="MLEVVGLTKRYGRNAAVDGLDLVARPGEVLALLGPNGAGKTTAI
                     RCVTGLIKPSGGRILIGGHDVEREPLAAKTLTGFVPDRAWFYPKVTARELLRYLAGVR
                     GLASAEGRMEELLARFRLTRAADSLIETFSHGMRQRLAFCAALIGAPRLFIADEPMVG
                     LDVQGHRDVKLLFRELATDGLAVILTTHTLAVAEEVADRIAVIDRGKLVAHGTLRELR
                     ALLNRSDETSLEELFLILTEAQGEDGERALEPNRRGRQRA"
     gene            complement(1329..1754)
                     /locus_tag="PKN52_00410"
     CDS             complement(1329..1754)
                     /locus_tag="PKN52_00410"
                     /inference="COORDINATES: protein motif:HMM:NF013777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNQ98424.1"
                     /translation="MSAETGAWCPVYSSIELFQEKWVLHIVRALLGGPHGFNELSRAV
                     GGANTTTLSQRLEHLERLGIVDKTVESTMPPRTRYELTHAGRELEGIIAAIDSWARAH
                     MRRCQEHGASGVAFGPEERVPHGEPGAWDDHGAQRERDA"
     gene            1851..2381
                     /locus_tag="PKN52_00415"
     CDS             1851..2381
                     /locus_tag="PKN52_00415"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HNQ98425.1"
                     /translation="MSLVLDTTHSGIEFSVRHLGIATVRGNFTKFDVKATADEAGIPT
                     SIEVDIDAASITTGVENRDNHLRSPDFFDAGNYPTVTFRSTSIAPKGDEYEVKGDLTI
                     RGVTKPVVLHAELTGFVKDPWGNARAAAEGSAKFNRADFGLTWNQVLEAGGLMVSEEV
                     KVSFSVQLVEKAEVAA"
     gene            complement(2578..4821)
                     /locus_tag="PKN52_00420"
     CDS             complement(2578..4821)
                     /locus_tag="PKN52_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98426.1"
                     /translation="MKQLGVLLTVLSTICLSACGGSGTRPQPSEGRTVTGQVVAPVGF
                     EVDGLSVVTPFGVTPVGPDGTFSVNLPSDAQLEFGVETADGELLLLGVDEGDTVTLSL
                     ESTAEALLYYAVGGMWLSANNQQPLRTLLRGRTEVAELAAALEAVATGGGNGLADGDA
                     RVDAALQAAHASLVAGIEPADLEPAANNILINPTGVRFGAELLHADSGVGVVIQNHLR
                     RPAGAMAYETAWTDADGGVHDVSPPRPVALIDVPATSRLELLTAIGDVLTGNAPWTPV
                     LSPEMTLPVRPEAASGTFEVVLLGPSLSGALSPIMSDSRFATRLDQWDEIINAKTMEM
                     FLDQMVLPAVQALTLGHIANVDAAKLAAAKARILRLHDPYALQLGLYLKAGGARGYAD
                     ALRYLISEMVDNRTYRTSMLDIMSGVMNESDRLKANAEAFEKGFQARASASAITAALE
                     TALVGGDVARTMHDLASTISAINWTVEATPALFALAPGRATVTKNSPSIRFTASPRGK
                     VEGNFLFRWSTSGKYGALSDLLSGDHKSSFVTDSREVWYFHDAPARISNDDVDTVTLE
                     VFMVDEGVKTIPPGAEPVARQAATVRGLKTDLDADIVVTYGVTTDYEFYKGAACVEMF
                     LRIPYQPGAGTYTVSFERTQGVGDPMTRNDELRTRPNYSYQFRNDGWPDFKPDGIPTW
                     KGPCVFKNKDGGSAMPTDRFALAYDPEEQMYMYRIFHSLSGDFDAYVRHWYDVLSPPA
                     KVTATYR"
     gene            5057..6814
                     /locus_tag="PKN52_00425"
     CDS             5057..6814
                     /locus_tag="PKN52_00425"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNQ98427.1"
                     /translation="MTFRFLGPDPLEELFRLALVGLQQGKRPRDLEKQSIDFKEERGR
                     RGKSGEVLPGMPRNEQAAAHLAEELACMANTPGAGMVVVGISDEGERIGTNLDPEWLR
                     FRIYELTENKLTVDVREAILDGVRILVIRAPQAVEPIRYKGKLRWRVADHCVEVDASA
                     WASGRLGSVGLDWSGQRSGHVASDVSPRALEVARKFLTQGGPDQAGSNLAEATDIDLL
                     RRLNVLDADGYLTNAGSLLFVATPVPALDYIRREFPGGDSEQRVIESGSLLAQLDAVE
                     SASRLANRIPPHVGTGLVHTQHRALPPRSIREAVVNGVLHRDWQSPAPTTVEHVGDNL
                     TVTSPGGFVGGITAANIITHPSTPRYRHLASVVAHLRLAEREGIGVDRMFADMVRLGH
                     EPPEIKQLPGPFVRVALIGGPASGEWLTFLEQLGREPVRDLDILLLLDMVTRRGWFDW
                     ATAGIVLQKGPAETADAVRRLEVIKVGGTPAVVPVAGVPMNTPPAWRLSDLMVGAISG
                     RVGPLRGKAGRSGLLLDWAFKRGRVSSTEAADLLGVSIGYAGNILGVLEQEGVLQAGR
                     SERRGRGFFYIPANHECGA"
     gene            6877..>7351
                     /locus_tag="PKN52_00430"
     CDS             6877..>7351
                     /locus_tag="PKN52_00430"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNQ98428.1"
                     /translation="MERFAAAASSRGRRLVVGALVTDGAGRIYVQRRSLTRAVFPGCW
                     DLVGGHVEPGEEVVEALARELREETGWSLAEVGPVVEVLDWEAGGVARSEVDFLVTVS
                     GDLARPRLEPDKHSEGRWLAADELGVLLEGRRPDDRWVHDVVARGFGVLRGTWAGG"
BASE COUNT         1190 a         2367 c         2540 g         1254 t
ORIGIN      
        1 ccggctggcg ccacgcgcac cagcacgagc gccagcacgg ccccgaccgc caccggcagg
       61 gcgtaaaggg cgcaggccgc gagggcgcta accaggtaat agccggcgca ggcgcccagt
      121 gcggcaccga tgccggccaa cacgggcagg gtgaagagca ggggtaggcc ggcggcgttg
      181 acgaagagct cgacgacctt catgccgaac acgcgggcgc ggctggtcgg ctgggcgagc
      241 agcagttcca ggtcggcgct cccgtagagg atgccgatgc tggctacgag cacgctgaac
      301 aggacggccg ccatcagcat gaggaagagc ccttcgaggc tccgctgcaa gacggcgggc
      361 gccaggcggt cgacgagcgg gtaggcgtag agccaccgca cgccggctgc tacaccggcg
      421 aagatggcgt acccgatgcc cagcagcatc agggacccca ccacgagcca agcggggcgc
      481 gcggccaggg agcgcagggc ccccagcgtg cgcaggcgga agaggctcac gctcgctggc
      541 gcccgcgccg gttcggctcc agtgcccgct ctccgtcctc cccctgcgcc tccgtcagga
      601 tcaggaagag ctcctcgagc gaggtctcgt ccgaccggtt gagcagcgcc ctcaactccc
      661 tcaaggtgcc gtgcgcgacc agcttgccgc ggtcgatgac ggcgatccgg tcggcgacct
      721 cctcggcgac ggccagcgtg tgcgtggtga ggatgacggc gaggccgtcg gtcgcgagct
      781 cccggaagag gagcttgacg tcgcgatgcc cctggacgtc gagcccgacc atgggctcgt
      841 cggcgatgaa gagcctcggc gcgccgatca gcgccgcgca gaacgccagg cgttggcgca
      901 tgccgtggct gaacgtctcg atgaggctgt cggcggcgcg cgtgaggcgg aagcgcgcca
      961 gcagctcctc catgcggccc tcggcgcttg cgaggccccg gacgcccgcc agatagcgca
     1021 gtagctcgcg cgccgtgacc ttcgggtaga accaggcccg gtcgggcacg aagcccgtga
     1081 gggtcttggc ggcgaggggt tcgcgctcca cgtcgtggcc gccgatgagg atgcggccgc
     1141 cgctcggctt gatgaggccc gtgacgcacc ggatggctgt cgtcttgccc gcgccgttgg
     1201 ggccgaggag cgcgagcacc tcgcccggtc tcgccacgag gtcgaggccg tcaaccgccg
     1261 cgttgcggcc gtaacgtttg gtaagcccga cgacctctag cacggcttcg gggctgcgac
     1321 cgacccggtc aggcgtcgcg ctcccgttgg gcgccgtggt cgtcccaagc cccgggctcg
     1381 ccgtgaggaa ctcgctcctc cgggccgaag gcgaccccgc tcgcgccgtg ctcctggcag
     1441 cgacgcatgt gggcgcgggc ccaggagtcg atggcggcga tgatcccttc gagctcgcga
     1501 ccggcgtggg taagctcgta gcgggtgcga ggtggcatgg tcgactcgac ggtcttgtcg
     1561 acgatgccta ggcgctctag gtgctcgaga cgctgcgaca gcgtggtggt gttggcgccg
     1621 cccaccgcgc ggctcaactc gttgaaaccg tgcggacctc cgaggagggc gcgcacgatg
     1681 tgcaagaccc acttctcctg gaacaactcg atggagctgt agacggggca ccaggcgccg
     1741 gtttctgcgc tcatgagagg tatcctagca cccggagtgc ttgggatgtt gtggcacttg
     1801 acattctaga gtgctacatg ggatgatgct gcctcgataa ggagcctcac atgagcctag
     1861 tcctagacac cacccacagc ggcatcgagt tctctgtacg tcacctcggc atcgccacgg
     1921 tgcgcggcaa cttcacgaag ttcgacgtca aggccaccgc cgacgaggcg ggcatcccca
     1981 cctcgatcga ggtcgatatc gacgccgcga gcatcaccac cggggtcgag aaccgcgaca
     2041 accacttgcg cagccccgac ttcttcgacg ccggcaacta cccgaccgtg actttccgct
     2101 ccacgtccat cgcccccaag ggcgacgagt acgaggtcaa gggcgacctg accatccgcg
     2161 gcgtcactaa gccagtcgtt ctccacgcgg agctcaccgg cttcgtcaaa gatccctggg
     2221 gcaacgcgcg cgcggccgcc gagggcagcg ccaagttcaa ccgtgccgac ttcgggctca
     2281 cctggaacca ggtgctcgag gcgggcggct tgatggtcag cgaggaagtg aaggtcagct
     2341 tctcggtcca gctcgtagag aaggcggagg tcgccgccta ggccggtcgg agacaagcaa
     2401 ccttgcccgg cggcggggtg ttcggtgcgt ctttcgccaa gacgcgaccg cgcaccccgc
     2461 cgtgccagtt ggccgcgcga cggcgccgtg ccctcgcttc gtgcccgggc acagcgcccg
     2521 cgagtcgtgc ccgggcacag cgcccgctta agccaggttc gtagccgtgg cgcgccgcta
     2581 ccggtacgtc gcggtgacct tggcaggggg cgagaggacg tcgtaccagt gccgcacgta
     2641 ggcgtcgaag tcgccgctca atgagtggaa gatgcggtac atgtacatct gttcctcggg
     2701 gtcgtacgct agcgcgaacc gatccgttgg catggccgac ccgccatctt tgttcttgaa
     2761 cacacacggc cccttccacg tgggaatgcc gtccggcttg aaatcgggcc aaccgtcgtt
     2821 gcggaactgg tacgagtagt tggggcgcgt gcggagttcg tcgttgcgcg tcatggggtc
     2881 ccccaccccc tgcgtccttt cgaagctgac tgtgtacgtg ccggcacccg gttggtaggg
     2941 gatcctcagg aacatctcca cgcaggcggc gcccttgtag aactcgtagt cggtcgtcac
     3001 cccgtacgtg acaacgatgt cggcgtcgag gtcggtcttc aggccccgca ccgtcgccgc
     3061 ctgcctcgct accggctcgg cgccgggagg gatggtcttg acgccctcgt ccaccatgaa
     3121 cacctctagc gtgaccgtgt cgacgtcgtc gttggagatg cgtgcagggg cgtcgtggaa
     3181 gtaccagacc tctctcgagt cggtgacgaa gctcgacttg tgatcgcccg acagcaggtc
     3241 ggagagcgcg ccgtacttgc cgctcgtgct ccaacggaag aggaagttcc cctcgacctt
     3301 gccgcgcggg ctggccgtga acctgatgga cggggagttc ttcgtgacgg tggcccgacc
     3361 cggcgcgagc gcgaacagcg cgggcgtcgc ttcgacagtc cagttgatgg cgctgatcgt
     3421 cgaggctagg tcgtgcatgg tcctggcgac atcgccgcct accaacgccg tctcgagggc
     3481 ggccgtgatg gcgctggcgc tagcgcgggc ctgaaagccc ttctcgaagg cctcggcgtt
     3541 ggccttgagg cggtcgctct cattcatgac gcccgacatg atgtcgagca tgctcgtgcg
     3601 gtacgtccgg ttatcgacca tctcgctgat caggtagcgc agggcgtcgg cgtagccgcg
     3661 cgcgccacct gccttgaggt acaggccgag ttggagcgcg tacgggtcgt gcaacctgag
     3721 gatgcgcgcc ttggccgcgg ccaacttggc ggcgtcgacg ttagcgatgt gcccgagcgt
     3781 gagcgcttgc acggcgggga gcaccatctg atcgaggaac atctccatgg tcttcgcgtt
     3841 gatgatctcg tcccactggt cgaggcgcgt tgcgaagcgc gagtccgaca tgatcggcga
     3901 gagtgcgccg ctcaagcttg gcccgagcag cacgacctcg aacgtgccgc tggcagcctc
     3961 cggtcgcact ggcagggtca tctcggggga gagcacgggc gtccacggcg cgttgccggt
     4021 cagcacgtcg ccgatggccg tgaggagttc gagcctcgac gtggcgggga cgtcgatgag
     4081 cgcgaccggt ctcggcggtg agacgtcgtg aaccccgccg tccgcgtccg tccaagccgt
     4141 ctcgtaggcc atggctccgg caggtcgcct gaggtggttc tggatcacca cgcccacgcc
     4201 ggaatcggcg tgcagcagtt cggcaccgaa cctgaccccg gtcggattta tcaggatgtt
     4261 gttcgcggcg ggctccaggt ccgcgggctc gatgccggcg accaaggagg cgtgcgccgc
     4321 ctgcagagcc gcgtcgaccc gtgcgtctcc gtccgccagg ccgttgcctc cgccggtcgc
     4381 gaccgcctcg agcgcggcgg cgagctctgc cacctcggtc cgccctcgta ggagcgtcct
     4441 caacggctgt tggttgttgg cgcttagcca catcccgccc accgcgtagt agaggagggc
     4501 ctcggcggtc gactccaacg acagcgtcac cgtgtcgcct tcatcgacgc cgaggaggag
     4561 gagttcgccg tcggccgtct cgacaccgaa ctccaactgc gcgtcgctcg ggaggttgac
     4621 tgagaaggta ccgtcggggc caaccggcgt cacgccgaac ggggtgacga cgctgaggcc
     4681 gtcgacctca aacccgaccg gcgcgacgac ctgcccggtg acggtgcgac cttccgaagg
     4741 ctgcggcctt gtaccgctac ctccgcaggc acttaggcat atggtcgata agacggtgag
     4801 caacacgcct agttgtttca tgatggcaac ctagtacgct cacgcttaat atccgtttaa
     4861 acggcggtgg cagacgcctc gtcggcggcg ctctcgccgc cgacggcgcc ggccgcttcc
     4921 gagttcgact gcttcgtctg aacggcctac gccttcccgg actcggtgtg ttatttcacc
     4981 ctcggaaaat cacactgatt agccagaagt gcccggatcg tgtggatttt ccgcaggacc
     5041 cttctggagg gcagggatga cgttcagatt cttggggccg gacccgttgg aggagctatt
     5101 ccggctcgcc ttggtgggcc tccagcaagg gaagcgacct cgcgacctgg agaaacagag
     5161 tatcgatttc aaggaagagc ggggccggcg aggcaagtcc ggcgaggtgc tgccgggcat
     5221 gccgcggaac gagcaagccg ctgcgcactt ggcggaggag cttgcatgca tggccaatac
     5281 tcctggcgcc gggatggtag tggtcgggat atccgacgag ggagaacgaa tcggtaccaa
     5341 tcttgatcca gagtggctca ggttccggat ctacgagcta acggagaaca agctcaccgt
     5401 ggatgtacgc gaagccattc ttgacggtgt ccgcatactt gtgatcaggg cgccccaagc
     5461 tgtcgaaccc atcaggtaca agggcaagtt gcgctggcgg gtcgccgatc attgcgtaga
     5521 ggtcgacgcc tcggcctggg cgagcggcag gttgggctcg gtggggctcg attggtccgg
     5581 acagcgttct ggacacgtcg ccagtgacgt aagcccgcga gcacttgaag tggcacgcaa
     5641 gttcctcact cagggcggtc cagaccaggc cggttcgaac ctggcggagg ccacggacat
     5701 cgacctcctg cggcgcttga acgtcctcga cgccgatggt tacctgacca acgccggctc
     5761 gctactgttc gtggcaacac ctgtcccggc cctggattac atccggcggg agtttcctgg
     5821 tggcgacagc gaacagcgag tcatcgagtc aggctctttg ctggcccaac tcgatgcggt
     5881 ggagtcggcg tcccgcttgg ccaaccgtat ccctcctcac gttggcaccg ggctcgtgca
     5941 tactcagcat agggcgcttc cgccacgatc catacgggaa gcagtcgtca acggtgtgct
     6001 tcaccgcgat tggcagtcgc ccgcaccgac gaccgtcgag cacgtcggcg ataacctcac
     6061 ggttacgtcg ccaggcggct ttgtcggtgg aattaccgcg gcgaatatca tcacccaccc
     6121 atcaactccc cgctacaggc acctcgccag tgtagttgcc caccttagac tcgccgaacg
     6181 cgaggggatc ggggtggacc gcatgttcgc cgatatggtt cgactaggcc acgagccgcc
     6241 ggagatcaag cagctgccgg gaccgttcgt tcgcgttgct cttattggcg ggccggctag
     6301 tggagaatgg cttacgttcc ttgagcagtt ggggcgggag cccgttcgtg acctcgacat
     6361 cctgctgctg ctcgatatgg tcacaagaag aggatggttc gactgggcca cggcaggtat
     6421 cgttctccag aagggtccag ctgagaccgc cgatgctgtc aggcggcttg aggtgatcaa
     6481 ggtcggtggg actccagccg tcgttcccgt ggctggcgtg ccgatgaaca ctcctccagc
     6541 ttggcgactc tcggacctga tggtcggtgc gatctccgga cgagtcgggc cgctacgcgg
     6601 caaggcaggt cggagcggcc ttctgctgga ttgggctttc aaacgtggcc gagtttcttc
     6661 gacagaggcg gccgacctcc tgggcgtatc tattggctat gctgggaaca ttcttggcgt
     6721 tttggaacag gaaggcgtac tccaagccgg gcgcagcgaa cgaagaggtc gcgggttctt
     6781 ttacattcca gctaaccacg agtgtggtgc ctgaccatgc ggtcttgcca ggatgatcca
     6841 ctactgaagg tcggtagtcg agccgaggcc ccgacgttgg aacggttcgc cgccgccgcg
     6901 agttctaggg gcaggcgcct cgtcgtcggc gcgctcgtga ccgacggcgc cggccgcatc
     6961 tacgtccagc gccgcagcct cacgcgagcc gtgttcccgg ggtgctggga cctggtcggg
     7021 gggcatgtgg agcccggcga ggaggtcgtc gaggcgctcg cgcgcgaact gcgcgaggag
     7081 accggctgga gcctcgcgga ggtcggcccc gtcgtggagg tgctcgactg ggaggcgggc
     7141 ggcgtggccc gcagcgaggt cgacttcctc gttacggtct ccggcgacct ggcgaggccg
     7201 cgcctggagc ccgacaagca cagcgagggc cgctggctgg cggccgacga gctcggggtg
     7261 ctgctggagg gacgccggcc cgacgaccgg tgggtgcatg atgtcgtcgc tcgcggtttc
     7321 ggggtgttgc gcggcacctg ggcgggcggt c
//
LOCUS       DAOQYE010000007         6974 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_11208_length_6974_cov_3.119381, whole genome shotgun sequence.
ACCESSION   DAOQYE010000007 DAOQYE010000000
VERSION     DAOQYE010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 6974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6974
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11208_length_6974_cov_3.119381"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            61..>791
                     /locus_tag="PKN52_00435"
     CDS             61..>791
                     /locus_tag="PKN52_00435"
                     /inference="COORDINATES: protein motif:HMM:NF013974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HNQ98429.1"
                     /translation="MMAARSTGWGMLFATSVQEAHDLALIAQAATLRSRVPFLHVQDG
                     FRTSHEVARIATLTDDDLRAFLPDELIAAHRARSLDPDRPVLRGTAQNPDVFFQSREA
                     ANSYYLAVPAVVAELFEEFGARTGRAYRAFDYLGAPDAERVLVAMGSGVVAAREAVQK
                     LVAAGEKVGLVAVRLYRPFDSEAFVAALPATVRGVVVLDRTKEPGSAGEPLYQDVITA
                     LAEGGRTLRVVGGRYGLSSKEFTPA"
     assembly_gap    792..801
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <802..3541
                     /locus_tag="PKN52_00440"
     CDS             <802..3541
                     /locus_tag="PKN52_00440"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HNQ98430.1"
                     /translation="PLYQDVVTALAERWPEGARRPAGGRRSAAGARPRVLNGRYGLGS
                     KEFTPRMAKAALDALSDASAPRSFTVGIADDVTRLSLAVDPDFTTERPDVTQAVFYGL
                     GSDGTVGATKNSVKIIGEHTDLDAQGYFVYDSKKSGAVTVSHLRFGPSPVASPYLIEA
                     AGFVGVHWFELMYSRDALSVAAPGATVLFNAPFGPAELWEYLPVEAQRTVRERGLKLY
                     TIDGDRVAREAGLGSRVNTIMQTCFFALSGVLPRDAAIAAIKAAIDRTYGKRGGTVLA
                     RNHAAVDAALANLNEVSVPAQGEGRYRRLALVPDHAPDFVQRVTARLMAGEGDLLPVS
                     AFPVDGTYPTDTARWEKRSIASEVPIWRKDICTQCNLCALACPHSAIRVNAVPAAFLE
                     GAPDGFEWTTWTGKDESLAGHAYVVQLAPDDCTGCGVCVDVCPARDRTDLKRKAINLE
                     PKLEHLERERANWEFFERLPYPRPSFDTLDPIKASQLREPLFEFSGACAGCGETPYLK
                     LLSQLFGDHLIVANATGCSSIYGANLPTAPWAQGPDGRGPAWNNSLFEDAAEFGLGMR
                     LAVDAKSEQALDLVASLRGRIGEELADTLARGVGARDDAGIAAQRGRVAALLTRLDEL
                     EGAEVVGSAVGDPVSVGYMVGDNTGGADGADVRERAGGLTPSELRDVARLRTVAETLV
                     YRQLWIVGGDGWAYDIGFGGLDHVLASGRDVNVLVLDTEVYSNTGGQASKATPRAAVA
                     KFAASGKAVGKKDLGQIAMAYGDVYVAQIALGASPRQTIEVLRAAADWPGPSLVIAYS
                     PCIAHGIEMSTMMTHQRDAVRAGYITLYHYDPREALKEGGGQPLVLDSRPPKGSLAEW
                     AATEGRFSVLARTQPERAAALMELAEADAKARRALYEQLAAVRHEVPGKAGGEAEA"
     gene            3545..4561
                     /locus_tag="PKN52_00445"
     CDS             3545..4561
                     /locus_tag="PKN52_00445"
                     /inference="COORDINATES: protein motif:HMM:NF005741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HNQ98431.1"
                     /translation="MNPDLETHYLGMRLRSPVVASSSPLTGRIDTLRRLDDAGVGAVV
                     LPSLFEEQIEREELLLEDLNAIGREISPEVTGYLDPFTGAPLAVDDYLRHIAAAKDAV
                     GVPVIASLNGHRPGGWTRYARELEWAGADAIELNTYFLATDPAVGAEEIERRTCDIVH
                     AVTSEVGVPVSVKLGPWFSSLPHFVGRLAGAGASGVVLFNRFYQPDIDLDELDVVPSL
                     VLSTSAEARLALRWVAILRGRVHLDLAGAGGVHDGSDIVKLLLAGADVVTMTSALLQH
                     GPEHVNTVLGELQAWMRGRGYDNVSELRGALSQLSAPEPEAFERANYLKALSGYAFGY
                     RVGQ"
     gene            4615..4743
                     /locus_tag="PKN52_00450"
     CDS             4615..4743
                     /locus_tag="PKN52_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98432.1"
                     /translation="MRKRGARTSASAGNRADALRLDLDAGDRKEELKGGTSGKESR"
     gene            4740..>6974
                     /gene="ppdK"
                     /locus_tag="PKN52_00455"
     CDS             4740..>6974
                     /gene="ppdK"
                     /locus_tag="PKN52_00455"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HNQ98433.1"
                     /translation="MSKKWVYLFSEVGIAEDLKGSWDGARALLGGKGANLGDMTRLGV
                     PVPPGFTVSTEACNAYLAAGGRFPDDVWEQELVALRRVEEETEKTFGDPAKPLLVSCR
                     SGAKFSMPGMMDTILNIGLNDDVARGLIALTADPRFVYDSYRRLVQMFGSVVLGVPDE
                     VFEAVITARRNAAGVKVDAELQAVDWQEITRKFKEIVRTYTGAPFPEEPLEQLRLATE
                     AVFRSWNGKRAIDYRNAAGIRHDLGTAVNIQAMVFGNMGEDCATGVAMSRNATTGENR
                     LEGDFLVNAQGEDVVAGIRTTQPLEELADVMPNAFEEFAAIAGRLERHYRNMQDMEFT
                     VEHGKLWILQTRDGKRTAQAEVRIAVDMVEEGLIERSEAVRRVKPEQIDYFLHPQFEA
                     TARKAANKLAAGLNVSPGAAVGIVAFDADTAERWAKDGRDVVMVRPETKPDDVHGMLA
                     AKGILTSSGGRTSHAALVARQFGKPAVVGVAALAIDLDKREMTVGDIVIKEGDVVSID
                     GTVGDVYLGALPTVIPDIDNPYLTKLLAWADAIRTLGVRANADYPADARRARAYGAEG
                     LGLVRTEHMFFEAERLGIVQKMIMARTKAERAEALEALLPLQRGDFEGLFRAMDGQPV
                     IIRLLDPPLHEFLPSHDELTSALADLKMQLQHFRTFEEMDRALSVIRHKQDHLARVTE
                     LREANPMLGTRGVRLGILIPELTRMQVRAIFEAACAVKREGLDVHPEVMIPLVGIEAE
                     IEDNR"
BASE COUNT         1054 a         2555 c         2394 g          961 t
ORIGIN      
        1 cacatcgcgg cacgctccat cgcggcgcac gcgctgtcga tcttcggcga ccattccgat
       61 gtgatggccg cccgttcgac gggctggggc atgctcttcg ccacctccgt ccaggaggcg
      121 cacgacctcg cgctcatcgc ccaagccgcc acactgcgct cgcgggtgcc gttcctccac
      181 gtccaggacg gcttccgcac gagccatgag gtggcgcgta tcgccaccct gacggacgac
      241 gacctcaggg cgttcctgcc ggacgagctc atcgccgcgc accgcgcccg ctcgctcgac
      301 cccgaccggc ccgtcctgcg cggcaccgcc cagaacccgg acgtcttctt ccagtcacgc
      361 gaggcggcca actcctacta cctggccgtg ccggccgtgg tggccgaact gttcgaggag
      421 ttcggcgctc gaaccggccg cgcgtaccgc gccttcgact acctgggcgc gcccgacgcc
      481 gagcgcgtcc tcgtcgccat gggctcgggc gtggtcgccg cgcgcgaggc cgtccagaag
      541 ctcgtggcgg cgggggagaa ggtcggcctc gtcgccgtcc gcctctaccg tccgttcgac
      601 tccgaggcct tcgtggccgc gttgccggcg accgtccgcg gcgtcgtcgt cctcgaccgc
      661 acgaaggagc cgggctccgc cggcgagccg ctctaccagg acgtcatcac cgcgctggcc
      721 gagggcgggc ggacgctccg ggtcgtcggc gggcggtacg gcctgtccag caaggagttc
      781 acccccgcca tnnnnnnnnn ngccgctcta ccaggacgtc gtcacggcgc tggccgagcg
      841 gtggccggag ggcgcccggc gtcccgccgg cggccggcgt tccgccgccg gcgcgcggcc
      901 cagggtcctc aacggtcgct acggccttgg gagcaaggag ttcacgccgc gcatggccaa
      961 ggcggcgctg gacgccctct cggacgcgag cgcgccgcgc tccttcacgg tcgggatcgc
     1021 ggacgacgtc acgcgcctct ccctggccgt cgaccctgac ttcaccaccg aacggccgga
     1081 cgtcacgcag gccgtgttct acggcctcgg cagcgacggc acggtgggcg ccaccaagaa
     1141 cagcgtcaag atcatcggcg agcacactga cctggacgcc cagggctact tcgtctacga
     1201 ctcgaagaag tcgggcgccg tcacggtctc gcacctgcgc ttcgggccgt cgcccgtcgc
     1261 cagcccttac ctcatcgagg cggccgggtt cgtcggcgtc cactggttcg agctcatgta
     1321 ctcccgcgac gcgctctcgg tagccgcgcc cggcgcgacc gtcctcttca acgcgccctt
     1381 cgggcccgcc gaactctggg agtacctgcc cgtcgaggcg cagcggacgg tgcgggagcg
     1441 cgggctgaag ctctacacga tcgacggcga cagggtggcg cgcgaagcgg gcctcggctc
     1501 gcgcgtcaac accatcatgc agacgtgctt cttcgcgctc tcgggcgtgc tgccgcgcga
     1561 cgcggccatc gccgccatca aggcggccat cgaccgcacg tacggcaagc gcggcggtac
     1621 tgtcctggcg cgcaaccacg cggccgtcga cgcggcgctg gccaacctca acgaagtgag
     1681 cgtgccggcg cagggcgagg gccgctaccg gcgcctcgcg ctcgtgcccg accacgcgcc
     1741 ggacttcgtg cagcgcgtga ccgccaggct catggcgggc gagggcgacc tcctgcccgt
     1801 ctcggcgttc ccggtggacg gcacctaccc gaccgacacg gccaggtggg agaagcgctc
     1861 catcgcgtcc gaggtgccca tctggcggaa ggacatctgc acgcagtgca acctgtgcgc
     1921 gcttgcctgc ccgcactcgg cgatacgcgt caacgccgtc ccggccgcgt tcctggaggg
     1981 cgcccccgac ggtttcgaat ggaccacctg gacgggcaag gacgagagcc tggccggcca
     2041 cgcctacgtc gtgcagctcg cgcctgacga ctgcacgggc tgcggcgtgt gcgtagacgt
     2101 ctgcccggcg cgcgaccgga ccgatctgaa gcgcaaggcc atcaacctcg agcccaagct
     2161 ggagcacctg gagcgcgagc gcgcgaactg ggagttcttc gaacggctcc cgtacccgcg
     2221 cccgagcttc gatacgctcg accccatcaa ggcctcgcag cttcgcgagc ccctcttcga
     2281 attctcgggc gcctgcgcgg gctgcggcga gacgccctac ctcaagctcc tcagccagct
     2341 cttcggcgac cacctcatcg tcgccaacgc caccggctgc tcctccatct acggcgccaa
     2401 cctgcccacc gccccgtggg cgcagggtcc cgacgggcgt ggccccgcct ggaacaactc
     2461 cctcttcgag gacgccgccg agttcggcct cggcatgcgc ctcgcggtcg acgccaaatc
     2521 cgagcaggcg ctcgacctcg tcgccagtct gcggggcagg atcggtgagg aactcgccga
     2581 cacccttgcc cgcggggtcg gcgcgcgcga cgacgccggc atcgccgccc agcgcgggcg
     2641 cgtggcggcg ctactgacgc ggctggacga gctcgagggc gcggaggtgg tggggagcgc
     2701 ggtcggcgac cccgtgtcgg tcggctacat ggtcggtgac aacacaggcg gtgccgacgg
     2761 tgccgacgta cgcgagcgcg ccggcggcct caccccgagc gagctccgcg acgtcgccag
     2821 gctccgcacg gtggccgaga cgctcgtcta ccgccagctc tggatcgtcg gcggcgacgg
     2881 ctgggcgtac gacatcggtt tcggcgggct cgaccacgtg ctggcgagcg gacgcgacgt
     2941 gaacgtgctc gtcctcgaca cggaggtgta cagcaacacg ggcggtcagg cctccaaggc
     3001 gacgccgcgc gcggccgtcg ccaagttcgc ggccagcggc aaggccgtcg gcaagaagga
     3061 cctaggccag atcgcgatgg cgtacggcga tgtctacgtc gcgcagatcg cgctcggcgc
     3121 cagtccgcgc cagaccatcg aggtcctgcg cgccgcggcc gactggccgg ggcccagcct
     3181 cgtcatcgcc tacagcccgt gcatcgcgca cggcatcgag atgagcacga tgatgacgca
     3241 ccaacgcgac gccgtacggg ccggctacat caccctgtac cactacgacc cgcgcgaggc
     3301 gctcaaggaa ggcggcggcc agccgctcgt gctcgactcg cggccgccca agggctcgct
     3361 cgccgaatgg gcggccaccg aggggcggtt ctccgtcctg gcgcgcacgc agcccgagcg
     3421 cgccgcggcc ctgatggagc tcgcggaggc cgacgccaag gccaggcggg cgctgtacga
     3481 gcaactcgcg gccgtgcgcc acgaagttcc cggcaaggcc ggcggggaag cggaggcctg
     3541 aaccatgaac ccggacctgg agacccatta cctaggcatg cggttgcgca gtcccgtcgt
     3601 ggcgtcgtct tcgcctctca ccgggcgaat cgacaccttg cggcgtctcg acgacgcggg
     3661 cgttggcgcc gtggtgctcc cgtcgctgtt cgaggagcag atcgagcgcg aggagctgct
     3721 cctcgaagac ctgaacgcga tcgggcggga gatctcgccg gaagtgaccg gctaccttga
     3781 cccgttcacc ggcgcgccgc tcgccgtcga cgactacctc cgccacatcg ccgccgccaa
     3841 ggacgccgtc ggggtccccg tcatcgcgag cctgaacggc caccgcccgg gcgggtggac
     3901 gcgctacgcg cgggagctgg agtgggcggg ggccgacgcc atcgagctca acacgtactt
     3961 cctcgccacc gatcccgcgg tcggcgccga ggagatagag cggcgcacgt gcgacatcgt
     4021 gcacgccgtc acgagcgagg tcggcgtgcc agtgtccgtc aagctcgggc cgtggttctc
     4081 ctctcttcct cacttcgtcg gccgcctagc cggggccggc gcgagcgggg tcgtgctctt
     4141 caaccgcttc taccagcccg acatcgacct ggacgagctg gacgtcgtgc cgagcctcgt
     4201 cctctcgacc agcgcggagg cgcgcctcgc cctgcgctgg gtcgccatcc tgcgcggccg
     4261 cgtgcacctc gacctggccg gcgcgggtgg cgtgcatgac ggttccgaca tcgtgaagct
     4321 gctcctggcc ggggccgatg tcgtgacgat gacgtcggcg ctgctccagc acggcccgga
     4381 gcacgtgaac accgtcctgg gcgagttgca ggcgtggatg cgcgggcgcg ggtacgacaa
     4441 cgtctccgaa ctgcgcggcg cgctctccca gctctccgcg ccggagccgg aggcgttcga
     4501 gcgcgcgaac tacctcaagg cgctctccgg ctacgccttc gggtaccggg tgggccaatg
     4561 agggccggcg gggccggagg tcgtggccgc cagggcgctg gtaaggagcg gtcgatgcgg
     4621 aagcgaggcg cgaggacttc cgcctctgcc gggaaccgtg ccgacgccct taggctcgac
     4681 ttggatgcgg gcgaccgcaa ggaagagctg aagggcggca cgtccgggaa ggagtcgcga
     4741 tgagcaagaa gtgggtctac ttgttctcgg aggtcgggat cgccgaggac ctgaagggca
     4801 gctgggacgg cgctcgcgcg ctcctgggcg gtaagggcgc aaacctgggc gacatgacgc
     4861 ggctgggcgt gcccgtgccg ccgggcttca ccgtctccac cgaggcgtgc aacgcctacc
     4921 tcgccgcggg cgggcggttc cccgacgacg tgtgggaaca ggagctggtg gcgctgcggc
     4981 gcgtcgagga ggagacggag aagacgttcg gcgaccccgc caagccgctc ctcgtctcct
     5041 gccgatcggg cgccaagttc tccatgccgg ggatgatgga caccatcctc aacatcggcc
     5101 tgaacgacga cgtggcgcgc gggctgatcg ccctcaccgc cgacccgcgc ttcgtctacg
     5161 actcctaccg gcgcctcgtg cagatgttcg gcagcgtcgt gctaggggtg cctgacgagg
     5221 tcttcgaggc cgtcatcacg gcgcggcgca acgcggccgg cgtcaaggtc gacgccgagc
     5281 tgcaggccgt cgactggcag gagatcacgc gcaagttcaa ggagatcgtg cgcacgtaca
     5341 cgggcgcgcc gttccccgag gagcccctcg agcagctgcg cctggccacg gaggccgtgt
     5401 tccgctcctg gaacggcaaa cgggccatcg actaccgcaa cgcggccggc atcaggcacg
     5461 acctcggcac ggccgtgaat atccaggcca tggtcttcgg caacatgggc gaggactgcg
     5521 cgacgggcgt ggccatgagc cgcaacgcca ccaccggcga gaaccgcctc gagggcgact
     5581 tcctcgtgaa cgcgcagggc gaggacgtgg tggccggcat ccgcacgacg cagcccctcg
     5641 aggaactggc ggacgtcatg ccgaacgcgt tcgaggagtt cgccgccatc gccgggcgcc
     5701 tcgagcgcca ctaccgcaac atgcaggaca tggagttcac cgtcgagcac ggcaagctct
     5761 ggatcctgca gacccgcgac ggcaagcgca cggcgcaggc cgaggtccgc atcgccgtcg
     5821 acatggtgga ggaaggcctc atcgagcgca gcgaggccgt caggcgggtc aagcccgagc
     5881 agatcgacta cttcctgcac ccgcagttcg aggccacggc gcgcaaggcc gccaacaagc
     5941 tggccgcggg cctcaacgtc tcgcccggcg ccgcggtggg catcgtcgct ttcgacgccg
     6001 acaccgcgga acggtgggcg aaggacggcc gcgacgtcgt catggtccgc cccgagacga
     6061 aacctgacga cgtgcacggc atgctcgccg ccaagggcat cctgacgagc tccggcggcc
     6121 gcaccagcca cgccgccctc gtcgccaggc agttcggcaa acccgccgtc gtgggcgttg
     6181 ccgccctcgc cattgacctc gacaagcgcg agatgaccgt cggcgacatc gtcatcaagg
     6241 agggcgacgt tgtctccata gacggcactg tcggtgacgt gtacctaggc gccctgccca
     6301 ccgtcatccc cgacatcgac aacccgtacc tcacgaagct cctggcatgg gcggacgcca
     6361 tccgcacgct gggcgtgcgc gccaacgccg actaccctgc cgacgcgcgg cgggccaggg
     6421 cctacggcgc ggagggcctc ggcctcgtgc gcaccgagca catgttcttc gaggccgagc
     6481 gcctcgggat cgtgcagaag atgatcatgg cgcgcaccaa ggccgagcgc gcggaggccc
     6541 tcgaggcgct gctgcccctg cagcgcggcg acttcgaagg cctgttccgc gccatggacg
     6601 gccagcccgt catcatccgc ctcctcgacc cgccgctgca cgagttcctc ccgtcgcacg
     6661 acgagctcac gagcgccctg gccgacctca agatgcagct ccagcacttc cgcactttcg
     6721 aggagatgga ccgcgccctg tccgtcatcc ggcacaagca ggaccacctg gcgcgcgtaa
     6781 ccgaactacg cgaggccaac ccaatgctcg gcacgcgcgg cgtgcgcctc ggcatcctca
     6841 tccccgagct gacccgcatg caggtccgcg ccatcttcga ggcggcctgc gcggtcaagc
     6901 gcgaggggct cgacgtgcac cccgaggtca tgatccccct cgtcggtatc gaagcggaga
     6961 tcgaggataa ccgc
//
LOCUS       DAOQYE010000008         6735 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_11712_length_6735_cov_2.777695, whole genome shotgun sequence.
ACCESSION   DAOQYE010000008 DAOQYE010000000
VERSION     DAOQYE010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 6735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6735
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11712_length_6735_cov_2.777695"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..962)
                     /locus_tag="PKN52_00460"
     CDS             complement(<1..962)
                     /locus_tag="PKN52_00460"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HNQ98434.1"
                     /translation="MKTTVPASCRVEALLAAAALVLLAACGGQGPTPAKTPVAPGNVT
                     ATPGPGYITVAWTDNSDDETGFEVYRNATPGPTAMQTGTKVATVPANGTSYIDMDIEL
                     EREYRYTVVAVNGQGSSEEAVAPDGTAVPWHVDLMMGTSRRRSTDEWTASIAIVYLVF
                     PPEVIGSQAVTAYITGPAGWHDDLPWEHVYECPPEQASCYDGSFMFRTSSSIAALTGD
                     YEVSVTIDGTEYTATTHLDASARMDRPTSISVSSVSSSGLDVDWTNPADTQSAIVSIH
                     RGTYGGRVTPYHLMNGDHFSFESLDLEDGAYGVAAAASRYFGKDL"
     gene            complement(1056..1907)
                     /locus_tag="PKN52_00465"
     CDS             complement(1056..1907)
                     /locus_tag="PKN52_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HNQ98435.1"
                     /translation="MPLAIITDSTCDLTAQELASLNVEAVPLHVHFQGGTFKDWLEID
                     PAKIIAGVAQGAGMPSTSQPSPAEFTAAFERALAAGADEVLVITISSGVSGTFQSATI
                     AQKTYGAKVTVFDSLAASLGHGEMVRVAARMRDAGKGVPEIIKALEKIRDTNFVIFTV
                     ATMDYLQKNGRIGRATAFLGGLLNVKPLLTLDGGKVGPLTRARGIKKAQQEMVDRFKA
                     YVAASSGPVVLNLIHVQDPPAAEGLRAAIDAAGVPYEFGGYHEIGAVIASHVGPNTFG
                     LYAHAAA"
     gene            2055..2828
                     /locus_tag="PKN52_00470"
     CDS             2055..2828
                     /locus_tag="PKN52_00470"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HNQ98436.1"
                     /translation="MLEAVWWLLTPSTLLVLLVLLAFAAGRLRAGRLAGALLFFPLLL
                     LLALVLAPLDEYLASGLESRVSAPDPLPERVDGIIVLGGAVEWRVSAARGQLAFDDAG
                     ERLLAALALAKRYPEAELVFTGLFMDALREEWKDASGDGLLFQDAMRGRRVTFLGEAR
                     STYEEALLARERVAPAPGATWLLVTSALHMPRAYGTFKAQGWQVTPFPVDYRSLPSSE
                     WRAAWRFDLAVGKRLAGLDRVVREWGAYQVYKQSGRLVD"
     gene            complement(2910..3935)
                     /locus_tag="PKN52_00475"
     CDS             complement(2910..3935)
                     /locus_tag="PKN52_00475"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HNQ98437.1"
                     /translation="MRVAIVGASGAVGQELLAILAERAFPTTELRLYASPRSAGRQVE
                     FAGAKHTIMALPEDGDLGADIVFSSAGGSVSKAHAWAWAAHGAVVIDNTSAWRMDERV
                     PLVVPEVNAHALADHQGVIANPNCSTIIALMALAPLHRAFGLKRATVATYQAVSGAGA
                     TGITELREQTAAVLAGTSAASGEPATEPPAPRKFRHPIAFNLFSHDSDVGDDGYNGEE
                     RKLLLESRKILDAPNLAVSATCVRVPVFRAHSEAIHAEFERPVSEAEARAVLERAPGV
                     RLVDDRGNNTFPMPLAASGKDDTLVGRLRADSSLPGGLALFVAGDQIRKGAALNAVQI
                     AEALLPD"
     gene            3982..>4180
                     /locus_tag="PKN52_00480"
     CDS             3982..>4180
                     /locus_tag="PKN52_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98438.1"
                     /translation="MSRERGRWRRIVIKVGTSSIVDASGRIAQHKMWDIARGAQVLAA
                     AQDVGGRGSPPARGASGSLGSA"
     assembly_gap    4181..4280
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4301..5362
                     /gene="proB"
                     /locus_tag="PKN52_00485"
     CDS             4301..5362
                     /gene="proB"
                     /locus_tag="PKN52_00485"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HNQ98439.1"
                     /translation="MGPLGSAVGPLGSAVGPLGAAGSGRTTGDRSTGAQFVIVTSGAG
                     AAGRERLGLKLPLTLPQKQAAAAVGQALLMLDWERALAPLPVAQLLLSAGDVQERERY
                     VNAKSALEASLSLGAVPVINENDSVATAELKLGDNDTLSAWVSYLVDADVLVILTDVD
                     GLYDSDPHRDPDARKIEVVSDLAEIAHLGGGAGSTHGTGGMATKLRAARIATGAGIET
                     IVLGGGGAGLEALARGEARGTRFLAAAHVPARKAWIANQPVRGAIHLDDGAVRALRGG
                     KSLLPGGITGVTGLFTFGDAVELRHAGAPIGVGLTNYGSDAVDRIRGRHTRDVAELLG
                     HKDYDEVVHRDNLVLGGAR"
     gene            5385..5792
                     /locus_tag="PKN52_00490"
     CDS             5385..5792
                     /locus_tag="PKN52_00490"
                     /inference="COORDINATES: protein motif:HMM:NF025607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4345 family protein"
                     /protein_id="HNQ98440.1"
                     /translation="MNPRTQLIYVSAAITFGLGLLGLVNPIFTATMLGLDIVDPRGLS
                     QVRATFGMLHLALGALIVRGSLPRPGAGVYLRVSALLVGAVAVGRLLSIALDGAVTLL
                     NLLFLASETVALLGIVLAWLDPGRRRTSPVSEA"
     gene            5789..>6735
                     /locus_tag="PKN52_00495"
     CDS             5789..>6735
                     /locus_tag="PKN52_00495"
                     /inference="COORDINATES: protein motif:HMM:NF024785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HNQ98441.1"
                     /translation="MSKDLKVSGLVDGTRFSPPDVALRREAGLAKLRELYASWGYAPV
                     EVPVLERFDPYHPREEQAFKLIDNGSEVIALRSDFTPALARLVAITHPGVAAGERRPL
                     RFRYAGTVWHANHPEVAGMREFTQVGIELVGVSNARADAEIIHLARESVRALGLAPRI
                     EVGNPAYVKALLEAAQVPREHQGALADAIDRKDRSDVLAAIEALGLRGKAADAILQAP
                     DLYGAESVLIEARRLAPDAAANLALDRLEGVLAEFEDASELLLDLGLARRLSYYTGVT
                     FRAFTPDYGRPLLGGGRYDAALLPYAAGFSMGLERLLGAA"
BASE COUNT          899 a         2305 c         2363 g         1068 t
ORIGIN      
        1 aggtccttgc cgaagtagcg gctcgcggcc gcggcgacgc cgtaggcgcc gtcctcgagg
       61 tcgaggctct cgaagctgaa atggtcgccg ttcatcaggt ggtagggggt cacgcggccc
      121 ccgtaagtgc cgcggtgtat cgacactatg gcgctctgcg tgtcggcagg gttggtccag
      181 tcgacgtcca ggccgctcga cgacacgctc gaaaccgaga tgctggttgg cctatccatt
      241 ctggcagagg cgtccaggtg ggtcgtcgcc gtgtattcag tgccgtcgat ggtcaccgag
      301 acttcgtaat cgcccgttag cgccgcgatg gatgatgagg tgcggaacat gaagctgccg
      361 tcgtagcagc tggcctgctc gggcgggcac tcgtagacat gttcccatgg cagatcgtcg
      421 tgccagccag ccggccccgt gatgtacgcg gtcaccgcct gtgagccgat cacctcaggg
      481 ggaaacacga ggtagacgat ggcgatgctt gccgtccact cgtcggtact ccggcgcctg
      541 ctcgtgccca tcatcagatc gacgtgccag ggtacggccg ttccatccgg tgccaccgcc
      601 tcctccgagg agccttgccc gttcacggcg acgaccgtgt accggtactc ccgctcgagc
      661 tcgatgtcca tgtcgatgta actggtgccg ttcgcgggga cggtcgcgac cttcgttccg
      721 gtctgcatgg ccgtcggtcc cggggtcgcg ttgcggtaga cctcgaaccc cgtctcgtcg
      781 tcgctgttgt cggtccaggc caccgttatg taacccggtc ctggcgtagc cgtcacgttg
      841 cccggagcca cgggcgtctt ggcgggtgtt ggcccctggc cgccgcaggc agccagcagg
      901 acgagcgctg cggccgcaag gagcgcctcc accctgcatg aagcgggaac ggttgtcttc
      961 aaggttcgcc tccctgatcc tcttggtttc agagaggttg catccgtgac cccgtccgcg
     1021 gcccgtccaa ggcgagacgg cgggtctagg cggtcctacg ccgccgcgtg cgcgtacaac
     1081 ccgaacgtgt tcggaccaac gtggctggcg atgacggcgc cgatctcgtg atagccgccg
     1141 aactcgtagg gcacgccggc cgcgtcgatg gcggcgcgca gcccctcggc ggcgggcggg
     1201 tcctgcacgt ggatgaggtt gagcacaact ggccccgagg acgcagcgac gtaggctttg
     1261 aagcggtcga ccatctcctg ctgcgccttc ttgatgccgc gggcgcgggt cagcggaccg
     1321 accttcccgc cgtccagggt gagcagcggc ttgacgttga gcaggccgcc caggaacgcg
     1381 gtggcgcggc cgatgcgccc gttcttctgt aggtagtcca tggtcgcgac cgtgaagatc
     1441 acgaagttcg tgtcgcggat cttctcaagc gccttgatga tctccgggac gcccttgccc
     1501 gcgtcgcgca tgcgggccgc cacgcgcacc atctcgccgt gcccgaggga ggccgccagg
     1561 ctgtcgaaca cggtcacctt ggcgccgtag gtcttctggg cgatggtggc cgactggaag
     1621 gtcccggaga cgccggagga gatcgtgatg acgaggacct cgtcggcgcc ggccgccaag
     1681 gcacgctcga aggcggcggt gaactcggcc gggctcggct ggctcgtgga tggcatgcct
     1741 gcgccctgcg ccacgccggc gatgatcttg gcggggtcga tctctagcca atccttgaag
     1801 gtgccgccct ggaagtgcac gtgcagaggc acggcctcca cgttgagcga ggcgagttcc
     1861 tgggcggtga ggtcgcaggt cgagtccgtg atgatcgcga ggggcattgg ggctccgatg
     1921 ctattgcgag tgggttgtgc tcaacgttcg cggttgtccc aggataaacg accgcggcgc
     1981 cccacgcatg ccgccgcgct cggcacgagt cctccctcat gagccgcgtg cgctctctgg
     2041 ggcatgatgg gaccatgctg gaagccgtgt ggtggttgct caccccgagc accttgttgg
     2101 tgctcctggt gctgctcgcc ttcgccgcgg ggcgcctccg cgcgggtcgc ctggccggcg
     2161 ccctgctctt cttcccactg ctcctgctgc tggcgctggt gctcgcgccc ctcgacgagt
     2221 acctggccag cggcctcgaa agtcgcgtga gcgcgcccga cccgctgccg gaacgggttg
     2281 acggcatcat cgtcttgggt ggcgcagtcg agtggcgtgt cagcgcggcg cgcggccagt
     2341 tggccttcga cgacgccggc gagcgactgc tcgcggcgct ggcgctcgcg aagcgttacc
     2401 cggaagcgga gctcgtgttc acgggcttgt tcatggacgc gttgcgcgag gagtggaagg
     2461 acgccagtgg cgacggcctt ctgttccagg acgcgatgcg tggtcgccgc gtgaccttcc
     2521 tgggagaggc gcgcagcacg tacgaggaag cgttgctggc gcgcgagcgc gtggcgccgg
     2581 cccccggcgc cacctggctc ctggtcacga gcgcgctgca catgccccgc gcttacggca
     2641 cgttcaaggc gcaggggtgg caggtgacgc cgttccccgt cgattaccgc tccttgccgt
     2701 cgagcgaatg gcgcgcggcc tggcgcttcg acctggccgt cggcaagcgc ctggccggac
     2761 tggacagggt cgtgcgggag tggggagcct accaggtcta caagcagtcc ggcaggctcg
     2821 tcgactgatt ctgattcgaa gaccccgagc atcggtggac cggggcggct cgccgccggg
     2881 ccgttggcgc gagcgacgct cagccgacgt cagtccggca gcaacgcctc cgctatctgc
     2941 acggcgttga gggcggcccc cttgcggatc tggtcgccgg cgacgaacag ggctaggccg
     3001 ccggggaggc tcgaatcggc gcggagccta ccgacgagcg tgtcgtcctt gccgctcgcc
     3061 gccaacggca tggggaacgt gttgttgccg cggtcgtcga cgaggcgcac gcccggcgcg
     3121 cgctcgagca ccgccctcgc ctcggcttcg ctcactggcc gctcgaactc ggcgtggatg
     3181 gcctcgctgt gcgcgcggaa caccggcacg cgtacgcacg tcgcgctgac ggccaggttc
     3241 ggcgcgtcca agatcttgcg cgattcgagg aggagcttgc gctcctcgcc gttgtagccg
     3301 tcgtcgccga cgtcggagtc gtggctgaag aggttgaacg ctatggggtg gcggaactta
     3361 cggggcgcag gcggctcggt cgccggttcg ccgctggcgg cgctggtccc tgccagcacg
     3421 gcggctgtct gctcgcgcag ctcggttatg ccggtggcac ccgcccccga caccgcctgg
     3481 taggtggcga cggtggcgcg cttgaggccg aacgcccggt ggagcggcgc gagcgccatg
     3541 agggcgatga tggtcgagca gttcgggttg gcgatcacgc cctgatggtc ggccaaggcg
     3601 tgcgcgttga cctccgggac gacgagcggg acacgctcgt ccatgcgcca ggcgctcgtg
     3661 ttgtcgatca ccacggcgcc gtgagccgcc caggcccaag cgtgcgcctt ggacacgctg
     3721 ccgccggccg aggagaacac gatgtcggca cccaagtcgc cgtcttctgg caacgccatg
     3781 atggtgtgct tcgcgccagc gaactccacc tgcctcccgg cggacctcgg ggaggcgtag
     3841 agcctcagct ccgtcgtcgg gaaggctcgc tccgcgagga tggcgaggag ttcctgtccg
     3901 acggctccgc tggctccgac aatggcgaca cgcatgcggc ggagtgtagc accaagcccg
     3961 ccaggcgata gcatggcccg catgtcgcgc gaacgcggac gctggcgccg catcgtcatc
     4021 aaggtcggga cgagttccat cgtggacgcc tccgggcgca tcgcccagca caagatgtgg
     4081 gacatcgcgc gtggggcgca ggtcctcgct gccgcccagg acgtcggggg gcggggtagc
     4141 ccgcccgcac gcggtgcctc cggctccctc ggcagcgccg nnnnnnnnnn nnnnnnnnnn
     4201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn tgggccccct cggcagcgcc gtgggccccc tcggcagcgc
     4321 cgtgggcccc ctcggcagcg ccgtgggccc cctcggtgcc gccggttcgg gccggaccac
     4381 cggcgaccgg tcgacgggcg cgcagttcgt catcgtcacg tccggggccg gtgccgccgg
     4441 ccgcgagcgg ctcggcctca agctccccct cacgctcccg cagaagcagg cggccgcggc
     4501 cgtcgggcag gccctcctga tgctcgactg ggagcgcgcc ctcgccccgc tgccggtcgc
     4561 gcagctcctc cttagcgccg gcgacgtgca ggagcgggag aggtacgtga acgccaagag
     4621 cgcgctcgag gcgtcgctga gcctcggcgc cgtccccgtg atcaacgaga acgacagcgt
     4681 cgcgaccgcg gagctgaagc tcggcgacaa cgacacgctc tcggcctggg tgagctacct
     4741 ggttgacgcc gacgtgctcg tcatcctcac cgacgtcgat gggctctacg attccgaccc
     4801 gcaccgcgac cctgacgccc gcaagatcga ggtggtgagc gacctggccg agatcgccca
     4861 tctcggcggc ggagccggct cgacccacgg cacgggcggc atggcgacca agctgagggc
     4921 agcgcgcatc gccaccggtg ccgggatcga gacgatcgtc ctcggcggcg gtggcgctgg
     4981 gctggaggcg ctggcgcgcg gggaggcgcg cggcacccgc ttcttggccg cggcgcacgt
     5041 gcctgcgcgc aaggcgtgga tagccaacca gcccgtgcgc ggcgcgatcc acctcgacga
     5101 cggggcggtc agggccctca ggggcggcaa gagcctcctg cctggcggca tcaccggggt
     5161 gacgggcctg ttcacgttcg gcgacgccgt ggagctgcgc catgccggcg cgccgatcgg
     5221 cgttggcctc accaactacg gctccgacgc ggtcgaccgc atccgcggtc ggcacacgcg
     5281 cgacgtcgcc gagctgctgg gccacaagga ctacgacgag gtcgtccacc gtgacaacct
     5341 cgtcctcggc ggggcgcgtt agcgccggtg atagcattcg cgccatgaac ccgcggaccc
     5401 agctcatcta cgtctcggcg gccataacct tcggcctcgg gctcctcggg ctggtcaacc
     5461 cgatcttcac cgcgaccatg ctgggcctcg acatcgtcga cccgcgcggg ctcagccagg
     5521 tgcgcgccac cttcgggatg ttgcatctgg cgctcggcgc cctcatcgtc cggggctcgc
     5581 tcccgcggcc cggggccggc gtctacctga gggtgagcgc cctgctcgtc ggcgcggtcg
     5641 cggtcggacg cctgctgtcg atcgcgctgg atggcgccgt caccctcctg aacctgctgt
     5701 tcctagcgag cgagacggtg gcgctcctcg gcatcgtgct cgcctggctc gacccggggc
     5761 gacgcaggac gtcccccgtc tccgaagcgt gagcaaggac ctaaaggtga gcggcctcgt
     5821 cgacggcacc cgcttctccc cgccggacgt tgccttgcgc cgggaggcgg gtctagccaa
     5881 gctgcgcgag ctctacgcct cgtggggtta cgcccccgtc gaggtgccgg tgctcgagcg
     5941 cttcgacccg taccacccgc gcgaggagca agcgttcaag ctgatcgaca acggcagcga
     6001 ggtcatcgcc ctgcgctcgg acttcacgcc agcgctcgcc aggctcgtgg cgatcacgca
     6061 ccctggggtg gccgccggcg agaggaggcc gctgcggttc cggtacgcgg gcaccgtctg
     6121 gcacgccaac catcccgagg tggctggcat gcgcgagttc acccaggtcg gcatcgagct
     6181 cgtcggtgtg agcaacgcgc gcgccgacgc cgagatcatc cacctggcac gcgagtccgt
     6241 gcgggcgctt ggcctcgccc cgcgcatcga ggtaggcaac cctgcctacg tgaaggccct
     6301 gttggaggcg gcgcaggtcc cgcgcgagca ccagggcgcc ctcgccgacg ccatcgaccg
     6361 caaggaccgc tccgacgtcc tggccgcgat cgaggccctc gggttgcgtg gcaaggcggc
     6421 cgacgccatc ctccaggcgc ccgacctcta cggggcggag agcgtcctca tcgaggcccg
     6481 gcgcttggct cccgacgcag ctgccaacct agccctcgac cggctggaag gtgtgctcgc
     6541 cgagttcgag gacgctagcg agctcctgct cgatctcggc ctggcgcggc gcctcagcta
     6601 ctacacgggc gtgacgttcc gcgcgttcac gccggactac ggccggccgc tgctgggcgg
     6661 cggacgctac gacgccgcgc tgctgcctta cgccgccggg ttctcgatgg gtctcgaacg
     6721 cctcctgggc gcggc
//
LOCUS       DAOQYE010000009         6729 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_11725_length_6729_cov_3.606083, whole genome shotgun sequence.
ACCESSION   DAOQYE010000009 DAOQYE010000000
VERSION     DAOQYE010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 6729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6729
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11725_length_6729_cov_3.606083"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..937)
                     /locus_tag="PKN52_00500"
     CDS             complement(<1..937)
                     /locus_tag="PKN52_00500"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HNQ98442.1"
                     /translation="MKQPVRVAVTGAAGQIGYALLFRIAAGDMLGKDQPVILQLLEIT
                     PALKALAGVVMELDDCAFPLLHGVVATDDPNVAFKDADYALLVGSRPRGPGMERKDLL
                     QANGAIFTVQGKALNDHASRGVKVLVVGNPANTNCLIALKNAPDLAPEQFSAMTRLDH
                     NRALSQLAAHTGAKVSDVRKMIIWGNHSSTQVPDVSHATVAGRPAASLVDDAWVDGEF
                     VPTVQKRGAAIIDARGASSAASAANAAVDHMRTCALGSPEGDLVSMGVLSSGAYDVAE
                     DIVYSYPVTVRDGVVAVVEGLSVNATVRSKMRATEQ"
     gene            complement(1192..2016)
                     /locus_tag="PKN52_00505"
     CDS             complement(1192..2016)
                     /locus_tag="PKN52_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HNQ98443.1"
                     /translation="MSAKPSRGLGRGLDALLPKVEKGGVQQLLVAQLRPSPFQPRQKM
                     DEAGIAEMADSIARKGVLQPIVVRPVDGGYEIVAGERRFRAAQRAGLTSVPATVRELS
                     DQETLEIAVVENLQREDLNSMEEARAFKLLLDFGMNQERVSEAVGKSRSAIANALRLL
                     ALPEEAQAAVEAGAISAGHARAILAQPEPDRLWALSEITRRDLTVREAESLKRPTARS
                     AQRKAAGRYARLEEDLSRFAGTRVRISGNARKGRIELHFHSEDELQRLLDLLGYES"
     gene            complement(2000..2758)
                     /locus_tag="PKN52_00510"
     CDS             complement(2000..2758)
                     /locus_tag="PKN52_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNQ98444.1"
                     /translation="MPIIGVINQKGGVGKTTTAVNLSAALAERRRILLVDLDPQANAT
                     SGVGVGAPELTLYDVIAGRASARQAVIATGTENLHVLAASADLAGVQVEVDAGRENMR
                     LLARGLMGVRPNYDFIIVDAPPSMGVLTLNALAAADLLIIPLQSEYYALEGIASMMDT
                     VERVRGSVNPDLRILGIALTMYDSRTRLSQEVEDNVRKHFGDLVFRTIIPRTVRLAEA
                     PSYGRSVLEYAPTSQGALAYRELAEEVIQRVSEA"
     gene            complement(2740..3327)
                     /locus_tag="PKN52_00515"
     CDS             complement(2740..3327)
                     /locus_tag="PKN52_00515"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNQ98445.1"
                     /translation="MNDAGIALQQYRDLVVRYHTTLDLMSDRGVADLDRFIAEGRRFA
                     ALVERLAGPTATVVDVGSGAGLPGIVIAACLPGSLVHLVERRRRRVAFLELAAAALGL
                     ANARVFGEDVRTMTGVTADVVTAQAVTGLAELAGLASSVMTDATWLLTRRGEGWRADA
                     EGITAGAEVVEEPLEHRGSLVALKLLGGAACRSSA"
     gene            complement(3727..5568)
                     /gene="mnmG"
                     /locus_tag="PKN52_00520"
     CDS             complement(3727..5568)
                     /gene="mnmG"
                     /locus_tag="PKN52_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HNQ98446.1"
                     /translation="MRQEFDVVVVGGGHAGIEAAIAAARLGARAALVIPNPDKIGVMP
                     CNPAIGGPGKSQLVFELHALGGVMGRLADETAIHTRMLNASKGAAVQSLRVQNERDGY
                     AAAARALVESEPLVTIVRAEVAELLAAAGSVTGVMTTDGRTLAAPAVVLCTGTFLAGV
                     VWYGKRQRPAGRQGEAPARHLSASLRTTGHDLVRLKTGTPPRIRADSVDLSVLQEVPS
                     DDPPGTFSGVPGPRMTSTPTWLTRTTPETHALIQEHLLESAMYGGEIDATGPRYCPSI
                     EDKVVRFSDKDHHLLFVEPDGLDTSELYLQGLSSSLPPNIQDELILTLPGFERAVIHR
                     YAYAVEYDALDPAQLDVTLMSRKLAGLFSAGQINGTSGYEEAAAQGLIAGVNAARHAA
                     GQVRVTVRRDQGYIGVMLDDLVRWGIAEPYRMMTSRNEHRLLHRQDNASERLMALGHE
                     WGLVSGQALERQRASEARVNREVSRLGRVRHGGDLAKTILCRPGVEYGDVVALVGAAA
                     DELAPGEVEQVVTQVRYASYIERAKLQRDGQAAYERMSLEGVDFGVVASLSTEGLQAL
                     RRAAPASLGAAQRVRGVRDSDVAALLVHLKVHRHGGTGARGTGAPGA"
     gene            6188..>6729
                     /locus_tag="PKN52_00525"
     CDS             6188..>6729
                     /locus_tag="PKN52_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA/Hda family protein"
                     /protein_id="HNQ98447.1"
                     /translation="MAGKAQGMWDDVIARIRAEIPEVEFRTWFSQVTPQGIEGGAFVL
                     GVPHSFARDWLKSNYGDVIETALRELGVEPPRVAFQVTGSKVIEQQDMFSTPPTPETR
                     AESPEVRRPKLNPKYVFSHFVVGPNNNLAHAAARAVVEAPGKAYNPLFLYGESGLGKT
                     HLMHAVGHAVFESRPDLQIE"
BASE COUNT          986 a         2370 c         2260 g         1113 t
ORIGIN      
        1 cctgctcggt ggccctcatc ttgcttcgca ccgtggcgtt caccgacagc ccttcgacca
       61 ccgcaacgac gccgtcccgc accgtgaccg ggtaggagta gacgatgtcc tctgccacgt
      121 cgtaggcgcc actgctcaag acccccatgc tgaccaagtc gccttcgggg ctgccaaggg
      181 cacaggtgcg catgtggtcc acggcggcgt tggccgccga agcggcgctg gacgcgccgc
      241 gggcgtcgat gatggccgcg ccgcgcttct ggacggtcgg gacgaactct ccatccaccc
      301 aagcgtcgtc caccaagctc gcggccggtc tgccggccac ggtcgcgtgg ctgacgtctg
      361 gcacctgggt gctggagtgg ttgccccaga tgatcatctt gcgtacgtcg gataccttcg
      421 cgcccgtgtg cgcagccagt tggctcagcg cacggttgtg atccagtcgg gtcatcgccg
      481 agaactgctc cggagccagg tccggggcgt tcttcaaggc gatcaggcag ttggtgttgg
      541 ccgggttgcc aaccaccagg accttcacgc cgcgcgaagc gtggtcgttg agcgccttgc
      601 cttgcacggt gaagatcgcg ccgttcgctt gcagcaggtc cttgcgctcc atcccggggc
      661 cccggggccg ggagccgacc aggagggcgt aatcggcgtc cttgaacgcg acgttcgggt
      721 cgtcggtcgc gacgaccccg tgcagcagcg ggaaggcgca gtcgtccaac tccatgacga
      781 cgcccgccag ggccttcagg gccggcgtga tctcgaggag ttgcaggatc acgggctggt
      841 ctttgccaag catgtcgcct gccgcgatac ggaacaggag tgcgtaaccg atctggcctg
      901 cggccccggt gaccgccacg cgtacgggtt gcttcatcgc ttcacctcgg ttcggtctca
      961 gcccggcatg ctgcgacgca gatggcccgg gcacggttgc atgtctcatg cggttggcaa
     1021 gcgccccgat gcgtgcggcg ctacgactcc aacgtcggcg ccatcgtatc accgggtcgg
     1081 gccgccgctc gcgccaggag cgcgaatcgg tggccagggg tggggcggtg gacgggcacg
     1141 gacccggggt cggccagggg tgcgcgcccg ggcgcgtctg gggcgcgccc gctagctctc
     1201 gtagccgagc aggtccagga ggcgctggag ttcgtcctcg ctatggaagt gcagctctat
     1261 gcgacccttc ctcgcgttgc cggagatccg gacgcgcgtg ccggcgaagc ggctcaggtc
     1321 ctcctccagg cgcgcgtagc gccccgcggc ctttcgttgc gccgacctcg cggtggggcg
     1381 tttcaggctt tcggcctcgc gaacggtcag gtcgcggcgc gtgatctcgc ttaaggccca
     1441 cagccggtcc ggctcgggtt gggccaagat ggccctggcg tgaccggcag agatcgcgcc
     1501 ggcctcgacg gccgcctggg cctcctcggg cagcgcgagc aacctgagcg cgttggcgat
     1561 ggcgctcctg ctcttgccga cggcctcgga cacgcgctcc tggttcatac cgaagtccag
     1621 caggagcttg aacgcccgcg cctcttccat ggagttgagg tcctcgcgct gcaggttctc
     1681 gacgacggct atctcgagag tctcctggtc gctcagctcc cgcacggtcg ccggcacact
     1741 ggtcagtccg gcccgctgcg cggcccggaa tcgcctctcg ccggcgacga tctcgtagcc
     1801 gccgtcgacg ggccgcacca cgatgggctg gagcacaccc ttgcgggcga tggagtcggc
     1861 catctccgct atgccggcct cgtccatctt ctgtctcggc tggaagggcg atggtcgtag
     1921 ttgcgcgacc agcagctgtt gtacgccgcc cttctccacc ttcggcagca gtgcgtccag
     1981 ccctcggcca agacccctac taggcttcgc tgacacgctg gatgacctcc tcggccagtt
     2041 ctcggtaggc taacgcgccc tgcgaggtgg gtgcgtactc gagcacggag cgcccgtagg
     2101 aaggcgcctc ggccaaccgc actgtacgcg ggatgatggt cctgaacacc aggtcgccga
     2161 agtgcttcct gacgttgtcc tctacctctt gagacagccg cgtccggctg tcgtacatgg
     2221 tcagcgcgat gccgaggatc cgcaggtccg ggttgaccga gccccggacg cgttcgacgg
     2281 tgtccatcat ggacgcgatc ccttcgagcg cgtagtactc gctctgcagc gggatgatca
     2341 gaaggtcggc ggccgcgagc gcgttgagcg tcagaacgcc catggaaggc ggcgcgtcga
     2401 cgatgatgaa gtcgtagttg ggccgcacgc ccatcaaccc ccgggccaga agtcgcatgt
     2461 tctcgcggcc cgcgtccacc tcgacctgca ctccggccag atcggcggag gcggcgagca
     2521 cgtggaggtt ctccgtgcct gtggctatca ccgcttgccg cgccgaagcc cgcccggcta
     2581 tgacgtcgta cagcgtcaac tcgggcgcgc cgacgccgac gccgctcgtc gcgttggcct
     2641 ggggatccag gtcgactaga aggatgcgcc gccgctccgc gagggcggcc gacaggttga
     2701 ccgcggtcgt ggtcttgcca acacccccct tctggttgat cacgccgatg atcggcacgc
     2761 cgcgcctccg aggagcttca gtgcgacgag gctaccacgg tgttccaggg gctcctcgac
     2821 cacctcggct ccagccgtga tgccctcggc atcggcgcgc cagccttcgc cccgacgcgt
     2881 cagcagccag gtcgcgtcag tcatgacgct cgacgccaac cctgcgagct ccgcaaggcc
     2941 ggtgacggct tgggcggtga ccacatcggc ggtcacgccg gtcatggtgc gcacgtcctc
     3001 cccgaagacc ctggcgttag ccaggccgag cgcggcggcg gccaactcca agaacgccac
     3061 tcgacgccta cggcgctcga cgagatggac gagcgacccc ggtaggcagg cggcgatcac
     3121 gatgccgggt agcccggccc cggagccaac gtccacgacc gtcgcggtag ggccggcaag
     3181 gcgctccacc agggccgcga accgccgccc ttcggctatg aagcggtcca ggtccgcgac
     3241 tccacggtcc gacatcaggt cgagggtggt gtggtagcgg acgacgaggt cgcggtactg
     3301 ctggagcgcg atgccggcat cgttcatgcg atcttccccg gtcctgacgt cgcccgcggg
     3361 tccgcggacg ggcgggtgtc tgcgtcgtcg ctacgtccgc cgaagcggat gaatgccttc
     3421 ctggacggtc taattcgcga ttgtttcacg tgaaacactt ccccgagatc cgcttggccg
     3481 acaggtcacc ggtcgtagcc ggtcgttggc gctcgccggc gcgcgctcac cagaagccga
     3541 gtggtgctta gggtgaccgc atgcccgtgt cgctcgccgc acgactgccg agccgcaagc
     3601 gcgggctccc cgtatcgggg ccccgatacg gggcccgtac ccaggccccg gcacggggtc
     3661 gccagcgccg tcgcacgagc cggagcttac cgcgcgcggc cggacgcata tccggccctc
     3721 agcggctcag gccccaggcg ccccggtccc gcgtgcccca gtccctccgt gcctatgcac
     3781 cttcaggtga acgagcagtg ccgccacgtc gctgtctcgc acgcctcgca cgcgttgagc
     3841 ggccccaagg gaggcgggcg ccgcgcggcg gagggcttgg aggccttcgg tcgaaaggct
     3901 cgccacgaca ccgaagtcga cgccttccag gctcatacgt tcgtatgcgg cttgtccgtc
     3961 gcgctgcagc ttggcgcgct cgatatatga ggcgtacctg acctgtgtta cgacctgttc
     4021 gacctcgcct ggcgcaagct cgtcggcggc cgctcccacc aacgcgacga cgtccccgta
     4081 ctccacgccg ggccggcaaa ggatggtctt ggcgagatcg cccccatggc ggacgcgtcc
     4141 caggcgcgac acttcgcggt tcacgcgcgc ctcggacgca cgctgccgct cgagggcctg
     4201 cccggacacg agcccccact cgtgccctag cgccatcaag cgctcgctgg cgttgtcctg
     4261 acgatggagg agccgatgct cgttgcgcga cgtcatcatc ctgtacggct ccgcgatccc
     4321 ccagcgcacg aggtcgtcga gcatcacccc gatgtagccc tggtccctgc ggaccgtcac
     4381 cctgacctga ccggccgcgt ggcgcgcggc attgactccg gctatgagtc cctgcgcggc
     4441 ggcctcctcg taaccgctcg taccgttgat ctgaccagcc gagaacagtc ccgccagctt
     4501 gcgcgacatg agggtcacgt cgagctgcgc agggtcaagg gcgtcgtact ccacggcgta
     4561 ggcgtaccgg tgaatcacgg cccgctcgaa cccgggcagc gtcaggatca gttcgtcctg
     4621 gatgttcggc ggcaacgagc ttgagaggcc ctggaggtag agctcgctgg tatccaaccc
     4681 gtccggctcg acgaacagca ggtgatggtc cttatcggag aagcggacga ccttgtcttc
     4741 gatggagggg cagtagcggg gtccggtcgc gtcgatctcg cccccataca tcgcggactc
     4801 gagaaggtgt tcctggatca gcgcatgcgt ctcgggggtc gtgcgcgtta gccacgtcgg
     4861 tgtgctcgtc atgcgcgggc cgggcacgcc gctgaaggtg cctggcgggt cgtcggacgg
     4921 cacctcctgc aagacactca ggtcgaccga gtccgcccgg atgcgcggcg gcgtgccggt
     4981 cttcaggcga acgaggtcgt ggcccgtggt tcggagcgac gccgacaggt gccgcgccgg
     5041 cgcctcgcct tgccggcctg ccggccgctg cctcttcccg taccagacga cccccgccag
     5101 gaaggtgccc gtacagagga ctaccgcggg cgcagccagc gttcgaccgt ccgtcgtcat
     5161 caccccggtc acggatccgg cggcggcgag cagttccgcc acctcggccc gcactatcgt
     5221 cacgagcggc tcgctctcga ccagggccct ggcagctgct gcgtatccgt cgcgttcgtt
     5281 ctgaacccgc agtgactgca cagccgcccc tttcgaggcg ttgagcatgc gcgtatgtat
     5341 ggccgtctcg tccgccaagc ggcccatgac gccgccgagg gcgtgcagtt cgaacacgag
     5401 ctgcgacttg ccaggtcccc cgatcgcggg gttgcacggc atgacgccga tcttgtcagg
     5461 gttggggatg accagcgccg cccgcgcccc gaggcgagcc gcggcgatgg ccgcctcgat
     5521 gcccgcatgc cctcccccta cgacgaccac gtcgaactct tgtcgcatcc tgccctccct
     5581 cgaccgaggt atgccgtgcg gggcagcccg gacggccgcg ccccgcggtt ctgcagcgct
     5641 cggttccatg gcgctcggtt tcgcaccact ctgcaagcgc cgagcaagcg cccacgagta
     5701 tagcgggaag gcctcgctct cgcggttgca aggccagcgc acgccaggct ccgtcgcgag
     5761 gagcgtctgg tacactgcct cggtcaacgg cgcctgcggt ggaacccgag catgagcacg
     5821 gtccaaggcg ggcacgggct accaggccgg caccgtcaat cggacgagtt cgctgtgaag
     5881 cagggccgcc gcgcaccgca ccgggtcgct agccgcgctc ggctgccgtt ggcggtcagt
     5941 tcagcgctct cgtccgctcg gagctagggc cggctcggta cctcggttcg ctcgaggcta
     6001 cggctcactc ggcggttcgc ttggcggccc gcttagcggt tcgctcggcg caccggcact
     6061 ctcggcactt ggcgaggcac cctgcctagt cctcggggcg cgtcctcggg gagccgactt
     6121 gcccccgagt ggcgggtgat gccagaatcg gcgtctcgca ggaccagtag gaaggggaac
     6181 gttcacgatg gcgggcaagg cacaggggat gtgggacgac gtcatcgcgc gcatccgcgc
     6241 cgagatcccc gaggtcgagt tccgcacgtg gttcagccag gtgaccccgc aaggtatcga
     6301 ggggggcgcg ttcgtcctcg gagtacccca ctccttcgcc cgcgattggc tcaagtccaa
     6361 ctatggcgac gtgatcgaga cggcgctgcg cgagctaggc gtcgagcccc cacgagtcgc
     6421 gttccaggta accggcagta aggtcataga gcagcaggac atgttcagca cgccgcctac
     6481 gccggagacg cgcgcggagt cgcccgaggt ccgtcgcccc aagctgaacc ccaaatacgt
     6541 cttctcgcac ttcgtggtcg gacccaacaa caacctcgct cacgccgcgg cgcgcgcggt
     6601 ggtcgaggcc cccggcaagg cgtacaaccc cctcttcctg tacggggagt ccggccttgg
     6661 caagacccat ctcatgcacg ccgttggaca cgcggtcttc gagtcccggc cggacctcca
     6721 gatcgagta
//
LOCUS       DAOQYE010000010         6694 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_11800_length_6694_cov_2.822262, whole genome shotgun sequence.
ACCESSION   DAOQYE010000010 DAOQYE010000000
VERSION     DAOQYE010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 6694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6694
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11800_length_6694_cov_2.822262"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="PKN52_00530"
     CDS             <1..376
                     /locus_tag="PKN52_00530"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNQ98448.1"
                     /translation="RFLGRVPRERVGEWLSECDAFVQPSLYEGLSLAVLEAMARGAPV
                     LVSDIPANRDPIEAPDGPVGWCLPGNYPRAWAAVLHEVAADPVAAAALGERGRARQRA
                     EFDEETMYGRYVQLVLEELGGR"
     gene            397..792
                     /locus_tag="PKN52_00535"
     CDS             397..792
                     /locus_tag="PKN52_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98449.1"
                     /translation="MVFALLVGIVALVADRVPSLANAVPPPRLAWAFIPQGVPEGAPF
                     AAWFGFDAAAAPSGLPAAVQLRVCDLEGACLLGARKAVTERSSSGLVTITRALPPGEY
                     DLHLLVLRPNRLGVTRTVQVVSRRVTYGG"
     gene            782..1276
                     /locus_tag="PKN52_00540"
     CDS             782..1276
                     /locus_tag="PKN52_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98450.1"
                     /translation="MAVSRGSALPSTSGAWAILAIVCLCVVATGLATGRVSQLTAPSV
                     AAPPSMPQTAFGACGEPAFIVGSGEAAGGELIAFTNALVLVPGCEAAGRLLLTGSLAR
                     GVGPAVSVTYVGAGGESTGWYGEVDTGGVEVPLPPATRALVSFTNDLASGAEDRNLRL
                     EVAP"
     gene            1276..2346
                     /locus_tag="PKN52_00545"
     CDS             1276..2346
                     /locus_tag="PKN52_00545"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNQ98451.1"
                     /translation="MGRALRNVALSALLGVLIVSVLRGRPWLLGWAALILVLARSTLA
                     RRPRSARVAYRLTPCLAIAAALGLALQALGVAVHGDLGRSAGFLPHANTAAATTVALW
                     TLLAVCSAMMPSRGGRWWTIGRGLLVFGSCLSGLLLLAAGSRSLMFGLLFGIVVAAAA
                     RKPRTLGWRAWLPLGAAVATLGVGLLVSARLRPDVDSVLGSFERGPIFMAALEIAALS
                     PWTGLGDGAWTRWAPSVEPSLPLAAAVHTHSVPLEALVGGGAIGLAALLVLLVLGARS
                     LLARRAGDGALAGSALIVGVVALAVQGLVDLAVLYPGVYAPLALAMASWDVLSDVRPE
                     ARKERGHGEQGVERGSSGAVSR"
     gene            2363..3145
                     /locus_tag="PKN52_00550"
     CDS             2363..3145
                     /locus_tag="PKN52_00550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HNQ98452.1"
                     /translation="MRISPLTLDQWLAVVADGVESRTRRLLVSQNMHSAYLAVNSPAL
                     TALQARADVVRIDGMPLVWIARLLGLPVTRAQRAGFMDLMPVLMSAAAERGWKVAVIA
                     GRPGVAERAATRLRSEHPGLELVTEDGYFALDDSDGEVSRRLERVAAERADVVLIGMG
                     MPRQEEFLQRYLDRIAAPVVGTCGAAFDYVAGAIPMAPRWLSRLGLEWLFRLVAEPRR
                     LWRRYLVEPLGLLPRLTADLRKRRLGETHFRFDTVENGRGAA"
     gene            3142..4011
                     /locus_tag="PKN52_00555"
     CDS             3142..4011
                     /locus_tag="PKN52_00555"
                     /inference="COORDINATES: protein motif:HMM:NF025663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp fold amidoligase family protein"
                     /protein_id="HNQ98453.1"
                     /translation="MRRTLLSVAARVLTPAAFARLHCAAKLGYRPNLDNPTTFTERLL
                     AKRVFDHDPLIPMTADKFTLRGYVEERLGADYLPRLYAVIESAADVDLTSLPADYVMK
                     GTHGSGWNRIVTGSDLTRAEAMTLATDWLGRSYYRKRLEWAYRDLTPRVVFEENLAPG
                     RPIDDYKVFVFGGVPRIIQVDRDRFTDHRRTLYTPEWRAVSVACEYPLLAAPVEVPPR
                     LGEMLGAAAKLAEPFAFARVDFYSLPDRLVVGEITHYPEGGVVRFEPRAFDLELGAVW
                     GGGRPVGGEFLTS"
     gene            4008..5273
                     /locus_tag="PKN52_00560"
     CDS             4008..5273
                     /locus_tag="PKN52_00560"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HNQ98454.1"
                     /translation="MKRGMRDVMQLMTGTAAGQAIGFLAAPLLSRLYTPDDFGVVGVF
                     LSVATVLGVVAALRLELAVVVPKADEDAQHVMAAAMLVVAGVVIGTTLAVLFAGAAAS
                     RALGAPALVQLLGILPFYVGSLGTFQVFNYWSTRVGKFGRLASAQVTRGVVGAAGQIG
                     SGAAHLGAPGMLAGQVAGQVAGTLVLLGRSARAGRLDLRAPVSFARSGKVLREYADFI
                     VYGAPQSLLNAIGQGLPAIVLTATFGAGAAGQYLLARRVVTAPSNLLGQSLRQVIYPH
                     LSRQIDRQAVLPFVVKATLALFAIAAVPAALLAVFGPRAFAWGLGDQWRIGGEFARYL
                     GLNLIGGLMNIPAVSLVPLLRIQRWHAVYEAIYTLARVGALVGGGMVAGPVGAVAGMA
                     FVGIAFNVFLIIAVVARLKARLASRAAQE"
     gene            5297..6181
                     /locus_tag="PKN52_00565"
     CDS             5297..6181
                     /locus_tag="PKN52_00565"
                     /inference="COORDINATES: protein motif:HMM:NF025663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp fold amidoligase family protein"
                     /protein_id="HNQ98455.1"
                     /translation="MGKLVDSLKALLPDRLHTDMQYLWRFGRLRNHRDPKTFHELLIR
                     KKVLDVARYRELGVLSDKYGVREFVERVVGPEYLIPLIGVYDSVDDIDAEALPNAFVL
                     KGTHGSGMNLIVRDKARVDWDEFRAAARRWLATDYSRLYREAPYRAVPRRLVAEELLS
                     GVDGAVPVDYKFFVLSGKVRLLEADYSRFTRHMQAFFLPDGRRLAVDRGRAVPELLPP
                     LPTQLAEMVDLAEKLGAGHEYIRVDLYDVGGRTYFGELTFYPSGALNLWRPAQFEYEM
                     GAVWREGRPIGEEWVAGG"
     gene            complement(6189..>6694)
                     /locus_tag="PKN52_00570"
     CDS             complement(6189..>6694)
                     /locus_tag="PKN52_00570"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HNQ98456.1"
                     /translation="FTVNIALIEDGVPVLGVVHAPVAGLTYTGLASHAGKPASAWRFD
                     AEGGAHPIHVRRPTAGEPVRVMVSRSHSNPSTAAFIERLRAEYGEVQTIARGSAIKTC
                     LVADGTAHFYARLGPTMWWDTAAAQAVLEAAGGSMVEASGGRALRYVGDALSNPGFVA
                     ASEASWA"
BASE COUNT          869 a         2251 c         2398 g         1176 t
ORIGIN      
        1 acggttcctc ggacgcgtgc cgcgcgaacg cgtcggcgag tggctctcgg agtgcgacgc
       61 cttcgttcag ccgtcgctct acgaaggcct gagcttggcc gtgctcgagg ccatggcgcg
      121 cggggcgccg gtcctcgtca gcgatatccc cgccaaccgc gatccgatcg aggcccccga
      181 cggcccggtc ggctggtgcc tgccggggaa ctatccgcgc gcttgggcgg cggtgcttca
      241 cgaggtggct gccgatccgg tcgcggccgc tgcgctgggt gagcggggcc gcgccaggca
      301 gagggcggag ttcgacgagg agaccatgta cggcaggtac gtgcagttgg tcctcgagga
      361 gctgggcggc cggtgaggag ctacttgact gcgtccgtgg tattcgccct cctggtcggc
      421 attgtcgctc tcgtcgccga ccgcgttccc agcctggcga acgccgtccc gcccccccgt
      481 ctggcgtggg ctttcatccc gcagggcgtg cccgaaggcg ctcccttcgc cgcgtggttc
      541 ggtttcgatg ccgccgccgc gccttccggg ctgccggcag ccgtgcaact gcgcgtctgc
      601 gacctggagg gggcgtgcct gctcggggcc cgcaaggccg tcaccgagcg ctcgagcagc
      661 ggcctggtga cgatcacgcg cgcgctgccg ccgggagagt acgacttgca cctgctggtc
      721 ttgcgtccga accggttggg ggtcacccgc acggtccagg tggtgtcccg gagggtgacg
      781 tatggcggtt agccgcggct ccgccctgcc gtccacctcg ggcgcttggg ccatcctcgc
      841 gatcgtctgc ctctgcgtgg tcgcgaccgg tctcgccacg ggtagggtga gtcagctgac
      901 cgctccatcc gtggccgcgc cgccctcgat gccacagacc gccttcggag cctgtggcga
      961 gcctgccttc atcgtggggt caggtgaggc cgcgggcggc gagttgatag ccttcaccaa
     1021 cgccctcgtc ctggtgcccg gttgcgaggc cgcagggagg ttgctcctca cgggcagcct
     1081 ggcgcgaggg gtgggccccg cggtgagcgt cacctacgta ggcgccggtg gagagtcgac
     1141 cggctggtac ggcgaggtcg acaccggagg cgtcgaggtg cccctcccgc cggcgacgcg
     1201 ggcgctcgtg tcgttcacca acgacctggc cagcggagca gaggaccgga acctcaggct
     1261 ggaggtagcg ccttgatggg ccgggctctc aggaacgtgg ccctcagcgc cctcctgggg
     1321 gtcttgatcg tgtcggtctt acgaggacga ccgtggctcc tcggctgggc cgcgctcatc
     1381 ctcgtcttgg cgaggtcgac cttggcgcgg cgaccccgtt cggcgcgggt cgcctaccgg
     1441 ctgaccccat gcttggccat cgccgcagcg ctcggcctgg cactgcaggc gctgggtgtg
     1501 gctgtccatg gcgacctggg tcgatctgcc gggttcttgc cgcacgccaa cacggcggct
     1561 gcgacgacgg tggccctatg gaccttgctg gcagtctgct ccgccatgat gccgagccgg
     1621 ggcggtaggt ggtggaccat cggacgtggc ctgctcgtct tcgggagctg cttgagcggg
     1681 ctactcttgt tggccgccgg cagccggtca ctgatgttcg gcttgctctt cggcatcgtg
     1741 gtagcggcgg cggctcgcaa gcctcgcacc ttgggttggc gcgcctggct cccgctcgga
     1801 gcggccgtcg ccacccttgg tgtcggactg ctcgtctcgg cgcgcctacg ccccgacgtc
     1861 gactccgtgc tcggctcgtt cgaacgcggc cccatcttca tggccgcgct tgagatcgcc
     1921 gctctctcgc cctggaccgg cttgggggac ggcgcatgga cccgttgggc gccctcggtg
     1981 gaaccgtcgc tgcccctggc ggcggctgtc cacacgcata gcgtgccgct cgaggcgctg
     2041 gtgggcggag gagcgatagg ccttgcggcc ctcctcgtgc tgctggtgct tggggcgcgc
     2101 tcgctcctcg cgcgccgggc gggcgacggc gccctcgccg gtagcgcgct gatcgtcggc
     2161 gtcgtcgccc tggcggtcca ggggctcgtg gacctggcag tcttgtaccc tggcgtgtac
     2221 gctccgttgg cgctggccat ggctagctgg gacgtactgt ctgacgtgcg gcctgaagcg
     2281 cggaaggaac gaggacacgg tgagcaaggg gtcgagcgag gatcgtcagg ggccgtctcg
     2341 cgttgattac agggttctcg gggtgcggat ctccccgctg acgctcgacc agtggctcgc
     2401 cgtcgtcgcg gatggcgtgg agagccgcac gaggcgtctc ctggtcagtc agaacatgca
     2461 tagcgcctac ctcgccgtga acagccccgc cctgacggcc ctccaggcgc gcgccgacgt
     2521 ggtcaggata gacggcatgc cgctcgtatg gatcgccagg ctgctcggcc tccccgtcac
     2581 gcgggcgcag agggctggtt tcatggacct gatgcctgtc ctcatgtcgg cggccgcaga
     2641 gcgcggttgg aaggtcgcgg tcatagcagg tcggcctgga gtggcagagc gggcggccac
     2701 gaggctgcgc tcggagcatc cggggctgga gttggtcacc gaagacggct acttcgctct
     2761 cgacgactcg gacggcgaag tctccaggcg ccttgagcgg gtggccgccg agcgcgccga
     2821 cgtcgtgctc atcggcatgg gcatgccccg gcaggaggag ttcctacagc gctacctcga
     2881 ccggatcgca gcaccggtcg tcggcacgtg cggagccgct ttcgattacg tggcgggcgc
     2941 catccccatg gcgccgcgtt ggctctcccg cctcggtctc gaatggctct tccgactggt
     3001 ggccgagccc cgcaggctct ggcggcgtta cctcgtcgag cccttgggcc tgttgccgag
     3061 gctgacggct gacctgcgca agcggcgcct gggagagacc cacttccggt tcgacaccgt
     3121 cgagaacggt cgcggcgcag cgtgaggcgg acgctcctat ccgtcgccgc tcgggtgttg
     3181 actccggcgg cgttcgcgcg actgcactgc gctgccaagt tgggttaccg gccgaacctc
     3241 gacaacccca cgaccttcac ggagcggctc ttggccaagc gcgtgttcga ccacgacccc
     3301 ctgatcccca tgacggccga caagttcacg ttgcgggggt acgtggagga gagactgggc
     3361 gccgactacc tgccgcgcct ctacgctgtc atcgagtctg ccgcggacgt cgacctgacg
     3421 agcctgcccg ccgactacgt gatgaagggt acgcacggta gcggctggaa ccgcatagtg
     3481 accggctccg acctgacgcg ggccgaggcc atgaccctgg ccacggactg gctggggcgc
     3541 tcttactacc ggaagcggct cgaatgggcc taccgcgacc tgacgccgcg ggtggtgttc
     3601 gaggagaacc tggcgcccgg cagacccatc gacgactaca aggtgttcgt gttcggtggg
     3661 gtgccgcgca ttattcaggt cgaccgcgac cggttcactg accaccgccg caccctttac
     3721 acgcccgagt ggcgagcggt ctccgtggcc tgcgagtacc cgctcctcgc cgcgccggtg
     3781 gaggttcccc cccgtctcgg ggagatgctc ggagcggccg ccaagttggc cgaaccgttc
     3841 gccttcgctc gagtcgactt ctatagcctc cccgaccgcc tggtggtcgg ggagatcacg
     3901 cactatccgg aagggggcgt ggtgcgcttc gagccgcggg cgttcgactt ggaactcggc
     3961 gccgtgtggg gcggcggcag gcccgtcggt ggagagttcc tgacatcgtg aagcgcggca
     4021 tgcgcgacgt gatgcaactg atgaccggca cggctgccgg tcaggccatc ggtttcctcg
     4081 ccgcgccgct gctcagtcgc ctctacacac ctgacgattt cggggtcgtc ggcgtgttcc
     4141 tctccgttgc cacggtcctc ggggtcgtgg cggctttacg cctcgagctc gcggtcgtcg
     4201 ttcccaaggc ggacgaggac gcccaacacg tgatggcagc cgccatgctc gtcgtggcgg
     4261 gcgtcgtgat cggcacgacg ctggcagtcc tgttcgcggg ggcggccgcc tcgcgcgccc
     4321 ttggcgcccc cgcgctcgtt cagctcctcg gtatcctgcc cttctacgta ggttcgctgg
     4381 ggaccttcca ggtgttcaac tattggtcga cgcgcgtcgg caagttcggg cgcctcgcga
     4441 gcgcccaggt gacacgcggc gtggtgggcg cggcgggaca gatcggatcg ggcgccgcgc
     4501 acttgggtgc gcccggcatg ttggccgggc aggtggcggg gcaggtggcg gggacgctcg
     4561 tgctgctcgg cagatcggcg cgcgcggggc gcctcgacct acgcgctccc gtgagcttcg
     4621 cgcgctcggg caaggtgctg agagagtacg cggacttcat cgtctacggc gccccacagt
     4681 cgctactcaa cgccatcggg cagggcctgc ccgcgatcgt tctcacggcg accttcgggg
     4741 cgggggccgc gggccagtac cttctcgcca ggcgcgtcgt cacggctccc tccaacctgc
     4801 tgggccagtc gttacgccag gtcatctacc cgcacctcag caggcagatc gaccggcagg
     4861 cggtgctgcc gttcgtcgtc aaggcgaccc tggccttgtt cgccatcgcg gccgtgccgg
     4921 ccgcgttgct cgccgtcttc ggccctcgcg ctttcgcctg gggtcttggc gaccagtggc
     4981 ggatcggcgg cgagttcgcc agatacttgg gcctgaacct aatcggcgga ctcatgaaca
     5041 tccccgccgt cagcctggtc cccttgctca ggatccagcg ttggcatgcc gtctacgagg
     5101 cgatctacac gttggcccgc gtcggggcgc tcgtgggtgg cggcatggtg gcggggccgg
     5161 tcggcgccgt ggcgggcatg gccttcgtcg gcatagcgtt caacgtgttt ctgataatcg
     5221 ctgtcgtagc gcggctcaaa gcgcgcctgg cctcccgcgc cgcccaggag tagggtcggc
     5281 tatcatctgg tctcgcatgg gaaagctcgt ggacagtctg aaagccttgc tgcccgaccg
     5341 cttgcacacg gacatgcagt acctctggcg tttcggacgc ctcaggaacc accgcgaccc
     5401 caagaccttc cacgagttgc tgataaggaa gaaggtattg gacgtcgctc ggtaccgcga
     5461 gctaggcgtg ctgtccgaca agtacggagt gcgtgagttc gtggagcggg tcgtcggccc
     5521 ggagtacctg atccctctga taggcgtata cgacagcgtg gacgatatcg atgccgaagc
     5581 cctgccgaac gccttcgtgc tgaagggtac ccacggctcc ggcatgaacc tgatcgtcag
     5641 ggacaaggcg cgcgtagatt gggacgagtt ccgggctgct gcgcggcggt ggctcgccac
     5701 cgactactcg cgactgtatc gcgaggcgcc ttatagagcg gtgccgcgca ggctggtggc
     5761 agaggaactg ttgtccggcg tggacggggc ggtccccgtc gactacaagt tcttcgtgct
     5821 ctccgggaag gtgcgcctgc tggaggccga ctactcccgc ttcacgcggc acatgcaggc
     5881 gttcttcctt cccgacggca ggcggctggc ggtagatagg ggccgcgccg tgccggagct
     5941 gttgccgccg ttgccaacgc agttggcgga gatggtcgat ctggcggaga agctgggggc
     6001 gggtcacgag tacatcaggg tcgacctcta tgacgtcggg ggccgcacct atttcggaga
     6061 actgacgttc tacccgtccg gcgcgctcaa cctgtggcgt cccgcgcagt tcgagtacga
     6121 gatgggcgca gtgtggcggg agggcagacc gataggcgag gagtgggtgg ccggcgggta
     6181 gctcgccgtc aggcccagct ggcctcggac gccgccacga accctgggtt cgaaagcgcg
     6241 tcgccgacgt acctgagcgc ccgcccgccg ctcgcctcga ccatggaacc ccccgccgcc
     6301 tccaacaccg cctgcgccgc cgcggtgtcc caccacatcg ttgggccgag gcgcgcgtag
     6361 aagtgggcgg taccgtcggc gaccaagcac gtcttgatgg ccgagccgcg cgcgatggtc
     6421 tgcacctcgc cgtactcggc gcgcagacgt tcgatgaacg ccgccgtcga cgggttggag
     6481 tgcgagcggc tcaccatcac gcgcacgggc tcgcccgcgg tgggccggcg cacgtggatg
     6541 gggtgcgcgc cgccttccgc gtcgaagcgc caggcgctcg cgggcttgcc ggcgtgggag
     6601 gccagacccg tgtaggtcag gccggcgacc ggagcgtgga ccacgcccag caccggcacc
     6661 ccgtcctcga tcagggcgat gttcacggtg aact
//
LOCUS       DAOQYE010000011         6610 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_11998_length_6610_cov_3.212052, whole genome shotgun sequence.
ACCESSION   DAOQYE010000011 DAOQYE010000000
VERSION     DAOQYE010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 6610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6610
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11998_length_6610_cov_3.212052"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..787
                     /locus_tag="PKN52_00575"
     CDS             <1..787
                     /locus_tag="PKN52_00575"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HNQ98457.1"
                     /translation="ERHPVDKDELDLEHALAAAWARGATRVQVAGAIGNRLDQTLAAV
                     LIAARSARTGREVVLYGGRNEVHLVTRGASLTRGLAGGATISLLAMQEGCRVSIAGVR
                     YPLDASDLPWGSGLGVSNVATGGNVRLDVLAGTVALLIEHPEGDTRSAIWGAQKERIG
                     AALAAADPGLAEAIEGFVYDDIFSRPGLDLATRELLAVALLTSLGSTEELTTHLRGAL
                     RTGASERQVREAILHAAVFVGFPRALAAMRTLEDYLAGKPKRF"
     gene            complement(872..1537)
                     /locus_tag="PKN52_00580"
     CDS             complement(872..1537)
                     /locus_tag="PKN52_00580"
                     /inference="COORDINATES: protein motif:HMM:NF012455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HNQ98458.1"
                     /translation="MNIKPLVAEFIGTFTLIFAGVGAIAIGADLVGVALAHGIALAVM
                     ITAVAAVSGGHLNPAVTIGLWAAGKFPAGKILGYLIAQVAGAFVAAILIKLCLPAETA
                     VAGTGTPALAAGVGVGAAILLEAVLTFFLVFTVLGTAVDKRAPKLGGLLIGLSVTMGI
                     LVAGPITGAALNPARHLGPALLAGGSFLGQFWIYWVGPVLGGIVAGAVYKAFIEAPEG
                     KAN"
     gene            1715..2137
                     /locus_tag="PKN52_00585"
     CDS             1715..2137
                     /locus_tag="PKN52_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98459.1"
                     /translation="MTASDIQRRLAELGLPEGDYALHSSAALVLRGILDEAGDLDIVA
                     RGAAWRRALAMLDEGAVLDSGRQDLRVSVADDVEIYDGWMGDDADAVIGRSELVAGVR
                     CASLADIVAMKERLGRPKDVAHLGRIAAYLAGRRHPGG"
     gene            complement(2142..4598)
                     /locus_tag="PKN52_00590"
     CDS             complement(2142..4598)
                     /locus_tag="PKN52_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98460.1"
                     /translation="MRRSDRKTPRASLLLLAPFLWLLVSCGGPPNPPPNGGGVLTVNG
                     TVTLPSGHGLDLSKLTVSTPLGTYPVSATGEFTASVFGGAMTELGVETSDGSLLLLGV
                     TDGATGTISLTSTAEALLYYLVGGMWLPPAQQDKVRSLLRGVPEAADLAAELERQLSS
                     GGNGVAEPDAGLLAALEAAHASLLGESRLYGPTSGGVGFGEGGRAGSSRAVASSLLTT
                     SLGSVAANSPAHATVSVTVAPAAVDGSNIIIEPTARQAGVFVLHNPAGAGVVAQNHFR
                     RPAALLAYEEAWEDADRVEHPVDPPALVERVEVPATGQLEFMNALLDVVTGNSPWSPV
                     TSPALKLAGHEGASRTHYRLILVGPSATDATWPIMGDPLFTGFHDEWSDIAIDKSVEL
                     FLDDLLIPLMEVYGLGNMAKMDAAKLTKMRERVKIIYDKHLLGLGVYLKPGSASYANG
                     LRFVIQELVENRNFRLDMMNMVAEALEESDKNKAVVEAMEKRLSARASASAIAAAVQT
                     VLVSGDVAKIMYDLVGSPSVVDWTAVSAPALFALTPDQATVTRSNASARFTVVPKGRT
                     SGNYLYRWTTSGTYGELSDLLQDGTTLDTREPDVWYFHDSPLNIKDTDYDTIRVEVFE
                     VEAGATTIPAGASPIARMAGSVRGDDRVIDSRLEVNYGATPAGMFKDDLRFGCAEMLL
                     RFKAEKNAKSYLVSLRGVGGQGDERNANQDFRLRGPNQTVFIDPNAKIDGSIVEDGFT
                     SDYYGTCDWRSPYDPSRFALEPIGTSAHYDRANDEYVVYLFVNADYSGIVLNPVNLTE
                     RVDLWYDWVANATFEVKVNK"
     gene            4745..>6610
                     /locus_tag="PKN52_00595"
     CDS             4745..>6610
                     /locus_tag="PKN52_00595"
                     /inference="COORDINATES: protein motif:HMM:NF014731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HNQ98461.1"
                     /translation="MPLTLIALGVVFGDIGTSPLYAVREAFAKTHGLAVEPETVLGVL
                     SLVIWSLFLIVSVKYAVIAMRADRNGEGGILILTSLVTPLRGRPVGARRAFVLLGLFG
                     TALLVGDGMITPSITVLSAVEGLAIVAPRLEAFMLPIAIVILVVLFVAQRRGTDAVGK
                     VFGPIMLTWFVVIAALGVASVVREPGVLAALDPRHAATFFAHERWKAFLVLGSVFLAV
                     TGGEALYADIGHVGKRAARIGWFAVALPALLLNYAGQGALLLGSPDATGNPFFRLAPA
                     WALAPLVGLATLAAIIASQALISGVFSLTMQAVRLGYLPRLKVDHTSAKAFGQVYVGA
                     VNWLLLCACVALVLAFGSSSDLAAAYGVAVTATMLITSILLAVVAREVWRWSVPAVAA
                     SMGLFLLIDTAFLGANLAKVLHGGWFPLVVGALVFTVMTTWRRGRQELRARLGSSELA
                     IDRFLGSIRAAPPRRVPGTAVVMHGVKGVVPPVLLANLRYNHVLHQTVVMLAVETAEE
                     PRVLRAERTTVKAYGDGFFQVVLRFGFMERANVPAALADLVHPYLNLDPDVTTYFLGR
                     ELVVPAERRRPLPPQAGRKTGSPLPLWRQRLFALLARNAVNAAYYYSLPSEHVIEV"
BASE COUNT          980 a         2302 c         2283 g         1045 t
ORIGIN      
        1 cgagcgccac ccggtcgaca aggacgaact ggacctcgaa cacgcgctcg ccgcggcctg
       61 ggcgcgcggc gccacgcgcg tgcaggtcgc cggcgcgatc ggcaaccggc tcgatcagac
      121 gctggcggcc gtgctcatcg cggcccgctc cgccaggacc ggacgcgagg tcgtcctgta
      181 cggcgggcgg aacgaggttc acctcgtgac gcgaggtgcg agcctgacgc ggggtctggc
      241 gggcggcgcg acgatcagcc tcctggccat gcaagaaggg tgccgcgtca gtatcgccgg
      301 ggtccgctac ccgctggacg cgtccgacct gccgtggggg tcgggactgg gcgtctccaa
      361 cgtggcgacc ggcgggaacg tgcgcctaga cgtactggcc gggaccgtcg cgctgctgat
      421 cgagcatcct gagggcgaca cgcgttccgc catctggggc gcgcagaagg agcgcatcgg
      481 cgcggcgctc gccgcggccg accccggcct cgccgaggcc atcgaaggct tcgtctacga
      541 cgacatcttc tcccggcccg gcctcgacct cgccacgcgc gaactgctcg cggtggcgtt
      601 gctgacgtcg ctcgggtcca cggaggaact gaccacgcac ctgcgggggg ccttgcgtac
      661 cggggcttcc gagcggcagg tgcgcgaggc catcctccac gcggcagtgt tcgtcggctt
      721 cccgcgcgcg ctcgcggcca tgcgaacgct ggaggactac ctggccggca agccgaaacg
      781 gttctgaggg gccggaccag cacccctcag gcaaccctgc tcgttcaagc gccgcctcga
      841 ggcgttcgcg gcgccgcccc agcgagcgag gtcagttggc cttgccctcg ggcgcctcga
      901 tgaacgcctt gtagacggcg cccgcgacga tgccgccgag caccggaccc acccagtaga
      961 tccagaactg gccaaggaac gagccaccgg ccagcaacgc cggaccgagg tggcgagccg
     1021 ggttgagggc cgcgcccgtg atcggaccgg ccaccaggat gcccatggtg acggagaggc
     1081 cgatgagcag accgccgagc ttcggcgcgc gcttgtcgac ggcggtgccg agcaccgtga
     1141 agacgaggaa gaaggtgagg acggcctcga gcaggatggc ggcgccaacg ccgacgccag
     1201 ccgcgagcgc gggcgtaccc gtccccgcga cggccgtctc cgccggcagg cacagcttga
     1261 tgaggatggc ggccacgaaa gcgccggcca cctgcgcgat gaggtagccg aggatcttgc
     1321 cggccgggaa cttgcctgcc gcccagagac cgatggtgac ggccgggttg aggtgcccgc
     1381 cgctcacggc cgcgacggcg gtgatcatga ccgccagcgc gatgccgtgc gccagcgcca
     1441 cgccgacgag gtcggcaccg atggcgatgg cgccgacgcc ggcgaagatg agggtgaagg
     1501 tgcctatgaa ctcggcgacc aagggcttga tgttcatgca ggatcctttc tcatttagtg
     1561 ctcatcgaag atagtaacca ctggcgcggc ccttgtacac gccccgccgc ggggaccccg
     1621 ccccggccgg acgcaggccg gccatgttcg agccggaacg gctaccgcgg ggaccccacc
     1681 ccggccgggc acaagccgaa cgtatactcg ccgcgtgaca gcctccgaca tccagcgcag
     1741 gctcgccgaa ctcgggctgc cggagggcga ctacgcgctg cactccagcg cggcgctcgt
     1801 gctgcgcggc atcctggacg aggccggtga cctagacatc gtcgcgcggg gcgccgcgtg
     1861 gcggcgcgcc ctcgccatgc tcgacgaggg cgccgtcctg gacagcggcc gtcaggacct
     1921 ccgcgtgagt gtcgcggacg acgtcgagat ctacgacggc tggatgggcg acgacgccga
     1981 cgccgtgatc gggcgctccg agctcgtcgc cggcgtgcgc tgcgcgagcc ttgccgacat
     2041 cgtcgccatg aaggaacggc tcggacggcc caaggatgtg gcgcacctgg ggcgcatagc
     2101 ggcctacctg gccgggcggc gccaccccgg cggctagacg ctcacttgtt gaccttcacc
     2161 tcgaacgtgg cgttggcgac ccagtcgtac cagaggtcca cccgctccgt gaggttcacc
     2221 gggttaagga cgatccccga gtagtcggcg ttgacgaaga ggtacacgac gtactcgtcg
     2281 ttggcccggt cgtagtgggc cgaggtgccg ataggctcaa gcgcgaaccg cgacgggtcg
     2341 taaggggagc gccagtcgca ggtgccgtag tagtcggacg tgaaaccgtc ctcgacgatg
     2401 ctgccgtcga tcttcgcgtt cggatcgatg aacacggtct ggttgggacc gcgcaggcgg
     2461 aagtcctggt tggcgttgcg ctcgtctccc tgaccgccga cgcctctaag cgagaccaag
     2521 tagctcttgg cgttcttctc ggccttgaag cgcaggagca tctcggcgca gccgaagcgc
     2581 aggtcatcct tgaacatccc ggcaggcgtg gcgccgtagt tcacctcgag gcgcgagtcg
     2641 atgacgcggt cgtcgccccg tacggagccc gccatgcgcg cgatcgggct ggcgccggcc
     2701 gggatggtgg tcgccccggc ctcgacctcg aagacctcca ccctgatcgt gtcgtagtcg
     2761 gtgtccttga tgttcagggg cgagtcatgg aagtaccaca cgtcgggttc gcgggtgtcg
     2821 agcgtcgtgc cgtcctggag caggtcgctg agctcgccgt acgtcccgct cgtcgtccag
     2881 cggtacaggt agttccctga cgtcctgccc tttggcacca cggtgaagcg ggccgaggcg
     2941 ttgctgcgcg tgacggtcgc ctggtcgggc gttagagcga agagggccgg cgcagacacc
     3001 gccgtccagt cgacgacgct gggcgagccg acgaggtcgt acatgatctt cgcgacgtcg
     3061 ccgctcacga ggacggtctg cacggcggcc gcgatggcgc tggcgctggc gcgcgcgctg
     3121 agccgcttct ccatggcctc cacgaccgcc ttgttcttgt cgctctcctc cagcgcctcc
     3181 gcgaccatgt tcatcatgtc gaggcggaag ttgcggttct cgacgagttc ctggatcacg
     3241 aacctcagac cgttggcgta cgaggccgag ccgggcttca agtacacccc caggccgagc
     3301 aggtgtttgt cgtagatgat cttcacgcgc tcgcgcatct tggtcagctt ggcggcgtcc
     3361 atcttcgcca tgttcccgag gccgtagacc tccatcagcg ggatgaggag gtcgtccagg
     3421 aagagctcga cggacttgtc gatggcgatg tcgctccact cgtcgtggaa gcccgtgaag
     3481 agcgggtcgc ccatgatcgg ccaggtggcg tcggtggcgc tcggccccac caggatcagg
     3541 cggtagtgcg tgcggctcgc tccctcgtgg ccggccagct tgagggccgg gctcgtgacg
     3601 ggcgaccagg gcgagttgcc tgtcaccacg tcgaggaggg cgttcatgaa ctcgagctgg
     3661 cccgtggcgg gtacctcgac gcgctctacc agcgcgggcg ggtcgacggg gtgctcgacc
     3721 cggtcagcat cctcccacgc ttcctcgtag gcgagcaggg cggctgggcg acggaagtgg
     3781 ttctgcgcca cgacgccggc gccggccggg ttgtgcagta cgaagacgcc ggcctgccgc
     3841 gccgtgggct cgatgatgat gttgctgccg tcgacggccg ccggggcgac ggtcacgctc
     3901 acagtggcgt gagccgggga attggcagcg acggaaccga ggctcgtggt caagagggag
     3961 gacgctacgg cccggctcga gccggccctt ccgccctcgc cgaatccgac acccccggaa
     4021 gtcggtccgt atagccgcga ctcgccaagc agcgaagcgt gcgccgcctc gagcgcggcc
     4081 agcaggccgg cgtcgggctc cgcgacaccg ttcccacccg acgacaactg ccgctccagc
     4141 tccgccgcca ggtcggcggc ctccggcaca ccgcgcagca gcgaacggac cttgtcctgc
     4201 tgggccggcg gcagccacat gccgcctacg aggtagtaca ggagcgcctc ggccgtgctc
     4261 gtcagcgaga tggtccccgt ggccccgtcc gtgaccccga ggagcagcag tgagccgtca
     4321 gacgtttcga cgccgagttc cgtcatcgcg cccccgaaga cggaggccgt gaactcgccc
     4381 gtggccgaga cgggataggt accgagcggc gtggagaccg tgagcttcga gagatcgagg
     4441 ccgtgaccgc tcggcagcgt gacggtgccg ttgacggtga gcacaccgcc cccgttgggc
     4501 ggcgggttgg gcgggccacc gcaggagacc agcaaccaca ggaacggcgc caagagcagc
     4561 agcgaagcgc gtggggtctt ccggtccgat cttctcatga tcagaaagta gtcgacgaag
     4621 acttaaatgc ggcttaaccg tcggtgaccc gcgtcacgcc cccgtctcgg cgaggactta
     4681 cagtgtgcgc cgctcgcagc aagcgagcac tatgacgacc cctaccccca cagccaagcg
     4741 acacttgccg ctcaccctga tcgcgctcgg cgtggtcttc ggcgacatcg gtaccagccc
     4801 cctgtacgcc gtgcgggagg cgttcgccaa gacgcatggg ctggcggtcg agccggaaac
     4861 ggtgctaggg gtgctctcgc tcgtcatctg gtcgctcttc ctcatcgtct ccgtgaagta
     4921 cgccgtcata gccatgcggg ctgaccgcaa cggggaggga gggatcctca tcctcacgtc
     4981 gctcgtcacg ccgctgcgag gacgcccggt cggggcacgg cgagcgttcg tgttgctcgg
     5041 gctcttcgga acggccctgc tggtcggcga cggcatgatc acgccgtcta tcacggtgtt
     5101 gagcgccgta gaaggcctgg ccatcgtggc cccgcgccta gaggcgttca tgctacccat
     5161 cgccatcgtc atactggtgg tcttgttcgt cgcgcagcgc cgcggcacgg atgccgtcgg
     5221 taaagtgttc ggcccgatca tgctcacgtg gttcgtggtc atcgcggcgc tcggcgtagc
     5281 gagcgtggtc agggagcccg gcgtcctcgc ggcgctcgac ccccggcacg ccgccacctt
     5341 cttcgctcac gagcgttgga aggccttcct cgtcctcggc tccgtgttcc tagccgtcac
     5401 gggcggagag gcgctctacg ccgacatcgg tcacgtgggc aaacgcgccg cgcgcatcgg
     5461 atggttcgcc gtcgcgttac cggctctgct actcaactac gccggccagg gcgcgctcct
     5521 gcttggctcc cccgacgcca ccggcaaccc cttcttccgg ctcgcgccag cctgggcgct
     5581 cgccccgctg gtcggcctcg ccacgctggc cgccataatc gcctcccagg ccctgatctc
     5641 cggggtcttc tcgctgacca tgcaggccgt ccgcctcggc tacctgccgc ggctcaaggt
     5701 cgaccacacg tccgccaagg ccttcgggca ggtctacgtc ggcgccgtca actggctcct
     5761 actgtgcgca tgcgtggcgc tcgtcctggc gttcggatcc tcgagcgacc tggcggcggc
     5821 ctacggcgtc gccgtcactg cgaccatgct catcacctcc atcctcctgg ccgtcgtcgc
     5881 gcgcgaagtc tggcgctgga gcgtcccggc cgtcgccgct tccatgggcc tgttcctcct
     5941 gatcgacaca gccttcctag gcgccaacct cgccaaggtc ctgcacggcg gctggttccc
     6001 gctcgtcgtg ggggcgctcg tcttcacggt catgacgacg tggcggcgcg ggcgccagga
     6061 actgcgcgcg cggctcggct ccagcgagct ggccatcgac cgcttcctag gcagcatccg
     6121 ggcggcacca ccgcggcgcg tgcccgggac ggccgtggtc atgcatggtg tcaagggcgt
     6181 cgtccctccc gtgctccttg ccaacctccg ctacaaccac gttctgcacc agacggttgt
     6241 gatgctcgcg gtggagacgg ccgaggagcc gcgggtgctg cgggccgagc ggactaccgt
     6301 gaaggcgtac ggcgacggct tcttccaggt ggtgctccgg ttcgggttca tggagcgcgc
     6361 taacgtcccg gcggcgctcg ccgacctcgt acacccctac ctcaacctcg acccggacgt
     6421 gaccacgtac ttcctcggtc gcgaactcgt agtgccggcc gaacgccgcc gtccgctgcc
     6481 gccgcaagcc gggcggaaga ccggaagccc gctgccgctc tggcgccaac ggctgttcgc
     6541 gctgctggcg aggaacgccg tcaacgcggc ctactactac tcgcttcctt cggagcacgt
     6601 catcgaggtg
//
LOCUS       DAOQYE010000012         6414 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_12474_length_6414_cov_3.366095, whole genome shotgun sequence.
ACCESSION   DAOQYE010000012 DAOQYE010000000
VERSION     DAOQYE010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 6414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6414
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12474_length_6414_cov_3.366095"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(96..809)
                     /locus_tag="PKN52_00600"
     CDS             complement(96..809)
                     /locus_tag="PKN52_00600"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HNQ98462.1"
                     /translation="MGARRVIGVMGGLGPAATVDFYAKLVAATPAARDQDHLHVLIDA
                     DPTTPNRNEAIAGTGPSPEPQLIGMATRLAAAGAEALFMVCNAAHAYAPAVRAAVGVP
                     LVSIIEETAAAAMAAAPGLRRVGVLAAAGAQDARLYATEFARHGVATLEPRGELRERF
                     MALLYRIKGGDAGPEVRSGMRAIACELVAQGAEVVVAGCTEVPLVLTQAELGPTPLVS
                     STDALVAAAVAIGTGARPL"
     gene            complement(809..1807)
                     /locus_tag="PKN52_00605"
     CDS             complement(809..1807)
                     /locus_tag="PKN52_00605"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF003031.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HNQ98463.1"
                     /translation="MKLTDFPRRRYTAGATPLEPLPRLSAHLGGPNLWIKRDDLLGLA
                     GGGNKTRKLEFLVADAIEKGADTLVTTGAVQSNHCRLTLAAAVKEGLACRLVLEERVA
                     NSYSAEASGNNFLFGLLGVEAVTVVPAGTDLMARMTEVAADLAAQGRSAYVIPGGGSN
                     TLGALGYVSCAQELMQQAFETGLRIDHLVTASGSGGTHAGVVAGLTGTSAGVPVTGIS
                     VRAAKAPQEEKILGLARATAELAGSRFLPTSDDVVVLDDYVGEGYSLPTDGMIEAVKL
                     FARLDGVLLDPVYTGKAAAGLIDLVRSGAFGRHDNVVFLHTGGSPALYAYQDVLAG"
     gene            1897..2904
                     /gene="deoC"
                     /locus_tag="PKN52_00610"
     CDS             1897..2904
                     /gene="deoC"
                     /locus_tag="PKN52_00610"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HNQ98464.1"
                     /translation="MTISRDVEPRLERAVGHDSPLPRNEGTALELDWVESVRVNLSAV
                     ERRAATLTTRRSVKKDWQAAWLLKAVTLIDLTTLAGDDTPGRVRRLCAKAMHPVRQDL
                     LDALGVGDLRITTGAVCVYHDMVPAAVAALGNSGVPVAAVSTGFPAGLSPLETRIEEI
                     RRSVAAGAAEIDIVISRRHALTHDWEALYEELRLFRQACGPAHMKAILATGELGTLRN
                     VYRASLVAMMAGSDFIKTSTGKEPVNATLPVSLVMARAIREYSERTGVAVGFKPAGGL
                     RTAKDALAWLILIKEELGRRWLEPDLFRIGASSLLADIERQLEHHVTGRYSATYRHPL
                     G"
     gene            2980..5397
                     /locus_tag="PKN52_00615"
     CDS             2980..5397
                     /locus_tag="PKN52_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNQ98465.1"
                     /translation="MSVKEIFETMDYGPAPEAADVANAWLERHGRRLGHFIGGRFVKP
                     STGETFATHHPGDGKELAQVAQGDERDVAKAVKAARAALPAWRDLGGPGRARYLYALA
                     RHVQKHARLLAVLESLDNGKPIRETRDLDVPLVARHFYHHAGWAQLLDREFPDDEGVG
                     VCAQIIPWNFPLLMLAWKVAPALAAGNTVVLKPAEFTPLTALLFAELANEVGLPAGVF
                     NVVTGDGRTGAALVDADGIDKVAFTGSTEVGRIIRRATAGRGIKLSLELGGKSPFIVF
                     EDADLDSAVEGVVDAIWFNQGQVCCAGSRLLVQESVAKRIEAKLIARMETLREGPSLD
                     KGIDIGAIIAPVQLSKIERLVDQGRAEGARCYQPSWSAPKDGWYYPPTLFTEVSPAAV
                     IAQEEIFGPVIVSLTFRTPAEAVELANDTRYGLAASVWTENVNLALDVAPKLKAGVVW
                     VNCTNQFDAAAGFGGYRESGFGREGGREGMFEYLKPKYEARLARYASADLVPPASASD
                     LEAGLGGGLEAAAAFAPIDRTPKLYIGGKQKRPDGNYSYPVVSAAGARVGDAPLGNRK
                     DIRDAVEAAVKSTAWTQATAHARAQVLYYVAENLAVRAPEFEERLRSLVGGTRKSAGR
                     EVEKSVERLFSAAAYADKYDGRVHPTPMRNVTLAMNEPYDVLAIVCPDEAPLLAFVSL
                     VAPALAMGNRVVVVPSARWPLLATDLYQVFDTSDVPGGAVNIVTGVRDELARVLAQHD
                     AVEAMWYVGSAEGSAMVERESAGNLKATWVNSGKARDWYDDAQAAGPELLRRAVRVKN
                     IWVPYGE"
     gene            5611..6030
                     /locus_tag="PKN52_00620"
     CDS             5611..6030
                     /locus_tag="PKN52_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HNQ98466.1"
                     /translation="MSKFAKLLLAAVVAFGFGAAFAQDQEPTVEFTLHGGIGTAGFQF
                     TGVGGDIDGVVNPDLNVKVGDVVKVTIISDGMEHDFVIDDLGVKSESVMGAELSVSVV
                     FTVTEAGTFTYYCSTPGHALPDLGIGMFGNFIVAAAD"
     gene            6178..>6414
                     /locus_tag="PKN52_00625"
     CDS             6178..>6414
                     /locus_tag="PKN52_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98467.1"
                     /translation="MTEVRGAGLEKVERRIVSPQGASVEVASARGTVTALNLCANNYL
                     GLANHPALVEAGREALAKRGFGMASVRFICGTQDL"
BASE COUNT          957 a         2262 c         2250 g          945 t
ORIGIN      
        1 tagcacggtg tccaggcggt cgaggctcat gatccgttga gggaaccggg tcacccggcc
       61 ggggtggccc ggtccggctg gggtggcccg gccggtcaga gcggccgcgc gccggtccct
      121 atggcgacgg cggcggccac gagggcgtcc gtggaggaga cgagcggggt cggaccgagc
      181 tcggcctggg tcagcacgag cggtacctcg gtgcagccgg ccaccacgac ctcagcgccc
      241 tgagcgacga gttcgcaggc gatggcgcgc atgcccgagc gcacctcggg gccggcgtcg
      301 ccgcccttga tgcgatacag gagcgccatg aagcgctcgc ggagctcgcc ccgaggctcg
      361 agcgtggcca cgccgtggcg ggcgaactcg gtggcgtaga gtcgcgcgtc ctgtgcgccg
      421 gcggccgcga gcacgcccac acgccggagc cccggtgcgg cggccatggc cgccgccgcg
      481 gtctcctcga tgatgctcac gagcggcact cccaccgccg cgcggacggc cggcgcgtac
      541 gcgtgcgcgg cgttgcagac catgaagagc gcctcggccc cggcggccgc gaggcgcgtc
      601 gccatgccga tcaactgcgg ctccgggctc ggacccgtgc cggctatggc ctcgttgcgg
      661 ttcggcgtgg tcgggtcggc gtcgatgagc acgtggaggt gatcctggtc gcgcgccgcg
      721 ggagtggcgg cgaccagctt ggcgtagaag tccaccgtgg cggccggccc gagcccgccc
      781 atgacgccga tgacacgccg cgcgcccatc aaccggccag gacgtcctgg taggcgtaga
      841 gcgccgggct gccgcccgtg tgcaggaaga cgacgttgtc gtggcgcccg aaggcgccgc
      901 tccgcaccag gtcgatgagt ccggccgcag ccttgcccgt gtacacgggg tcgaggagta
      961 cgccatcaag ccgcgcgaag agcttgacgg cctcgatcat gccgtcggta ggcagcgagt
     1021 agccttcgcc aacgtagtcg tcgagtacga cgacgtcgtc cgacgtgggc aggaagcgcg
     1081 agccggccag ctccgccgtc gcccgcgcga ggccgaggat cttctcctcc tgcggtgcct
     1141 tggccgcgcg cacgctgatg cccgtgaccg gcacgccggc gctcgttccc gtaaggccgg
     1201 ccacgacgcc ggcgtgcgtg ccgccgctgc cgctggccgt cacgaggtgg tcgatgcgca
     1261 agcccgtctc gaacgcctgc tgcatgagct cctgcgcaca gctcacgtag ccgagcgcgc
     1321 cgagggtgtt cgagccgccg ccggggatga cgtaggccga ccgcccctgc gccgccaggt
     1381 cagcggccac ctcggtcatc ctcgccatga ggtcggtgcc ggcaggcacg acggtgacgg
     1441 cctcgacgcc gaggaggccg aagaggaagt tgttgccgct ggcctcggcg gagtagctgt
     1501 tcgcgacgcg ctcctcgagg accaggcggc aggccagtcc ctccttcacg gccgcggcga
     1561 gcgtcaggcg gcagtggttc gactgcacgg cgccggtggt gacgagcgtg tcggcgccct
     1621 tctcgatggc gtcggcgacg aggaactcga gcttgcgcgt cttgtttccg ccgccagcga
     1681 gacccaggag gtcgtcgcgc ttgatccaga ggttcggccc gcccaggtgg gcgctcagtc
     1741 gcgggagcgg ttcgaggggc gtggcgcccg ccgtgtagcg gcggcgcggg aagtcggtga
     1801 gcttcatggt tcgcctccat ggggcggctc gcggatggca ccagcgcaaa gtccgaccca
     1861 gacgccagtg tatgctccgc ccataggaga gaaggcatga ccatctcgcg tgacgtagaa
     1921 ccaaggctcg agcgggccgt aggtcacgac tcgcccctgc cgcgcaacga gggcacggcc
     1981 ctcgagctcg actgggtcga gagcgtgcgc gtcaacctca gcgccgtcga gcgccgcgcc
     2041 gcgaccctca ccacgcgccg cagcgtcaag aaggactggc aggccgcgtg gctcctcaag
     2101 gccgtcacgc tcatcgacct gacgacgctg gcgggcgacg acactccggg tcgcgtgcgg
     2161 cgcctctgcg ccaaggccat gcaccccgtg cgccaggacc tgctggacgc gctcggtgtc
     2221 ggcgacttgc gcatcaccac gggtgccgtg tgcgtctatc acgacatggt gccggccgcc
     2281 gtcgccgcgc tcggcaactc cggcgtgccc gtcgcggccg tctccacggg cttcccggcc
     2341 ggtctctcac ccctcgagac gcgcatcgag gagatccgcc gctccgtcgc ggccggcgcc
     2401 gccgagatcg acatcgtcat ctcgcgccgc cacgccctca cgcacgactg ggaggcgctc
     2461 tacgaggagc tgcgactgtt caggcaggcg tgcggcccgg cgcacatgaa ggccatcctc
     2521 gccactggcg agctcggcac cctccgcaac gtctaccgcg cgagcctcgt agccatgatg
     2581 gccggctctg acttcatcaa gacgtcgacc ggcaaggaac ccgtcaacgc caccctaccc
     2641 gtcagcctcg tcatggcgcg cgccatccgc gagtactcgg agcggacggg cgtcgcagtc
     2701 ggtttcaagc cggccggcgg gctgcgcacc gccaaggacg ccctagcgtg gctcatcctc
     2761 attaaggagg agttggggcg ccgctggctc gagccggacc tcttcaggat cggcgccagc
     2821 tcgctgctgg ccgacatcga gcgccagcta gagcaccatg tgaccggccg ctactccgcc
     2881 acgtaccgcc acccgctcgg ctgatggcct gggccgcccg tccgcgcgga tccggccgtg
     2941 cgacaggcac gcctgccgac ttaaaggagt ctttgagaga tgagcgtgaa ggagatcttc
     3001 gagacgatgg actacggtcc ggctcccgag gcggcggacg tcgccaacgc ctggctggag
     3061 cgtcacggta ggcgcctcgg tcacttcatc ggcgggcgct tcgtcaagcc gtccacgggc
     3121 gagacgttcg ccacgcatca tccgggcgac ggcaaggaac tggcccaggt cgcgcagggc
     3181 gacgagcgcg acgtcgcgaa ggccgtcaag gcggccaggg cggcgctgcc ggcctggcgc
     3241 gacctcggcg gccccggccg ggcccgctac ctatacgctc tcgcccgcca cgtgcagaag
     3301 catgcgcggc tcctcgcggt cctcgagtcg ctcgacaacg gcaagcccat ccgcgagacc
     3361 cgcgacctcg acgtgcccct ggtggcgcgc cacttctacc accacgccgg ctgggcgcag
     3421 ctcctcgatc gggagttccc tgatgatgag ggcgtcggag tctgcgcgca gatcatcccc
     3481 tggaacttcc cgctcctcat gctcgcttgg aaggtggccc ccgccctcgc ggccggcaac
     3541 accgtcgtgt tgaagccggc cgagttcacg cctctcacgg cgctgctctt cgcggagctc
     3601 gcgaacgagg tcggcctgcc tgccggcgtc ttcaacgtcg tgacgggcga tgggcgcacg
     3661 ggcgccgcgc tcgtcgacgc cgacggtatc gacaaggtcg cgttcacggg ctcgaccgaa
     3721 gtggggcgca tcatccgccg cgcgacggcc gggcgcggca tcaagctatc gctggaactc
     3781 ggcggcaagt cgccgttcat cgtcttcgag gacgccgacc tcgacagcgc cgtcgagggc
     3841 gtcgtggacg ccatctggtt caaccagggc caggtctgct gtgctggcag ccgcctcctc
     3901 gtccaggaga gcgtcgccaa gcgcatcgag gccaagctca tcgcccgcat ggagacgttg
     3961 cgcgaggggc cgtcgctcga caagggtatc gacatcggcg cgatcatcgc gcccgtccag
     4021 ctctccaaga tcgagcgcct cgtcgaccag ggccgagccg aaggcgcgcg gtgctaccag
     4081 ccgagctgga gcgcccccaa ggacggctgg tactacccgc cgaccctctt caccgaggtc
     4141 tcgccggccg ccgtcatcgc ccaggaggag atcttcgggc cggtgatcgt cagcctcacg
     4201 ttccgcaccc cggccgaggc cgtcgagttg gccaacgaca cgcgctacgg cctcgccgcg
     4261 agcgtgtgga cggagaacgt caacctggcg cttgacgtgg cgccaaagct caaggcgggc
     4321 gtcgtgtggg tcaactgcac caaccagttc gacgcggccg ccggcttcgg cggctaccgc
     4381 gagagcggct tcgggcgcga gggcggtagg gaggggatgt tcgagtacct caagcccaag
     4441 tacgaggcta ggttggctcg ctacgcgagc gcggacctcg tgccgcccgc gtcggcctcg
     4501 gacctcgagg ccggactagg tggcgggttg gaggccgctg cggctttcgc cccaatcgac
     4561 cgcacgccga agctctacat cggcggcaag cagaagcgcc ccgacggcaa ctactcctac
     4621 ccggtcgtga gcgcggccgg tgcgcgcgtc ggcgacgccc ccctgggcaa ccgcaaggac
     4681 atcagggacg ccgtcgaggc cgcggtcaag tccaccgcct ggacccaggc gacggcccac
     4741 gctcgcgccc aggtcctcta ctacgtggcc gagaacctgg ccgtgcgcgc tcccgagttc
     4801 gaggagcgcc tgcgcagcct ggtcggcggc acgcgcaagt ccgccggtcg cgaggtggag
     4861 aagagcgtcg agcgcctgtt ctccgccgcc gcctacgccg acaagtacga cggtcgcgtt
     4921 cacccgaccc ccatgcgcaa cgtgacgctg gccatgaacg agccgtacga cgtcctcgcg
     4981 atcgtgtgcc cggacgaggc tccgctcctc gcgttcgtgt cgctcgtagc gcccgcgctg
     5041 gccatgggca accgcgtggt cgtcgtgccg agcgcgcgct ggccgctact cgccaccgac
     5101 ctctatcagg tgttcgacac gtccgacgtg cccggaggcg ccgtgaacat cgtgacgggc
     5161 gtgcgcgacg agctggccag ggtcctcgcc caacacgacg ccgtcgaggc catgtggtac
     5221 gtgggctcgg ccgagggcag cgccatggtg gagcgtgaga gcgccggcaa cctcaaggcc
     5281 acgtgggtga actccggcaa ggcgcgcgac tggtacgacg acgcccaggc cgccggcccg
     5341 gagctccttc ggcgggcggt gcgcgtcaag aacatctggg tgccgtacgg cgagtgaggt
     5401 cgtcggcccg ggctcgagcg ttcgctttcg gttaaggaac tgcgtagccg ccgctaagcc
     5461 atggccggcc gactctggtt aacatcctgt cgtttgccgc acgccggtcg cagcagctac
     5521 cggctcgacg gtgacaggtc gtgagtggct tcgggtcgct cggccgatag aggaccgagg
     5581 gccggcggga agccggagag gcagtgtcag atgtccaagt tcgccaagct tctattggcc
     5641 gcggtcgtgg cgttcggttt cggggcggcg ttcgcccagg accaggagcc gaccgtcgag
     5701 ttcaccctgc acggcgggat cggcacggcc ggtttccagt tcaccggagt cggtggcgat
     5761 atcgatggcg ttgtgaaccc cgacctaaac gtcaaggtgg gcgacgtcgt caaggtcacc
     5821 atcatcagcg acggcatgga gcacgacttc gtcatcgatg atcttggtgt caagtccgag
     5881 tcggtcatgg gcgccgaact cagcgtcagc gtcgtgttca ccgtcaccga ggccggcacg
     5941 ttcacctact actgcagcac gccaggtcat gcgctgcctg acctcggcat cggcatgttc
     6001 ggcaacttca tcgtggccgc ggccgactaa gcacgaagac tcgttaggag tcgcctgtgc
     6061 gggcgcgctc gggaatgccc gggcgcgccc gtcgcatacc gggcgcccgt cgcatgttgg
     6121 taacctgagc cgactatgag ccgttggcag cgattcaccg atcacttatc agcgtcgttg
     6181 accgaagtgc gtggcgcggg cctcgagaag gtcgagcgcc gcatcgtgtc gccgcaggga
     6241 gcctcggtcg aggtggcgag cgcgcgcggc accgttaccg ccctcaacct gtgcgcgaac
     6301 aactacctcg gtctcgccaa ccaccccgcc ctcgtcgagg ccggcaggga ggcgctcgcc
     6361 aagcgcggct tcggcatggc gtccgtgcgc ttcatctgcg gcacccagga cctg
//
LOCUS       DAOQYE010000013         6358 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_12598_length_6358_cov_3.370776, whole genome shotgun sequence.
ACCESSION   DAOQYE010000013 DAOQYE010000000
VERSION     DAOQYE010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 6358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6358
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12598_length_6358_cov_3.370776"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="PKN52_00630"
     CDS             complement(<1..344)
                     /locus_tag="PKN52_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004668 - protein-arginine deiminase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HNQ98468.1"
                     /translation="MPTTPPPTPASLGFRMPPEWAPHAATWTSWPFDDRLWVGHLEGV
                     RREFADLVATIARFEPVVLNVRDGEAEKDARARIANAAAARHGDEAERVLGNVTYNLL
                     PLNDVWFRDNGPL"
     gene            complement(357..1166)
                     /locus_tag="PKN52_00635"
     CDS             complement(357..1166)
                     /locus_tag="PKN52_00635"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HNQ98469.1"
                     /translation="MSNPHKAIRIAAHRGWSAAFPENSLAAFAAAYSAGVDELEFDVR
                     HTMDGVPVIVHDARMARTSDSEAEVACLSIDEFLAASLREPGGSVTPGHTPPTLLQVL
                     DLFGGRIGMNIHVSDNDPPASTFSMLAQLPWANDNPDVYIAGRENVLERALQHAPRVH
                     RACLVNQNKPEEMLYAAKAFRCQRVQFFKESVTEALVASAKSQGIASNLFFSDELEEA
                     LRLYDLGLETLLTNDAGPLVRARDAYRARKFGGSRREPITPRLPPKSSSPA"
     gene            complement(1228..2730)
                     /locus_tag="PKN52_00640"
     CDS             complement(1228..2730)
                     /locus_tag="PKN52_00640"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNQ98470.1"
                     /translation="MPSRKLLLTLLFAQLALFALARDLHIAVAGIDANLEPGIATSNT
                     GMQIVPNLFETLIEMDMQDNSVLKPKLATSWHRVDDLTLELTLREGVTFHNGDPFGAE
                     DVKFSFERFMNPDYRGSLSRSLFATITAVDIVDDHTVRISTAQPDPILEYRLASLWGA
                     WIVPAKHYQAVGADAFGIEPVGTGFYRLSDRQPDRIELTAFAGYWGARPAAERVVYRI
                     IPEVSTRVAALIAGEVDIVAALPADQYASVERNADLRVERTLINNIHMLVYNTWDSPI
                     TDPKLRQALNLGIDRQLIVDALWHGDAEVPRSHQFAAYGPLFNAERQVQPYDPDRARQ
                     LIAESGYDGSPVYYLARHTGYVNELPVAEIIVQMWQDLGIDAHVSVVDPSQLDLNTAS
                     VGTWSNTMRFPDPAGGLWLLWNETGTQQIRGWTPRNGFNEVGHELEIAQGIQERFELS
                     QRLLDLWEEEAPGTVLWFPSEAFGVSNTVVWKPNALQTIDLRAELLSFID"
     gene            complement(2816..3748)
                     /locus_tag="PKN52_00645"
     CDS             complement(2816..3748)
                     /locus_tag="PKN52_00645"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ98471.1"
                     /translation="MVTTAAETAAREPTTRHDPTRRSRRLPRLFPTSMPWLIRAQVIN
                     LALLAGLALLGPSVIGHDPARQDLRARLTAPVWIGNHANHPLGTDHIGRDVLARTVYG
                     LRTSLIVAAIGTVVSLVIGTALGALAALGGRAADAVVMFLVETQMSVPYTLVVLVLMA
                     SLGTDIRVFLLVVGLAGWEGVARLVRGQLLAAKRQDFVEGARALGLGQLRIAFRHLLP
                     TTAPILMVLASGSFTGVIFLESSLSFLGVGIQPPATSLGLLIGVGRDHLMGAWWIALA
                     PCALLVSLTLSVALLGDWLRDRLDPRARRKLTRR"
     gene            complement(3751..4671)
                     /locus_tag="PKN52_00650"
     CDS             complement(3751..4671)
                     /locus_tag="PKN52_00650"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ98472.1"
                     /translation="MLKYLLQRLALAAATLWFIATLVFCSARLTGDPTHWLLPEDAPP
                     QARQELRESLGLDRPLLAQYGTYLTGLIRGQVATSYYEPRPVREILMERLPNTLRLAG
                     LALAGTWLFGLLLGGLAAVNHNGVLDRTIVAVASLVSAVPSFVVAIGMILMFSVVLRI
                     LPTSGTGTWRNLVMPVATLTLLGAAGLVRLVRGSLLETLDQDFVRTARAKGLRRWGTV
                     GKHAMRNAAIPLLTVFGTQLGALVAGTVVVETVFAWPGLGRAIVAAVNTRDFPLLQTS
                     ILLVSGTVIACNLLVDLLYPMVDPRVRLGG"
     gene            complement(4764..5579)
                     /locus_tag="PKN52_00655"
     CDS             complement(4764..5579)
                     /locus_tag="PKN52_00655"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HNQ98473.1"
                     /translation="MRTVEALRRTQHAYPRLSRSERAVADLIRTRPTEVALLSLQALA
                     QQCGTSEASVLRFCRSIGYSGYQDFKSGLVVDLLKHQPPTASTDAHGDTGGADIWTNV
                     QTHATRGLEQTIAMLDRHLVEELASRILASRRVAVLGVSGSAGVATVFAFGLLACGIE
                     AIHPVDRVEIERLCSLLSPEVVVIGISHSGNAPEVVTGLERAVQAGCFNVAVTHFELS
                     PAARAADRLILTCGQEPLLGSNACTPRALQLIALEIVLEHVAHGLKDVQDRSI"
     gene            complement(5710..6231)
                     /locus_tag="PKN52_00660"
     CDS             complement(5710..6231)
                     /locus_tag="PKN52_00660"
                     /inference="COORDINATES: protein motif:HMM:NF014999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF234 domain-containing protein"
                     /protein_id="HNQ98474.1"
                     /translation="MADRTLRLFHVFVSPYTSLLERSDPRQVYAQVVAPRLDTFMGLA
                     FEDLVAPTYERLRDPRVLPLVERWSRWEGRDRSGRSLEVDVVAPLVDKRLMTGAVKYS
                     RTPIGPSVFYDHLAALQRAADAGLKWAHRAMNDGPLIFMSASGFTERFADAANAYKAP
                     VLAWSLQDVYSAA"
BASE COUNT         1029 a         1939 c         2322 g         1068 t
ORIGIN      
        1 agggggccgt tgtcgcggaa ccagacgtcg ttcaggggta gcaggttgta ggtcacgttg
       61 ccgagcacgc gttccgcttc gtcgccgtgc cgggcggccg cggcattcgc gatgcgcgcg
      121 cgggcgtcct tctcggcctc gccgtcgcgc acgttaagaa cgacgggctc gaagcgcgcg
      181 atggtggcca cgaggtcagc gaactcgcgc cttacccctt cgaggtgccc gacccagagc
      241 cggtcgtcga acggccatga cgtccaggtg gcggcgtgcg gagcccactc agggggcatg
      301 cggaagccca gtgatgctgg ggtcggggga ggggtcgtgg gcatgcttgg gccagtttag
      361 gccggggacg aggacttggg cggcaatcgt ggggtgatcg gttcgcgtct acttccaccg
      421 aacttgcgcg cgcggtaagc gtcacgcgcc cgcacgagcg gcccggcatc gttcgtgagc
      481 agggtctcca acccaaggtc gtagagccgt agcgcttcct ccagctcgtc ggagaagaag
      541 aggttgctgg ctatgccctg gctcttggcg ctcgcgacaa gagcctccgt cacgctctcc
      601 ttgaagaact gcacacgctg gcaccggaac gctttcgctg cgtagagcat ctcttccggc
      661 ttgttctggt tgacgaggca ggcgcggtgc accctcggag cgtgttgcaa cgcgcgctcc
      721 agaacgttct cgcgcccggc gatgtagacg tccgggttgt cgttggccca aggcagttgc
      781 gccagcatgg agaaagtgga ggcgggaggg tcgttgtccg acacgtggat gttcatgcct
      841 atgcgcccgc cgaacaagtc gagtacctgc agcaatgtcg gtggcgtgtg gccaggcgtg
      901 accgagccgc cgggctcgcg cagcgacgcg gctaggaact cgtcgatgct caggcaggcg
      961 acctcagctt ccgagtcgct cgtgcgcgcc atgcgcgcgt cgtggacgat gacgggcaca
     1021 ccgtccatgg tgtggcgtac atcgaactcg agttcgtcga cccccgccga gtaggcggcg
     1081 gcgaacgctg cgaggctgtt ctcgggaaag gcggcgctcc agccgcggtg ggctgcgatc
     1141 cttatcgctt tgtgggggtt gctcatggtg atcggtcggc gtggcgaacc tccgaggcgg
     1201 ggctcaccac gccttcgcct ccgccgctca gtcgatgaag ctcaggagtt cggcgcggag
     1261 gtcgatcgtc tggagcgcgt tcggcttcca aacgacggtg ttgctgaccc cgaaggcttc
     1321 tgacgggaac cagagcaccg tgcctggcgc ctcctcttcc cacaggtcga gcagccgctg
     1381 cgagagctcg aagcgctcct ggatgccctg cgcgatctcg agttcgtgcc ccacctcgtt
     1441 gaagccgttc cggggcgtcc agccgcggat ctgttgtgtg ccggtctcgt tccacagcag
     1501 ccagagcccg cccgctggat cggggaagcg catggtgttc gaccacgtgc cgacggaggc
     1561 cgtgttgagg tctagttgag acgggtcgac cacggagacg tgggcgtcga tgccgaggtc
     1621 ttgccacatc tggacgatga tctctgccac cggaagctcg ttgacgtaac ccgtgtgacg
     1681 cgcgaggtag tagaccggtg agccgtcgta gccgctctcg gcgatcagct gcctggccct
     1741 gtcgggatcg tagggctgta cctgtcgctc ggcgttgaac aggggaccgt aagccgcgaa
     1801 ctggtggctg cgcggcactt cggcgtcgcc atgccaaagc gcatcgacga tgagttgcct
     1861 gtcgatgccg aggttcaggg cctgacgcag cttggggtcc gttatgggcg agtcccacgt
     1921 gttgtagacg agcatgtgga tgttgttgat gagcgtgcgc tccacgcgca ggtcggcgtt
     1981 gcgctccacg ctcgcgtact ggtcggcagg gagggcggcg acgatatcga cttcgccggc
     2041 gatgagggcg gccacacgcg tgctcacctc ggggatgatg cggtagacga cgcgctcggc
     2101 cgccggtcgc gcaccccagt atccggcgaa ggccgtaagc tcgatcctgt cgggctgccg
     2161 gtcactcaag cggtagaagc cggtgccgac gggttcgatg ccgaaggcat ctgcgccgac
     2221 ggcctggtaa tgcttcgcgg gcactatcca cgcgccccag aggctggcca gtcggtactc
     2281 gaggatcggg tccggctgcg cagtgctgat gcgtaccgtg tgatcgtcga cgatgtcgac
     2341 cgcggttatc gtcgcgaaca ggctgcgaga gaggctgcct cgatagtcgg ggttcatgaa
     2401 gcgctcgaag ctgaacttga cgtcttcggc gccgaacggg tcgccgttgt ggaacgtcac
     2461 gccttcgcgc aaggtgagtt cgagcgtgag gtcgtcgacg cggtgccagc tcgtggcgag
     2521 cttgggcttg aggacgctgt tgtcctgcat gtccatctcg atgagcgtct cgaagaggtt
     2581 ggggacgatc tgcatcccgg tgttgctggt ggctatgccg ggctcgaggt tggcgtcgat
     2641 gcccgcgacg gcgatgtgga ggtcgcgagc tagggcgaac agggcgagct gagcgaacaa
     2701 caacgtgagg agaagtttgc gcgaaggcat agaggcgtct gttcctttca gaagtgcgag
     2761 cgggggagcg ggaccgggtg ccggtgagtg ggtagaccct ggcggtcggc gggcttcacc
     2821 tcctcgtgag cttgcggcgg gccctcgggt cgaggcggtc gcgcaaccag tcgccaagca
     2881 gcgcgacgct gagggtcagg gagacgagca gggcgcaggg agcgagcgcg atccaccagg
     2941 cgcccatcag gtggtcgcga cccaccccga tgagcaggcc cagggaggtg gccggtggct
     3001 gtatgcctac gcccaggaac gagagggacg actctaggaa gatgaccccc gtgaagctcc
     3061 cgctggcgag gaccatcagg atcggcgccg tggtgggcag taggtgcctg aatgcgatgc
     3121 gcagttggcc gaggccgagc gctcgcgctc cctcgacgaa gtcctgacgc ttggcggcga
     3181 gcagttgccc tctcaccaac cgagcaacgc cctcccagcc ggccagcccg accacgagga
     3241 ggaagacccg gatgtcggtg ccgagggaag ccatcagcac tagcacgacc agggtgtacg
     3301 ggacggacat ctgggtctct acgaggaaca tgacgacggc gtccgctgcc cgcccgccca
     3361 gcgccgccag cgcgccgagg gcggtgccga tcactagtga gacgaccgtt ccgatggcgg
     3421 cgacgatcag tgaggtccta aggccgtaga cggtccgagc caggacgtcg cgcccgatgt
     3481 ggtcggtgcc gagcgggtgg ttcgcatggt tgcctatcca gaccggggcg gtgaggcgcg
     3541 ctctcaggtc ctgtcgcgcc gggtcgtggc cgatgaccga tgggccgagt agcgccaggc
     3601 cggcgaggag cgcgaggttg atgacctggg cgcggatgag ccatggcatc gaggtcggga
     3661 agaggcgtgg gagcctgcgt gaccgccgcg ttgggtcgtg gcgcgtcgtc ggttcccgcg
     3721 cggccgtctc cgctgctgtc gtgaccatgg ctagcctccc agccgcactc gcgggtcgac
     3781 catcgggtag agcaggtcga cgagcaggtt gcaggcgatg acggtgcccg ataccaagag
     3841 gatcgaggtt tgtaggagcg ggaagtcgcg cgtgttgacg gcggccacga tggcgcgtcc
     3901 tagcccgggc catgcgaaca ccgtctccac gacgacggtc cccgccacca gggcgccgag
     3961 ctgcgtcccg aagaccgtga ggagcggtat ggcggcgttg cgcatggcgt gcttgccaac
     4021 ggtgccccag cgcctcaacc ccttcgcccg cgcggtccgc acgaagtcct ggtcgagcgt
     4081 ctccaggagc gagccgcgta cgaggcgcac caggccggcc gcccccagca gggtcagggt
     4141 cgcgaccggc atgacgaggt tgcgccatgt gcccgtcccg ctggtcggga ggatcctgag
     4201 gaccacggag aacatcaaga tcatgccgat ggcgacgacg aagctgggga ccgcggagac
     4261 cagcgaggcg accgcgacaa tggtccggtc caggactccg ttgtggttga cggcagccaa
     4321 gccgcctagc aggaggccga acaaccatgt accggccagc gccagaccgg ccagccgcag
     4381 cgtgttgggt aggcgctcca taaggatctc gcgaactggg cgcggctcgt agtagctggt
     4441 cgcaacctgc cctcggatca ggcccgttag gtaggtgccg tactgtgcca ggagggggcg
     4501 gtccagtccg aggctctccc tgagctcctg cctagcctgc ggcggcgcgt cctcgggcag
     4561 caaccagtgg gtcgggtcgc cggtcaacct ggccgagcag aagacgagtg tcgcgatgaa
     4621 ccagagcgtg gcggcggcga gcgcgagtcg ttgaaggagg tacttgagca tccatcgacc
     4681 tcgcggtcaa ggtaggcgcc gcttgtcagg gttgtgtatg ctccagaatc cggcgccaga
     4741 ttggtgcgcc tgctacatcc gtgtcagatg ctgcggtcct gaacatcctt taggccgtgc
     4801 gcgacatgct cgagcacgat ctccagcgcg atgagctgca gtgcgcgtgg cgtgcaggcg
     4861 ttgctcccaa gtagcggttc ttgaccgcac gtcaggatga ggcgatccgc cgcccgagcg
     4921 gccggagaga gttcgaagtg ggtcaccgcc acgttgaagc accccgcctg caccgctcgc
     4981 tcgagacccg tcacgacctc gggggcgttg cctgagtggg agatcccgat gactacgacc
     5041 tccgggctga ggaggctgca gagccgctcg atctcgacgc ggtcgaccgg atggatggcc
     5101 tcgatgccgc acgccaggag gccgaaggcg aacacggtcg ccactccggc cgagccggag
     5161 acgccaagca ccgcgacccg gcgtgacgct aggattcggc tagctagctc ctcgacgagg
     5221 tggcggtcca gcatggcgat cgtttgctcg aggccgcgcg tagcgtgtgt ctgaacgttc
     5281 gtccagatgt ctgcaccgcc ggtatcgccg tgggcgtcgg tgcttgcggt cggcggctgg
     5341 tgtttgagca ggtccacgac cagccctgac ttgaagtcct ggtagccgga gtatccgatg
     5401 gagcggcaga accggagtac ggaggcctcg ctcgtcccac actgctgtgc caaagcttgc
     5461 agagacagca acgccacttc ggtgggcctg gtcctgatga ggtcggcgac cgcccgctct
     5521 gagcggctca ggcgagggta ggcgtgctgc gtgcgccgga gcgcctcgac ggttcgcatc
     5581 cgccgtgatg gtagcaccgt gcggtggggc catcgatgcc gcgtatgcgc tgggcatgcc
     5641 tgggacgggc ccgggaacga ggcggtgtgc ggggcacgct gctcgagacc tcgagagagg
     5701 gcctctctgc tacgccgccg agtagacatc ctgtagcgac caggccagga cgggcgcctt
     5761 gtaagcgttg gctgcgtcgg cgaagcgttc ggtgaacccg ctggccgaca tgaagatcaa
     5821 aggcccgtcg ttcatggccc ggtgcgccca cttcaagcct gcgtctgcgg cgcgctggag
     5881 cgcggcgagg tgatcgtaga agacggaggg tcctatcggg gtccgagagt acttgacggc
     5941 cccggtcatc aggcgcttgt cgacgagcgg ggcgaccacg tcgacttcca gtgatcgccc
     6001 gctgcggtcg cggccttccc atctggacca acgttcgacg aggggaagca ctcgcgggtc
     6061 gcgtagccgc tcgtacgttg gagcgacgag gtcctcgaag gccaagccca tgaacgtgtc
     6121 gaggcggggc gcgactactt gggcgtagac ctggcggggg tcggagcgct ctagcagcga
     6181 ggtgtacggc gagacgaaca cgtgaaagag acgcagcgtg cggtcggcca agcggtaacg
     6241 ggtgggggcg ttgggtgcct tcgcgtgcca aaagtaggtg ggccacgttc tcggccggac
     6301 ccgccttcgg gtccatcgcg gcccaatagg cgggggtccc tccgaggatg ccgtagcc
//
LOCUS       DAOQYE010000014         6195 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_12980_length_6195_cov_2.746417, whole genome shotgun sequence.
ACCESSION   DAOQYE010000014 DAOQYE010000000
VERSION     DAOQYE010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 6195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6195
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12980_length_6195_cov_2.746417"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="PKN52_00665"
     CDS             complement(<1..268)
                     /locus_tag="PKN52_00665"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNQ98475.1"
                     /translation="MNSQRLTLLTIVVAVLVVLAAVIVPRLTSSSAAPFATIDYASQP
                     HAGSADAPVKVAVFIDFLCPHCADFSENVAPQLLREFADTGKAAF"
     gene            294..1307
                     /locus_tag="PKN52_00670"
     CDS             294..1307
                     /locus_tag="PKN52_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdH/YhfP family quinone oxidoreductase"
                     /protein_id="HNQ98476.1"
                     /translation="MATFVPPDTYRSLRVTLENGAARREVVDAPWSTLPDHDVTVRVS
                     HSSLNYKDALSASGNRGVTRDYPHTPGIDAAGTVAASRDPRFAEGDPVLCTGFDLGMN
                     TAGGLAEYVRVPGDWLVRRPTGLSAHDAMAFGTAGLTAALAVARLAAVGVTAALGPAV
                     VTGASGGVGTLAVALLAAEGYEVVASTGKEAAAGLLQRLGAARTIPRVELASESSRPL
                     LPTRYAGGVDTVGGITLANLIKSTAIGGAVAACGLVGGSDVPLTVFPFILRGVALLGV
                     DSQHAGMAARQAVWELLATDRQLLGIVQDPELVREMGLDELEEAFAALLAGGVTGRII
                     VRL"
     gene            1416..2027
                     /locus_tag="PKN52_00675"
     CDS             1416..2027
                     /locus_tag="PKN52_00675"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HNQ98477.1"
                     /translation="MAFKLPDLPYATDALEPNIDARTMEIHHDKHHAAYTNNLNAAIE
                     KHPELADKSAEDLVKNLAQVPDDIRTAVQNNGGGYLNHNLFWEIMGPKGGGEPSGALA
                     SAINDAFGSFSAFQEKFAAAAAGRFGSGWAWLVVGKDGALKVYSTANQDSPLMQGDTP
                     VLGLDVWEHAYYLNYQNRRPDYIKAFWNVVDWNAVGKKLASAR"
     gene            2096..2596
                     /locus_tag="PKN52_00680"
     CDS             2096..2596
                     /locus_tag="PKN52_00680"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HNQ98478.1"
                     /translation="MRALRRDLLIRVLVLVGLCLAAALGVGTFARRPAPPLVVRPPIT
                     VAVSGEVVRPGMYEVEFGARVEELLRLAGGFLPTAARSLVQLAAPLTDGQSVVVPALA
                     TPGGTERVSLNSADPARLETLPGIGPATARRIVEHRPYARIDDLLRVPGIGPKKLERL
                     RALVTL"
     gene            2593..4929
                     /locus_tag="PKN52_00685"
     CDS             2593..4929
                     /locus_tag="PKN52_00685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HNQ98479.1"
                     /translation="MTGTFVPWPLPLAVGVVLGVVVTAPLGATATWSLASVLAAVGAL
                     AWRRGGWRWPHGLADTGLAGLTPFLPVVALAAAVGAARMQAWQAGPTERQRAVLAAEH
                     AGVESEWRGRSDGEFLHATAPMAARLAIVMPAGVPTPVGEVVLTGTAEPAPGKRNPGG
                     FDYAAHLARRGVAGQLFVRTAGGGSSGTLRLVDRLAVGVAAGLPDRAAGLMLALTLGK
                     RDDLGDLRDAFGAAGMAHLLALSGLHVGVLLLALGRSVSRLPRARLPVLAAATVAFVL
                     LVGPSPSVVRAATMSLAALASAALGAGRAHAWATLGLAALVGTLTAPQMVFDLGFQLS
                     YLAVAGMLVFLPPWTRGLAPRGTGGRSRRGGAPSSVRGRAAWLVAAIASGGLVSVAAQ
                     LPTLSLVAGTFGGVPVASPLVNVVAVPLAGLLVPLGFLAAVAGLVATPLAALVNLMTG
                     PLAAALVWTAELGARLPSVSWGEIGPVGHVAWVGACVGIAACSHGRLRAPQALAVLLI
                     AGVGTFALTPRQAVPDIWFLDVGQGDAALIRLPGGAGVLIDGGGTPFSDYDVGRRVVL
                     PALRALGVRRLDAVVSTHPDADHIEGLMTVLAAMPVGLLITGPPAPEVALDVRLRDLA
                     AAREIRVHEAWRGEHLVVGGGRVVLDVLNPARGPDPPSGNDASVALVLRVDGVARALF
                     LGDLGVAVEPELALPPVDVLMVGHHGSRGSTSEGLVRAASPSLAVVSVGRNAYGHPTT
                     EVLERLQAHGATVLTTLERGAVRVGLRGSTTWSSMVGP"
     gene            5031..5786
                     /locus_tag="PKN52_00690"
     CDS             5031..5786
                     /locus_tag="PKN52_00690"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNQ98480.1"
                     /translation="MLLTVRVLSEQGPVRVELARWEDRLVVVKRLLGTHPVASQRLER
                     EAAVVGRLRHENIVPLLGISGDASLIYAYCPGVTLEAALERGPLRPRRAMKIARDVLS
                     ALAYAHTQGVLHLDVKPANILVKGETALLTDFGFAKDLGLTAITAQDMLLGTPSFMAP
                     EQFKGVRTDHRSDLYATGAVIYHMLTGAPPFGGQVVRWLAGDDRVPLDPLPESVAQYG
                     PIVAKALSRDPDDRYSCAEEFLAALEDVALAAT"
     gene            5797..>6195
                     /locus_tag="PKN52_00695"
     CDS             5797..>6195
                     /locus_tag="PKN52_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98481.1"
                     /translation="MISSFTGDPFLARRAARAAIHEAGLDPAGVTELGEGMTAQTVLE
                     LASQGGLFTQAALLLDFDAAFQGQAGVKPRNEVMKALESVGDGAVVFVLDPSATPARQ
                     KALQALGKHTNLALPRGDQLARWVGNELKAA"
BASE COUNT          813 a         2248 c         2223 g          911 t
ORIGIN      
        1 agaaggcggc cttgccggtg tctgcgaact ctcggagcag ctgcggcgcc acgttctcgc
       61 tgaagtccgc acagtggggg cagaggaagt cgatgaagac ggctaccttg acgggagcgt
      121 cggcactgcc tgcgtgaggc tgcgaagcgt agtcgatggt ggcgaagggc gcggccgagg
      181 acgaggtcag gcgcggcacg atgacggcgg ccagcaccac gagcacggct acgacgatcg
      241 tcaacaacgt cagacgttga gagttcatgg tgcgcagtct ataggattga cccatggcta
      301 ccttcgttcc ccccgatacg taccgctcgt tacgtgtgac gctggagaac ggcgcggcgc
      361 ggcgcgaggt ggtcgacgcg ccttggtcga cgctgcccga ccacgacgtc acggtgcgcg
      421 tgagccactc cagcctcaac tacaaggacg ccctctcggc cagcggcaac cgcggggtga
      481 cccgcgacta cccgcacacg cccggcatag acgccgccgg caccgtcgcg gcctcccgcg
      541 acccccggtt cgccgagggc gaccccgtgc tctgcacggg cttcgatcta gggatgaaca
      601 ccgcgggcgg gctcgcggag tacgtgcgcg tacctggcga ctggctcgtg cgccggccga
      661 ccggcctgag cgcgcatgac gcgatggcgt tcggcacggc cggtctcacc gcggcgctcg
      721 ccgtcgctag gctggcggcg gtcggcgtca cggctgcgct cgggccggcc gtcgtaacgg
      781 gtgcgtccgg cggcgtcggg accctggccg tggccctctt ggccgccgag ggttacgagg
      841 tcgtggccag cacgggcaag gaagccgcgg ccggactgtt gcagcgcctc ggcgcggcgc
      901 gcaccatccc ccgggtggag ttggcaagcg agtcgtcgcg gccgctactg ccgaccaggt
      961 acgcgggcgg ggtggacacg gtcggcggga tcacgctcgc caacctcatc aagagcacgg
     1021 cgataggcgg cgccgtcgcc gcatgcgggc tcgtcggcgg gtcggacgtg ccgctgaccg
     1081 tgttcccgtt catcctccgc ggcgtcgcgc tcctcggcgt cgactcgcag cacgccggca
     1141 tggccgcacg acaagccgtc tgggagctgc tcgcgacgga ccgacagctg ctcggcatcg
     1201 tccaggaccc ggaactcgtg cgggagatgg ggctggacga gctcgaggag gcgttcgcag
     1261 cgctgctcgc cggcggcgtg acgggccgga tcatcgtccg cctgtaacgc ctcgaccggc
     1321 gcgcggcgcc cgcgctgcta cactcgcgtg acgtagtcgc gccgacctcc ctgccgggac
     1381 gggtcgccgc cgaacatcga aaggagcccc taaccatggc cttcaagctt cccgacctgc
     1441 cgtacgccac cgacgctctc gagccgaaca tcgacgctcg cacgatggag atccaccacg
     1501 acaagcacca cgcggcctac acgaacaacc tgaacgccgc tatcgagaag cacccggagc
     1561 tcgccgacaa gagcgccgag gacctcgtca agaacctcgc gcaggtgccg gacgacatcc
     1621 gcacggccgt ccagaacaac ggcggcgggt acctcaacca caacctcttc tgggagatca
     1681 tgggcccgaa gggcggcggc gagccgagcg gcgccctcgc gagcgcgata aacgacgcgt
     1741 tcggctcctt ctccgccttc caggagaagt tcgcggctgc cgcagcaggc cgcttcggtt
     1801 ccggctgggc atggctggtc gtcggcaagg acggtgccct caaggtctac tcaacggcca
     1861 accaggactc gcccctcatg cagggcgaca cgcccgtcct cggcctcgac gtctgggagc
     1921 acgcctacta cctcaactac cagaaccggc gccccgacta catcaaggcg ttctggaacg
     1981 tcgtcgactg gaacgcggtc ggcaagaagc tcgcctcggc gcgctgaccc atagcctcta
     2041 ggcacgagcc cacgcggacg ttagttccgc gtgggcttct tcgtagcgtc gcgctatgcg
     2101 tgcgctgcgc cgagatctcc tgatcagggt cctcgtgctc gtcggcctct gcctggcggc
     2161 cgccctcggg gtgggcactt tcgcccggcg gccagcgccg ccgctggtcg tgaggccgcc
     2221 catcacggtg gcagtgtcgg gcgaggtcgt gcggcccggc atgtacgagg tcgagttcgg
     2281 ggcgcgtgtc gaggagctgc tgcggctggc cgggggcttc ctgcccaccg ccgcgcgcag
     2341 cctcgtgcag ctcgccgcgc cgctcacgga cgggcagagc gtcgtcgtgc ccgcgctcgc
     2401 cacccctgga gggaccgagc gcgtctccct caactccgct gaccccgccc ggctggagac
     2461 cttgcccggc atcggtccgg ccacggcgcg gcgcatcgtc gagcaccgac cctacgcgcg
     2521 catcgacgac ctgctgcgtg tccccggtat cggccccaag aaactggagc ggctgagggc
     2581 gctggtgacg ctgtgacggg cacgttcgta ccctggccgc tccccctcgc cgtcggcgtc
     2641 gtgctcgggg tggtcgtcac ggcaccgctc ggtgcgacgg ccacgtggtc gctcgcctcc
     2701 gtgctagcag cggtcggcgc gctggcctgg cgaaggggcg gctggcggtg gccacacggg
     2761 ctggcggaca ccggcctcgc gggtctcacg ccgttcctgc cggtagtcgc cctcgcagcc
     2821 gcggtcggcg ccgcacgcat gcaggcctgg caggccgggc cgaccgagcg tcagcgggcc
     2881 gtcctcgccg cggagcacgc gggcgtggag tcggagtggc gcggccggtc ggacggtgag
     2941 ttcctgcacg ccacggcgcc catggccgca cgcctcgcca tcgtcatgcc ggccggcgtc
     3001 ccgaccccag tcggtgaggt ggtcttgaca ggcacggcgg agccggctcc aggtaagcgg
     3061 aaccccgggg gcttcgacta cgcggcgcac ctggcgcggc gcggcgtggc ggggcaactc
     3121 ttcgtgcgca ccgcgggcgg cgggagcagc ggcacgctcc gcctggtcga ccggctggct
     3181 gtcggggtag ccgcgggcct gccggaccgc gcggccgggc tcatgctcgc cctgacgcta
     3241 ggcaagcgcg acgacctcgg cgacctgcgc gacgccttcg gagcggccgg catggcacac
     3301 ctgctcgctc tctccggcct gcacgtcggc gtgctgctgc tcgccctcgg caggtcagtg
     3361 agccggttgc ctcgcgcccg actgcccgtg ctggccgcag cgaccgtggc gttcgtgctg
     3421 ctcgtgggcc ccagcccgag cgtggtgcgc gcggccacca tgtcgctcgc cgcgctggcg
     3481 tccgccgcgc tcggcgccgg gagggcgcat gcgtgggcga cgctgggtct cgcggcgttg
     3541 gtcggtacgt tgaccgcccc acagatggtc ttcgacctcg gcttccagct ctcgtacctc
     3601 gcggtggcgg gcatgctcgt cttcctgccg ccgtggacga gagggctggc ccctcgggga
     3661 acaggcggcc gctcccgccg tggcggcgcg ccgtcgagcg ttcgcgggcg ggccgcctgg
     3721 ctcgtcgccg cgatcgccag cggcggcctc gtcagcgtgg cggcgcaact gcccacgctc
     3781 tcgctcgtgg ccggcacctt cggcggggtg ccggtggcgt cgccgctcgt gaacgtcgtg
     3841 gcggtgccac tggcgggcct gctcgtgccg ctcggcttcc ttgccgccgt cgccgggctc
     3901 gtggccacac cgttggctgc cctcgtgaac ctgatgaccg gcccgttggc ggccgctctc
     3961 gtatggacag cggagctcgg agcgcgtctc ccgtccgtct cctggggtga gatcggcccg
     4021 gtcgggcacg tcgcgtgggt gggcgcttgc gttggcatcg cggcctgctc gcacggccgg
     4081 ttgcgagccc cccaggcgct cgccgtgctc ttgatcgccg gtgtcggcac cttcgccctg
     4141 accccgcggc aagccgtgcc cgacatctgg ttcctcgacg tcgggcaagg cgacgccgcc
     4201 ctgatacgcc tgccgggcgg ggccggcgtg ttgatagacg gcggtggcac ccccttctcc
     4261 gactacgacg tgggcaggcg cgtggtcctt cccgccctgc gcgcgctagg ggtgaggcgc
     4321 ctcgacgcgg tcgtatcgac gcacccggac gcggaccaca tcgaggggct catgacagtc
     4381 ctcgccgcca tgcccgtcgg gttgctgatc accggcccgc ccgcgccgga ggtcgccctt
     4441 gacgtcaggt tgcgcgactt ggccgccgcg cgggagatca gggtgcatga ggcctggcgg
     4501 ggcgagcacc tcgtcgtagg tggcggtcgc gtcgtcctgg acgtcctcaa ccccgcgcgc
     4561 ggccccgacc cgccgtccgg caacgacgcc tcggtggcgc tcgtgctgcg cgttgacggc
     4621 gtcgccaggg cgctgttcct tggcgacctc ggcgtcgccg tcgagcccga gctggccctg
     4681 ccacccgtcg acgtcctcat ggtcggacat cacggctcgc gcgggagcac gagcgaaggg
     4741 ctggtgcgcg ctgcgagccc gtcactcgcg gtcgtctccg tcggcaggaa cgcgtacggg
     4801 caccctacga ccgaggtcct ggagcgcctc caggcgcacg gcgcgacggt gttgacgaca
     4861 ctggagcgcg gcgcggtgcg ggtgggcctg cgcgggagca cgacgtggtc gagcatggtg
     4921 gggccgtgag gtccggtcgg gcgggagggc tcgactggtt gctgaggttc gcccgcgtgc
     4981 gtcggcaaac tcggcggccg cgcgcggagg tgcgtgatag attgcacaca gtgctgctaa
     5041 cggtccgcgt gctatcggag caaggtcccg tgcgggtaga gctcgcacgc tgggaagacc
     5101 ggctcgtggt cgtcaagcgc ctcctcggca cgcacccggt cgccagccag cgcctggagc
     5161 gggaggccgc cgtcgtcggc aggctcaggc acgagaacat cgtccccctg ctcggcatct
     5221 ccggcgacgc gtcgctcatc tacgcttact gccccggcgt gacgttggaa gccgccctag
     5281 agcggggacc gctgcgcccc cgccgggcca tgaagatcgc gcgggacgtc ctgagcgccc
     5341 tcgcctacgc ccacacccag ggagtcctgc accttgacgt gaagccggcc aacatcctcg
     5401 tgaagggcga gacggccctc ctgacggact tcggcttcgc caaagacctg ggcctcacgg
     5461 ccatcaccgc ccaggacatg ctcctcggca ccccgagctt catggcgcct gagcagttca
     5521 agggcgtgcg caccgaccac cgctcggacc tgtacgccac gggcgcggtc atctaccaca
     5581 tgctcacggg ggcgccgccg ttcggcgggc aggtcgtgcg ctggctcgcc ggcgacgacc
     5641 gggtgccgct cgaccctctg cccgagtcgg tcgcccagta cggcccgatc gtcgccaagg
     5701 cgctgtcgcg cgaccccgac gaccggtact cttgcgccga ggagttcctc gccgcgctag
     5761 aggacgtcgc cctggccgcg acgtaagctc gacccgatga tcagctcatt caccggcgac
     5821 ccgttcctgg cgcgccgcgc cgcacgcgcg gccatccacg aggccggcct ggacccggca
     5881 ggggtcacag agctgggcga aggcatgacg gcgcagacgg tgctggagct cgccagccaa
     5941 ggcggcctgt tcacgcaggc tgcgctcctg ctcgacttcg acgccgcgtt ccagggccag
     6001 gcgggcgtga agccccgcaa cgaggtcatg aaggcccttg aatcggtcgg tgacggcgcc
     6061 gtcgtgttcg tcctcgaccc gagcgcgacc cccgcgcggc agaaggccct gcaggccctc
     6121 ggcaagcaca cgaacctcgc gctgccacgc ggcgaccagc tcgcgcggtg ggtggggaac
     6181 gagctgaagg cggcc
//
LOCUS       DAOQYE010000015         5983 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_13546_length_5983_cov_3.786269, whole genome shotgun sequence.
ACCESSION   DAOQYE010000015 DAOQYE010000000
VERSION     DAOQYE010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 5983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5983
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13546_length_5983_cov_3.786269"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /locus_tag="PKN52_00700"
     CDS             complement(<1..629)
                     /locus_tag="PKN52_00700"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNQ98482.1"
                     /translation="MLERLEPIDREQGAGRTLREQLVIVLRKAILEAALPPGERLVET
                     ALADQLGVSRGPLREAIRQLVEEGLVDQAPFTGAVVRSLSLEDIQEIYSFRTLLESFA
                     FKIVWDKRTPEFYRMLDSRHRALAYAIYSGDQQQAITREMDLHGLVYEFSKHRSLIDS
                     WNLLRRRVHFYFTLHHEAHHRKGALPEAHDTYVALAKGDDLDAMLREVE"
     gene            complement(762..2330)
                     /locus_tag="PKN52_00705"
     CDS             complement(762..2330)
                     /locus_tag="PKN52_00705"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNQ98483.1"
                     /translation="MNIPKKLIALLVLVLAGLGLAQQSGGAIIVGTGNLPTTLDSGDA
                     NDGNSIAVTSQITEKLITHAPGTTNLIPALAKSWSGNDDSTVWTFNLEQGVRFHDGTP
                     FNAEAVKFTLDRWNDLDNPYHFAEAGKGYPGWVALFGGFHGSGSVLEKVTVVDEYTVQ
                     LELNRSLSFLPAILASGYFGFDSPTAVMAAGADYGTPKVGSVGTGPFMFGLWEEGTRI
                     VLNANPDYRKGPPASAQLVFVGVGDATARIAQLKAGALDVVYALSQGDLPSLENDPNI
                     EIIRSSTGGLNTSMLSMHQSQPPFDDVRVRQAVSYAIDRQALVDAFDNGEVPAAKDIL
                     PPNLWGHAGVEPYTYDPDKARALLAEAGYPNGFDTELWYRETARFRLGMVEAMASYLA
                     EVGIRASLKTEDAAAFLQDQLAGKFPIYTAGWNADFADPDTFIYTFYGPTAMQRFGWD
                     APDVVALTEEARKAPTQTERAVLYAEVMAIAHEAAPSLPLAHGTPFMAIRKGVEGVVI
                     NPLGGLPPMEGVSK"
     gene            2457..4232
                     /locus_tag="PKN52_00710"
     CDS             2457..4232
                     /locus_tag="PKN52_00710"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HNQ98484.1"
                     /translation="MRESSVGERLDRLRSMMAKRGIQAWIATTGDAHLSEYIGARWQT
                     RAWLSGFRGSAGRLVVTRDFAGLWVDSRYHQRADADTEGTPITVFKEGRPGTPELTAW
                     LATNLPVGAVVGYDPETLSMEAFAEYQARLSPAGLEPRPAHGLIDEVWPDRPEEATAP
                     IFAHPLEYAGETAESKLARLRKRVAGAGADAVLLTALDEIAWLLNIRGNDVPLNPVAL
                     AFCLVDAASARLYVHLDRVDDALRASLPNEVEVCDYRAVAEGLAGLQATTALLLDPTR
                     TSVALAQAAGHVRLVPHPSPVAHLKAVKNRTELAGAAAAHLQDGIGLTRLLHWLSECD
                     VAAESEASVARKLEAFRAGLPEYRGPSFNTIVAYGPHSSEGHYRTDPEDRQPLGTDGP
                     LLIDCGSHFRYGTTDTTRTVALGDPTPEQRLAYTLVLKSLIGLSSARFPKGTVGKQLN
                     ALARAPLWRAGLDCPHGIGHGVGSYLHVHEGPQRIHSRNEEPFGPGMVNSCEPAVYFE
                     GRFGVRLENVIVTSQDETNAFGEFYRFDTLTLCPFDPALIETGMLTKDEQAWLNEYHG
                     RVERSLSPHLTERERAWLRQKTAPV"
     gene            complement(4335..5294)
                     /locus_tag="PKN52_00715"
     CDS             complement(4335..5294)
                     /locus_tag="PKN52_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HNQ98485.1"
                     /translation="MTTASSSSWGTHDALPDDRNKDVLIYVNGELAPRAEAKVSVFDS
                     GFLVGDGVWEGLRLQGGVLLFLEQHLRRLYEGAKTIALDIGLTPEQLTAELWKVLDAN
                     GMKDGVHVRLMITRGSKRTPSQDPRFVVSGPTIVIIAEYKSSNPDVRATGVKLFTSSV
                     RRPSPDFLDPKLNCHSKLHEVIALHQALLAGADEALMLDPHGAVSTCNATNFFCVRGD
                     EVWTSTGQYCMNGITRGTVIRLCKENGIPVFERDFSLTDVYGADEAFVTGTFGGLTPV
                     AVVDGRGIGKGIPGPVTTRLTELYVAEKTRYVGERQTVGAERG"
     gene            complement(5429..>5983)
                     /locus_tag="PKN52_00720"
     CDS             complement(5429..>5983)
                     /locus_tag="PKN52_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ98486.1"
                     /translation="TALGVLAAIMRGWLDLTLSWFIDILLAFPGILLAIAIVAALGAN
                     LTNALVAISIMGIPGYYRVMRSAIIGLRDLEFVQAAEALGSSKFRVAVKHLLPNALSP
                     LIVQVTLAIGRTVLSIASLGFLGLGAQPPTPEWGLMIKSGYSQFLTAPWLSIAPGFAI
                     YLAVLGFNMLGDAIRDHFDPQLRT"
BASE COUNT          979 a         1969 c         2087 g          948 t
ORIGIN      
        1 tcctcgacct cgcgcagcat ggcgtcaagg tcgtcgccct tggcgagggc cacgtacgtg
       61 tcgtgcgcct caggcagggc gcctttgcgg tggtgggcct cgtggtgcag cgtgaagtag
      121 aagtggacgc gtcttcgcag caggttccac gagtcgatga ggctgcggtg cttagagaac
      181 tcgtacacga ggccgtggag gtccatctcg cgcgtgatgg cctgctgctg gtcgccggag
      241 tagatggcgt aggccagcgc ccggtgccgc gagtcgagca tccggtagaa ctcgggcgtt
      301 cgcttgtccc acacgatctt gaaggcgaac gactcgagga gggtgcggaa ggagtagatc
      361 tcctggatgt cctcgaggga gagcgacctg acgaccgccc cggtgaaggg ggcctggtcg
      421 accaggccct cctccaccag ctgccggatg gcctcccgca gcggaccccg gctcacgccg
      481 agctggtcgg ccagcgcggt ctcgacgagg cgctcgcccg gcgggagggc tgcctccagg
      541 atcgccttgc gcagcacgat gacgagctgc tcgcgcaacg tcctgccggc cccttgctcc
      601 ctatcgatcg gctccagccg ttccagcact tactacctcc aggcgccgcc tcacacgcct
      661 tgctcgaaga cgggatacgc cctccaggag cacgacctga tttcggtgcc gcacgctgtt
      721 ctgaccgtac gataccgttc cggcggcacc ggaatgctct cttacttgct gacgccctcc
      781 atgggcggta gtccaccgag cgggttgatg acgactccct cgacgccctt tcggatcgcc
      841 atgaacggcg tgccgtgtgc gagggggagc gagggcgcgg cctcgtgagc tatggccatg
      901 acctccgcgt acagcacggc gcgctcagtc tgcgtcggag ccttgcgggc ctcctcggtc
      961 agcgccacga cgtcgggggc gtcccagccg aagcgctgca tggcggtcgg gccgtagaac
     1021 gtgtagatga acgtgtcggg gtcggcgaag tcggcgttcc agccagcggt gtagatgggg
     1081 aacttgccgg ccagctggtc ctgcaggaac gcggcggcgt cttccgtctt gagcgaagcg
     1141 cggatgccga cctcggcgag gtaggaggcc atcgcctcga ccatgcccag ccggaacctg
     1201 gcggtctcgc ggtaccagag ctccgtgtcg aagccgttcg ggtaaccggc ctcggccagc
     1261 agcgcccgcg ccttgtcagg atcgtaggtg tacggctcga cgccggcgtg accccagagg
     1321 ttgggtggca ggatgtcctt cgcggccggc acttcaccgt tgtcgaacgc gtccactagc
     1381 gcctgcctat cgatggcgta ggaaacagcc tgcctcacgc gcacgtcatc gaacgggggc
     1441 tggctctggt gcatcgagag catgctcgtg ttcaggccgc ccgtgctgct ccggatgatc
     1501 tcgatgttcg ggtcgttctc caaggacggc aggtcgcctt gcgagagcgc gtacacgacg
     1561 tcgagcgcgc ccgccttgag ttgggcgatg cgcgcggtgg cgtcgccgac gccgacgaag
     1621 acaagctgcg ccgaggccgg cgggcccttg cggtagtcgg ggttggcgtt caggacgatg
     1681 cgagtgccct cttcccatag gccgaacatg aacggaccgg taccgacgga gccgaccttc
     1741 ggcgtgccgt agtccgcccc ggcagccatg acggccgtgg ggctgtcgaa gccgaagtag
     1801 ccggaggcga ggatggcggg gaggaacgac agggagcggt tcagctcgag ctgaacggtg
     1861 tactcgtcga cgacggtcac cttctcgagc acgctgccgc taccgtggaa accgccgaag
     1921 agcgcgaccc agccggggta gcccttgcct gcctccgcga agtggtacgg gttatcaagg
     1981 tcgttccacc ggtcgagggt gaacttgacg gcctcagcgt tgaacggcgt gccgtcgtgg
     2041 aagcgcacgc cctgctcgag gttgaaggtc cataccgtcg agtcgtcgtt gcccgaccag
     2101 ctcttagcca gtgccggaat caggttcgtg gtacctggtg catgcgtgat gagcttctcg
     2161 gtgatctggc tggtgacggc gatcgagttg ccgtcgttcg cgtcgcccga gtccagcgtc
     2221 gtcggcaagt tgcccgtgcc gacgatgatg gccccgccgg actgctgcgc gaggcccaga
     2281 ccggccagca cgagcaccag caatgcgatc agtttcttgg ggatgttcat tgcgctccta
     2341 acgcaccgcc cggcgagctt cgacccgtct tcgatggacg tggcggcacc gtggccggtc
     2401 agttggatgg ctttccagcg tgagggtagc agcgcccatg ctagattgtc aacagtatga
     2461 gagaaagttc ggtcggcgag cggctcgacc gcttgcgctc catgatggcc aaacgcggca
     2521 tccaggcctg gatcgccacc accggagacg cgcacctgag cgagtacatc ggcgcgcggt
     2581 ggcagaccag agcctggctc agcggcttcc gcggctcggc gggcaggctc gtcgtcacac
     2641 gggacttcgc gggcctgtgg gtggactccc gctaccatca gcgcgccgac gccgacacgg
     2701 agggcacgcc gataacggtg ttcaaggaag gcaggccggg cacgccggaa ctgacggcgt
     2761 ggctcgctac caacctgccg gtgggcgccg tcgtcggcta cgaccccgag acactgagca
     2821 tggaggcgtt cgcggagtac caggcgcgcc tctccccagc cgggctagag ccgcggccgg
     2881 ctcacggcct gatcgacgag gtctggcccg acagaccgga agaggccacg gcgccgatct
     2941 tcgcccaccc cctcgagtac gcgggtgaaa cggccgagag caagctcgcg cggttgcgca
     3001 agcgcgtcgc gggagccggc gcagacgccg tcctgctcac cgcgctcgac gagatcgcat
     3061 ggctgctcaa catccgcggc aacgacgtcc cgctgaaccc ggtggccttg gcgttctgcc
     3121 tggtggacgc agcatccgcg cgcctctacg tgcaccttga ccgcgtcgac gacgcgctca
     3181 gggcctcgct gcccaacgag gtggaggtct gcgactacag ggcggtggcc gaggggctcg
     3241 ctggtctcca ggccacgacg gcgctgctcc tggacccgac caggacgagc gtcgccctag
     3301 cacaagccgc cggacacgtc aggctcgtgc cgcacccgag cccggtggcc cacctcaagg
     3361 cggtcaagaa ccgcacggaa ctggccggcg cggcagccgc ccacctgcag gacggcatcg
     3421 gcctcaccag gctcctgcac tggttgagcg agtgcgacgt ggccgcggag agcgaagcga
     3481 gcgtggcgcg caagctcgag gctttccggg cagggctgcc cgagtaccgc ggcccgagct
     3541 tcaacaccat cgtggcgtac ggaccgcact cttccgaggg ccattaccgc accgaccccg
     3601 aggaccggca gccgctcggc acggacggcc cgctgctcat cgattgcggc tcccacttcc
     3661 gttacggcac caccgacacg acgcggacgg tcgcgctggg cgaccccacg cccgagcagc
     3721 gcctggcgta cacgctcgtc ctcaagagcc tgatcgggct gagctcggcg cggttcccga
     3781 agggcacggt cgggaagcag ctcaacgccc tggctcgcgc cccgttgtgg cgcgccgggc
     3841 tcgactgccc gcacgggata ggccacggcg tgggaagcta cctgcacgtg cacgaggggc
     3901 cgcagcgcat ccactcgcgc aacgaggagc ccttcgggcc aggcatggtg aactcctgcg
     3961 agccggcggt ctacttcgag gggcgcttcg gcgtgcggct ggagaacgtg atcgtgacga
     4021 gccaagacga aacgaacgcg ttcggcgagt tctaccgttt cgatacgctc acgctatgtc
     4081 cgttcgaccc ggccctcatc gaaacaggca tgctcacgaa agacgagcag gcgtggctga
     4141 acgagtacca cgggcgcgtg gagcgttccc tgagcccaca cctcaccgag cgcgagcggg
     4201 cgtggttgcg gcagaagacc gcgccggtat gaggccactg aacccagaga caagcgggcc
     4261 gtgagtctcg cctcgccgag aaggtaccga ccaccggtcg tcgtcgccat ccggtcaagg
     4321 cggcctggac aggctcagcc ccgctcagct ccgacggtct gtcgctcccc gacgtaccgc
     4381 gtcttctcgg cgacgtagag ctcggtcagc ctggtcgtga cgggccccgg gatccccttc
     4441 ccgatgcccc gcccgtccac caccgctacc ggcgtgagac cgccgaacgt gccggtcacg
     4501 aacgcctcgt cggcgccgta cacgtcggtg agcgagaaat cgcgctcgaa gactgggatg
     4561 ccgttctcct tgcagagcct gatgacggtg ccgcgcgtga tgccgttcat gcagtactgg
     4621 ccggtcgagg tccagacctc gtccccgcgc acgcagaaga agttggtggc gttgcacgtg
     4681 ctcacggcgc cgtgcggatc gagcatgagg gcctcgtcgg cgcccgccag cagcgcctgg
     4741 tgcaacgcga tgacctcgtg gagcttgctg tggcagttga gcttggggtc caggaagtcc
     4801 ggagacggcc gcctgacgct cgacgtgaac agcttgacgc cagtggcgcg cacgtcgggg
     4861 ttgctcgact tgtactcggc gatgatcacg atggtcggcc cggagacgac gaagcgcgga
     4921 tcctgcgagg gcgtgcgctt gctaccgcgc gtgatcatga gccgcacgtg cacgccgtcc
     4981 ttcatgccgt tggcgtccag caccttccac agttccgccg tgagctgctc cggcgtcagg
     5041 ccgatgtcga gggcgatggt cttggcgccc tcgtagagcc gcctgaggtg ctgctcgagg
     5101 aacaggagca cgccgccctg gagccgcagg ccctcccaga cgccgtcgcc gacgaggaag
     5161 ccgctgtcga agacagacac cttggcctcg gcgcgcggcg ccagctcgcc gttcacgtag
     5221 atcagcacgt ccttgttcct gtcgtccggc agggcgtcgt gggtgcccca gctggaactg
     5281 ctggcggtag tcatggtttt cctcccctga gggtgtgaga gcgaagcggt ggcggttcgg
     5341 cgccgccgcg gtcaggcgcc tcccaggtcg gttgcgctgg tggcacgcgg cagtctcggt
     5401 cgcgcaagat gcgggcggga gacaaggtct acgtcctcaa ctgcgggtcg aaatggtcgc
     5461 ggatggcgtc cccgagcatg ttgaagccga gcacggccag gtagatggcg aaacccgggg
     5521 cgatggagag ccacggcgcg gtaaggaact gactgtagcc ggacttgatc atcagccccc
     5581 actcgggcgt cggtggttgc gctcccaggc cgaggaagcc gagggacgcg atcgagagca
     5641 cggtgcgacc gatggccaag gtgacctgca cgatgagggg agagagggcg ttgggaagca
     5701 ggtgcttgac ggccacgcgg aacttcgacg agccgagcgc ctcggccgcc tgcacgaact
     5761 cgagatcgcg caggccgatg atcgcggaac gcatgacgcg gtagtaaccg gggatgccca
     5821 tgatggagat ggcgaccagc gcgttcgtca ggttcgcgcc gagcgcggcg actatcgcga
     5881 tggccagcag gatgccgggg aacgccagca ggatgtctat gaaccatgag agcgtgagat
     5941 cgagccagcc gcgcatgatc gcggcgagca ccccgagcgc cgt
//
LOCUS       DAOQYE010000016         5855 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_13931_length_5855_cov_3.189483, whole genome shotgun sequence.
ACCESSION   DAOQYE010000016 DAOQYE010000000
VERSION     DAOQYE010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 5855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5855
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13931_length_5855_cov_3.189483"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /locus_tag="PKN52_00725"
     CDS             <1..807
                     /locus_tag="PKN52_00725"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNQ98487.1"
                     /translation="DVTVPTKPDVVFSYDYDAIDTLTVLGIAVDGAPSLAGVVPAWAQ
                     LDVINIGSLFEPDYEVVNAEQPGLIIVGGRSAAAHDELAKLAPTIDLSGSGDLLADIH
                     RNVRTLSAIFDKQADGEAALAALDAKIASVRAQVATADNGMLIMVNGGSLSAIAPDNL
                     RGRFLHEVLGLTPTIADVQSATHGEPISFEFLLEHDPQWLFVIDRDAAIGTEGAQPAA
                     AVLDNELMHNTTAWKDGNVVYLNPFNWYIVLGTGLSTANAMLDEVAAAFQ"
     gene            840..1823
                     /locus_tag="PKN52_00730"
     CDS             840..1823
                     /locus_tag="PKN52_00730"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HNQ98488.1"
                     /translation="MGESKRHGLLLVAATVLVLVLAVVSVAVGVFDLSWQGLLKGGEE
                     RLNALVLLASRIPRTLAVLLVGTSMGVAGLLMQLLSRNRFVSPSTAGTVESASLGILV
                     TMLVTPNAPVMVKMLIAAAFALGGTALLMLIIWRTPLRSPLVVPLIGLMLGGVIGAAT
                     TFIAYRYNLLQSLASWTTGDFSRVLRGRYELLWVSGGLTIVAYFAADRFTVAGMGESF
                     TKNLGIDYRKVMVLGLVIVSLVTAVNVVTVGAVPFIGLVVPNVVSLLVGDNVRRAIPW
                     IAVFGAGFVLACDLLGRTLRYPYEVPVGTVIGVVGATLFLFLLLRRSSRVG"
     gene            1813..2832
                     /locus_tag="PKN52_00735"
     CDS             1813..2832
                     /locus_tag="PKN52_00735"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HNQ98489.1"
                     /translation="MSADARTLAGRPAARQLDRRRWGRFPPAVVIIVLCVIVAISIYL
                     FMTLGARGSWSFIIPFRGRKVLGMLLVGTAISVSTVLFQTVTTNRILTPSIMGFDALY
                     MLLQTCLAFFLGATGLTQLDGRMMFAIETLGLMFFAGALYYWLFVVGHRSLHLLVLAG
                     IVLGVMLRSVTNLLQRVMDPLTFAVLQDTFFASFNTFDATLLLPAGIVVGAALYGAWR
                     MRHTLDILALGREMALGLGVDYRRTVTRALAIITALVSVSTALVGPVTFFGLIVANLA
                     YALLRSYRHRVIMPAAALLAAVVLILGQTVLERVLGLGTSLSVIVEFAGGLLFIYLVL
                     RGVVR"
     gene            2829..3587
                     /locus_tag="PKN52_00740"
     CDS             2829..3587
                     /locus_tag="PKN52_00740"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNQ98490.1"
                     /translation="MITARNLTKRYGQTVVLDDVSVEIPRGGVTALIGPNGAGKSTLL
                     SLVGRLLKADGGTVEIDGVPTHGMKQSEFAKKVAILRQDNHLAVRLTVEDLVTFGRYP
                     HSQGRPTLEDRGHVEAALDYLELQDLRHRFLDELSGGQRQRAFVAMVLCQDTDYVLLD
                     EPLASLDMRHAVAMMKRMRGVTEDLGKTVVIVLHDINFAASYADTIVAMRDGRILQHA
                     VVDEVMTPGALRAIYDVDVHVEQIGGKPIGVYYS"
     gene            3687..4004
                     /locus_tag="PKN52_00745"
     CDS             3687..4004
                     /locus_tag="PKN52_00745"
                     /inference="COORDINATES: protein motif:HMM:NF025475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4282 domain-containing protein"
                     /protein_id="HNQ98491.1"
                     /translation="MDTQSFFSKLFDMSFSQYVTPSIIRIVFVLSIVLSGIVAIGIFT
                     TASSTFGAGGAILGLVLAVLGFILYIVLARMGLEVVIALFRIAENTRVIADNARRSNG
                     SGD"
     gene            complement(4102..>5855)
                     /locus_tag="PKN52_00750"
     CDS             complement(4102..>5855)
                     /locus_tag="PKN52_00750"
                     /inference="COORDINATES: protein motif:HMM:NF022100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NEW3 domain-containing protein"
                     /protein_id="HNQ98492.1"
                     /translation="SNLHLVQSFVTDAETDTSLFAGVPYTVADLAAKVDDATLAGLLT
                     DAQAAIDRALAAFPNFDAVQGELTVALATVRAARATLAASDVDAATAYDIDFRLAQKE
                     LELQVASRRALSLVTRVVAANPELTRGGTTEVTVSAYLGGNVDADNVTFAVVAPEGWT
                     VEPKEAVEPANLTNGQTAKQTFVVTVPADAPYYNPYRRNVNPFRASGDVYGVVSYEVD
                     GVPVQVTVDSREIIGVLPDISLRVTPENLVYNFLSPADLVMSVAATNYVNGPAETVLT
                     VDAPEGWTVEPASVTLSFDRKGDARGASFTLTPGEGVVEGAYDFAVRAEGDASSTDYT
                     RVISYDHIGKTYLVSSASANVQALEVAFDPNLRIGYVDGGSDRVYEGLRQIGMNVDLL
                     TEDDLTTGDLSKYDSIAVGIYSYRARPDLFAANQRLMKWVEDGGNLLVQYHRPADAWD
                     PESVPPYEIELGRVSLLSRVTAADGEVEFLEPENPIMTTPNVLTQADFDNWVKERGLY
                     FAQEWDEHYTPLFSVTDRQWPDTWDELPFLGSMLTTDYGKGRYTYTSLVLHTEIENNV
                     PGAYRIYANLLTPPAQE"
BASE COUNT          848 a         2006 c         1942 g         1059 t
ORIGIN      
        1 gacgtcaccg tccccaccaa gcccgacgtc gtgttctctt acgactacga cgccatcgac
       61 accctcaccg ttctcggtat cgcggtcgac ggcgcgccgt ccctcgccgg ggtggtgccc
      121 gcatgggcgc agctcgacgt catcaacatc ggctccctat tcgagcccga ctacgaggta
      181 gtcaacgcgg agcagcccgg cctgatcatc gtgggcggcc gctcggccgc cgctcacgac
      241 gagctcgcca agctcgcgcc taccatcgac ctctccggtt ccggcgacct gcttgccgac
      301 atccaccgta acgtgcggac cttgagcgcc atcttcgaca agcaggccga cggcgaggcc
      361 gcgctcgcag ccctagacgc gaagatcgcg tcggtgcgcg cccaggtggc caccgccgac
      421 aacggcatgc tgatcatggt caacggcggc agcctctcgg ccatcgcccc cgacaacctc
      481 cgtggccgtt tcctccacga ggtgctcggc ctcacgccga ccatcgcgga cgtgcagtcg
      541 gccacgcacg gcgagcccat cagcttcgag ttcctcctcg agcacgaccc gcagtggctc
      601 ttcgtgatcg accgcgacgc cgccatcggt acggaaggcg cccagccggc cgccgccgtc
      661 ctcgacaacg agctcatgca caacacgacg gcctggaagg atgggaacgt cgtctacctc
      721 aacccgttca actggtacat cgtgctcggc accggcctca gcacggccaa cgcgatgctc
      781 gacgaggtcg cggccgcgtt ccagtaagca gtagtaagct gtagcgcatc atcgtgaagt
      841 tgggagagtc gaagcgtcac ggtctgctgc tggtggccgc caccgtcctg gtgctcgtgc
      901 tcgccgtggt cagcgtcgcc gtaggcgtct tcgacctctc ctggcaaggt ctgttgaaag
      961 gcggcgaaga gcgcctgaac gcgctggtgc tcctcgccag ccgtatcccg cgcaccctcg
     1021 ccgtgctcct cgtcggcacg tccatgggcg tggcggggct gctgatgcag ctgctgtcgc
     1081 gcaaccgctt cgtgtcgccg tcgacggccg gcacggtcga gtcggccagc ctcggcatcc
     1141 tcgtgaccat gctggtcacg ccgaacgcgc cggtgatggt caagatgctc atcgcggccg
     1201 ccttcgcgct gggcggcacg gcgctcctga tgctcatcat ctggcgcacg ccgctgcgct
     1261 cgcccctcgt ggtgcccctc atcgggttga tgctcggggg tgtcatcgga gccgccacca
     1321 ccttcatcgc ctaccgctac aacctcctgc agagcctcgc gagctggacg accggggact
     1381 tctcccgcgt gctgcgcggg cgttacgagc tcctgtgggt atcgggcggc ctgaccatcg
     1441 tcgcgtactt cgccgccgac cgcttcaccg tggccggcat gggcgagtcg ttcaccaaga
     1501 acctcggcat cgactaccgc aaggtcatgg tcctcggcct ggtcatcgtc tccctcgtta
     1561 cagccgtcaa cgtcgtgacg gtcggcgcgg tgccgttcat cgggctggtg gtgcccaacg
     1621 tggtcagcct cctcgtcggc gacaacgtca ggcgcgccat cccctggatc gcggtcttcg
     1681 gggcgggctt cgtgctcgcc tgcgacctgc tcgggcggac cctgcgctac ccgtacgagg
     1741 tgcccgtcgg cacggtcatc ggggtggtcg gggccacgct cttcctgttc ctgttgctta
     1801 ggcgttcgtc tcgtgtcggc tgacgcccgg accctcgcgg ggcggcccgc cgcccgccaa
     1861 ctcgaccggc gccgctgggg ccgcttcccg cccgccgtcg tcatcatcgt cctctgcgtc
     1921 atcgtggcta tctctatcta cctgttcatg accttgggcg cgcgcgggtc gtggagcttc
     1981 atcatcccgt tccgcggccg caaggttctc ggcatgctcc tcgtcggcac tgccatatcc
     2041 gtatccaccg tcctcttcca gacggtcacg acgaaccgca tcctcacccc gtcgatcatg
     2101 ggcttcgacg ccctctacat gctcctccag acgtgcctgg cgttcttcct aggcgccacc
     2161 ggcctcaccc agctggacgg gcggatgatg ttcgcgatcg agaccctcgg cctcatgttc
     2221 ttcgccggcg cgctctacta ctggctgttc gtggtaggtc atcgcagcct gcatctgctc
     2281 gtgctggcgg gcatcgtgct cggcgtgatg ctgcgcagcg tcaccaacct gctgcaacgg
     2341 gtcatggacc cgctcacgtt cgccgtcttg caggacacgt tcttcgccag cttcaacacg
     2401 ttcgacgcca ccctgctcct tcccgctggg atcgtggtgg gggcggccct ctacggcgcc
     2461 tggcgcatgc gccacacgct cgacatcctc gcgctcggtc gcgagatggc cctgggcctt
     2521 ggcgtcgact accgccgcac ggtcacgcga gcgctcgcca tcatcacggc gctcgtgtcc
     2581 gtttccactg ctctggtggg gccggtgacg ttcttcggtc tcatcgtcgc caacctcgcc
     2641 tacgccctac tgcgttccta ccgtcaccgg gtgataatgc cggctgcagc gctcctcgcg
     2701 gccgtggtgc tcatcctcgg gcagacggtg ctggagcgcg tcctgggcct gggcacgagc
     2761 ctgtccgtca tcgtcgagtt cgctggcggg ctactcttca tctacctcgt gttgagggga
     2821 gtcgtcagat gatcaccgcc cgcaacttga cgaagcgcta cggccagacg gtcgtcctcg
     2881 acgacgtcag cgtcgagatc ccgcgcggcg gcgtcaccgc cctcatcggc ccgaacggcg
     2941 ccggcaagtc gacgctcctc tccctggttg gccgcctgct caaggcagac ggcgggaccg
     3001 tcgagatcga cggcgtgcca acgcacggca tgaagcagtc ggagttcgcc aagaaggtgg
     3061 ccatcctgcg gcaggacaac cacctggccg tgcggctcac cgtcgaggac ctcgtcacgt
     3121 tcggccgcta cccgcactca cagggccgcc ccaccctgga ggaccgcggc cacgtcgagg
     3181 ccgccctcga ctaccttgag ctccaggacc tgcgccaccg cttcctcgac gagctctccg
     3241 gcggtcagcg tcagcgcgcc ttcgtggcca tggtcctctg ccaggacacg gactacgtgc
     3301 tcctcgacga gccgctcgct agcctcgaca tgcgccacgc cgtcgccatg atgaagcgta
     3361 tgcggggcgt cacggaggac ctcggcaaga ccgtcgtcat cgtgttgcac gacatcaact
     3421 tcgccgcgtc ttacgccgac accatcgtgg ccatgcgcga cgggcgcatc ctgcagcacg
     3481 ccgtcgtcga cgaggtcatg acccccggcg cgcttcgcgc gatctacgac gtcgacgtgc
     3541 acgtcgagca gatcggcggc aagcccatcg gggtgtacta cagctgagaa ggccgcgtct
     3601 gccgcgtcca agacggtaat cgcacacagg tcggacgccc cggtccccta tattgcctcc
     3661 gaaacctgca acggaggcac caaggcatgg atacgcagtc gttcttctcc aagctcttcg
     3721 atatgtcgtt ctcgcagtac gtgacgccga gcatcatccg gatcgtcttc gtgctcagca
     3781 tcgtcctctc cggcatcgtg gccatcggca tcttcacgac ggcttcctcc acgttcggag
     3841 ccggcggcgc catcctcggt ctcgtcctcg cggtgctcgg cttcatcctc tatatcgtgc
     3901 tcgcccgcat gggcctcgag gtagtgatcg ccctcttccg catcgccgag aacacgcggg
     3961 tcatcgccga caacgcgcgg cggtcgaacg gcagcggcga ctgagagccc ggtcccaccc
     4021 aaccgaacca agaagccgcc ggcccatctc gagggccggc ggcttctctt ctacaagcct
     4081 ctacgaggct ttagtgctgc gtcactcctg cgcgggcggg gtgagcaggt tggcgtagat
     4141 gcggtaggcg cccggcacgt tgttctcgat ctcggtgtgc aggacgaggc tcgtgtacgt
     4201 gtaacggccc ttgccgtagt ccgtcgtgag catggagccg aggaagggca gctcgtccca
     4261 cgtgtcgggc cactgccggt cggtgacgct gaagagcggg gtgtagtgct cgtcccactc
     4321 ctgcgcgaag tacagaccgc gctccttcac ccagttgtcg aagtcggcct gagtgaggac
     4381 gttcggggtg gtcatgatcg ggttctcggg ctcgaggaac tcgacctcgc cgtcggcggc
     4441 ggtgacgcgc gagaggagcg acacgcggcc gagctcgatc tcgtaaggcg gcacggactc
     4501 agggtcccac gcgtcggcgg ggcggtggta ctggacgagc aggttaccgc cgtcctcgac
     4561 ccacttcatg aggcgctggt tggccgcgaa caggtcgggg cgggcgcggt acgagtagat
     4621 gcccaccgcg atggagtcgt acttgctcag gtcgccggtc gtgaggtcgt cctcggtgag
     4681 gaggtcgacg ttcatgccga tctgacggag gccttcgtaa acgcggtcgg agccgccgtc
     4741 gacgtaaccg atgcgcaggt tcgggtcgaa ggcgacctcg agggcctgga cgttggcgga
     4801 ggcggagctc acgaggtacg tcttgccgat gtggtcgtag ctgatgacgc gggtgtagtc
     4861 ggtgctggag gcgtcgcctt cggcgcggac ggcgaagtcg taagcgccct ccaccacgcc
     4921 ttcacccggg gtgagggtga agctggcgcc gcgcgcgtcg cccttgcggt cgaagctcag
     4981 ggtcacggag gcgggctcga cggtccaacc ttcaggggcg tcgacggtga ggaccgtctc
     5041 ggccgggccg ttgacgtagt tcgtggcggc cacgctcatg acgaggtcgg cagggctgag
     5101 gaagttgtag acgaggttct ccggggtgac gcggagcgag atgtcgggca gcacgccgat
     5161 gatctcgcgg gagtcgacgg tgacctggac aggcacgccg tcgacctcgt acgagacgac
     5221 accgtagacg tcgccggagg cgcggaacgg gttcacgttg cggcggtagg ggttgtagta
     5281 gggggcgtcg gccggaacgg tgacgacgaa ggtctgcttg gcggtctggc cgttggtgag
     5341 gttggcgggc tcgacggctt ccttcggctc gacggtccag ccttccgggg cgacgacggc
     5401 gaacgtgacg ttgtcggcgt cgacgttacc gccgaggtag gcgctgaccg tcacttcggt
     5461 cgtgccgccg cgggtgagct cggggttggc ggccactacg cgcgtgacga gcgagagggc
     5521 gcggcggctc gccacttgaa gctccagctc cttctgggcg aggcggaagt cgatgtcgta
     5581 agccgtggcg gcgtcgacgt cgctcgcggc gagcgtcgcg cgggcggcgc gcacggtggc
     5641 gagggcgacg gtcagctcgc cctgcacggc gtcgaagttc gggaacgcgg cgagggcgcg
     5701 gtcgatggcg gcctgcgcgt cggtaaggag accggcgagc gtggcgtcat cgaccttggc
     5761 ggccaggtcg gccacggtgt acgggacgcc cgcgaagagc gaggtgtcgg tctcggcgtc
     5821 cgtaacgaac gactggacga ggtggaggtt ggagt
//
LOCUS       DAOQYE010000017         5828 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_14032_length_5828_cov_2.695652, whole genome shotgun sequence.
ACCESSION   DAOQYE010000017 DAOQYE010000000
VERSION     DAOQYE010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 5828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5828
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14032_length_5828_cov_2.695652"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..4768
                     /locus_tag="PKN52_00755"
     CDS             <1..4768
                     /locus_tag="PKN52_00755"
                     /inference="COORDINATES: protein motif:HMM:NF028476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HNQ98493.1"
                     /translation="AASGLARAFEELAARGEAEVLAMDFAFLLDHDRQLLHIGYNVDT
                     EQLDANHYDLLASEARLASLVAVAKGDAPVSHWVHLNRPLGDLGGRAALLSWSGTMFE
                     YLMPELLTRRYGATLLDQSCAAAIDVQIAYAKCNGVPWGVSESGYARVDAAGNYQYHA
                     FGVPELALNRSYSNDLVIAPYASVLALRHRPRAVLENAARLAEAGALGPHGFFEALDY
                     TPERRPLGEERVVVRSHMAHHQGMTLLALTNALTGDAMVERFHADPRVATIEYLLQER
                     IPLDVPVVDDLRPEASPSAPIDVAVLDEPWDVPVDTATPLTHLLSNGAYTVMVSEAGG
                     GWSAAGEVAITRWRPDGTRDADGTWLFIHDEGEGLTWSPTRAPLPARAQGDRVEFYPY
                     KARYQREVGGILARLEVTVAKGTAELRRLTVVNRGETSRTLRVTSYAEVVLGPVAADA
                     RHPAFSKLFVESAFLPRRSAILFRRRPRSADEPALFAAHAVVCKDDLGPCEYETDRAA
                     FLGRGNDVAAAVGPRGPLRTGGGPRRADDRRAGEKSATLDPAMALAVRFDLRPHSQEE
                     LTFVTASGASSRDLTTLLDELRKPGMVDREFDLARLRSRVELQEHDLTPSAVETYQRL
                     YSAVTYPVQELRAPAATLAASTLGQPSLWGQGISGDLPIVLVSVGSAERLPLVDDVLR
                     AHAYWRRRGVKVDVVLLNLQDTAYDQALHARLRSLLVRSGSDAWLAVNGGVHLLRRDQ
                     LAPLDLVLLESAAAVVLDGEHGSLAEQLRELGRPPRPGLPLLVQGLEPMQGPSEGPAG
                     TVRLRYDNGWGGFTEDGREYVIRARRGSLPPAPWSNVIANAELGCLVTEAGMGATWAV
                     NSGENRLTPWSNDPVTDPGSEIVYLRDEETTEVWSATPQPAGGEASFEVRHGTGRTLY
                     RHVGHGLEHALEVSVAARDPVKLVRLHVRNLSGRPRRVTVTYYAEWVLGVDRRGTGLH
                     VVPDYAPERCTLLARNPYSTDFGARVAFLAGNKSPHGVTTDRREFIGRLGSYALPDAL
                     RRVGLAGAVAPGADPCAAMQLHLDLAAGASEDVLFALGQADDRDAALRLADRYRDLGA
                     YAAARRAADEAWDAVLGKVQVSTPDPGLDLLTNRWLLYQTLSSRCFGRTAFYQPGGAY
                     GFRDQLQDVVALLHAAPELAREQLLRAAAHQFPEGDVLHWWHPPAGRGVRTHISDDLL
                     WLVYTTERYVSATGDRSVLDEEAPFLEGRPLAPDVEDRYDAYRAGSESGTLLEHCRRA
                     LRKGATAGSHGLPLMGTGDWNDGMNRVGADGRGESVWLAWFLIDVIGRFRRLLDSVGG
                     HEEEAAGWAARAGAYAHACEEAGWDGGWYLRAFYDDGTPLGTAAASEWRIDSIAQSWA
                     VLSGAAPAERAATAMAAVEELCVRRQDGLIRLATPPFDRSKKDPGYIKGYVPGVRENG
                     GQYTHGAIWSAWAFARLGERGKAHALHRLLDPVLRAGDREGAERYVVEPYVMAADVYE
                     RPPHVGRGGWTWYTGSAAWFYRFGLEGLLGLERVGDELRLTPRVPTEWPGFEVRYRHG
                     SSTYVLRARPGAIPANQVVKLRDDGGEHVVDVVYEA"
     gene            complement(4902..5138)
                     /locus_tag="PKN52_00760"
     CDS             complement(4902..5138)
                     /locus_tag="PKN52_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HNQ98494.1"
                     /translation="MAYIITEPCIGVKDASCVDVCPVECIYEADDQFYINPEECIDCG
                     ACVPACPVDAIYPEMDVPDEWADYIEKNTRLSGL"
     gene            complement(5614..>5828)
                     /locus_tag="PKN52_00765"
     CDS             complement(5614..>5828)
                     /locus_tag="PKN52_00765"
                     /inference="COORDINATES: protein motif:HMM:NF015019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HNQ98495.1"
                     /translation="AWDNAKKYIEDGNHGGKGSDAHAASVVGDTVGDPYKDTAGPSIN
                     PLIKVINTVALIFAGVIAVAGGWFLF"
BASE COUNT          838 a         2064 c         2053 g          873 t
ORIGIN      
        1 cgcggcctcc gggctggccc gggccttcga ggagctcgcg gcgcgcggcg aggccgaggt
       61 cctcgccatg gacttcgcct tcctcctcga ccatgacaga cagctcctgc acatcggcta
      121 caacgtggat accgagcagc tcgacgccaa ccactacgac ctgctggcct ccgaagcgcg
      181 cctcgcgagc ctggtggccg tcgccaaggg cgacgcgccc gtcagccact gggtccacct
      241 gaaccggccg ctcggcgacc tgggcggtcg ggccgcgctc ctctcgtgga gcgggacgat
      301 gttcgagtac ctgatgccgg aactgctcac gcggcgctac ggcgcgacgc tcctcgacca
      361 gagctgcgca gccgcgatcg acgtgcagat cgcgtacgcc aagtgtaacg gcgtgccgtg
      421 gggcgtgagt gagtccggct acgcccgcgt cgatgcggcg gggaactacc agtaccacgc
      481 gttcggcgtg ccggaactgg cgttgaaccg ctcctactcg aacgatctcg tcatcgcgcc
      541 ttacgccagc gtgctcgccc tcaggcaccg gccgcgcgcg gtgctcgaga acgccgcgcg
      601 cctcgccgag gcgggggcgc tcgggccgca tgggttcttc gaggcgctcg actacacgcc
      661 ggagcgccga ccgctagggg aggagcgagt cgtcgtgcgc tctcacatgg cgcaccacca
      721 agggatgaca ctgctcgccc ttaccaacgc tctgacgggc gacgccatgg tggagcgttt
      781 ccacgccgac ccgcgcgtcg cgacgatcga gtacctcctg caggagcgca tcccgctcga
      841 cgtgcccgtg gtggacgacc tgcgtccgga agccagtccg agcgcgccca tcgacgtggc
      901 cgtcctggac gagccgtggg acgtgccggt cgacaccgcc acaccgctga cgcacctctt
      961 gtccaacggc gcatacacgg tcatggtgag cgaggccggt ggcggctggt ccgccgcggg
     1021 cgaagtggcc atcacgaggt ggcgcccgga cgggacgcgc gatgcggacg gcacgtggct
     1081 cttcatccac gacgagggcg aaggcttgac gtggtccccg acgcgcgcac cgttgccggc
     1141 gcgcgcgcag ggcgaccggg tcgagttcta cccttacaag gcgcggtacc agcgcgaggt
     1201 aggcggcatc ctggcccgcc tggaggtgac ggtcgccaag gggacggccg aactgcggcg
     1261 cctgacggtc gtcaaccgcg gcgagacgag caggaccctg cgcgtgacgt cctacgcgga
     1321 ggtcgtcctc ggtccggtcg ccgcggacgc gcgtcacccc gccttcagca agctcttcgt
     1381 cgagagcgcc ttcctgccgc gcaggtccgc catcctgttc cggcgccggc cgcgctccgc
     1441 cgacgagccg gcgctcttcg cggcccatgc cgtcgtctgc aaggacgacc tcggcccgtg
     1501 cgagtacgag acggaccgcg cggcgttcct cgggcgcggc aacgacgtcg ccgcggccgt
     1561 cggaccgcgc ggcccgctgc ggacgggagg cggtccgcgc cgtgcggacg accgtcgcgc
     1621 gggggagaag agcgcgaccc tcgacccggc gatggcgctg gccgtgcggt tcgatctgag
     1681 accgcactcg caggaagagc tgacgttcgt cacggccagc ggcgccagca gccgggacct
     1741 gacgaccctc ctcgacgagc ttcgcaagcc gggcatggtg gaccgcgagt tcgaccttgc
     1801 caggctccgt agccgcgtcg agctgcaaga gcacgacctg acgccgagcg cggtcgagac
     1861 gtaccaacgc ctctattcgg ccgtcaccta ccccgtccag gagctacggg ccccggcggc
     1921 gacgctggcg gccagcacgt tgggccagcc ctcgctgtgg gggcagggca tctccggcga
     1981 cctgccgatc gtgctggtga gcgtcggctc agccgaacgg ttgcccctgg tcgacgatgt
     2041 cctgcgcgcc cacgcctact ggcgccgtcg cggcgtgaag gtggacgtcg tgctcctcaa
     2101 cctgcaggac accgcgtacg accaggcgct gcatgcgcgc ctccgcagcc tgctcgtcag
     2161 gtcgggcagc gacgcatggc tggcggtgaa cggtggcgtc catctcctca ggcgcgacca
     2221 gctcgccccg ctcgacctcg tgttgttgga gagcgccgct gccgtggtcc tggacggcga
     2281 gcacggctcc ctcgccgagc agttgcgaga gctcggccga ccgccccgac ccggcctccc
     2341 gctcctcgtc caggggcttg agcccatgca gggtccgtcg gaaggccccg ctggcaccgt
     2401 gaggctcagg tacgacaacg gctggggcgg gttcacggag gacgggcgcg agtacgtcat
     2461 ccgcgcacgt aggggttcgc tccccccggc gccgtggtcg aacgtcatcg cgaacgcgga
     2521 actcggttgc ctcgtgacgg aggccggcat gggcgccacg tgggcggtga acagcggcga
     2581 gaaccgcctc acgccctgga gcaacgaccc cgtcaccgac cccggttccg agatcgtcta
     2641 cctgcgcgac gaggagacga cggaagtctg gtcggcgacg ccccagcccg cggggggtga
     2701 ggcgtcgttc gaggtgcgcc acggcaccgg tcgcacgctc taccgccacg tgggccacgg
     2761 cctcgaacac gcgctcgagg tatccgtcgc ggcgcgcgac cccgtcaagc tcgtgcgcct
     2821 gcacgtcagg aacctctcgg gcagaccgcg gcgcgtgacg gtcacctact acgccgaatg
     2881 ggtgctcggc gtcgaccgcc gcggaacggg cctccacgtg gtgcccgact acgccccgga
     2941 gcggtgcacg ctcctcgccc gcaaccccta ttccaccgac ttcggtgcgc gcgtcgcctt
     3001 cctcgccggc aacaagagcc cgcacggcgt cacgactgac cgacgggagt tcatcggtcg
     3061 cctaggctcg tacgccctac ccgacgcgct gcggcgcgtc ggccttgccg gcgcggtcgc
     3121 tccgggagcc gacccgtgcg cggccatgca gctgcacctc gacctcgccg ccggcgcatc
     3181 cgaggacgtg ctcttcgcgc tcggccaggc cgacgaccgt gacgccgcgc tgcgactcgc
     3241 cgaccgctac cgcgacctgg gcgcctacgc ggcggccagg agggcggcgg acgaggcatg
     3301 ggatgcggtg ctcggcaagg tgcaagtcag cacccccgac cctgggctcg acctcctgac
     3361 gaaccggtgg ctcctctacc agacgctctc cagccgctgc ttcggccgca cggccttcta
     3421 ccagcccggc ggggcgtacg gcttccggga ccagctccag gacgtcgtgg cgctcctgca
     3481 cgcggctccg gagctcgcgc gtgagcagct gctccgcgcg gcggcccacc agttcccgga
     3541 gggcgacgtg ctgcactggt ggcacccccc cgccggccgc ggcgtgcgca cccacatcag
     3601 cgacgacctg ctgtggctcg tctacaccac cgaacggtac gtctctgcca cgggggaccg
     3661 ctcggtgctg gacgaggagg ccccgttcct cgaggggcgc ccgttggcgc cggacgtgga
     3721 ggatcgctac gacgcctacc gggccgggtc agagtcaggc acgctgctcg agcactgccg
     3781 cagggcgctg cggaagggcg cgacggcggg cagccacggg ctgccgctca tgggcacggg
     3841 tgactggaac gatggcatga accgcgtcgg ggccgacggc aggggcgaat cggtgtggct
     3901 ggcatggttc ctcatcgacg tcatcgggcg gttccgccgg ctcctcgaca gcgtgggcgg
     3961 ccacgaggag gaggcggcgg gctgggcggc gcgagctggc gcctacgctc atgcttgcga
     4021 ggaggcgggt tgggacggtg gttggtacct gcgcgccttc tacgacgacg gcacgcccct
     4081 cggcacggct gccgcgtccg aatggcgcat cgactcgata gcccagtcgt gggccgtgct
     4141 ctccggggcc gcgcccgccg agcgcgccgc aacggccatg gcggcagtcg aggagctctg
     4201 cgtgagacgg caggacggcc tgatcaggtt ggccacgccg ccgttcgacc gctctaagaa
     4261 agacccgggc tacatcaagg gttacgtgcc cggcgtgcgc gagaacggcg ggcagtacac
     4321 gcatggggcc atctggagcg cctgggcgtt cgcgcgtctc ggcgagcgcg gcaaggccca
     4381 cgcactgcac cggctcctcg acccggtcct ccgcgccggc gatcgcgagg gcgccgaacg
     4441 ttacgtggtg gagccgtacg tgatggcggc cgacgtctac gagcgacccc cgcacgtcgg
     4501 gcgcgggggc tggacctggt acacgggctc cgccgcctgg ttctaccgtt tcggtctcga
     4561 ggggcttctc ggtcttgaac gcgtgggaga cgagctgcga ctaacgccgc gcgtgccgac
     4621 cgagtggcct ggcttcgagg tgcgttaccg ccacgggtcg agcacctacg tcctgcgtgc
     4681 caggccgggc gcgatccccg ccaaccaggt cgtgaagctc agggacgacg gcggggagca
     4741 cgtggtggac gtggtgtacg aggcgtaggt cgggctggcc gcccgaccgt ccgtggtcca
     4801 cgagcgcgcc gagtccgtac tggcccgggg gcgatcagcc ggtaccagga cgcgtggcct
     4861 gtgagcgcgc cgagtaagct cggcccgcag gccgcaggac ctcagagccc ggagaggcgc
     4921 gtgttcttct cgatgtagtc agcccactcg tccggcacgt ccatctccgg gtagatggcg
     4981 tcgacggggc aggccggaac gcaggcaccg cagtcgatgc actcctcagg gttgatgtag
     5041 aactggtcgt ccgcctcgta gatgcactcc accgggcaga cgtctacgca cgaggcgtct
     5101 ttcacgccga tgcagggttc cgtgatgatg taggccattg tcgccatcct tgtcacccga
     5161 gcgccgttga tgcgggtacg gcgttcgggc cgcgacgctt ccgcgtcttc gctgaggcag
     5221 ttctagcagg tgcggcccga ccgtggcaag aactttcgta cctaatgcga cgagcatggc
     5281 acagcgagcg gccccggctg cctttgttcg ctctgtcggt gcccgcctcc ggtggttcat
     5341 cgtcctgcta cttgccggtc ttgccgtggt gccttactta cgggagccgg gttactcggt
     5401 cgagcctcta gtggcgcagg ggccgctctt caagaagtgg tccgtttgtt ctatggtatc
     5461 cagacctcct tccgactcaa aggtactccc gtcggccgct gatgaacagg gccacacgtc
     5521 atggaaaacg cgtggccctt tagctaaact aaatgtttgg ggccgcgcga tggcggcacc
     5581 ggagaggccg ccggaagcgg caccacccct acctcagaac aggaaccaac cgcccgccac
     5641 cgcgatcacg cccgcgaaga tgagcgcgac ggtgttgatg accttgatga gcgggttgat
     5701 ggacgggcca gccgtgtcct tgtaagggtc gcccaccgtg tcgccgacca cgctcgccgc
     5761 gtgggcgtcg gagcccttgc cgccgtggtt gccgtcctcg atgtacttct tggcgttgtc
     5821 ccaggcgc
//
LOCUS       DAOQYE010000018         5820 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_14064_length_5820_cov_2.665221, whole genome shotgun sequence.
ACCESSION   DAOQYE010000018 DAOQYE010000000
VERSION     DAOQYE010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 5820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5820
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14064_length_5820_cov_2.665221"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..670
                     /locus_tag="PKN52_00770"
     CDS             <1..670
                     /locus_tag="PKN52_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-glucan phosphorylase"
                     /protein_id="HNQ98496.1"
                     /translation="GTNGWAIGTGRTYQDDARSDAADADALYDLLVREVVPLYYRRDA
                     DGVPEAWMRRAAKAIATISPRFNAQRMVREYVESYYAPASERGATLSADGYRKATELA
                     DWLKKVRDEWHNVRFTASPLTEAPLRIGDEVELSAELNLGSLAPEDVIVEVVYSREGD
                     GLEERLERVAMRARGDGTQDGTQAYTARFKPKLSGRLVYGARVVATNPLLSSPFDAHA
                     IKWA"
     gene            complement(657..1424)
                     /gene="pgl"
                     /locus_tag="PKN52_00775"
     CDS             complement(657..1424)
                     /gene="pgl"
                     /locus_tag="PKN52_00775"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HNQ98497.1"
                     /translation="MSAEIIRTPDVTATALPLVVDGLRSAVALRGRASLVLAGGSTPL
                     PLYRELAGVDLPWERVHFVWGDERRVSVTDSRSNARSALEALIDGLDVPEEHVHLWPR
                     LPSGEAAAAAYQGELEAALGPEPVFDVTILGLGDDGHTASLFPHTGAALSGAFALATT
                     APSGVVVAERLSLGARTLSRSRLVLFLVQGATKAAAFAATFGPTAPDVAVRPPWTVEV
                     SAELASVVDAHPALAISALGRLVVVTDLDLPRTLKPT"
     gene            1482..2066
                     /locus_tag="PKN52_00780"
     CDS             1482..2066
                     /locus_tag="PKN52_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HNQ98498.1"
                     /translation="MEPTREEALRLMEEWTPSESLRKHMLAVEAAVTAYADKYGEPRG
                     PWAVAALLHDFDYERYPKVGVDGHPFVGVRELESRGYPRLILDAILGHAGEVTGVKRE
                     TLLAKALFACDELTGLITAAVLVRPDKDIAGLGLSSLKKKLKDKAFARGVNRDDVRQG
                     ATELGIDLDEHMQFVLSAMQARAAELGLDGSLAG"
     gene            complement(2087..2686)
                     /gene="lepB"
                     /locus_tag="PKN52_00785"
     CDS             complement(2087..2686)
                     /gene="lepB"
                     /locus_tag="PKN52_00785"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNQ98499.1"
                     /translation="MRGRYLLAVGLGLLLGIALTQWGATLMRVHGASMEPTLEDGEWV
                     LVVREPLAHLLGGMGSRAEPGTVVVVADPAPARAGSAAARLPSPLAGLFAKLLVKRVA
                     AVGGQVVALVDGKVEVDGAVSPEPWLDGVPSAHVSYPAVRVPPGTVFLLGDNRLPLAS
                     RDSRQFGPLGTTSLRGRVVAHVRWPVARGAWRWPVAQVR"
     gene            complement(2683..3693)
                     /locus_tag="PKN52_00790"
     CDS             complement(2683..3693)
                     /locus_tag="PKN52_00790"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNQ98500.1"
                     /translation="MERAGRHICVGLVLSGGGARGFAHIGVLRVLERAGAKFDVVAGT
                     SMGAILGSLYASGLSADELYELATATDWTDVVDLSLKSGLLKGDRLAGFLAERLPATF
                     EELKLPFATVATDIESGEEVVMTEGDLVSAVRASAAFPGAFEPVHLAGRTLADGGIIN
                     NLPINAASWLGANRVIASDVTPPRQSVYTTLEQEGSWWERMVATVKLERRNPMAQMLF
                     RASDIQQSILVDINATIHPADLRIRIPMPQHRIESFMNFEEIVWEGERAAELALAGAG
                     GWKSVLAETGPVAEEARGAQGAQGVAGAPVAAQETGELGVAAVAKTTLARVIGRGGKK
                     RA"
     gene            complement(3827..5113)
                     /locus_tag="PKN52_00795"
     CDS             complement(3827..5113)
                     /locus_tag="PKN52_00795"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNQ98501.1"
                     /translation="MLRNRSLSVFGTLLLLLAVAAVWLPGSPVAAQPSAVQERSALLQ
                     DEQNTVDIVEEWGPSVVAINVEVRGSRLGQDPLEGLPPQFRNLLPQQLIPQQPQVQQS
                     SGSGFVVGENEIVTNYHVVENALVSNGVAKVEGATIRVVFPDVDDEYDAEVVGADPDY
                     DLALLRLVDGGKMPAAAKPLELADSTAVRVGQKVIAIGNPFGLQSSVSQGIVSAIGRE
                     LPSIGQIEIPMVQTDAAINPGNSGGPLLDSAGRLVGVNTMIVPGMTSSGSAGNIGIGF
                     AVPSELLIEALPLMREGGLVGVYAQNLDIVNRPRIGIEVGAVTAIPDEARRVLRYPDH
                     GLVVVSVAPGGPAQAAGLEGPAFQATIGNSSYPAGGDVILSANGTKIDQAADLQKLLI
                     GAKAGDEMTLEVWRNGETRTVKVTLQVVSQPEESGN"
     gene            complement(5190..>5820)
                     /locus_tag="PKN52_00800"
     CDS             complement(5190..>5820)
                     /locus_tag="PKN52_00800"
                     /inference="COORDINATES: protein motif:HMM:NF015951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HNQ98502.1"
                     /translation="MAFFMGLVAASTAIVGRNVKRWNAPVALLLALGSAAGFVIVGLT
                     PVETPSTPAFLFLSGFLAICAMVLPGLSGAFILVLLEKYEFALAALGNLDLAVIVPIL
                     LGAAVGLLSFARFLTYLLKRFHDATLALLTGFMLGSLRKVWPFVDGTGAATWPWATGD
                     VGAAILMSALILFGLLIVLVIDRLAKAASRSGSVSRTSFSSTRRFTPRR"
BASE COUNT          928 a         2006 c         2076 g          810 t
ORIGIN      
        1 cggcaccaac ggctgggcca tcggcacggg ccggacttac caggacgacg cgcgctccga
       61 cgctgcggac gccgacgccc tctacgacct cctcgtgcgc gaggtcgtcc cgctgtacta
      121 ccgacgcgac gccgacgggg tgcccgaggc ctggatgcgc agggccgcca aggcgatcgc
      181 gaccatctcg ccgcgcttca acgcccagcg catggtgcgt gagtacgtcg agagctacta
      241 cgcgccggcc agcgagcgcg gcgccacgct gagcgccgac gggtaccgca aggccaccga
      301 actcgccgac tggctcaaga aagtgcgcga cgagtggcac aacgtgcggt tcacggccag
      361 cccgctcacc gaggcgccgc tcaggatcgg cgacgaggtg gaactgagcg ctgaactgaa
      421 cctcggctcc ctcgcgccgg aggacgtgat cgtcgaggtc gtgtacagcc gcgaaggcga
      481 cggcctggag gagcgtctcg agcgcgtcgc gatgcgggcg cggggcgacg gcacccagga
      541 cggcacccag gcctacacgg cgcgcttcaa acccaagctg tcgggcaggc tcgtctacgg
      601 cgcgcgcgtc gtggctacga acccgctgct gagctcaccg ttcgatgcgc acgcgatcaa
      661 gtgggcttga gcgttcgcgg caggtccaga tccgtcacga cgacgagccg cccaagggcc
      721 gagatggcca gcgccgggtg ggcgtcgacc accgaggcca gctccgccga gacctcaacc
      781 gtccacggcg gacggacggc cacgtccggc gctgtcggtc cgaacgtcgc cgcgaaggcc
      841 gccgccttgg tagcgccctg cacgaggaac agcacgagtc ggctgcggct cagcgtcctg
      901 gcgcctaggg agaggcgctc ggcgaccacc acgccgctcg gcgcggtggt ggccaaggcg
      961 aaggccccgg agagcgccgc gcccgtatgg gggaagaggc tggccgtgtg cccgtcgtcg
     1021 ccgaggccga ggatggtcac gtcgaacacg ggctcggggc cgagggccgc ctcgagctcg
     1081 ccctggtagg ccgcggcggc ggcctcgccg ctaggcagtc gcggccagag gtgcacgtgc
     1141 tcctcgggca cgtcaaggcc gtcgatcagc gcctccaacg ccgagcgggc gttgctgcgc
     1201 gagtcggtga cgctcacgcg gcgctcgtcg ccccagacga agtggacgcg ctcccacggc
     1261 aggtcgaccc ccgcgagttc ccggtagagg ggcagtggag tggagccgcc cgccagcacg
     1321 aggctggcgc gcccacgcag cgccacggcg ctccgcaggc cgtccaccac cagcggcagg
     1381 gccgttgccg tcacgtcggg cgtgcgaatg atctcggctg acatgccgga gtatagccag
     1441 gcacctcggg caccggtccg gcggtcgcgt taaactcccg catggagccg acacgtgaag
     1501 aggcgctgcg cctcatggaa gagtggacgc cttcggagag cttgcgcaag cacatgctgg
     1561 ccgtggaagc cgcggtgacc gcgtacgccg acaagtacgg cgagccgcgg gggccatggg
     1621 cggtggccgc cctgctgcac gacttcgatt acgagcgcta tccgaaagtc ggggtggatg
     1681 ggcatccgtt cgtcggcgta cgggaactcg agtcgagggg ctacccgcgc ctgatcctgg
     1741 atgcgatcct cggccacgcc ggcgaggtca cgggcgtgaa gcgcgagacg ctgctggcca
     1801 aggcgctgtt cgcctgcgac gagctgacgg gtctcatcac cgccgccgtc ctcgtgcggc
     1861 cggacaagga catcgctggt ctcgggctct cgagcctgaa gaagaagctg aaggacaagg
     1921 ctttcgcgcg gggcgtcaac cgcgacgacg tgcgccaggg cgccacagag ctgggcatcg
     1981 atctcgacga gcacatgcag ttcgtgctga gcgccatgca ggcgcgcgcg gcggaactag
     2041 ggctcgacgg ctcgctggcc ggctgagccc cggctccgcg gtaccctcac cgcacctgcg
     2101 ccaccggcca ccgccacgct ccacgcgcca ccggccacct cacgtgggcg accacgcggc
     2161 cgcgtaggga ggtcgtgccc agggggccga actgcctgga gtcgcgactg gccagcggca
     2221 gccggttgtc gccgagcagg aagaccgttc ccgggggcac ccggacggcc gggtaggaga
     2281 catgggcgct cggcacgccg tcgagccacg gctccggcga caccgcgccg tccacctcca
     2341 ccttcccgtc gaccaacgcc acgacttggc cgccgacggc cgccacgcgc ttgacgagca
     2401 gcttcgcgaa gagcccggcc aagggggagg ggagtcgcgc cgcggcggaa ccggcgcgag
     2461 ccggcgcggg gtcggcgacg accaccacgg tgcccggctc cgctcgactt cccatgccgc
     2521 cgagcaagtg cgcgaggggc tcgcggacca cgaggaccca ctccccgtct tcgagggtgg
     2581 gttccatgct cgcgccgtgc acgcgcatga gcgtcgcgcc ccactgagtc aaggctatcc
     2641 ccaggaggag gcccagcccg accgcgagca ggtagcggcc tctcaagcgc gcttcttgcc
     2701 gcccctaccg atcacccttg ccagggtggt cttggcgacg gcagccacgc cgagctcgcc
     2761 cgtttcctgg gcggcgaccg gcgcgcccgc cacgccctgc gcaccttgcg cgccccgcgc
     2821 ctcctctgca accggaccag tctcggccaa cacgctcttc caaccgccgg cgccggccag
     2881 cgcgagctcc gccgcccgct cgccttccca cacgatctcc tcgaagttca tgaacgactc
     2941 gatccggtgc tgcggcatgg ggatgcggat gcgcaggtcc gcggggtgga tggtcgcgtt
     3001 gatgtcgacg agtatcgact gttggatgtc agacgcgcgg aagagcatct gcgccatcgg
     3061 gttgcgccgc tccagcttga ccgtcgccac catccgctcc caccaactcc cctcctgttc
     3121 cagggtcgtg tacacggact ggcgcggcgg cgtgacgtcg gaagcgatga cgcggttggc
     3181 gccaagccaa ctggcggcgt tgatgggcag gttgttgatg atgcccccgt cggcgagcgt
     3241 gcggcccgcc agatgaaccg gctcgaacgc gcccgggaag gcggccgagg cgcgcacggc
     3301 gctgacgagg tcgccctccg tcatgacgac ctcctcaccg gactcgatgt ccgtagcgac
     3361 cgtcgcgaag ggcagcttca actcctcgaa ggtggccggc aggcgctcgg cgaggaaccc
     3421 cgcgaggcga tcgcccttga gcaggccgct cttgagggag aggtcgacca catcggtcca
     3481 gtcggtggcg gtggccagtt cgtatagctc gtcggcggag aggccgctcg cgtacaggga
     3541 gccgaggatg gcgcccatcg acgtgcccgc caccacgtcg aacttggcgc cggcgcgttc
     3601 cagtacccgc agcaccccga tatgcgcgaa gccgcgcgcc ccgccgcccg atagaacgag
     3661 cccaacacag atgtgacgtc cagcacgctc catgtcgcga tcctagcacg ggcgttatga
     3721 gccacagccc tcggttgaag cacgtcccgc ggtcgggttc gagacgcggg acgtgtatgt
     3781 aggacgttgg acccggggag ggccctccga gtgctgccga cggacctcag ttgccgcttt
     3841 cttccggctg cgagacgacc tggagggtca ccttgaccgt gcgcgtctca ccgttgcgcc
     3901 agacctcgag cgtcatctcg tcaccggcct tggcgccgat gagcagcttc tgcaagtcgg
     3961 ccgcctggtc gatcttggtg ccgttggccg agaggatcac gtcgccgccg gcggggtagc
     4021 tgctgttgcc gatggtggct tggaaggccg ggccctcgag tccggccgcc tgggccggtc
     4081 cgccgggcgc gacgctgacg acgacgaggc cgtggtccgg ataacggagc accctgcgcg
     4141 cctcgtcggg gatggccgtg acggcgccga cctcgatgcc gatgcgcgga cggttgacga
     4201 tgtccaggtt ctgggcgtac acgccgacga ggccgccctc gcgcatgagc gggagcgcct
     4261 cgatcaggag ctcgctcggc accgcgaagc cgatgccgat gttcccggcg ctgccgctgc
     4321 tcgtcatgcc cggcacgatc atcgtgttca cgccgacgag ccgacccgcc gagtcgagga
     4381 gcgggccccc agagttaccg gggttgatgg cggcgtcggt ctggaccatc gggatctcga
     4441 tctggccgat ggaggggagc tcgcggccga tggccgacac gatgccctgc gagacgctcg
     4501 actggaggcc gaacgggttg ccgatggcga tgaccttctg accaacgcgc acggccgtgc
     4561 tgtccgccaa ctcgaggggc ttggctgcgg cgggcatctt gccgccgtcc acgagccgca
     4621 ggagcgcgag gtcgtagtcc gggtcggcgc cgacgacctc ggcatcgtac tcgtcgtcta
     4681 cgtcggggaa cacgaccctg atggtcgcgc cctcgacctt cgcgaccccg ttactcacca
     4741 gcgcgttctc gacgacgtgg tagttggtga cgatctcgtt ctcacctacc acgaagccgg
     4801 agccgctgct ctgctgcact tgcggctgtt gcgggatcag ctgctggggg aggaggttcc
     4861 tgaactgcgg tggcaggccc tcgagggggt cctggccgag gcggctgccg cgcacctcga
     4921 cgttgatggc cacgacgctc ggcccccact cctcgacgat gtcgacggtg ttctgctcgt
     4981 cctggaggag cgccgatcgc tcctgcaccg cgctcggctg cgccgcgacg gggctgcctg
     5041 gcaaccagac ggccgccacg gccagcagca ggagtagtgt cccgaagacg ctgagtgaac
     5101 ggttcctgag cacttggatc ctccaatttg gctctcatcg actgggagcc ggtaggaggc
     5161 gcgtgggcgc gcccctgacg tgctggaggc taacgccgtg gtgtgaagcg acgcgtagat
     5221 gagaatgagg ttcgtgaaac ggagccggac cggctcgcgg ccttggcgag cctgtcaatg
     5281 acgagcacga tgagcagacc gaacaagatg agcgcactca tgaggatcgc ggctccgacg
     5341 tcgccggtag cccacggcca ggtcgcggcc cccgtgccgt ccacgaacgg ccagaccttg
     5401 cgcaggctcc cgagcatgaa acccgtgagc agggccaggg tggcgtcgtg gaagcgtttg
     5461 agcaggtagg tcaggaagcg cgcgaaggag aggaggccga cggcggcgcc gagcaagatc
     5521 gggacgatca cggcgaggtc caggttgccg agcgcggcga gggcgaactc gtacttctcg
     5581 agcagtacca ggatgaacgc gccggagagc ccgggcagca ccatggcgca gatggcgagg
     5641 aagcccgaca ggaagaggaa agcgggcgtc gatggggtct ccacgggcgt gaggccgacg
     5701 atgacgaagc cggcggcgct cccgagcgcc agcagcagtg cgactggcgc gttccaccgc
     5761 ttcacgttgc ggccgacgat ggcggtcgag gcggccacga ggcccatgaa gaacgccatc
//
LOCUS       DAOQYE010000019         5785 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_14177_length_5785_cov_2.936300, whole genome shotgun sequence.
ACCESSION   DAOQYE010000019 DAOQYE010000000
VERSION     DAOQYE010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 5785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5785
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14177_length_5785_cov_2.936300"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="PKN52_00805"
     CDS             <1..200
                     /locus_tag="PKN52_00805"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNQ98503.1"
                     /translation="DDYALTFAGMFPIEKPKVVVVVMLMKPEGDSTSTYVAAPIFRAI
                     GSEVVAHWGVAPQADAVAQVR"
     gene            295..1704
                     /locus_tag="PKN52_00810"
     CDS             295..1704
                     /locus_tag="PKN52_00810"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HNQ98504.1"
                     /translation="MTLLSARSLVRLLNTVEPVEDLPDATGIAFDSRRVRPGDAFFAL
                     PGESGHGIAFAEAALARGAAFVVSDRPHRRGLLVDDARAAILALGRAARDRLKGQVIG
                     VSGSNGKTSTKALLAAALPGRSTPGNLNTHYAIAGALVDAAMQDLGALDGAEGTPLVL
                     ELGIDRPGEMAELLELTRPDHALLTTVAASHTQALGDVAGVAREKTLLLQRTPGVKVA
                     GATAAEHLDAATLADTVVVAVASSRAPLPSSFAGAAATVVGSAELLPEGMTVLRWQDV
                     QGGGTVVLPWPGRAMAQNALAAVTLAGLLGSPQATAWERAAQAKLEHGRLERSTLDGL
                     TLLDDSYNSNPASLAEALAVLRACPAPRAAFLGDMRELGSLDRESHLRAGEATRGLEL
                     VVAVGPSSAALLQTNPLAVHAEDAASACAFLDRVPRGATLLVKGSRSVGMELVVEAAR
                     VRAAAGAWRAPSRSALESA"
     gene            1701..2621
                     /locus_tag="PKN52_00815"
     CDS             1701..2621
                     /locus_tag="PKN52_00815"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98505.1"
                     /translation="MMLTTVVAAAISLVLTGVFVQLAQRLGWGKAIRAQGPESHLVKA
                     GTPTMAGVAFLLAAGIAWLLFAGDELKPELALVLLTLGSGLLGLYDDSLSLTRKRSRA
                     LGLEEQSTGLMARYRLLGQGALALAFGWWAARGGAVLFGIGWLDVLAFAFVIVGSINA
                     FNFTDGIDSLAAGVTAVVLLFFLASPFAAALLGALLGFLWYNSQPARTFMGGVGSEAL
                     GAAVAGLAILQGSVWYLPLVAAIPVLEVLSVVAQVAYFKATGGKRLLRMSPLHHHFEL
                     GGWSGPRIATRFQLVTALCVALAFAIRQAS"
     gene            2622..4001
                     /gene="murD"
                     /locus_tag="PKN52_00820"
     CDS             2622..4001
                     /gene="murD"
                     /locus_tag="PKN52_00820"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HNQ98506.1"
                     /translation="MSGEVLVYGLGRSGLAVVRRLRESGAPAAFFDRRGSGPDVAEAL
                     ALGARSVAEVRSDRFALAIAAPGVPIDHPDLAALRAAGTEVIGEVEWVWRTTPGTYVG
                     ISGTAGKGSVTRWTADTLAAAGMDAVAGGNIVPALAAVAKPDRVHVVEMSSFQLERCP
                     TFAPDVAVLLNLGEDHIDRHGSVAAYHAAKRNLIANLGPDSTFVVNADDPVLSRWAAE
                     SEAAGVDVKRFSLATPAAAYRTPAGMLVLEGEPLLPSSDLHVLGEHQVANALAVALAC
                     AALGAPTTAIAAGLGAFTGLPGRYSVAGHFGGVRFLEDSIATRPLSVAAALRSTPRPL
                     VWLAGGQGKGASVAELRELVAEKVDLLVLIGASADEFRSAFGDLVPTLLIEASDGRAA
                     MRAAVGAAHAHLQERHGGAGNVLLAPLAASFDQFEDYADRAAAFREAVAAVRRASGSA
                     GAVAAGADA"
     gene            4001..5110
                     /locus_tag="PKN52_00825"
     CDS             4001..5110
                     /locus_tag="PKN52_00825"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HNQ98507.1"
                     /translation="MDRLLLSIQVTLGLLGVVGVAAAAPDTALEHGLRFAFALLLTLA
                     FSRLREKHVLKLSPYAFVTLLFLLVLVLVIGVSPAGSESKRWLLVGGISLQPSELMKV
                     AVIAYLAAFFHNHLGNWEIWRPMLVIGLTAGLIVIEPDVSTAAFVFLLAVAIMIAAGA
                     TLTRVLAIMFTAAVTAALLAGTVLSQFTYIGERMAGYFDRWGQQSQASDLSYQALRAL
                     EAIGRGGVLGIGAGRRVPVPEAETDFVGVAIAHSLGFLGAVTLVAMYALLAWHGYRIA
                     RRVTGPSALLAAGATAYICGQAGLNLLVVTGMFPVTGLPLPGVSYGLNSQVSVAIAFG
                     FLHMASKLAETRPAAEATAPVTARFVSAPTGRQRA"
     gene            5107..>5785
                     /locus_tag="PKN52_00830"
     CDS             5107..>5785
                     /locus_tag="PKN52_00830"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HNQ98508.1"
                     /translation="MSAARRLLLATGGTGGHIYPALAVAEEAAARGVEVAVVGGLGAM
                     EERLCQEAGVQFFGVDTGKWDRQRPDPRQALRAASGLRQAVNVVGRLRPDAVVGFGGF
                     ASMPGCFAAWRLRVPLVLHEGNAFPSRVNRWFARAARLFITAQPEALAHVRARATLVV
                     PFPVRETRMERGAARTALGLPDSTLVTLVMGGSQGSLALNRAVPEAYEKLAGSGGADA
                     LVVVHSTG"
BASE COUNT          737 a         2197 c         1976 g          875 t
ORIGIN      
        1 cggacgacta cgccctgacc ttcgcgggga tgttccccat cgagaagccg aaggtcgtcg
       61 tcgtcgtgat gctcatgaag cccgaaggcg acagcacgag cacctacgtg gccgccccca
      121 tcttccgggc gataggcagc gaggtcgtgg cgcactgggg cgtggcgccc caggcggacg
      181 cggtggcgca ggtcagatga cccgctcgcg tcgctgagcg gccggcagcg ccatcagacc
      241 tatgcggcat agcagtatgt tgtgtaagat agtcaacatg cggaggttgg tgtcatgacg
      301 ctcctgagcg cgcgctcgct ggtgaggctc ttgaacaccg tggagccggt ggaggacctg
      361 cccgacgcca ccggcatcgc gttcgacagc cgccgcgttc gccccggcga cgccttcttc
      421 gcgctccccg gcgagagcgg tcacggcatc gccttcgccg aggccgccct cgcgcgcggc
      481 gcggcgttcg tggtgtccga tcggccccac cgtcgcggcc tgctggtcga cgacgccagg
      541 gccgccatcc tcgccctcgg gcgcgccgcc agggaccgcc ttaagggcca agtcatcggg
      601 gtcagcggct cgaacggcaa gacgagcacg aaagccctgc tggccgctgc gctgccggga
      661 cggtcgacgc ctggcaacct caacacccac tacgccatcg ccggcgccct cgtcgacgcc
      721 gccatgcagg acctcggggc gctggacggc gccgagggca ctccgctcgt cttggagctc
      781 ggcatcgaca ggccgggcga gatggcggag ctgctggaac tgacccgccc ggaccacgcg
      841 ctcctcacaa ccgtggccgc cagccacacc caggcgctcg gcgacgtggc cggcgtcgcc
      901 cgcgagaaga ccctcctcct ccagcgcacg cctggcgtga aggtcgccgg ggcgacggcc
      961 gccgagcatc tcgacgccgc cacgctggcg gacacggtgg tagtggcggt ggccagcagc
     1021 cgagctccgc tgccgtcaag cttcgccggg gcagccgcca ccgtggtcgg cagcgccgag
     1081 ctcctgcccg agggcatgac ggtgctccgc tggcaagacg tgcaaggcgg aggcaccgtc
     1141 gtcctcccct ggccgggcag ggccatggct cagaacgccc tcgccgccgt cacgctggcc
     1201 ggcttgctcg gcagtccgca ggcgaccgcg tgggagcgcg cagcgcaggc gaagctcgaa
     1261 cacggccggc tcgagcgcag cacgctcgac ggcctcacgc tcctggacga cagttacaac
     1321 tccaacccgg cctcgctcgc cgaggccttg gcagttctgc gcgcctgccc ggccccgcgc
     1381 gcggcgttcc tcggcgacat gcgcgaactc gggagcctcg acagggagag ccacctgcgc
     1441 gccggcgagg cgacgcgggg cctcgagttg gtagtcgccg tcggaccgtc gagcgccgcg
     1501 ctgctccaga cgaacccgct ggccgtccat gcggaggacg ccgccagcgc gtgcgcgttc
     1561 ctcgacaggg tcccgcgcgg cgccactctg ctcgtcaagg gctcgcggag cgtcgggatg
     1621 gagctggtcg tcgaggccgc gcgcgtgcgc gccgcggccg gagcttggcg cgcgccgagc
     1681 cgaagcgcct tggagagtgc atgatgctga ccaccgtagt agccgccgcc atcagcctcg
     1741 tgttgacagg ggtcttcgtg cagctcgcgc aacgcctagg ctggggcaag gcgatccgcg
     1801 cccaaggccc cgagtcgcac ctcgtcaagg ccggcacgcc caccatggcg ggcgtggcct
     1861 tcctcctcgc cgccgggatc gcgtggctac tcttcgcggg cgacgaactg aagcccgaac
     1921 tggcgctcgt actcctcacc ctcggcagcg gcctccttgg gctgtacgac gactcgctct
     1981 cgctgacccg caagcgcagc cgcgccctcg gcctcgagga gcagagcacc gggctcatgg
     2041 cccgttaccg cctactcggg caaggcgccc tcgcgctggc gttcggctgg tgggcggcgc
     2101 gcggcggggc cgtcctcttc ggcatcggct ggctcgacgt gctcgccttc gccttcgtga
     2161 tcgtcgggag catcaacgcc ttcaacttca ccgacggcat cgatagtctg gccgccggag
     2221 tcacggccgt cgtgctcctc ttcttcctcg cctccccctt cgccgccgcc ctgctcggcg
     2281 cgctcctcgg cttcctctgg tacaacagcc aaccagcccg gactttcatg ggcggcgtcg
     2341 gctcggaggc gctcggcgcc gccgtggcag gcctcgcgat cctccagggg agcgtctggt
     2401 acctgccgct cgtcgcggcc atccccgtcc tcgaggtcct ctccgtcgtc gctcaggtcg
     2461 cctacttcaa ggcgaccggc ggcaaacgtc tgctgcgcat gagcccccta caccatcact
     2521 tcgagttggg cggctggagc ggcccgcgca tcgcgacgcg tttccaactc gtcaccgccc
     2581 tctgcgtggc tctcgcgttc gccatccgcc aggccagcta gatgagcggc gaggtcctcg
     2641 tctacggcct cggacgctcg gggctcgccg tcgtccgccg gctgcgcgag tcgggcgcgc
     2701 ccgctgcctt cttcgaccgg cgcggaagcg ggcccgacgt cgccgaggcg ctggcactcg
     2761 gcgcccggag cgtcgccgag gtccggagcg accggttcgc gctggccatc gcggcgcccg
     2821 gcgtgcccat cgaccacccg gacctcgccg ccttgcgagc cgccggcacc gaggtcatag
     2881 gcgaggtcga atgggtgtgg cgcacgacgc ctggcaccta cgtcggtatc agcggcacgg
     2941 ccggcaaggg cagcgtgacc cgctggaccg ccgacaccct ggcggcggcc ggcatggacg
     3001 ccgtcgccgg cggcaacatc gtgccagcgt tggccgccgt cgccaagccg gaccgcgtgc
     3061 acgtggtgga gatgtcgtcg ttccagctcg agcgctgccc gacgttcgcc cctgacgtcg
     3121 ccgtgctcct caacctcggc gaagaccaca tcgaccggca cggtagcgtc gccgcctacc
     3181 acgcggccaa gcgcaacctc atcgccaacc tcgggcccga ctcgacgttc gtcgtcaacg
     3241 cggacgaccc ggtcctctcc cgctgggcgg cggagagcga ggccgcgggt gtcgacgtca
     3301 aacgcttctc gctcgcgacc cccgccgccg cgtaccggac gccggcaggc atgctcgtcc
     3361 tcgagggcga gccgttgctg ccgtcgagcg acctgcacgt cctcggcgag caccaagtag
     3421 ccaacgccct ggccgttgcc ctcgcgtgcg ccgccctcgg cgcgccgacc acggccatcg
     3481 ccgccggcct gggcgcgttc accggcctgc ctggccggta ctccgtcgcg ggccacttcg
     3541 gcggcgtacg cttcctcgag gactcgatcg ccacccggcc cctgtcggtc gccgccgccc
     3601 tgcgctcgac gccccggccc ctcgtgtggc tggcaggcgg gcagggcaag ggcgccagcg
     3661 tcgccgagtt gcgcgaactg gtggccgaga aggtggacct cctcgtgctc atcggcgcca
     3721 gcgccgatga gttccgctcg gccttcggcg acctcgtccc gacgctgctg atcgaggcga
     3781 gcgacgggcg tgcggccatg cgtgccgcgg tcggcgccgc ccacgcccac ttgcaggagc
     3841 gccacggcgg ggccggcaac gtcctcctcg cgcccctcgc cgcctcgttc gatcagttcg
     3901 aggactacgc cgacagggcc gccgccttcc gggaggcggt agccgcggtc cgccgcgcct
     3961 ccggctcggc gggagcggtc gcggcgggtg ccgacgcctg atggaccggc tgttactgag
     4021 catccaggtg acgcttgggc tgcttggcgt ggtcggcgtg gccgccgccg cgccggacac
     4081 cgccctcgaa cacggtctac gtttcgcctt cgccctgctc ctcacgctcg cgttctcacg
     4141 tctacgcgag aagcacgtgc tcaagctctc gccgtacgcc ttcgtgaccc ttctcttcct
     4201 cctcgtgctg gtgctggtca tcggcgtgtc gcctgccggc agtgagagca agcggtggct
     4261 cctcgtcggc ggcatcagcc tccaaccgtc ggaactcatg aaggtggccg tgatcgccta
     4321 cctggccgcc ttcttccaca accacctcgg caactgggag atctggcgcc cgatgctcgt
     4381 catcggcctc acggccgggc tcatcgtcat cgaaccggac gtcagcacgg cggccttcgt
     4441 gttcctgctg gccgtcgcga tcatgatcgc ggccggcgcc accctcacgc gtgtcctcgc
     4501 catcatgttc accgccgccg tgacggccgc cctgctggcc gggacggtcc tcagtcagtt
     4561 cacctacatc ggcgagcgga tggcgggcta tttcgaccgc tgggggcagc agtcccaagc
     4621 gtccgacctc tcctaccagg ctttgagggc gctcgaagcc atcggtaggg gcggcgtgct
     4681 cgggatcggg gccggtcgcc gcgtaccggt ccccgaagcc gagacggact tcgtaggcgt
     4741 cgccatagcg cactccctcg gcttcctcgg ggcggtcacg ctcgtggcca tgtacgcgct
     4801 gctggcctgg cacggctacc gcatcgccag gcgcgtcacc gggccgtccg cgctgctcgc
     4861 ggccggagca accgcttaca tctgcgggca ggccggcctc aacctcctcg tcgtcaccgg
     4921 catgttcccc gtgacgggcc tacccctccc cggcgtcagc tacggtctca actcgcaggt
     4981 gtcggtcgcg atcgcgttcg gcttcctgca catggcgagc aagctcgccg agacgcgccc
     5041 tgctgccgag gcgaccgcgc cggtcacggc ccggttcgtg tcggcgccga cggggaggca
     5101 aagagcgtga gcgcggccag gcgcctcctc ctagcgaccg gcggaacggg cgggcacatc
     5161 taccccgccc tcgcggtcgc cgaggaggcc gcggcgcgcg gcgtcgaggt tgccgtcgtg
     5221 ggcgggctcg gcgcgatgga agagcgcctg tgccaggagg ccggcgtcca gttcttcggt
     5281 gtcgacacgg gcaagtggga ccgccagcgc cccgacccgc gacaggcgct cagggcggcc
     5341 tccggcctgc gccaggccgt caacgtcgtg ggccggttgc gacccgacgc cgtggtcggc
     5401 ttcggcggct tcgcttcgat gcccggctgc ttcgcagcct ggcggctgcg agtgcctctc
     5461 gtgctgcacg aaggcaacgc tttcccgagc cgcgtcaacc ggtggttcgc gcgcgcggcc
     5521 aggctcttca taaccgccca gcccgaggcc ctcgcccacg ttcgcgcccg cgccacgctc
     5581 gtagtcccct tccccgtgcg cgagacgcgc atggagcgcg gagcggcgcg cacggcgctc
     5641 ggactgcctg acagcacgct cgtcacgctc gtgatgggcg gctcgcaagg ctcgctggcc
     5701 ctcaaccgtg ccgtgccgga ggcgtacgag aagctcgccg gttccggcgg cgcggacgcg
     5761 ctggtggtcg tccactccac cggac
//
LOCUS       DAOQYE010000020         5783 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_14183_length_5783_cov_3.037011, whole genome shotgun sequence.
ACCESSION   DAOQYE010000020 DAOQYE010000000
VERSION     DAOQYE010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 5783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5783
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14183_length_5783_cov_3.037011"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="PKN52_00835"
     CDS             complement(<1..350)
                     /locus_tag="PKN52_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98509.1"
                     /translation="MAWDNGSITSDTPGAALSDKLKTLVGSTSNWSFVENVPAGTGAG
                     QSGSASYSVDVFKCAGTGAGANSAGIDFYVGVACYASGNSVWIATFERYSPIVSDTNK
                     GMCACPIGQYKNPA"
     gene            complement(350..1003)
                     /locus_tag="PKN52_00840"
     CDS             complement(350..1003)
                     /locus_tag="PKN52_00840"
                     /inference="COORDINATES: protein motif:HMM:NF013661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HNQ98510.1"
                     /translation="MALPTNQQVKRAMVDYARKQQPPIKVGPGINLANNAWGPAARTL
                     AWRITRRAKDVKTTTRKTPELCRLVGFKVVPIVIEHDWKWARPLGRRAGKPPFIIVHN
                     QAGTGTTAAVHAYHKSIGMVGIAYHFHITLEGEVHRGRPEWAMGGHTLYYNHCIGVCF
                     EGNYDIRKSMPRAQREAGLALIADLKRRYGVPVKGHREMPLNSTSCPGKHFPLKEMR"
     gene            complement(1005..1262)
                     /locus_tag="PKN52_00845"
     CDS             complement(1005..1262)
                     /locus_tag="PKN52_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98511.1"
                     /translation="MADSVISGLTLQAEQQLRMVVREEMASAMDSWSHDRPPCENVTE
                     LSETVSRLEERIRVLMWLAGTSFTISATAATTLLIKLLTGG"
     gene            complement(1273..2592)
                     /locus_tag="PKN52_00850"
     CDS             complement(1273..2592)
                     /locus_tag="PKN52_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98512.1"
                     /translation="MYDVGRDSRGLHIFAQWGTWYYCSRPNSYQAFPDIHGRPGINAR
                     LYWELYDGIPEEDPHCIRLVEMQLLLGGWQVCNYSKHQISVYGRHTLDGPNLICPWRH
                     LVRRYGSPAVDPQTWPMWHKTPMRSLYFPEALQIRCVVLRMETIETGYYEQQQLVVPL
                     EELHYRDQAGMEDPPPAGVIVKTARVFGQKPKRYRDGRAVILVSDAIKDVLAGRGFKT
                     TGLNTGRAKTLAADQLYWNELPKDRREALDEANAMLGFDYFAWSDDTVTFQAPGAGPL
                     KKIDITDPRVTLGLSDDIDQTYSGVRVQYTNRLGRVREVIVKRRSAALGKLLRATTIS
                     APDSIKTARSARQVGQRWLKDHSTTTHSDRVTLRGIIPGFGDCLLTRPGWRVTLVGAR
                     GAAKKLKVTGVRLRPLEWEAELSFDIEPWKFERWLSANAAKAYTVRR"
     gene            complement(2592..3233)
                     /locus_tag="PKN52_00855"
     CDS             complement(2592..3233)
                     /locus_tag="PKN52_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98513.1"
                     /translation="MALACTVTAPLSISAALYGGMAASRLACQVQAGFDVRAVLLGET
                     FYAGMSCRAVFHMAELRPRLYGEHVTLPEPYLGDEDWTPLEPDISDLRGLSIKLAGAE
                     LQRALIHSLEIDLTDRGGPETAIMTIHHDSRSLPIEMLRSLTCTYKGQRLFKGRLEGR
                     TRDLGGVMTTTLTFKGTLRKLQDHAAFRTCYVDSDLEHWRMTQPSSNANVNID"
     gene            complement(3243..3551)
                     /locus_tag="PKN52_00860"
     CDS             complement(3243..3551)
                     /locus_tag="PKN52_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98514.1"
                     /translation="MPDAGTPGTPVDYTGYLGPVEVAAADWEADDAGKLTNDAAIDFG
                     EPTDGADTAVAVEAYDDDQKTNRIWYEELDAPLDIDDGLDAVEFPIGGLSVTFGSSNM
                     "
     gene            complement(3666..4109)
                     /locus_tag="PKN52_00865"
     CDS             complement(3666..4109)
                     /locus_tag="PKN52_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98515.1"
                     /translation="MPEFTVVKWGGISFLGSHVNGLGIVGDIRITPLRRTIEVAANAL
                     SAVPVEVTDYYSDFGTIAFRARISGKASIGETAPECMRRLWKLLVIEARRPTNTLQVN
                     LQGVAEPLTYSLVKTTDEFERVIGKRTQSRSVIELDVSLRYVASS"
     gene            complement(4102..5697)
                     /locus_tag="PKN52_00870"
     CDS             complement(4102..5697)
                     /locus_tag="PKN52_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98516.1"
                     /translation="MGMTETLRLVIDGVPSGAKRALGEVRSEATKTEKTLSGINAGLN
                     KAANIAAVGVAAGVGVMAKSLKTYEDYSSQVRSIQRALGADAEGASRLAGQWKRYGVD
                     AGAATMATKTLAKAIDEARRGNAAYTETFNRLGLSIDQLKSMSDTEAIFAVRDALGEL
                     EAGAERTAIATKLLGRGAMSMSAWYSQSASDMARTNELLEKAGLVWTEEQVKKSGEAA
                     KKMAELQVAILGLQIQIAQSGVVDSMIHLVDGLTALLSRLGPLARALPYVTAGLAAFV
                     GVVKVISFVQFVRGLFSATSGVQALGAGTRGALPALTRLSTLLRGLPGLLSSTGGMYA
                     LVAAGIAADTYLIIKAIDAWRQMADAVRQARQAYADYLALTSQAEYQPGGKYYEGFKT
                     LPGAQTMEQINAAAKANQFGYSWWYKMASGAYSSLLGPYGALFSKLGSGILPGFASGG
                     TVKARRGGRLVLAGEGGEDEDFVPASQRVAYASRILGSGARTVQNFTINIASLQGTDE
                     RAARQLCEMVERRLSRRLRFAHA"
BASE COUNT          860 a         1918 c         1983 g         1022 t
ORIGIN      
        1 atggccgggt tcttgtactg cccgatggga caggcgcaca tgcccttgtt cgtatcggac
       61 acgatggggg agtaacgttc gaacgtcgct atccaaacac tgttaccgct cgcgtaacag
      121 gccaccccca cgtagaagtc gattcctgcg ctgttcgccc ccgccccggt cccggcgcat
      181 ttgaacacgt cgacggagta gctcgcgctg ccgctctgcc ccgccccggt cccggcgggc
      241 acgttctcga cgaacgacca gttgctggtc gagccgacca gcgtcttgag cttgtccgag
      301 agcgccgcgc cgggcgtgtc ggaggtgatg gaaccgttgt cccaagccat tacctcatct
      361 ccttcaaggg gaagtgcttg ccggggcacg aggtcgagtt gagcggcatc tcacggtgcc
      421 ccttgaccgg cacgccgtaa cgccgcttca ggtcagcgat gagcgccagg ccggcctcac
      481 gctgtgctct cggcatcgac ttgcggatgt cgtagttgcc ttcaaagcag acgccgatgc
      541 agtggttgta gtagagcgtg tgaccaccca tcgcccactc gggacggccc cggtggacct
      601 cgccctccag tgtgatgtgg aagtggtaag cgatgccgac catgccgatg ctcttgtggt
      661 aggcgtggac tgcagccgtc gtgccggtcc cggcctggtt gtggacgatg atgaacggcg
      721 gcttaccggc ccggcgtccg agcggccgtg cccacttcca gtcatgctcg atgacgatgg
      781 ggacgacctt gaacccgacc agacggcaga gctccggcgt cttgcgcgtg gtcgtcttga
      841 cgtccttggc gcgccgcgtg atgcgccagg ccagcgtgcg cgccgccgga ccccacgcgt
      901 tattggcgag attgatgccc ggaccgacct tgatcggcgg ctgctgtttg cgtgcgtagt
      961 cgaccatggc gcgcttgact tgctggttcg tcggcagagc catctcagcc tcccgtgagc
     1021 agcttgatga gcagcgtcgt cgccgccgtg gcggagatcg tgaacgaagt gccggccagc
     1081 cacatcagca cgcggatgcg ctcctcaagg cgactgaccg tctccgagag ctcggtcacg
     1141 ttctcacagg gcggccggtc gtgagaccag ctgtccatgg cgctcgccat ctcctcacgg
     1201 accaccatcc tgagctgctg ctcggcctgc agagtgagtc cgctgatcac actgtctgcc
     1261 acgatcaccc ccttatcgcc ttaccgtgta ggctttggcc gcgttggcgg acagccagcg
     1321 ctcgaacttc cagggttcga tgtcgaagga gagctccgcc tcccactcca gcggccgcag
     1381 ccgcacgccg gtcaccttga gcttctttgc ggcgccgcgc gcgccgacca gagtgacccg
     1441 ccaaccagga cgcgtgagca ggcagtcgcc gaatcccggg atgatgcccc ggagcgtcac
     1501 gcggtcggaa tgcgtcgtcg tcgagtggtc cttcaaccag cgctgcccga cctgccgcgc
     1561 gctgcgcgcc gtcttgatcg agtccggtgc cgagatggtg gtcgcccgca gcagcttgcc
     1621 gagcgcggcc gagcggcgct tgacgatcac ctctcgcacc ctgccgagac ggttggtgta
     1681 ctgcacccgc acgccggagt aggtctggtc gatgtcgtcc gagaggccca gtgtgacgcg
     1741 cgggtcggtg atgtcgatct tcttcagcgg tccggccccc ggcgcctgga aggtgaccgt
     1801 gtcgtccgac caggcgaagt agtcgaaccc gagcatcgcg ttggcctcgt cgagcgcttc
     1861 acggcgatcc ttgggcagct cgttccagta gagctggtcg gcggccagcg tcttggcgcg
     1921 gccggtgttg agaccggtcg tcttgaaacc gcgcccggcg aggacgtcct tgatcgcgtc
     1981 gctgaccagg atcacggcgc ggccgtcgcg gtagcgcttc ggcttctggc cgaagacgcg
     2041 cgccgtcttg acgatcacgc cggccggggg agggtcctcc atgccggcct ggtctcgata
     2101 gtgcagctct tcaagcggaa cgaccagttg ttgctgctcg taatagcccg tctcgatggt
     2161 ctccatgcgc agcaccacgc agcggatctg cagcgcctcg gggaagtaga gcgagcgcat
     2221 cggcgtcttg tgccacatcg gccaggtctg cgggtcgacc gccggagagc cgtagcggcg
     2281 cacgagatgc cgccagggac agatcaggtt gggaccgtcg agcgtgtgcc ggccgtagac
     2341 gctgatctga tgcttcgagt agttgcagac ctgccagccg cccagcagga gctgcatctc
     2401 gaccaggcgg atgcagtgcg gatcctcctc cgggatgccg tcgtagagct cccagtagag
     2461 ccgcgcgttg atgcccggtc gtccgtgtat gtcggggaag gcctggtagc tgttcggccg
     2521 gctgcagtag taccaggtgc cccactgcgc gaagatgtgc agcccgcggc tgtctctgcc
     2581 gacgtcgtac atcagtcgat gttcacgttg gcgttgctgg agggctgggt catgcgccag
     2641 tgctccagat cggagtcgac gtagcaggtg cggaaggcgg cgtggtcctg cagcttacgc
     2701 agcgtgccct tgaaggtcag cgtggtcgtc atcacgccac cgaggtcgcg cgtgcggccc
     2761 tcgaggcgtc ctttgaagag acgctggcct ttgtaggtgc aggtcagact ccggagcatc
     2821 tcgatcggca gcgagcgcga gtcgtggtgg atcgtcatga tggccgtctc cggcccgccg
     2881 cgatcggtca ggtcgatctc gagcgagtgg atcagggccc gctgcagctc ggcgccggcc
     2941 agcttgatcg agaggccgcg caggtcggag atgtccggct ccagcggcgt ccagtcctcg
     3001 tcgccgaggt agggctcagg cagggtcacg tgctcgccgt agagccgcgg ccgtaactcc
     3061 gccatgtgga agaccgcgcg gcagctcatg cccgcgtaga acgtctcgcc gagcagcacc
     3121 gcccgcacgt cgaagcctgc ctgcacctgg caggcgagcc gcgaggcggc catcccgccg
     3181 tagagcgcgg cggaaatcga gagcggagcg gtgactgtgc aggcgagcgc cacggagcta
     3241 tctcacatgt tcgatgatcc gaaggtgacc gacagaccgc cgatcgggaa ctcgaccgcg
     3301 tcgagcccgt cgtcgatgtc gagcggcgcg tcgagttcct cgtaccagat gcggttggtc
     3361 ttctggtcgt cgtcgtaagc ctcgaccgcg accgccgtat cggcgccgtc ggtcggctca
     3421 ccgaagtcga tcgcggcatc gttggtcagc ttgccggcgt cgtcggcctc ccagtcggcg
     3481 gcggcgacct ccaccgggcc caggtagccg gtatagtcaa ccggcgtgcc gggcgtaccg
     3541 gcgtcgggca cgtcggaggt cagctccagg taccatgagc tgggcccctg gtaggcgctg
     3601 ccgccgatcg cagcggcggc gatcttggcg gcctcggctg ggtcgagtgg catgtcagtc
     3661 tcctttcacg acgaggccac gtaacgcagg ctcacgtcca gctcgatcac ggagcggctc
     3721 tgcgtgcgct ttccgatcac gcgctcgaac tcatcggtcg tcttgaccag gctgtaggtc
     3781 agcggctcgg ccacgccctg caggttgacc tgcagcgtgt tggtcggccg ccgcgcctcg
     3841 atcacgagca gcttccagag gcggcgcatg cactccggcg cggtctcgcc gatcgaggcc
     3901 ttgccgctga tgcgtgcccg gaaggcgatc gtgccgaagt cggagtagta gtcggtcacc
     3961 tcgacgggga ccgccgagag tgcgttggcg gccacctcga tcgtccggcg cagcggcgtg
     4021 atgcggatgt cgccgacgat gccgagcccg ttcacatgcg agcccaggaa cgagatgccg
     4081 ccccatttga cgaccgtgaa ctcaggcatg ggcgaacctc agccgcctgg acaggcggcg
     4141 ctcgaccatc tcgcagagct ggcgcgcggc gcgctcgtcg gtgccctgca gggaggcgat
     4201 gttgatggtg aagttctgca ccgtgcgcgc gccggagccg aggatccggc tggcgtaggc
     4261 gacccgctgg ctggccggca cgaagtcctc gtcctcgccg ccctcgccgg ccagcacgag
     4321 ccgcccgccg cggcgcgcct tgacggtgcc gccactggcg aagccgggca ggatgccgga
     4381 tccgagcttg ctgaacagag ccccgtacgg accgagcagg ctcgagtaag ccccggaggc
     4441 catcttgtac caccaggaat agccgaactg gttagctttg gcggcggcgt tgatctgctc
     4501 catcgtctga gcgccgggca gggtcttgaa gccctcgtag tacttgccgc cgggctggta
     4561 ttcggcctgt gatgtgagcg ccaggtaatc ggcgtaggcc tggcgggcct gcctgacggc
     4621 gtcggccatc tgcctccagg catcgatcgc cttgatgatc aggtaggtgt cggctgcgat
     4681 cccggcggcc accagggcgt acatgccgcc ggtactggaa agcaatccgg gcagcccgcg
     4741 cagcagggtc gagagtcggg tcagggcggg cagcgcccct ctggtgccgg ctccgagcgc
     4801 ctgcacgccg ctggtggccg agaacagccc ccggacgaac tgcacgaagc tgatcacctt
     4861 gacgacgccg acgaaggcgg ccagtcctgc ggtcacgtag ggcaaggcgc gggccagcgg
     4921 cccgagccgg ctgagcagcg cggtgagccc gtcgacgagg tggatcatgc tgtcgaccac
     4981 gccgctctgc gcgatctgga tctggagccc gaggatggcg acctgcagct cagccatctt
     5041 cttggccgcc tcgcccgact ttttgacctg ttcctcagtc cagaccaggc ccgctttctc
     5101 gagcagctcg ttggtgcggg ccatgtcgct ggccgactgc gagtaccagg cgctcatcga
     5161 catggcgccg cggccgagca gcttggtggc gatggcggtg cgctcggcgc cggcctcgag
     5221 ctcgccgagc gcgtcgcgca cggcgaagat cgcctcggtg tcgctcatgc tcttgagctg
     5281 gtcgatgctc aggccgaggc ggttgaaggt ctcggtgtag gcggcgttgc cgcgccgcgc
     5341 ctcgtcgatc gccttggcca gcgtcttggt cgccatggtg gcagcgccgg cgtcgacgcc
     5401 gtaacgcttc cactgaccgg ccagccgtga ggcgccctcg gcgtcggcgc cgagcgcgcg
     5461 ctggatgctg cgcacctgcg agctgtagtc ctcgtaggtc ttcaggctct tagccatgac
     5521 cccgacgccg gcggccactc cgacggcggc gatgttggcc gccttgttga ggccggcgtt
     5581 gatgccgctc agggtcttct ctgtcttggt cgcctcggag cggacctcac cgagcgcgcg
     5641 cttcgccccc gacgggactc cgtcgatgac caggcgcaac gtctcggtca tgcccatggc
     5701 gcctccctta gaaaaccgtc agccccagca gcatgacgac gaactcacgg tcgcggtcgc
     5761 gcaggtcgcc cacggtgagg tgg
//
LOCUS       DAOQYE010000021         5463 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_15223_length_5463_cov_2.810466, whole genome shotgun sequence.
ACCESSION   DAOQYE010000021 DAOQYE010000000
VERSION     DAOQYE010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 5463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5463
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15223_length_5463_cov_2.810466"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            118..1101
                     /locus_tag="PKN52_00875"
     CDS             118..1101
                     /locus_tag="PKN52_00875"
                     /inference="COORDINATES: protein motif:HMM:TIGR02136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HNQ98517.1"
                     /translation="MQMPLKPLRPFLTLVAITFGLSAAAQGTIVVDGSSTVYPISLAM
                     AEEFTIDNPSIPVSVGFSGTGGGLSKICAGEIDIADASRVVKPSELEACAANGIELIE
                     LPVAADALTVVVNKQNDFASCLTVAELHAIWAPDSQVTSWSQVRADFPDAPITLFGPG
                     TDSGTFEYFTEAVNGKAGAIRTDFFPSEDDNVLVRGVESDTYAMGYFGFAYYAADAER
                     LNAVSVDGGNGCVAPSAATIEDGTYSPLSRPLFIYVSLKSLETKPGLAEFVEYYLSQD
                     ARELVEHTGYATYSDEIYDAVAARFSARTTGTAFAGFEPGDSVLDAVKGIE"
     gene            1118..2059
                     /gene="pstC"
                     /locus_tag="PKN52_00880"
     CDS             1118..2059
                     /gene="pstC"
                     /locus_tag="PKN52_00880"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HNQ98518.1"
                     /translation="MSTAGGGGNGMGRALRLSRSETRRERLVAILLGLAALLTVLTTV
                     AVVIVLARETFEFFRDPLVSVWEFLTGDRWTPLFADKHFGILPLVTGTLLVTVLAVLV
                     GVPMGLASAIYLSEYAPPARRRTLKGILELLAGIPTVVLGFFALSYVTPVLQGLIPGV
                     KFFNALSAGLVMGVMILPTVSSLASDALQAVPLSLREAGYGLGGSKLDVILKVLLPAA
                     FSGLAAAVILAFSRAVGETMIVSLAAGQRPILTFDPRETIATMTSFIVQAATGDQPVG
                     SLAARSLYAVGAVLFGLTLLMNLASQALVRRFRERYE"
     gene            2107..3249
                     /gene="pstA"
                     /locus_tag="PKN52_00885"
     CDS             2107..3249
                     /gene="pstA"
                     /locus_tag="PKN52_00885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HNQ98519.1"
                     /translation="MAVPTVLALALLAALMLELLSDTVSWQVVEPRGSGETFSFLDGF
                     SITSSWEKVVTLELAARGSDQAEIDALIRDPEERRKFAARNRVELMLWADGKPLRWVV
                     TGGRDVLVQDRGLLEGWRQRDELSAGLKPGQHLYLNPWLDISFFTKSASRTPLMAGLA
                     PALLGSLWIIGLVMLIAVPLGVGAAIYLEEYAGDNAFTRLLEVNLRNLAGVPSIVYGI
                     LGLTVFVRLLGLGPVILAAALTLSLLVVPVVVIASREGIRGVPNSLRQAAYGLGATRS
                     QVVFRVVLPAALSSVVTGVILAVARALGETAPLLVIGAAVFVPGLPNGPLAQYTALPI
                     QIYTWVGENEREFAHVASAGIVVLLAVLSLLYGAAFWLRRKYEKRW"
     gene            3349..4080
                     /gene="pstB"
                     /locus_tag="PKN52_00890"
     CDS             3349..4080
                     /gene="pstB"
                     /locus_tag="PKN52_00890"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HNQ98520.1"
                     /translation="MDSFSLWYGGFKALKSVSLTVPRGQVTALIGASGCGKSTLLRSI
                     NRMNDLVDGVRTEGRVLYEGHELYGVGVDPVEVRRRVGMVFQKPNPFPKSIFDNVAFG
                     PRLIGRTSDLGAVVERALKDAALWDEVHGNLKGSAFGLSGGQQQRLCIARALATEPAV
                     LLMDEPTSALDPIATDRIERLIADLKGKYTVIIVTHNMQQAGRVSDRTAFMHLGTLVE
                     EGPTDQIFTAPKHELTERYVSGRFG"
     gene            complement(4056..4691)
                     /gene="pth"
                     /locus_tag="PKN52_00895"
     CDS             complement(4056..4691)
                     /gene="pth"
                     /locus_tag="PKN52_00895"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HNQ98521.1"
                     /translation="MTQPSGIAPRVRLVVGLGNPGPRYSGTRHNAGFFVVDELARRHG
                     GSFRQEREAASAKLAPGTLGSGASQLLKPLTFMNLSGRAVQAAMTRGGARPEELLVVH
                     DDIDLPLGRLRIRFGGSGGGQRGVADITRAIGPGFWRLKVGVGRPPEGWATESWVLSR
                     FAEVEKALLERVVASAADAVELVIRGGAERAMNEVNGLDLAEPPQPNRPET"
     gene            complement(4694..5302)
                     /locus_tag="PKN52_00900"
     CDS             complement(4694..5302)
                     /locus_tag="PKN52_00900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HNQ98522.1"
                     /translation="MRIEAEKRAPGTAAQLRRDGKLPAVVYNKDLNESITLDMRAFDK
                     AFRAQGTSSLIDLVVDGTEHSVLVKQVQMDKRRREPMHVDFFAVTANEALEVHVPIEV
                     VGTAAGVREGGLLDVQRREVKISVLPRFIPNHVTLDVSAVKVGESLHVRDLVAALPKE
                     ATVLDDLDLSVVAVVPPRVSDDAGSSTATEPEVIRSAAEEND"
BASE COUNT          849 a         1855 c         1781 g          978 t
ORIGIN      
        1 cccatcggca tgtgtctagc ctccagagtc cttcaagtca ggtcattatc gcccgaccga
       61 ccggcccatg acggagccat gacctggcgt acgtaccctc cgaggtggag gttggccatg
      121 cagatgccac tgaagcccct tagacccttc ctgaccctag tagcgattac gttcggcctg
      181 agcgccgccg cgcaaggaac gatcgtcgtg gacggctcct cgaccgtcta tccgatctcc
      241 ctcgccatgg cagaagagtt caccatcgac aacccctcca tccccgtctc ggtcggcttc
      301 tccggtaccg gaggcggact ctccaagatc tgcgcgggcg agatcgacat cgcggacgcc
      361 agccgcgtgg tgaagcccag cgagctggag gcgtgcgcgg ccaacggtat cgagctgatc
      421 gagctgccgg tcgcggcaga cgcgctcacg gtcgtggtca acaagcagaa cgacttcgcg
      481 agctgcctca cggtcgccga actgcatgcg atctgggccc ccgactcgca ggtcacgagc
      541 tggagccagg tgcgcgccga cttccccgac gcccccatca cgctcttcgg cccaggcacc
      601 gacagcggca cgttcgagta cttcacggaa gcggtcaacg gcaaggctgg cgccatccgc
      661 accgacttct tcccgagcga ggacgacaac gtgctcgtcc gcggtgtcga gagcgatact
      721 tacgccatgg gctacttcgg gttcgcctac tacgcggccg atgccgagag gctcaacgcc
      781 gtgtctgtcg acggcggcaa cggctgcgtc gcgcccagcg ctgccacgat cgaggacggc
      841 acctatagcc cgctctcgcg cccgctgttc atctacgtca gcctgaagtc cctcgagacc
      901 aagccgggtc tcgccgagtt cgtcgagtac tacctctcgc aagacgcgcg tgaactggtc
      961 gagcacacgg gttacgccac ctactccgac gagatctacg atgcggtcgc cgcgcgcttc
     1021 tccgcgcgca ccaccggcac cgcgttcgct ggcttcgagc ctggcgacag cgtgctcgat
     1081 gccgtcaagg gcatcgagta gtcgaggcga aacccacgtg agtacggccg gcggtggtgg
     1141 gaacggtatg gggcgcgcct tacggttgag ccgctccgag acgcggcgtg agcgcctggt
     1201 ggcgatactc ctcgggctag ccgcgttgtt gaccgtcctt accaccgtgg ccgtcgtgat
     1261 cgttcttgcg cgggagacct tcgagttctt ccgcgatccg ctcgtcagcg tctgggagtt
     1321 cctcacgggc gaccgctgga cgcccctgtt cgccgacaag catttcggca tcctgccact
     1381 ggtcaccggt acgctgctcg ttaccgtcct cgcggtgttg gtaggggtgc ctatgggact
     1441 ggccagcgcc atctacctgt cggagtacgc cccgcctgcc agacggagga cgctcaaagg
     1501 gatactcgag ctcctggcgg gtatccccac ggtggtgctc ggtttctttg ccctctctta
     1561 cgtgacgccc gtgttgcaag gcctcatacc cggcgtcaag ttcttcaacg ctctttccgc
     1621 tggcctcgtc atgggtgtca tgatcctccc caccgtctcg agcctggcga gcgatgcctt
     1681 acaggccgtt ccactgtcgt tgcgcgaggc ggggtacggg ctcggaggca gcaagttgga
     1741 cgtgatcctg aaggtcctgc ttcccgccgc gttctccggg cttgcggccg cggtcatcct
     1801 ggcgttctcg cgcgccgtgg gggagaccat gatcgtcagc ctggccgccg gccagcgtcc
     1861 catcctcacg ttcgacccac gcgagaccat cgccaccatg acctccttca tcgtgcaggc
     1921 cgcgaccggc gaccaaccgg tcgggtcgct agcggcccgc tccctctacg cggtcggcgc
     1981 ggtgctcttc ggcttgacgc tgctgatgaa cctcgcctcg caggccttgg tcaggcgctt
     2041 cagagagcgc tacgaatgag ggcggcgcgc ggtagacgac tgcgcggaag agccttccat
     2101 gccgcggtgg ccgttccgac cgtcctggcg ctggccctgc tggccgcgct catgctcgaa
     2161 ctcctctccg acacggtctc gtggcaggtc gtggagcccc gtggcagcgg cgagactttc
     2221 tccttccttg acggcttctc gatcacgagc tcctgggaga aggtcgtgac ccttgaactc
     2281 gccgcgaggg ggagcgacca ggcggagatc gatgccctca tacgcgatcc tgaggagcgc
     2341 cgtaagttcg cggcgcgtaa ccgtgtcgaa ctcatgctgt gggcggacgg caagccgctg
     2401 cgttgggtcg tgaccggcgg ccgcgacgtc ctcgtgcaag accgcgggct gctcgagggc
     2461 tggcggcaac gcgatgagct atcggctggt ttgaagcctg gtcagcacct ctatctgaac
     2521 ccatggctgg atatctcctt cttcaccaag tcggcctcca ggacgcccct catggccggc
     2581 ttggcccccg cgctgctcgg cagcctctgg atcatcgggc tggtcatgct gatagccgta
     2641 ccgctcgggg tcggggccgc catctacctc gaggagtacg ccggcgataa cgccttcacg
     2701 cgccttctcg aggtcaacct ccgcaacctc gcaggggtgc cgagcatcgt ctacgggatc
     2761 ctgggcctta ccgtgttcgt acgtctgctc ggtctcggtc ccgtgatcct ggccgcggcc
     2821 ctcaccctgt ccttgctcgt cgtccccgtg gtcgtcatag cctcgcgcga gggtatcagg
     2881 ggcgtaccga actccttgcg gcaggccgcg tacggtctcg gcgccacgcg ctcgcaggtc
     2941 gtgtttcgag tggtgctgcc cgccgcactt tcgagcgtcg tgaccggtgt catcctggcc
     3001 gtcgccaggg ccctcggcga gacggcgccg ctgctcgtca tcggggccgc ggtcttcgtc
     3061 cctgggctgc cgaacggccc gctggcgcaa tatactgcgc tccccataca gatctacacg
     3121 tgggtgggag agaacgaacg cgagttcgcg cacgtcgctt ccgccggcat agtcgtcctc
     3181 ctggccgtgc tgagccttct atacggggcg gcgttctggc tcaggcgcaa gtacgagaag
     3241 cgttggtgaa gctagcgtga tgggaaggat agcgtgagca aggcattacc ggaagaccac
     3301 tcgaccgtcc gcaccgaccc ggtgtcggtc gacgaagcgg tcgtcgccat cgactcgttc
     3361 agcctctggt acggcgggtt caaggccctg aagagcgtgt cgctgacggt gccgcgcggc
     3421 caggtcacag cgctcatcgg cgcgtcgggt tgcggtaaga gcacgctgct gcgcagcatc
     3481 aaccgcatga acgacctggt ggacggcgtc cgcacggaag gacgggtgct ctacgaagga
     3541 catgagctgt acggcgttgg cgtcgacccg gtcgaagtgc ggcgccgggt cggcatggtg
     3601 ttccagaagc ccaacccttt ccccaagtca atcttcgaca acgtggcgtt cggaccgcgc
     3661 ctcatcggcc gcacgagcga cctgggcgcg gtggtcgagc gcgccttgaa ggacgcggcg
     3721 ctgtgggatg aagtacatgg caacctgaag ggctcggcct tcgggctctc gggcggtcag
     3781 caacagcgcc tctgcatagc gcgcgcgttg gcgaccgagc cggccgtgct gctgatggac
     3841 gagccgacca gcgcgctcga cccgatcgcg accgatagga tcgagcgcct gatagccgac
     3901 ctcaagggga agtacaccgt gatcatcgtc acccacaaca tgcagcaagc cggccgggtg
     3961 agcgaccgca cagccttcat gcacctcggg actctcgtgg aggagggccc gacggatcag
     4021 atcttcaccg cgcccaagca cgagctgaca gaacgctacg tctccggacg gttcggctga
     4081 ggaggctcgg cgagatccag gccgttgacc tcgttcatcg cccgttccgc gccgcccctg
     4141 atgacgagct cgacggcgtc ggccgcgctc gccacgacgc gctccagtaa ggccttctcg
     4201 acctcggcga agcggctcag cacccagctc tccgtcgccc agccttccgg agggcgtccg
     4261 accccgacct tcagccgcca gaagccaggg ccgatggcgc gggtgatgtc cgccacgcca
     4321 cgttgcccgc ccccgctgcc gccgaacctg atgcggagtc ggccaagcgg taggtcgatg
     4381 tcgtcgtgca caacgaggag ttcctcgggc cgcgcgccac ccctcgtcat ggcggcctgc
     4441 acggccctac cggagaggtt catgaacgtc agaggtttca acagttgaga ggcgcccgat
     4501 ccgagggtgc ccggcgccag cttggcgctt gccgcctcgc gctcttgcct gaacgagccg
     4561 ccgtgtctgc gagccagctc gtcaacgacg aagaacccgg cgttatgccg ggttcctgag
     4621 taacgcgggc ctgggttgcc caggcctact acgagcctta cccgaggcgc tatgcccgag
     4681 ggttgggtca accctagtcg ttctcctcgg cggccgagcg gatgacctcg ggctcggtgg
     4741 ccgtggagga gccggcgtca tcggagacac ggggcggcac gacggccacg acggagaggt
     4801 cgaggtcgtc gagcacggtg gcttccttgg ggagcgcggc gacgaggtcc cttacgtgca
     4861 ggctctcgcc gaccttgacg gccgacacgt cgagagtgac gtggttcggg atgaagcggg
     4921 ggaggaccga gatcttgacc tcacgccgtt gaacgtccag gaggccgccc tcccgcacgc
     4981 cggcggcggt gccgacgacc tcgatgggca cgtggacttc cagggcctcg ttggcggtca
     5041 cggcgaagaa gtcgacgtgc atcggctcgc gacgccgctt gtccatctgg acctgcttca
     5101 cgaggacgga gtgctccgtg ccgtcgacca cgaggtcgat gagggaagag gtcccctgcg
     5161 cgcggaacgc cttgtcgaac gcccgcatgt cgagggtgat ggactcgttg aggtccttgt
     5221 tgtagaccac cgcgggaagc ttcccgtcgc ggcgcaactg cgcggccgtg cccggcgcgc
     5281 gcttctcggc ttcgattctc atggttcctc cggcgcgtct acccttgagc ggcgacgcga
     5341 tcgcaactag tgacgatact cgttatcacc cgtgtctgta aagacactcg cgccctgcgg
     5401 atggcggtgg gaacgactcg cccctccctg gcaccagccg gcgctgcgcg cgcgtctcga
     5461 acg
//
LOCUS       DAOQYE010000022         5450 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_15272_length_5450_cov_3.388879, whole genome shotgun sequence.
ACCESSION   DAOQYE010000022 DAOQYE010000000
VERSION     DAOQYE010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 5450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5450
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15272_length_5450_cov_3.388879"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="PKN52_00905"
     CDS             <1..276
                     /locus_tag="PKN52_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98523.1"
                     /translation="RACSASSTTASEGGASGGTGLVAMRERVRALGGTVTVNPQTSGA
                     KQGTRLEVSLPRRAAIAAPGVAARDAGAAANATADAAAAAPARPAKA"
     gene            273..887
                     /locus_tag="PKN52_00910"
     CDS             273..887
                     /locus_tag="PKN52_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNQ98524.1"
                     /translation="MIRVVIAEDQAMVLGALAALLGLEDDIEIVAEARDGAEALARVR
                     ESRPDVLLTDIEMPRLTGIELASAVRDERDRAGLTTRVVILTTFARAGYLRRALEAGA
                     VGYLLKDSPAEKLADAIRRVHAGGRAVDPELAVEALGEVDPLTDRERQVLRLAGEGVS
                     TAAIASTLGLSEGTVRNYLSEAISKLGVGNRVEAARLARSRGWL"
     gene            complement(972..1655)
                     /locus_tag="PKN52_00915"
     CDS             complement(972..1655)
                     /locus_tag="PKN52_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98525.1"
                     /translation="MAKPRWAEQLTSLDRESWPAFLDRHSGLPGPRANLELATVFATL
                     ADEALVEELANDTDEYRAMCGAAALGRRAADPAAEARVRELAGDERWRVRMGVELGLQ
                     LLGAEAPNALRSVVVDWVEDADPLVKRAAVAAICEPRLLRDPECAAVAIAVCRRATDA
                     IAAMPPELRREPDTRVLRQALGYCWSVAVAADPAPGLAAFARLDTSDPDVAWIAKENR
                     GKRRLATLL"
     gene            1763..2317
                     /locus_tag="PKN52_00920"
     CDS             1763..2317
                     /locus_tag="PKN52_00920"
                     /inference="COORDINATES: protein motif:HMM:NF015674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HNQ98526.1"
                     /translation="MLARTWWTTLLRGIALVIFGAIALLWPGLTLVALVYTYAVFALV
                     DGAFSLASLVARTGVGPWWAQLFGGLLSIAAGIVALVMPGLTVITFIYLIAAWAVLRG
                     FMDIVVAVALRKEITNEWSLILAGLVSIVFGVVLAVWPGAGLVTFVALLAAFALVAGV
                     SLIVLAFRLKGANDRRESARQARA"
     gene            complement(2563..4242)
                     /locus_tag="PKN52_00925"
     CDS             complement(2563..4242)
                     /locus_tag="PKN52_00925"
                     /inference="COORDINATES: protein motif:HMM:NF012343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HNQ98527.1"
                     /translation="MTSYRTQRLSLRFFLLMLVLLLVQVALGVLLSLQHADPTLLSGT
                     MNFNITRSQHVNLGVLWILSGFIGTILFVGPLLSKRELAAPWLIKFLFVALVAVTVWN
                     AVAIFFAQKGIAGWWLGQAWIQEGLEYLEAGRIADIIILIGFAILAYVVLRTFPKRSE
                     WNEIHWGLAIGVTALTVMWVFGMFFIGRIDLQEYFRWFVVHYWVEGVWEVIHISLIGI
                     LLVLMFKADIKAVGYAVFWGVTLVWLSGLLGNAHHYFWVGTPEFWQFWGSLFSALEPL
                     PLILCFWHMYLDAHHDRKPIENKPAFYFLLGSAVLEMVGAGILGFTMTFALTNVWSHG
                     TWVTPSHAHLSMFGTFGMLGLGAAYFIVPKVRKVVDFDQRFGQLAFWLVFLGMLGIGF
                     AFAIGGTIQVFTYRTLGLDWFGGDIYPAMGTYKILLVLFGIVFFAGVLLLVFDLITLG
                     HRVQAPAPQATDYAPPTTGWARPLRGFEAGIWLLGMWVFGLIITFGLLSYNLPRVQGG
                     DPTIPYTAAAVGYPGLLLTTLLFVWRFLASLEARERASSVAMQRRTALAGD"
     gene            complement(4239..5306)
                     /locus_tag="PKN52_00930"
     CDS             complement(4239..5306)
                     /locus_tag="PKN52_00930"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNQ98528.1"
                     /translation="MAGNAVRVETSRRRAALLILGLMVIVFIILSAFSFVGSRDQITP
                     DTVTYADTFGAVDGKRVFQAYNCMGCHTIVGNGAYLGPDLTNTYESAGPAWIAAFLPS
                     AGTWPTEAALRVQLQNSNQVAETGITDLAAYYEAYPGAKERIERRGGLPTFMPNLGFR
                     AGEVSEVLAFLKYTSLMDTEGWPPTPKVDGLEFPQAAKVINVASTRPTATDTRNAPAT
                     SGSSPSATTAGGSESGTMAAQTPVGDAPAEAGSATTSEADPAAGLALADQYGCFACHA
                     KTEARLVGPGWGGLYGSTQSLEGGGGATADEAFLAESITHPDAAIAAGYPAGVMPAYA
                     GILTDDEVAAIVAYIRSLETP"
BASE COUNT          825 a         1772 c         2043 g          810 t
ORIGIN      
        1 agggcctgca gcgcgtcgag cacaactgcg agtgagggcg gcgcttcggg cggtacgggg
       61 ctcgtcgcca tgcgcgagcg ggtgcgcgcg ttgggcggca cggtgacggt gaacccgcaa
      121 acgtccggcg cgaagcaggg cacgcgtctg gaggtcagtc tcccgcgccg ggccgccatc
      181 gccgcccctg gcgtcgccgc ccgtgacgcc ggcgccgcgg ccaacgctac ggccgacgcc
      241 gcggctgccg cgccggcgag accggccaag gcatgatccg cgtcgtcatc gccgaggacc
      301 aggccatggt gctgggcgcc ctggcggccc tcctcgggct ggaggacgac atcgagatcg
      361 tcgccgaggc ccgcgacggc gccgaggctc tcgccagggt cagggagtcg cggccagacg
      421 tgctcctgac ggacatcgag atgccgcgcc tcaccggcat cgaactcgcg agcgccgtga
      481 gggacgagcg cgaccgcgcc ggcctgacga cgcgagtcgt catcctcacc accttcgcgc
      541 gcgcaggcta cctccgcagg gcgctggagg ccggcgcggt cgggtacctg ctgaaggact
      601 cgccggcgga gaagttggcc gacgccatcc ggcgcgtgca cgcgggcggc cgggcggtgg
      661 atccggagct ggcggtggag gcgctcggcg aggtcgaccc gctgacggac cgcgagcgtc
      721 aggtgctcag gttggctggc gagggcgtct cgacggcggc catcgcgtcc acgctggggc
      781 tctcggaggg cacggtgcgc aactacctgt cggaggcgat cagcaagctg ggggtcggca
      841 accgcgtcga ggccgcgcgc ttggcaagga gcagaggctg gctgtgaggc gcatgggtcg
      901 ccgccgctcg cgcccgtgca ggctcggcga tcacggggcg tagctaggcc gcgcacctcg
      961 tcaccgccac gtcacagtag ggtcgccagc cgcctcttgc cccggttctc cttagcgatc
     1021 caggcgacgt ccggatcgga ggtgtcgagc ctggcgaacg cggccaggcc gggggccggg
     1081 tcggccgcga ccgcgacgct ccagcagtag ccgagcgcct ggcgcagcac gcgcgtgtcc
     1141 ggctcccgcc gcagttcggg tggcatggcg gcgatggcgt ccgtcgcgcg gcggcagacg
     1201 gcgatcgcga ccgccgcgca ctcggggtcg cgcaggagcc gcggctcgca gatcgcggcg
     1261 acggcggcgc gcttcaccaa tgggtcggcg tcctccaccc agtcgaccac gaccgaccgt
     1321 agcgcgttcg gcgcctcggc gccgaggagc tgcaggccca gctcgacgcc catgcggacc
     1381 cgccagcgct catcgcccgc cagctcccgc acccgcgcct cggcggcggg gtcggccgcc
     1441 cggcggccga gcgccgccgc gccgcacatg gcgcggtact cgtcggtgtc gttcgcgagc
     1501 tcttcgacga gcgcctcgtc cgccagggtc gcgaagacgg tcgccaactc gaggttcgcg
     1561 cgcggaccgg gcagaccgga gtgccggtcc aggaaggcgg gccatgactc cctgtcaagg
     1621 ctcgtcaact gctccgccca gcgcggcttg gccatggcgc atgctaccgg gtgcgcggga
     1681 acgtacatat aatcggggcc taggccggac cggacccggg tccgcggccg agttcgctca
     1741 gggaggctgc cggatgctcg acatgctcgc taggacatgg tggaccacgt tgctgagggg
     1801 gatcgcgctg gtgatcttcg gcgccatcgc gctcctgtgg ccggggctga cgttggtggc
     1861 cctcgtctac acgtacgcgg tgttcgcttt ggtggatggc gccttctcgc tcgcctcgct
     1921 cgtcgcccgc accggcgtcg gcccctggtg ggcccagctc tttggcggcc tgctcagcat
     1981 cgcggcaggc atcgtcgcgc tggtcatgcc ggggctgacg gtcatcacct tcatctacct
     2041 gatcgcagct tgggccgtgc tgcgcggttt catggacatc gtggtcgcgg tggccctgcg
     2101 caaggagatc acgaacgagt ggtcgttgat cctcgccggg ttggtgtcga tcgtcttcgg
     2161 cgtcgtgctg gccgtctggc cgggcgccgg cctggtcacg ttcgtcgcct tgctagccgc
     2221 gttcgcgctc gtggcgggcg tgtcgctgat cgtgctggcg ttccgcctca agggcgcgaa
     2281 cgaccgcagg gagtcggcgc gtcaggcacg cgcctgacac ggtgcgcgcc tgggttcggg
     2341 ccgccatggc cttggggtga ggtagggggc ggttgacgcc gctgccccgt gccgctagtg
     2401 aacgcatgac aggccccgcg accgaacggt cgcggggccg ttccgaccag gcgcaggccc
     2461 cacggcgccg gtcgaacgag gggagggtcg tcggcgccgg ccaggagaga cggcgccgac
     2521 ggcggtgggt ggcgcctggc gaaggcgccg cgcggctcgg cgctagtcgc ccgccaaggc
     2581 ggtccgcctt tgcatggcga cgctggaagc gcgttccctg gcctccaggg acgcgaggaa
     2641 gcgccagacg aagagcaagg tcgtgagcag caggcctggg taccctacgg ccgccgccgt
     2701 gtaggggatg gtcggatcgc cgccctgcac gcgtgggagg ttgtagctca acagcccgaa
     2761 cgtgatgatg aggccgaaca cccacatgcc gagcagccag atccccgcct cgaagccgcg
     2821 cagtgggcgg gcccagcccg tggtcggggg cgcgtagtcg gtcgcctgcg gtgccggtgc
     2881 ctggacccgg tggccgaggg tgatgaggtc gaagacgagc agcaggacgc ccgcgaagaa
     2941 cacgatgccg aagaggacga gcaggatctt gtaggtgccc atggcggggt agatgtcgcc
     3001 accgaaccag tcgagcccca acgtgcggta ggtgaagacc tggatcgtcc cgccgatggc
     3061 gaaggcgaac ccgatcccga gcatgccgag gaaaacgagc cagaaggcga gctggccgaa
     3121 gcgctggtcg aagtcgacga ccttgcgcac cttgggcacg atgaagtacg cggcgcccaa
     3181 gccgagcatg ccgaaggtgc cgaacatgga gaggtgcgcg tggctgggcg tcacccacgt
     3241 gccgtgcgac cagacgttgg tgagggcgaa cgtcatcgtg aagccgagga tgccggcgcc
     3301 gaccatctcg agcaccgccg agccgagtag gaagtagaag gcgggcttgt tctcgatggg
     3361 tttcctgtcg tggtgggcgt cgaggtacat gtgccagaag cacaggatga ggggcaacgg
     3421 ctccagcgcg ctgaagagcg agccccagaa ctgccagaac tccggcgttc cgacccagaa
     3481 gtagtggtgg gcgttgccga gaagaccgga gagccacacc agggtgacgc cccagaagac
     3541 cgcgtagccg acggccttga tgtcggcctt gaacatgagc acgagcagta tgccgatgag
     3601 gctgatgtgg atgacttccc agacgccctc aacccagtag tggacgacga accagcggaa
     3661 gtactcctgc aggtcgatgc ggccgatgaa gaacatgccg aagacccaca tgacggtgag
     3721 ggccgtgacc ccgatggcga gcccccaatg gatctcgttc cactcgctgc gcttgggaaa
     3781 ggtgcgcagc acgacgtagg ccaagatggc gaagccgatc aggatgatga tgtcggcgat
     3841 gcgcccggct tctaggtact ccaggccttc ctgtatccac gcctgtccca gccaccagcc
     3901 cgctatgccc ttctgggcga agaagatggc gacggcgttc cagacggtca ccgccaccaa
     3961 cgccacgaac aggaacttga tgagccaggg cgcagcgagc tcgcgcttag agagcagcgg
     4021 ccctacgaag aggatcgtgc cgatgaaacc ggagaggatc cagagcacgc ccaggttcac
     4081 gtgctgggaa cgggtgatgt tgaagttcat cgtgccgcta aggagggtcg ggtcggcatg
     4141 ctgcaaggac aggagcacgc ccagcgccac ctgtacgagc aacagcacga gcatcaggag
     4201 gaagaagcgc aggctcaaac gttgggtgcg gtaactcgtc atggcgtctc cagcgagcgg
     4261 atgtaggcga cgatcgcggc cacctcgtca tcggtgagga tgcccgcgta ggcgggcatg
     4321 accccggccg ggtagccggc ggctatggcc gcgtccgggt gggtgatcga ctcggcgagg
     4381 aacgcctcgt ccgccgtggc gccaccgccg ccctcgaggc tctgggtgct cccgtatagg
     4441 ccgccccagc cgggtccgac caggcgcgcc tccgtcttgg cgtggcaggc gaagcagccg
     4501 tattggtcgg cgagcgcgag gccggctgcc gggtcggctt cgctggtggt ggcggagccc
     4561 gcctccgcgg gagcatcgcc gaccggcgtc tgtgctgcca tggtgcccga ctcggatccg
     4621 ccggcggtcg tggcgctagg tgaggagccg ctggtcgccg gtgcgttccg ggtgtccgtg
     4681 gcggttgggc gggtcgaagc gacgttgatg accttggcgg cctgcgggaa ctcgaggccg
     4741 tcgaccttgg gcgtgggcgg ccagccctcc gtgtccatga gggacgtgta cttgaggaac
     4801 gcgagaacct cgctcacctc gcccgccctg aagccgaggt tgggcatgaa cgtgggcagc
     4861 ccgccgcggc gctcgatgcg ctccttggcg cccgggtagg cctcgtagta ggcggcgagg
     4921 tcggtgatgc cggtctcggc cacctggtta gagttctgga gttggacgcg gagcgcggcc
     4981 tcggtgggcc acgtgccggc cgagggcagg aacgcggcga tccaggccgg cccggccgat
     5041 tcgtacgtgt tcgtgaggtc gggccccagg taggcgccgt tgccgacgat cgtgtggcac
     5101 cccatgcagt tgtaggcctg gaagacgcgc ttgccgtcca cggcgccgaa cgtgtcggcg
     5161 tacgtcaccg tatcgggtgt gatctggtcg cgtgagccta cgaacgagaa cgccgacagg
     5221 atgatgaaga cgatcaccat gagcccgagg atcagcagcg cggcgcgccg gcggctggtc
     5281 tcgacgcgca cggcgttccc ggccatcagt ggcctcccgt tgtcatggct ccgtggccgc
     5341 cgtggtcgtg ctcgtcatgg tggtgctcgg ggtgctcgtg cccgtggtgc tcgtgcccgt
     5401 ggtggtcgtg cccatggtgg tcgtgcccgt cgtggctgaa gagggtacgc
//
LOCUS       DAOQYE010000023         5449 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_15276_length_5449_cov_4.028365, whole genome shotgun sequence.
ACCESSION   DAOQYE010000023 DAOQYE010000000
VERSION     DAOQYE010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 5449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5449
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15276_length_5449_cov_4.028365"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="PKN52_00935"
     CDS             <1..448
                     /locus_tag="PKN52_00935"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HNQ98529.1"
                     /translation="GRIPPFELDESITYHDPCYLGRHNGIYDAPRQVLESGGATLLEM
                     ERNRNDSFCCGAGGAQFWKEEEEGDGRVSNERFEEAVATGAQVIATGCPFCKVMLSSS
                     DGAEGETAPQVMDVAQLVAMRLDNIQALFDHHAGAGGAVGGSVSGD"
     gene            577..2904
                     /locus_tag="PKN52_00940"
     CDS             577..2904
                     /locus_tag="PKN52_00940"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNQ98530.1"
                     /translation="MDRLGYTVKASLGADGSESDGGAANQQRAGLGTMLLAEVFARVK
                     DALIVLDQADVVVAANPAAEALMQVRQGGLVGMPLVKLLPVSAPLLALVVAPDRRGSL
                     ELEVDLVRPQGGLVPASVSAFRLDDGRTVLVCHSPSIGVLDSRSPRAERRFRRMAESS
                     PDVRFVIDAHERIEYASPSAAELGLVAGEHTEADVWRLVHPRDRAAAQRALAARYDRS
                     AEPLEDVVVTVRARVGDGWRWFEARVTDLTDDPDIRGLLVQARDVTDRVEELKRLRAS
                     EAYLRAIFERNHDGLVLLDKRKHIVRLVGRSQEDLGYSASDLLGSRNVHLVHPDDREA
                     LSDAFDMVEARLGATEQVRLRVRDRAGAYHWIEGSLSNMLHDPDVRAFVLSYRSVGDH
                     GPTVDRVRSLNDQLTRRVQHLQSLRRIDMAINNSADLRLVFEIFMNQLVQDLGVDAVA
                     VLLYEPQLQQLRPFMGLGFLSELRVRTRVPLGEGPAGRAAADQHNVYVPDLRLGSVWG
                     AAIEPEHQTGFSSYMAVPMTAKGQLHGVLELYARRPLEATTEWQEYLETYADQGAIAL
                     ENSQLVRSLERTNQELQRAYDRTIEGWASALDLKDEETAGHSKRVTEMAVKLARKLGV
                     PTHELVHLQRGALLHDIGKMGIPDHILLKRGPLDAEEFEVMRQHPVYAYNLLFPIEFL
                     RPALDIPYCHHEKWDGTGYPRNLQGEQIPLAARIFAIVDVYDALTSDRPYRDAWPIER
                     ALEHIRAQSGTHFDPRVVQAFFDLLENEVAAEQHV"
     gene            2912..3502
                     /locus_tag="PKN52_00945"
     CDS             2912..3502
                     /locus_tag="PKN52_00945"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HNQ98531.1"
                     /translation="MSRTVKVLWIVAALAVVGAAAYYWGAVRPRPAALAGTMLQNPVP
                     VTGLRLIDTAGHEVDLADDFAGSVTLVFFGYTRCPDVCPLTMARLTRAYEILGAPTGL
                     KVVMVSVDPGHDTPEVMAEYLSRYHPDFVGLTGSNSQVAEAARTFFVGYAGSGATVAH
                     TDVVGVLDRHGSLRYIYGQDVVVKLGDDIPELLREL"
     gene            complement(3587..4369)
                     /locus_tag="PKN52_00950"
     CDS             complement(3587..4369)
                     /locus_tag="PKN52_00950"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNQ98532.1"
                     /translation="MTAAADASHLRTIHPMVRGGIADRHAALHNGAPPSPQAETLTDA
                     TAVLVIPGLHEVASSWPVRTYAKGDRLYQVDAPATTLFYLEAGLVALELNTPKPRIGA
                     LAGPGDMVGALATGCGTYLESAVALSGEVAVRVLDERAQAELPAPDLATLVAAAAGAR
                     LAALTQALEDGEHPVPARLARAFLRLGSRFGQTLADGAVRLTLPVTHDTLAAMVGAAR
                     ETTTAVVADMRRLGLVEGTRGSYRLRPAELMDYALEATLSAQ"
     gene            complement(4423..>5449)
                     /locus_tag="PKN52_00955"
     CDS             complement(4423..>5449)
                     /locus_tag="PKN52_00955"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HNQ98533.1"
                     /translation="VDDPGLVADLIRRAELIGQARLRPAATVSVGMRGDRLTDFAALK
                     AAGAVMLTDDGLPLADAHLMRRACEYAADLGLVIQTHSEEPRLRADGVMNEGSVSREL
                     GLPGNTASAEAIMVFRDAEIARLTGARVHIAHVSCERAMRVAQWQRANGAPITIEVTP
                     QHLTLTDKSLRAFDPVFKVAPPLRTAADVACLREALRLGAVDNIGTDHAPHTLAEKER
                     DLLEAPFGIANLEVTFALLYTRLVLTGELQLVELLRLLQAGPASVMGWSAPTLAPGAP
                     ADLTVVDLDSELPVRGRSFRSKAKHNPWEGEPLRGWPRLTLVRGNLCFDREAVLPTSH
                     RELTKGE"
BASE COUNT          824 a         1855 c         1927 g          843 t
ORIGIN      
        1 ggggcgcatc ccacccttcg agctcgacga gtcgatcacc taccacgacc cctgctacct
       61 cgggcgccac aacggcatct acgacgcgcc gcgccaggtg ctcgagagcg gcggcgccac
      121 gctcctcgag atggagcgca accgcaacga ctccttctgc tgcggcgcag gcggagcgca
      181 gttctggaag gaagaggagg aaggcgacgg tcgcgtctcg aacgagcgct tcgaggaggc
      241 cgtcgcgacc ggcgcccagg tcatcgcgac cggctgcccg ttctgcaagg tgatgctctc
      301 gtcgtcggac ggggcggagg gagagaccgc gccgcaggtc atggacgtcg ctcagctggt
      361 cgccatgagg ctcgacaaca tccaggcgct cttcgaccat cacgccggcg cgggtggagc
      421 ggtcggagga agcgtaagcg gcgactgaca cggcgaaacc cgcgtgagtg gtatcttcgt
      481 caacgcttcc tcggccactg cgcatatcct tggggggcac gtgatcatgc gctatggtca
      541 cgccgggtcg ccagggaggc aacgaccgaa gtgtcgatgg accggttggg ctacaccgtg
      601 aaggcgtcgc tgggcgcgga cggcagcgag tcggatggcg gtgctgccaa ccagcagcgg
      661 gccgggctcg ggaccatgtt gctggccgag gtgttcgcgc gcgtcaagga cgcgctgatc
      721 gtcctcgacc aggccgacgt cgtcgtggcc gccaaccccg ccgccgaagc gctcatgcag
      781 gtcaggcagg gcggactcgt tggcatgccg ctcgtcaagc tcctgccggt ctccgcgccc
      841 ctcctggcgc tcgtggtcgc cccggatcgt cgaggctccc tcgaactgga ggtcgacctg
      901 gtcaggccgc agggcggcct ggtgccagcc tccgtcagcg ccttccggct ggacgacggc
      961 cgcaccgtac tcgtctgcca ctcgccaagc ataggtgtgc tcgactcccg ctcgccccgc
     1021 gccgagcggc ggttccgccg catggccgag agctcccctg acgtccggtt cgtcatcgac
     1081 gcgcacgagc gcatcgagta cgcctcgccg tccgccgccg aactcggcct ggtggccggc
     1141 gagcatacgg aggccgacgt gtggcgcctc gtgcatccgc gcgaccgggc cgccgcccaa
     1201 cgggccctgg cggcgcgtta cgaccggtcg gccgagccgc tcgaggacgt cgtggtgacg
     1261 gtccgcgcgc gcgtcggcga cggttggcgg tggttcgagg cgcgcgtcac cgacctcacg
     1321 gacgacccgg acatccgcgg cctgctggtg caggcgcgcg acgtcaccga cagggtggag
     1381 gagctgaagc gcctgcgcgc cagcgaggct tacctgcgcg ccatcttcga gcgcaaccac
     1441 gacggcctcg tgctgcttga caagcgcaag cacatcgtca ggctggtggg ccggtcgcag
     1501 gaagacctcg gctactccgc gtccgatctg ctcggctcac gcaacgtcca cctggtccat
     1561 cccgacgaca gggaggcgct ctcggacgcg ttcgacatgg tcgaggcgcg cctgggcgcg
     1621 acggagcagg tcaggttgag agtgagggac cgggcgggag cctaccactg gatcgagggg
     1681 tcgctctcga acatgctcca cgatccggac gtgcgcgcgt tcgtcctgtc ctatagaagc
     1741 gtcggcgatc atggtccgac cgtcgacagg gtcaggtcat tgaacgacca gttgacgcgg
     1801 cgggtccagc acctccagtc gctcaggcgc atcgacatgg ccatcaacaa cagtgccgac
     1861 ctgcggctcg tgttcgagat cttcatgaac cagctcgtgc aggacctggg cgtcgacgcc
     1921 gtggccgtgc tcctctacga gcctcagctc cagcagctca ggccgttcat gggcctcggc
     1981 ttcctgagcg agctacgcgt gcgcacgcgc gtcccgctcg gagagggccc ggcgggcagg
     2041 gccgcggccg atcagcacaa cgtgtacgtg cccgacctgc ggctcggcag cgtgtggggc
     2101 gccgccatcg agccggagca ccagaccggg ttctcctcgt acatggcggt ccctatgacc
     2161 gccaaggggc agctgcacgg cgtcctcgag ctgtacgcgc ggcggccgct cgaggccacg
     2221 accgagtggc aggagtacct ggagacgtat gccgaccagg gcgccatagc gttggagaac
     2281 tcgcagctcg tgcgctcgct cgagcggacc aaccaggagt tgcaacgcgc gtacgatcgc
     2341 acgatcgaag gctgggcgag cgccctcgac ctcaaggacg aggagaccgc cggccatagc
     2401 aagcgcgtca cggagatggc cgtgaagctg gctcgcaagc tgggcgtgcc gacgcacgag
     2461 ctggtacacc tccagcgcgg tgcgctcctg cacgacatcg gcaagatggg gatacccgac
     2521 cacatcctcc tcaagcgcgg cccgctcgac gccgaggagt tcgaggtcat gcgccaacac
     2581 cccgtctacg cttacaacct gctcttcccg atcgagttct tgcgtccggc gctcgacatc
     2641 ccttactgcc atcacgagaa gtgggacggc acggggtacc cgcgcaacct acaaggggag
     2701 cagatcccgc tcgcggccag gatcttcgcc atcgtcgacg tctacgacgc cctgacctcg
     2761 gaccgcccct accgcgacgc ctggccgatc gagcgcgcgc tggaacacat cagggcccag
     2821 agcggcacgc acttcgaccc gcgggtcgtt caggcgttct tcgacctgtt ggagaacgag
     2881 gtcgccgcgg aacaacacgt atagtcgccg ggtgagtcgc accgtcaaag tcttgtggat
     2941 cgtcgccgcg ctggccgtgg tcggcgcggc ggcctactac tggggcgcgg tccggccgcg
     3001 gccggccgcg ttggcgggca ccatgctcca gaacccggtg ccagtgacgg gactgcgcct
     3061 gatcgatact gccggacacg aggtcgacct ggccgacgac ttcgcaggct cggtgaccct
     3121 cgtgttcttc ggttacacgc gctgcccgga cgtctgcccg ctgaccatgg cccgcctcac
     3181 gcgcgcctac gagatcctcg gggcgcctac agggctgaag gtcgtgatgg tgtcggtcga
     3241 ccctggtcat gacacgcccg aggtcatggc ggagtacctg agccgctacc acccggactt
     3301 cgtcgggctg accggctcga actcccaggt ggcggaggcg gcccggacgt tcttcgtcgg
     3361 ctacgccggt tccggcgcca ccgtggcgca caccgacgtg gtcggagtgc tggaccgaca
     3421 cggctctttg cgctacatct acggccagga cgtggtcgtg aagctgggag acgacatccc
     3481 cgaactgttg cgcgaactct gacggtcatc gaccgacgct gctgcggcgg ggtacggaag
     3541 tccccgctgt gccgtcggaa cgccttgcgc ttgtgttgtg ggatccttac tgcgcgctga
     3601 gcgtggcttc cagcgcgtaa tccatgagtt cggccgggcg caggcgatag cttccgcgcg
     3661 tgccctcgac gaggccgagc cgtcgcatgt cggcgaccac cgccgtggtg gtctcgcgcg
     3721 ccgcgccgac catggcggcg agcgtgtcgt gcgtgacggg cagggtcagg cgcacggcgc
     3781 catcggccaa ggtctggccg aagcgcgagc cgaggcgcag gaacgcgcgg gccaggcggg
     3841 ccggcacggg gtgctccccg tcttccaacg cctgcgtgag cgcggccagg cgcgcgccgg
     3901 ctgccgccgc gaccagcgtg gccaggtcgg gagcgggcaa ctcggcctgg gcgcgctcgt
     3961 cgagcactct cacggcgacc tcgccgctga gcgcgaccgc gctctccaag tacgttccgc
     4021 aaccggtggc gagggcgcct accatgtcgc cagggccggc cagtgcccct atgcggggct
     4081 tgggcgtgtt cagttccagg gccaccagcc cggcctccag gtagaagagc gtggtagccg
     4141 gcgcatcgac ctggtagagc ctgtcgccct tggcgtaagt cctgaccggc caactcgagg
     4201 ccacctcatg caggccgggg atgacgagaa ccgcggttgc gtcggtgagc gtttccgcct
     4261 gtggggaggg cggcgcgccg ttatgcagcg cggcatgccg gtctgcgatg ccgccgcgca
     4321 ccatggggtg gatggtacgc aggtgtgagg cgtccgctgc tgccgtcatg gggacatcat
     4381 agtggttcga gtcgctgatt ccgtgatttc ctaggcattt acctactcac ccttggttaa
     4441 ctccctgtga ctcgtgggca ggacggcctc gcggtcgaag cagaggttcc cgcgcacgag
     4501 cgtcaggcgc ggccaaccgc gtaacggctc gccttcccac gggttgtgct tcgccttgct
     4561 cctgaacgac cgacccctca cgggcagctc cgagtcgagg tcgacgaccg tgaggtcggc
     4621 gggcgcgccg ggcgccagcg tcggggcgga ccagcccatc accgaggcgg ggccggcctg
     4681 aagcaggcgc aggagctcga cgagctggag ctcgccggtc aggacgagtc gcgtgtagag
     4741 cagggcgaag gtcacctcga ggttggcgat gccgaacggc gcctctagga ggtcccgctc
     4801 cttctccgcc agcgtgtggg gggcgtggtc cgtaccgatg ttgtcgaccg cgcctagccg
     4861 aagcgcttcc cgtaggcagg ccacatccgc cgcggtcctg aggggagggg cgaccttgaa
     4921 caccgggtcg aacgcgcgca aactcttgtc ggtcagggtc agatgctggg gcgtgacctc
     4981 gatggtgatc ggcgcgccgt tggcgcgctg ccactgcgcg acccgcatgg cgcgctcgca
     5041 gctcacgtgg gcgatgtgca cgcgcgcgcc cgtcagtcgc gcgatctcgg cgtcgcgaaa
     5101 gaccatgatg gcctcggcgg acgcggtgtt gccgggtagt ccgagctcgc ggctcacgct
     5161 gccctcgttc atgacgccgt ccgcccgcaa ccgcggctcc tcggagtgcg tctggatgac
     5221 gaggccgagg tcggcagcgt actcgcaggc ccggcgcatg aggtgggcgt ctgcgagggg
     5281 caagccgtcg tccgtgagca tgacggcgcc ggccgccttg agggcggcga agtcggtcag
     5341 gcggtcgccg cgcatcccaa cgctcacggt cgccgccggc cgtaggcgcg cttggccgat
     5401 gagttcggcc cggcggatca ggtcggcgac gaggccgggg tcgtcgacg
//
LOCUS       DAOQYE010000024         5444 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_15296_length_5444_cov_3.022824, whole genome shotgun sequence.
ACCESSION   DAOQYE010000024 DAOQYE010000000
VERSION     DAOQYE010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 5444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5444
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15296_length_5444_cov_3.022824"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..678)
                     /locus_tag="PKN52_00960"
     CDS             complement(<1..678)
                     /locus_tag="PKN52_00960"
                     /inference="COORDINATES: protein motif:HMM:NF018218.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HNQ98534.1"
                     /translation="MRRSFNPWLVVMLLILGIFVFNQLNSGSGSREINYSTFTGLVDQ
                     GKVASVVIDRNSGVISGELVSESQVLVNGEPQTIRSFRTTTILTDSLLTELRNDVGSV
                     TIRNPPQWLGLLLGSFLPVVLLVVFFWFIFMRAQGGPNQVMQFGQSRAKTFGRENKVT
                     TTFNDVAGHKEAKQELIEVVDFLKTPQKYLRIGAEIPKGVLLVGPPGTGKTLLARAVA
                     GEAGVPFL"
     gene            complement(892..2145)
                     /gene="glgC"
                     /locus_tag="PKN52_00965"
     CDS             complement(892..2145)
                     /gene="glgC"
                     /locus_tag="PKN52_00965"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HNQ98535.1"
                     /translation="MLTRNVKVIGMVLAGGKGSRLHPLTLQRTKPAVPFGSKYRIIDF
                     ALNNMINSGVYGMYILTQFKAQSLTEHIQRHWRFGTFLDDHFITLAPAQMYLYEELGA
                     EWYRGTADAIYQNLHLLGNNRADLVAIFSGDHIYKMDISHMIEYHLDNQADVTIAAYP
                     TPVEDGKRFGVLQVDNDWRITEFQEKPAAPKAIPGRPTHCLASMGNYVFTVSTLIEML
                     KDDVGRRDSEHDFGKDVLPRALQEGKRMFAYDFARNPIPGQVGPNTYWRDVGTLDSYW
                     EANMDLVAVKPEFDLYNEEWPLRTSAEYSAPAKFVHEEEGRRGQAFNSLVAGGVIISG
                     ATVRHSVVFRRVRVNSYATVENSVLFDNVTVGRHARVVNAILDKNVTVPDGVTVGVDP
                     EADRARGLVVTDSGLVCVPKGYKFS"
     gene            2265..3368
                     /gene="rlmN"
                     /locus_tag="PKN52_00970"
     CDS             2265..3368
                     /gene="rlmN"
                     /locus_tag="PKN52_00970"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HNQ98536.1"
                     /translation="MAALAPTLFDHDLASLPLPEGEPAFRRRQVANWLYRRGVTDAAQ
                     MTDLPAPLRAWLAAEQAAMPFVASRRFPSDDGSVRYLFTLRDGKQTEAVYMPYAGRRT
                     VCLSSMVGCPAGCAFCATGALGFGRNLTRGEIVGQLVEVARQEGLEPTEVRNVVLMGM
                     GEALLNYDNAIGAIRTFIDPDGLDMSPRRITLSTVGLPSRMRKLASEGLPLVLAVSLH
                     APDQETRQRIIPTALANSVTDIVAAMHDWQRATSRRVTIEYTMLEGVNDHPWQADMLA
                     ELLVGLTSHVNLIPFNPWTGSGFSGTQRPRLAQFQRRLEARGVGVSVRFSRGRDAGAA
                     CGQLALQGAEAASVADSGPVLSMADPGLAASDA"
     gene            3564..5093
                     /locus_tag="PKN52_00975"
     CDS             3564..5093
                     /locus_tag="PKN52_00975"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNQ98537.1"
                     /translation="MERGTRRGGGRAVLVAVAALLLLSPAWAQSVSELRAEVNSDPGS
                     VSAWVALGNAYYGQGEFALAKESYLEAIALDYTSGDAHYGLGLAEFSRGDYQAALFEF
                     TEVTRLYPERFDGQFNRAVTLARLRRPAEAATAFREAIAQAEPEASMDDRVQAQLGLA
                     AQLELSEDWSGAAAAYAAALELQPGTPAYILRRGQALYRAGRGLEALPELTALEAQSS
                     DYRVSALISDIYVAQGQIDYALWSLERALQKAERAEDKPAQASTLVKLGTLQRGLGRD
                     EQAHDSFERAALVYPASWEAQYNLGIANLEGGQTRDAVRALQDAQRLDGNRAEVYLAL
                     ATAHDQLSNSAEALSNAREAIARLSDPKLVLDARLIAGRAMYRLGDYLGAQAELQAVL
                     EDRPNDAQVQLWYGLAAYQLADYTQAVRNFERAVQLDPSSLVARVNLGAGYLAARRYE
                     DAEKVYQLLVGQNPNDSESLYNLGWALYSQDRRGSARDAWVSSCQQGYQPACTAISSY
                     L"
     gene            5208..>5444
                     /locus_tag="PKN52_00980"
     CDS             5208..>5444
                     /locus_tag="PKN52_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98538.1"
                     /translation="MDWLRRNWPDLLIGVALVAVIAGIIATLISGGSFFPIGPGNRNA
                     GGATTTQSSGTTPGTSGTTVTPTTPSGATAASGAT"
BASE COUNT          862 a         1858 c         1866 g          858 t
ORIGIN      
        1 gaggaacggc acgccggcct cgccggccac cgcgcgcgcg aggagcgtct tgccggtacc
       61 cggggggccc accaggagca cgcccttggg gatctcggcg ccgatgcgca ggtacttctg
      121 gggcgtcttc aggaagtcga cgacctcgat cagctcctgc ttcgcctcct tgtggccagc
      181 cacgtcgttg aaggtcgtcg tgaccttgtt ctcgcgcccg aacgtcttgg cgcgcgactg
      241 cccgaactgc atcacctggt tcggcccgcc ctgggcgcgc atgaagatga accagaagaa
      301 gacgacgagc agcacgacgg gcaggaagga accgagcaac agccccagcc actgcggcgg
      361 gttgcggatc gtcacgctgc cgacgtcgtt gcgcagctcc gtgaggaggc tgtcggtgag
      421 gatcgtcgtg gtgcggaagc tacggatggt ctggggctcg ccgttgacga gcacttgcga
      481 ctcgctgacg agctcgccgc tgatcacgcc gctgttacgg tcgatgacga ccgacgccac
      541 cttgccctga tcgacgaggc cggtgaacgt cgagtagttg atctcccggc tacccgagcc
      601 ggagttgagc tggttgaaca cgaagatgcc gagtatcagc agcatcacga ccagccaggg
      661 gttgaacgag cgtctcaact gattcctcca aggcccgggc tccgctccgg ccacacgcgg
      721 ttatggcaag cggcggtcgt ggggtgccgc gaaccgatgc gtaaagcgta gcaccgcacc
      781 atgagccggc actgtactgg ggagtcctta actaagcgga accttaacgt tccgccggcc
      841 ggcttcgcgt gcgacgaccg gtgcccggcg cgcgagcgag ggcggcacgg ttcagctgaa
      901 cttgtacccc ttcgggacgc agaccagccc gctgtcggtg accaccagac cgcgtgcccg
      961 gtccgcctcc gggtccaccc cgaccgtcac gccgtccggg acggtgacgt tcttatcgag
     1021 gatggcgttg acgacgcgcg cgtggcgtcc gacggtgacg ttgtcgaaga gcacggagtt
     1081 ctcgaccgtg gcgtaggagt tgacgcgcac cctgcggaag acgacgctat ggcgcaccgt
     1141 tgccccggag atgatgacgc cgccggccac cagggagttg aaagcctggc cgcgccgccc
     1201 ctcctcctcg tggacgaact tggcgggcgc cgagtactcg gccgacgtgc gcaggggcca
     1261 ctcctcgttg tagaggtcga actcgggctt gacggccacg aggtccatgt tcgcctccca
     1321 gtagctgtcg agcgtgccga cgtcgcgcca gtacgtgttg gggccgacct ggccggggat
     1381 cgggttgcga gcgaagtcgt acgcgaacat ccgcttgcct tcctgcaggg cccgcgggag
     1441 cacgtccttg ccgaagtcgt gctcggagtc gcggcggccg acgtcgtcct tgagcatctc
     1501 gatgagcgtg gagacggtga agacgtagtt gcccatgctg gcgaggcagt gcgtcggtcg
     1561 gcccgggatg gccttgggcg cggccggctt ctcctggaac tccgttatgc gccagtcgtt
     1621 gtccacctgc agcacgccga agcgcttgcc gtcctcgacg ggcgtcgggt aggcggcgat
     1681 ggtcacgtcg gcctggttgt cgaggtggta ctcgatcatg tggctgatgt ccatcttgta
     1741 gatgtggtcg ccggagaaga tcgcgacgag gtcggcgcgg ttgttgccta ggaggtgaag
     1801 gttctggtag atggcgtcgg cggtgccccg gtaccactcg gcgcccagct cctcgtacag
     1861 gtacatctgc gccggcgcga gcgtgatgaa gtggtcgtcg aggaacgtgc cgaagcgcca
     1921 gtgccgctgg atgtgctccg tcagcgactg ggccttgaac tgggtgagga tgtacatgcc
     1981 gtagacgccc gagttgatca tgttgttgag ggcgaagtcg atgatccggt acttggagcc
     2041 gaacggcacg gcgggcttcg tgcgctgcag cgtgagcggg tgcaggcgcg agcccttgcc
     2101 gccagccaac accatgccta tgaccttgac gttgcgggtg agcatgtggc ctccatggga
     2161 ccaagacccg accacggcgt cttgcccgga tcggctacca ccgccgatcg tccgtgtaag
     2221 tgctaagcat gatagcgccc ccgcggacgc ggtagcgtgg ccctatggcg gcgttggccc
     2281 caaccctgtt cgaccacgac ctggcctccc tgccgttgcc ggagggcgag cccgcgttcc
     2341 gtcgcaggca ggtcgccaac tggctctacc ggcgtggggt gacggacgcc gcccagatga
     2401 ccgaccttcc cgcgcccttg cgtgcgtggc tggcggccga gcaggcggcc atgccgttcg
     2461 tcgcctccag gcgtttcccc tcggacgacg gctcggtgcg ctacctcttc accttgcgcg
     2521 acggcaagca gactgaagcc gtgtacatgc cgtacgccgg acggcgcacc gtatgtctca
     2581 gctccatggt cgggtgcccg gccggctgcg cgttctgcgc caccggcgcg ctcggcttcg
     2641 ggcgcaacct cacgcgcggc gagatcgtcg gtcagctggt tgaggtggcg cgccaggagg
     2701 gcctggagcc gaccgaggtc cgcaacgtcg tcctcatggg catgggcgag gcgctgctca
     2761 actacgacaa cgccatcggc gccatccgta ccttcataga ccctgacggc ctcgacatgt
     2821 cgccgcgtcg cataacgctg tcgacggtcg ggctacccag ccgcatgcgg aagctcgcgt
     2881 cggaagggtt gccgctcgtg ctcgcggtca gcctccacgc gcccgaccag gagacgcggc
     2941 agcggataat cccgacggcc ctggccaact cggtgaccga catcgtcgcc gctatgcacg
     3001 actggcaacg ggccacctcc cgccgagtga ccatcgagta cacgatgctg gaaggcgtca
     3061 acgaccatcc gtggcaggcg gacatgctcg cggagctgct cgtcggcctc acgagtcatg
     3121 tgaacctcat cccgttcaac ccctggaccg gctcgggttt cagcggcacg cagcggccgc
     3181 gccttgcgca gttccagcgc cgtctcgagg cccgcggcgt gggcgtgtcg gtgcggttca
     3241 gtcgtggccg cgacgccgga gcggcgtgcg gccaactcgc actgcaaggc gccgaggccg
     3301 cgagcgtcgc cgactctggc ccggtgctga gcatggcaga ccccggcctg gcggcgagcg
     3361 acgcgtgagc ccccgggcgc cccacctgtc ggggcgcttg gcacgaagcg cgacgccgtc
     3421 tgcgccgacc gcccttgcgg cttggcggcg ttcgcgccgc cgcgtcaggc aggtggacga
     3481 tttcacaagc gcgcaagatg gcagcgtata agcttgcccc tgactaacgt ttggcacgcg
     3541 agttagcgac ctgggaggct gggatggaaa gaggaacccg cagggggggc ggccgcgcgg
     3601 tactggtcgc agtggccgcg ttgctcctgc tttcgccggc gtgggcacag tccgtctccg
     3661 agttgcgtgc cgaggtcaac tccgacccgg ggagcgtgag cgcttgggtg gcgctcggca
     3721 acgcttatta cggtcaggga gagttcgccc tcgccaagga gagctacctg gaggccatcg
     3781 ccctcgacta cacgtccggc gacgcccact acggcctcgg tctggcggag ttctcgcgcg
     3841 gcgactacca ggccgcgctc ttcgagttca cggaggtcac gcgcctctac cccgagcgtt
     3901 tcgacggtca gttcaaccgc gccgtcacgc tcgcccgcct gcggcgtccc gccgaggcgg
     3961 ccacggcgtt ccgcgaggcc atcgcccagg ccgagccaga ggcaagcatg gacgaccgcg
     4021 tgcaggccca actgggtctc gccgcccagc tggaactgag cgaagactgg tcgggcgccg
     4081 cggcggcgta cgccgccgcc ttggagctcc agcccggaac cccggcgtac atcctcaggc
     4141 gcggccaggc cctctaccgg gccgggcgcg gcctcgaggc gttgcccgaa ctgacggcgc
     4201 tcgaggcgca gagcagcgac taccgcgtca gcgccctcat ctccgacatc tacgtcgccc
     4261 aggggcagat cgactacgcc ctatggtcgc tagagcgcgc gctgcagaag gcggagcggg
     4321 ccgaggacaa gcccgcccag gcgagcacgc tcgtcaagct cggcaccctg caacgcggcc
     4381 tcggccgcga cgagcaggcc cacgactcct tcgagcgcgc cgccctcgtc tacccggcgt
     4441 cgtgggaggc gcagtacaac ctcggtatcg ccaacctgga gggcgggcag acccgagacg
     4501 cggtgcgtgc cctccaagac gcgcaacgcc tcgacggcaa ccgcgccgag gtctacttgg
     4561 cgctggccac cgcgcacgac caactctcca acagcgccga ggccctcagc aacgcccgtg
     4621 aggccatcgc gcgcctgtcc gacccgaagc tcgtgctcga cgcgcgcctc atcgcgggca
     4681 gggccatgta ccgtctcggc gactacctgg gtgcccaggc cgagctccag gccgtgctcg
     4741 aggaccgccc gaacgacgcc caggtacagc tctggtacgg gctcgccgcc taccagctcg
     4801 ccgactacac gcaggcagtg cgcaacttcg agcgggccgt gcagctggat ccgagcagtc
     4861 tggtggcgcg cgtcaacctc ggagccggtt acctcgcggc ccgtcggtac gaggacgccg
     4921 agaaggtgta ccagctcctg gtcggccaga acccgaacga cagcgagagc ctctacaacc
     4981 tcggttgggc cttgtactcg caggaccggc gcggctcggc ccgcgacgcg tgggtctcct
     5041 cgtgccagca gggctaccag ccggcttgca ccgccatcag cagctacctg tgaggcggat
     5101 tcatcggtga ggcgggtcgg tagcgaagcc gcggcacgcg gccgctagcg acccaaggga
     5161 actctaggca gcgccaccgc gctgaacgac gaaggtacaa tgacgctatg gattggcttc
     5221 gacgcaactg gcccgatctc ctgataggcg tggccctggt ggccgtcatc gccggcatca
     5281 tcgcaacgtt gatatccgga gggtcgttct tcccgatcgg gccgggcaac cgcaacgcgg
     5341 gcggagccac gaccacgcag agttccggca cgacgcccgg aacgtccggc accacggtga
     5401 cgccgaccac tccgtccgga gcgaccgctg catctggagc gacc
//
LOCUS       DAOQYE010000025         5340 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_15693_length_5340_cov_3.185430, whole genome shotgun sequence.
ACCESSION   DAOQYE010000025 DAOQYE010000000
VERSION     DAOQYE010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 5340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5340
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15693_length_5340_cov_3.185430"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..873
                     /locus_tag="PKN52_00985"
     CDS             <1..873
                     /locus_tag="PKN52_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98539.1"
                     /translation="LPGELPRHRPPGGMMGGARRGGFDTDWRGVASRTTVKLLAMTLC
                     ALVALWLGAVAAAQDGSADAEQAIAIAARHPMFATGLAAYDGWTASAYGSQDRYGLWR
                     VDFFAADGASLGWAQVGLDDERVFAWDSGFGIPDVSYEATQEALLDFLRYDSEFLAFA
                     GDVDDNDWTWVGYDDSRDAWTVYLERGPESLVVTLRSANEWARSLDNLRVVEIEVPAL
                     VAVDEWRSMRGSAATALAFTDARIAAAVRGLEGWVSEAEQLGESVWLVRFRWEGRTVA
                     EAEVDLVSASVAVR"
     gene            1064..1456
                     /gene="trxA"
                     /locus_tag="PKN52_00990"
     CDS             1064..1456
                     /gene="trxA"
                     /locus_tag="PKN52_00990"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNQ98540.1"
                     /translation="MASKEITATNFNETIADNDIVFLDFWAAWCGPCRMFAPVFEKAS
                     EDHPDVVFGKVDTESQQELAGAFRIMSIPTLMAFRENVLVFAQPGALNGAQLEQVIAA
                     VRGLNMEEVHAEVAAQAEKSEAAEGATA"
     gene            1453..1836
                     /locus_tag="PKN52_00995"
     CDS             1453..1836
                     /locus_tag="PKN52_00995"
                     /inference="COORDINATES: protein motif:HMM:NF014624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HNQ98541.1"
                     /translation="MSTAEVDGQGIAAVTVEGERQYDPVGASDVGAQASPGSSTDQEA
                     RRRVLHRLRRAQGQLAAVVEAVDEGKPCRDVVTQLAAVSKALDRAGVVLISNAMQECL
                     ADAEAPDRADKLTLDEVEKLFLMLA"
     gene            complement(1921..2919)
                     /locus_tag="PKN52_01000"
     CDS             complement(1921..2919)
                     /locus_tag="PKN52_01000"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNQ98542.1"
                     /translation="MNKLCVTLLLCVSLCAATAFAQGVFSEAPGRSVSVDGRSLYLYC
                     TGEGSPTVLFEAGLGDGSINFRALQARIAPFTRACAYDRAGYGLSDAGPEPRDLTASV
                     SDLEKVLAATGEDGPYVLVGHSYGGLIALSFANHNRGSVRGVVLVDSSHPEQLTALQS
                     VPEVVTVQDMEIAGLADLVGAAEAGALPAEAVLPSAPSGLPAALQQAWAEFFVSPKQL
                     KATVAEYDSLEASLAQAAARFDLGNTPLIVLSRGIGLEGQLPAEALDVLGLTPDVLAR
                     FNAVWNDLQLDLTTVSTSSKRVVAERSTHYVYQDQPALVEAAIRELVRKARTPSPN"
     gene            3059..3472
                     /locus_tag="PKN52_01005"
     CDS             3059..3472
                     /locus_tag="PKN52_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="group III truncated hemoglobin"
                     /protein_id="HNQ98543.1"
                     /translation="MSEVAFAPTNEPRGELHSKVDDENIAILVERFYAAVQKHPTLGP
                     VFNPRLEGRWDRHMSQMKDFWSSVLLRSGRYSGYPLRAHFGVPGMAPERFDDWLVLFR
                     QTLDGLYEPPYADAIYAMAQQFAQRFTGALFDQGR"
     gene            complement(3607..4647)
                     /locus_tag="PKN52_01010"
     CDS             complement(3607..4647)
                     /locus_tag="PKN52_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98544.1"
                     /translation="MRRFLRRLLGVALVAVALALVVGKLWLASGGPYLEALPMYPYCA
                     ESSAALAEGRHQDALELAEAGGCERESAAAQAEWNRFGAVVGRCAQGIWTGRGEDGYG
                     IGCAMASDLVVVGDVRDLVRQGVIWIQGGQTDPVLIALSAAGVALTFTPQIGAGASLF
                     KSARRAGSVGEPLAKSVAKLAGEQAWKPLAGMLGDAGRIGRKLPIGRATKALAYADDA
                     DDLAAIARFVEVAPNPLLGLKWGGKGVTRLVDEGLYAQAALRGPAGIRLAVERGGKAL
                     LTRQPLMVFAAKTLYVNGDRLLPLVLGLLTWPWVWGVAVALALVGLVFVVWGRRGPRR
                     NARTRAPTGVPT"
     gene            4725..>5340
                     /locus_tag="PKN52_01015"
     CDS             4725..>5340
                     /locus_tag="PKN52_01015"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HNQ98545.1"
                     /translation="MIDIPDLGLYDAVLFDLDGVLTPTAEVHRAAWRELFAPYLATRG
                     AAPYQESDYFEHLDGRQRYDGVAALLASRGLSLPRGTPEDPPEAETVCGLGNRKNAVF
                     QRVLDEEGVAPCAGAVALVDALCAAGVTVAVVSGSRNARTVLAAAGLAERFPLVVGGI
                     EAAELSLASKPAPDAFLHAAAVLGVEPARAVVIEDAISGVAAGKA"
BASE COUNT          844 a         1817 c         1874 g          805 t
ORIGIN      
        1 ctaccgggcg aactaccgcg gcatcgtccg cccgggggca tgatgggcgg cgctcggcgc
       61 ggcgggttcg acacagactg gcgcggcgtt gcgtcgcgga cgaccgtcaa gctgctagcc
      121 atgaccctct gcgcgctggt cgcactgtgg ctcggtgcag tggccgccgc ccaggacggc
      181 agcgccgacg cggagcaggc catcgccatc gctgcccgcc atccgatgtt cgccaccgga
      241 ctggcggcct acgacgggtg gacggccagc gcttacggct cgcaggaccg ttacgggctc
      301 tggcgcgtcg acttcttcgc ggcggacggc gcctccctag gctgggcgca ggtggggctg
      361 gacgacgagc gcgtgttcgc ttgggactcc ggcttcggta tcccggacgt ctcctacgag
      421 gccacccagg aggcgctgct cgacttcctg cgctacgaca gcgagttcct cgcgttcgcc
      481 ggcgacgtgg acgacaacga ctggacttgg gtcggctacg acgactcgcg cgacgcgtgg
      541 accgtgtacc tggagcgcgg accggagtcg ctcgtcgtca ccttgcgttc ggccaacgag
      601 tgggcgcgct cgcttgacaa cctgcgggtc gtcgagatcg aagtgccggc gctcgtcgcc
      661 gtggacgagt ggcgctccat gcgcggctcg gccgcgacgg cgctagcgtt cacggacgcg
      721 cgcatcgcgg cggccgtacg cggactggaa ggctgggtct ccgaagccga gcaactcggc
      781 gagtccgtct ggctcgtgcg cttccgctgg gaagggcgga ccgtggcgga ggccgaagtg
      841 gacctggtgt cggcgtcggt ggccgtcagg tagcggcgac accgttcagc ggcacgctcg
      901 atcggcgtta ccggcaaccc gcctacgtcg atgccgacgt atcacctccg cttggtgcgc
      961 gggacacccg gtgagcatgg caacccggta acgggacttc gcatcacctc cgcccggcgt
     1021 gtaggacacg cggtggtcaa acccctaggg gtatgctgcg accatggctt cgaaagagat
     1081 caccgctacc aacttcaacg agaccatcgc cgacaacgac atcgtcttcc tcgacttctg
     1141 ggccgcctgg tgcgggccgt gccggatgtt cgcgcccgtg ttcgagaagg cctcggagga
     1201 ccacccggac gtggtgttcg gcaaggtaga caccgagtct cagcaggagc tcgcgggcgc
     1261 ctttcgcatc atgtccatcc cgaccctgat ggccttcaga gagaacgtgc tcgttttcgc
     1321 gcagcctggc gcgctgaacg gcgcgcagct cgagcaggtc atcgcggccg tgcgtggtct
     1381 gaacatggag gaagtccacg cggaggtcgc cgcccaagcc gagaagtcgg aggcggccga
     1441 aggggcaact gcgtgagcac ggccgaagtg gacggccaag gcatcgccgc cgtcacagta
     1501 gaaggcgaac gacaatacga tcccgttggc gcgtcagatg tcggcgccca agcctcccca
     1561 gggagttcaa ccgaccagga agcgcgccgc cgtgtcctcc accggctaag gcgcgcgcag
     1621 gggcagttgg ccgccgtcgt agaggcggtc gacgagggca agccgtgccg cgacgtcgtg
     1681 actcagctcg ccgccgtgtc gaaggcgctt gaccgagccg gggtcgtgct catcagcaac
     1741 gccatgcagg agtgcctggc cgatgccgaa gctccggacc gcgcagacaa gctgacgctc
     1801 gacgaggtcg agaagctgtt cctgatgctg gcctgacgcc gccctcgggc cgacggtgtc
     1861 gaccatgtac agatgtgcga cggggctgct gccagcgcgc ttcggagcgt gcagctagag
     1921 tcagttgggg cttggagtgc gcgccttgcg cactagttct ctgatggccg cctccacgag
     1981 cgccggctgg tcctgataga cgtaatgggt gctacgttcg gcgaccacgc gcttcgaaga
     2041 ggtcgatacc gtagtcaggt cgagctgtag gtcgttccac accgcgttga agcgtgccag
     2101 cacatcgggt gtcagcccga ggacgtcgag cgcctcggcg ggcaattggc cctccaaccc
     2161 gatacctcgc gacaagacga tgagcggcgt gttcccgagg tcgaaccgtg cagccgcctg
     2221 agcgagggat gcctccaggc tgtcgtactc ggcgaccgtc gccttcagtt gttttggtga
     2281 gacgaagaac tcagcccacg cctgctgcag ggccgccggc agtccggacg gcgcgcttgg
     2341 gagcacggct tcagcgggga gcgcgccggc ctccgcagcg ccgacgaggt ccgccaaccc
     2401 ggcgatctcc atgtcctgca ccgttacgac ctcggggacg gactggaggg cggtcagctg
     2461 ctcagggtgc gaggagtcga ccagaactac gccgcggacc gagccgcggt tgtggttggc
     2521 gaagctcagg gcgatgagcc cgccgtaaga gtgcccgacc agaacgtacg ggccgtcctc
     2581 gcccgtggca gccaacacct tctcaaggtc ggataccgag gcggtcaggt cgcgcggttc
     2641 cggcccagca tcgctgagcc cgtacccggc ccggtcgtag gcgcaggcgc gagtgaacgg
     2701 cgcgatgcga gcctgcagcg cacggaagtt gatcgagccg tcaccgaggc cggcctcgaa
     2761 caagaccgtg ggactgcctt cgcccgtgca gtacaggtag agcgagcgcc cgtcgacgga
     2821 caccgaccgg ccgggcgctt cggagaagac gccttgggcg aaagccgttg cagcgcacaa
     2881 ggagacgcag aggaggaggg tgacgcagag cttgttcaag gtgttccctt cgtgcgattc
     2941 gggccgtcgg ccgctggtgg tcgcatggac ttgtcgcatc ttatccaggg ccgccggttg
     3001 tgatgacggc cgcggcgcgg ctgcgtccga gccgagcgac cggtgctacg ctcctgacat
     3061 gagcgaagtc gcctttgccc caactaatga gccaaggggc gaactacaca gcaaggtcga
     3121 cgacgagaac atcgccatcc tcgtcgagcg cttctacgcc gcggtgcaga agcacccgac
     3181 cctcggaccc gtcttcaacc cgcgcctcga ggggcggtgg gaccggcaca tgtctcagat
     3241 gaaggacttc tggtcgagcg tgctgctcag gagcgggcgc tacagcggtt acccgctgag
     3301 ggcgcacttc ggcgtgcccg gcatggcgcc cgagcgtttc gatgactggc tggtgctgtt
     3361 ccggcagacg ctcgatgggc tctacgaacc gccgtacgca gacgccatct acgccatggc
     3421 gcagcagttc gcgcaacggt tcaccggggc gctcttcgac caggggcggt agcgccaaga
     3481 gtcgggctgc gactcgcggc tgcctgcaat cgtgtttgcc tgcaatcgta gttgtatgaa
     3541 accgtgattg caggccgggg caagaccggc atacctcaag gtaaagacgg cggccgcaac
     3601 ggccgactac gtgggaacgc cggtaggcgc acgcgtccgt gcgttccgcc gcggcccccg
     3661 ccgtccccat accacgaaca ccaaccccac gagcgccaga gccaccgcta cgccccagac
     3721 ccaaggccac gtgagcagcc cgaggacgag cggcagtagg cggtcgccgt tgacgtagag
     3781 ggtcttggcc gcgaacacca tgagcggctg cctggtcaga agcgccttac cgccacgctc
     3841 aacggccagt cggatgccgg ccggaccccg cagcgccgct tgggcgtaga gcccctcgtc
     3901 caccaggcgc gtgacgccct tgccacccca cttgagcccg aggagcgggt tcggggccac
     3961 ctcgacgaag cgtgcgatcg cggctaggtc gtcggcatcg tcggcgtaag caagcgcctt
     4021 ggtcgcgcgc ccgatgggga gcttccgccc gatgcgcccg gcgtcgccga gcatgccggc
     4081 cagcggcttc cacgcctgct cgcccgccag cttcgcaacg ctcttggcga ggggctcgcc
     4141 gacggagccg gctcgcctgg ccgacttgaa gagcgaggcg cccgcgccga tctgcggagt
     4201 gaaggtaagc gccacccccg cggcggacaa ggctatgagc acggggtcgg tctgaccgcc
     4261 ctggatccag atgacgccct gcctcacgag gtcgcggacg tcgcccacta ccacgaggtc
     4321 gctcgccatg gcgcaaccga tgccgtagcc gtcctcgcca cggccggtcc agatgccctg
     4381 ggcgcaccgc cccaccacgg cgccgaatcg gttccactcc gcctgcgcgg ccgcgctctc
     4441 ccgctcacag ccgccggcct ctgccagctc gagcgcgtct tggtgacgcc cctccgcgag
     4501 cgcggcgctc gattcggcgc agtacgggta catggggagc gcctcgaggt acgggccgcc
     4561 gctggcgagc cagagcttgc cgacgacgag cgcgagggcc acggccacca gcgccacgcc
     4621 caggagtctc cgaaggaaac gccgcatcgc ttgagcgtac tgtgccgccg ggcggtgcca
     4681 cgtgagcgag tgtcgcggcg gcgactcgcg ctatccttcg gctcgtgatc gacatcccgg
     4741 acctgggtct ttacgacgcg gtcctgttcg acctcgacgg cgtgctcacg ccgaccgccg
     4801 aggtgcatag ggcagcgtgg cgcgaactgt tcgcgcccta cctcgcgacc cgcggcgcgg
     4861 cgccatatca ggagagcgac tacttcgagc acctggacgg ccgccagcgt tatgacggcg
     4921 tcgcggcgct gctcgcctcc agggggctga gcctgccgag gggtacgccc gaggacccgc
     4981 ccgaggccga gacggtatgc ggactcggga accgcaagaa cgccgtgttc cagcgggtgc
     5041 tcgatgagga gggcgtggcg ccctgcgccg gcgccgtggc gctggtcgac gcgctgtgcg
     5101 ccgccggcgt gacggtcgct gtcgtctccg ggtcgcggaa cgcgcggacg gtgctcgcgg
     5161 cggccggcct cgccgagcgc ttcccgctgg tggtgggcgg aatcgaggcg gccgagctct
     5221 cactcgcctc caagccggcg ccggacgcgt tcctgcacgc cgccgccgtt ctcggcgtcg
     5281 agccggcgcg cgccgtggtt atcgaggacg ccatctccgg cgtggcggca ggcaaggcag
//
LOCUS       DAOQYE010000026         5256 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_16004_length_5256_cov_3.095943, whole genome shotgun sequence.
ACCESSION   DAOQYE010000026 DAOQYE010000000
VERSION     DAOQYE010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 5256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5256
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16004_length_5256_cov_3.095943"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1049
                     /locus_tag="PKN52_01020"
     CDS             <1..1049
                     /locus_tag="PKN52_01020"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNQ98546.1"
                     /translation="AGVPTIVPTRVSAVRSEGSADVTISGASFVGAPASPALTLFDSP
                     RFTLSATTVVGFLDGIYLERSDSVRVVGNRFEQAARYALHVMFSNDVVLEGNTVRGGN
                     VGSAAMYGRGLRARGNVFAGHTGPLAFGLLIQELAEAEVSGNAFIGNTVGVLVVSAPD
                     VTISDNLLADNGFAMLVQRTRTGGTSAVRLQANSFEGNVSDVALDDPEAAVSVVGNHF
                     EGASRLDLDHDGVSDVPHLPSSSYDMFASRQPDLSLFALNPGILLWQTAEVTVPALRL
                     ATLTDAAPALRPSVGAAAAGVVAGKDTAGPATGAPVGREPTGIGTSTVMALGLVAACV
                     FLGAFLGRPVGESS"
     gene            1046..1837
                     /locus_tag="PKN52_01025"
     CDS             1046..1837
                     /locus_tag="PKN52_01025"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ98547.1"
                     /translation="MSPLLAERTEREPAGTGVHAVSVAALERRGRLDRVTFDLGAGAL
                     ALIGPNGAGKSTILHVLVGRIRSHGGAALLFGHKPRTVAAARLRAYVPQQIAFPPHLR
                     VREVLAAASKAKGCATERAEAAAARMGIDGFMSRRAGTLSGGMTQRLALAAALMDEPS
                     LWLLDEPASALDGGGLERLADWAREHVRAGGTLVVSAHRPEEVEAFASEAILLVRGRV
                     VGRVPVHELFDDRLVTTGGRAPLPEGTYVERSATPLLRRVLGGDE"
     gene            2275..2946
                     /locus_tag="PKN52_01030"
     CDS             2275..2946
                     /locus_tag="PKN52_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HNQ98548.1"
                     /translation="MTHDRRTFLKKLAVASVAAPLTLGLARAQMGHMGGGDTGMGGMG
                     GMMSSPSLGIGPDILAADEIPWDTGICAFCGMTIATPADGPNPAGFRERTYAQIRLAP
                     QHEGGMPSTLHYESLACMFNHAYTLGIADGHGATFYVADLSAPPRTAADLVLSRAATF
                     LWASELSVSMQARLGAFRDQAALHAYVSHEEVGRHHVLDAELLADLAPMPEAGLLGLL
                     TKHSE"
     gene            2949..3461
                     /locus_tag="PKN52_01035"
     CDS             2949..3461
                     /locus_tag="PKN52_01035"
                     /inference="COORDINATES: protein motif:HMM:NF017394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HNQ98549.1"
                     /translation="MAKGIDRRTFIRKTGHTLGAVLLWSQRAWADPRPVTFGVDVCPY
                     CNMTVVDLRFTAQVVTPTGLVHHYDAIECLADHLMGHGPRPPEVAETYLADRAVSSRD
                     EAAFLEVEEAVVLHHARLRTPMGGGLAAFADRAAAEEFAAAGRLAGAEYLTWREVLER
                     GRDRPWVPDY"
     gene            3475..4224
                     /locus_tag="PKN52_01040"
     CDS             3475..4224
                     /locus_tag="PKN52_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98550.1"
                     /translation="MAGVHLREVLRNPWTLFAGLALVGISLAFSGRGSAVGLVSLVAV
                     LQLYLPPLTVVVAAPLLTRRETWAFWAALPRPAGAAYRGATVGIAVGLLLPLLAGTGA
                     AAAILGLGALELLLLIATVAALVVLWTCTVSFLSALTLDPTRAMALGLAAWALLVLAY
                     GPTVVGLAVAFADYPLGPFMLLAIALNPTELLRVGLLQALGVPVLVGPVGKLVVDTVP
                     NGGFGLGVAAVLMLSALLLWLSGLWFARRDR"
     gene            complement(4294..4749)
                     /locus_tag="PKN52_01045"
     CDS             complement(4294..4749)
                     /locus_tag="PKN52_01045"
                     /inference="COORDINATES: protein motif:HMM:NF025793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HNQ98551.1"
                     /translation="MANTLDHRALLQVALEEARQGLTEGGVPIGAALFRTDGTLLGRG
                     HNRRVQDGDPSSHGETDAFRNAGRQRTYKDTIMVTTLEPCWYCSGLVKQFGIPTVVVG
                     ESRTFKGGAAWLREQGVRVIDLDDEECVRLMTEFIAAKPDVWNEDIGEE"
     gene            5019..>5256
                     /locus_tag="PKN52_01050"
     CDS             5019..>5256
                     /locus_tag="PKN52_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98552.1"
                     /translation="MNVRRVVSMLVAAVAVLVGLAAAQGPVNVYAVYATAIEEPWDGV
                     IHAALLDAQKAGLINYEFVDDLGYAGDLERIMREV"
BASE COUNT          718 a         1838 c         1826 g          874 t
ORIGIN      
        1 aggcgggcgt gccgacgatc gtgccgacgc gtgtgagcgc ggtgcgctcc gagggctcgg
       61 ctgacgtgac gatcagcgga gcgagcttcg tgggggcgcc ggcctcaccc gccctcacgc
      121 tgttcgactc cccgcggttc acgctgtccg cgacaactgt tgtgggcttc ctcgacggca
      181 tctacctcga gcgcagtgac tccgtgcgcg tcgtcgggaa ccgcttcgag caggcggctc
      241 gctacgctct gcacgtcatg ttcagcaacg acgtcgtgtt ggaggggaac acggtgcgag
      301 gtggtaacgt cgggtccgcc gccatgtacg gccggggact gcgtgcccgc ggcaacgtct
      361 tcgccggtca tacgggaccc ctggcgttcg gcctcctcat ccaagaactc gccgaggccg
      421 aggtgagcgg caatgcgttc atcgggaaca cggtcggcgt cctggtcgtc tccgcgccgg
      481 acgtcacgat cagcgacaat ctgttggccg acaacggctt cgccatgctc gtgcagcgga
      541 ccaggaccgg cggcaccagc gcagtcaggc tgcaggccaa ctctttcgaa ggcaacgtga
      601 gcgacgttgc gctggacgat ccggaagccg cggtgagcgt cgttggcaac catttcgagg
      661 gcgccagccg gctggacctc gatcacgacg gcgtctccga cgtcccgcac ctgcccagct
      721 cgtcctacga catgtttgcc tcgcgccagc cggacctgtc gctgtttgcc ctcaacccgg
      781 ggatactcct gtggcagacc gccgaggtga cggtgcctgc cctgcggctg gccaccttga
      841 ccgacgccgc cccggcactc cggccgagcg tgggcgcggc agcggcgggc gtcgtcgcgg
      901 gcaaggacac cgcaggcccc gccacgggag cacccgtagg ccgggagccg accggcatcg
      961 ggaccagcac ggtcatggct ctcgggctgg tggccgcctg cgtctttctg ggcgccttcc
     1021 tcggccggcc agtcggtgag tcctcatgag ccccctgctt gcggagcgca cggagcggga
     1081 gcccgcgggc accggcgttc acgcggtaag cgtggcggcg ctcgagcggc gcggtcggct
     1141 cgaccgggtc accttcgacc tcggtgccgg cgcgctcgcg ctcatcggac cgaacggcgc
     1201 gggaaagtcg accatcctgc acgtgctggt agggcggata cggagccacg gcggcgcggc
     1261 cctcctgttt gggcacaagc cgcggaccgt agccgcggcg cggttgcggg cttacgtgcc
     1321 gcagcagata gcgttcccac cgcacctgcg agttagggag gtcctcgcgg ccgcctccaa
     1381 ggcgaaaggc tgcgccaccg agcgggcaga ggccgcggct gcgcggatgg gcatcgacgg
     1441 gttcatgtcg cgcagggcag ggacactgtc aggcggcatg acccaacggc ttgcgctggc
     1501 agccgcgctc atggacgagc cgtccttatg gctattggac gagcccgcct ctgccctgga
     1561 tggcgggggg ctcgaacgct tggctgactg ggctcgggag catgtgcgcg cgggcggaac
     1621 gctcgtcgtg agcgctcatc gccccgaaga ggtcgaggct tttgcctcag aggccatcct
     1681 gttggtacgt ggccgggtcg tcggccgcgt gccagtccac gaactcttcg acgaccggct
     1741 cgtgacgacc ggcgggcggg cgccgctgcc ggaaggcacc tacgtggagc gcagcgccac
     1801 gccgctactg cgcagagtgc tgggcggcga tgagtaggcc ctgccctcaa gccgccggtc
     1861 ggttccggat ccggtggctc ctcgtgctgg ccgcgtgcct ggcgctacca tgctccacgt
     1921 ctatctcctt ggcataccgg ccgaagatgc accacgctga ggactgtccg atgcctcacg
     1981 agaaccaggg ggcgcccacg attctcagcg taccggcgcc cctgccgccg ccgaccgtgg
     2041 taccggccgt ggcggtacca gcgcggttcg aactgccgct ttcgcagccg gtcttcgccg
     2101 ccgtcgcagc gcgcgccggg cgcaaccgcg acccgccacg accgcccgtc gccgaccctg
     2161 aagcctgaat cacaccgggt ccgcgccggt gcgcaacagc gaccgtgcgt acggcgggct
     2221 ggacctcttg agcgttcccg cggccgtggc gccgcgaaag gggatgacgt gacggtgaca
     2281 cacgatcgta ggaccttcct gaagaagctg gcggtggcct ccgtggccgc ccccctgaca
     2341 ctcggtctcg cgcgggccca gatgggccac atggggggag gcgacaccgg tatgggcggt
     2401 atgggcggca tgatgagcag tccttcgctc ggcatcggcc ccgacatcct ggccgcggac
     2461 gagattccgt gggacaccgg catctgcgcc ttctgcggga tgaccatcgc cacacccgcc
     2521 gacggtccta acccggcggg gttccgcgag cgcacgtacg ctcagatccg gctggcgcct
     2581 cagcacgaag gcggcatgcc ctccacgctg cactacgagt ccctggcatg catgttcaac
     2641 cacgcctaca cccttggcat cgccgacggc cacggagcta ccttctacgt cgccgatctc
     2701 tcggcgccgc caaggacggc cgcggacctg gtgttgtcga gggcggcgac gttcctgtgg
     2761 gcgtccgagt tgagcgtttc gatgcaagcg cggctcggcg cgttccgtga ccaggcggca
     2821 ctgcatgctt acgtctccca cgaggaggtc ggacgccatc atgtgctcga cgccgagcta
     2881 cttgccgacc tggccccgat gcccgaggcg ggactgctcg ggctgctcac gaagcactcc
     2941 gagtagccgt ggccaaaggg atcgaccgcc gcacgttcat ccggaagacg ggccacacgc
     3001 tcggcgcggt cctcctctgg tcgcagcgag catgggcgga cccgcggcct gtcaccttcg
     3061 gcgtggacgt gtgcccatac tgcaacatga cggtcgtcga cctgcgcttc accgcgcagg
     3121 tcgtcacccc caccgggctc gtacaccact acgacgccat cgagtgcttg gccgatcatc
     3181 tgatgggtca cggaccgagg ccgcccgagg tcgccgagac gtatctggcg gatcgggcgg
     3241 tcagcagcag ggacgaggcg gcgttccttg aggtcgagga ggcggtcgtg ctacatcacg
     3301 cgcgcctgcg caccccgatg gggggcgggc tggcggcgtt cgctgaccgc gctgccgccg
     3361 aggagttcgc cgccgccggc aggttagccg gggcggagta cttgacctgg cgcgaggtgc
     3421 tggagcgcgg tcgcgaccgc ccgtgggttc ctgactactg agatgggagc tggcatggcc
     3481 ggggttcact tgcgcgaggt cctgcgcaac ccatggacgt tgttcgccgg cctagcgctc
     3541 gtcggcatca gcctggcatt cagcggccgt ggcagcgccg tagggctggt cagcctggtg
     3601 gccgtgcttc agctctactt gccgccctta acggtcgtgg tggccgcgcc cctcctgacg
     3661 aggcgagaga cgtgggcgtt ctgggcagcg ttaccgaggc ccgcgggcgc cgcctaccgc
     3721 ggcgccaccg tcggcatagc cgtgggactg ctgctgcctc tcttggccgg tacaggcgcc
     3781 gccgcggcca tcctcgggct tggtgcgctc gagctgcttc tcctcatcgc cacggtcgcg
     3841 gcgctcgtgg tgctctggac gtgcacggtc tcgttcctca gcgcgcttac cctcgacccc
     3901 acccgggcga tggctctcgg cctcgccgcg tgggcgctgc tcgtgctcgc ctatggaccg
     3961 acggtcgtcg gcctagcggt agccttcgcc gactatccac tcgggccctt catgctcctc
     4021 gccatcgccc tgaaccccac tgaactcctc cgggtagggc ttctccaggc tctcggtgtt
     4081 ccggtgctcg tcggcccggt cgggaagctc gtagtggaca ccgtcccgaa cggcggtttc
     4141 gggctgggcg tcgcggccgt gcttatgctc tccgccctgt tgctctggct gtccggactc
     4201 tggttcgcgc gccgggatcg ctgaggttgg tcgcctgggt cgtggctggg agttcgtacg
     4261 cgcgctcggc cacaccgccc cttcggtaac gcgtcactcc tccccgatgt cctcgttcca
     4321 gacgtccggc ttggcggcga tgaactccgt catgaggcgc acgcactcct cgtcgtcgag
     4381 gtcgatcacc cgcacgccct gctcgcgcag ccacgcggcg ccgcccttga acgtgcgcga
     4441 ctcgccgacg accacggtgg ggatgccgaa ctgcttgacg aggccgctgc agtaccagca
     4501 cggctcgagg gtggtgacca tgatcgtgtc cttgtacgtg cgctgcctgc cggcgttgcg
     4561 gaacgcgtcc gtctcgccgt gcgagctcgg gtcgccgtcc tggacgcggc ggttgtggcc
     4621 gcggccaagc aacgtcccgt ccgtgcggaa gagtgccgcg ccgatgggga cgccgccctc
     4681 ggtcagcccc tgccgcgcct cctcgagggc aacttgcagg agcgcccggt gatcgagcgt
     4741 gttagccatg cctcgcctcc taagctagct gcccaggaag ctagctagct gtccaagacg
     4801 ctaccgcaaa gagcgcgacc tgagcgggac gccgcggtgc tgcgccggcg cgctcggctt
     4861 gggagaacgg ccggcttcgc agccccgccc ttgccgccgc cgcgcttgcc gctatactcg
     4921 ccgaggaacg cgtaagtaga ccccgcggct taggtcgcgg gcgcttgtgc ggatcgcgcg
     4981 ggaggcgcct gcggctagtg caggacgtca gaaagggcgt gaacgtgaga agagtcgtgt
     5041 ctatgctcgt cgccgccgtg gcggtgctgg tcggcttggc cgccgcgcag ggccccgtga
     5101 acgtctacgc cgtttacgcc acggccatcg aggagccatg ggacggcgtc atccacgccg
     5161 ccctgcttga cgcccagaag gccggtctca tcaactacga gttcgtcgac gacctcggct
     5221 acgctggcga cctcgagcgc atcatgcgcg aggtcg
//
LOCUS       DAOQYE010000027         5096 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_16654_length_5096_cov_3.158104, whole genome shotgun sequence.
ACCESSION   DAOQYE010000027 DAOQYE010000000
VERSION     DAOQYE010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 5096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5096
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16654_length_5096_cov_3.158104"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..629
                     /locus_tag="PKN52_01055"
     CDS             <1..629
                     /locus_tag="PKN52_01055"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HNQ98553.1"
                     /translation="RARVSKPVVTLLLAHPSAESLSAALARAARAALTDADTDVRMHD
                     LYADGFDPRMPASEVGTTELADELVARYVADVLATDAFVIVHPVWFFNVPAILKGWID
                     RVMREGIVYEHGPTGMVGLLKARSALLVNTANGGEELERGFGGNALVNLWERVILGSS
                     GVTDFRRLRFGGVARSSNEVRRAWLAEVDDAARSLVADVKSRLDIGHE"
     gene            complement(700..1182)
                     /locus_tag="PKN52_01060"
     CDS             complement(700..1182)
                     /locus_tag="PKN52_01060"
                     /inference="COORDINATES: protein motif:HMM:NF017355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HNQ98554.1"
                     /translation="MSPNPNAKTPPKRGGVGIPKRPATQRPTQPKTAQPASGARTGMM
                     RHQGYTAKVIYDDASGLLRGEVIDLREDIAFSAPAVAQLRREFHAAVDGYLLDCATRG
                     VEPAKPFSGKVLLRMPPDLHRKAAITAAALDVSLNDFLVASIEVGVARIAAVRPDDQS
                     "
     gene            1449..2381
                     /locus_tag="PKN52_01065"
     CDS             1449..2381
                     /locus_tag="PKN52_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta(1)-pyrroline-2-carboxylate reductase
                     family protein"
                     /protein_id="HNQ98555.1"
                     /translation="MLVFDAAETAALLPYPELAAEIAAVLRAAARGEVSALERGTLPL
                     PGGGTFLSMAAADAQAAIVKVGSVHPLNVGRGLPTVQAVVVAVDAVDGRPRVVLDGTT
                     VTTRRTAALSLLAARCVKARVRTVLVVGAGTQARGHVEAFAAGSAVERVLVHGRDAGR
                     AAALAQYVGALGLAAEVIEPGSLRQAVEAADTVITTTNSSTPVLGDVVAPSLRPETTL
                     VAVGAFTPAMAELAPAVVGRCDVIVDTLAGARAEAGDLIQAVAAGAWGWNRAGELVGV
                     LEGFERGPRPVLFKSVGHSMFDLAATRLALRSSS"
     gene            2424..2867
                     /locus_tag="PKN52_01070"
     CDS             2424..2867
                     /locus_tag="PKN52_01070"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HNQ98556.1"
                     /translation="MHERIDYSKASPAVLEAMVALERACKRLGIERSLYELVKIRASQ
                     LNGCAYCLAMHTKDARAAGESDERLDLLAAWREAEGFYTERERAALAWTEALTLLPDA
                     GAPDDVYEAALAQFGEGGLADLTLLIVTINGWNRFGVGFALRPGR"
     gene            complement(2931..4148)
                     /locus_tag="PKN52_01075"
     CDS             complement(2931..4148)
                     /locus_tag="PKN52_01075"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNQ98557.1"
                     /translation="MSSDATADVAVIGAGIVGAAAAFRLAEAGAGVIVLEAADAPATG
                     STGKSAAGVRVQFSEAVNVELSWASIQEYRAFHDLYGASSGYDPLGYLFLVPDEHAAA
                     YRASVDLQRRLGAPVAELEPAEARTLVPFVADGVAMATFGSADGVIDPHAVTLTYLAM
                     AKRHGARVLVGSPVRSAAWDGSAWRLGVAEAAAGAASGPGHVSRTVSAGMVVNAAGAW
                     AGDVAAQAGLRVPVVPMLRSIYATAPLSEPHRYPLTIDVASGYYLRSEGRRVIMGRSN
                     LEQPPGFFEGVDMQDLERVAAFGMARFPWLAAVGLDRRASWWGYYEVTPDDQPIIGRM
                     PPVAPGASDWLNACGFSGHGVQQAAMVGRLIAEEALRGSATSLDIAPLRYERFLGANG
                     EFGAIGRRELNIV"
     gene            complement(4162..>5096)
                     /locus_tag="PKN52_01080"
     CDS             complement(4162..>5096)
                     /locus_tag="PKN52_01080"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNQ98558.1"
                     /translation="TGGVVLPLGVGIAFGLLVGMPLGEAVFVGTILTATSVSISAQTL
                     LELRKLRTKEGMTILGAAVIDDVMGILILSVVVALFAGGGTAVPIWLVAVKMVAYFAV
                     GIALAPVVKALLGWFDRLPIAQPLVALALVLVLFYSWAAEYWGGVAAITGAYLVGILI
                     GQTAFKHRLEKATQVFAYGFFVSIFFVDIGLRANLREALGGPLVWVGLAIIVIAILTK
                     VLGAGLGARAMGFSNFEALRVGAGMVSRGEVGLIVAAIGVERGVIPQDLFAIMVLMVV
                     VTTLVTPMFLRAAFKLGPPSDEPRDEMRPAAEGA"
BASE COUNT          774 a         1749 c         1828 g          745 t
ORIGIN      
        1 cacgagcgcg cgtgagcaag ccggtcgtga cccttctctt agcgcatccg tcagcggaga
       61 gcctgtcagc cgcgctggcg cgcgccgcca gggccgcgct cacggacgcc gacacggacg
      121 tgcgtatgca cgacctgtac gccgacggct tcgacccgcg tatgccggcc agcgaggtcg
      181 gcaccaccga gttggccgac gagctcgtgg cccgttacgt cgccgacgtg ctggccacgg
      241 atgccttcgt gatcgtgcat ccggtctggt tcttcaacgt cccagccatc ctgaaggggt
      301 ggatcgaccg ggtcatgcga gaaggcatcg tgtacgagca cggtccgacc ggcatggtgg
      361 ggctgctcaa ggccaggtcg gcgctgctgg tgaacaccgc caacggcggc gaggagctcg
      421 agcgcggctt cgggggcaac gcgctcgtca acctctggga gcgcgtgatc ctgggctcaa
      481 gcggtgtgac cgacttcagg cgcctgcgct tcgggggcgt ggcccgatcg tcgaacgagg
      541 tgaggcgggc ttggctcgcc gaggtggacg acgcggcgcg ttcgctcgtg gccgacgtga
      601 agagcagact agacatcgga cacgagtagc tccatgccgc ggtccgggga gcgtgagggg
      661 ttgttcgacg acctggtgcg cttaggcgcg cgtcctccgt caggactggt cgtcggggcg
      721 taccgccgcg atccgtgcca ctcccacctc gatgctggcc acgaggaagt cgttcaggct
      781 cacgtcgagc gcggccgccg tgatggctgc cttcctatgc aggtcgggcg gcatacggag
      841 caagaccttt cccgagaacg gcttggccgg ctccacccca cgcgtggcgc agtcgagcag
      901 gtagccgtcg acggcggcgt ggaactcgcg ccgtagctgc gcgaccgccg gggcggagaa
      961 ggcgatgtct tcccggagat cgatgacctc cccgcgcagc aggccgctcg cgtcgtcgta
     1021 gatgaccttc gccgtgtagc cctggtggcg catcatgccg gtcctcgccc ccgacgctgg
     1081 ttgagcggtc ttgggttgcg tgggcctctg cgtagccggt cgcttgggga tgccgacacc
     1141 tccgcgcttg gggggcgtct tggcgttcgg gttcgggctc aaggagggcc tccgctcgcg
     1201 cgatcatcaa tggtatctaa agacagtatc attaaacctt ccgagtgacc ggccgctccg
     1261 gagccgaccc gagtccggtg ggtggtcggg ggtctccgcg ggccataggt ctctacgggc
     1321 atggggaaga atgatatcga atgacggtat tgaatacaag gtcggcgagt tcacggctcc
     1381 aggacctgct cgggcggcat gagcctcgcg ggcttgcagg atggcgcggt ggcgggcgaa
     1441 ccttgggcat gctcgtgttc gatgccgccg agaccgccgc cctactcccg tacccggagc
     1501 tcgccgccga gatagccgcc gtgttgcgcg cggccgcgcg cggcgaggtg agcgctctgg
     1561 agcgcggcac gctgccgctg ccaggcggcg gcacgttcct ctcgatggcc gctgccgacg
     1621 ctcaggccgc catcgtcaag gtcggtagcg tgcacccgct gaacgtcggc agaggcctcc
     1681 cgaccgtcca ggcggtggtc gtcgccgtcg acgcggtcga cggccggccg cgggtcgtcc
     1741 tggatgggac caccgtgacg acgcggcgca ccgcggcgct gtcgctgctg gccgctcgct
     1801 gcgtgaaggc gcgcgtacgg acagtgctgg tcgtcggcgc cggcacgcag gcgcgcgggc
     1861 acgtcgaggc gttcgccgcg gggtcggcgg tggagcgcgt gctcgttcac ggccgggacg
     1921 ccggcagggc cgccgcgctc gcccagtacg tcggcgcttt gggcctcgca gcggaggtca
     1981 tcgagccggg atctctgagg caggccgtcg aggccgccga caccgtgata acgacaacga
     2041 actcctcgac tccggtgctg ggagacgtcg ttgcgccctc gctccgcccc gagacgaccc
     2101 tggtcgccgt cggcgccttc acgcccgcga tggccgaact ggctccggcg gtggtcgggc
     2161 gctgcgatgt catagtcgac accctcgccg gcgcaagggc ggaggcgggc gacctgatcc
     2221 aggcggtcgc ggccggcgcc tggggctgga acagggccgg cgagctcgtc ggagtactgg
     2281 aaggcttcga gcgcgggcct aggccggtac tcttcaagag cgtcgggcac tccatgttcg
     2341 acctggcagc gacgcggctg gcattgaggt cgagctcttg agttccgacg ccgccctcga
     2401 cgggggcggg atgggaggac ctgatgcacg agaggatcga ttactccaag gcctcgccgg
     2461 cagtgctgga ggccatggtc gcgctggaga gggcatgtaa acgcctgggt atcgagcgct
     2521 cgctctatga gctggtcaag atccgggcgt cgcagctcaa cggttgcgcg tactgcctgg
     2581 ccatgcatac gaaggacgcg cgggcggcgg gcgagagcga cgagcgcctc gacctcctgg
     2641 ctgcctggcg cgaggcggaa ggcttctaca cggagcggga acgagccgcc ctcgcctgga
     2701 cggaggctct cactctgctc cccgacgccg gggctcccga cgacgtgtac gaggcagccc
     2761 ttgcccagtt cggagaaggc gggctcgccg acctgacgtt gctcatcgtc accatcaacg
     2821 gttggaaccg cttcggcgta gggttcgcct tgcgtccggg tcgctgacgg cccggcggcc
     2881 cactggcgcg acgccgccat tgcgacgccg cggtccgtca tgcacgcggt tcagacgatg
     2941 ttcaactctc gccgtccgat cgcgccgaac tcgccgttcg cgcccaggaa gcgctcgtag
     3001 cgcagggggg cgatgtcgag gctcgtagca ctgccccgca gggcctcctc ggcgatcaac
     3061 ctgccgacca tcgccgcctg ctgcacgccg tgacccgaga agccgcaggc gttcaaccag
     3121 tcggaggcgc ccggcgccac cgggggcatg cgcccgatga tgggttggtc gtccggtgtc
     3181 acctcgtaat agccccacca ggacgcgcgc cggtcaaggc cgacagcggc cagccatggg
     3241 aagcgggcca tgccgaacgc ggcgacgcgc tccaagtcct gcatgtcgac gccctcgaag
     3301 aaaccgggcg gttgctccag gttcgaacgc cccatgatga cgcgtcgccc ctcgctcctc
     3361 aggtagtagc cactcgcgac gtcgatggtg agcgggtagc ggtgcggttc cgacaggggg
     3421 gccgtagcgt agatgctgcg cagcatgggc acgaccggca cccgcagccc ggcctgcgcc
     3481 gccacgtcgc cggcccacgc gccagcggcg ttgacgacca tgcccgcgct gacggttctg
     3541 ctcacgtgcc ccgggccgct cgcggcgccc gccgcagcct ccgcgacgcc cagccgccag
     3601 gccgagccgt cccacgcggc cgagcgcacg ggcgagccta cgaggacgcg cgcgccgtgc
     3661 cgcttagcca tggcgaggta cgtgagcgtg accgcgtggg ggtcgatcac cccgtctgcg
     3721 cttccgaacg tggccatggc gaccccgtcg gcgacgaagg gcacgagcgt ccgggcctcg
     3781 gccggctcca gctccgccac cggcgcgccg agccgccgct gaaggtcgac cgatgcgcgg
     3841 taggcggccg catgctcgtc gggcaccaag aagaggtagc cgagcgggtc gtagcccgag
     3901 ctggcgccgt agaggtcatg gaaggcccgg tactcctgga tcgaggccca cgagagttcg
     3961 acgttgacgg cctcgctgaa ctggacccgc accccggccg cgctcttgcc cgtcgagccg
     4021 gtggcaggcg catcggcggc ctcgaggacg atcacgccag ccccggcctc cgccagcctg
     4081 aacgcggcgg ccgcgccgac gatgcctgcg ccgatgaccg ccacgtcggc ggtggcgtcg
     4141 gaactcatgg accggcctcc ctcaggcgcc ctcggccgcc gggcgcatct cgtcgcgcgg
     4201 ctcgtccgag ggcggtccga gcttgaacgc cgcccggagg aacatcgggg tgacgagcgt
     4261 agtcaccacg accatcaaca ccatgatggc gaacaggtcc tgcgggatga ccccgcgctc
     4321 gacgccgatg gccgctacga tgaggcccac ttcgcccctc gacaccatcc cggcgccgac
     4381 gcgcagcgcc tcgaagttgc tgaagcccat ggcgcgcgcc cccaagcccg ccccgagcac
     4441 cttcgtcagg atggcgatga cgatgatggc caagccaacc catacgagtg ggcctccgag
     4501 cgcctcgcgc aggttggcgc gcagaccgat gtccacgaag aagatggaca cgaagaagcc
     4561 gtacgcgaag acctgtgtcg ccttctcgag ccggtgcttg aacgccgtct ggccgatgag
     4621 gatgcctacg aggtaggcgc ccgtgatggc ggccacgccg ccccagtact ccgccgccca
     4681 ggagtagaag aggacgagca ccagcgcaag cgccacgagc ggttgggcga tgggcagcct
     4741 gtcgaaccac ccgagcagcg ctttgacgac cggggcgagc gcgatgccga cggcgaagta
     4801 ggccaccatc ttcacggcca cgagccagat gggcacggcg gtgccgccgc ccgcgaagag
     4861 cgccacgacg acggagagga tgaggatgcc catcacgtcg tctatcacgg ccgcgccgag
     4921 gatggtcatg ccctccttcg tgcgcagctt cctcagctcg aggagcgtct gggcgctgat
     4981 ggagacgctc gtcgcggtca ggatggtgcc gacgaacacg gcctcgccga gcggcatgcc
     5041 gactaggagg ccgaaggcga tgccgacacc gagcggcagc acgacgccgc cggtcg
//
LOCUS       DAOQYE010000028         5078 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_16745_length_5078_cov_3.337050, whole genome shotgun sequence.
ACCESSION   DAOQYE010000028 DAOQYE010000000
VERSION     DAOQYE010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 5078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5078
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16745_length_5078_cov_3.337050"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..141)
                     /locus_tag="PKN52_01085"
     CDS             complement(<1..141)
                     /locus_tag="PKN52_01085"
                     /inference="COORDINATES: protein motif:HMM:NF015691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamB/YcsF family protein"
                     /protein_id="HNQ98559.1"
                     /translation="MTIDLNADLGEGFGVWLGPGDDASLLRHVSSANVACGFHAGDPT
                     IMR"
     gene            complement(227..2182)
                     /locus_tag="PKN52_01090"
     CDS             complement(227..2182)
                     /locus_tag="PKN52_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching N-terminal
                     domain-containing protein"
                     /protein_id="HNQ98560.1"
                     /translation="MTVTEIAPNQFGRNLVLKENYTFLVANEAAAVQGGEHGLYSRDT
                     RYLSRYAWSVGWDESTDAREAQLLLDDSPRPDQCRQHVAVMAGPTQVLAIARRLDIAA
                     AALRDSLSITNTSLTRQSVVLTLAFEADFADLFQARGWHERPVKFEGGTVSGAPGGAS
                     VSFDHVASDGLGQGLTLTFSPAPNEVSVSAATYALDLRPGESVELAVEALIRDPLETD
                     PVAADPLEAGQRGAAPPGISYKNWLGSFRPLLTSDGPSAGRHGAVLRRAALDLRALML
                     FSEHGPVAAAGVPWFVTAFGRDSLLTALMLLDRRPDVARGTLRYLAALQGRVHDPERS
                     EAPGKILHEVRQGELARTGKVPFGRYYGSIDATLLFMVLLHETYRRDGDLGLLRELRP
                     NLEAALRWLASDADPDGDGFVEFAGAEGGKGLSVQSWKDSNDALSHADGTLARGAVAV
                     AEVQGYAYAALLAAAECLTALGEDEAAERRVARAKELKERFHEAFWLPSLRTYALALD
                     HDKRPLEVLASDAGQLLWTGIVPKAFAPTLAATLMSDALFSGWGIRTLGTGEVRYNPL
                     SYHNGSVWPHDTALIAAGLRRYGFTQHASTLRDALFDLAASQPDLRPPELVAGYERSD
                     SPPVPYPAACRPQAWSSAALVYLSDWA"
     gene            complement(2179..3576)
                     /locus_tag="PKN52_01095"
     CDS             complement(2179..3576)
                     /locus_tag="PKN52_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNQ98561.1"
                     /translation="MSVSSAAAGAAILARERWEARRRWSRAAIAYLVMLIFSILFLGP
                     LLFAALSSVKTDPLAYPPKLLGPQLSPANWAAAASLGRQGAGAPLWGGFAPGSTVTFE
                     LTYFAPDGLEAQAPSVSVPRRRPGGGLGAVQRITYAADYAEVGDVTEVGREPADGGQL
                     VTYRVQVTYPGEGPTIDRLPLDAQSPSREQRFVGSTLPPTRLERLGLVASYDSIAPGA
                     LGYLLGNYRRVFTEARSVTTGQSLFLRWTSNTFVYAFFRVLITLAIATTAGYALARLR
                     FPGRNLVFLLVLFAQMVPTQVLFVSNYLVLRDGIWGLASLWGQSTLLNGLPGLLFVTA
                     LNAGPVFIMKQFFESLPREVEESAMIDGASYWSRFSRVVLPMARPAIGALVILSFQGA
                     WNDFFWPLVILTTPEDVKTLPIGLLTFRSVYGNSGDWGLILAGAILSALPVIVLFVVF
                     QRYFMEGVSYGGGKE"
     gene            complement(3573..4712)
                     /locus_tag="PKN52_01100"
     CDS             complement(3573..4712)
                     /locus_tag="PKN52_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNQ98562.1"
                     /translation="MPRSGRELLPALLFLSPFLVVLAIFFVYASARAVYFSFTDYDLF
                     NAPKWVGLENYRALFKDDNFLYALRNSLVFAAVVTAVQTAGALVTASALNRRVRGMTF
                     FRSAFYMPAVTSSVVITLIFLWLFQRLGGINYLITAVARNAPAIGLFVLVAIVVQAIQ
                     VVLERRRWRGATGGASKRTVAAFDPALLVVSLLVAAAATIGLGLLGYLAPRGTDSVAI
                     NWLQTRQEVPNGAPFFLRAPLPLVAIMVQNTFTTIPTFMLMFLAALQDVPRSYYEAAA
                     LDGANPAQQFFYITVPSLQPVIFLVVSLGLIGTLQMFDQVAIFGSAVPLQSVITLAYF
                     VYNRMFPGAQLPEVGFASAAAMFLAALTLVIVLLQRTVLRAEGDR"
BASE COUNT          831 a         1668 c         1891 g          688 t
ORIGIN      
        1 acgcatgatc gtcgggtcgc ccgcgtggaa gccgcaggcc acgttggccg aggagacgtg
       61 gcggagcagg ctcgcgtcgt cgcccggacc gagccagacg ccgaagcctt cgccgaggtc
      121 ggcgttgagg tcgatcgtca tggggtcatg ctaccccccg gcctcggccg gagtagccgg
      181 ccgcgccgcg ccgccgggta cagccaacgg caacgaccgt cgaggctcag gcccagtcgc
      241 tcaggtagac gagcgcggcc gacgaccagg cctgcggcct gcacgccgcc gggtagggca
      301 cgggcggcga gtcggagcgc tcgtagccgg ccaccagctc aggcggccgc aggtcgggct
      361 ggctggccgc gaggtcgaag agcgcgtcgc gcagcgttga ggcgtgctgg gtgaacccgt
      421 agcggcgcag gcccgcggcg atcagcgcgg tgtcgtgcgg ccacacggag ccgttgtgat
      481 agctcagcgg gttgtaacgc acctcgcccg tgccgagtgt tcgtatgccc cagccggaga
      541 agagcgcgtc cgacatgagg gtggccgcga gcgtcggcgc gaacgctttc gggacgatcc
      601 cggtccatag gagctggccg gcgtcggacg ccaggacctc gagcgggcgc ttgtcgtggt
      661 cgagggccag ggcgtaggta cggaggctcg gcagccagaa cgcctcgtgg aaacgctcct
      721 tcagctcctt cgcacgcgcc acgcggcgct ccgcagcttc gtcctcccca agggcggtca
      781 ggcactcggc ggcggccagc agcgcggcgt aggcgtagcc ttggacctcg gccacggcga
      841 ccgcgccgcg cgccagcgtg ccgtcggcat ggctcagggc gtcgttggag tccttccacg
      901 actgcacgct caggcccttg ccgccttcgg cgcccgcgaa ctctacgaag ccgtcgccgt
      961 cggggtccgc gtcggaggcc agccagcgca gggcggcctc gaggttgggc cggagctcgc
     1021 ggagcaggcc aaggtcgccg tcgcgcctgt acgtctcgtg cagcaacacc atgaagagga
     1081 gcgtcgcgtc gatgctcccg tagtagcgcc cgaacggcac ctttcccgtg cgagcgagct
     1141 ccccctgcct gacctcgtgc aggatcttgc cgggcgcctc ggagcgctcg gggtcgtgca
     1201 cgcggccctg gagggcggcg aggtagcgca gggtgccgcg cgccacatcc gggcgccggt
     1261 cgaggagcat cagcgccgtg aggagggagt cgcgaccgaa ggcggtcacg aaccacggca
     1321 cgccggccgc cgccacgggg ccatgctcgg agaagagcat gagggcccta aggtcgagcg
     1381 cggcgcggcg caagacggcg ccatgccgac cggcggaagg cccgtcgctc gtcagcagcg
     1441 ggcggaagga ccctagccag ttcttgtagg agatccccgg gggcgccgcc ccgcgctggc
     1501 ccgcctcgag cgggtccgcc gccaccgggt ccgtttcaag cgggtcccgg atgagcgctt
     1561 ccacggccag ctcgacgctc tcgcctggcc gaaggtcgag cgcgtacgtg gcagccgaga
     1621 cgctcacctc gttgggcgcc ggcgagaagg tcagcgtcag cccctgcccg agcccgtcgc
     1681 tcgccacgtg gtcgaagctc acggatgcgc cgcccggcgc cccactcact gttccgccct
     1741 cgaacttgac cggacgctcg tgccaaccgc gcgcctggaa gaggtcggcg aagtcggcct
     1801 cgaaagcgag cgtcagcacc acggactgcc gcgtcagcga ggtgttggtg atcgagaggg
     1861 agtcgcgcag ggccgccgcg gcgatgtcga gccgccgcgc gatggccaag acctgcgtcg
     1921 gcccggccat cacggccacg tgctggcggc actggtccgg ccgcggggag tcgtcgagga
     1981 ggagctgggc ttcgcgggcg tcggtggact cgtcccaccc gacactccac gcgtagcgcg
     2041 acaggtagcg cgtgtcgcgg ctgtagagcc cgtgctcgcc gccttgcacc gccgctgcct
     2101 cgttggccac gaggaacgtg tagttctcct tcagcacgag gttgcggccg aactggttgg
     2161 gcgcgatctc cgtgacggtc actccttgcc tcctccgtac gagacgccct ccatgaagta
     2221 gcgctggaac acgacgaaca agactatgac aggcagcgcg ctcaggatgg ccccggccag
     2281 gatgagcccc cagtcgcccg agttgccgta gacgctgcgg aacgtgagca ggccgatggg
     2341 gagcgtcttg acgtcctccg gcgtggtgag gatgacgagg ggccagaaga agtcgttcca
     2401 tgccccctgg aacgagagga tgacgagcgc tccgatggcc gggcgcgcca tcggcagcac
     2461 gacccgcgag aaacgcgacc agtagctggc gccgtcgatc atggccgact cctcgacctc
     2521 gcgcgggagc gattcgaaga actgcttcat gatgaagacc ggccccgcgt tgagcgcggt
     2581 gacgaagagg aggcccggca ggccgttgag gagagtcgac tggccccaga gcgacgccaa
     2641 cccccagatg ccgtcgcgca gcaccagata gttgctgacg aacaggacct gggttggcac
     2701 catctgcgcg aacagcacga ggaggaacac gaggttccgt ccagggaagc gcaggcgtgc
     2761 gagcgcgtag cccgccgttg tggcgatggc cagggtgatc agtacccgga agaaggcgta
     2821 cacgaacgtg ttggacgtcc agcgcaggaa gaggctctgg cccgtcgtga cgctgcgcgc
     2881 ctcggtgaac acgcggcggt agttgccgag caggtagccg agcgctccgg gcgcgatcga
     2941 gtcgtacgaa gccacgaggc cgaggcgttc cagccgcgtg ggcgggagcg tcgagccgac
     3001 gaaacgctgc tcgcgggagg gcgactgggc gtcgaggggc aggcggtcga tggtcggtcc
     3061 ctctcccggg taggtgacct gcacgcggta ggtgacgagt tggccgccgt cggccggctc
     3121 cctccccacc tcggtaacgt cgccgacctc ggcgtaatcg gccgcgtacg tgatcctctg
     3181 cacggcaccc agcccgccgc ccggccgcct gcgcggcacg ctcacgctcg gagcttgcgc
     3241 ctccagcccg tcgggcgcga agtaggtgag ctcgaaggtc actgtagaac cgggtgcgaa
     3301 gccgccccac agtggcgcgc cggcgccctg tctgccgagg ctcgccgccg ccgcccagtt
     3361 ggcgggggag agttgcgggc ctagcagctt cggcgggtag gcgagagggt ccgtcttgac
     3421 gctcgacagc gcggcgaaga gtaggggccc gaggaacagg atggagaaga tgagcatcac
     3481 caggtaggcg atcgcggcgc gtgaccaccg cctgcgcgcc tcccagcgct cgcgggccag
     3541 gatggcggcg cccgccgcgg cggacgagac gctcatctgt cgccctccgc gcgcagcacc
     3601 gtgcgctgga gcagcacgat gacgagggtg agcgcggcga ggaacatcgc cgcagccgac
     3661 gcgaagccga cctccggcag ctgggcgccc gggaacatgc ggttgtagac gaagtaggcg
     3721 agggtgatga cgctctgcaa cggcacggcg ctaccgaaga tggcgacctg atcgaacatc
     3781 tgcaacgtgc ctatgagccc gagcgacacc acgaggaaga tgaccggctg cagcgacggg
     3841 acggtgatgt agaagaactg ctgcgccggg ttggccccgt cgagcgccgc cgcctcgtag
     3901 tacgagcgag gcacgtcctg gagggcggcc aggaacatga gcatgaacgt cgggatggtc
     3961 gtgaaagtgt tctgcaccat gatggccacg agcgggagcg gcgccctgag gaagaacggc
     4021 gccccgttcg gtacctcctg gcgcgtctgc agccagttga tggcgaccga gtcggtccct
     4081 ctcggcgcca ggtagccaag gaggccgagg ccgatggtcg cggcggcggc cacgagcagg
     4141 gagactacga gcagggcagg gtcgaacgcg gcgaccgtgc gcttggaggc tccccctgtc
     4201 gcgccgcgcc agcggcggcg ctcgagcacc acttggatgg cctggacgac gatggcgacg
     4261 aggacgaaca gcccgatggc gggggcgttc ctggcgaccg ccgtgatgag gtagttgatg
     4321 cccccgaggc gctggaagag ccagaggaag atcagcgtga tgacgacgct gctcgtgacc
     4381 gcgggcatgt agaaggcgga gcggaagaac gtcatgccgc gcacgcggcg gttgagtgcg
     4441 ctggccgtca cgagcgctcc agccgtctgc accgcggtca cgacggccgc gaagacgagc
     4501 gagttccgca gcgcgtacag gaagttgtcg tccttgaaga gcgcgcggta gttctcgagc
     4561 cccacccact tcggggcgtt gaagagatcg tagtcggtga aagagaagta gacggcacgg
     4621 gcgctggcgt agacgaagaa gatggccaac accacgagga acggagatag gaacaggagc
     4681 gcaggcagta gctcgcgacc gcttctcggc atggcgcttg cctccatgag tggcttgggt
     4741 tccgggaccc gagaacctgt ggccggttgc ccgggtggcg cgctagtgct gggaggcgcc
     4801 cgctggacgc ctccctaacc gaccgtatcg cttggtgccc tgccccggcg ctgcttgggt
     4861 cgagcgccgg gacagggcac ctgccggcgc cttagggcgc gccttaaggc gcggcgcgct
     4921 gcaggatggc ggtgagttcg ccctgggcct ggctgagcgc ggcctggctg ccggaggcgc
     4981 cggtcatgcc caccgtcagg gcgttgttga tgggccccat gtagtcggta ccgacggtgc
     5041 cgaactggaa cccgtagacg tcgccgtgcg aggcgccc
//
LOCUS       DAOQYE010000029         5060 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_16817_length_5060_cov_2.798801, whole genome shotgun sequence.
ACCESSION   DAOQYE010000029 DAOQYE010000000
VERSION     DAOQYE010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 5060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5060
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16817_length_5060_cov_2.798801"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1447
                     /locus_tag="PKN52_01105"
     CDS             <1..1447
                     /locus_tag="PKN52_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HNQ98563.1"
                     /translation="DENEMLAFRDRFNLPITDDQIANEPFHLPAEDSPEHQYLVEHRE
                     KLGGFLPARSVQSEPLAPPPAEAFEEFMASSGDHTVSTTMVIVNIMRKLLRDPNWSKL
                     VVPIIPDEARTFGMEAFFRQIGIYSPVGQLYEPVDRAHLMYYKESRDGQILEEGITEA
                     GGMASFIAAGTAYANYGVNTVPFYLYYSMFGYQRVGDLIWLAGDSHARGFLVGATAGR
                     TTLAGEGLQHQDGHSHVLTYPVPNLLSYDPSFGHELAIIVRDGLRRMYQEQEKVFYYV
                     TVGNENYLQPGAPSHLSREELEEGVLKGMYLFRPASRKRSKLHAQLFGSGSIMNCVLE
                     AQEILERDYGVATNVWSVTSYKELHRDALQVERYNRLNPEQPQHKPYVTQKLEGAKGV
                     FVAASDYMKILPDALAAYLPRPMLSLGTDGYGRSEARSELRDFFEVDARHVVYATLTA
                     LHRDGLVDTAALVAARDALEIEPAKADPYVD"
     gene            1470..2909
                     /locus_tag="PKN52_01110"
     CDS             1470..2909
                     /locus_tag="PKN52_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HNQ98564.1"
                     /translation="MPTEVKLPDIGEGIEKGTVVGVLVKVGDTVAKDQPLVELETDKA
                     VVEVPSSASGVVSKINVTENQEARVGSVLVVVDEEASEPAAAAGGVSAAASAAASAPA
                     SEPAPTAPQDAAPSASVAPASTPSAAAVPAQAAPQASEPRQQAAPGAQPQPGPALGAA
                     ANEAGGGTVPAAPSVRRLARELGVDIRAVPGSGVLGRISAADVRAYADGVASGSAVAG
                     PRAAVPAGSAPAAAQVAAPLPDFSRWGETERTPMSGVRKATVRSMTNAWSSVPMVTQF
                     DKADITELEVVRKRYQPKAEALGAKLTPTAILLKVVAGALRRFPDFNASIDLATQEIV
                     HKRYVNVGVAVDTDAGLLVPVVKNADRKNMLELAVELGELAVKARNRKLTPDEMQGGN
                     FSISNLGGIGGYAFTPIVNPPDVAILGVSRSSMEPVWNPDAGQFEPRLMLPLSLTYDH
                     RLIDGAAAARFLRWVCTALEDPFIIALEG"
     gene            3127..3900
                     /gene="lptB"
                     /locus_tag="PKN52_01115"
     CDS             3127..3900
                     /gene="lptB"
                     /locus_tag="PKN52_01115"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HNQ98565.1"
                     /translation="MRAVDSVSNGVSPVPNGIELRASGLVKRYGRRKVVDGVDLVLRR
                     GEIVALLGPNGAGKTTSFYMVVGFVRPNSGRITLGEQEITAWPMHERARIGLGYLAQE
                     PSAFRRLSVIDNLLAILEFQDLGRAEQEERAKALLEEFHIAHLAKSRADTLSGGERRR
                     LEIARSLTVDPDFLLLDEPFTGVDPKSIREIQTIVRDLRDRRGLGVLLTDHSVRETLA
                     IADRVILMFDGRVRFDGTPADFAADPDVRTYYLGNEFQL"
     gene            3976..>5060
                     /locus_tag="PKN52_01120"
     CDS             3976..>5060
                     /locus_tag="PKN52_01120"
                     /inference="COORDINATES: protein motif:HMM:NF022727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3084 domain-containing protein"
                     /protein_id="HNQ98566.1"
                     /translation="MTLVALLVLLLVVLAGVIAYLGDRLGTYVGRRRLSLFGTRPRTT
                     GQLVGVLSGILIMLTTIGVLALAFQNATRTILNTQRTLDELNQLRSQQRVLNQEVADA
                     NRQLADVRSQLEEAQRTIATAEAQRDAAQEARDTAQQERDALQTQRDELASQVADAEQ
                     RVADADAEVNAAEERLHDTQAELDDATTALADARADRDRALVEADEARSAAEQAQEET
                     ARLGQGLADARSQLADANAELLRLQGDLVDVELRLATAEQQVQSAQDDLSAAREARDA
                     ALADRDAAFVDREAALADRDAANLDRDAALDQAADLSDRLVDLNAEVGALEASTEELR
                     KQAQSLRDENTNLATTNELLSSENARI"
BASE COUNT          846 a         1851 c         1668 g          695 t
ORIGIN      
        1 cgacgagaac gagatgctcg cgttccgcga ccgcttcaac ctgcccatca ccgacgacca
       61 gatcgccaac gagccgttcc acctcccggc ggaggacagc cccgagcacc agtacctcgt
      121 cgagcatcgc gagaagctag gcggcttcct gcccgccagg agcgtgcagt ccgagccgct
      181 ggcccccccg cccgccgaag ccttcgagga gttcatggcc tcgagcggcg atcacaccgt
      241 ctcgacgacc atggtgatcg tgaacatcat gcgcaagctc ctgcgcgacc cgaactggag
      301 caagctcgtc gtgcccatca tcccagacga ggcacgcacc ttcggcatgg aagcgttctt
      361 ccgccagatc ggcatctact cgcccgtcgg gcagctctac gagcccgtgg acagagcgca
      421 cctcatgtac tacaaggagt cgcgcgacgg ccagatcctc gaagaaggca tcaccgaggc
      481 gggcggcatg gcctccttca tcgcggccgg caccgcctac gccaactacg gcgtcaacac
      541 ggtgcccttc tacctctact actccatgtt cggctaccag cgcgtcggcg acctcatctg
      601 gctggccggt gacagccacg ccaggggctt cctggtcggc gcgacggccg ggcgcacgac
      661 actggccggc gagggcctcc agcatcagga cggccactcc cacgtcctca cctacccggt
      721 ccccaacctc ctctcgtacg acccgtcttt cggccacgaa ctggcgatca tcgtccgcga
      781 cggcctgcgc cgcatgtacc aggagcagga gaaggtcttc tactacgtca cggtgggcaa
      841 cgagaactac ctccagcccg gcgcgccctc gcacctgtcg cgcgaggaac tggaagaggg
      901 cgtgctcaag ggcatgtacc tgtttcgtcc ggcgagccgc aagcgcagca agctgcacgc
      961 gcagctcttc ggcagcggca gcatcatgaa ctgcgtgctc gaggcgcagg agatcctcga
     1021 gcgcgactac ggcgtcgcca ccaacgtctg gagcgtcacc tcctacaagg aactccaccg
     1081 cgacgccctc caggtcgagc gctacaatcg cctcaacccg gagcagccgc agcacaagcc
     1141 gtacgtgacg cagaagctcg aaggcgcgaa gggcgtgttc gtggccgcct cggactacat
     1201 gaagatcctc ccggacgcgc tcgcggccta cctgccccgc cccatgctct cactcggcac
     1261 ggacgggtac ggccggagcg aggcgcgcag cgagctccgc gacttcttcg aggtcgacgc
     1321 ccgccacgtc gtatacgcca cgctgaccgc cctgcaccgc gacggactcg tcgacacggc
     1381 ggcgctcgtc gcggcccgcg acgccctcga gatcgagccg gccaaggccg acccgtacgt
     1441 cgattgatgc ccgccgaaag gaactgctcg tgccaactga agtgaagcta cctgacatcg
     1501 gcgaggggat cgagaagggc accgtcgtag gagtcctggt caaggtcggc gacacggtcg
     1561 ccaaggacca gccgctcgtc gagctggaga ccgacaaggc cgtcgtcgag gtgccgtcgt
     1621 ccgccagcgg cgtcgtgagc aagatcaacg tcaccgagaa ccaggaggcc agggtcggct
     1681 ccgtcctcgt cgtcgtcgac gaggaggcct ccgagccggc cgccgccgct ggcggggtct
     1741 ccgcggccgc ctccgcggcc gcctccgcgc ccgcctccga gccggcgccg accgcgccgc
     1801 aggatgccgc cccgtcggct tccgtggctc cggcttcgac cccgtcggct gctgcggtgc
     1861 cggcccaagc ggcaccccag gcctccgaac ctcggcagca ggccgctccg ggcgcgcagc
     1921 cgcagccggg acccgcgcta ggcgcggcgg cgaacgaggc cggcggcggc accgtgccgg
     1981 ccgcgccgtc cgtgcggcgg ctcgcacgcg aactgggcgt ggacatccgc gcggtgcccg
     2041 gcagcggcgt cctcggccgc atctcggcag ccgacgtgcg cgcttacgcc gacggtgtcg
     2101 cgagtggctc ggccgtggcc ggtcctcggg ccgcggtccc cgcaggctcg gcgccggcgg
     2161 cggcccaggt cgcggccccg ttgcccgact tctcgcgctg gggcgagacc gagcgcactc
     2221 ccatgagcgg cgtgcgcaag gccaccgtgc gctctatgac caacgcctgg tcgagcgtgc
     2281 cgatggtgac ccagttcgac aaggccgaca tcacggagct ggaagtcgtg cgtaaacgct
     2341 accagccgaa ggccgaggcg ctcggcgcca agctcacgcc taccgccatc ctcctcaagg
     2401 tcgtggctgg cgcgctccgg cgcttccccg acttcaacgc cagcatcgac ctcgcgacgc
     2461 aggagatcgt ccacaagcgc tacgtgaacg tcggcgtggc tgtcgatacg gacgccggcc
     2521 tcctcgtccc cgtggtgaag aacgccgatc gcaagaacat gttggagctg gcagtagagc
     2581 tcggtgagct ggcggtcaag gcccggaacc gcaagctgac gcccgacgag atgcagggcg
     2641 gcaacttctc catctcgaac ctgggcggta tcggcgggta cgccttcacg ccgatcgtca
     2701 acccacccga cgtggccatc ctcggcgtct cgcgctcgag catggagccg gtctggaacc
     2761 ccgacgccgg gcagttcgaa ccgcggctca tgctgccact gtcgctcact tacgaccacc
     2821 gcctcatcga cggggccgcg gccgcgaggt tcctgcgctg ggtgtgcacc gcgctcgagg
     2881 acccgttcat catcgccctt gagggctgaa ccgggttctc ccggacttcc ctctgactag
     2941 ggagcgggcc gctgtcaacg cggcccgctc cccacgcggc ccgctcccca cgccgcccgg
     3001 gcgatcggtc tcccgctagc ggcgccggcg gaccggcccc gcccgtggcc ggccaagcga
     3061 gatgggtcgc gcgctacgaa gagcttcacc tggctctcac cggtccgcgt gataccatgc
     3121 cccggtatgc gggccgtcga tagcgtctcc aacggcgtct cgccggtgcc gaacgggata
     3181 gagctgcgcg cgagcggcct ggtgaagcgg tacggcaggc gcaaggtcgt tgacggggtc
     3241 gacctggtgc tccgcagggg cgagatcgtc gcactcctag gccccaacgg ggcgggtaag
     3301 accacgagct tctacatggt cgtcggcttc gtccgtccca actccggtcg cataacgctc
     3361 ggtgaacaag agatcacggc ctggccgatg cacgagcgcg ccaggatcgg tctcggttac
     3421 ctcgcgcagg agccgagcgc cttcaggcgc ctcagcgtca tcgacaacct cctcgccatc
     3481 ctcgagttcc aggacctcgg ccgcgccgag caggaggagc gcgccaaggc gctcctcgag
     3541 gagtttcaca tcgcccacct cgccaagagc cgggccgaca ccctgtcggg cggcgagcgc
     3601 aggcgactcg agatcgccag gagcctcacc gttgaccccg acttcctgct gctggacgaa
     3661 ccgttcaccg gcgtggaccc caagtccatc agggagatcc agacgatcgt ccgcgacctg
     3721 cgcgataggc gcggtctagg cgtcctgctc acggaccaca gcgtgaggga gactctcgcc
     3781 atcgccgacc gcgtcatcct gatgttcgac ggtcgcgtgc gcttcgacgg cacgcccgcc
     3841 gacttcgccg ccgacccgga cgtccgtacg tactatctcg gtaatgagtt ccagctctga
     3901 tcgcggtccc tggggcgctg agccccgtcg ccgcggggtc gggatgacga ggccgcgcgc
     3961 gcaggagccg gagtcgtgac cctggtagcc ctgctcgttc tcctgctcgt cgtgctggca
     4021 ggtgtcatcg cgtacctggg tgaccgcctt gggacatacg tcggccggcg acgcctcagc
     4081 ctcttcggca ccaggcctcg caccaccggc caactcgtcg gcgtgttgtc aggcatcctg
     4141 atcatgctca cgaccatcgg cgtgcttgcg ctcgcgttcc agaacgccac gcgcacgatc
     4201 ctcaacacgc agcgcacctt ggacgagctc aaccagttga ggtcccagca gcgcgtgctc
     4261 aaccaggaag tggccgacgc gaaccgccag ctcgccgacg tgagaagtca gctggaggag
     4321 gcgcagcgga cgatcgcgac cgccgaggcg cagcgcgacg ccgctcagga ggcccgcgac
     4381 acggcccagc aggagcggga cgccctccag acgcagcgcg acgagctggc gagccaggtg
     4441 gccgacgcag agcagcgcgt ggcggacgcc gacgcggaag tcaacgcggc cgaggagcgg
     4501 ctgcacgaca cgcaggccga gctggacgac gccacgacgg cgctcgccga cgccagggcg
     4561 gatcgggaca gggcgctggt cgaggccgac gaggcgcgct ccgccgcgga gcaggcgcaa
     4621 gaggagacgg ccaggctggg gcagggcctc gcagacgcga ggtcgcagct cgccgacgcc
     4681 aacgccgaac ttctgcggct gcaaggcgac ctcgtcgatg tcgagctgcg ccttgcgacc
     4741 gcagaacagc aggtccagag cgcccaggac gacctgagcg cggcgcgcga ggcgcgcgat
     4801 gcggcgctgg cggatcgcga tgccgcgttc gtagaccgcg aggccgcact ggccgaccgc
     4861 gacgcggcga atctggatcg cgacgccgcg ctagaccagg ccgccgacct ctccgatcga
     4921 ctcgtggacc tcaacgccga agtcggggcg ttggaggcca gcacggagga gttgcgcaag
     4981 caagcgcagt cgctgcggga cgagaacacc aacctcgcta ccaccaacga gctgttgtcg
     5041 agcgagaacg cccgcattca
//
LOCUS       DAOQYE010000030         5024 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_16955_length_5024_cov_3.290803, whole genome shotgun sequence.
ACCESSION   DAOQYE010000030 DAOQYE010000000
VERSION     DAOQYE010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 5024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5024
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16955_length_5024_cov_3.290803"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(243..899)
                     /locus_tag="PKN52_01125"
     CDS             complement(243..899)
                     /locus_tag="PKN52_01125"
                     /inference="COORDINATES: protein motif:HMM:NF028169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNQ98567.1"
                     /translation="MPETRRTTRTTRREQAEARRLQIIDAALNLFAQHGYAGTSTKQI
                     AQEVGITEGLIFHYFPTKAELLIAVARQRMTFLAEALELLAGLEDLPAREVLGSIVLG
                     WVEVIQSQADIVTMLLTESQTNAELGGAFRGVVDQLAGALSAYLTSRVRAGELRADLP
                     VRASALMFFSSLMMFFLTNRGLPPEEWRARASRFTGEMLDTWFRGALATTAGRTAEPA
                     "
     gene            1237..2352
                     /locus_tag="PKN52_01130"
     CDS             1237..2352
                     /locus_tag="PKN52_01130"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HNQ98568.1"
                     /translation="MTVQEAKTWLLAAAAKRGVELEVLSTSERQLMLEARGGKPSEVK
                     SSTRAGIGLRVVDDGRTGYASTEELTTDALAWALDEAVANAAITPAAGGALPRGRALG
                     RTDLLGEGLSAPLTDKIATSVSFEQAVTADPRVQAMQFGRYMEAEQQVEIGSTAGVDG
                     GYRAGIALLLGGIVMREGESVKQGYHLDPAGDYHQLDPGRTAQTMLRRVGRQLGAKPL
                     KTGRRRAVFEPEVVTTLLELLVYSLSGKSVVEGKSRLAGKLGERIASPLVTLVDDATL
                     PGALGSRPFDSEGTASARLTLVEEGVLRSFMHNSVTARRTGQPNTGHASRTYSSTLSV
                     SPTNLLLLPGAGIARPTACWSPTSWAFTPAPTPSAAR"
     gene            2292..2549
                     /locus_tag="PKN52_01135"
     CDS             2292..2549
                     /locus_tag="PKN52_01135"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HNQ98569.1"
                     /translation="MLVTDLMGVHAGANPISGEVSVQAMGIETVGGEPRPVDDFAISF
                     DLFELFERVSAVGDDPTWSTDGSTIVNAPSLCVEDVSFAGG"
     gene            complement(2694..3953)
                     /locus_tag="PKN52_01140"
     CDS             complement(2694..3953)
                     /locus_tag="PKN52_01140"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNQ98570.1"
                     /translation="MRPSRSFLYQCLASSFVTAASSMVAVMTPLYAVSLGVEAQWLGL
                     LMALPGIIPVLLALQVGRWVDSIGVARSFFLGIAGLLLSPLTVIVFPGIAALAAAGVL
                     LGLFVLVFTLASQSLVASLTNGKSHESNFGVQSTWLAGGRMVGPILVGTIIDGAGYRI
                     SFAAVFAMVLVAAVLAYEVARAAPRASGGRLRPSPLRKGAVSSTLRNVGLQMAVLTSA
                     GVFLALTMREAFLPVMLEGMGMSATVIGSLVSLGSLTSVLIRPAMPAVIRLLGGAGNA
                     LVTSMVAVAVGVGLLSLAQSVAGFAALAVTVGLGTGIAFPLSIVSVASHVPLRDRGVA
                     LSLRLSFNSTVEIFGPMLSGVVVAATSFRFGFAAAGVTLGCITLVSLAIVKRFNAGAI
                     LGEGAGTERAGPQPEPERGGAVGAAED"
     gene            4223..>5024
                     /locus_tag="PKN52_01145"
     CDS             4223..>5024
                     /locus_tag="PKN52_01145"
                     /inference="COORDINATES: protein motif:HMM:NF019533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HNQ98571.1"
                     /translation="MSEPGRSLPDGDPATTAWLDGLMAWREQRAASLVGPHGWWSITC
                     LEWLEEGENTIGSAEGSAVRLAERFPTEVARVNVAGERATLVPAAGASLLLGGEPFTG
                     QFTADGDVAFVADDLPPPSPHAASAATSTAGRRPARVSVVRRGDLWGVRVHDPVAAER
                     RSVERDLAWFDPAPEWVIEAEFLPPEAEEEVPVSNVIGQVSMHRVAGRVRFVHAGTSW
                     TLLATEAGEGRLFINFRDASNEDLGAAEEAADGRAAGEGVAAEDPRGHH"
BASE COUNT          829 a         1767 c         1774 g          654 t
ORIGIN      
        1 ccaccgtgac cgccaagaag acgccgatga cgacccggct gtgctgtagc gcgaagcggg
       61 cgacgtgggt catgtggggc cacgtccggc ggaacgggcg cggagcgcgc gctcggggcg
      121 ggcgccggtg cggagctggt ggtgaggtcc cggagtcatg ttcagcgctt ctccattctg
      181 atgaccaagt acttgctcaa ttaacaggag acggtcgttc gccgctgcgg tgcccccctc
      241 gctcaggcag gctcggcggt tcgaccggcg gtggtcgcga gggccccgcg gaaccaggta
      301 tccagcatct cgcccgtgaa gcgcgaagcg cgcgctcgcc actcctctgg tggcaggccg
      361 cggttcgtca ggaagaacat catgagagac gagaagaaca tcaacgcact cgcccgcacc
      421 ggaaggtcgg cgcgcagctc gcccgcccgg acgcgcgacg tgaggtaggc cgacagcgcc
      481 cccgcgagct ggtcgacgac cccgcggaac gcccctccca gctccgcgtt cgtctgcgac
      541 tcggtgagga gcatggtgac gatgtccgcc tggctctgga tgacctcgac ccagccgagc
      601 acgatgctgc cgagcacctc gcgcgccggc aggtcctcga gacccgcaag gagctcgagc
      661 gcctcggcga ggaacgtcat gcgctgccgc gccacggcga tgagcaactc cgccttggtg
      721 gggaagtagt ggaagatgag gccttccgtg atcccgacct cctgcgcgat ctgcttcgtc
      781 gacgtaccag cgtagccatg ctgcgcgaag aggttgagcg cggcgtcgat gatctgcagg
      841 cgccgcgcct cagcctgctc gcggcgggtg gtcctcgtgg tccttctcgt ctccggcacc
      901 taaccccttc tttcgttaag tatccactca gtcacttctg ttagaagctt acgggccgac
      961 gcagcatccg tcaaggccac gtacgcgttc tggtcctgtg gctcgtgggc gggtcggaag
     1021 gtggggttcc ggctcgccgg gagcccacac gtgaggatca tgccttcccc ctccggtcgt
     1081 ttgccgccgg ccagggcatc ttcgggtctc ggtccccgcc gcccgccccg ccaagctggt
     1141 accgtcatgg cgatccgctg tcgggaggcg gccgagcaca ctcctctctc gggaccgagc
     1201 gggacccgag ccccgacgac gtaagaaggg tggcgcatga cagtccaaga agccaagacc
     1261 tggctgctag ccgcggccgc aaagcgcggc gtcgagctcg aggtcctctc caccagtgag
     1321 cggcagctca tgctcgaggc gcgcggcggc aagccgtccg aggtcaagtc gtccacgcgg
     1381 gccggcatcg gtctcagggt ggtcgacgac ggccgcaccg gttacgcctc gacggaggag
     1441 ctgacgacgg acgccctcgc gtgggcgctg gacgaggcgg tggcgaacgc cgccatcacg
     1501 ccggcggccg gcggcgccct gccgagaggc cgggccctcg gacgcacgga cctgctcggg
     1561 gagggcctct cggcccccct caccgataag atcgccacgt ccgttagctt cgagcaagcc
     1621 gtcaccgcgg acccccgggt gcaagccatg cagttcgggc gctacatgga ggcggaacaa
     1681 caggtcgaga tcggctccac cgccggggtc gacggcggct accgcgccgg catcgcgctc
     1741 ctgctcggcg gcatcgtcat gcgcgaaggc gagagcgtga agcagggcta ccatctcgac
     1801 ccggccggcg attaccacca gctcgacccc ggccgcaccg cccagaccat gctcaggcgc
     1861 gtcgggaggc agctcggcgc caagccgttg aagaccggca ggcgccgcgc cgtgttcgag
     1921 ccggaagtgg tgactaccct cctggagcta ctcgtctact cgctctccgg caagtccgtg
     1981 gtcgaaggca agagccgtct ggcgggcaag ctcggcgagc gcatcgcctc gccgctcgtc
     2041 accctcgtcg acgacgccac gctgcccggg gccctcggca gccgcccctt cgactcggaa
     2101 ggcacggcga gcgctcgcct cacgctcgtc gaagaaggcg tgttgcgctc gttcatgcac
     2161 aactccgtca ccgctcggcg caccggccaa cccaacacgg gtcatgccag ccgcacctac
     2221 tcgagcaccc tgagcgtcag cccgacgaac ctgttgctcc tgccgggcgc cggcatcgcc
     2281 aggccgacgg cgtgctggtc accgacctca tgggcgttca cgccggcgcc aaccccatca
     2341 gcggcgaggt gagcgtgcag gccatgggca tcgagacggt gggcggcgag ccccgcccgg
     2401 tggacgactt cgccatctcg ttcgacctgt tcgaactctt cgagcgcgtg agcgcggtcg
     2461 gcgacgaccc gacgtggtcg accgacggca gcaccatcgt caacgcgccg agcttgtgcg
     2521 tggaggacgt cagcttcgcg ggcggctaac gccccgccag aagctgaatg ctccgcggga
     2581 ggctaacgcc ccgccagaaa ccgaatgctc cgcgggaagc tgacgccccg tcggaagccg
     2641 agtacttcgc aggcggctaa cgccccgcca gaaaccgaag atctcgcggc cggtcaatcc
     2701 tcggccgctc ccaccgcccc accccgctcg ggctcgggct gcggcccggc ccgctcggtg
     2761 ccggcgccct cgcctaggat ggcgccggcg ttgaagcgct tcacgatcgc cagagacacg
     2821 agcgttatgc accccagcgt cacaccggcc gcggcgaagc cgaagcggaa ggacgtcgcg
     2881 gccacgacga ccccgctcag catcggaccg aagatctcca ccgtgctgtt gaacgagagg
     2941 cgcaggctga gggcgacgcc gcggtcgcgc agcggcacgt gcgacgccac cgacacgatg
     3001 ctcagaggga aggcgatgcc cgtgccgagg ccgaccgtga cggccagggc cgcgaagccg
     3061 gccacgctct gcgccaacga gagtaggccg acgccaacgg ccacggcgac catggaggtg
     3121 acgagcgcgt tgccggcgcc gccgagcagc cggatgaccg cgggcatggc cggcctgatc
     3181 aacaccgagg tgagcgagcc gaggctgacg agcgagccga tgaccgtggc ggacatgccc
     3241 atcccctcga gcatcacggg gaggaacgcc tcccgcatcg tcagggcgag gaagacgccg
     3301 gcgctcgtga gcacggccat ctgcaacccg acgttgcgca gcgtcgagct gaccgcgccc
     3361 ttgcgcagcg ggctcgggcg gaggcgccca ccggacgctc gcggcgctgc cctagccacc
     3421 tcataggcga ggaccgccgc taccagcacc atggcgaaga cggccgcgaa gctgatgcgg
     3481 taaccggcgc cgtcgatgat ggtgccgacc aggatggggc cgaccatccg cccgccggcc
     3541 agccaggtgg actggacgcc gaagttgctc tcgtgcgact tgccgttggt gagcgatgcc
     3601 acgagcgact gcgacgcgag cgtgaagacg agcacgaaca ggcccagcag caccccggcg
     3661 gccgcgagcg cggcgatccc ggggaacacg atcaccgtca ggggcgagag gagcaggccc
     3721 gcgatgccga ggaagaacga cctggccacg ccgatggagt cgacccaccg tccgacctgc
     3781 agcgccagca ggaccgggat gatccccggc aacgccatga ggagcccgag ccactgcgcc
     3841 tcgacgccca ggctgacggc gtagaggggc gtcatgacgg ccaccatgct ggaggccgcc
     3901 gtcacgaagc tcgaggcgag acattggtag aggaacgaac gagatggtcg catcgtgcgg
     3961 taatgaagtc gcgtgccggt actgctcgag tgtcacgcgc ggccccaccg ccggacttcc
     4021 ggcatagggt aaccccgaac ggacggcgat gggaatgagc gggcgccgga ccggtagcca
     4081 cgccgctagt ccggccctcg ccccacggcc ctaggcgcga gacgcgctcg tctgccgcct
     4141 cgaagcagga ggcggccttc acctacatgg tcgtaggcgc gagacgcgcg ccaccccggc
     4201 gcgagcctag aatcgacggg tgatgagcga acctggacgg tctctccccg acggcgaccc
     4261 cgcgacgaca gcctggctgg acggtctcat ggcctggcgc gagcagcgtg ctgcgagcct
     4321 cgtcgggcct cacggctggt ggtcgatcac gtgcctggag tggttggagg agggcgagaa
     4381 caccatcggc agcgcggagg ggagcgcggt caggctggcc gagcgcttcc cgaccgaggt
     4441 ggctcgcgtg aacgtcgccg gcgagcgcgc gacgctcgta ccggcggcgg gggcctccct
     4501 gctcctcggc ggggagccgt tcaccgggca gttcacggcg gacggcgacg tggccttcgt
     4561 cgccgacgac ctaccgccgc cgagcccaca cgcggcgagc gccgccacgt cgaccgccgg
     4621 tcggcgaccc gcgcgcgtca gtgtcgtgag gcgcggcgac ctctggggcg tccgggtgca
     4681 cgatcccgtc gcggccgaac ggaggagcgt cgagcgcgac ctcgcctggt ttgacccggc
     4741 gccggaatgg gtgatcgagg cggagttcct cccgcccgag gcggaggagg aggtgccggt
     4801 cagcaacgtc atcggccagg tgagcatgca ccgcgtggcg gggcgggtcc gcttcgtaca
     4861 tgcaggcacg agctggacgc tgctggcgac ggaagccgga gaggggcgcc tcttcatcaa
     4921 cttccgcgac gcgagcaacg aggacctggg cgcggccgag gaagcggcgg acggacgcgc
     4981 agccggcgag ggcgttgcgg ccgaagaccc gcgcggccac catg
//
LOCUS       DAOQYE010000031         4996 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_17091_length_4996_cov_3.118397, whole genome shotgun sequence.
ACCESSION   DAOQYE010000031 DAOQYE010000000
VERSION     DAOQYE010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 4996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4996
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17091_length_4996_cov_3.118397"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            220..1134
                     /locus_tag="PKN52_01150"
     CDS             220..1134
                     /locus_tag="PKN52_01150"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNQ98572.1"
                     /translation="MIVETRLMDGGRVNQVVEKEAKVVNGFVGFALTLVAILGGGALI
                     VLGAYQEAWYLVVLGILLALGGVFAANGLVAVQPNQARVLTFFGSYAGSVRRSGFWYV
                     NPFTVKKNVSLRVRNFTSERIKVNDADGNPVEIAAVVVWRVAESAKAVFDVDDYEQFV
                     AIQAETALRGLATRYPYDSAGEDRPSLRENPEELANALRLELEERLQVAGVDVIEARL
                     THLAYAPEIAQAMLRRQQAQAVVSARRLIVEAAVGMVEDALTGLSERGVVELDSDKRA
                     AMANNLMVALTSEQSVSPVVNAGTLYGG"
     gene            1146..2954
                     /locus_tag="PKN52_01155"
     CDS             1146..2954
                     /locus_tag="PKN52_01155"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNQ98573.1"
                     /translation="MSRGSTLTPHRPALRTKFLAGLAGLLASFAPIATAQDTVGAGEP
                     APVAYDLSVGGAVVGEFGVALTRAVEGATGGEARSHLQVPGAFDLADHLVTAENGAAR
                     SYVLAGTFQGVAISIEVAFASDAAAFTIEQAGQKQQLSLPLPGDVYVMDNNFLDGMQF
                     IVDRAVASGGGLEVDVIVPQAVVLGRMTVAAPVAATVRYGGADIEARRVDATMSVGPQ
                     TATLVAYLDEAGDILVLTSDPGSARFERRPGGAQDAATAAAAEQPTGAATLAAQQEAL
                     AALLSEQAACLVERDVDIVSTGAELVGKLTLPLSAAHGGRPAPTLVLLPGSGPVDLDG
                     NTPPVLLNSGYKQLAYALACRGYGVLRASKLGIAPSTGDGNAVTIATYAQNLADWFAF
                     LTDQPGVDAERLGVMGHSEGGLVALYAVAEGYVSPAAVVLLASPGRPLDVVLKEQILA
                     RNAEAGATQEQLAGLAVQVDELVAAVRASTGVGLELTPELQANPLVPLFANAAGLLRS
                     EFAEDPAALAARVSAPVAVLQGEKDIQIAVGDGEAIAAAAQRADLFLFPDLTHNLVET
                     RGPALGMPLPGPGAQISKTLVQVIATYLAGHLRPGR"
     gene            2970..3251
                     /locus_tag="PKN52_01160"
     CDS             2970..3251
                     /locus_tag="PKN52_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98574.1"
                     /translation="MADKHRFLLRLDQKVHAALEKWAADDLRSLNAQIEYLLADALKR
                     AGRYKDTLGDASGSPNPTPTAQEGPTATAAPKPAPEEGAPQDGAPQEGA"
     gene            complement(3294..4301)
                     /gene="mltG"
                     /locus_tag="PKN52_01165"
     CDS             complement(3294..4301)
                     /gene="mltG"
                     /locus_tag="PKN52_01165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HNQ98575.1"
                     /translation="MVVIVLVALGLSAVRFVLSPVSATTATMEFEVLPGWGGARIADA
                     LEQAGLVRSALAFELLLRFTDLDRKMGEGLYDLAPAMSSREIAATLAAGGRPRTVRIV
                     IPEGWRAEAIVARLAANGLGDELELAALVRSPGELAPPGLPEGATLEGYLFPASYDLP
                     VRTSASQALAALVSRFEDELSGEVVAKLADSGLRVHDWVTLASMVQAEAASAEEMPII
                     AGVFRNRLDDGMPLQSDPTVAYGLGKALPELDALAGDLRKDTPWNTYTQPSLPAGPIG
                     NPGHDALLAVLEPQRLDPDGVPYLYFLHGTDGGVPVFRPNTSLQAHNRDVDRYLRNSG
                     R"
     gene            complement(4382..4906)
                     /gene="ruvX"
                     /locus_tag="PKN52_01170"
     CDS             complement(4382..4906)
                     /gene="ruvX"
                     /locus_tag="PKN52_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HNQ98576.1"
                     /translation="MAEAAGQPPADQPSAPDRPASRQGDRLPTGAKLLALDVGDARIG
                     VAAGTVGSLLAFGRGAIGRQGTRKDVPAVLALVEKEGAAAIVVGIPVRLDGSESPQTE
                     RVRRFAAALAEAGADVVFEDERLTTRIAQRPVSDSDLPRSRRQEKGLLDEAAAVQILE
                     SYLRRLGGPVGGPE"
BASE COUNT          741 a         1803 c         1752 g          700 t
ORIGIN      
        1 accggccctc cactgtccgg ccccccccac ccctgcctac ccatcagaag cagcacacac
       61 catagtgcga acacgccgta gaaacagcac ggcggatgcc gccgctcccg tcgaccagcg
      121 tatagctgac cgccgaagcg accgctcgat gaggcccgta ggctagtgcc atcggtcgcg
      181 cgccggcctg atccggctcg ctatcaatat gatatcatca tgatagtaga gacgagactc
      241 atggacggag gcagagtgaa ccaggtagta gagaaggaag ccaaggtcgt caacggattc
      301 gtagggttcg ccctcacgct ggtcgccatc ctgggcggtg gcgccctgat cgtgctcggc
      361 gcgtaccagg aggcgtggta cctggttgtg ctcgggatcc tgctcgcgct aggtggggtc
      421 ttcgcggcga acggcctcgt ggccgtccag cccaaccagg cgcgcgtcct cacgttcttc
      481 ggctcgtacg cgggctccgt ccgccggtcc ggcttctggt acgtgaaccc cttcaccgtc
      541 aagaagaacg tgagcctgcg cgtgcgcaac ttcaccagcg agcgcatcaa ggtgaacgac
      601 gccgacggca acccggtcga gatcgccgca gtcgtcgtgt ggcgggtggc ggagtcggcc
      661 aaggcggtct tcgacgtcga cgactatgag cagttcgtgg ccatccaggc cgagacggcg
      721 ctgcgcggcc tcgccacgcg ctacccgtac gactcggccg gcgaggaccg cccgtctctg
      781 agggagaacc cggaggaact ggccaacgcg ctgcggctgg aactcgagga gcgcctgcag
      841 gtcgcgggcg tcgacgtcat cgaggcgcgc ctcacccacc tcgcgtacgc cccggagatc
      901 gcgcaggcca tgctgcgcag gcagcaggcg caggccgtcg tctccgccag gcgcctgatc
      961 gtcgaggcgg ccgtcggcat ggtcgaggac gcgctcaccg gtctctcgga gcgcggcgtc
     1021 gtcgaactcg attccgacaa gcgcgcggcc atggccaaca acctcatggt cgcgcttacg
     1081 agcgagcagt ccgtctcgcc cgtcgtgaac gccggcacgc tgtacggcgg ttaggagcag
     1141 tggagatgag ccgcggatcg acgttgacgc cccaccggcc ggcgctgcgc accaagttcc
     1201 tggcaggcct cgctggcctc ttggcctcct tcgcccccat cgcgacggct caagacaccg
     1261 ttggcgctgg tgagcccgcc cccgtcgcct acgacctgtc cgtcggcggc gccgtggtcg
     1321 gggagttcgg cgtcgcgctc acgagggccg tggagggagc cacgggcggc gaggcgcgct
     1381 cgcacctcca ggtgccgggc gccttcgacc tcgcggacca cctcgtcacc gccgagaacg
     1441 gcgccgcgcg cagctacgtg ctggcaggca cgttccaggg cgtggcgatc tcgatcgagg
     1501 tggccttcgc gtccgacgcc gccgccttca cgatcgagca ggccggccag aagcagcagc
     1561 tgagcctgcc cttacccggc gacgtctacg tcatggataa caacttccta gacggtatgc
     1621 agttcatcgt cgaccgggcc gtggccagcg gcggaggcct agaggtcgac gtcatcgtgc
     1681 ctcaggcggt cgtcctgggt cgcatgaccg tcgcggcgcc cgtggcggcg accgtcaggt
     1741 acgggggcgc cgacatcgaa gcccggcgtg tcgacgccac catgagcgtc ggcccgcaga
     1801 cggccaccct cgtcgcctac ctggacgagg ccggcgacat cctggtgctg acgtccgacc
     1861 ccggctctgc gcgcttcgag cggcgaccgg gaggcgcgca ggacgcggcc actgccgcgg
     1921 ccgccgagca gccgaccggc gccgcgacgc tcgccgcgca gcaggaggcg ctcgccgccc
     1981 tcctctcgga acaggccgcg tgcctcgtcg agcgtgacgt agacatcgtc agcacggggg
     2041 ccgagctggt aggcaagctc acgctgccgc tgtccgccgc ccacggaggc cgaccggcgc
     2101 cgacgctcgt cctgctcccc ggttcgggcc ccgtcgacct cgacggcaac accccgccgg
     2161 tgctcctcaa cagcggctac aagcaactcg cctacgcgct cgcctgccgc ggctacgggg
     2221 tgctgcgcgc ttccaagctc gggatcgcgc cgtcgaccgg cgacgggaac gccgtcacca
     2281 tcgctaccta cgcgcagaac ctggccgact ggttcgcctt cctgaccgac cagccggggg
     2341 tcgacgcgga gcgcctcggc gtcatggggc actcggaggg cgggctcgtc gccctgtacg
     2401 cggtagcaga gggttacgtc tcgccggcgg ccgtggtgct gctcgccagc cccggcaggc
     2461 ccctcgacgt ggtcttgaag gagcagatcc tggctcgcaa cgccgaagcc ggggcgaccc
     2521 aagagcagtt ggccgggctg gcggtccagg ttgacgagct cgtagcggct gtccgcgcgt
     2581 cgaccggcgt cggcctcgag ctcactccgg agctgcaggc caacccgctc gtgccgctct
     2641 tcgccaacgc cgccgggctg ctgcgcagcg agttcgccga ggacccggcc gcgctggcgg
     2701 cgcgtgtgag cgcgcccgtc gcggtgttgc aaggggagaa ggacatccag atcgccgtgg
     2761 gagatggaga ggccatcgcg gcagccgcgc agcgggccga cctcttcctg ttccctgacc
     2821 tgacgcacaa cctcgtcgag accaggggac ccgcgctggg catgccgctc ccgggtccag
     2881 gcgcgcagat ctcgaagacg ctcgtccagg tcatcgccac gtacctcgcc ggccacctgc
     2941 ggcctggcag gtgaggtcgg cttagcgcga tggccgacaa gcaccgcttc ctcctgcggc
     3001 tcgaccagaa ggtgcacgcc gccctggaga agtgggcggc cgacgacctg cgcagcctca
     3061 acgcgcagat cgaatacctg ctggccgatg ccctgaagcg ggccggacgg tacaaggaca
     3121 cgctggggga cgcctctgga agcccgaacc ctacgccgac cgcccaggag gggcctacag
     3181 cgaccgcggc ccccaagccg gcgccggagg agggtgcgcc gcaggacggt gcgccgcagg
     3241 agggcgcgta gcaggacgac gcgtagcagg acgacgcctt ccggagggac cccttagcgc
     3301 ccggagttac gcaagtagcg gtctacgtcg cggttgtggg cctgcaggct cgtgttgggg
     3361 cggaagacag gcacgccgcc gtccgtcccg tgcaggaagt agaggtacgg cacgccgtcg
     3421 gggtccaggc gctgcggctc gagcacggcc agcagggcgt cgtgcccagg gttgccgatc
     3481 gggcccgccg gaaggctcgg ctgggtgtac gtgttccagg gggtgtcctt gcgcaggtca
     3541 cctgcgagcg cgtccagctc cggtagggcc ttcccgagcc cgtacgccac ggtagggtct
     3601 gactgcagcg gcatgccgtc gtccagccgg ttgcggaaga ccccggcgat gatgggcatc
     3661 tcctcggccg acgccgcctc ggcctggacc atgctggcca gcgtcaccca gtcgtggacc
     3721 cgcagaccgc tgtcggcgag cttcgcgacg acctcaccag acaactcgtc ctcgaaacgg
     3781 ctcaccaggg cggcgagggc ctgcgacgcg ctcgtgcgca ccggcaggtc ataactggcg
     3841 gggaagaggt agccctcgag cgtggcgccc tccggaagcc cagggggggc caactcgccc
     3901 ggcgagcgga ccaacgcggc caactccagt tcgtctccga gcccgttggc ggccagccgg
     3961 gccacgatgg cttccgcgcg ccagccttcg gggatcacga tgcgtaccgt gcgcggccgc
     4021 ccgcccgccg ccagtgtcgc ggcgatctcg cggctgctca tggccggggc caggtcgtag
     4081 agcccctcgc ccatcttgcg gtcgaggtcg gtgaagcgca ggagtagttc gaacgccagc
     4141 gcggaacgca cgaggccggc ctgctcgagg gcgtcggcta tccgggcgcc gccccagccg
     4201 ggcaggacct cgaactccat ggtggcggtc gtggcgctca cgggcgagag gacgaagcgc
     4261 acggcgctca gcccgagcgc cacgagcact atcacgacca acaacccaag catcaggcca
     4321 cggccgcggc gtttccgcgg tcgcgcggac tccggggggg ttccggcggc tccggcgtcg
     4381 ctcactctgg tcctccgacg gggccgccaa ggcgccttag gtacgactcg aggatctgga
     4441 cggcggcggc ctcgtccagg agccccttct cctgcctgcg cgaccgcggc aggtcggagt
     4501 cgctgaccgg tctctgcgcg atgcgcgtgg ttaggcgctc gtcctcgaag accacgtctg
     4561 ctccggcctc ggccagggcg gcggcgaacc gccgcacgcg ctccgtctgg ggcgactcgc
     4621 tgccgtcgag cctcacgggg atgccgacca cgatggccgc cgccccctcc ttctcgacca
     4681 acgccaacac ggctgggacg tccttgcgtg ttccctggcg gcctatcgcg ccgcggccga
     4741 aggcgaggag cgagccgaca gtgccggcgg cgacgccgat gcgcgcgtcg ccgacgtcga
     4801 gggcgagcag cttggcgccg gtcggcagcc gatcgccctg gcgactggcg gggcggtcgg
     4861 gcgcggacgg ctggtccgcg ggtggctgtc ccgcggcctc agccacgcag ccaactctcc
     4921 acgacctgcg ccaacccgtc ctccgagttg ggcggcccga gcacgtcggc cacttccaac
     4981 gcctcctgct cggcgt
//
LOCUS       DAOQYE010000032         4928 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_17413_length_4928_cov_3.537656, whole genome shotgun sequence.
ACCESSION   DAOQYE010000032 DAOQYE010000000
VERSION     DAOQYE010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 4928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4928
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17413_length_4928_cov_3.537656"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /locus_tag="PKN52_01175"
     CDS             complement(<1..472)
                     /locus_tag="PKN52_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HNQ98577.1"
                     /translation="MSPASVSAARLPVSTRDLLHGKTPLDVLDLEGRHGNGDLLKVLD
                     ILGIAGPFKVLSPWELEDETGRNLIHAGGYAAMPFGEGYPPLVDFVRDYLTENRQLGL
                     PQQSLSEWRAALETNLVALLASVAPSHADSHVYFGNSGAEAVEAALKMARAARPK"
     gene            complement(498..1115)
                     /locus_tag="PKN52_01180"
     CDS             complement(498..1115)
                     /locus_tag="PKN52_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98578.1"
                     /translation="MRATFPIRLGARLVLAGAALLGLGACVHRTDSMAPTITITEPRS
                     GTTRSSDDLRISGYALDDAGIASIRVDGADLMRDSLYASERGKRLVQFAFTKPNLQDG
                     ELTLVLEVTDVNGRSSLERYVLTLDATPPTVELTKVESVSGGRIRVEGVARDNMRLSS
                     IRINGVPLAFTPSTEYSFSVIVDAVSDGQIVVEDSAGNTTTRDLR"
     gene            1423..3165
                     /locus_tag="PKN52_01185"
     CDS             1423..3165
                     /locus_tag="PKN52_01185"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNQ98579.1"
                     /translation="MKRVFTLLATGLLMLGAAFAQAPADTYVYMTFGQPVSMDPARAY
                     DTASGGIIENVYETLMSYDGEALDVFVPSLATGYSVSADGLHWTFDLRPNVKFHSGNL
                     MTCKDVAWSFKYGALTANPEGAPAYLMGNLWLGTDIDGSDPAAFLDAVTWDMIDGIVT
                     CPEGPQGLKATVNLVNQTPALIPILTYTAFSIVDSQFAIDGGAWDGTEATWKDWVGRD
                     LTAEFLHRNASGTGAFKLVDWTDDSVVADAFADYWGGAPALNHVIYRYVDEQSTRILA
                     VQQGDADRITLNENSAVAQVEGAAGVSVLRDPNWLPASVTAAFFNFDIDVTNNEDVGS
                     GLLDGKGIPSNFFADVNVRRGFAQLFDQQAFVDQVYQGQGTVITMGLPSSFLGYNPDV
                     AVRTLDLEAATDYFRAAFDGQVWEKGFEFTALYNSGNTIRQTALELIKDNLEFINPKF
                     KMNVRGLPWADYLSRTAEKKAPMFALGWAADYADPSNFINTFYDDDGYYAARTSISVP
                     EIQAIIDQADQLVDPEARGFLYRGIGTLHYDQAPLIAVPIQSAFLVVRSNLNGVYYNT
                     MYSDKFLWKDLSKN"
     gene            3335..4363
                     /locus_tag="PKN52_01190"
     CDS             3335..4363
                     /locus_tag="PKN52_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ98580.1"
                     /translation="MLVFVARRLLLLPVVFVGVTLAIVLLMQLLTPYQRAAAYVQSEQ
                     QIRNIDSIIVQYGLTDPWYMQYGRWIGQVMKGNLGYSRTSREPVLTTLGKRFPVTLEL
                     AILAILPVIGVGVAMGTAAALNRDKPIDQFARVASIIGWSLPTFVLGIWLLVIFYGWL
                     GWFQPGRISDAIRISTGPNSGFVNYTRMMLVDALLNKRLDVFWDALLHMVLPVVTLSV
                     VQSAQIMRVMRSSLLDALGRDYVRTARAKGLPEHTVTHKHARRNALIPVITLSGFVLI
                     GLINGVVITETIFNYPGLGQWAAASAVNLDYASVLGFAVFTAMMVVLANLLVDILYGV
                     VDPRIRYE"
     gene            4365..>4928
                     /locus_tag="PKN52_01195"
     CDS             4365..>4928
                     /locus_tag="PKN52_01195"
                     /inference="COORDINATES: protein motif:HMM:NF024313.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ98581.1"
                     /translation="MAADLPVAQNPDGALDAHPDQPARKGRAWKRFRRNPLAVVGLIM
                     VAAFVLIAVFAPVITELPRSCLRDLELTAANQHDYRNPTKPAFWKAVFAPPKSCFTIA
                     RVSFSTQPADAATTKTVLGTTSGGYDIFYGLVWGTRTAFKVGLIVVGIAFAIGVIIGS
                     LSGYLGGVFDNLVMRFTDIIISLPSLVV"
BASE COUNT          807 a         1746 c         1488 g          887 t
ORIGIN      
        1 ccttgggtct ggccgcgcgc gccatcttca gcgccgcctc gacagcctcg gcgccgctgt
       61 tgccgaagta cacgtggctg tcggcgtggc tcggcgccac actcgcgagg agcgccacga
      121 ggttcgtctc gagcgctgcc cgccactcgc tcagcgactg ctgcgggaga ccgagctgcc
      181 tgttctccgt caggtagtcg cgcacgaagt cgacgagggg cggatacccc tccccgaacg
      241 gcatcgcggc gtagccgccg gcatggatca ggttgcgacc ggtctcgtcc tccagctccc
      301 aagggctcaa gaccttgaaa gggcccgcga tccccaggat gtcgaggact ttgagcaggt
      361 cgccgttgcc gtggcggccc tcgaggtcga gtacgtctag aggggttttg ccgtgcagca
      421 ggtccctcgt ggagaccggc aggcgggcgg cgctgacgct ggccgggctc atctcgttgg
      481 gctcgccatc tgtgtgctca tctcaagtcc ctcgtcgtgg tgttgccggc gctgtcctcg
      541 acgacgatct ggccgtcgct gacggcgtcg acgatgacgg agaaggagta ttcggtgctc
      601 ggcgtgaacg ccagcggcac gccgttgatc ctgatgctgc tcagccgcat gttgtcgcgc
      661 gccacgccct cgacccggat gcgcccgccc gacacggatt ccaccttcgt cagctcgacc
      721 gtcggcgggg tggcgtcgag cgtcagcaca tagcgctcca gtgacgaacg accgttgacg
      781 tccgtgactt cgaggacgag cgtcagctcg ccgtcctgca ggttgggctt ggtgaacgcg
      841 aactgcacga ggcgcttgcc gcgttccgac gcgtagaggc tgtcgcgcat caggtcggcg
      901 ccgtccacac ggatgctggc tatcccggcg tcgtcgagcg cgtaaccgct gatgcgcagg
      961 tcgtcggaac tcctggtcgt gccgctacgc ggctccgtga tggtgatcgt gggggccatc
     1021 gagtccgtgc ggtgaacgca cgcaccgagg ccgagcaggg cggcacccgc gaggaccagc
     1081 ctcgcgccga gccggatggg gaaggtggcg cgcacggggc aatgctacac cgcgccgttc
     1141 gtcgggcccg cagcaggacc ggcgaagata cgagcgccgt gccagcctcc ggcctcgccg
     1201 cgcaggcgcc gcttggtgat ccgtggtgca agagcgacgg ccgttctgat agagttaccg
     1261 tacgcgtcga aggagggccc tagccctctg tcataggaaa gggggtcccg ccagttcgga
     1321 ccacgaactc gtttccgtga acgttctgcc tgggccaggc gcgtatccga acgtgccccc
     1381 tgccaccacc gaaagacgca acgcgttaag gagtgatagt tgatgaagag agtcttcacc
     1441 ctgctagcca cgggcctgct gatgttgggc gcggccttcg cgcaggcgcc ggccgacacg
     1501 tacgtttata tgacgttcgg ccagcctgtc tcgatggacc ctgcccgtgc ttacgacacc
     1561 gcctccggcg gcatcatcga gaacgtctac gagaccctca tgtcgtacga cggagaggcg
     1621 ctcgacgtct tcgtcccgag cctcgccacc ggctactcgg tcagcgccga cggcctgcac
     1681 tggaccttcg accttcgtcc gaacgtcaag ttccatagcg gcaacctcat gacgtgcaag
     1741 gacgtcgctt ggtcgttcaa gtacggcgcc ctcaccgcca acccggaagg cgcccccgcc
     1801 tacctgatgg gcaacctctg gctcggcacc gacatcgacg gctccgaccc ggcggccttc
     1861 ctcgacgcgg tcacgtggga catgatcgac ggcatcgtca cctgccccga aggcccgcaa
     1921 ggcctcaagg cgacggtcaa cctcgtcaac cagaccccgg ccctcatccc catcctcacc
     1981 tacacggcct tcagcatcgt cgactcgcag ttcgccatcg atggcggcgc gtgggacggc
     2041 accgaagcca cgtggaagga ctgggtcggc cgcgacctga ccgccgagtt cctccaccgc
     2101 aacgcgagcg gcaccggcgc gttcaagctc gtcgactgga ccgacgacag cgtcgtcgcc
     2161 gacgccttcg ccgactactg gggtggcgct cccgcgctca accacgtcat ctaccgctac
     2221 gtcgacgagc agtcgacccg catcctcgcc gtccagcagg gcgacgccga ccgcatcacg
     2281 cttaacgaga actccgccgt cgcccaggtc gaaggcgccg ccggtgtctc cgtcctccgc
     2341 gatcccaact ggctccccgc cagcgtcacc gcggccttct tcaacttcga catcgacgtg
     2401 accaacaacg aggacgtcgg ctccggcctc ctcgacggca agggcatccc gtccaacttc
     2461 ttcgccgacg tgaacgtccg ccgcggcttc gcccaactgt tcgaccagca ggcgttcgtc
     2521 gaccaggtct accagggcca gggcaccgtc atcacgatgg gtctgccctc cagcttcctc
     2581 ggttacaacc cggacgtcgc cgtccgcacc ctcgatctcg aagccgccac cgactacttc
     2641 cgcgccgcgt tcgacggtca ggtctgggag aagggcttcg agttcacggc cctctacaac
     2701 tcgggcaaca cgatccgcca gacggcgctt gaactcatca aggacaacct tgagttcatc
     2761 aacccgaagt tcaagatgaa cgtgcgcggt ctgccatggg ccgactacct ctcccgcacg
     2821 gccgagaaga aggccccgat gttcgccttg ggctgggctg ctgactacgc cgacccgagc
     2881 aacttcatca acaccttcta cgacgacgac ggctactacg cggcccgcac gagcatcagc
     2941 gtgcccgaga tccaggccat catcgatcag gccgatcagc tcgttgaccc cgaagcccgc
     3001 ggcttcctct accgcggcat cggcacgctc cactacgatc aggcgcccct catcgcggtg
     3061 ccgatccaga gcgcgttcct ggtggtccgt tccaacctga acggcgtgta ctacaacacg
     3121 atgtactccg acaagttcct ctggaaggac ctgtcgaaga actgagccgc cttctcgtgc
     3181 ggtggagcga ctttcatcga gtcgctccac cgcacgggcc agcaccgagg atcagccctc
     3241 cagtgtgcga cgtgcttcct gcgctggttc ggcggaatcg ccgagggtgc ggtgctggtc
     3301 gataagacgc ccctaacctt atggacccga tatcttgcta gtcttcgtcg ccaggcgcct
     3361 gctgttgctg ccggtcgtgt tcgttggggt cacgctggcc atcgtgttgc tgatgcagct
     3421 cctcacgcct tatcagcgcg ccgccgccta tgtccagtcc gagcagcaga tccgcaatat
     3481 cgattcgatc atcgtccagt acgggctcac cgacccctgg tacatgcagt acggacgctg
     3541 gatcggccag gtcatgaagg gcaacctcgg ctactcgcgc acctcgcgcg agcccgtgct
     3601 gaccacgctg ggtaaacgct tccccgtcac cctcgaactg gccatcctcg cgatcctgcc
     3661 cgtcatcggc gtcggcgtgg cgatgggcac ggccgcggcc ctcaaccgcg acaaacccat
     3721 agaccagttc gccagggtcg cgtcgatcat aggctggtcg ctgccgacgt tcgttctggg
     3781 gatatggttg ctggtcatct tctacgggtg gctgggatgg ttccaacccg gccgaatatc
     3841 tgacgccatc aggatctcaa cgggcccgaa ctccgggttc gtcaactaca cgcgcatgat
     3901 gctggtcgac gcgctcctca acaagcgcct cgacgtcttc tgggacgccc tcctgcatat
     3961 ggtcttgccg gtcgtcaccc tctcggtcgt gcagagcgcg cagataatgc gcgtcatgcg
     4021 ctcctcgctg ctcgacgcgc tcggtcggga ttacgtgcgc acggctcgcg ccaagggcct
     4081 tccggagcac acggtgaccc ataagcacgc gcggcgcaac gcgctcatcc cggtcatcac
     4141 cctctccggg ttcgtgctga tcgggctcat caacggcgtc gtcatcacgg agacgatctt
     4201 caactacccc gggctcgggc agtgggccgc cgcgtcggca gtcaacctcg actatgcctc
     4261 cgtgctcggc ttcgccgtgt tcaccgcgat gatggtggtc cttgccaacc ttctcgtcga
     4321 tatcctctac ggggtcgtcg acccacggat caggtacgag tagcatggcg gccgacctgc
     4381 ctgtcgccca gaacccggat ggcgccctcg acgcgcaccc cgaccagcct gcgcggaagg
     4441 gtagggcgtg gaagcgcttc cgccgcaacc ccctcgccgt cgtcggtctg atcatggtcg
     4501 cggccttcgt gctcatcgcc gtgttcgccc cggtcatcac ggagctgccg cgaagctgct
     4561 tgcgtgacct cgagctcacg gccgccaacc agcacgacta tcggaacccg accaagcctg
     4621 ctttctggaa agccgtcttc gccccgccga agtcgtgctt cacgatcgcg cgcgtgagct
     4681 tcagcacgca accggccgat gccgccacga ccaagaccgt cctcgggacc accagcggcg
     4741 gctacgacat cttctacggc ctcgtgtggg gcacgcgcac ggccttcaag gtcggcctca
     4801 tcgtcgtcgg catcgctttc gccatcgggg tcatcatcgg ctcgttgtcc ggctatctag
     4861 gcggcgtctt cgacaacctc gtcatgcgct tcaccgacat catcatctcg ttgccgagcc
     4921 tggtggtg
//
LOCUS       DAOQYE010000033         4908 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_17508_length_4908_cov_3.499691, whole genome shotgun sequence.
ACCESSION   DAOQYE010000033 DAOQYE010000000
VERSION     DAOQYE010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 4908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4908
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17508_length_4908_cov_3.499691"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1163)
                     /locus_tag="PKN52_01200"
     CDS             complement(<1..1163)
                     /locus_tag="PKN52_01200"
                     /inference="COORDINATES: protein motif:HMM:NF015647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HNQ98582.1"
                     /translation="MSGGMPAKPDLPPAIVVGVMSGTSLDGVDTVTARLERAGGTLKW
                     EVLARAAHSYPDDLVERLHLALKPETSDVLMLTELHQEVGRFYAEVVGAAQALHRVDL
                     VALSGQTVYHIPRVEPEHGWRVKSTLQLGEATVVTERCRVTTISDFRQSDFSAGGQGA
                     PLVSFPDSVLYAAPGIARAVVNLGGIANVTYLPASGDQNGVLAFDTGPANCLLNEAAA
                     RFAGEPFDQDGRLARAGTVDEAALARLLADPYFALTPPKTTGREHFHLDAALGRGWPD
                     GVPELPDLMATLAELTVGTLLGGIRDHLEPLGIDEVLVAGGGARNPVLMEGLRRGLEA
                     PVRGFAELGFDDKDRETLAMAVMGYYRVHGEPNVLPSATGAKWPVVAGKVAQP"
     gene            complement(1160..2263)
                     /locus_tag="PKN52_01205"
     CDS             complement(1160..2263)
                     /locus_tag="PKN52_01205"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNQ98583.1"
                     /translation="MPRTRTEPVGAAERAVAACAPLIEQAVAERVTPGAVLQVAGRDG
                     HGAVIARGAVAYGGAAVSASTRYDLASLTKVTACLPSLLSLVQAGEVALSDRVERFFS
                     NAGWMQEPSLGAVTILDLVLHRSGLPAWRPLFALTNDRRVAVANALQSSLTEPAGAHL
                     YSDIGVIVLTAIIERVTGKRIDEYAEEVVFAPLGMSNTGYGPLVTEGGVAPDVAATED
                     DGLRGLLSGRVHDENADALGGVSGHAGLFGPAGDLTRYAQAWLRLEAPFAGADVLRST
                     IIDLSGGEGPRRGVLWRCADADWPFGPGPSPQAYGHTGFTGTSIVIDPGRGWAVVLLT
                     NRVHPRRGSSEGVMRLRRAVHAAVAEAFAGEGA"
     gene            2464..3930
                     /locus_tag="PKN52_01210"
     CDS             2464..3930
                     /locus_tag="PKN52_01210"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNQ98584.1"
                     /translation="MVSLGTAFAQQGRLEVAIDTAPVGLDPHIVTAFSSFAVIGQIYD
                     GLLEVNAGLELEPALATSWTVSDDGLTYVFQLRDGVTFHNGRAFTADDVVYSFDRIMN
                     PETGSPQASRFNEVASAVATGPLEVTFTLNVPFAPFLSNLTNLTVVPREVVEANGGSL
                     QQVAVGTGPFMLKEIVPDTYVLLVANPNYYRQGEPKVAEVRYNIVPEASTRAAGIRTG
                     TYHIIPDVDPATAETLKNVRGVTLMGVQDLSYTLLGLNVSRAPFNDPRVREAINYAID
                     RAEIVEAVYFGNAQPGGPLSPGLTAWATDTANFPCYASNPDKARELLAQAGYPNGFHA
                     GLITFGTIQVVSDVAQVLQAQLARVGIVLDVNVAEFGAFVQAWRNSDFDTFVSLNGGA
                     TDPDGYLFRTFVTGGSTNVFKFSDPAVDELLQLGRTTTAFEDRVNIYDHLQKRLACTG
                     PISHVAYGTLFSAVTDNVVGFQQLPSRGLRYLREVELR"
     gene            3942..4892
                     /locus_tag="PKN52_01215"
     CDS             3942..4892
                     /locus_tag="PKN52_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ98585.1"
                     /translation="MFQYTLRRLLDLVFVLLGVSVLVFLMVRFIPGDAVAIMLGANTD
                     ITPERVDALRRQIGLDLPVYEQYWNWLTSALRGDLGTSIWTGTPVSQEILARLPATFE
                     ITFLALVLALVLSFPLGVLAARARGTSGDVVVRVIAIIGLTLPSFWVGVLLLYFFSIY
                     LPAWPAIGYVPFTSDPWGNLARVALPTVAVSLPMVAGLTRILRSSLLEVLSSDYVRTA
                     RAKGVGERGMLYKHALRNAMIPMVTVIGVQLGYLLSGVVVIEQVFAIPGVGRLMVGAI
                     NERNYPLLQGVVLIVTGVFVLINLLVDLTYAWIDPRVEYS"
BASE COUNT          729 a         1802 c         1630 g          747 t
ORIGIN      
        1 cacggctgcg cgaccttgcc agcgacgaca ggccacttgg cgccggtagc ggagggcagg
       61 acgttgggct cgccgtgcac gcggtagtaa cccatgaccg ccattgcgag cgtctcgcga
      121 tccttgtcgt cgaaaccgag ctcggcgaac ccgcgcacgg gcgcctcgag gccgcgcctc
      181 agcccctcca tgagcaccgg gttgcgcgcg cccccgccgg ccacgagcac ctcgtcgatc
      241 cccagcggct ccaggtggtc gcggatcccg cccagcaggg tgccgacggt cagctctgcc
      301 agcgtagcca tcaggtcagg cagctcgggg acgccgtccg gccagccgcg tcccagggcg
      361 gcgtcgaggt ggaagtgttc gcggccggtg gtcttggggg gcgtgagcgc gaagtacggg
      421 tctgccagca gccgcgcgag cgccgcctcg tcgaccgtcc cggcccgcgc cagccgacca
      481 tcctggtcga acggctcgcc ggcgaacctc gcggcggcct cgttcagcag gcagttggcc
      541 gggcccgtgt cgaaggcgag cactccgttc tggtcgccgc tcgctggcag gtaggtcacg
      601 ttggcgatgc cgcccaggtt gacgaccgcg cgggcgatgc cgggtgcggc gtacaggacc
      661 gaatcgggga agctgaccag cggcgcgccc tggccgccgg ccgagaagtc cgactggcgg
      721 aagtccgaga tggtcgtcac gcggcagcgc tcggtcacga ccgttgcctc gccgagctgc
      781 agggtgctct tgacgcgcca gccgtgttcc ggctccacgc gggggatgtg atagacggtc
      841 tggccggaga gcgcgactag gtcgacccgg tgcagtgcct gggccgcccc gaccacctcg
      901 gcgtagaacc gcccgacctc ctggtgcagt tccgtcagca tgaggacgtc ggaggtctcc
      961 ggcttgagcg ccaggtgcag gcgctccaca aggtcgtccg ggtaggaatg cgccgcgcgg
     1021 gcgagcacct cccacttcag cgtgccgcca gcgcgctcca agcgcgcggt cacggtatcc
     1081 acgccatcga gggacgtgcc tgacatgacg ccaacgacga tggcaggcgg gaggtcgggc
     1141 ttggccggca tgccgccgct cacgcgccct cacccgcgaa cgcctcggcg acggcggcgt
     1201 ggacggcccg gcgcaagcgc atcaccccct cggacgaccc gcggcgcgga tgcacgcggt
     1261 tggtgaggag gacgacggcc cagccgcggc ccgggtcgat cacgatgctc gtgcccgtga
     1321 agcccgtgtg cccgtaagcc tggggggagg gtcctggacc gaacggccag tcggcgtcgg
     1381 cgcagcgcca caggacgccg cggcgcggcc cctcgccgcc cgagaggtcg atgatggtcg
     1441 agcgcagcac gtcggccccc gcgaacggcg cctccaagcg caaccacgcc tgggcgtagc
     1501 gcgtcaggtc gccggccggg ccgaagaggc cggcgtggcc ggacacgccg ccgagcgcgt
     1561 cggcgttctc gtcgtggacg cggcctgaca agaggccccg gagtccgtcg tcttcggtgg
     1621 cggcgacgtc gggcgcgact cccccttccg tgaccagcgg cccgtagccg gtgttggaca
     1681 tcccgagagg ggcgaagacg acctcttcgg cgtactcgtc gatccgcttc cccgtcacgc
     1741 gctcgatgat ggcggtgagg acgatgacgc cgatgtcgga gtagaggtgc gcgccggccg
     1801 gttcggtgag gctcgactgc aacgcgttcg cgacggcgac gcgccggtcg ttcgtgaggg
     1861 cgaacagagg tcgccaggcc ggcagtccgg agcggtgcag caccaggtcg aggatggtga
     1921 cggccccgag cgacggctct tgcatccagc cggcgttgct gaagaagcgc tccacccggt
     1981 cgctcagcgc cacctcgccc gcctgcacga ggctgaggag gctcggcagg catgccgtca
     2041 ccttcgtgag cgacgcgagg tcgtagcgcg ttgacgcgct cacggccgcg ccgccgtacg
     2101 ccacggcgcc gcgcgcgatg acggcgccat gtccgtcgcg gcccgctacc tgcaggaccg
     2161 ctcccggagt cactcgctcg gctacggcct gctcgatgag cggcgcgcac gccgcgacgg
     2221 cgcgctcggc cgccccgacc ggctcggtcc tggtcctagg catggtgaag gctacaccat
     2281 agccaccgac tggtctagca atggtacgtt aggtggcgta agcgctcatc accgccgccg
     2341 caacgcgcgg gccggagccg acccaacgac gttgtcgggc cgaccggacc acttcagccg
     2401 cgagagcgct gaccagggaa ggaagggaac atgcgcgcag caaggcttct cctaatagca
     2461 gccgtggttt cgctaggcac ggcgttcgcc cagcagggaa ggctcgaggt cgccatcgac
     2521 acggcgccgg tcggcctcga cccacatatc gtcacggcgt tctcgtcgtt cgcagtcatc
     2581 ggccagatct acgacggcct cctggaggtc aacgccggcc tcgagctcga accggccctg
     2641 gccacgtcct ggacggtgtc agacgacggc ctcacctacg tcttccagct cagggacggg
     2701 gtcaccttcc acaacggccg cgccttcact gccgacgacg tcgtctactc gttcgaccgc
     2761 atcatgaacc cggagaccgg ctcgccgcag gccagccgct tcaacgaggt cgcctcagcc
     2821 gtcgccacgg gcccgctaga ggtcacgttc actctcaacg tgcccttcgc cccgttcctg
     2881 tccaacctca cgaacctgac ggtggtgcca cgcgaggtcg tcgaggccaa cggtgggagc
     2941 ctacagcagg tagccgtcgg caccggaccg ttcatgctga aggagatcgt ccccgacacg
     3001 tacgtgctgc tcgtggcgaa cccgaactac taccgccagg gcgagccgaa ggtggccgag
     3061 gtccgctaca acatcgtccc agaggcctcg acgcgcgccg ccggcatccg cacgggcacg
     3121 taccacatca tcccggacgt ggacccggcc accgccgaga cgctcaagaa cgtccgtggc
     3181 gtcaccctca tgggcgtcca ggacctctcc tacacgctcc tcggcctcaa cgtgagccgc
     3241 gcgccgttca acgacccgcg cgtgcgcgag gccatcaact acgccatcga ccgcgccgag
     3301 atcgtggagg ccgtctactt cggcaacgcc caaccgggcg gacccctgag ccccggtctc
     3361 actgcctggg cgaccgacac ggccaacttc ccctgctacg cctccaaccc ggacaaggcc
     3421 cgcgagctgc tcgcccaggc cggctacccc aacggcttcc acgccggcct catcacgttc
     3481 ggcaccatcc aggtcgtcag cgacgtcgcc caggtcctgc aggcgcagct ggcgcgggtc
     3541 ggcatcgtcc tcgacgtcaa cgtcgccgag ttcggcgcgt tcgtgcaggc gtggcgcaac
     3601 tccgacttcg acaccttcgt gagcctcaac ggcggcgcca ccgacccgga cggctacctc
     3661 ttccgcacct tcgtgacggg cggctcgacg aatgtcttca agttctccga cccggccgtc
     3721 gacgagctgc tgcagctcgg ccgtaccacg accgcgttcg aggaccgcgt caacatctac
     3781 gaccacctgc agaagcgcct cgcctgcacg ggccccatca gccacgtggc gtacggcacg
     3841 ctcttcagcg cggtcaccga caacgtcgtc ggcttccagc agctcccgag ccgcggcctg
     3901 cgctacctgc gcgaggtgga actccgctaa gggtctgacg cgtgttccag tacacgctcc
     3961 ggcgcctgct cgacctggtg ttcgtgctcc tcggggtctc ggtcctcgtc ttcctgatgg
     4021 tgaggttcat ccccggcgac gcggtcgcca tcatgctggg cgccaacacc gacataacgc
     4081 ctgagcgcgt cgacgcgctc aggcgtcaga tcggtctcga cctgcccgtc tacgagcagt
     4141 actggaactg gttgacgagc gccctgcggg gcgacctcgg cacgtccatc tggacgggca
     4201 cgcccgtgag ccaggagatc ctcgcgcggc tgcccgccac gttcgagatc actttcctcg
     4261 ccctcgtcct cgctctcgtc ctgtcgttcc cgctcggcgt cctcgccgcg cgggcgcggg
     4321 gcacgagcgg ggacgtggtg gtgagggtca tagccatcat cggcctcacg ctgccgtcgt
     4381 tctgggtcgg cgtgctcctc ctctacttct tctccatcta cctcccagcc tggccggcca
     4441 tcggctacgt cccgttcacg tcggacccct ggggcaacct cgcgcgcgtg gcgctgccaa
     4501 cggtggccgt gagcctcccc atggtggccg ggctgacgcg catcctgcgt tcgagcctcc
     4561 tcgaggtcct ctcgagcgac tacgtgcgca cggcacgcgc caagggggtg ggcgagcgcg
     4621 gcatgctcta caagcacgcc ctgcgcaacg ccatgatccc gatggtcacg gtcatcgggg
     4681 tgcaactcgg ctacctgctc tccggcgtcg tcgtcatcga gcaggtcttc gccatccccg
     4741 gggtcggccg cctcatggtc ggggccatca acgagcgcaa ctacccgctc ctgcaaggcg
     4801 tcgtgctgat cgtcaccggg gtgttcgtgc tcatcaacct gctggtcgac cttacctacg
     4861 cctggatcga cccgcgcgtg gagtactcgt gagcctgcgc cgcttctt
//
LOCUS       DAOQYE010000034         4886 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_17613_length_4886_cov_2.620575, whole genome shotgun sequence.
ACCESSION   DAOQYE010000034 DAOQYE010000000
VERSION     DAOQYE010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 4886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4886
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17613_length_4886_cov_2.620575"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            215..694
                     /locus_tag="PKN52_01220"
     CDS             215..694
                     /locus_tag="PKN52_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98586.1"
                     /translation="MSTTRSLTLDVQPPPVNPYPRVTGYGVYSRTLTSGPLYLCLNAN
                     VPAGNTIDFTENGCNFVGETGTHRRYSAFVQVENPDNEALTYDWRVYVTYGGSEHLIN
                     GVSGSTDNTFVPYSPGNALLGTEACRIAVTVHAPDPTRSKSLTVWTGSCTYYSTYIG"
     assembly_gap    919..928
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <929..2331
                     /locus_tag="PKN52_01225"
     CDS             <929..2331
                     /locus_tag="PKN52_01225"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HNQ98587.1"
                     /translation="VSLSLRAVAKSYGGTTVFTDVSITVGRGEKVALIGPNGGGKSTL
                     LRVAAGLEQPDAGSVLAQGRLAYLEQAPGAPSELSGGQARKAEIAERLLEEADVYLLD
                     EPTNHLDAAGLAWLERWVRSSPATMLIVSHDRAFLDATVTAVAELERGELTTWPGTYT
                     EAMAEKATVRSARFRQHEAQVRTKRRLEQEAAKLASKGKSADKFNKRRAGNQALILAK
                     AKAENVSRTLARRAKALQERAEKLEPVQKPFEAAGLPEIPLPDVTLGPTDVVRFKGVD
                     LARGGKTLIRDLDLVVRRGEKLALVGPNGSGKSSLLALVTGELLPTAGTVVANVRPFA
                     LSQHGTELDAFATLEEALLDAQGALRRQDLHYLLARMGLPPNPAAPVATLSGGQRTRL
                     ALARLSVTRSPLLVLDEPTNHLDVGMVEALERLLAAYPGAILLASHDRRLVAAVAGRT
                     LRPGGDGAWTVSEGDG"
     gene            complement(2342..>4886)
                     /locus_tag="PKN52_01230"
     CDS             complement(2342..>4886)
                     /locus_tag="PKN52_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNQ98588.1"
                     /translation="VAHAKGGSAAAAAAVEARALLPAGEPNLRVWGEVAGLPHGELAA
                     QAARLHRLGLLRGGDFSHGLYRELTLATLPADRRKSMAARAVASLDSDPVAAAHFVDG
                     AGLPATAARDLLLAAAAAAPYKVDKARFLARASRHAQGGERARLAYEAAMQLIDFDLP
                     AAFELLEQAAADPGAEPRMVRAYAHALARQGRLDAVRELVARLAADGVPERELADLLV
                     TSFNAAGDHRSALEVWKANAGLHADADFGLLRAAAASALASGDMGLAEELLDRGTSGD
                     VDAVLRCEFISLRALAAYHRGDYVASDELISGAIGLLRGLDRPRILSTALLNRAAFLK
                     QLCRFTEMNDSLEECLRLRSETLDGRSYAFAQAALAELRIDQARFAEAEDLLDEALET
                     QELYGPSRFLVNTLSMRSVLHLARGEPLLAWHYAERSLQLARETGGPRVVLEILFDAA
                     AARARMGEGSKALALAEEMVALGPETGASPLNECRSRWARGLALAAAGDVATAALELR
                     AALEVARAGGLALEASKVAIDLATTEVDDTAAREALAWFDANGLAIGRVLVGRAFPGL
                     VDADSSPSELPGPARPTAGQGMGTAAAPGGDSGLRLEVLGDMRLGPVGGTEAVKGERR
                     RLLLARLLQTRMDGGAEVTDLELIEALYPGMPEPDAVRALKQLVHQVRRKYGTRVLIR
                     THAGYALGAAVGSDAEGFLAGAGSGGTVGADAGVTSEHGRDQAADDPAAPLAVWRGPF
                     LKDVPEAREDGVTARLTTAAARLASRAVGSSPADVVRVAQYLQDVAPFEAHLAALEVA
                     ARERLGDQAGAAAALERARRRLAEVGEALDTGEMACLKARLEARPEMGS"
BASE COUNT          753 a         1763 c         1686 g          674 t
ORIGIN      
        1 gggcgccagc ggcatcaggc gcgcataagg cttgcccgcc ttggcgagga tgatctcctg
       61 tccggcatgg gcctgctcga gcaggcgaga caattgcgtc ttggcctcat ggacattgac
      121 ggtgagcgcc atgactttct ccaatcgaac taagtcactg gaccaagtct agcttaaaac
      181 gtttcatcgg cgtgactgct ggattcagaa cggcttgagc acgacgagga gcctcacgct
      241 cgacgtgcag ccgccgcccg tcaaccccta cccgcgcgtc accgggtacg gcgtctactc
      301 gcgcacgttg acctccggcc cgctctactt gtgcctcaac gccaacgtgc cagccggcaa
      361 caccatcgac ttcacggaga acggctgcaa cttcgtaggc gagacaggaa cgcataggcg
      421 ctactccgcc ttcgtccagg tggagaaccc cgacaacgag gcgctcacgt acgactggcg
      481 cgtatacgtc acctacggcg gatccgagca cctcatcaac ggcgtctctg gttcgaccga
      541 caacacgttc gttccgtact cgccaggcaa cgcgctgttg ggaacagagg cctgccggat
      601 cgccgtgacc gtccacgctc ccgatccgac gcgcagcaag tccctgacag tgtggacggg
      661 cagctgcacc tactactcca cctacatcgg ttgagtagag cttaggcggg gccgccggca
      721 acgaggttcc gggcgggtgg acagcgcgag actacgctgt ccacccgctc atcggtgcta
      781 agctccacgt gccgcgcgag gagttggccc gcgcggaccc ctgacgtcta gcggaaggtc
      841 ataccaggta gatgcgcggc ttgcactgac gacgagtact tgaacccaag tagttcctcg
      901 actccgatca ggaggtcann nnnnnnnngt gtgagtcttt cgttacgcgc cgtggccaag
      961 agctacggcg gcaccaccgt cttcacggac gtttccatca cagtcgggcg cggcgagaag
     1021 gtcgcgctca tcggtcccaa cggcggcggc aagtcgacgc tgctgagggt ggcggcgggt
     1081 ctggagcagc ccgacgccgg cagcgtactg gcgcagggcc gcctcgccta cctcgagcag
     1141 gcgccgggcg caccgagcga actctccggc ggccaggcgc ggaaggcgga gatcgccgag
     1201 cgcctcttgg aagaggccga cgtctacctg ctcgacgagc ccaccaacca cctcgacgct
     1261 gccgggctcg cgtggctcga gcggtgggtg cgctccagcc cggccaccat gctcatcgtc
     1321 tcgcacgacc gggccttcct cgacgcgacg gtgacggcgg tggcggagct cgagcggggc
     1381 gagctgacga cgtggccagg cacctacacg gaggccatgg ccgagaaggc caccgtgcgg
     1441 tccgcgcggt tcaggcagca cgaggcgcag gtgcgcacga agcggaggtt ggagcaggag
     1501 gccgccaaac tcgcctcgaa gggcaagagc gcggacaagt tcaacaagag gcgggcaggc
     1561 aaccaggcgc taatcctcgc gaaggcgaag gcagagaacg tgtcgcggac gctcgcccgg
     1621 cgcgccaagg cgctccagga gcgcgccgag aagctcgagc ccgtgcagaa gccgttcgag
     1681 gccgctggcc tgcccgagat cccgctgcct gacgtgacac tgggtccgac ggacgtcgtg
     1741 cgcttcaaag gagtcgacct ggcgcgcggc ggcaagacgt tgatacgcga cctcgacctg
     1801 gtggtccgcc gcggcgagaa gctcgcgctc gtcggtccga acggcagcgg caagagcagc
     1861 ctgctggcgc tcgttaccgg cgagctactg ccgacggccg gcaccgtcgt cgccaacgtg
     1921 cgaccgttcg ccctcagcca gcatggcacc gagctggacg ccttcgccac gctcgaggag
     1981 gcgctcctgg acgcccaagg ggcgctgcgg cgccaggacc tccactacct actggcgcgc
     2041 atgggcctgc cgccgaaccc ggcggcgccc gtcgccacgc tctccggcgg ccaacggacc
     2101 cgcctcgcgc tcgcacgcct gagcgtcacc cggtcgccgt tgctcgtact cgacgaaccg
     2161 acgaaccacc tcgacgtcgg catggtcgag gcgttggagc ggctgctcgc ggcgtaccct
     2221 ggcgcgatcc tgctggcgtc gcacgaccgt cgcctcgtcg cggcggtggc gggccgcacg
     2281 ctgcggccgg gcggcgacgg cgcctggacc gtctccgaag gcgacggcta ggccgctcgt
     2341 cctaggagcc catctccggg cgtgcctcaa ggcgcgcctt gaggcacgcc atttcgccgg
     2401 tgtccagcgc ctcgccgacc tccgccaggc gccgccgcgc cctttccagc gccgcggctg
     2461 cgccggcctg gtcgccgagg cgctcgcgcg ccgcgacctc gagggcggcc aggtgagcct
     2521 cgaaaggagc cacgtcttgc aagtactgcg ccacgcgcac gacgtctgcg gggctagatc
     2581 caacggcccg gctcgccagt cgggccgccg ccgtcgtaag gcgagcggtc acgccgtcct
     2641 ctcgagcctc gggaacgtcc ttcaggaacg ggccgcgcca cacggccaac ggcgcggcgg
     2701 ggtcgtcggc cgcctggtca cgcccgtgct ccgaggtgac accggcgtcg gcgccgactg
     2761 ttccgcccga cccggcgccg gcgaggaacc cctcggcgtc cgagccaacg gccgcgccga
     2821 gcgcgtaacc cgcgtgagtg cggatgagga ctcgcgtacc gtacttgcgt cgcacctggt
     2881 gcacgagctg cttcagcgcc ctcacggcgt ccggctccgg catgcccgga tagagggcct
     2941 cgatgagctc caggtccgtc acctcggcgc cgccgtccat gcgtgtctgc aggaggcgcg
     3001 ccaataggag gcgacgtcgc tcgcccttga ccgcctccgt cccgccgacg ggtccgagcc
     3061 gcatgtcgcc aagcacctca agccgcagtc ccgagtcgcc tccgggcgcg gccgctgtcc
     3121 ccatcccctg gccggcggta ggccgcgccg ggccgggcag ttcgcttggg gaggagtcgg
     3181 cgtcgaccag accggggaag gcgcgcccga cgagcacgcg cccgatggca aggccgttgg
     3241 cgtcgaacca cgccaaggcc tccctcgcgg ccgtgtcgtc gacttccgtg gtggcaaggt
     3301 cgatagcgac cttgcttgcc tcgagcgcca gcccgccggc ccgcgccact tccagcgcgg
     3361 cgcggagctc aagggcggcg gtcgcgacgt cgccggcggc ggccagcgcc aggcccctgg
     3421 cccaccgact gcggcattcg ttcagaggcg aggcgccggt ctcgggacca agggcgacca
     3481 tctcctccgc cagcgccagc gccttggagc cttcgcccat gcgggctctg gccgcggcgg
     3541 cgtcgaacag gatctcgagc accacgcgcg gcccgcccgt ctcgcgagca agctggagcg
     3601 accgctcggc gtagtgccag gcgaggagcg gctcgccgcg cgccaagtgc agcacggacc
     3661 gcatggagag cgtgttcact aggaagcgcg atgggccgta cagttcctgc gtctccagcg
     3721 cttcgtcgag gaggtcctcc gcctccgcga agcgtgcctg gtcgatgcgc agctccgcca
     3781 gcgccgcctg cgcgaacgcg tatgagcgac cgtcgagcgt ctcgctccgg aggcgcaggc
     3841 actcctccag agagtcgttc atctccgtga agcggcagag ctgcttgagg aacgcggcgc
     3901 ggttcaggag agcagtgctg aggatccggg gcctgtccaa gccccttagc aacccgatcg
     3961 ccccgctgat gagctcgtcg ctggcgacgt aatcgccccg gtggtaggcc gccagcgccc
     4021 gcaacgagat gaactcgcag cgcaggacgg cgtcgacgtc gccgctggtg ccacggtcga
     4081 gcagctcctc cgcgagaccc atgtcgccgc tggccaaggc cgatgcggcg gccgcgcgta
     4141 gaagcccgaa gtcggcatcg gcatgcaggc cggcgttggc cttccatacc tccagcgcgg
     4201 agcggtgatc gccggcggcg ttgaagctgg tgacgagcag gtcggcgagc tcccgctccg
     4261 gcaccccgtc ggccgccaac ctggctacca gctcgcggac cgcgtctagc ctgccctgac
     4321 gcgcgagcgc gtgggcgtag gcccgcacca tgcgcggctc ggctccgggg tcggcggctg
     4381 cctgctcgag cagctcgaac gcggccggca ggtcgaagtc gatgagctgc atggcggcct
     4441 cgtaagcgag acgcgctcgc tcgccccctt gcgcatgccg cgaggctcgc gccaagaacc
     4501 tggccttgtc gaccttgtac ggggccgcgg ccgcggccgc gaggagcaag tcgcgcgccg
     4561 cggtggcagg caggccggcc ccgtcgacga agtgcgccgc ggcgacgggg tcggagtcga
     4621 ggctggcgac ggcgcgtgcc gccatggact tgcgtctgtc ggccggcaga gtcgcaagcg
     4681 tgagctctcg atagagcccg tgcgagaagt cgccacccct caggagcccg agcctatgca
     4741 accgagccgc ttgcgcggca agctcgccat gcggcaggcc ggcgacctcg ccccagacgc
     4801 gcaggttcgg ctcgccggcc ggcagtaggg cccgcgcctc cacggccgcg gcggccgcgg
     4861 ccgaaccgcc cttcgcgtgc gccacc
//
LOCUS       DAOQYE010000035         4867 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_17693_length_4867_cov_2.160848, whole genome shotgun sequence.
ACCESSION   DAOQYE010000035 DAOQYE010000000
VERSION     DAOQYE010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 4867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4867
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17693_length_4867_cov_2.160848"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="PKN52_01235"
     CDS             <1..237
                     /locus_tag="PKN52_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98589.1"
                     /translation="ADGLDTMSVPTFDPSVAARAASAASLYDGLPLGLTDATLVVHTQ
                     DYRTRDLFTVDQRHFRAVRPLWGGDSFRLLPLDA"
     gene            complement(259..909)
                     /locus_tag="PKN52_01240"
     CDS             complement(259..909)
                     /locus_tag="PKN52_01240"
                     /inference="COORDINATES: protein motif:HMM:TIGR02453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HNQ98590.1"
                     /translation="MTTSQTFDGFHPDALPFFRELRENNERTFWLANKPRYDEHVKAP
                     MVALGGALEGAFGEPHLFRPYRDVRFSKDKRPYKLRVSMVFGGNGPMTVGSRYVQLDS
                     AGLYAGGGAYMLAPEALARFRVAVSEERSGTELVEVLTELEQGGYAVEGETLQRPPRG
                     FEADHPRIRFLKHKGLYVGRAFAPADWLYRPEALDRVAKVFGDVEPLVVWLRRHVG"
     gene            complement(906..1100)
                     /locus_tag="PKN52_01245"
     CDS             complement(906..1100)
                     /locus_tag="PKN52_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98591.1"
                     /translation="MSLRRQTLLSAIATSGGFLWVFVAAATSASAMIMWLGVGVLVVG
                     AAWAVRTRIRTMLSGDQESA"
     gene            1213..1890
                     /locus_tag="PKN52_01250"
     CDS             1213..1890
                     /locus_tag="PKN52_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HNQ98592.1"
                     /translation="MAACRLCPRLVEYRERVAREKRRAYHDAEYWGAPVPGFGDPDAK
                     LVLVGLAPGAHGSNRTGRMFTGDASGDFLYPALHRAGLASQPKATARDDGLTLSGVWI
                     TAAMRCVPPGNKPERSELVNCLGWLAHDLDSLRSARVALAIGKIGHDAAISLWRSRGL
                     RTTLAAHPFAHGAVHELGEVPGGPESGALTLVDTYHVSFQNTNTGRLTPAMFDAVIAK
                     AKELARL"
     gene            complement(1900..2655)
                     /locus_tag="PKN52_01255"
     CDS             complement(1900..2655)
                     /locus_tag="PKN52_01255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNQ98593.1"
                     /translation="MRTKESANIGAAARAMFNFGLHDLWLVAPRCRVDHRSYDLATHA
                     EPVLKAVTQVGTLDEALADVTLAFGTSARPRKAENYRVATPREAAAELGGGTAVVFGP
                     EDHGLANEELTRCQAQIVIPTVDFASLNLAQAVLVVAYEYAQAGRRATLPVTGGAVDP
                     EDARHRAASRDQLERFYAQLEETMLKIGYTDERRAPGMLRVYRGLLDRAEPTAHEVAA
                     FRGLLSQVAWAARQQPEPPPERFAAPASPQEDP"
     gene            complement(2971..3249)
                     /gene="rpsT"
                     /locus_tag="PKN52_01260"
     CDS             complement(2971..3249)
                     /gene="rpsT"
                     /locus_tag="PKN52_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HNQ98594.1"
                     /translation="MSQNASAMKRHRQSEERRLANRAKKSTIRTFTKKAVAAAETGDF
                     AAAAKYQKVVQGLVDRAIKSSTLHANTAARRKSRLAKRINSLQQGKAQ"
     gene            3475..4173
                     /locus_tag="PKN52_01265"
     CDS             3475..4173
                     /locus_tag="PKN52_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98595.1"
                     /translation="MLDLGTSIALIRDTLLLQPGTTELLRLGGNALRLAFYVLLAAGL
                     SEAVGESVVLFVNRVSPRRFAISLLISAFLFAFGYLFFALSVYLLARYALRIEVPFSF
                     VAGIVALGQAPRLFGYLAFIPYLGLPISVLLWTWSVIATIVGVAEGLSLDPLEALLTV
                     AFGALILVSAQRTVGRPLIALARLTRRRAAGVPLVTDRRQWLELVDSAPEPGLAEPRA
                     RTVRRANRKTGPTA"
     gene            4170..>4867
                     /locus_tag="PKN52_01270"
     CDS             4170..>4867
                     /locus_tag="PKN52_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98596.1"
                     /translation="MITLLIAVAIGALTVSLLLSPIETLGWWAGWYGEGLEHPHEASH
                     AVYRRIGDDKEPRQFVVYLDGIAKVGAENYDDVQGLLDRLNAALPRTVILGDLMPYSV
                     RNVGLLDERPLARFWRRMFQYKLAGSHSLLTFSINIRNLFQVLVAADPRYGSVYGRGE
                     AQVMLTSLLRAGYVPGSRVAITILGYSGGVEIGLVAARFLKRALDAPLTVVSLAGVMA
                     SDAGLEAIDRLYHL"
BASE COUNT          697 a         1740 c         1621 g          809 t
ORIGIN      
        1 gccgacggcc tggacaccat gtcggtaccc actttcgacc caagcgttgc tgcacgggcg
       61 gccagcgccg ctagcctgta cgacggacta ccgctcggcc tgacagacgc gaccctcgtc
      121 gtccacacgc aggactaccg aacgcgcgac ctgttcacgg tcgaccaacg ccacttccgg
      181 gctgtcagac cgctatgggg cggcgatagc tttcgcttac tgcccctgga cgcgtagcaa
      241 gccgcccttt cgtcaccctc agcccacatg tcgccgcaac cacaccacca gcggctccac
      301 gtcgccgaac accttggcaa cgcggtcaag agcctccggc cggtacagcc agtcggccgg
      361 ggcgaaggcg cggccgacgt agagcccctt gtgcttgaga aagcggatgc gcgggtggtc
      421 cgcctcgaag ccccgcggtg gccgctgcag ggtctcgccc tcgacggcgt agccgccttg
      481 ctcgagctcc gtgaggacct cgaccagttc cgtgccgctg cgctcctcgc tcacggcgac
      541 ccggaaacgc gccaaggcct ccggcgccag catgtaggca ccgccaccgg catagagccc
      601 agcgctgtcg agctgcacgt agcgcgaccc tacggtcatc ggaccattgc cgccgaagac
      661 catggagacc cgcagcttgt aaggccgctt gtccttgctg aagcgcacgt cgcggtaagg
      721 ccggaagagg tggggctcgc cgaaagcacc ctccagggcg cctccaagcg ccaccatggg
      781 tgccttgacg tgctcgtcgt accgaggctt gttcgccagc cagaacgtgc gctcgttgtt
      841 ctctcgcagc tcgcggaaga acggcagcgc gtccggatgg aacccatcga aggtctggga
      901 ggtggtcatg ccgactcctg atcaccgctc agcatggtcc tgatgcgcgt tctgaccgcc
      961 cacgccgcgc cgacgaccag tacccctacg cccagccaca tgatcatcgc cgaggccgag
     1021 gtcgcggccg ccacgaagac ccagaggaag ccgccggagg tcgcgatcgc ggagagaagc
     1081 gtctgacgcc tgaggctcat atcgaccgtt ccaacgctac cacccgctca agggcgttac
     1141 cctgcctgga tgagtccgcc gagcgcagac ccgtctcctg gtactccagc tttcgacccg
     1201 taccctcctg ccttggcagc atgccgccta tgccctcggc tggtcgagta ccgcgagcgc
     1261 gtcgcgaggg agaagcgccg tgcctaccac gacgcagagt actggggcgc gcccgtgccg
     1321 gggttcggcg acccagacgc caagctcgtg ctcgtgggtc tcgcaccggg cgcgcacggg
     1381 agcaaccgca cggggcgcat gttcaccggc gacgcgtcgg gcgacttcct ctaccccgcc
     1441 ctgcaccggg cggggctggc gtcgcagccg aaggccaccg cgcgagacga cgggctcacg
     1501 ctctcgggcg tctggatcac ggcggccatg cgctgcgtgc cgcccggcaa caagccggaa
     1561 cgtagcgagt tggtcaactg cctgggctgg ctcgcccacg acctggatag cctgcgctcg
     1621 gcccgggtcg ccttggcgat cggcaagatc gggcacgacg ccgccatctc cctatggcgc
     1681 tcacgtggtc tgaggacgac cctggccgcc cacccgttcg cccacggcgc cgtgcatgaa
     1741 ctcggcgagg tgccgggcgg gccggagagc ggcgcactca cgctcgtaga cacctatcac
     1801 gtcagcttcc agaacacgaa cacgggaagg ctcacgccgg ccatgttcga tgccgtgatc
     1861 gccaaggcga aggaactggc caggctctag agcgcaggct caggggtctt cctgcggcga
     1921 cgcgggcgcg gcgaaccgct ccggcggcgg ctccggctgt tgccgcgccg cccaggcgac
     1981 ctggctgagc agcccgcgga aggccgcgac ctcgtgcgcg gtcggctcgg cccggtcgag
     2041 gagcccgcga tagacgcgca gcatgccggg cgcgcggcgc tcgtccgtat agccgatctt
     2101 caacatggtt tcctcgagct gcgcgtagaa acgctcgagc tggtcgcggc tggccgcccg
     2161 gtgcctagcg tcttccgggt cgacggctcc accggtcacg ggtagggtgg cgcgccgacc
     2221 agcctgcgcg tactcgtacg cgacgaccag gacggcctgg gcaaggttga gcgaggcgaa
     2281 gtcgacggtc gggatcacga tctgcgcctg gcaacgtgtg agctcttcgt tggcgagccc
     2341 atggtcttcc ggaccgaaga cgacggccgt cccgccgcct agttcggctg cggcctcgcg
     2401 cggcgtggcg acccggtagt tctctgcctt gcgcggccga gcgctcgtcc cgaacgccag
     2461 ggtgacgtcg gcgagggcct cgtccagcgt cccgacctgc gtgaccgcct tcagcacggg
     2521 ttcggcgtgg gtcgccaggt cgtatgaccg gtggtcgacg cggcagcgtg gcgccacgag
     2581 ccacaggtcg tgcagcccga agttgaacat ggccctcgcc gccgcgccga tgttggcaga
     2641 ctccttcgtg cgcacgagca ccacccttgg gccgctcgta ggcccgcccc ttgggtcccc
     2701 tcggctaccg gtgctctcgg aaggtcctgc gttcatccgg ccagcatacc gaggcgcacg
     2761 ggagaaggtc cgtccgctcc gggcagcggc ggccccggcg tggggtcggg gacggcgcgc
     2821 cgtctggcgc gccggacggc gccgcaccac cagggcgccg acacgccaaa gccgacacgc
     2881 caaacgagag ccggcggacg ccaggtccgc cggctcggca cgaacagccc gcgaagggcc
     2941 ttcggcctga tgctcgggct ttattgggct ttactgggcc ttgccttgct gcagcgagtt
     3001 gatgcgcttg gcgaggcgcg acttgcggcg agcagccgtg ttggcgtgca gggtggagga
     3061 cttgatggcg cggtccacga ggccttgtac gaccttctgg tacttggcgg cggcggcgaa
     3121 gtcaccggtc tccgcagcgg cgacggcctt cttcgtgaag gtacggatgg tgctcttctt
     3181 ggcccggttg gcgaggcgcc gttcttcgga ctggcggtgg cgcttcatcg cggatgcgtt
     3241 ctgtgacacg ttgctcctcg ttcttccctt tacgcatggg ctgtgtcggc cctgcctggt
     3301 gcggtggaat cttttggccc cgtctggggc cgacgccctc ggtctcgggt cgggaacgga
     3361 ccacgtccgt aacggaccgt cggctcgatg cgccgatagc ctggcgatgc tagcacatcg
     3421 acgtggtctg ctcgaggagc gcgtgaagcg tcttggcctg ttagcgtcta ggtgatgctc
     3481 gacctgggga cctcgatcgc gctcatccgt gacaccctcc tcctgcagcc gggcaccacc
     3541 gagctcctgc gtctgggcgg caacgcgctg cggctggcct tctacgtcct gctcgccgcc
     3601 gggctctccg aagccgtagg cgagagcgtc gtgctcttcg tcaaccgcgt gtcgccgcgg
     3661 cgcttcgcga tctcgctcct catctctgcc ttcctcttcg cgttcggcta cctgttcttc
     3721 gcgctctccg tctacctgtt ggccaggtac gcgctgcgca tcgaggtgcc gttctcgttc
     3781 gtggcgggca tcgtcgcgct cggccaggcg ccgcgcctct tcggctacct tgccttcatc
     3841 ccctacctcg gcctgcccat ctccgtcctg ctctggacgt ggagcgtgat cgccacgatc
     3901 gtcggggtcg cggaaggctt gagcctcgac cccttggagg cgctgctcac ggtagcgttc
     3961 ggcgcgctca tcttggtgag cgcgcagcgc acggtggggc ggccgctcat cgccctcgcc
     4021 aggttgacgc gcaggcgcgc ggccggcgtg ccgctggtga ccgatcggcg acagtggctg
     4081 gaactcgtcg actcggcgcc ggaacccggc ctcgcagaac cccgagcgcg gaccgtccgc
     4141 cgggcgaacc gcaagaccgg gccgacggca tgatcacgct cctcatcgcc gtggccatcg
     4201 gggcgctgac cgtctcgctc ctcctgtccc ccatcgagac gctcgggtgg tgggcgggct
     4261 ggtacggcga aggcctggag caccctcacg aggcgagcca cgccgtgtac cggaggatcg
     4321 gcgacgacaa ggagccgcgc cagttcgtgg tctacctgga cggcatagcc aaggtcggcg
     4381 ccgagaacta cgatgacgtc caaggcctcc tcgacaggct gaacgccgct ctgccgcgca
     4441 ccgtcatcct cggcgacctc atgccgtact ccgtgcgcaa cgtcggactg cttgacgagc
     4501 gacccctcgc taggttctgg cgacggatgt tccagtacaa gctcgccggt agccactcgc
     4561 tcctcacctt cagcatcaac atccgtaacc tcttccaagt gctcgtggcg gcggacccgc
     4621 gttacggctc cgtctacggc cggggcgagg cccaggtgat gcttacctcg ctcctgcgcg
     4681 ccggctacgt gcccggtagt cgtgtcgcca tcaccatcct cggctacagc ggcggcgtcg
     4741 agatcggcct cgtcgccgcg cgcttcctca agcgcgccct cgacgcgccc ctcaccgtcg
     4801 tctcgctggc gggcgtgatg gcgagcgacg ccgggttgga ggccatcgac cgcctgtacc
     4861 acctgga
//
LOCUS       DAOQYE010000036         4854 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_17732_length_4854_cov_3.410502, whole genome shotgun sequence.
ACCESSION   DAOQYE010000036 DAOQYE010000000
VERSION     DAOQYE010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 4854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4854
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17732_length_4854_cov_3.410502"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            169..1185
                     /locus_tag="PKN52_01275"
     CDS             169..1185
                     /locus_tag="PKN52_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNQ98597.1"
                     /translation="MYLRGGVYPIQVRFKRSGTADQPIVWASYPGEWAVMDGSDKPVV
                     ESKDRVWVQGIQHNEFRNFEVRYGPQEGIYVESSNDNIFSGIVTHHNHYSGILNVYSN
                     RNLYEYVVSHDNFDYANPYGRIGDDADGISISSGDGNELYRVIVFNNSDDGIDTWIST
                     NTVVDGAISFSNGNGAYGNGNGIKAGGRDATVNTIVRNSIAFHNRAHGFDDNSGRGVQ
                     FIHTTAFDNGGYAYVAGSYTTLRYSIAYGGRGAGLWDAKSIANTWDMGIANPGFISTD
                     PTDDNFLTPDQNSPVAGKLDGEQSDLGAIQVGDKFGAWIAPVASYVNLASSAAVMTFA
                     TAGR"
     gene            1421..2890
                     /locus_tag="PKN52_01280"
     CDS             1421..2890
                     /locus_tag="PKN52_01280"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HNQ98598.1"
                     /translation="MRAVLNNLVRLGGMQFVLALTAVVRNKALAYRLGAEGFGEFSQL
                     AVLVIFGSTFVALGLGMSLNRNTAATPDAGLRQRFLAQANAVNLIGSVLVLSIGLAIA
                     LFRPEFLGAAGLPAVEGIRLSFAIMLAFIPLEVAMQHRIGFLTGMVDVQGLSASRSMA
                     LLIGTAASLPLIWFFGLVGAAAQFVLLSALNVLLLDRRCRFLGIRPWRVVWDRGVLKI
                     LAGLGVASVAAGMLHQLSDTLNRIFLMRWTDATQNGLYQAAVSITYQVKAVVLGSVGS
                     YAVATLSQDASSTRMSEVANQLLRLVLPIAAIALGMLGLFSGLAILVLYSPDFLGAQQ
                     ILPMLLAAEFLNVIAWVCGAPLLAEKRVAAWLFLEVLYATTKLAVSLPLIAGTGATGL
                     AAGYLIATAAHAFWTALFYRRSVGLTIEPANSLLMLLGIATVVFLGRLGENAVVDWAR
                     WSIGAATVTVFLLVAVRATMTYSEAVRLVHTMFTKIRKR"
     gene            2925..3764
                     /locus_tag="PKN52_01285"
     CDS             2925..3764
                     /locus_tag="PKN52_01285"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNQ98599.1"
                     /translation="MVTPVYNASSYVERAVQSVLTQKGIDSVEVILVNDGSTDGSDVV
                     IDELAARSTQVVAIHSENRGPSAARNLGIARATGRYLAFLDADDWFLPHKLAFQTGIL
                     ETRSKLGIVTGDHRLLNEDTGFTLDVVRGKPAIPYSEALAYRNWFAPMAPLLRMSVAE
                     AVGGFDASLRASEDWDYWYRCAQLAEIHYEPGVVGVYRLHGEQSHRNRAKMIASHLRF
                     IEKHLSDKPRLRRGALAYFHREEAKLAKGRGDWPAVSTHLAKMCYFAGSAARVAFIWN
                     LPR"
     gene            3761..>4854
                     /locus_tag="PKN52_01290"
     CDS             3761..>4854
                     /locus_tag="PKN52_01290"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNQ98600.1"
                     /translation="MTTLPSHPSTPKWAIALLLVLAVTTGALVVVSPVAAVSAQLLLI
                     GGIVLVRYEHLSEFLVGAYWITFCAYETIFKDVQIDGLFYPFYALMLVGILLRVGFAE
                     IRKPRTTIFWYVAFLLVVLLSFIGFTDPITFAVIQRILAYVFGLLIVLQVASRRGVTV
                     VAHMAILSSTILSVWVIVVAVQGGFAYRGGVEGDQNVIAMFIGLGAVIAFGRSVHGLV
                     TRNTGATALSLLLSAVSIYALLLLASRGITIAFAITAGAMFLRLVVQAPKTVLPTLLL
                     VAALSGASFLLPGGSSLVQRFSGERVESGGSRLPLWEATWSAYTESDATQLLIGHGFD
                     SSKALIRQVSYNLTSTHNAYFQVLYEFGLAG"
BASE COUNT          860 a         1621 c         1487 g          886 t
ORIGIN      
        1 ccgcccacca cgccgccggt agcaccgagc cttcccggca cggagcgccc gctccccgcg
       61 gcaacgatgt acgtatcgcc caacggtagc gcgtcgacca acggtcgctc cattgacgca
      121 ccgacgaccc tggcacacgc aaccgaactg gtcgagccgg gagacgtcgt gtacctaagg
      181 ggtggcgtct accccatcca ggtgaggttc aagcgctccg gaaccgccga ccagcctatc
      241 gtatgggcct cctaccctgg cgaatgggcc gtcatggacg gttcggacaa gccggtggtc
      301 gagagcaagg accgggtgtg ggtccaaggg atccaacaca acgagttccg caacttcgag
      361 gtgcgctacg gcccccaaga aggcatctac gtcgagtcct ccaacgacaa catcttcagc
      421 ggcatagtca cgcatcacaa ccactacagc ggcatcctca acgtgtacag caaccggaac
      481 ctctacgagt acgtggtcag ccatgacaac ttcgactacg ccaaccctta tggtcggata
      541 ggcgacgatg ccgacggcat cagcatctcc agcggcgacg gcaacgagct ttaccgtgtg
      601 atcgtcttca acaactcgga tgacggcatc gacacatgga tctcgacgaa cacggtggtc
      661 gacggggcca tctcgttcag caacggcaac ggggcgtacg gcaacggcaa cggcatcaag
      721 gccgggggtc gagacgccac ggtgaacacg atcgtccgca actccatcgc gttccacaac
      781 agggcgcacg gcttcgacga caacagtggc aggggcgtgc agttcatcca caccacggcg
      841 ttcgacaacg gcggctacgc gtacgtcgcc ggcagctaca ccacccttcg ctacagcatc
      901 gcctacggtg ggaggggcgc cggcctctgg gacgccaaga gcatagccaa cacgtgggac
      961 atgggcatag cgaacccagg cttcatcagc acggacccca ccgacgacaa cttcctaacc
     1021 ccggaccaga actcgcccgt ggccggcaag ctggacggtg agcagagcga tctcggcgcc
     1081 atccaggtcg gcgacaagtt cggtgcgtgg atcgctccag tggccagcta cgtcaacctg
     1141 gcgagctcgg ccgccgtgat gacgttcgcg acggccggac ggtagagaac ggccggacgt
     1201 ttgagcgtgc cggccatcaa cgccagtcac actcgctagc gtctcggcac tcccgaatga
     1261 ccctgtagcg cgacaccgag gcctcaagtc cgccggccgg gcggcagccc acagaagcgt
     1321 agaatctgtg ggctgccgcc cggccgacct ggtctagacg ccattggcta tgggatgacc
     1381 acatcggatg gccagctgta gctagggaag agggccggct atgcgagcag ttctgaacaa
     1441 cctggtcagg ctgggtggca tgcagttcgt tctcgccctg acggccgtcg tgcggaacaa
     1501 ggcgttggcg taccgccttg gagcggaagg cttcggggag ttcagccaac tcgccgtcct
     1561 cgtcatcttc ggcagcacct tcgtagctct cggcctcggc atgagcctca accgcaacac
     1621 cgccgcgacg ccggacgccg ggctgaggca gaggttcctg gcccaagcga acgccgtcaa
     1681 cttgatcggc agcgtgctgg tgctctcaat cggactcgcc atcgcactct tccgcccgga
     1741 gttcctggga gccgccgggc ttcccgccgt cgagggcatc cgactgtcct tcgcgatcat
     1801 gctggccttc atacccctgg aggtggccat gcagcaccgc atcgggttcc tgacggggat
     1861 ggtcgacgtg cagggcctct cggcgagccg tagcatggcg ctgctaatag gtaccgctgc
     1921 ctcgctgccg ctgatctggt tcttcggcct cgttggtgcg gccgcgcagt tcgttctgct
     1981 ctccgcactc aacgtgctgc tcctcgacag gcgctgtcgc ttcctaggga tcaggccttg
     2041 gcgcgtggtg tgggacaggg gcgtcctcaa gatcctggct ggcctcggcg tggcctcggt
     2101 tgccgccggg atgctccacc agctctcaga taccctgaac cgcatcttcc tcatgcggtg
     2161 gacagacgcg actcagaacg gcctctacca ggcggctgtc tccatcacat atcaggtaaa
     2221 ggccgtcgtg ctcggcagcg tcggcagtta cgccgtcgcc accctgagcc aggacgccag
     2281 ctctaccagg atgagtgagg tagccaacca gctcctgcga ctcgtgttac ccatcgccgc
     2341 catcgcgctc ggcatgctag ggctcttctc agggctcgcc atcctagtgc tctacagccc
     2401 tgacttcctc ggggcccagc agatcctgcc gatgctgctc gcggcagagt tcctcaacgt
     2461 catagcctgg gtatgcgggg ctccactctt ggccgagaaa cgggtagcgg cgtggctgtt
     2521 cctcgaggtc ctgtacgcga cgacgaagct cgcggtctca ctacccctga tcgccggtac
     2581 cggggccacc gggctcgccg ccggctacct catagcgacg gcggcccacg ctttctggac
     2641 tgccctcttc taccgacgct ccgtcgggtt gacaatcgag cccgccaaca gcctcctcat
     2701 gctgctgggg atcgcgactg tggtctttct ggggcggctc ggcgagaacg cggtcgtaga
     2761 ctgggcgagg tggtccatcg gcgcagccac ggtaaccgtc ttcctgctgg tcgcggtgcg
     2821 agccaccatg acctactcgg aagccgtgcg tctcgtgcac acgatgttca ccaagatccg
     2881 caagagatga gaggcggcgg tcgacgttgc agccgacggt aagcgtggtc acccctgtct
     2941 ataacgcctc ctcctacgta gagcgagcgg tgcagagcgt cctaacgcag aaaggcatcg
     3001 actctgtcga ggtgatcctg gtcaacgacg gctccacgga cggatcggac gtggtcatcg
     3061 acgagcttgc ggccaggagc acgcaggtcg ttgcgatcca ttcggagaac cggggaccta
     3121 gcgccgcgag gaacctcggc atcgcgaggg ccaccgggcg ctacctggct ttcctggacg
     3181 ccgacgactg gttcttacct cacaagctcg cttttcagac cgggatactc gagacgcgct
     3241 ccaaactagg catcgtcacg ggcgaccacc ggctactgaa cgaggacacg ggcttcacgc
     3301 ttgacgtagt gcgaggcaag ccggccatcc cctactccga ggccttggcg tacaggaact
     3361 ggttcgcgcc gatggccccg ctactgcgca tgagcgtggc tgaagcggtc ggggggttcg
     3421 acgcaagcct gcgggcctcg gaagactggg actactggta ccgctgcgcc cagctggcag
     3481 agatccacta cgagcccggc gtggtgggcg tctacaggct acacggcgag cagtcccacc
     3541 ggaaccgcgc caagatgatc gcgtcccacc tgcggttcat agagaagcat ctgagcgata
     3601 agccacggtt gcgtaggggt gccctcgcct acttccacag ggaagaggcg aagctggcga
     3661 agggccgtgg cgactggccg gccgtgtcga ctcacttggc gaagatgtgc tacttcgcgg
     3721 gttcggccgc cagggtagct ttcatatgga acctaccgcg atgacgacgc tccctagcca
     3781 cccctcgaca ccgaagtggg ccatcgccct cctgctggta ctcgccgtaa ccacgggggc
     3841 gctcgtcgtg gtctccccgg tcgccgccgt ctccgctcag ctgctcctca tcgggggcat
     3901 cgtgttggtg cgctacgagc atttgagcga gttcctcgtc ggcgcttact ggatcacgtt
     3961 ctgcgcgtac gagaccatct tcaaggatgt ccagatcgac ggtttgttct accccttcta
     4021 cgctctgatg ctcgtcggca tcctcctgcg cgtcgggttc gccgagatca ggaagccacg
     4081 gaccacgatc ttctggtacg tggcgttcct acttgttgtc ctgctctcgt tcatcggctt
     4141 caccgatccg atcacgttcg ccgtgatcca aaggatcctt gcttacgtgt tcggtctgct
     4201 gatagtgctc caggtcgcct ccaggcgggg cgtgacagtc gtcgcgcaca tggccatcct
     4261 gtcaagcacg atcctctcgg tgtgggtgat cgtcgtggcc gtccaaggcg ggttcgctta
     4321 ccgcggaggc gttgagggcg accagaacgt catagccatg ttcatagggc tgggggcagt
     4381 gatcgcgttc ggtagaagcg tgcacggcct cgtgacccgc aacaccggcg cgacagcgct
     4441 atccctcctc ttgtccgccg tctccatcta cgccctcctc ctcctcgcct cgcgcggcat
     4501 cacgatcgcc ttcgccataa cggccggagc catgttctta agactcgtcg tgcaggcgcc
     4561 gaagacggtc ttgcccaccc tgctcctggt ggctgcgctc tccggggcct ccttcctcct
     4621 ccctggcggc tcgagtctgg tccaacgctt cagcggtgag cgcgtcgaga gcgggggtag
     4681 ccgcctgcca ctatgggaag ccacctggag cgcctacacc gagagcgacg cgacccaact
     4741 gctcatcggt cacggcttcg acagcagcaa ggcgctcata aggcaggtct cgtataatct
     4801 gacctccacg cacaacgcct acttccaggt cctgtacgag ttcggcctgg ctgg
//
LOCUS       DAOQYE010000037         4851 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_17752_length_4851_cov_2.503753, whole genome shotgun sequence.
ACCESSION   DAOQYE010000037 DAOQYE010000000
VERSION     DAOQYE010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 4851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4851
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17752_length_4851_cov_2.503753"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="PKN52_01295"
     CDS             <1..352
                     /locus_tag="PKN52_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98601.1"
                     /translation="VLGRELTAGWRLARGEAIGDVAGAVPAGAPVAIVLGSPTRGIDV
                     RGMRYLHALIRRYRDAGCAVLLVSTDLDEALALSDRIVVLAGGAVAATLPGAGATTKE
                     ELGRYMLGAVSTPS"
     gene            349..1446
                     /locus_tag="PKN52_01300"
     CDS             349..1446
                     /locus_tag="PKN52_01300"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ98602.1"
                     /translation="MSPTALNRVADAMGLVATLVVSLGVGTLLVVATSAEPGVALRAF
                     FAGPFTNAFFFGNMLQAAAPLIITGLGAVLAFRAGAVNLGVEGQVYAGGLAGGAALLL
                     AGVHSGWFLPVAVLVGALAGGALAALAGWLRVRFGASEAISSFLIGAAVIQLFDLALR
                     RYLVDPAAAFPATRAIEPALRLARLAPPSNLNLGFFVGVALAALVFLLLFRTRFGYGL
                     RLTGSNPRFARYSGLRTRRLVVWAMLLSGAFAGAAAVLEVAGVHGRLITGFSNDLGWN
                     GITVALIARLHPLGVIPAALLYGYLQVDASSASLLSDVSPRVATVVQSVIFYLITAQA
                     LYVWFRRRLVARGAVPGERSIAQRPGTGSGA"
     gene            1443..2360
                     /locus_tag="PKN52_01305"
     CDS             1443..2360
                     /locus_tag="PKN52_01305"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ98603.1"
                     /translation="MSLVGVFDLQYLHDVVRLSAPIAVVALAGALTQRAGLMNIGLEG
                     LLLFGAFAAVLGAGLTGSLAIGTLAAALAGALLALAFGWFTLRLRANVFIVGLATNAF
                     SAAATAYAAWLLSGQEGQLRLEGMPTLPRLELPLLARFEPLSFLSGHTALDYLAWLLV
                     PIVAYVLFRSRFGLRLRATGEDPAAARAAGLRVDALRYAAIAASGLLAGLAGAQLSLS
                     LGGFVQDMSGGRGWVGLVAAIVGGATAWGSAGVALLFGAAQGAANSLQLSARGVAPQL
                     LLAFPYVLTLVALVGYSAVRSRRRGASEE"
     gene            complement(2434..3342)
                     /locus_tag="PKN52_01310"
     CDS             complement(2434..3342)
                     /locus_tag="PKN52_01310"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNQ98604.1"
                     /translation="MRFVCMTPNAAIDRILHLNQPRALHGLNRVARAIESAGGKGMNV
                     ARVLRGLGAEVSVAGFLAGLNAARFRALLDRDGLAGHFVTVTGETRECQILVDGRGEP
                     MEINDPGPLAAADDWERLAAELPEGRLVISGSLPPGLSPEEFGEFIVALGERRGEAPV
                     VDTSGPYLVAAVAAGAALVKPNEHEIGLLSGTPDPVEAALETYARTGVPVLLTLGAVG
                     AAYVGGESVFVPAPVIDAVNPVASGDCLLAAFLWASASGWPAGDALRLGVAAGAANAK
                     SGGGGRVGGAQVFEQFAQVEEARALG"
     gene            complement(3347..4357)
                     /locus_tag="PKN52_01315"
     CDS             complement(3347..4357)
                     /locus_tag="PKN52_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98605.1"
                     /translation="MPRRHPARNARGRTALVAVLLVGVLCGCEGSRSQTPPPGRGPDL
                     AGCPMLPADNVWNARVDTLPVLTESRAYVASIGASSSVRADFGSGLWEGAPIGIPYTT
                     VEAGQPAVSVSFYYPDESDRGPYPIPRDAPVEGAPPGGVAPGGDRHVLVVDLSGCTLY
                     ELYDAELQRDGSWEAGSGAIYDLASNDLRPDGWTSADAAGLPILPGLVRYEEVEAGEI
                     AHAIRFTAPRTRNDYVWPARHQASSRSDPELPPMGQRFRLRADFDASGMAPEVQVIVT
                     AMKRYGLVLADNGSSWFLSGAPDERWDNDMLRQLMQINGSDLEAVSVLGLMTDPDSGR
                     VR"
     gene            4564..>4851
                     /locus_tag="PKN52_01320"
     CDS             4564..>4851
                     /locus_tag="PKN52_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98606.1"
                     /translation="MEGLVDLFAKLIGPHPVWQGFWGGMVITGFNLIGALAVLVWRNP
                     TRRSLDGALGFAAGVMLTASFTSLILPGIEIGGLLPVVIGVILGVVMLDRAD"
BASE COUNT          572 a         1762 c         1741 g          776 t
ORIGIN      
        1 cgtgctcggg cgggaactga ccgccggctg gcgtttggcg cggggagagg cgatcggcga
       61 cgtagcgggg gcggtcccgg cgggcgctcc cgtcgccatc gtcctcggct cgccaacgcg
      121 cggcatcgac gtgcgcggta tgcgttacct ccacgctctc atccgccgct accgcgacgc
      181 cggctgcgcc gtgctgctcg tctccaccga cctcgacgag gcgctggcgc tcagcgaccg
      241 catcgtcgtc ctggccggcg gcgctgtggc ggcgacgttg cctggcgcag gcgcgacgac
      301 caaggaggag ctgggacgct acatgctcgg ggcggtgagc accccttcgt gagcccgacc
      361 gccctcaacc gcgtcgctga cgccatggga ctggtcgcca ccctggtcgt gtctctcggc
      421 gtcggcacgc tcctcgtggt ggccacgagc gccgagccgg gcgtcgcgct gcgggcgttc
      481 ttcgccgggc cgttcacgaa cgccttcttc ttcggcaaca tgttgcaggc cgccgcgcca
      541 ctgatcatca ctggcctcgg cgccgtgctc gctttcaggg ccggcgcggt gaacctgggc
      601 gtcgagggtc aggtgtacgc gggcgggctc gccggcggcg cggcgctgct cctggccggc
      661 gtgcatagcg gctggttcct ccccgtggcg gtgctcgtcg gggcgctcgc cggtggggcg
      721 ctcgcggccc tcgccggctg gctgcgcgtc cggttcgggg ccagcgaggc catctcgtcg
      781 ttcctgatcg gcgcggccgt catccagctc ttcgacttgg cgctcaggcg ttacctcgtc
      841 gacccggcgg cggcgttccc cgccacccgc gccatcgagc cggcgctgag gctggcgcgg
      901 ctcgcacccc cgtcgaacct caacctcggc ttcttcgtcg gcgtcgccct ggcggcgctc
      961 gtcttcctcc tgctcttccg cacgcgcttc ggctacggtc tgcgcctgac ggggagcaac
     1021 ccgcgcttcg cgcgctacag cggtctgcgc acgcggcgcc tggtcgtatg ggccatgctc
     1081 ctgagcggcg ccttcgcggg cgccgcggcc gtgctggagg tggccggcgt gcacggacgc
     1141 ctcatcacgg gcttctccaa cgacctcggc tggaacggca tcacggtggc gctgatcgcg
     1201 cgtctgcacc ctcttggcgt catccccgcc gccctcctgt acggctacct gcaggtcgac
     1261 gccagttccg cgagcctcct gtccgacgtc tcaccgcgcg tcgctaccgt cgtccaatcc
     1321 gtgatcttct acctgatcac agcccaggcg ctctacgtat ggttccgccg gcggttggtc
     1381 gcccggggcg ccgtaccggg cgagcggagc atcgctcaga ggccgggaac cgggtctggc
     1441 gcatgagctt ggtcggcgtg ttcgacctgc agtacctgca cgacgtcgtt aggctcagcg
     1501 ctcccatcgc cgtggtggcg ctggcgggag cgctcacgca gcgcgcgggg ctcatgaaca
     1561 tcggtctgga agggctgctc ctcttcggcg ccttcgcggc ggtgctgggt gccggcctga
     1621 ccggcagcct ggcgatcggc accctggcgg cggcactggc cggcgctctg ctggcgctcg
     1681 cgttcggctg gttcacgctc cgcctgcgcg ccaacgtctt catcgtcgga ctggccacga
     1741 acgcgttctc cgccgccgcc acggcctacg cggcatggct gctctccggg caggaggggc
     1801 agctgaggct cgaaggcatg ccgacgctgc cacgcctcga actgccgctg ctcgcgcgct
     1861 tcgagccgct gtccttcctg agcggtcata cggcgctcga ctacttggcc tggttgctcg
     1921 tgccgatcgt ggcgtacgtg ctgttccggt cccgcttcgg cctacggctc cgcgcgacgg
     1981 gcgaggaccc cgccgcggct cgggctgcgg gcctgcgggt ggacgcgctc aggtacgcgg
     2041 ccatcgctgc cagcggtctg ctcgccggtc ttgccggtgc ccagctgtcg ttgtcgttgg
     2101 gtgggttcgt gcaggacatg tcgggtggcc ggggctgggt tggcctcgtg gcggccatcg
     2161 tcggcggcgc gacggcctgg ggctcggcgg gcgtcgcgct cctcttcggc gccgcgcagg
     2221 gcgcggcgaa ctcgctgcag ctcagcgcgc gaggcgttgc gccccaactc ctccttgcct
     2281 tcccatacgt gctgaccctg gtcgcgctcg tcggatacag cgcggtgcgc agtcggaggc
     2341 gcggcgcgtc agaggagtga gggcggtcgt ggctttcctg gcgcggcgac ccggccctgg
     2401 gcaagggtcg gtcatccggg accgaaccgc gcctcaaccg agcgcacgcg cctcctcgac
     2461 ctgcgcgaac tgctcgaaca cctgcgctcc tccgacccgc ccgccgccgc ccgacttggc
     2521 gttggccgcg cccgcggcca cgcccagccg cagcgcgtcg ccggccggcc acccggaggc
     2581 gctcgcccag aggaaggcgg ccaggaggca gtcgccggag gcgaccgggt tgacggcgtc
     2641 gatgaccggc gcgggtacga agacgctctc cccgccgacg tacgccgccc cgacggcgcc
     2701 cagcgtgagc aggaccggca cgccggtgcg cgcgtaggtc tcgagcgctg cctcgacggg
     2761 gtcgggggtc ccggacagca gcccgatctc gtgctcgttc ggtttgacga gggcggcgcc
     2821 ggcagccacg gccgctacga gatacgggcc gctcgtgtcg acgaccggcg cctcaccgcg
     2881 gcgctcgccg agcgccacga tgaactcgcc gaactcctcc ggggagaggc cgggcggcag
     2941 gctgccagag atcaccaggc gcccttccgg cagctccgcg gcgagtcgct cccagtcgtc
     3001 ggccgcggcc aacggccccg ggtcgttgat ctccatcggc tcgccgcgtc cgtccaccag
     3061 gatctggcac tcgcgcgtct caccggtcac cgtgacgaag tgccctgcca ggccgtcgcg
     3121 atcgaggagc gccctgaacc tcgcggcgtt caggcctgcg aggaagccgg cgacggagac
     3181 ctcggcgccg agacctctca gtacgcgtgc tacgttcatc cccttgccgc ctgccgactc
     3241 gatcgcgcgc gccacgcggt tgaggccgtg cagggcgcgc ggctggttga ggtgcaggat
     3301 gcgatcgatg gcggcgttcg gcgtcatgca gacgaaacgc acgcgctcac ctgacccggc
     3361 cggagtcggg gtcggtcatg aggccgagga cgctcacggc ttccaggtcg ctcccattga
     3421 tctgcatgag ttgccgcagc atgtcgttgt cccagcgctc gtccggcgcg cccgacagga
     3481 accagctgga gccgttgtcg gcaaggacga ggccgtaacg cttcatggcc gtgacgatga
     3541 cttgcacctc cggcgccatg ccgctcgcgt cgaaatcggc gcgcaggcgg aagcgctggc
     3601 ccatgggcgg cagctccggg tcgctccggc tcgaggcctg atggcgagcc ggccacacgt
     3661 agtcgttgcg cgtgcgtggc gcggtgaaac ggatggcatg ggcgatctcg ccggcctcca
     3721 cctcctcgta gcgcacgagg ccggggagta tcgggaggcc ggcggcgtcg gccgacgtcc
     3781 agccgtccgg cctgaggtcg ttgctcgcca agtcgtagat ggcgcccgag cccgcctccc
     3841 agctcccgtc gcgctgcaat tcagcgtcgt acagctcgta gagcgtgcag ccggagaggt
     3901 cgaccacgag gacgtggcgg tcgccgccgg gtgctacccc gcctggaggc gcaccctcca
     3961 cgggggcgtc acgggggatg gggtacggcc ctcgatcgga ctcgtccggg tagtagaagc
     4021 taacgctcac ggcgggctgt cccgcttcga ccgtcgtgta cgggatgccg atcggagcgc
     4081 cctcccacag gcccgagccg aagtcggcgc gcaccgacga ggaggcgccg atcgacgcga
     4141 cgtaggcgcg cgactcggtc agcacgggca gtgtgtcgac gcgggcgttc cagacgttgt
     4201 cggccggcaa catggggcag ccggcgaggt ccggcccgcg ccctggtggc ggggtctgcg
     4261 acctgctccc ctcgcagccg cagagcaccc ccaccagcag cacggcgacg agcgcggttc
     4321 ttcccctggc gttgcgggcc ggatggcgtc gtggcatgcc ccatcatggt ccggtgccga
     4381 tgtggggagt gtgggaagtc tcccggccgg cccgcgcccc gtggggaaac agcaggtgga
     4441 ggcgagcaag gcgttcgcgg ccgcggccgg tccgcgccct gtggggaacc tcggcctggt
     4501 gcggccgcgc gcccgctgcc gaccaggcca ctcccacggt ggggagaaca taaactggcg
     4561 ccgatggaag gcctcgtcga cctcttcgcg aagcttatcg gcccgcaccc cgtctggcag
     4621 ggcttctggg gcgggatggt cataaccggc ttcaacctga tcggcgccct ggccgtcctg
     4681 gtctggcgca atcccacgcg acgctcactc gacggcgcgc tcggcttcgc ggccggggtg
     4741 atgctcaccg cgagcttcac cagcctcatc ctccccggta tcgagatcgg tggcctgctc
     4801 ccggtggtca tcggcgtgat cctcggcgtc gtcatgctcg accgcgcgga c
//
LOCUS       DAOQYE010000038         4680 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_18637_length_4680_cov_2.210162, whole genome shotgun sequence.
ACCESSION   DAOQYE010000038 DAOQYE010000000
VERSION     DAOQYE010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 4680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4680
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18637_length_4680_cov_2.210162"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(138..3017)
                     /locus_tag="PKN52_01325"
     CDS             complement(138..3017)
                     /locus_tag="PKN52_01325"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HNQ98607.1"
                     /translation="MSPSERRFTAFVIPHTHWDREWYQPFKVFQARLVDVIDSALDLL
                     DDPAYRRFTLDGQAAVLEDYLALRPEREGDLRRHVQAGRLRIGPWYVLADEFLVSPES
                     LIRNLLVGVRVSRRFGDPLPVCYTPDSFGHISQLPLFADGFGLPAIVFERGLGDEGER
                     LRGEFVWVAADGKTEVFTAHLIGTYSGATALGHVDWELTDAYDDDRAMGHLRAALYGV
                     QGDEVADLPEWFRASLERVHGGLTAHATGSALILLNGSDHLFPQPNLPEVVRRASQAF
                     PEIEFVQGDVEEFVTEARRTAAGLERFQGEFRGSRYQHILAGVLSARLYLKRENQACQ
                     TALEEYAEPLSALAWWASGRHQDALLREAWRLLLLNHPHDSICGCSVDAVHREMLTRF
                     ENVRQLGDDVCRRAFALLGGRRPSNIQEVHATAKDAITVFDPLPFPRRAVVRHELDLP
                     AGEAAGLAVLDASGRALNAQVTVTPGFAPGQSSVRVDHVAIDVLADLEPLGLTNLFLA
                     RAVSGSGSSPGELEQPAARLAADAAANPHASTDTSDTAARAASLVAATAGHDGVRLEN
                     GHVTVSVSSGGAVTLFERATGERYALNLRFEDQGDAGDEYDFSPVPGDTPAYFSAPCR
                     PPRLLTSGPVVAGARLEYALTLPEHLSDDRTRRAGSVSLPITLDLTLHADDPLLRLSA
                     SFDNTASDHRLRLRLDTGAITETVWADGHFDTLERPVRPALGADWFQVPPPTNHQRRF
                     VAVSGAGRGLAVLNRGLPEYEAFHGEGGTELAVTLVRSVGWLSRSDLLSRPQGAGPLL
                     PTPEAQCLGAHRCELAIYPFAGPWWDTDLLRHAQAFAAPPLTAPGSSSGAGPGLLRVD
                     GPFDLSAVKRSEERESLIVRVANPSPVPASGRLRFGRPVRAVYATRLDETRLDETRLD
                     EARVVEARLADLDAASDLPLQLGPRSVASFEFVPGEGVMPSIERG"
     gene            complement(3023..4015)
                     /locus_tag="PKN52_01330"
     CDS             complement(3023..4015)
                     /locus_tag="PKN52_01330"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNQ98608.1"
                     /translation="MSTIRDIAREAGVSVSTASLALNGDARVRPDTRLRVLTAAEQLD
                     YHPSRTAKNLSSGRTWSLHLMNPMRDAGLSSTFFTRFVRGVHDVVAALDYTLALTVLD
                     DEAEADEILRKLVLERWTDGVILMNVSEEQSFLQKLLQHEFPHVLLGHSTLPGVTSVD
                     NDNHAVAADATAHLLARGRQPLAFLNGPAPHAFVQERAKGFRLAHERAGLRAAEWQVQ
                     FGVTSAEGARTRVRQLLESGERFGGVLANSDEAAIGALRAVRDAGLHVPNDVAIVGIN
                     NDDLTEYTDPRLTSVELNAAELGRVAAELLIRSIDRHPAERRLVPHHLVQRDSS"
     gene            4364..>4680
                     /locus_tag="PKN52_01335"
     CDS             4364..>4680
                     /locus_tag="PKN52_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98609.1"
                     /translation="MEEGRGGDVGTPPRGRGEGRSGVPEAVRSEIRVANAPCSWGLIG
                     QGDPGTGYARMLDELVETGYVGTELGDYGYMPTDPAALRDELTGRGLTLLGAFAGVTL
                     RERG"
BASE COUNT          678 a         1519 c         1809 g          674 t
ORIGIN      
        1 gagcgactgc gagaacgccg gcgacattat ggcgcccagg acgagcaggg tgaacagcgc
       61 gatgatgcgt ttcttcatgc gtttcctccg atgaggttgg ggtgtgcgaa gggcggttgc
      121 cctcctcaga gccgtcgcta gccacgttcg atgcttggca tcaccccctc tcccggaacg
      181 aactcgaagc tggccacgct gcgtggccca agctgcaggg ggaggtccga ggcggcgtca
      241 aggtccgcca ggcgcgcctc gacgacgcgc gcctcatcga gacgtgtctc atcgagacgt
      301 gtctcatcga gacgcgtcgc gtagaccgcg cgcacggggc gcccgaagcg cagccgaccg
      361 ctggcgggga ccggactggg gttcgcgacg cggacgatga ggctctcgcg ctcctcggag
      421 cgcttgacgg ccgagaggtc gaaggggccg tcgacgcgca agagccccgg cccggcgccg
      481 ctggaggagc cgggggcggt gagcggcggt gcggcgaagg cctgcgcgtg ccgcaggagg
      541 tcggtatccc accatggtcc cgcgaagggg tagatggcca gctcgcagcg gtgcgcgcca
      601 aggcactgcg cttccggcgt cggcaggagc ggcccggcgc cttgggggcg gctgagcagg
      661 tcggagcgcg acagccagcc cacggagcgc acgagcgtga cggccagctc cgtgccgccc
      721 tcgccgtgga acgcctcgta ctccggcagt ccgcggttga ggaccgccag gccgcgcccg
      781 gcgccagaca cggccacgaa gcggcgctgg tggttggtcg gcggcggcac ctggaaccag
      841 tccgcgccta aggcgggtcg caccggccgc tcgagggtgt cgaagtgacc gtcggcccac
      901 accgtctcgg tgatggcgcc ggtatccagg cgcagccgga ggcgatggtc ggaagccgtg
      961 ttgtcgaagc tggcgctcag gcgtagcagc gggtcgtcgg catggagggt caggtcgagc
     1021 gtgatgggca gggacacgct cccggcgcgg cgcgtgcgat cgtcgctcag gtgctcgggc
     1081 agggtgaggg cgtactctag gcgcgcgccg gccacgaccg ggccgctggt cagtaggcgc
     1141 ggtggccggc acggggcgga gaagtaggcg ggcgtgtcgc ccgggacggg cgagaagtcg
     1201 tactcgtcgc ccgcgtcgcc ctggtcctcg aagcgcaggt tcagcgcgta gcgctcgccc
     1261 gtggcgcgct caaacagcgt tacggcgccg ccgctgctca cggacacggt cacgtgaccg
     1321 ttctccaggc ggacgccgtc gtggccggcc gtggcggcga ccaacgaggc ggccctcgcc
     1381 gctgtgtcac tcgtgtcggt ggaggcatgg gggttggccg ccgcgtcggc ggcgagccgc
     1441 gccgcaggct gctcgagctc gccggggctc gaccccgacc cggacactgc gcgcgccagg
     1501 aagaggttgg tgagcccgag cggttcgagg tcggccaaca cgtcgatggc cacgtgatcg
     1561 accctcacgc tgctctgccc gggcgcgaac cctggcgtga cggtgacctg ggcgttcagg
     1621 gcgcggccgc tcgcgtccag cacggcgagt ccggcggctt cgccggcggg caggtcgagt
     1681 tcgtgccgca ccaccgcgcg cctggggaag gggagggggt cgaagacggt gatggcgtcc
     1741 ttcgccgtcg cgtggacctc ttggatgttg ctcggcctcc ggccgccgag gagcgcgaac
     1801 gcccggcggc agacgtcgtc gccgagctgg cgcacgttct cgaagcgcgt gagcatctcg
     1861 cggtggaccg cgtcgacgga gcagccgcag atggagtcgt gcgggtggtt caggagcagg
     1921 agccgccacg cctcgcgcag cagggcgtcc tggtggcgac cgctcgccca ccacgccagc
     1981 gcggagagcg gttcggcgta ctcctcgagc gcggtctgac acgcctggtt ctcgcgcttc
     2041 aggtagaggc gggccgagag gacgccggcc aggatgtgct ggtagcggct gccgcggaac
     2101 tcgccctgga accgctccaa cccggcggcg gtgcggcggg cctccgtcac gaactcctcc
     2161 acatcgccct gcacgaactc gatctcgggg aacgcctggg aggcccgccg gaccacctcg
     2221 ggcaggttcg gttgcgggaa caggtgatcg gagccgttga ggaggatgag ggcgctgccg
     2281 gtcgcgtgcg ctgtcaggcc gccgtgcacg cgctcgagcg aggccctgaa ccattcgggc
     2341 aggtcggcga cctcgtcgcc ttgcacaccg tagagagcgg cacggaggtg gcccatggcg
     2401 cggtcatcgt cgtacgcgtc cgtcagctcc cagtcaacgt gaccgagcgc cgtggcgccc
     2461 gagtacgtgc cgatgaggtg ggcggtgaag acctccgtct tgccgtctgc cgcgacccag
     2521 acgaactcgc cacggagccg ctcgccctcg tcgccgaggc cgcgctcgaa gacgatggcc
     2581 ggaaggccga agccgtctgc gaagaggggg agctgggaga tgtgcccgaa cgagtccggc
     2641 gtgtagcaga cgggcagcgg gtcgccgaag cgccggctca cccggacccc gaccagcagg
     2701 ttgcgtatca acgactcagg gctcacgagg aactcgtccg ccagcacgta ccagggtccg
     2761 atgcgcaggc gcccggcctg gacgtggcgg cggaggtcgc cctcgcgctc gggccgcagg
     2821 gcgaggtagt cctcgagcac cgccgcctga ccgtcgagcg tgaagcggcg gtaggcgggg
     2881 tcgtcgagga ggtcgagggc ggagtcgatg acgtccacga gccgcgcttg gaagaccttg
     2941 aacggttggt accactcgcg gtcccagtgt gtgtggggga tcacgaaggc ggtgaagcgg
     3001 cgctccgaag gggacatgat cgtcagctcg aatctctctg cacgaggtgg tgcggcacga
     3061 ggcggcgctc ggccgggtga cggtcgatgc tgcggatcag gagttcggcg gccacgcgcc
     3121 cgagctcggc ggcgttcagc tcgacgctgg tgaggcgcgg gtcggtgtac tccgtgaggt
     3181 cgtcgttgtt gatgccgacg atggcgacgt cgttgggcac gtgtagaccg gcgtcgcgca
     3241 cggcccggag cgcgccgatg gcggcctcgt cgctgttggc aagcacacca ccgaagcgct
     3301 cgccgctctc caggagctgc ctgacgcggg tccgcgcccc ctccgcagac gtgacgccga
     3361 actggacctg ccactcggcg gcgcgcagcc cggcccgctc gtgcgcgagc cggaagcctt
     3421 tggcgcgttc ctggacgaag gcgtgcgggg cgggtccgtt gaggaacgcg agcggctgtc
     3481 ggccccgcgc caggaggtgc gcggtggcgt cggccgccac cgcgtggttg tcgttgtcga
     3541 cgctcgtcac gcccggcagg gtactatggc cgaggaggac gtgcgggaac tcgtgctgga
     3601 ggagcttctg caggaacgac tgctcctcgg agacgttcat caggatgacg ccgtcggtcc
     3661 agcgctccag tacgagcttg cgcaagatct cgtccgcctc tgcctcgtcg tccagcaccg
     3721 tgagggcgag cgtgtagtcg agggcggcga ccacgtcgtg gacgccgcgc acgaagcgcg
     3781 tgaagaacgt ggaggagagc ccggcgtcgc gcatcgggtt catgaggtga agcgaccagg
     3841 tccggccgct cgagaggttc ttggcggtgc ggctcgggtg gtagtcgagc tgctcggccg
     3901 ccgtgaggac gcgcaggcgc gtgtcggggc gcacacgcgc gtcgccgttg agggccaggg
     3961 aggccgtcga cacggacacg ccggcctcgc gggcgatgtc gcggatcgtg ctcacgagcc
     4021 gacctcgcgt ttcgcccggc ggggctcttc gggcttcggt tccaagggca tgcggggttc
     4081 gttcacgagc ccattctcgt ccgcgccggc aaggcggcat gcgacggtgg cgcggggcag
     4141 taacatcaag ccgaagccgt tcatcgaacc ggttcggtga ggtgggttgc acgcgcacga
     4201 cgacgtcacg tcccttgacc tccactgctt gtcgaactgg ttcgatatta gggagcgacc
     4261 gctcgcctgt caaggacgag tgcacactgg taggaaacaa ggtgggtagg gctgggcggg
     4321 ttcgcgctac tcgacccgct cccgccgccc gttaggaggg ccgatggaag aaggtcgtgg
     4381 aggagatgtc gggacgccgc ctcgaggccg gggggaaggc cggtccggcg ttccggaggc
     4441 cgtccggtcg gagatcaggg tcgccaacgc gccctgctcg tgggggctga tcggtcaggg
     4501 cgaccccggc accggctacg cccgcatgct ggacgagctc gtcgagaccg gctacgtagg
     4561 cacggagctg ggagactacg gctacatgcc gaccgacccg gcggcgctcc gtgacgagtt
     4621 gaccggtcgc gggctcacgc tgctgggcgc cttcgccggc gtcaccctac gggaacgcgg
//
LOCUS       DAOQYE010000039         4646 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_18837_length_4646_cov_3.171205, whole genome shotgun sequence.
ACCESSION   DAOQYE010000039 DAOQYE010000000
VERSION     DAOQYE010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 4646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4646
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18837_length_4646_cov_3.171205"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="PKN52_01340"
     CDS             complement(<1..332)
                     /locus_tag="PKN52_01340"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNQ98610.1"
                     /translation="MNVTVRPARAEDVPLIFENIGYWASQGRMLVRPMQNIFENLRDF
                     FVAEVDDGSGPRFAGNGAMHILWGDIAEVRGLAVAPDVRARGVGRALVEACEHEARRV
                     GIPLLFAWT"
     gene            complement(329..1795)
                     /gene="argH"
                     /locus_tag="PKN52_01345"
     CDS             complement(329..1795)
                     /gene="argH"
                     /locus_tag="PKN52_01345"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HNQ98611.1"
                     /translation="MTDRDAKQREGAKASSSMWGGRFEEATDALVQRVNASIGVDQAM
                     AEDDLLGSVAHARMLVRQGILTEDEGNAMVGGLQELLAEVRAGKVEWREELEDVHMNL
                     ESVLTQRLGPVGGKLHTARSRNDQVTTDARLHLARRGAEVRDLVVELRRVIVSLAGQH
                     VDVIMPGYTHLQVAQPVRLSHHLLAYQEMFRRDQGRLEDALRRADVSPLGAGALAGTT
                     FPIDRAYTAELLGFGSVAANSMDAVSDRDYMLETLSALAILMMHLSRLSEELILWSSS
                     EFAFVALSDAYTTGSSIMPQKKNPDVCELVRGKTGRVYGALMGLLTVMKGLPLTYNKD
                     MQEDKEGLFDALSTASDCLRLYIGMLPGMRVNADRMRDAASRAYSNATDLADYLARRG
                     VPFREAHHVVGRLVATAIKRRVPLEALSLDDLKAEEPRIEADVYAALDLETVVDARRS
                     YGGTARERVLEQIAAAELELAAPSVGEPEAGAGGEDRR"
     gene            complement(1792..2229)
                     /locus_tag="PKN52_01350"
     CDS             complement(1792..2229)
                     /locus_tag="PKN52_01350"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNQ98612.1"
                     /translation="MPPRLREANASDAPALAALYAVARPQAQLSTAALREWLSRGRAL
                     LVEDALGRVVAALRHWEEGDGWRVEPIVTHPEHRGQGYGRWLMTMLEADAIRSNIPFL
                     ALTITDVSVLPYYQRLGYRPVGDDPKDLGKRVGGVWQRTAGQA"
     gene            complement(2287..3510)
                     /locus_tag="PKN52_01355"
     CDS             complement(2287..3510)
                     /locus_tag="PKN52_01355"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HNQ98613.1"
                     /translation="MSNERKPVKKVVLAYSGGLDTSVILHWIKAQYGAEVVAFTADIG
                     QGEEVAAAKAKALRTGAVAAYAVDLQEDFVRDFVFPVLRAGAIYEGYYLLGTSFARPL
                     IARKMVEIAEAEGADAVAHGATGKGNDQVRFELSAYALKPDVRVIAPWREWDLKGRED
                     CIAYAKAHGIDVPVTKEKPYSTDANLFHISYEGGVLEDPWAQPPAGMWKLTADPEAAP
                     DAPQVVTVSFEHGNPVAIDGERLGPVALLAKANEIAGRHGVGRVDIVENRFVGMKSRG
                     CYETPGGTLLFHAHKAVESLTLDRQVLQLRDDLVPRYAASVYNGFWFAPERVALQNLM
                     DDIQKPVTGEARLKLYKGGVTVLGRRSPNSLYRQDVVTFEEDSVYDQADADGFIKLNA
                     LRLRIAKQLESAKAK"
     gene            complement(3857..>4646)
                     /locus_tag="PKN52_01360"
     CDS             complement(3857..>4646)
                     /locus_tag="PKN52_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNQ98614.1"
                     /translation="VLARRIARREDLDDPVAVDIHGAALGEDDVGEGGAGGVHLDAGP
                     ERAVRLAIEAGGVSLAFILLYSAPAFVAVLAALLLGERLTPTKSALVAMSVVGVVLVA
                     QGGESGMTVSVRSVTWGLVAGASYSSYYLFGKWVLARYRPATIYAIVMPVGALGLAPL
                     TPFTALTNPTPRTWLLLFLMAAVSTYLAYFVYYTGLKRVEASRAVLVATIEPVVAAIL
                     AAWLFGERLGTLGIVGGALVVGAAMLSAVPARPARAARRGRPAA"
BASE COUNT          659 a         1594 c         1669 g          724 t
ORIGIN      
        1 gtccacgcga agaggagcgg gatgccgacg cgccgcgcct cgtgctcgca cgcctcgacg
       61 agcgcccggc cgacgccgcg agcgcgcacg tcgggcgcca cggccaggcc gcgcacctcg
      121 gcgatgtcgc cccacaggat gtgcatggcg ccgttgccgg cgaagcgcgg gccgctgccg
      181 tcgtcgacct cggcgacgaa gaagtcgcgg aggttctcga agatgttctg catcgggcgc
      241 acgagcatgc ggccctggct cgcccagtag ccgatgttct cgaagatcag ggggacgtcc
      301 tccgcccttg ccggccgcac cgtcacgttc atcggcggtc ctcaccgccg gcaccggctt
      361 ccggctcgcc aacgctcggc gcggcgagtt ccagctccgc tgccgcgatc tgctcgagga
      421 cgcgctcgcg cgccgtgccg ccgtaggagc gcctggcgtc gacgacggtc tcgaggtcca
      481 aggccgcgta gacgtcggcc tcgatgcgtg gctcctcggc cttcaggtcg tccaagctca
      541 gcgcctcgag aggcacgcgc cgcttgatgg ccgtcgccac gagccgaccg acgacgtggt
      601 gcgcctccct gaacggcacg ccgcgtcgcg ccaggtagtc ggcgaggtcg gtggcgttcg
      661 agtaggcgcg actggcggcg tcgcgcatgc ggtcggcgtt aacgcgcatg cccgggagca
      721 tgcctatgta gaggcgtagg cagtccgagg cggtcgacag cgcgtcgaac agcccctctt
      781 tgtcctcctg catgtccttg ttgtaggtga gcggcaggcc cttcatgacg gtgagcagcc
      841 ccatgagcgc gccgtagacg cggcccgtct tgccgcgcac cagctcgcag acgtccgggt
      901 tcttcttctg cggcatgatc gagctgcccg tcgtgtaggc gtcgctcagc gccacgaagg
      961 cgaactcgct cgacgaccag aggatgagct cctcgctcag gcgcgagagg tgcatcatca
     1021 ggatggcgag cgccgacagg gtctcgagca tgtagtcgcg atcggagacg gcgtccatgg
     1081 agttggccgc gacggacccg aagccgagga gctcggccgt gtacgcgcgg tcgatgggga
     1141 acgtggtgcc ggccagggcc cccgcgccga ggggcgagac gtcggcgcgc ctcagggcgt
     1201 cctcgaggcg cccctggtcg cgcctgaaca tctcctggta ggcgagcagg tggtgggaga
     1261 ggcgcacggg ctgggccacc tgcaggtgcg tgtagccggg catgatcacg tcgacgtgct
     1321 ggcccgccag gctcacgatg acgcggcgta gctccactac gaggtcgcgt acctcggcgc
     1381 cgcgccgcgc caggtgcagc cgggcgtcgg tagtcacctg gtcgttgcga ctgcgcgccg
     1441 tgtgcagctt gccgccgacc ggtccaaggc gctgcgtgag caccgactcg aggttcatat
     1501 gcacgtcctc gagttcctcg cgccactcga ccttgccggc gcgcacctcg gccagcaact
     1561 cttgcaagcc gccgaccatg gcgttcccct cgtcctccgt gaggatgccc tggcgcacga
     1621 gcatgcgcgc atgggcgacc gagccgagga ggtcgtcctc cgccatggcc tggtcgaccc
     1681 cgatggaggc gttgacccgt tgcacgagcg cgtcggtggc ctcttcgaag cggccgcccc
     1741 acatgctcga cgacgccttg gcgccttccc gctgcttggc gtcccggtcg gtcacgcctg
     1801 acccgctgtc ctctgccaca cgcccccgac gcgcttgccg aggtccttgg ggtcatcccc
     1861 gacgggcctg tacccgaggc gctggtagta ggggaggacg gaaacgtcag tgatggtcag
     1921 cgcgaggaac gggatgttgg agcggatggc gtccgcctcg agcatggtca tgagccagcg
     1981 gccgtagccc tggccgcggt gctccgggtg ggtgacgatc ggttcgacgc gccagccgtc
     2041 gccttcctcc cagtgcctca gcgcggccac gacccgaccg agcgcgtcct cgacgagtag
     2101 cgcacggccc ctggagagcc actcgcgcag cgcggccgtg ctcagctgcg cttgcggccg
     2161 cgcgacggcg tagagggccg cgagggcggg cgcgtccgag gcgttggcct cacgtagcct
     2221 gggcggcacg tagtctgtcc ccggttgctt cggtaggacg gcagcgcttg ccacgtcagt
     2281 aggccctcac ttcgccttgg cgctctcgag ctgcttcgcg atgcgcaggc gcagggcgtt
     2341 cagcttgatg aaaccgtcgg cgtcggcttg gtcgtagacg ctgtcctcct cgaacgtgac
     2401 gacgtcctgg cggtagaggc tgttcgggga gcggcgcccg aggacggtga cgccgccctt
     2461 gtagagcttc aggcgcgcct cgcccgtgac gggcttctgg atgtcgtcca tgaggttctg
     2521 gagggcgacg cgttcggggg cgaaccagaa gccgttgtag acggacgcgg cgtagcgtgg
     2581 gaccaggtcg tcccggagct gcagcacctg gcggtcgagc gtgagggact cgacggcctt
     2641 gtgggcatgg aacaggagcg tgccgcccgg agtctcgtag cagccgcgcg acttcatgcc
     2701 cacgaagcgg ttctcgacga tgtcgaccct gccgacgcca tgccgcccgg cgatctcgtt
     2761 cgccttggcg agcagcgcga ccggcccgag ccgttcgccg tcgatggcga cggggttgcc
     2821 atgctcgaaa gagacggtga cgacctgagg cgcgtcaggg gccgcctccg ggtcggccgt
     2881 cagcttccac atgccggccg gcggctgggc ccacgggtcc tcgagtacgc cgccctcgta
     2941 ggagatatgg aagaggttcg cgtccgtgga gtacggcttc tccttggtca cgggcacgtc
     3001 gatgccgtgc gccttcgcgt acgcgatgca gtcctcgcgg cccttgaggt cccactcgcg
     3061 ccacggcgcg atgacgcgca cgtccggctt gagggcgtag gcggagagct cgaagcggac
     3121 ctggtcgttg cccttgccgg tggcgccgtg ggcgacggcg tcggctccct cggcctccgc
     3181 gatctccacc atcttgcgtg cgatgagcgg ccgggcgaag gacgtgccca gcaggtagta
     3241 gccctcgtag atggcgccgg cgcgcagcac ggggaagacg aagtccctga cgaagtcctc
     3301 ctgcaggtcg acggcgtagg cggcgacggc tccggtgcgg agggccttcg ccttggcggc
     3361 ggcgacttcc tcgccctgcc cgatgtcggc cgtgaacgcg accacctcgg cgccgtactg
     3421 cgcctttatc cagtggagga tgacggaggt gtcgagaccg cccgagtacg ccaggacgac
     3481 cttcttgacc ggcttgcgtt cgttgctcat gctctttcgg ctctccttat gctctcgggt
     3541 ccgcgcctcc ggttacccgc gcgatgctga ggcgaccgac cgccacggaa cgtgccggcc
     3601 atccgctcgg tctggccaat catggccgtg ccggtgcggt ggcggcggcg ttcagctacg
     3661 tcggtctcgt ctgtggttgc gggtgcggag gtggtggggg cgtgaagtgc gggccatcgt
     3721 ggtggatatt cggcaaccag cggatgctta tgcaaaccgg ctgataacgt tgcaacgata
     3781 gcatcggccg gggtggcagt caacggccgc cgctggcgcc ggggccggcg ctcgacggct
     3841 tgcgagccgg gcgccgctaa gcggcgggcc gcccgcgccg ggccgccctg gccggccgcg
     3901 ccggcaccgc ggacagcatg gcggcgccga ccaccaacgc gcctccgacg atgccgagcg
     3961 ttcccagccg ctcgccgaag agccacgcgg ccagtatcgc ggctacgacc ggctctatgg
     4021 tcgccacgag cacggcgcgc gaagcttcca cccgtttcag acccgtgtag tacacgaagt
     4081 acgccaggta ggtggagacc gcggccatca ggaacaggag cagccaggtc ctcggcgtcg
     4141 ggttagtcag cgcggtgaag ggcgtgagcg gcgccaggcc gagcgccccg accggcatga
     4201 cgatggcgta gatggttgcc ggccggtagc gtgccagtac ccacttgccg aacaggtagt
     4261 agctcgagta cgacgccccg gccactaggc cccacgtgac gctccgtacg ctgacggtca
     4321 tgccggactc accgccctgc gccaccaaca cgacccctac gaccgacatg gccacgaggg
     4381 cggacttggt cggcgtgagc cgctcgccga ggaggagcgc ggccaggacc gcgacgaacg
     4441 cgggcgcgga gtagaggagg atgaacgcga ggctgacgcc gcccgcctcg atggcgaggc
     4501 ggacggcccg ctcgggcccc gcgtccaggt gaacgccgcc ggctccgcct tcaccaacgt
     4561 cgtcctctcc cagagccgcc ccgtggatgt cgacggcgac gggatcgtcg agatcctcgc
     4621 ggcgcgcaat ccgccgggcg agtacc
//
LOCUS       DAOQYE010000040         4636 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_18903_length_4636_cov_3.223969, whole genome shotgun sequence.
ACCESSION   DAOQYE010000040 DAOQYE010000000
VERSION     DAOQYE010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 4636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4636
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18903_length_4636_cov_3.223969"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="PKN52_01365"
     CDS             complement(<1..579)
                     /locus_tag="PKN52_01365"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179232.1"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HNQ98615.1"
                     /translation="MHDVVITTAIRTPVGRAHKGLLKDTRPDDLAALVIREALARTPG
                     LAPERVDDVLVGNAHPEGEAGYNLARIASLIAGLPDGAAGATINRFCASGLQTIVQGV
                     HSVAGGMNEVVLAGGSDCTSRVPMGGSDLPLNRELVRSRPGAYHPMGQTAEAVARRYG
                     ITREQQDRFALASHAKALAARAAGRFAEQVVPV"
     gene            complement(667..1233)
                     /locus_tag="PKN52_01370"
     CDS             complement(667..1233)
                     /locus_tag="PKN52_01370"
                     /inference="COORDINATES: protein motif:HMM:NF015716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HNQ98616.1"
                     /translation="MKARGIRGGILLSLEQGDDVAGVSAMLEAHAELLVDKVSVEIAE
                     RTPHALIEAIAGQVASAGGTLVDVRPPTAVMQARGETVIVARTVRSGGKIDSTGSVVV
                     LGDVNAGAEIIANDDIIVVGTLRGLAHAGANGNDRAVIWARRILSPQLRIGNALAQAG
                     GEDGLEENGPEVAHLKDGAIVIRPWHTN"
     gene            complement(1230..3035)
                     /locus_tag="PKN52_01375"
     CDS             complement(1230..3035)
                     /locus_tag="PKN52_01375"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNQ98617.1"
                     /translation="MMQGRMRVLLAGMLTVLALYTARLMYLQLVMVEEYTARSVQNAT
                     QQRHIVPLRGRILARDGTVLADDRVAYDLLYRGGPISDWDHLAALLGLSGPPRQPDRT
                     KAEEVQNGAVIAWNIPDQLVSAVEERVAGSSNLYLRERLERTYPTNLAAQTIGYTAQA
                     DPVRNPGLSNNDLVGVMGLEAGLDDILFGAPGTRQVEVDNRGTEVASQVVLTAQPGQD
                     VVLTLDPQIQRLAEDVLAGSLQYVNTDRRRVGLPTEDVLRGAIIALDPRTGEILAMAS
                     APTFDQNVFTRRPSDPEAVTAILTDSKHVPLANRAVEAYPPASTFKIVTSSALLEKGY
                     ISPNSVYQCSASFSFGGGTWRNWDTYGKGGYTVKQAIADSCNTFFWHAAAVTPSFGVG
                     WAPLIADVVERAHELGFGVKLDVGLPEEKTGRVPDRDWVRSQPQYEHGWLPGFTLNTI
                     IGQGDTLATPLQIAQLISTVALNGHQVEPHLIARVGDEVFEPRVTDIPGDFYGTLREG
                     MRMMLTDFPSRSLLGPGAFPVSVAGKTGTAQTSRGGDYTHAWFMGFSPYEAPEVAVVV
                     FIEYGGSGSRVSVPIARDFLAGYWRLRGVEVPVKP"
     gene            complement(3036..3527)
                     /gene="mreD"
                     /locus_tag="PKN52_01380"
     CDS             complement(3036..3527)
                     /gene="mreD"
                     /locus_tag="PKN52_01380"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HNQ98618.1"
                     /translation="MRLVVFYLILLVAQGFLSSMLAPIPAPDLFLLAVLTLLWRIQPW
                     QLVLVAYGVGLLQDVVGSGVLGAHAFALAAAALAGSMVRAQLSNTGVLERVLVVFVAS
                     LGKWAVMLGVVVWLSGGALGLGRAAMVGLVESVLTVAAGLLVLPWSAALLERSRVLQK
                     ELL"
     gene            complement(3556..>4636)
                     /locus_tag="PKN52_01385"
     CDS             complement(3556..>4636)
                     /locus_tag="PKN52_01385"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HNQ98619.1"
                     /translation="YRRSKARIPIVLGRSIDGDMMVGDLTRMPHLLIAGSTGSGKSVA
                     VNTLISSLLYRFLPTEMRLLMIDPKMVELTPYDGIPHLLRPVVTNPADAAGVLLGTVT
                     HMERRYKMMSKVGAKNLDQYNEKAKTLDLPPLPFIAIIIDELADLMITSPKEVESAIM
                     RLAQMARATGMHLILATQRPSVDILTSLIKVNVPARMAFAVSSSHDSRTILDSMGAER
                     LIGMGDMLYYQPGLVKAVRLQGPFVSENEIGRLADFLRRQYFDDDFVEAYGADFDPPP
                     ADESTATGLIDWNDDKLRAAAELVINEKQASVSRLQRRLSVGHARAGKLMDSLEALGV
                     VGPHQGSKPRDVLVDLAELPNIFGK"
BASE COUNT          675 a         1559 c         1690 g          712 t
ORIGIN      
        1 caccggcacg acctgctcgg cgaagcgtcc ggccgcgcgg gccgccagcg ccttcgcgtg
       61 gctggcgagg gcgaagcggt cctgctgctc gcgcgttatc ccgtagcgcc gcgccacggc
      121 ctcggccgtc tggcccatgg ggtgataggc gcccggcctg ctccgcacca actcgcggtt
      181 gagggggagg tcgctgccgc ccatgggcac gcggctcgtg cagtcggagc cgccggccag
      241 cacgacctcg ttcatgccgc cagccacgct atgcacgccc tgcacgatcg tctggaggcc
      301 cgaggcgcag aagcggttga tggtggcgcc ggccgccccg tcaggcagcc ctgcgatgag
      361 gctcgcgatg cgcgccaggt tgtagccggc ctcgccctcc gggtgggcgt tgccgacgag
      421 cacgtcgtcg acgcgttccg gggcgagccc cggcgtgcgc gccaaggcct cgcggatgac
      481 gagcgccgcc aggtcgtcgg gccgcgtgtc cttgaggagc cccttgtggg ccctgccgac
      541 cggggtgcgg atggccgtcg tgatgactac gtcgtgcacg gtcttctcct gtgtctggaa
      601 gcgcgcgggc gaccgccggt cgggcgtcgg cgccgggcca gcgccaccgg ttgggtgagc
      661 cgcgtctcag ttcgtgtgcc aggggcggat gacgatggcg ccgtccttga ggtgcgccac
      721 ctccggtccg ttctcctcga gaccgtcctc gccgccggcc tgcgccagtg cgttgcctat
      781 gcggagttgc gggctaagga tgcgccgcgc ccagatcacg gccctgtcgt tgccgttcgc
      841 accggcgtgc gccaggccgc gcaacgtgcc taccacgatg atgtcgtcgt tggcgatgat
      901 ctccgcgccg gcgttgacat ccccgaggac gacgaccgac cccgtcgagt cgatcttgcc
      961 gccgctccgc acggtgcgtg cgactatcac cgtctcgccc cgcgcctgca tgacggcggt
     1021 gggcggccgc acgtcgacga gcgtgccgcc ggcggaggcc acctgccccg cgatcgcctc
     1081 gatgagcgcg tggggcgtgc gttctgcgat ctcgacggac accttgtcga ccagcagctc
     1141 ggcatgcgcc tctagcatgg ccgatacccc ggccacgtcg tcgccttgct cgaggctcag
     1201 caggatccca ccacgaatcc ctctagcttt catggtttta ccggcacctc cacgccgcgc
     1261 agccgccagt accccgccag gaagtcccgc gcgatgggga ccgagacgcg tgagccggag
     1321 ccgccgtact cgatgaatac cacgaccgcg acctccgggg cctcgtaagg tgagaacccc
     1381 atgaaccagg cgtgggtgta gtcgccgccg cgcgacgtct gcgccgtgcc cgtcttgcct
     1441 gcgacgctca cggggaacgc gccgggaccg aggaggctgc gcgacgggaa gtccgtgagc
     1501 atcatgcgca tgccctcgcg tagggtgccg tagaagtcgc cggggatgtc ggtcacgcgc
     1561 ggttcgaaga cctcgtcgcc gacgcgcgcg atgaggtggg gctccacctg gtgcccgttc
     1621 agggcaacgg tggagatgag ctgcgcgatc tggagcggcg tggcgagagt gtcgccctgg
     1681 ccgatgatgg tgttgagcgt gaagccgggc agccagccgt gctcgtactg gggctgcgac
     1741 ctcacccagt cgcggtcggg cacgcggccc gtcttctcct cggggagccc gacgtcgagc
     1801 ttgacgccga agccgagctc gtgcgcgcgc tcgacgacgt cggcgatgag gggcgcccag
     1861 ccgacgccga agctgggagt gacggcggcc gcgtgccaga agaacgtgtt gcaactgtcg
     1921 gcgatggcct gcttgaccgt gtagccgccc ttgccgtacg tgtcccagtt gcgccacgtg
     1981 ccgccaccga aactgaagga ggccgagcac tggtagaccg agttcggcga gatgtagccc
     2041 ttctcgagga gcgcggagga cgtgacgatc ttgaacgtcg aggcgggcgg gtacgcctcc
     2101 accgcgcggt tggcgagggg cacgtgcttg gagtcggtga ggatggcggt cacggcctcc
     2161 gggtcggagg ggcggcgcgt gaagacgttc tggtcgaacg tcggcgcgct cgccatggcg
     2221 aggatctcgc ccgtgcgcgg gtcgagcgcg atgatggcgc cgcgcagcac gtcctcagtc
     2281 ggcaggccga cgcgccggcg gtcggtgttg acgtactgca acgagccggc gaggacgtcc
     2341 tcggccaacc gctggatctg cgggtcgagc gtgaggacga cgtcctgccc cggctgggcc
     2401 gtcagcacta cctgcgacgc cacctcggtc ccgcggttgt cgacctcgac ctgccgcgtg
     2461 cccggcgccc cgaagaggat gtcgtcgagg cccgcctcga ggcccatcac ccctaccagg
     2521 tcgttgttcg acagcccggg gttgcgcacc gggtcggcct gcgccgtgta gccgatggtc
     2581 tgggcggcca ggttggtcgg gtaggtgcgc tcgaggcgtt cgcgcaggta gaggttcgag
     2641 ctgcccgcga cgcgctcctc gacggcgctc acgagctggt cggggatgtt ccaggcgatc
     2701 acggcgccgt tctgcacttc ttccgccttc gtgcggtccg gttggcgcgg ggggccgctc
     2761 agcccaagga gggcggcgag gtggtcccag tcggagatcg ggccgccgcg gtagagcagg
     2821 tcgtaggcga cgcggtcgtc ggccagcacg gtgccgtcgc gcgccaggat gcgcccgcgc
     2881 agggggacga tgtggcgctg ctgcgtcgcg ttctgcacgg agcgcgccgt gtactcctcg
     2941 accatgacga gctgcaggta catgaggcgc gccgtgtaca gggcgaggac cgtcagcatt
     3001 ccggccagca gcacgcgcat gcgcccctgc atcatctaga ggagctcctt ctgcaggacg
     3061 cggctccgct cgagcagggc ggcgctccag ggcagtacga ggaggccggc ggccaccgtc
     3121 aggacgctct cgacgagccc caccatggcg gcccggccga ggccgagcgc gccgccggag
     3181 agccacacga ccacgccgag catgacggcc catttaccga ggctggcgac gaagacgacg
     3241 agaacgcgct caagcacccc cgtgttggag agttgcgccc tcaccatcga gcccgccagg
     3301 gcggcggccg cgagggcgaa cgcatgcgcc cccagcacgc cgctgccgac gacgtcctgc
     3361 agcagcccga ccccgtaggc gacgagcacg agctgccacg gctggatgcg ccacaggagc
     3421 gtcagcacgg ccaggaggaa gaggtcaggc gccgggatgg gcgccaacat ggaagacagg
     3481 aagccctgcg ccaccaggag gatcaggtag aagaccacga ggcgcacggt gcgagtctac
     3541 ccgaccctcc gggcactact tgccgaagat gttaggcagc tcggcgagat cgaccagcac
     3601 gtcacggggc ttgctcccct ggtgcgggcc gaccaccccg agcgcctcga gcgagtccat
     3661 gagcttccca gcgcgcgcat ggccgacgga gaggcggcgc tgcagccgcg acacgctcgc
     3721 ctgcttctcg ttgatgacga gttcggccgc cgcgcgcagc ttgtcgtcgt tccagtcgat
     3781 gaggccggtg gccgtcgact cgtcggccgg cggcgggtcg aagtccgcgc cgtacgcctc
     3841 cacgaagtcg tcgtcgaagt actggcgcct gaggaagtcg gccagccgcc ctatctcgtt
     3901 ctcactcacg aacggcccct gcaggcgcac ggccttcacc aggccaggct ggtagtagag
     3961 catgtcgccc atgccgatca ggcgctcggc gcccatggag tcgaggatcg tgcgcgagtc
     4021 gtggctgctg gagacggcga acgccatgcg cgccggcacg ttcaccttga tgaggctcgt
     4081 caggatgtct accgagggcc gctgcgtggc gaggatcagg tgcatgccgg tggcgcgcgc
     4141 catctgggcg aggcgcatga tggcggactc gacctccttc gggctcgtga tcatgaggtc
     4201 ggcgagctcg tcgatgatga tggcgatgaa gggcagcggc ggcaggtcga gcgtcttggc
     4261 cttctcgttg tactggtcga ggttcttggc cccgaccttc gacatcatct tgtagcgccg
     4321 ctccatgtgc gtgaccgtcc cgagcaggac gccggcggcg tctgccgggt tggtcaccac
     4381 cggcctgagc aggtggggga ttccgtcgta aggggtcagc tccaccatct tagggtcgat
     4441 catcaggagg cgcatctccg tggggaggaa gcggtagagc agcgacgaga tgagcgtgtt
     4501 cacggcgacg gacttgccgg aaccggtgga gccggcgatc agcaggtgcg gcatgcgtgt
     4561 caggtcgccc accatcatgt cgccgtcgat ggagcggccg agcacgatgg ggatgcgcgc
     4621 cttggagcga cggtag
//
LOCUS       DAOQYE010000041         4570 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_19264_length_4570_cov_3.682835, whole genome shotgun sequence.
ACCESSION   DAOQYE010000041 DAOQYE010000000
VERSION     DAOQYE010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 4570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4570
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19264_length_4570_cov_3.682835"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1202
                     /locus_tag="PKN52_01390"
     CDS             <1..1202
                     /locus_tag="PKN52_01390"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNQ98620.1"
                     /translation="GGEPVSPAAGAAAGADAAATAGATARFAVHDLRTWTSTEVADPG
                     TIPLDLAALVYTSGSTGNPKGVMLSHQNMVFAQGSLVEYLRLDASDRILDLLPLAFDY
                     GLYQALMAVHLGATLVLERTFAFPAAIVKRVVEEGVTVFPGVPTVFATLLSLHRNSPI
                     AMPSVRRYTNTAAHLPDEYVPGLLEMSPGALVYKMYGLTECKRVCYLEPELVLSKPSS
                     VGKAIPGTETYLLGEDGHPVGPGVTGILHVRGPHVMLGYWNLPAETEHMLKPGKYPGE
                     RVLCTHDWFRKDEDGFLYFMGRSDDIIKSRGEKVSPVEVENALAAIPGVREAAVIGVP
                     DELLGQAVRAFVVLDPGASYTEQAFKREALARLESFMVPRDVRFVDDLPKTATGKVRK
                     KSLVEQG"
     gene            1281..1547
                     /locus_tag="PKN52_01395"
     CDS             1281..1547
                     /locus_tag="PKN52_01395"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNQ98621.1"
                     /translation="MPDLTTIDRDVRQFLADNFILDNSGADLGADESLTQAGVLDSMG
                     VLELIMFVEERFGVSIPDEDTLPENLDSVARIVAYIGRRLAQGA"
     gene            1553..2539
                     /gene="nadE"
                     /locus_tag="PKN52_01400"
     CDS             1553..2539
                     /gene="nadE"
                     /locus_tag="PKN52_01400"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF002048.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HNQ98622.1"
                     /translation="MAEAFGPHVLRVADPEAAITAITEGMRREVRAFRRRGGVLGLSG
                     GIDSTVVAALAARAFGPGNVLGVLMPEADSSEDTLRLSRLAASSAGIETAMEEITPIL
                     DAVGCYRRRDEAFKRVIPEYGPGWKAKIVLPSVVESESFRLFSVVALAPDGRTLKERL
                     PLEDYLQVVAATNFKQRTRKMLEYYHADRLNYCVLGTPNRLEYDQGFFVKNGDGAADV
                     KPIAHLYKSQVYQLAEALGVPEEIRSRPPTTDTYSMPQSQEEFYFSLPYDKMDLCLYA
                     VDHGVGADAVAASLGLTAEQVDRVFKDIAGKRRVSAYLHAAPRLAVEGPAGA"
     gene            2551..3654
                     /locus_tag="PKN52_01405"
     CDS             2551..3654
                     /locus_tag="PKN52_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98623.1"
                     /translation="MRIRPFEPDDAAGVASLWQYWFRGKTRVPDPGLVELVRRMYVEY
                     PSVDAEVRPLVAEDERGKLLGFLGVTAMPVMVDGEERTMAGIFPSVVDPEAPTTVAAF
                     LLRKFLAGPQVFTISDGGHVKFERIWELLGGSIAQMQSLRWVKVFRPGALGLEVVADR
                     NKVLRALAPLLGPIVRGGDVAARHLARSRLVNGPVANYRSEPLTPGALARHAPELLGH
                     SRLWPVYSDRHVAWQFEQMARIVQQGGFRARIVMHGEEVVGWYIYYLRKGGICRLFDL
                     EATPNHMDAVVGQLFAEADEVGAGALIGRMEPRLRGSMNRHGALVHNGGSLLMVHSKD
                     PSLVTDALLGRLAFSRLQGENWYWWAINSRVVP"
     gene            complement(3670..>4570)
                     /locus_tag="PKN52_01410"
     CDS             complement(3670..>4570)
                     /locus_tag="PKN52_01410"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNQ98624.1"
                     /translation="VVRALLARGAHVNVLDDFSTGKRENLPASDDLRVIEGDVADPSA
                     VRSALEGRDAFIHLAAVASVELSVREPLATHRTNLEGSIQLFEAAARLGVRRGLYASS
                     AAVYGDYANLPLATTEAPRPLSPYAADKLAGEHYLAFYHRGGRLNATAFRFFNVFGPR
                     QDPASPYSGVISIFLDRARRGAPITVYGDGEQTRDFVYVGDVVAALLAALDRQGDGAG
                     APPELPIYNVGRGERVSLLQLLATIERLPGIQAPLTVSHGPAREGDIRHSLADATPLR
                     TVLEWRPRTSLADGLTAILTEEE"
BASE COUNT          726 a         1584 c         1555 g          705 t
ORIGIN      
        1 gcggcggcga gcccgtgagc cccgcggcgg gtgccgctgc gggtgccgac gcagccgcca
       61 ccgcaggcgc cactgcgcgg ttcgccgtcc acgacctgcg gacctggacg agcacggagg
      121 tggccgaccc cggcaccatc ccgctcgacc tcgcagcgct cgtctacacg tccggcagca
      181 cgggaaaccc taagggcgtc atgctcagcc accagaacat ggtcttcgcg caggggagcc
      241 tcgtcgagta cctgcgcctc gacgcgtcgg accggatcct cgacctgctg ccgctcgcct
      301 tcgactacgg gctctaccag gccctgatgg ccgtccacct cggcgccacg ctcgtgctcg
      361 agcgcacgtt cgccttcccc gccgccatcg tcaagcgcgt cgtcgaagaa ggcgtcacgg
      421 tgttccccgg cgtgccgaca gtgttcgcga ccctcttgtc gctgcaccgc aactcgccga
      481 tcgccatgcc gagcgtgcgg cgctacacga acacggccgc ccacctgccg gacgagtacg
      541 tgcctggcct gctggagatg agccccggcg ccctcgtgta caagatgtac ggcctcaccg
      601 agtgcaagcg cgtgtgctac ctggagccgg agctcgtcct ctcgaagccg tcgtccgtcg
      661 gcaaggccat acccggcacg gagacctacc tgctcggcga ggacggccac cccgtcggac
      721 ccggcgtgac cggcatcctg cacgtgcggg ggccgcacgt catgctcggc tactggaacc
      781 tgccggccga gaccgagcac atgctcaagc cgggcaagta ccccggcgag cgcgtgctat
      841 gcacccacga ctggttccgg aaggacgagg acgggttcct ctacttcatg ggccgcagcg
      901 acgacatcat caagagccgc ggggagaagg tcagcccggt cgaggtcgag aacgccttgg
      961 cggccatccc cggcgtgcgc gaggcggccg tgatcggcgt gccggacgag ctgctcgggc
     1021 aggcggtgcg ggcgttcgtc gtcctcgacc ctggagcgag ctacacggag caggcgttca
     1081 agcgcgaggc gctggcgcgt ctggagagct tcatggttcc gcgcgacgtg cgcttcgtag
     1141 acgacctccc caagaccgcc accggtaagg tacgcaagaa gagcctggtg gagcagggct
     1201 gaccgcccgc ccccacggcg cccccgcccc acggccgcgc gagccggcca ttaggaacga
     1261 caaggagcga caccaagaag atgccagacc tcaccaccat cgaccgcgac gtccgccagt
     1321 tcctcgcgga caacttcatc ctcgacaaca gcggcgccga cctcggcgct gatgagtccc
     1381 tcacccaagc tggcgtgctc gactccatgg gcgtgctaga actcatcatg ttcgtcgagg
     1441 agcgattcgg cgtgagcatc cctgacgagg acacgctccc cgagaacctc gacagcgtgg
     1501 cgcgcatcgt cgcctacatc gggcgacgcc tggcgcaggg ggcttgacgc gcatggccga
     1561 agccttcggt ccgcacgtgc tgcgcgtggc cgaccccgag gccgccatca ccgccatcac
     1621 cgaaggcatg cggcgcgagg tccgcgcgtt caggcgcagg ggcggagtgc tcgggctctc
     1681 cggcggcatc gactccacgg tcgtggctgc gctcgcggcc cgcgcgttcg gcccaggcaa
     1741 cgtgctcggc gtcctcatgc ccgaggccga ctcctccgaa gacacgctga ggctcagtcg
     1801 ccttgcggcc tcgagcgccg gcatcgagac tgccatggag gagataacgc ccatcctcga
     1861 cgccgtcggc tgctaccgca ggcgcgacga ggcgttcaag cgcgtcatcc ccgagtacgg
     1921 ccccggttgg aaggcgaaga tcgtcctgcc gtccgtcgtg gagtcggaga gcttccgcct
     1981 gttctcagtc gtggcgctcg cgccagacgg ccgcacgctc aaggagcggc tgccgcttga
     2041 ggactacctc caggtcgtcg cggccacgaa cttcaagcag cgcacgcgca agatgctcga
     2101 gtactaccac gccgaccgcc tcaactactg cgtgctcggc accccgaacc gcctcgagta
     2161 cgaccagggg ttcttcgtca agaacggcga cggcgccgct gacgtgaagc cgatcgccca
     2221 cctctacaag tcgcaggtct accagttggc cgaggcgctc ggcgtgccgg aggagatccg
     2281 gtcgcggccg ccgacgaccg acacctactc catgccacag agccaggagg agttctactt
     2341 ctccttgccg tacgacaaga tggacctctg cctctacgcc gtcgaccacg gcgtcggcgc
     2401 cgacgccgtg gcggccagcc tcggcctcac cgccgagcag gtcgaccgcg tgttcaagga
     2461 catcgccggg aagcgccgcg tgagcgccta cctgcacgcc gcgccgcggc tggcggtcga
     2521 gggccccgcg ggggcatagg gggactggtc atgcgcatcc gcccgttcga acctgacgac
     2581 gccgcaggcg ttgcctcctt gtggcagtac tggttccgcg gcaagacgcg cgtgcccgac
     2641 cccggtctgg tggagctcgt caggcgcatg tacgtcgagt acccgagcgt cgacgccgag
     2701 gtgaggcccc tggtcgccga ggacgagcgc ggcaagctcc tcggcttcct cggcgtcacc
     2761 gccatgccgg tgatggtgga cggcgaggag cggacgatgg ccgggatctt cccgtctgtc
     2821 gtcgatccgg aggccccgac tacggtggcg gcgttcttgc tgcgtaagtt ccttgcggga
     2881 cctcaggtgt tcacgataag cgacggcggg cacgtcaagt tcgagcgcat ctgggagttg
     2941 ctcggcggga gtatcgcgca gatgcaatcg ctgcgttggg tcaaggtctt cagacctggt
     3001 gccctgggcc ttgaggtggt agctgacagg aacaaggtct tgcgagcgct tgcgccgctg
     3061 ctcggtccga tagtcagagg cggcgacgtg gccgcacgtc acttagcgag atcacgcctt
     3121 gttaacggac cagtcgccaa ctaccgttcc gagccgctga caccgggtgc actggcacgc
     3181 catgcaccag aactcctcgg tcactcgcgt ttatggcccg tatatagcga caggcacgta
     3241 gcttggcagt ttgaacagat ggccagaatc gtgcaacagg gcggcttcag agcgcggatt
     3301 gtcatgcacg gcgaggaggt agtcggttgg tacatttact accttagaaa gggcgggatc
     3361 tgccggctct tcgacttgga agcgacgccg aatcacatgg atgccgtcgt cggtcagctc
     3421 ttcgcggagg ccgatgaggt tggcgcaggg gcgctcatcg gccgcatgga gccacgactg
     3481 cgcggctcga tgaaccggca cggcgcgctc gttcataacg gcggctcgct cctcatggtc
     3541 cactctaagg acccgagcct cgtgaccgac gccctcctcg ggcgcctcgc gttcagccgc
     3601 ctccagggcg agaactggta ctggtgggcg atcaactcgc gggtcgtgcc ttagcccttc
     3661 agtagcgccc tactcctctt cggtgagtat cgccgtgagc ccgtccgcca acgaggtgcg
     3721 gggccgccat tcgagcacgg tcctcagggg ggtcgcgtct gcgagcgagt gcctgatgtc
     3781 gccttcgcgc gcggggccgt ggctcaccgt caagggggcc tggatgcctg gcagccgttc
     3841 gatggtggcg agcagctgga gcagcgacac gcgttcgccg cgccccacgt tgtagatggg
     3901 cagctcgggc ggcgcgcccg ccccgtcgcc ctgccggtcg agggcggcca ggagcgcggc
     3961 gaccacgtct cctacgtaga cgaagtcgcg cgtctgctcg ccgtcgccgt acaccgttat
     4021 cggggcgccg cgccgcgccc ggtcgaggaa gatgctgatc acgcccgagt aggggctggc
     4081 cgggtcctgg cgcggcccga agacgttgaa gaagcggaac gcggtcgcgt tcaggcgccc
     4141 gccccggtga tagaaggcga ggtagtgctc gcccgccagt ttgtcggcgg cgtaggggct
     4201 cagcggacgg ggcgcctcgg tcgtcgccag cggcaagttg gcgtagtcgc cgtacacggc
     4261 ggcggaggac gcgtagagac cgcgcctgac cccaaggcgc gccgccgcct cgaagagctg
     4321 gatgctcccc tcgaggttcg tacggtgcgt ggccagcggc tccctgaccg acaactccac
     4381 ggaggcaacg gccgccaggt ggatgaaggc gtcgcgaccc tcgagcgcgg agcgcacggc
     4441 gctcgggtcc gcgacgtcgc cctcgatgac ccgcaggtcg tcggaggcgg ggaggttctc
     4501 ccgcttgccc gtcgagaagt cgtcgaggac gttcacgtgc gcgccgcgcg ccagcagggc
     4561 tcgcacgacg
//
LOCUS       DAOQYE010000042         4524 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_19542_length_4524_cov_3.117476, whole genome shotgun sequence.
ACCESSION   DAOQYE010000042 DAOQYE010000000
VERSION     DAOQYE010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 4524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4524
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19542_length_4524_cov_3.117476"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="PKN52_01415"
     CDS             <1..381
                     /locus_tag="PKN52_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98625.1"
                     /translation="AQTILGEARASVKYREDNDYTLAGNAAGTSTFGVDDGLQVSLNG
                     EVIYDDGGSLSGNRGPITFTAKKGDTLKFVVRDTYGHCSGLQTIYLVKGTLATVADPG
                     FDLGCGRPSGDQGIVHTLEFTIPF"
     gene            567..1781
                     /locus_tag="PKN52_01420"
     CDS             567..1781
                     /locus_tag="PKN52_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98626.1"
                     /translation="MRRRPLSQLTPHLLALSLLVVVIAACGTPTTPPRGGEPQCTDPA
                     SLVTIPDENLRASITTAVRRDTGGSEDLTCANLAMVKQLNIRGATLGSLEGIQHLVNV
                     TRLSFDNSPFPASHATKLEALTKVKDLTFWEVPLDTLAFASKMKELETLSLDQTKVTS
                     LEPLRGLPLLRSIAADHGLITNLDAIGSLPSLTFFRSLGNPVTSLGQLVQSPSLTNLE
                     IENSQLTSLSGITQVKTLRVLRVNGAKLTSVPDLSSMTSLEEVYFARNQLTAVPVLPA
                     VNLGVLVLSYNQLTNLNGIARSPKIAKLLLDNNQLTDLLPLTDLADGIENLDLANNFI
                     WELGPLTVNPNMFAPGAWLYLRLNCLGLNGALPYLYPSNNDAIYTLQARGVNFNYTPF
                     QDEDLCDAVVVP"
     gene            complement(1814..2320)
                     /locus_tag="PKN52_01425"
     CDS             complement(1814..2320)
                     /locus_tag="PKN52_01425"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HNQ98627.1"
                     /translation="MRTVPKVLLILGFVCLLASCATTTPPKGDKDPDPGKPKMAEAML
                     AAVNDVRTEGTTCGGTVAPPVGKLQLNDLLTEAAQGHSDDMYAHETMSHVGSDGSTPD
                     MRIAATGYQATWWGENVAWNQRSVEEVMAAWLSSTGHCVNIMNPNFTEFGVGEAGYYW
                     TQVFGRPL"
     gene            complement(2455..3123)
                     /locus_tag="PKN52_01430"
     CDS             complement(2455..3123)
                     /locus_tag="PKN52_01430"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HNQ98628.1"
                     /translation="MKSARGLLLVFGVAWLLIACDTLDRNVNVANPGAPTDPADPGAP
                     TDPGPGEPQPEPEPVPEPEPEPEPEPEPDPGPDPELEPEPEPDPVPSTTASRLLAAVN
                     GARTTGATCDGVWQAPAAPLALSPLLVQAAQAHSEDMLATKTMSHTGSDGSNPGERIS
                     RTGYKWSTWGENVAAGYRSVEQVMTGWMGSAGHCKNIMNPGFTEFGAGEAGYYWTQTF
                     ARPR"
     gene            3279..3740
                     /locus_tag="PKN52_01435"
     CDS             3279..3740
                     /locus_tag="PKN52_01435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HNQ98629.1"
                     /translation="MPVEPLNLDLKTMLEELIPFNKLLGVRLVSTDAAAGRLVMKLPL
                     RAELIGNAYRGMVHGGAVSALIDAAAGGAAALQLPDLQEALAVATIDMRVDYLEPGRG
                     EFLTAEAQVVRCGRRVIVIRIDVSDPNGTLVAIGTATFSRPTPPRPALGPA"
     gene            complement(3697..>4524)
                     /locus_tag="PKN52_01440"
     CDS             complement(3697..>4524)
                     /locus_tag="PKN52_01440"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNQ98630.1"
                     /translation="KASLAVCMLLVADAAARGLAGDVVLMCVADEEHDSIGTREALAH
                     LARTSRFDAAIVTEVTSLELHVAHRGFALFEVELEGKESHTSKPAEGVNALTHLGRLL
                     AAVERHDEELRRRPPHQLLAHGSLQAVLAEGGQELFTTPSRASASIERRTLPGETAAT
                     TLAELEALLAELTAADPTVKPTVRQVLARSPFEVAPGAEVLELLSVAIEAETGTAPPR
                     IGAPYWTDAALIAEAGIPTVLFGPTGGGIHQLDEWVDLWSVHAVMRVLRRVVGELAG"
BASE COUNT          737 a         1617 c         1451 g          719 t
ORIGIN      
        1 gcgcagacga tcctcggcga ggcgagggca agcgtcaagt accgcgagga caacgactac
       61 accttggcgg gcaacgccgc cggtaccagc accttcggcg tcgacgacgg gctgcaggtc
      121 agcctgaacg gcgaggtcat ctacgacgat ggcggctcgc tctccggtaa ccgcgggccc
      181 atcacgttca ccgccaagaa gggcgacacc ctcaagttcg tggtgcgcga cacctacggc
      241 cactgcagcg gcctacagac catctacctc gtgaagggca ctctagccac cgtggccgac
      301 cccgggttcg acctcggctg cgggcgaccg agcggcgacc aggggatcgt gcatacgctc
      361 gagttcacga tcccgttctg aggtcctctg gccaacgagg ttcgccgcct ctgaggtaag
      421 cctttccgag atccttgcgc tcgataccag ggtccccgcc gacagtggcg gggacctacc
      481 cggtttaagg ctgggttaaa cacgccgggg tcagactggc gcaaacgacg atccattgca
      541 gtcgtcagtg ccgttggagg cccgagatgc gccgccgtcc cctcagccag ctaacccccc
      601 acctgctcgc tctctcgctg ctggtcgtgg tcatcgccgc gtgcgggact ccgactacgc
      661 ccccgcgcgg gggcgaaccg cagtgcaccg accccgcctc gctcgtaacg atcccggacg
      721 agaacctgcg cgccagcatc acgacagccg tgcgccgcga tacgggcggg agcgaggacc
      781 tcacctgcgc gaacctcgcg atggtgaagc agctgaacat ccggggcgcg acgctcggct
      841 cgctcgaggg gatccagcac ctcgtcaacg tcacgcggct gagcttcgac aacagcccct
      901 tccccgcgtc ccacgcgacg aagctcgagg ccctgacgaa ggtcaaggac ctgacgttct
      961 gggaagtgcc tctggacacg ctcgcgttcg cttccaagat gaaggagctc gagacgctgt
     1021 cgctcgacca gacgaaggta accagccttg agccgctgcg gggcctgccg ctcctcagga
     1081 gcatcgcggc ggaccatggc ctgatcacca acctggacgc gatcggcagt ctgccgtccc
     1141 tcacgttctt ccgctcccta gggaacccgg tgacgagcct aggtcagcta gtgcagtcgc
     1201 catcgctcac caacctggag atcgagaaca gtcagttgac gagcctcagc ggcataacgc
     1261 aggtcaagac cctgcgcgtg ctgcgcgtga acggcgccaa gctcacgagc gtgccggacc
     1321 tcagctccat gacgagcctc gaggaggtct acttcgcccg caaccagctc accgccgtgc
     1381 cggtcctgcc ggccgtgaac ctgggcgtgc tcgtactcag ctacaaccag ttgaccaacc
     1441 tgaacggcat agctcggagc cccaagatcg cgaagctcct cctcgacaac aaccagctca
     1501 ccgacctgct gccgctcacc gacctagccg atggcatcga gaacctcgac ctcgccaaca
     1561 acttcatctg ggagctgggg cctctgaccg tgaaccccaa catgttcgcg cccggcgcct
     1621 ggctgtacct gcggctgaac tgcctcggcc tcaacggggc gttaccctac ctctacccca
     1681 gcaacaacga cgcgatctac acgttgcaag cgcgcggcgt gaacttcaat tacacgcctt
     1741 tccaggacga ggacctgtgc gacgccgtgg tggtgccgta gggtcatagg ggacgcctcg
     1801 taccggctcg tcgctagagg ggccgcccga acacctgggt ccagtagtaa ccggcctcgc
     1861 ccacgccgaa ctcggtgaag ttcgggttca tgatgttgac gcagtggccc gtgctgctca
     1921 gccacgcggc catgacttcc tcaacgctcc tctggttcca agccacgttc tcgccccacc
     1981 aggtggcctg gtagcccgtc gcggcgatgc gcatgtccgg agtgctgccg tccgatccga
     2041 cgtgggacat ggtctcgtgc gcgtacatgt cgtcgctgtg gccctgcgcc gcctcggtga
     2101 ggaggtcgtt gagctgcaac ttgccgaccg gcggagccac ggtgccgcca cacgtagtgc
     2161 cctccgtcct cacgtcgttg accgcagcca acatcgcctc cgccatcttc ggcttacctg
     2221 gatccggatc cttatcgccc ttggggggcg tcgtcgtcgc gcaactcgcc aacagacaca
     2281 cgaacccgag tatcagcagc accttcggaa ccgttcgcat gcgcgaatag tactcgcggc
     2341 cagggcggtc cgccgggcgg atcgcacata accggcaaga tcggtcggga gacacttcgg
     2401 acgtggcgcg ttcaagcacc agttaagcgt gaagcagggg cgcaccgttc ggcgctatct
     2461 gggccgcgcg aaggtctgcg tccagtagta gccggcctcg ccggcaccga actcggtgaa
     2521 gcccgggttc atgatgttct tgcagtgccc ggcgctgccc atccacccgg tcatgacctg
     2581 ctcgacgctc cggtaacccg ccgccacgtt ctcgccccac gtgctccact tgtagccggt
     2641 gcgcgagatg cgctcgccgg ggttgctgcc gtccgagccc gtgtgcgaca tcgtcttcgt
     2701 ggccagcatg tcttcgctgt gggcttgcgc cgcctgcacc agcagggggc tgagcgcgag
     2761 aggagccgcc ggcgcctgcc agacgccgtc acacgtggcg ccggtggtgc gggcgccgtt
     2821 caccgcggca agcaaccggc tcgcggtggt agacggcacc ggatccggct cgggttcagg
     2881 ctcgagctcg gggtcgggcc cagggtctgg ttcgggctca ggctccggtt cgggctcagg
     2941 ctcgggcacc ggctctggct cgggctgcgg ctcgccagga ccagggtcgg tcggagcgcc
     3001 agggtcggcg gggtcggtcg gagcgccggg gttcgccacg ttgacgttgc ggtcgagcgt
     3061 gtcgcacgcg atcaggagcc aagccacgcc gaagacgagc aagagaccac gggcggattt
     3121 cacgttcgaa tggtaggcgg ccgaccggtc aagagctatg cgattcgccc gtccagcctg
     3181 gcgcctcacc cgtccagccc ggcgcttcgc tcggccgcag cgttacgcct acgcgcgacc
     3241 acgagccacg tggcagcgac cagcgatagc atgaccgcat gcccgtcgaa ccgctgaacc
     3301 tcgacctcaa gacgatgctc gaggagctga tcccgttcaa caagctgctt ggcgtgcggc
     3361 tcgtgtccac cgacgccgcg gccggtaggt tggtcatgaa gctgccgctg cgcgcggagc
     3421 tcatcggcaa cgcctaccgc ggcatggtcc acggcggggc cgtatccgcg ctcatcgacg
     3481 cggccgcggg cggcgcggcg gcgctacagc ttcccgactt gcaggaggct ctcgccgtcg
     3541 ccaccatcga catgcgcgtc gactacctcg aaccgggccg cggcgagttc ctcacggccg
     3601 aggcacaggt cgtgcggtgc gggcggcgcg tgatcgtcat ccgcatcgac gtgagcgatc
     3661 ccaacggcac gctcgttgcc atcgggacgg ctactttcag ccggccaact cccccacgac
     3721 ccgccttagg acccgcatga ccgcatgcac gctccagagg tcgacccact cgtccagctg
     3781 atggatgccc ccgcccgtcg gcccgaacag cacggtcggg atgccggcct cggcgatcaa
     3841 cgcggcgtcg gtccagtacg gcgcgccgat cctggggggc gcggtgcccg tctcggcctc
     3901 aatggccacg ctgagcagct ccaacacctc ggcgccgggc gcgacctcga acggcgagcg
     3961 cgccaggacc tggcggacgg tcggcttcac ggtcgggtcc gcggccgtca gctccgctag
     4021 cagcgcttcc agctcggcca acgtcgtggc ggccgtctca ccgggcagcg tgcgccgctc
     4081 gatgctcgcg ctcgccctgc tcggcgtggt gaacagctcc tgcccaccct cggccagcac
     4141 cgcctgcagc gagccgtgcg cgagcagctg gtgaggagga cggcggcgca actcctcgtc
     4201 gtggcgctcc accgccgcaa gcagcctgcc gaggtgcgtt agcgcgttca cgccctccgc
     4261 cggcttggac gtgtgcgact ccttgccctc gagctcgacc tcgaacagcg cgaagccgcg
     4321 gtgcgccacg tgcagttcca ggctcgtgac ctcggtgacg atggcggcat cgaacctaga
     4381 cgtgcgcgcc aggtgcgcta gggcctcgcg tgtgccgatg ctgtcgtgct cctcgtcggc
     4441 aacgcacatg aggacgacgt cgcccgcgag gccgcgcgcg gcagcgtcgg ccaccaacag
     4501 catgcatacg gccaggctcg cctt
//
LOCUS       DAOQYE010000043         4509 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_19608_length_4509_cov_3.055905, whole genome shotgun sequence.
ACCESSION   DAOQYE010000043 DAOQYE010000000
VERSION     DAOQYE010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 4509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4509
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19608_length_4509_cov_3.055905"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            19..273
                     /locus_tag="PKN52_01445"
     CDS             19..273
                     /locus_tag="PKN52_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98631.1"
                     /translation="MRKLRSIATALILTCTVAGIAVGSLAVYHPTSNVVGQTSNQPGE
                     YDVGQTSNQPGEYDVGQTSNQPGEYDVGQTSNQPGEYDVG"
     gene            402..1223
                     /locus_tag="PKN52_01450"
     CDS             402..1223
                     /locus_tag="PKN52_01450"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNQ98632.1"
                     /translation="MRPRLEELCFLYQPVAPVTPGAGPWCEALVRWNHPDGTVRGPLE
                     ILPYWLSQARIEEFTQFTLLRGAQALAANPGARLSVNLSPEQVMLPSTVLTLEGMLRS
                     VTARLHIELTEECHANQAALARQTALLRERCGIVVLDDVAPNDLRDRLRFNAPIDGVK
                     VDRSVVTAALYQTGEVCETARLFIQSLCDTYEIVVAEGIEEPSASVDLQALGVSHVQG
                     FGIARPAAELRTVIGALVAGQDAVPTGAGASVGAGATSARNGSAAGSGLRTRPVR"
     gene            1519..2838
                     /locus_tag="PKN52_01455"
     CDS             1519..2838
                     /locus_tag="PKN52_01455"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HNQ98633.1"
                     /translation="MTSFSFAHLADLHLDTPFTGVRSRAPELARVLRDASLDAWDRAV
                     ALCLEAGVDFVLLAGDIYDGAAVGVRAQLRFRDGLRRLSDAGVASFVVHGNHDPRGGR
                     WTAVADWPERVTVFGHHEVTAAYALRDGVPVALVHGLSHAGPNVTENLAARFTRDPSF
                     GGFQVGLLHANIDRRGGNENYAPATLSDLRHAGLDYWALGHVHARAVLSTGRPLAIYP
                     GNLQARDPGEAGTKGFYVVEVADGEPTARFVSAGVVNFLTVDVDIGADLADLDAVRER
                     LVQAALGASEDASAVSGTTDSGGATVGLKAQHVVMRARVRGMGPAHDGLRKAGEVGLL
                     LYLQDVAPDGVWWDEVRISTRPPLDRAARMAAGDFVADLLEVSMERRHEAPDTVGRLL
                     DELLGNRSLQAVRQELGLDRLLAEADDIWDEAETLAFDLLSDAGSGA"
     gene            2842..>4509
                     /locus_tag="PKN52_01460"
     CDS             2842..>4509
                     /locus_tag="PKN52_01460"
                     /inference="COORDINATES: protein motif:HMM:NF024904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNQ98634.1"
                     /translation="MYLGRVEVHEFRGLKDVEFDLAPSAKPGLVVLEGPNEAGKSTLL
                     AFVRAILFAERGQGESLHGSVTLHDGAKSYRLERNGARSTSRLLDTQTGTRLDLAQLD
                     RLVGNLDGNVYRNVFAFGPSELQSFDALTDGAVRERLYSAAVAGAGRNASAATAHLEA
                     VTTELLKPRAQSVLGDLAQELAEARNGASRARSEARRYAQLASELAATGSEADSLRRD
                     QREATATALWYEKLLQLWPDWSGRAAALDDLRRLSLEEEALNAPGAGGTPPSTWLRAA
                     GAIRDLAADLEAYRRAKADAAELEAEQRDLVEASLNGLRSLGPEWGPPRLAAFADAGA
                     WNARTEVARAAAAAAATALDKVEVALETARQELSAAERGLALATAKVDTWATEAGRPA
                     HGPAADPRKALETLLARARERRLGIATLLDRFDDLNAARSRHADLLAKLQQVGERTTA
                     ALARRWVAFGLTILAAAAVAVIEWEGRPAVAGVAAALGVLLGLLLWPPRGLSAADSAV
                     AELRANAAEASLDVGRSEAALGDARARLGLAPDAGAPEARAAAREVED"
BASE COUNT          723 a         1567 c         1583 g          636 t
ORIGIN      
        1 cataggaggc ttcccagcat gcgcaaactc aggagcatcg ccaccgccct catcctcacc
       61 tgcaccgtcg ccggtatcgc cgtcggcagc ctcgccgtct accatccgac cagcaacgtc
      121 gtgggtcaga ccagcaacca acccggcgag tacgacgtcg gacagaccag caaccagccc
      181 ggggagtacg acgtcggaca gacgagcaac caacctggcg aatacgacgt cggtcagacc
      241 agcaatcaac ccggcgagta cgacgtcggc tgagcgcctg gtcgggctcc gcccgagcgc
      301 acgcacaggc agagcgctct tgaagaagtg cgggagtctc ctagaccgcg acagcaagac
      361 caagaagccc ccggggttga agacagagtg tcatccttag aatgcgacca cgtctagaag
      421 agttgtgttt cctttaccag ccagtagccc cggtgacacc aggcgcggga ccctggtgcg
      481 aggccttggt cagatggaac cacccggacg gcacggtacg ggggcccctc gagatcctgc
      541 cgtattggct ctcccaggca cgcatcgagg agttcaccca gttcacgctc ctgcgcgggg
      601 cgcaggcgtt ggccgccaac ccaggcgcgc gcctctcggt gaacctgagc cctgagcaag
      661 tcatgctgcc gagcaccgtc ctcacgctcg agggcatgct caggagcgta accgcccggt
      721 tgcatatcga actcaccgag gagtgtcatg cgaatcaggc cgctctggct cgccaaacgg
      781 cgttgctgcg tgagcggtgt gggatcgtcg tactcgacga cgtcgctcct aacgacctcc
      841 gcgatcggct gcgcttcaac gctccgatag acggcgtcaa ggtagatcgc agcgtggtca
      901 cggcggcgct ctaccagaca ggcgaggtgt gcgagactgc ccgtctgttc atccagtccc
      961 tctgcgacac ctacgagata gtggtcgccg aaggtataga agaaccgtcc gcaagtgtgg
     1021 acttgcaggc acttggcgtc agccatgtgc agggcttcgg gatagccaga cccgcggccg
     1081 agctgcggac ggtcatcggt gccttggttg cgggccaaga cgccgtgccg acgggcgcgg
     1141 gggcatccgt cggcgccggt gcgacatctg ccaggaacgg ctcggccgct ggctcagggt
     1201 tgcgcaccag gccggtccgc tgaggcgtcg acgccttcct ccagcgttcg acgcctcgct
     1261 cgccgcgtcg gtccggcgcg ttcagcggga ttctcatacg ggccgcgcac caggcgcgtc
     1321 ccgggggtcg aagagggagc acgaagggtg aggccgcaag gcgcctcgcc ccaggggtag
     1381 gagcaggcgg cgttcgcttc agtgggcgag cgccgcctga acttgctcac gccccccggc
     1441 aggcaccgcg acctccaggg ggatatcctc gtaggcgacc gtgtgcgaag gaaccttctc
     1501 agcggatgtc gaggcagcgt gacgagcttc agcttcgcgc atctggccga cctgcacctc
     1561 gacacccctt tcacgggtgt tcgttcccgc gcgccggagc tcgcacgggt gctccgcgac
     1621 gcttcgctcg acgcgtggga ccgcgcggtg gccctctgcc tcgaagccgg ggtcgacttc
     1681 gtcttgctcg ctggcgacat ctacgacggc gctgccgtgg gggtgagagc ccaactacgt
     1741 ttccgggatg gactccggcg cttgtcggat gccggggtcg cgagcttcgt ggtgcatggc
     1801 aaccacgacc cacgcggagg ccggtggacg gccgtagccg actggcccga gcgcgtgacg
     1861 gttttcggcc accacgaggt cactgcggcg tacgcgcttc gcgacggcgt accggtcgcg
     1921 ctcgtgcacg gcctctctca tgcgggcccg aacgtcacgg agaacctcgc cgcgcgcttc
     1981 acgcgcgatc cgagcttcgg tgggttccag gtcggcctcc tgcatgcgaa catcgaccgg
     2041 cgcggcggca acgagaacta cgccccggcg accctctccg acctgcggca cgccgggttg
     2101 gactactggg ctctcggtca tgttcacgcc cgcgccgtcc tgagcacggg gcggcccctg
     2161 gccatctacc ccggcaacct gcaggcccgt gaccccggcg aggccggcac gaagggcttc
     2221 tacgtcgtcg aggtcgctga cggcgagccc accgcgcgct tcgtctcggc aggcgtcgtg
     2281 aacttcctga ccgtcgacgt ggacatcggg gccgacctcg cggacctcga cgccgtcaga
     2341 gaacgcctcg tccaagccgc gctcggcgca tccgaagacg cgtccgccgt atccggcacc
     2401 acggactcgg gcggggcgac ggtaggactc aaggcgcaac acgtcgtcat gcgggcccgc
     2461 gtgcgcggca tgggaccagc gcacgatgga ctgcgcaagg ccggggaggt cggtctcctc
     2521 ctgtacctgc aagacgtcgc gccggacggg gtctggtggg acgaggtcag gatctcgacc
     2581 aggccgccgc tcgaccgcgc ggcgcgcatg gcggcgggcg acttcgtggc cgacctgctc
     2641 gaggtgagca tggaacgcag gcatgaggcg cccgacaccg ttggtcgact cctcgacgag
     2701 ttgctaggga accgaagcct gcaggccgtg aggcaggaac tcggcttgga ccgcctgctg
     2761 gccgaggcgg acgacatctg ggacgaggcg gagacgctcg ccttcgacct gttgagcgac
     2821 gcgggaagcg gggcctgaag cgtgtacctc ggcagagtgg aagtgcacga gttccgcggg
     2881 ctcaaggacg tcgagttcga cctcgcaccc tccgccaagc ctggcctcgt cgtgctcgag
     2941 gggcccaacg aggccggcaa gagcacgctg ttggccttcg tgagggccat cctgttcgcg
     3001 gagcgcggcc aaggcgagtc gttgcacggg agcgtcacgc tccacgacgg ggcgaagagc
     3061 taccgcctgg agcgtaacgg agcccgctcg acctcgcggc tgctggatac ccagacgggc
     3121 acgcggctgg acctcgcgca gttggaccgt ctggtcggga acctcgacgg gaacgtctac
     3181 cgcaacgtgt tcgccttcgg gccctcggag ctgcagtcgt tcgacgcgct taccgacggg
     3241 gccgtcaggg aacgactcta ctcggccgcc gtggcaggcg cgggaaggaa cgccagcgct
     3301 gccacggccc acctcgaggc cgtgacgacc gagctgctca aaccgcgcgc ccagtcggta
     3361 ctcggcgacc tggcgcagga gctggccgag gcacgtaacg gcgccagccg agcccgctcg
     3421 gaagccaggc gctatgcgca gctcgccagc gagctggcag ccacgggctc cgaagccgac
     3481 tccctccgcc gcgaccagcg ggaggcgacg gctaccgcac tctggtacga gaagctgttg
     3541 cagctctggc cggactggag cggccgagcc gccgcgctgg acgacctgag acggctgagc
     3601 ctcgaggaag aggcgctcaa cgctcccggg gccggcggaa cgccgccgag cacgtggctc
     3661 cgcgccgccg gagctatcag ggacctcgcg gccgacctcg aggcgtatcg cagggccaag
     3721 gccgacgcgg cggaactcga agccgagcag cgggatctcg tcgaggcgag cctcaacggc
     3781 ctgcgtagcc ttgggccgga gtggggccca ccccgactgg cggcgttcgc cgacgccgga
     3841 gcttggaacg cgcggaccga ggtcgcccgg gccgccgccg ccgcggccgc cacggccctc
     3901 gacaaggtcg aggtcgcgct cgagaccgca cgacaagagc tgagcgcggc cgagcgcggc
     3961 ctcgctctgg cgacggccaa ggtagatacg tgggcgacag aagccggcag gcccgcccac
     4021 ggccccgcgg cggacccccg caaggccctg gagaccctcc tcgcgagggc gcgggaacgg
     4081 cgcctcggta tcgcgacgtt actcgaccgc ttcgacgacc tgaacgcggc gcgctcgcgt
     4141 catgcggacc tgctcgccaa actccagcag gtgggcgagc gcaccacggc tgcgctcgcc
     4201 agacgctggg tggcgttcgg actgactatc ctcgccgccg ccgccgtggc ggtcatcgag
     4261 tgggagggac gcccggcggt cgccggagtc gcggcggctc tgggcgttct gctcggcctc
     4321 ctgctctggc ctccgcgcgg gctgtcggcc gctgactccg cggtggcgga gctgcgcgcc
     4381 aacgccgccg aagccagcct cgacgtaggg cggtccgagg cggcgctagg cgatgcacgc
     4441 gcgcgcttgg ggctcgctcc cgacgcgggg gcgcccgagg ctcgcgccgc cgccagggag
     4501 gtcgaagac
//
LOCUS       DAOQYE010000044         4473 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_19827_length_4473_cov_2.939565, whole genome shotgun sequence.
ACCESSION   DAOQYE010000044 DAOQYE010000000
VERSION     DAOQYE010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 4473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4473
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19827_length_4473_cov_2.939565"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..993)
                     /locus_tag="PKN52_01465"
     CDS             complement(<1..993)
                     /locus_tag="PKN52_01465"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNQ98635.1"
                     /translation="MSPTATTRSHKHVLTPWYKQSAVGGPTFVRGEGSWLFDDQGKRY
                     LDLSAGLVSTNLGHGHPAVVKAIQEQAAKICYLPPSLIHDVRSEFAELLSEVSPWSEG
                     ARTFFTTGGGEANEDAIKMSRMITGRQKVLAAYRSFHGSAPGAGSLTGEDRRWANEPG
                     LPGIVHFFAPYPYRSPFFTEDPKLETARALEHLEQVITFEGGKNIAALVIEPVVGSNG
                     VIVYPDGYLAGLREITAKHGIVLVFDEVMTGFGRVGDRFAATRFGVTPDMITFAKGSS
                     SAYIPLGGVMVREGLAKHFDDVALMCGHTYAGHPMAMAAGLATVTTLKKEGLFER"
     gene            1314..2345
                     /locus_tag="PKN52_01470"
     CDS             1314..2345
                     /locus_tag="PKN52_01470"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNQ98636.1"
                     /translation="MKRPVLHALLISLALSGAGFAFAVWSVGGPKALLKVGELTPRYL
                     LYAAVALLASFVISALRLAFICRSLGYRLRLRHALRAHVLGLFSAAVTPGGSGNVAGI
                     ALTLDLQGAGSGRAWAAALATMSSDTTFHAWAMPLSLIALYSMGAYPRTPLWTVIGIV
                     TILLTMAFAYLLLFKVSLIGPIASRVLRGPLLRFRRRGLRLATSVIASSRTFASAPLW
                     QHVVIQLLTAAAWACYFTVLHFLLVGLHVHLGFALVLVGQTVVSVMSTFVPTPGASGF
                     LEVVLSWFLISKGSGEAGPATLFVWRLLTFYSLFILGPLLGGYMVVKMVGDGGKAKPT
                     AEGAEADPA"
     gene            complement(2421..3524)
                     /locus_tag="PKN52_01475"
     CDS             complement(2421..3524)
                     /locus_tag="PKN52_01475"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNQ98637.1"
                     /translation="MRVAILSKLSPGDGVVVYTRQLQAWLQGNGHAAHIVHFSNDPVD
                     SNRPADPDVHALPYLVRSPGYTLPGPGSYGRLKRLLQELGPDLIHAQLTTSPLDFALP
                     RLARELGVPVIGTFHHGWDYRPTRFGVFGQVVSNAYAPALAQYTRVIVFGSRLRRHFE
                     SRGVAAERIAVIPNGVDAAAYAPGPSEFRLEGKLRVTYMGRMTADKNIAPLVETFAAH
                     RHADLVLTLAGTGPLVASLKARFAADRRIEWLGFVADEAVRKSLWRGSDVVVLPSPIE
                     GLSLSLLEGMASGCMPLATDVGEHADVLSGLGRVLDPLNVRRDLRSAWDWLAANREAV
                     PALGAAARQRVLDRYTYERHWRALGELYADVLR"
     gene            complement(3547..3999)
                     /locus_tag="PKN52_01480"
     CDS             complement(3547..3999)
                     /locus_tag="PKN52_01480"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNQ98638.1"
                     /translation="MALNFETRVAAYGLIVDQGRLLLAHFSVPGLAPGWTLPGGGLEF
                     GEDPATAAVREVFEETGYHVELTGLLGTDSVHMRPEQRSRPGDLPLHSFRLIYAARLV
                     SGMLRSEADGSTDEARWFEFDEVTDLERVPLVDIGLRLWRERRGGHPR"
     gene            4128..>4473
                     /locus_tag="PKN52_01485"
     CDS             4128..>4473
                     /locus_tag="PKN52_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98639.1"
                     /translation="MRVGSTVVRAAYAVAVLAGLGCVAAAEPRQTQTLTLTPGEALPT
                     LWPGAVLVLEPGRYFGPWEIAVPDVTLRGNGATLTGASEGSSLVLSAPGIRVEGLTID
                     GSGPIDDLYTPDN"
BASE COUNT          604 a         1560 c         1608 g          701 t
ORIGIN      
        1 ccgctcgaac aggccttcct tcttgagggt ggtcaccgtg gcgaggcccg ccgccatggc
       61 catggggtgg ccggcgtacg tgtggccgca catgagggcg acgtcgtcga agtgcttggc
      121 gaggccctcg cggaccatca cgccgccgag cgggatgtac gcggaggacg agcccttggc
      181 gaacgtgatc atgtcgggcg tgacgccgaa gcgcgtggcg gcgaagcggt cgccgacgcg
      241 gccgaagccc gtcatgacct catcgaagac gagcacgatg ccgtgcttgg ccgtgatctc
      301 gcgcaggccg gccaggtagc catcggggta gacgatgacg ccgttcgagc cgaccacggg
      361 ctcgataacg agcgccgcga tgttcttgcc gccctcgaac gtgatgacct gctcgaggtg
      421 ctcgagggcg cgggccgtct cgagcttcgg gtcctccgtg aagaacgggc tgcggtaggg
      481 gtagggcgcg aagaagtgga cgatgccggg cagcccgggc tcgttcgccc agcgcctgtc
      541 ctcgccagtg aggctgccgg cgccgggggc ggagccgtgg aacgagcggt aggcggccag
      601 taccttctgg cggcccgtga tcatgcggga catcttgatg gcgtcctcgt tggcctcgcc
      661 gccgcccgtc gtgaagaacg tgcgggcgcc ctcgctccac gggctgactt cggagaggag
      721 ctcggcgaac tcggagcgca cgtcgtggat gagggagggc ggcaggtagc agatcttggc
      781 cgcctgctcc tggatggcct tgacgacggc cgggtgaccg tggccgaggt tcgtcgagac
      841 gaggccggcc gagaggtcga ggtagcgctt gccctggtcg tcgaagagcc aggagccctc
      901 gccacgcacg aacgtggggc cgcctaccgc cgactgcttg taccagggcg tgagcacatg
      961 cttgtgtgaa cgagtggtgg ctgtagggga catcgcctga cctcctagaa tcgtccccct
     1021 ccggcgctgg gcgcgcgtga aggggcctcc cagacgttgc cgccgggagc cgccggatac
     1081 cggtgttggt cagcaggtta ccgcatcttg cgcggccgcg gtgggaaaaa gctgtgcttc
     1141 gcgcgaggtg aaggctgtgc ttcaagcggg gtggaggccg tgctgcccgg cggcccgccg
     1201 tggtgcctgc cgggcggccg cgcgacacga ccctgtatgc tgaaccgccc agtgtgcgac
     1261 ggcacgaact gaccgatggt cctcggcacg ccactggtcc tcggcacgcc accatgaagc
     1321 gacccgtact ccacgccctg ctgatctccc tcgccctgtc cggcgcgggc ttcgccttcg
     1381 ccgtctggag cgtcggcgga ccgaaggccc tcctgaaggt cggcgagctg accccgcgct
     1441 acctgctgta cgcggccgtg gcgctgctcg cgtcgttcgt catctcggcg ctgcgcctcg
     1501 ccttcatctg ccggtccctc ggctaccggc tcaggttgcg gcacgccttg cgggcccatg
     1561 tcctcggcct gttctccgcc gccgtgacgc cgggcggcag cgggaacgtg gccggcatcg
     1621 cgctcaccct cgacctgcag ggcgctggca gcggtcgcgc ctgggcggcg gcgctcgcca
     1681 ccatgtcgtc ggacaccacg ttccacgcgt gggccatgcc cctgtccctc atagccctct
     1741 actcgatggg cgcctacccg cgcaccccgc tctggacggt catcggcatc gtcacgatcc
     1801 tcctgaccat ggcgttcgcg tacctgctcc tcttcaaggt gagcctgatc ggcccgatcg
     1861 cgagccgggt cctgcgcggc ccgctcctgc ggttcaggcg ccgagggctg cgcctggcca
     1921 ccagcgtgat cgccagcagc cggacgttcg cctccgcccc gctctggcag cacgtcgtca
     1981 tccagctcct caccgcggcg gcgtgggcct gctacttcac cgtcctgcac ttcctgctgg
     2041 tcggcctgca cgtgcacctc ggcttcgcgc tcgtgctggt cggtcagacg gtcgtgtcgg
     2101 tcatgagcac cttcgtgccc accccgggcg ccagcggctt cctcgaggtc gtgctgagct
     2161 ggttcctcat cagcaagggg agcggcgagg ccggtcctgc caccttgttc gtgtggcggc
     2221 tgctgacgtt ctacagcctc ttcatcctcg ggccgctcct gggcggctac atggtcgtca
     2281 agatggtcgg tgacggcggc aaggccaagc cgacggcgga gggcgcggag gcggacccgg
     2341 cctaggccga ccccggttgg ggcatgctaa cgaggccggc gcaggcaggg tgcgttggcg
     2401 gcgctgagcc gaagctgaag ctagcgcagc acgtcggcgt agagctcgcc gagcgcccgc
     2461 cagtgccgct cgtacgtgta gcggtccagc acccgctggc gggcggcggc gccaagcgcc
     2521 ggcacggcct cgcggttggc cgccagccag tcccaagcgg agcgcaggtc ccggcgcacg
     2581 ttgagcgggt cgagcacgcg cccgaggccg ctcagcacgt cggcgtgctc gcccacgtcc
     2641 gtcgccaggg gcatgcagcc gctcgccatg ccctccagca ggctcaggct caggccctcg
     2701 atggggctcg gcagcacgac gacgtccgag ccccgccaga ggctcttgcg caccgcctcg
     2761 tcggccacga agccgagcca ctcgatgcgg cggtcggccg cgaacctggc cttcaggctc
     2821 gccacgagcg ggccggtccc cgccagggtc agcaccaggt cggcgtgccg gtgggcggcg
     2881 aaggtctcga cgagcggcgc gatgttcttg tcggccgtca tgcgccccat gtacgtgacg
     2941 cgcagcttgc cctcgagccg gaactcgctc ggccccggcg cgtaggcggc ggcgtcgacc
     3001 ccgttgggga tgacggcgat gcgctctgcc gccacgccgc gcgactcgaa gtgccgccgc
     3061 aggcggctgc cgaagacgat cacgcgcgtg tattgcgcca gcgccggcgc gtacgcgttg
     3121 ctcacgacct ggccgaagac gccgaacctg gtcggtcggt agtcccagcc gtggtggaag
     3181 gtcccgatga cgggcacgcc cagctcgcgc gccaggcgcg ggagcgcgaa gtccagcggg
     3241 ctcgtcgtca gctgcgcgtg gatgaggtcc ggtccgagct cctgcaagag ccgcttcagc
     3301 cgcccgtacg agccggggcc gggcagcgtg tagccgggcg accggaccag gtaggggagg
     3361 gcgtggacgt cagggtcggc cgggcggttc gagtcgaccg gatcgttcga gaaatggacg
     3421 atgtgggcgg catggccgtt gccttgcagc cacgcctgca gttgccgcgt gtagacgacg
     3481 accccgtcgc cggggctcag cttgctgagg atggctacgc gcatgcgccc ctgccccccg
     3541 gcacgctcac cgagggtggc ctccgcgccg ctcgcgccag agccgcagcc cgatgtccac
     3601 cagtggcacg cgctccaggt cggtgacctc gtcgaactcg aaccagcgcg cctcgtcggt
     3661 cgagccgtcc gcctccgacc tgagcatgcc cgagacgagc cgtgccgcgt agatgaggcg
     3721 gaaggagtgg agcggcaggt cgccgggtcg gctacgctgc tcagggcgca tgtgcacgct
     3781 gtcggtcccg aggaggccgg tcagctcgac gtggtagccc gtctcctcga acacttcccg
     3841 cacggccgcc gtcgccgggt cctcgccgaa ctccaggccg ccgcccggca acgtccagcc
     3901 cggcgcgagg ccgggcacgc tgaagtgcgc cagcaggagc cgcccctggt cgacgatcaa
     3961 cccgtaggcg gccacgcgcg tctcgaagtt aagtgccatc gcaacgagct tagcccgtgt
     4021 cgtcgtggtc gtagcggacg gcggcgcctc ttcgcgccta cgccacgtgg gtaccggtgc
     4081 gcgctcgtcg ggacattgtt ctgccggctt gcggttacgc tcatggaatg agagtcgggt
     4141 cgaccgtcgt gcgcgctgcg tacgcggtag cggtgttggc tggtcttggc tgcgtagccg
     4201 ccgccgaacc gcgacagacc caaacgctga ctctgacacc tggtgaggcg ctgccgacgc
     4261 tgtggccggg agcggtgttg gtgctcgagc ccggccgcta ctttggtccc tgggagatcg
     4321 cagtgcccga cgtgacgttg cgcggcaacg gggccacgct gacaggcgcc tcggagggca
     4381 gcagcctggt cctgtcagcc cccggcatcc gggtcgaggg gctcacgatc gatggctccg
     4441 ggccgatcga cgacctgtac acgcccgaca acc
//
LOCUS       DAOQYE010000045         4464 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_19894_length_4464_cov_3.700612, whole genome shotgun sequence.
ACCESSION   DAOQYE010000045 DAOQYE010000000
VERSION     DAOQYE010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 4464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4464
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19894_length_4464_cov_3.700612"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1108
                     /locus_tag="PKN52_01490"
     CDS             <1..1108
                     /locus_tag="PKN52_01490"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:NF025001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HNQ98640.1"
                     /translation="ANSISALERSVTQLDSEIAAAVASGDAAEAQRLQALGATLREQR
                     VRELEAARARSASAVIVGLLENLSVATPPIMASGPRLVFNVFLAVLLGLVLGYGVQFF
                     IWSLSTSVKDREELSSLLGTQVLGELKRVGRNGSRFSPDQVGFFRASLLSVLRNVRPR
                     VIGISSPEDYADKQGVAASLAESLWRSGLRTLLIDADLRRKGPGLGIDVSKTDTPGLE
                     TYLQDQSAPLQTVSITADSRIAFDIIPVRTLSKQPSELLAYGFEAFMKRVDAAYDVIV
                     IDLPPVLSYPDALAAAPACSSGIVLAVSVGSRGKQAANAAALLEMNEVELRGAVLTGG
                     SLNREARPRVSIGRRDGVAAPPARVSARVTQRQK"
     gene            1124..2164
                     /locus_tag="PKN52_01495"
     CDS             1124..2164
                     /locus_tag="PKN52_01495"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNQ98641.1"
                     /translation="MRKGALVISLDFELMWGIRDTLAAGGGYRDNLYGAREAVPRILD
                     LFTEYDVAATWATVGFLFAESKEELELCSPSVKPRYANSALDPYAERLGENERDDPLH
                     FAPSLVKEIATRPRQEVGSHSFSHYYCLEPGQGLAEFEADVGSAVRMAAARGLVLRSF
                     VFPRNQVRPDYVGVLASHGFTSYRGAEANLLNRPRPGASGSTPIRAVRLADTFLNLTG
                     SGAVPWTDLMRGGELVNVRGSRFLRPWTANVVFESMRWNRVANSMVAAARRGDLYHLW
                     WHPHNFGVNIVENLAALRRHLELFRKLSGEYGFRSLSMSEAAEAARLRCATANDHPKD
                     LHTVHHAGQASS"
     gene            complement(2106..3278)
                     /locus_tag="PKN52_01500"
     CDS             complement(2106..3278)
                     /locus_tag="PKN52_01500"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNQ98642.1"
                     /translation="MGSLKPSDAPGRSLLITTSVPSTVTAFLLPYVEHFRRLGWRVEA
                     ASNGITAAPKCAAAFDAVHDVPWTRKPTDLVNVTTAADTLRRIVREGRFDIVHTHDPV
                     AAFITRFALRHERRADRPKVVYTAHGFHFYRGAPLVQGTVFKVVERLASGWTDHLIVI
                     NQEDLASAHRFPLARDGKVSLMPGIGIDCSVYSLEKVSSAEVERVRRDLCLVSGQPLF
                     TMIAEFNPGKRHRDAVAALAASGLQTAVLALAGEGPLMQEVRALADAAGVGDRVRLLG
                     YRNDIPALVAASAAVLLPSEREGLPRSLMEASALERPVIASRIRGVSELVTERTGFLH
                     EVGDVGAIAAALRAVVNDPAGMAEMGRAGRKAMERYDIKNLLALHDALYAGLLGDR"
     gene            complement(3266..4432)
                     /locus_tag="PKN52_01505"
     CDS             complement(3266..4432)
                     /locus_tag="PKN52_01505"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNQ98643.1"
                     /translation="MNIQFVTTSLMQGGAELQVYLLATQLKRRGHEVSVVSMRDPEAF
                     VDGLQELGIPFHSLAMERGSADVKAIAKLGNLVRELKPDVVHSHMVHANLLARLTRLF
                     APFPVQVSTAHNLTEGARWRDYAYRLTDPLCDLTTNVCGKCVERFIQVGATPANKIRY
                     VPNGLDFSTFKPTPGSHAAKRRELGVDDTTFLWLAVGRLEEQKDYPTMFEAVARLVAS
                     FSSAHGPWRGMKFVIAGSGSLEERLKTSVHEAGLDPHVAFLGDRKDVADLMGAADGYV
                     MSSAWEGLPMVLLEAGAARLPAVVTDVGGNREAVTGEAVGALVPSADAAALASAMTRL
                     MSLGKDELEQMGQRFRDHVEGTFGITRVVELWEAVYHELLTRARDGARPRRWAA"
BASE COUNT          755 a         1419 c         1485 g          805 t
ORIGIN      
        1 cgccaacagc atctccgccc tcgagaggtc ggtgacccaa ctcgacagtg agatcgccgc
       61 tgccgtcgca tccggcgacg ccgccgaggc tcaacgtctg caggcactcg gcgccaccct
      121 gcgtgagcag cgggtgcgcg aactggaggc cgcgcgtgcg cgcagcgcct cggcggtgat
      181 agttggcctg ctcgagaacc tgagcgtggc cacgccaccg atcatggcca gtgggcccag
      241 gctcgtgttc aacgtcttcc tggccgtcct gctcggtctc gtccttggtt acggcgtcca
      301 gttcttcatc tggtctctca gcacctcggt caaggaccgg gaggagctgt cctcgctcct
      361 cggcacgcag gtgctcggcg agctcaagcg cgtggggcgc aacggcagca ggttctcgcc
      421 cgaccaggtc ggtttcttca gggcgagcct actgagcgtc ttgcgcaacg tccgtcctag
      481 ggtaatcggt atctcgagtc cggaagacta cgccgacaag cagggggtag ctgcctcctt
      541 ggccgagagc ctctggagaa gtggccttcg cactctcctc atcgacgccg accttaggcg
      601 caaagggccg ggcctcggca tcgacgtctc gaagacggac acccctggcc tggagactta
      661 cctccaggac cagagcgcac cgttgcagac ggtgtctata actgccgact ctaggatcgc
      721 tttcgacata atcccggtca ggacgctctc caaacagccc agcgagttgt tggcctatgg
      781 gttcgaggcc ttcatgaagc gggtggatgc cgcgtatgat gtgatcgtca tagatcttcc
      841 accggtgctc tcatatccgg acgctctggc tgcggccccg gcttgtagca gcggtatcgt
      901 cttggcggtg tccgtaggga gcaggggcaa gcaagcggcc aacgcggcgg ctttgctgga
      961 gatgaacgag gtcgagcttc gcggtgcagt actgaccggg ggctcgctga acagggaggc
     1021 taggcctagg gtctcgattg gtaggcgaga tggcgtggcc gctccgccgg caagggtctc
     1081 cgccagagtg acccagaggc agaagtgagg ggtggagggg gctgtgcgaa agggtgcgct
     1141 tgtaatatcg ctcgatttcg agttgatgtg ggggatacga gacaccctgg ctgcaggagg
     1201 gggttacagg gataacctct acggcgctcg cgaggcggtg cctcgcatcc tggatctctt
     1261 cactgagtac gacgttgccg ccacgtgggc cactgtgggg ttcctcttcg cggaatcgaa
     1321 ggaggaacta gagctctgct caccgagcgt gaagcctagg tacgcgaact cggcgctgga
     1381 tccttacgcc gagcggttgg gagagaacga gcgggacgac cccctccact tcgcgccgtc
     1441 gctagtgaag gagatcgcga ctaggccgcg gcaagaggtc ggaagccata gtttctcgca
     1501 ctactactgc ctggagcccg ggcagggctt ggcggagttc gaggcggatg tcgggagcgc
     1561 ggtcaggatg gcggctgcgc ggggcctcgt cctacgttcg ttcgtcttcc cacgcaacca
     1621 ggtgcgacca gattacgtag gggtgctcgc ttcgcatggc ttcacctcct acagaggggc
     1681 cgaggccaac ctactcaacc gtccgaggcc gggagcttca gggtcgaccc ccatcagggc
     1741 agtgaggctc gcggacacct tccttaacct gacgggctcc ggagcggtcc cctggaccga
     1801 cctcatgcgc ggaggcgagc tggtcaacgt gcgggggagc cggttcctcc ggccctggac
     1861 ggctaacgtg gtgttcgaga gcatgcgatg gaaccgcgtg gctaattcaa tggtggcggc
     1921 cgcgcggcgt ggcgacctgt accacctctg gtggcacccg cacaacttcg gggtgaacat
     1981 cgtcgagaac ctggccgcgt tgcgccgtca tcttgagctt ttcaggaagc tgagcggaga
     2041 gtacggcttc cgtagcctgt ccatgtcgga ggcagcggag gcggcgcgcc tgcggtgcgc
     2101 gacggctaac gatcacccaa aagacctgca tacagtgcat catgcagggc aagcaagttc
     2161 ttgatgtcgt agcgctccat cgctttgcgc ccggcgcgcc ccatctcggc catgcctgcg
     2221 gggtcgttca ctacggccct cagcgctgca gcgatggcac caacgtcccc aacctcgtgc
     2281 aagaagcccg tccgttcagt gacgagttcc gagacgcccc ggatgcggct ggctatcacg
     2341 ggcctctcta gcgccgaggc ctccatcagc gagcgtggca ggccctcgcg ttccgagggc
     2401 agcaacaccg ccgccgaggc cgccaccagg gcggggatat cgttacggta accgagtagt
     2461 ctcacgcggt cgcccacccc cgcggcgtct gcgagagcgc gtacttcctg catgagcgga
     2521 ccctcgcccg cgagcgccaa tacggcagtc tgtagaccgc tcgcggctag cgcggccacc
     2581 gcatcacggt ggcgcttgcc ggggttgaac tccgcgatca tggtgaatag aggctgtccc
     2641 gataccaggc agaggtcacg ccggacgcgc tcgacttccg cgctgctcac cttctccaga
     2701 ctgtagacag agcagtcgat gcctatcccg ggcatgaggc tgaccttccc atccctggct
     2761 agcgggaagc ggtgggcaga ggcgaggtcc tcttggttga tgacgatgag atggtcggtc
     2821 cagccgcttg ccagccgctc gacgaccttg aagacggtgc cttgcaccaa gggtgctcca
     2881 cggtagaagt ggaagccgtg cgccgtgtag acgaccttcg gtcggtcggc acgccgctcg
     2941 tggcgcagag cgaagcgcgt tatgaacgcc gctacggggt cgtgcgtgtg gactatgtcg
     3001 aaacgacctt cccgcacgat gcgtctaagc gtgtcggccg ccgtcgtgac gttgacgagg
     3061 tcggtcggtt tcctcgtcca ggggacatcg tgaacggcgt cgaacgccgc cgcgcacttg
     3121 ggcgccgcgg ttatgccgtt gctggccgct tcgacccgcc agcccaaccg cctgaagtgc
     3181 tccacatagg gcaggaggaa ggcggtcacc gtcgagggca cgctggtggt gatgaggaga
     3241 ctgcgccccg gcgcgtcgga aggcttcagg ctgcccaccg ccttggcctg gcaccatcgc
     3301 gcgctctggt caggagttcg tgatagacag cttcccacag ctcgaccacg cgagtgatgc
     3361 cgaaagttcc ctcgacgtga tccctgaagc gctgtcccat ctgctccagc tcgtccttgc
     3421 ctaatgacat caggcgggtc atcgcggacg ccagcgccgc agcgtcggca gacggcacga
     3481 gagcgcctac cgcctcgccc gtgacggcct cccggttccc accgacgtcc gtgaccactg
     3541 ccgggagccg cgcagccccc gcctcgagga ggaccatcgg cagcccttcc caggccgacg
     3601 acatcacgta gccgtcggcg gcgcccatca gatcggcgac gtccttgcgg tctccaagga
     3661 aggcgacatg cgggtcgaga ccggcttcgt gcacggacgt cttgagccgc tcttcgaggg
     3721 agccgcttcc ggcgatgacg aacttcatgc cccgccacgg cccgtgcgcg ctactgaaag
     3781 aggcgaccag tcgagccacc gcctcgaaca tggttgggta atccttctgc tcttccaggc
     3841 ggcccacggc gagccatagg aaggtggtgt cgtcaacgcc gagctcgcgc cgtttggcag
     3901 cgtggctgcc tggcgtcggc ttgaaggttg agaagtccag gccgttcggc acgtacctga
     3961 tcttgttggc gggggtggcg ccgacctgga tgaaacgctc cacgcacttg ccgcagacgt
     4021 tggtggtgag atcgcacaac gggtcggtca gcctgtacgc gtagtcgcgc caccgggcgc
     4081 cctctgtgag gttatgcgcc gttgacacct ggacagggaa cggtgcgaac aggcgggtaa
     4141 ggcgtgccaa caggttcgcg tggaccatgt ggctatgcac gacgtcgggt ttgagttccc
     4201 tgacgaggtt gccgagcttg gcgatggcct tgacgtccgc cgagccccgc tccatggcca
     4261 ggctatggaa ggggatgccg agctcctgca ggccgtcgac gaaggcctca gggtctcgca
     4321 tgctcacgac ggagacctcg tgcccccgcc gcttgagctg cgtggccaag agatagacct
     4381 gcaactctgc tccgccttgc ataagcgaag tggtcacgaa ctggatgttc atcgggctgg
     4441 ttccttggcc catcgtctcc tacc
//
LOCUS       DAOQYE010000046         4443 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_20037_length_4443_cov_3.059025, whole genome shotgun sequence.
ACCESSION   DAOQYE010000046 DAOQYE010000000
VERSION     DAOQYE010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 4443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4443
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20037_length_4443_cov_3.059025"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="PKN52_01510"
     CDS             complement(<1..365)
                     /locus_tag="PKN52_01510"
                     /inference="COORDINATES: protein motif:HMM:NF012407.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HNQ98644.1"
                     /translation="MSYRIAVLPGDFIGPEVIDATLEVLEALAPRHGLAFEFERLPFG
                     GNAIESHGEPLPQVTRDAAARSDAVLMGSAGGPVGDHPWNRLPREKRVESGILALRKH
                     LQVFANLRPVRVFPGLERLS"
     gene            complement(362..979)
                     /gene="leuD"
                     /locus_tag="PKN52_01515"
     CDS             complement(362..979)
                     /gene="leuD"
                     /locus_tag="PKN52_01515"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HNQ98645.1"
                     /translation="MALTPVTEARGTAVPIMANDIDTDRIIPARYLKAVTFETMGEGL
                     FYDERFDADGRSKGHPLDDPHRAGAAIIVTGDNFGCGSSREHAPQSIMRAGFKGMIAG
                     SFAEIFFGNCTTLGLVCAVVDEAGRNEIAALVERDPSAEVVIDVTAQEVRAGGKTYRA
                     SIRDTAREALLTGYWDPIGELLEGVEQVKALAAARGYPVSVGEAR"
     gene            complement(981..2408)
                     /gene="leuC"
                     /locus_tag="PKN52_01520"
     CDS             complement(981..2408)
                     /gene="leuC"
                     /locus_tag="PKN52_01520"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HNQ98646.1"
                     /translation="MNEPRSLFDKVWDSHTVGRLRNGQDQLFIDTHLIHEVTSPQAFG
                     MLEDLGVDVLLPERTFATVDHIVPTHTRVEPFADPMAAAMIAELRKNTAKHGITFFDV
                     GGGRQGIVHMVGPEQGITQPGSTIACGDSHTSTHGAFGAIAFGIGTSQVRDVLATQTM
                     AMSKPKLRRIEVNGRLRPGVYAKDVVLHIIRTLGVKGGIGYAYEYAGTTFDAMTMEER
                     MTVCNMSIEGGARCGYVNPDATTFAYLDGRPYSPKGAAWDAAVASWKALASDPGCRYD
                     DVVVIDAVDVPPTVTWGINPGQAIAIDELVPTVEGAPEHDKAGVAEALAHMKLQGGAP
                     IKGTKVDVAFFGSCTNARITDFIEVAKVMKGRKVHPGVQAIAVPGSHLVKLECEKLGI
                     DAVLKEAGFEWREAGCSMCLAMNPDKLVGDQLCASSSNRNFKGRQGSPTGRTVLMSPV
                     MVAAAAVTGVVSDAREVFGAGKAVA"
     gene            complement(2658..3851)
                     /locus_tag="PKN52_01525"
     CDS             complement(2658..3851)
                     /locus_tag="PKN52_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HNQ98647.1"
                     /translation="MADQIRSTQIQADIVDLLKLAIERRASDLLLTVGLPPMLRLDGE
                     WRPTEYEVLNGTMTRRLMYALMDEKKQRNFEERKDLDFSFSLTGHGRFRVNVFMQRGS
                     VGGVMRTISEEVLTFADLGLPQEAADIARLPRGLVLVTGPTGSGKSTTLATMLDLVND
                     EYSKHIVTIEDPIEFFHRHKTSIVNQREVGEDTANFQAALRSVLRQAPDVILVGEMRD
                     FETIGAAVTAAETGHLVLATLHTNSAAEAIDRIIDVFPDAQQAQVRVQLANNLQAILT
                     QQLIPRASGDGRVLAYELLIATPGVRNLIREGKTHQIPAQIQMGGAIGMITMDACLAE
                     LHLRKAISFEIGSSRAVDAKEFERLVSTGGQAQGASAPDQGRPTPPTPTTPRRGTPPL
                     GRGAR"
     gene            complement(3878..>4443)
                     /locus_tag="PKN52_01530"
     CDS             complement(3878..>4443)
                     /locus_tag="PKN52_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011173991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNQ98648.1"
                     /translation="LRSFLRQDPDIIMVGEIRDHETAKISMEAALTGHLLIATLHTND
                     SAGAITRLQEMGIEPFNVSASLIGVLAQRLVRRVCKECKVASSPDPDVLRRLGISEKD
                     MAGKSLYRGVGCEKCGGSGYNGRYAIHELLVVDEELEKAIVQEASSIELRDLAVKRGM
                     KTLRDDGINKAFQGITTLEEVLARTAE"
BASE COUNT          609 a         1462 c         1620 g          752 t
ORIGIN      
        1 gagaggcgct cgaggccggg gaagacgcgg acggggcgca ggttggcgaa gacctgtagg
       61 tgcttgcgca gcgcgaggat gccggactcg acgcgcttct cgcgcggcag cctgttccag
      121 gggtggtcac cgaccggacc gccggccgaa cccatgagga ccgcgtcgga gcgtgccgcc
      181 gcgtcgcgcg tgacctgtgg cagcggctca ccgtggctct cgatcgcgtt gccgccgaac
      241 ggcaggcgct cgaactcgaa cgcgaggcca tgacggggcg cgagcgcctc gaggacctcc
      301 agcgtcgcgt cgatgacctc tggcccgatg aagtcgcccg ggagcaccgc gatgcggtag
      361 ctcatcgcgc ctcaccaacg ctcacggggt agccgcgggc ggcggccagc gccttcacct
      421 gctcgacgcc ctccagtagc tccccgatcg ggtcccagta gccggtgagg agcgcctcgc
      481 gcgccgtgtc gcggatgctg gcgcggtagg tcttgccgcc ggcgcgcact tcctgcgccg
      541 tgacgtcgat gacgacctcg gcgctcgggt cgcgctcgac cagcgcggcg atctcgttgc
      601 gcccggcctc gtcgaccacg gcgcagacca gcccgagcgt cgtgcagttg ccgaagaaga
      661 tctcggcgaa cgagccggcg atcatcccct tgaagcccgc gcgcatgatc gactgcggtg
      721 cgtgctcccg gcttgagccg cagccgaagt tgtcgcccgt cacgatgatg gcggcgcccg
      781 cgcggtgcgg atcgtcgagc gggtgcccct tcgagcgccc gtcggcgtcg aagcgctcgt
      841 cgtagaagag cccctcgccc atggtctcga acgtgacggc cttgaggtag cgcgccggga
      901 tgatgcggtc ggtgtcgatg tcgttggcca tgatgggcac cgcggtgccg cgggcctccg
      961 ttacgggggt gagcgccatg tcaggccacc gccttcccgg cgccgaacac ctcgcgggcg
     1021 tcggacacca cgcccgtcac ggccgcggcg gcgaccatga cggggctcat gagcacggtg
     1081 cgcccggtgg gggagccctg gcgccccttg aagttgcggt tggaggacga ggcgcagagc
     1141 tggtcgccca cgagcttgtc ggggttcatg gcgaggcaca tgctgcaacc cgcctcgcgc
     1201 cactcgaagc cggcctcctt gagcacggcg tcgatgccga gcttctcgca ctccagcttg
     1261 acgaggtgcg agccgggcac cgcgatggcc tggacgccgg ggtgcacctt gcggcccttc
     1321 atgaccttgg cgacctcgat gaagtcggtg atgcgggcgt tcgtgcacga gccgaagaag
     1381 gcgacgtcga ccttggtgcc cttgatgggg gcgcccccct ggagcttcat gtgggcgagg
     1441 gcctccgcca cgccggcctt gtcgtgctcc ggcgcccctt ccacggtcgg gacgagctcg
     1501 tcgatggcga tggcctggcc ggggttgatc ccccaggtga ctgtcggcgg cacgtccacc
     1561 gcgtcgatga ccaccacgtc gtcgtagcgg cagcccggat cggaggcgag cgccttccag
     1621 ctcgcgacgg cggcatccca agccgcgccc tttggcgagt agggccggcc gtcgaggtag
     1681 gcgaacgtgg tcgcgtcggg gttgacgtag ccgcagcgcg cgccgccctc gatggacatg
     1741 ttgcacaccg tcatgcgctc ttccatcgtc atggcgtcga acgtcgtgcc ggcgtactcg
     1801 tacgcgtagc cgatgccgcc cttcacgccg agggtgcgga tgatgtggag gacgacgtcc
     1861 ttggcgtaga ccccggggcg cagccggccg ttcacctcga tgcgccgcag cttcggcttg
     1921 ctcatggcca tcgtctgggt ggcgagcacg tcgcgcacct gggaggtgcc gatgccgaac
     1981 gcgatcgcgc cgaacgcgcc gtgcgtgctc gtgtgcgagt cgccgcaggc gatcgtcgag
     2041 cccggctggg tgatgccctg ttcgggcccg accatgtgga cgatcccttg ccggccgccg
     2101 ccgacgtcga agaacgtgat gccgtgcttg gccgtgttct tgcgcagctc cgcgatcatc
     2161 gcggccgcca tcgggtcggc gaacggctcc acgcgcgtgt gcgtcggcac gatgtgatcg
     2221 accgtcgcga acgtgcgctc cggcagcagg acgtccacac cgaggtcctc cagcatgccg
     2281 aaggcttgcg ggctcgtgac ctcgtggatg aggtgcgtgt ctatgaagag ctggtcctgg
     2341 ccgttgcgca ggcgcccgac cgtgtgggag tcccagacct tgtcgaagag gcttctaggt
     2401 tcgttcatgc tgtctcgttc cttcgcgccc cgctcacgtg cgaagacccc cggccgcggg
     2461 tgggccgggg gtcgtgatgc gctgtcgctt gtaggaccgt gcccggccca ccgaagctgc
     2521 ggctcacgcc tcccgctcgg ctagccacaa ggccgctcgc cactggacga aggcgaggcg
     2581 ccgtctggtg cgcgagacct tgccggtggc tgagttggcc gtcatgcggc gaagtataag
     2641 agcgccggat gcggccgtca acgagcgcct cgcccgagcg gcggggtgcc gcgccgcggc
     2701 gtcgtcggcg tgggtggggt cggacgaccc tgatcgggtg cggaggcgcc ttgggcctga
     2761 ccgcccgtcg agacgaggcg ctcgaactcc ttggcgtcga cggcgcgtga ggagccgatc
     2821 tcgaaggaga tggccttgcg caagtgcagc tcggcgaggc aggcgtccat ggtgatcatg
     2881 ccgatggcgc cccccatctg gatctgggcg gggatctggt gcgtcttgcc ttcgcggatc
     2941 aggttgcgca cgccgggggt ggcgatgagc agctcgtagg cgaggacgcg gccgtcgcca
     3001 ctggcgcgcg ggatgagctg ctgcgtcagg atggcctgca ggttgttggc gagctgtacg
     3061 cgcacctgcg cctgctgggc gtcggggaag acgtcgatga tgcggtcgat cgcctcggcg
     3121 gcgctgttgg tgtgcagggt ggcgagcacg aggtggccgg tctcggcggc ggtcacggcc
     3181 gcgccgatcg tctcgaagtc gcgcatctcg cctaccagga tcacgtcggg ggcctgcctc
     3241 agcacgctgc gcaaagccgc ctggaagttg gccgtgtcct cgccgacctc ccgctggttg
     3301 acgatcgacg tcttgtgccg gtggaagaac tctatcgggt cctcgatggt gacgatgtgc
     3361 ttcgagtact cgtcgttgac gaggtcgagc atcgtcgcga gcgtggtgga cttgccggag
     3421 cccgtcgggc ccgtcacgag cacgaggccg cggggcaggc gcgcgatgtc ggccgcctcc
     3481 tgcggcaggc caaggtcggc gaaggtgagg acctcctccg agatcgtgcg catgacgccg
     3541 ccgacggagc cgcgctgcat gaagacgttg acgcggaacc gcccgtggcc ggtgaggctg
     3601 aacgagaaat cgaggtcctt gcgctcttcg aagttgcgct gcttcttctc gtccatcagc
     3661 gcgtacatga ggcgccgcgt catcgtcccg ttcaggacct cgtactcggt cgggcgccac
     3721 tcgccgtcga ggcgcagcat gggcgggagg ccgacggtga ggagcaggtc ggaagcccgc
     3781 ctctcgatgg ctagtttgag caggtcgacg atgtctgcct ggatctgggt cgagcggatc
     3841 tggtctgcca tgcgggaaag tcctctcggg tcgcgcgcta ctcggccgtg cgcgcgagca
     3901 cctcttccag ggtcgttatg ccctggaagg ccttgttgat gccgtcgtcg cgcagagtct
     3961 tcatgccgcg cttcaccgcc aggtcgcgca gctcgatgga ggacgcctct tggacgatgg
     4021 ccttctcgag ctcctcgtcc acgacgagca gctcgtggat ggcgtaacgg ccgttgtaac
     4081 cgctgccacc gcacttctcg cacccgacgc cgcggtagag cgacttgcct gccatgtcct
     4141 tctcggagat gccgaggcgg cgcagcacgt ccgggtccgg gctcgaggcg accttgcact
     4201 ccttgcagac gcgcctgacc aggcgttggg ccagcacgcc gatgagcgac gcggagacgt
     4261 tgaagggctc gatgcccatc tcctgcaggc gcgtgatggc gccggcggag tcgttggtgt
     4321 gcagcgtggc gatgagcagg tgacccgtga gggcggcctc catcgagatc ttggccgtct
     4381 cgtggtcgcg gatctcgccg accatgatga tgtccgggtc ctggcgcagg aacgagcgca
     4441 gcg
//
LOCUS       DAOQYE010000047         4443 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_20039_length_4443_cov_2.775980, whole genome shotgun sequence.
ACCESSION   DAOQYE010000047 DAOQYE010000000
VERSION     DAOQYE010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 4443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4443
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20039_length_4443_cov_2.775980"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1140)
                     /locus_tag="PKN52_01535"
     CDS             complement(<1..1140)
                     /locus_tag="PKN52_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98649.1"
                     /translation="MALRRVSVASLLTVLTVSGLFGCGGPGPVRPQDDFVVEGKVRLP
                     QGSPLDPQELRVLGANGSSDLAADGGFAVEVNEDGLNLLAVVGQDGRLVLLGFLGTRE
                     AEAELSGRSTAAALLYMALGGQFQEAWLQERMFEALGVEPEVAALDAELSSILAADPG
                     ALADGSDALVNAVMAARERLAARGRSGAGAFVTAQTSDADPTGMGGSGRSFPADLLAA
                     AATDKNYVVITDPHEEAGMETLLADSGTGVYLKNSKRRPARLFAYETGVKVGDGPVVQ
                     VGPVPVGSYLSVPPSNSLEFGATLGAIASGDFMSTPLAPAYSEPAELPLENGTTRTYY
                     TVIALAPSLSAELPAVFTDSRFFGFRSEWDRQLLELDFEVFWSNLL"
     gene            complement(1209..1538)
                     /locus_tag="PKN52_01540"
     CDS             complement(1209..1538)
                     /locus_tag="PKN52_01540"
                     /inference="COORDINATES: protein motif:HMM:NF016854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HNQ98650.1"
                     /translation="MDADSERLAHELLGWLEPLGGRIRAMFGGYCFYLDGKPVGLVND
                     GDIFVKRSSAEAKFAGLAELGPAYPGAKESWRLTPDALQAEPLRVVEAIHATAAALPA
                     RRPARRR"
     gene            complement(1585..2160)
                     /locus_tag="PKN52_01545"
     CDS             complement(1585..2160)
                     /locus_tag="PKN52_01545"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HNQ98651.1"
                     /translation="MAELVLFHHVLGLTPGIVALADELRAGGHTVHTPDMYEGKTFAT
                     LDEGMAHVRSVGFAAVTARGVAAVAGLGPGLVYAGYSLGVMPAQQLAQTRAGARGALL
                     IAACMPTEEFGGPWPAGVPLQIHAQEHDPEFDNGYDLPTAQAVVLEAADGELFLYPGD
                     KHFFADSSTPEYDAGATSEFVRHALAFLARV"
     gene            complement(2182..>4443)
                     /locus_tag="PKN52_01550"
     CDS             complement(2182..>4443)
                     /locus_tag="PKN52_01550"
                     /inference="COORDINATES: protein motif:HMM:NF013124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043531 - ADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NB-ARC domain-containing protein"
                     /protein_id="HNQ98652.1"
                     /translation="GEELLTWVTAVREALVERMQRSAVAEARRLAAAGRRAQAGALAD
                     AAYAAAGQGGSDPACLEALHALTVFGGGASSGELVAESRSLGVPVVAGKTRIRNLADD
                     FFGGEPVLAPTNLVPLGGRTVGRQRERLQLAATLKRGEARLLTLVGEGGIGKSRLALQ
                     VAADRHANGDFPGLVAVVNADGALTLGELELRCERALTACGLGGGVGLADALGLLKGR
                     RSLLVLDDVGVGEAALGAAASLAEAALSSSPDLTVLATSVRRLAHPGEHVFRVAGLGQ
                     DGAELFFARARATTGVGAAAPDDRAGAARLSEAVGGSPLALALAAGLAGAMSPKDLAA
                     LIEREPTALGRFRGVRQRLQSPAARFAHTVAELSPGARDALAKLAYFEPGFTREAVDE
                     VTGVPATVLAELREFSLIAADADERLALGRLTRLFARGLLADAPEMRANTARAHVAYY
                     ASLPIRHYRAAFGPEQAAVQRLLHDALPNSLLAFRHALELADGGAVEAISLGCYALLG
                     RLGRFDEALTLFDEGLEALQPERPETSAARGQLLAMRAKNLVWLGRVHEAEADAERAL
                     AVLLPLGHTMGARKAYLALAECAVARQDPVAATRYFDLARGAYPLEYPGAFAGSDALV
                     QMVTGELGAAVESLQTAIEAHRRQGNAGQEAVAHFRLAGARFGRGEFGLARRSFERAM
                     KLTQGVYPYYEHASRLGLARTLVALGERVAASGLARQVAAGGPETPPDLARAAAALLE
                     HMGMGADAATAPG"
BASE COUNT          677 a         1622 c         1530 g          614 t
ORIGIN      
        1 gagcaggttc gaccagaaga cctcgaagtc gagttcgagg agttggcggt cccactcgga
       61 gcggaagccg aagaagcgcg agtcggtgaa gacggcgggc agctcggcgc tgagcgacgg
      121 ggcgagcgcg atcaccgtgt agtaggtacg agtagtgccg ttctcgaggg gcagctcggc
      181 cggctccgag taggccggag ccaagggcgt gctcatgaag tcgcccgagg ctatcgcccc
      241 caacgtggcg ccgaactcca gggagttgga gggcggaacg ctcaggtagc tgcctaccgg
      301 tacggggccg acctgcacta ccggcccgtc acccaccttg acgcccgtct cgtaggcgaa
      361 caggcgcgcg ggtcgacgct tggagttctt caggtagacg ccggtgcccg agtcggccag
      421 tagcgtctcc atgcccgcct cctcgtgcgg atcggtgatc acgacgtagt tcttgtcggt
      481 cgcggcagcc gcgagcagat cggcgggaaa ggagcgcccc gagccgccca tgccggtcgg
      541 atcagcgtcg ctcgtctgcg cggtcacgaa cgccccggca ccgctccggc ctcgcgccgc
      601 caagcgctcg cgtgcggcca tgacagcgtt cacgagggcg tcactgccat cggccagcgc
      661 gccggggtcg gctgcgagga tgctgctcaa ctcagcgtcg agggcggcta cctccggctc
      721 cacgccgagc gcctcgaaca tccgctcctg aagccacgcc tcctggaact ggccgcccag
      781 cgccatgtac aagagggcgg cggcggtact gcgacccgat agttccgcct cggcctcgcg
      841 cgtccccagg aagccaagaa gcaccaggcg gccgtcttgg ccgacgacgg ccagcaagtt
      901 gaggccatcc tcgttgacct cgactgcgaa gccgccgtcg gccgccaagt cggacgaccc
      961 gttcgcgccg agcacgcgca actcttgcgg gtcaaggggg ctgccctgcg gcaggcgcac
     1021 cttcccttcg accacgaagt cgtcctgggg gcgaaccgga ccgggcccgc cgcagccgaa
     1081 caggcccgag accgtcagta ccgtcaacag gctcgccacc gacactcgcc gcaacgccat
     1141 agccatcctc cgtggctcga cgatgaggcc ggcgtgctta atcagtgatt aacgggtgga
     1201 cagaggctct agcggcgccg ggccggacgc cgcgccggca gcgcggccgc cgtcgcgtgg
     1261 atggcctcca cgacgcgtag cggctccgcc tggagcgcgt cgggtgtcag gcgccacgac
     1321 tccttggctc ctgggtaggc aggcccgagc tcggccagac cggcgaactt cgcctcggcc
     1381 gaactccgct tgacgaagat gtcgccgtcg ttgacgaggc cgaccggctt gccgtccaga
     1441 tagaagcagt agccgccgaa catcgccctg atgcggccgc cgagcggttc gagccagccg
     1501 agtagttcat gggccaggcg ctcgctgtca gcgtccatgc ccgccgtcag tgtccatgtc
     1561 gcctctcgcc cgtccgcacg gcgtctacac cctggcgagg aacgcgagcg cgtgcctcac
     1621 gaactcgctc gtggctcccg cgtcgtactc gggggtggag ctgtccgcga agaagtgctt
     1681 gtcgcctgga tagaggaaga gctcgccgtc ggccgcctcc aagacgacag cctgcgcggt
     1741 cggcaggtcg tagccgttat cgaactccgg gtcgtgctcc tgggcgtgga tctggagagg
     1801 gacgccggcc ggccacgggc caccgaactc ctcggtgggc atgcacgcgg cgatgagtag
     1861 ggcgccgcgc gcgccggcgc gcgtctgagc gagctgctgc gcgggcatga caccgaggga
     1921 gtagcccgcg tacacgagcc cggggcccag gcctgccact gccgccaccc cacgggccgt
     1981 cacggcggcg aacccgacgg acctgacgtg ggccatgccc tcgtccagcg tcgcgaacgt
     2041 cttcccttcg tacatgtcgg gcgtatgcac ggtgtgtccg ccggcacgaa gctcatcggc
     2101 caacgcgacg atccccggtg tcagcccgag cacgtggtgg aacaggacca actctgccat
     2161 gacgactcct tggtggcgag cctagccggg tgcggtcgcc gcgtcggcac ccatgcccat
     2221 atgctcgagc agagcggccg cggcgcgggc gaggtcgggg ggtgtctcgg ggcctccggc
     2281 cgccacctgc cgcgcgaggc cgctcgcagc gaccctctcg cccagcgcga cgagcgtgcg
     2341 cgccagcccc aggcggctgg catgctcgta gtacgggtat accccctgcg tgagcttcat
     2401 cgcgcgttcg aaactacggc gcgccaagcc gaactcaccg cgcccgaaac gcgccccggc
     2461 gagcctgaag tgcgcgaccg cctcctgccc ggcgttgccc tggcggcggt gggcctcgat
     2521 ggccgtctga agcgactcca cggcagcgcc gagttcgccc gtgaccatct gcaccagcgc
     2581 gtcgctgccg gcgaaggccc ccgggtactc gagcgggtag gcccccctcg ccaggtcgaa
     2641 gtagcgggtg gctgcgacgg ggtcttgccg cgccacggcg cactcggcca gcgcaaggta
     2701 cgccttgcgg gcgcccatgg tgtgaccgag cgggaggagc acagcgagtg cgcgctccgc
     2761 atcggcttcc gcctcgtgca cccggcccag ccagaccagg ttcttcgccc gcatggcgag
     2821 gagctgtccg cgagcggcac tggtctcggg ccgttccggt tgcaacgcct ccagcccttc
     2881 gtcgaagagc gtcagcgcct cgtcgaaccg cccgagccgc cccaagaggg cgtagcagcc
     2941 gagcgatatg gcttcgaccg cgccaccgtc cgccagctcg agcgcatgcc tgaacgccag
     3001 caagctgttg ggcaacgcgt cgtgcaggag ccgctggacc gcggcctgct ctggtccgaa
     3061 ggcggcccgg tagtgccgga tgggcagact cgcgtagtaa gcgacatgcg cacgggcggt
     3121 gttcgcccgc atctcgggcg cgtcggcaag cagtccccgc gcgaacagcc gcgtgagccg
     3181 accgagcgcc aagcgttcgt ccgcatctgc ggcgatcaac gagaactccc gcagctcggc
     3241 caagacggtg gccgggaccc cggtgacctc gtcaacggcc tcgcgtgtga agcctggctc
     3301 gaagtacgcg agcttggcga gcgcgtcgcg agcgcccggg ctcagctcgg cgaccgtgtg
     3361 cgcgaaccgc gccgccgggc tctgcagtcg ctgccggacg ccgcgaaacc ttccgagcgc
     3421 ggtcggctca cgctctatca acgccgccag gtccttgggg ctcatggctc cggccagccc
     3481 ggcggccaac gccagtgcca gcggcgagcc gccgaccgcc tcggacagtc tcgcagctcc
     3541 ggcgcgatcg tcaggcgcgg ccgcgccaac gccggtggtt gcccgcgcgc gcgcgaagaa
     3601 cagctcggcg ccgtcctgac cgagaccggc caccctgaag acgtgttcac ccgggtgcgc
     3661 aaggcgccgg acgctggtgg cgagaacggt caggtccggc gacgagctca gcgccgcctc
     3721 cgccagcgag gcggcggcac ctagcgcggc ctcgcccacg cccacgtcat ccaacacgag
     3781 tagggagcgg cgccccttga ggagtcccaa cgcgtccgcg aggccgactc cgccgcccaa
     3841 cccacaagcc gtcagggcgc gctcgcagcg cagctcgagt tctccgagtg tgagcgcgcc
     3901 gtcggcgttc accacggcga cgaggcctgg gaagtcgccg ttggcgtgac ggtcggcagc
     3961 cacctgcagc gcgagcctcg acttgcctat gcccccctcc ccgaccaggg tcaacagccg
     4021 cgcttcaccc cgcttgagag tggcggcgag ttgcaggcgc tcgcgttgcc gaccgaccgt
     4081 cctgccgccc agcgggacga ggttcgtagg tgccagaacc ggctcacctc cgaagaagtc
     4141 gtctgccagg tttcggatcc ttgtcttgcc ggctacgacc ggcacgccaa gagagcgcga
     4201 ctcggccacc agctcgcccg acgacgcccc gcccccgaag acggtgagcg cgtgtagcgc
     4261 ttccaggcag gcagggtcgc taccgccctg gccggcggcg gcgtaggccg cgtcggcgag
     4321 cgcgccggcc tgagcccgcc tgcctgctgc cgctagtctg cgagcttccg ccaccgcgga
     4381 gcgctgcatg cgttccacga gcgcctctcg caccgccgtc acccaagtca ggagttcctc
     4441 tcc
//
LOCUS       DAOQYE010000048         4412 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_20222_length_4412_cov_3.493918, whole genome shotgun sequence.
ACCESSION   DAOQYE010000048 DAOQYE010000000
VERSION     DAOQYE010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 4412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4412
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20222_length_4412_cov_3.493918"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..996
                     /locus_tag="PKN52_01555"
     CDS             <1..996
                     /locus_tag="PKN52_01555"
                     /inference="COORDINATES: protein motif:HMM:NF017745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HNQ98653.1"
                     /translation="DGYLDFLFAEVHQLPMSVKEAEELALEGFERARTALEAKARAAS
                     ATEQWQAQLARLEEHHPTLADVTATYSELHHEAMEQAERAGLVTPALEYGLRFAHLPE
                     WARGVAGDLYFLFYRSPAALSPGTASPYWVFPPGEDTDAYLRSQSYATIKSTHAVHHG
                     SIGHHTQNARARQSAVLLGKLAGTDCASGIALLSGGTLIEGWACYSQELLLEAAGFYS
                     PAEELLLAHAELRNTAMCLADIRLHTGSWSLAQMRAFYEDEVGIPPGRAWTETTRNSM
                     WPSTRSMYWLGTRAIRQFRRELGGEPRRFHDDLLAFGSVPVYRVGEELVRAKERA"
     gene            993..1877
                     /locus_tag="PKN52_01560"
     CDS             993..1877
                     /locus_tag="PKN52_01560"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HNQ98654.1"
                     /translation="MTTFGGVHTIMPTPFTEDGALDLPSLESLTEFLIGRGVDGVVVL
                     GVLGEAPKLSQQEQDEVITTTVRAAAGRVPVFAGAGAGGTDLAVERARNAVALGAAGL
                     LVAPPPVQNDAVIYEYYRRVDAAVDNPLILHDYPAATGVSMTVPLVERLHAELRNVSV
                     IKLEDTPSVTKVSALRALGSDIAIVGGLGGLYFLEELERGANGIMTGFSYPEVLVAIY
                     REFTGGDRAAARRIFYEACPLLRYEFQPGIGLALRKEVYRARGAIRSAYVRHPGAQID
                     PVAQRELVNVIAHVWPNG"
     gene            1858..2907
                     /locus_tag="PKN52_01565"
     CDS             1858..2907
                     /locus_tag="PKN52_01565"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNQ98655.1"
                     /translation="MSGRTANGVRVAQVRWGVLSTANIGAKAVAPAIARSANGRLLAV
                     ASRSGDKAARYAETFGMERHYGSYQALLADQDIDAVYIPLPNSLHLEWTIKALESGKH
                     VLCEKPLALSVAECLRMEEAARLSGRVLMEAFMYRFHPRIALARELVASGRLGTVRLI
                     RAAFGFTVADPTNIRLQPELGGGSLMDVGCYCVDVARLLLAAEPDEASARAVWSGSGV
                     DVHLIGRLGFGNACLSFDCSLDTVRQEYVEVVGTEGRLRLDKALLPGTAATAIDLVTR
                     AEGETRSEVQGVDEYQLMVERFADAVLAGSAPDYTARHAAAGMAALEALYASAHSRGE
                     TVPVTPLPTNLPPPP"
     gene            complement(2870..3805)
                     /locus_tag="PKN52_01570"
     CDS             complement(2870..3805)
                     /locus_tag="PKN52_01570"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNQ98656.1"
                     /translation="MSLVVNGKRADRIIQGTAQLSSLTQAQANALLDDVAAAGATTFD
                     LAENYGGGKSEALFGGWLAGRGNRDDLFIITKGAHPYGGRNRVTSPDIGADIAGSLER
                     LGCGFVDLYLLHRDDESVPVDEVVDVLQGLKARGLVNAYGLSNWRTQRIAAANEYAKA
                     RGYDPVSASSPHFSLAVPTESPWPGCVSLSGSDGAAERAFYAATGMPVLAWSSLAMGY
                     FVVPAPGATTAAGLNEAVRVFDTPANAARRARAEELGVRLGLSAPQVALLYVLSQPFD
                     THAIVGCRSGDEYRELRVLAERRLTAAEVGWLEGA"
     gene            complement(3840..>4412)
                     /locus_tag="PKN52_01575"
     CDS             complement(3840..>4412)
                     /locus_tag="PKN52_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HNQ98657.1"
                     /translation="RAAHAGRVTLQMSMSGPEWRVFAANAEEVARIVQGETGLRTVFH
                     PHCAGFIETPDEVERLLDLTDAELMGLVFDTGHHVYGSAKADEDGQSAVLGLERFWDR
                     VWYVHFKDCDGTVAERARREGWDLRQAVRAGVFAELGKGTVDFAAVLDVLRRRGYSDW
                     ITVEQDVLPGMGTPKESAGRNRAFLRGLGL"
BASE COUNT          691 a         1535 c         1515 g          671 t
ORIGIN      
        1 gacggttacc tcgacttcct gttcgccgag gtgcaccaac tgcctatgtc ggtcaaggaa
       61 gccgaggaac tggccttgga aggcttcgag cgcgccagaa ccgcgctgga agcgaaagcg
      121 cgggccgcga gcgccaccga gcagtggcaa gcgcaactgg cgaggctcga ggagcaccac
      181 ccgaccctgg ccgacgtgac agcgacctac tccgaactgc accacgaggc catggagcag
      241 gccgagcgcg ccggtctcgt gacgccagcg ctcgagtacg ggttgcggtt cgcgcacctg
      301 cccgagtggg cgcggggagt ggccggcgat ctctacttcc tcttctaccg gtcgccggcc
      361 gcgttgagcc ccggaacggc cagcccgtac tgggtgttcc ctcccgggga ggataccgac
      421 gcgtacctga ggagccagag ctacgcgacc atcaagagca ctcacgccgt ccaccacggc
      481 tcgatcggcc accacacgca gaacgcgcgc gccaggcagt ccgcggtctt gctcgggaag
      541 ttggcgggca ccgattgcgc ttccggcatc gccttgctgt cgggaggcac cttgatagag
      601 ggttgggcct gttactcgca ggaactgctg ctcgaggcag ccggcttcta ctcgccggcg
      661 gaggagctgc tgctggcgca cgccgagctc aggaacaccg ccatgtgcct cgccgacatc
      721 aggcttcaca cggggagctg gagtctcgcg cagatgcgcg ccttctacga ggacgaggtc
      781 gggatccctc ctgggcgcgc ctggacggag accaccagga actccatgtg gccgagcact
      841 aggtccatgt actggctcgg cacgcgggcc atcaggcagt tccggcggga actcgggggc
      901 gaaccgcggc ggttccacga cgacctgctg gcgttcggtt ccgttcccgt ctaccgcgtc
      961 ggcgaagagc tggtacgcgc gaaggagcga gcatgacgac gttcggtggc gtccacacca
     1021 tcatgcctac gccgttcacg gaggacggcg cactcgacct gcccagcctg gagagcctga
     1081 ccgagttcct gataggtcgc ggggtcgacg gcgtagtggt gttgggcgtc ctgggcgagg
     1141 cgcccaagct ctcacagcaa gagcaggacg aggtgataac gacgacggtg cgggcggcgg
     1201 cgggcagggt acccgtgttc gccggggccg gagcgggcgg caccgacctc gcggtggagc
     1261 gagccaggaa cgccgtagcg ctcggcgccg ccggactgct ggtcgcaccg ccgcccgtgc
     1321 agaacgacgc cgtcatctac gagtactacc ggcgggtgga cgcggccgtc gacaacccgc
     1381 tcatccttca cgactacccg gccgccacgg gcgtgagcat gaccgtgccg ttggtggaac
     1441 ggctgcatgc ggaactccgg aacgtgagcg tgatcaagct cgaggacacg ccgagcgtca
     1501 cgaaggtctc ggcgctacgc gccctcgggt cagacatagc gatagtgggc gggctcggag
     1561 ggctctactt cctcgaggag ttggaacggg gcgccaacgg catcatgacc ggcttctcgt
     1621 accccgaggt gttggtcgcc atctaccggg agttcaccgg cggcgacagg gcggcggccc
     1681 gccgcatctt ctacgaggcc tgtccgctcc ttcggtacga gttccaaccc ggcatcggac
     1741 tcgccctgcg caaggaggtc taccgggcac ggggtgcgat aaggagcgcc tacgtgaggc
     1801 accccggcgc ccagatcgac cccgtcgccc agcgcgagct cgtcaacgtg atcgcgcatg
     1861 tctggccgaa cggctaacgg cgtgcgcgtg gctcaggtcc gttggggggt gctaagcacc
     1921 gccaacatcg gggcgaaggc cgtggcgccg gccatcgccc ggtccgccaa cggccgcttg
     1981 ctcgcggtcg ccagcaggag cggcgacaag gcggcgcggt acgcggagac cttcggcatg
     2041 gagcgtcact acgggtcgta tcaggcgttg ctggccgacc aggacatcga cgccgtctac
     2101 atccccctgc cgaacagcct ccacctcgag tggaccatca aagccctcga gagcggtaaa
     2161 cacgtgctat gcgagaagcc gctcgcgctg tcggtcgccg agtgcctacg catggaggaa
     2221 gcggcccggc tctcgggacg ggtgctcatg gaggcgttca tgtaccgctt ccatcctcgc
     2281 atcgccctgg cccgcgagct cgtcgccagc gggcggctgg gaaccgtacg cctgatccgc
     2341 gccgccttcg gcttcaccgt tgccgatccg accaacatcc gcctgcagcc ggaactaggc
     2401 gggggttcac tgatggacgt cggctgctac tgcgtcgacg tcgcccgcct cctcctcgcc
     2461 gccgagccgg acgaggcctc ggcacgcgcc gtctggtcgg gctccggcgt cgacgtccac
     2521 ctcatcggcc ggcttgggtt cgggaacgcg tgcctgagct tcgactgctc gctggacacg
     2581 gtgcgacagg agtacgtcga ggtggtaggg accgagggcc ggttgcgcct tgacaaggcc
     2641 ttactacccg gcacggcggc gaccgccatc gacctggtca ccagggcgga aggcgagacg
     2701 aggtccgagg tccagggagt ggacgagtac cagctgatgg tcgagcgctt cgccgacgcg
     2761 gtgctcgccg gcagcgcgcc tgactacacg gctcgtcacg cagccgccgg catggccgca
     2821 ctggaggcgc tatacgcctc ggcccactcc aggggcgaaa ccgtgccggt cacgcccctt
     2881 ccaaccaacc tacctccgcc gccgtgagcc gtcgctccgc caggacccgc agctcgcggt
     2941 actcgtcccc ggaccggcac ccgacgatgg cgtgcgtgtc gaacggctgg ctgagcacgt
     3001 agaggagcgc gacctgcgga gcgctcagcc ccagtcggac gccgagttcc tctgcccgcg
     3061 cgcggcgcgc ggcgttggcc ggcgtgtcga acaccctcac ggcctcgttc aggccggcgg
     3121 ccgtcgtggc gccgggcgcg gggacgacga agtaacccat ggccaggctc gaccaggcca
     3181 agaccggcat gccggtcgcc gcgtagaagg cgcgttcggc ggcgccgtcc gaacctgaca
     3241 ggctgacgca gcccggccag ggcgactccg tcggcaccgc caagctgaag tgcgggctgc
     3301 tggcgctcac cgggtcgtag cccctggcct tggcgtactc gttggccgcg gcgatacgct
     3361 gggtgcgcca gttcgagaga ccgtaggcgt tcacgagccc tcgcgccttg aggccttgca
     3421 acacgtccac gacctcgtcg accggcacgc tctcgtcgtc gcggtgcagg aggtagaggt
     3481 cgacgaagcc gcaaccgagg cgctcgagtg acccggcgat gtcggccccg atgtcggggc
     3541 tggtcacgcg gttgcgcccg ccgtaagggt gggcgccctt cgtgatgatg aagaggtcgt
     3601 cgcggttgcc gcgccccgcc agccaccccc cgaagagcgc ctcgctcttc ccgcccccgt
     3661 agttctcggc caggtcgaag gtcgtggcgc cggctgccgc cacgtcgtcg agcagagcgt
     3721 tcgcctgcgc ctgcgtaagg gacgagagct gggcggtgcc ctggatgatg cggtctgctc
     3781 tcttaccgtt cacgactagg ctcatgcgca gcacctcacg gtctcgagac tatggggact
     3841 cacaggccca gccctcgtag gaacgcgcgg ttgcgcccgg cgctctcctt gggtgtgccc
     3901 atgcctggga gaacgtcctg ctcgacggtt atccagtcgg agtaccccct gcgtcgcaac
     3961 acgtcgagga ccgccgcgaa gtcgacggtg cctttcccga gctccgcgaa cacgccggcg
     4021 cgcacggcct gccgcaggtc ccacccctcg cgccttgccc gttcagccac ggtcccgtcg
     4081 cagtccttga agtgcacgta ccagacccgg tcccagaagc gttccaagcc cagaacggcg
     4141 ctctggccgt cctcatcggc cttggcgctg ccgtacacgt ggtggcccgt gtcgaacacg
     4201 aggcccatga gctcggcgtc cgtcaggtcg aggaggcgct cgacctcgtc gggcgtctcg
     4261 atgaagccgg cgcagtgcgg atggaacacg gtccgcaacc ccgtctcgcc ttgaacgatc
     4321 cgggcgacct cctcggcgtt cgccgcgaac accctccact cgggcccgga catgctcatc
     4381 tggagcgtga cgcgtcctgc gtgggcggcg cg
//
LOCUS       DAOQYE010000049         4393 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_20340_length_4393_cov_3.278700, whole genome shotgun sequence.
ACCESSION   DAOQYE010000049 DAOQYE010000000
VERSION     DAOQYE010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 4393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4393
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20340_length_4393_cov_3.278700"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1345)
                     /locus_tag="PKN52_01580"
     CDS             complement(<1..1345)
                     /locus_tag="PKN52_01580"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNQ98658.1"
                     /translation="MRRLLILVIAVLAWSANAQTLTIAISEGIPGFDPTQTTRTVAND
                     VYPNIFDGLLALDREGQIVPSLALSWEVVNEKDWHLTLRQGVKWHDGETFDAEDVKFT
                     LERLATNQDLIRHVLMTELESVEVVNPYEVVLHMANPDPLLPVNLTMPATAMLPAHYF
                     EAVGVDKFTQAPIGTGPYKFTEYRPDDRLVLTVNEDYWAGKAAFSELVFRVITESTTS
                     VSELITGGVAVSDIGPNDLDRVESSGVAHVTTQATNRVVHWTLNVSEGQATADKRVRE
                     ALDLAIDNQVFIDVLENGYGTPTRVRTGPGDNFMPTKYYGTYNYDPDRARELLAEAGY
                     GPGELTITIMGASSVSDRAELTAAMLEAVGIVPDIQLFESSVWSSKYWTPGEFTNMAG
                     VGSSNSAMDYGSTLSDLQCPEGAHSQRSHWCNEEFSDLVRAANRELDTAKRAELLD"
     gene            complement(1403..2101)
                     /locus_tag="PKN52_01585"
     CDS             complement(1403..2101)
                     /locus_tag="PKN52_01585"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNQ98659.1"
                     /translation="MITPRTRAFVFNNPNNPTGTVFTREELAEVASVLAEHGILAIVD
                     EIYEEFVYDGRRHVSLAALPGAREHVLLINGYSKAYAMTGWRVGYAAGPSWLIDRMLV
                     IHQHLISSPCSFAQKGASVAYSEAADHVRAMVETYDRRRMLLNEALARVPKIRYSLPE
                     GACFFFLRIDSRLDSATLARTIASREGLVLTPGGAFGPTGEHHLRFSFAALPEPRVGE
                     AVNRLAAALEAIVE"
     gene            complement(2098..2604)
                     /locus_tag="PKN52_01590"
     CDS             complement(2098..2604)
                     /locus_tag="PKN52_01590"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNQ98660.1"
                     /translation="MLVGEGPLNPLPLPTRLRSVKPKATNTAAEGIPSLEFGEREILS
                     LATARPDLDTPAVVKAAVIAAIQEPHKYMTYAPSRGLPELRASVAHKLSTRNGVDYDP
                     DSELLITAGTHEALYVSPQAVVEPGDEVLVLDPSWVAYSGMIGLAGGVPVMVGLRENR
                     LDIDDVGA"
     gene            3002..4006
                     /locus_tag="PKN52_01595"
     CDS             3002..4006
                     /locus_tag="PKN52_01595"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HNQ98661.1"
                     /translation="MTTPISVLDLVPVPPGATAGEAIDHSIELARLAEELGYRRYWVA
                     EHHNTPSFASMATSVLIGVLARETSRIRVGSGGIMLPNHSPLKVAEDFLTLEAMHPGR
                     IDLGLGRAPGTDQVTALALRRSTELLAADDYPQQVEMLRAYAGESDFLAGHPLGKVSA
                     ALPGARLPPLWILGSSTYGAQFAAQTGRGYSFAFHFSPDAAVPALRAYREGFRPSDEL
                     AEPYAILGVSVVCADTVERAEVLAHAHDLLWLRIRRNERLPLPTPDEAAAYRFTAEEK
                     AQAQASRRMLVSGTPDQVRKRLLALKDELAVDEFIVTSHIATHEERLASYRLLAEAMA
                     "
BASE COUNT          676 a         1404 c         1491 g          822 t
ORIGIN      
        1 cgtcgaggag ttctgcgcgc ttcgccgtgt ccagctcgcg gttggcggcg cgcacgaggt
       61 cgctgaactc ctcgttgcac cagtggctgc gctgggagtg ggcaccttca gggcattgaa
      121 ggtcgctgag ggtggagccg tagtccatgg cggagttgct cgacccgacg ccggccatgt
      181 tcgtgaactc gcccggcgtc cagtacttgc tcgaccagac gctgctctcg aacagttgga
      241 tgtccgggac gatcccgacc gcctcgagca tggccgcggt gagctcggcg cggtcgctga
      301 cgctgctggc gcccatgatc gtgatcgtca gctcgcccgg gccgtagccg gcttcggcga
      361 ggagttccct ggcgcgatcg gggtcgtagt tgtaggtgcc gtagtacttg gtgggcatga
      421 agttgtcgcc cgggccggtg cgtacgcgcg tgggcgtgcc gtagccgttc tcgaggacat
      481 cgatgaacac ttggttgtcg atggcgaggt cgagcgcctc gcggacgcgc ttgtcggccg
      541 tggcctgccc ctcggacacg ttcagcgtcc aatggacgac gcggttcgtc gcctgggtcg
      601 tgacgtgcgc caccccgctc gactcgaccc ggtcgaggtc gttggggccg atgtcggaca
      661 cggcgacgcc accggtgatg agttcgctca cgctggtcgt cgactcggtg atcaccctga
      721 acacgagttc gctgaaggcc gccttgccgg cccagtagtc ctcgttgacc gtcaggacga
      781 gccggtcatc cgggcggtac tcggtgaact tgtaggggcc ggtgccgatg ggcgcctgcg
      841 tgaacttgtc gaccccgacg gcctcgaagt agtgcgcggg cagcatggcg gtcgccggca
      901 tggtcaggtt gacgggaagg agcgggtccg ggttcgccat gtggaggacg acctcgtacg
      961 ggttgactac ctccaccgac tccagctccg tcatcagaac gtgcctgatc aggtcctggt
     1021 tggtggccag gcgctcgagc gtgaacttga cgtcttcggc gtcgaacgtc tcgccgtcat
     1081 gccatttgac gccttggcga agcgtgaggt gccagtcctt ctcgttgact acctcccaag
     1141 agagcgccag cgacggcact atctggcctt cgcggtccag agccagcagc ccgtcgaaga
     1201 tgttcgggta gacgtcgttg gccacggtcc tggtcgtctg cgtgggatcg aagcccggta
     1261 ttccctcgct gatggcaata gtcaacgtct gggcgttcgc gctccaagcg agcacggcga
     1321 tcactagtat caacagtctt ctcatcaatt gcaccgcctt cttgacatgg gttcaggttt
     1381 gccgtccgcg gggaagttag ggctactcca cgatggcctc caaagctgcc gcgagacggt
     1441 tcacggcttc gcccacccga ggctcgggca acgccgcgaa ggagaacctc aagtgatgct
     1501 cgccggtcgg gccgaacgcg ccgcctggag tgagcacgag gccttccctg ctcgcgatcg
     1561 ttcgggcgag cgtggcggag tcgaggcggg aatcgatgcg caggaagaag aaacacgcac
     1621 cttcggggag gctgtacctg atcttgggga cccttgccaa cgcttcgttc agcagcatcc
     1681 gccgccggtc gtacgtctcc accatcgcgc gtacgtggtc cgccgcttcg gagtaggcca
     1741 cgctcgcgcc cttctgcgcg aacgaacacg gcgacgagat caggtgctgg tggatgacga
     1801 gcatccggtc tatcagccag ctcggtcccg cagcgtagcc gacccgccag ccggtcatgg
     1861 cgtacgcctt cgaatagccg ttgatgagca gcacgtgctc gcgcgcgccc gggagcgccg
     1921 ccagactgac gtgccgcctc ccgtcgtaga cgaactcctc gtagatctcg tcgacgatgg
     1981 cgaggatgcc gtgctccgcg agcacgctcg ccacctccgc gagttcctcg cgggtgaaca
     2041 ccgtgcccgt cgggttgttg gggttgttga agacgaaggc ccgcgttctt ggggtgatca
     2101 cgcgccgaca tcgtcgatgt ccagtcggtt ctcacggagg ccgaccatga ccggcacgcc
     2161 gcccgccagt ccgatcatcc cgctgtacgc gacccagctg gggtccagaa cgagcacctc
     2221 gtcgcccggt tcgacaacgg cctgcggact gacgtacagc gcctcatggg tgccggccgt
     2281 tatcagcagt tcgctgtcgg ggtcgtagtc gacgccgttg cgtgtgctga gcttatgcgc
     2341 cacggacgcg cgcagctctg gcaggccgcg cgatggggcg taggtcatgt acttgtgcgg
     2401 ttcttggatg gctgcgatga cggcggcctt gactacggca ggcgtgtcca gatccggccg
     2461 cgccgtagcg aggctcagga tctcgcgttc gccgaactcg aggcttggga taccctcggc
     2521 ggccgtgttg gtcgcctttg gctttacgct cctcaagcgt gtgggcaggg gcagagggtt
     2581 cagggggccc tccccgacca gcatctggtt tgcatagggt cagatggtat accaagaagc
     2641 ggatattatc aagcggcgtc ttagattatg ccgctaaaca aggggttgtc tcgtgaatca
     2701 tcaccgcatt ggtattcact ggcccggccc ccgaacatgt gtctacccga gacagtagca
     2761 gcgatggcgg cacgtacatc cacccaatgc tctactacca acttgtagct aagcaagcgg
     2821 gagacgacgc atgttcgaac gcgttcaccc cccgtgccgg agactaatga caaagcggtt
     2881 ggcgtcggta cgggatcggt actcgatcct gggagcggcg cgcggtcgcg cccgactgct
     2941 ggcgcaaccg gcgcgtcctt ggaccaccag tccttgcggc ggtgctgggt atcgttctcc
     3001 agtgacgaca cccatctccg tcctcgacct agtcccggtc ccgccgggag cgaccgccgg
     3061 cgaggccatc gaccacagca tcgagctggc ccggctggcc gaggagctcg gctaccgccg
     3121 gtactgggtc gccgagcacc acaacacgcc ttcgttcgcc tcgatggcca cgtctgtgct
     3181 gatcggcgta ctggcgcgcg agacgagccg catccgggtc ggttcgggcg gcatcatgct
     3241 gcccaaccac tcccctctca aggtcgcgga ggacttcctc accctcgagg ccatgcaccc
     3301 ggggcgcatc gacctgggcc taggccgggc tcccggcacg gaccaggtga cggcgctcgc
     3361 tctgcgccgc agcaccgagc tgctcgcggc cgacgattac cctcagcagg tcgagatgct
     3421 gagggcgtac gcaggcgagt cggacttcct ggccgggcat ccgctcggca aggtcagcgc
     3481 ggccctgccg ggcgcgagac tgccaccgtt atggatccta ggctcgagca cgtacggggc
     3541 gcagttcgcg gcgcaaaccg gtcggggcta ctcgttcgcg ttccacttca gccccgacgc
     3601 ggccgtgccg gcgctgcgcg cctaccgcga gggtttcagg ccatcggacg agctggctga
     3661 gccgtacgcc atcctcggcg tgtcggtcgt gtgcgccgat accgtggagc gcgccgaggt
     3721 gctcgcccac gcccacgacc tactgtggct acgcatccgc cggaacgaac gcttgccact
     3781 gccgaccccg gacgaggcag cggcctaccg gttcacggcc gaggagaagg cgcaggccca
     3841 ggcgtctcgt cggatgctcg tgtcgggcac gccggaccag gtcaggaagc gtctgctcgc
     3901 cttgaaggac gagctcgcgg tggacgagtt catcgttacc agccacatcg cgacgcacga
     3961 ggaacggctc gcttcctacc ggctgctggc tgaggctatg gcgtagtcag cggggcctag
     4021 gaagcgattc cgcgggtggc cgccggttct tagcgtcctg gagtcggtgg acgccgaccc
     4081 tcggcctggg aggttcatcg tctccggttc ggagaacctg gctttgacca acagcgtgag
     4141 ccagtcgctc gctggacgca ccgcgatgct cgtcctcctc cccttttctc cggaagagcg
     4201 ggtactaggg cgtgttcgca ctatgttgtg tggtgtttcc gatgggtagg caggcgtagc
     4261 gggggcgggg tagcggggcg ggaccggttg gtggcgtctt cgtgagcgtc gcgttcgtcg
     4321 ctgactgact acgaggcatg ggtttctccc ctgatgaggt gccggagtgc ggtctaggcg
     4381 ttcgggtcga gcg
//
LOCUS       DAOQYE010000050         4348 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_20620_length_4348_cov_3.472630, whole genome shotgun sequence.
ACCESSION   DAOQYE010000050 DAOQYE010000000
VERSION     DAOQYE010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 4348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4348
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20620_length_4348_cov_3.472630"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(128..1012)
                     /locus_tag="PKN52_01600"
     CDS             complement(128..1012)
                     /locus_tag="PKN52_01600"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ98662.1"
                     /translation="MSDAAGSLIQAGKDDARKAQRARFWRRVRRQPVGIVALAVLVLL
                     AAAVWLGPLAYRTAPNRTDARNILKAPTAAHPLGTDELGRDVLARVLAGGRVSLSVGL
                     LAMVVGLAIGTLAGGLAGYFRGRVEVVVMRLVDVFMAIPNFFLILVVVTAFGNHPVTV
                     IVTVGIGFWAQMARVVHAEFVKVRGFEFVEAARALGATHPRTMLRHVMPQVVPQAIVL
                     ASLGVGWAILTEAGLSYLGLGIQPPLASWGNMLQNAQSYLWLKPELAIYPGLFIAATV
                     LAFNVLGNALRDVFDPRG"
     gene            complement(1005..1952)
                     /locus_tag="PKN52_01605"
     CDS             complement(1005..1952)
                     /locus_tag="PKN52_01605"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ98663.1"
                     /translation="MTAFIVRRVLMGVVVLWLVSVVTFFLIHLAPGGPSAAISFDSTA
                     EQREALLKQFGLYDPPLVRYGKWIVGTVRGDLGVSYNQGLPVGQLLMQRLPNTLTLGL
                     AALVAATLLGLLLGTLAAVYRNGWVDHVVSTISTLGMSIPGFWLGIVAIILFAVQLRW
                     LPASGIVSSSGAGSLVDRLRHLVLPAGVLAFTLMPNVVRVTRSALLEVNRADYVRTAK
                     GKGLAPGTVLFKHAFKNALVPVLAVLGLVTTALFSGSVVIESVFGWPGLGRLAIEAAN
                     GRDYPVILGATLLAGLIVVVVNLLVDLLYAAVDPRIRHE"
     gene            complement(1975..3573)
                     /locus_tag="PKN52_01610"
     CDS             complement(1975..3573)
                     /locus_tag="PKN52_01610"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNQ98664.1"
                     /translation="MPPIRVHLKLLKVLAALLASLAVALASAQDHDPNGSLTFATNAD
                     PTLNPWNASAVIESNLINTILFDQLVRYDPTDLHPAPGLAESWETAADGLSWTFHLRP
                     EAKWSDGVPFTADDVVFTFNDAILNKDLGARNASGFAPVDRVVKVDDHTVVFELNRPF
                     SSLPYFLAYYAGIIPAHVLGDAENPLTVASFNKGNPVVTGPYRVGEFVPGAYVKLVPN
                     ENYWGPAPKLASITFNIIPDGNAQIAQLLAGGLDMVSITNPALLAGVANNPNLEVIRQ
                     SQNIWYFVALNLNDERFQDVRVRQALLSAIDRPAIIDAVLEGYGVVATGPIAPLQQAL
                     YDDEVAKWPYDPEKAKELLAEAGWTPGPDGVLQKDGQRFVIDMPTGQYGYLVPSTLLV
                     QQYWQDIGVEAKVETIEWNAFIQKAVVNRDYDAMLAWWSTPPTPDQSSYFASSGAVTG
                     NNIPDYRDPVLDEIWERGRAATSQEEQVAVYREAQERMAEQLPYLFLWYPDMIAVKNT
                     RIGGMHDINSAANFQYSYDWYVTR"
     gene            complement(3594..>4348)
                     /locus_tag="PKN52_01615"
     CDS             complement(3594..>4348)
                     /locus_tag="PKN52_01615"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HNQ98665.1"
                     /translation="QSALRTLQVLLAFGKPPHRFGLSDLLLVTPFEKNQLYRSLRTLE
                     AAGFIVADSGGRYTVTPVVNVLSAASAHGRPLTLVEVASPHLDALADETGEAVNLNAL
                     AGDQAVCLDRRESPHAVRLASVLGQAYPLHAGAGPKAMLAFLSEELRAQVLKKVDSYP
                     NYTPATQRDPRLLQKELEQTRERGYSISDGDFDEAARGVGAPIFDASGQVVGAVSVGG
                     PSFRVGEAELARYAPLIIRVAKAISNGLGHPG"
BASE COUNT          705 a         1330 c         1642 g          671 t
ORIGIN      
        1 ctcgacggca cgcggttcag cacggggacg agctcgaacg ccttgtagtc gacgcccggc
       61 tcgaggtact ggaacgactt gtagccgtcg taacgcttct tctgcatctg tgatctcctt
      121 ggggaggcta gccgcgcgga tcgaacacgt cgcgcagcgc gttgcctagc acgttgaagg
      181 ccagcaccgt cgcggcgatg aacaggcccg ggtagatggc gagctccggt ttgagccaga
      241 ggtacgactg ggcgttctgg agcatgttgc cccaagaggc gaggggcggc tggatgccga
      301 ggccgaggta cgagagtccg gcctccgtga ggatggccca gccgacgcct agggacgcga
      361 gcacgatggc ctggggcacg acctgaggca tcacgtgcct gagcatggtg cgcgggtggg
      421 tggcgccgag cgcgcgggcg gcctcgacga actcgaagcc cctcaccttc acgaactcgg
      481 cgtgcacgac cctggccatc tgggcccaga agccgatgcc gaccgtgacg atgacggtga
      541 cggggtggtt gccgaacgcc gtgaccacga ccaggatcaa gaagaagttg gggatggcca
      601 tgaacacgtc tacgaggcgc atgaccacga cctcgacccg cccgcggaag tagccggcga
      661 gtccgccggc cagcgtgccg atggcgaggc cgacgaccat ggcgagcaga ccgacgctca
      721 gtgagacgcg cccgccggcg aggacgcgcg cgagcacgtc gcggccgagc tcgtcggtac
      781 cgagcgggtg cgcggccgtg ggggccttga ggatgttgcg ggcgtccgtg cggttggggg
      841 ccgtccggta ggcgagcggc ccgagccaga cggcggcggc gaggaggacg aggacggcga
      901 gcgcgacgat cccgactggt tggcgccgca cgcgccgcca gaagcgggcg cgctgcgctt
      961 tgcgggcgtc gtccttgccc gcttggatga gcgagccggc cgcgtcactc atggcggatc
     1021 ctagggtcga cggcggcgta caggaggtcg acgagcaggt tgacgaccac gacgatgagg
     1081 cccgccagga gcgtggcccc caggatgacc gggtagtcgc gcccgttcgc cgcctcgatg
     1141 gcgaggcgcc cgagtcccgg ccatccgaac acgctctcga tgacgaccga gccggagaag
     1201 agggccgtgg tcacgagccc gaggacggcg agcacgggta cgagcgcgtt cttgaaggcg
     1261 tgcttgaaga ggacggtgcc cggagccagg cccttgccct tggcggtccg cacgtagtcg
     1321 gcccggttga cttccaggag ggccgagcgt gtgacgcgca cgacgttggg catgagcgtg
     1381 aaggccagca cgccggccgg cagcacgagg tggcgcaagc ggtcgaccag gctcccggcc
     1441 ccactcgagc tgacgatgcc ggaggccggc agccaccgca gttgcacggc gaagaggatg
     1501 atggcgacga tccccagcca gaagccgggg atcgacatgc cgagcgtaga gatggtgctg
     1561 acgacgtggt caacccaacc gttgcggtag acagccgcca gggtccccag caacaggccg
     1621 aggagcgtgg cggcgacgag ggcggctagg cccaacgtga gcgtgttggg caggcgttgc
     1681 atgaggagct ggccgacggg gaggccctgg ttgtagctca ccccgaggtc gccccgcacc
     1741 gttccgacga tccacttgcc gtagcgcacg aggggcgggt cgtagaggcc gaactgcttg
     1801 aggagcgcct cgcgctgttc cgccgtgctg tcgaagctga tggccgccga cggcccgccc
     1861 ggggctaggt ggatgaggaa gaacgtcacg accgagacga gccacaagac gaccacgccc
     1921 atgagcaccc tgcggacgat gaacgcggtc atctatctat ggctgcctgc ggccttagcg
     1981 cgtaacgtac cagtcgtagg agtactggaa gttcgcggcg ctgttgatgt cgtgcatccc
     2041 accgatgcgt gtgttcttca cggcgatcat gtcggggtac cagaggaaga ggtagggcag
     2101 ctgctccgcc atgcgctcct gcgcctcgcg gtagacggcg acctgctcct cctggctggt
     2161 ggcggcccgg ccgcgctccc agatctcgtc gaggacggga tcgcggtaat ccgggatgtt
     2221 gttgcccgtg acggccccag aggaggcgaa gtagctggac tggtcggggg tcggcggggt
     2281 gctccaccaa gcgagcatgg cgtcgtagtc gcggttcacg acggccttct ggatgaaggc
     2341 gttccactcg atcgtctcca ccttggcctc gacgccgatg tcttgccagt actgctgcac
     2401 gaggagcgtg gaggggacga ggtagccgta ctgcccggtt ggcatgtcga tgacgaagcg
     2461 ctggccgtcc ttctggagca cgccgtccgg gccgggcgtc cagccggcct ccgcgaggag
     2521 ctccttggcc ttctccgggt cgtagggcca cttggcgacc tcgtcgtcgt agagggcctg
     2581 ctgcagcgga gcgatcgggc cggtggcgac cacgccgtag ccttcgagga ccgcgtcgat
     2641 gatggccggg cggtcgatgg cgctgaggag cgcctggcgc acgcgcacgt cctggaagcg
     2701 ctcgtcgttg aggttgaggg cgacgaagta ccagatgttc tggctctgcc tgatgacctc
     2761 gaggttgggg ttgttcgcca cgccagcgag gagggcgggg ttcgtgatcg agaccatgtc
     2821 gagcccgccg gccaggagct gcgcgatctg ggcgttgccg tcggggatga tgttgaaggt
     2881 gatggacgcg agcttcggcg ccggccccca gtagttctcg ttcggcacga gcttcacgta
     2941 ggcgcccggc acgaactcgc cgacgcggta cgggcccgtg accaccgggt tgcccttgtt
     3001 gaacgaggcc accgtgagcg ggttctcggc gtcgccgagg acgtgcgctg ggatgatccc
     3061 ggcgtagtag gcgaggaagt acggcaagga ggagaacggt cggttcagtt cgaagacgac
     3121 ggtgtgatcg tcgaccttga cgaccctatc gacgggcgcg aagcccgagg cgttccgcgc
     3181 gccgaggtcc ttgttgagga tggcgtcgtt gaacgtgaac acgacgtcgt cggccgtgaa
     3241 cggcacgccg tcggaccact tggcttcggg gcgcaggtgg aaggtccacg agaggccgtc
     3301 cgccgccgtc tcccagctct ccgccaggcc cggcgcgggg tggagatcgg tcgggtcgta
     3361 gcgcacgagc tggtcgaaga ggatggtgtt gatgaggttg gactcgatga ctgcggaggc
     3421 gttccaaggg ttgagcgtgg gatcggcgtt cgtcgcgaag gtcagagacc cgttggggtc
     3481 gtggtcctgc gcgctcgcca gtgcgaccgc caacgacgcc agcagcgctg ccagcacctt
     3541 gagaagcttg agatggacac ggatcggggg cattcggtcc tccttggatt tcctcacccc
     3601 gggtgcccga ggccgttcga gatggccttg gctacccgga ttatgagtgg ggcgtaacgc
     3661 gccagctccg cttcgccgac gcggaaagac ggtcccccga cgctgacggc gccgacgacc
     3721 tggccggagg cgtcgaagat gggcgcgcca accccccggg cggcctcgtc gaagtctccg
     3781 tcgctgatgg agtagccgcg ctcgcgcgtc tgctcgagct ccttctggag gagccgaggg
     3841 tcgcgctgcg tggccggggt gtagttgggg taggagtcga ccttcttgag aacctgagcg
     3901 cgcagctcct cgctcaagaa cgcgagcatg gccttggggc cggcgcccgc gtgtaggggg
     3961 tatgcctgcc cgaggacgga ggcgaggcgc acggcgtgcg gactctcgcg ccggtcgagg
     4021 cagacggcct ggtcgccggc gagggcgttc aggttgacgg cctcgcctgt ctcgtccgcg
     4081 agcgcgtcaa ggtgaggcga ggcgacctcg acgagggtca ggggccggcc gtgtgccgaa
     4141 gcggcgctca ggacgttcac caccggcgtg accgtgtagc ggccgccact gtccgccacg
     4201 atgaagccgg cggcctcgag ggtgcgcagg gagcggtaga gctggttctt ctcgaacgga
     4261 gtgacgagca ggagatcgga tagcccgaac cggtgcggcg gcttcccgaa cgccagcagg
     4321 acctgtaggg tccgcaaggc cgactgga
//
LOCUS       DAOQYE010000051         4347 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_20633_length_4347_cov_2.539143, whole genome shotgun sequence.
ACCESSION   DAOQYE010000051 DAOQYE010000000
VERSION     DAOQYE010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 4347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4347
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20633_length_4347_cov_2.539143"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /gene="fabZ"
                     /locus_tag="PKN52_01620"
     CDS             complement(<1..396)
                     /gene="fabZ"
                     /locus_tag="PKN52_01620"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: protein motif:HMM:NF000582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HNQ98666.1"
                     /translation="MAVDIRGILPHRYPFLLVDAFVKLEGDSFECLKNVSNNEPFFVG
                     HFPQEPVMPGVLILEALAQAAAVGVAVREGLVGGGPAGDGPTKVGYLTGVEAAKFRRK
                     VVPGDQLHLTGTIVLFRRGLCKVEARALVG"
     gene            complement(402..1397)
                     /locus_tag="PKN52_01625"
     CDS             complement(402..1397)
                     /locus_tag="PKN52_01625"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNQ98667.1"
                     /translation="MSTTDQQPDALLRVAVVGAGVMGRYHATNYATLPRVRIVAVVEV
                     DEARRREAQLAYGCAGYATLAELLANEVVDAASVAVPTSLHYPVTKALLEAGVHVLVE
                     KPVATDVAQATALARLSEAQGLVLQVGHITRFYSAVQMLAREVRQPYLIEARRLSPSQ
                     RVKDVGVVLDLMIHDIDIVFGLMGSGDRVVDVVASGLPMNGSPLEDVAIAQIRFEGGC
                     LVRFLASRVSPDPERTMLIAERDKTVRVDFAKEPYSEVQFFRTPGSAAHSGHVKLDRH
                     LVHDENPLRKELEHFLARVRGTTDPIGTLADDMRSLELATRILTAMREGAGVPVH"
     gene            complement(1394..2164)
                     /locus_tag="PKN52_01630"
     CDS             complement(1394..2164)
                     /locus_tag="PKN52_01630"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:NF015299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HNQ98668.1"
                     /translation="MVTVTGRAIHCGLTSSVTLHRVDGPIRFLRGGTEVPADLGHLAG
                     AERATSLAADGANVHLVEHLLAALRVAGFFEGVLIEATCDELPILDGSAEPWAAAVHK
                     LGPAPTAPAAIALGREVEVALRGGRARATPGEESLAYRVAFDHPAIGEQAWEGGPADY
                     PGLLSARTFGFERDWEALKARGLALGASEEHVIVFAMDGPTRPLRHPDEPVRHKALDA
                     LGDLALLGRPLRARLTIERGSHALHHALALAVKLAATA"
     gene            complement(2171..3322)
                     /gene="lpxD"
                     /locus_tag="PKN52_01635"
     CDS             complement(2171..3322)
                     /gene="lpxD"
                     /locus_tag="PKN52_01635"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HNQ98669.1"
                     /translation="MTETRARTYGELVAALVSGAGVTGTGAAVGATGPTGGASIRRLV
                     DASVTRQAGVDLADAACVMSKEAAAAGPPASRPALLVVPSGTSWAADYGTGLAQAPDA
                     RLALAVLSACFVSEGLPAPGIHPLASVHETASLGEGVRVAAGAVIGPGVRLGAGCVID
                     AGVAVGANVELGAGCRLYPNATLYPGTVLGQRVVVHAGAVIGADGFGYAASPRGALKV
                     HHLGGVVLEDDVEIGANTCVDRGTLLNTRIGARTKIDNLCQVGHNVVIGSDTLIAGKA
                     GIAGSAAIGSGVIIGGDVAISDHVRVGDGARIAGRSGITKDVPPGATWAGFPARPHRE
                     FARELYLLGKLEELWRLARAGRRAGDMADGAAKTADGPAVSEAGANEVR"
     gene            complement(3315..>4347)
                     /locus_tag="PKN52_01640"
     CDS             complement(3315..>4347)
                     /locus_tag="PKN52_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98670.1"
                     /translation="TITAVEGDDVIAFGHPFLGMGAVSLPITPAYVTAIVPSSDVPFK
                     LANVGSSILGAIEQDRPGSISGRLGATPRTLHVDLSVVGVGQSANYSFAVPADDRLYP
                     TLVGTGVLSLLDRHLAATTAGYAEIAWEITLRGGERVNVIEQTNAPDDIAYRAAVLAL
                     TPLAILADNEFRESGVDHVSIAVRLEDRQRVATLEEAIAEESVVTAGENVVIHLRLQP
                     YRQNAIVRTISVPVPDDVEGELTVLIRGGDVPRDTGDAHVDEQEVDKPRSFPELLDAL
                     RQQVQASELIVEAVTESGEVRRLSRTPFAFVVDGNEDLTITVTPSDQDAGSGTPTDEG
                     APASGGTDD"
BASE COUNT          605 a         1526 c         1561 g          655 t
ORIGIN      
        1 acctaccagc gcgcgcgcct ccaccttgca gagcccgcgc ctgaacagga cgatggtgcc
       61 cgtgaggtgg agctggtcgc ccgggacgac cttgcgccgg aacttcgccg cttcgacgcc
      121 ggtcaggtag ccgaccttgg tgggtccgtc gcccgccggc cccccgccca ccaacccctc
      181 gcgcaccgcc acgccgacgg cggcggcctg ggcgagcgcc tccaggatga gcactcccgg
      241 catgaccggc tcctgaggga agtgcccgac gaagaacggc tcgttgttgc taacgttctt
      301 gaggcactcg aaggaatcgc cttccagctt cacgaaggcg tcgacgagaa ggaacgggta
      361 gcggtgcgga aggatgccgc ggatgtctac ggccatgtcc gtcagtgtac agggacgcca
      421 gcgccttctc gcatcgcggt gaggatgcga gtggcgagct cgagcgagcg catgtcgtcg
      481 gccaacgtac cgatcgggtc ggtcgtgcct ctgacgcggg cgaggaagtg ctccagctcc
      541 ttgcgcaagg ggttctcgtc gtgcacgagg tgccggtcga gcttcacgtg gccggagtgg
      601 gcggcgctgc cgggcgtgcg gaagaactgc acctcgctgt atggctcctt cgcgaaatcg
      661 acacggaccg tcttgtcgcg ctcggcgatg agcatggtgc gctccgggtc gggtgacacg
      721 cggctggcca ggaagcgcac gaggcaaccg ccctcgaacc tgatctgggc gatggccacg
      781 tcctcgagcg gcgagccgtt catcggcagg ccggaggcga ccacgtcgac gacgcggtcg
      841 ccgctcccca tgaggccgaa gacgatgtcg atgtcgtgga tcatgaggtc gagtacgacc
      901 ccgacgtctt tcacgcgctg ggaaggggag aggcggcgcg cctcgatcag gtagggctgc
      961 ctgacctcgc gcgccaacat ctgcaccgcg ctatagaacc tggtgatgtg gccgacctgc
     1021 agcaccagcc cctgcgcctc tgacaggcgg gcgagggcgg tggcctgggc gacgtccgtg
     1081 gctacaggct tctcgacgag gacgtggacg ccggcctcga gcaacgcctt ggtgaccggg
     1141 tagtggaggc tggtcggcac cgccacgctg gcggcgtcga ccacctcgtt ggcgagaagc
     1201 tcggcgagcg tcgcgtaccc cgcgcagccg taggccagct gcgcctcgcg ccgccgtgct
     1261 tcgtcgacct cgacgaccgc cacgatgcgg acacggggca gggtggcata gttggtggcg
     1321 tggtaacgcc ccatgacccc cgcgccgacc acggcgacgc gcagcagcgc gtcgggctgc
     1381 tggtcggtgg tgctcatgcc gtcgccgcca gcttcacggc cagtgccagc gcatgatgga
     1441 gggcgtgcga gccgcgttcg atggtgagcc gcgctcgcag tggacggccg aggagggcca
     1501 agtctccgag ggcgtcgagc gccttgtggc ggacaggttc gtctgggtgg cgtagaggac
     1561 gagtcgggcc gtccatcgcg aagacgataa catgctcctc gctcgctccc agcgccagtc
     1621 cgcgggcctt cagggcttcc caatcccgtt cgaagccgaa cgtgcgcgcg ctcaggagcc
     1681 ccgggtagtc ggccggaccc ccttcccacg cctgctcgcc gatcgcgggg tggtcgaacg
     1741 cgacccgata ggctagcgac tcctcgccgg gcgtcgcccg cgcccggccg ccgcggagcg
     1801 cgacctcgac ctcgcggccg agcgcgatgg ccgctggcgc ggtcggcgcc ggccccagct
     1861 tgtgcacggc ggcggcccac ggctccgccg agccgtcgag gatgggcaac tcgtcgcagg
     1921 tcgcctcgat gagcacgccc tcgaagaagc cggccacgcg cagcgcggcc agcaggtgct
     1981 cgacgagatg aacgttcgcg ccgtctgccg ccaggctcgt ggcgcgctca gcgccggcca
     2041 ggtggcccag gtcggcgggc acctcggttc cgccgcgcaa gaagcggatg ggtccgtcga
     2101 cgcggtgcaa cgtcacgctg ctcgtgagac cgcagtggat ggcgcgccct gtgaccgtca
     2161 ccatggccgg tcagcggacc tcgttcgcgc cggcctcgct cacggccggt ccgtcggcgg
     2221 tcttggcggc gccgtcggcc atgtcgccgg cgcgtctccc agcgcgcgcc aagcgccaca
     2281 gctcctcgag cttgccgagc aggtagagct cgcgcgcgaa ctcgcggtgc gggcgcgcgg
     2341 ggaagccggc ccacgtggcg cccggcggca cgtccttggt gatcccgctg cgcccggcga
     2401 tgcgcgcgcc gtccccgacc ctgacgtggt cgctgatcgc cacgtcgccg ccgatgatca
     2461 cgcccgagcc tatggcggcg ctcccggcta tgccggcctt gcctgctatg agcgtgtctg
     2521 agccgatgac gacgttgtga ccgacctggc agaggttgtc gatcttggtg cgcgctccga
     2581 tgcgcgtgtt caggagcgtg ccgcggtcta cgcaggtgtt ggcgccgatc tcgacatcgt
     2641 cctcgagcac gacgccgcca aggtggtgca ccttgagcgc gccgcgcggc gacgccgcgt
     2701 aaccgaagcc gtcggctccg atcacggcgc cggcgtggac cactacgcgc tgcccgagca
     2761 ccgtgccggg gtacagcgtc gcgttcgggt agagccggca gccagcgccc agctcgacgt
     2821 tggcgccgac ggccaccccg gcgtcgatga cgcagccggc gcccaaccgg acgccaggac
     2881 ctatcacggc gccggcggcc acgcggacgc cctcgccgag gctggcggtc tcgtggacgc
     2941 tcgccagcgg gtggatgccg ggcgcgggca ggccttccga cacgaagcag gcgctgagca
     3001 cggcgagggc gaggcgcgca tcgggcgcct gcgccaaccc ggtgccgtag tcggcggccc
     3061 aactcgtgcc gctcggcact accagtagcg ccggtcggct ggctggcggg ccggccgccg
     3121 cggcttcctt gctcatcacg caggcggcgt cggcgaggtc gacgccggcc tgccgcgtga
     3181 cggacgcgtc gacgaggcgc cggatcgacg ctccccccgt cggcccggtg gcgcccacgg
     3241 ccgcgccggt gccggtcaca ccggcgccgc tcacgagcgc ggcgacgagc tccccgtacg
     3301 ttcgggctcg cgtctcagtc atcggtgccg ccgctcgcgg gcgccccttc gtccgtgggt
     3361 gtccctgagc ccgcatcctg atcggacggg gtgaccgtga tggtgaggtc ctcgttgccg
     3421 tcgacgacga aggcgaacgg cgtgcgagag agccgcctga cctcgcctga ctctgtcacg
     3481 gcctccacga tgagctcgct tgcctggacc tgctgcctga gggcgtcaag gagctcgggg
     3541 aacgagcgtg gcttgtcgac ctcctgctcg tcgacgtggg cgtcgcccgt gtcgcgcggg
     3601 acgtcgccgc cccggatgag cacggtgagt tcgccctcca cgtcgtctgg gacagggacg
     3661 ctgatcgttc gcacgatggc gttctgccgg tacggctgca accgcaggtg gatcaccacg
     3721 ttctcgccgg ccgtcaccac gctctcctcg gcgatggcct cctcgagggt ggcgacgcgc
     3781 tgcctgtctt cgagccgcac ggctatcgac acgtgatcga ctccgctctc gcggaactcg
     3841 ttgtccgcca ggatcgccag cggcgtcagc gccaggaccg ccgcgcggta cgcgatgtcg
     3901 tccggggcgt tcgtctgctc gatgacgttg acccgctcgc cgcctctcag ggtgatctcc
     3961 caggcgatct cggcgtagcc cgccgtcgtg gctgcgagat gacggtcgag tagcgacagc
     4021 acgcccgtgc cgacgagcgt cggatagagc cggtcgtcgg ccggaaccgc gaacgagtag
     4081 ttggcgctct gcccgacacc cacgacgctc agatctacat ggagggtgcg aggggtcgcg
     4141 ccgagcctgc ccgagatcga gcctggccgg tcctgttcga tggccccgag gatgctggag
     4201 ccgacgttgg ccagcttgaa tggcacgtcc gaggacggga ctatggccgt cacgtatgcg
     4261 ggcgttatgg gtagcgagac ggcgcccatc cccaggaaag ggtggccgaa cgctatgacg
     4321 tcatcgcctt ccaccgccgt gatcgtg
//
LOCUS       DAOQYE010000052         4257 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_21183_length_4257_cov_3.644455, whole genome shotgun sequence.
ACCESSION   DAOQYE010000052 DAOQYE010000000
VERSION     DAOQYE010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 4257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4257
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21183_length_4257_cov_3.644455"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(419..1768)
                     /locus_tag="PKN52_01645"
     CDS             complement(419..1768)
                     /locus_tag="PKN52_01645"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNQ98671.1"
                     /translation="MRLLARLRRPNLHRRLAVALLAQTVVVLLVTALALFLVLRQFLE
                     VGEAQRLERLVGQVSVHVASDKHDVLEFDHDFPDDVHVRLLHQGVVLAATPGFPAIPV
                     DSSLGRSRSAQHQVLTRELREDGVRYTLQLAGDPTGTQLALRAYVMALAVIVPVAAVL
                     VALLSNLLAANMLTPVARLERAAAAISSSRELRTPIQGVDATDELGRLANTLQAAFGR
                     LADGMDREVAFLRAAAHDLRSPLTALKTRVEGVLANRRDASAYRAALVEIGLDVDGMV
                     RLVNHLLMLANDGAPNDLRRLDLVSVAGEAVDSARAARPGVFLTTSFAPDTPEVAGDA
                     TLLAQLLGNLLDNAAHHGGGAPTHVSTWRSPEGGAVLCVSDEGPGIDEASLPRAAEPF
                     FRTDSARTRAGRGGPTGSGLGLAIVKRIVEWHDARWELNSAPGSGLVVTVRFPAPLG"
     gene            complement(1771..2430)
                     /locus_tag="PKN52_01650"
     CDS             complement(1771..2430)
                     /locus_tag="PKN52_01650"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNQ98672.1"
                     /translation="MRLLVIEDDPRIGAPLVEALEEGGHRVAWVQDGPGGLAEARAGE
                     FDALILDVMLPGLDGFDVARTLRSEGVASPILFLSARGELTDRVTGLDLGGDAYLVKP
                     FALAELKATLRALVRRGNELAPERIGFGSGRGELLAGERRVLWDGHEVALTGREYDVL
                     EALLRAPERWFTRQELLDRVWGPDFFGEPRVVDVYVRYLRQKLADSVIESMRGRGYRA
                     S"
     gene            complement(2405..3067)
                     /locus_tag="PKN52_01655"
     CDS             complement(2405..3067)
                     /locus_tag="PKN52_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98673.1"
                     /translation="MTTTTSASRLNRYLVWLPLAALSLYWLLAYAGLAPGEWTWGFLR
                     LTGVIGYALLALSVASGALLAARFAPPAWLAKPLQYGWHGLTAGSGLALVGIHVALSL
                     VSAHYPQSLDGVLVPGLATFSPFAMGLGTIGTYLLLAPYLSFAGRRVLSGRWVKVFHL
                     FAYPGFAAGTLHGMLIGSDRLAWLYLAGLALVAFTVSVRLAEGRCPRLEAGAHEAAGH
                     RG"
     gene            complement(3090..3881)
                     /locus_tag="PKN52_01660"
     CDS             complement(3090..3881)
                     /locus_tag="PKN52_01660"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HNQ98674.1"
                     /translation="MRVEFSGMGTTIAVQAPEARRLATELRRLEALLTRFDPAGGVAG
                     LNRTGRLRDPAPELLAAVRWAVEACHLTDGLVTPLVGRSLEWHGYSGSWSEGVDWQAR
                     EGKPPPVPPIEQLVVSADEIVLPPGAALDLGGTAKSWAVQRVATGLDRVVIDAGGDVL
                     IRSAAPSRVNVMGAAEPWHLELPPGAWAVATSSVARRAWRGGHHLIDPRTGRPVRGRW
                     TQATVVGRSLALAELATKLLLLGVPVPETLQVEQAWVVDRDGTLR"
     gene            complement(3959..>4257)
                     /locus_tag="PKN52_01665"
     CDS             complement(3959..>4257)
                     /locus_tag="PKN52_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98675.1"
                     /translation="GSGCGKPARRRPVAAATLRFAMDDRDGAVAARLTSGERPPLAAA
                     GTSVRVVVVDPSGRVIGTYDGSDLAPHATVAPAATGATLRRGESRSAVAGTRAS"
BASE COUNT          685 a         1521 c         1465 g          586 t
ORIGIN      
        1 tgtcgagcgc tacgtctggg tgggtcttct ggaactcggg gaaggccgtg tcgcggaacc
       61 agttgagcgt gtcgtccgtc aggccggtga tgtagatctc gagcgagcgc tgcgcgttga
      121 cggagccgag tagcagcccg gccaacgcga tcagcatcag gaacttcttc ttcatgcttg
      181 cctccacggt cttgatcagc gtacctaggt tcttgctcgt tatttggcac gcccgttcgt
      241 cctcttgagc cgctcggcct tccgagcagg ccactgtcgc aagtcgctgt tcctcgtcac
      301 ctccctaggc ggggcctcac gagaccggtc ggttagtaag gccaggttac ttactatgct
      361 tgggtcaagg ttaggggggc gacgggcgat tgtcaagtga gttcaagggg ggcgctcctc
      421 aaccgagcgg cgccgggaat ctcaccgtca cgacgagtcc cgaacctggg gcgctgttca
      481 gctcccaccg cgcgtcgtgc cactccacga tgcgcttcac tatcgcgagc cccaagccgc
      541 ttcccgtagg gccgcctctc ccggcgcgcg tgcgcgccga gtcggtccgg aagaagggtt
      601 cggccgccct gggcagactg gcctcgtcga tgccaggacc ctcgtcgctt acgcacagca
      661 cagctccacc ctcaggggac cgccaggtac tcacgtgggt cggcgcgccg ccgccgtggt
      721 gagccgcgtt gtcaagtagg ttgccgagta gttgggccag tagcgtcgcg tcgcctgcaa
      781 cctccggggt atccggggcg aacgaggtcg ttaggaacac gccaggacgg gccgcgcgag
      841 ccgagtcgac tgcctctccc gccacgctca cgaggtccag cctccggagg tcgttcggcg
      901 ccccgtcgtt ggccagcatg agcaagtggt tgacgagtcg caccatcccg tcgacatcga
      961 gcccgatctc gaccagggct gcgcgatagg cgctcgcgtc gcgcctgttc gccaacacgc
     1021 cttctaccct cgtcttgagc gcggtgaggg gggaccggag gtcgtgcgcc gcggcccgca
     1081 agaaggcgac ctcccggtcc atcccgtccg ccaagcgccc gaatgccgcc tgcaacgtgt
     1141 tggcgagtcg ccccagctcg tcggtagcgt ctacaccctg gatcggcgtg cgcagctcac
     1201 gggagctgga gatggccgct gcggcgcgct cgaggcgggc aacgggagtc agcatgttgg
     1261 cggcaagtag gttgctgagc agcgccacga ggacggcggc gaccggcacg atgacggcca
     1321 gcgccatcac gtaagcgcgg agcgccagct gggtacctgt ggggtcgccc gccagctgca
     1381 gcgtgtagcg cacgccgtcc tcgcgcaact cgcgcgtcag cacctggtgt tgcgccgacc
     1441 tcgagcgccc gaggctcgaa tcgacgggga tcgccgggaa gccaggcgtc gccgcgagca
     1501 ccacgccctg gtgcagcagg cgcacatgta cgtcatccgg aaagtcgtgg tcgaactcga
     1561 gtacgtcatg tttatcgctg gccacgtgga cactcacctg accaacgagg cgctcgaggc
     1621 gctgtgcctc acccacctcg aggaactggc gcagcacgag gaacagggcc aaggccgtga
     1681 cgaggagcac cacgaccgtc tgcgccagca gggcgaccgc caggcggcgg tggaggttcg
     1741 gacgccgaag ccgtgcgagg aggcgcaccc tcatgaggct cggtaccccc tgccccgcat
     1801 actctcgatc accgagtccg ccagcttctg ccgcaagtac ctgacgtaca cgtccaccac
     1861 ccggggctcc ccgaagaagt cggggcccca gacgcggtcg agcagctcct ggcgcgtgaa
     1921 ccaccgctcg ggcgcgcgca gcagagcctc gagcacgtcg tactcacgcc cggtgagcgc
     1981 cacctcgtga ccgtcccaca gaacgcggcg ctcaccggcg aggagttcgc cgcgcccgct
     2041 accgaacccg atgcgctcag gagccagctc gttgccgcgc cgcaccaatg cccgtagcgt
     2101 cgccttcagc tcggccaggg cgaacggctt gaccaggtaa gcgtcaccgc cgaggtcgag
     2161 gcccgtaacc cggtcggtca gctcgccccg cgcgctcaag aacaggatcg gggaggcgac
     2221 gccttcactg cgcaaggtcc gcgccacgtc gaaaccgtcc aggcccggca gcatcacgtc
     2281 caggatcaac gcgtcgaact cccccgcgcg ggcctccgcg agcccaccag ggccgtcttg
     2341 gacccatgcg actcggtgac caccctcctc caaggcctcg acgagggggg cgcctatacg
     2401 cgggtcatcc tcgatgacca gcagcctcat gcgctccggc ctccagcctc gggcaccgcc
     2461 cctccgcgag ccgcaccgag acggtgaacg caaccaacgc cagccctgca aggtagagcc
     2521 acgcgagtcg gtcgctgcct atcagcatcc catgcaacgt gccagcagcg aacccggggt
     2581 aggcgaacag gtggaacacc ttcacccagc gaccgctcaa gaccctgcgc ccggcgaagc
     2641 tcaggtacgg cgcgaggagc aagtaagtgc cgatcgtgcc gagacccatg gcgaagggcg
     2701 agaacgtggc gagccccggc acgagcacac cgtccagcga ctgcgggtag tgcgcgctca
     2761 cgagcgacag cgccacgtgg atgccgacca gtgccagacc gctcccggcc gtcagaccgt
     2821 gccacccgta ctgcagcggc ttggccagcc aggcgggtgg cgcgaaacgc gcggccagga
     2881 gagcgccgct cgccacgctc agggccagga gcgcgtagcc gatcacgccc gtgaggcgga
     2941 ggaaccccca ggtccactcg ccgggcgcca gtccggcgta ggccagaagc cagtagaggc
     3001 tcagggccgc gagcggcagc cacaccaggt aacggttcag gcgggaggcg ctggtggtag
     3061 tggtcacgag aacggtccag tcacgctcgt caccggaggg tgccgtcccg gtcgacgacc
     3121 caggcctgct cgacctggag cgtctccggg acgggcacgc ccaggagcag cagtttcgtg
     3181 gccagctcgg ccagggcgag cgagcggccg accaccgtgg cctgcgtcca ccgtccacgc
     3241 acgggcctgc cggtgcgcgg gtcgatgagg tgatgaccac cacgccaagc gcggcgcgcc
     3301 acactcgagg tggctaccgc ccaggcgccg gggggcagct cgaggtgcca aggctcggcg
     3361 gcgcccatga cgttcactcg gctcggcgcg gcggagcgga tgagtacgtc gccgccggcg
     3421 tcgatcacga cccggtcgag tcctgtggcc acgcgctgca cggcccacga cttggcggtg
     3481 ccgcccaagt cgagggccgc gcccggcggc aggacgatct cgtcggcaga caccacgagc
     3541 tgctcgatag gcggcacggg tggtggcttg ccctcccgag cctgccagtc gacgccctcg
     3601 ctccaactcc cagagtagcc gtgccactcg agggagcgcc ccaccagcgg cgtgaccagg
     3661 ccgtccgtca agtggcacgc ctccacggcc cagcggacgg ccgccaggag ttcaggcgcc
     3721 gggtcgcgca gccgcccggt gcggttcagg cccgcgacac ccccggcggg gtcgaaacgc
     3781 gtgagtagcg cctcgaggcg gcgcagctcg gtcgccaggc gccgcgcttc gggtgcttgc
     3841 accgcgatcg tcgtgcccat gccgctgaac tcgacgcgca cggtagacgg cgggggggcg
     3901 gggcgctggc ggccgccggt gtgacttggg cgtgggccga gcgaggggaa gtgcgctgtc
     3961 atgacgctcg cgtgccggct acggcggaac gcgactcacc gcggcgcaac gttgcccctg
     4021 tagcggccgg cgcgacggtt gcgtgagggg cgaggtccga gccgtcgtag gtgccgatca
     4081 cacggccgct cgggtccacg accacgacgc gcacgctcgt cccggcagcc gcgagcgggg
     4141 gacgttcccc gctcgtcaag cgcgcggcta ccgccccgtc gcggtcgtcc atcgcgaagc
     4201 gcagcgtcgc cgcggcgacc ggccgccgcc ttgccggctt gccacagccc gagcccg
//
LOCUS       DAOQYE010000053         4246 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_21268_length_4246_cov_2.816512, whole genome shotgun sequence.
ACCESSION   DAOQYE010000053 DAOQYE010000000
VERSION     DAOQYE010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 4246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4246
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21268_length_4246_cov_2.816512"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..976
                     /locus_tag="PKN52_01670"
     CDS             <1..976
                     /locus_tag="PKN52_01670"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNQ98676.1"
                     /translation="DRLDDIGRRHHMLARRFAPEMEPAPGAALGSVRFGVEANLARGA
                     RPGDLVLCRVNAPLLRSAFALVEAGLPARVLGEEVLSSTLELAQTVFAGAERLPSTAV
                     ALVERHAREENLRLEHELVTSPTLPERLTASADSHRALALLLRAAQQRRSEWSLRALL
                     RPPVLSFAELEAVARELFEPEHLRDCVLLSTIHKAKGREAERVLLLRPEGLGIAGNGA
                     DAHAANDEAAHGPDDSANDRAADEAEANVLFVALTRAKREYVFLEGRRGAVAERLRSH
                     AKTPPSSQLERRWNGVLRLAVAMARRGDSPAVKPPSAWVAGRGRSSGS"
     gene            complement(924..1232)
                     /locus_tag="PKN52_01675"
     CDS             complement(924..1232)
                     /locus_tag="PKN52_01675"
                     /inference="COORDINATES: protein motif:HMM:NF013379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HNQ98677.1"
                     /translation="MKPQSDAARPGAAGTVHKLDNRGSPCSLGLLRVKQRLADMPLGD
                     VLEVVTRDRFAPYEVPAWVDRQGLQLVSLVRSGFWLFATTTFTIRKTVLVLPPRQKVA
                     "
     gene            complement(1273..2217)
                     /locus_tag="PKN52_01680"
     CDS             complement(1273..2217)
                     /locus_tag="PKN52_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98678.1"
                     /translation="MADSTAPFIPAVAGDTAARLEVGVEVRTGTPFLPRAAFFLISVA
                     SFVGVYFTGRLHGLEGVGLVWRWLQFWGLALTSGMLAWRLLYLRRSEAGLHGERVLAL
                     FDDLLARVRVLRRFLVPLLLVGAPAPWFVPYLTAWQAAALTCVSLLAALALLGAHEHR
                     VAASTAFLSGLAGLVVWGWADSSGLVSLAAVRTVHLWAFAAWLGGALFNLGAAVPAGR
                     RNANLDAVVAGARQLERFRWVVRVALPTVILTGVWMALRYGGLTSSFWLGGLGLLIPL
                     KLVLILALVVIFITCPLYRACSPVRGVCNLDDLGASGD"
     gene            2306..2611
                     /locus_tag="PKN52_01685"
     CDS             2306..2611
                     /locus_tag="PKN52_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98679.1"
                     /translation="MPLHGEFKQPGGKLVVVDLELRDGYLAEVHVSGDFFLEPPEALD
                     DIVAALTGAPVDASEDDLATRIDEALAPDAMLIGFDARGVGKAVRRAVTDGSPRERL"
     gene            2608..3411
                     /locus_tag="PKN52_01690"
     CDS             2608..3411
                     /locus_tag="PKN52_01690"
                     /inference="COORDINATES: protein motif:HMM:NF015084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoate A/B protein ligase family
                     protein"
                     /protein_id="HNQ98680.1"
                     /translation="MSTDTDRFARFRPRWNEFDWQLIRTGAQAPELHMALDEVLTYEV
                     GSGHRKPTLRIWEWASNAVILGRFQSVRNEVDPDGAARHDIAVMRRITGGGAMFIEPQ
                     NAITYSIYAPDSLVRGLSFIDSYAFMDEWVIQGLRSLGVEAEYVPINDISSTAGKIGG
                     AAQTRRGGAVLHHVTMAYDMDPAKMLEVLRIGREKLSDKGSVSANKRVAPLRQQTQLP
                     RQVVVDRLVETFADLRGLTGGELTTAELTRAEALAKEKFLSDEWRYLLP"
     gene            3563..>4246
                     /locus_tag="PKN52_01695"
     CDS             3563..>4246
                     /locus_tag="PKN52_01695"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNQ98681.1"
                     /translation="MRFRSVTRALVAVLLLFVGSAFAQDTFRIAIGVDADSLDPAQGT
                     TTTIDNIIDYMAQTLTGIDETGTLVPVLATAWESSADGLQLDMTLREGVVFHDGSAFT
                     ADTVVWNFNRLLDPEVNVPRRGPYAVIDSVEALDEHHVRFNLKQPAPYLTGALTQSTA
                     AMISPASIDLEGNSYANITHPVGTGPYTFTSRAFGDRIEVTRFDGYWGELPYYEHVVF
                     HVVPEATTRE"
BASE COUNT          737 a         1465 c         1430 g          614 t
ORIGIN      
        1 cgatcggctt gatgacatag ggcggcgcca tcacatgctc gcgcgccgct tcgcaccgga
       61 gatggagcca gctccgggcg ccgctttggg ctcggtgcgg ttcggtgtcg aggccaacct
      121 ggcgcgcggc gcgcggcctg gcgacctggt gctctgccgc gtcaacgcgc cgctgctgcg
      181 tagcgccttc gcgctcgtcg aggccgggct gcctgcgcgg gtcctcggcg aagaggtctt
      241 gagcagcacg ttggagttgg cgcagaccgt cttcgccggc gctgagcgcc tgccgtcgac
      301 cgccgttgcg ctggtagaac ggcatgcccg cgaggagaac ctccgcctcg agcatgaact
      361 cgtcaccagt cccacactgc cggagcgcct gaccgccagc gcggactcgc accgcgccct
      421 cgcgctactc ctgcgtgcgg cgcagcaacg ccgctcggag tggagcctca gggcactcct
      481 gcgtccgccc gtcctctctt tcgcggagct cgaggcagtg gcacgcgagc tgttcgagcc
      541 ggagcacctc cgcgactgcg tcctcctctc gaccatccat aaagctaagg ggcgggaagc
      601 ggagcgagtg ctcctcctgc ggcccgaagg actcggcatc gcggggaacg gcgcagacgc
      661 ccatgccgcc aacgacgagg ccgctcacgg gccagacgac agtgccaacg atagggccgc
      721 cgacgaggcc gaggccaacg tcctcttcgt ggccctgact cgcgccaagc gcgagtacgt
      781 gttcctggag gggcgtcgcg gggcggtcgc agagcggctg aggagtcacg ccaagactcc
      841 tccgtccagc caactggaac gccggtggaa cggcgtgctg aggttggccg tggcgatggc
      901 gcgccgcggc gacagcccgg ccgtcaagcc accttctgcc tgggtggcag gacgaggacg
      961 gtcttccgga tcgtgaaggt ggtggtcgcg aaaagccaga agccggagcg cacgagcgag
     1021 accagctgca acccctgccg gtcaacccag gcgggcacct cgtaaggtgc gaaacggtcg
     1081 cgcgtcacga cctcgaggac gtcgcccagc ggcatgtcgg ccagacgctg cttcacccgc
     1141 aggagcccaa gcgagcaggg cgagccgcgg ttgtcgagtt tatggacagt gcccgccgcg
     1201 cccggccgcg ccgcatcgct ctgcggcttc acgtgtggct tctcgtgcgc ttcccagcca
     1261 tctcgtgcgc cttcaatcgc cactcgctcc gaggtcgtcg aggttgcaga ccccacggac
     1321 gggcgagcag gcacggtaga gcgggcaggt gatgaagatg acgacaagag cgaggatgag
     1381 tacgagcttg aggggtatga gaagccctag tcctccgagc cagaacgagc tcgtgagacc
     1441 cccgtacctc agcgccatcc ataccccggt gaggatgacg gtcggcaagg ctacccgcac
     1501 gacccacctg aagcgctcca actggcgcgc gccggccacc acggcgtcga ggttggcgtt
     1561 gcgccgcccc gccggcacgg ccgcgccgag gttgaagagc gctcccccga gccaggcggc
     1621 gaaagcccag aggtgcacgg tcctgaccgc agcgagcgag accaagccag agctgtccgc
     1681 ccagccccac acgacgaggc cggccaaccc ggacaggaac gcggtggacg cggccacgcg
     1741 gtgctcatgc gcgccgagga gggcaagcgc cgccagcagg ctcacgcacg tcagcgccgc
     1801 agcctgccac gcggtcaggt aggggacgaa ccagggcgcc ggcgcgccaa ccaggaggag
     1861 cgggaccagg aagcggcgca gcacgcgcac acgcgccagg aggtcgtcga agagtgccag
     1921 aacgcgttcg ccgtgcaggc cggcctcaga gcgccgcaag tacaggaggc gccaggcgag
     1981 catgcccgac gtgagcgcca acccccagaa ctggagccac cgccatacga gtccgacgcc
     2041 ctcgagcccg tggagcctgc cggtgaagta cacgcctacg aaggaggcca cggagatgag
     2101 gaagaacgct gctctcggga ggaaaggcgt cccggtcctg acctcgacgc caacttcgag
     2161 gcgcgccgcc gtgtcgccgg cgaccgcggg gatgaagggt gcggttgagt ctgccatggt
     2221 cagccgcagc gtagtcctga gtcgttcact cggctttgac cctggtcaac ccgacggcag
     2281 acgcggtgac ggtaacatcg tccccatgcc ccttcacggc gagttcaagc aacccggcgg
     2341 caagctggtc gtggtcgacc tggagctgcg ggacgggtac ctggccgaag tccacgtcag
     2401 tggcgacttc ttcctcgagc cccccgaggc gctcgacgac atcgtggccg ccttgacggg
     2461 cgctcccgtc gacgcgagcg aggacgacct ggcgacccgc atagacgagg cgctcgcgcc
     2521 tgacgcgatg ctgatcggct tcgacgcgcg cggggtcggc aaggccgtac gccgcgccgt
     2581 gacggacggc agcccgaggg agcgcctgtg agcaccgata ctgaccgctt cgcccgcttc
     2641 cgcccacgtt ggaacgagtt cgactggcag ctcatccgca cgggtgcgca ggcgccggag
     2701 ctccacatgg cgctcgacga ggtgctcacc tacgaggtcg gctcggggca taggaagccg
     2761 acgctccgga tctgggagtg ggcttccaac gccgtcatcc tcggacgctt ccaatccgtc
     2821 cgcaacgagg tcgacccgga tggcgcggcc aggcacgaca tagcggtcat gcggcgcatc
     2881 accggcggcg gggccatgtt catcgagccg cagaacgcca tcacgtactc gatctacgcg
     2941 cccgactcgc tcgtgagggg cctgtcgttc atcgactcat acgccttcat ggacgagtgg
     3001 gtcatacagg gtctgcgctc gctcggcgtg gaggccgagt acgtgcccat caacgacatc
     3061 tcctccaccg ccggcaagat cggcggcgcg gcccagacgc ggcggggcgg tgcggtgctg
     3121 caccacgtga ccatggccta cgacatggac ccggccaaga tgctcgaggt cctgcgcatc
     3181 ggccgcgaga agctatcgga caagggcagc gtgagcgcca acaagcgcgt ggcgccgctg
     3241 cgccagcaga cgcagctgcc ccgtcaggtc gtggtagacc ggctcgtcga gacgttcgcg
     3301 gacctccggg gactgactgg cggcgagctc acgaccgccg agctcaccag ggcggaggcg
     3361 ctcgccaagg agaagttcct gtcagacgag tggcggtacc tgttgccctg acgcttacga
     3421 gcgccgatca tccacgggtg tcgacgccgt cgcggcatcc ggttctcgtg gcgatcgctc
     3481 cccgaggcac cacctcccgg cccgatacct gatatgacta tctagaacaa aagattgtcg
     3541 tcatagctct gggaggtgcc acatgcgttt tcgttccgtt acgagggctc tggtagctgt
     3601 tctgttgttg ttcgtcggct cggcgttcgc gcaggacacg ttccgcatcg ccatcggcgt
     3661 ggacgccgac tcactagatc cggcgcaggg caccacgacg acgatcgaca acatcatcga
     3721 ctacatggcc caaaccctca ctggcatcga cgagacgggc acgttggtgc cggtgctcgc
     3781 caccgcttgg gaatcgtcgg ccgacgggct gcaactcgat atgacgctgc gcgaaggggt
     3841 cgtgttccac gacggctcgg cgttcacggc tgacacggtc gtgtggaact tcaaccgcct
     3901 tctcgacccc gaagtcaacg tgccgaggcg cgggccgtac gcggtcatcg acagcgtcga
     3961 ggcgctcgac gagcaccacg tgcgtttcaa cctgaagcag ccggccccgt acctcactgg
     4021 cgccctgacg cagagcacgg ccgccatgat ctcaccggcc tccatcgacc tcgaaggcaa
     4081 ctcgtacgcc aacatcaccc accccgtagg gaccggaccg tacacgttca cgtcgcgagc
     4141 gttcggcgac cgcatcgagg tcacgcgttt cgacggctac tggggcgagc tcccctatta
     4201 cgagcacgtc gtgttccacg tcgttcccga ggccaccacg cgcgag
//
LOCUS       DAOQYE010000054         4227 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_21406_length_4227_cov_2.737296, whole genome shotgun sequence.
ACCESSION   DAOQYE010000054 DAOQYE010000000
VERSION     DAOQYE010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 4227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4227
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21406_length_4227_cov_2.737296"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..398)
                     /locus_tag="PKN52_01700"
     CDS             complement(84..398)
                     /locus_tag="PKN52_01700"
                     /inference="COORDINATES: protein motif:HMM:NF013089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family transporter"
                     /protein_id="HNQ98682.1"
                     /translation="MAWIVLIVSGLFEAVWATALGRSAGLTRPVPSLVFLAALAVSMG
                     GLAYAMRSLPTGTSYAVWVGVGAGVAVAFAMLSGNEPFSWAKAALLAGLVACIAGLRL
                     VA"
     regulatory      complement(410..476)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01763"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="guanidine-III (ykkC-III) riboswitch; guanidine
                     riboswitches control genes whose products are involved in
                     modification or pumping out guanidine as a toxic compound
                     from bacteria; this is a third class of guanidine
                     riboswitches based on ykkC-III RNA motif; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="guanidine"
                     /db_xref="RFAM:RF01763"
     gene            547..3306
                     /locus_tag="PKN52_01705"
     CDS             547..3306
                     /locus_tag="PKN52_01705"
                     /inference="COORDINATES: protein motif:HMM:NF015649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HNQ98683.1"
                     /translation="MDPAPARAAPPEVERIRLLSRLPDAPGFVVHLRAPYGFGKTWLA
                     RAHLRRLAADRWRVLEVSIDGRSVGPALAAALGLPSRTAPATLREALWATGTVLFLDD
                     LGATDERDPTLERLLKEPQGVLLLASRGQFVPPEFVRLGAEGRLSTLDATDLAFDEVE
                     ATALFGGSGTLAADALHRYGGWPLLLAHSASTGAAPDAALMTAAAENALDRSAWEALL
                     LLAAVPDISEVLLGEGGADLLASGLASTDARGVRPLRMLAAACLTARRSEVLEVVTRY
                     RGGLGALQLATAFERLGEPAALARVIDTTDAPLAREDPQAVLRWYGLIGDATEGVARA
                     RRRLLVGNALAAVGRASEGGSMLEALAEEATLPPDLRLQALGDAIYALAGDPDCHARA
                     RELLEASADLAASASAERRGLFFSTASVLDFRAGDLAAAKRMVERALGELPADNPLRF
                     GPLINLAVLSWNSEGDLEGRIQLQESCLELCRAHYPDHVVGVCRDLAHLSLYLGRTER
                     ARAYLAEARRFGASRPLFLLDVEAMSAALDKDLPRLREVASVAVRCGDAGMADAVTWR
                     YLTALRHAAGAAGAAGTARVVEAETDLRPLGPFGTVALALALLDTGEPAGAEVLLERV
                     RPEDDEREFRLEWAAARYQVTRDEAELDRLIGLTKSGAAILPALVPLGALPRHRPELA
                     RSYPLPNVMRSGWRQAAEARADELPRLEVEYLGSTRVTVLGEVVEVAGRSRDLFAMLL
                     LRVGRREIGATLWPESNRTKVRNNLSVQYSLLRHVLEPWGTRRYLPDDALEHTSADVW
                     RLEQALRARDADAALTLYRGPFAPLVDVPAVVDARTHLERAMVDLLVERAATAHADRA
                     VHYLRRALEIDPLDEGAVQALLGRLVAAGRAGEAEQRYRRFEADLRRELGTEPRAETR
                     AVLAR"
     gene            complement(3373..>4227)
                     /locus_tag="PKN52_01710"
     CDS             complement(3373..>4227)
                     /locus_tag="PKN52_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98684.1"
                     /translation="RAFARAVGSAGGILKIVALVLTGADVVAVVSDMAGNPPGAAWEV
                     IRTEVSAKLTPSEGQVGKYAPSTDFKVVIDAPLDGGSYVYRWSTSGEYGYLYDYLGQE
                     GQSLETTRSQVLYVANAPNAITDDLRDTIAVEVYKDDGSGKVAPGAALVAQATATVIG
                     KEKQDLFAGRWFYHAASYADGAGNPRVCQVIYLGIKLVPGATTYEATGTGFYDPYYYG
                     STLAVGYRTGQTVPPPTDDNPCPYERLQDGEYQKLLTGGWGPAGSEDPGLFNGRFQGM
                     VVEVVVTY"
BASE COUNT          609 a         1513 c         1485 g          620 t
ORIGIN      
        1 gtcgtccccg tgatcctcga cgcgcgcgtg aagcggcggc aacgcctgcg ggagctcgac
       61 gactagggac gcgcgggacc ggttcaggcg acgaggcgca gccccgcgat gcaggcgacg
      121 agcccggcga ggagggcggc cttggcccac gagaacggct cgttgccgct cagcatcgcg
      181 aacgccaccg ccacgccggc gcctacgccc acccacacgg cgtaggaggt gccggtgggc
      241 agcgaccgca tggcgtaggc taggcccccc atgctcacgg ccagggcggc gaggaagacg
      301 aggcttggta cgggccgcgt gaggccggcc gagcggccga gggcggtggc ccagacggcc
      361 tcgaacaacc cggagacgat gaggacgatc cacgccatga ggcacctcca tggcgacgtc
      421 ttgtcgttac cgggtacgtc gctctcgtcc gggaccgcga ccgtcgcacg gcgtggtcga
      481 ggtcagacac tagcacgcat ccgcaacggg gtccggcgaa gccggtgcta ccatcggggc
      541 cacacgatgg atcctgctcc cgcgcgcgca gctccccctg aagtcgagcg catacggttg
      601 ctgtcccgcc tacccgacgc gcccggcttc gtcgtgcacc tccgcgctcc ctacggtttc
      661 ggcaagacat ggctggcacg agcgcacttg cgccgcctcg cggctgatcg ttggcgcgtc
      721 ctggaggtat cgatagacgg ccgcagcgta ggccccgcgc tggcggccgc gctaggtctc
      781 ccgagccgta ccgcccccgc gacgctgcga gaggccctct gggcgaccgg aacggtcctc
      841 ttcctcgacg accttggcgc cacggacgag cgcgacccga cgctcgagcg gctcctcaag
      901 gagccccaag gcgtcttgct cctggcgtca cgaggccagt tcgttccgcc cgagttcgtc
      961 cggttgggtg ccgagggtcg cctgtcgacc ctggacgcga cggacctggc cttcgacgag
     1021 gtggaagcca cagcgctgtt cggcggctct ggaacgctcg cggccgacgc ccttcaccgc
     1081 tacggcggct ggcccctgct cctggcccac tcggcgagca ccggcgccgc gcccgacgca
     1141 gccctgatga ccgccgccgc ggagaacgct ctcgaccgga gcgcgtggga agcgctcctc
     1201 cttctggccg ccgtgcccga cataagcgag gtcctgctcg gtgagggggg tgcggacctg
     1261 ctcgccagcg ggttggcttc gaccgacgcg cgcggcgtgc ggcctctccg catgctggct
     1321 gcggcctgcc tgaccgcccg ccggagcgag gtgctcgagg tcgtcaccag gtaccgcggc
     1381 gggctaggcg ctttgcaact cgcgaccgcc ttcgagcgtc tcggggagcc agccgcgttg
     1441 gcgcgagtga tcgacacgac cgacgcccca ctggcgcgtg aggacccgca ggccgtgctc
     1501 aggtggtacg gactcatcgg cgacgcgacc gaaggggtgg cgcgcgcccg caggcggcta
     1561 ctcgtcggca acgccctggc tgcagtgggg cgagcgagcg aagggggctc gatgctcgag
     1621 gcgctcgccg aggaagccac cctgcctccc gacctgcgcc tgcaggcgtt gggcgacgcg
     1681 atctacgcac tggccggcga tcctgactgc cacgctaggg cgagggagct cctcgaagca
     1741 agcgccgact tggcggcgag cgcgtcggcg gaacggcgcg gcctcttctt cagcacggcg
     1801 tccgtcctcg acttccgcgc aggcgatctg gccgcagcca agcgcatggt cgagcgcgcc
     1861 ctcggagagt tgccggccga caacccccta cgcttcgggc cgctcatcaa cctcgccgtc
     1921 ctctcctgga actcggaggg cgacctggag ggccgcatcc agcttcagga aagctgcctg
     1981 gaactctgcc gggcgcatta ccccgaccat gtcgtcgggg tatgccgtga cctggcgcac
     2041 ctctctctat accttggccg aaccgagcgc gctcgcgcgt acctggcgga ggcacgccgc
     2101 ttcggggcgt cccggccgct cttcctcctc gacgttgaag ccatgtccgc agcgctcgac
     2161 aaggacttgc cccgcttacg cgaggtagcc tcggtcgctg tcagatgtgg cgacgcgggc
     2221 atggccgatg ccgtgacctg gcgctacctg accgcactcc gacatgcggc tggcgccgcg
     2281 ggcgcggcgg gcacggcgcg cgtcgtcgag gcggagaccg acctccggcc cctcggaccc
     2341 ttcggtacgg tggccttggc gctcgcgctg ctggacaccg gggaaccggc gggggcggag
     2401 gtgctccttg aacgcgtgcg gcctgaggat gacgagcgcg agttccggct cgagtgggcc
     2461 gctgctcggt accaagtgac acgcgacgaa gccgagctgg accgtctgat cggcctcacg
     2521 aagagcggcg cagccatcct gcccgcccta gtgccgctgg gggcgctccc acgccaccgc
     2581 cccgaactgg cgcgctcgta ccctctgccg aacgtgatgc gctctggctg gcgccaagcc
     2641 gctgaggcgc gtgcggacga gttgccgcgg ctcgaggtcg agtacctcgg cagcacgaga
     2701 gtgacggtac tcggcgaggt cgtggaggtc gccggacgat cgagggatct gttcgccatg
     2761 ctgctcctgc gcgtcggccg ccgcgagatc ggggccaccc tgtggcccga gtcgaaccgg
     2821 accaaggtca ggaacaacct ctcggtgcag tacagcctct tgcgccacgt cctcgagccg
     2881 tggggcacgc gtcgctactt gccggacgac gccctggagc acacgagcgc agacgtgtgg
     2941 cgactggagc aggcgctgcg tgcccgcgac gcggacgccg cgctgaccct gtaccgcggc
     3001 ccgttcgctc cactcgtcga cgtccctgcc gtcgtcgacg ccaggaccca cctcgagcgg
     3061 gcgatggtcg acctgctggt tgagcgcgct gccacggctc acgccgaccg ggccgtccat
     3121 tacctgcgcc gcgcactcga gatcgaccca ctcgacgaag gggcagtgca agcgctcctc
     3181 ggacggctgg tggccgccgg ccgcgcgggc gaggccgagc agcgctaccg gcgcttcgag
     3241 gccgacctcc gcagggagct cggtacggag ccgagggcgg agacgcgagc cgtgctggct
     3301 agatagcgct cccgcagcga ccgacgggac ggcgggcggc cgtcggtacc gcgaccgacg
     3361 gggcgtcgac cctcagtacg tcacgaccac ctcgacgacc atgccctgga agcgcccgtt
     3421 gaacaggcca gggtcctccg aaccagccgg accccagccg ccggtgagca gcttctggta
     3481 ctcgccgtcc tgcagccgct cgtacgggca ggggttgtcg tcggtgggcg ggggcaccgt
     3541 ctggcccgtc cggtagccga ccgcaagcgt cgacccgtag tagtacgggt cgtagaagcc
     3601 ggtgccggtg gcctcgtagg tggtggcgcc cggcacgagc ttgatgccaa ggtagatgac
     3661 ctggcacacg cgggggttac ctgcgccgtc cgcgtaggac gcggcgtgat agaaccagcg
     3721 ccccgcgaag aggtcctgct tctccttgcc gatcactgtc gccgtggctt gagctaccag
     3781 ggccgcgccg ggcgccacct tgccggagcc gtcgtccttg tagacctcga cggctatggt
     3841 gtcgcgcagg tcgtccgtga tggcgttcgg ggcgttggcg acgtacagga cctgcgagcg
     3901 cgtggtctcg aggctctgcc cttcctgccc caagtagtcg taaaggtagc cgtactcgcc
     3961 gctcgtcgac cacctgtaca cgtagctccc accgtcgagc ggggcgtcta tcacgacctt
     4021 gaagtcggtg gacggcgcgt acttgcccac ctggccctct gacggcgtga gcttagccga
     4081 tacttccgtc ctgatcactt cccacgccgc accaggtggg ttgccggcca tgtcggagac
     4141 caccgccacg acgtcggcgc ctgtcagcac cagcgcgacg atcttgagga tcccgccggc
     4201 cgagccgacg gcgcgagcga aagcgcg
//
LOCUS       DAOQYE010000055         4150 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_21934_length_4150_cov_3.161661, whole genome shotgun sequence.
ACCESSION   DAOQYE010000055 DAOQYE010000000
VERSION     DAOQYE010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 4150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4150
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21934_length_4150_cov_3.161661"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="PKN52_01715"
     CDS             complement(<1..308)
                     /locus_tag="PKN52_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98685.1"
                     /translation="MPRLGADGAPPALPISTLLVGALAVALGGVWLLVSPAAFMGYLG
                     GPRVVALNHLFTLLYVGLVFAGTLQQLPAVMFVTKLVWPRLGWLTLPLLVAGSVLVVV
                     G"
     gene            complement(318..539)
                     /locus_tag="PKN52_01720"
     CDS             complement(318..539)
                     /locus_tag="PKN52_01720"
                     /inference="COORDINATES: protein motif:HMM:NF013379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HNQ98686.1"
                     /translation="MAEKFLDNRGLEPPNPMIRTLETLEGMARGDVLVIHNDRVPIYL
                     LPQLADAGASYEVEEQADGSAKVRITKGE"
     gene            576..941
                     /locus_tag="PKN52_01725"
     CDS             576..941
                     /locus_tag="PKN52_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98687.1"
                     /translation="MTYVMVAATAPCEATGVVGAEAPHTHSAGLTAEGRRTLLDTRVS
                     RILDAHPDALDTLIRHGFTPLAQAPLRFALAHTVNLGQAIRLRGLGEAEVAALLEALA
                     GMGLPGLLARPDAAAGEKV"
     gene            943..1269
                     /locus_tag="PKN52_01730"
     CDS             943..1269
                     /locus_tag="PKN52_01730"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNQ98688.1"
                     /translation="MALYKTGELARFSERGPVPRPLVVGDHMAVMLLCLQKHQEIKAP
                     ESDAAETVFMVVSGTGTVRENDEVHQVGPGDVVHLPPGTRKALQAGEDTFTVIGVRQL
                     GAKHVT"
     gene            1280..1477
                     /locus_tag="PKN52_01735"
     CDS             1280..1477
                     /locus_tag="PKN52_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HNQ98689.1"
                     /translation="MYVDMDACIECGICDQIAPGIREDVSHVPATSLTIDAMASCPTG
                     AIKWAEGGTDEAGRRQDHPAA"
     gene            1440..1664
                     /locus_tag="PKN52_01740"
     CDS             1440..1664
                     /locus_tag="PKN52_01740"
                     /inference="COORDINATES: protein motif:HMM:NF021520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HNQ98690.1"
                     /translation="MKQVDVRTIPPRERHPLLFRTFDDLETGQSFELVNDHDPKPLYY
                     QFQVERAGTIEWEYLEQGPQTWRVRIGKIG"
     gene            1677..2135
                     /locus_tag="PKN52_01745"
     CDS             1677..2135
                     /locus_tag="PKN52_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98691.1"
                     /translation="MSKWQVAFLRAALGWLVATGLLGVLVSIWPGLSGPFRTTHAHMG
                     VVGFFLAMVMGVAFWMLPRPGGIRQTNWEATTFLLLQAGLVMRVVSEPAWRTTLGRGW
                     HVLFVTSGVLVLAAMVVFALAMSRRVVTIDTLREKGSGRRKSAAPRDPTD"
     gene            complement(2149..2739)
                     /locus_tag="PKN52_01750"
     CDS             complement(2149..2739)
                     /locus_tag="PKN52_01750"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HNQ98692.1"
                     /translation="MNPNRVLEGAGMTDNGSASGNQEAPGGAPIPPVEPVGQGGHGAA
                     TPSNVATAAKPDVGKRAIAWIIDGVIAGILSSIPFLGSLLGAAYILTRDGFEFDFMDG
                     RSLGKKLMKLRPVRDDGGKMDLTTSIKRNWPLAIGMLGAVASYAPLLGMFGLTLLLSL
                     VGAIFGLVEIYFVLTSPDGRRYGDKFAGTHVVETQN"
     gene            2940..>4150
                     /locus_tag="PKN52_01755"
     CDS             2940..>4150
                     /locus_tag="PKN52_01755"
                     /inference="COORDINATES: protein motif:HMM:NF024891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNQ98693.1"
                     /translation="MVPTGPGVGLTSVCLGLVRVLEREGLRVGFIKPIRQPGDDSAPE
                     RSTEFVRRSTTIVPGEPMLFDSAEALLAAGRHSELLQALVGRYAAAAAGADVVIVEGL
                     AATAEHPNLDALNSDIARALDCQVLLVTTPLPSLHALDDRLELAAQPFGGAGSEHLLG
                     VVINQLNAPRMEVMGATRTNLRVPEVEQTAEGLLREMRIFRHPGFALVGAIPFSPAMV
                     SPRTLDVAKHLGATVLHEGDLASRRVIDVSLVARTVAHMTHRLRPDALLITPADRDDV
                     ILAVCMAALNGVPLAGLVLTGDMEPNADVMKLCEKAIATGLPVLAVTSDSYVTAARAA
                     AMNLEVPSDDVERMERVMKVAAEHLDVTTLKARVGGRRVLRLSPPAFLYRVVELARAA
                     GKRIVLPEGEEP"
BASE COUNT          646 a         1439 c         1426 g          639 t
ORIGIN      
        1 ccgacgacca ccaagacgct gccggcgacc aacagcggca gcgtgagcca ccccaatcgc
       61 ggccacacga gcttcgtcac gaacatgacc gcgggcagct gctgcaaggt gcccgcgaac
      121 acgagcccga cgtagaggag agtgaagagg tggttgaggg ccaccacgcg cggcccgccg
      181 aggtaaccca tgaaggccgc gggggagacg agcagccata ccccgcctag cgccactgcc
      241 aaggcgccga cgaggagggt ggagatcggc aacgcaggcg gggcgccgtc cgcgccgagg
      301 cgcggcatca ccgctgctca ctcccccttg gtgatacgga ccttggcgct gccgtcggct
      361 tgctcctcca cctcgtagct ggcgccggcg tcggcgagct gcggcaggag gtagatgggt
      421 acacggtcgt tgtggatgac gagcacgtcg ccccgtgcca tgccctccag ggtctccagc
      481 gtgcggatca tggggttggg tggctcgagg ccacggttgt cgaggaactt ctcggccatg
      541 ccatcaagct tccgcccgga gggcctcgcg ttccaatgac ctacgtcatg gtcgcggcga
      601 cggcgccctg cgaggctacg ggcgtggtag gggccgaagc accgcacacg cacagcgccg
      661 gcttgaccgc cgaagggcgt agaacgctcc tggatacgcg cgtctcgcgc atcctcgacg
      721 ctcaccccga cgcgctcgac acgctcatcc ggcacggttt cacgccgctt gcgcaagcac
      781 cgctgcgctt cgcgctcgcg cacaccgtga acctcgggca ggcaatccgc ctgcgcggcc
      841 tcggcgaggc cgaggtggcg gccctcctcg aggcgctcgc tggcatgggg ctgcccgggc
      901 tgctggcgcg gcccgacgcc gccgcaggcg agaaggtctg aggtggcgct ctacaagacg
      961 ggggagctcg cgcgcttctc ggagcggggg cccgtccccc gccccctggt cgtcggcgat
     1021 cacatggccg tcatgctcct atgcctccag aagcaccagg agatcaaggc gccagagagc
     1081 gatgcggccg agacggtctt catggtggtg agcggcaccg gcacggtgcg tgagaacgac
     1141 gaggtgcatc aggtcggacc gggcgacgtc gttcacttgc cgccgggcac ccgcaaggcc
     1201 ctgcaggccg gcgaggacac tttcaccgtg atcggcgtgc ggcaactggg ggcgaagcat
     1261 gtcacttaga cccgatgaga tgtacgtgga catggacgcg tgcatcgagt gcggcatctg
     1321 cgaccagatc gcgcccggca tccgcgagga tgtcagccac gtcccggcca cgagcctcac
     1381 catcgacgcc atggctagtt gcccaaccgg agccatcaag tgggccgaag gaggaaccga
     1441 tgaagcaggt cgacgtcagg accatcccgc cgcgtgagcg ccacccgctc ctcttcagga
     1501 cgttcgacga cctcgagact ggccagtcgt tcgagctcgt caacgaccac gatccgaagc
     1561 cgctctatta ccagttccag gtcgagcggg ctggaacgat cgaatgggag tacttggagc
     1621 agggaccgca gacgtggcgg gtgcgcatcg gaaagatcgg gtagcgcctc ggccgcgtga
     1681 gcaagtggca ggtcgctttc ctgcgggccg cgctcggctg gctcgtggcc accgggctgc
     1741 tcggcgtcct cgtgagcatc tggccgggcc tgtccgggcc gttcaggacg acgcacgcgc
     1801 acatgggcgt cgtcggcttc ttcctggcca tggtcatggg cgtggcgttc tggatgctgc
     1861 cccggcccgg cggtatcagg cagacgaact gggaggccac gaccttcctg ctcctgcagg
     1921 ccgggctcgt catgcgggtg gtgagcgagc cagcatggcg caccacgttg gggcgcggct
     1981 ggcacgtact tttcgtaacg agcggcgtgc tcgtgctggc ggccatggtc gtcttcgcgc
     2041 tcgccatgag tcgccgagtg gtcaccatcg acacgctcag ggagaaaggg tcggggcgcc
     2101 gcaagagcgc ggcgccccga gacccgaccg actaggtacg ggcctggatc agttctgcgt
     2161 ctccacgacg tgcgtgccgg cgaacttgtc gccgtagcgg cgtccgtcgg gcgaggtgag
     2221 cacgaagtag atctcgacca ggccgaagat ggcgccgacg agggacagca ggagcgtcag
     2281 accgaacatg ccgagcagcg gagcatagct cgccacggcg ccgagcatgc cgatggcgag
     2341 cggccagttc cgcttgatcg aggtcgtcag gtccatcttg ccgccgtcgt cgcgtacggg
     2401 ccgcagcttc atcagcttct tgccaagcga ccggccgtcc atgaagtcga actcgaaccc
     2461 gtcgcgcgtg aggatgtaag cggcgccgag caggctgccc aagaagggga tggaactcag
     2521 gatcccggct atcaccccgt cgatgatcca agcgatggcc cgcttgccga cgtccggctt
     2581 cgcggcagtc gccacgttgc tgggcgtggc cgcgccatga ccgccctgac cgacgggttc
     2641 gaccggtggg atgggcgcgc cgcctggcgc ttcctggtta ccgcttgcag agccgttatc
     2701 cgtcatgccc gctccttcca ggacgcggtt ggggttcatc gttcgaagcg tccggcacct
     2761 tagtgtaggt cacgcgtcgc gcgccggtat gtggcgatta gggcaccgca tcgggcgcgg
     2821 gcgcgggacc tatgacctgc agccgcgcag ggtcgggcag cgtaaggtgt tgcgggaggg
     2881 cggtgtgtct tcacgcgcgc cccgtgcgag gttcgatgtc gtcagcgcat gctttctacg
     2941 tggttcccac cggccccggc gtgggtctca cctccgtgtg tctcgggctc gtcagggtgc
     3001 tcgagcgcga ggggttgcgc gtcggcttca tcaagccgat ccgccaaccg ggcgacgact
     3061 ccgcgcctga acgctccacc gagttcgtgc gccgtagtac caccatcgtg cctggggagc
     3121 ccatgctctt cgactctgcc gaggcgttgc ttgctgccgg ccgccactcc gagctgctcc
     3181 aggcgctcgt cggccgctac gccgccgcgg ctgccggagc ggacgtcgtg atcgtcgagg
     3241 gcctcgccgc caccgccgag catccgaacc tcgacgcgct caactccgac atagcgcgcg
     3301 cgctcgactg ccaggttctg ctcgtcacga cccctctgcc gagcctccac gccctcgacg
     3361 accgcctcga gctggcagcc caaccgttcg gcggcgccgg gagcgaacat ctgctcggcg
     3421 tggtcatcaa ccagttgaac gcgccgcgca tggaggtcat gggcgccacg cgcaccaacc
     3481 tgcgcgttcc ggaggtcgag cagacggccg agggcctgct cagggagatg cgcatcttca
     3541 ggcaccccgg cttcgccctg gtgggcgcca tcccgttcag cccggccatg gtctctccgc
     3601 gcaccctcga cgttgccaaa cacctcggcg ccacggtcct gcacgagggc gacctcgcga
     3661 gcaggcgtgt gatcgacgtg tcgctcgtcg cgcgcaccgt cgcgcacatg acccaccggc
     3721 tgcggccgga cgcgctcctg ataactccag cggaccgcga cgacgtcatc ctcgccgtct
     3781 gcatggccgc cctgaacggc gtgccgctcg ccggcctcgt cctcaccggc gacatggagc
     3841 ccaacgccga cgtcatgaag ctctgcgaga aggccatcgc caccggcctg ccggtcttgg
     3901 cggtcacgag cgactcctac gtcacggcgg cgcgcgcagc cgcgatgaac ctcgaggtgc
     3961 ccagcgacga cgtggagcgc atggaacggg tcatgaaggt cgcggccgag cacctcgacg
     4021 tcaccaccct caaggcgcgc gtcggcggcc gccgcgtcct gcgcctctcg ccgccggcgt
     4081 tcctctaccg cgtggtcgag ctcgcccgcg cggccggcaa gcgcatcgtg ctgccggagg
     4141 gcgaggagcc
//
LOCUS       DAOQYE010000056         4131 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_22058_length_4131_cov_3.079490, whole genome shotgun sequence.
ACCESSION   DAOQYE010000056 DAOQYE010000000
VERSION     DAOQYE010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 4131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4131
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22058_length_4131_cov_3.079490"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..881
                     /locus_tag="PKN52_01760"
     CDS             <1..881
                     /locus_tag="PKN52_01760"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HNQ98694.1"
                     /translation="LGIDGMKTDGGEHLWGRSLRSHDGRRGLELFNTYVNDYVGAYHS
                     FIQRVTGGDGVTFSRSGYTGAQRFPIHWAGDEDSTWSAYRASVRAGLAAGVSGIGMWA
                     WDLAGFSGEIPPADLYMRSAAMAAFCPIMQFHSEPHGATERRDRTPWNIAERHGDPNA
                     LKVYRRYAHLRMRLRDYLHGEAEAAAAAGLPLMRYPALAYPDRHDFLAADEYAYLLGR
                     DLLVAPVTERGVETREVRLPPGAWVDLWSGSELAGDRVFHAPATLDTIPVFVRADSER
                     LAFLLSSASGLPGSGA"
     gene            885..1754
                     /locus_tag="PKN52_01765"
     CDS             885..1754
                     /locus_tag="PKN52_01765"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNQ98695.1"
                     /translation="MYERGWRRAGVLALFLGPSLIGLLAFTVGPMLTSLGLSFTNWDL
                     LTPAKFVGGANYQRLISDAEFWHTLKNTLTFLLGYVPLVMCIGLLVAVLLNSAIPLSH
                     AFRVMYFVPVVTSWVAVALVWKWLYNPVYGLVNNALSAIGVAGPAWLFDPNWAMPAVI
                     LTSVWKDTGFVMIIMLAGLQGIPREYYEAASIDGATRAQSLWSITIPLLAPTLVFCLS
                     ISLIGSFQVFDQVYVMTDGGPAGATMVLVERIVSHAFSYSRMGYASALSWVLFLIIFV
                     VTVVFYQLRRWRA"
     gene            1751..2650
                     /locus_tag="PKN52_01770"
     CDS             1751..2650
                     /locus_tag="PKN52_01770"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNQ98696.1"
                     /translation="MRQELPPVSAGTLTGGARIGRPNRRRFPLRTVVLTLVALAGGLL
                     MITPFLWAIGTSLKPSGALLSLPPQLVPSEPTVSAYTTVITRFPFLRVFGNSLLVAIA
                     STLGQLVVCSMSGYAFARFRFPGRDALFTVYLATLMVPFAVVITPLFIFVTRLGWANS
                     YAGLIIPGMFSAFGTFFMRQFFLSIPRELEEAATIDGASTLATFFRVVLPMTGPALAT
                     LTVLSFMGSWNSFLWPLLIINDRALMTLPIALSTLQGLYPGQTQWNVVMAGTVLATIP
                     TIIVFLFAQRWVIEGVAGSGVKG"
     gene            2647..3705
                     /locus_tag="PKN52_01775"
     CDS             2647..3705
                     /locus_tag="PKN52_01775"
                     /inference="COORDINATES: protein motif:HMM:NF012926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HNQ98697.1"
                     /translation="MNADQDVSTILAAQDAGGAYPAGPGFSQYQQCWLRDGAFIAHAL
                     DLVGEHGSVARFHAWAARCLAPLGPYVQELRGRREAGEPITEFEFLPARFTLDGRWLK
                     DGWPNFQLDGYGQWLWSLAGHLTATGQTVVPAALAPAVTVVVDYLEAFWNEPCYDSWE
                     EHRSQLHTATLASIAAGLRDIVPLVGPELGARAARVHAAARAFIEAECVEGGAFVKYV
                     RNPAVDASLLWLATPFDLVSPTDERMERTVARIESELLGGGLRRYRADTYYGGGEWVL
                     LTAWLAWHKRRLGDDAGAARLLAWVDEQRGSDGALPEQVPGADCHPYFLDYWTTNWGP
                     SAGPLLWSHAMVVAARVG"
     gene            3910..>4131
                     /locus_tag="PKN52_01780"
     CDS             3910..>4131
                     /locus_tag="PKN52_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98698.1"
                     /translation="MTRAGAAPVVLSLDVGGSSVKSGLVTADGALPAGPFLVALDSRG
                     PATGIVATLAEAITRLLERAAGREVLGVGV"
BASE COUNT          558 a         1438 c         1400 g          735 t
ORIGIN      
        1 agctcggcat agacggcatg aagacggacg gcggcgagca cctgtggggg aggagtctga
       61 ggtcccacga cggccggcgc ggcctcgagc tcttcaacac ttacgtcaac gactacgtgg
      121 gggcctacca ctcgttcatc cagcgggtga cgggcggcga cggcgtcacg ttctcccgct
      181 cgggctacac gggcgcccag cgcttcccca tccactgggc gggcgacgag gacagcacgt
      241 ggagcgccta ccgcgcctcg gtacgtgccg gcctcgcggc cggcgtgagc ggcatcggca
      301 tgtgggcgtg ggacctggcc gggttcagcg gtgagatccc gcccgccgac ctctacatgc
      361 gctccgccgc catggcggcc ttctgcccca tcatgcagtt ccactccgag ccgcacgggg
      421 ccaccgagcg gcgcgacagg actccgtgga acatcgccga gcgccacggc gacccgaacg
      481 cgttgaaggt ctaccgtcgc tacgcgcacc tgcgcatgcg cctgcgcgac tacctacacg
      541 gcgaagcgga ggcggccgca gccgccggac tgccgctcat gcgctacccg gcgctcgcct
      601 accccgatag gcacgacttc ctggcggccg acgagtacgc ctacctgctt ggcagggacc
      661 tgctcgtggc tcccgtcacg gaacgcggcg tggagacgcg cgaggtcagg ctgccgcccg
      721 gtgcttgggt cgacctatgg tcgggcagcg aactggccgg tgaccgtgtg ttccacgccc
      781 ctgcgacgct tgacaccatc cccgtcttcg tgcgcgccga cagcgagcgc ttggcgttcc
      841 tgctgagctc agcctccggc ttgccgggaa gcggcgccta agccatgtac gagcgcggtt
      901 ggcggcgcgc cggggtgctg gcgctcttcc tcggaccgag cctcatcggc ctactggcgt
      961 tcacggtagg gcccatgctg acgtcgctcg ggctgtcttt cacgaactgg gacctgctga
     1021 ccccggcgaa gttcgtgggc ggcgccaact accagcggct catctcggac gccgagttct
     1081 ggcacacgct caagaacacc ttgacgttcc tcctcggtta cgtgccgcta gtgatgtgca
     1141 tcgggctgct cgtcgccgtg ctcctcaaca gcgccatccc gttaagccac gcgttccgcg
     1201 tcatgtactt cgttccagtc gtcacgtcgt gggtggccgt cgcgctcgtg tggaagtggc
     1261 tttacaaccc cgtgtacggg ctggtgaaca acgcgctctc ggccatcggc gtcgcgggtc
     1321 cggcgtggct cttcgatccg aactgggcca tgccggccgt gatcctgacg agcgtgtgga
     1381 aggacacggg cttcgtcatg atcatcatgc tcgccggtct gcagggcatc ccgcgcgagt
     1441 actacgaggc ggcaagcatc gacggcgcga cgcgcgccca atcgctatgg tcgatcacca
     1501 tcccgctcct cgccccgacc ctcgtgttct gcctcagcat cagcctcata ggctcgttcc
     1561 aagtgttcga ccaggtgtac gtgatgacgg acggcggacc ggcgggcgcc accatggtcc
     1621 tcgtcgagcg gatcgtctcg cacgcgttct cctacagccg gatgggctac gcttccgcgc
     1681 tctcgtgggt cttgttcctg atcatcttcg tggtgaccgt ggtcttctac cagctgaggc
     1741 ggtggagggc gtgaggcagg agctgccgcc ggtgtcggcc ggcaccctca cgggcggtgc
     1801 gcgcataggt cgacctaacc ggcgccgctt cccgctgcgc acggtcgtcc tcaccctcgt
     1861 ggcgctcgcc ggcgggctcc tcatgatcac gccgttcctg tgggcgatag gcacgtcgct
     1921 gaagcccagc ggggcgctcc tcagcctgcc gccgcagctc gtcccgtccg agccgaccgt
     1981 ctcggcctac acgaccgtca tcacgcgctt cccgttcttg cgcgtcttcg gcaactccct
     2041 gctggtggcc atcgcctcga cgctcggcca gctcgtcgtg tgttcgatgt cgggctacgc
     2101 cttcgcgcgc ttccgcttcc cgggccgcga cgcgctcttc accgtctacc tcgccaccct
     2161 catggtgccg ttcgccgtgg tgatcacgcc gctgttcatc ttcgtgacgc gcctcgggtg
     2221 ggcgaacagc tacgccggcc tcatcatccc gggcatgttc agcgccttcg gcacgttctt
     2281 catgcgccag ttcttcctct ccatcccgag ggagctggag gaggcggcca ccatcgacgg
     2341 cgcctccacc ctggctacct tcttccgcgt agtgctcccg atgaccgggc cggcgctggc
     2401 gacgctgacg gtcctctcgt tcatggggtc gtggaacagc ttcctttggc cgctcctcat
     2461 catcaacgac cgcgcgctca tgacgctccc catcgccctc tccaccctac aggggctcta
     2521 cccggggcag acgcagtgga acgtcgtcat ggccggtacc gtgctggcca ccatcccgac
     2581 gatcatcgtc ttcctcttcg cccaacggtg ggtcatcgag ggcgtggccg ggtccggggt
     2641 gaaggggtga acgccgacca ggacgtgtcg acgatcctcg ccgcccagga cgcggggggc
     2701 gcctacccgg ccggcccggg cttctcgcag taccagcagt gctggttgcg cgacggcgcg
     2761 ttcatcgccc acgcccttga cctcgtcggt gaacatggca gcgtcgcgcg tttccacgcc
     2821 tgggccgcgc gctgccttgc gccgctcggg ccgtacgtcc aggagctgcg ggggcggcgc
     2881 gaggcaggtg agcccatcac cgagttcgag ttcctgccgg cgcgcttcac gctggacggt
     2941 cggtggctga aggacggctg gccgaacttc cagctcgacg gttacggaca gtggctgtgg
     3001 agtctggccg gtcacctgac ggccaccggc cagactgtcg tgccggccgc cctggctccg
     3061 gccgtgacgg tagtggtgga ttacctggag gcgttctgga acgagccgtg ctacgactcg
     3121 tgggaagagc acaggagcca gctccatacg gcgacgttgg cgtcgatcgc cgccgggttg
     3181 cgtgacatcg tcccgttggt cggtcccgag ctcggggcgc gggcggcccg cgttcatgcc
     3241 gcggcccgcg ccttcatcga agcggagtgc gtggagggcg gcgccttcgt caagtacgtc
     3301 aggaacccgg ccgtcgacgc gtcgttgcta tggctcgcca cgcccttcga cctcgtgagt
     3361 ccgacggacg aacgcatgga gcggaccgtc gcgaggatcg agagcgagct cctgggcggc
     3421 ggtctgaggc ggtacagggc cgatacctat tacggcggcg gcgagtgggt gctcctgacg
     3481 gcgtggctgg cgtggcacaa gcgccggttg ggggacgatg ccggcgccgc acgcctcctc
     3541 gcgtgggtgg acgagcagcg ggggagcgac ggcgcgctgc ccgaacaggt gcccggtgcc
     3601 gactgccatc cgtacttcct cgactactgg acgacgaact ggggtccttc ggccggaccg
     3661 ttgctgtggt cgcatgcgat ggtggtcgcg gctcgggtag ggtgatgacc gagtctcgct
     3721 gaaccggtcg cggcaccggg cctccggtgg cgttctcgat ggggaactca agacttcgac
     3781 cgtgacgcgc ccgctggcgc ggctcgaccc gtatcagata ttcggctcaa ggactgacca
     3841 gtaggacgag aggggctgcg cgatgtggct agcctacgag ctcgagacgc tcccgcccgg
     3901 cacatcggca tgacccgggc cggcgcggcg ccggtcgtgt tgagcctcga cgtgggcggc
     3961 agctccgtga agagcggtct cgtcacggca gacggcgctc tgcccgctgg acctttcctg
     4021 gtcgccctcg acagccgcgg tccggctacc ggcatagtcg ctacgctggc cgaggccatc
     4081 acgcggctac tggagcgcgc ggctgggcgc gaggtgctcg gcgtcggcgt c
//
LOCUS       DAOQYE010000057         4086 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_22397_length_4086_cov_3.676755, whole genome shotgun sequence.
ACCESSION   DAOQYE010000057 DAOQYE010000000
VERSION     DAOQYE010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 4086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4086
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22397_length_4086_cov_3.676755"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1028
                     /locus_tag="PKN52_01785"
     CDS             <1..1028
                     /locus_tag="PKN52_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98699.1"
                     /translation="LGRIADLQTKVARLTTVTVKLKQARASARTFETRTKALAAEVGR
                     PFDGLGPSVAVGEWLQAAASAEALEADLAEKRGRMTNLIGAGQQKLETLFGSRAAEAA
                     TALDERDPQAWEQGLTQWRARAEDLERQADGLTRRTGALKQTLDDLGRSADLPGYLAA
                     LESSGNSARLEARRWLVARLAKDLISSTRAAYEREKVPEVLRRTGHHLAEVTGGRYVR
                     VHLGDDGALRAFTPADEPSEVGTLSRGTQEQLYLAIRFGLVESFSSGHAAMPLVLDEV
                     MVNADPERAERLARSLVELARRHQLLYLTCHPETARLLVGSAPSGAVHIRLAGTRTVQ
                     TVRPPGG"
     gene            1099..1842
                     /locus_tag="PKN52_01790"
     CDS             1099..1842
                     /locus_tag="PKN52_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98700.1"
                     /translation="MQHIDLLWTGGWDSTFRLLEAVLQQRLTVQPHYVIDDPPRPSTD
                     AELAAMARIRAGALLLDPAAEDRILPTVVQHRTAVPRDEQIRQAYKAVMQQVYIGGQY
                     VWLAEYALSKGIRLELAIHVDDRAHLAIKDHVESVDDGFGSTYAVRPGDDSPNAILFR
                     QFRFPILRLSKLDMQARATEHGYAHLLEETWFCHTPRAGKPCGLCAPCQFAIQEGLGR
                     RVPANRRLLYRVLAPYLRAKTLAARWRAR"
     gene            2067..2675
                     /locus_tag="PKN52_01795"
     CDS             2067..2675
                     /locus_tag="PKN52_01795"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNQ98701.1"
                     /translation="MSALPRPTTARGEATRRKLLAAAEAEFGENGFAATAVSDITRRA
                     GVAQGTFYLYFPTKADALRQLVQDMGRQLRHTLTAGTAGAKDRLEVERRGLEAFIDFA
                     LAHQNLYKVVMESQFVDDSIYREYYQTLADSYAVNLRRAQERGELRDLDAGAQAWALM
                     GAAHFLGLRYAIWERRRPPQHVLDATFEVLARGIAPMRDAAG"
     gene            2672..3694
                     /locus_tag="PKN52_01800"
     CDS             2672..3694
                     /locus_tag="PKN52_01800"
                     /inference="COORDINATES: protein motif:HMM:NF005308.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase"
                     /protein_id="HNQ98702.1"
                     /translation="MTMAGLTGLGTYVPAGRLSAAELAAASGLPEWVVKDKLGIAQRV
                     LPGQDDHPTAMGVWAAERALADAGVRPEDVDVVISITEEHKEYPVWTSGIKLAYDVGA
                     RNAYAYDVGQKCGTAVLALKQARDLIRADESVNTVLVAGGYRNSDLVNLQDPDVRFMY
                     NLGAGAGAAVVQRSVGHELLGAHILTDGSFSLDILVPVGGTKAPLTHANLSDYRLRVT
                     DPAGMKERLEPVSLENFVRVVEGAVARSGATTADIAYLAMLHVKRSAHDYLMRRLGLR
                     DEQSIYLSDYGHLGQVDQLLSLELARDQGRLRAGDLAVAVAAGVGYVWNAIAVRWGDG
                     VTRAAA"
     gene            3708..>4086
                     /locus_tag="PKN52_01805"
     CDS             3708..>4086
                     /locus_tag="PKN52_01805"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNQ98703.1"
                     /translation="MILDVAARRAELTPGRDALWWDGRWYTYLELNERATRTAALLAG
                     MGVTKGDRVMIAAHNHVAHFDLVLATAKLGFAYTPLNVRLSTAELHGIGEYLRPSVVL
                     HDAEHAALAAAAGEGATLLDLTTF"
BASE COUNT          674 a         1440 c         1402 g          570 t
ORIGIN      
        1 tcctcggccg gatcgccgac ctgcaaacca aggtcgctag gctcacgacc gtaacggtga
       61 agctcaagca agcccgcgcc tccgcccgga cgttcgaaac gcggaccaag gctctggccg
      121 ccgaggtggg gcgcccgttc gacggcctcg gcccgtccgt ggcagtgggc gagtggctcc
      181 aggccgcggc aagcgccgag gccttggagg ccgacctggc ggagaagcgc gggcgtatga
      241 cgaacctgat cggagccggt cagcagaagc tggagaccct cttcggatcg agggcagcgg
      301 aggctgcgac cgcgttggac gaacgcgacc cgcaagcatg ggagcagggg ctcacccagt
      361 ggcgagcccg ggcagaagac ctcgaacgac aagccgatgg actcacccga cgcacggggg
      421 cgctgaagca gaccctagac gacctcgggc gctcggccga cctccccggc tacctggccg
      481 cgctcgagtc ctcgggaaac agcgcgcgcc tcgaggcgcg ccggtggctc gtcgcgcgtc
      541 tcgccaagga cctgatctcc tcgaccaggg ccgcctacga acgcgagaag gtgccggagg
      601 tactccgcag gacgggccac cacctagccg aggtgacggg cggtcgctac gtgcgcgtcc
      661 atctcggcga cgacggcgcg ctacgcgcgt tcacgccggc ggatgagccg tccgaggtcg
      721 gaacgctgag caggggcacg caagaacagc tgtacctcgc catccgcttc ggtctcgtcg
      781 agtctttctc gagtgggcac gcggccatgc cgctcgtgct cgacgaggtc atggtcaacg
      841 ccgaccccga gcgcgccgaa cgcctggctc gctcgctcgt agagctggct cggcggcacc
      901 agcttctcta tctcacgtgc catccggaga ccgcgcggct cctggtcggc agcgcaccga
      961 gcggcgcggt gcatatcagg ctggctggaa cgcgcaccgt ccagaccgtc cggccgcctg
     1021 gcggatgagc tatagcgcga ccgtacgagg cgtcgggatg acccgttggc tccgtcctgg
     1081 gccccataga atgcggtgat gcagcacatc gacctgcttt ggaccggcgg atgggactcg
     1141 accttccgcc tactagaggc cgttctgcag cagcggctga cggtccagcc tcactacgtc
     1201 atcgacgacc cgcccaggcc gtccaccgac gccgagttgg cggccatggc gcggatacgc
     1261 gctggagcgc tcctactcga tcccgcggcg gaggaccgga tcctgcctac cgtcgtccag
     1321 cacaggacgg ccgtaccgcg tgacgagcag atccgacagg cctacaaggc ggtcatgcag
     1381 caggtgtaca tcggcgggca gtacgtgtgg ttagcggagt acgcgctctc caagggcatc
     1441 cgactcgaac tggcgatcca cgtcgacgac cgcgcccacc tggcgatcaa ggaccacgtc
     1501 gagtcggtgg acgatgggtt cggaagcacc tacgccgtcc gccccggcga cgactctccg
     1561 aacgcgatcc tcttccggca gttccggttc ccgatcctgc ggttgagcaa gctcgacatg
     1621 caggcaaggg caaccgagca cggctacgct cacctgctcg aggagacgtg gttctgccac
     1681 acgccccgcg ccggcaagcc gtgcgggctc tgcgccccct gccagttcgc catccaggag
     1741 ggcctgggga ggcgcgtccc cgccaaccgg cgccttctat accgcgtgct cgcgccgtac
     1801 ttgcgtgcca agaccttggc ggcacggtgg cgcgctagat aggcaaccga ggcttgcccg
     1861 gctcgctcct gcgacgcggt ggcatcgcgt ggcgcgcggg gttccacggc gggcaggcct
     1921 aggccgatca cccactcact cgcacggacg cttgacagcc gagtccgctc gcgctctaat
     1981 tgctgacaac tgactcagta gtcaggttca tggctcctca cccggcgaca cggccgctga
     2041 acgccacgaa ccacggaagg gaagagttga gcgcacttcc acgcccaacc accgcgcgcg
     2101 gcgaagccac gcgccgcaag ctgctcgccg cggccgaggc cgagttcggc gagaacggct
     2161 tcgcggctac ggccgtatcg gacataacga gacgggccgg ggtagcccag gggacgttct
     2221 acctctactt cccgaccaag gcggacgcgc tgcgccaact cgttcaggac atggggcggc
     2281 agctccggca caccctcacc gcggggaccg cgggggccaa ggaccgcttg gaggtcgaga
     2341 ggcgcgggct ggaggcgttc atcgacttcg ccctcgccca ccagaacctc tacaaggtcg
     2401 tcatggagtc gcagttcgtc gacgactcga tctaccgcga gtactaccag acgctcgccg
     2461 attcctacgc cgtcaacctg cgacgcgctc aggagcgggg cgagctgcgc gacctcgatg
     2521 ccggcgccca ggcgtgggcg ctgatgggcg cggcccactt cctcggcctg cgctacgcca
     2581 tctgggagcg gcggcggcca ccgcagcacg ttctcgacgc cacgttcgag gtgctcgccc
     2641 gcggcatcgc gcctatgagg gacgcagcgg gatgaccatg gccggcctga ccgggctcgg
     2701 aacctacgtt cccgccggtc gcctctccgc cgcggagttg gccgccgcca gcgggctgcc
     2761 ggagtgggtc gtgaaagaca agctcggcat cgcgcagcgc gtcctgcccg gccaagacga
     2821 ccacccgacg gcgatggggg tgtgggcggc cgagcgcgca ctggccgacg cgggcgtgcg
     2881 acccgaggac gtcgacgtcg tcatctccat cacggaggag cacaaggagt acccggtctg
     2941 gacgtccggc atcaagctcg cgtacgacgt cggcgcgcgc aacgcctacg cctacgacgt
     3001 ggggcagaag tgcggcaccg ccgttctggc gctcaagcag gcccgcgacc tcatcagggc
     3061 cgacgagagc gtgaacaccg tgctggtggc cggcggctac cgcaactccg acctcgtcaa
     3121 cctccaggac ccggacgtgc gcttcatgta caacctgggc gccggcgcag gcgcggccgt
     3181 ggtgcagcgg agcgttgggc acgaactgct cggcgctcac atcctgacgg acggcagctt
     3241 ctcgctggac atcctcgtgc ccgtcggcgg cacgaaggcg ccgctgacgc acgcgaacct
     3301 ctcagactat cgcctgcgcg tcaccgaccc ggccggcatg aaggagcgcc tcgagccggt
     3361 cagcctcgag aacttcgtca gggtcgtcga gggggcggta gcgcgcagtg gcgcgaccac
     3421 agccgacatc gcctacctgg ccatgctgca cgtgaagcgc agcgcccacg actacctcat
     3481 gcggcgcctg gggttgcgcg acgagcagag catctacttg agcgactacg gtcacctcgg
     3541 acaggtggac cagctcctct cgctcgaact cgcccgcgac cagggcaggc tgcgggcggg
     3601 cgacctcgcc gtggccgtgg ccgccggtgt cggctacgtg tggaacgcca tcgccgtgcg
     3661 gtggggcgac ggcgtgacaa gggcggcggc atgacaggag cggcggcatg atcctcgacg
     3721 tggccgcccg ccgggcggag ttgacgcccg ggcgcgacgc cctctggtgg gacggtcgct
     3781 ggtacacgta tctggagctg aacgaacgcg ccacgcgcac ggcggcgctg ctggcaggca
     3841 tgggggtgac caagggcgac cgcgtcatga tcgcggccca caaccacgtc gcgcacttcg
     3901 acctggtgct ggcgacggcg aagctcggct tcgcctacac gcccctcaac gtgcgcctct
     3961 ccacggcgga actgcacggc atcggtgaat acctgcgtcc gagcgtcgtg ctgcacgacg
     4021 cggagcacgc ggcgctggcc gcagcggccg gtgagggggc gaccctcctc gacctgacga
     4081 ccttcg
//
LOCUS       DAOQYE010000058         4082 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_22428_length_4082_cov_2.686864, whole genome shotgun sequence.
ACCESSION   DAOQYE010000058 DAOQYE010000000
VERSION     DAOQYE010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 4082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4082
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22428_length_4082_cov_2.686864"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            57..1385
                     /locus_tag="PKN52_01810"
     CDS             57..1385
                     /locus_tag="PKN52_01810"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HNQ98704.1"
                     /translation="MIDRHAFPDDFTWGVATSSYQIEGSTSTDGCGPSIWGSFCAQPG
                     KIVDGSSGAVACDHYRRWQSDLDLIRELGVTAYRFSLSWPRILPEGRGRVEQRGLDFY
                     ERLVDGLLERGIEPWATLYHWDLPQALQDAGGWPERAVAHAFADYADLVTARLGDRVR
                     GWATLNEPWCSAFLGYESGEHAPGLRSRRKHLQASHNLLLAHGLALPRMRRNAPGSRH
                     GIVLNLNPAYPASPAPEDVLAAERFDGFFNRWYLDPVLRGSYPVDAWAGYGQDVPSVE
                     PGDLETISAPIDFLGVNFYSRAVVTEEPGTPWPSTRGVAVEGPHTAMGWEIRPKALTE
                     LLERLARDYALPDVHITENGAAFDDEERAGVYHDPERVAYLQAHVAALADAARAGVPV
                     AGYFAWSLLDNFEWAQGYTRRFGLVHVDFATQRRSLKESARWYRRFLSGG"
     gene            1445..1945
                     /locus_tag="PKN52_01815"
     CDS             1445..1945
                     /locus_tag="PKN52_01815"
                     /inference="COORDINATES: protein motif:HMM:NF015232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HNQ98705.1"
                     /translation="MLEPSLDLYGTTFDTVDGILRELLTKSRARYALIIDHKGFVLMH
                     ARALWAPKPPSLDSFATLVASNYAANRAIANLFGEEGFRETVQQGAEVGTYLEELGSE
                     ALLATVFDSTAQLGRVKLAAKHAADAIRVALEGATEAPPSMRFDAEFQQGATELLDGL
                     FGARNS"
     gene            1942..2532
                     /locus_tag="PKN52_01820"
     CDS             1942..2532
                     /locus_tag="PKN52_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylation factor-like protein"
                     /protein_id="HNQ98706.1"
                     /translation="MSTINFSAREINFKIVYYGPGLSGKTTNLKQIYQQVPAEAKGEM
                     VSLATEDERTLFFDFLPLDLGKVNGFKTRFHLYTVPGQVFYNSSRKLILRGVDGVVFV
                     ADSSMARLRANAESLRNLRENLQEYNLRLADVPYVIQANKRDLPDAIGMDALKAVLDR
                     EGKVQFYEAVAAKFEGVFEPLRGVATMVLEKLAQKA"
     gene            2597..3658
                     /gene="gcvT"
                     /locus_tag="PKN52_01825"
     CDS             2597..3658
                     /gene="gcvT"
                     /locus_tag="PKN52_01825"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HNQ98707.1"
                     /translation="MKRTPLFEAHEELGARMVEFAGYAMPLHYQAGINQEHLSVRQGV
                     GLFDVSHMGEVRVVGPGATRFLQYATLNDPARLKPGRGQYSMLPNATGGLIDDLFVYR
                     EGEDDYLVVANASNTAAVLLHLQDLAAGHECHVIDESDTVALLALQGPGAPLLLGQLV
                     DDDLGQLKRNATLDVVLSGIPVRLSRTGYTGEDGFEIFLRPADAPSVWDMLLAAGASP
                     CGLGARDTLRLEAGFPLYGHELTEASNPLCTPFAWVVKDKEFFGRDAMWDPACDERLV
                     GLRLTERGIPRQGYRVLDASGAPAGKVTSGALSPLTRDGIGFAWVRRDLAELGTELAV
                     EIRGQAVAAKVVQPPFHLT"
     gene            3709..>4082
                     /gene="gcvH"
                     /locus_tag="PKN52_01830"
     CDS             3709..>4082
                     /gene="gcvH"
                     /locus_tag="PKN52_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HNQ98708.1"
                     /translation="MKTPPELRYAESHEWVALQGSSATVGITDFAQDQLGDVVFVELP
                     AVGRRVAKGEQMAVIESVKTASDIYAPVSGVVAAVNDALDAKPELVNSEPYGEGWLVR
                     IEVADTSEVDGLLDATAYAASAS"
BASE COUNT          647 a         1483 c         1336 g          616 t
ORIGIN      
        1 cgtccatcgt cctggtacgt aacgcgcctt cggctccacc caacgaggag tgatgcatga
       61 tcgaccgcca cgccttcccc gacgacttca cctggggggt cgccacctcg tcctaccaga
      121 tcgagggctc gactagcacc gacggctgcg ggccgtccat ctggggctcg ttctgcgccc
      181 agccgggcaa gatcgtcgac ggctcaagcg gcgcggtggc ctgcgaccac taccggcgct
      241 ggcagtcgga cctcgacctg atcagggagt tgggcgtgac ggcctaccgt ttctccctca
      301 gctggccccg catcctcccg gaaggtcgcg gacgggtgga gcagcgcggg ctcgacttct
      361 acgagcgcct ggtcgacggc ttgctcgagc gcggcatcga gccgtgggcc acgctctacc
      421 actgggacct gccccaggcg ttgcaggacg ccggcggctg gccggagcgc gccgtcgccc
      481 acgccttcgc cgactacgcc gacctcgtta cggcccgcct cggcgaccgc gtgcgcggtt
      541 gggccacgct caacgagcca tggtgcagcg ccttcctcgg ctacgagagc ggcgagcacg
      601 ccccgggcct gcgtagccgg cgcaagcacc tccaggcgtc gcacaacctg ctcctcgccc
      661 acgggctcgc cctgccccgc atgcggcgca acgcccccgg atcgcgccac gggatcgtcc
      721 tgaacctcaa ccccgcctac ccggcgagtc cggctcccga ggacgtcctc gccgccgagc
      781 gcttcgacgg cttcttcaac cgctggtacc tcgaccccgt gttgcgtggc tcgtacccgg
      841 tcgacgcgtg ggcaggctac ggccaggacg ttccgagcgt cgaaccgggc gacctggaaa
      901 cgatctcggc ccccatcgac ttcctgggcg tcaacttcta cagccgcgcg gtcgtcacgg
      961 aggagccggg tacgccgtgg ccgagcaccc ggggcgtggc ggtcgaaggc ccccacaccg
     1021 ccatgggctg ggagatccgg ccgaaggcgc tgaccgagct gctagagcgc ctcgcccgcg
     1081 actacgcgtt gccggacgtc catatcacgg agaacggcgc cgccttcgac gacgaggagc
     1141 gcgctggcgt ctaccacgac cccgagcgcg tcgcctacct gcaggcgcac gtcgcggcgc
     1201 tggccgatgc cgcgcgcgcg ggggtgcccg tggcgggtta cttcgcgtgg agcctcctcg
     1261 acaacttcga gtgggcgcag ggctacaccc gacgcttcgg actggtccac gtcgacttcg
     1321 ccacccagcg gcgcagcctc aaggagagcg cccggtggta ccggcgcttc ctgtcggggg
     1381 gttgagcggg cgaagtgcat tgttctaccc cggctcggag cccccggctg ttacagtgac
     1441 gttcatgctc gagccgtccc tcgacctcta cggcaccacg ttcgacacgg tcgacggtat
     1501 cttgcgcgaa ctcctgacga agagccgggc ccgctacgcc ctgatcatcg accacaaagg
     1561 cttcgtgctc atgcacgcgc gcgccctgtg ggcgcccaag ccgccgtcgc tcgactcctt
     1621 cgccaccctc gtggcctcca actacgccgc caaccgcgcc atcgccaacc tcttcggcga
     1681 ggaggggttc cgcgagacgg tccagcaggg cgccgaggtc ggcacctacc tcgaggagct
     1741 cggcagcgag gccctcctgg ccaccgtgtt cgactcgacc gcccagctcg gacgcgtgaa
     1801 gctcgcggcc aagcatgccg ccgacgccat cagggtggcg ctcgaaggcg ccacggaggc
     1861 gccgccgagc atgcgcttcg acgcggagtt ccagcaaggc gcgacggaac tcctcgacgg
     1921 cctcttcggg gcgaggaact catgagcacc atcaacttct ccgctcgcga gatcaacttc
     1981 aagatcgtct actacggccc gggcctctcc ggcaagacca cgaacctcaa gcagatctac
     2041 caacaggtgc cggccgaggc caagggcgag atggtgtcgc tggccacgga ggacgagcgc
     2101 acgctcttct tcgacttcct cccactggac ctcggcaagg tcaacgggtt caagacgcgc
     2161 ttccacctct acacggtgcc tggccaggtc ttctacaact cctcgcgcaa gctgatcctg
     2221 cgcggcgtgg acggggtcgt gttcgtcgcg gactcctcca tggcgaggct gcgcgccaac
     2281 gcggagagcc tgcgcaacct gcgcgagaac ctccaggagt acaacctgcg tctcgccgac
     2341 gtgccgtacg tcatccaggc gaacaagcgc gaccttcccg acgccatcgg catggatgcc
     2401 ctcaaggccg tgctcgatcg cgagggcaag gtgcagttct acgaggccgt agcggccaag
     2461 ttcgagggag tgttcgagcc cctaaggggc gtcgcgacca tggtcctcga gaagctcgcc
     2521 cagaaagcct gagagttagc cggaagcgcg cctggacggc cgaaacgccg cccgggagcg
     2581 tgctatgttc gacccgatga agcgcacccc actgttcgaa gcacacgagg agctcggcgc
     2641 ccgcatggtc gagttcgcgg gttacgcgat gccccttcac taccaggccg gcatcaacca
     2701 ggagcacctg agcgtgcggc agggcgtcgg actgttcgac gtctcgcaca tgggcgaggt
     2761 gcgcgtggtg ggtccgggag ccacgcgctt cctgcagtac gcgaccctga acgacccggc
     2821 gcgcctgaag cccggccgcg gtcagtacag catgctcccc aacgcaacgg gcgggctcat
     2881 cgacgacctc ttcgtctacc gcgagggcga ggacgactac ctcgtagtcg ccaacgcgtc
     2941 caacacggcg gccgtgctgc tccacctgca ggacctggcc gccgggcacg agtgccacgt
     3001 gatcgacgag tccgacacgg tcgcgctgct cgcgttgcag ggtcctggcg ccccgctgct
     3061 ccttggtcaa ctcgtcgacg acgacctcgg tcagctcaag cgcaacgcga ccctcgacgt
     3121 cgtcctctcg ggtatccccg tgcgcctcag ccgcaccggc tatacgggcg aggacggctt
     3181 cgagatcttc ctgaggccgg ccgacgcccc ctccgtgtgg gacatgctcc tggcggccgg
     3241 cgccagcccc tgcgggctcg gtgcccgcga cacgctgcgc ctcgaggcgg gcttcccgtt
     3301 gtacggacac gagctgacag aggcctccaa cccgctctgc acgccgttcg cctgggtggt
     3361 caaggacaag gagttcttcg gccgcgacgc catgtgggac ccggcatgcg acgagcggct
     3421 cgtgggcctg aggctgacgg agcgcggcat cccgcgccag ggttaccgcg tgctcgacgc
     3481 ctccggcgcc ccggccggca aggtcacgtc cggcgcactc tcccctctca cccgtgacgg
     3541 catcggcttc gcctgggtgc gccgcgacct ggccgaactg ggcacggagc tcgccgtcga
     3601 gatcaggggc caggcggtcg ccgcgaaggt ggtgcagccg ccgttccacc tgacatgatg
     3661 cttagcggta cgagcgcggc agcacgccgc ttcacctagg agtcggacat gaagacaccg
     3721 ccagaactgc gttacgccga atcgcacgag tgggtcgccc tgcagggcag ctcggccaca
     3781 gtcggcatca cggacttcgc tcaggaccag ctgggggacg tggtgttcgt cgaactcccg
     3841 gccgtaggcc gccgcgtcgc caagggcgag cagatggccg tcatcgaatc ggtcaagacg
     3901 gcctcagata tctacgcccc cgtgtccggc gtcgtcgcgg ccgtcaacga cgcgcttgac
     3961 gcgaagccgg aactggtcaa ctccgagccg tacggcgagg gttggctcgt gcgcatagag
     4021 gtagccgaca cgagcgaggt cgacgggctg ctcgacgcga ccgcctacgc ggcgagcgcg
     4081 tc
//
LOCUS       DAOQYE010000059         4076 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_22474_length_4076_cov_3.282019, whole genome shotgun sequence.
ACCESSION   DAOQYE010000059 DAOQYE010000000
VERSION     DAOQYE010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 4076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4076
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22474_length_4076_cov_3.282019"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1031
                     /locus_tag="PKN52_01835"
     CDS             <1..1031
                     /locus_tag="PKN52_01835"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNQ98709.1"
                     /translation="LATRACPRRAARRAAALARVLVFSALSLLSGAFAQGEVRVYSVI
                     NEDDVKLLAELFTAETGIQVTYLRAATGELVSRVIAEKGAPQADVLLGGPSSQHIAIA
                     DTGALAQYSPAAAEALPAYAVSPEGYWTGFYLTALGIGVNEERFHRLYPDKPFPATWD
                     DLLDPAFKGEIVMTDPVASSTAYLFVQTQLQRLGWDAGWAYLEALAPLVGQFPASGGA
                     PPQLVGTGEYAIGVAYTHALARYRHDGFPVSTIVPPATAGEVGAVSIIAGGPNPDNAR
                     RFVDFVLSAKAEAAFSAQSLTTPLNPEVALPEGANTFADFDFIDYDAKLAGDQRDEVL
                     LRWQQVVH"
     gene            1028..2686
                     /locus_tag="PKN52_01840"
     CDS             1028..2686
                     /locus_tag="PKN52_01840"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HNQ98710.1"
                     /translation="MTYGVRSLGRLWRYDRGLLLAYGLVAVFLLVFVVAPLVRVAFEP
                     TLADWRQVFTTPRWRGASLNTLTMLVLSTGSSLLVGTLYAYAVTRARVPGAGFFSVVP
                     LFLLLTPPFVAGLAFILLLGRRGLITYQLLGLEVSIYGWHGLWLAQTLSFFPVAYLVL
                     RGAFAAIDPGLEQAARGLGASRWQVLRTVTLPLATPGLLAAALFIAIGVLGDFGNPML
                     VGGRFQVLATAVYTQLSGWASFGTSAALGLLLLLPAILLFAAQQWVQAATRERFATVG
                     GRGSSLPAPAVSPWLRWGLFAFCVLVTLFVAASHGVVLMGALTHLWGIDFTPTLEHVA
                     YVLGQFGGLGNSLRFAAAAALLCTVVAATAAYLVQRAKVPLRRGLDLATLLPAAIPGT
                     LMGVAFVLAFNQPTFRLTGTASIIVIAMAISYLPVGYRICAAAMDQLKPSLDESAANL
                     GASKLRILATITAPLVRQALAAAFVFSFVRAVGTLSTVIFLVSFDTPLASVAILNLAD
                     QGRWGRAAALASALVAVTMLSLGLLYLITGRSLKPLAESAHAGV"
     gene            2673..3905
                     /locus_tag="PKN52_01845"
     CDS             2673..3905
                     /locus_tag="PKN52_01845"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ98711.1"
                     /translation="MRASEAEPPVHVRLIGLRKTFGTAVAVEDFSLDIGRGTFTTLLG
                     PSGSGKTTVLRMLAGFVEPDAGGVLIGGIDQTGRPPNLRGTGMVFQDYALFPHMTVQA
                     NVEYGLRMQRIDKRVRRERVQSALELLGLTDLAERFPHELSGGQQQRVALGRVIVLEP
                     QVLLMDEPLSNLDAKLRVRLRAELKELQRQLGITTVYVTHDQEEALSLSDQVVVIDKG
                     RMQQVGAPEDIYRRPANRFVAEFVGQANVLPVEASATGHGEGSTADDEAPGPTARGGV
                     ATPTTSEPQDRAAKEATVWAAAAGRRLLVRLPDARRPDAGSTGTALVRPEHVRLAHPR
                     SAGGATGTGSWQCAAKVVSRGFFGAYARYWLELDGVPDPWLADVPISALDGAGSHAFA
                     PGAAVTVSLDVGAACWLW"
BASE COUNT          519 a         1534 c         1370 g          653 t
ORIGIN      
        1 gccttgccac gcgcgcatgt ccacgccgcg ccgcgcgccg cgccgcggcc ctcgcgcgcg
       61 tcctcgtttt cagcgccctc tccctcctga gcggggcctt tgcccaaggc gaggtccgcg
      121 tctactccgt catcaacgag gacgacgtca agctcctcgc ggagctgttc accgccgaga
      181 ccggcatcca ggtgacctac ctgcgcgcgg ccaccggtga gctcgtcagc cgcgtgatcg
      241 ccgagaaggg ggcaccgcaa gccgacgtgc tcttgggcgg ccccagctct cagcacatcg
      301 ccatcgccga cacgggcgct ctcgcgcagt actcgccggc ggcagcggag gcgctgcccg
      361 cttacgcggt gagccccgag gggtactgga ccggcttcta tctgaccgcc ctaggcatcg
      421 gcgtcaacga ggagcgcttc caccggctct accccgacaa gccgttcccg gccacctggg
      481 acgacctcct agacccggcg ttcaagggcg agatcgtgat gaccgacccc gtggcgtcat
      541 cgaccgcgta cctcttcgtc cagacccagc tccagcgcct cggttgggac gccggctggg
      601 cgtaccttga ggcgctcgcg cccctcgtcg gccagttccc cgccagtggc ggcgccccac
      661 cgcagctcgt cggcaccggc gagtacgcca taggcgtcgc ctacacgcac gccttggctc
      721 gttaccgcca cgacggcttc cccgtctcga ccatcgtgcc gcccgccacg gccggtgagg
      781 tcggcgccgt ctcgatcatc gcgggcgggc cgaaccccga caacgcgcgg cgcttcgtcg
      841 acttcgtcct ttccgccaag gccgaggcgg ccttctcggc gcagtcgctc accacgccgc
      901 tcaaccccga ggtggccctg cctgagggcg ccaacacctt cgccgacttc gacttcatcg
      961 actacgatgc caagctggcc ggcgaccagc gcgacgaggt cctcctgcgc tggcaacagg
     1021 tggtccattg acgtacggcg tccggtcgct cggccggctg tggcgttacg accgggggct
     1081 gctcctggcg tacggcctgg tggcggtgtt cctcctcgtc ttcgtcgtcg cgccgctcgt
     1141 gcgcgtggcg ttcgagccga cgctcgccga ttggcgccag gtcttcacga ccccgcgctg
     1201 gcgcggggcg agcctcaaca ccctcacgat gctcgtcctc tcgacgggct cgtcgctgct
     1261 ggtcggcacg ctttacgcct acgccgtgac gcgggcgcgg gtgccgggcg ccggtttctt
     1321 cagcgtcgtg ccactgttcc ttctgctcac cccgccgttc gtggccggct tggcgttcat
     1381 cctcctcttg ggcaggcgcg ggttgatcac ctaccagctc ctcgggctcg aggtgtccat
     1441 ctacggttgg cacggcctct ggctggcaca gacgctgtcg ttcttccccg ttgcctatct
     1501 cgttctcagg ggagcgttcg ccgccatcga cccgggcctc gagcaggccg cccggggctt
     1561 gggagcgagc cgctggcagg tcctgcgcac ggtcacgctg ccgctcgcga cacccggcct
     1621 gctggcggcc gcgctcttca tcgccatcgg cgtcctcggc gacttcggca accccatgct
     1681 cgtgggcggc cgcttccagg tcctcgcgac ggccgtgtac acgcagctca gcggctgggc
     1741 gagcttcggg acgagcgccg ccctcgggct gctgctgctc ctaccagcca tcctgctctt
     1801 cgccgcccag cagtgggtgc aggctgccac acgcgagcgc ttcgcgacgg tgggagggcg
     1861 cggctcgtcg ctgccggcgc ccgcggtctc tccgtggttg cgctggggac tgttcgcctt
     1921 ctgcgtgctg gtcaccctct tcgtggccgc cagtcacggc gtcgtgctca tgggcgctct
     1981 cacgcacctg tggggcatcg acttcacccc cactctcgag cacgtcgcgt acgtactggg
     2041 ccagttcggc gggctcggca acagcctgcg cttcgccgcc gccgcagcgc tcctctgcac
     2101 ggtcgtcgcg gccacggccg cctacctcgt gcagcgcgcc aaggtgccgc tccgccgcgg
     2161 cctcgacctc gccacgctcc tgcctgccgc catccccggc acgctcatgg gcgtcgcgtt
     2221 cgtcctcgct ttcaaccagc cgaccttcag gttgacgggc accgcgagca tcatcgtcat
     2281 cgccatggcg atctcctacc tgccggtcgg ctaccggatc tgcgcggccg ccatggacca
     2341 actcaagccg agcctcgacg agagcgcggc caacctgggc gcgtcgaagc tcaggatcct
     2401 ggcgacgatc acggcgccgc tcgtccgtca ggccctcgcg gccgccttcg tgttctcctt
     2461 cgtgcgggcc gtcgggacgc tgagcaccgt catcttcctc gtgtcgttcg acacgccgct
     2521 cgcctcggtc gcgatcctca acctggccga ccagggcagg tgggggcgcg ccgccgcgtt
     2581 ggccagcgcc ctggtggccg tgaccatgct gtccctcgga ctcctctacc tgatcaccgg
     2641 ccgctcgttg aagccgctgg cggagagcgc gcatgcgggc gtctgaagcc gagccgcccg
     2701 tgcacgtccg gctgatcggg ctgcgcaaga cgttcgggac ggccgtggcc gtggaggact
     2761 tctcgctcga catcgggcgg ggcaccttca cgacgctgct cggacccagc gggagcggca
     2821 agacgaccgt cctccgcatg ctcgccgggt tcgtcgagcc ggacgccggc ggcgtgctga
     2881 tcggcggcat cgatcagacg ggccgcccgc cgaacctccg cggcaccggg atggtcttcc
     2941 aggattacgc tctcttcccg cacatgaccg tacaggcgaa cgtcgagtac ggcctgcgca
     3001 tgcagcgcat cgacaagcgg gtgaggcgcg agcgggtcca gagcgccctc gagctcctgg
     3061 gcctgacgga cctggcggaa cggttccccc atgagctgtc gggtgggcag caacagcgcg
     3121 tggcgcttgg gagggtcatc gtcctggagc cacaggtcct gctgatggac gagccgctct
     3181 cgaacctcga cgccaagctg cgcgtgcgtt tgcgcgccga gctcaaggag ctgcaacggc
     3241 agctgggcat cacgacggtc tacgtcacgc acgaccagga ggaggccctg tcgctctccg
     3301 accaggtcgt ggtcatagac aaggggcgca tgcagcaggt cggcgctccg gaggacatct
     3361 accggcgacc ggccaaccgc ttcgtcgccg agttcgtcgg gcaggcgaac gtgctgccgg
     3421 tggaggccag cgctaccggg cacggcgagg ggtcgacggc ggacgacgaa gcacctgggc
     3481 ccacggcgcg cggcggcgtg gcgacaccga cgacgagcga gccccaagac cgcgccgcga
     3541 aggaggctac cgtctgggcc gccgccgccg ggcgccggct gctcgtccgc ctgccggacg
     3601 cgcggcgtcc agacgcgggc agcaccggaa cggcgctggt acgccccgag cacgtcaggt
     3661 tggcgcaccc ccgcagcgcc ggcggggcga ccgggaccgg ctcgtggcag tgcgccgcca
     3721 aggtcgtctc tcgcggcttc ttcggggcgt acgccaggta ctggctggaa ctcgacggcg
     3781 tgccggaccc atggctggcc gacgtgccca tctcggcgct cgacggggcc ggatcgcacg
     3841 cgttcgcgcc cggcgcggcg gtcacggtct ccctcgacgt cggcgcggcc tgctggctgt
     3901 ggtgaggcct gagtgacccc ggaaacgccg gcgtcaagct cgaggccggc ggagcggcgg
     3961 ggcacgcctc cgccaagcgg tgcggcggcg gactcgaacg ccgaccctca cgccttgcgc
     4021 aagtaccaca cctgctcgct cggccagtcg cgcgcccagg cgtcgctgac gaagaa
//
LOCUS       DAOQYE010000060         4054 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_22636_length_4054_cov_3.542386, whole genome shotgun sequence.
ACCESSION   DAOQYE010000060 DAOQYE010000000
VERSION     DAOQYE010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 4054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4054
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22636_length_4054_cov_3.542386"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1003
                     /locus_tag="PKN52_01850"
     CDS             <1..1003
                     /locus_tag="PKN52_01850"
                     /inference="COORDINATES: protein motif:HMM:NF015588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HNQ98712.1"
                     /translation="ADIAYAVLRYATVTADEAFLRGPGAEILIETARMWRSLGFFSER
                     DGRFHIHSVTGPDEYSAVVNDNFYTNAMARLNLERAADAAERKPDLLGTTPAEVAGWR
                     RAAAAMALPFDDRLGINAQDSEFLSRKRWDLAATPREKRPLLLHYHPLVIYRHQVLKQ
                     TDLVLAEFLLSSRFSAEQKRRDFEYYDPLTTGDSTLSAAVQSIMASEVGYQRRSYRHF
                     RRMLWTDLANLHANTADGVHVAAMGGTWLALVCGFGGLRDDAGELRFDPRLPATWSSL
                     EFRLGWRGSLFHVKLSRAAIRFRLLEGAPVPVRVRGEAFVVDGEVEVAPADQGPALD"
     gene            complement(1083..1604)
                     /locus_tag="PKN52_01855"
     CDS             complement(1083..1604)
                     /locus_tag="PKN52_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98713.1"
                     /translation="MPTLEITCESMNKAMNTPLLVMETLARLHRRGYQRLRLSAGMSP
                     NGAGWRHTIAAPEKFQRDGYLLKRPYYRGVAFGSCSEYGAPFNWASCPTRDPDLVADL
                     FVERFRGVAAAGRGEDQEYADWFAAALTVCASAGEFLNMHGEYVDAARAGCFVLLSGR
                     RVPLPPRIVPRSG"
     gene            1716..2864
                     /locus_tag="PKN52_01860"
     CDS             1716..2864
                     /locus_tag="PKN52_01860"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNQ98714.1"
                     /translation="MPRRHLLSVVRTSLNEVPVTVLTGPRQSGKTTIARELLSVESPN
                     YFDLEDPVSLARLSEPMTALQHLDGLVVIDEVQRRPDLFPVLRVLVDRGSNETRFLVL
                     GSASGELLRQSSESLAGRTRNIEIGGFDLTEVGPDAAQELWLRGGMPRSFLARSDKAS
                     LGWRSDFVRTLLERDLPQWGVRVAAAALRRFWSTLAHRHAQLWNATDLARALGVDAST
                     TRRYLDLLTDALVVRQLQPWHANLAKRQVKSPKVYVRDSGLLHQLLGIDDAQALLEHQ
                     KVGMSWEGFVLEQLLATQQHDDAWFWSVHQGAELDLLLRRGDRFYGIEIKRTDAPRLT
                     TSLRTAQSDLRLERAAIIYPGNRRYALGEHVEAVPLVELARRDGLFGP"
     gene            complement(2889..3515)
                     /locus_tag="PKN52_01865"
     CDS             complement(2889..3515)
                     /locus_tag="PKN52_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98715.1"
                     /translation="MAVTPITTQLITDHAVAAFISDRGRGRHLDPFLAAEATVAEAAQ
                     ALGLSPQRMHYWVGQMEDLGLLVRVRTELRGRSRTAVYRSSADSYSLPLDLLPTSDVE
                     TLELHFQPVWRQFLRSIAAAGRKYAEGWVVSYRRTGGLPAFHIEPTTARFADAPIANV
                     WVRLELAHGAAMELKRELESLVGRYLAQPPDSSAKPFLAHVGLVEAAE"
     gene            3714..>4054
                     /locus_tag="PKN52_01870"
     CDS             3714..>4054
                     /locus_tag="PKN52_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98716.1"
                     /translation="MRPHKLIALAVGTLLLLAACGSTNPGDPGGPGGPPGGNPFDGRL
                     IARGTGYMHTMNLSANGEWIEQIKLATGYDVPALHLAGNELYVANLNMAEPMFIDVYD
                     LSTFGTKRSFT"
BASE COUNT          713 a         1353 c         1299 g          689 t
ORIGIN      
        1 cgcagacatc gcctacgccg tgctccgcta cgccaccgtg acggcggacg aggcattcct
       61 gcgcggaccc ggcgccgaga tccttataga gacggcccgc atgtggcgct cgctgggctt
      121 cttctccgag cgcgacgggc gcttccatat ccactccgtg acgggtccgg acgagtacag
      181 cgccgtcgtt aacgacaact tctacacgaa cgccatggcg cggctgaacc tggagcgcgc
      241 cgcggacgcc gccgagcgca agcccgacct actaggcacg acaccagccg aggtcgcagg
      301 gtggcgccgc gccgccgccg ccatggcctt gccgttcgac gaccggctcg gcatcaacgc
      361 ccaggactcc gagttcctga gccgcaagcg ctgggaccta gcggcgacgc cgcgtgagaa
      421 gcgccccctc ctgctccatt accacccgtt ggtcatctac cggcatcagg tgctcaagca
      481 gacggacctc gtgctcgcgg agttcctgct gagcagccgc ttctcggccg agcagaagcg
      541 ccgcgacttc gagtactacg acccgctgac gacgggcgac tcgaccctgt cggccgccgt
      601 gcagtccatc atggcctcgg aggtcggtta ccagcggcgc tcctaccgcc acttccggcg
      661 gatgctgtgg acggacctgg cgaacctgca tgcgaacacg gcggacggcg tccacgtcgc
      721 ggcgatgggc ggcacgtggt tggcgctggt ctgcggcttc ggcggcttgc gcgacgacgc
      781 cggcgagtta cggttcgacc cacgcctgcc ggcgacgtgg agttctctcg agttcaggct
      841 ggggtggcgc ggctcgctgt ttcacgtgaa actatcgcgc gcggcgatcc ggttccggct
      901 cctggagggc gcgcccgttc ccgtgcgggt gcgcggggag gcgttcgtgg tcgacggtga
      961 ggtcgaggtg gcgcccgcgg atcaggggcc ggcgctcgac tgaagtccgg aagcaggtgt
     1021 gacctgacca agggccgtag ggtagcgctc catcttctag ccgcgatccg accgcgctgc
     1081 cgctagccgg atcgcgggac tatccgcggc ggcagaggta cgcgccggcc ggacaacaac
     1141 acgaagcaac cagcccgcgc ggcatctacg tactctccgt gcatgttcaa gaactcacct
     1201 gctgacgcac agaccgtgag cgctgccgcg aaccagtccg catactcctg atcttcgccg
     1261 cgacccgccg ccgccacacc ccggaaacgc tcgacgaaca ggtcggctac caggtcggga
     1321 tcacgcgtcg ggcagctcgc ccagttgaag ggtgcaccgt actcgctgca actgccgaag
     1381 gctactccgc gatagtacgg tctcttgaga aggtagccgt cccgctggaa cttctccggc
     1441 gccgcgatag tgtgacgcca cccggcaccg ttagggctca ttcctgcgga caggcgcaga
     1501 cgctgatagc cacgccggtg tagccgggcg agggtctcca tcactaggag cggcgtgttc
     1561 atcgccttgt tcattgactc gcaggttatc tccaacgtcg gcaagcgtgc acacccgttg
     1621 gttcaacgac cccgttgaag agcaagggac atggttagac ttatgaaatt cccgcacgtc
     1681 ctgccggaat ttcatagtat cttatccggg tgatcgtgcc gcgccgtcat cttctgagcg
     1741 tcgtcagaac gtccctgaac gaggttccgg taaccgtact aaccggcccg aggcagtccg
     1801 gaaagacaac catcgcgcgt gagcttctca gcgtggaatc gcccaactac ttcgacctgg
     1861 aggatccggt cagtcttgcc aggctgtcag agcccatgac ggcgctgcag cacctcgacg
     1921 gtctggtcgt aatagatgaa gtgcagcgtc ggccggacct cttcccggtg ctacgcgtct
     1981 tggtcgatag aggctcgaac gagacgcgct tcttggtgct cggcagcgcc agtggggaac
     2041 tcctgcggca gagctcggag agtctcgccg gccggacccg caacatcgag atcggaggct
     2101 tcgacctgac cgaagtcggc cccgacgcag cgcaggaact atggctcagg ggcgggatgc
     2161 cacgctcatt cctcgccagg agcgacaagg cgagccttgg gtggcggagc gacttcgtgc
     2221 ggacgcttct cgagcgagac ctcccgcagt ggggcgtgcg tgtggccgca gcggcgctac
     2281 ggcgcttctg gagcacgctt gctcaccgtc acgcgcaact ttggaacgcg accgacctcg
     2341 ctagggcact cggcgttgac gcttccacca cacgccgata cctcgatctg ctaacggacg
     2401 cattggtggt caggcagcta cagccttggc acgcgaacct cgccaaacgg caggtcaagt
     2461 cacccaaggt atacgtgcga gacagcggtc tgcttcacca gcttctggga atcgacgatg
     2521 cccaagcgct gctagagcac cagaaggtgg gcatgtcgtg ggaaggtttc gtcttggaac
     2581 agttgctcgc tacgcagcag catgacgatg catggttctg gtcggtgcac cagggggccg
     2641 aactcgacct cctcttgagg cgaggtgacc ggttctacgg gatcgagatc aagcgtacgg
     2701 acgcgccacg actcacgacc tcgctgcgaa cagcccaaag cgacctccga ctggagcggg
     2761 cagccatcat ctaccctggc aatcgacgtt acgcactggg agagcacgtt gaggcagttc
     2821 cgctggtcga attggcaagg cgcgacggtc tgttcggtcc gtaagtctca tctcaggtca
     2881 gcaacgacct actccgccgc ctccaccaac ccgacatgcg ccaggaacgg cttggcgctc
     2941 gaatcgggag gttgagcgag gtaccgccct accaagctct ccagctcgcg cttcagttcc
     3001 atggccgccc cgtgcgccag ctccaagcga acccacacgt tggcgatcgg cgcgtcggca
     3061 aagcgcgccg tggtcggctc gatatggaag gccggcaagc cgccggtccg gcggtagctc
     3121 accacccagc cctccgcgta cttccgtccg gccgcggcga tggaccgcag gaactgacgc
     3181 caaacgggtt ggaagtgcag ctccagggtc tcgacatcgc tcgtaggtag gaggtcgagc
     3241 ggcaggctgt acgaatcggc gctcgaccga taaacggccg tacggctccg gcctctgagt
     3301 tcggtgcgga cccgcaccag aaggccgagg tcttccatct gcccgaccca gtagtgcatg
     3361 cgttgcgggc tgagccccaa ggcctgcgcg gcctcggcca ccgtcgcctc ggcggctagg
     3421 aacgggtcga ggtgtctgcc gcggccgcgg tcgctgatga acgcagccac tgcgtggtcg
     3481 gtgatgagct gcgtggtgat aggcgtgaca gccaccagac gagcctacca ttcaactact
     3541 cacctgggtt ttttggcgtg ctctccttag catgtcatgc agagtttgcc cgtcactgcg
     3601 cctcgttcgg cgcaaggccc gggcacgtag accgcgtcgg ccgcgcgtct cgtccgccga
     3661 cccgagtcgc cggcgtcgac cgggcctacc tcggtcgcgg atgggagccc ccgatgaggc
     3721 cacacaagtt gatcgcgtta gcggtcggta ccctgctgct cctggccgcc tgcggtagca
     3781 cgaaccccgg agacccgggc gggccgggtg gtcctccggg cggcaacccg ttcgacggtc
     3841 gcctgatcgc acgaggcacc ggctacatgc acaccatgaa cctgagcgcc aacggtgaat
     3901 ggatcgagca gatcaaacta gccacgggct acgacgtgcc cgccctgcac ctggccggca
     3961 acgagttgta cgtggccaac ttgaacatgg ccgagcccat gttcatagat gtctacgacc
     4021 tgtcgacctt cggcaccaag cgcagcttca cgtg
//
LOCUS       DAOQYE010000061         4005 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_23003_length_4005_cov_2.730380, whole genome shotgun sequence.
ACCESSION   DAOQYE010000061 DAOQYE010000000
VERSION     DAOQYE010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 4005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4005
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23003_length_4005_cov_2.730380"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..768
                     /locus_tag="PKN52_01875"
     CDS             <1..768
                     /locus_tag="PKN52_01875"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HNQ98717.1"
                     /translation="EVLAEADDAPLAAARRLSVTELAEAARSSEGSIIRFCQDLGFSG
                     FQEFKLTLAVESGTPSRVDEANGEANLLAKLADHAAGTLAETATLFEQEVFEVVAARI
                     AVADRIDIYGVGASGVVAQYFAYKLLRFGLTAQAYTDLHLGSMSASNLSHAAVAIGIS
                     SSGSTIDTLRAVKVAKAAGAFTVAVTNRLKSPLAKVADRSLFASPPESPLTGGDVFAK
                     IGQLLVLEGLVRIMGEADAEFKEGVRRTAQVVADRSM"
     gene            772..1719
                     /locus_tag="PKN52_01880"
     CDS             772..1719
                     /locus_tag="PKN52_01880"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNQ98718.1"
                     /translation="MARAPVLAVDVGGTKLAAALVRGAEVLDQELVATPAKEGPEAVL
                     AELRTAVEALVGRAPETPVALGVACAGLVRAGKVRAVSRDLLPGWDDFPLVERLESAF
                     GLPVAALNDAQAAAYGEALHGAGRERGSSFFVTVSTGVGGGFVIGDRLWQGATGLAGH
                     LGHVGGGVLERVASGPALARRAAEFGHATTARELVAAAEELQPWASGLVADAAAALAT
                     ALVDIKYLLDPEVVVLGGGVGLNPVFRRALEKALQGLGEDERVLVLAAELGAAAGLVG
                     AAAWAVEGVQDRVRLALPEGSHHPPDALQVKQLMPDEPP"
     gene            complement(1642..2169)
                     /locus_tag="PKN52_01885"
     CDS             complement(1642..2169)
                     /locus_tag="PKN52_01885"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNQ98719.1"
                     /translation="MEPAAPAPSVVESVRGNEALFQHDREPAKLGEVELCMNLLRCTR
                     ADLLNVVTGLPDELLDWDPPYASFAAWARWRTVREVLTHVALTEVGYYLPAIGYAGPG
                     ARELAPLHWTEQLRLSRESTLAFLAELADADDRLRLAEGDESWSVRKVLRRLVWHELL
                     HLKSVRRVMGAFRER"
     gene            complement(2421..3821)
                     /locus_tag="PKN52_01890"
     CDS             complement(2421..3821)
                     /locus_tag="PKN52_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98720.1"
                     /translation="MRAGRAPRNTPRRALCRPSFSPLRPFDLLVTCFALCLTAGAFAE
                     PFFLPTGDEVFNTGDPRVRADAAGGLHLVYPLVAATGAAYSYCPPGCDGPEEMATVVF
                     HTDDFGAVSNALVALDSAGAPRVLLATHQSVLYGECSGDCRYGGSWSLMEIYAHDGNW
                     ELTGEAFALDQNGRPRFLMHAEQTYLGLFAPDPGTAFFSCDADCLDFGNWRRGLISEQ
                     SWLEPTFVYDARGVAHVAAVIPLEDSQLVAYLTCSVDCGSEDVDNWPGIGLGYAFYDL
                     YIEEIQPTVSMAVTSTGGVRLAFLGKDGDSRFLAYFECERECTQADGETWQALALIGN
                     ETAQGLGDGIQLVLTRDDKPRLAYTHNSNIFLGHCDDDCVGSSGSDGWGADVVETGED
                     IPADTIFLNRGCNVGYWFLRQPSVALTQSGEALVAYRAEDISAGWTPSPTDPSVRGCA
                     AGVDMTLGRLARLGGD"
BASE COUNT          586 a         1353 c         1465 g          601 t
ORIGIN      
        1 gaggtcctgg cagaagcgga tgatgctccc ctcgctgctg cgcgccgcct cagcgtcacc
       61 gagcttgccg aggcggcgcg cagcagcgag gggagcatca tccgcttctg ccaggacctc
      121 ggcttctccg ggttccagga gttcaagctg acgctcgccg tggagtccgg caccccgagc
      181 cgcgtcgacg aggcgaacgg cgaggcgaac ctcctggcca agctcgcgga ccatgctgcc
      241 ggcacccttg ccgagaccgc gacgctcttc gagcaggaag tcttcgaggt cgtcgcggcg
      301 cgcatcgccg tggccgacag gatcgatatc tatggggtcg gcgcttcagg ggtcgtcgcg
      361 cagtatttcg cctacaagct cctgcgtttc ggcctgaccg cccaggcgta cacggacctg
      421 cacctcggtt cgatgagcgc ctccaacctg tcgcacgcgg ccgtggccat cggcatctcc
      481 agctcgggtt cgaccatcga cacgctccgg gccgtcaagg tggccaaagc cgccggcgcc
      541 ttcaccgtcg cggtcaccaa ccgactgaag agccccctgg ccaaggtcgc ggaccgcagc
      601 ctcttcgcct cgccgcccga gtcgccgttg actggcggcg acgtcttcgc gaagatcgga
      661 cagctcctcg tgctggaagg cctcgtgcgg atcatgggcg aggcggacgc ggagttcaag
      721 gagggcgtgc ggcgcacggc ccaggtagtc gcggacagga gcatgtgaac cgtggcgcga
      781 gcgcccgtac tcgccgtcga cgtcggcggc accaagctgg cggcggcgct cgtgcgggga
      841 gccgaggtcc tcgaccagga gttggtagcg acgccggcga aggagggtcc tgaagcggtc
      901 ctcgcggagc tgcgaaccgc cgtggaagcg cttgtggggc gagcgcccga gaccccggtg
      961 gcgttgggcg tggcctgcgc cggtctcgtt cgcgccggca aggtacgggc cgtgagccgc
     1021 gacctgctcc cagggtggga cgacttcccg ctcgtggaga ggttggagag cgcgttcggc
     1081 ttgccggtcg ccgctctcaa cgacgctcag gccgccgcct acggggaggc gctgcacggc
     1141 gcgggccgtg agcgcggctc gtccttcttc gtcaccgtgt cgaccggcgt cggcgggggc
     1201 ttcgtgatcg gcgaccggct ctggcagggt gcgacgggcc tcgccggcca tctgggccat
     1261 gtcggcggcg gcgtacttga gcgagtggct tcgggcccgg cgttggctcg cagagcggcc
     1321 gagttcgggc atgccacgac cgcccgcgag ctggtcgccg ccgccgagga gctgcagccg
     1381 tgggcgagcg gcctcgtggc ggacgccgcg gcggcgctgg ccaccgccct ggtcgacatc
     1441 aagtacctcc tcgaccccga ggtcgtcgtc ctcggcggcg gcgttggcct gaaccccgtg
     1501 ttcaggcgcg cgctcgagaa ggcgctccag gggctgggcg aggacgagcg cgtcctcgtg
     1561 ctggccgcgg aactcggtgc ggcggccggg ctcgtcggtg cggcggcctg ggcggtcgag
     1621 ggcgtacagg accgcgttcg cctagcgctc ccggaaggct cccatcaccc gccggacgct
     1681 cttcaggtga agcagctcat gccagacgag ccgccgtaac accttgcgca ccgaccagct
     1741 ctcgtcgccc tcagcgagcc tgagccggtc gtcggcgtcg gcgagttcgg cgaggaaggc
     1801 gagggtgctc tcgcggctga gccgtagctg ctccgtccag tggagagggg cgagttctct
     1861 agcgcctggc ccggcgtagc cgattgccgg caggtagtaa cccacctctg tcagcgcgac
     1921 gtgcgtcagg acctcgcgga cggtacgcca gcgcgcccag gcggcgaacg acgcgtaggg
     1981 cgggtcccag tcgagcagct cgtctggcag gccggtcacg acgttcagca ggtccgcgcg
     2041 cgtgcacctc agcaagttca tgcagagttc cacctcgccc agcttggcag gctcacggtc
     2101 gtgctggaag agggcctcgt tgccccgcac gctctcgacc accgacggcg cgggggccgc
     2161 tggctccacg acgtccggct gggtggttgg ctcggtgcac gtggcgagcc caccaccagc
     2221 cgcggaccca ggcggaacgt cgaggccgtg accgaccaac caagccctat gactcgcgag
     2281 ccgctcagga gcgcggctca agacccccgc acgcgtggga cgccacgtcg cgaaccccag
     2341 gtaccgaagg tcccaggcct gccagccgct ctcgtcgccg gtcatttcct ggagccacag
     2401 catcgcgccc gcggggtccg tcagtcgccg ccgagccgcg ccagtcgccc cagggtcatg
     2461 tcgacgccgg cggcgcagcc gcgtacgctc gggtccgtcg gcgagggggt ccagccggcg
     2521 ctgatgtcct cggcgcggta ggcgacgagc gcctcgcccg actgggtcag ggctaccgac
     2581 ggctgcctga ggaaccagta gccgacgttg cagccgcggt tgaggaagat ggtgtcggcc
     2641 gggatgtcct cgccggtctc gaccacgtcg gcgccccagc cgtccgagcc ggagcttccg
     2701 acgcagtcat cgtcgcagtg cccgaggaag atgttcgagt tgtgcgtgta cgccagccgg
     2761 ggcttgtcgt cgcgcgtgag gacgagctgg atgccgtccc ccaagccctg ggcggtctcg
     2821 ttaccgatga gcgccagcgc ctgccaagtc tcgccgtccg cctgggtgca ctcgcgctcg
     2881 cactcgaagt acgccaggaa gcggctgtcg ccgtccttgc cgaggaaggc gaggcgcacg
     2941 ccgcccgtgc tcgtgacggc catgctcacg gtcggttgga tctcctcgat gtacaggtcg
     3001 tagaaagcgt agccgaggcc gatgccgggc cagttgtcga cgtcctcgga gccgcagtcg
     3061 acgctgcacg tgaggtacgc gacgagctgc gagtcctcta gtgggatgac ggcggcgacg
     3121 tgcgccacgc cgcgggcgtc gtagacgaac gtcggctcca accagctctg ctcgctgatg
     3181 agaccgcggc gccagttgcc gaagtcgagg cagtcggcgt cgcaggagaa gaaggcggtg
     3241 cccgggtcgg gcgcgaagag gccaaggtag gtctgctcgg cgtgcatgag gaagcgcggg
     3301 cgaccgttct ggtcgagcgc gaacgcttcg cccgtcagtt cccagttgcc gtcgtgggcg
     3361 tagatctcca tgagggacca cgagccgccg tagcggcagt cgccgctgca ttcgccgtag
     3421 aggaccgact ggtgcgtggc gaggaggacc ctcggagcgc ccgcgctgtc tagggccacg
     3481 agcgcgttgc tgacggcgcc gaagtcgtcc gtgtggaaca cgacggtagc catctcctcg
     3541 gggccgtcgc agcccggcgg gcagtaggag taggcggcgc cggtagcggc gacgagcgga
     3601 tagaccaggt ggaggccacc cgcggcgtcg gcgcgcacgc gcggatcccc cgtgttgaag
     3661 acctcgtcgc ctgtcggcag gaagaacggc tcggcgaagg ctcccgccgt caggcatagg
     3721 gcgaagcagg tgaccagcag atcgaacggg cgcaggggtg agaaggaggg tctacaaagg
     3781 gctctacgtg gtgtgttacg tggggctctt ccggctctca tagccgacca ttatggcgtg
     3841 gcctcacctt cgcggctcgc gcatccgtcc tagctcaaga acctccgcgc ctcgtctggc
     3901 gcgggcagcc ggcaagcgtc cgtgcgcccg aaccagctgt aacggcgcct ggcgacgaag
     3961 tcgtatacgg catcgcgcag ggaggtggga aggatcgagc atgcg
//
LOCUS       DAOQYE010000062         4003 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_23012_length_4003_cov_3.175785, whole genome shotgun sequence.
ACCESSION   DAOQYE010000062 DAOQYE010000000
VERSION     DAOQYE010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 4003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4003
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23012_length_4003_cov_3.175785"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            122..1213
                     /locus_tag="PKN52_01895"
     CDS             122..1213
                     /locus_tag="PKN52_01895"
                     /inference="COORDINATES: protein motif:HMM:NF013345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-TA family PLP-dependent enzyme"
                     /protein_id="HNQ98721.1"
                     /translation="MSPSASTMPTAVRIETLDTPCLVVDLDVVERNIRRLQEYLDAHG
                     IANRPHIKTHKLPLLARWQVEAGARGITVQSVGEAEVMAAAGLDDILVTYDVMGADKV
                     RRLAQVASMATVRVAVDNEVALDAVAAAAALAGRTIGVLVEFECGKRRQGVVTPEEAV
                     ALARSAAGRPNVEFLGLLTYPNSDVVPGFVTRTRELLGEAGLELRVVSVGGTPGVWRS
                     HVPGVATEHRAGTYVYNDRNSVAAGSATLEDCALHVHVTLVSKPTAERGVIDAGSKTL
                     TSDLFRGDPAGGYGHVLEYPGAVVWQLSEEHGAVDFSACADKPVVGERLRVVPNHVCP
                     VSNLHDEVYAHRGGVVCAVLPVAARGKTR"
     gene            1210..1731
                     /locus_tag="PKN52_01900"
     CDS             1210..1731
                     /locus_tag="PKN52_01900"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNQ98722.1"
                     /translation="MSAPGPAGGGPAARVRERASGSSASVVRPSAEHRAAWDALYAGY
                     ADFYRKAQTQEMRDRVWGWILDPASEVECLLALDAGGKPVGLAHFREFARPLDASKGG
                     YLDDLFVAPAARGSGAAQALFGALRDEARHRGWTVVRWITADDNYRARAVYDRVATRT
                     PWLTYDLAPRSDG"
     gene            1959..3131
                     /locus_tag="PKN52_01905"
     CDS             1959..3131
                     /locus_tag="PKN52_01905"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNQ98723.1"
                     /translation="MQVSMLSSWRLARLALLGLVLGSLAWAQDASSLAVDSFTSDFSA
                     MQALTGLAAQGQGKIGVLLPETTTSARYTAFDEPFLKQAFHAAGVPDADVIVTNAQGS
                     ESTQLTQAQAAITQGATVLLVDPISSGVGASIEQYALDHGVKVIDYDRLTLGGSRAYY
                     VSFDNVQVGKLIGDGFVSCVQAWNVSDPHVLIMNGAPTDNNATLFAQGYHSVIDPLVS
                     AGTYTLVGEPAGTWDPAQARTTFEGQYTAHSDINAVVTPNDDNANAVIAYLKTLGVPA
                     KTFPTTGQDATVQGLQNVLAGYQCGTVYKPINLEAQAAAALAVFLRAGVTPPETLVNG
                     VTRDSEAGSDVQSVLLTPIWVTPANMAATVVKDGFQKAADLCAGFADECGAAGIGQ"
     gene            3250..3969
                     /locus_tag="PKN52_01910"
     CDS             3250..3969
                     /locus_tag="PKN52_01910"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNQ98724.1"
                     /translation="MAAEPLMRLAGVHKSFGAVHALRGVDLTVPSAAVTALVGDNGAG
                     KSVTVKVVAGTHLPDEGQMFWEGRPVHPRTPREAAALGIEVVYQDLALCDNLDVVQNM
                     FLGREAVRRGFLDEDAMERAAMRTLADLQVTTIQSVRLPVASLSGGQRQSVAVAKAVM
                     WNSKLVILDEPTAALGVAQTRQVLNLVRRLAERASGSSSSPTTSRTSSPWPTGSPSCA
                     SAGWSARGRPPTPTCRRWWSS"
BASE COUNT          603 a         1462 c         1401 g          537 t
ORIGIN      
        1 cgtgccctat gccgacgccg gggaacaggc tccccttcgc cgtcacggca ggcgagcggc
       61 tggctcgggg ttaagcaggc gcggacctgc gcggcgtacg cttgggtggc caaggaggaa
      121 catgagcccc tctgcatcaa ccatgccgac cgccgtgcgc atcgagacgc tcgacacccc
      181 gtgcctggtc gtggacctcg acgtggtgga gcgcaacatc cggcgcctgc aggagtacct
      241 cgacgcgcac gggatagcca accgccccca catcaagacg cacaagctcc cgctcctcgc
      301 gcgctggcag gtcgaggcgg gcgcgcgcgg cataaccgtg cagagcgtcg gcgaggcgga
      361 ggtcatggcc gcggccgggc tggacgacat cctcgtcacc tacgacgtga tgggcgccga
      421 caaggtgcgg cgcctggcac aagtggcgag catggcgacc gtgcgggtgg cggtggacaa
      481 cgaggtcgcc ttggacgccg tcgccgctgc tgccgcgctc gcggggcgca cgatcggcgt
      541 gctcgtcgag ttcgagtgcg gcaagaggcg ccagggcgtc gtgacgccgg aggaggccgt
      601 cgcgctggcg cgctccgccg ccggccggcc gaacgtcgag ttcctcggcc tcctcacgta
      661 ccccaactcc gacgtggtgc ctggcttcgt gactcggacc cgcgagctgc tcggggaggc
      721 gggcctcgaa ctgcgggtcg tatccgtagg cggcacgccg ggcgtgtggc ggtcgcacgt
      781 gccgggcgtg gcgacggagc accgcgcggg cacttacgtc tacaacgatc gcaacagcgt
      841 cgccgccggc tcggccacgc tggaagattg cgccctgcac gtccacgtga cgctggtgag
      901 caagccgacg gccgagcgcg gcgtgataga cgccggctcc aagaccctca cgagcgacct
      961 cttccgcggc gacccggccg gcggctacgg ccacgtcctc gagtacccag gggccgtcgt
     1021 gtggcagctc tccgaggagc acggggccgt cgacttctcg gcctgcgccg acaagccggt
     1081 cgtgggcgag cgcctgaggg tggtgccgaa ccacgtgtgc ccggtgtcca acctgcacga
     1141 cgaggtgtac gcgcaccgcg gcggcgtggt gtgcgccgtg ctgccggtcg ccgcgcgggg
     1201 gaagacgcga tgagcgcccc gggcccggct ggtggcggac ccgccgcgcg ggtccgggag
     1261 cgggcgagcg gctcgagcgc gagcgtcgtg cgcccctccg ccgagcaccg cgcggcctgg
     1321 gacgcgctgt acgcgggtta cgcggacttc taccgcaaag cccagacgca ggagatgcgc
     1381 gaccgggtct ggggctggat cctcgacccg gcgagcgagg tcgagtgcct cctcgccctg
     1441 gacgccggcg ggaagcccgt cggcctcgcg cacttccgcg agttcgcccg ccccctcgac
     1501 gcgtcgaagg gcggttacct cgacgacctg ttcgtcgcgc ccgcggcccg cggcagcggc
     1561 gcagcgcagg cgctcttcgg ggccctgcgc gacgaggcgc gccaccgcgg ttggaccgtc
     1621 gtgcgctgga tcaccgcgga cgacaactac cgtgcgcgcg ccgtgtacga ccgggtagcc
     1681 acgcgcacgc cctggttgac gtacgacctc gcgccccgca gtgacggttg aggcaccttc
     1741 tcgccaaccg gcggcgctac gctgacaggc gcttcgggtc gtgccgcctc gtcgccctga
     1801 tggcgggcgg ccacgcccga caggcgcgct gccagtgccg tcgctccaag gcagcggacc
     1861 ctggcctagc cagcagcccg ccgcacccac cgaagcactg aagccgacac cggccggggc
     1921 cccgatgacg tggtccgtca gggagaaagc cggcgatcat gcaagtaagc atgctgtcat
     1981 cgtggcggct cgcccgcctg gcgctgctcg gcctggtcct tggtagcctc gcctgggcac
     2041 aagacgcttc gtcgctagcg gtagattcgt tcaccagcga cttctccgcc atgcaagccc
     2101 tcaccgggtt ggcggcgcag ggccagggga agatcggcgt gctcctgccc gagacgacga
     2161 cctcggcgag gtacacggcg ttcgacgagc cgttcctcaa gcaggcgttc cacgcggcgg
     2221 gcgtgcctga cgcggacgtg atcgtcacga acgcccaagg cagcgagtcg actcagctca
     2281 cgcaggcgca ggccgccatc acccagggcg ccaccgtgct cctcgtcgac cccatctcct
     2341 cgggcgtcgg cgcctcgatc gagcagtacg cgctcgacca cggggtgaag gtcatcgact
     2401 acgaccgcct cacgctcggc gggtcgcgcg cctactacgt gagcttcgac aacgtccagg
     2461 tcggcaagct catcggcgac gggttcgtct cgtgcgtgca ggcctggaac gtctccgacc
     2521 cgcacgtcct gatcatgaac ggcgcgccga cggacaacaa cgccacgctc ttcgcgcagg
     2581 gctaccactc cgtcatcgac ccgctcgtca gcgcaggcac ttacacgctc gtcggcgagc
     2641 cggccggcac gtgggacccg gcgcaggccc gcaccacgtt cgagggccag tacacggcgc
     2701 actccgacat caacgcggtc gtgaccccca acgacgacaa cgccaacgcc gtgatcgcgt
     2761 acctcaagac gctcggcgtg ccggccaaga ccttcccgac gaccggtcag gatgccacgg
     2821 tgcagggtct ccagaacgtc ctcgccggct accagtgcgg cacggtctac aagcccatca
     2881 acctcgaggc ccaagccgcc gcggccctcg ccgtcttcct gcgcgccggg gtcactccgc
     2941 ccgaaacgct cgtgaacggc gtcacgcggg actcggaggc cggctccgac gtgcagtccg
     3001 tcctcctcac gcccatctgg gtgacgcccg cgaacatggc cgccacggtc gtgaaggacg
     3061 gcttccagaa ggcggccgac ctctgcgccg gcttcgccga cgagtgcggc gccgccggga
     3121 tcgggcagta ggaccgcggc gtggcccgga gcacgaccac gacgtggcgg gaagcgggac
     3181 cgcggcgtga atgacgcacg tgctcggcac tacgagccga ctcggccaca ggctcggcag
     3241 cctcaggggg tggctgccga gccgctcatg cgactcgcgg gcgttcacaa gagcttcggc
     3301 gccgtgcacg cgttgcgcgg cgtcgacctc accgtcccga gcgccgcggt gacggccctg
     3361 gtaggcgaca acggcgcggg caagtccgtc accgtcaagg tcgtcgccgg cacgcacctg
     3421 cccgacgagg ggcagatgtt ctgggaaggc cgccccgtcc acccccggac gccgcgcgag
     3481 gcggccgccc tcggcatcga ggtcgtctac caggacctcg cgctatgcga caacctcgac
     3541 gtggtgcaga acatgttcct cggccgcgag gcggtgaggc gcggcttcct cgacgaggac
     3601 gcgatggagc gggcggccat gcgcacgctc gccgacctgc aggtgacgac catccagtcc
     3661 gtgcgcctgc cggtcgcctc gctgtccggc ggccaacgcc agtccgtcgc ggtcgcgaaa
     3721 gccgtgatgt ggaactccaa gctcgtcatc ctcgacgagc cgacggccgc cctcggcgtc
     3781 gcccagacgc gtcaggtgct gaaccttgtc aggcgcctgg ccgaacgggc ctcggggtcc
     3841 tcctcatctc ccacaacctc acggacgtct tcgccgtggc cgaccggatc gccgtcatgc
     3901 gcctcggcag ggtggtcagc gaggggccga ccgccgacac cgacgtgcag acggtggtgg
     3961 agctcatgac cacggggaag gcgcgggcag catgacgacc aac
//
LOCUS       DAOQYE010000063         3980 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_23204_length_3980_cov_2.543694, whole genome shotgun sequence.
ACCESSION   DAOQYE010000063 DAOQYE010000000
VERSION     DAOQYE010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 3980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3980
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23204_length_3980_cov_2.543694"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1138
                     /gene="dctP"
                     /locus_tag="PKN52_01915"
     CDS             <1..1138
                     /gene="dctP"
                     /locus_tag="PKN52_01915"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HNQ98725.1"
                     /translation="TTTLAGTAAQAGRRVLLEVEMNRRNFMKKAGIAVAGASISPLMF
                     ANAQRSKFSFEMVTSWPTSLDTLYGGAESVARYIEEASSGDITVKVYPAGAQIGALEV
                     YDAVSTGAFAMAHTAPYYFINKNPAHGFFTTVPFGMDSQQFNAWMYSGNGDALHQELL
                     APDDMVAFPSGNTGAQTGGWFKREVHTVADLQGLSMRFPGFGGQVMGRVGMNVQNIPG
                     GELFLALDTGVVDAADWVGPYDDQILGLNKAAPYYYFPSWAEPGPGVCTYMNKGEFES
                     LPTDLQAVIRDACARVNVEMLSNYDTKNYRALQELVASGTQIRLFSDEILKAFAEATE
                     AIHAENAANNAAYQRILDDYRAFLDNVRAWTKITYNAFNEFIFQ"
     gene            complement(1297..2670)
                     /locus_tag="PKN52_01920"
     CDS             complement(1297..2670)
                     /locus_tag="PKN52_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HNQ98726.1"
                     /translation="MEYLAFYMFGAAVVLLLIGFPVAFSLGGTAVLFTLIGSDYLPGL
                     GIDLPWEPLFRFARLNILPQRIYGTIMENYTLVAVPFFVFMGVMLERSGLAEELLETM
                     GILFGRLRGGLAISVVVVGALLAASTGVVGASVVTMGILALPVMLKYNYDKSLSAGVI
                     AASGTLGQIIPPSIVLVILGDQLGISVGQLFLGSFIPGFVLAGLFVVYVAVVAYFRPK
                     VAPALPEEVRRYQPRVLALKVVRSLIPPLFLILVVLGSIFFGIATPTEAGAAGAIGAM
                     LLALINRRLTLKNLTDTMRQTVQLTSMVFIILVGATAFSMVFTALRGDTLVDQFLVNL
                     PGGQWGFLFFTMLTIFLLGFFIDFIEITFIVVPFIAPIAAHFFGQDMMLWFGVVIAMN
                     LQSSFLTPPFGFALFYLKGVAPPEIRTDHIYRGIVPFVIIQVAALVLLIVFPALVNWL
                     PSVSNAQ"
     gene            complement(2670..3245)
                     /locus_tag="PKN52_01925"
     CDS             complement(2670..3245)
                     /locus_tag="PKN52_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HNQ98727.1"
                     /translation="MKAALLVARAVDAFTGAIAVVMQWLSLLMVLLGAYNVVTRYVGR
                     AIGVSLGGSIYTVLQTYAFDLLFLLAAGWVLRTDGHVRVDIVYSRLGPRAKAWVDLFG
                     TVFFLFPFCYMGFTLSLPYVGRSWQHLEVNVNAGNLPIYLMKTVIPVAFALLALQGIS
                     QVIKLVAFLRGKGPMAEAAARQEQPAQHGAG"
     gene            complement(3407..>3980)
                     /locus_tag="PKN52_01930"
     CDS             complement(3407..>3980)
                     /locus_tag="PKN52_01930"
                     /inference="COORDINATES: protein motif:HMM:TIGR02607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HNQ98728.1"
                     /translation="STEHYDVIIIGSGAGGGTLAYRLAPTGKKILLIERGEFVPREKE
                     NWDSHAVVVENRYHTNEAWLDKKGKEFHAGTHYYVGGNTKFYGAALIRLRKEDFGDLQ
                     HHDGVSPAWPIDYDTLEQYYTEAEKLYFVHGKRGVTPDTALRLARLFGTTPEFWLNGQ
                     LSWDLWHARRAHGAALLDSIEPLGGHQLPS"
BASE COUNT          795 a         1276 c         1279 g          630 t
ORIGIN      
        1 taccacaacc ctagccggca cggccgctca ggcgggccgg cgcgttctgt tggaggtcga
       61 gatgaaccgt cggaacttca tgaagaaggc tggcatagct gtggccggtg cgagcatcag
      121 tccgttgatg ttcgcgaacg cccagaggtc caagttcagc ttcgagatgg tgacctcgtg
      181 gccaacgtcg ctcgacacgc tctacggcgg cgcggagagc gtggcgcgct acatcgagga
      241 ggcttccagc ggcgatatca ccgtgaaggt ctacccagcc ggcgcccaga tcggcgcgct
      301 cgaggtctac gatgccgtct ccaccggggc cttcgccatg gctcacaccg cgccctacta
      361 cttcatcaac aagaacccgg cccacggctt cttcacgacc gtgccgttcg gcatggactc
      421 ccagcagttc aacgcctgga tgtactcggg caacggcgac gcgctgcacc aggagctgct
      481 ggcacccgac gacatggtgg ccttcccctc gggcaacacc ggggcgcaga ccggcggctg
      541 gttcaagcgc gaagtccaca cggtcgccga cctgcagggg ctgtccatgc gcttccccgg
      601 cttcggcggc caggtcatgg gtcgcgtcgg catgaacgtc cagaacatcc ccggcggcga
      661 gctcttcctc gcgctcgaca cgggcgtggt agacgccgcc gactgggtcg gaccgtacga
      721 cgaccagatc ctgggcctga acaaggccgc cccgtactac tacttcccca gctgggcgga
      781 gcccggcccc ggcgtctgca cctacatgaa caagggcgag ttcgagagcc tgcccacgga
      841 cctgcaggcg gtcatacgtg acgcatgcgc gcgcgtgaac gtcgagatgc tctccaacta
      901 cgacacgaag aactaccggg cgcttcagga gctggtcgcg tcgggcacgc agatccgctt
      961 gttctcggat gagatcctca aggccttcgc ggaggcaacg gaagcgatcc acgcggagaa
     1021 cgcggccaac aacgccgcct atcagcgcat cctcgacgac taccgcgcct tcctggacaa
     1081 cgtacgcgcc tggacgaaga tcacctacaa cgcgttcaac gagttcatct tccagtgacg
     1141 tcgggagcgt ccacaacagg cgtccgaccg cgcatgtggc gccaggcgag cggcggcgct
     1201 gcctgagcta gccgtagcgc agccacccga taacggtgcg tgggcggcct cttcgaggat
     1261 cgccccacgt tttggctgac ggctatacca agcggctcac tgggcgttcg agaccgaagg
     1321 cagccagttg accaacgccg ggaagacgat cagcagtacc agcgcggcca cctggatgat
     1381 cacgaacggg acgatgccgc ggtagatgtg gtccgttctg atctcgggcg gcgccacgcc
     1441 cttcaggtag aagagcgcga aaccgaaagg cggcgtgagg aacgagctct gcaggttcat
     1501 ggcgatgacg acgccgaacc acagcatcat gtcctgcccg aagaagtgcg cggcgatggg
     1561 cgctatgaac ggcacgacga tgaacgtgat ctcgatgaag tcgatgaaga accccagcag
     1621 gaagatcgtc agcatcgtga agaacaggaa gccccactgg ccgccgggca ggttcacgag
     1681 gaactggtct acgagcgtgt cgccgcgtag ggcggtgaag accatgctga acgccgtggc
     1741 gccgaccagg attatgaaca ccatgctcgt cagttgcacg gtctgccgca tggtgtccgt
     1801 caggttcttg agggtcaggc gccggttgat cagcgccaag agcatggcgc cgatggcccc
     1861 ggccgcgccg gcctctgtcg gcgtggcgat gccgaagaag atggagccga gcacgaccag
     1921 gatgaggaac agcggcggga tgaggctccg gacgaccttc agcgccagca cgcgcggctg
     1981 gtagcgccgc acctcctcgg gcagggccgg agcgacctta gggcggaagt aggcgaccac
     2041 ggccacgtac acgacgaaca gccccgccag aacgaacccg ggtatgaacg accccaggaa
     2101 cagctgccca acgctgatgc ccaactggtc accgagtatg accagcacga tcgaaggcgg
     2161 gatgatctgc ccgagcgtgc ctgacgcggc gatcacgccg gccgagagcg acttgtcgta
     2221 gttgtacttc agcatgaccg gcagcgcgag gatgcccatg gtcacgaccg aagcgccgac
     2281 cacgccggtc gaggcggcca ggagcgcccc gaccaccacc accgagatgg cgagtccccc
     2341 gcgcaagcgt ccgaagagga tgcccatggt ctccaggagc tcctcggcga ggccggagcg
     2401 ctccagcatg acgcccatga acacgaagaa cggcaccgcc acgagcgtgt agttctccat
     2461 gatggtgccg tagatgcgct ggggcaggat gttcaggcgt gcgaagcgga agaggggctc
     2521 ccacggcaga tcgatgccca ggcccgggag gtagtcgcta ccgatcagcg tgaacaggac
     2581 cgccgtgccg ccgaggctga aggccacagg gaagccgatg aggagcagta ccacggccgc
     2641 cccgaacatg tagaacgcca ggtactccat cagcccgccc catgctgtgc cggctgctct
     2701 tgccgggcgg cggcctcagc catcggcccc ttgcctcgca ggaacgcgac gagcttgatc
     2761 acctggctga tcccttgcag cgcgaggagg gcgaaggcga ccgggatcac ggttttcatg
     2821 aggtagatgg gcaggttgcc ggcgttgacg ttcacctcga ggtgctgcca cgaccggcca
     2881 acgtaaggga ggctgagcgt gaagcccatg tagcagaacg ggaagaggaa gaagaccgtg
     2941 ccgaagaggt cgacccatgc cttggccctc ggcccgagac gggagtagac gatatcgacc
     3001 cgcacatggc cgtcggtcct cagcacccac ccggcggcga ggaggaagag aaggtcgaac
     3061 gcgtacgtct gcagcaccgt gtagatcgaa ccacctagcg ataccccgat agcacgaccc
     3121 acgtagcgcg tgacgacgtt gtaggcgccc aacaacacca tcaggagaga cagccactgc
     3181 atcacgacgg cgatggcgcc tgtgaatgcg tcgaccgccc gggcaacgag caatgcggcc
     3241 ttcaaatcgc gctcccttcc gcggatcgct aggttgcggg tttcgttttc gcgtaatggt
     3301 actgctggcg tcggggagcg tcaacgtgac cccagccccc cgtaacaacc acttctgagt
     3361 tcacgcgtga cggcttgagc cacgcgctgc agcgtggccg tgagcctcaa gacggtagct
     3421 ggtggccacc aaggggttct atcgagtcca gcaaggctgc gccatgtgcc cgcctggcgt
     3481 gccagagatc ccaagatagc tggccgttca gccagaactc aggagtagta ccgaacagac
     3541 gcgcaaggcg tagggcggta tccggcgtca cgccgcgttt gccgtgcaca aagtaaagtt
     3601 tttccgcctc ggtgtagtac tgctccaatg tgtcgtagtc gatgggccac gccggcgata
     3661 cgccgtcgtg atgctgcagg tcgccaaagt cttccttgcg caggcgaatc agcgccgcgc
     3721 cgtagaactt ggtgttacct ccgacgtagt aatgcgtgcc cgcgtggaac tccttgccct
     3781 ttttgtccag ccacgcttca tttgtgtggt accggttttc gaccacgacc gcgtgactgt
     3841 cccagttttc cttctctcgc ggtacgaact cgccgcgttc gatgagcaag atcttcttgc
     3901 ccgtcggcgc gagacgatac gccagcgtgc cgccgcccgc gccggagccg atgatgatta
     3961 cgtcgtaatg ttcagtactc
//
LOCUS       DAOQYE010000064         3977 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_23230_length_3977_cov_3.075472, whole genome shotgun sequence.
ACCESSION   DAOQYE010000064 DAOQYE010000000
VERSION     DAOQYE010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 3977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3977
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23230_length_3977_cov_3.075472"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..798)
                     /locus_tag="PKN52_01935"
     CDS             complement(<1..798)
                     /locus_tag="PKN52_01935"
                     /inference="COORDINATES: protein motif:HMM:NF017183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HNQ98729.1"
                     /translation="MLSRQTDAARRDARSAPPKRLTAPAVVALLVLVLASGCAGDMAT
                     RGEALRLVGQDLPAAVLMEAYEGQLHAVGGLRPYTFTLTDGSLPEGIALQNGVLRGVA
                     TETGTFEISVEVSDANLSRVSQSYTLRVSAPPPPRFVLVAPQTEIRAPATLRVRLEDA
                     RLATAARTKVTWDPAEYELVGEPQAARRDVALLWRGAPGELQVDLAALSSTLSGAVEL
                     YSFTLAPLTPPVTVLVEYEAEVLTASGDPARQHEFLTGSSGRRAATRR"
     gene            984..3770
                     /locus_tag="PKN52_01940"
     CDS             984..3770
                     /locus_tag="PKN52_01940"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNQ98730.1"
                     /translation="MNDASPFLTTRAARRRYALPAAELLAQAGVARPELATPAHWAER
                     VSMARRARDGHPRLESPLSAGTLSAAAALHDGMHAVMTSPGLNGAGQAALDRAWRRRE
                     REDGREGAAAYEAAFAEAYVAAGLPETSSPPYLTGAQVAERTWLIARAIDNPALTATH
                     ELLSAPLGAEGPWGATDAAVSEALATVGAPDRGTGADGDGDAVAEAIPDAMAGSVALA
                     NQAGSLLDVLLAPMRLEPTSLVGQLRLAARLWAPYLTGARGVTERLLRAVDLLAEESK
                     KAPQFDPGPPPPPPEPVVVEEASGAARYTPDKAWMPEVAMVAKSTYVWLAQLSRTYGS
                     EIRRLDEVPEAALSALRDDGFNALWLIGVWERSQASRNLKRARGQPDAGASAYSLFDY
                     VVADDLGGEPALAALSGRAAAVGLRLASDMVPNHTGLDSSWMVEHPDWFVQLSEPPYP
                     GYRFTGPDLAAGGPIEVRVEDGYFDGTDAAVVFERKDGATGERRYVYHGNDGTTMPWN
                     DTAQLDYLRADVREAVMRTVEEVARRFPIIRFDAAMTLAKKHVQRLWHPLPGEGGAVP
                     SRARFAMGQEEFERRMPREFWRELVDRMAERAPDTLLLAEAFWLLEGYFVRELGMHRV
                     YNSAFMHMLMREENGAYRRLLKSVLAFDPRVLQRYVNFMSNPDEETAREQFGTGDKYF
                     GVATLLATLPGLPMFGHGQVEGLQEKYGMEYLAPKLDEWPEPWLVERHRREVAPLLRA
                     RGTFAGADAFRLFDLIGEHGVVEDAFVFVNRGQGGAHGAVLVAYNNSPRAVEGRVRVS
                     APYADVMRGGGLRSDTVVAALALREGGAGQVAFTTFATGLRTRTVRIAELKREGLWLQ
                     LGPYEARVELVSQTDDGPGEPAEVPALPAERARRLGTAFGLDPRLAGPRGVAGRRATK
                     RRRSSGARPPRRRR"
BASE COUNT          555 a         1400 c         1494 g          528 t
ORIGIN      
        1 gcgccgtgtc gcggcgcggc ggccgctcga tccggtaagg aactcgtgct gtctggccgg
       61 gtccccgctg gcggtcagca cctccgcctc gtactcgacg aggacggtca cggggggcgt
      121 gagcggggcg agcgtgaagg agtagagctc gacagcgccg ctcagcgtgg agctaagcgc
      181 ggccagatcg acctgcaact caccgggtgc gccgcgccag aggagcgcga cgtccctgcg
      241 cgcggcctgc ggctctccca cgagctcgta ctcggccggg tcccacgtca ccttggtgcg
      301 cgccgccgtc gccaagcgcg cgtcctccag gcgaaccctc agcgtcgcgg gcgccctgat
      361 ctcggtctga ggcgccacca gcacgaaacg cggtggtggc ggagcgctta cccgcagggt
      421 gtagctctga ctcacgcggg agaggttggc gtcggagacc tcgaccgaga tctcgaaggt
      481 gccggtctcg gtggcgacgc cgcgcagcac gccgttctgc aacgcgatgc cctcgggcag
      541 cgagccgtct gtcagggtga acgtgtacgg cctgaggcca ccgacggcgt ggagctgccc
      601 ctcgtacgcc tccatgagga cggcggccgg caggtcctgt cctacgagcc gcagggcctc
      661 gcctcgcgtg gccatgtcgc cggcgcagcc ggaggcgagg acgaggacca ggagcgcgac
      721 gaccgccggg gccgtcaggc gcttgggcgg cgctgagcgc gcgtcgcgcc gggcggcgtc
      781 ggtctgcctg cttagcatgg ctgcgattgt accgaccaag ggatgagagg agccctcgcc
      841 gcccgcgggc gccgcccgca ggcgccgccc gcaggcgccg catggttacg ctcgcgtggc
      901 cggccggctt ggctggccgg cgtggtcgcg ccagcgggtg ttgggcccgc agggcgctcc
      961 gtggcgcgtg atagagtcga gccttgaacg acgcaagccc gttcctgacg acgcgcgcgg
     1021 cacggaggcg ctacgccctg ccggccgcag agttgctcgc ccaggctggg gtggcgcgcc
     1081 ccgaactcgc cacgcccgcg cactgggccg agcgggtctc catggcgcgg cgcgcccgag
     1141 acgggcatcc tcgtctcgag tcgccgctca gcgcgggcac gctgagcgcc gccgccgccc
     1201 tccatgacgg catgcatgcc gtcatgacct cgccgggcct gaacggagcg ggtcaggccg
     1261 cgctcgaccg ggcatggaga cgccgcgagc gcgaggacgg ccgggaaggc gccgcggctt
     1321 acgaggcggc cttcgcggaa gcctacgtgg ccgcggggtt gccggagacc tcgtccccac
     1381 cctatctcac gggcgcccag gtcgcggagc gcacctggct catcgcgcgc gccatcgaca
     1441 accccgcgct caccgcgacg catgagctgc tgagcgcgcc cctcggcgcg gaagggccat
     1501 ggggggcgac ggacgcggcg gtctcggagg ccctggcgac ggtcggcgcc ccggaccgcg
     1561 ggacgggggc ggacggtgat ggcgacgccg tagccgaggc catccccgac gccatggccg
     1621 ggtcggtcgc cctggccaac caggcaggga gcctgctcga cgtcctcctc gcgcctatgc
     1681 ggctcgagcc cacttcgctc gtcggtcagc tgcgcctggc cgcgcgactg tgggcgccct
     1741 acctgaccgg cgcgcgcggg gtgacggaac gcctgctcag ggccgttgac ctcctggcgg
     1801 aggagagcaa gaaggcgccc cagttcgacc ccggtccgcc cccgccgccg cccgagcccg
     1861 tcgtggtcga ggaagcgtcc ggggccgccc gttacacccc cgacaaggcc tggatgcccg
     1921 aggtcgcgat ggtggccaag agcacctacg tctggctggc gcagctgtcg cggacctatg
     1981 gaagtgagat ccgccgcctc gacgaggtgc cggaggccgc cctgagcgcg ctgcgtgacg
     2041 acggcttcaa cgcgctatgg ctcatcggcg tgtgggagcg gagccaggca tcacgcaacc
     2101 tcaagcgcgc gcgcggccag ccagacgcgg gcgccagcgc ctactcgctg ttcgactacg
     2161 tcgtggcaga cgacctgggc ggcgagccgg cgctggcggc gctgagcggc cgggccgccg
     2221 ccgtcggcct gcgcttggcg agcgacatgg tgcccaacca caccggcctc gactccagct
     2281 ggatggtcga gcaccccgac tggttcgtgc agctctcgga accgccctac ccgggctacc
     2341 ggttcaccgg cccggacctc gccgctggcg ggcccatcga ggtgcgggtc gaagacggct
     2401 acttcgacgg caccgacgcc gcggtcgtgt tcgagcgcaa ggacggggcg acgggggagc
     2461 ggcggtacgt ctaccacggt aacgacggca ccacgatgcc ttggaacgac acggcgcagc
     2521 tcgactacct gcgagccgac gtgcgcgagg cggtcatgcg gacggtggaa gaggtcgcgc
     2581 gccgcttccc catcatccgg ttcgacgccg ccatgacgct ggccaagaag cacgtccagc
     2641 gcctctggca cccgctgccc ggcgagggcg gggccgtgcc atcgcgagcg cgcttcgcca
     2701 tgggccagga ggagttcgag cggcgcatgc cgcgcgagtt ctggcgcgaa ctcgtcgacc
     2761 gcatggccga acgcgctccc gacacgctgc tgctggcgga ggccttctgg ctgctcgagg
     2821 gttacttcgt gcgcgagctc ggcatgcacc gcgtctacaa cagcgctttc atgcacatgc
     2881 tcatgcgcga ggagaacggc gcgtaccggc gcctgctcaa gtcggtgctc gcgttcgacc
     2941 cgcgggtact gcagcgctac gtcaacttca tgagcaaccc cgacgaggag acggccaggg
     3001 agcagttcgg gacgggcgac aagtacttcg gcgtggcgac gctgttggcc accctgcccg
     3061 gactgccgat gttcggccac ggtcaggtgg agggcttgca agagaagtac ggcatggagt
     3121 atctggcgcc gaagctggac gagtggccgg agccttggct ggtggagcgc cacaggcgcg
     3181 aggtggcgcc gctcctgcga gcgcggggga ccttcgccgg cgcggacgcc ttcaggctgt
     3241 tcgacctgat cggggagcac ggcgtggtag aggacgcgtt cgtgttcgtg aaccgcggcc
     3301 agggcggcgc tcacggcgcg gtactagtcg cgtacaacaa ctcgccgcgc gcggtcgagg
     3361 gccgggtgcg cgtcagcgcg ccatacgcgg acgtcatgcg gggaggcggc ctccgttccg
     3421 ataccgtggt cgccgcgctc gctctgcgcg agggtggggc cggtcaggtc gcgttcacga
     3481 ccttcgccac cggcctccgc acgcgcacgg tccggatcgc cgaactcaag cgcgaggggc
     3541 tatggcttca gctcggaccg tacgaggcgc gtgtcgagct ggtcagccag accgacgacg
     3601 ggccaggcga gccggcggag gtaccggcgc tccccgccga gcgcgcccgg cggctcggga
     3661 cggcgttcgg gctcgacccg cggctagccg gcccgagggg cgtggccggg aggcgggcca
     3721 ccaagcgcag gcgctcctcc ggcgcgaggc cgccacgccg gcgccgatga cgccggcaag
     3781 aagacttgac ggttggcagc cgctcgtcag gcgaccttcg aggccggacg gggctgggct
     3841 cgggccggac ttccgccggt caccgacgcg ccgacgcgtc ggtcagcatc ccgatgcggc
     3901 gcgactgcag atgggtcaat gcgagcgcca gagcgtcggt gacatgatcg gggcgcttct
     3961 gcgccgtcag cttcagg
//
LOCUS       DAOQYE010000065         3969 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_23303_length_3969_cov_3.064129, whole genome shotgun sequence.
ACCESSION   DAOQYE010000065 DAOQYE010000000
VERSION     DAOQYE010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 3969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3969
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23303_length_3969_cov_3.064129"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="PKN52_01945"
     CDS             <1..330
                     /locus_tag="PKN52_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98731.1"
                     /translation="HQVWLDASPLASPDEAVEALHDVNLIANVNLIPSLRRKGLRLGT
                     PEATRLGMREPEMAQLAALLHATLTDTEPREQVRRRVMDIARRFRTVRYCRPAPAAQG
                     AADADAA"
     gene            327..2222
                     /locus_tag="PKN52_01950"
     CDS             327..2222
                     /locus_tag="PKN52_01950"
                     /inference="COORDINATES: protein motif:HMM:NF012550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HNQ98732.1"
                     /translation="MNPAEPHTLIERRLGAPAGREVDLAVDLIVTDDWTTPSLLPTLD
                     ALGTSRSAAPLVLVRDHTGPVESYSGGERERVLALRAIEEEFVARYGAERLVGQGIQH
                     HALPASGRLRPGMIVLGNDSHTPTLGAHGVTAFAAQPTTLAVAIHTGRLVVRVPETVR
                     VEVVGRLEPGVSARDAALTLLGLLRGGGSVPRLATGKALEFGGPGLAHLTAAERAVLA
                     NVAPEAVAVTAAFPLQEAAPTPPATGPIVTLDLGSVIPSVARSGQPSDVAPLAHLDPI
                     RVDRVFVGTCAGGTYEEVTAFATALGEKAQVPTVVSPASSGVLERLNAEGFARRLEAA
                     GVTLLPPGCGPCFGFGMRRLEDGEVAVVTGNRNSAGRMGSPHAQIILAAGRTAGLAAR
                     TGWLNPAANASSVHADAVDRRDRPRSPHAHRPTVVWPTTGNVVRLRGLVSTDDLTPSS
                     VPGVGTSSDPDPGVARRLLLHHVDPTAAERSLEGTIFVGDENFGMGSNRASSVRALLR
                     AGVLAVIAPSIAPLYQLGARDEGLPTITLTDEAFYAALGPEATLEVDFRAGTVTLDPT
                     TSSGRAPDRPPARFRFAPIGPYEAAVIAAGGAVPYLRSSGMEAGNAQAPQARRPERLA
                     PRADRAP"
     gene            2296..3810
                     /locus_tag="PKN52_01955"
     CDS             2296..3810
                     /locus_tag="PKN52_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HNQ98733.1"
                     /translation="MFAAVTSATIQGVDALEVRVEAFVSGGLPSFTIVGLPGAAVQES
                     RERVRANLKLLGYPLPPSRILVNLAPADVRKEGPALDLPIALALLACDRRIPRGALDG
                     LIAFGELALDGSVRPGRGGVAVASMAALGAHRGGGGHVLASPENAAEAALVPGVTVFA
                     AANLAAAVAHLTGRTPLVPLAPAPTTPEALPAEPLPDLATVRGQALARRALEVAAAGR
                     HNLLLTGPPGAGKSMLARCLPGILPPLTDAEAIEVTRVHSSAGHLVSGLVRAPPFRQP
                     HHSGSEAGIVGGGAVARPGEASLAHLGVLFLDELPEFSRSVLEALRQPLEEGVITISR
                     ASGSVRLPASFQLVAARNPCPCGYHGAVDGARTCTCHPAAVERYQRRLSGPLLDRMDL
                     LIGVARLTDAELVGVSQGEASDAVAARVVAARAKAVERQGFANARLSGRQLTTHTASG
                     PAGQAVLAKLVRSLRPSARGYDRLLRVARTVADLAGEDDVLPEHLLEAAAYRGA"
BASE COUNT          506 a         1523 c         1398 g          542 t
ORIGIN      
        1 caccaggtat ggctcgacgc cagccccctc gccagccctg acgaggccgt cgaagcgctg
       61 cacgacgtca acctgatcgc caacgtcaac ctcatcccgt cgctgcgccg caaggggttg
      121 cgcctcggca cgcccgaggc gacgcgcctc gggatgcgcg aaccggagat ggcgcagctc
      181 gccgccctcc tgcatgcgac gctcacggac accgagccgc gcgagcaggt ccgcaggcgc
      241 gtcatggaca tcgccaggcg ctttcggacc gtccggtact gccgccccgc gccagccgcc
      301 cagggggctg cggacgccga cgccgcgtga acccggcaga accccacacg ctcatcgaac
      361 gccggttggg cgccccggcc ggtcgggaag tcgatctcgc ggttgacctg atcgtcaccg
      421 acgactggac caccccgtct ctcctcccga ccctcgacgc gctcggcacc tcgcggtccg
      481 ccgcgccgct cgtgctcgtg cgcgaccata cgggcccggt cgagagctac tcgggtggcg
      541 agcgcgagcg ggtactcgcg ctcagggcga tcgaggagga gttcgtggcc cggtacggcg
      601 ccgagcgcct cgtcggtcag gggatccagc atcatgccct ccccgccagc ggacggttgc
      661 ggcctggcat gatcgtgctc ggcaacgact cgcacacgcc cacgttgggg gcgcatggcg
      721 tgacggcgtt cgcggcgcag ccgaccacgc tcgcggtcgc catccacacg gggcgcctcg
      781 tggtccgcgt gccggagacc gtgcgggtcg aggtggtcgg ccggctcgaa ccgggtgtga
      841 gcgcgcgcga cgccgcgctc acgctcctag gtctcctccg gggcggcggg tcggtgcctc
      901 gcctcgccac cggcaaggcg ctcgagttcg gcggtcctgg cctggcccac ctcaccgccg
      961 cggaacgcgc cgtactcgcc aacgtcgccc ccgaggccgt ggccgtcacg gcggccttcc
     1021 cgctccaaga ggcggccccg accccgcccg ccacggggcc gatcgtcacg ctcgacctcg
     1081 ggtccgtgat cccctccgtg gcccgcagcg ggcaaccgtc cgacgtggcg ccgctcgcgc
     1141 acctcgaccc aatccgcgtc gaccgcgtgt tcgtgggtac ttgcgccggt ggcacttacg
     1201 aggaggtaac ggcgttcgcg accgcgctgg gcgagaaggc ccaagtccct accgtcgtct
     1261 cgccggcctc cagcggagtg ctcgagcgcc tgaacgccga agggttcgcg cggcgactcg
     1321 aggccgccgg cgtgactctc ctaccgcccg ggtgcggccc gtgcttcggc ttcgggatga
     1381 ggcgcctcga ggacggcgag gtcgccgtcg tgacggggaa ccgcaacagc gccggccgca
     1441 tgggctcgcc ccacgctcag atcatcctcg ccgccggccg caccgctgga ctggccgcgc
     1501 gcaccggctg gctcaacccc gccgccaacg caagtagcgt ccacgcagac gccgtagacc
     1561 gacgagaccg gcctcggtcg ccacacgctc accgcccaac ggtggtctgg ccgactaccg
     1621 gcaacgtggt caggctgcgc ggcctcgtct ccacggatga cctgacgcct tccagcgtgc
     1681 caggcgtcgg tacgtcgagc gaccccgacc ccggcgtggc gcggcggctc ctcctccacc
     1741 acgtcgatcc gacggctgcc gagcgctcgt tggagggcac catcttcgta ggagacgaga
     1801 acttcggcat gggaagcaac cgggcgtcct ccgtgagggc gctgctgaga gcaggcgtcc
     1861 tggccgtcat cgccccgagc atcgcgccgc tctaccagtt aggggcgcgc gacgaggggc
     1921 tgccgacgat cacgctcacc gacgaggcgt tctacgcggc cctcggcccg gaagcgacgc
     1981 tggaggtgga cttccgtgcc ggcacggtga cgctcgatcc cacgacgtct tccggccgcg
     2041 cgcctgaccg acctcccgcc cgcttccgct tcgctcccat cggcccttat gaagcggccg
     2101 tcatcgcggc cggcggcgcc gtcccgtacc ttcgttcctc cgggatggaa gcgggcaacg
     2161 ctcaggctcc tcaggcgagg cgcccggagc ggctggcgcc acgcgccgac cgggccccgt
     2221 aaagctgcct agaaccggtc ggcaaccgcc gtggagctgg acgcctccag ccacgtcaga
     2281 cgctagcgtc gccgtgtgtt cgcagccgtc acctccgcca ccatccaggg ggtggacgcg
     2341 ctggaggtgc gcgtcgaggc gttcgttagc ggcggcctgc cgagcttcac catcgtcggg
     2401 ctgccaggag cggcggtgca ggagtcgcgc gagcgcgtcc gggccaacct caagctgctc
     2461 ggctacccgc tgccgcccag ccggatcctc gtcaacctgg ccccggccga cgtacgcaag
     2521 gagggaccgg cgctcgacct gcccatcgcg ctcgcgctgc tggcgtgcga ccggcggata
     2581 ccccggggcg cccttgacgg gctcatcgcc ttcggggaac tcgcgctcga cggcagcgtc
     2641 aggcctgggc gcggcggcgt ggccgtcgcg agcatggccg ccctcggcgc gcaccggggc
     2701 ggcggcgggc acgtgctcgc gtccccggag aacgcggccg aggcggccct cgtgcccggc
     2761 gtcaccgtgt tcgccgcggc caacctggcg gccgcggtgg cccacctcac cggccgaacg
     2821 ccgctggtgc cgttggcgcc tgctcccacc acgccggaag cgctccccgc ggagccgctg
     2881 ccggacctcg ccacggtgcg tgggcaggcc ctggcacgcc gggcgctcga ggtggccgcg
     2941 gcggggcggc acaacctcct cctcacgggt ccacccggcg cgggcaagag catgctggcg
     3001 cgctgtctgc ccggcatcct gccgccgttg accgacgcgg aggccatcga ggtcacgcgc
     3061 gtccactcca gtgcgggcca cctcgtgagc ggcctcgtcc gcgccccgcc cttccggcag
     3121 ccgcaccact ccggctcgga ggccggcatc gtcggcggcg gcgcggtggc gcgtcctggc
     3181 gaggcgagcc tcgcgcacct aggcgtcttg ttccttgacg agctcccgga gttctcgcgc
     3241 tcggtgttgg aggcgttgcg gcagccgctc gaggagggcg tcatcaccat ctcgcgcgcg
     3301 agcggcagcg tccgcttgcc tgcctcgttc cagctcgttg ccgcgcgcaa cccgtgtccg
     3361 tgcggttacc acggcgccgt cgacggcgcc cgcacgtgca cgtgtcatcc ggccgccgtg
     3421 gagcgctacc agcggcgcct gtccggcccg ctcctcgacc gcatggacct gctgatcggc
     3481 gtcgcccgcc tcacggacgc ggagttggtg ggcgtgtcgc aaggcgaggc tagcgacgcc
     3541 gtggccgcca gggtggtggc ggcccgcgcc aaagccgttg agcgccaggg cttcgccaac
     3601 gcccggctct ccggccggca gctcacgacg catacggcaa gcgggccggc cgggcaggcg
     3661 gtcctggcca agctcgtgcg gagcctgcga ccctcggcgc gcggctacga tcgactcttg
     3721 cgcgtggccc gcacggtcgc cgacctggcc ggagaagacg acgtgctccc ggagcacctg
     3781 ctggaagcgg ccgcgtaccg cggcgcgtag ccgtccgtgc gggcgcgtcc gtgcgggcgc
     3841 gacggtttgt gggtgcgccc gcgtgggcgc gtaggtgttg gcgcgacgag gcggggcatg
     3901 acccgagcgg gcacccgcgc atgcaaatgc acccccgcag ataaatgagg atccgatggc
     3961 atttcgatg
//
LOCUS       DAOQYE010000066         3954 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_23433_length_3954_cov_3.031803, whole genome shotgun sequence.
ACCESSION   DAOQYE010000066 DAOQYE010000000
VERSION     DAOQYE010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 3954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3954
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23433_length_3954_cov_3.031803"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2000
                     /gene="leuS"
                     /locus_tag="PKN52_01960"
     CDS             <1..2000
                     /gene="leuS"
                     /locus_tag="PKN52_01960"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HNQ98734.1"
                     /translation="NWDPVDQTVLANEQVIDGRGDRSGALIERRLMPQWHFRITRYAD
                     ELLDFSSLDWPERVVAMQTNWIGRSEGAEVTFKTEAGDDIVVFTTRPDTLWGATFMVL
                     APEHPLVEKLATPERRDAVRAYVERASRRTDLDRMADTKEKSGEFLGAYAVNPVNGAR
                     IPIWIADYVLMGYGTGAIMAVPYGDQRDFEFARDFGLEIVPVVRPAGVEGALSAADLT
                     EAWAGPGVMVNSGPLDGLAHNGEKGRASPAIAAAIEHLERNGLGRAQTTYRLRDWLIS
                     RQRYWGTPIPIVYCDACGMVPEKYENLPVVLPEDVAFMPTGESPLKTHAAFLETTCPR
                     CGGKARRETDTMDTFMDSSWYWFRYLSPHDDTRPFDPELAARWTPVDTYTGGIEHAIL
                     HLLYARFFTKVLRDLGLTTCDEPFKRLRNQGMILGADNEKMSKSRGNVVNPDDLVARY
                     GADTVRAYLMFLGPWDQGAPWNYSGIEGQSRFLGRVWNLVVDEAKVDAPGAVSETELN
                     RAINHAVKEVTEDFEAFRFNTAIAELMTLQNALQKARAAGLAGSATWRHGVEALLLLL
                     APIAPHITEELWHRLGKTGSVHLQPWPTHDPGALVANTVQMAVQVNGKLRGQVEVPVE
                     ADDTAIVAVAQADGNVAKHLEGRQVVRSIVVRGKLVNFVVK"
     gene            complement(2017..2799)
                     /gene="mreC"
                     /locus_tag="PKN52_01965"
     CDS             complement(2017..2799)
                     /gene="mreC"
                     /locus_tag="PKN52_01965"
                     /inference="COORDINATES: protein motif:HMM:NF016011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HNQ98735.1"
                     /translation="MSSLLRAWYLFIALGLFTVLFTAFVGTVPADVTATIALPHELLH
                     RAGTNVRLALESAVDRRDLRALVAELGERLAAEQEATRGLELELDRYREALTVAQTQS
                     PGVLAGAPVIGSDAGAELARLRVGLGENAGVRRNLPVTVPEGLVGIVTDVARDSSIVR
                     TVVDPQSRVGVTVRDKGGQGVAVGEVGGLVRVSRFMLDQPIVVGDVVETSSFGGLFPA
                     GIKVGVVIEVAPVDPNDLRRTFIVRPSVEIGTLQQVVLLAPQ"
     gene            complement(2792..3478)
                     /locus_tag="PKN52_01970"
     CDS             complement(2792..3478)
                     /locus_tag="PKN52_01970"
                     /inference="COORDINATES: protein motif:HMM:NF014591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HNQ98736.1"
                     /translation="MNADNLDRSAGAVAAARRADGDERLPGGTDGGAVGSPDRLPRIV
                     LASGSPRRSQLLGSIGLTFSVVVPEVDETPAHGEAPQDLVERLAASKALAVAATHRDA
                     LVIAADTTVAVDGAILNKPTDEAENREFVRRLAGREHEVFTGHALTYAGELETVVVRS
                     AVVFRPLSEAEIAGYCATGEGRDKAGGYAIQGRGAALIEGIRGCYPNVMGLSLVNVVL
                     TARRLGVDLV"
     gene            3648..>3954
                     /locus_tag="PKN52_01975"
     CDS             3648..>3954
                     /locus_tag="PKN52_01975"
                     /inference="COORDINATES: protein motif:HMM:NF017806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase A"
                     /protein_id="HNQ98737.1"
                     /translation="MSEAITGLEAMKRAAALRALALVESGMLLGLGTGSTARWLVEEL
                     GAALARGELMGVRGVATSKQTETQAAGLGIPLVELPPDGVDLAIDGMDELTPRLDAIK
                     "
BASE COUNT          629 a         1435 c         1340 g          550 t
ORIGIN      
        1 tcaactggga cccggtcgac cagacggtgc tcgccaacga gcaggtgatc gacgggcgcg
       61 gcgaccgctc gggcgccctc atcgagcggc gcctcatgcc gcagtggcac ttccgcatca
      121 cgcgctacgc cgatgagctc ctcgacttct cgtcgctcga ctggccggag cgcgtcgtcg
      181 ccatgcagac gaactggatc gggaggtcgg agggcgccga ggtgacgttc aagacggagg
      241 ccggcgacga catcgtggtg ttcacgactc gcccagacac gctctgggga gctaccttca
      301 tggtcctcgc ccccgagcac ccgctggtgg agaagctcgc tactcccgag cgacgcgatg
      361 ccgtgcgcgc ctacgtcgag cgcgcctccc gccgcaccga cctcgaccgc atggccgaca
      421 cgaaggagaa gtcgggggag ttcctcggcg cctacgccgt caaccccgtg aacggggctc
      481 gcatccccat ctggatagcc gactacgtgc tcatgggcta cggcacgggc gccatcatgg
      541 ccgtgccgta cggcgatcag cgcgacttcg agttcgcgcg cgacttcggg ctcgagatcg
      601 tgccggtggt gcgcccggcc ggggtcgagg gcgcgctgag cgcggcggac ctgaccgagg
      661 cgtgggctgg gcccggtgtg atggtcaact ccggcccgct cgacgggctc gcgcataacg
      721 gggagaaggg ccgcgccagc cccgccatcg cggcagccat cgagcacctc gagcgcaacg
      781 gcctcggccg cgcccagacg acgtaccgac tgcgcgactg gctcatcagc cgccagcgct
      841 actggggcac gcccatcccc atcgtgtact gcgacgcctg cggcatggtc ccggagaagt
      901 acgagaacct gcccgttgtc ctgccggagg acgtggcgtt catgccgacg ggcgagtcgc
      961 cgctcaagac ccacgccgcg ttcctcgaga cgacctgccc ccgttgcggc ggcaaggcgc
     1021 gccgcgagac cgacaccatg gacacgttca tggactcgtc ctggtactgg ttcaggtacc
     1081 tgagcccgca cgacgacacg cgcccgttcg accctgagct tgccgcgcgc tggacgccgg
     1141 tcgacacgta cacgggcggc atcgagcacg cgatcctcca cctcctgtac gcgcgcttct
     1201 tcacgaaggt cctgcgcgac cttggcctga ccacgtgcga tgagcccttc aagcgcctgc
     1261 gcaaccaggg catgatcctc ggggcggaca acgagaagat gtcgaagtcg cgcggcaacg
     1321 tcgtcaaccc cgacgacctg gtcgcccgtt acggtgccga cacggtgcgc gcctacctca
     1381 tgttcctcgg tccgtgggac cagggggcgc catggaacta cagcggcatc gaggggcagt
     1441 cacgcttcct cgggcgcgtc tggaacctcg tggtcgacga ggccaaggtc gacgcccccg
     1501 gcgccgtctc cgagaccgag ctgaaccggg ccatcaacca cgccgtcaag gaggtcacgg
     1561 aggacttcga ggccttccgc ttcaacacgg cgatcgccga gctcatgacc ctgcagaacg
     1621 cgctgcagaa agcacgcgcg gccggcttgg ccgggagcgc cacgtggcgg catggcgtcg
     1681 aggccctctt gctcctcctg gcgccgatag cgccgcacat cacggaggag ctctggcacc
     1741 gcctcggcaa gacgggctcc gtgcacctgc aaccgtggcc cacccacgac cccggcgctc
     1801 tggtggcgaa caccgtgcag atggccgtgc aggtcaacgg caagctcagg ggtcaggtcg
     1861 aggtgccggt cgaggccgac gacacggcga tcgtcgcggt cgcgcaggct gacggcaacg
     1921 tggctaagca tctcgagggc aggcaggtcg tgcgctccat cgtcgtgcgc ggcaagctcg
     1981 tgaacttcgt ggtcaagtag cgcggcggcg cccacctcac tgcggggcga gcaggacgac
     2041 ctgctgcaac gtaccgatct ccacgctcgg acgcactatg aacgtgcggc gcaggtcgtt
     2101 cgggtcgacc ggcgcgacct cgatcacgac gcccaccttg atgccggccg ggaagaggcc
     2161 gccgaagctc gacgtctcca ccacgtcgcc gaccacgatg ggctggtcga gcatgaagcg
     2221 ggatacgcga acgagcccac ccacctcgcc gacggccacg ccctgtccgc ccttgtccct
     2281 gaccgtcacc ccgacgcgcg actgcgggtc gacgacggtg cgcacgatgg acgagtcgcg
     2341 cgcgacgtcg gtgacgatcc ctaccagccc ctccggcacg gtgacgggaa ggttgcgtcg
     2401 cacgcccgcg ttctcgccga ggccgacgcg cagcctcgca agttccgcgc cggcgtcgct
     2461 cccgatcacg ggggcgcccg ccagcacccc gggagactgc gtctgcgcga ccgtcaaagc
     2521 ctcccgatag cggtctagct ccaactccaa ccccctggtc gcctcctgct cggctgcgag
     2581 ccgctcgccc aactcggcca ccagcgcgcg cagatcgcgc cggtccacgg cgctctcgag
     2641 cgccaacctc acgttcgttc ccgcgcggtg cagcaactcg tgagggaggg cgatggtcgc
     2701 cgttacgtcc gccggcacgg tgccgacgaa ggccgtgaag aggaccgtga agagcccgag
     2761 ggcgatgaac aggtaccagg cgcgcaggag gctagacaag gtcgaccccg agccgcctgg
     2821 ctgtcaagac cacgttgacc aggctgaggc ccatgacgtt cgggtagcag ccgcggatgc
     2881 cctcgatgag ggcggctccc ctgccctgga tggcgtagcc gcccgctttg tcgcgccctt
     2941 cgccggtggc gcagtagccg gcgatctcgg cttcggaaag cggtctgaac actacggcgc
     3001 tccgcaccac taccgtctcg agctcacccg cgtacgtcaa ggcgtgaccg gtgaacacct
     3061 cgtgctcacg gcccgccagt cgccgcacga actcgcggtt ctccgcctcg tcggtcggct
     3121 tgttcaggat cgcgccgtcg accgccacgg tggtgtctgc ggcgatcacg agggcgtcgc
     3181 ggtgagtagc ggccaccgcg agcgccttgc tcgcagcaag gcgctcgacg aggtcttgtg
     3241 gcgcctcgcc atgcgccggg gtctcgtcga cctccggcac gacgacgctg aacgtcaagc
     3301 cgatgctgcc gaggagttgc gaccggcgcg gcgagccgct ggccaggacg atgcgaggca
     3361 gtcggtccgg cgagccgacg gcgccgccat cggtgccgcc cggcaagcgc tcgtcgccat
     3421 ccgcccggcg agcggcagcc acggccccgg ccgacctgtc aaggttgtcc gcgttcatcc
     3481 cagacactcg gcacaggata acggctcgac tcgcaccctt ggtccgaagc cgcccggacg
     3541 cgccaacccg cgcggccacg cggacgcgcc gacccggccg cgggacgggt gtcggtgcgg
     3601 cggccgacgc ccgcgcgctg tgagtgcatg acggtatgct cctgggcatg agcgaagcga
     3661 tcaccggcct ggaggccatg aaacgcgccg cggcgctgcg ggccctcgcc ctcgtggaga
     3721 gcggcatgct cctcggactc ggcaccggca gcacggcgcg ctggctcgtc gaggagctcg
     3781 gggcggcgct cgcgcgcggt gaactgatgg gcgtgcgcgg cgtcgccacc tccaagcaga
     3841 ccgagacgca ggccgctggg ctcggcatcc cactggtgga gctgccaccc gacggcgtcg
     3901 acctcgcgat agacggcatg gacgagctca ccccccgact ggacgccatc aagg
//
LOCUS       DAOQYE010000067         3949 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_23469_length_3949_cov_2.984078, whole genome shotgun sequence.
ACCESSION   DAOQYE010000067 DAOQYE010000000
VERSION     DAOQYE010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 3949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3949
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23469_length_3949_cov_2.984078"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(146..1498)
                     /locus_tag="PKN52_01980"
     CDS             complement(146..1498)
                     /locus_tag="PKN52_01980"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNQ98738.1"
                     /translation="MSHIGRKAPHNDLPSEHDVDVYDVLVIGAGAAGLAAAQALLERR
                     SDLEVKVLEGRERLGGRMHTDRRFAGFPIELGAEFIHGERATTWRYVERLGLETVRWE
                     KLDDSWVRLADGRRLTMRQARAEDPALEVTRSWHLPPLSARKHESFDAYLRRSGFDPV
                     RLDYVRRAYANAAGESLRFLDAAAALEGIAGLGLDGTEDHRIVEGYGAIVEALALGPE
                     FYTGTVVTRIEWGPGRVVVHDNEGHVYRSRMAIVTLPVGVLQAGSVEFDPPLPDAKEQ
                     ALAGLGMGPVVKLVYRFAEPVTPEEITAVYAAGNPPMWWSPSAGHPTTEAVVWTAFVT
                     GDGAVELMRLGEDDALERGLDALRRELGRPGLRAQDAFVVDWTSDPFARGGYSHVRPG
                     HHGARELLAAPTPPLFWAGEATAPEARAATVHGAILSGERAAAEVLVAVSSMAVDLAS
                     "
     gene            complement(1514..2248)
                     /locus_tag="PKN52_01985"
     CDS             complement(1514..2248)
                     /locus_tag="PKN52_01985"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNQ98739.1"
                     /translation="MRGKEVLEFTVASTQLRHGHGVYDVVRLANGYPTDRYVHCINPE
                     AVAEQLERFPEGQFVATTYVDGMEKVIGMATTMRTARTPDEPALPWYDVIGSFGLRNH
                     DPEGEWLYGVEIAVHPDYQRRGVATALYKARLALVEQLGLRGWYAGGMLMGYDRYRET
                     FTPKEYAQRVIAGDLDDPTVTMQLHQGLEGRAIIEDYYPEWRAGNTAVLLVHEPKRAP
                     RRPGGPNAPKLRSSTRDARMLQEPAS"
     gene            2731..3303
                     /locus_tag="PKN52_01990"
     CDS             2731..3303
                     /locus_tag="PKN52_01990"
                     /inference="COORDINATES: protein motif:HMM:NF019468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HNQ98740.1"
                     /translation="MQVSREEAKLVTAGLRTPPAGLDLALEILAALVVAVALAFLIVS
                     WPGLPERVPTHFDASGTPDSFGPRASLWGLAAVMVGLYGVLYAMKYFPPRFWNVPFKV
                     TEVNAEAAGRVMARFARAMNLACTGLFAAILFGSVRVALGQTGGLPVWLMLAGAVLPL
                     GALVWALVEMSRLSSEASVGKASARHHGSR"
BASE COUNT          570 a         1310 c         1411 g          658 t
ORIGIN      
        1 ggccccctgg ccgcgcacgg ggcgcagctc ggtgaggcgc gaccagacgg gcgctaggtg
       61 cgtcccggat gggttggtgt tgacggcttc tggtgccatg tcgactcctt acttacgtac
      121 ggcctgccgg tacggctgtc caagatcaag aggctaggtc tactgccatc gatgatacgg
      181 cgacgagtac ctcggcggcc gcgcgctcac cgctgaggat ggcgccgtgc acggtggcgg
      241 cgcgcgcctc cggggcggtg gcctcgccgg cccagaagag gggtggggtc ggggccgcga
      301 ggagttcgcg ggcgccgtgg tgaccgggcc tcacgtgcga gtagccgccg cgggcgaacg
      361 ggtcggaggt ccaatccacg acgaacgcgt cctgggcccg caggccgggc ctgccgagct
      421 cgcgccgcag cgcgtcgagg ccgcgctcca gcgcgtcgtc ctcaccgagc cgcatgagtt
      481 caacggcgcc gtcgcccgtc acgaacgccg tccagacgac ggcctccgtg gtcgggtgcc
      541 cggctgaggg cgaccaccac atgggcgggt tgccggcagc gtagacggcg gtgatctcct
      601 ccggcgtgac cggctcggcg aagcggtaga cgagcttgac aacggggccc atgccgaggc
      661 cggccagcgc ctgctccttc gcgtctggta gcggcgggtc gaactcgacg ctgcccgcct
      721 gcaacacccc gaccggcagg gtgacgatgg ccatgcgcga gcggtagacg tgcccctcgt
      781 tgtcatggac gacgacgcgt ccggggcccc actcgatgcg cgtgacgacg gtgcccgtgt
      841 agaactccgg tccgagcgcg agcgcctcga cgatggcccc gtagccctcc acgatgcggt
      901 ggtcttccgt gccgtccagc cccagcccgg ctatcccttc gagggcggcg gccgcgtcga
      961 ggaagcggag gctctcgccg gcggcgttgg cgtaggcgcg ccgcacgtag tcgagccgca
     1021 cggggtcgaa gccgctgcgg cgcaggtaag cgtcgaacga ctcgtgcttg cgcgcactca
     1081 gcggcggcag gtgccacgag cgcgtgacct cgagggccgg gtcctccgcg cgcgcctggc
     1141 gcatggtgag gcgccgcccg tcggcgaggc gcacccagga gtcgtccagc ttctcccagc
     1201 ggacggtctc caggccgagg cgctcaacgt agcgccacgt ggtcgcacgc tcaccgtgga
     1261 tgaactcggc gccgagctcg atggggaagc cggcgaagcg cctgtcagtg tgcatgcggc
     1321 cgcccaggcg ctcgcgcccc tcgaggacct tgacttcgag gtcggagcgg cgctcgagca
     1381 aggcctgggc ggccgccagg ccggccgcgc cggcaccgat gacgagcacg tcgtacacgt
     1441 cgacgtcgtg ctcgctcggc agatcgttgt ggggcgcctt gcgcccgatg tggctcatcg
     1501 gccgtcctta aagtcagctg gcgggttcct gaagcatccg cgcgtcgcgg gtcgaagagc
     1561 gcagcttggg ggcgttcggg ccccccgggc gcctgggcgc gcgcttcggc tcgtgcacga
     1621 ggaggacggc cgtgttgccg gcgcgccact cggggtagta gtcctcgatg atggcgcggc
     1681 cttccaaccc ctggtggagc tgcatggtga cggtcgggtc gtcgagatcg ccggcgatca
     1741 cgcgctgagc gtactccttg ggggtgaacg tctcgcggta gcggtcgtag cccatgagca
     1801 tgccgcccgc gtaccagccg cgcaggccga gctgctcgac gagggcgagc ctggccttgt
     1861 agagggcggt ggccacgccc ctgcgctggt agtcgggatg gacggcgatc tcgacgccgt
     1921 acagccactc cccttccggg tcgtggttgc gaaggccgaa ggaaccgatg acgtcgtacc
     1981 aggggagcgc cggctcgtcg ggcgtgcgag ccgtgcgcat ggtggtcgcc atgccgatga
     2041 ccttctccat gccgtccacg taggtggtag ccacgaactg cccttccggg aaccgttcga
     2101 gctgctcggc gaccgcctcc gggttgatgc agtggacgta gcggtccgtt gggtagccgt
     2161 tggcgagcct gacgacgtcg tagacgccgt gaccatgccg caactgtgta ctggctacgg
     2221 tgaactctag tacttctttg cctctcataa cgtcgcttca acaacgcctg ttgaggtatg
     2281 tgagcccgta aaagggggaa actgaaacgg ttagggaagg gttactacgg tccctgaccg
     2341 gaagagcttc tccacaacct ttacgcgaga gcgttcgcct cccgagacca tcagcagatg
     2401 gctcattcgg tctcactcac actttagggt tgtcttgaag tttctcacgc tccttcttgc
     2461 ccgtttggta ggagtgtagc acgcgttcag gaagccccga cgtgacgcaa cgcacagata
     2521 tcgtgcagga cgcggtttcg ttgggcttga caaaggcgcg ctcaggacgt ataggcgctg
     2581 ggttcgcacg gcgggacgcg cggagcgctc cgcgaggggc ttacgcgtgc gccgccaccc
     2641 gtggtaccga tacggtggtc gaccttctcg cggttacatc ggtggcgcgc gaccgaccga
     2701 tcggccgccc gatgtcggat agtctggggc atgcaggtct cccgcgagga agcgaagctc
     2761 gtcacagccg gactccggac tcccccggca ggcctcgacc tcgcgctcga gatcctcgcc
     2821 gcgctggtcg tcgcagtggc cctggcgttc ctcatcgtct cgtggccggg gttgccggag
     2881 cgcgtgccga cgcacttcga cgcctccgga acgccggact ccttcggccc tcgcgcgtcc
     2941 ctgtgggggc ttgcggcggt gatggtgggt ctgtatggcg tcctctacgc catgaagtac
     3001 ttcccgccgc ggttctggaa cgtgcccttc aaggtcaccg aggtcaacgc cgaagccgcc
     3061 ggtcgggtga tggcgcgctt cgctagggcc atgaacctcg cttgcaccgg cctgttcgcc
     3121 gccatcctct tcggcagcgt gcgtgtggcg ctcgggcaga caggcgggct accggtatgg
     3181 ctcatgctgg ccggcgccgt tctgccgctg ggcgctctcg tctgggcgct ggtggagatg
     3241 tcgcgtctga gctccgaagc gtccgttggc aaggcgagcg ctcggcatca cgggtcgcgg
     3301 tagccgatca cggccagggc gcatacctcg cccgcgtcct cgacctcatc cgcctcccta
     3361 ccaccgcgct agccacgcgg ccgcccgcct cgtggacgaa tgtaggcgct cgggttttca
     3421 aacgatagga ggggcgttga agttcgggct cacgcccttc ggcaggcccc gctccgcgtc
     3481 accgccacct tgtgagccgg cgccgaagca tgtgcaaaag gttaagaagc gagaaaatgg
     3541 gatagtagaa agcgagaaag ggcgtcgaca cgggcccgac ctcctaccgc ggtcgcgacg
     3601 gcgtctgggt ggtgccagcg gcgctgttgg gcgcctgagg cgggggaacg ccgcgggttc
     3661 gaaccaaggc cactcaagca gaccgacccg accggttcgg caccaaactg cgcggcgctc
     3721 cggggcccgg caggaccatc tccaggagcc ggctcctgcg gtatgctcgc ctccgatgct
     3781 cgcagcgagg caccgacgag cgcgacgcgc caagcgccgc cagggtggtg gactagcatg
     3841 acgcgcggca aggtcagggg cgacagggcc agagccgttg gcatggcgtt gccggccacg
     3901 ttctggctcg tggtcttctt cctgctgccg ctcgtgttcg tggcgttct
//
LOCUS       DAOQYE010000068         3944 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_23520_length_3944_cov_3.643353, whole genome shotgun sequence.
ACCESSION   DAOQYE010000068 DAOQYE010000000
VERSION     DAOQYE010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 3944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3944
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23520_length_3944_cov_3.643353"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="PKN52_01995"
     CDS             complement(<1..445)
                     /locus_tag="PKN52_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNQ98741.1"
                     /translation="MRSSQGTPRRRKLSLTTREALWAYAFLLVPLAFFLFFRLWPAVQ
                     SLKLSFFTWHVDPEQRTFIGLQYYREMYANFAAKGDLYKALRNMLYYFLLVVFGQVGI
                     GLFSAVLLNSINWFKGLFRAIYFAPYITPAAAVAWVWGWMYSVNFG"
     gene            complement(649..1896)
                     /locus_tag="PKN52_02000"
     CDS             complement(649..1896)
                     /locus_tag="PKN52_02000"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNQ98742.1"
                     /translation="MKRFLILLAVMVGVSIGAAQTTITYWQYDYATRVDAMNQLIAQF
                     EAANPDIKVVQETFPYDGYPAQVAASIPAGQGPDVAQLFYGWLPTWARGGYVVPLPDQ
                     YFDAAELDANFAPLVQAAKVDGTWYGLPTAVRSLALFYNADMLAAAGYDAPPATWDEF
                     VEIAQALTVKEGNRLVQVGYGFAPNGQDHHLVREVLVRQFGGAPYSDDYSTVTYADQA
                     GIDALTFYTDWVTKYGFAVPEFVPGNNGYRDGFRQLENIAMIVDGSFAIGDVQKVTFN
                     WGVTELPVRAENGVQSNFGSFFMNALTPNATKDPAKLEAASRFLKFVTSDDAMKLWLS
                     VVGELPASRSLIADPALAADPVYGPFVRALDYAHATVFVDETGQRDTMVDAINEVVLQ
                     GTSPADALKAAAAEEQLLLDANR"
     gene            complement(1893..3344)
                     /locus_tag="PKN52_02005"
     CDS             complement(1893..3344)
                     /locus_tag="PKN52_02005"
                     /inference="COORDINATES: protein motif:HMM:NF012432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HNQ98743.1"
                     /translation="MSWHPAYRKQVLEPDFRHAAAHLSHHFIDAMTAHLDTVMRLPAY
                     SAPEQQAAGARVRAALVAQHELPAPEQGDDVPDYYFALQRRLEGELGEAVGLIRVGLS
                     RSDLDMTVYKMAGREELLDVGERLVALRGLILAKAAKHVETVLIAQTHHQPGQPTTVA
                     HYLGAVEDQLARDSRRAAEAYARMNTCPLGAAALAGSSHPLERGFTAASLGFGAPVAN
                     TYDAVASADWEFDIVSVAQSISLGLGRFVNDLLSWAASGWYRLEDSLVQGSSIMPQKR
                     NPVSLEHARTRLSRVLGSAGMVTYSHHNIPFTDLNDFGPDIQGALGTQHRQLVGALTL
                     LAACLEQGDFDERRLAEAALRTDTTATELADVLARDHGLTFPHAHQVVATLVRRFSTE
                     GRLLTSAAPADVEAAGGPPVSAEALAAAVDPSGFVRRRAGYGMPAPEVMTNALARATE
                     RLEADRLALRERSEALALATARLRGTGKELVEA"
     gene            complement(3341..>3944)
                     /locus_tag="PKN52_02010"
     CDS             complement(3341..>3944)
                     /locus_tag="PKN52_02010"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNQ98744.1"
                     /translation="ADVELRRAVDELGLRGYKLLGPRTDHPFEAPELRGVWEFLAERR
                     LPALIHFGFLGRGGGIVQHPRMSPLSLFEVARAFPEIPFLIPHFGAGYFDDLLALCWS
                     LPNIYIDTSGSNQWMRWMPYPLTLEDLYRKALETVGAERIVFGSDSSLFPRGFSERYL
                     LDQVRACYTLGVSEAEVELIFGGNAARLLHLPEQQTGAAE"
BASE COUNT          630 a         1196 c         1499 g          619 t
ORIGIN      
        1 tgccgaagtt gacggagtac atccagcccc acacccatgc gacggccgcg gccggcgtga
       61 tgtacggggc gaagtagatg gcgcggaaaa gacccttgaa ccagttgatg gagttgagaa
      121 gtacggcgct gaagagaccg atgccgacct gcccgaagac cacgaggagg aagtagtaga
      181 gcatgttcct cagagccttg tagaggtcgc ccttggcggc gaagttggcg tacatctccc
      241 ggtagtactg cagtccgatg aaggtgcgct gctccgggtc gacgtgccag gtgaagaagg
      301 acagcttcaa agattggacg gccggccaca acctgaagaa caggaagaag gccaggggga
      361 cgagcaggaa cgcgtacgcc caaagagcct ctcgcgtggt gagcgagagc ttccggcgac
      421 gcggcgtccc ttgtgaactc ctcatggctc ctccttaagc cggttagagc gctagccttg
      481 caggcggcgt tgtcgccggc gctggtctag cagcgtgcat gaagatagcg attggcatga
      541 gtgtacaggt cgggcgcgaa cgggccgacc cgcggcgagc ttcgctgagt gcttcggtgc
      601 gtgggtgggt tcggtcgacc atgtaggtcg accgaaccca tcacggtctt agcggttggc
      661 gtcgagcagg agctgctctt cggcggcggc ggccttgagg gcgtcggccg gagaggtgcc
      721 ctggaggacg acctcgttga tggcgtcgac catggtgtcg cgctggccgg tctcgtcgac
      781 gaacacggtg gcgtgcgcgt aatcgagggc gcgcacgaac ggtccgtaga cggggtcggc
      841 ggcgagggcc gggtcggcga tgaggctacg gctggcgggc agctcgccaa cgacgctgag
      901 ccacagcttc atggcgtcgt cgctcgtgac gaacttcagg aacctggagg cggcttcgag
      961 cttggcgggg tccttggtcg cgttcggcgt gagcgcgttc atgaagaacg agccgaagtt
     1021 ggactggacg ccgttctcgg cgcgcacggg cagctcggtg acgccccagt tgaacgtgac
     1081 cttctggacg tcgccgatgg cgaacgagcc gtcgacgatc atggcgatgt tctcgagctg
     1141 gcggaagccg tcgcggtagc cgttgttgcc ggggacgaac tctggcacgg cgaagccgta
     1201 cttcgtgacc cagtcggtgt agaaggtgag ggcgtcgatg ccggcctggt cggcgtacgt
     1261 gacggtgctg tagtcgtcgg agtacggcgc gccgccgaac tggcgcacga ggacctcgcg
     1321 cacgaggtgg tggtcctggc cgttcggggc gaagccgtag ccgacctgga cgaggcggtt
     1381 gccttccttg accgtgaggg cctgggcgat ctcgacgaac tcatcccacg tggcgggcgg
     1441 agcgtcgtag ccggcggcgg cgagcatgtc ggcgttgtag aagagcgcga ggctgcggac
     1501 cgcggtgggc aggccgtacc acgtgccatc gaccttcgcg gcctggacga gcggcgcgaa
     1561 gttcgcgtcg agctcggcgg cgtcgaagta ctggtcgggg agcggaacga cgtaaccgcc
     1621 gcgcgcccac gtggggagcc agccgtagaa gagctgggcg acgtcagggc cctgaccggc
     1681 cgggatggaa gccgcgacct gcgccgggta gccgtcgtac gggaaggtct cctgcacgac
     1741 cttgatgtcg gggttggcgg cttcgaactg ggcgatgagt tggttcatcg catcgacgcg
     1801 ggtggcgtag tcgtactgcc agtaggtgat ggtggtctgg gcagcaccga tgctgacccc
     1861 caccatcacg gccaatagga ttaggaaacg cttcatgctt cgacaagctc ctttccggtt
     1921 ccgcgcagtc gcgctgtggc gagggcgagc gcctcggacc gttcccgcag ggccagacgg
     1981 tcggcttcca gccgttccgt cgcccgagcg agcgcattcg tcatgacctc aggtgccggc
     2041 atgccgtagc cggcgcggcg ccgcacgaac ccgctcgggt ccactgcggc ggcgagtgcc
     2101 tcggcggaga ccggcggacc gccggctgcc tccacgtcgg cgggggcggc cgaggtcaag
     2161 agccgccctt ccgtgctgaa acgcctcacc aaggtggcga ccacctgatg ggcgtggggg
     2221 aaggtgagtc catggtcgcg cgcgagcacg tcggcgagct ccgtcgcggt cgtgtccgtg
     2281 cgcagcgcgg cctcggcgag cctgcgctcg tcgaagtcgc cttgctcgag gcacgccgcg
     2341 agcagggtga gggcgccgac caactggcgg tgctgcgtgc cgagcgcgcc ctggatgtcc
     2401 ggaccgaagt cgttgaggtc cgtgaagggg atgttgtggt gggagtacgt gaccatgccg
     2461 gccgagccca ggacccgcga caagcgggtg cgggcatgct cgagggagac cgggttacgt
     2521 ttctgaggca tgatcgacga cccttggacc agactatctt ccaggcggta ccagccggat
     2581 gctgcccagg acaagagatc gttaacgaac cgacccaacc cgagcgagat cgactgcgcc
     2641 acgctcacga tgtcgaactc ccagtcggcg gaggcgacgg cgtcgtacgt gttggccacc
     2701 ggagcgccga agccgaggct ggccgcggtg aagccgcgct ctagcggatg gctgctaccg
     2761 gcgagcgcgg cggccccaag tgggcacgtg ttcatccggg cgtaggcctc ggccgcgcgc
     2821 cgcgagtcgc gggccagctg gtcctcgacg gcgccgaggt agtgggccac ggtggtcggt
     2881 tggccgggct ggtggtgggt ctgggcgatg aggacggtct cgacgtgctt ggcggccttc
     2941 gcgaggatca gaccccgtag ggcgacgagt cgctcgccga cgtcgaggag ctcctcccgg
     3001 ccggccatct tgtagaccgt catgtcgagg tcgctgcggc tcaggccgac gcggatgagg
     3061 ccgacagcct cgcccaactc gccctccagg cggcgctgga gcgcgaagta gtagtcgggc
     3121 acgtcgtcgc cctgctccgg ggccggcagc tcgtgctgcg cgaccagcgc ggcccgcact
     3181 cgggcgccgg ccgcctgctg ctccggcgcc gagtaggcgg ggaggcgcat gaccgtgtcg
     3241 aggtgggccg tcatggcgtc gatgaagtgg tgcgagaggt gcgctgcggc gtgccggaag
     3301 tccggctcga gcacctgctt gcggtaggcg gggtgccagc tcattcggct gctcccgtct
     3361 gctgctccgg caggtgcagg aggcgggcgg cgttgccgcc gaagatcagc tcgacttccg
     3421 cctcgctcac gccgagcgtg tagcaggcgc gcacctggtc gaggaggtag cgctcggaga
     3481 agccgcgcgg gaagaggctg gagtcggagc cgaagacgat gcgctccgcg cctaccgtct
     3541 cgagcgcctt gcggtacagg tcctcgagcg tgagcgggta gggcatccag cgcatccact
     3601 ggttggagcc cgaggtgtcg atgtagatgt tgggcaggct ccagcacagc gccagcaggt
     3661 cgtcgaagta gccggcgccg aagtggggga tcaggaacgg gatctccggg aaggcgcgcg
     3721 ccacctcgaa gagcgagagg gggctcatgc gcgggtgctg cacgatgccg ccgccgcgcc
     3781 caaggaagcc gaagtggatg agggcgggga ggcgcctctc ggcgaggaac tcccacacgc
     3841 cgcgcagctc gggcgcctcg aacgggtggt cggtgcgggg gccaagcagc ttgtagcccc
     3901 tcaggcccag ctcgtccacg gcgcgcctca gctcgacgtc ggcc
//
LOCUS       DAOQYE010000069         3929 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_23646_length_3929_cov_3.066082, whole genome shotgun sequence.
ACCESSION   DAOQYE010000069 DAOQYE010000000
VERSION     DAOQYE010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 3929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3929
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23646_length_3929_cov_3.066082"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..550)
                     /locus_tag="PKN52_02015"
     CDS             complement(<1..550)
                     /locus_tag="PKN52_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98745.1"
                     /translation="MSRTRNISSSASGLARIVGPLLLVFMALTACGGGKVTPPKGDGP
                     REASGKVALPAGAGVSLASLKAVTSLGSYPVGADGSFKAQVYGDGPVEIAVQDGAGAL
                     VLSAVSTNEPAGAALQVDGRSSAAVLLYYALGGFTLPADAQGKLWNLIRSDAAIADIE
                     AALLAAFAGGGLPMAEHDETVGN"
     gene            complement(733..3081)
                     /locus_tag="PKN52_02020"
     CDS             complement(733..3081)
                     /locus_tag="PKN52_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98746.1"
                     /translation="MARTLAPLLIALVALLTLTACGGGSKTPPPKDDEPKEVSGRVVL
                     PAGATAKPETLKAVTGLGSFAVAADGTFTAQVIGDGPTEIDVLDATGALVLSAVAGGA
                     PTTTTLDVGSRSSAEVLLYYTLGGFLLPADAQPKVWELIQKEAAVDDLADALSASFAA
                     GGLPLSDEDAALAEELDLAWQSVFGPRPMGLTGSGLKDLMTAALAPAGLLEPQAGGSS
                     NIVIEPDAGTVQSGVSVIHNPVGSGVAAQNNFRRPAALLAYQVGYQDDGGNDQAISPP
                     VLTGTVDVPSTGSLGVIQALTDIVKGEAPWSPAVSGGLPLTLHDGLRTYYQLVLLGPS
                     LDAVTHPPLYDDTRFMDERARWNEIRGDKTLELFMNDIALPILENFAFGGIGHVDASK
                     LGKLRQQFKAVNDTHLASLGIFLKAGGSYAEAVKFVLTELATNNRYRLDFFEVMVDAM
                     LESEKSKMSFEAVEARLKIRASASGIVAAVQIALSAGDVAAIMTDLNSALPAVGWTAT
                     ASPTLFALDPEHAYFTKSQPQVKLSVSTRGPVTGNFMYRWTTTGSYGELTDGPKKGTT
                     IDTVYTSVWYTVRAPHTIKDDQKDGVSVAVYEVEPGATSIPAGQAPIARMAAELSGYD
                     REIDARLEVRYGSTPPGFFLEGKTVSCATMFLHVPKEKGATKYSITFDGFGGVGNPDN
                     SNTYLFYNSSYTYVIDLNDSSTTIWPYRGVCDWRHDDDNQFYGSPAGFVARDAGDEYL
                     VSVYSNLGFLEGVPPGALDLPDKVALWYDWASQGTVKVTYSK"
     gene            3439..3768
                     /locus_tag="PKN52_02025"
     CDS             3439..3768
                     /locus_tag="PKN52_02025"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNQ98747.1"
                     /translation="MTTKRFDLAACFEPDESAPKRQLLVKEPGLTMLHLTLAPGQAMP
                     VHNHPGCHVTIQGLVGEATVWLDGEEQPLPAHELVFFSGERMVSPRNTSAAPAAVLIT
                     LVPVAGA"
BASE COUNT          605 a         1311 c         1356 g          657 t
ORIGIN      
        1 cgttgcccac cgtctcgtca tgctcggcca tgggcagtcc gccgccagcg aacgccgcca
       61 gcagcgcggc ctcgatgtcc gcgatggccg cgtcgcttcg gatgaggttc cagagcttgc
      121 cctgcgcgtc ggccggcaac gtgaagccgc ccagcgcgta gtacaggagc acggcggcgc
      181 tggaccggcc gtccacctgc agggcggcgc cggccggttc gttggtcgac accgcggaga
      241 ggaccagcgc gccagcaccg tcctggacgg ctatctcgac ggggccgtcc ccgtacacct
      301 gcgccttgaa gctgccgtcc gcacccacgg ggtaggagcc gaggcttgtg acggccttga
      361 gcgaggcgag cgagacgccg gccccggcgg gcagcgcgac cttgccactg gcctccctgg
      421 gaccgtcgcc cttgggaggg gtcaccttgc cgccgccgca ggcggtgagc gccatgaaca
      481 cgagtagcag gggcccgacg atacgtgcca agccggatgc ggaagacgag atgttacgag
      541 ttcggctcat gccgcgcagc ctagtggcgg ccctattaga gatcgcttaa taggcgtccg
      601 gcgcggcggg cgctgcttgc gaccgtcgcc cgctggccgc gacgtccgtc ggtgaccgct
      661 acggagagtg ccgagcgagc gtggcggtgg cgccgtaggt gagaggcacc accgccacgc
      721 tcgagcttca cgtcacttcg agtacgtgac cttcaccgtg ccctgcgagg cccagtcgta
      781 ccagagggcg actttgtccg gaaggtccag tgctccaggc ggtactcctt ccaagaaccc
      841 caggttggag tagacgctga cgaggtactc gtcgcccgcg tcacgagcga cgaagccggc
      901 cggactaccg tagaactggt tgtcgtcgtc atgtcgccag tcgcacacgc cacggtacgg
      961 ccagatcgtg gtgctcgaat cgttgaggtc gatcacgtac gtgtacgagg agttgtagaa
     1021 gaggtacgtg ttggagttgt cggggttccc gacacccccg aacccgtcga aagtgatgct
     1081 gtacttcgta gcgcccttct ccttgggaac gtgcaggaac atcgttgcgc agctgacggt
     1141 cttgccctcc aagaagaaac ctggcggtgt ggatccgtag cgtacttcaa ggcgcgcgtc
     1201 gatctcgcgg tcgtagccgc tgagctccgc cgccatcctc gcgatcggcg cctgtccggc
     1261 cgggatgctc gtggcgccag gctccacctc gtacactgcc actgaaacgc cgtccttctg
     1321 gtcgtccttg atggtgtgcg gcgcccgcac ggtgtaccag acgctggtgt agacagtgtc
     1381 gatggtcgtg cccttcttcg gaccgtccgt tagctcgccg tagctgcccg ttgtcgtcca
     1441 gcggtacatg aagttgcccg tgacggggcc gcgggtcgag acgctcagct tgacctgcgg
     1501 ctgggacttc gtgaagtagg cgtgctccgg atccagggcg aagagggtcg gggaggcggt
     1561 ggcggtccag ccgacggcgg ggagcgcact attgaggtcc gtcatgatgg ccgcgacgtc
     1621 accagcgctc aacgcgattt ggacggccgc cacgatgccg gaggcggagg cgcggatctt
     1681 caggcgcgcc tcgaccgcct cgaagctcat cttgctcttc tcgctctcga gcatggcgtc
     1741 gaccatgacc tcgaagaagt cgagccggta acgattgttg gtcgccagtt cggtgagcac
     1801 gaacttgacg gcctccgcgt agctgccccc cgccttgagg aagatgccga ggctggcgag
     1861 gtgggtgtcg ttgacggcct tgaactgctg ccgcagcttg ccgagcttgc tggcgtcgac
     1921 gtggccgatg ccgccgaaag cgaagttctc caggatcggc aatgcgatgt cgttcatgaa
     1981 caactcgagc gttttgtcgc cgcggatctc gttccacctg gcgcgttcgt ccatgaacct
     2041 ggtgtcgtcg taaagcggtg ggtgggtcac ggcgtcgagc gaaggtccga gcagcacgag
     2101 ctggtagtag gtgcgcaggc cgtcgtgaag ggtgaggggc agaccgccac tcaccgcggg
     2161 cgaccacggc gcttcgccct tgacgatgtc cgtcagcgcc tggatgacgc cgagcgagcc
     2221 ggtcgacggc acgtcgaccg tgccggtgag cacaggcggc gagatggcct ggtcgttgcc
     2281 gccgtcgtcc tggtagccga cctggtaggc gaggagggcg gcggggcgcc ggaagttgtt
     2341 ctgcgcggcc acgccgctgc cgacggggtt gtggatgacg gagacgccgg actgcaccgt
     2401 gccggcgtct ggttcgatca cgatattgct cgacccgccc gcctgcggtt cgagcagacc
     2461 ggccggcgcg agcgcggcgg tcatcaggtc cttgaggccg ctgccggtca gccccatcgg
     2521 ccgcgggccg aacacgctct gccaggcgag gtcgagttcc tcggcgaggg ccgcgtcttc
     2581 gtcgctcagt ggcagcccgc ccgcggcgaa ggacgccgag agcgcgtccg cgaggtcatc
     2641 gacggccgct tccttctgga tgagctccca taccttgggc tgggcgtccg cggggagcag
     2701 gaagccgcct agcgtgtagt acaggagcac ctccgcgctc gagcggctgc cgacatccag
     2761 ggtggtcgtg gtcggcgctc caccggccac cgcggagagc acgagcgcgc ccgttgcgtc
     2821 gaggacgtct atctcggtcg gtccatcgcc gatgacctgc gctgtgaagg tgccgtcggc
     2881 agctaccgcg aaggagccta gacccgtgac ggctttcaga gtttcgggct tggcggtggc
     2941 ccccgctggc aggacgaccc gaccgcttac ctctttcggc tcgtcgtcct tgggaggcgg
     3001 cgtcttgctt ccccctccgc aggcggtcag cgtgaggagc gcgacgagag cgatgagcag
     3061 cggcgctagc gtccgtgcca tgccgcggag ggcaggcggc ctttcggggg tcggggtcat
     3121 ggcgggcagc gtagagccgc ccctattaga gaacgcttaa tgaccgactt ccggtgaacg
     3181 gcctgttggt cgggtcgcct cggcgggccc ctcctccggc cggccccggg tccccgtggt
     3241 ccaacgccgc gcccctgcgc ccaatgggta catgagtcca agccgccgac ctcctcggcc
     3301 gcaactacct cggcacctgc gcccatgggt acatgggtcc aagccacccg gtgctggggg
     3361 cggacgaccc ggcggactgg ggcccatggg tacatgggtc caagccgctg aacgtgcccg
     3421 gatgccatcc tggacgacgt gaccactaaa cgcttcgacc tcgcagcctg tttcgagccg
     3481 gacgagtccg cacccaagag gcaactgctc gtcaaggagc ccggcctcac catgctccac
     3541 ctcacgctgg cccccggcca ggccatgccc gtccacaacc accccggctg ccacgtcacc
     3601 atccagggcc tggtcggcga ggccacggtg tggctggatg gggaggagca gccgctaccg
     3661 gcccacgagc tggtcttctt ctccggcgag cgcatggtga gcccgcgcaa cacgtcggcc
     3721 gcgccagccg cggtgctgat aacgctggtg ccggtagccg gagcctgagg tcactcccaa
     3781 cggctacgtc cgcctcatcc gaacgtagga tgcgcgcccg accctccccg ccctaacctc
     3841 gtggcaagga ggcgaaccat gacttacacg gatgacacgc acgaccggcc gaccgactgg
     3901 ttcctcgcgt tccagcgcag cacgccgac
//
LOCUS       DAOQYE010000070         3893 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_23943_length_3893_cov_2.949713, whole genome shotgun sequence.
ACCESSION   DAOQYE010000070 DAOQYE010000000
VERSION     DAOQYE010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 3893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3893
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23943_length_3893_cov_2.949713"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="PKN52_02030"
     CDS             <1..312
                     /locus_tag="PKN52_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 domain-containing protein"
                     /protein_id="HNQ98748.1"
                     /translation="CDASGVVIEGRFTPNEFALLSPDHLEFLRIFVKVRGNLKEVERV
                     LGLSYPTVRLRFEKLLLALDYEASEDVVEERSSILDRLEAGEIDADEAAKRLNALARK
                     G"
     gene            388..1542
                     /gene="dnaJ"
                     /locus_tag="PKN52_02035"
     CDS             388..1542
                     /gene="dnaJ"
                     /locus_tag="PKN52_02035"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HNQ98749.1"
                     /translation="MTDYYAVLEVTRDADSKTIKASYRKLAMAYHPDRNPGNRDVEEK
                     FKAINEAYAVLSDETRRARYDRYGTVDDNAVFGSDIFDIFASVFGTNVANMSGGRPRA
                     NGQPGEDLEAQLVITLEQAREGATVEVEVERLRACRHCHGERSEPGSAGKSVCQRCGG
                     AGQVRVQAQSIFGAVITTRTCPDCHGEGHVVQTPCRVCNGRGRERGTDKVSVTLPKGI
                     DGGYRLRVPREGNVGVDGGQSGDLYLYIEMRKHAFLERAGDDLLYTLEVGMAQAALGA
                     TFAIPTLEGEERLTVPPGTQPGSEFRLRGKGMPRLRQSGNGDQVVEVRVVVPTKLSEE
                     AREHLAAYAGAVEEAVDAQESVAEKVRDFFKGKRRGRGKGRDESADAASS"
     gene            complement(1549..2544)
                     /gene="miaA"
                     /locus_tag="PKN52_02040"
     CDS             complement(1549..2544)
                     /gene="miaA"
                     /locus_tag="PKN52_02040"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HNQ98750.1"
                     /translation="MSSRGSATASDVPAIPVLAGPTASGKSAAALRLALAVGEASAGA
                     GPTARHAPDTCLSVEIVSADAMQVYRGMDLGTAKPSREERAAVPHHLLDVVDPSEPFS
                     VAQYVHLAEAAIGAILARGAVPLVTGGTGFYLRALREGLPTVPPADLAAQAPMWEAVA
                     GGRLRELEAELREAAPQDAVRAAGNPRRIVRSLEVLRSTGKPPSAFPRRPPQYAYSTA
                     QLLPAMAELRPRIAARTAAMFERGLVAEVAELLRAYPEQPTALQAIGYKEVTAHLRGE
                     ASEEEARRAVELATVQYAKRQSTWFRSSAEAPALRIAATGEASHRELAAWFSELV"
     gene            2592..2984
                     /locus_tag="PKN52_02045"
     CDS             2592..2984
                     /locus_tag="PKN52_02045"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNQ98751.1"
                     /translation="MSIEKFGTAGDIQELIAVRDHIEALVEQANAEQANLPKADFLDR
                     GDAFQLHLEVPGIDQADLEIAVEDDELLVAGLREPPLVDGHMLFSERSVGPFQRSFVL
                     PSPVDRERATAHLASGVLTVTLPKLVES"
     gene            complement(2999..3772)
                     /locus_tag="PKN52_02050"
     CDS             complement(2999..3772)
                     /locus_tag="PKN52_02050"
                     /inference="COORDINATES: protein motif:HMM:NF015901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HNQ98752.1"
                     /translation="MALLAALALVGTVLAQTSTTFTVKRDDAELVVTNRGLAADGART
                     IGNNRNCSEGERMTIVYAPAPGTVETRVEDATLTSRLAIITTPDGAEAEAGEETLELS
                     GDGVTFSRPGCIDERSAAGTPSVRLVQGKTRVTGTSFFLDREADTGEMRGPIALERDA
                     GEGEEPLRATADAMEFAVGAQRATLTGNVSVVSEDRTTTGERLELDEEAGTAILTGNP
                     ARSVKGNDTLSGTRLLYYLDSNDVVVLGNVSGELDVDLQ"
BASE COUNT          580 a         1367 c         1385 g          561 t
ORIGIN      
        1 tgcgacgcca gcggcgtcgt catcgaaggg cgtttcaccc ccaacgagtt cgcgctcctc
       61 tcgccagacc acctcgagtt cctgcgcatc ttcgtgaagg tcaggggcaa cctcaaggag
      121 gtcgagcggg tcctcggcct gagctatccg accgtgaggc tgcgcttcga gaagctcctg
      181 ctcgcgctcg attacgaggc ctcggaagac gtcgtcgagg agcgcagcag catcctggac
      241 cggctcgagg ccggggagat cgacgccgac gaggcggcga agcggctcaa cgcgctggcg
      301 cgcaagggct aggtcgcggc gccccgtccg cacttcgcac cccagcactc aagaaggctg
      361 agcgtcctcc ggtatactgg cggccgcatg accgactact acgccgtctt ggaagtcact
      421 cgagacgcag acagcaagac catcaaggcc tcgtaccgca agctcgcgat ggcctaccac
      481 ccggaccgca acccaggcaa cagggacgtc gaggagaagt tcaaggcgat caacgaggcg
      541 tacgcggtgc tgtcggacga gacgcgccgc gcgcgctacg accggtacgg caccgtcgac
      601 gacaacgcgg tcttcggcag cgacatcttc gacatcttcg cctccgtctt cggcaccaac
      661 gtcgccaaca tgtcgggtgg gcgcccaagg gccaacggcc agccgggcga ggacctcgaa
      721 gcgcaactcg tcatcacgct cgagcaggcg cgggagggcg ccactgtcga ggtggaggtg
      781 gagcggctcc gagcctgccg ccactgccat ggggagcgtt cggagcccgg ctcggccggc
      841 aagagcgtct gccagcgctg cggcggggcc ggccaggtcc gcgtgcaggc gcagtcgatc
      901 ttcggcgccg tcatcacgac gcgcacttgc cccgactgcc atggcgaagg ccacgtcgtc
      961 cagacgccgt gccgcgtctg caacggtcgc ggccgcgagc gcggcacgga caaggtcagc
     1021 gtcacacttc cgaagggcat cgacggcggc taccggttgc gcgtcccgcg cgaggggaac
     1081 gtcggcgtag acggcggcca gtcgggcgat ctctacctgt acatcgagat gaggaagcac
     1141 gccttcctgg aacgggcggg cgacgacctg ctctacaccc tggaggtcgg catggctcag
     1201 gccgctctcg gcgccacctt cgcgatacct acgctcgagg gcgaggagcg cttgaccgtc
     1261 ccgccgggta cgcaaccagg cagcgagttc cggttgcgcg gcaaggggat gccgcgcctt
     1321 cggcagagcg gcaacggcga ccaggtcgtc gaggtcaggg tcgtcgtgcc taccaagctc
     1381 tccgaggaag cgcgggagca cctggccgcg tatgccggtg ccgtcgagga ggcggtggac
     1441 gcccaggaga gcgtcgccga gaaggtgcgc gacttcttca agggcaagcg gcgcgggcgc
     1501 ggcaaggggc gcgacgagtc ggccgacgca gcctcgtcct gaccagcgtc agacgagttc
     1561 gctgaaccag gcggccagct cgcggtggga ggcctcgccc gtcgctgcaa tccgcaacgc
     1621 cggcgcctcg gcgctcgagc ggaaccacgt cgactggcgc ttggcgtact gcacggtcgc
     1681 cagttcgacg gcccgccgcg cttcctcctc gctggcctcg cctcgcagat gggcggtgac
     1741 ctccttgtag ccgatcgcct ggagcgccgt gggctgctcg gggtaggcgc gcaacagctc
     1801 cgctacctcg gcgaccagcc cgcgttcgaa catcgccgcc gtgcgcgcgg cgatccgcgg
     1861 gcgcagctcg gccatggccg gcaggagctg ggcggtggag taggcgtact gcggcggccg
     1921 gcgagggaaa gcgctcggag gcttacccgt cgagcgcagc acctcgaggc tgcgcacgat
     1981 ccggcggggg ttgccggccg cccgaaccgc gtcctgcggc gcggcttccc ggagctccgc
     2041 ctcgagctcg cggaggcgcc cgcccgccac cgcctcccac atgggcgcct gggcggcgag
     2101 gtcggcggga ggcacggtcg gcaggccctc acgcagggcc cgcaggtaga accccgtgcc
     2161 gccggtaacc aacggtacgg cgccacgcgc caggatggcc ccgatggcgg cctccgccag
     2221 gtgcacgtac tgcgcgaccg agaacggttc ggacgggtca acgacgtcga gcaggtggtg
     2281 aggcaccgct gcccgctcct cgcggctggg cttggcggtg ccgaggtcca tgccccggta
     2341 gacctgcatg gcgtcggcgg agacgatctc gacggatagg caggtgtcgg gcgcgtgcct
     2401 ggccgtaggg ccggcgccgg ccgacgcctc tcccacggcg agggccaggc gcagggcggc
     2461 cgcgctcttg ccagaggcgg tcgggccggc cagcacgggt atcgctggaa cgtcggacgc
     2521 ggtcgctgag ccccgggaac tcatgcccca acgctaacaa gcggctcaaa gtccggtgct
     2581 agactcgcgc catgagcatc gagaagttcg gtaccgccgg agacatccag gagttgatcg
     2641 cggtcaggga ccatatcgag gccctagtcg aacaggcgaa cgcggagcag gcgaaccttc
     2701 ccaaggcgga cttcctcgat aggggcgacg ccttccagct ccacctcgag gtacccggca
     2761 tagaccaggc cgacctcgag atcgcggtcg aggacgacga gttgctggtg gccggactgc
     2821 gcgagccgcc gcttgtcgac ggccacatgc tcttctccga gcgcagcgtg gggccgttcc
     2881 agcgctcttt cgtcctgccg agcccggtgg atcgcgaacg ggcgacggcg cacctggcca
     2941 gcggggttct caccgtcacg cttccgaagc tcgtcgagag ctagacgacc gccgctcctc
     3001 attgcaggtc gacgtccagc tccccgctga cgttgcctag caccacgacg tcgttgctat
     3061 ctaggtagta gagcagcctg gtcccgctga gggtgtcgtt gcccttgacg ctgcgggcgg
     3121 ggttgccggt gaggatggcg gtgccggcct cctcgtccag ttccagccgc tctcccgtcg
     3181 tcgtgcgatc ctcgctcacg acgctgacgt tccccgtcag ggtggcgcgc tgggcgccga
     3241 cggcgaactc catggcgtcc gcggtggcgc ggagcggctc ctcgccttcc ccggcgtcgc
     3301 gctcgagcgc gatcgggccc cgcatctccc ccgtgtccgc ctcgcggtcg aggaagaagc
     3361 tcgtgccggt cacgcgcgtc ttgccttgca cgagccgcac gcttggggtg ccggcggccg
     3421 agcgctcgtc gatgcagccc ggccgcgaga acgtcacgcc gtcgccgctc aactccagcg
     3481 tctcctcgcc cgcctcggcc tctgcgccgt ccggcgttgt gatgatggcg agcctcgacg
     3541 tgagggtagc gtcctcgacg cgcgtctcca ccgtcccggg tgccggtgcg tagacgatgg
     3601 tcatgcgctc gccttccgag cagttgcggt tgttgccgat cgtgcgggcg ccgtccgccg
     3661 cgagcccgcg gttcgtgacg accagctcgg cgtcgtcgcg cttgaccgtg aaggtggtgc
     3721 tggtctgggc cagcacggtg cctaccaggg ccagcgccgc gaggagcgcc acccgtcgcg
     3781 accggctagg cgctggtggt cgcggcggcg acccgtccgg tcgccgatga cggcgccccg
     3841 cctgctccac caacgccagg ggcgaccgcc gcgctagcgg ctgaccgggc cgg
//
LOCUS       DAOQYE010000071         3891 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_23961_length_3891_cov_2.931178, whole genome shotgun sequence.
ACCESSION   DAOQYE010000071 DAOQYE010000000
VERSION     DAOQYE010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 3891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3891
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23961_length_3891_cov_2.931178"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            213..461
                     /locus_tag="PKN52_02055"
     CDS             213..461
                     /locus_tag="PKN52_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HNQ98753.1"
                     /translation="MNILLWIIFGALAGWIAGAITGSGKGLVTNIVVGVIGAFLGGWL
                     FNQFGGMGVTGFNLYSLLVAVVGAIVLLVIVRLFSRGG"
     gene            complement(458..1735)
                     /locus_tag="PKN52_02060"
     CDS             complement(458..1735)
                     /locus_tag="PKN52_02060"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNQ98754.1"
                     /translation="MTGAAGQSPSGAFDVVVIGGGVAGAACASRLAGAGARVLVLERE
                     RNFADRVRGDVMYPWGVAEARRLGLADALAAVVRPLPYWRTHVTGLPSTRPRDLAAEG
                     AATGADTAVAFFHPELQTALLQAAERAGARVVRGATVTGVRAGTAQRLLVEARTPGAG
                     FEVGAALVIGADGRSPRSRVWGGFKARTAEERLKFAGVMLTGAAASATSEARDTAHVF
                     FAPSAGLLAMVLPIDERRTRVYAGYHLATGERSLGGADALPAFLRLAEAAGVPHEWLA
                     AAERVGPLAQFPGADAWAANPYCNGFALVGDAAAASDPSFGCGMALAMRGARELVEAL
                     LAAGSFEPAAVDAAGEAYAEATLRTGDALRRLTGWLRDVYREPGERADALRARLWPLF
                     ARDPSRVPDVVLVGPDSPSDEAARVRFFGEDRP"
     gene            complement(1737..3074)
                     /locus_tag="PKN52_02065"
     CDS             complement(1737..3074)
                     /locus_tag="PKN52_02065"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNQ98755.1"
                     /translation="MSSDAPSLSTDLPEDGVSAAPVTAVPAVRGSLADPAVRARHARR
                     ITTALFVSQSLGTAGSIAATTVAAIVGAELSGRDSLAGLPSAVVQIGVAIGAYFWSRR
                     SDRLGRRGALVAALLTGMVGATMSLFAVASGNFPLVLVALFITGSGNSAVQLGRFVAA
                     EVNPRARRARALSTVVFGGTIGSVVGPALVGPTGRLVEGWGWSEIAGPYLATGVGYLL
                     TAVLLFLALRPEPRLIGDAIAFDEGTTVRTGSARSLRQLLADTAVRTAVASVVLAHMV
                     MVGLMQMTSLHMHQHAHSLANISFVFSSHTLGMYAFSTVSGWMTDKWGRRRVLGIGAV
                     IIIASCLLAPLSPHLLPIAFALFLLGLGWNLCYVAGSALLTDALTPAEKGRMQGFNDL
                     LVGGAAASATIVGGLIMANAGYLAMGLAGAAVSAPLLLIVMRLPRPATGAALD"
     gene            complement(3184..3663)
                     /locus_tag="PKN52_02070"
     CDS             complement(3184..3663)
                     /locus_tag="PKN52_02070"
                     /inference="COORDINATES: protein motif:HMM:NF021520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HNQ98756.1"
                     /translation="MTGATPQTHDRQAPTPSEAVAALNKLFDKALRALGDAGRQDDAC
                     ELAAQGWSLLRQEWPKEGERLNGTLHYLTRTLKPKPVTDAGSDRLLEVRHLPPAERHR
                     LIFQTYADLAPGEAFLLINDHDPKPLYYQLAAENPGAFSWEPVEEGPEVWRVRIGKV"
BASE COUNT          611 a         1367 c         1412 g          501 t
ORIGIN      
        1 gttcgaaccg acagtccaaa ccgagtcacc ggcgaacgcg gaaacgggcg cgaccgacag
       61 cgggctagag cgcggctgag agtggcgcgc gcaagcgccg ggcctcggcc ggggccgccg
      121 acaggtcatc cgtaccaaga aaacgccgtt cctactgcta cctttgtcga gcgccggtcg
      181 gagtaaggac cgcgccggaa aggaaccacc agatgaacat cttgctatgg atcatcttcg
      241 gcgcgttggc gggctggatc gcgggtgcca tcacgggctc cgggaaaggg ctcgtcacca
      301 acatcgtcgt gggcgtcatc ggcgccttcc tgggcgggtg gctgttcaac cagttcggcg
      361 gcatgggcgt cacgggcttc aacctctaca gcctcttggt ggcggtcgtc ggcgccatcg
      421 tcctgctcgt catcgtgcgg ctgttctccc gaggcggcta gggccgatcc tccccgaaga
      481 accggacccg ggcagcctcg tcgctcggcg agtcaggtcc gaccaggaca acgtcaggga
      541 cgcggctggg gtcgcgcgcg aacaggggcc agagtcgagc acgtagcgcg tccgctcgct
      601 cgcccggttc cctgtacacg tcccgcaacc aacccgtgag cctgcgcagg gcgtcgccgg
      661 tacgcagcgt agcctcggcg taagcctcgc cggcggcgtc gaccgccgcc ggctcgaacg
      721 agcccgcggc cagcagcgcc tccactagtt ctcgtgcccc acgcatggcg agggccatcc
      781 cgcagccgaa gctcggatcg ctcgccgctg cagcgtctcc gacgagggcg aaaccgttgc
      841 agtacgggtt cgcagcccat gcgtcggcgc cggggaactg cgccagcgga cccacccgct
      901 cggctgcggc cagccactcg tgcggcacgc ccgccgcctc cgcgaggcgc aggaaggccg
      961 ggagggcgtc cgcgccaccg aggctccgct cccccgtcgc gaggtggtaa cccgcgtaca
     1021 cgcgcgtcct acgctcgtcg atcggtagca ccatggcgag gagaccggcg ctcggcgcga
     1081 agaagacgtg ggcagtgtcg cgtgcctcgg acgtcgcgga ggcggcggcg cccgtgagca
     1141 tcaccccggc gaacttcagc cgttcctcgg ccgtgcgcgc cttgaaccct ccccagaccc
     1201 gggagcgcgg cgagcggccg tcggcgccta tgacgagggc ggcacctacc tcgaacccag
     1261 cgcccggggt cctcgcctcg accagtaacc tctgcgcggt cccggcacgg acgccggtga
     1321 ccgtggcgcc ccgcacgacg cgggcgccag cccgttctgc ggcctgcaac agcgccgtct
     1381 gcagttcggg gtggaagaag gcgacggccg tatctgcgcc ggtagcggcg ccctcggcgg
     1441 ccaggtcgcg cgggcgggtc gacggcaggc ctgtgacatg cgtccgccag tacgggagcg
     1501 gtcgcaccac ggccgcgagc gcgtccgcca agccgaggcg ccgcgcctca gccacgcccc
     1561 acgggtacat gacgtccccg cgcacgcggt cggcgaagtt acgctcgcgc tccagcacga
     1621 gcactcgggc gccggcgccg gccagacgcg aggcgcaggc cgcgccggcc acgccgcccc
     1681 cgatcacgac gacgtcgaac gcgccgctgg gcgactggcc agcggcacct gtcacgtcag
     1741 tcgagcgcgg cgcccgtggc ggggcgcggc agtcgcatca cgatgagcag cagtggcgcg
     1801 gaaacggccg cccccgcgag acccatggcc aggtagccgg cgttggccat gatcaggccg
     1861 ccgacgatcg tcgcgctggc ggctgccccg cccacgagca ggtcgttgaa gccctgcatg
     1921 cggcccttct cggccggcgt cagcgcgtcg gtgaggagcg cggagccggc tacgtagcag
     1981 aggttccagc caaggccaag aaggaagagc gcgaacgcga tcggcaggag gtggggcgac
     2041 aggggtgcga gcaaacacga cgcgatgatg atgacggccc cgatgccgag cacgcggcgc
     2101 cttccccact tgtctgtcat ccagccagaa acggtcgaga acgcgtacat gcccagcgta
     2161 tggctggaga acacgaacga gatgttggcg aggctgtggg cgtgctgatg catgtgcagc
     2221 gacgtcatct gcatgaggcc gaccatgacc atgtgggcga gcacgacgct cgcgaccgcc
     2281 gtacgcaccg cagtgtccgc caggagctgg cgcaggctcc gcgcgctgcc cgtgcgcacg
     2341 gtcgtgccct cgtcaaacgc gatggcgtcg ccgatgaggc gcggctccgg ccgcagggcc
     2401 aagaagagga gcacggcggt gagaaggtag ccgacccccg ttgccaggta gggaccggcg
     2461 atctcgctcc agccccaccc ctcgacgagg cgtccagtcg gtccgacgag ggccggccca
     2521 acgacgctgc cgatggtgcc tccgaacacg acggtggaaa gagcacgggc tcgcctggcg
     2581 cgcgggttca cttcggcggc cacgaagcga ccgagttgca cggccgagtt gccagaaccg
     2641 gtgatgaaga gggcgacgag caccaggggg aagttgccgc tggctacggc gaagagcgac
     2701 atggtggcgc ccaccatgcc cgtgagcagc gcggcgacga gggcgccgcg gcgacccagc
     2761 cggtcggagc gccggctcca gaagtaggcg ccgatggcca cgccgatctg gacgacggcg
     2821 gacggcagac cagccagcga gtccctaccg gagagctcgg cgccgacgat ggccgccacg
     2881 gtggtggccg cgatggaccc ggccgtgccg agactctggc tgacgaacag ggcggtcgtg
     2941 atgcgccgcg catggcgagc tcgcacggcc ggatcggcca gcgacccgcg aaccgccggc
     3001 accgcggtca ccggggcggc ggacacgccg tcctcgggca gatcggtcga caggctcggg
     3061 gcgtcgctgc tcaccgcgcc agactacccg aaagccgcta acggccggcg ctaccgccgc
     3121 ggcgcgacgt agccagcgcc tgcggccggg acgggcctgg gagccctctc cggcggcgcc
     3181 cgcctagacc ttgccgatcc tcacccgcca gacctccggg ccttcctcca ccggctccca
     3241 gctgaaggcg ccggggttct cggcggcgag ctggtagtag agcggcttgg ggtcgtggtc
     3301 gttgatgagc aagaaggcct cgcctggtgc gaggtcggcg tacgtctgga agatgaggcg
     3361 gtggcgctcg gccggaggga ggtgcctaac ctcgaggagg cggtcggagc cggcgtcggt
     3421 gaccggcttg ggcttgagag tgcgcgtgag gtagtgaagc gtgccgttca ggcgctcgcc
     3481 ttccttaggc cactcctggc gcagtaggga ccaaccctgg gcggccagct cgcaggcgtc
     3541 gtcctgcctg ccggcgtcgc cgagggcgcg cagggccttg tcgaagagct tgttcagcgc
     3601 ggcgacggct tcgcttgggg tcggtgcctg gcgatcgtgg gtttgtgggg tggctcccgt
     3661 catgccggcg accctaactg cggccaccgg cgcggagcca tgacccgtga caaagggccc
     3721 ggcgcggcta gcgctcaggc atgaaccaac ccgaagacgt cctcgacctc ggcgaccgct
     3781 acatcgggat cgccgcacct caccgtggtg agacccagcc cggacgccgc cgcgacgttc
     3841 tcgggcaggt cgtctatgaa gagcgcctgc tcgggcgcca cgccctcggc a
//
LOCUS       DAOQYE010000072         3812 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_24738_length_3812_cov_2.707746, whole genome shotgun sequence.
ACCESSION   DAOQYE010000072 DAOQYE010000000
VERSION     DAOQYE010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 3812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3812
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24738_length_3812_cov_2.707746"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            79..945
                     /locus_tag="PKN52_02075"
     CDS             79..945
                     /locus_tag="PKN52_02075"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNQ98757.1"
                     /translation="MDLALLPQAIVSGVLASGLYALVSVGLALAIGVIGIVNFAHGEF
                     FMVGAFLAYQLFVSFGFDPLLSLLFVAPALAVIGALIYLSSIRFVLKAPELNQMLLTF
                     GLGIILQNLALLIWGGDPRTIANVPYRSLGFQVGGVSIGAVPLGSFVISLVLVAGLYW
                     VLAATPLGRAMRAVAQNRIGAGLVGLEVNRVYLVAFALSALLAGLGGVMIGVIQSPTP
                     TVGLGFTLKAFAIVVLAGLGNIRGIVGAALTVALAESLVATLVPNGDALRNMVFFAII
                     FVVLVWRSRRVA"
     gene            942..2033
                     /locus_tag="PKN52_02080"
     CDS             942..2033
                     /locus_tag="PKN52_02080"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNQ98758.1"
                     /translation="MRGIARDLVVTVAFAILLAFLPGLLRELFGRASVSYLSLATNAL
                     VLAVLALSWDMLARTGQLSLAHAAFTGAGGYTVAVLFKLTGAPLWLGIPLGGLVAAAL
                     ALGLGSVTLRLFGIYFAIATLAFTEVLKAAVQRLPTAVAGGAAGINVPALFRPTFVPG
                     TMERWEVAFFRNQGYFWVYAGLLVAALVISLVVQRSRLRAAFTALRTNEQVAGVMGVN
                     PARMKLLAFVLSSFLAGLLGSVEAHRLGSVSPDGAFSVHTTVLALVTPIFGGLYTTLG
                     PILGATVLAGIEETLKRTFSEGYLIGYGVVLVASILFMPRGLVGLIGKLFRGRGAGGA
                     GRAGALADARGASGTDGARGVDDPAEERA"
     gene            2030..2851
                     /locus_tag="PKN52_02085"
     CDS             2030..2851
                     /locus_tag="PKN52_02085"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ98759.1"
                     /translation="MSTVLRVTKLSKSFGGLVAVKDVSFEAAQGEIFAIIGPNGAGKT
                     TLLNLISGLLTPTAGTLELLGTDVTTMPTYRRCRLGLGRAFQVVQPFPEMTVAENVLV
                     GAQFGSDGVSSAEARRRTDRALERTGLAPLRDQPVEDLNLLQEKRLEIARALATQPKV
                     LLLDEVMAGLRPGEARQAVELVRSVRDEGITVVFIEHVMPVVRDLADRVLVMDHGAEL
                     ARGTYADVTSDPRVVEAYLGVEDEDAQGGDEPVGVPSVGASSTADDAGGTTGGSA"
     gene            2852..3571
                     /locus_tag="PKN52_02090"
     CDS             2852..3571
                     /locus_tag="PKN52_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ98760.1"
                     /translation="MGRALEISGLRAGYGKITVLWDVDLHVEEGELVTIIGANGAGKT
                     TLLRTISGLVQARSGKVNAFGSSSLVGWSAARIVRAGVGHVPEGRQLFPLMTVRENLE
                     SGAEYLSRAKPNAARNRKLVFDLFPRLAERGAQLAGTLSGGERQMLAIGRALMSDPRL
                     LLVDEPSLGLSPAPSLTVFRALEQINAEGVTVVLVEQNVQQSLRIADRAYVLENGEVA
                     LQGTGAELLLNPAVREAYLSM"
     gene            complement(3586..>3812)
                     /locus_tag="PKN52_02095"
     CDS             complement(3586..>3812)
                     /locus_tag="PKN52_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent deacetylase"
                     /protein_id="HNQ98761.1"
                     /translation="MRWLEGADALLVLGSSLEVFSGYRFVKAAVVAGKPVAIVNQGPT
                     RGDAAAALRLEAGLGEVLPAAVALAAGGAA"
BASE COUNT          501 a         1384 c         1308 g          619 t
ORIGIN      
        1 gagcagctag cccgaaggcg ccggggggct gcgcgagcat gcgcggcccc ccgaccgccc
       61 tcacggcacg agaccaccat ggacctcgca ctcctaccgc aagccatcgt ctcgggcgtg
      121 ctcgcctccg gcctgtacgc gctcgtgtcg gtcgggttgg cgctcgccat cggcgtgatc
      181 ggcatcgtca acttcgccca cggcgagttc ttcatggtgg gagcgttcct cgcctaccag
      241 ctcttcgtga gcttcggctt cgacccgttg ctctcgctcc tcttcgtggc gcccgccctc
      301 gcggtcatcg gcgcgttgat ctacctatct tccatccgct tcgtgctgaa ggcgcccgag
      361 ctcaaccaga tgctcctgac cttcggcctg ggcatcatct tgcagaactt ggcgctcctc
      421 atctggggcg gcgacccgcg caccatcgcc aacgtgccct accgctcgtt gggcttccag
      481 gtcggcggcg tgagcatagg cgccgtgccc ctcgggagct tcgtcatctc gctcgtgctc
      541 gtggccggcc tctactgggt gctcgcggcc acgccgctgg ggcgcgccat gcgagccgtc
      601 gcccagaacc gtatcggggc gggcctcgtc gggctcgagg tgaaccgcgt ctacctcgtc
      661 gccttcgccc tctctgccct cctcgcaggc ctcggcggcg tgatgatcgg cgtcatccag
      721 tcacccaccc ccacggtggg gctcgggttc acgctcaagg cgttcgccat cgtggtcctc
      781 gccgggctcg gcaacatccg gggcatcgtc ggtgcggctc ttacggtcgc gctcgcggag
      841 tcgctcgtcg cgacgctcgt acccaacggc gacgccctgc gcaacatggt cttcttcgcc
      901 atcatcttcg tcgtgctcgt atggcggtcg cggagggtcg catgaggggc atcgcgcgcg
      961 acctcgtcgt cacggtggcg ttcgccatcc tgctcgcgtt cctccccggg ctcctccgcg
     1021 aactcttcgg ccgagcctcg gtgagctacc tgtcactcgc caccaacgcg ctcgtgctcg
     1081 ccgtgctggc actgtcgtgg gacatgctcg cgcgcacggg ccagctctcg ctcgcccacg
     1141 ccgccttcac gggcgccggg gggtacacgg tcgcggtgct cttcaagctc acgggcgcgc
     1201 cgctctggct cggcatcccg ctcgggggcc tcgtcgccgc cgccctggcg ctcggcctcg
     1261 gctcggtcac cctcaggctc ttcggcatct acttcgccat agcgaccttg gcgttcaccg
     1321 aggtcctcaa ggccgccgtg cagcgcctcc cgacggcagt ggccggcggg gcggccggca
     1381 tcaacgtgcc ggcgctcttc cgcccgacgt tcgtgcccgg caccatggag cgctgggagg
     1441 tcgccttctt ccgcaaccag gggtacttct gggtgtacgc cgggctgctg gtggctgccc
     1501 tcgtcatctc gctggtcgtc cagcgctcgc gcctgcgggc ggcgttcacg gccctgcgca
     1561 cgaacgagca ggtggccggc gtgatgggag tgaacccggc acgcatgaag ctcctcgcgt
     1621 tcgtcctctc gagcttcctc gccggcctgc tcggcagcgt ggaggcgcac cgcctcggga
     1681 gcgtgagccc tgacggcgcc ttctccgtcc acacgacggt cctcgccctc gtcacgccca
     1741 tcttcggcgg cctctacacc accctcggcc ccatcctcgg cgcgaccgtc cttgccggca
     1801 tcgaggagac cctcaagcgc accttctccg aggggtacct gatcggctac ggggtcgtcc
     1861 tcgtggcgtc catcctgttc atgccgcgcg ggctcgtcgg gctgatcggc aagctcttca
     1921 ggggccgcgg ggcgggaggc gctggacggg ccggcgccct ggccgatgct cgtggcgcca
     1981 gtggcaccga tggggccagg ggcgtcgacg acccggcaga ggagcgcgca tgagcaccgt
     2041 cttgcgtgtc acgaagctga gcaagtcgtt cggcgggttg gtggcggtca aggacgtgtc
     2101 attcgaggcg gcgcaaggcg agatcttcgc gatcatcgga cccaacggcg ccggcaagac
     2161 tacgctcctc aacctgatct ccggtctcct cacgcctacg gccggcaccc tcgagcttct
     2221 cggcaccgac gtcacgacca tgccgacgta ccggcgctgc cggctggggc tcgggcgcgc
     2281 cttccaggtc gtgcagccgt tcccagagat gacggtggcg gagaacgtct tggtaggggc
     2341 gcagttcggc tccgacggcg tcagtagcgc cgaggcgcgg cgccgcacgg accgggcgct
     2401 ggagcgcaca ggtctcgcac ccctacgtga tcaacccgtc gaggacctca acctcctcca
     2461 ggagaagcgc ttggagatag cgcgggcgct cgccacccaa ccgaaggtgt tgctgctcga
     2521 cgaggtgatg gcgggtctgc gccccggtga ggcccgccaa gcggtggagc tcgtgcgcag
     2581 cgtgcgcgac gaggggataa ccgtggtgtt catcgagcac gtgatgccgg tcgttcgcga
     2641 cctcgccgac cgcgtgctcg tcatggacca cggcgcggag ctcgcgcgcg gcacgtacgc
     2701 cgacgtgacg agcgaccccc gcgtcgtgga ggcgtacctc ggcgtcgagg acgaggacgc
     2761 gcaggggggc gacgagccgg tcggcgtccc gtcggtaggt gcgtcgtcga ccgctgacga
     2821 cgcggggggc acgacgggag ggagcgccta gatgggacgg gcactcgaga tcagtggcct
     2881 gcgggccggg tacggcaaga tcacggtcct ctgggacgtc gacctgcacg tggaagaggg
     2941 cgagctggtc acgatcatcg gggccaacgg cgcgggcaag acgacgctgc tccgcaccat
     3001 cagcggtctc gtccaggcgc gctcgggcaa ggtgaacgcc ttcggctcct cctcactcgt
     3061 cggctggagc gcggcacgca tcgtccgcgc cggcgtaggg cacgtgccgg aggggcgcca
     3121 gctcttcccc ctcatgacgg tccgcgagaa cctcgagagc ggggcggagt acttgagccg
     3181 cgccaagccc aacgcggcgc gcaaccgcaa gctcgtcttc gacctgttcc cgcgcctagc
     3241 ggagcgcggc gcccagctgg ccggcacgct ctcgggcggg gagcggcaga tgctcgccat
     3301 cggtagggcg ctgatgagcg acccgcggct actgctcgtc gacgagccgt cgctcggcct
     3361 gtcgccggcc ccgtcgctca ccgtgttccg cgccctcgag cagatcaacg cggagggcgt
     3421 gaccgtcgtg ctggtggagc agaacgtgca gcagtcgctg cggatcgccg accgcgccta
     3481 cgtgctcgag aacggcgagg tcgcgctgca ggggacgggc gcagagttgc tgctgaaccc
     3541 cgccgtgcgc gaggcgtacc tgtcgatgtg acgccctcgg cccactcagg cggccccgcc
     3601 cgccgccagg gccacggcgg cgggcagcac ctcgcccaac cccgcctcca gccgcaaggc
     3661 ggcagcggcg tcgccgcgag tcgggccctg gttgacgatg gccacgggct tgccagcgac
     3721 gacggccgcc ttgacgaacc ggtagccgga gaagacctcc agcgacgaac cgagcacgag
     3781 cagggcatcg gccccctcca accagcgcat gg
//
LOCUS       DAOQYE010000073         3810 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_24756_length_3810_cov_3.098535, whole genome shotgun sequence.
ACCESSION   DAOQYE010000073 DAOQYE010000000
VERSION     DAOQYE010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 3810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3810
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24756_length_3810_cov_3.098535"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="PKN52_02100"
     CDS             complement(<1..412)
                     /locus_tag="PKN52_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98762.1"
                     /translation="MIVHDPFGNDDPYKSSPAERFPRDPAPGDAVQVGFRVPAGAREA
                     WAEVKHARPGGQTDTRRAVATRLAGDAWAVDLGAFADGAVEYVLRAVADGQAGSDVGS
                     GAWSSAGSRAGEDPGSGPASRAADDPISAGPYRFD"
     gene            complement(544..1794)
                     /locus_tag="PKN52_02105"
     CDS             complement(544..1794)
                     /locus_tag="PKN52_02105"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HNQ98763.1"
                     /translation="MKSHKASRWMTRLAFALALGLAAFGTAQTVNYFSFTTGSDKLDV
                     LEHLIDVFESENPGITINYTTADFGSYFTKLQTDFAAGTSPDVFELNYENFVTFASRG
                     TLRDLRPDIASSSLIGDGTFYPAALDAFAYDGAQLGLPITFSTVLLIYNKDMFDAAGV
                     SYPTDDWTWDDVIAAGKAISDPANRVWGISQTVQFWEFYKAAAQAGGGLTVTPTVQID
                     TPENRAAAHYLVDKVQLHHIMPTDAEMSGVGDIDLFLNQQLGMIVTGIWMFDNFVTNA
                     TFNWDVAVEPGGVQKATHFFSNAAVVSSQSKVPDAAYKWVEFLAAHPAVVQARIETNW
                     ELSALSLEQADALEPYLAKPVPANREAVFESLAYAVNPPVVENQPELQDIVNQELEAA
                     RLGTKTVEQALQDAQKRVEALVGR"
     gene            complement(1851..3077)
                     /locus_tag="PKN52_02110"
     CDS             complement(1851..3077)
                     /locus_tag="PKN52_02110"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HNQ98764.1"
                     /translation="MRNGSTSALGREKIRLLNQSAIINAIHRSRKVSRTDLAAQLRLS
                     PAAVTNLTASLIDAGLVVEVEVGESQSVGRKPILLGINYDHSYVVGAKVMPDAIVASL
                     TNLNADVLGTVRVPLADSAVGTVAAAVVASKAELCGKLGVSAHRVAGLGVSLPGVVDY
                     LTGIVRHSDLLGWHDVRLAEELHAVAGLSAVVENDVNALAAAQSWFGLGRAHDSFLTV
                     TMGRGVGLGIVIGGAIYRGPRGGAGEVGHTRCTPIAPRVMRPGTTTLEERLGDAGLLA
                     EASRVIPGFPEGARPEALTALAERGDPAAIDLLADAGECLGVALSDLVNLFAPTLVIL
                     GGEGLRNSKHFLPHVRPALEQHAFGGLAEGLELIVDSWGDDAWARGAAGLAAARFLEA
                     ATEPLPALQTGPGLAR"
     gene            complement(3236..3646)
                     /locus_tag="PKN52_02115"
     CDS             complement(3236..3646)
                     /locus_tag="PKN52_02115"
                     /inference="COORDINATES: protein motif:HMM:NF024861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HNQ98765.1"
                     /translation="MGSVRAFLDANVLFSAALGGPAFALLWELAGAGRVRLVTSVYCH
                     VEAERNLERKYPARLSYLATRMQRVLVVPDVRDHVVPGGLLAQKDVPVYAAAVAARAA
                     VLLTGDTKHFSHLMERSDLPLRVMTVARFLRTGY"
BASE COUNT          585 a         1259 c         1345 g          621 t
ORIGIN      
        1 cgtcgaagcg atatgggccg gcgctgatgg ggtcgtccgc ggctctcgat gccgggcctg
       61 atccgggatc ttccccggcg cgcgacccgg cgctcgacca cgcgccagac ccgacgtccg
      121 agccagcttg tccgtccgcc acggcgcgca agacgtactc caccgcaccg tcggcgaagg
      181 cgccgagatc gaccgcccac gcgtcgccgg cgagcctcgt tgcaacggcg cgcctcgtgt
      241 cggtctggcc acctggcctg gcatgcttga cctccgccca ggcctcgcgc gcgccggcgg
      301 gcacacggaa gccgacctgt acggcgtcgc ctggcgccgg gtcgcgtggg aagcgctcgg
      361 cagggctgct cttgtaaggg tcgtcgttac cgaacggatc gtgcacgatc atcgggcgtt
      421 cagcctcctg gcggtggggg gcgccgcgca ccaacggcgg cgcccccgtc gggcggttag
      481 tcctaacgtc tcggctagca ctgcctgtgg ccgggcacca acgcaggccc gacgccgcca
      541 gctctaccgg cctaccagcg cctcgacgcg cttctgcgcg tcctgcaggg cctgttcgac
      601 ggtcttggtg ccgaggcgcg cggcctcgag ttcctggttg acgatgtcct ggagctctgg
      661 ctggttctcg acgacgggcg ggttgacggc gtaggcgagc gactcgaaga cggcttcgcg
      721 gttcgccgga acgggcttgg cgaggtacgg ctcgagcgcg tcggcctgct cgagcgagag
      781 ggccgagagt tcccagttcg tctcgatgcg cgcctgtacg acggccggat gagcagcgag
      841 gaactcgacc catttatagg cggcgtcggg caccttgctc tggctcgata cgacggcggc
      901 gttggagaag aagtgcgtag ccttctgcac cccgcccggc tcgaccgcga catcccagtt
      961 gaaggtggcg ttcgtgacga agttgtcgaa catccagatg cccgtgacga tcatgccgag
     1021 ctgttggttg aggaacaggt cgatgtcgcc gacgccgctc atctccgcgt ccgtgggcat
     1081 gatgtggtgg agttgcacct tgtcgaccag gtagtgcgcc gccgcgcggt tctccggggt
     1141 gtcgatctgc acggtggggg tcacggtgag gcccccgccc gcctgggcgg ccgctttgta
     1201 gaactcccag aactgcacag tctgggagat gccccagacg cggttggccg ggtcgctgat
     1261 ggccttgcct gccgcgatga cgtcgtccca cgtccagtcg tccgtcgggt agctcacgcc
     1321 ggccgcgtcg aacatgtcct tgttgtagat gaggagcacc gtggagaacg tgatcggcag
     1381 cccgagctgg gcaccgtcgt aggcgaaggc gtcgagggcg gcgggataga aggttccgtc
     1441 gccgatcagg ctgctgctag cgatgtccgg gcgcaggtcg cgcaacgttc cgcgggaggc
     1501 gaaggtgacg aagttctcgt agttgagctc gaagacgtcg ggcgaggtgc cggccgcgaa
     1561 gtcggtctgg agcttcgtga agtacgagcc gaagtcggcg gtcgtgtagt tgatcgtgat
     1621 gccggggttc tccgactcga acacgtctat gaggtgctcg agcacgtcca gcttgtccga
     1681 gccagtcgtg aacgagaagt agttgaccgt ctgcgccgtg ccgaacgcgg ccaggccgag
     1741 cgccagcgcg aacgccaacc tcgtcatcca acggcttgcc ttgtggctct tcatgacttc
     1801 ctccttcgcc tcgctgggct tcttgctgct gagtcgtcgc cgacgagccg tcatcgtgcg
     1861 agaccgggcc cggtctgcag cgccggcaac ggctcggtcg cggcctcgag gaagcgtgct
     1921 gcggcgaggc cggcggcccc gcgtgcccac gcgtcatcgc cccacgagtc gacgatcagc
     1981 tcgaggccct cggctagacc gccgaaggcg tgctgttcga gcgccggtcg cacgtggggc
     2041 aggaagtgct tcgagttgcg caggccttcc ccgccgagga tgacgagggt aggcgcgaag
     2101 aggttgacaa ggtcgctcag cgctacgccg aggcattcgc cggcgtcggc gagcaggtcg
     2161 atggccgcag ggtcgccccg ctcggccagc gctgtgagcg cctccggacg cgcgccctcg
     2221 ggaaacccgg gaatgacgcg actggcttcg gccagcaggc ctgcgtcgcc gagccgctcc
     2281 tccagggtcg tggtgccagg ccgcatgacg cgcggggcga taggggtaca gcgggtgtgc
     2341 ccgacctcgc cggcgccgcc gcgcggaccg cggtagatgg cgccgccgat cacgatgccc
     2401 agaccgaccc cgcgccccat ggtgacggtc aggaaggagt cgtgcgcccg cccgaggccg
     2461 aaccagctct gggcggcggc gagggcgtta acgtcgttct cgaccacggc ggataggccc
     2521 gccactgcgt ggagctcctc ggcgagccga acgtcgtgcc aaccgagcag gtccgagtgc
     2581 cggacgatcc ccgtcaggta atcgacgaca ccgggcaggc tgacacccag tccggccacg
     2641 cggtgagcgg acacgccgag cttgccgcag agttcggcct tggaagcgac cacggcggcg
     2701 gcgaccgtgc cgaccgccga gtcggcgagt gggacgcgca cggtgccgag cacgtcggcg
     2761 ttgaggttcg tcaacgacgc cacgatggcg tcgggcatca ccttggcgcc caccacgtag
     2821 gagtggtcgt agttgatgcc gagcaggatg ggtttgcgcc ccaccgattg ggactcgccc
     2881 acttcgacct cgacgacgag cccggcgtcg atgagggagg ccgtcaggtt cgtgacggcg
     2941 gctgggctga gacgcaactg cgcggcaagg tcggtcctcg agaccttcct ggacctgtgg
     3001 atcgcgttga tgatcgcgct ctggttgagt aaccggatct tctcgcgacc gagtgcgctg
     3061 gttgagccgt tccgcatggg tccgcccttt agttcatttg gtgtagcaag tattgatcca
     3121 tgcgtctcgg gttgtcaagg ccaccgggcg cgcaccagtc gacgcgctcc gtgatgtagc
     3181 gcgtccggcg cctccagcac gacgaacgcc ggagcccggc gacggcctta ccggctcagt
     3241 agcctgttct gaggaagcgc gcgaccgtca tcaccctgag cggcaggtca gatcgctcca
     3301 tcaggtgaga gaagtgcttc gtgtctcccg tgaggagaac ggcggcccgc gcggccacgg
     3361 ccgccgcata aacgggcacg tccttctgcg cgaggagacc gccgggaaca acgtggtcgc
     3421 gaacgtccgg gacgaccaga acgcgttgca tcctggtcgc gaggtagctc aggcgagcag
     3481 ggtacttgcg ctccaggttg cgctcggcct cgacgtggca gtacacgctc gtgacgaggc
     3541 gcacccttcc ggcaccggcg agctcccaga gcagcgcgaa cgccggaccg ccaagcgcag
     3601 cgctgaacaa gacgttggcg tctaagaagg cccttacaga ccccatcgag cgcgcgcctc
     3661 ggcgagttcc tcttccgtga gctcgccggc ggccaggaac tccgcgatcc gctcgtccga
     3721 gtacagctcg accggcatga cctcaacggg ctgcaggacg atgcgcccct cctcgatgtc
     3781 gatgccgagg ctcgatccgg acaggaccgc
//
LOCUS       DAOQYE010000074         3797 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_24861_length_3797_cov_2.952966, whole genome shotgun sequence.
ACCESSION   DAOQYE010000074 DAOQYE010000000
VERSION     DAOQYE010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 3797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3797
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24861_length_3797_cov_2.952966"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(206..1087)
                     /locus_tag="PKN52_02120"
     CDS             complement(206..1087)
                     /locus_tag="PKN52_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98766.1"
                     /translation="MNDFDTLSGVLASAVATSGTFTTGYPSGGRIGDYRSGKAHKLIV
                     GQKEYSSPNDFGVAYTSATVITITWRAANTLPAGSAFRLQLDRGGGAFVDGRQDRGPP
                     GVNNASYAGVVRVNLGAPITADVDGVAAVQLLGSATDFVLDGARVVDGVAILDVPRNI
                     TLTVATTDHSGKTITVYGTDEYGEELIESRAGPNNNTVALAKAFKTVTRVASSAAIAT
                     NGISVGFGDGIWRSRPPSSRERALIGKRGICRKRGHWVGSYRGQVLHSARLQVLVHRT
                     AGADVKDGAGDAAWSYR"
     gene            complement(1105..2151)
                     /locus_tag="PKN52_02125"
     CDS             complement(1105..2151)
                     /locus_tag="PKN52_02125"
                     /inference="COORDINATES: protein motif:HMM:NF033394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HNQ98767.1"
                     /translation="MPFTALQLENIANASLDYYLKGQPLSQTIQEKPLMGAMKGVQKT
                     FPGGKGEIKGNVKADYLTTFVGYSHDDTVTYGNPANIKQYSYSWKELHAGLSVTLTEL
                     KHDGISVTDSTTGESTSNHSEREKVAISNLLQDKLEDMDEGMARSMNEMMWLDGTQSS
                     KVFAGIQHFIAKDPTTGIVGGIDRSQNPWWRNRAATGAGKITHSAANQTLTKFLRAEN
                     RQLRRYGGRPSLVLAGSGFIEKLEAEIHEKGTYTMEGFLKAGATEIGMADISMRGVGK
                     IQYDPTLDDLDEENFAYFIDTRHLYPYVMDGEDMKKHSPARPPSQYVLYRGVTWTGTV
                     ICRKMNCHAVYEAA"
     gene            complement(2171..3229)
                     /locus_tag="PKN52_02130"
     CDS             complement(2171..3229)
                     /locus_tag="PKN52_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98768.1"
                     /translation="MLEHDSEASPASENGDEAPAASLDETSAGASPAAGVTEGSPPSD
                     DKEAPPKSLLDVISNVVEPAKMEAPEETSASETVTSDDAKAPEGEQELSEEELAKLPF
                     GKHPRFKKVLGERNAFKQQASEFQAQVQELEGPAGQYQRIDGFLRNAGIQPAEFVELM
                     QVGALLKNNPEAARQELIRRLIDLDKITGHALPEDLRKDVEEGRLEEQRARELSIARA
                     REARATETAKVATTHARHVEQSTQVERFQGEVVDAVSGWEQRTRASDPDFAAKEELIA
                     DRVIALNARLGPPKSAEDAVRRAEQAHKAVTDQLKGMLPPKPEVVRRNPGSHSTSNVA
                     ADPKSMLDVVNIALASGA"
     gene            complement(3287..>3797)
                     /locus_tag="PKN52_02135"
     CDS             complement(3287..>3797)
                     /locus_tag="PKN52_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98769.1"
                     /translation="IDDLDDFLSILARLSGQVLLREMSEPIVKQVCGPGAVWPTLTNQ
                     QIAEELYLEIQAGSSGRPNKERDLANFERVAPTMVQIPGINPEWFAKYLIQLLDNTID
                     WEEAVTAGLPAIVSMNRMTQPGAEDPANSPDAQGEQGGQNAPNDDTSTGGPQAALPAP
                     DAAQPNVMA"
BASE COUNT          577 a         1188 c         1276 g          756 t
ORIGIN      
        1 gaccgtcaaa ggggtcgctg aaaccgtccg tcgctcgata atcctcacgc ccggcggatc
       61 cgctacgccc ccggtagact gcgcagtgtt ctcattggga gacatcactc tcaccggcag
      121 ctccgagatt ctgggcaacg cgggaaccaa taccatagcc aagggtaaag tcaagttcga
      181 atggagcaca gcggtagacg gagatctatc gataggacca agcggcgtcc ccggcaccgt
      241 ccttgacgtc cgcgccggca gtcctatgga ccagtacctg aagtctggca gagtgcagaa
      301 cttgcccgcg gtacgatcct acccaatgcc cgcgtttccg acatatcccg cgcttcccaa
      361 taagggctct ctcacggctg gatggtggcc gagaccgcca tattccatca ccgaagccga
      421 cggagatgcc gttggtggcg atggccgccg aagaggcgac ccgggtcacg gtcttgaagg
      481 ccttggcgag cgcgacggtg ttgttgttcg ggcccgcgcg ggactcgatc aactcctcgc
      541 cgtactcgtc ggtgccgtaa accgtgatgg tcttgcccga gtggtcggtc gtcgccacag
      601 tcagggtgat gttccgggga acgtcgagga tcgccacgcc gtcgaccacc cgggccccgt
      661 cgaggacgaa atcggtggcc gagccgagga gttgaacagc ggcgacgccg tccacatcag
      721 cggtgatcgg agcgccgagg ttgacccgca cgacgccagc gtaagacgcg ttgttgacgc
      781 ccggaggacc gcggtcctgc cggccatcca cgaaggcgcc accgccgcga tccagttgca
      841 gccggaacgc cgagcccgcg ggcagggtgt tggccgcgcg ccaggtgatc gtgatgaccg
      901 tggcagaggt gtaggccacc ccgaagtcgt tcggagagga gtattccttc tgcccgacga
      961 tcagcttgtg ggccttgccg gagcgatagt cgccgatgcg gccgccactg gggtagccgg
     1021 tggtgaaagt gccggacgtc gcaaccgcgg acgccaggac accagacaga gtatcgaagt
     1081 cgttcaactc agtggtcctt cagcttaggc ggcttcgtag acggcgtggc agttcatctt
     1141 gcggcagatg acagtgccgg tccaagtcac gccccggtag aggacgtact gggacggcgg
     1201 ccgggccggc gaatgcttct tcatgtcttc gccgtccatc acgtacggat agaggtggcg
     1261 ggtgtcgatg aagtaggcga agttctcttc gtccaggtcg tccagagtcg ggtcgtactg
     1321 gatcttcccg acaccgcgca tggagatgtc ggccatgccg atctccgtcg cgccggcctt
     1381 caggaagccc tccatggtgt aggtgccctt ctcgtggatc tccgcctcca gcttctcgat
     1441 gaagccggag ccggcgagca cgaggctcgg gcgcccgcca tagcggcgaa gctgccggtt
     1501 ctcggcgcgc aggaacttag tcagcgtctg gttggccgcc gagtgggtga tcttgccggc
     1561 gccagtggcc gcgcggttac gccaccaagg gttctggctg cggtcgatgc cgcccacgat
     1621 gccggtcgtc gggtccttgg cgatgaagtg ctggatgccg gcgaacacct tcgaagactg
     1681 ggtgccgtcg agccacatca tctcgttcat ggagcgggcc atgccctcgt ccatgtcctc
     1741 cagcttgtct tgcaggaggt tggagatcgc gaccttctcg cgctcggagt ggttgctggt
     1801 gctctcgccc gtggtcgagt cggtgacgga gatgccgtca tgcttcagct cggtcagggt
     1861 gacggacagg ccggcgtgca actccttcca ggagtagctg tactgcttga tgttggccgg
     1921 gttgccgtag gtcacggtgt cgtcgtggct gtacccgacg aaggtcgtca ggtagtcggc
     1981 cttgacgttg cctttgatct cgcccttgcc gcccgggaag gtcttctgga cgcccttcat
     2041 ggcgcccatc aggggttttt cctgaatggt ctgagagagg ggctggccct tgaggtagta
     2101 gtcgaggctc gcgttggcga tgttctcgag ctggagtgcg gtgaaaggca cctgtggaga
     2161 tccctggggg ttaggctccc gacgccagtg cgatgttcac gacatcgagc atggacttgg
     2221 ggtcggcagc gacgttgctg gtcgagtggc tccccgggtt ccgcctcacc acctccggct
     2281 tcggaggcag catgcctttc agttggtcgg tgaccgcctt gtgggcctgc tccgctcgcc
     2341 tgaccgcgtc ctccgctgat ttcggagggc cgagcctggc gttgagcgcg atgacgcgat
     2401 ccgcgatgag ctcttccttg gccgcgaaat cggggtccga cgctctcgtc cgctgctccc
     2461 acccgctgac cgcatcgacc acctcaccct ggaagcgctc cacctgggtc gattgctcga
     2521 cgtggcgggc gtgggtggtg gcgaccttcg cggtctcggt cgctcgagcc tcgcgggccc
     2581 ttgcgatcga cagttcccgg gcccgttgct cctccagcct tccttcctcg acgtccttgc
     2641 gaaggtcttc gggcagggcg tgaccggtga tcttgtccag gtcgatcagc cgccggatca
     2701 gctcttggcg agccgcttcg gggttgttct tcaggagcgc gccaacctgc atcagctcca
     2761 cgaactcggc cggctggatg cccgcattgc gcaggaagcc gtcgatccgc tgatactgac
     2821 cggccgggcc ctccagttcc tggacttggg cctggaactc agaagcctgc tgcttgaacg
     2881 cgttgcgctc gcccagcacc ttcttgaacc gggggtgctt accgaacggg agcttggcca
     2941 gttcctcctc ggagagctct tgctcacctt cgggggcctt ggcgtcgtcc gacgtcacgg
     3001 tttccgaggc cgacgtttcc tcgggtgctt ccatcttcgc gggctcgacg acgttcgaga
     3061 tgacgtcgag cagcgacttc ggcggcgctt ctttatcgtc ggagggtggc gatccctcgg
     3121 tgacgccggc tgcgggggac gctcccgcgg aagtctcgtc cagtgaagcc gcgggggctt
     3181 cgtctccgtt ttccgaagcg ggcgaggcct cggaatcatg ctccagcatg cgttacgccc
     3241 ttcgttgtag cctgaagata gtccgaaagc ggaaaatagg caacacctag gccatgacgt
     3301 tcggctgggc ggcgtccggc gccggcagcg cggcctgcgg gccgcccgtg gaggtgtcgt
     3361 cgttcggtgc gttctggccg ccctgttcgc cctgggcgtc ggggctgttg gccggatcct
     3421 cggcgccagg ctgggtcatc cggttcatgc tcacgatggc cggcaggccg gcggtcaccg
     3481 cctcttccca gtcgatcgtg ttgtcaagca gttggatgag gtacttggcg aaccactccg
     3541 ggttgatgcc cgggatctgc accatggtcg gcgcgacccg ttcgaagttg gccaggtcgc
     3601 gttccttgtt ggggcgaccc gaggaaccgg cttggatctc gaggtaaagc tcctcggcga
     3661 tctgctggtt ggtcagcgtc ggccagacgg cgccggggcc gcacacctgc ttgacgatgg
     3721 gctcggacat ctcgcgcagg agcacctggc ccgacagccg cgccaggatc gacaggaagt
     3781 cgtccaggtc gtcgatc
//
LOCUS       DAOQYE010000075         3729 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_25571_length_3729_cov_2.409091, whole genome shotgun sequence.
ACCESSION   DAOQYE010000075 DAOQYE010000000
VERSION     DAOQYE010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 3729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3729
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25571_length_3729_cov_2.409091"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>540
                     /locus_tag="PKN52_02140"
     CDS             <1..>540
                     /locus_tag="PKN52_02140"
                     /inference="COORDINATES: protein motif:HMM:NF013116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HNQ98770.1"
                     /translation="SVASFFLSRIDVMIDPRLDELARQPVPQAHAARQLRGTAAVASA
                     KGAYVIYEQLFGAANERFARLRAAGARPQRLLWASTSTKDPNYPDLKYVEPLVGPDTI
                     NTLPLATLEAYRDHGDPASRIQEGGADARAMLQALSGVGIDLDAVTAKLEEEGVVKFI
                     KAFDDIMTALEGALAQAPAT"
     assembly_gap    541..550
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(572..1345)
                     /locus_tag="PKN52_02145"
     CDS             complement(572..1345)
                     /locus_tag="PKN52_02145"
                     /inference="COORDINATES: protein motif:HMM:NF014193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HNQ98771.1"
                     /translation="MSSRPVLLRGRWLAGHLLALVMVGLFINFGFWQLRRLEQRRAVN
                     AVMDSRAHLPPQPLASALPVGSEVPQYLPVTVVGTFDPEHEVLLRGRSLDGRPGFNLL
                     TPLVLDDDSAGEYRGGAVLIERGWVPYDHDSVPVADALPPTGSVTVSGELRAPQSPPT
                     GPLAAIAAHDPPDGPLVQSFYVDVKRLAPQMPYPLVPAYVVLRQQTPPNEQRLPEPIP
                     ANEMSEGPHLGYALQWFGMALVGAVGYVFLTRSAARQKS"
     gene            complement(1371..2294)
                     /locus_tag="PKN52_02150"
     CDS             complement(1371..2294)
                     /locus_tag="PKN52_02150"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:NF003349.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HNQ98772.1"
                     /translation="MTDLDEHMTNSQERPKATWRDYWILTKPKVISLLLLTAVGAMFI
                     AAGGMPGWLPLLGMLVGGFMSAGAAGVYNMVYDADIDVNMKRTAKRPMVKGSVTPRNA
                     LMFAVALTILSFVVIALTSNVLAALLSWAGIAFYVLIYTMWLKRSTWQNIVIGGAAGA
                     IPPLVGWAAVTNDLSLLAWILFAVVFAWTPVHFWALALMVKKDYANVGVPMAPAVIGD
                     RATVMQMVFYTFLTIVLTILPFFLHQFSLAYFLAALVLNLVLVVRMARVFMVVRAGQT
                     VDRVTALPLYLYSMSYLALLFVVMALDRAIF"
     gene            complement(2291..3259)
                     /locus_tag="PKN52_02155"
     CDS             complement(2291..3259)
                     /locus_tag="PKN52_02155"
                     /inference="COORDINATES: protein motif:HMM:NF014665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HNQ98773.1"
                     /translation="MKGPSTALLAWLALAGNTVVILQGAIVRGTGSGAGCGSHWPTCN
                     GTIVPIDPSLNTVIEFSHRLLSGAVLVLGAWLLVRGLRARKERPGLAAYAALSFGFLV
                     LEALIGAATVLLGLTGDNTSIARGVWVAAHLVNSLLLIGALAGAVVYARRDPPGYPLR
                     LGAQANLAAALAVGLGAMLLVMFTGGIAAMGNTIFPSESLADGFRADFDPNSHLLIRL
                     RVLHPLIAIGAGTYLFVSLGLAWWLKPVPAARRTAQLLLVVYLAQLVIGVANLAFLAP
                     LAVQVLHLSVAVAAYALLSAASIQILGGQQVPSAVPRGALVGSAGR"
BASE COUNT          662 a         1241 c         1309 g          507 t
ORIGIN      
        1 gtccgtcgcg agcttcttcc tgagccgcat cgacgtgatg atagacccgc gcctcgacga
       61 gctcgccagg cagccggtgc cgcaggccca cgccgcgcgc caactgcgcg gcacggccgc
      121 cgttgcgagc gcgaagggcg cgtacgtcat ctacgagcaa ctgttcggcg ccgccaacga
      181 gcgtttcgcc cgcctgcgcg cggccggcgc gcggccgcaa cgcttgctgt gggcctcgac
      241 cagcacgaag gacccgaact acccagacct aaagtacgtc gagcccctcg ttggccccga
      301 cacgatcaac acgctccccc tcgccacgct cgaggcttac cgcgaccacg gcgaccccgc
      361 ctcgcgcatc caggaaggtg gcgccgacgc gcgggccatg ctgcaagcgc tgtcaggcgt
      421 cggcatcgac cttgacgcgg tgacggcgaa gctcgaggag gagggcgtcg tcaagttcat
      481 caaggcgttc gacgacatca tgacggcgct ggagggcgcg ctcgcgcagg cgcccgccac
      541 nnnnnnnnnn ccaccgggcg ctagaaccgt ctcaactctt ctgccgcgcg gcagaacgcg
      601 tcaggaagac gtaaccgacg gcgccgacga gcgccatgcc gaaccattgc agcgcgtagc
      661 cgaggtgcgg cccctcactc atctcgttcg ctgggatcgg ctctggaagc cgctgctcgt
      721 tcgggggcgt ctgttgccgt aggacgacgt aggcggggac gagcgggtac ggcatctgcg
      781 gcgccagccg cttcacatcg acgtagaagc tctgcacgag cgggccgtcg ggcggatcgt
      841 gcgcggcgat ggcggcgagc ggtccggtcg ggggcgactg cggcgccctg agttcgcctg
      901 acacggtaac gctgccggtc ggcggcaacg cgtcggccac cggcaccgag tcgtggtcgt
      961 aaggcaccca gccgcgctcg atcagcacgg caccgccccg gtactcgccg gcggagtcgt
     1021 cgtcgagcac cagcggcgtt aggaggttga agccgggtcg gccgtcgagc gaacgcccgc
     1081 gcagcagcac ctcgtgctcg gggtcgaacg tgccgaccac cgtcacgggg aggtactggg
     1141 gcacttcgct cccgacgggc agcgcgctgg cgagaggctg cggcggcagg tgggcccgcg
     1201 agtccatgac ggcgttcacg gccctgcgct gctcgagacg ccgcagctgc cagaagccga
     1261 agttgatgaa caggccgacc atgacgagcg ccaacaggtg cccggccagc cagcgaccgc
     1321 gcaggaggac cggccgcgag ctcatcgagc gtccactcgg gccacggccc tcagaagatg
     1381 gcgcggtcga gcgccatcac gacgaagagc agcgccaggt agctcatgga gtacaggtag
     1441 aggggcaacg cagtgaccct gtcgacggtc tggccggcgc gaacgaccat gaagacgcgt
     1501 gccatgcgca cgaccaggac gaggttgagg acgagcgccg cgaggaagta agcgaggctg
     1561 aactggtgca ggaagaacgg caggatcgtg agcacgatgg tcaggaaggt gtagaagacc
     1621 atctgcatga ccgtggcgcg gtcgccgatc acggccggcg ccatgggaac gccgacgttg
     1681 gcgtaatcct tcttgaccat cagggccaaa gcccagaagt gcacgggcgt ccaggcgaac
     1741 acgacggcga agaggatcca ggcgagaagg ctcaggtcgt tggtgaccgc cgcccagccg
     1801 acgagcggcg ggatggcgcc ggcagcgccg ccgatgacga tgttctgcca ggtgctccgc
     1861 ttcagccaca tcgtgtagat gaggacgtag aaggcgatgc ccgcccagga gagcagggcg
     1921 gcgagtacgt tgctcgtcag ggcgatgacg acgaacgaca ggatggtcag cgcgacggcg
     1981 aacatcagcg cgttgcgggg cgtgacggag cccttgacca tcggacgctt ggccgtgcgc
     2041 ttcatgttca cgtcgatgtc ggcgtcgtag accatgttgt agacaccggc cgcgccggcc
     2101 gacatgaagc cgccgacgag catgccgagc agcggaagcc agccgggcat gccgccggca
     2161 gctatgaaca tggcgccgac tgccgtgagg agcaggagcg agatgacctt cggcttggtc
     2221 aggatccagt agtcccgcca cgtcgccttc gggcgctcct gggagttcgt catgtgttcg
     2281 tccaagtctg tcatcgtccg gcgctcccca ccaaggcgcc gcgcggcacg gcgctcggca
     2341 cctgttgccc accaaggatc tggatgctcg cggcggacag gagggcatag gccgccacgg
     2401 ccacgctcaa gtgcagtacc tgcacggcca gcggcgcgag gaacgccagg ttggcgaccc
     2461 cgatgaccag ctgcgccagg taaacgacca acagcagttg cgcggtccgc cgggccgcag
     2521 gcacgggttt gagccaccac gccagcccca acgacacgaa caggtaggtg ccggcgccga
     2581 tggcgatgag cgggtgcagc acccgcaggc gtatcaggag gtgcgagttc ggatcgaagt
     2641 cggcgcggaa gccgtccgcc aacgactcgg acgggaagat ggtgttgccc atcgccgcga
     2701 tgccacccgt gaacatcacg aggagcatcg cgccgaggcc gaccgcgagc gcggcggcga
     2761 ggttcgcttg tgcaccgagg cgcaagggat agcccggcgg gtcgcgtctg gcgtagacga
     2821 ccgcgccggc gagcgcgccg atgaggagca gcgagttgac taggtgcgcg gcgacccaga
     2881 cgccgcgtgc gatgctcgtg ttatcgccgg tgagcccgag caggacggtg gccgcgccga
     2941 tcagggcctc caggaccagg aagccgaagg agagcgccgc gtaggccgcc aggcccggtc
     3001 gctccttgcg ggcgcgcaac cccctgacga gcagccaggc gccgagcacc aagacggcgc
     3061 cggagagcag gcggtggctg aactcgatga cggtgttcaa ggacgggtcg atcggcacga
     3121 tggtgccgtt gcatgtgggc caatggctcc cgcacccggc gccggagccc gtgccgcgca
     3181 cgatggcccc ttgcaggatg acgaccgtgt tgcctgctag cgccagccag gccaggagcg
     3241 ctgtcgatgg acccttcacg tgtgaccgct acccgcttcc ttcccgctcg cgccgagatc
     3301 aggacgcggc gaggccgctg acgctcgagg cctcttcatc cataacggtc ggcccttacg
     3361 acaacttgcc catgctatca gaggtgccgg ccccgtgccc ggcgccgcga gccatcgaga
     3421 ccttccgggg ccccaagcgc ccgaccggac ctcgcaggcg ggccgaccac gcgcaccggc
     3481 tgctcgtcga agccggacat gccgcgccac acggcacacg gcgctctcac actatctggc
     3541 gatgatgtct gtcgatgaag gatcggcggg gggttaaaga ccgcttcgct ggtttctacc
     3601 gtctctcggg gcgatgttag actcgccgca ccgaattgag gtcttccgga ggacacgttg
     3661 atcaggacct ggttcacgag cacaacttcg ctcagacacg cgttggccgc gctgctcgtc
     3721 gcggtcgtc
//
LOCUS       DAOQYE010000076         3716 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_25685_length_3716_cov_2.984430, whole genome shotgun sequence.
ACCESSION   DAOQYE010000076 DAOQYE010000000
VERSION     DAOQYE010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 3716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3716
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25685_length_3716_cov_2.984430"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..458
                     /locus_tag="PKN52_02160"
     CDS             <1..458
                     /locus_tag="PKN52_02160"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNQ98774.1"
                     /translation="RPDRPLPSARRGADGPPVATVTGAGGVVFDPGNRVLLLQHASGH
                     WVFPKGHVEQGETQLQAALREVAEEAGVTATCAKPQRTWTTYYRNPRGVQRLITWFRC
                     EADDTTTNVTEEAFVAGGFYAPSEAIELLTHRTDRDLLRRVLNEGERGS"
     gene            455..1615
                     /locus_tag="PKN52_02165"
     CDS             455..1615
                     /locus_tag="PKN52_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98775.1"
                     /translation="MTDSGSNERSARLAQGATPRSEQATLGLTPTAGGRAPDAGPAGV
                     AGAQGMLGDTGEHSAQWLLENLRAIRHSGTLALADGKGTTLLLLARGQVESSFKLGPY
                     ARLDAPEQRFHLHTHEPSVTPQLPPRFPTSQSPFLRALPRLAPPQRLTPGAIDLPDLL
                     ERLHESGFDGSLSYATERERSVALLVGGSVRAAVHEVGGVVHDRAEAMRALQKAGQAR
                     AKGLLELDALEPAVVLPLAALALGRSAPSDGTAFTGLEVFGAGFRFYKQGQPFLEVMA
                     PTEGLTRRYALAEQAVERAPQLELPDEPPGWEDRRFALTLRGRDALNPMTALHMTFRS
                     EHGPDGQRLLEALGKGDTLGRTADALGLELGDMRPWLERLERAGMLREVAGR"
     gene            1707..3146
                     /locus_tag="PKN52_02170"
     CDS             1707..3146
                     /locus_tag="PKN52_02170"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HNQ98776.1"
                     /translation="MSEHPQIERTRIEHTYEVSLRELYLIFRRGFVTIVAVALAFALA
                     AFGYLSTRPATYVASATVQVTAPQPTTTGELASLVPQVGMGVQSYTSLALSNAVLTES
                     LRSGGEAAATDEDQAADPEGLRRLMSSLKLAAIDTSNQSRGQLTVQHTATADSPKGAA
                     DLANAWAQASARAATEAMRGAVDVSVTAAGRELAAREAELTVARDAWTAFSKEDARQA
                     LQSQLAQLAVQQASARSRLAELDILIVSNGARQALLAAAAEARTEASPTPLEAQVRAL
                     VDAGALDAATAAGLAAAISQLPAGVAAGDQSVLALVSRARLESLAADLAGQVAERDQL
                     QNDSDRAEREATALRERLADLELRAATPQAALRNATAAYDRVAAVMPLLTLQQGMVDD
                     AARVIIGASQPLEPKPRNRLTITLAACLVGGLLATLFVFLRAAVAEPATPARPPVLAT
                     GGGNPFSSQVPTDTGRHDDRERRTTSRGR"
     gene            3154..>3716
                     /locus_tag="PKN52_02175"
     CDS             3154..>3716
                     /locus_tag="PKN52_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HNQ98777.1"
                     /translation="MTTSPPSRTGGLLLTGGSGTLGRELLALLPGAFAPTSRDLDITD
                     PDQTTRAITAAAPTVIVHCAAYTDVRAAETERERCWLVNVVGTRNIARAAREAGARLV
                     HVSTDYVFFGDRGGYREDDVPGPVRNYYSLTKLVAEEAVRTLPGSLVVRTSFRPRRWP
                     YPTAFDDLFTSQDYVDVIAPLLAELIA"
BASE COUNT          537 a         1398 c         1290 g          491 t
ORIGIN      
        1 gccgccccga caggccgctc cctagcgcca ggcgcggcgc cgacggcccc cccgtcgcca
       61 cggtgaccgg cgccggcggc gtggtgttcg accccggcaa ccgcgtcctg ctccttcagc
      121 acgccagcgg ccactgggtg ttccccaagg gccacgtcga gcagggcgag acgcagctcc
      181 aggccgcgct ccgcgaggtg gcggaggagg ccggcgtgac cgccacctgc gccaagccgc
      241 agcgcacctg gacgacctac taccgcaacc cgaggggtgt gcagcgcctc atcacctggt
      301 tccgttgcga ggcggacgac acgaccacca acgtgacgga ggaggcgttc gtcgcggggg
      361 gcttctacgc tccatcagag gccatcgagc tcctgacgca ccgtactgac cgcgacctcc
      421 tgaggcgtgt cctgaacgag ggggagcggg gctcgtgacg gattcgggca gcaacgagcg
      481 cagcgctcgg ttggctcaag gggcgacgcc ccggagcgag caggcgacct tggggctgac
      541 gccaacggcc ggcggccgcg cgcccgacgc cggcccggcc ggagtggccg gggcgcaagg
      601 gatgctcggc gacacgggcg agcacagcgc ccagtggctg ctcgagaacc tgcgtgccat
      661 ccggcactcc ggcacgctgg ctttggccga cggcaagggc acgacgcttc tcctcctcgc
      721 ccgcgggcag gtcgagtcga gcttcaagct cgggccctac gcgcggctcg acgcgcccga
      781 acaacggttc cacctccaca cgcacgagcc gtccgtcacc ccgcagctgc cgccacgctt
      841 cccgaccagc cagtcgccct tcctgcgcgc gctgccgcgg ctcgcgccgc cccagcgcct
      901 gacccccggc gccatcgacc tccccgacct gttggagcgc ctccacgagt cgggcttcga
      961 cggctcgctg agctacgcca ccgagcgcga acgctccgtc gccctcctcg tcggcgggag
     1021 cgtgagggcg gccgtgcatg aggtcggcgg agtcgtccac gaccgggccg aggccatgcg
     1081 cgcactgcag aaggcggggc aggcgcgcgc caaggggctc ctggagctcg acgccctcga
     1141 gcccgccgtc gtgctcccgc tcgccgccct cgcgctcggc cgctcggcac cctccgacgg
     1201 caccgcgttc accggactag aggtcttcgg cgctggtttc cgtttctaca agcagggcca
     1261 gccgttcctg gaggtcatgg cgccgaccga aggtctgacg cgccgctacg ctctggcgga
     1321 gcaggccgtc gagcgcgcgc cgcaactcga actccccgat gagcccccgg gctgggagga
     1381 ccgccggttc gccctcacgc tgcgcggtcg cgacgcgctg aaccccatga cggcccttca
     1441 catgaccttc cgctccgagc acgggcccga cggtcaacgg ctcctcgagg cgctcggcaa
     1501 gggcgatacc cttggccgca ccgccgacgc tctcggtctg gagttgggcg acatgcggcc
     1561 gtggttggag cggttggagc gcgcgggcat gctgcgcgag gtagcggggc ggtaggttct
     1621 cgcgccgatg tcaatccgtc agagcctcgt gccgctcccc gacgtatcct aggcgggcgt
     1681 cgaaagcacc ccaggagtca gcaaccgtgt cagagcaccc ccagatagag cgcactcgga
     1741 tagagcacac ctacgaggtc tcgctccgcg agctctacct gatcttccgt cgcggcttcg
     1801 tgaccatcgt cgcggtcgcc ctagccttcg cgttagccgc cttcggctac ctatcgacgc
     1861 gtcctgcgac gtacgtggcc agcgccacgg tgcaggtaac ggccccgcag ccgaccacca
     1921 cgggagaact cgcgagcctg gtgccccagg tcggcatggg cgtgcagtcg tatacgtcgc
     1981 tggcgctgtc gaacgcggtg ttgacggagt ctctgaggag cggcggcgag gccgcggcaa
     2041 ccgatgagga ccaggcggct gaccctgaag ggctgcggcg tctcatgtcg agcctcaagc
     2101 tcgccgcgat cgacacgtcg aaccagtcgc gcggccagct caccgtccag cacacggcca
     2161 ccgccgacag cccgaagggg gcggcggacc tcgccaacgc ctgggcccag gccagcgcgc
     2221 gggcggcgac ggaggcgatg cgcggcgccg tcgacgtctc cgtgacggcg gccggtcgag
     2281 aactggccgc gcgggaagcc gagcttacgg tcgcccggga cgcctggacg gccttctcca
     2341 aggaggacgc ccggcaggcc ctgcagagcc agctggcgca gctggccgtc cagcaggcgt
     2401 ccgcgcgctc gcgcctcgcc gagttggaca tcctgatcgt gtcgaacggg gccaggcagg
     2461 cccttctcgc ggccgccgcg gaggcgcgca cggaagcgtc gcccacgccg ttggaggccc
     2521 aggtcagggc gttggtcgac gccggcgccc tcgacgccgc cacggcggcc ggactcgcgg
     2581 ctgcgatctc ccaactcccg gcgggcgtag ccgccggcga ccagagcgtt ctcgcgctcg
     2641 tcagccgcgc ccgcctcgaa tcgctggcgg ccgacctggc cgggcaggtg gccgagcgcg
     2701 atcagctaca gaacgactcc gacagggccg agagggaagc cacggcgttg cgcgagcgtc
     2761 tggccgacct ggagctgcgg gcggcgaccc cccaggcggc cctcaggaac gcgacggccg
     2821 cgtacgaccg cgtggctgcg gtgatgccgc tgctcaccct ccaacagggc atggtggacg
     2881 acgccgcacg cgttatcatc ggcgcctccc agccgctgga acccaagcca aggaaccgtc
     2941 tgaccatcac cctggcagcc tgcctggtcg gcgggctgct cgccacgctc ttcgtgttcc
     3001 tgcgtgccgc cgtggcggag ccggccacgc cggcgcgccc gcccgtcctg gccacgggcg
     3061 gcggcaaccc tttcagtagc caggtcccta ccgacacagg ccgtcacgac gaccgggagc
     3121 gccgaaccac gagccggggt cgttaggacc ggtatgacca cgtcgccgcc ctcgaggacc
     3181 ggcggcctgc tgctgaccgg cgggagcggc accctcgggc gcgagctgct cgccctcctg
     3241 cccggggcgt tcgccccgac ctcgcgtgac ctcgacatca ccgatcctga ccagacgact
     3301 cgcgcgataa ccgccgcggc gccgaccgtc atcgtccact gcgccgccta caccgacgtc
     3361 cgtgcagcgg aaacggagcg cgagcggtgc tggctggtca acgtggtggg cacgcgcaac
     3421 atcgccagag ccgcgcggga ggccggggcg cgcttggtgc acgtctcgac cgactacgtg
     3481 ttcttcgggg accgcggagg ctaccgcgag gacgacgtgc ctggacccgt gcgcaactac
     3541 tactcgctga ccaagctcgt ggcggaggag gccgtgcgga ccctgccggg ctcgctcgtg
     3601 gtgcgcacga gcttccggcc gcgccggtgg ccttacccaa cggccttcga cgacctcttc
     3661 acgtcccagg actacgtcga cgtcatcgcg ccgctgctgg cggagttgat cgcgca
//
LOCUS       DAOQYE010000077         3693 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_25917_length_3693_cov_3.576141, whole genome shotgun sequence.
ACCESSION   DAOQYE010000077 DAOQYE010000000
VERSION     DAOQYE010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 3693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3693
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25917_length_3693_cov_3.576141"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1247)
                     /locus_tag="PKN52_02180"
     CDS             complement(<1..1247)
                     /locus_tag="PKN52_02180"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNQ98778.1"
                     /translation="MSVLILLLLTLANGVLAMAEIAVVSANKLRLQQKAESGDANAKR
                     ALALSENPNTFLSTVQVGVTLVTVFSGAFGGAALSGPLAEVLARVSWLRDIAQPVAFG
                     IVVVLISYFSIVVGELVPKRIGLNDPEAMAMRLAGVMGFLAALTRPLVLFLGASTNLL
                     LRLLRVKQSGGEEISEEDIDMVIAQGLRAGVIEPAEQEIIENAFWLGERKVNAIMTPR
                     PEVAWLDVGANVRDVLEQVREEPHGRYLVCDGDLDKVVGFVAVRDLFAAGEASWAELD
                     LRAVVRKPLMVPETLPILGLLNNFRESRTHFAVVLDEYGGLEGIVTLSDILEELVGDI
                     LPPGEEEVPEVVELGEGRWRMQGSLHVDDLIDELGLAEEVVPEGAGYQTLGGLLSDRL
                     GRVPQVADETVWNGFRFVVEEMDG"
     gene            1417..2508
                     /locus_tag="PKN52_02185"
     CDS             1417..2508
                     /locus_tag="PKN52_02185"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HNQ98779.1"
                     /translation="MPARRSPTLVVTLLTLLTFTLVAGANAQEADQLQRTAQALFAPL
                     PDVAANPDIDLTDAKIELGKVLYYEPRLSLSGVFSCNSCHNIATYGVDNLETATGHGW
                     AQGPRNSPTVMNAALQASQFWDGRAKDVEEQAGGPILNPIEMGIPSQEFAIERLSSIP
                     QYAELFAKAFPDQAEPLVYANVANAIGAFERTLVTPARFDTYLAGDANALSDLEKTGL
                     QTFISYGCAGCHSGATVGGNSLQRFGVVESYWEATREFVTIDTPTMGMDVGRFAVTHE
                     TQDLYIFKVPTLRNITRTYPYFHDGSVWDLRDATQIMARVQLGRELTEEDLDALMAFY
                     DTLEGEIPAYALQLPVLPASTTGTPKPQH"
     gene            complement(2774..>3693)
                     /locus_tag="PKN52_02190"
     CDS             complement(2774..>3693)
                     /locus_tag="PKN52_02190"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNQ98780.1"
                     /translation="DLHAEIAVAAAANGKHIFCEKPIAIDSAEARVMLSAAQAAGVTH
                     YLNHNYRRVPAVALAKRLIDEGFVGQVYHWRSAYLQDWIVDPAFPLTWHLRKKNAGSG
                     PHHDLNSHLIDLARYLVGEVRTVSATSATFVRERPLPGAGAATFSAGSGGGGQLGQVD
                     VDDATFLVAEFENGALGSFDVSRFAPGRKNHNTFEIYGASGSIVFDLERMNELQVFSR
                     RDPSHAQGFRTIIATEAEHPYVSAWWPPGHLIGYEHSFHHAVADFVTAVAHRERVRPD
                     FEDGLREAKVIDAALASAASGERVTVDYD"
BASE COUNT          611 a         1292 c         1203 g          587 t
ORIGIN      
        1 ccgtccatct cctccaccac gaagcggaag ccgttccaaa ccgtctcgtc cgcgacctgc
       61 ggcacgcggc cgaggcgatc ggagagtagg ccgccgaggg tctggtagcc ggcgccttcc
      121 ggcacgacct cctcggcgag gccaagctcg tcgatgaggt cgtccacgtg gagggagccc
      181 tgcatccgcc accggccctc gcccaactcc acgacctccg gtacttcctc ctcgcctggc
      241 gggaggatgt cgccgacgag ttcctcgagg atgtctgaga gggtgacgat gccctcgagg
      301 ccgccgtact cgtccagcac gaccgcgaag tgcgtccgcg actccctgaa gttgttcagc
      361 aggccgagga tggggagcgt ctcgggcacc atcagcggct tgcgcacgac ggcccggagg
      421 tcgagttcgg cccagctagc ctcgccggcg gcgaagaggt ccctgacggc cacgaagccc
      481 acgaccttgt cgaggtcgcc atcgcacacg aggtagcgtc cgtgcggctc ttcccgcacc
      541 tgttcgagga cgtcgcgcac gttcgccccc acgtctagcc aggcgacctc cggacgcggc
      601 gtcatgatgg cattgacctt ccgctcgccc agccagaacg cgttctcgat gatctcctgc
      661 tcggccggtt cgatcacgcc cgcgcgcagg ccttgggcga tcaccatgtc gatgtcttcc
      721 tcgctgatct cctcgccgcc gctctgcttc acgcgtagga ggcggaggag gaggttggtc
      781 gaggcgccga ggaagagcac gagcgggcgc gtgagcgccg cgaggaagcc catcacgccc
      841 gcgagccgca tggccatggc ctcggggtcg ttgaggccga tgcgcttggg aacgagctcc
      901 cccaccacga tcgagaagta cgagatcagc acgacgacga tcccgaacgc caccggttgg
      961 gctatgtcgc gcagccatga cacgcgcgcc aggacctcgg cgagcggccc cgacagcgcg
     1021 gcgccgccga aggcgcccga gaagaccgtc acgagcgtga cgccgacctg aacggttgat
     1081 aggaacgtgt tggggttctc cgagagggcg agggcgcgct tggcgttggc gtcgccggac
     1141 tcggccttct gctggagcct gagcttattg gcggagacca cggcgatctc ggccatggcg
     1201 agcaccccgt tggcgagggt cagtaggagg agtatcagca cgctcacgct tgcgcaacta
     1261 tacgcagtcg ccggcgggag tcgagaacgg tgaccacccc ttggagcgac ctcctggtat
     1321 tggcgcggtc gggacagttg tccattcctc ggaatgattc cgattgttac gctcgaaccg
     1381 tcgcggcccg atcgccgctt gagaaaggat gcccctatgc cggcgaggag atcacccact
     1441 cttgtcgtca ccctgctcac actcctgacg ttcaccctgg tggccggcgc caacgcgcag
     1501 gaggccgatc agctccagag gacggcgcag gccctcttcg cgccgctgcc ggacgtcgcg
     1561 gccaacccgg acatcgacct gacggacgcc aagatcgagc taggcaaggt gctctactac
     1621 gagccccgcc tctcgctctc cggcgtcttc tcctgcaaca gttgccacaa catcgcgacc
     1681 tacggcgtcg acaacctcga gaccgccacc ggccacggct gggcgcaagg cccgcgcaac
     1741 tcgcctaccg tcatgaacgc ggcacttcag gcttcccagt tctgggacgg ccgcgccaag
     1801 gatgtcgagg agcaggccgg cggccccatc ctcaacccga tcgagatggg catcccatcg
     1861 caggagttcg cgatagagcg cctctcctcc atcccgcagt acgcggagct cttcgccaag
     1921 gcgttccccg accaggccga gccgctcgtc tacgccaacg tcgccaacgc catcggcgcc
     1981 ttcgagcgca cgctcgtcac ccccgcgcgc ttcgacactt acctcgctgg cgacgcgaac
     2041 gccctgagcg acctcgagaa gaccggcctg cagaccttca tcagctacgg ctgcgccggc
     2101 tgccacagcg gcgcgacggt cggcggcaac tcgctgcagc gcttcggcgt cgtggagtcc
     2161 tactgggagg ccacccgcga gttcgtgacc atcgacacgc cgacgatggg catggatgtc
     2221 gggcgcttcg ccgtcaccca cgagacgcaa gacctctaca tcttcaaggt cccgacccta
     2281 cgcaacatca cgcgcaccta cccgtacttc cacgacggct ccgtgtggga cctgcgcgac
     2341 gccacgcaga tcatggcccg cgtccagctc ggccgagagc tgaccgagga agacctcgac
     2401 gccctgatgg ccttctacga cacgctcgag ggcgagatcc ccgcatacgc gctgcaactg
     2461 cccgtgctgc ccgccagcac gacggggacg cccaagccgc agcactgagc ccatagcgct
     2521 gagcctcgca gctctgggtc agcgccagcg gggcgcgccg gatgatcggc gcgccccgga
     2581 tactcagcgg ggaccgcaaa ccacctcggg cgatcgtgtc cttggcggtc ggaggtcttc
     2641 actagcgcca cacgcatacg ctctcgcggt cgatggtcat ccatccaccg ctgcgcgagg
     2701 ccgccgccgg caacgaggcg gcgccactcc ccttaggatc ttgcgcctca cgtcctttgg
     2761 tcaggctaga tccttagtcg taatcgacgg tcacgcgctc cccgctagcc gccgacgcca
     2821 gggcagcatc gatgactttg gcctcgcgca ggccatcctc gaagtcgggg cggacccgct
     2881 cccggtgggc cacggccgtc acgaagtcgg ccacggcgtg gtggaaggag tgctcgtagc
     2941 cgatgaggtg acccggcggc caccacgcgc tcacgtaagg gtgctcggcc tcggtcgcga
     3001 tgatcgtcct gaacccctgc gcgtggctcg ggtcgcgcct ggagaagacc tgcagctcgt
     3061 tcatgcgttc gaggtcgaac acgatgcttc cgcttgcacc gtagatctcg aaggtgttgt
     3121 ggttcttgcg gccgggggcg aagcgcgaga cgtcgaacga ccctagggcg ccgttctcga
     3181 actcggcgac caggaaggtg gcgtcgtcga cgtcgacctg accgagttga ccaccgccgc
     3241 cgctcccggc agagaaagtg gcggcgccag cgccgggtag tggtcgctcc cgaacgaagg
     3301 tggcgctggt ggcgctcacc gtcctcacct cgccgaccag gtagcgcgcg aggtcgatga
     3361 ggtggctgtt gaggtcatgg tgcgggccgg agccggcgtt cttcttgcgg agatgccacg
     3421 tgagcgggaa cgccgggtcg acgatccagt cctgtagata agcgctgcgc cagtgataga
     3481 cctgcccgac gaagccctcg tcgatgaggc gcttggccag cgcaacggcc ggtacgcgcc
     3541 tgtagttgtg gttcagatag tgggtcacgc ctgccgcctg cgccgccgag agcatgaccc
     3601 gcgcctcggc gctgtcgatg gcgatgggct tctcgcagaa gatgtgcttg ccgttggcgg
     3661 cggcggccac ggcgatctcg gcgtggaggt cgg
//
LOCUS       DAOQYE010000078         3676 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_26101_length_3676_cov_2.450428, whole genome shotgun sequence.
ACCESSION   DAOQYE010000078 DAOQYE010000000
VERSION     DAOQYE010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 3676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3676
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26101_length_3676_cov_2.450428"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            62..352
                     /locus_tag="PKN52_02195"
     CDS             62..352
                     /locus_tag="PKN52_02195"
                     /inference="COORDINATES: protein motif:HMM:TIGR02607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HNQ98781.1"
                     /translation="MFDHPHPGEILKEDVLEPLGLSVTEAAERLGITRPNLSRVLNGK
                     AGISPELALRLELAGASTARFWMNLQTNYELTEAAKRPRPRTKPLTNSLVAH"
     gene            428..3418
                     /locus_tag="PKN52_02200"
     CDS             428..3418
                     /locus_tag="PKN52_02200"
                     /inference="COORDINATES: protein motif:HMM:NF024767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNQ98782.1"
                     /translation="MPPFCGREDHLATLLSRWQEVKQGVGPRVVVLLAESGLGKTRLA
                     QEFYARLVQTEQAEGGYWPAELGTDGNNLLVNPPPAGWNAAAEMPFLWWGVRLSDPVG
                     RNQVATGALAAHVDGYLVPHLEPFHREQRRRQRLGQLAKVGGAVAVDAVLDLVPVLNL
                     IKKAGEVGLELKGIHDSWRKDRQALDAAALLGQRRDSLVDQLLGDLGKLFDGPSGRTV
                     PAVILVDDAQFSNVDPGVTAFVQALLKAMAAGDWPLLLLVTHWEREFAEAADGEVDAS
                     VAAIIARQARLRPERVEMLRLAPIAGLEPLVTERLPGLPAAQVARLTERAGGNPQYLD
                     EIVRLTLDPRSRAWFEGRDPTGALTNAGLETLLAKSVKLQDVVAERFANSPAEVQEAV
                     ALAGVQGAEFLEALVELTHRRLLVDQAGDQAGGASAGQAGGRADGRTGGPAAGDAVAH
                     ALADACQRHGYVAWLSEERGAFSQRIYQDVAREFLPVFHDEAAAEDALRAVVAQVMHG
                     ELPLPVTADAERALMRLAADLFETSDDEEERRLAAHGLHRLTLAASTAGELQVAHGMA
                     VRQSALLEGISDARQDGDLAWLRLTNDVLATAADTEARRSVLTRLVRLTGEAYEDDVN
                     TWSAALYAQVLMDVAEFHEEAGAQELRSEAINAAVSVVQSLDGFEPEVEVLEASLRLH
                     RMYAAWFDEYGRLDDALETYRYALGVANQLLALEDDPVRRLQVATVQSEVGTNALLRG
                     DARGALPDLEQAAATLRALAEGAASVDLDIRLSTALDHLADAYSATGRPRDAEALLVE
                     VLELMRRHLNMAPDAPRTMANVADALERLTVLQQRLGAGAGAAQHIREAVALRRTVAE
                     RSRAVPDYAMLGYSLTRAGEIVGSQGAVAEGHEYVKEAVNLSRRVCASDTSARTAWRL
                     LLALKVALDYALHHTDLEAARELLAEVDAVKGRLEPDALTPITPYLRDIEEQRARVLE
                     ESGDAEGAARVRAGGRVGEGGAVN"
BASE COUNT          574 a         1310 c         1296 g          496 t
ORIGIN      
        1 tcgctggaac cgacgttgat cgcgtcgact acgtcgacta tcactgaagg agctgactac
       61 aatgttcgac caccctcatc ccggcgagat cctcaaggaa gacgtgcttg agcccctggg
      121 tcttagcgtc accgaagcag ccgagcggct cggcatcacg cgtcccaacc tctcccgcgt
      181 actgaacggc aaggccggca tcagccccga gttggcactt aggctcgagc tcgccggcgc
      241 cagcaccgca aggttctgga tgaacctgca gacgaactac gaactcactg aagccgccaa
      301 gcggccgcgg ccgcggacca agcctctaac gaactctctg gtagcgcatt agtctccagc
      361 aagctcgtcc agcaaggtcc tcgtagacac cgccccgccc caccgcaccg tctggtactt
      421 ttctcccatg cccccgttct gcggccgcga agaccacctg gccaccctgc tgtcccggtg
      481 gcaggaggtc aagcaaggcg tcgggccgcg cgttgtcgtg ctgctggccg agtctggcct
      541 cggcaagacc cggctggcgc aggagttcta cgcgcgccta gtccaaaccg agcaggcaga
      601 aggtggctac tggcctgcgg aactgggcac cgacggcaac aacctgctcg tcaacccgcc
      661 cccggccggc tggaacgccg ccgccgagat gccgttcctg tggtggggcg tgcgcctgtc
      721 cgacccggtg ggccgcaacc aggtggccac cggggcgctg gccgcgcacg tcgacggtta
      781 cctcgtcccg cacttggagc cgttccaccg cgagcagcgc cgccggcagc ggctcgggca
      841 gctggccaag gtgggcgggg ccgtcgccgt cgacgccgtc ctcgacctcg tccccgtgct
      901 caacctgatc aagaaggccg gcgaggtcgg gctcgagctc aagggcatcc acgattcgtg
      961 gcgcaaggac cgccaggcgc tcgacgccgc cgcgctgctc ggccagcggc gcgactcgct
     1021 ggtggaccaa ctgttgggcg acctcgggaa gctgttcgac ggcccgagcg gccgcacggt
     1081 gccagcggtc atactcgtcg acgacgcgca gttctcgaac gtcgatccgg gcgtcaccgc
     1141 gttcgtgcag gccctgctca aggccatggc ggcaggcgac tggccgctgc tcttgctggt
     1201 gacgcactgg gaacgcgagt tcgccgaagc ggccgatggc gaggtcgacg cgtccgtggc
     1261 cgccatcatc gcccgccagg cccgactacg gcccgagcgg gtcgagatgc tgaggctcgc
     1321 gcccatcgcc gggctggagc cgctcgtcac cgaacgcctg cccggcctgc cggctgcgca
     1381 ggtggcccgc ctgacggagc gcgccggcgg caacccgcag tacctcgacg agatcgtgcg
     1441 cctgacgctc gacccgcgca gccgcgcctg gttcgagggg cgcgacccga ctggcgccct
     1501 gaccaacgcc gggctcgaga cgctgctcgc caagagcgtg aagctgcagg atgtcgtggc
     1561 cgagcgcttc gccaacagcc ccgccgaggt ccaggaggcc gtggcgctgg cgggcgtgca
     1621 gggcgcggag ttccttgagg cgctggtgga gctgacgcac cggcggctgt tggtcgacca
     1681 ggcgggcgac caggcgggcg gcgcgagcgc tggtcaagcg ggcggtcggg ccgacggccg
     1741 aacgggcggt cctgccgccg gcgacgccgt cgcccacgcg ctggccgacg cctgccagcg
     1801 ccacggttac gtcgcctggc tcagcgagga gcggggtgcg ttcagccagc gcatctacca
     1861 ggacgtggcc cgcgagttcc tccccgtgtt ccacgacgag gcggccgccg aggacgcgct
     1921 acgcgcggtc gtcgcgcagg tgatgcacgg cgagctgccc ctccccgtga ccgcggacgc
     1981 ggaacgcgca ctgatgcgcc tcgccgccga tctgttcgag acctcggacg acgaagagga
     2041 gcgccgcctc gccgcccacg ggctgcaccg gctcaccctc gccgcctcga cggcgggcga
     2101 gctgcaagtc gcccacggga tggccgtgcg gcagtcggcg ctcctggagg gcatctccga
     2161 cgcgcgtcag gacggcgacc tggcgtggct gcgcctcacc aacgacgtac tggcgaccgc
     2221 cgcagacacg gaggcgcgcc gctccgtgct cacgcgcctg gtccgcctca ccggcgaggc
     2281 gtacgaggac gacgtgaaca cctggtcggc cgccctgtac gcgcaggtcc tcatggacgt
     2341 ggccgagttc cacgaggagg ccggcgccca ggagctgcgc tcggaggcga tcaacgccgc
     2401 ggtgtccgtg gtgcagagcc tcgacggctt cgagcccgaa gtcgaggtgc tggaggcctc
     2461 gctgcgcctc caccgcatgt acgccgcgtg gttcgacgag tacggcaggc tcgacgacgc
     2521 cctggagacc taccgctacg cgctcggcgt cgcgaaccag ctcctcgcgc tggaggacga
     2581 ccccgtgcgg cgcctgcagg tcgccaccgt gcagagcgag gtgggcacta acgcgctctt
     2641 gcgcggcgac gcccggggcg ccctccccga cctggagcag gccgccgcta ccttgcgcgc
     2701 actggccgag ggcgcggcga gcgtcgacct cgacatccgc ctgtcgaccg ccctcgacca
     2761 cctggcggac gcgtactcgg ccacggggcg cccgcgagac gcggaagcgc tgctggtgga
     2821 ggtcctcgag ctgatgcgcc ggcacctgaa catggcaccc gacgcaccca ggaccatggc
     2881 caacgtagcg gacgcgctgg agcgactgac cgtcctgcaa cagcggttgg gggcgggcgc
     2941 gggcgcggcg cagcacatcc gggaggccgt ggccctgcgg cgcaccgtgg cggagcggtc
     3001 gcgcgcggtg cctgactacg ccatgcttgg ttactcactg acccgcgcgg gcgagatagt
     3061 gggctcgcaa ggcgcggtgg ccgagggtca cgagtacgtg aaggaagccg tgaacctgtc
     3121 gcggcgcgtg tgcgcgagcg acacgagcgc ccgcacggcg tggcgcctgc tcctcgcgtt
     3181 gaaagtggcc ctcgattacg cgctacacca cactgaccta gaggccgcgc gcgagctgct
     3241 ggcggaggtc gacgcggtca aggggcgcct cgagcccgac gcactgacgc ccatcacgcc
     3301 gtacctgcgc gacatcgagg aacaacgcgc gcgcgtgctg gaggagagcg gcgatgccga
     3361 gggcgccgcg cgcgtgaggg ccggtgggcg ggtcggggag ggtggggcgg tgaactgagg
     3421 tgatagccgc cgttaaggcg cgcgcaggtt catgtcgtcc aacgaccgac caagaaaggg
     3481 cggtcgacga ccgggttgcc ctcctcacct agtacgtgca tgggatcgtg caacaggaga
     3541 ctggcctcct cgtgtatatg cgaagcgcct aggctagcct gacgtcatct tcccggacag
     3601 gctcccacca cgtgaccacc aaggccccaa gcacaaaacc gctgacagtc ccagccagga
     3661 acgactcggt aggagc
//
LOCUS       DAOQYE010000079         3634 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_26538_length_3634_cov_2.526125, whole genome shotgun sequence.
ACCESSION   DAOQYE010000079 DAOQYE010000000
VERSION     DAOQYE010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 3634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3634
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26538_length_3634_cov_2.526125"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            166..999
                     /locus_tag="PKN52_02205"
     CDS             166..999
                     /locus_tag="PKN52_02205"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ98783.1"
                     /translation="MFRGTLRALRRNPLAACGLVWLIALVILVLGQPYLGLPSPTKLS
                     LSNQFQPPSKQHILGTDESGRDVFSRLLSGGRVSLAVGFAGALLTVLIASVLGLAAGY
                     FGGTIDEILMRLTDTLMAIPTFFLIMVIVAIWGSGVVVLVLALALTRWMDVARLVRAE
                     VLRTKDLEYVSAATALGGSHWRVMFRHLLPQALPSLIVATSLNVGYVMLVEAALSFLG
                     LGVLPPTPSWGNMLSDSQYYVWSAPQLAVYPGVMITLTVLAFNSLGDVLRDLVDPRLS
                     S"
     gene            1062..2129
                     /locus_tag="PKN52_02210"
     CDS             1062..2129
                     /locus_tag="PKN52_02210"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HNQ98784.1"
                     /translation="MAGYPHTTLDDVLPHPLQKGHHTPGTTPYIFIDSCVQIWEDTEY
                     GKLSDYSADAYCITTFRPHDDAGNALDAIADWYKIERSYPNIRIVRTAQDIIDAKANG
                     QTAIIINSQGGDFLCSNMERLDVFQAMGLRMMLPAYNNRNAICDGCLEPTDAGLSTMG
                     REWVAGCNRLGIVMDLTHVGARATFEVMELTTQPVVFSHSNPTGLVKNARNVTDEQIK
                     ACAATGGVVAPTNWGPLNLKAGSTTRPTVNDYLDAVDYIVDLVGIDHVGIGTDMSHGT
                     YPDGDKIRGLPSKRSVGGAYADHIERAPRSRLRYVEGFDDYGQLPTVVELMKKRGYSE
                     EDVGKVLGGNYLRVFRAVWGA"
     gene            2126..3118
                     /locus_tag="PKN52_02215"
     CDS             2126..3118
                     /locus_tag="PKN52_02215"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HNQ98785.1"
                     /translation="MNENARRGRATTHTLLLSQRIGAETEEVLAERFPGLRIVRLPAS
                     GDVPEDARDATVLYRAGMPHEALRTALIQLPHLDWIHTASAGFNWVLIPEVIARPIRL
                     TRTARVLDVPIAEYVVGTTLALLKGLPSLLEAQRARRWEREVDARGLLGATVGVIGAG
                     AIGSAVATRFRPFGTKVIGMKRDPAPLAEFDEILAPGELDRLLTDSDVVVVACPLTPE
                     TRHLLGAREFALMRSHAVLVNIARGEVLVEAELVEALRERRFAAAALDVFTEEPLAAA
                     SPLWDLDNVILTPHISYIDPTNSLRGVVEFADNLERYLAGAPLLNEIKSRDLGY"
     gene            3155..>3634
                     /locus_tag="PKN52_02220"
     CDS             3155..>3634
                     /locus_tag="PKN52_02220"
                     /inference="COORDINATES: protein motif:HMM:NF016817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HNQ98786.1"
                     /translation="MPTFEGLDSLPPAPGRYHVHMPVARDLSGRNVGLDVIVHQGAEP
                     GPTLTVLSGLHGDEWLHLEFFGRLDSEIDHAALRGRVLLVPVANAAAFANLARNVLHE
                     ADAPDMNRVFPVTPRPQNGLPEQLAAKLAHEVLARSDALLDFHLGIWGSTLASSIVGT
                     "
BASE COUNT          607 a         1274 c         1128 g          625 t
ORIGIN      
        1 cgccctcagg cgcgacacgc cgctcatcct cggcgtcacc ctactcgtct cggccgcggt
       61 cgtcgccagc aacctgctgg tagacctcct gtaccctgtc atcgacccca ggatccgcta
      121 ctcatgacta cgagcagcgt cgcgaagggc tctaccaaac gaggcgtgtt cagaggaacc
      181 ctcagggccc ttaggcgtaa cccgcttgcg gcatgcggcc tggtgtggct gatcgccctc
      241 gtcatcctgg tcctcggcca gccctacctc gggctgccct cgccgaccaa gctgtcgctc
      301 tccaaccagt tccaaccccc gtccaagcaa cacatcctgg gcacggacga gagcgggcgc
      361 gacgtcttct cgcggctcct atccggtggc cgcgtatcgc tggcggtcgg tttcgccggt
      421 gcgctgctga cggtcctcat cgcatccgtc ttgggcctgg cggccggtta cttcgggggg
      481 accatcgacg agatcctgat gcgcttgact gacaccctca tggcgatacc cacgttcttc
      541 ctcatcatgg tgatcgtcgc catatggggc tcgggcgtgg tcgtcctggt gctggccttg
      601 gccctaacca ggtggatgga cgtggcccgc ctcgtccgcg cggaggtgct tcgcaccaag
      661 gacctggagt acgtgtctgc ggccaccgcc ctcggtggct cccactggcg cgtgatgttc
      721 aggcatctgc tgccgcaggc gctgccatcg ctgatcgtgg ccacctcgct gaacgtcggc
      781 tacgtgatgc tcgtcgaggc cgcgctctcg ttccttggtc tcggcgtcct gccgcccacc
      841 ccgagttggg gcaacatgct cagcgacagc cagtactacg tctggtccgc cccccaactg
      901 gcggtgtacc cgggcgtgat gattaccctg acagtcctgg cgttcaactc gctaggtgat
      961 gtcctgcgcg acctggtcga cccgaggctt tcctcgtaga tggccgcgcg tagcgacagc
     1021 ccctctcccc acggtttccc tagcaagaga aggagttcga catggctggc taccctcaca
     1081 cgaccctcga cgacgtgctg ccccaccccc tgcagaaggg tcaccacacg ccgggtacga
     1141 ccccatacat cttcatcgac agctgcgtcc agatctggga ggacaccgag tacgggaagc
     1201 tgagcgacta ctcggccgac gcgtactgca taactacctt cagaccgcat gacgacgccg
     1261 gcaacgcgct tgacgcgatc gccgactggt acaagatcga gcgcagctac cccaacatcc
     1321 gcatcgtgcg cactgcccag gacatcatcg acgcgaaggc caacggccag accgcgataa
     1381 tcatcaactc ccaaggcggc gatttcctgt gtagcaacat ggaacggctc gatgtcttcc
     1441 aagccatggg tctgaggatg atgttgcccg cgtacaacaa ccgcaacgcc atctgcgacg
     1501 gctgcctcga gccgacggac gccgggctct cgacgatggg acgcgaatgg gtggccggct
     1561 gcaacagact gggcatcgtc atggacctga cgcacgtcgg ggcacgcgcg acgttcgaag
     1621 tgatggagct caccacgcag ccggtggtgt tctcgcactc gaacccgacg gggctcgtca
     1681 agaacgcccg caacgtcacg gacgagcaga tcaaggcatg cgccgctacc ggcggcgttg
     1741 tcgcacccac gaactgggga cccctcaacc tcaaggccgg ctcgaccacg cgtcctaccg
     1801 tcaacgacta ccttgacgcg gtcgactaca tagtcgatct cgttggcatc gaccacgtcg
     1861 gcatcggcac cgatatgtcg cacggcacgt accccgacgg cgacaagatc cgcggcctcc
     1921 cgtcgaagcg ctcggttggc ggcgcctacg ccgatcacat cgagcgggcg cctcggtcgc
     1981 gccttcgtta cgtggaaggg ttcgacgact acggacaact tcccacggta gtcgaactca
     2041 tgaagaagcg cggttacagc gaagaggatg tgggcaaggt gctcggaggc aactacctgc
     2101 gggtcttccg tgcggtatgg ggcgcgtgaa cgagaacgcc cggcgcggtc gagctacgac
     2161 gcatacgctc ctcctgtctc agcgcatcgg ggccgagacc gaggaggtct tggcggagcg
     2221 gttcccgggg ctgcggatcg tgcggctccc ggcgagcggg gacgtccctg aggacgcgcg
     2281 cgacgccacc gtgctctatc gggcaggaat gccccacgaa gcactgcgga ccgcgctgat
     2341 ccaactaccc catctcgact ggatccacac ggcctccgcc gggttcaact gggtgttgat
     2401 cccggaagtg atcgccaggc ccatccggtt gacgcgcacg gcccgcgtgc tggacgtccc
     2461 tatcgccgaa tacgtggtcg gcactaccct ggcgctgctg aaaggcctcc cgagcctgct
     2521 cgaggcccaa cgtgcgcggc gctgggagcg cgaggtcgac gcccggggac tgctgggcgc
     2581 cacggtaggc gttatcggcg ccggcgccat cggctcggcc gtggccacgc ggttccgccc
     2641 cttcggcacc aaggtcatcg ggatgaagcg cgatccggcg ccgctggccg agttcgacga
     2701 gatcctcgcg cccggcgagt tggaccgtct tcttaccgat tcggacgtgg tggtcgtagc
     2761 ttgccccttg acgccggaaa cgagacacct gctcggggcg cgggagttcg cgctgatgcg
     2821 gtcacacgcc gtcctggtca acatcgccag gggcgaggtg ctcgtggagg ccgaactcgt
     2881 ggaggcgctg cgcgagcgac gcttcgcggc cgccgcgctg gacgtcttca ccgaggagcc
     2941 gctggcggcc gccagtccgt tgtgggacct cgacaacgtg atcctcacac cgcacatctc
     3001 ctacatcgat ccgacgaact cacttcgcgg ggtggtcgag ttcgccgaca acctggagcg
     3061 ctacctcgcc ggcgcaccgc tactcaacga gatcaagtcg cgtgacctcg ggtactgatc
     3121 ccgtgcccat cgtgatgacg ccaaggagac gaccatgccg acgttcgaag gcctagacag
     3181 cctgccacca gcccccgggc gctaccacgt tcatatgccc gtcgcccgcg acctctccgg
     3241 gcgcaacgtc ggcctcgacg ttatcgtcca ccaaggcgcc gagcccggcc cgacgctgac
     3301 ggtcctctcc ggcctgcacg gcgacgagtg gctgcacctc gagttcttcg ggcgcctgga
     3361 ctcggagatc gaccatgcgg ccctcagggg gcgcgtactg ctcgtacccg tggccaacgc
     3421 ggccgcgttc gccaatcttg cgcgcaacgt cctgcacgag gccgacgcgc cggacatgaa
     3481 cagggtgttc ccggtgacgc cgcgtccgca gaacggcctt cccgaacagc ttgccgccaa
     3541 gctggcgcac gaggttttgg cgcgcagcga cgctctcctc gacttccacc ttggcatctg
     3601 gggttcgacg ctcgcttcca gcatcgtcgg gacc
//
LOCUS       DAOQYE010000080         3631 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_26563_length_3631_cov_2.609899, whole genome shotgun sequence.
ACCESSION   DAOQYE010000080 DAOQYE010000000
VERSION     DAOQYE010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 3631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3631
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26563_length_3631_cov_2.609899"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1014)
                     /locus_tag="PKN52_02225"
     CDS             complement(<1..1014)
                     /locus_tag="PKN52_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HNQ98787.1"
                     /translation="MLSHLLQFWPLLFAFLACAIGTNVLVSIIPRVTGAAFDQVIAGA
                     ADAPALLRVGLLILGLVAARGVLDLTMSFSAETLGQRLERDTRHELYVALLGKSQTFH
                     NRNRVGDIMARAANDVRQLNPMMNPGVALITESVASLVTPMVFIAFIDVRLLLAPALF
                     VVAFYFTLRSYMRQLEPVSTAQREQFGVLNAALNETITGIMVVKSTVREDAERLAFAR
                     NAKAFRDYFVKAGLVQARYLPLLFVGLAVAGALAHGLYLLAHGAISTGDLVAYMGLMS
                     VMRFPAFISIFTFYLVQMAMAGAKRILDLITAEVEMDENATGHAARIEGALTFEGVTF
                     AYEG"
     gene            complement(1289..2428)
                     /locus_tag="PKN52_02230"
     CDS             complement(1289..2428)
                     /locus_tag="PKN52_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfB/YjiJ family MFS transporter"
                     /protein_id="HNQ98788.1"
                     /translation="MTSSTSEQRRELAATLGAAALLGLGPAAAIGVGRFAYALVLPDM
                     LSDLGLSLTQAGLLGSANTGGYLLGALLSHRILRAVDHRRGFYLAAVLQCLTLLALAF
                     APPFMVMAALRLAQGVLGAVVFVGGAALVLAAGGKALSLGLYFGSIGVGMAVSTAVLP
                     LASDWRSGWLWLGVLALALAAASLPAWRRLREPASPVTAGEGSLRPVLAALVSYGLYG
                     AGYIAYMTFVTADLSVATGPFWFVLGLGAVLNGPVWGPVTGRLGGTLAQAAVLLVLLV
                     SSLPPLIGAAPHISALLFGVSFLGVITAITVLIRARLPAGAWPRAMGLSTAAFAAGQA
                     LGPVFTGAVGEAAGGAVGALWTGSVLLALALLVAVFGAARPVGAP"
     gene            2497..>3631
                     /locus_tag="PKN52_02235"
     CDS             2497..>3631
                     /locus_tag="PKN52_02235"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HNQ98789.1"
                     /translation="MRFARQLLSLLIVTVSAIGAAQSMPRETRLAILQAVVQIIPYDT
                     ATDDLVDWSGSGTIISPSGYILTNFHVAGELSTRRAMEWHTIWVTDPDFTDQPPEFYF
                     WAKYVAGDPTHDLALLKIVEWYDEEPVEDDFVFPHVQVGDSNSLIPGDPITIVGYPGI
                     SGSTITFTAGLMSGWVGENFESGGKQWIKTDGKIAHGNSGGGAFDENGYLIGVPTAGR
                     TVKYDDLDVEEQAYVRPISLGWALIGPNVPDVARGGARAPVAQSGNASTSQASGQTSS
                     QQADPSSSGVGPCDFCYVQDLTLGDKVSYTIGGVPDYINFHSYSVNVPEGTREFTVLL
                     DSEGDLDLAVKYGTPIESWGDEGDWDYRDITEAPGGAFTITQPT"
BASE COUNT          643 a         1263 c         1249 g          476 t
ORIGIN      
        1 gccctcgtag gcgaacgtga caccctcgaa cgtcagcgcg ccctcgatcc gagcggcgtg
       61 ccccgtcgcg ttctcgtcca tctcgacctc ggccgtgatg aggtcgagga tgcgcttggc
      121 gccggccatg gccatctgaa cgaggtagaa ggtgaagatc gagatgaacg ccgggaagcg
      181 catgaccgac atgaggccca tgtaggcgac gaggtccccg gttgagatgg cgccgtgggc
      241 cagcaggtag aggccgtggg cgagggcgcc cgccacggcg agcccgacga acagcagggg
      301 caggtaccgc gcctgcacga gacccgcctt cacgaagtag tcgcggaaag ccttggcgtt
      361 acgcgcgaac gccaggcgct cggcgtcctc gcgcacggtc gacttgacga ccatgatgcc
      421 cgtgatggtc tcgttgagcg ccgcgttgag gacgccgaac tgctcgcgct gcgccgtcga
      481 gacaggctcg agctggcgca tgtacgagcg cagggtgaag tagaaggcca ctacgaacag
      541 ggcaggcgcg aggaggaggc ggacgtcgat gaacgcgatg aacaccatgg gtgtcacgag
      601 gctggcgacg gactcggtga tgagcgcgac cccggggttc atcatcgggt tgagctggcg
      661 gacgtcgttg gcggcccgcg ccatgatgtc tccgacgcgg ttgcggttgt ggaacgtctg
      721 gctcttgccg agcagcgcga cgtagagttc gtggcgcgtg tcgcgttcca gcctctgacc
      781 gagggtctcg gctgagaagc tcatcgtcag gtcgagcacc ccgcgggcgg cgaccaggcc
      841 gagtatgagc agcccgaccc tcagcagcgc gggggcgtcg gcggcgcctg cgatcacctg
      901 gtcgaaggcg gcgcccgtca cgcgcgggat gatggagacg agcacgttcg tgccgatggc
      961 gcaggccagg aaggcgaaga ggagcggcca gaactgcagc agatgggaga gcacccacct
     1021 gagcggactg cggcggtcgt agcgccggac gccggctacg gcgaactcgc cctgcgcctg
     1081 agggtcttga ggccgggggt ccttctgctg cggacctcgg ggctgggcac cctggcctct
     1141 gagtgtcgtg gtcgggatag ccatcggctg cggccctccg aaggccgagg gacagtttag
     1201 accggcgaag ttgctttcgg tccagcgagc gtcagggcgc cccgaagctc cgctcctcga
     1261 actgccacga cccgtggctg caagagcctc acggcgcccc gaccgggcgc gccgcgccga
     1321 acaccgcgac cagcagggcg agcgccagta ggacgcttcc cgtccagagc gccccgacgg
     1381 cgcctccggc ggcctcgccg acggcgccgg tgaagacggg gccgagcgcc tggccagcgg
     1441 cgaacgccgc ggtggagagg cccatcgccc gcggccacgc tccggccggc aggcgcgccc
     1501 tgatcaggac cgttatcgca gtgatgacgc cgaggaacga gacgccgaag agtagcgccg
     1561 agatgtgcgg cgccgcgccg atcaggggcg gcaggctgga gacgaggagc acgaggagca
     1621 cggctgcctg cgccagcgtt ccgccgagcc tgcccgtgac gggtccccag accggaccgt
     1681 tcaagacggc cccgagtccg agtacgaacc agaaggggcc ggtggccacg ctcaggtcgg
     1741 cggtcacgaa cgtcatgtag gcgatgtacc cggccccgta gaggccgtag ctgaccagcg
     1801 ccgcgagtac ggggcgcaag ctgccctccc ccgccgtcac cggcgaggcc ggctcccgga
     1861 gccgccgcca ggccggcaag ctcgcggccg cgagcgccaa cgccagcaca ccgagccaca
     1921 gccagccgga ccgccagtcg gaggcgaggg gcagcaccgc cgtggagacg gccatgccga
     1981 cgccgatgct gccgaagtag agcccgaggc tcaaggcctt gccgccggcc gccaggacga
     2041 gcgccgcccc tcccacgaac acgacggcgc ccagcacccc ctgagcgagc cgtagggcgg
     2101 ccatgaccat gaacggcggg gcgaacgcga gcgcgagcag cgtcaggcac tggaggaccg
     2161 ccgccaggta gaagcctctc cggtgatcga cggcccgcag gatccggtgg ctgaggagcg
     2221 ctcctagcag gtatccgccc gtgttggcgc tgcccaacag ccccgcctgc gtgaggctca
     2281 accccaggtc ggacagcatg tctggcagca ctagcgcgta ggcgaacctg ccgaccccga
     2341 tggcggccgc cgggccgaga ccgagcagcg cggccgctcc cagcgtggcc gccagctccc
     2401 tgcgctgctc agatgtagac gatgtcacgt gctagacggt atcaggtccg gctacaatgc
     2461 ggcacgactc ccccttcgcc cgagaggatg ccgccaatgc gcttcgcgcg tcaactgcta
     2521 agcctgctga tcgtcacggt gagcgccatc ggcgccgccc aatcgatgcc ccgagagacc
     2581 cgactcgcca tcctccaggc cgtcgtacag atcatcccgt acgacaccgc cacggacgat
     2641 ctcgtggact ggtctggctc gggcaccatc atcagcccgt ccggttacat cctcacgaac
     2701 ttccacgtgg ccggcgaact cagtacccgc cgcgccatgg aatggcacac catctgggtc
     2761 accgaccccg acttcacgga ccagccgccc gagttctact tctgggccaa gtacgtggca
     2821 ggcgacccga cccacgacct cgccctcctc aagatcgtcg agtggtacga cgaggagccc
     2881 gtcgaggacg atttcgtctt cccgcacgtg caggttggcg actcgaacag tctcatccct
     2941 ggcgatccca tcaccatcgt cggctacccg ggcatctccg gctccaccat caccttcacg
     3001 gcaggtctca tgtccggctg ggtcggcgag aacttcgaga gcggcggcaa gcagtggatc
     3061 aagaccgacg ggaagatcgc ccacggcaac tcgggcggcg gggcgttcga cgagaacggt
     3121 tacctgatcg gcgtcccgac cgccggacgc accgtcaagt acgacgacct cgacgtcgag
     3181 gaacaggcct acgtcaggcc gatcagcctc ggctgggccc tcatcgggcc gaacgtgccg
     3241 gacgtcgcgc gcggcggcgc cagggctccc gtcgcgcagt ccggcaacgc ctccacgagc
     3301 caggcgagcg gccagaccag cagccagcag gccgacccga gcagcagcgg cgtcggtccg
     3361 tgcgatttct gctacgtcca ggacctgacc ctgggcgaca aggtgtccta caccatcggc
     3421 ggcgtgcccg actacatcaa cttccactca tacagtgtga acgtgccgga agggacccgc
     3481 gagttcaccg tcctgctcga ctccgagggc gacctcgacc tcgcggtcaa gtacggcacg
     3541 cccatcgagt catggggcga cgaaggcgac tgggactacc gcgacatcac ggaggcgccg
     3601 ggcggagcct tcaccatcac gcagccgacg c
//
LOCUS       DAOQYE010000081         3629 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_26587_length_3629_cov_3.226357, whole genome shotgun sequence.
ACCESSION   DAOQYE010000081 DAOQYE010000000
VERSION     DAOQYE010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 3629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3629
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26587_length_3629_cov_3.226357"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            226..618
                     /locus_tag="PKN52_02240"
     CDS             226..618
                     /locus_tag="PKN52_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98790.1"
                     /translation="MTNLEDVLAGLWAQLPGCRVAAVVGMDGLLVERHPSVDVGVALG
                     SAPGGDELEYLAADLTTAFTLVGGELSKQLGSRIDEFVALGESGGYLARRVDANLFLF
                     VVVGATADLGVVRRAVAGVCEQLAAEFA"
     gene            615..1142
                     /locus_tag="PKN52_02245"
     CDS             615..1142
                     /locus_tag="PKN52_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98791.1"
                     /translation="MSGGLPADAESVGGADPAGQAALAADQSGVRGRSAVAESFAALL
                     EGLLAVRGATVAAVADGGGEILASRAADDAALQRTTSVVTSALAAATALTGLLAAGSG
                     AAAGTGGPTASAASSLPRQVMVNLDAGLILLVPLGTSEHVLVLGLASESDVGRARFAL
                     KGIVAKAAKLLSEPA"
     gene            1190..1498
                     /locus_tag="PKN52_02250"
     CDS             1190..1498
                     /locus_tag="PKN52_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HNQ98792.1"
                     /translation="MNPIKFEIDKETARGRPTNGAVIAHTPDEFLLDFVLVVPGQQPV
                     VTARLVSSPRHAKALLRSLEDNIKRYEARFGAIPEPVVQKEGSVRPTVPGSDGGEDPN
                     "
     gene            1553..2242
                     /locus_tag="PKN52_02255"
     CDS             1553..2242
                     /locus_tag="PKN52_02255"
                     /inference="COORDINATES: protein motif:HMM:NF013488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HNQ98793.1"
                     /translation="MQERVTVYFDYICPYSWRAAELFDLIAQPLALDVRWEHFSLFQN
                     EHGQGVAIAGATAGAVAGATAGSQAGAKADAWQLWNERIDEKDGYGCKGLVPFLASLA
                     ARKQGPEAHAAFRLALQRANHAHFRPFSINMVTSVAESVGLDVQRFQHELHNPEARTV
                     LAQEHMRAVARDVFGTPTVVFPSGQTAYLRLQEQPADVEEATQLFQDVRRLLERYPYL
                     QTVRRPRAKGN"
     gene            2289..3056
                     /locus_tag="PKN52_02260"
     CDS             2289..3056
                     /locus_tag="PKN52_02260"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HNQ98794.1"
                     /translation="MRLGHNVGHGSDVGRVRPFNEDFHRVWQFPMEGGDLTLLAVADG
                     MGGAAAGEVASKLAMQVLDESFSRYVEEVRSRRPVVGVLSLIDKAMRLANRRVYGEAI
                     KQPGRRGMGSTLTCVAIRKRKAYIGHVGDSRAFLVRGNKIYQLTKDHSWVEEQVEKGL
                     LNEEEAENHEWRNLITRVLGTRQQVAPDVVELDVQSGDVFLLSTDGLHGLVSPEEMLA
                     EIQENSDRQLNVEVLLAQANERGGPDNITVVIFEVDT"
     gene            3073..>3629
                     /locus_tag="PKN52_02265"
     CDS             3073..>3629
                     /locus_tag="PKN52_02265"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNQ98795.1"
                     /translation="MILLWVVGAALVALLFRAPVWVLGVLLTVAVLLTGGVLWLGGAE
                     RALTPLILTVLALGLLVPATVQVARSSRVAPARRGPKRAADAKATAGGGPQQQFDAAD
                     VPGYTLLEKVGSGGMASVYRARRASDGQIVALKIPMEQYVADAKFIRRFHREAEVAQR
                     LNHTNIVRTVEHGAVGVQHYMVMEFV"
BASE COUNT          550 a         1250 c         1286 g          543 t
ORIGIN      
        1 tagccgtaga ggtggcggta agagttgtag cgcttcgggt cgtccaggaa gcgctctgcc
       61 accagggtgg cgcggtctgg gtcgccgggg aggagcacga cgggagccac gctgccttcc
      121 gccgctcgca catggagttg cgtcatcggc ctcacgatac cagccgccca catatgaaca
      181 gccatacgga gcgcgcgctg ggtcgggtgc tagagtgccg ccggtatgac gaacctcgag
      241 gacgtcctgg ccggtctctg ggcgcagctg cccggctgtc gtgtcgccgc tgtcgtcggc
      301 atggacgggc tcctggtcga gcgccaccct tccgtggacg taggcgtcgc actcggctca
      361 gcgccgggcg gcgacgagtt ggagtacttg gccgccgacc tgaccaccgc cttcacgctc
      421 gtcggcgggg aactgagcaa gcagttgggc tcgcgtatcg acgagttcgt ggcgctcggc
      481 gagtcgggcg gttatctggc gaggcgggtc gacgccaacc tcttcctctt cgtcgtggtc
      541 ggcgccaccg ctgacctcgg cgtggtcaga cgcgccgtcg ccggggtctg cgagcagctc
      601 gccgcggagt tcgcgtgagc ggcgggcttc cggccgatgc cgaaagcgta ggcggtgccg
      661 acccggccgg ccaggctgct ctagccgccg accagtccgg cgtgcgcgga cgctccgccg
      721 tggcggagag cttcgccgcc ctcctggagg ggctgctggc cgtgcgggga gccactgtgg
      781 ccgccgtggc cgacggcgga ggggagatcc tggcatcgcg cgcggcggac gacgccgcgc
      841 tgcagcgcac gacgagcgtc gtgacgagcg cgctggccgc cgcaaccgcc ctgacggggc
      901 tgctggccgc agggtccggc gcagccgccg ggaccggcgg gccgaccgcg tcggctgcga
      961 gctccctccc gcggcaggtc atggtgaacc tcgacgccgg cctgatcctg ctcgtgccct
     1021 taggcacgag cgagcacgtg ctcgtgctcg gcctcgcctc cgagtccgac gtcggcaggg
     1081 cgcggttcgc cctgaagggc atcgtggcca aggccgccaa gctcctgagc gaacccgcct
     1141 gacgccaggg cgaacccgcc tgaggcgccc ggttgggtac cctggtcggg tgaaccccat
     1201 caagttcgag atcgacaagg agacggcgcg cgggaggccg accaacgggg cggtcatagc
     1261 gcataccccc gacgagttcc tcctcgactt cgtcctggtc gtgcccggcc agcagccggt
     1321 cgtcacggcc aggctggtgt ccagcccgcg tcatgccaag gcgctgctga ggagcctgga
     1381 ggacaacatc aagcgctacg aggcgcgttt cggcgcgatc cccgagccgg tggtgcagaa
     1441 ggaaggctcg gtccgcccta cggtgcccgg ctcggacggc ggcgaagacc ctaactgacc
     1501 gcctgtcacg tcggctacga cctgacgggc gcgccggtgg tctcatgacc gcatgcaaga
     1561 gcgcgtcacc gtctacttcg actacatctg cccgtactcg tggcgcgcgg ccgaactctt
     1621 cgacctgatc gctcaaccgc tcgctctcga cgtgcgctgg gagcacttct cgctgttcca
     1681 gaacgagcac ggccagggcg tcgccatcgc gggcgccact gcgggcgccg ttgcgggggc
     1741 cactgcgggc tcccaggcgg gcgccaaggc ggacgcgtgg cagctctgga acgagcgcat
     1801 cgacgagaag gacggttacg gctgcaaggg cctggtgccg ttcctcgcgt cgctggccgc
     1861 gcgcaagcaa ggaccggagg cgcatgccgc gttccggctc gcgctccagc gcgccaatca
     1921 cgcgcacttc cggccgttct ccatcaacat ggtcacgtcc gtggccgagt cggtcgggct
     1981 cgacgtgcaa cgcttccaac acgagctgca caaccccgag gcccgcacgg tgttggccca
     2041 ggagcacatg cgggccgtgg cgcgcgacgt cttcgggact ccgacggtag tgttcccctc
     2101 aggacagacg gcttacttga ggctgcagga gcagccggcc gacgtcgagg aggccaccca
     2161 gctcttccag gacgtcaggc ggctcctgga gcggtacccg tacctgcaga cggtgcggcg
     2221 gccacgcgcc aagggcaact gagcgaaaaa cccgccttag gcactccgaa gcaatgtaga
     2281 ctcgtggcgt gcgcctcggt cacaacgtgg gtcacgggtc cgatgtcgga cgcgtccgac
     2341 ctttcaacga ggacttccac cgcgtctggc agttccccat ggaggggggc gacctgacgc
     2401 tcctggcggt cgccgacggc atgggcggcg ccgctgccgg cgaggtcgcc agcaagctcg
     2461 ccatgcaggt gctcgacgag tcgttctccc gttacgtcga ggaagtgcgc tcccgccggc
     2521 cggtggtcgg cgtcctgagc ctcatcgaca aggccatgcg cctggccaac cggcgcgtgt
     2581 acggcgaggc catcaagcaa cccggcaggc gcggcatggg gagcaccctc acctgcgtgg
     2641 ccatccgcaa gcgcaaggcc tacatcgggc acgtcggcga ctcgcgcgcc ttcctggtgc
     2701 gtggcaacaa gatctaccag ctcaccaagg accactcctg ggtggaggag caggtggaga
     2761 agggcctcct gaacgaggaa gaggccgaga accacgagtg gcgcaacctg ataacgcgcg
     2821 tgctcggcac gaggcagcag gtcgcccctg acgtcgtcga gctcgacgtg cagtcgggcg
     2881 acgtcttcct cctctcgacg gacgggctcc acggcctcgt atcgccagag gagatgctcg
     2941 ccgagatcca ggagaactcg gacaggcagc tcaacgtgga agtgctgctc gcgcaggcca
     3001 acgagcgcgg cgggccggat aacatcacgg tggtgatctt cgaggtggac acgtgacctg
     3061 cgaggcgagc gcatgatcct gctatgggtc gtcggcgccg cgctcgtggc gttgctcttc
     3121 agggcgcccg tctgggtgct cggcgtgctc ttgaccgtcg cggtgctcct caccggcggc
     3181 gtgctgtggt tgggcggcgc ggaacgcgcc ctcaccccgc tcatcctgac ggtcctcgcc
     3241 ctcggcctgc tcgtacccgc caccgtccag gtggcgcgga gcagccgcgt ggcgcccgcg
     3301 cggcgcggtc cgaagcgcgc ggctgacgcc aaggcgacgg ccgggggagg cccgcagcag
     3361 cagttcgatg ccgccgacgt gccgggctac acgctcctcg agaaggtcgg ctccggcggc
     3421 atggccagcg tctatcgcgc caggcgcgcc agcgacggcc agatcgtcgc cctgaagatc
     3481 ccgatggaac agtacgtggc cgacgccaag ttcatccgtc gcttccaccg tgaggcggag
     3541 gtggcccagc gcctcaacca cacgaacatc gtccgcaccg tcgagcacgg cgcggtcggc
     3601 gtgcagcact acatggtcat ggagttcgt
//
LOCUS       DAOQYE010000082         3623 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_26650_length_3623_cov_2.207399, whole genome shotgun sequence.
ACCESSION   DAOQYE010000082 DAOQYE010000000
VERSION     DAOQYE010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 3623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3623
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26650_length_3623_cov_2.207399"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="PKN52_02270"
     CDS             complement(<1..623)
                     /locus_tag="PKN52_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase domain-containing
                     protein"
                     /protein_id="HNQ98796.1"
                     /translation="MSLLSKPPALLALEDGTVYYGYAFGAHGKSVGEIVFNTSMTGYQ
                     EILTDPSYHGQIVTMTYPHIGNYGVSVYDMESNRPYARGFIVREFSRVASNHRANQDL
                     QAFMEQHGIVGIEGIDTRALTRRLRSGGVVKGVIYHGHTDDALEAELVQEARDHEDID
                     GRDMTPEVTTPLPYARPTFQDHPRVVLIDFGIKHSIVYKLEHSGAEV"
     gene            complement(636..1625)
                     /locus_tag="PKN52_02275"
     CDS             complement(636..1625)
                     /locus_tag="PKN52_02275"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HNQ98797.1"
                     /translation="MTDGDAPGATPLAVVVLAAGEGKRMRSKLHKVLHEAAGAPLLQH
                     ILDAVAPLNPSRTVVVVGFSGETVRERFEGAGVTFVTQDFTTGYGTGHALRESRKGLE
                     GFSGNVLVLNGDGPLLRSRTLADLEGLLGGERGMALLTCRTADPTGLGRIVRGPDGEL
                     QAIVEEKDATPEQRAIREINPGVYLFDDDVFRRAAELRNDNAAGEYYITDLPRGYLDD
                     GLKVRTLLADERELLGVNDRRQLAVAERILRDRYVDAWLTAGVTMLDPATTYLDANVE
                     LAPDVVLEQGVTLRGRTRVGEGARVGAYSVLTDVLVPAGASVPPHTVRTGQDW"
     gene            complement(1622..2635)
                     /gene="lgt"
                     /locus_tag="PKN52_02280"
     CDS             complement(1622..2635)
                     /gene="lgt"
                     /locus_tag="PKN52_02280"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNQ98798.1"
                     /translation="MDPILIQLGPLAIRWYGLLIALGVLVGSGIALRQAERRGLDQEK
                     LLDMAVWLVIAGIVGARLVYVLTSPSAFFGPGGNPLDAVKVWEGGISIHGGVLGIVLA
                     TWWYCRAHKLDMWAYLDVMSPIAGLGIIGGRIGNFMNGTDTGGRLTNWPIGFTWPEPG
                     TQTLGAFGRAVFGRDLWSAFPGVCSDGSYIPIWQCTGEIVRGPVHLTQLYGVIIGVLV
                     LLITLWALRQRRGSGFVFWQMVLWYSVLRSVLEETFRDNPLSLNLVLADGLDKAGVGL
                     FTMTQLASVVIIAVAVYMLIRIGRAPARAPVTGPGAGPSRSGSRQVGRATAPTEKRKG
                     KSR"
     gene            complement(2709..3200)
                     /locus_tag="PKN52_02285"
     CDS             complement(2709..3200)
                     /locus_tag="PKN52_02285"
                     /inference="COORDINATES: protein motif:HMM:NF016213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HNQ98799.1"
                     /translation="MLVTILAFAVFLVSYVVAALGVVLPVLPGVPIAAVGAVAAAAIM
                     GFERFGVTVLIHVGLLVVLSQVVDLAGTWLGSRHYGAGRAGTWGGILGSFVGLFAFPP
                     WGFLFGALVGAVVAELLIGRELSAAIRSGIGAFVGTLGGTVAKLVIMVVIGVIVFPRF
                     FSG"
     gene            complement(3211..>3623)
                     /locus_tag="PKN52_02290"
     CDS             complement(3211..>3623)
                     /locus_tag="PKN52_02290"
                     /inference="COORDINATES: protein motif:HMM:NF013183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HNQ98800.1"
                     /translation="KGGPFVEVDYQQVPDEQALIRAKLRVWCDQGNIDLVLTTGGVGL
                     GQRDRTPEATAEVIERHVPGLPEALRVALLGEQPLASLYRALAGVRRSTLIMNLPGTP
                     ESAAKGLAAVVTALAFAVEELRGEAAKGDVQTSA"
BASE COUNT          589 a         1238 c         1275 g          521 t
ORIGIN      
        1 atcacctcgg cgccggagtg ctccagcttg tagacgatgg agtgcttgat gccgaagtcg
       61 atgagcacca cgcgcgggtg gtcctggaag gtcgggcggg cgtaaggcag cggcgtggtc
      121 acctcgggcg tcatgtcgcg cccgtcgatg tcctcgtggt ccctggcctc ctgcacgagc
      181 tcggcctcga gggcgtcgtc ggtgtggccg tgatagatga cgcctttgac gacgccgccg
      241 gagcgcaacc ggcgcgtgag ggcgcgggtg tctatcccct cgatgccgac gatgccgtgc
      301 tgctccatga acgcctggag gtcctggttc gcgcggtggt tgctcgcgac gcggctgaac
      361 tccctgacga tgaaaccccg cgcgtacggt cggttcgact ccatgtcgta gacgctcacc
      421 ccgtagttgc cgatgtgggg gtaggtcatc gtcacgatct gaccgtggta gctggggtcc
      481 gtgaggatct cctggtaacc ggtcatagac gtgttgaaga cgatctcgcc gacggacttg
      541 ccgtgggcac cgaaggcgta gccgtagtag acggtgccgt cctcgagggc gagcagcgcg
      601 gggggtttgc tcaggagtga catcgaacgt ctaccctacc agtcctggcc ggtcctgacc
      661 gtgtgtggcg ggacggaggc gccagccggc accagcacgt cggtcagcac cgagtacgcc
      721 ccgacccgtg cgccctcccc gacacgcgta cgaccgcgca gggtgacgcc ctgctccagc
      781 acgacgtcgg gggccagctc cacgtttgcg tcgaggtagg tggtggccgg gtcgagcatc
      841 gtcacgccgg cggtcaacca ggcgtccacg tagcggtccc gtaggatccg ctccgccacg
      901 gcgagttgcc gcctgtcgtt gacaccgagg agctcgcgct cgtcggcgag cagcgtgcgc
      961 accttcagcc cgtcgtcgag gtagccgcgc gggaggtcgg tgatgtagta ctcgccggcc
     1021 gcgttgtcgt tgcgcagctc ggccgcgcgc ctgaacacgt cgtcgtcgaa gaggtaaacg
     1081 ccggggttga tctcacggat ggcgcgctgc tcgggcgtgg cgtccttctc ctcgacgatg
     1141 gcctggagct cgccgtccgg tccgcgcacg atgcgcccga gaccggtagg gtcggccgtg
     1201 cggcaggtca ggagcgccat gccgcgctcg ccgcccagga gcccttcgag gtccgccagc
     1261 gtccggctcc tcagcaacgg gccgtcgccg ttgagcacga gcacgttgcc gctgaaaccc
     1321 tccaacccct tgcggctctc gcgcagggcg tggccagtgc cgtaaccggt cgtgaagtcc
     1381 tgggtgacga acgtgacgcc agcgccctcg aagcgctcgc ggaccgtctc acccgagaaa
     1441 cccaccacta cgacagtgcg gctcgggttc aacggggcga ccgcgtccag gatgtgttgc
     1501 aggagcgggg cgccggccgc ctcgtgcagg accttgtgaa gcttggagcg catgcgcttc
     1561 ccttcgccgg cggccaggac gaccacggcg aggggggtag cgcccggcgc atctccatcc
     1621 gtcatctgga cttccctttc cgcttctccg tcggcgccgt ggcgcggccg acctggcgcg
     1681 agccggagcg cgacggcccg gcgccgggcc cggtaaccgg cgcgcgggcc ggtgcccggc
     1741 cgatcctgat cagcatgtac acggccacgg cgatgatcac gacgctcgcc aactgggtca
     1801 tggtgaagag ccccacaccg gccttgtcaa ggccgtccgc gagcaccagg ttcaggctga
     1861 gggggttgtc gcggaacgtc tcttcgagga cggagcgcag cacggagtac cagaggacca
     1921 tctgccagaa cacgaagccg gacccgcgcc gctgccgcag cgcccagagc gtgatcagga
     1981 ggacgaggac gccgatgatc acgccgtaga gctgagtgag gtgcacgggg ccgcgcacga
     2041 tctcccccgt gcactgccag atcggtatgt acgagccgtc cgagcagacg ccggggaagg
     2101 cgctccagag gtcgcgcccg aacacggccc gaccgaacgc cccgagcgtc tgcgtgcccg
     2161 gctcgggcca cgtgaagccg atgggccagt tcgtgaggcg cccgcccgtg tcggtgccgt
     2221 tcatgaagtt gccgatgcgc ccgccgatga tgccgaggcc ggcgatgggc gacatgacgt
     2281 cgaggtaggc ccacatgtcg agcttgtgcg cgcggcagta ccaccacgtg gcgaggacga
     2341 tgccaaggac gccaccgtgg atgctgatgc cgccctccca gaccttgacg gcgtcgagtg
     2401 ggttgccgcc gggtccgaag aaggccgacg ggctcgtcag gacgtagacg aggcgcgcgc
     2461 cgacgatgcc ggcgatgacg agccagacgg ccatgtcgag gagtttctct tgatcgaggc
     2521 cacggcgctc ggcctggcgc aacgcgatgc cggagccaac gagcaccccc agtgcgatga
     2581 gcagcccgta ccaccggatc gctagcggac caagctggat gaggatcgga tccatgcgac
     2641 tcctaacgcc acgctcgttg gcggctgccg gtacgaccgg acccccgccg cggcgcttct
     2701 aaggcgtgtc agcccgagaa gaacctcggg aacacgatca cgccgatgac caccattatg
     2761 acgagcttgg cgaccgtgcc gcccaacgtg ccgacgaagg cgcctatccc ggacctgatg
     2821 gcggcgctca actcccgccc gataagcagc tcggcgacga cggcgcccac cagggcgccg
     2881 aacaggaagc cccacggcgg gaacgcgaag agcccgacga aggagcctag tatgccgccc
     2941 cacgtgcccg ccctgccggc gccgtaatgc ctggaaccga gccaggttcc ggcgagatcg
     3001 acgacctgcg agaggacgac gagcagcccg acgtggatca acacggtgac accgaagcgc
     3061 tcgaagccca tgatggcggc ggcggcgacc gcgccgacgg ccgcgatggg cacgccgggc
     3121 agcacgggca gcaccacccc gagcgccgcc acaacgtacg agacgaggaa gaccgcgaac
     3181 gcgaggatgg tcacgagcat ggcgagccgt tcaggccgac gtctggacgt cgcccttggc
     3241 cgcctcgccg cggagctcct cgaccgcgaa cgcgagggca gtgaccacgg cggcgaggcc
     3301 cttggcggcc gactcgggcg tgccaggcag gttcatgatc agcgtgctgc gcctgacgcc
     3361 ggcgagcgcg cggtagagcg acgcgagggg ctgctccccc aggagcgcca cccttagggc
     3421 ctcgggcaga ccgggcacat ggcgctcgat gacctccgcc gtcgcctcgg gcgtgcggtc
     3481 gcgctggccc aggccgacgc cgcccgtggt gagcacgagg tcgatgttgc cctggtcgca
     3541 ccacacgcgc agcttggccc ggatgagggc ctgctcgtcc ggcacctgct ggtagtcgac
     3601 ctccacgaac ggtccaccct tga
//
LOCUS       DAOQYE010000083         3595 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_26938_length_3595_cov_2.940678, whole genome shotgun sequence.
ACCESSION   DAOQYE010000083 DAOQYE010000000
VERSION     DAOQYE010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 3595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3595
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26938_length_3595_cov_2.940678"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1698
                     /locus_tag="PKN52_02295"
     CDS             <1..1698
                     /locus_tag="PKN52_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HNQ98801.1"
                     /translation="AAPVHTLEPGKITRGAWALARTLMDRGVCVIDGYIGVDWDGLRA
                     ELSAMLVEAGAQVRWLDVREALKPAAEIEALVEPYLGGSDPVFGRRHPGALADLYDSA
                     ALARLRPAPDGVSILYGCGAALAGWQAFTAYVELPKDTIQHRAKAGAATNLGRVAPEN
                     AKAMYKRFYFVDWPLLNRHKQELLPRIDLVIDGQRTRDPSFMAGDDLREELGRMSRGC
                     VRARPWFTPGPWGGQWLKEIVPQLPQDVPNYAWSFELIAPENGLTMESGGERLEVAFD
                     CLMFHAAEAVLGAHAERFGTDFPIRFDYLDTMGGGNLSVQVHPRPDYIREEFGEPFTQ
                     DETYYLVDCEPGAVVYLGFQDGVDPAEFRARLEESAAAGTPVAVEKFVRTFPAERHGL
                     YLIPHGTIHCSGAGNVVLEVSATPYIYTFKMYDWLRLDLDGKPRPLNVARAFDNLNFD
                     RQGQEAESLVSRPALIDEGPGWKIVHLPTHKDHFYDLHRVELESAVELVTDASPNVLA
                     VVEGGPVRLRTAEGDVVCRFAETYVVPAAAGRYELVNEGRGEAKIVKAFLKPLAGAS"
     gene            1695..1823
                     /locus_tag="PKN52_02300"
     CDS             1695..1823
                     /locus_tag="PKN52_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98802.1"
                     /translation="MTRAATLPTLDLVARCREADRRERPRYLIDSLADGRSAVTAP"
     gene            complement(1810..2478)
                     /locus_tag="PKN52_02305"
     CDS             complement(1810..2478)
                     /locus_tag="PKN52_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98803.1"
                     /translation="MSQKVRIAAAIALVLALPTVVMAQEDPAAEEALAQAVSALNGSS
                     FHFAYSVWPAQGANTPITGEGDFDRSTQSFSYNVVTDEGDATVDHANDGSWRMVGGVM
                     YHDAGNGWQTGEMDFTHMAVTGMIAPFESYPFAKMFGSPEDGNLELLALAGTDEVDGV
                     QAAHYRFSTKELEVLGTGVYEAWVAGDPASPSFVVLQSTDADGKVNRVVYSALGEDVT
                     ITAP"
     gene            2715..>3595
                     /locus_tag="PKN52_02310"
     CDS             2715..>3595
                     /locus_tag="PKN52_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S10"
                     /protein_id="HNQ98804.1"
                     /translation="MPNGSEQPTPTAAATAAPETGARTPKDNLVVTRHETTILGKKVS
                     YTATTGTIVLSEEAVGEGEKAGKFEGVKPRAEVFFVAYELDGVTDKSKRPVTFSFNGG
                     PGSSSVWMHLGLFGPKRVELDDDGYPSAPPYRLVQNDVSLLDVTDLVFIDPVSTGYSR
                     PVEGVKAKDFHNVDADVESVGAFIRLYTGRYGRWTSPKYLAGESYGTTRAAGLAGHLQ
                     ERHGLYLNGLILVSAILDFSTARFGPGNDLPPLMFLPTYAAAAHYHGQLDAELQALPL
                     SVLLERVEVFVHERYAPA"
BASE COUNT          584 a         1211 c         1221 g          579 t
ORIGIN      
        1 gcggcgccgg tccatacgct cgagcccggc aagatcacgc gtggggcgtg ggcgctggcg
       61 cggactctca tggaccgggg cgtctgcgtg atcgacggtt acataggggt cgactgggat
      121 gggctgcgcg ccgagctgag cgccatgctg gtcgaggcgg gcgcccaggt caggtggctg
      181 gacgtgcgtg aggcgctcaa gccggcggcc gagatcgagg cgctcgtcga gccgtacctc
      241 ggcggctccg acccggtgtt cggcaggcgc cacccgggcg cgctcgcgga cctttatgac
      301 tccgcggcgc tggcgcgtct gcggcccgcc ccagacgggg tatcgatcct ctacggttgc
      361 ggggcggccc tcgccgggtg gcaggcgttc accgcctacg tggaactccc gaaggacacg
      421 atccaacacc gcgccaaggc cggtgcggcg accaacctcg gacgcgtggc gccggagaac
      481 gccaaggcga tgtataagcg cttttacttc gtcgactggc cgctactcaa ccggcacaaa
      541 caggagctgc tgccgcgtat cgatctggtg atcgacggcc agcggactcg agaccctagc
      601 ttcatggccg gcgacgacct aagggaggaa ctagggcgga tgagccgcgg ctgcgtgcgc
      661 gcccgcccct ggttcacgcc cggtccgtgg ggcggtcaat ggttgaagga gatcgtgccg
      721 cagcttccgc aggatgtccc caactacgcc tggtcgttcg agttgatcgc ccccgagaac
      781 gggctgacca tggagagcgg cggggagcgg ctcgaggtgg cgttcgactg cctgatgttc
      841 cacgcggcag aggcggtgct cggcgcgcat gccgagcgtt tcggcaccga cttcccgatc
      901 cgcttcgact acctggacac catgggcggt ggcaacctct cggtgcaagt ccacccacgg
      961 cccgactaca tccgggaaga gttcggcgag ccgttcaccc aggacgagac ctactacctg
     1021 gtcgactgcg aacccggcgc agtcgtttac cttgggttcc aggacggcgt tgacccagcc
     1081 gagttccgcg ctcggctgga ggagagcgcc gccgccggca cgccggtggc tgtcgagaag
     1141 ttcgtgcgga ccttccccgc cgagcggcac ggtctgtact tgataccgca cggcactatc
     1201 cactgctccg gggcgggcaa cgtggtgctc gaggtgagcg cgacgccgta catctacact
     1261 ttcaagatgt acgactggct gcggctcgac ttggacggca aaccgcgccc gctcaacgtg
     1321 gcacgcgcgt tcgacaacct gaacttcgac cggcagggac aggaggccga gtctctggtg
     1381 tcgcggcccg ctctcatcga cgaggggcca ggctggaaga tcgtgcacct tcccacgcac
     1441 aaggaccact tctacgactt gcaccgggtg gaactcgagt cagcagtgga actcgttaca
     1501 gacgcttctc ccaacgtcct ggcggtagtg gagggcggcc ccgtgcggct gcggaccgcc
     1561 gagggcgacg tcgtctgccg tttcgccgag acatatgtag tgccggccgc ggccggccgg
     1621 tacgagctcg tgaacgaagg gcggggcgag gccaagatcg tgaaggcgtt cctcaagccg
     1681 ttggcgggag cgtcgtgacg cgcgcggcca ccttgccgac gttggacctg gtggcgcgct
     1741 gccgcgaggc ggacaggcgc gaacggccgc gctacctcat cgactccctc gcagacggcc
     1801 ggtccgccgt cacggcgccg tgatggtgac gtcctcgccg agcgcgctgt agaccactct
     1861 gttcaccttg ccgtcggcat cggtgctctg caggaccacg aacgagggcg aggcggggtc
     1921 gccagccacc caggcctcgt agacgccggt tccaagcact tcgagctcct tggtcgagaa
     1981 gcggtagtgc gccgcctgca cgccgtcgac ctcatcggta ccggccaacg ccagtagttc
     2041 gaggttgccg tcctccggcg agccgaacat cttcgcgaac gggtagctct cgaacggggc
     2101 gatcatgccg gtgacggcca tgtgcgtgaa gtccatctcc ccggtctgcc agccgttgcc
     2161 ggcgtcgtga tacatgacgc ccccgaccat gcgccacgac ccgtcgttgg cgtggtcgac
     2221 ggtcgcgtcg ccctcgtcgg tgaccacgtt gtaactgaag ctctgggtgg accggtcgaa
     2281 gtcgccctcg cctgtgatgg gcgtgttcgc tccctgcgcg ggccagacgc tgtaggcgaa
     2341 gtggaagctg gagccgttga gggcgctcac agcctgggcg agagcctcct cggcggcggg
     2401 atcttcttgc gccatcacca ccgtcggcag cgccaagacc agcgctatgg cggctgctat
     2461 gcgaaccttt tgagacatag gaatcacctt tctgctcgca gttgcacgag ttctagccat
     2521 gcaacgggtc gcaatataac tgagtcaggc tggagcggcc gtgagcggtc tgctggggga
     2581 ggccgcgcct accggggagc ccgtctctgt gtcggaaggc tgcggtaacg ttagccacca
     2641 agtagcaagg cgcgccgcgc tcgaccttcc gccttctcaa gcgcaaaggg gcgcgacgca
     2701 cgctaggagc gagcatgcca aacgggtccg aacagcccac cccaaccgcc gccgctaccg
     2761 cggcccccga gacgggcgcg cgcacgccga aggacaacct cgtcgtgacg cgccacgaga
     2821 cgaccatcct cggcaagaag gtctcgtaca ccgccaccac gggcacgatc gtgctcagcg
     2881 aggaggccgt cggagagggg gagaaggccg gcaagttcga gggcgtgaag ccgcgcgccg
     2941 aagtcttctt cgtggcctac gagttggacg gcgttacgga caagagcaag cggccggtca
     3001 cgttcagctt caacggcggc ccgggctcgt cttccgtctg gatgcacctc ggcctcttcg
     3061 ggcccaagcg cgtcgagctc gacgacgacg gctacccctc cgcgccgccc taccggctgg
     3121 tgcagaacga cgtatcgctc ctcgacgtca ccgacctcgt gttcatcgac cctgtgagca
     3181 ccggctacag ccggccggtc gagggcgtga aggcgaagga cttccacaac gtggacgccg
     3241 acgtggagag cgtcggcgcg ttcatccgcc tgtacacggg ccggtacgga cggtggacga
     3301 gcccgaagta cctcgcaggc gagagttacg gcacgacgcg cgccgccggt ctggccggcc
     3361 acctgcagga gcggcacggc ctatacctga acggcctcat cctcgtgtct gccatcctcg
     3421 acttctccac ggcccgcttc ggccctggca acgacctgcc gccgctcatg ttcctgccga
     3481 cgtacgccgc cgccgcccac tatcacggtc agctcgacgc ggagctgcag gcgctgccgc
     3541 tgtccgtgtt gctcgagcgc gtcgaggtgt tcgtccatga gcgctacgcg cccgc
//
LOCUS       DAOQYE010000084         3585 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_27040_length_3585_cov_3.036261, whole genome shotgun sequence.
ACCESSION   DAOQYE010000084 DAOQYE010000000
VERSION     DAOQYE010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 3585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3585
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27040_length_3585_cov_3.036261"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            217..612
                     /locus_tag="PKN52_02315"
     CDS             217..612
                     /locus_tag="PKN52_02315"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HNQ98805.1"
                     /translation="MDTGLACSLLGIRKPEHLRDHSLRGPLFETFVASELLKWYLHRG
                     LPADLHRYREVRGAEVDLVRPGTPRRISLETKAGQTFQPELLKRLGGLDQEAWDSYLV
                     YGGVTRQTREGVQVVPWHDLASADWSSGR"
     gene            complement(630..2009)
                     /locus_tag="PKN52_02320"
     CDS             complement(630..2009)
                     /locus_tag="PKN52_02320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNQ98806.1"
                     /translation="MPQRTETAGRDPDPLFPSVGRLVRFGVPVTLAGIAVPLMSLVDV
                     AVLGRLGDPAVIAGVGVAGTILTTVMWCFSFLRFTTTGLVAQAMGRDDRRGVVLQGLR
                     PMVAALVGGIALWLLQGPIIALGLTLIAPEPEVAAFSRAYFQVRVWGAPLTLTITTLS
                     AWFMGLGAGRTVMLVQLFLNGLNASLTLTFVLGLGWGVAGAAAGTVLAELATVVLIVV
                     LALRRLPLQLWRQPPTAIFERGAWRQLFAANADIVVRTLLLTVCLALITERGARFGTL
                     TLAADQVLMQTFLLIANLLDGVAIAAEVFVGRAVGAANVAALRHVVGRSAMLALAWGG
                     LIALLVAPAAGVYLPLMTVDPALVAETRRYWPWLALLPLVSVWAFLVDGIYFGSLRTW
                     VLRNAMLAGALLYVAGLLVFGPLLGNHGLWLSLALLMMARAVGGVIAWSRLARDVANG
                     RGGRLGRRA"
     gene            2135..2977
                     /locus_tag="PKN52_02325"
     CDS             2135..2977
                     /locus_tag="PKN52_02325"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HNQ98807.1"
                     /translation="MTTALGTTTSIATSIATDTRANIAMSMSTSTNATTESAGGRVAP
                     AGRSTLRLAAMLGLTLAALSWLTAPARAQSAPAGITVTGTGTVYGEPDMATVELGFVA
                     VAADVRQALTEADAVLGAIQQAVVGAGVQAADVRTTGFYVWRDQTYDRDGNPGEVRFQ
                     VRHAYQVIVRDVAKLGDVISAAVDAGANEVGGIQMSVSDPTALASAARELAMADARAK
                     AAELADLGGVGLGAPIAIGEFGSGSPVSSAGRVAYDSMSAMGSVEVGQLAVTVQVQVT
                     FAID"
     gene            complement(2996..>3585)
                     /locus_tag="PKN52_02330"
     CDS             complement(2996..>3585)
                     /locus_tag="PKN52_02330"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNQ98808.1"
                     /translation="IPPDSGTISFGGVAHDELGPEGIAARTSAVFQSIVLVGDTLLEN
                     VAAGRPGATVAECDEVARAAQLSELVGRLPRGWLTPVGEDGGQLSGGERQRVTLARAL
                     LKDAPVLLLDEATSSLDTLTEAGFMSALAARRGKQACVIVAHRLNTIASADRIVFIEG
                     GAVREAGTLDELLALGGRFAAYWQQRQVAGQWALG"
BASE COUNT          607 a         1263 c         1236 g          479 t
ORIGIN      
        1 tgcctcgttg catatcgtgt cgtttttcaa actagacctc ccatgctcgc cgtgccgacc
       61 caggcggcag cgtcttggaa ggaagtcgag ttcgatcatc gcgtagtagg aggctggtga
      121 ttcgtcctcg atcgtgtccc aggcgtgcac gacttctgac gccgtgccat caagagtgaa
      181 cagaccctag tcggcgccgc cgacgccgga ttggacgtgg acactgggtt ggcctgctcg
      241 ctcttgggta tccgcaagcc tgagcatctc cgcgaccact ctttgcgtgg tccactcttc
      301 gagacgttcg tggcgagcga actcctgaag tggtacctgc accgtggatt acccgccgat
      361 ctacatcgct atcgcgaggt caggggcgcc gaggtcgacc tcgtcaggcc cggaacgccc
      421 cgcaggatct cgctcgaaac caaggccggc cagacctttc aaccggagct tctcaagcgc
      481 ctaggtggtc tggatcagga ggcgtgggac tcttacctcg tctacggcgg cgtgacgagg
      541 cagaccaggg aaggcgtgca ggtcgtgccg tggcacgacc ttgcgagcgc cgactggagt
      601 tccggtcgat gattcccaag caacacccat cacgcgcgcc gtccaagccg cccaccccga
      661 ccgttcgcca cgtcccgcgc caaccgcgac cacgcgatga ccccgccgac ggcccgcgcc
      721 atcatcagga gcgctagcga caaccacaac ccgtggttgc cgagcaacgg gccgaacacg
      781 agcagccccg cgacgtaaag cagggctccc gcgagcatgg cgttgcgcag cacccaagtg
      841 cgcagcgagc cgaagtagat gccgtcgaca aggaaggccc agacgctgac cagcggcaag
      901 agcgccagcc agggccagta gcgccgcgtc tccgcgacca gtgcggggtc gaccgtcatg
      961 agcggcaggt acacgccggc ggccggcgct accagcaggg cgatgagtcc gccccatgcg
     1021 agggcgagca tcgcgctgcg gccgacgacg tggcgcaggg cggcgacgtt ggcggcgccg
     1081 acggcgcggc cgacgaagac ctcggcggcg atggctaccc cgtcgagcag gttggcgatc
     1141 agcaggaacg tctgcatcag cacctgatcg gcggccaggg tcagggtgcc gaagcgggcg
     1201 ccgcgttccg tgatcagggc gaggcagacg gtgaggagga gggtcctcac gactatgtct
     1261 gcgttcgccg cgaagagctg ccgccacgcg ccccgctcga agatcgcggt cggaggttgg
     1321 cgccacagct gcagcggcag gcggcggagc gccagcacta cgatcaggac gacggtggcc
     1381 agctccgcca gcaccgttcc ggcggcggcg ccggcaacac cccagccgag gccgaggacg
     1441 aaggtaagcg tcagcgacgc gttgaggccg ttgaggaaga gctgcacgag catgacggtc
     1501 cgcccggcgc cgaggcccat gaaccaggcc gacagcgtcg tgatggtgag ggtgagcgga
     1561 gcgccccaca cgcgcacctg gaagtacgcc cggctgaagg ccgccacctc gggttccggc
     1621 gcgatgagcg tgagcccaag cgcgatgatc gggccttgca gcagccacag cgcgatgcca
     1681 ccgaccaagg ccgccaccat gggccgcagc ccctgcagca ccacgccccg ccggtcgtca
     1741 cggcccatgg cctgcgccac caggccggtg gtcgtgaagc ggaggaagct gaagcaccac
     1801 atgacggtcg tgaggatcgt gccggccacg ccgacgccgg cgatcacggc cgggtcgccg
     1861 agcctcccga gcaccgcgac gtccacgagg ctcatgagcg gcaccgcgat gccggccaaa
     1921 gtgaccggga cgccgaagcg caccaggcgc ccaacgctgg ggaacagtgg gtcggggtcg
     1981 cgccccgcgg tctccgtacg ttggggcacg ccgcatccta tcgccgtgga ccgagcgcgc
     2041 gggagtcgtg aacgctgggt gaaccgatct cacccgaggc tcgccgacct catggtcgcg
     2101 gcggcgaaca tcggggcaag gagtgtgact ctctatgacc accgccctgg gcacgaccac
     2161 cagcatcgcc accagcatcg ccaccgacac ccgcgcgaac atcgccatga gcatgagcac
     2221 gagcacgaac gcgaccacgg agtccgccgg gggccgtgtc gcccctgccg gccgttctac
     2281 cctccgccta gccgcgatgc taggtctgac tctagcggcc ctgtcgtggc tgaccgcccc
     2341 ggcacgggcc cagtcggccc cggccggcat aacggtcacg ggcaccggca ccgtctacgg
     2401 cgagccggac atggccaccg tagaactggg cttcgtggcg gtggcggccg acgtgcgtca
     2461 ggccctcacc gaggcggacg cggtgttggg cgccatccag caagccgtcg tcggcgcggg
     2521 ggtgcaggcc gccgacgtgc gcaccaccgg cttctacgtc tggcgcgatc agacgtacga
     2581 ccgcgacggc aacccgggcg aggtgcggtt ccaggtgcgc cacgcgtacc aggtgatcgt
     2641 gcgagacgtg gccaagctcg gcgacgtgat ctcggcggcg gtcgatgccg gcgccaacga
     2701 ggtcggcggg atccagatgt ccgtgtccga cccgaccgca ctggccagcg cggcgcgcga
     2761 actcgcgatg gccgacgccc gcgccaaagc cgccgagctc gccgacctgg gcggtgtcgg
     2821 tctcggcgcg cccatagcga tcggtgagtt cggctcgggc tcgcccgtgt cgagcgccgg
     2881 cagagtggcc tatgactcca tgagcgccat gggaagcgtt gaggttggcc agttggccgt
     2941 gaccgtgcag gtgcaagtca ctttcgcgat cgactgaggg ccgggcgccg ggtcgtcagc
     3001 ccaacgccca ctgccccgcc acctgccgct gctgccaata agcggcgaac cgacccccga
     3061 gcgcgagcag ctcgtccaac gtgccagcct cccgaacggc gccgccctcg atgaagacga
     3121 tgcgatcggc gctagcgatc gtgttgaggc ggtgcgccac gatgacgcag gcctgcttgc
     3181 cgcgccgagc cgcgagggcc gacatgaagc cggcttcggt gagcgtgtcg agcgagctgg
     3241 tcgcctcgtc caggagcaac acgggcgcgt ccttgagcag cgccctcgcc agggtcactc
     3301 gctggcgctc gccaccagag agctgcccgc cgtcctcccc caccggcgtc agccagccgc
     3361 gcggcaggcg cccgacgagc tcgctcaact gcgcggcccg agccacctcg tcgcactcgg
     3421 cgaccgtggc cccgggtcgg ccggccgcga cgttctccaa cagcgtgtct ccgacgagca
     3481 cgatcgattg gaagacggcc gaggtccttg cggcgatccc ctcggggccg agctcgtcgt
     3541 gagcgacgcc cccgaagctg atcgttccgg agtccggcgg gatga
//
LOCUS       DAOQYE010000085         3584 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_27049_length_3584_cov_3.679229, whole genome shotgun sequence.
ACCESSION   DAOQYE010000085 DAOQYE010000000
VERSION     DAOQYE010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 3584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3584
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27049_length_3584_cov_3.679229"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1120)
                     /locus_tag="PKN52_02335"
     CDS             complement(<1..1120)
                     /locus_tag="PKN52_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNQ98809.1"
                     /translation="MRRLITAAMVALLAAIGTASAQTIVRVQGYGGQDPAIVQRLIDE
                     VIGADLAAQGITINYEPIETDYNTVLVNSLSAGTAGDVFYVPGETAPGFIATGKLLAL
                     EDRVDTSAFIDTLNAVFSKDGHVYGIAKDFNTLAVFYNKDLFDAAGVAYPNADDDWNT
                     FADKLAKVATLDPGVYGACIAADTARLGAFAFANGWTTFTADGGVDLSSPAFAEAFDF
                     YTGLVTSGAAAQPADLGAGWPGDCLAREQAAVAIEGAWILGFLRDNAPNLQYGATFLP
                     KSPTTGRSGNYLYTVAWAVNADSKVQDAAVKVLEALTSPAAQQFILEQGLAIPSRKEL
                     ADNPYFSEDSAEAQANKVVFEGASHGNVYGFQFGTIGTD"
     gene            complement(1242..2228)
                     /locus_tag="PKN52_02340"
     CDS             complement(1242..2228)
                     /locus_tag="PKN52_02340"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNQ98810.1"
                     /translation="MSERATIQQVAEHAGVSIGTVSRVLNSRAGVSQATRERVRASIR
                     ALAFRPDHVARALSKAPLRVGLSLASGTRRLTPFFMLFLEHLIARLQQDGYRFEEVPA
                     GRDGLPEWLPNGVILHGAHEDDPRLAYLRDQGVPFVLVGRAPGVRWVAPDDHDGGLQA
                     TRHLLALGHREILHLGGLMGHQAFQDRYGGYLQALDEAGIGPRRDWLLDGGFTTLGGY
                     RAVRRAFESGCACTAVFAASDEMALGAIAALEDLGLTVPLDVSVVGFDDLPEVADALR
                     IPEGLTSVRQDIAHITATAVTLLQEGLNGAPIRSETVPVQLMARGTTARRRR"
     gene            2926..>3584
                     /locus_tag="PKN52_02345"
     rRNA            2926..>3584
                     /locus_tag="PKN52_02345"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          611 a         1079 c         1295 g          599 t
ORIGIN      
        1 agtcggtgcc gatggtgccg aactggaacc cgtacacgtt gccgtgcgag gcgccttcga
       61 agacgacctt gttggcctgc gcctcggcgg agtcctcgct gaagtacggg ttgtcggcca
      121 gctccttgcg gctggggatg gcgaggccct gctcgaggat gaactgctgc gccgcggggc
      181 tcgtgagcgc ctccaggacc ttcacggcgg cgtcttgcac cttgctgtcg gcgttgacgg
      241 cccaggcgac cgtgtagagg tagttgccgc tccgacccgt cgtcggcgac ttcggcaaga
      301 aggtcgcgcc gtactgcagg ttgggagcgt tgtcgcgcag gaagcccagg atccaggccc
      361 cttcgatcgc gacggcggcc tgctcgcgcg cgaggcagtc gccaggccag ccggcgccga
      421 ggtcggcggg ctgggcggcc gcaccgctcg tcacaaggcc cgtgtagaag tcgaacgcct
      481 cggcgaacgc cggggaactg agatcgacgc caccgtcggc cgtgaacgtc gtccagccgt
      541 tggcgaaggc gaacgcgccg aggcgcgccg tgtcggccgc gatgcaggcg ccgtacacgc
      601 cgggatccag cgtggcgacc ttggcgagct tgtcggcgaa ggtgttccag tcgtcgtcgg
      661 cgttcgggta cgccacgccg gcggcgtcga acaggtcctt gttgtagaag acggcgaggg
      721 tgttgaagtc cttggcgatg ccgtagacgt gaccgtcctt cgagaagacc gcgttcaacg
      781 tgtcgatgaa cgcgctcgtg tcgacgcggt cctcgagcgc gaggagcttg cccgtggcga
      841 tgaagccggg cgccgtctcg ccgggcacgt agaagacgtc gccggcggtg ccggccgaga
      901 gcgagttgac gaggaccgtg ttgtagtcgg tctcgatggg ctcgtagttg atggtgatgc
      961 cttgggcggc caggtcggcc ccgatgacct cgtcgatgag gcgttggact atggccggat
     1021 cctgtccgcc gtagccctgc acccgcacga tggtctgggc gcttgccgta ccgatggcgg
     1081 cgagcagggc caccatcgcg gctgtgatga gtcgtctcat gtttcctcct aggcgttcct
     1141 tgtcggcgtc gtgggccagg gccctagaca tccgcgtgac cggtgagggt ggatgcggtt
     1201 tggcgaccta gagcgcttgc cgccgctggt tcttcaggcg ctcacctcct tcttcgcgcc
     1261 gtcgtccccc gagccatgag ctgcacgggg acggtctcgg accggatggg cgcaccgttc
     1321 aggccctcct gcaggagggt gacggcggtg gcggtgatgt gggcgatgtc ctggcggacc
     1381 gaggtgaggc cttccgggat cctgagcgcg tcggccacct cgggcaggtc gtcgaaaccg
     1441 actacggaga cgtcgagggg aacggtcagc cccaggtcct cgagggctgc gatggcgcca
     1501 agggccatct cgtctgaggc ggcgaagacg gccgtgcagg cgcaaccgct ctcgaacgcg
     1561 cggcgcacgg cccggtagcc acccagggtg gtgaagccgc cgtcgagcag ccagtcgcgt
     1621 cgggggccga tgccggcctc gtcgagcgct tggaggtagc cgccgtagcg gtcctggaag
     1681 gcctggtggc ccatgaggcc gccgaggtga aggatctcgc ggtggccgag cgccaggagg
     1741 tggcgggtgg cctgcaggcc gccgtcgtgg tcgtcgggcg ccacccacct caccccgggg
     1801 gcgcggccca ccaggacgaa gggcacgcct tggtcgcgca gataggcgag gcgcgggtcg
     1861 tcctcgtggg cgccgtgcag gatcacgccg ttcggcagcc actccgggag gccgtctcgg
     1921 ccggcgggca cctcctcgaa gcggtagccg tcctgctgca accgggcgat caggtgctcg
     1981 aggaagagca tgaagaaggg ggtgaggcgg cgcgtgccgc tcgcgaggct gaggccgacc
     2041 cggagcggcg ccttgctgag cgcccgcgcg acgtggtcgg ggcggaaggc gagcgcgcgg
     2101 atgctggcgc gcacgcgctc gcgcgtcgcc tggctcaccc ccgcgcggct gttcagtacc
     2161 cgcgagaccg tgccgatgct gacgcccgca tgctcggcga cctgctggat ggtggcgcgt
     2221 tcgctcactt caagctcctg acgggtccgc gtgcctgtcg gctcgcctcg cgctcgcacg
     2281 gttgatggcc ctcgcgccgc tggtacgccc tcggtcgccg atcctccgta agcgcttacg
     2341 tactcgcttt gaagagagga taggggcagg accggtttct gtcaacgaca accgtacgtg
     2401 ttcgaacgat cgatggatcg cgcaaccagc gtcgagccta cgactacttt cgtaagcgct
     2461 tacggaaagt aaggccgagc gagtcgagcg aagtgccgga ggcgcgccgg cgggggtgac
     2521 gcgcgcgtcc gcggggagcg cccgaggacg atgagggtgc gaccgccacg ttcttcgagc
     2581 ttcgctgccg gtaacgcggc gggggaggtg gccggcccgg cagagccgct gccggctcgc
     2641 gcgccctcgc gcgtacggtt ttcggtgagg cctttcggtg aggccgtcct cggatctttc
     2701 ggtgatcgac taggggggtg ttgacacgcc gcgccctgga gatgtactct aacttttgcc
     2761 gctcgaaaga ccggcgcgaa cattgacagg cgaatagaag gcaagacatc agggctcgga
     2821 cgggctcgtt gttccgtcag gaacacgaaa acgtacgggc cgcgaatcgg gttttttcga
     2881 aaacttgaat cgcgtgtctg ctcatacaca tcaatgaaag aaatgggtca agttactaag
     2941 ggcacacggt ggatgccttg gcagtcgaac cgatgaagga cgtggctacc tgcgataagc
     3001 ctcgggaagc tggaagcaag cgtagatccg aggatttccg aatggggcaa cccagtgggg
     3061 cgatccccca ctacccatga ctgaatacat aggtcatgtg gagggaaccc agggaactga
     3121 aacatctaag tacctggagg agaagaaaga aacctcgatt cccccagtag cggcgagcga
     3181 aaagggaata gcctaaaccg gctcctacgg gagccggggt tgtaggaccg gcaacatgga
     3241 ctcgagcgta tagcggaagc gcactggaaa ggcgcgccat agcgggtgat agccccgtac
     3301 gcgaaatgcg cggaagccta gccggcacct gagtagcgcg agtcacgtgg aacctcgcgt
     3361 gaatccacgg ggaccacccc gtaaggctaa atattccgac tgaccgatag cgaacaagta
     3421 ccgtgaggga aaggtgaaaa gaaccccgtg aggggagtga aatagtccct gaaaccgtgt
     3481 gcttacaagc agtcacagcc ctatgcgcac cttcgggtgt gaacgggtgg tggcgtgcct
     3541 cttgaagcat gatcctgcga cttactggta gtgccgagtt taag
//
LOCUS       DAOQYE010000086         3581 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_27079_length_3581_cov_2.584515, whole genome shotgun sequence.
ACCESSION   DAOQYE010000086 DAOQYE010000000
VERSION     DAOQYE010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 3581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3581
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27079_length_3581_cov_2.584515"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="PKN52_02350"
     CDS             <1..526
                     /locus_tag="PKN52_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98811.1"
                     /translation="PTTPSGQIALYDGTITARSINDGGQGAFVFVDPAAGSGARVAGA
                     AGVDRGTLYLRADVSAKPSSTPMQLQLCLVPGDDSVISPPCTDATKLVVTGTGAFSAS
                     FDMRALTGEESVDWTRGLSRLLVVLRDSASRPLDERYTRGADGTPIDLTPYYPVTLRL
                     RLVLAPAGATFSGW"
     gene            complement(582..1937)
                     /locus_tag="PKN52_02355"
     CDS             complement(582..1937)
                     /locus_tag="PKN52_02355"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HNQ98812.1"
                     /translation="MRVTIIGTGYVGLTTGTALAYLGNEVTFVDKKREVIEQLAAGIP
                     TIHENGLAEIMAAGRQHATFRTDIPELSGEGVVLIAVGTPSREDGDADLSFVDVAATE
                     VADKVQPGARLVVVNKSTVPVGSARHVEGIITRRLAARGVSAEVHVASNPEFLAEGRA
                     VQDTLYPDRIVVGSDTQAARAMLRELYAPILEQTFDPPPGSPRPERLELPPFIGTTTT
                     SAELTKYAANAFLATKISFINEFSVLAEKVGADIVEVARAIGLDSRIGGKFLNAGIGW
                     GGSCFGKDTAAVMALGNSYDYQMPLVRAAVDVNKRQRMYVVEKLQQHLKVVRGTTIGL
                     LGLAFKANTDDMRDAPALTLVRELSQRGAVLRVHDPIAMDNAKRHFPDLPVEYCASEV
                     EMADGCDAIVVVTEWPQYRQVDFAALARVMRGDLLLDARNLLQPAAVTAAGLNYVGIG
                     R"
     gene            complement(2055..2609)
                     /locus_tag="PKN52_02360"
     CDS             complement(2055..2609)
                     /locus_tag="PKN52_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HNQ98813.1"
                     /translation="MQTADTRGPKPAPGEAPEQRVALYVDTQNLYYAARDGFDASVDY
                     QTLLDAALRGRRLAHANAYVVERDGDSTAHGFFGKLSSLGYRVKRRKVRVHRVDDDGA
                     VRLEGDWDMGIAADIVRALDHADVIVLASGDGDFAPILELAQERGKRVEVMAFREAAG
                     QSLQDMADRFVALGEVPGIFLAGR"
     gene            complement(2668..3168)
                     /locus_tag="PKN52_02365"
     CDS             complement(2668..3168)
                     /locus_tag="PKN52_02365"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNQ98814.1"
                     /translation="MLVRDAIPADAEAVRGLLAGVYREGGAFVGDGAESVGSLASRIG
                     TAPRRSHYAVAVLSGVIVGWAELQRSPARRLEHVAVLTLAVAPGARRRGVGRAVLREC
                     YDWCGRVGVLKMSLNVRAGNLAAIGLYESEGFEHEGREVRQIRLLGAEGDGFEDNLIM
                     GKWLAR"
     gene            3252..>3581
                     /locus_tag="PKN52_02370"
     CDS             3252..>3581
                     /locus_tag="PKN52_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HNQ98815.1"
                     /translation="MSRLVSAESVAEGHPDKLADRISDSVLDAILAQDPQARVAVEAL
                     LTRGLALVAGEVTTKADVDVQAIVRSAAREVGYIDAAYGFDADHSAVLIALNEQSPDI
                     AQGVDRAD"
BASE COUNT          527 a         1280 c         1211 g          563 t
ORIGIN      
        1 tccgaccacg ccttccggtc agatcgccct gtacgacggc accatcacgg ccaggagcat
       61 caacgacggc ggccagggcg ctttcgtgtt cgtggatccg gcggccggct ccggcgcgcg
      121 cgtcgccggc gcagccgggg tggaccgcgg caccctgtac ttacgggccg acgtgagcgc
      181 caagccgagc tcgacgccga tgcagcttca gctctgcctg gtgcctggcg acgactcggt
      241 catcagcccg ccctgcaccg acgccacgaa gctggtcgtg acgggcacgg gcgcattcag
      301 cgccagcttc gacatgcgcg cgctcaccgg cgaggagagc gtcgactgga cgcgaggact
      361 cagccggctg ctcgtcgtgc tgcgcgattc ggcgtcgcgc ccactggacg agcgctacac
      421 gcggggcgct gacggcacgc cgatcgatct gacgccctac taccccgtga cgctgcgctt
      481 gcggctcgtg ctggcgcctg ccggagccac gttctccggt tggtgagcgg ccggcgcccc
      541 atcacggcag cggccgggac cgtcgcctcg cgagcccgac gctaccgccc gatcccgacg
      601 tagttgagcc cggcggccgt gacggcggcc ggctgcagga ggttacgcgc gtcgagcagg
      661 aggtcgccgc gcatcacccg cgccaaagcc gcgaagtcga cctgccggta ctgcggccac
      721 tccgtgacga ccacgatggc gtcgcaaccg tcggccatct ccacctcgga agcgcagtat
      781 tcgactggca ggtcagggaa gtggcgcttg gcgttgtcca tggcgatggg gtcgtgtacc
      841 cgcaggacgg cgccgcgctg gctgagctcg cggacgagcg tgagggcggg ggcgtcgcgc
      901 atgtcgtccg tgttggcctt gaacgccagg ccgagcagcc cgatcgttgt gccccgcacg
      961 accttcaggt gctgttgcag cttctctacg acgtacatgc gctgacgctt gttgacgtcg
     1021 acggccgcac gcacgagcgg catctggtag tcgtaagagt tgccgagcgc catcaccgcg
     1081 gcggtgtcct ttccgaagca gctgccgccc cagccgatgc ctgcgttgag gaacttgccg
     1141 ccgatccgac tgtcgaggcc gatggcgcga gccacctcga cgatgtcggc ccccaccttc
     1201 tcggccagga ccgagaactc gttgatgaag ctgatcttgg tggcgaggaa cgcgttggcc
     1261 gcgtacttgg tgagctccgc gctggtggtg gtcgtcccga tgaagggcgg cagttccagc
     1321 cgttccggcc gcggggagcc gggaggcggg tcgaaggtct gctcgaggat gggtgcgtac
     1381 agctccctga gcatggcgcg cgccgcctgc gtgtcggaac ccactacgat ccggtcgggg
     1441 tacagggtgt cctgcacggc cctgccctca gcgaggaact cggggttgga cgccacgtgc
     1501 acctcggcgc tcacgccacg agccgccagc cgtctggtga tgatgccctc cacgtgacgc
     1561 gcgctgccga ccggtacggt ggacttgttg accacgacga gcctggcgcc cggctggacc
     1621 ttgtcggcga cctcggtcgc cgcgacgtcc acgaacgaca ggtcggcgtc gccatcctcc
     1681 ctggacgggg tgccgaccgc gatgagcacg accccctcgc cgctcagttc ggggatgtcc
     1741 gtccggaacg tcgcatgctg cctgcccgcc gccatgatct cggcgagccc gttctcgtgg
     1801 atggtcggta tgccggcggc gagctgctcg atgacctcgc gcttcttgtc gacgaacgta
     1861 acttcgttgc ccaggtaggc gagggcggtg cccgtcgtga gtccgacgta acccgtgccg
     1921 atgatggtca ctctcatgcg tccatcctac gcatgcgcgc ctaaaagcat tcttactgac
     1981 gaggtccggt tacccgccca ggcccgcagg ggggagctca ccgcggtggc aacagccgaa
     2041 cactcctgtg gcgttcagcg ccccgcgagg aagatccccg gcacctcgcc gagcgcgacg
     2101 aaccggtcgg ccatgtcctg cagcgactgg ccggccgcct cgcggaacgc catgacctcc
     2161 acgcgcttcc cgcgctcctg ggcgagttcg aggatgggcg cgaagtcgcc gtcgccgctg
     2221 gcgagcacga tgacgtcggc gtgatcgaga gccctgacga tgtcggccgc gatgcccatg
     2281 tcccagtcac cctcgaggcg cacggcgccg tcgtcgtcca cacggtggac ccgcaccttg
     2341 cggcgcttga cccggtagcc gaggctgctc agcttgccga agaaaccgtg ggccgtgctg
     2401 tcgccatcgc gctcgaccac gtaggcgttg gcgtgcgcca ggcgtctccc gcgcagagcc
     2461 gcgtcgagca gcgtctggta gtcgacgctt gcgtcgaaac cgtcgcgggc cgcgtagtag
     2521 aggttctgcg tgtcgacgta gagagccacc cgttgctcgg gcgcctcgcc gggcgccggc
     2581 ttcgggcccc gcgtatcggc cgtctgcatg ggcgcagtgt aacgtttacg cgagagcgcg
     2641 ttaccccggc ggggaggccc tgcgaggcta gcgggcgagc cacttcccca tgatcaggtt
     2701 gtcctcgaac ccatcacctt cagcgccgag cagccttatc tggcgaacct ccctgccctc
     2761 atgctcgaac ccctcgctct cgtatagccc gatggcggcg aggttgccgg ccctgacgtt
     2821 caaagacatc ttgaggaccc cgacccggcc gcaccagtcg tagcattcgc gcagcacggc
     2881 ccgtccgaca ccgcggcgcc tggcgccggg cgcgaccgcc aaggtcagga ccgccacgtg
     2941 ctccaggcgc ctggccggcg agcgctgcaa ctcggcccaa ccgacgatga cgccgctcag
     3001 caccgccacg gcgtagtggc tacggcgcgg cgccgtccct atccggctgg ccagggaacc
     3061 gacgctctcg gcgccgtcgc ccacgaaggc cccgccttcg cggtagacgc cggccaacag
     3121 ccccctgacg gcctcggcgt cggccgggat cgcatccctg acgagcacgc gccgatgata
     3181 accgcgccgt aggcctggta tcctggcttg gttgtcgcct cggcgacgtt cccatggagg
     3241 tcccgacccg catgtcccgc ctagtgagtg cagagtccgt ggcggaaggc caccctgaca
     3301 agttggcaga ccgcatcagc gacagcgtgc tggacgccat cctcgcccag gacccgcagg
     3361 cgcgggtcgc cgtggaggcg ctcctgacgc gtgggctcgc cctcgtcgcg ggcgaggtca
     3421 ccaccaaggc cgacgtcgac gtgcaggcca tcgtgcgctc cgcggcgcgg gaggtcggat
     3481 acatcgacgc cgcctacggc ttcgatgccg accacagcgc cgtcctcatc gcgctcaacg
     3541 agcaatcgcc cgacatcgcc caaggcgttg atagggccga c
//
LOCUS       DAOQYE010000087         3564 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_27274_length_3564_cov_4.108293, whole genome shotgun sequence.
ACCESSION   DAOQYE010000087 DAOQYE010000000
VERSION     DAOQYE010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 3564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3564
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27274_length_3564_cov_4.108293"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1099
                     /locus_tag="PKN52_02375"
     CDS             <1..1099
                     /locus_tag="PKN52_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98816.1"
                     /translation="GLPLWAKIVPMLLILLVGAYLAGRGLRPRLFGFTAALAAFSGLA
                     TYVPFLKSGYHLMVVGSKALPISMMVSGANGIAWYLFVAVYLVATWRVRRGPVLRLLD
                     GAVAMIVVSTVAIAALAYLGMSGQVARPMMLALVDWFLTCFADGWFGLAVLGLAARHA
                     PSARLSRSPVGLLTWSLVAAIAVRSAARFATDGLGWQWPAGFDAVASALAALAWLLLV
                     RGAWPVDAKAGLSGSTLWLRQLALALLALKGTVELAGALPPVRAELMAAPFHVLFLHA
                     FLLGAVSFSLLYGARTTIGPGAFKGAGAFVVAVGGMVGALVTLTPVWPSALSGPWVLY
                     ATALTSLGPLLVALQALVRLGPWWRPTTTRP"
     gene            complement(1056..2213)
                     /locus_tag="PKN52_02380"
     CDS             complement(1056..2213)
                     /locus_tag="PKN52_02380"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNQ98817.1"
                     /translation="MSSQPAALKPLIAKPVTRRAVLWAAIAAFFLIGAVQALYGPAFP
                     LLRERFGIGLEQVSIVVSAQFLGAFAGIVLSGFFLRALGYRRVLLAACVALALGVGVV
                     AVAPVWLAVLGGAVLAGIGAGLLNVTGNLLIAVAFRPTAAPALNIINAAFGVGAVVGP
                     LLVTVTEPHYGWAFAAAALVAILLLPWFTRLSVPDAAPPERGAAPVAWGSLAAFVLLY
                     VFYVSAEVGVTSWETEYLTPSFGTRAAAFTSLYWLAITAGRLLAAPLSARIRTHHFVL
                     GATVATLVFMVAAHRVESAPIAFVLVGLSLAPIFPTALAWLTEVFPQRAEQVTPVVVA
                     AANLGPALSSPVIGAVIHAGGVQVVPTALSAMALALLLTVVWLWVATKGRA"
     gene            2314..>3564
                     /gene="alaS"
                     /locus_tag="PKN52_02385"
     CDS             2314..>3564
                     /gene="alaS"
                     /locus_tag="PKN52_02385"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HNQ98818.1"
                     /translation="MVNGIQQINELREKYLRFFQARGHLIHPSASLKSDDPTLMFTSA
                     GMVQFKPYFLGATPKFAGVDGTWHRVTTAQKCLRINDIENVGRTLRHHSFFEMMGNFS
                     FGDYFKAEAAAWAWEFATSSEYLGLDPERLYVTVYQDDDEAFRIWNEQVGVPAERISR
                     WGEDDNFWPANAVSKGPNGPCGPCSEIFYDRGPDFGTVGESGPNTGSGDRYIEFWNLV
                     FTQFDRRDGGHLEPLPQKNIDTGLGFERLAAVMTGLEDAYATELFQPTIRRVAALSGV
                     PYEGKASVSHRVIADHLRAVTFAITDGVLPANDGAGYVVKMLVRRASRHAWLLGLREP
                     VLHSLVDQVVEAMGEPYPELKDGAERVKGIIHAEEDQFLRTLEAGIHRVGVLLDELRG
                     SVLPGDVAFDLWQTYGFPLDLTSEM"
BASE COUNT          543 a         1248 c         1249 g          524 t
ORIGIN      
        1 cgggcttccc ctctgggcca aaatcgtgcc gatgctgctg atcctgctcg tcggcgccta
       61 cctcgccggc aggggcctgc ggccccgcct cttcggcttc acggccgcgt tggccgcctt
      121 cagcgggctg gcgacctacg tcccgttcct gaagagcggc taccacctca tggtggtcgg
      181 ttcgaaggcc ctaccgatct cgatgatggt ctccggtgcc aacggcatcg cctggtacct
      241 cttcgtggcc gtctacctgg tcgccacgtg gcgcgtgagg cgcgggccgg tgctgcggct
      301 gctcgacggc gccgtggcca tgatcgtcgt cagcacggtc gccatcgccg ccctcgccta
      361 cctcggcatg agcggacagg tcgcgcggcc catgatgctc gcgctggtgg actggttcct
      421 gacctgcttc gcggacggtt ggttcggtct cgccgtcctg ggcctggcgg ccaggcacgc
      481 gccttccgcg cggctgagcc gatcccccgt cggcctgttg acctggtcgc tcgtggccgc
      541 catcgccgtg cgcagcgccg cccgtttcgc caccgacggt ctcggctggc agtggcctgc
      601 cggcttcgac gccgtcgcga gcgccctcgc ggcgctcgcc tggctgctgc tcgttcgggg
      661 cgcctggccg gtcgacgcca aagcggggct gagcggcagc accttatggc tgaggcagct
      721 cgccctcgcg ctgctggcgc tcaagggcac ggtcgagttg gccggcgcgc tgcccccggt
      781 gcgcgcggaa ctcatggccg cgcctttcca cgtcctcttc ttgcatgcgt tcctgctcgg
      841 agcggtgagc ttctcgcttc tgtacggggc ccgcacgacc atcggccccg gcgccttcaa
      901 gggcgccgga gccttcgtcg tcgcagtcgg cggcatggtc ggggcgctag tcacgctgac
      961 accggtctgg ccaagcgcgc tctccggtcc gtgggtactc tacgccacgg cgctaacgag
     1021 cctcggaccc ctgctcgtcg ccctgcaggc gctggtcagg ctcggccctt ggtggcgacc
     1081 cacaaccaca cgaccgtgag cagcagcgcc agggccatgg cggacagcgc cgtcggcacg
     1141 acctgtacgc cgcccgcatg aatgacggcg ccgatgacgg gcgagcttag ggccggtccg
     1201 aggttggcgg cggccaccac gaccggcgtg acctgctcgg cgcgctgcgg gaagacctcg
     1261 gtgagccacg ccaaagcggt cgggaagatg ggcgcgaggc tcaacccgac gagcacgaac
     1321 gcgatgggcg cgctctcgac gcggtgcgcg gccaccatga agacgagggt ggcgacggtc
     1381 gcgcccagca cgaagtggtg cgtccggatg cgggcggaca acggcgcggc cagcaggcgc
     1441 cctgccgtga tggccagcca gtagagcgaa gtgaaggccg cagcgcgcgt gccgaaactg
     1501 ggcgtgaggt actccgtctc ccacgacgtg acccctacct cggcggagac gtagaagacg
     1561 tagaggagca cgaacgcggc caggctgccc catgccacgg gagcggcgcc acgttctggt
     1621 ggcgccgcgt caggcacgct gagccgggtg aaccagggca gcaggaggat cgccacgagg
     1681 gcggccgcgg cgaaggccca gccgtaatgg ggctccgtta ccgtgacgag gagcggaccg
     1741 acgacggcgc caacgccgaa ggccgcgttg atgatgttga gagccggcgc cgccgtcggg
     1801 cggaacgcga ccgcgatgag caggttgcca gtcacgttga gcagcccggc cccgataccg
     1861 gccagcacgg cgccgccgag gacggccagc cacacggggg cgacggcgac cacgccgacg
     1921 ccgagggcga gcgcgacgca ggcggcgagg agcacgcggc ggtagccgag ggcccgtagg
     1981 aagaagcccg agaggacgat gcctgcgaag gccccgagga actgggcgct cacgacgatg
     2041 ctcacctgct cgaggccgat gccgaagcgc tcgcggagga gcgggaaggc cgggccatag
     2101 agcgcctgca ccgccccgat gaggaagaag gccgcgatgg cggcccagag tacggccctg
     2161 cgagtgacgg gcttggcgat gagtggcttg agtgccgcgg gctggctcga catgtccagc
     2221 gaggttaccg cgccggagaa cgccccgccc gccttccgat gatgccgggg cgggcgggta
     2281 tacttacccc tcggttactc ccggaggcac ctcgtggtga acggcatcca acagataaac
     2341 gaactgcgcg agaagtacct gcgcttcttc caggcgcgcg gccacctgat ccacccgtcc
     2401 gcctcgctca agagcgacga ccccaccctc atgttcacga gcgcgggcat ggtgcagttc
     2461 aagccgtact tcctcggcgc cacgcccaag ttcgcgggcg tcgatggcac gtggcaccgc
     2521 gtgaccacgg cgcagaagtg cctgcgcatc aacgatatcg agaacgtcgg gcgcacgctc
     2581 cgccaccact cgttcttcga gatgatgggc aacttctcgt tcggcgacta cttcaaggcc
     2641 gaggcggccg cctgggcgtg ggagttcgcg acctcgagcg agtacctcgg cctcgacccg
     2701 gagcggctgt acgtcaccgt gtaccaggac gacgacgagg cgttccgcat ctggaacgag
     2761 caggtcggcg tgccggccga gcgcatctcg cgctggggcg aggacgacaa cttctggccc
     2821 gccaacgccg tgagcaaggg cccgaacggt ccgtgcggac catgctccga gatcttctac
     2881 gaccgcggcc ccgacttcgg tacggtaggc gagtccgggc cgaacacggg ctcgggcgac
     2941 cgctacatag agttctggaa cctggtgttc acgcagttcg accggcgcga cggcggccac
     3001 ctggaacccc tgccccagaa gaacatcgac acgggcttgg gcttcgagcg cctagccgcg
     3061 gtcatgaccg ggctagagga cgcctacgcc accgagctct tccagccgac catccgccgc
     3121 gtcgcggccc tctcgggcgt gccgtacgag ggcaaggcgt cggtctcgca ccgcgtcatc
     3181 gccgatcacc tacgcgccgt gacgttcgcc atcaccgacg gcgtgctccc ggcgaacgac
     3241 ggcgcgggct acgtcgtgaa gatgctcgtc cgccgcgcct cgcgccacgc ttggctgctc
     3301 ggcttgcgtg agcccgtcct gcactcgctc gtcgaccagg tcgtcgaggc gatgggcgag
     3361 ccgtacccgg agctgaagga tggcgccgag cgtgtcaagg gcataatcca cgccgaggag
     3421 gaccagttcc tgcgaacgct cgaggctggc atccaccgcg tcggcgtgct cctggacgaa
     3481 ctgcgcggct ccgtcctgcc cggcgacgtg gcgttcgacc tctggcagac gtacggcttc
     3541 cccctcgacc tgacgagcga gatg
//
LOCUS       DAOQYE010000088         3542 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_27499_length_3542_cov_3.347003, whole genome shotgun sequence.
ACCESSION   DAOQYE010000088 DAOQYE010000000
VERSION     DAOQYE010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 3542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3542
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27499_length_3542_cov_3.347003"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="PKN52_02390"
     CDS             complement(<1..267)
                     /locus_tag="PKN52_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98819.1"
                     /translation="MKRSILIALMTLVALAGLANAQSDPSVTGTLVIYTTTYDIEYKL
                     IIEQGFQGKYPNVKVESVQAGAGELKTRIRSEAANPQADVMFGGL"
     gene            complement(264..1214)
                     /locus_tag="PKN52_02395"
     CDS             complement(264..1214)
                     /locus_tag="PKN52_02395"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HNQ98820.1"
                     /translation="MPELVGVGHICQDHLCVIEGYPPEDGSTRITEIEFQGGGAAATA
                     TVAAARLGIDTALIGNLGADRIGDEILDELRREGVDISALDRLEGVRSLSSYVMISPE
                     RGTRTKFPFKDRMPPIAWSERQLEALSGAKVLHLDGTHYENAVRAASLAKEHGVTVSL
                     DGSTRQSDNELNRRLASMADILITNAVYPRFVTGKGSLGEALLEMSTWGPRFVAATAG
                     QEGVYAVMGGEVRHYPAFRVKVVDTTGAGDVFHGAFLAARLRGKGVEECIRFAQYAAG
                     RKCEQMGGRKGIPDWATVETALSVLEADDTKNAPLKEVTR"
     gene            1608..3131
                     /locus_tag="PKN52_02400"
     CDS             1608..3131
                     /locus_tag="PKN52_02400"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNQ98821.1"
                     /translation="MKALNRLLAFAAALSLSAALAQGAPPQSSFVLGMSAETATFYPP
                     GTGGLFERNSFLQIFDSLMHREADGTVVPRIATAWEAVDATTWRFTIRQGVTFTNGEV
                     LDATTVKESIDLYRAPESVVASRYRSIVEVVAVDDQTVEIHTSVPDPFLPNALTDNAF
                     IMPTKYLAEVGKEQFASKPIGSGPYLLSEWVRGDRIVFSANPDYWNGVAGIQQVVWRT
                     IPEPSSRVAALQNGEVDLIWEIPAIQVPLLERTPGVQVQSGPSPRAVYIGLWPDSPVA
                     GGEPLRDVRVRQALNYAVNRQAIVQALQRGYGTLISQPIPQPTYLGYDPSIEPYPYDP
                     AKAKELLAEAGYPNGFTIELLATARYLTAEESQAVVADLAAVGVTVKLVEVEYGQFVT
                     RLTQEKSQSPMYSLSIQGTQGVDAYEIYSIAIASTGTFNWNHYTNPAVDALVTQMASE
                     FDDATRADLARQAARLTHDDPPWIFLWNNSSIYGLKDVWKWTQRSDDLISIYEDVSW"
     gene            3132..>3542
                     /locus_tag="PKN52_02405"
     CDS             3132..>3542
                     /locus_tag="PKN52_02405"
                     /inference="COORDINATES: protein motif:HMM:NF040248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ98822.1"
                     /translation="MKPRFLVSRLAQAAFVLLGVSFTVFALSHLSGDPVSLMVGPTAT
                     QADIDALRRSMNLDRPFFSQYAAFLGGVLRGDFGTSLWQHQPAIGLVLERFPYTIQLA
                     AAALLLALLVAVPLGILSAVFRNSFLDRLAIGTSL"
BASE COUNT          581 a         1279 c         1088 g          594 t
ORIGIN      
        1 gagcccaccg aacatgacgt cggcctgagg gttggcggcc tcggagcgga tgcgggtctt
       61 cagctcgccc gcgcccgcct gcacggactc caccttcacg ttcgggtact tgccctggaa
      121 gccctgctcg atgatgagct tgtactcgat gtcgtacgtc gtcgtgtaga tgaccagcgt
      181 gccggtgacg gacgggtcgc tctgggcgtt ggccaggccg gccaaggcca ccagggtcat
      241 cagggcgatc aggatgctgc gcttcatctc gttacctcct tgagtggtgc gttcttggtg
      301 tcgtctgctt cgagcacgga cagcgcggtc tcgaccgtcg cccagtccgg gatcccctta
      361 cgtccaccca tctgctcgca cttccttccc gcggcgtact gcgcgaaccg gatgcactcc
      421 tccacgcctt tcccccgcag gcgcgccgcg aggaaggccc cgtggaacac gtcgccggcg
      481 ccggtggtgt ctacgacctt cacgcggaag gcgggatagt gcctgacctc gccccccatc
      541 accgcgtaga cgccctcctg accggccgtg gcggccacga accgcggtcc ccacgtgctc
      601 atctccagga gcgcctcccc taggcttccc ttgccggtga cgaaccgcgg gtacacggcg
      661 ttggtgatga ggatgtcggc catggaggcg aggcgccggt tcagttcgtt gtcgctctgc
      721 cgcgtcgagc cgtcgagcga gacggtgacg ccgtgctcct tggcgagcga ggcggcgcga
      781 acggcgttct cgtagtgcgt cccgtcgaga tgcaggacct tcgctccgct cagcgcctct
      841 agctggcgct cgctccacgc gatgggcggc atgcggtcct tgaaggggaa cttcgtgcgg
      901 gtaccgcgct cggggctgat catcacgtag gaggagaggc tcctgacgcc ctcgaggcgg
      961 tcgagggccg agatgtcgac cccctccctc cggagctcat ccaagatctc gtcgccgatg
     1021 cggtccgcgc cgaggttacc gatcaaggca gtgtcgatcc ccaacctggc ggcggcaacg
     1081 gtggcggtgg ccgcggctcc gccgccctgg aactcgatct ccgtgatgcg cgtggagccg
     1141 tcctccggcg gatagccctc gatgacgcag aggtggtcct ggcagatatg cccaaccccg
     1201 accaactccg gcacggaaac ctccttgagg tgttctggcg gtagattaac tgcctcatca
     1261 ggaattgtca acggattgta gtaaacatat caatcgggcc atgctcgggg ctaggggccg
     1321 ttcgctgcag caagttgcgc acccaggggg cgaaaggcgt ggcccagcta accgaccgtt
     1381 cgcaaccgcg agcgcggcca gtaccgcggt ccctcgcgtg acgatcgcaa ccgtccaccg
     1441 cccggacccg aactcatgtt gtctcccaag tctcccatgt actattacac caatcactac
     1501 caggaggtga gagccgcaac agaccagcgc ttcgaggagc atagtcccag atgacctcca
     1561 gtcggccggt ataggccgcc cgtacagacg caccaaggga gcccaagatg aaagcactga
     1621 accgcctact cgccttcgcg gcggcgctgt ccctgtcggc agccctcgcg caaggcgcgc
     1681 cgcctcagtc cagcttcgtg ctcggcatgt ccgccgagac cgccactttc tacccgccag
     1741 gcaccggcgg cctgttcgag cgcaacagct tcctgcagat cttcgactcc ctcatgcacc
     1801 gcgaggcgga cggcaccgtg gtgccccgca tcgccaccgc gtgggaagcg gtcgacgcca
     1861 ccacgtggcg cttcaccatc cggcagggcg tgaccttcac gaacggcgag gtcctcgacg
     1921 cgaccacggt caaggagagc atcgacctct accgggctcc cgagagcgtc gtggcctcgc
     1981 gctaccgcag catcgtcgag gtagtggctg tcgacgacca gaccgtggag atccacacga
     2041 gcgtgcccga cccgttcctc ccgaacgcgt tgaccgacaa cgccttcatc atgccgacca
     2101 agtacttggc cgaggtcggc aaggagcagt tcgcctccaa accgatcggc tcggggccgt
     2161 acctgctcag cgaatgggtg aggggcgatc gcatcgtgtt ctccgccaac cccgactact
     2221 ggaacggcgt cgccggcatc cagcaggtag tgtggcgcac catccccgaa ccgtcctcgc
     2281 gcgtcgcggc cctccagaac ggcgaggtcg acctgatctg ggagatcccg gccatccagg
     2341 tcccgctgct cgagcgcacc ccgggcgtcc aggtccagtc cggcccgtcg ccccgcgccg
     2401 tctacatcgg cctgtggccc gacagcccag tcgccggcgg cgagccgcta cgcgacgtcc
     2461 gcgtgcgcca ggccctcaac tacgccgtga acaggcaggc gatcgtccag gcgctgcagc
     2521 gcggctacgg cacgctcatc agccagccca tcccccagcc cacctacctc ggctacgacc
     2581 cgagcatcga accgtacccc tacgatccgg cgaaggccaa ggagctgctg gcggaggccg
     2641 gctacccgaa cggcttcacc atcgagctgc tagcgaccgc gcgctacctc acggccgagg
     2701 agtcgcaggc cgtggtggcc gacctggccg ccgtcggcgt caccgtcaag ctcgtcgagg
     2761 tcgagtacgg ccagttcgtc acgcgcctga cgcaagagaa gtcccagtcg cccatgtact
     2821 ccctctcgat ccagggcact cagggcgtcg acgcctacga gatctacagc atcgcgatcg
     2881 ccagcacggg gacgttcaac tggaaccact acaccaaccc ggccgtcgac gcgctcgtca
     2941 cgcagatggc cagcgagttc gacgacgcca cgcgcgccga cctcgccagg caggccgcga
     3001 ggctcacgca cgacgacccg ccgtggatct tcctctggaa caactcctcg atctacggcc
     3061 tcaaggacgt ctggaagtgg acgcagcgct ccgacgacct gatctccatc tacgaagacg
     3121 tctcctggta gatgaagccg cggttcctgg tatcacggct ggcgcaagcg gccttcgtgc
     3181 tcctgggcgt gtcgttcacc gtgttcgcgc tgtcccacct gagcggcgac cccgtatcgc
     3241 tgatggtggg gccgacggcg actcaagcgg acatcgacgc cctgcgccgg tcgatgaacc
     3301 tggatcgtcc gttcttctcg cagtacgccg ccttcctcgg cggcgtactg cggggcgact
     3361 tcggtaccag cctctggcag caccaacccg ccatcggtct cgtcctcgag cgtttcccct
     3421 acaccatcca gctcgcggcc gccgccctgt tgctggcact cctggtagcc gtgccgctcg
     3481 ggatcctgag cgccgtgttc cgcaacagct tcctcgaccg gctggctatc ggcacctcgc
     3541 tc
//
LOCUS       DAOQYE010000089         3537 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_27564_length_3537_cov_2.744687, whole genome shotgun sequence.
ACCESSION   DAOQYE010000089 DAOQYE010000000
VERSION     DAOQYE010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 3537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3537
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27564_length_3537_cov_2.744687"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="PKN52_02410"
     CDS             <1..456
                     /locus_tag="PKN52_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98823.1"
                     /translation="IAAAMGPKPDAAPPAPPAASAVADKKDRAATDERLIAEAAAAST
                     RAYNDAAALTEGVRKDGHDGIVGAESATAKALAIIDEHLPALRVDADAALKAGNVASF
                     LRFYDAAEGKRRERLLDAQGAALVGAFRADDVGLTSAPAPFILPAAHVR"
     gene            538..1464
                     /locus_tag="PKN52_02415"
     CDS             538..1464
                     /locus_tag="PKN52_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98824.1"
                     /translation="MTQTATNRSLYRMLPGLMVTPSGAGTEPESICSPFRVQQFTFTF
                     AGADPLAAGAYTVTWVTPLNGTIVTTITSDGVIGLNAGATLLAAAINQTVGVDSLFYA
                     TASGAVVTAYAKSPNTNLAVPTTAVPGADTLTPAQSVAPSAPSLRMGLFYTYANPGTP
                     YAIIGTPRGANIAALPTGSTAIADLRGVVARAVSQTELSATFNDALTNDAYPAGSLAP
                     GLNRGEVVAVVDPASGTMTPGGEVHVVIAAGTYSVIGSVANAADGGNTIRIDNAPTGV
                     ILGRVPVDGFEETLAIGAYSVRQVKLKLNRGN"
     gene            1568..2836
                     /locus_tag="PKN52_02420"
     CDS             1568..2836
                     /locus_tag="PKN52_02420"
                     /inference="COORDINATES: protein motif:HMM:NF021467.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HNQ98825.1"
                     /translation="MSALLQFQHQIAEAGGRVYHDNANAINAVKRYHAERNDSINALL
                     AGRALNGARLDSIPAAQRMDSNGKPLTNGALAQSMATVHAFAQAFGEIIGWNDGRGRP
                     VRFDALVPVASQALTYFYSEVMEYAHSGLPAWEGKILPIDKKVASAVEEYVWYEKDLT
                     GVARAASTYAANQIPMVGGPMAQANKGKIIPFLVGMEVNFREAIQAAFARSNGKPDFQ
                     IERGKTDACQRAIAEAINFLWMYGDATLGIDGLMNHPAIPTVAINGVWSGKTALQILD
                     DFTTIINTIPNATAGQLGDRSKITIKLPPAQWQRANAIPVTAAGSETVLEFVKKTYKG
                     ITIEEEQSFASANSQIYTGGPLGLSRDRGLILYNQGDDTRDPSFVLSQVIEMPSPPRQ
                     NGLSETMFFHARAGGCKVPDARGIRFIEGL"
     gene            2912..3316
                     /locus_tag="PKN52_02425"
     CDS             2912..3316
                     /locus_tag="PKN52_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98826.1"
                     /translation="MQNLAGHPNATALCAVELQAANIRIVALDEPYGEPRSLVAGQVG
                     AFTFRRAWVYWIVDGEMSEAHARAINTAVRHPTDRDSKYSGAECTWGDVIRVDGFSGG
                     TEVTGPVDSWHIDTDDGLAFFAAKLVEFGYAT"
     gene            3353..>3537
                     /locus_tag="PKN52_02430"
     CDS             3353..>3537
                     /locus_tag="PKN52_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98827.1"
                     /translation="MAKQDELITRDPWAWQPAEQVMVLVSKRIRNVQFPRALLDKDAT
                     PEQMAKWLAPTTLASERG"
BASE COUNT          613 a         1394 c         1054 g          476 t
ORIGIN      
        1 atcgcggccg ccatgggccc gaagcccgac gctgctccgc cggccccacc cgctgcgtcc
       61 gccgtcgccg acaagaagga ccgcgccgcg accgacgagc gcctcatcgc cgaggctgcc
      121 gccgcgtcga cccgcgcgta caacgacgcc gccgcgttga ctgagggcgt tcgcaaggat
      181 ggtcacgacg gcatcgtggg cgccgagtct gcgaccgcga aggcgctcgc catcatcgac
      241 gagcacctcc cggccctacg cgtcgacgcc gacgccgcac tcaaggccgg caacgtcgcc
      301 tcgttcctgc gcttctacga cgccgccgag ggcaagcgcc gcgagcgtct gctcgatgcg
      361 caaggcgccg cgctcgtcgg ggccttccgc gcggacgatg tcggactcac ctcggcgcct
      421 gcccccttca tcctccccgc cgcgcacgtg cgctagtcgc tgcgccgctc gcacccaacc
      481 actccaactt ctcgccgctc gcacacccgc aacccatccg aaccggacac tgccgccatg
      541 acccagaccg ccaccaaccg ctcactttac cgcatgctcc ccggtctgat ggtcaccccg
      601 tcaggcgccg gcaccgagcc cgaatccatc tgctcgccgt tccgagtcca gcagttcacg
      661 ttcaccttcg ccggcgctga cccgctcgcc gcgggtgcgt acaccgtcac gtgggtcacc
      721 ccgctgaacg gcaccatcgt caccaccatc accagcgacg gcgtcatcgg cctgaacgcc
      781 ggcgccacgc tgctcgccgc ggccatcaac cagaccgtgg gcgtcgactc cctgttctac
      841 gccaccgctt cgggcgccgt cgtcaccgcg tacgccaaga gcccgaacac caacctcgcc
      901 gtcccgacca ccgcggttcc cggcgccgac accctgaccc ccgcgcaaag cgtcgccccc
      961 tcggcgccgt cgctgcgcat gggcctgttc tacacctacg ccaacccggg caccccctac
     1021 gcgatcatcg gcaccccgcg cggggccaac atcgccgcgc tgcccaccgg gtccaccgcc
     1081 atcgccgacc tccgcggcgt cgtcgctcgc gccgtcagcc agaccgaact gtccgcgacc
     1141 ttcaacgacg cactgaccaa cgacgcttac cccgccggca gcctcgcccc gggcctcaac
     1201 cgcggcgaag tcgtggccgt cgtcgacccg gcctcgggca ccatgacccc cggcggcgaa
     1261 gtgcacgtcg tcatcgcggc cggcacctac tcggtcatcg gcagcgtggc caacgccgcg
     1321 gacggtggca acaccatccg catcgacaac gcccccaccg gcgtcatcct cgggcgcgtc
     1381 ccggtcgacg gcttcgagga gaccctcgcc atcggggcct acagcgtccg ccaggtcaag
     1441 ctcaagctca accgcggcaa ctgagccacc gctcggcgcc gacgcaacag gcggtcgcat
     1501 aggtcgtcgt accctcatcc caaccgctcc atcgcgctcc aaccgtcgca caccctccga
     1561 ggtcaacatg tccgccctcc tccagttcca acaccagatc gccgaagcgg gcggccgcgt
     1621 ctaccacgac aacgccaacg ccatcaacgc cgtcaagcgc taccacgccg agcgcaacga
     1681 ctccatcaac gcactactcg ccggtcgtgc cctcaacggc gcgcgcctcg acagcatccc
     1741 cgccgcgcag cgcatggaca gcaacggcaa gccgctgacc aacggcgcgc tcgcgcagtc
     1801 gatggccacc gtacacgcct tcgcccaagc cttcggcgag atcatcgggt ggaacgacgg
     1861 ccgcggtcgc ccggtccgct tcgacgccct cgtgcccgtc gcaagccaag cgctgaccta
     1921 cttctactcc gaggtcatgg agtacgcgca cagcgggctc ccggcgtggg aaggcaagat
     1981 cctccccatc gataagaagg tcgccagcgc cgtcgaggaa tacgtctggt acgagaagga
     2041 cctcaccggc gtcgcgcgtg cggctagcac ctacgcggcc aaccagatcc ccatggtcgg
     2101 cggcccgatg gcgcaggcga acaagggcaa gatcatcccg ttcctcgtcg gcatggaagt
     2161 caacttccgc gaggccatcc aggccgcgtt cgcccggagc aacggcaagc ccgacttcca
     2221 gatcgagcgc ggcaagaccg acgcatgcca gcgggcgatc gccgaggcca tcaacttcct
     2281 gtggatgtac ggcgacgcga cgctcggcat cgacggcctg atgaaccacc cggccatccc
     2341 gaccgtcgcc atcaacggcg tgtggtcggg caagacggcg ctgcagatcc tcgacgactt
     2401 cacgaccatc atcaacacca tcccgaacgc gaccgcgggc cagctcggcg accgcagcaa
     2461 gatcaccatc aagctgcccc cggcgcagtg gcagcgggcg aacgccatcc ccgtcaccgc
     2521 ggccggcagc gagaccgtgc tggagttcgt gaagaagacc tacaagggca tcaccatcga
     2581 ggaggagcag tcgttcgcca gcgccaactc gcagatctac acgggcggcc cgctcggcct
     2641 gagccgtgat cgcggcctca tcctgtacaa ccagggcgac gacacgcgcg acccgtcgtt
     2701 cgtgctgtcg caggtgatcg agatgccgtc cccgccgcgc cagaacggcc tgagcgagac
     2761 gatgttcttc cacgcgcgcg ccggcgggtg caaggtcccg gacgcgcggg gcatccggtt
     2821 tatcgagggc ctgtagtcct cggcggcact gcccgcagcg aagccgcccg ccctagtggc
     2881 gcggtggctt ttgtcatgct aggatgctgg catgcaaaac ctcgccggac accccaatgc
     2941 gacggccctc tgcgccgtcg aactgcaggc cgccaacatc cgcatcgtcg ccctcgacga
     3001 gccctatggg gaaccccgct cactcgtcgc tgggcaggtc ggcgcgttca ccttccgccg
     3061 tgcgtgggtc tattggatcg tcgacggcga gatgagcgag gcccacgccc gcgcgatcaa
     3121 caccgcggtg cggcatccaa ccgaccgcga cagcaagtac agcggcgcag agtgcacgtg
     3181 gggcgacgtg attcgcgtcg acggcttctc gggtgggacc gaagtgaccg gcccagtcga
     3241 ctcgtggcac atcgacacgg atgatggtct cgcgttcttc gccgccaaac tcgtcgagtt
     3301 cggatacgcg acgtagcggc aacccgcccg cgccgcgtcc actctcgcgg cgatggcgaa
     3361 gcaagacgaa ctcatcacgc gcgacccgtg ggcgtggcag ccggccgagc aggtcatggt
     3421 cctcgtgtcc aagcgcatcc gcaacgtgca gtttccgcgg gcgctgctgg acaaggacgc
     3481 gacccccgag cagatggcga agtggctcgc gccgacaacg ctggccagcg agcgcgg
//
LOCUS       DAOQYE010000090         3529 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_27654_length_3529_cov_2.831606, whole genome shotgun sequence.
ACCESSION   DAOQYE010000090 DAOQYE010000000
VERSION     DAOQYE010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 3529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3529
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27654_length_3529_cov_2.831606"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..886)
                     /locus_tag="PKN52_02435"
     CDS             complement(<1..886)
                     /locus_tag="PKN52_02435"
                     /inference="COORDINATES: protein motif:HMM:NF014114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HNQ98828.1"
                     /translation="MRGPLVQPPKLRPGDKVAVLSPSFAAPAVAPEVHEQALRRFAEL
                     TQLVPVEFPTTRKLGAPAVERAADFNAALADREIRAILASIGGEDQIRVVPHLDAGLL
                     RGDPKPFLGYSDNTNILNWFWTNGVAGFYGGSTQVHLGPGPHVDPEHLVSLTAALLTG
                     GVLEVSEPGESEDFGRDWNDPRALVEFGERDATEPWTWAGPTRTVSGSTWGGCLEVID
                     WIALAGRMPANHDLSGAILLLETSEDLPPERMVRWWVRALGERGVLAAVAGVLVARPP
                     SSALGAPLPSAGERAAWRA"
     gene            complement(923..2593)
                     /locus_tag="PKN52_02440"
     CDS             complement(923..2593)
                     /locus_tag="PKN52_02440"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HNQ98829.1"
                     /translation="MTELLVVLVTGLLVIAGATVIGPRFGVASPLVLVAVGVAASFLP
                     LFGAFEIDPEWVLEGLLPPLLYSSAVSLPTMNFRRDFGTISGLSVLLVVGTALVLGLF
                     FMLVVPGLGFTWGVALGAIVSPTDAVATSIVKRSNVSKRVATILDGESLLNDATALVL
                     LRTAIVATAASFSFWGSVGTFAYSVVLALLIGAAVGVLNLAVRRRLTDATVNTVISFA
                     VPFVASIPAELLGASGLVAAVVAGLMTGLRAPRVLSPRNRLSDSQNWHTVELVLEGAV
                     FLIMGLQLTSILTHVHTDHAGIGAAVRVAGGALLLTILVRAAYVAPLLGFLAARGRLY
                     GKIRPRFQGLQERISTPEGRQLTLDRMNAAGRKATEEDLDRFAVRVTRGLADIQYLLR
                     APLGWREGTVVVWAGMRGAVTVAAAQTLPADTPQRSVLVLIAFAVAVGSLLLQGGTLG
                     LLLRVVAPGAGQAALAEEAAAERARIVDLLRETGDGVPEPTIPAGATRQEQYALATDH
                     RLTVLAAQRSALLDARDNGVFDADVLEDALIDLDAAQIAIELRGRYAH"
     gene            2846..3325
                     /locus_tag="PKN52_02445"
     CDS             2846..3325
                     /locus_tag="PKN52_02445"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNQ98830.1"
                     /translation="MRPEVSLRTLTTDDLSAVLALELDEGQDKFVAPNEYTLAEAYLA
                     LTTSEFTPRVYGIFLGDEAVGLIAIAHQPAETEGDAPFYEMYRFMVDRRHQRRGIGRR
                     ALQQAIDILRTKPLGPGSHVATSFVPGNDIAKALYLQLGFRETGELDGDEIVAVMPL"
BASE COUNT          586 a         1243 c         1192 g          508 t
ORIGIN      
        1 ccgcgcgcca ggcggcgcgc tcgccggcgc tcggcagcgg cgcaccgagc gcgctggaag
       61 gcggccgcgc caccaacacg cccgcgaccg cggcgaggac gccacgctct cccagtgcgc
      121 ggacccacca gcgcaccatg cgctcaggcg gcaggtcctc gctagtctcc agcagcagga
      181 tggctcccga aaggtcgtgg ttcgccggca ttcggcccgc caaggcgatc cagtcgatca
      241 cttccaggca cccgccccac gtgctgccgc tgaccgtgcg agtcggacca gcccacgtcc
      301 acggttcggt ggcgtcgcgc tccccgaact cgaccagcgc gcgagggtcg ttccagtcgc
      361 gtccgaagtc ctcggactca cccggttcgc tcacttccag gacgccaccg gtcaacagtg
      421 ctgccgtcaa cgagaccaag tgctcggggt cgacgtgcgg gcccggcccc aggtgcacct
      481 gcgtcgagcc gccgtagaag ccggctacgc cattggtcca gaaccagttc aggatgttcg
      541 tgttgtcgct gtacccgagg aacggcttcg ggtcgccccg caggagcccg gcgtccaggt
      601 ggggcacgac gcggatctgg tcctcgccac cgatgctcgc caggatggcc cgtatctcgc
      661 ggtccgccaa cgccgcgttg aagtcggcgg cgcgctccac cgcaggcgca cccagcttgc
      721 gcgtggtcgg gaactcgacc ggcaccagct gggtgagctc cgcgaagcgt cgcaacgcct
      781 gctcgtgcac ctcaggcgcg acggccggtg cggcgaacga cggcgagagg accgccacct
      841 tgtcgcccgg gcggagcttg ggtggctgga cgagcgggcc gcgcatcggc ctagagctta
      901 ctcgaccgcc cccagtgacc cctcagtgcg cgtaccggcc cctcaactcg atagctatct
      961 gggcggcgtc gaggtcgatc agcgcgtcct cgaggacgtc ggcgtcgaag accccgttgt
     1021 cgcgcgcgtc gagcagcgct gatcgctgag cggcgaggac ggtcaagcga tggtcggtgg
     1081 cgagcgcgta ctgctcctgc cgggtggcgc ccgcggggat ggtgggttcc gggacgccgt
     1141 ccccggtctc tcgcagcagg tccacgatcc tggcacgctc ggcggcggcc tcctcggcga
     1201 gcgccgcctg acccgcgccg ggcgcgacga cccgcagcag cagtccaagg gtaccgccct
     1261 gcagcaggag cgaccccacg gcgaccgcga aggcgatcag gaccagcact gaccgttgtg
     1321 gggtgtccgc ggggagcgtc tgggccgcgg ccacggtgac cgcaccgcgc atgccggccc
     1381 acacgaccac ggtcccttcg cgccagccga gcggcgcgcg caggaggtac tggatgtcgg
     1441 caagcccgcg ggtgacgcgt acggcgaacc gatccaggtc ctcctcggtg gctttgcgcc
     1501 cggcggcgtt catccggtcg agcgtcagct gcctgccttc cggcgtgctg atgcgctctt
     1561 ggaggccctg gaaacgcggt cggatcttcc cgtaaagccg gccgcgcgct gccaggaaac
     1621 cgagcagggg cgcgacgtaa gctgccctca cgaggatcgt caggagcaag gcacccccag
     1681 ccacccgcac ggcggcgcct atgccggcgt ggtcggtatg aacgtgcgta aggatcgagg
     1741 tgagctgcaa ccccatgatg aggaacacgg cgccctcgag cacgagttcc accgtgtgcc
     1801 agttctgcga gtcggacagc cggttgcgtg gcgacagcac cctcggcgcg cgtagaccgg
     1861 tcatcaggcc ggcgaccacg gccgcgacga ggccggacgc gcccagcagc tcggccggga
     1921 tggaggccac gaagggcacg gcgaaggaga tgaccgtgtt cacggtcgcg tcggtcaggc
     1981 gccgccggac agccaggttc aacacaccta cggccgcccc gatcaggagc gcgagcacga
     2041 cggagtaggc gaaggtgcct acggagcccc agaaggagaa cgaggccgcc gttgcgacga
     2101 tggcggtacg cagcagcacg agcgcggtcg cgtcgttgag aaggctctcg ccgtccagta
     2161 tcgtcgccac gcgcttggac acgttggagc gcttcacgat ggaggtcgcc accgcgtcgg
     2221 tcggactgac gatcgcgccg agcgccacgc cccacgtgaa cccgaggccg ggcacgacga
     2281 gcatgaagaa caggccgagc acgagcgcgg tgccgacgac gaggagcacg gacaggccgc
     2341 tgatcgtccc gaagtcgcgg cggaagttca tcgtcggcag cgacacggcc gaggagtaca
     2401 gcaacggcgg cagtagtccc tccaggaccc actccggatc gatctcgaag gctccgaaca
     2461 gcggcaggaa gctcgcggct accccgacgg cgaccagtac gagcggcgag gcgacgccga
     2521 agcgcgggcc gatgaccgtg gcgccggcga tcaccaacag ccccgtaacc agtacgacta
     2581 gcagttctgt cacgcggtcg tcccctccat acgtgtcctc ccggtcgcgt cctcggcatt
     2641 atcgccggcg atccgaccta ttccgtacgc acgagtcgct ctcaccacgc atccgcgact
     2701 cggttcggtg gagtcggttc agaaacccag tcgtggtgcc acaggcgacg cccctgcacc
     2761 ggcctgggtc gtgacgattc gttcatcttt cgtaccggcg tcggggttgc ttcacggcag
     2821 tccgccagtg cactatgcgt gcagcatgcg accagaagtc tcgctacgaa cgttgacgac
     2881 cgacgacctc tcggcggtcc tggcactgga gttggacgaa ggccaggaca agttcgtggc
     2941 cccgaacgag tacacgctgg ccgaggcgta cctggccctc acgaccagcg agttcacccc
     3001 tcgcgtctac gggatcttcc ttggcgacga ggcggtaggc ctgatcgcga tagcccacca
     3061 gccggccgag acggaaggtg acgcgccgtt ctacgagatg taccggttca tggtcgaccg
     3121 tcgccaccaa cgccgcggca tcggcaggcg cgccctgcaa caggccatcg atatcttgcg
     3181 caccaagccc ctcggcccgg gaagccacgt cgccaccagc ttcgtgccgg gcaacgacat
     3241 cgccaaggcg ctgtacctgc aactcgggtt cagggagacc ggtgagttgg acggcgacga
     3301 gatcgtggcc gtgatgccgc tatgactggc ggccctttga cggcagacgg atgaacggta
     3361 aacccagagc cgccaggtcc atgaacggag cctctcccat gctcgccgca gccttgatcg
     3421 tgcgcttcat ccttgagctt gccctgctgg cgggcgtcgc cctcctctcc ttctacctcg
     3481 tgcaaggctg gctgcgcttg ccggtgacga tcctcgctcc tatcgcagt
//
LOCUS       DAOQYE010000091         3520 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_27737_length_3520_cov_2.802886, whole genome shotgun sequence.
ACCESSION   DAOQYE010000091 DAOQYE010000000
VERSION     DAOQYE010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 3520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3520
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27737_length_3520_cov_2.802886"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            107..1900
                     /gene="uvrC"
                     /locus_tag="PKN52_02450"
     CDS             107..1900
                     /gene="uvrC"
                     /locus_tag="PKN52_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HNQ98831.1"
                     /translation="MKRGDLPVIPTQSGCYLFHAEDGTVLYVGKAVNLRSRVGSYFGR
                     QAGQKARLLTRAASHLDFIVTQDEVEALILESNLIKRHQPHYNVLLKDDKSYPFLKLT
                     NEEFPTLIFTRRVVKDGGTYYGPYPNAGAVRRVLELVYSVFKLRKNSGVPMETRKKPC
                     LRYHMGRCHAPCVGLADKAEYADVVSDVKAFLEGRVVEIVEKLEVQMREAAGRRDFEL
                     AATYRDRLDALKRLTGYDSSVVMAGVDDLDFLGVARAGDHAMVQLFQMRSGRVVGRDK
                     RLMTNAESASDAEVLERFMSEYYGLATQLPPLVLVPQTDLDQGVWQRFLSARAGHKVE
                     VRTPQRGDKVELLELAKRNAVSGVEAEIALLERRGEAPGVGELQRLLGLENPPWRIEG
                     YDISNLMGTHTVASIVSFEGGRAKKNDYRRVRVRELDKPDDFFSMHQVVYRRFTGAMA
                     DKLPPPDLLLIDGGKGQMSAAKRALDEAGVKVPIVGLAKKQETLITERGEEVLVPHSH
                     PALRLLINVRDEAHRTAVGYNRQRRGKSATRSLLDDVPGIGPKRRDALLAHFSSLEEL
                     KGAEVGVLAAIPGVGQAAAQAVKSYFEGSDD"
     gene            complement(1876..2457)
                     /locus_tag="PKN52_02455"
     CDS             complement(1876..2457)
                     /locus_tag="PKN52_02455"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNQ98832.1"
                     /translation="MRPAYGRVTLRPLEEFSDADWRRLQSHFRDPEIAHLNGTPPNRM
                     PLWLLKRLLKTDSKRPDRATFGIFDERDDFIGTTELYDVRGDIGTLGIIIGERTHWAG
                     GYGPEAMHALLGYAFDVLGLGTVRLNTFGDNLRAQRAFRKVGFREVDRRPDADGRIDV
                     RMELARAEWRPGPDHGLGAGQLGRTAQSSDPSK"
     gene            complement(2653..>3520)
                     /locus_tag="PKN52_02460"
     CDS             complement(2653..>3520)
                     /locus_tag="PKN52_02460"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNQ98833.1"
                     /translation="ADVTKRLLDTYNVDFAILTGNAGLLGVAAHPNSRFGVSFAQAYN
                     DWLIEDWLNVEHRFKGSMMVSQLDMAAAVAEVERIGHHKGIVQVILPSNSGRLYGDRF
                     YWPLYEVAAAHGLPIAIHPSGYTVLPPTSAGWPTTYLEAHTMIATEYLNHMISLASQG
                     PFEEFPELKFVFIEGGVATFSPMLWRLEKNWKAVRAEVPWLTTSPREYLRTNFRFTTQ
                     PIEEPEDPELLRRIFADLRAERSVMFASDWPHWDFDDPEAALRPLPIALRNRILGENA
                     VDLYGLRDAAPS"
BASE COUNT          603 a         1157 c         1209 g          551 t
ORIGIN      
        1 acgagacctg ggcggctgcc ggaaggcggc tgcccgaagg cggtaggccg gtcgacgggc
       61 ccgcggcgaa ccggcagttg aggcccccag ccgtacaatc tccggcgtga agcgcggcga
      121 cctgccagtg atccccacgc agagcggctg ctacctgttc cacgccgagg acggcacggt
      181 cctctacgtg ggcaaggcgg tcaacctgcg cagccgcgtc ggcagctact tcggccgcca
      241 ggcggggcag aaggcgcggc tgctcaccag ggcggcgagt caccttgact tcatagtcac
      301 gcaggacgag gtcgaggcgc tcatcctcga gtcgaacctc atcaagcgcc accagccgca
      361 ctacaacgtc ctattgaagg acgacaagag ctaccccttc ctcaagctga cgaacgagga
      421 gttcccgacg ctgatcttca cgcgccgcgt ggtgaaggac ggcggcacct actacgggcc
      481 gtacccgaac gccggcgccg tcaggcgcgt gctcgagctc gtctacagcg tcttcaagct
      541 gcgcaagaac agcggcgttc ccatggagac gcgcaagaag ccgtgtctgc gctaccacat
      601 gggccgctgc cacgccccct gcgtcggcct ggccgacaag gctgagtacg ccgatgtcgt
      661 gtcggacgtg aaggccttcc tggagggccg cgtggtggag atcgtcgaga agctcgaggt
      721 ccagatgcgc gaggcggcgg ggcggcgcga cttcgagctg gcggccacct accgcgaccg
      781 cctcgacgcc ctcaagcgcc tcacgggcta cgacagcagc gtcgtcatgg ccggcgtgga
      841 tgacctcgac ttcctcggcg tcgcgcgtgc cggcgaccac gccatggtgc agctcttcca
      901 gatgcgctcc gggcgcgtgg tcgggcgcga caagcgcctc atgaccaacg cggagagcgc
      961 gtcggacgcc gaggtgctcg agcggttcat gagcgagtac tacgggctcg ccacgcagct
     1021 ccctccgctc gtgctcgtac cccagacgga cctcgaccag ggcgtctggc agcgcttcct
     1081 ctccgctaga gccggtcaca aggtagaggt gcgcacgccc cagcggggcg acaaggtgga
     1141 actcctggag ctggccaagc gcaacgccgt ctcgggcgtc gaggccgaga tcgcgctgtt
     1201 ggagcggcgc ggcgaggcgc cgggcgtcgg cgagctgcaa cgcctgctcg ggctcgagaa
     1261 cccgccgtgg cgcatcgagg gctacgacat ctccaacctg atgggcacgc atacggtcgc
     1321 gagcatcgtg tcgttcgagg gcggcagggc gaagaagaac gactaccgcc gcgtgcgcgt
     1381 gcgcgaactc gacaagcccg acgacttctt cagcatgcac caggtcgtct accgccgctt
     1441 caccggggcc atggccgaca agctgccccc gcccgacctg ctcctgatag acggtggcaa
     1501 gggccagatg agcgcggcca agcgggccct cgacgaggcg ggggtcaagg tgcccatcgt
     1561 cgggctcgcc aagaagcagg agacgctcat cacggaacgt ggcgaggaag tgctcgtgcc
     1621 gcacagccat ccggcgctgc gcctgctcat aaacgtccgc gacgaggcgc accgcacggc
     1681 cgtcggctac aaccggcagc ggcgcggcaa gtcggccacg cgctcgctcc tggacgacgt
     1741 gccgggcatc gggcccaaac ggcgcgacgc actgctcgcc cacttctcga gcctggagga
     1801 gctgaagggt gctgaagtcg gcgtgctcgc cgccatcccg ggcgtagggc aggcagccgc
     1861 ccaggcggtg aagagctact tcgaggggtc agacgactga gctgtgcggc cgagctgtcc
     1921 ggcgccgaga ccgtggtcgg gaccgggccg ccactccgcg cgcgccaact ccatccggac
     1981 gtcgatgcgg ccgtccgcgt ccggtcggcg gtcgacttcc cggaagccca ctttgcggaa
     2041 ggcgcgttgg gcgcgcaggt tgtcgccgaa cgtgttgagc cgcacggtgc ccaagcccag
     2101 tacgtcgaac gcgtagccca acagcgcatg catcgcctcg ggcccgtaac cgccggccca
     2161 gtgcgtgcgc tccccgatga tgatgccgag cgtgccgatg tcgccgcgca catcgtagag
     2221 ctcggtcgtg ccgatgaagt cgtcgcgctc gtcgaagatg ccgaaggtgg cccggtccgg
     2281 ccgcttggag tccgtcttga gcagccgctt gagcagccat agcggcatgc ggttcggcgg
     2341 agtgccgttc aagtgggcga tctccgggtc gcgaaagtgg ctctgcaggc gccgccagtc
     2401 ggcgtcggag aactcctcca gcggccgcag cgtcacgcgc ccgtaggccg gtctcatacg
     2461 ggcctaagtg tacatagccc ccagtgcgcg tgagacgaag cttgcctcaa tcccgacaaa
     2521 cgggcacggc ctgctctcaa gttctgcggt cgcgatcagc ggccgttcca tgccgtgacc
     2581 ggatcggctg cgagtagcag tccgcgaatc ccagcatgct gttcatgccg catgtcggcg
     2641 ctgtcgctcc ggtcaggatg gcgctgcgtc gcgcagaccg tacaggtcca cggcgttctc
     2701 tccaagaatc ctgttcctga gggctatggg cagcgggcgg agcgcagcct cgggatcgtc
     2761 gaagtcccaa tgaggccagt cagaggcgaa catcaccgat cgttccgcac gcaggtctgc
     2821 gaagatacgg cggagcagct ccgggtcctc cggctcttcg atcggttgcg tagtgaacct
     2881 gaagttcgtc cggagatact ctcggggcga ggtggtcagc cacggcacct cggccctgac
     2941 ggccttccag ttcttctcga gtcgccacag catgggcgag aaagtagcga cgccgccctc
     3001 gatgaacacg aacttgagct cggggaactc ctcgaaggga ccctggctgg ccaagctgat
     3061 catgtgattg aggtactcgg tggcgatcat ggtgtgggct tcgagatagg tggttggcca
     3121 gccggccgag gtcggcggga ggaccgtgta gccggagggg tggatggcta tcggcaggcc
     3181 atgagccgcc gcgacttcgt agagtggcca gtagaacctg tcgccgtaga gccgcccgga
     3241 gttggagggc agaatgacct ggacgatgcc cttgtggtgc ccgattcgct cgacttctgc
     3301 tacggcggct gccatgtcca actgggagac catcatcgac cccttgaagc ggtgctcgac
     3361 gttcagccag tcttcaatga gccagtcgtt gtatgcctgc gcgaacgaca cgccgaagcg
     3421 agagttcggg tgggcggcta ctcctagcag gcccgcgttg ccggtgagga tggcgaagtc
     3481 cacgttgtag gtgtccagca atcgcttggt cacgtctgca
//
LOCUS       DAOQYE010000092         3514 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_27814_length_3514_cov_2.770454, whole genome shotgun sequence.
ACCESSION   DAOQYE010000092 DAOQYE010000000
VERSION     DAOQYE010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 3514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3514
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27814_length_3514_cov_2.770454"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /locus_tag="PKN52_02465"
     CDS             <1..702
                     /locus_tag="PKN52_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98834.1"
                     /translation="LAALRAAYELLADPDTSLGVVALENPDGYALHRELCAVHAEHMH
                     HAARYSALGDDLEYRGDEAPFESAVRRRLLALGGAQLLINLHGYPAHEWVRPLTGYLP
                     HKFEWWAVPQGFILIARYQPGFAERAESLLRTVARAVQTALPELVAFNDVQLAAYEAH
                     AGKLLFPLEGSVPLVLTETARPGPAVQLITEYPDETVHGAAFRLAHEVQYRVAVGAAR
                     AWPAALAGLPPLDSR"
     gene            898..1746
                     /locus_tag="PKN52_02470"
     CDS             898..1746
                     /locus_tag="PKN52_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98835.1"
                     /translation="MRALVLVGLFSGFAYAQLGWSDIPAEGPRLEAFVYPPQVRFGEA
                     ALVLHCQLGTFFPVLKLGLGLVETRADYVWVETFHDGELTGAGAWRFDPDGARAMPLD
                     ARHSMAVADALLNGGSLRVAASLTDGEPALSYSFGLDDYQNLRSQLPCLSNTEHTLQV
                     DEWRYYPDVGLVFGGDRRDKGVVFYCTNQLTVGLYFHADSDPLMPTLNVWIGGATPDY
                     FAFQRVGEGFYEGDGWAMLRTFEAMANSRSVSFGMVSAAWPYVSVSNYAGLVAALGNL
                     SCLDAQ"
     gene            1868..2536
                     /locus_tag="PKN52_02475"
     CDS             1868..2536
                     /locus_tag="PKN52_02475"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNQ98836.1"
                     /translation="MEQDPLAVHRDSIYALPEFVAAANGLLADYLPKDAAGRAADEVT
                     PRLVRFYTTEGLMPEAHKEGREARYSYEHLLSLLVVRKLLAQGFGGAAIKRAVAGTGA
                     DELEALLKEDVQLQLVPRVTSGPLDPAKAAFLQKVRSKAGLRVQAPAPAAVPAAPVEP
                     SAPARPPEVATTWTRVTVQDGLEVLARDDFAMPDTPQGDQELLQALKVALLQVGQATK
                     KRKS"
     gene            2635..>3514
                     /locus_tag="PKN52_02480"
     CDS             2635..>3514
                     /locus_tag="PKN52_02480"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNQ98837.1"
                     /translation="MTASQRPYPRPRVQLVPAKPGLPVEGGKLDVLVTIGVDHPAVSA
                     QRQPLSLALVIDRSGSMTGPPLEEAKRAAQLAVSMLLPGDWVSVVAFDDAVRVPQALV
                     RVGDDRRALQNAITAIGPGSSTNLFAGWAEGLSQVLTCPEEGVVSRVVLLSDGMANVG
                     VTDSAQISSDVAEASVHGVTTTTMGLGRSYDEALLRAMADAGRGNYVFLADERAMGLA
                     FEEEIAGLSALRGRNVRLVARPSDATLLRHADPVMAAAVGLAPDAHGVALPDLVAGMP
                     IDQLLTLEVTPGQHDLK"
BASE COUNT          541 a         1186 c         1196 g          591 t
ORIGIN      
        1 ctcgccgcgc tgcgcgccgc gtacgagctc ctcgccgatc cggacaccag cctcggggtc
       61 gttgccctgg agaaccccga cgggtacgcg ttgcaccgtg agctctgcgc cgtccatgcc
      121 gagcacatgc accatgcggc ccgttactcg gcgctcggtg acgacctcga gtaccggggc
      181 gacgaggcgc ctttcgagag cgcggtccgg cggcgcctct tggcgcttgg cggcgcccaa
      241 ctcctgatca acctgcatgg ctaccctgcg cacgaatggg tgcgcccgct cacgggttac
      301 ctgccgcaca agttcgagtg gtgggccgtg ccgcagggct tcatcctcat cgcgcgctac
      361 caaccgggtt tcgccgagcg ggctgagtcg ctcctgcgca cggtggcgcg cgcggtgcag
      421 accgcccttc ccgagctcgt ggctttcaac gacgtgcagc tggccgcgta cgaggcgcac
      481 gccggtaagc tcctcttccc gctggagggg agcgttccgc tcgtcctgac ggagacggcg
      541 cgtcctgggc cggcggtgca gttgatcacc gagtacccgg acgagaccgt ccacggcgcc
      601 gccttccgcc tagcgcacga agtgcagtac cgggtagcgg tcggtgctgc acgcgcgtgg
      661 ccagcggcgc tggctgggct accgcctctc gactcccgtt gaacctgact cgacgagccg
      721 cttactggcc tcgagcttcc gggtctgctg cccccagatc aggaacgacc gtcctgcgcg
      781 ggtatgggac gcttcctgtc cgatttgtcg ctgctgccag taacgagaac gcctagcatt
      841 tgagcaacga ccgttcgaaa cgcctggtct tcacaagggg ggatcatgtc gcatctggtg
      901 cgtgcgctcg tcttggtggg tctgttctcg ggctttgcct acgcccagct cggatggagc
      961 gatatcccgg ccgaagggcc gcgcttggag gccttcgtat atccgcccca agtgcgtttc
     1021 ggagaggccg cactggtgct gcattgtcag ctcggcactt tcttcccggt cctgaagctg
     1081 gggctcggcc tggtcgagac gcgggccgat tacgtttggg tagagacgtt ccacgacggt
     1141 gagctcacgg gtgccggcgc ttggcggttc gacccggacg gtgctcgagc catgcctctg
     1201 gatgcccggc attccatggc agtagccgac gcgctgttga acggcggctc gcttcgggtc
     1261 gcggcgagtc tgactgatgg tgaaccggcg ctcagctaca gcttcggtct cgacgactac
     1321 cagaacctgc ggtcccaact accctgcctg agtaacacgg agcacacgct gcaggtcgac
     1381 gagtggcgct actaccctga tgtaggactc gtgttcggcg gcgaccgccg cgacaagggc
     1441 gtggtctttt actgcacgaa ccagttgacc gtaggcctgt acttccacgc cgactccgac
     1501 ccgttgatgc ctaccctcaa cgtatggatc ggtggtgcga ccccggacta tttcgccttc
     1561 cagcgtgtgg gagagggatt ctacgagggc gacggctggg ccatgttgag gaccttcgag
     1621 gccatggcga actcgcgctc cgtcagcttc gggatggtga gtgcggcgtg gccttacgtg
     1681 tccgtatcga actacgcggg gctggtggcc gccctcggca acctgtcttg cttggacgct
     1741 caatgatgag cggctagaac gagctgcgca ccgcctgccg ggaccgcggg ccacggcgtc
     1801 ccgactcgga cgtggtgaca gcagcaactg accttgacag ctatagctgt tagtgtaagc
     1861 tatgaccatg gagcaagacc cgctggccgt ccaccgcgat tcgatctatg cactcccgga
     1921 gttcgtcgcc gcggccaacg gcttgctggc cgactaccta ccgaaggacg cggccgggcg
     1981 tgccgcggac gaggtgaccc cgcggctcgt ccgtttctac acgacggagg gcctgatgcc
     2041 ggaagctcat aaggagggtc gggaagcccg ctactcctac gagcacctcc tctctctcct
     2101 ggtcgtgcgc aagctcctcg cgcaagggtt cggcggcgcg gcgatcaagc gcgccgtggc
     2161 gggcacgggg gcggacgaac tggaggcgct cctgaaggag gacgtccagc tgcagctcgt
     2221 tccgagggtc acgagcgggc cgctcgaccc cgcgaaggcg gcgttccttc agaaggtacg
     2281 gtcgaaggcg ggcctgcggg tccaggcgcc ggcgccagcg gcggttcccg cggcgccggt
     2341 cgagccgagc gccccggcgc gaccacccga ggtcgccacc acgtggaccc gcgtcaccgt
     2401 ccaggacggg ctcgaagtac tcgcgcgcga tgacttcgcc atgccggaca cccctcaagg
     2461 cgatcaggag ctgttgcagg ccctcaaggt ggcgttgctc caggtcggtc aggcgaccaa
     2521 gaagcgcaag agctgatcgg cagcggcctc ctggggcatg tagtgccccg gggagtcaca
     2581 actactcccg tgcggcggcg agggccgaca cgcacgcctc ccaaggggga tccgatgaca
     2641 gcaagccagc gaccgtaccc gcgaccccga gttcaactcg tgccggccaa gccgggtctc
     2701 ccggtcgaag gcgggaagct cgacgtcctc gtgacgatcg gcgtcgatca tcccgctgtc
     2761 agcgcgcagc gtcagccgct cagcttggcg ctcgtgatcg accggtccgg ctccatgacc
     2821 ggtccgccgc ttgaagaagc caagcgcgcc gcgcagctcg ccgtctccat gctgctgccg
     2881 ggcgactggg tgtcggtcgt cgcgttcgac gacgccgtgc gagtacccca ggccctcgtg
     2941 cgagtagggg acgaccggcg cgcgctccag aacgccatca ccgcgatagg tcccggcagt
     3001 agcacgaacc tcttcgctgg ctgggcggag ggcctctctc aggttctcac ctgcccggag
     3061 gagggcgtag tcagccgcgt ggtgctcctg agcgacggca tggcgaacgt cggggtgacc
     3121 gacagcgccc agatctcgag cgatgtggcc gaagcgtccg tccacggggt gaccaccacg
     3181 acgatgggcc tcggccgcag ttacgacgag gcgttgctgc gcgccatggc cgacgccggc
     3241 cgcggcaact acgtcttcct ggcggacgag agggcgatgg ggctggcgtt cgaggaggag
     3301 atcgccggac tgagcgcgct gcgtggtcgc aacgtgaggc tcgtggccag gccgagcgac
     3361 gccacgctgc tacgacacgc cgatccggtc atggccgccg ccgtcgggct cgcgccggac
     3421 gcgcacggcg tggcgctccc cgacctggtg gccggcatgc cgatcgacca gctccttacc
     3481 ctcgaagtga cacccgggca gcacgacctg aaac
//
LOCUS       DAOQYE010000093         3512 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_27832_length_3512_cov_3.349436, whole genome shotgun sequence.
ACCESSION   DAOQYE010000093 DAOQYE010000000
VERSION     DAOQYE010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 3512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3512
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27832_length_3512_cov_3.349436"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1110)
                     /locus_tag="PKN52_02485"
     CDS             complement(<1..1110)
                     /locus_tag="PKN52_02485"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNQ98838.1"
                     /translation="MIRKLSALFAVSALLLAACTPPALTAHGFSPAGAATDVPVDTTV
                     SATLNIAPLAASVVNALTLTSGEEATPVAGSVAYNSSTRTITFTPAAPLEYATTYTAS
                     LSANIRTSDGTRISGSRTWSFTTEDAPATGVVSVTIDQGDISLLTGETATLTTTVEVD
                     GEVPETVTWSSDDETVASVSNDGVVTAVASGTATVTATSTADTSVSDSVIVTVADEPA
                     VISVAIVEGDQELYLGADLQLSADVQAVGGADASVTWESDDETVATVSDTGLVSSVGL
                     GTAVITATSVADPALADSVTVTVVVAPAVTDLSIDGTDRVVTLSDDVAFTATVTVEGS
                     ATDAVTWTSSDETVATIDPDTGEATLVGAGDTIIAA"
     gene            complement(1294..2400)
                     /locus_tag="PKN52_02490"
     CDS             complement(1294..2400)
                     /locus_tag="PKN52_02490"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNQ98839.1"
                     /translation="MTEQRGGHVAVFLPNLAGGGAERVAVDLVNNFAARGEAVDLITA
                     QATGPLLKDVGPGVTLTDLGRKRVAAAFFPLLAHLLRTRPSALLATLEHANVMAGWVA
                     TFAPKLRLVLREANTLSRDLSANSLGNRLLLGAMRNAYRRADAIVAVSAGVATDLTTV
                     IGVPEEKIHVVGNPVLTTRVTQGAAVKPEHRFFAPGQPPVVLGVGRLAPQKRFGVLLE
                     AVAAVRESRPCRVLILGDGEEREALERRIVELGLQQDADLPGFSANPFSAMAAAAVFV
                     LSSAWEGLPNVLIQAMSLGTPAVATDCQSGPGEITDQGRLAKLVPVDDVAALAAAIAG
                     SLDAPRRPPPPEWLERYDSDLVVEKYLQILRGGA"
     gene            complement(2397..>3512)
                     /locus_tag="PKN52_02495"
     CDS             complement(2397..>3512)
                     /locus_tag="PKN52_02495"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNQ98840.1"
                     /translation="ITFLLPALTMGGAEAQLLSVLERDPSALARYSVRVLIITSQRHP
                     IIDARLAALGIPVDVIDRESLSFPGFLVTLVHHFRSSAPSAVHVFLAGSTSTWGRLAA
                     LLAGVQVVIYSDLSLNPTTTRAQRLLTPLLHRLTKRFLPNARAIANRLEREGASRERI
                     VLLRNGVELGRFTPEGPDLRSTLGITPRARVVGFLGMLRPAKRPDLFFSSVLTLPEGQ
                     RPDLLLMAGDGALRSDVERQIAEDDWLRDHASLLGVVEDTPAFLRTLDLLVLTSDTEG
                     LPNAVLEAMSVGVPCVATAVSDVPELLEGVGLVVPAGDARAIGDAIAKALSMSESSWT
                     PRLEAGRSRIEHEYDMNIAAERFWRAHDELLLAGDAR"
BASE COUNT          584 a         1126 c         1228 g          574 t
ORIGIN      
        1 cgccgcgatg atcgtgtcgc ctgcgccgac caacgtggcc tcgccggtat ccggatcgat
       61 ggtggcgacg gtttcgtcgg agctagtcca cgtgaccgcg tcggtggcgc tgccttccac
      121 cgtgaccgtg gccgtgaagg cgacatcgtc ggagagggtc accaccctgt ccgtgccgtc
      181 gatgctcagg tcggtgacag caggggccac caccacggtg accgtgacgg agtcggccag
      241 tgccgggtcg gccacgctgg tggccgttat gaccgcggtg ccaagaccga cgctgctgac
      301 gaggccggta tcggacacgg tcgcgacggt ctcgtcgtcg gattcccagg tcacggacgc
      361 gtcggcgcct ccgacggcct gaacgtcggc cgacagttgc aggtcagccc cgaggtacaa
      421 ctcctgatcc ccctcgacga tggcgacgct gatcaccgcc ggttcgtccg cgacggtcac
      481 gatcacggag tcggacaccg aggtgtcggc cgtgctcgtg gcggtcacgg tggccgtccc
      541 actggccacg gcggtgacca cgccgtcgtt cgatacggag gcgacggtct cgtcgtcgga
      601 gctccaagtg accgtctcag ggacctcgcc atcgacctcg acggtcgtgg tcaaggtagc
      661 ggtctcaccc gtcagcaggc tgatgtcgcc ctgatcgatc gtcacgctga cgacgccggt
      721 cgcaggggcg tcctccgtcg tgaagctcca ggtacgcgag cccgagatgc gcgtgccatc
      781 ggacgtcctg atgttcgcgg acagggacgc cgtgtaagtg gtcgcgtact cgaggggagc
      841 cgcgggggtg aaagtgatcg tacgcgtgga actgttgtaa gcgacgctgc cggcgaccgg
      901 ggtcgcttcc tcgccggaag tgagggtcag ggcgttgacc acggaggcag ccagcggcgc
      961 gatgttgagc gtggccgaca ccgtcgtgtc gaccggcacg tcagtcgctg ccccggcggg
     1021 cgagaaccca tgggctgtca gcgccggggg agtgcatgcg gccaagagga gtgcggagac
     1081 ggcgaacaac gcagacagtt tcctgatcat ctcaatccct tctaagaaac caaagatcga
     1141 acgcgcgaag tcgttctctt ctgtcgatac gacacacgcc tcctcacgtt gggcaggcgc
     1201 gaatcctcgt ccagcctaca cgaggcaaca tgaggattca gccagtatgt cggctaaaac
     1261 gccttgtgcg ttttctcacg tcggacggag gattcatgct ccgccgcgca ggatctgcag
     1321 gtacttttcc accacgaggt cgctatcgta gcgctccaac cactcggggg gcgggggtcg
     1381 cctgggtgcg tcgagggagc cggcgatagc ggcggctagg gccgccacgt cgtccacggg
     1441 gactaacttc gcgagcctgc cctggtccgt gatctccccc gggccgctct ggcagtcggt
     1501 cgcgacggcc ggcgtgccaa gcgacatcgc ctggatcagc acgttcggga gcccttccca
     1561 cgcagaggag agcacgaaca cggcagctgc cgccatggcc gagaaggggt tggcggagaa
     1621 accaggcagg tccgcgtcct gctgcagccc caactcgacg atgcgtcgct cgagcgcttc
     1681 ccgctcttcg ccgtcgccca ggatgaggac acggcacggt cgactttcgc gcaccgcggc
     1741 taccgcttcc agtagaacgc cgaagcgctt ctggggagcg aggcggccta ctccgagcac
     1801 gacgggtggt tggccggggg cgaagaagcg gtgctcaggt ttgacggcgg ctccctgcgt
     1861 aaccctcgtc gtcaagaccg ggttacccac cacgtgtatc ttctcttccg ggacaccgat
     1921 taccgtcgtg aggtcggtgg cgacgccggc ggagacggcg acgatggcgt ccgcgcggcg
     1981 ataggcgttg cgcatagcgc ccagcagcag acggttgccc aggctgttcg cggacaggtc
     2041 ccgcgacagg gtgttggcct ccctgagcac gaggcgaagc ttcggagcga acgtcgcgac
     2101 ccagcccgcc atgacgttgg cgtgctcgag cgtcgccaag agcgcgctcg gccgggttcg
     2161 cagcaggtgc gccaagagag ggaagaacgc cgcggccacg cgcttgcgcc ccaggtcggt
     2221 gagcgtgacg cccggtccca cgtccttgag gagcgggccg gtggcctgtg ccgtgatcag
     2281 gtcgacggct tcccccctgg cggcgaagtt gttgacgaga tcgacggcga cgcgctcggc
     2341 tccgccgccg gcgaggttcg ggaggaatac ggcgacgtgt ccaccgcgct gctccgtcat
     2401 ctggcatctc cggcgagcaa caactcgtca tgcgcccgcc agaaacgctc ggccgcgatg
     2461 ttcatgtcgt actcgtgttc gatgcgcgag cggccggcct cgagtctggg cgtccaggag
     2521 ctctcgctca tggagagtgc cttggcgatg gcgtcaccga tggcccgagc gtcgccggcc
     2581 ggaaccacca gtccgacccc ttcaagcagc tctggtacgt ccgatacggc cgtggcgacg
     2641 cagggcacgc cgaccgacat cgcttcgagg acggcgttcg gcagaccttc cgtatccgaa
     2701 gtcaggacca gtaggtcgag ggtcctgagg aaggccgggg tgtcctcgac cacccctaac
     2761 aggctggcat ggtcccttag ccagtcgtcc tccgcgatct ggcgttcgac atcgcttcgt
     2821 agggccccgt cgcccgccat caggagcagg tctgggcgct ggccctccgg gagtgtgagc
     2881 acggaggaga agaacaggtc ggggcgcttg gccggccgca gcatgccgag aaagcccact
     2941 acgcgcgctc gcggggttat gccgagtgtc gaccgcaggt cgggaccttc cggtgtgaag
     3001 cgtcccagtt cgacgccgtt gcgcagtagg acgatccgct cgcgtgaggc tccttcccgc
     3061 tcgaggcggt tcgctatggc tcgagcgttg ggtaggaacc gcttcgtcag gcgatgtaac
     3121 agtggcgtca gtaaacgttg ggcccgggtc gtggtcgggt tgagggacag gtcggagtag
     3181 atgaccacct gcacaccggc caacagcgcg gccaggcgcc cccacgtgga tgtggagccg
     3241 gccaggaaca cgtggaccgc cgacggggcg gaggacctga agtggtgcac gagggtgacc
     3301 aggaagcccg ggaagctcag cgactcccgg tctatcacat cgacgggtat gcctagcgca
     3361 gccagccgcg cgtcgattat cggatggcgt tgagacgtga tgatgagcac ccgcaccgag
     3421 tacctagcca gcgcgctcgg gtcgcgctcc agaacggaga ggagttgagc ctccgcgccg
     3481 cccatggtga gcgccgggag taggaacgtg at
//
LOCUS       DAOQYE010000094         3507 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_27895_length_3507_cov_2.613557, whole genome shotgun sequence.
ACCESSION   DAOQYE010000094 DAOQYE010000000
VERSION     DAOQYE010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 3507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3507
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27895_length_3507_cov_2.613557"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /locus_tag="PKN52_02500"
     CDS             complement(<1..661)
                     /locus_tag="PKN52_02500"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNQ98841.1"
                     /translation="MSGALTDASARRTALTDTDHGLLVEAGAGSGKSAILAGRVALLL
                     AAGVAPRRIAAITFTELAAAELATRVRDYVTELAGGSVPPPLEPAFATTAEPPSPTRE
                     QRANLLAALESLDELTSTTIHGFAGELTRPYPVEAGADPGATVMDAPAADALFSDVFD
                     AWLRRRLGTSDGTPDPLVRLLMRPAAPGPDTLRKLAGLLRSHPDTRSRPVDLAVVARE
                     FA"
     gene            complement(658..2892)
                     /locus_tag="PKN52_02505"
     CDS             complement(658..2892)
                     /locus_tag="PKN52_02505"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HNQ98842.1"
                     /translation="MALASTLTGRVVLERLTDLAPLYRGLMAALAAHVPVTWRGASGE
                     RPTWLPASIDWVAPSTARPRLRGASCADPDHEALEAIRWAHELLANGRRAADIAIAAV
                     DPTAYDEALAVYARGAGIPLHSAHGVPVASTPLGQVLAALADLLARGVSQTRVRRLLA
                     TARAAQTGPLALVPETWADELDAEAFLQRPVQWERALRPLAAREPEQAAIIMRLVVDL
                     ALGFAAAERIGDRWLDGRARSLWRLALTAGPAAVLERSLATMRVVDETDPAIAVVWGP
                     ASALVAWPRPFTRLVGLSGRAWPRRGNDEDPLLPERLRHGARLRERNTAREDSEAFVA
                     LTTGASSELVASWPRRGSDGRRLTRSPLIDRLPGLATEVAVRSVTRHASCEGDRRAAR
                     PRELATDLDHRMALTAFRSRFSASLTSLDGLVRERHPAVVKALEGKHSATSLRSLLDN
                     PHAFIAKYALGWRRPEPQTEVLALDAAALGTLLHEVLRVATEETAGERSWPSDTQGVQ
                     AAVRRAVKAVSDEWEATRPVPPWALWRAVMNEITEWASWSLALDADTRGGGETYAELE
                     FGHDSGRGGRQTSTPWDPASEVLVPGTGLRVRGVIDHLDVDRTAKRVRVIDYKSGKPP
                     REPAGLDGGTELQRTLYTVVVRQLLGADWEVEALLVHPRQRQCFRLEDPDACAATLTE
                     AIRLAVESLIAGNVVAGPGLGSPFAETAIAFPAYGTARYLETKGEALSSLRRELEDVL
                     RGVA"
     gene            complement(2909..3316)
                     /locus_tag="PKN52_02510"
     CDS             complement(2909..3316)
                     /locus_tag="PKN52_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98843.1"
                     /translation="MATAFERVTYVVPNRLAERRVRDELAAGGSSGHAVTGIAGLASR
                     LAGGLRRSASATEVLAALRDPPASVLTSLRDVALLPGFGQAAARTLFAAWQADLDLQA
                     RADVGGRRRLHGRAPAPCRGLATARRPATAAVG"
BASE COUNT          489 a         1247 c         1300 g          471 t
ORIGIN      
        1 cggcgaactc ccgcgccacc accgccaggt cgaccggacg cgagcgcgtg tccggatgcg
       61 agcgcaacag ccccgccagt ttccgcagcg tgtccggtcc gggcgccgcg gggcgcatca
      121 gcagacgtac cagggggtcc ggcgtgccgt cgctcgtccc caagcgcctg cggagccagg
      181 catcgaagac atcggagaag agcgcgtcgg ccgcgggagc atccatcacg gtagcgccgg
      241 ggtccgcacc ggcctccacc gggtaagggc gggtcagctc gccggcgaag ccatggatgg
      301 tcgtgctcgt gagctcgtcc agactctcga gggcggctag gaggttggcg cgttgctcac
      361 gggtcgggga gggaggctcg gcggtggtgg cgaacgccgg ctcgagcggt ggtggaacgc
      421 tcccgccggc cagctcggtc acgtagtcgc gcacccgcgt ggccagctcc gccgcggcca
      481 gctcggtgaa cgtgatggcg gcgatgcggc gcggcgccac cccggcggcg agaaggaggg
      541 cgacccgccc tgcgaggatg gcagacttcc cggagccggc accggcctcc acgagcagcc
      601 catggtcggt gtcggtgagc gccgtgcgcc tggcggaggc gtcggtgagg gcgccgctca
      661 cgccacgcct cgcagaacgt cctcgagttc ccggcgcaga gacgagaggg cttcgccctt
      721 ggtctcgagg taacgggcgg tcccgtaggc cgggaacgcg atcgccgtct cggcgaacgg
      781 cgaaccgagt cccggcccgg cgacgacgtt gcccgcgatg aggctctcga ccgccagcct
      841 gatcgcctcg gtcagggtgg ccgcgcaggc gtcgggatcc tcgagcctga agcactgtcg
      901 ttgtctgggg tgcacgagca gcgcctcgac ctcccagtcg gcgcctagga gttggcgcac
      961 gacgacggtg taaagggtgc gctgcagctc cgtgccgccg tccagtccgg ccggctcgcg
     1021 cggcggcttg ccggacttgt agtcgatcac ccgcacgcgc ttggcggtgc ggtcgacgtc
     1081 gaggtggtcg atgacgcccc tgacacgcag gccggtgcct gggacgagca cctccgaagc
     1141 tggatcccac ggcgtagacg tctgccgacc gccacgcccc gagtcgtgac cgaactccag
     1201 ctcggcgtac gtctcgccgc caccacgggt gtctgcgtct aaggcgagcg accagctcgc
     1261 ccattcggtg atctcgttca tgacagcgcg ccaaagcgcc catggcggaa ccggacgtgt
     1321 ggcctcccac tcatcgctca ccgctttcac cgcgcgcctt actgccgcct gcaccccttg
     1381 ggtgtcacta ggccagctcc gctcacctgc cgtctcttcc gtagcgaccc gcaacacctc
     1441 gtgcaggagg gtgccgagcg cggccgcgtc caacgcgagg acttccgtct gcggctcggg
     1501 ccttcgccag ccgagggcgt actttgcgat gaaggcgtgc gggttgtcga gcaacgaccg
     1561 gagcgaggtg gcggagtgct tgccttcaag cgccttgacg acggccggat gccgctcgcg
     1621 gacgagcccg tcgaggctcg tgagagacgc cgagaagcgc gaccggaacg ctgtcagcgc
     1681 catccggtgg tcgaggtcgg tggccagctc gcgcggtcgg gccgcccgcc ggtcgccctc
     1741 gcaggaggcg tggcgggtga cggacctgac cgcgacctcg gtcgcgaggc ccggcagccg
     1801 gtcgatgagc gggctcctgg tcaacctccg gccgtcggat cctcggcggg gccaggaggc
     1861 gacgagctcc gagctcgctc cggttgtgag cgcgacgaaa gcctcggagt cctcccgcgc
     1921 cgtgttgcgc tcccggagcc gggcgccgtg gcggaggcgc tcagggagga gggggtcctc
     1981 gtcgttgccg cgacgcggcc aggcccggcc gctcagtccg acgagcctgg tgaacgggcg
     2041 cggccaagcg accagcgccg acgcaggacc ccacacgacg gcgatcgcgg ggtccgtctc
     2101 gtcgaccacg cgcattgttg ccagcgatcg ttccaggacc gcggccggtc ccgccgtcag
     2161 cgccaggcgc cagagcgatc gcgcccgacc gtccagccac ctgtctccga tcctctccgc
     2221 cgcggcgaag cccaaggcga ggtcgacgac gaggcgcatg atgatggcgg cctgttccgg
     2281 ctcgcgcgcg gccaaggggc gcagggcgcg ctcccactgc acggggcgtt ggaggaacgc
     2341 ctccgcatcg agttcgtccg cccatgtctc tggcactagg gccagcgggc ccgtctgggc
     2401 cgcccttgcg gtcgccagca ggcgcctgac gcgggtctgc gagacgccgc gtgcgagcag
     2461 gtccgcgagc gcggccagta cctgcccgag cggcgtgctc gcgaccggaa cgccatgcgc
     2521 cgagtgcagg gggatgccgg cgccacgggc atagactgcc agcgcttcgt cgtaggcggt
     2581 cgggtcgacg gccgcgatgg cgatgtcggc cgcgcggcgg ccgttggcga ggagttcgtg
     2641 tgcccaccgg atcgcctcga gcgcctcgtg atcggggtcg gcgcagctcg cgccgcgcag
     2701 gcgcggccgt gcggtcgagg gggccaccca gtcgatgctc gccgggagcc aggtcggccg
     2761 ctcgccgctg gcgccgcgcc aggtgaccgg cacatgggcg gccagtgccg ccatgagccc
     2821 acgatagagg ggcgccaggt cggtcagtct ctccaagacg acgcgtcccg tgagggtgct
     2881 ggcgagcgcc acgcgggcac gtgcggcctc aaccaaccgc ggcggtagca ggacgccgtg
     2941 cggtagcgag gcctcgacat ggcgcaggtg ctcgaccatg aaggcggcgg cgcccgccaa
     3001 cgtctgcgcg cgcctgcagg tcgaggtcgg cctgccaggc ggcgaacaac gtgcgcgcgg
     3061 cggcctgacc gaagccgggt aggagagcca cgtcgcgcag gctcgtaagg accgaggccg
     3121 gcggatcgcg cagagcggcg aggacctcgg tcgcgctggc cgagcgccgg agacctccgg
     3181 cgagccggct cgccaggccc gcgatgccgg tgacggcgtg gccgctgctc ccaccggcag
     3241 ccagctcgtc gcggacgcgg cgctcggcca gccggttcgg gacgacgtag gtgacgcgct
     3301 cgaaggccgt tgccatctca ggtacgggga ggatagcccg ccggcgcgac aggtagcgcc
     3361 cacggtgctg cattacggtc gccctccgac gccttgcgat accattcgcc gcgtgagcag
     3421 cgccccgatc tccatccgcg accggaacta catcctcgcc ggcatcctcc tggccatgtt
     3481 cctcgcggcc atcgaccaga cgatcgt
//
LOCUS       DAOQYE010000095         3487 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_28128_length_3487_cov_3.296911, whole genome shotgun sequence.
ACCESSION   DAOQYE010000095 DAOQYE010000000
VERSION     DAOQYE010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 3487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3487
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28128_length_3487_cov_3.296911"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            325..654
                     /locus_tag="PKN52_02515"
     CDS             325..654
                     /locus_tag="PKN52_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98844.1"
                     /translation="MDQEPSGTRARRYLVTTDHGDVVVTVNPAAGGLDHDLLTLEPAT
                     PEARAGIAMATPLRAFGAKMLDLIEIQGLGDAGLSPGLRDMLMREKATQDLKRIERFA
                     KSAVAGG"
     gene            complement(681..1589)
                     /locus_tag="PKN52_02520"
     CDS             complement(681..1589)
                     /locus_tag="PKN52_02520"
                     /inference="COORDINATES: protein motif:HMM:NF012265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative dsRNA-binding protein"
                     /protein_id="HNQ98845.1"
                     /translation="MDKDGVNMAHPKGLLIERAQRLGLPRPEFDTAMTGPEHEPSFLS
                     DVTFDGELLGTGQGGSKREAERRAAEEALAALDKREAAASSDAKPKTGAKPKADAKGA
                     AKGAAKVTKAAKKPADAKASDDAQSADGDATRKGPQSATGAQAAKVAKGGAKSAAGAA
                     AGGSKTAPKEARRQTGGKQAAAAPSATASGATASGGTASKGAAAPQAPAARAARPAGE
                     PAAVQAQPATPASKREDAEPFTGPWPMIDDVLASVITVAERRVSADLRGEAALFAIRD
                     FALRLYKDVLADLGEVVEEEEEDEEE"
     gene            1824..2489
                     /locus_tag="PKN52_02525"
     CDS             1824..2489
                     /locus_tag="PKN52_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNQ98846.1"
                     /translation="MTRLLLSDDHAMFRQGLRSILETEEDFRVIGEASTGREAVRYAL
                     ETRPDVILMDIQMPELDGVAATKAILAEWPDARVIILTMYRQDRYVFEAIKVGARGYM
                     LKDAGATELVTAIRRVAAGETLLNAEMAASILDEFRKLGDLPSHPEHKLSELTEREED
                     ILRLLAQGATNQEIATSLNVSEKTVRNRLSEIFSKLRLNNRTQAALYALREGIATLQD
                     SQQ"
     gene            2486..>3487
                     /locus_tag="PKN52_02530"
     CDS             2486..>3487
                     /locus_tag="PKN52_02530"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNQ98847.1"
                     /translation="MTKRPGAQGASARGKADAVEDVRVLGALLLDIAQTPAPTGSEGE
                     RAALVLRLWRAAGLEARLDAVGNVVAEVPASGAATRPRSAGVARTVVLAAHLDSVFGP
                     EVDVSVKRGEERWSGPGIGDNAASLAVLTRYLQTRDQPGRSVRPRLVVAATVGEEGLG
                     DLRGAKHLVAGLGREADAFIAFDGVLGSITDAAVGSTRYEARFTAPGGHSWGDYGAAS
                     AVHAAGEAIAALRRVPVPSVPRSSLNVGQVWGGTSVNAIAERAGFNLDLRSLDAAALM
                     GLEEAALAAVRAAAKATGAQVELIRVGARQAGRSANAWLVAAARAAYDDVGVTSRASA
                     "
BASE COUNT          491 a         1248 c         1229 g          519 t
ORIGIN      
        1 atccagtgcc cgaacgccgg gatgatgctg gatgccagcg acgggccgag gatgatgccg
       61 gcaagcagtt cgccaacgac ggacggttgg ttgaagcgtc gcgccaactc gccgagcgcg
      121 cgcgcggcga acagcagtag ggcgacctgc acgagcaggt gcaggacgtc tccgtgaggg
      181 gctgcggtga acggtgccgt ggtcgtctcc ttctcgttcc gcgaggtcgc ggcctatggc
      241 gggctagacg atacccgacg agcgctgcgc ttggcatgta accaggacag ccgctcgggg
      301 tcggctacgt gctagcgtcg cccggtggat caggaaccgt ccggaacgcg cgccaggcgc
      361 tacctcgtga ccacggatca cggagatgtc gtcgtgaccg tcaacccggc ggcaggcggg
      421 ctcgaccacg acctgctcac gctggaaccg gcgacgcccg aggcgagggc gggcatcgcg
      481 atggcgacgc cgctgcgcgc gttcggcgcc aagatgctcg acctgatcga gatccagggc
      541 ctgggtgacg ccggcttgag ccccggcctt cgcgacatgc tcatgcgcga gaaggccacg
      601 caggacctca agcggatcga gcgtttcgcc aaatcggccg tcgccggggg ttgagcgctt
      661 ccgacggtgg gatcgcagcc ttactcttcc tcgtcctctt cctcttcctc gacgacctcg
      721 cctaggtccg cgagcacgtc cttgtagagc cgcagtgcga agtcgcggat cgcgaagagc
      781 gccgcctcgc ctcttaggtc agcgctgacg cgcctctctg ccacggttat cacgctggcg
      841 aggacgtcgt cgatcatggg ccatgggccg gtgaacggct cagcgtcctc gcgcttggaa
      901 gcgggagtcg cgggctgggc ctgtacggcc gccggctcgc cggcgggacg cgccgcacgg
      961 gccgcaggcg cctgtggcgc tgccgcgccc ttggaggccg tgccgcccga cgccgtagcg
     1021 cccgacgccg tagcgctcgg cgccgcggcg gcctgcttgc ctccggtctg acgccgggct
     1081 tccttcgggg ccgttttgga gccacccgcg gcagcgcccg ccgcgctctt ggcgccgccc
     1141 ttcgccacct tggcggcttg cgcgcctgtg gcgctctgcg ggcctttgcg tgtcgcatcg
     1201 ccgtccgcgc tctgcgcatc gtcggaggcc ttcgcgtcgg ccggcttctt ggcggctttg
     1261 gtgactttcg cagcgccctt cgcggcgccc ttggcgtcgg ccttcggctt ggcgcccgtc
     1321 ttgggcttgg cgtctgagct cgccgccgcc tcccgcttgt cgagcgcggc caacgcctcc
     1381 tcggcggctc gccgctcggc ctcacgcttg gagccgccct gcccggttcc aagcaactcg
     1441 ccgtcaaacg tcacgtcgct caggaacgac ggttcgtgct cagggcccgt catcgccgtg
     1501 tcgaactccg gcctcggcag cccgagccgc tgggcgcgct cgatcagcaa ccccttgggg
     1561 tgcgccatgt tcactccatc cttgtccata ctgtctcgta tagggagcgc ggtcgatgtc
     1621 gaccgtctcc attcgcgcaa ctgccggacg cccgccgtct ccgacgaccg cctccgtgcc
     1681 atgtgcggcg gtgaggatgc gagtggcggt gacgccgcgt gcccggcagc tgcccgggaa
     1741 gggtagcacg cccgggcgcc ccacggcggc aggcccgcca acgcgagcgg gccgagcgcg
     1801 gctcgtgcga tacgattggg tccatgaccc gcctgctgct aagtgacgac catgccatgt
     1861 tcaggcaagg cctccggagc atcctcgaga cggaggagga cttccgcgtc ataggcgagg
     1921 cctccaccgg tcgcgaggcc gtccgctacg ccctcgagac acgcccggac gtcatcctca
     1981 tggatatcca gatgccggaa ctcgacggcg tcgcggccac caaggccatc ctcgccgaat
     2041 ggccggacgc ccgcgtgatc atcctcacca tgtatcgcca ggacaggtac gtgttcgagg
     2101 ccatcaaggt cggcgctcgc ggctacatgc tgaaggacgc cggcgccacg gaactcgtga
     2161 cggccatcag gcgcgtcgcc gccggcgaga cgctcctcaa cgccgagatg gccgcctcga
     2221 tcctcgacga gttccgcaag ctgggcgatc tcccgagcca tccggagcac aagctcagcg
     2281 agctcacgga gcgcgaggag gacatcctca gactcctggc gcagggcgcc acgaaccagg
     2341 agatcgcgac ctccctgaac gtcagcgaga agacggtccg caaccggctc tcggagatct
     2401 tctcgaagct gcgcctgaac aaccgcacgc aggcggcgct ctacgccttg cgcgaaggca
     2461 tcgccacgct gcaagactcg caacagtgac caagagaccc ggcgcgcaag gcgcgtccgc
     2521 acggggcaag gcggacgccg tagaggacgt gcgggtcctg ggcgccctgc tcctcgacat
     2581 cgcccagacg ccggcgccga ccggctccga gggcgagcgc gcggccctcg tactgcgact
     2641 ctggcgcgcc gccggtctcg aagcgcgcct tgacgccgtc ggcaacgttg tggccgaggt
     2701 cccggcctcc ggggccgcta cccgaccgcg gtccgccggg gtggccagga ccgtggtcct
     2761 cgcggcccac ctggacagcg tgttcggccc agaggtggat gtgagcgtca agcggggcga
     2821 ggagcgctgg agcggtcctg gcataggcga caacgccgcc tctctcgccg tactgaccag
     2881 gtacctccag acgcgcgacc agccagggcg ctcggtccgg ccgcgcctgg tcgtcgccgc
     2941 cacggtcggc gaggagggcc ttggagacct gcgcggtgcc aagcacctcg tggccgggct
     3001 gggtcgcgag gccgatgcct tcatcgcctt cgacggtgtc ctcggctcca tcacggacgc
     3061 cgcggtcggc tcgacccgct acgaggcgcg cttcacggcg ccgggtggcc actcgtgggg
     3121 cgactacggc gcggccagcg ccgtgcacgc cgcaggggag gccatcgccg ccctgcggcg
     3181 cgtgccggtg ccaagcgtcc cgcggtcgag cctaaacgta gggcaggtct ggggcggcac
     3241 gagcgtcaac gccatcgcgg agcgggccgg gttcaacctc gacctcagga gcctggacgc
     3301 agccgcgctg atgggcctcg aggaggccgc gctggccgca gtacgcgcgg ccgccaaggc
     3361 gacgggagcg caggtcgagc tgatacgggt gggcgcgcgg caggcggggc ggtcggccaa
     3421 cgcctggctg gtggccgccg cgcgcgccgc atacgacgat gtcggcgtga cgtcgagggc
     3481 ctcggcc
//
LOCUS       DAOQYE010000096         3473 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_28293_length_3473_cov_2.822996, whole genome shotgun sequence.
ACCESSION   DAOQYE010000096 DAOQYE010000000
VERSION     DAOQYE010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 3473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3473
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28293_length_3473_cov_2.822996"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1328
                     /locus_tag="PKN52_02535"
     CDS             <1..1328
                     /locus_tag="PKN52_02535"
                     /inference="COORDINATES: protein motif:HMM:NF013109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HNQ98848.1"
                     /translation="WPVISGFTTGIAVIIFLQQLPNVLGVPQPHGESIVLIAFRTVRE
                     YLAAPGAAPVALALLTMVVMVGWGRVKRLGFLPASMAALIIVSLVSLLAPFADVARIG
                     EIPRSLPLPALPQLNIGGAWVELSRAALAIAVLSALESLLCAVVADGMTVGEKHDPDR
                     ELVGQGLGNIAAGLFGGIPSTAALARTAVNVRAGARSRLAAIVHGLTLLVVILFLAPL
                     ASRIPLAVLGGILMVVAVRMVERESSSLILRSTKSDAFVLILTMVVTILFDLILAIEV
                     GLVSAAILFIVRMSNMFTVDPITLSGGEPWHDTDEDIEAARSLLTKHVVAYRVDGPVF
                     FGAAERFIDQLVKVDDSIKVVVLRLKRVPVMDATGVTALRSIHDRLARRGIALLVSGL
                     QPQPKALLRRMGVYEEITRDGEADFVTTDLALEVAAKKLADAGGQASAN"
     gene            complement(1443..>3473)
                     /locus_tag="PKN52_02540"
     CDS             complement(1443..>3473)
                     /locus_tag="PKN52_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNQ98849.1"
                     /translation="TKNNPVLIGEPGVGKTAIAEGLALRIVNKDVPEGLQDKRIVQLD
                     MGSLLAGAKYRGEFEERLKAVIEETARSEGKIILFIDELHTIVGAGKAEGAVDAGNML
                     KPPLARGELRMIGATTLEEYRTIEKDAALERRFQPIVVDEPSVEETISILRGIKDKYE
                     LHHGVDITDPAIVAAATMAHRYISDRRLPDSAIDLIDEAASRIQVLLDSLPEEIDDLR
                     RRKLQLEIEHEALRRESDVDSAARLLRIEEELKVVTDQIGQAQADWEAERALLEEFVA
                     DKEQLDQVKTQIESAEREYDLETAAKLRYGELPKLEAEVRRLTERLESAKYARLNVGE
                     EEVAQVVARATRIPVARLLEGEREKLLQLEEELHQRVVGQDEAITAVADAIRRSRAGL
                     ADPNRPIGSFIFLGPTGVGKTETAKALAAQLFDSEEKMIRLDMSEYMERHNVAKLIGA
                     PPGYVGYEEGGQLTEAVRRQPYSVILLDEIEKAHPDVFNTLLQVLDDGRLTDAQGRTV
                     DFRNTVVIMTSNIGSPQILEASRRGADYQQLKETVFNELRAHFRPEFLNRVDEVIVFH
                     ALDQRQIRTIAALQVERLQRRLDERRVRLELSEPALDELARIGFDPVFGARPLKRAVR
                     DYIETPLAREILAGRIVDGDVVEVLPGSGPASAGSEPGPVLRFQVVAAPAAN"
BASE COUNT          456 a         1208 c         1204 g          605 t
ORIGIN      
        1 cgtggcccgt catcagcggc ttcaccacgg gcatcgccgt catcatcttc ctgcagcagc
       61 tccccaatgt gctcggcgtg ccccaaccgc acggcgagag catcgtgctg atcgcattcc
      121 gcaccgtcag ggagtacctg gccgcgccag gcgcggcccc ggtcgcgctc gcgctcctga
      181 cgatggtcgt catggtcggc tggggccggg tcaagcggct cggcttcctc ccggccagca
      241 tggcggcgct gatcatcgtg tcgctcgtct cactgctcgc gccgttcgcc gacgtggccc
      301 gcatcggcga gataccgcgc tccctcccct tgccggccct gccacaactc aacatcggcg
      361 gcgcctgggt ggaactcagc cgcgcggcgc ttgccatcgc ggtgctctcg gcgctcgaga
      421 gcctgctctg cgccgtcgtg gccgacggca tgacggtcgg ggagaagcac gaccccgacc
      481 gcgagctggt cggtcagggc ctcggcaaca tcgcggccgg tctcttcggg ggcatcccgt
      541 ccaccgccgc cctcgcccgc accgccgtca acgtccgcgc cggggccagg tcgcggttgg
      601 cggcgatagt ccacgggctc acgctgctcg tcgtcatcct gttcctcgcg ccgctcgcgt
      661 cgcgcatccc gctcgccgtg ctcggcggga tcctcatggt cgtggccgtg cgcatggtcg
      721 agcgcgagtc gtcctcgctg atcttgcgca gcacgaagtc ggacgccttc gtgctcatcc
      781 tcacgatggt cgtgaccatc ctcttcgacc tcatcctagc gatcgaggtc gggctcgtct
      841 ccgcggccat cctcttcatc gtgcgcatga gcaacatgtt caccgtcgac cccatcacgc
      901 tctccggcgg cgagccgtgg cacgacacgg acgaggacat cgaggcggcg cggagcctgt
      961 tgaccaagca tgtcgtcgcc taccgcgtcg acgggcccgt gttcttcggg gccgcggagc
     1021 gcttcataga ccagctcgtc aaggtcgacg acagcatcaa ggttgtcgtc ctccgcctga
     1081 agcgcgtgcc cgtcatggac gcgacgggag tgacggcact acgctccatc cacgaccgct
     1141 tggcgcggcg cgggatcgcg ctgctcgtct ccggtctgca gccgcagccc aaggcgttgc
     1201 tcagacgcat gggggtgtac gaggagatca cgcgcgacgg cgaggccgac ttcgtcacta
     1261 ccgacctcgc gctcgaggtg gcggccaaga agttggccga cgccggagga caggcgtcgg
     1321 ccaactgaag gagtggtccc gggccacctc cggccgggcg cggtcggttc gaccgggcgc
     1381 ggtcaggttc gaccgggcgc cgtcggttcc gacccggcgc ggtcatccct gaccggcagc
     1441 ggtcagttcg ccgccggcgc cgcgactacc tggaaccgca gcacgggacc gggctcggaa
     1501 ccggccgagg ccggccctga gccaggcagc acttccacga cgtcgccgtc gacgatgcgc
     1561 cccgcgagga tctcacgcgc caatggggtc tcgatgtagt cgcggacggc gcgcttgagg
     1621 ggccgggcgc cgaagaccgg gtcgaagccg atgcgcgcca gctcgtcgag cgcgggctcg
     1681 gacagctcga gccgcacgcg gcgctcgtcg aggcggcgtt gcagacgctc gacctggagg
     1741 gcggcgatgg tgcggatctg acgctggtcg agcgcgtgga acacgattac ctcgtcgacg
     1801 cggttgagga actcgggccg gaagtgcgcg cgcagctcgt tgaagacggt ctccttgagc
     1861 tgctggtagt cggcgccgcg gcggctcgcc tcgaggatct gcggcgagcc gatgttgctc
     1921 gtcatgatga cgacggtgtt gcggaagtcg accgtcctgc cctgcgcgtc ggtcaggcgg
     1981 ccgtcgtcga ggacctgcag cagcgtgttg aagacgtccg ggtgggcctt ctcgatctcg
     2041 tcgaggagga tgacggagta cggctggcgc ctgacggcct ccgtcagctg gccgccctcc
     2101 tcgtagccga cgtagcccgg aggggccccg atgagcttgg cgacgttatg gcgctccatg
     2161 tactcggaca tgtcgagccg gatcatcttc tcctcgctgt cgaagagctg ggcggcgaga
     2221 gccttggcgg tctccgtctt gccgacgccg gtcgggccta ggaagatgaa ggacccgatg
     2281 gggcggttgg ggtccgccag gccggcgcgc gagcgccgga tggcgtcagc gacggccgtg
     2341 atggcctcgt cctgccctac gacccgctgg tggagctcct cctcgagctg caggagcttc
     2401 tcgcgctcac cttcgagcaa ccgcgccacg gggatgcgcg tggcgcgcgc caccacctgc
     2461 gccacctcct cctccccgac gttcaggcga gcgtacttgg cgctctcgag ccgctccgtc
     2521 aggcgcctga cctcggcctc gagcttgggc agctcgccgt agcggagctt ggcggccgtc
     2581 tccaggtcgt actcgcgctc ggccgactcg atctgcgtct tgacctggtc gagctgctcc
     2641 ttgtcggcga cgaactcctc caggagcgcg cgctcggcct cccagtcggc ctgcgcctgg
     2701 ccgatctggt cggtcacgac cttcagttcc tcctcgatgc gcaggaggcg cgcggcggag
     2761 tcgacgtccg actcacgcct cagggcctcg tgctcgatct cgagctggag cttccggcgc
     2821 ctgaggtcgt cgatctcctc gggcagcgag tcgaggagca cctggatgcg gctggcggcc
     2881 tcgtcgatga ggtcgatggc cgagtccggc aggcggcggt cggagatgta gcggtgcgcc
     2941 atcgtggcgg cggcgacgat ggccgggtcg gtgatgtcca ccccgtggtg gagctcgtac
     3001 ttgtccttga tgccgcgcag gatggagatg gtctcctcca cgctcggctc gtccaccacg
     3061 atgggttgga agcgccgctc cagggccgcg tccttctcga tggtgcggta ctcctccagc
     3121 gtggtggccc cgatcatgcg caactcgccg cgcgcgaggg gcggcttgag catgttgccg
     3181 gcgtcgacgg cgccctcggc cttgccggcg ccgacgatgg tgtgcagctc gtcgatgaag
     3241 aggatgatct tgccctccga gcgggcggtc tcctcgatga cggctttcag acgctcctcg
     3301 aactcgcccc ggtacttggc acccgcgagc aggctgccca tgtcgagctg gacgatgcgt
     3361 ttgtcctgca ggccctcagg cacgtccttg ttgacgatgc gcagcgcgag cccttcagcg
     3421 atggccgtct tgccgacgcc cggctcgccg atgaggacgg ggttgttctt cgt
//
LOCUS       DAOQYE010000097         3463 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_28419_length_3463_cov_2.488263, whole genome shotgun sequence.
ACCESSION   DAOQYE010000097 DAOQYE010000000
VERSION     DAOQYE010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 3463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3463
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28419_length_3463_cov_2.488263"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..584)
                     /locus_tag="PKN52_02545"
     CDS             complement(<1..584)
                     /locus_tag="PKN52_02545"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNQ98850.1"
                     /translation="MKRLLALVTLGAVLAFAQAADPGVTDTEVLFGSWGPQSGPAAAW
                     GTVNIAIDAYFRYLNDQGGINGRKLVLSTRDDGYDPARTVGAVRELIDRDNVFAFVAG
                     VGTANGQAAMPLIKRAGVPWVGPATGAVVFTDESDGLIFTSFTDYVVEATLMTRHAVE
                     TSTVRTGVTRPSLPRGPGPTRPSRSSPSSAPHRRG"
     gene            complement(753..1646)
                     /locus_tag="PKN52_02550"
     CDS             complement(753..1646)
                     /locus_tag="PKN52_02550"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNQ98851.1"
                     /translation="MAAVNGFDNLVQAIVSGASVGSVYALVALGLVLLYRTTRILNFA
                     HGDLGVAGTFVAYTLLTQLHLPFGVVFLLALAAAAVIGMLVYLLLVRPAKNQTELGLL
                     ILTLGLALALGGLDALVFGTDNKLVPTIVADRIIRFGGVSLSLVSIVTSLLGVVLMLA
                     LWALLSFTKLGVAMRAVAQREDVAEAMGLPVRTVLMATWAAAAILAAAAALLFAPTTL
                     LNPNMMLDPLSKGFVAAVIGGMNSLPGAVLGAYILGVLELLVGLYVSLEFKASFAFLV
                     VVLVLVLKPHGLLGRADVKRV"
     gene            complement(1634..2611)
                     /locus_tag="PKN52_02555"
     CDS             complement(1634..2611)
                     /locus_tag="PKN52_02555"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNQ98852.1"
                     /translation="MLFRERYEHELWLIRGSQAWVVAGLAGALLIALPFLVKGYLIYN
                     MTLFFVYALVALSLSVLVGLTGQVSLGHAGFLAAGAYVYVLATQHGFHYLLAVLAAVL
                     VSAVLGVVIGVPSLRLEGPYLAIATLGFGLAIQQILNNWRLVGASTGTLVDRPVLFGI
                     DFYGDREYYYLVLVLAGLVTWMLFNLERSHIGRAFRATRDADLAAQMSGIGLARYKTL
                     AFVISAAVAGLAGALYGPLVGYVTPEGFNLLLSIKFLLMTVVGGLGFLPGALIGAGFI
                     TGLDLVLSTFRSWSQLAFGTVVIGIMMLEPAGLYGRWQKIKQFWKSWPL"
     gene            complement(2674..>3463)
                     /locus_tag="PKN52_02560"
     CDS             complement(2674..>3463)
                     /locus_tag="PKN52_02560"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNQ98853.1"
                     /translation="RLLRLRLGLDRVRIAISGAAPIAPEIIAYFRAIGVDVREGFGLT
                     ESTGIIAIHRSDGRLGTVGRPFKGVEVRIAEDGEILSRGLGNFLGYHKDPVATTEALE
                     GGWLHTGDVGEIDDDGQMRITDRKKDILITAGGKNIAPQKIENQLKSSPYINDAVVIG
                     DRRKYLTALLVLDEDNVTFWAAEQQLSYSTYTDLASNPEVRELVEGEVQRVNATLARV
                     ETIKKFAILPKRLYHEDGEVTATLKVKRSSIARKYAELIEEMYA"
BASE COUNT          583 a         1122 c         1252 g          506 t
ORIGIN      
        1 ccgcggcggt gcggcgcgga cgagggggac gaacgtgacg gtcgcgtcgg tccgggcccg
       61 cgcggtaggc tcggacgggt gaccccggtt cgcacggtgc tcgtctcgac ggcgtggcgc
      121 gtcatgagcg ttgcctcgac gacgtagtcc gtgaacgacg tgaagatgag gccgtcggac
      181 tcgtccgtga agacgaccgc gccggtcgcc ggtccgaccc acggaacgcc ggcgcgcttg
      241 atgagcggca tggcggcctg gccgttggcc gtgccgacgc cggccacgaa cgcgaacacg
      301 ttgtcgcggt cgatgagctc gcgcacggcg ccgaccgtgc gggcggggtc gtaaccgtcg
      361 tcacgcgtgc tgagcacgag cttgcggccg ttgatgccgc cctggtcgtt gaggtagcgg
      421 aagtacgcgt ctatggcgat gttgacggtg ccccacgcgg cggccgggcc cgactgcggg
      481 ccccagctcc cgaagaggac ctccgtgtcg gtgacgccgg ggtcggccgc ctgggcgaac
      541 gccaggacgg cgccgagcgt gactaaggct agaagccgct tcatgttgct cctttcgtgt
      601 cccctccagg gggtggtcca gtggtctctg acgcttctca acgtatcacg cggcctagcg
      661 gcggcgttgc gggccatcga gcggttcgtg caacgtcgca ggcgaccttg tcgcgcgagc
      721 gctcgcgttg ggaggccgca tcgcccggcg gctcagacgc gcttcacgtc cgccctgccg
      781 agcagcccgt gcggcttcag caccaagacg agcaccacga ccaggaacgc gaacgacgcc
      841 ttgaactcga gggagacgta cagtccgacg agcagctcga ggaccccgag gatgtaggcg
      901 cccagcacgg cgcctggcag cgagttcatt cctccgatga cggccgcgac gaaccccttg
      961 ctcaaggggt cgagcatcat gttggggttg agaagcgtgg tgggggcgaa gaggagcgct
     1021 gctgccgccg cgaggatggc ggccgccgcc caggtggcca tgagcaccgt gcgcaccggc
     1081 aggcccatcg cctccgccac gtcctcgcgc tgcgcgaccg cccgcatggc gaccccgagc
     1141 ttcgtgaacg acaggagcgc ccagagcgcc agcatcagga cgacgcccag cagcgaggtc
     1201 acgatggaga cgaggctcaa cgagacgccg ccgaaccgga tgatccggtc ggccacgatg
     1261 gtcggaacga gcttgttgtc ggtgccgaac acgagggcgt cgaggccgcc gagggccagg
     1321 gcgaggccga gggtgaggat gagcaggcca agctccgtct ggttcttggc cggtcgcacg
     1381 aggagcaggt agacgagcat gcctatgacc gcggcggccg ccagggcgag caggaagacg
     1441 accccgaacg gcaggtggag ctgcgtgagg agcgtgtagg ccacgaacgt gcccgccacc
     1501 ccgaggtcgc cgtgcgcgaa gttgaggatg cgcgtcgtgc ggtacaggag gacgaggccg
     1561 agggcgacga gggcgtagac gctgccgacg ctggcgccgc tcacgatggc ctgcacgagg
     1621 ttgtcgaagc cgttcacagc ggccacgact tccagaactg cttgatcttc tgccagcgtc
     1681 cgtacaggcc ggcgggctcg agcatcatga tgccgatgac cacggtgccg aacgcgagct
     1741 gcgaccagga gcggaacgtg gagaggacga ggtcgaggcc ggtgatgaag cccgctccga
     1801 tgagggcgcc gggcaggaag ccgagcccgc ccacgaccgt catgagcagg aacttgatgg
     1861 agagcaggag gttgaagccc tcgggcgtca cgtagccgac cagcgggccg tacagcgcgc
     1921 cggccagccc ggccacggcg gccgagatga cgaacgccag cgtcttgtag cgcgccagtc
     1981 cgatgccgct catctgcgcg gccaggtcgg cgtcgcgcgt cgcccggaag gcccgcccta
     2041 tgtgcgagcg ctcgaggttg aagagcatcc acgtcaccag accggccaac acgagcacga
     2101 ggtagtagta ctcgcggtcg ccgtagaagt cgatgccgaa caggacggga cggtcgacga
     2161 gcgtgccggt agaggcgccc accagtcgcc agttgttgag gatctgctgg atggcgagac
     2221 cgaagcccaa ggtggcgatg gccaggtacg gcccctcgag gcgcaaggac ggcaccccga
     2281 tgacgacccc gaggacggcc gacaccagca cggccgccag gacggccagc aggtagtgga
     2341 agccgtgctg cgttgccagc acgtagacgt acgctccggc ggccaggaag ccggcgtgtc
     2401 cgagcgacac ctggccggtg agcccgacga gtaccgacag cgacagcgcc acgagcgcgt
     2461 acacgaagaa gagggtcatg ttgtagatca ggtagccctt gaccaggaac ggcagcgcga
     2521 tgaggagcgc gcccgccagc ccggcgacca cccaggcctg cgagccgcgg atgagccaca
     2581 gctcgtgctc gtagcgctca cggaagagca tggcggcccc agtcggtgtc cgtcgcggtg
     2641 ccctggtccg gacgctggcg ggctgggcga ctgtcaggca tacatctcct cgatcagctc
     2701 ggcgtacttg cgggcgatgc tcgagcgctt caccttgagc gtggccgtga cctcgccgtc
     2761 ctcgtggtag agccgcttgg gcaggatggc gaacttcttg atggtctcga cccgggccag
     2821 cgtggcgttg acacgttgca cctcgccctc gactagctcg cgcacctccg ggttgctcgc
     2881 caggtccgta taggtcgagt acgagagctg ctgctcggct gcccagaacg tgacgttgtc
     2941 ctcgtcgagc acgagcagcg ccgtgaggta cttgcggcga tcgccgatga ccacggcgtc
     3001 gttgatgtaa ggcgacgact tcagctggtt ctcgatcttc tgcggggcga tgttcttgcc
     3061 gccggcggtg atgaggatgt ccttcttacg gtccgtgatg cgcatctggc cgtcatcgtc
     3121 gatctcgccg acgtcgcccg tatgcaacca gccgccctcc agcgcttcgg tcgtcgcgac
     3181 cggatccttg tggtacccga ggaagttgcc taggccgcgg ctcaggatct cgccgtcctc
     3241 cgcgatcctc acctcgacgc ccttgaacgg gcgcccaacg gttccgagcc gcccgtcgct
     3301 gcggtggatg gcgatgatgc cggtcgattc cgttaggccg aagccctcgc gcacgtccac
     3361 gccgatggcc cggaagtagg cgatgatctc tggcgcgata ggggccgcgc cggagatggc
     3421 gatgcggacg cggtcgaggc cgaggcgcag ccgcaggagc ctt
//
LOCUS       DAOQYE010000098         3451 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_28564_length_3451_cov_2.803298, whole genome shotgun sequence.
ACCESSION   DAOQYE010000098 DAOQYE010000000
VERSION     DAOQYE010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 3451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3451
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28564_length_3451_cov_2.803298"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="PKN52_02565"
     CDS             <1..304
                     /locus_tag="PKN52_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HNQ98854.1"
                     /translation="TIHATTHESPTYVVDGVVHYGVANMPGAVPNTSTRALSNQTLTY
                     ALRLASKGLSALQDDAAFLKGLNTHAGAITYGAVGQAFDLPVVDPASALRAGGVPA"
     gene            complement(315..920)
                     /gene="ruvA"
                     /locus_tag="PKN52_02570"
     CDS             complement(315..920)
                     /gene="ruvA"
                     /locus_tag="PKN52_02570"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HNQ98855.1"
                     /translation="MIAFVDGVVAAVGETSVVVAVGGFGLEVLAPSSTLARCAVGSEI
                     RLRTHLVVREEQPSLYGFHQAEQLALFKRLLEVGGVGPKLALGVLSSLNASLIVTAVM
                     NDDPGLLTSAPGVGKRTAERIVLELKGRLPDDLLALVGDGKSVAGARGGIGAAGEDAV
                     AALLALGYREGQVRAAVADLLAKAPADTAEALIRKALSQLR"
     gene            complement(917..1696)
                     /locus_tag="PKN52_02575"
     CDS             complement(917..1696)
                     /locus_tag="PKN52_02575"
                     /inference="COORDINATES: protein motif:HMM:NF013253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HNQ98856.1"
                     /translation="MTQRELAALDAYQLLPRALHDVANPDMTAAVLGLTLAAPIVPRR
                     RRGDALASGQLAALPAEAVLADIERHPAGHTVAVLPPRAMSELVGQVKRLAARGVAAV
                     GLDLAPLADTAPFGTEEWRPRTREDLAELRAAAGCPLWLFGVASAADAEVAAEAGAEA
                     VVVSGGLGAHVAAPPVIELLPEIVDATGGMLTIASGGPVRDGLDVLRYLAVGAEIVVM
                     EGDRDPVDIAAELAYAMRLTACANLGDIGYDLLFAPAFEEP"
     gene            1813..2565
                     /locus_tag="PKN52_02580"
     CDS             1813..2565
                     /locus_tag="PKN52_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNQ98857.1"
                     /translation="MNELVSVRGISKAFGTVKAVDDLTFDVAASEVFGVLGPNGAGKT
                     TTLRIIATLLAPDAGTATVAGHDVVKDAERVRATIGVVNGGMGLYDRLTGREILHYFG
                     GLYGMRSAAIEARIAELDQLLQLGDTLSRRAGGFSTGMKQKVVIARAVLHDPQVIFFD
                     EATSGLDVVARRAVIDFVKAYPSAGRAVVYSTHVMSEVEELCDRACIIYRGRKIAEGT
                     IAELAAQGDGKGLEEAFFRLVNRYDTSRGVAA"
     gene            2562..>3451
                     /locus_tag="PKN52_02585"
     CDS             2562..>3451
                     /locus_tag="PKN52_02585"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNQ98858.1"
                     /translation="MRPRIILSLARKEIISTLRDTRAIVSNLVIPLVLVPVMMIGMPL
                     LLGSLFEREQVTVTNVGVVGEANVPQELRAAFTAANLALEPVTDADQAVRDGTVDVAV
                     VIPGGFEAMLASGGSATVEVHSKVGNMKSELNTSKVQQAFSDYQSRVVAGRLSAAGLD
                     ASVLRPVTVRTIDASSQAERSSGQLSWLIPFFIAIWTLTGGQMTAIDATAGEKERGTL
                     EVLLVAPVRRAEVVAGKFIATMTFGLSAALMAILGIVIGGAVMGSLIVPRLGEGASGM
                     VNIMGGSIALTPTIVLALIV"
BASE COUNT          533 a         1243 c         1194 g          481 t
ORIGIN      
        1 gacgatccac gccaccaccc acgagagccc gacctacgta gtcgacggcg tcgtgcacta
       61 cggggtcgcc aacatgccgg gcgccgtgcc gaacacgagc acccgcgccc tctcgaacca
      121 gacgctgacc tacgccctgc gcctcgcgag caagggcctg agcgcgctcc aggacgacgc
      181 ggcgttcctc aagggtctca acacgcatgc gggggccatc acgtacggag ccgtcgggca
      241 ggctttcgac ctgccggtcg tcgaccctgc cagcgcccta cgcgccggcg gcgtgccggc
      301 ctgaggagaa cgcgtcagcg gagctgcgac aacgctttgc ggatgagcgc ctccgcggtg
      361 tcggccggcg ccttggccaa gaggtcggcg accgccgcgc gcacctgccc ctcgcggtag
      421 cccagggcga ggagcgccgc cacggcgtcc tctcccgcgg ccccgatacc gcccctcgct
      481 ccggcgacgg acttgccgtc gcccaccagg gcgagcaggt cgtccgggag tcggcccttc
      541 agctccagga cgatgcgctc ggccgtgcgc ttgccgaccc cgggcgcgct ggtcagcagc
      601 ccggggtcgt cgttcatgac ggcggtcacg atgagcgagg cgttcaggct ggacaggacc
      661 ccgagggcga gcttcgggcc gacgccgcca acctcgagca ggcgcttgaa cagggcgagc
      721 tgctcggcct ggtggaagcc gtataggctg ggctgctctt ccctgacgac gaggtgggtg
      781 cgcaacctta tctcgctccc gacggcgcac ctcgccagag tggaactcgg cgccagcacc
      841 tcgagtccga agccgccgac cgcgacgacc acgctggtct cgccgaccgc ggccaccacc
      901 ccgtcgacga aggcgatcac ggttcctcga aggcgggggc gaagaggaga tcgtaaccga
      961 tgtcgcccag gttggcgcag gccgtgagcc gcatagcgta cgcgagttcc gcggcgatgt
     1021 cgaccgggtc gcggtcgccc tccatcacga cgatctcggc gccgaccgcc aggtagcgca
     1081 agacgtcgag cccgtcgcgc acaggacccc cggaggcgat cgtgagcatg ccgccggtgg
     1141 cgtccacgat ctcaggcagc aactcgatga cgggcggggc cgccacgtgc gccccgaggc
     1201 caccgctcac gaccaccgct tcggcgccgg cctcggcggc cacctcggcg tcggcggccg
     1261 aggccacacc gaagagccag agcggacacc ctgcggcagc ccggagttcg gcgaggtcct
     1321 ccctggtgcg cgggcgccac tcttccgtgc cgaacggggc ggtgtcggcg agcggcgcca
     1381 ggtcgagtcc gacggcggcc actccgcgcg ccgccaggcg cttcacctgg ccaacgagct
     1441 ctgacatggc tcggggcggc agcacggcga ccgtatgccc cgcggggtga cgttctatgt
     1501 cggcgagcac cgcctcggcg ggcaatgcgg ccagttggcc gctcgccaac gcgtccccgc
     1561 ggcggcgcct cggcacgatg ggggcggcga gggtgaggcc gaggacggct gcggtcatgt
     1621 cggggttggc gacgtcgtgc agcgcccgcg gcaggagctg gtacgcgtcc aacgcggcta
     1681 actcgcgctg tgtcacacct ggatgatagc aaggcccgtc gagtgccggc tcggcgaccc
     1741 gggcagttcc ggaacgcccg ggcagctttc gggcgtccgg gcctcacgga ggcgtctgct
     1801 aacatcgccg gcgtgaacga actcgtctcc gtccgcggca tcagcaaggc gttcggcacc
     1861 gtcaaggctg tcgacgacct gacgttcgac gtcgcggcat cagaagtctt cggcgtcctg
     1921 gggcccaacg gggccggtaa gacgaccacc ctcaggatca tcgccacgct gctcgcgccg
     1981 gacgccggca ccgccaccgt cgccggccat gacgtggtga aggacgccga gcgcgtacgc
     2041 gcgacgatcg gcgtggtgaa cggcggcatg ggcctctacg acaggctcac cggacgcgag
     2101 atcctccatt acttcggcgg actctacggc atgcgcagcg cggccatcga ggcgcgcatc
     2161 gcggaactcg accaactgct gcagctcggc gacacgctgt cgcgccgcgc cggcgggttc
     2221 tcgaccggca tgaagcagaa ggtcgtcatc gcgagggccg tcttgcacga cccgcaggtc
     2281 atcttcttcg acgaggccac gagcgggctt gacgtcgtgg cgcgccgcgc cgtcatcgac
     2341 ttcgtcaagg cgtacccgag cgccgggcgc gccgtcgtct actcgactca cgtcatgagc
     2401 gaggtcgagg agctctgcga ccgcgcctgc atcatctacc ggggccgcaa gatcgccgag
     2461 ggcaccatcg ccgagctcgc ggcgcagggc gacggcaagg gtctcgagga ggccttcttc
     2521 cgcctcgtga accgttacga cacgtcgcgg ggggtggccg catgagaccc cgcatcatcc
     2581 tgagcctcgc ccgcaaggag atcatctcca cgctgcgcga cacgcgggcc atcgtctcca
     2641 acctcgtcat cccgctcgtg ctcgttcccg tcatgatgat cggcatgccg ctcctcctcg
     2701 gcagcctctt cgagcgcgag caggtcaccg tgacgaacgt cggcgtcgtc ggcgaggcga
     2761 acgtccccca ggagttgcgc gccgccttca cggccgccaa cctcgcgctc gagccggtca
     2821 cggacgccga ccaggccgtg cgcgatggca ccgtggacgt ggccgtcgtc ataccgggcg
     2881 gcttcgaggc gatgttggct agcggcggca gcgccaccgt cgaggtgcac agcaaggtcg
     2941 ggaacatgaa gagcgagctg aacacctcga aggtccagca ggccttctcc gactaccagt
     3001 ccagggtcgt ggccgggcgc ctgagcgcgg ccgggctcga cgcgagcgtc ctccgacccg
     3061 tgaccgtccg gacgatcgac gccagcagcc aggccgagcg cagcagcggg caactctctt
     3121 ggctcatccc gttcttcata gccatctgga cgttgacggg tgggcagatg acggccatag
     3181 acgccacggc cggcgagaaa gagcgcggca cgctcgaggt cctgctcgtg gcgccggtac
     3241 gccgcgccga ggtcgtggcg ggcaagttca tcgcgacgat gaccttcggt ctcagcgccg
     3301 ccctgatggc catcctcggc atcgtcatcg gcggcgccgt gatgggcagc ctcatcgtgc
     3361 cgcgcctcgg cgagggcgcg agcggcatgg tcaacatcat gggtggcagc atcgccctga
     3421 cgccgaccat cgtcctcgcg ctcatcgtga g
//
LOCUS       DAOQYE010000099         3450 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_28576_length_3450_cov_3.453903, whole genome shotgun sequence.
ACCESSION   DAOQYE010000099 DAOQYE010000000
VERSION     DAOQYE010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 3450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3450
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28576_length_3450_cov_3.453903"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(56..658)
                     /gene="lspA"
                     /locus_tag="PKN52_02590"
     CDS             complement(56..658)
                     /gene="lspA"
                     /locus_tag="PKN52_02590"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HNQ98859.1"
                     /translation="MLLLAAALVALDQLSKAWVVATLPRDGTEIPLALGFGITHTRNG
                     GAAFGMLQDLELKVGGLTIDGTVLLGLLSAVVSVGIGVYLWRNAKRLPALLRVTLALV
                     LAGAAGNMLDRLRLGYVVDFVHFKVGSFAFPVFNVADACVVIGAILLFIGSLGGEPRG
                     DTRQDVKRAPAPREHRLDELPEVPPLGAGKQQGDASNSNT"
     gene            1032..>3450
                     /locus_tag="PKN52_02595"
     CDS             1032..>3450
                     /locus_tag="PKN52_02595"
                     /inference="COORDINATES: protein motif:HMM:NF016944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HNQ98860.1"
                     /translation="MSVTLEQYLATLRAAGDPRLERFATALYEKADAGLIALLGPDAL
                     TALARKGLEFLGQLGNAPVAVAAFGANDVGSAAPLPYSVLMLALKDRPFIVDSVQAAL
                     RRRGLPVADKLHPTVLVSRDAHGALVDLGQGLATAGTRREAFEIYLLDAGLDAQTLGA
                     AVDGVREVLGDVILATDDYRAMLARSRAVADYLRGLDGVQRAAAARFGATDSNEDAAF
                     LEWLEDDNFVFLGYREYEIVSPAGRPALGLVKDSGLGVLRKVASSAYSTPVPLEELPE
                     ALRERVSGGRFFLVTKANSEATVHRARRMDYIGVKRLSDTGAVVGEQRFVGLFTTKGL
                     AAPVEDVPILRSKLRSVLERDQAIPGSHDYKATVAAFNGMPREELFWMSVDQIHADIR
                     TILRVGQDPTARLTLHEDPLGRGLAAMVVMPRESFSDDVRRAVQSYLTEALGAKHFDS
                     RLALGEDEEHARFHFYFVTDKRLADIDVKAVERQVQRLSRSWSEALRELLVAAHGENA
                     GRALSARYAQVFDERFRATVAPEQAIADIDHLERLAERQRVVDLAPVPASSGTADAAQ
                     ALLRVYHRGHGLALSDVLPLLENVGLRVIEQYPYNLMVEGESRGVDVFTVVNATGGEF
                     DVEAEKGRLVEALEGLLAGEVENDELNRLVLRARLSARQVALLRAYQMYYAQVNLVTS
                     RSFVSAALLAHPRVAALLMRYFETRFDPGFAGGAGAYSAEREAAAQSAHQAVIDALGE
                     VSSLAEDGALRGLLDLMAATVRTSYFLGFERISFKLDSAKVGAMPEPRPMFEIAVSAP
                     QVEGTHLR"
BASE COUNT          531 a         1227 c         1179 g          513 t
ORIGIN      
        1 ggcgccgtgc gacgacggct ccgccagcgt gatctggacg ccgaagaggc gccgctcagg
       61 tgttggagtt cgaagcgtcg ccctgctgct tacccgcacc tagcggcggt acctccggca
      121 gctcgtcgag acgatgctca cgcggcgccg gcgcgcgttt gacgtcctgg cgcgtgtcgc
      181 cgcgcggctc gccgccgagg ctcccgatga agaggaggat ggcgccgatg accacgcacg
      241 cgtcggccac gttgaacacc gggaaggcga agctgcccac cttgaagtgc acgaagtcga
      301 cgacgtagcc gaggcgcaac cggtcgagca tgttgccggc ggcgcccgcg aggacgagcg
      361 cgagcgtcac cctgaggagc gcggggagcc gcttggcgtt gcgccacagg tagacgccga
      421 tgccgacgga caccacggca gagagcaggc cgaggagcac ggtgccgtcg atggtcagcc
      481 ctccgacctt gagctcgagg tcttgcagca tgccgaaagc cgcgccgccg ttacgcgtgt
      541 gggtgatccc gaaaccgagc gccaaaggga tctcggtacc gtcgcgcggc agggtagcca
      601 ccacccaagc cttgctcaac tgatctagcg cgaccaaggc ggccgcgagg agaagcatca
      661 cccgagcagt atagtccgcg ctccttggcg ggaaacggtc ggtcgtgcgc tcgcagtgcg
      721 cgggggaccg cgccgccgcc cggctgctcc cccacacggc ccgttgacaa ggtgggcggc
      781 cgtgaccgag cgccgttgct cctctacgta agccgcaggg gacgccctag catgcgtaag
      841 ctgcagggga ctccctcgca agcggtgcac agagctcctc ccacgtctca agtggaggag
      901 atggggttgt ttcgctcgct ctccaagggc gcgtgcccta gcatcggttc ggcggtatct
      961 tggccctgac tacacgacta cacggggcgc ggcgccaagc gcgtcgcacg gcccgcggaa
     1021 ggcagacgag catgtccgta accttggaac agtacctggc aaccttgcga gccgccggcg
     1081 acccgcgcct cgagcgcttc gcgaccgcgc tctacgagaa ggccgacgcc ggactcatag
     1141 cgctcctcgg tcccgacgcg ctgacggcgc ttgcgaggaa gggtctcgag ttcctcggcc
     1201 agctcggcaa cgccccagtc gccgtcgccg cgttcggcgc gaacgacgtt gggtcggccg
     1261 cgcctctgcc gtactcggtc ctcatgctgg cgctcaagga ccggccgttc atcgtcgact
     1321 cggtgcaggc ggcgttgcgc aggcgcgggt tgccggtggc cgacaagctc cacccgaccg
     1381 tgctggtgag ccgcgacgcg cacggggcgc tcgtcgacct cggacagggg ctcgccaccg
     1441 ccggcactag gcgcgaggcc ttcgagatct acctgctcga cgccggcctc gacgcgcaga
     1501 cgcttggcgc ggcggtcgac ggcgtcaggg aggtgctcgg tgacgtcatc ctcgcgactg
     1561 acgactaccg ggccatgctc gcccggagcc gcgccgtcgc cgactacctg cgggggctgg
     1621 acggcgtgca gcgcgccgcc gcggcccggt tcggtgccac ggactccaac gaggatgccg
     1681 ccttcttgga atggctcgag gacgacaact tcgtgttcct gggctaccgg gagtacgaga
     1741 tcgtctcacc ggccgggcga cccgctctcg gtctcgtcaa ggacagcggc ctcggagtct
     1801 tgcgcaaggt cgccagcagc gcctacagca cgcccgttcc actcgaggag ctacctgaag
     1861 ccctgcgcga acgcgtgtct ggcggtcgct tcttcctagt caccaaggcg aactcggaag
     1921 ccacggtgca ccgtgcgagg cgcatggact acatcggcgt caagcgcctc tccgacaccg
     1981 gcgcggtggt gggcgagcag cggttcgtcg gcctcttcac gaccaagggt ctggcggcgc
     2041 ccgtcgagga cgtgcccatc ttgcgcagca agctgcgcag cgtgctcgag cgcgaccagg
     2101 ccatccccgg ctcccacgat tacaaggcga cggtcgccgc cttcaacggc atgccgcgcg
     2161 aagagctctt ctggatgagc gtcgaccaga tccacgccga catccgcacg atcctgcgcg
     2221 tcggtcagga cccgacggcg cgcctcaccc tgcatgagga cccgctcggc cgcggtctcg
     2281 ccgccatggt cgtcatgccg cgcgagagct tctccgacga cgtgcgcagg gccgtgcaga
     2341 gctacctgac cgaggcgctg ggagccaagc acttcgactc gcggctcgcc ctcggcgagg
     2401 acgaagagca cgcccgtttc cacttctact tcgtgacgga caagcgcctc gcggacatcg
     2461 acgtcaaggc cgtcgagcgt caggtccagc gcctgtcgcg ctcctggtcg gaggcgttgc
     2521 gcgagctcct cgtcgcggcg cacggcgaga acgcggggcg cgccctctcc gcgcgttacg
     2581 cccaggtgtt cgatgagcgt ttcagggcca cggtagcgcc tgagcaggcg atcgccgata
     2641 tcgaccacct ggagcgtctc gccgagcggc agagggtggt cgacctcgct ccggtaccgg
     2701 cgagcagcgg caccgcggac gccgcccagg cgctcctccg ggtctaccac cgcgggcacg
     2761 ggctcgcgct ctccgacgtg ctgcccctgt tggagaacgt cggcctcagg gtcatcgagc
     2821 agtacccgta caacctgatg gtcgaaggcg agtcccgcgg agtcgacgtc ttcacggtcg
     2881 tcaacgccac cggcggcgag ttcgacgtcg aggctgagaa gggccggctc gtcgaggcgc
     2941 tcgaggggct cctggcgggc gaggtcgaga acgacgagtt gaaccggctc gtgctgcggg
     3001 cgcggctcag cgccaggcag gtcgccctcc tgcgggccta ccagatgtac tacgcgcaag
     3061 tgaacctggt cacgagtcgc tcgttcgtga gcgccgccct cctcgcccac ccgcgcgtcg
     3121 ccgcgctcct catgcgctac ttcgagaccc gtttcgaccc cggcttcgcc ggcggtgccg
     3181 gcgcctactc cgccgaacgc gaggcggcgg cgcagagcgc gcaccaggcc gtcatcgacg
     3241 ccctcggcga ggtctcctcg ctggccgagg acggcgcgct ccgcggcctc ctcgacctca
     3301 tggccgccac cgtgcgcacc agctacttcc taggcttcga gcgcatcagc ttcaagctcg
     3361 actctgccaa ggtcggcgcc atgcccgagc cacggcccat gttcgagatc gccgtatcgg
     3421 cgccgcaggt ggaaggcacg cacttgcggg
//
LOCUS       DAOQYE010000100         3449 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_28588_length_3449_cov_2.740130, whole genome shotgun sequence.
ACCESSION   DAOQYE010000100 DAOQYE010000000
VERSION     DAOQYE010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 3449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3449
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28588_length_3449_cov_2.740130"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1222)
                     /locus_tag="PKN52_02600"
     CDS             complement(<1..1222)
                     /locus_tag="PKN52_02600"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HNQ98861.1"
                     /translation="MFEPITSLTAAELARRYRDGSLDPVTVTEAYLAAIEAHPEGAKV
                     YRVVTAQRALKQARAAKRRFEEGLHVSPMQGVPIAIKDLIGTAGDVTAAGSKVLAERP
                     AEAEDAPVAARLDAAGAVFLGKTNMTELAYSGIGANPHYGTPGCALDPSRIPGGSSSG
                     SGVAVAARLAAVALGSDTGGSVRIPAAVNGIVGLKVTDGRIPTDGCAALSTTLDTIGP
                     LARTAEDAWALYLALTAEPHRPLASAPRRLTFLAPTTLLADDLDDATRSGLEEGLARL
                     VDLGHEVRREPLPILLEAPAAYARYGSFASHEVWALYKELFEGRRDDFDPHVADRVYL
                     YAGKPSSDYIRLCYARADLSRRFWPSLAGVDALIGPTIPHVPVEIAAVLSDDETYHRE
                     NNRILRNTAPFNVLG"
     gene            1281..1571
                     /locus_tag="PKN52_02605"
     CDS             1281..1571
                     /locus_tag="PKN52_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98862.1"
                     /translation="MQRNRAELEAMSHEDLVNRVLELQDMLREGLAVRASLHAVLNTV
                     LNAKSDEVARYAEASESALDPEELELKRAWAEARHAVSNPLGVARKRSQAVG"
     gene            complement(1607..2107)
                     /locus_tag="PKN52_02610"
     CDS             complement(1607..2107)
                     /locus_tag="PKN52_02610"
                     /inference="COORDINATES: protein motif:HMM:NF020496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HNQ98863.1"
                     /translation="MTAASAATYTFEAALIRPEGTGTWTYVEVPLDVSRALGARGRVA
                     VVCTVTGASASVEFKGSLMARGGGRHFLVVAKPIRDEAGATTGDAVTVAVRRDTSERV
                     VEVPQELAAALDENPAARDVYEGYSYSHRKEYATWVGEAKKPETRAARAAKAVAMLAE
                     GKRLKA"
     gene            complement(2250..2324)
                     /locus_tag="PKN52_02615"
     tRNA            complement(2250..2324)
                     /locus_tag="PKN52_02615"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2290..2292),aa:Asn,seq:gtt)
     gene            2458..2808
                     /locus_tag="PKN52_02620"
     CDS             2458..2808
                     /locus_tag="PKN52_02620"
                     /inference="COORDINATES: protein motif:HMM:NF015758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase
                     family protein"
                     /protein_id="HNQ98864.1"
                     /translation="MTEALTLDAYQTGAKSTALYSPDVRVLYPVLKLAGEAGEVAEKL
                     GKFMRDEGYRPGDALNDEQREKMVKELGDVLWYVANLAADLGVSLEHVARVNLEKLQS
                     RKARGVIGGSGDDR"
     gene            complement(2780..3136)
                     /locus_tag="PKN52_02625"
     CDS             complement(2780..3136)
                     /locus_tag="PKN52_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010512344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HNQ98865.1"
                     /translation="MVRIKRVYEPAADSDGARVLVDRVWPRGVSKEEADIVWWAKDVA
                     PSTELRKWFGHDPARWTEFQKRYRQELVGNAALQKLEGMVVKGDVTLVYGAKDEEHNQ
                     ARVLLELLTDRPRSPR"
     gene            complement(3175..>3449)
                     /locus_tag="PKN52_02630"
     CDS             complement(3175..>3449)
                     /locus_tag="PKN52_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98866.1"
                     /translation="ALGRVVSIALQYGVPAEALVKTLRGINGGMYGTYHGRMVASKAD
                     LLAVALETVGVKNVLNHGKACPDCGAPLRFEEGCSKCENCGFSKCG"
BASE COUNT          512 a         1166 c         1221 g          550 t
ORIGIN      
        1 taccaaggac gttgaagggc gccgtgttgc gcaggatgcg gttgttctcc ctatggtagg
       61 tctcatcgtc cgagaggacc gccgcgatct cgaccggcac gtgcgggatc gtcggtccta
      121 tgagcgcgtc gacgccggca agcgacggcc agaagcgccg gctgaggtcc gcgcgcgcgt
      181 agcagaggcg gatgtagtcg gaggacggct tgcccgcgta caggtagacg cggtcggcga
      241 catgcgggtc gaagtcgtcc ctgcgcccct cgaagagctc cttgtagagc gcccacacct
      301 catggctggc gaaagagccg taacgcgcgt aggcggcggg cgcctctagg aggatcggga
      361 gcggctcgcg cctcacctcg tgccctaggt ccaccagccg cgcgagaccc tcctcgaggc
      421 cgctgcgcgt cgcatcgtcg aggtcgtcgg ccagcagtgt ggtgggagcc aggaacgtga
      481 gtctgcgggg cgccgaagca agtggacgat gcggctcggc ggtcagcgcg aggtagagcg
      541 cccaggcgtc ctctgccgtg cgcgccaacg gtccgatggt atcgagggtc gtcgagagtg
      601 cggcgcagcc gtcggtcggg atgcgtccgt ccgtcacctt caggccgacg atcccgttga
      661 cggcggcggg tatgcgcacg ctgccgcccg tgtctgaacc gagggccacg gccgccaggc
      721 gcgccgcgac cgccacaccc gagccggatg acgagcctcc cgggatgcgg ctcgggtcga
      781 gcgcgcatcc gggcgtaccg tagtgcgggt tggcgccgat accagagtag gcgagctcgg
      841 tcatgttcgt cttgccgagg aacacggcgc cggccgcgtc gagccgggcc gccaccggcg
      901 cgtcctcggc ctcggccggg cgttctgcca gcaccttcga gccggccgcc gtgacgtcgc
      961 cggccgtgcc gatcaggtcc ttgatcgcga tgggcacgcc ctgcatgggg ctcacgtgca
     1021 gcccttcctc gaagcggcgt ttggccgccc tggcctgctt gagcgcccgc tgggccgtca
     1081 cgacccggta gaccttggca ccttccgggt gggcctcgat ggcggccagg taggcctccg
     1141 tgacggtgac cgggtcgagc gagccgtcgc ggtaacgccg agcgagctcg gctgccgtca
     1201 gggacgtgat cggttcgaac atgccgcaca cgataccgcg gtaggccacg tggtctttat
     1261 ggcgcggtac gatgcctgcc atgcagcgca accgcgcgga actcgaagcc atgagccatg
     1321 aggacctcgt caaccgcgtc ctcgagcttc aggacatgct gcgcgagggc ctggccgtgc
     1381 gtgcttccct gcacgccgtc ctgaacacgg tgctcaacgc caagagcgac gaggtggccc
     1441 gttacgccga ggcctccgag tccgcgctcg acccggagga gcttgaactc aagcgcgcgt
     1501 gggccgaggc gcgccacgcc gtctccaacc cgctcggcgt cgcgcgcaag cgcagccagg
     1561 cggtcggctg aggagccgcg ggccgagtgg tatcggggcg gacccgttac gccttcagcc
     1621 tcttcccctc cgcgagcatc gccacggcct tggccgcgcg ggctgcgcgc gtctccggct
     1681 tcttcgcctc gccgacccag gtcgcgtact ccttgcggtg cgagtacgag tagccctcgt
     1741 agacgtcgcg cgcggccggg ttctcgtcca gcgcggcggc cagctcctgc ggaacctcga
     1801 cgacacgctc cgacgtgtcc cgccgaacgg cgacggtcac ggcgtctccg gtcgtcgctc
     1861 cggcctcgtc gcggatgggc ttggcgacga cgaggaagtg ccgccctccg ccccgcgcca
     1921 tcagcgaacc cttgaactcg acgctagcgc tggcgcccgt gacggtgcag acgacggcga
     1981 cgcgcccccg cgcgccgagc gctcggctca cgtcgagcgg tacctctacg taggtccagg
     2041 taccggtccc ttcggggcgg atcagcgcgg cttcgaaggt gtaggtggcg gcgctcgctg
     2101 cggtcatgtt gggggacgtt agcacgctcg ccgagccgac gggctccgaa ctccgcgcgg
     2161 gggtactttc cggggatacg agccgttacc gccgcgtgaa ccgaagaagc ccgaccccgc
     2221 gatctctcgt ctgggtcggg cttctcgctt ggctcggcgg gtaggatttg aacctacgac
     2281 caatcggtta acagccgatc gctctgccgc tgagctaccg ccgaacgcgc gcttcggccg
     2341 ccaggaccgg aaagcgaagt gcagtgtacc agggggcgcg ggagttggca acatgcgtct
     2401 ggcacgctct cgccggcccg gcgcgtctgg ggagcgggat tcggttagca tccggtcatg
     2461 acggaagcgc tgacgctaga cgcttatcag acgggtgcca agtccaccgc gctgtactcg
     2521 ccggacgtac gcgtcctgta cccggtgctc aagctcgcgg gcgaggccgg cgaggtggcc
     2581 gagaagctcg gcaagttcat gcgcgacgag ggttaccgac cgggggacgc gttgaacgac
     2641 gagcaacgcg agaagatggt caaggaactc ggcgacgtgc tctggtacgt ggccaacctg
     2701 gcggccgacc ttggcgtgag cctcgagcac gtggcgcggg tcaacctgga gaagctccag
     2761 agccggaagg cgcgcggggt catcgggggg agcggggacg atcggtgagc agttccagta
     2821 ggacgcgcgc ctggttgtgc tcctcgtcct tggcaccgta gacgagcgtc acgtcgccct
     2881 tgacgaccat cccctcgagc ttctgtaacg cggcgttgcc gacgagttcc tggcgataac
     2941 gtttctggaa ctcggtccag cgcgcggggt cgtgaccgaa ccatttgcgt agctcggtgc
     3001 tcggggccac gtctttcgcc caccagacga tgtcggcctc ttccttgctg acgccgcgcg
     3061 gccagacgcg gtcgacgaga accctggcgc catcactgtc ggcggcgggt tcgtagaccc
     3121 gcttgatcct gaccatggtg ccagcatagc cccgacgacg ctcctagctg tggctcaccc
     3181 gcacttactg aagccgcagt tctcgcactt cgagcagccc tcctcgaagc gtagcggggc
     3241 gccgcagtca gggcaggcct tgccgtggtt gaggacgttc ttgaccccga cggtctccag
     3301 cgcgacggcg agcaggtcgg ccttggaggc caccatgcgg ccgtggtagg tgccgtacat
     3361 cccgccgttg atgccgcgca gcgtcttgac gagcgcctcg gccggcacgc cgtactgcaa
     3421 ggcgatcgag acgacgcggc cgagcgcct
//
LOCUS       DAOQYE010000101         3432 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_28776_length_3432_cov_3.535683, whole genome shotgun sequence.
ACCESSION   DAOQYE010000101 DAOQYE010000000
VERSION     DAOQYE010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 3432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3432
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28776_length_3432_cov_3.535683"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..740)
                     /locus_tag="PKN52_02635"
     CDS             complement(<1..740)
                     /locus_tag="PKN52_02635"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ98867.1"
                     /translation="MSRAARVFATPLGHISAAIVATYVLAAALAPVLAPYGPEAGDLA
                     QRLAPPSAQHWFGTDSLGRDILTRTLYGARVSLLVGVLTVAITAAFGTVIGAVGGFYR
                     GAFDAVVGRVTELLLAFPYLIFAIGMMAFLGPGFANLVAALSLKGWVEFYRLARGETL
                     DQAQREYVEASRAIGQRRPRTLFREVLPNVLPAVSVLSTLRIGYFMVLEASLSFLGVG
                     IPPNIPAWGSMISEGRGVLFIAWWVSTIP"
     gene            complement(737..1744)
                     /locus_tag="PKN52_02640"
     CDS             complement(737..1744)
                     /locus_tag="PKN52_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ98868.1"
                     /translation="MRLTKLLERVLSAIPVALGVAVLAFMFLRFLPGDPVEIMLGDTQ
                     VSQQQLDSLRSQLHLDKPLLTQLGIFLSGLLRGDLGVSIVKNAPVAKLIGDALPATIE
                     LTLATVLVGILIAMPVGIISAVRAGSWLDRTVLSGALVGISMPAFWFGLLMILLFAVE
                     LRVLPTSGRIAATMVVDRTTGFMLVDTLLAGNLKAFGTALKHLVLPALTLGVVFAAVL
                     ARVVRTSMLEVLGKDYVTTARAKGLREWGVILKHALRNALIPALTVTGLQFGELLGGN
                     MIVETVFAWPGLGRLVVNSIFARDYVVVQAAVMLYALTYVGANLVVDTLYTLLNPRIS
                     L"
     gene            complement(1869..3380)
                     /locus_tag="PKN52_02645"
     CDS             complement(1869..3380)
                     /locus_tag="PKN52_02645"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNQ98869.1"
                     /translation="MHRMKWLLISLAALVFMSAGAEKLTFVLDVTENRSDEGQAIVAQ
                     LQAIGIDASLRVWQTTVLRDEMLAGNRSASTSDWGSAYFDPFDLAIPKLRTKDRGNYS
                     FYSNPSVDEAFRIASTSIDDATRNEAYLTAQRQIFADAPLVFGYVLQNIEAASDSVRG
                     WEPAADNSENFARVSVAGSDTLVIGMRVDAVVTLDPAMYRDRDTEAVLRNIFDALTIP
                     TSEGQVQPHLATAWRVVDPLTYEFDLRSGVTFSNGEVLDADDVVFTFERILTEGAIDG
                     ATSPRAGLLGPIDHVEKVDATTVRFVYKETFPQELVLQALTHFQIVPKDYLTEVGVAA
                     FTAKPIGSGPFVYARGALDSQVVLEANPTHWNGAPELKSVVWQMMPEASTRIAALLAG
                     EVNVIQAVPPDLVDRIASVPGVSVRTALGTRSYQIELNNEAAPFNDTRVRQALNYAID
                     WDSILKNIYRGYADRLSTVFLPSGFGYAQDLAPYPYDPAKAKELLQQAGYETN"
BASE COUNT          612 a         1071 c         1199 g          550 t
ORIGIN      
        1 gggatcgtgc ttacccacca ggcgatgaac agcaccccgc gcccttcgga gatcatcgag
       61 ccccaggccg ggatgttggg cgggatgccg acgccaagga aactgagcga tgcctcgagg
      121 accatgaagt agccgatccg cagcgtgcta aggacgctta cggcaggcaa cacgttggga
      181 agcacttcgc ggaagagggt gcgggggcgc ctctggccga tggcgcgcga ggcctcgacg
      241 tactcccttt gagcctgatc gagggtctca ccgcgtgcca ggcggtagaa ctccacccac
      301 cccttcagcg agagcgcggc gacgaggttg gcgaaacccg gtccaaggaa ggccatcatg
      361 ccgatggcga agatcaggta agggaacgcg agcagtagct ccgtcacgcg cccgaccacc
      421 gcatcgaagg cgccgcgata gaagccgccc acggcgccga tcacggtccc gaaggcggct
      481 gtgatagcca cggtcagaac gccgaccagc agcgagacgc gcgccccgta gagggtcctt
      541 gtgaggatgt cgcggccgag cgaatcggtc ccgaaccagt gctgcgccga cgggggcgcg
      601 aggcgctgag ccagatcgcc agcctcgggt ccgtacggag ccaacacggg cgccagggcg
      661 gcggcgagca cgtacgtggc gactatggcc gcgctgatgt gccccagcgg cgtcgcgaac
      721 accctggctg cccggctcac agcgagatcc gggggttcag gagcgtgtag agggtgtcga
      781 cgacgaggtt cgctccgacg tacgtaaggg cgtagagcat cacggccgcc tgcacgacga
      841 cgtagtcgcg cgcgaagatg gagttgacga cgagcctccc gagccccggc caagcgaaga
      901 ccgtctcgac gatcatgttg ccgccgagca gctcaccgaa ctgtaggccc gtgacggtga
      961 gcgccggtat gagggcgttg cgtagggcgt gcttgaggat gacgccccat tcacgcaggc
     1021 ccttggcgcg cgcggtcgtc acgtagtcct taccgagtac ctcgagcatc gacgtgcgca
     1081 ctacgcgggc gagcaccgcc gcgaacacca ccccgagggt gagcgccggc aggaccaggt
     1141 gcttgagcgc ggtcccgaac gccttgaggt tgccagccag caacgtgtcc accagcatga
     1201 agccggtggt cctgtctacc accatggtcg ccgctatgcg gcccgacgtt ggcaggaccc
     1261 tcagctcgac cgcgaacagc aggatcatca gcagcccgaa ccagaacgcg ggcatgctga
     1321 tgccgaccag ggcgcccgac agcacggtgc ggtccagcca ggagcccgcc cgcacggcgc
     1381 tgatgatgcc tacgggcatg gcgatgagga tgccaacgag gacggtcgca agggtgagtt
     1441 ctatcgtggc aggaagggca tccccgatga gcttggccac cggcgcgttc ttgacgatgc
     1501 tcaccccgag gtcgccccgg aggaggcccg acaggaagat gccgagctgc gttaggagcg
     1561 gtttgtcgag gtggagttgg ctcctgaggg agtcgagctg ctgctgcgac acctgggtat
     1621 cgccgagcat gatctcgacc gggtcgcccg ggaggaagcg caggaacatg aacgcgagga
     1681 ccgctacccc aagggctacc gggatggcgg acagcacgcg ttctagcagc ttggtcaggc
     1741 gcatcgggtc ctcaatgcca atgggttgac gcggagcgcc gctagcgatc gaatccaggc
     1801 cctctcagct gaacaccagg gtggcgcccc gcgccagggg caggggcgcc accctgatca
     1861 cccggtgatc agttcgtctc gtagccggcc tgttggagaa gctccttggc cttggccggg
     1921 tcgtacgggt agggcgccag gtcctgagcg tagccgaagc cgctcgggag gaacaccgtc
     1981 gacagcctat cggcgtagcc ccggtagatg ttcttcagga tgctgtccca atcgatcgcg
     2041 tagttcagcg cctggcgcac gcgagtgtca ttgaacggcg cggcctcgtt gttcagctcg
     2101 atctggtagg agcgcgttcc gagcgcggtt cggacgctga cgcccggcac gctggcgatg
     2161 cgatcgacaa ggtcgggtgg cacggcctgg atcacgttga cctcgccagc gagaagcgcg
     2221 gcgatgcgcg ttgacgcttc cggcatcatc tgccatacga cgctcttcaa ctcgggcgcg
     2281 ccgttccaat gggtggggtt ggcctcgagc acgacctggg aatccagggc gccacgcgcg
     2341 tagacgaacg gcccggagcc gatcggcttg gcggtgaagg ccgctacgcc tacctcggtc
     2401 aggtagtcct tcggcacgat ctggaagtgg gtcagcgcct gtaggacgag ctcttgcggg
     2461 aaggtctcct tgtagacgaa gcggacggtc gtcgcgtcga ccttctcgac gtggtcgatg
     2521 gggccgagaa gaccggctcg cgggctcgtg gcgccgtcga tggcgccctc ggtgaggatg
     2581 cgctcgaagg tgaagacaac gtcgtccgcg tccaagacct cgccgttgct gaaggtcacg
     2641 ccggagcgca ggtcgaactc gtaggtcagt gggtcgacga ctcgccaagc cgttgccaga
     2701 tgcggttgca cctgaccttc ggaggtgggg atggtgaggg cgtcgaagat gttgcggagc
     2761 accgcctcgg tgtcgcggtc gcggtacatg gcggggtcga gcgtgacgac ggcatcgact
     2821 cgcatgccta tcacgagcgt gtcggagcct gcgaccgaga ctctggcgaa gttctcgctg
     2881 ttgtcggctg ccggttccca cccccgaacg ctgtccgagg ccgcttcgat gttctggagc
     2941 acgtaaccga acaccagggg cgcgtcagcg aagatctgtc tctgcgccgt gaggtacgcc
     3001 tcgttgcgag tcgcgtcgtc tatggaagtc gaggcgatgc ggaaggcctc gtccacggac
     3061 gggttcgagt agaaggagta gttcccgcgg tccttggtgc ggagcttggg gatcgccagg
     3121 tcgaacgggt cgaagtaggc gctcccccag tccgaggtgg acgccgagcg gttaccggcc
     3181 agcatctcgt ccctcaggac ggtcgtttgc cagacccgca acgacgcatc gatgcctatg
     3241 gcctgtagtt gggcgacgat cgcctggccc tcgtcggacc ggttctccgt gacgtccaag
     3301 acgaacgtca gtttctcggc gccggccgac atgaagacca gagcggccag cgatatgagc
     3361 aaccatttca tccgatgcat gccgtcctcc ttggaccacc ggcctgtcgc ggtggggtgc
     3421 tgctccgccc tc
//
LOCUS       DAOQYE010000102         3430 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_28805_length_3430_cov_2.973926, whole genome shotgun sequence.
ACCESSION   DAOQYE010000102 DAOQYE010000000
VERSION     DAOQYE010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 3430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3430
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28805_length_3430_cov_2.973926"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(122..1369)
                     /locus_tag="PKN52_02650"
     CDS             complement(122..1369)
                     /locus_tag="PKN52_02650"
                     /inference="COORDINATES: protein motif:HMM:NF017485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF815 domain-containing protein"
                     /protein_id="HNQ98870.1"
                     /translation="MSTDARTLFPGLLTASWWAPFEALSRYGTSAGERVTEMTFELAV
                     ALASAGHADPAAAVAANLLSERSALSEYACRPLPAGLASLLGLELERLGGAVRRVRAQ
                     SASDAGGGGAHAATPPLRDLAAPGLEPWAAANVDRLAGALSAADAPYLVATYLELLHA
                     VGNGPAALHPVARWTSGRLVPVAHPDLPDWSALVGLDGPLGALAGNTEALIAGGRTND
                     CLLYGARGSGKSTAVRGLVRRYVERGLRLVELQPEQLDSLPELVELLRFQPLKFVVFV
                     DDLSFEADDGRYRPLKSLLEGGVTQRPANTALYATSNRRHLVRERLRDRPDPLDDDVH
                     AWDTHHERLALADRFGLVITFPAADQRGYLEVVRALVAKLGAPSGGSASPSRSAEPSG
                     PAMPGWTWPSIMCSTGIRPTNGY"
     gene            complement(1369..2265)
                     /locus_tag="PKN52_02655"
     CDS             complement(1369..2265)
                     /locus_tag="PKN52_02655"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNQ98871.1"
                     /translation="MYRQPGEREEPQRIAIALGGGTARGMVHVGVLKALDDLGVRPTM
                     LGGTSFGAVIATLYALCGNAYELERVVRTQDIGEVWRQGLDFGLHRGAIVHGERLRDW
                     LDRKFFFGATFEDLPMPLAIATTDLATGELVVVASGSLADAVRASCALPGLFAPVRWR
                     DRWLIDGGFVEPVPFSTLTGVGVRKLGVHAGVDVRSSSVVRAIRRFNTTPFGRAFLAR
                     GERVTPRGPIGQIYRGLAISLGSYSRGLKVPPDARLLRVEPHISWWDFHKSPAAIDAG
                     ETAMRELIASGFLMAPTAVALP"
     gene            2332..>3430
                     /gene="dnaX"
                     /locus_tag="PKN52_02660"
     CDS             2332..>3430
                     /gene="dnaX"
                     /locus_tag="PKN52_02660"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HNQ98872.1"
                     /translation="MVDSEGEMSALYQRARPVTFDEVVGQDHVKDVLTAAIRRQRVGH
                     AYLFSGPRGVGKTTSARLLAMAVNCEADASERPCGVCESCRLVQAGSHPDVIEMDAAS
                     NNSVDDVRELRERTMLSSIRGGSRVWILDEAHMLSKAAANALLKTLEEPPPSLLFVLA
                     TTEPERLPPTVLSRCQHFRFRRLTDAEIVGKLARLTAGAGVAAETPALELVARSADGG
                     MRDAESLLDRLLAGGEAITLQRAEDALGLPPHERLRAMATALTGGDLTGLLDQAAALY
                     RAGFAPRTLAEQLARALRDALHARLAGTPWLEVGEDDLLRLLHALDDEQERFVRHDDL
                     YALEVALIKALNALRGNVPANFSMQSIASGEP"
BASE COUNT          553 a         1220 c         1184 g          473 t
ORIGIN      
        1 tcaccgggca taaaagattg gatgatatga taggattgta caccgtctgt cttgtggtgc
       61 ttttcctttg tccgcatcat gcgctgatag gcaaaatcgg gactgacaag ctgataagcg
      121 gtcaatatcc gttcgtcggt cttatccccg ttgagcacat aattgatggc cacgtccagc
      181 cgggcatggc ggggccgctg ggttccgcgg agcgactcgg cgacgccgac ccgcccgagg
      241 gggcgccgag cttcgccacc agcgcgcgta cgacctcgag gtagccgcgt tggtcggccg
      301 ccgggaaggt gatcaccagc ccgaagcggt ccgcgagcgc gaggcgttcg tggtgggtgt
      361 cccaagcgtg cacgtcgtcg tccagggggt cgggccggtc gcgcagccgc tcgcgcacca
      421 ggtggcgacg gttcgaggtg gcgtacaacg ccgtgttggc cgggcgctgc gtcacgcctc
      481 cctccaggag gctcttgagg ggccgataac gcccgtcgtc cgcctcgaag gagagatcat
      541 cgacgaagac cacgaacttc aacggttgga acctcagcag ctccacgagc tccggcaggc
      601 tgtccaactg ctcgggctgc agttcgacga ggcggaggcc gcgctcgacg tagcgcctca
      661 ccagcccgcg caccgctgtc gacttgccgc tcccccgcgc gccgtagagg aggcaatcgt
      721 tggttcggcc acccgcgatg agcgcctcgg tgttgccggc cagggcgccc aacggtccat
      781 cgagccccac cagcgccgac cagtctggca gatcggggtg cgcaacgggc acgaggcgcc
      841 ccgaggtcca ccgcgcgacc ggatggaggg ccgccgggcc gttccctacc gcatgcaaca
      901 gctccagata ggtggccacc aggtagggtg cgtcggccgc agaaagcgcg ccggcgagcc
      961 tatcgacgtt ggcggccgcc caaggctcga gccccggcgc ggcgaggtcg cgcaagggcg
     1021 gcgtggcagc gtgagccccg ccgccgccgg cgtcggacgc cgactgggca cggacccgcc
     1081 tcaccgcgcc tcccaggcgt tcgagctcga gccccagcag ggacgccaga ccggcgggaa
     1141 gcggcctgca cgcgtattcg gagagcgcag agcgttccga gaggaggttg gctgcgaccg
     1201 cggcggccgg gtcggcatga ccagcgcttg ccaaagccac ggccaactcg aaggtcatct
     1261 cggtgacccg ctcgcccgcc gaggtcccat agcggctcag cgcctcgaaa ggcgcccacc
     1321 atgacgccgt gaggaggccg gggaacaggg tacgcgcgtc cgtcgacatc agggcaaggc
     1381 taccgctgtc ggcgccatga ggaaaccgct cgcgatcagt tcccgcatgg ccgtctcgcc
     1441 cgcgtcgatg gcagcggggg acttatggaa gtcccaccag gagatgtggg gctcgacgcg
     1501 taagagccgg gcgtcaggcg gcaccttgag cccacggctg tatgagccga gggagatggc
     1561 gagcccgcgg tagatctgac ctatcggccc gcggggcgtc acacgctccc cgcgcgccag
     1621 gaaggcgcgg ccgaacggtg tggtgttgaa ccgacggatg gccctcacga ccgacgagct
     1681 acgcacgtcg acgccggcgt gcacccccag cttgcgcacc ccgacgccgg tcagggtcga
     1741 gaagggcacc ggctcgacga agccaccgtc tatcaaccac ctgtcgcgcc accgcacggg
     1801 ggcgaacagg ccgggcaggg cgcagctcgc gcgcaccgcg tcggccaacg acccgctggc
     1861 cacgaccacg agctcgccgg tggccaggtc ggtcgtcgcg atcgccaacg gcatgggcag
     1921 gtcctcgaac gtggccccga agaagaactt cctgtcaagc cagtcgcgca gccgctcgcc
     1981 gtgcacgatg gcgccgcggt ggagaccgaa gtcgaggcct tggcgccaga cctcgcctat
     2041 gtcctgggtg cgcacgacgc gctcgagctc gtaggcgttg ccgcatagag cgtaaagggt
     2101 cgcgatgacc gcgccgaacg acgtgccacc gagcatcgtc ggccttacgc ctaggtcgtc
     2161 cagggccttg agcacgccga cgtgcaccat ccctcgcgcc gtgccacccc cgagggcgat
     2221 ggcgatcctc tgtggctctt cgcgctcccc cggctgccgg tacatgtgcg aatgctacta
     2281 ccgcgaccac cgcgcggccg aagcgcccgg aaccggtcga cgcgcgggtc gatggtagat
     2341 tctgaaggcg agatgtcggc cctctaccaa cgcgcgcggc cagtcacgtt cgacgaggtc
     2401 gtcgggcagg accacgtcaa ggacgtgctc accgccgcga tccgccgcca gcgtgtcggc
     2461 cacgcgtacc tcttctcagg cccccgcggc gtcgggaaga cgacgagcgc gcgcctgctc
     2521 gcgatggccg tcaactgcga ggccgacgcg agcgagcgcc cctgcggcgt ctgcgagtcg
     2581 tgccggctcg tccaggccgg cagccatccg gacgtcatcg agatggacgc cgcctcgaac
     2641 aactccgtcg acgacgtgcg cgagctgcgt gagcggacca tgctctccag catcaggggc
     2701 ggcagcaggg tgtggatcct cgacgaggcg cacatgctga gcaaggcagc cgcgaacgcg
     2761 ctgctgaaga ccctcgagga gccgccgccc agcctgctgt tcgtgctcgc tacgaccgag
     2821 ccagaacgct tgcccccgac cgtgctgtcg cgctgccaac acttccggtt caggcggctg
     2881 acggacgcgg agatcgtcgg caagctagcg cggctcacgg ccggcgccgg ggtggcggcg
     2941 gagacgcccg cgctcgagct cgtggcgcga agcgccgacg gcggcatgcg cgacgccgag
     3001 tcgctcctcg accggctgtt ggccggcggc gaggccatca cgctgcagcg cgcggaagac
     3061 gcccttggcc tgcccccgca cgagcgactg cgagccatgg ccaccgcgct cacgggcggc
     3121 gacctgactg ggctcctcga ccaggctgcc gccctctacc gcgccggctt cgctccgcgc
     3181 accctcgccg agcagttggc gcgcgctctc cgagacgcgc tccacgcccg cctcgccggc
     3241 acaccgtggc tggaggtggg cgaggacgat ctcctgcgcc tcctgcacgc gctagacgac
     3301 gagcaggagc ggttcgtgcg ccacgacgac ctctacgcac tcgaggtcgc tctcatcaag
     3361 gcgctcaacg cgttgcgcgg caacgtcccg gccaacttct ccatgcagtc catcgcgtcc
     3421 ggggagcctg
//
LOCUS       DAOQYE010000103         3414 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_29017_length_3414_cov_3.988687, whole genome shotgun sequence.
ACCESSION   DAOQYE010000103 DAOQYE010000000
VERSION     DAOQYE010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 3414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3414
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29017_length_3414_cov_3.988687"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="PKN52_02665"
     CDS             complement(<1..229)
                     /locus_tag="PKN52_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98873.1"
                     /translation="MAEHTTAKAIEAPHVHATVSDLAAARRIGILLDVVAMGGAAGDV
                     LDVYVDVLGPDGATWLNAVHFSQIAGNGAAAK"
     gene            complement(262..438)
                     /locus_tag="PKN52_02670"
     CDS             complement(262..438)
                     /locus_tag="PKN52_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98874.1"
                     /translation="MPYVVTRGEVIDGGRRYKCGEFVPGVKGELADMEAVGAVKWVEQ
                     KPREPCARRTKAVK"
     gene            complement(443..1090)
                     /locus_tag="PKN52_02675"
     CDS             complement(443..1090)
                     /locus_tag="PKN52_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98875.1"
                     /translation="MGKIYRIPAQKVEVGLLECRNGFGTLKTTDTGADFPVFACTEVF
                     ENPKATASTGCLGATALPSGAATTVTEGITQPDYPRSLQMVASAAATSVVTAYGYDQF
                     GNAIDEAFTLNGTSDVPGTKVFASITKFVLATRIDGAADLTVGFGKILGTSRRVLGLA
                     LDGAVFATSGGKLASVQETTRPVKATTAGVQGAYFNTALAATNTYILRYHTDEIR"
     gene            complement(1157..2101)
                     /locus_tag="PKN52_02680"
     CDS             complement(1157..2101)
                     /locus_tag="PKN52_02680"
                     /inference="COORDINATES: protein motif:HMM:NF015802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HNQ98876.1"
                     /translation="MAIVNGHINAALSQFAAGTATNAQARMIAQLVAPEITVDKQSDS
                     YYIFDAADLRIDKPEYSAGANVARVVRSESEGTYKCKEFALEEAIPWGKLANADAGLR
                     IERRATAGLVNKLMLRREKAVADVLFATGTFTNTAALAGANRWDVDTSNPVKAVSDAR
                     EAVRLLCGLDPNTVVMGAAVWAALRNHPDITSRVAGLVAGTPATEAQAAAALGIERVL
                     VGTAVYNAAAEGAAASNANVWGKFASVCYIDPAAGRDVDGAIVPMQQFVWDGVADPFS
                     TFTYESNESRSHVVQCYDAVDAKAISINSAYLYSTVVS"
     gene            complement(2117..2497)
                     /locus_tag="PKN52_02685"
     CDS             complement(2117..2497)
                     /locus_tag="PKN52_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98877.1"
                     /translation="MSQATSANSVIKTFVAGGTIHPGHAVTVSGTSVVEASGVNAANG
                     IYVGEADCGSGDHVPICIAGPCRAWCDATSAITLFANLATDANGHLVVDTTDKHKLVG
                     QALEPLASGEGFVEVNVNIGWLAA"
     gene            complement(2502..>3414)
                     /locus_tag="PKN52_02690"
     CDS             complement(2502..>3414)
                     /locus_tag="PKN52_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98878.1"
                     /translation="VRTYLRETLAKAGAVKLAEPGSVEERRRLLQEAVAAAFPQPGGE
                     EPLIEDFGPDWLIWSTWSGGEPRYWRCPYEATDQGITLGQPVEVKRETTYVPVSDGAP
                     GAALPSDSQAIAMGEAGEGAGAATVEDHAARKGVERRMNEKALSILQLAEDASEEAQS
                     AAVLALAEERDDLQRQLDERDKAERTREFEAKLAEAMKPDEQQLVHILPGEREVYLAL
                     AEVDHARALAAIEARMQGPGLKLGAVGSPAEGDSDQERPADVVLAEMAALLSAKDGID
                     IVTAQDRVLAEHPELKTRLFAAEQEVR"
BASE COUNT          436 a         1173 c         1216 g          589 t
ORIGIN      
        1 gcttggccgc ggcgccgttg ccggcgatct gcgagaagtg gaccgcgttc agccaggtcg
       61 cgccgtcggg gccgagcacg tcgacgtaca cgtcgagcac gtcgccggcg gcgccgccca
      121 tcgcgaccac gtcgagcagg atgccgatgc gccgcgcggc ggcgagatcg ctgaccgtgg
      181 catgcacgtg cggggcctcg atggctttcg cggtcgtgtg ctcggccacg gccgccaggg
      241 tgagaacgct cggatagtgc atcacttcac cgccttggtc cgtcgggcac acggctcacg
      301 cggcttctgc tcgacccact tgaccgcgcc gaccgcctcc atgtcggcca gctcgccttt
      361 gacgcccggc acgaactcgc cgcacttgta gcgtcgcccg ccgtcgatga cctcgcctcg
      421 ggtgactacg tacggcatgg cgtcacctga tctcgtcggt gtggtagcgc aggatgtagg
      481 tgttggtggc ggccagggcg gtgttgaagt aggcgccctg cacgcccgcc gtggtcgcct
      541 tgaccggccg ggtggtctcc tgcactgagg ccagcttgcc gccgctggtc gcgaacacgg
      601 ccccgtcgag cgccaggccg agcacgcgcc ggctggtgcc gaggatcttg ccgaagccga
      661 ccgtgaggtc ggcggccccg tcgatgcgcg tggcgagcac gaacttggtg atcgaggcga
      721 acaccttggt gccgggcacg tcgctggtcc cgttcagagt gaacgcctcg tcgatcgcgt
      781 tgccgaactg gtcgtatccg tacgcggtca ccacgctggt ggccgccgcc gaggcgacca
      841 tctgcagact gcgtgggtaa tccggctggg tgatgccttc tgtgaccgtg gtagcggccc
      901 cgctcggcag cgcggtcgcg cccaggcagc cggtggaggc ggtcgctttg ggattctcga
      961 agacctccgt gcaggcgaag accgggaagt cggcgccggt gtcggtcgtc ttcagcgtac
     1021 cgaagccgtt gcggcactcc agcaggccga cctcgacctt ctgggccggg atgcggtaga
     1081 tctttcccat ctctgtcctt tcattggccg ccggggccgg gatcggccga ccccggcggc
     1141 atgacccgga tcagactcag ctgacgacgg tggagtacag gtaggcgctg ttgatgctga
     1201 tcgccttggc gtccacagcg tcgtagcact gcacgacgtg cgaacgggac tcgttggact
     1261 cgtaggtgaa ggtgctgaac gggtccgcca cgccgtccca gacgaactgc tgcatcggca
     1321 cgatggcgcc gtccacgtcg cggccggcgg cggggtcgat gtagcagacc gaggcgaact
     1381 tgccccagac gttggcgttg ctggccgcgg cgccctcggc ggcggcgttg tagaccgccg
     1441 tcccgaccag gacgcgctcg atgccaagcg cggccgcggc ctgcgcctcg gtggccggcg
     1501 tcccggcgac caggccggcg acgcgcgagg tgatgtcggg gtggttgcgc agtgcggccc
     1561 agacggcggc gcccatgacg accgtgttcg ggtcgaggcc gcagagcagc cgcaccgcct
     1621 cacgtgcgtc ggagaccgcc ttgaccgggt tggacgtgtc cacgtcccag cggttggccc
     1681 cggccagcgc ggccgtgttg gtgaacgtgc cggtcgcgaa gagcacgtcg gcgaccgcct
     1741 tctcacgccg cagcatgagc ttgttcacga gcccggcggt ggcgcgacgc tcgatgcgca
     1801 ggccggcgtc ggcattggcg agcttgcccc aggggatggc ctcctccagg gcgaactcct
     1861 tgcacttgta ggtgccctcg ctctcgctgc gcacgacgcg ggccacgttg gccccggccg
     1921 agtactccgg cttgtcgatg cgcaggtcgg cggcgtcgaa gatgtagtag ctgtccgact
     1981 gcttgtcgac cgtgatctcc ggcgcgacca gctgggcgat catgcgcgcc tgcgcgttgg
     2041 tggccgtccc ggctgcgaac tgcgagaggg cggcgttgat gtgtccgttg acgattgcca
     2101 cgatgcctcc ctcctatcag gcggcgagcc agccgatgtt gacgttgacc tcgacgaaac
     2161 cctcgccgct ggcgagcggc tcgagggcct ggccgaccag cttgtgcttg tccgtcgtgt
     2221 cgaccacgag atgcccgttt gcgtcggtcg ccaggttggc gaacagggtg atggcgctgg
     2281 tggcgtcgca ccaggcgcgg cacgggccgg cgatgcagat cggcacgtgg tcgccgctgc
     2341 cgcagtcggc ctcgccgacg tagatgccgt tggcggcgtt gacgccgctg gcttcgacca
     2401 cactcgtgcc cgagacggtc acggcgtggc cgggatggat cgttccaccg gccacgaacg
     2461 tcttgatgac gctgttggca ctggttgcct ggctcatgtt ctcacctcac ttcttgctct
     2521 gcggcgaaca ggcgggtctt gagctcggga tgctcggcca gcacccggtc ctgcgccgtg
     2581 acgatgtcga tcccgtcctt ggccgacagc agggctgcca tctcggccag caccacgtcg
     2641 gcgggccgct cctggtcgga gtcgccctcg gcgggcgagc ccactgcgcc gagcttcaga
     2701 ccggggccct gcatgcgagc ctcgatcgca gccagcgctc gtgcgtggtc gacctctgcc
     2761 agggcgaggt agacctcgcg ctcgccgggc aggatgtgga ccagctgctg ctcgtcgggc
     2821 ttcatcgcct cggcgagctt ggcctcgaac tcccgcgtgc gctcggcctt gtcgcgctcg
     2881 tcgagctgcc tctgcaggtc gtcgcgctcc tcggccagag cgagcacggc ggcgctctgc
     2941 gcctcctcgc tcgcgtcctc ggccagctgg aggatgctca gggctttctc gttcatgcgt
     3001 cgttcaaccc ctttccttgc ggcgtgatcc tcgacggtcg cagcacccgc accttcgccc
     3061 gcctcgccca tggcgatggc ctgcgaatcg gaggggagtg cggcgccggg agcgccgtcg
     3121 ctgacgggga cgtaggtcgt ctcccgcttg acctcgaccg gctggccgag ggtaatgcct
     3181 tggtccgtcg cctcgtacgg gcagcgccag tagcggggct caccgcccga ccaggtgctc
     3241 cagatgagcc agtcggggcc gaagtcctcg atcaggggct cctcgccgcc gggctgcggg
     3301 aaggccgccg cgacggcctc ctggagcagc cggcggcgct cctcgaccga gcccggctcg
     3361 gccagcttga ccgcgccggc cttggccagc gtctcgcgca ggtaggtgcg cacg
//
LOCUS       DAOQYE010000104         3387 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_29374_length_3387_cov_3.634754, whole genome shotgun sequence.
ACCESSION   DAOQYE010000104 DAOQYE010000000
VERSION     DAOQYE010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 3387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3387
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29374_length_3387_cov_3.634754"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(75..884)
                     /locus_tag="PKN52_02695"
     CDS             complement(75..884)
                     /locus_tag="PKN52_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNQ98879.1"
                     /translation="MVFHDVQGARVPALGFGTYLLKGDDCVEGVRHALELGYRHIDTA
                     QSYENEAEVGLGLRLGGVPRSEVFLVTKLRPSNFARAADATHASLAALGTDYVDLMLL
                     HWPKDEASVAAALDALARLQADGAVKHIGVSNFPTQLVRAASGVTRLFCNQVEYHPFL
                     AQARVLAQAEELDMLVTAYRPLAKGMLQGEPVLRELGAKYGKTPEQVTLRWLMQQPRV
                     ATIPKSADPRRRAQNLDVFDFELAPTEMAAVAGLARGHRLVNPENAPEWDA"
     gene            complement(989..2896)
                     /locus_tag="PKN52_02700"
     CDS             complement(989..2896)
                     /locus_tag="PKN52_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HNQ98880.1"
                     /translation="MLGSFRSPRAVVKLAIDVGLFLIATPLAFALRYDGNLGSEHPLL
                     LAMVVLVPVKAGLDYLLGLPNRSWRGLTFRDLSSLMLHAGGVLAFGLILIALFWQQLG
                     VGPSLALLDAGITFMLMAGVRAFARYRNEHMTASIIGPDYRKRVLVVGAGEAGALVVR
                     ELHKHPETGMLPVAFLDDDPRKRGQRVAGVPVVGTVDTAAEVIVSNGIDQVLIAMPSK
                     GGRAIRKVIEQVREARPNLVYKIIPGMYEVLSGQVDVRRIREVDIDDLLGREPIKLDT
                     EAILDYLGGKRVMITGAGGSIGSELVRQICRFNPAELILFGHGENSIYTLERELDRDW
                     PHIRYHGVIGAIQNGARLDYVFTRYRPDVVFHAAAHKHVPLMEENPEEAVFNNVIGSR
                     NLINLALKHGVTHFVNISTDKAVNPTSVMGASKRMVEFLVQDAARRAGPGQTFVSVRF
                     GNVLGSRGSVIPIFKSQIAAGGPVTITHPEMVRYFMTIPEAAQLVLQASGNGRNGEVY
                     ILDMGEPVRIVDLARDLIRLSGFEPDVDIPIRFTGMRPGERLVEELMTEKERTSQTAH
                     EKIFVARTEPVDREGLTSTIEGLQDAAMRSDHARIRVLFEGFLDGCHFAPVVPVKDSS
                     GDLPGSQRLPS"
     gene            complement(2896..>3387)
                     /locus_tag="PKN52_02705"
     CDS             complement(2896..>3387)
                     /locus_tag="PKN52_02705"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HNQ98881.1"
                     /translation="QKRVGLSGVPFEILKFRTMRVDAEKVGAQLTVGADPRITKVGAF
                     LRAWKIDELPQLVNVVKGDMALVGPRPEVPRYVELYTPEQRRVLSVRPGITDPASIEF
                     RNESELMAGQDDPERYYREVIMPRKVQLNLDYLDRRSLATDLGILFATAKAVLLGREG
                     EEA"
BASE COUNT          561 a         1075 c         1157 g          594 t
ORIGIN      
        1 gtgcgcgccg cgctccagca gggcccgcac catgtgcgag ccgatgaagc cggccccgcc
       61 cgtcaccagc accctcatgc gtcccactcc ggcgcgttct cggggttcac gaggcggtgg
      121 ccgcgcgcga ggccggccac ggccgccatc tcggtcggcg cgagctcgaa gtcgaagacg
      181 tccaggttct gggcgcgccg ccgcgggtcg gccgacttcg ggatggtcgc gacgcgcggc
      241 tgctgcatga gccacctgag cgtcacctgc tccggcgtct tgccgtactt cgcgcccagt
      301 tcgcggagca cgggctcgcc ttgcagcatg cccttggcga gcggacggta ggccgtcacc
      361 agcatgtcca gctcctcggc ctgggccagt acgcgcgcct gggcgaggaa cgggtggtac
      421 tcgacctggt tgcaaaagag cctcgtcacg cccgaggcgg cgcgcacgag ctgggtgggg
      481 aagttgctga ccccgatgtg cttgacggca ccgtccgcct gcaggcgcgc cagcgcatcc
      541 agtgccgccg ccacggacgc ctcgtcctta ggccagtgca ggagcatgag gtcgacgtag
      601 tcggtgccga gcgcggcgag gctggcgtgg gtagcgtccg ccgcgcgcgc gaagttggag
      661 gggcgcagct tggttaccag gaagacctcg gagcggggca cgccgccgag gcgcaggccc
      721 aggccgacct cggcctcgtt ctcgtagctc tgggccgtgt cgatgtgccg gtagccgagt
      781 tcgagggcgt ggcgcactcc ctcgacgcag tcgtcgccct tcagcaggta ggtgccgaac
      841 cccagcgctg gcacgcgcgc tccctggacg tcgtggaaga ccatatctgg atgctaactg
      901 ctcgacgagg cggggaaacg tggttttcct gacgtaggcc cgagaccgtc aggttcccaa
      961 gagcagtgac ggtcggcgaa gaccgaactc agctcggtag gcgctggcta ccaggtaggt
     1021 cgccgctact gtccttgacg ggtaccacag gggcgaagtg gcacccgtcg aggaagcctt
     1081 cgaagagaac ccggatgcga gcatggtccg agcgcatggc ggcgtcctgc aagccctcta
     1141 tagtcgatgt caggccctcg cggtccacag gctccgttcg cgccacgaat atcttctcgt
     1201 gcgccgtctg ggacgtgcgc tccttctccg tcatcaactc ctcgacgagc cgttcgccgg
     1261 gacgcatgcc cgtgaagcgg attgggatgt cgacgtcggg ttcgaagcca gacaggcgga
     1321 tgaggtcgcg ggcgaggtcg acgatgcgca caggctcgcc catgtcgagg atgtacacct
     1381 cgccgttgcg gccgttgcca gaagcctgca acacgagctg cgcggcctcc gggatggtca
     1441 tgaagtaccg caccatctcc gggtgagtga tggtcacggg tccgcctgcc gcgatctgcg
     1501 acttgaagat cgggatgacg ctgccgcggc tgccgagcac gttgccgaag cgcacggaga
     1561 cgaacgtctg cccgggaccg gcgcgcctgg cggcgtcctg cacgaggaac tcgaccatgc
     1621 gcttgctggc gcccatgacg gaggtcgggt tcactgcttt gtcggtcgag atgttcacga
     1681 agtgggtgac gccgtgctta agggcgaggt ttatgaggtt gcggctgccg atgacgttgt
     1741 tgaagacggc ttcctcgggg ttctcctcca tgaggggaac gtgcttgtgg gcggccgcgt
     1801 ggaacacgac atctggacgg tagcgggtga atacgtagtc gaggcgagcg ccgttctgga
     1861 tggcgccgat gacaccatgg taccgaatgt gcggccagtc gcggtccagc tcgcgctcga
     1921 gcgtgtagat gctgttctcg ccgtgtccga agaggatgag ctcggccggg ttgaaccggc
     1981 agatctggcg cacgagctcg gagccgatcg agccgcccgc ccccgtgatc atcacgcgct
     2041 tgccacccag gtagtcgagg atggcctccg tgtcgagctt gatgggctca cgaccgagga
     2101 ggtcgtcgat gtcgacctcc cggatgcgcc gcacgtcgac ctgcccggac aggacctcgt
     2161 acatgcccgg gatgatcttg tagacgaggt tgggtcgcgc ctcgcgcacc tgctcgatga
     2221 ccttgcggat agctcggccg cccttagatg gcatggcgat gaggacctgg tctatgccgt
     2281 tcgagactat gacctcggcc gccgtgtcta ctgtgccgac tacaggtacg cccgccacac
     2341 gctggccccg cttgcgtgga tcgtcatcga ggaacgctac aggcagcatg ccggtctcgg
     2401 gatgcttatg gagttcgcgc acgaccagcg cgcctgcctc gcctgcccct actactagca
     2461 cacgcttgcg gtagtcgggg ccgatgatgc tggccgtcat gtgctcattc cggtagcgtg
     2521 cgaaggcgcg taccccggcc attagcatga aggttatgcc cgcgtcgagt aacgcgagcg
     2581 agggaccgac tcctaactgc tgccagaaaa gagcgatgag gatgagcccg aaggcgagaa
     2641 cacctccggc gtggagcatg aggctgctga ggtcccgaaa ggtcaaaccc cgccatgacc
     2701 tgttcgggag tccgagcaag tagtctaggc cggccttaac aggaaccagc acgaccatcg
     2761 ccaggaggag cgggtgctct gacccaaggt tgccgtcgta gcgcaaggcg aacgcaaggg
     2821 gtgtggcgat aaggaacagg ccgacgtcta tggccagctt gactacggcc cttggggacc
     2881 ggaacgagcc gagcatcacg cttcctcacc ttccctgccg aggagtaccg ccttcgcagt
     2941 tgcgaagagt atcccaagat cggtggctag gctgcgcctg tcgaggtagt ccaggttgag
     3001 ctgaaccttg cggggcatga tcacctcgcg gtagtagcgt tcagggtcgt cctgcccggc
     3061 catcagctcg ctttcgttgc ggaactcgat ggaggccggg tcggtgatcc cgggacggac
     3121 gctcagaacc cggcgctgtt ccggcgtgta caactcgacg tagcgcggaa cctctgggcg
     3181 tgggccaacg agcgccatgt cgccctttac gacgttgacg agttggggga gttcgtctat
     3241 cttccacgcc cgcaggaagg cgcccacctt cgtgatgcgc gggtcggccc cgacggtcag
     3301 ctgcgcccct accttctcgg cgtccactcg catggtgcgg aactttagga tctcgaaagg
     3361 caccccgctc agccccaccc gtttctg
//
LOCUS       DAOQYE010000105         3310 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_30377_length_3310_cov_1.995084, whole genome shotgun sequence.
ACCESSION   DAOQYE010000105 DAOQYE010000000
VERSION     DAOQYE010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 3310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3310
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30377_length_3310_cov_1.995084"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(16..1137)
                     /locus_tag="PKN52_02710"
     CDS             complement(16..1137)
                     /locus_tag="PKN52_02710"
                     /inference="COORDINATES: protein motif:HMM:NF024770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNQ98882.1"
                     /translation="MSDQQRGRTRPRLVDVAKRAGVSIAVASTILNNRAASTIRASAE
                     TAERVRQAARELGYSPNPVAQRLARGRNQILGVFTFEPIFPLAQKSFYHPFLIGIEQE
                     AEDLSYDLLLFTSARSDPGRRQRQIFRGGVNRLALADGSILLGREEDRSEVSRLIEQR
                     YPFVYLGRRAITGFDVPYVGADYVSATAQVVRRLVGLGHRSFLYLGSLGDHEASLDRR
                     AGYAHALEELGLPNDPARQRRIQPGEISGDRLEAWLAAGVTAFVIEDDMVATRLLEVA
                     ASRGVRVPDAMSVALLGDALEHVEADVPWTMFRIPREAMGRQAVKLLAAILAARTRAP
                     GADAAVEEDAPAASRQIVLSCSLVEGATAGRAPAPSGVD"
     gene            complement(1142..2614)
                     /locus_tag="PKN52_02715"
     CDS             complement(1142..2614)
                     /locus_tag="PKN52_02715"
                     /inference="COORDINATES: protein motif:HMM:NF024941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4127 family protein"
                     /protein_id="HNQ98883.1"
                     /translation="MALLPLDDRPYNLSYVRGLARLVGRELHVPPPGLIGTFFTPGQP
                     EACGRWLLDLPEGVSTVIVALDMLTCGGLLASRSEAIDRDGALERLSSLERLREGRPR
                     IKVLAFDTIIRTSLSARDPESRRVHRLLHRFTTLEGKLRHAPDESTRREYDHVAGSIP
                     ASVVRRYRAVRERKHAVNRRAVELVAAGAIDVLHLLMEDVGPEGDKFGVHKEEQQALL
                     AMVGEHGIADRVFLQNGTDEGACTLLARAVTAERGAAPVFAVRYSAPDGADQVPTFED
                     RPFGANLASFVAAVGGKLTRDDREADAVLAVHTPYGQAPDLHGFVADIAGLISRGVPV
                     ALVDTTTNQADLALMDALGKEIELDRLSAYSAWNTACNSLGTALAQIVLVLAAPNDPG
                     LGKMNRALLLERLLDDYIYEGIVRPKVNEELEREGANPWDLLERAEEVRARVAVELAA
                     EARRTLQPRVGTRFAFTASLPWPRTFEPVIEARVLGGDLG"
     gene            complement(2617..>3310)
                     /locus_tag="PKN52_02720"
     CDS             complement(2617..>3310)
                     /locus_tag="PKN52_02720"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNQ98884.1"
                     /translation="GGSKRVQVDVRVVSATSKDGTVVTAEYDRLMLATGSNPFILPIP
                     GKDFAFLVVLGTMMIPSHVTTIPLFILARNFPLVGGNDVMGAGGTGLLNTYLGIALPH
                     LVLTFTIFLARQFFLDLPAEIRDSGRIDGASEVRVFASLYLPISLPIVATIAIFSFVT
                     AWDDFFWPLVVTTTDRMRTAQIGLSVFRSQFSSEWGALFAASVLIVLPVVIVFLFNQR
                     LFIRGLATGATK"
BASE COUNT          560 a         1100 c         1124 g          526 t
ORIGIN      
        1 cgctgctacg cattgtcagt caactccgga tggcgccggg gcccgcccgg cggtagcgcc
       61 ctcgaccagc gagcagctca ggacgatctg tctgctagcc gcgggggcat cttcttcgac
      121 cgcggcatcg gccccgggcg ctcgggtcct cgccgccagg atggcggcaa gcaacttgac
      181 ggcctgcctg cccatggcct cgcgcgggat gcggaacatg gtccaaggaa cgtcggcctc
      241 gacgtgctcg agcgcatccc cgagaagagc gaccgacatg gcatcgggta cgcgaacgcc
      301 cctggaagcc gcgacctcca gtaaccgcgt cgccaccatg tcgtcctcta ttacgaacgc
      361 ggttacgcca gcggccagcc aggcctcgag ccggtcgccg ctgatctcgc ccggttggat
      421 ccggcgctgc cgtgcggggt cgttcggtag gcccaactcc tccagcgcat gcgcgtaacc
      481 cgcacgccgg tcgaggctcg cctcatgatc gccgagcgag ccgaggtaga ggaagcttct
      541 gtgaccgagc cctaccaacc ggcgcacgac ctgggccgtg gccgagacgt agtcggcacc
      601 aacgtaaggg acgtcgaagc cggtgatggc gcggcgaccc aggtatacga atgggtagcg
      661 ctgctcgatg agccggctca cttccgaccg gtcctcttcg cgtcccaaca ggatggagcc
      721 gtccgccagc gccagacggt tgacgccccc cctgaagatc tgccgctgcc gccgccccgg
      781 gtcgcttctc gcactagtga agagcaggag gtcgtagctc aggtcctccg cctcctgctc
      841 gatgccgatc aagaacgggt ggtagaagct cttctgcgcc agcgggaaga tgggctcgaa
      901 cgtgaacacg cccaggatct ggttgcgtcc cctcgcgagc ctctgcgcca cggggttggg
      961 cgagtacccg agttcgcgcg ccgcctggcg cacgcgttcc gcggtctccg cgctggcgcg
     1021 gatggtgctt gcggcgcggt tgttgaggat ggtcgaggcg acggcgatcg agacgccggc
     1081 gcgcttggcg acgtcgacta gccggggccg cgttcggccc cgctgctggt cactcatccg
     1141 atcacccgag atcgccgccg agcacccttg cctcgatgac cggctcgaag gtacggggcc
     1201 aagggaggct ggccgtgaac gcgaaccggg tgccgacacg cggctgcagg gtgcgccgcg
     1261 cctccgccgc caactcgacc gctacccgcg ccctgacttc ctccgccctc tccagcaggt
     1321 cccatgggtt ggccccctct cgctcgagtt cctcgttgac cttgggcctg acgatacctt
     1381 cgtaaatgta gtcgtcgagg aggcgctcta gcagaagtgc gcggttcatc ttgccgagcc
     1441 cagggtcgtt gggtgccgcc agtaccaata cgatctgggc caaggccgtc cccaacgaat
     1501 tgcaggccgt gttccaggcg gagtacgcgc tgaggcggtc gagctcgatc tccttgccca
     1561 aggcgtccat cagtgccagg tcggcctggt tggtggtcgt gtcgaccaac gctaccggca
     1621 cgccgcgtga gatgaggccc gcgatatccg cgacgaaccc atgcaggtcg ggagcttgcc
     1681 cgtaaggcgt atggacggcc aacacggcgt ccgcctcgcg gtcgtcgcgg gtgagcttac
     1741 cgcccaccgc tgccacgaag ctggccaggt tagcgccgaa cggccggtcc tcgaacgtag
     1801 ggacctgatc cgcgccgtcc ggggcggagt accgaaccgc gaagacgggc gccgcgccgc
     1861 gctccgccgt aaccgcccgc gccaacaacg tgcatgcccc ctcgtcggta ccgttctgca
     1921 ggaacacgcg gtccgctatg ccgtgctcgc ctaccatggc gagcagggcc tgctgctctt
     1981 ccttgtgcac tccgaacttg tcgccctcgg ggccgacgtc ctccatgagg aggtgcagaa
     2041 cgtcgatggc tccggcggcc accaactcga cggcgcgccg gttgaccgca tgcttgcgtt
     2101 cgcgcacggc gcggtaacgg cgaacgacgc ttgcggggat gctgccggcc acgtggtcgt
     2161 actcacgccg cgtggactcg tcgggggcgt ggcgaagttt gccctccagg gtggtgaagc
     2221 ggtgcagtag gcgatggacg cgccttgact ccgggtctct ggcagacagc gacgtcctga
     2281 tgatggtgtc gaaggccagc accttgatcc tgggacgccc ctcccgcagc cgttcgaggc
     2341 tggacaagcg ctccaaggca ccgtcccggt ctatcgcctc ggaacgtgag gcgaggagcc
     2401 ccccgcacgt gagcatgtcg agcgccacga tgacggtgct tacgccctcg ggcaggtcga
     2461 gcagccagcg tccgcatgcc tccggctgcc cgggggtgaa gaacgtgccg atgagccccg
     2521 gagggggcac gtgcagctcg cgacctacga gccgcgccag gcccctcacg taagagaggt
     2581 tgtagggccg gtcgtcgagc gggagcaacg ccatgctcac ttcgtcgccc ctgtcgccaa
     2641 gccacgaatg aagaggcgtt ggttgaagag gaagacgatg acgacgggca ggacgatcag
     2701 cacggaggcc gcgaacaacg cgccccactc cgagctgaac tgggagcgga acacggagag
     2761 cccgatctgc gccgtgcgca tcctatcggt ggtcgtgacg acgaggggcc agaagaagtc
     2821 gtcccaagcg gtcacgaagg agaagatggc gatggtcgcg actatcggca gggatatcgg
     2881 tagatagaga ctggcgaaga cccgcacctc gctggcgccg tctatgcgcc cggagtccct
     2941 gatctcggcc ggaagatcta ggaagaactg ccgcgcgagg aagatcgtga acgtgagcac
     3001 taggtgcggc aacgcgatgc caagataagt gttcaacagg ccggttccgc ccgcccccat
     3061 gacgtcgttg ccgcccacga gcgggaagtt gcgggccagg atgaagagag ggatcgtcgt
     3121 gacatgcgac gggatcatca tggtgccgag cacgaccagg aacgcgaagt ccttgccggg
     3181 gatgggcagg atgaaggggt tggagccggt ggccagcatc aggcgatcgt attcggcggt
     3241 gaccaccgtg ccgtctttgc tggtggcgct gaccacgcgg acatcgacct gcacgcgctt
     3301 tgatcctcct
//
LOCUS       DAOQYE010000106         3304 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_30445_length_3304_cov_3.588181, whole genome shotgun sequence.
ACCESSION   DAOQYE010000106 DAOQYE010000000
VERSION     DAOQYE010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 3304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3304
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30445_length_3304_cov_3.588181"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            33..1262
                     /locus_tag="PKN52_02725"
     CDS             33..1262
                     /locus_tag="PKN52_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98885.1"
                     /translation="MTRTARRIARPSRIDRLVLRLVFTLLALPRSIAFAQLPAFLQVG
                     SRLTWNGGGSSLQGSRLVPDPNGWIWQNNQWNRVESTYGGGGVGLIQIDILSSSPEGV
                     VGDVRSYLNTDLQAGVYALSGVDAVVGDQSALSTYWVAPARLVALQTGWDGTTRVWRG
                     PRVINGAQYDTVSIATLAQGSYSSNTYDVATGLLLFGGTMDAQPGVMATDPNGTVVFQ
                     NQGAVSYSHQLFLGVRQLQVPWAGRPAPDWAAPGLTLVYQGQSRAELGGGGGLPPLPG
                     SAMAISYAFTQRLGSALLGYQVSQLATTQGLPPDQVTLARAFGSAAMDGLWVAPDVLG
                     GLQPGQVIDQDPFTGMQTYFGGVQNGGAVIVYQGRGDMLENYYDPNSGFLVFTRYRKQ
                     AANVGFQVNELWYTGTQ"
     gene            complement(1270..1524)
                     /locus_tag="PKN52_02730"
     CDS             complement(1270..1524)
                     /locus_tag="PKN52_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98886.1"
                     /translation="MEAEGAGATEVSNHAEEGDVLPPEARLDLASLSGEALTELCASL
                     ERMVTSGSAPLATYGELALAQAEVRRRESGAFPPNEVGPA"
     gene            1662..>3304
                     /locus_tag="PKN52_02735"
     CDS             1662..>3304
                     /locus_tag="PKN52_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98887.1"
                     /translation="MLSVRPTATPYHLRRSRLLERLPDEPGFTVWLEAPYGYGKSVLA
                     GQWAEELESDGWRVAWLSLAGRDPRPLLAQQLALPTTAPWGVIDEELRRGPTLLVLED
                     LQGTEDLTPIFQNHPGLVVLASRGPLPYPELPKLRTQGRLLHLTSEQLAFEESEAAEL
                     FEDSQRGREAWRLTQGWSLPLHFAALTGDLPQQATLLEGVKRSVSERAWHELMFLATV
                     DFLPKQAAVAATGDLARSGFLQTLTDGYRLHPLISESVLGAHASEVHAVLADQAHRLE
                     PALRGAAFETANFLEGLSGLLVTGDGAVALGTPEAFLRWNTLVPPSPEPARRARVTLA
                     RLALGRFDEALPDARELTTSGALTPQLRSLIASQALYGLGGVKRFEQAAEFSAVANRL
                     LAELAPQEAGAILRALGLVAFARGDYPEAERTFRAGLAKLQEVEPGRQRTVMELITRA
                     NLASTLWELHGDPHELLALVRELASTPDLDDGAFVTFNQNLAVAHAFVGDEASALAAT
                     RLAATKARDYGAFVVRSMLAYFEGDLTAFPALLAGARKW"
BASE COUNT          534 a         1191 c         1088 g          491 t
ORIGIN      
        1 ggtcaacgcc gccagaaccc ttagcatgtg ccatgacccg caccgcgcgt cgcatcgcca
       61 ggccctcgcg cattgaccgg ctcgtcctgc gtctcgtctt caccctcctc gctctcccga
      121 gatccatagc tttcgcccag ctgcccgcgt tcctccaagt aggttcgcga ctcacgtgga
      181 acgggggcgg ctcgagcctg cagggcagcc gcctggtacc cgacccgaac ggctggatct
      241 ggcagaacaa ccagtggaac cgggtggaga gcacgtacgg cggcggcggt gtcggcctga
      301 tccagatcga catcctgtcc tccagccccg agggtgtcgt cggcgacgtg cgctcctacc
      361 tcaacaccga cctgcaggcc ggggtctacg ccctgagcgg cgtcgacgcg gtggtcggcg
      421 accagagcgc cctgagcacg tactgggtgg cgccggcgcg gctggtggcg ctccagaccg
      481 gctgggacgg cacgacacgc gtgtggcgcg gaccgcgggt catcaacgga gcccagtacg
      541 acaccgtttc catcgcgacg ctggcacagg ggagttactc gagcaacacc tacgatgtcg
      601 ccaccggcct gctgctcttc ggcggcacga tggacgccca accgggcgtc atggccaccg
      661 atccgaacgg gacggtggtg ttccagaacc agggagccgt cagctacagc caccagctct
      721 tcctcggcgt tcggcagttg caggtgccgt gggccggccg accggcgccc gactgggcag
      781 cacccgggtt gacgctcgtg taccagggcc agagccgcgc ggaactcggg ggtggcggcg
      841 ggctgccgcc gctgcccggc tcggcgatgg ccataagcta cgcgttcacc caacgcctcg
      901 gcagcgcgct cctcggctac caagtctccc aactagccac cacgcagggg ctgccgcccg
      961 accaagtgac cctggcgcgc gccttcggca gcgccgccat ggacggccta tgggtggcgc
     1021 ccgatgtgct cggtgggctc cagcccggcc aggtcatcga ccaggacccg ttcacgggca
     1081 tgcagacgta cttcggcggg gtgcagaacg gcggagccgt catcgtgtac cagggccggg
     1141 gagacatgct cgagaactac tacgacccca actctggctt cctggtcttc acccgctacc
     1201 ggaaacaggc ggcgaacgtc ggcttccaag tgaacgagct gtggtacacg gggacgcagt
     1261 gacgtccgcc taggccggac cgacctcgtt gggcgggaag gcgcccgact cacggcgccg
     1321 cacctccgcc tgcgccaacg ccaactcgcc gtacgtggcg agcggcgcgg agccgctggt
     1381 gaccatgcgc tcgagactcg cgcacaactc ggtgagagcc tcaccgctca gggaggccag
     1441 gtcgagccgc gcctcgggag gtaacacgtc accctcctcg gcgtggttcg acacttcggt
     1501 ggcacccgcc ccttccgctt ccatgtcgag caggctacag ccgcgccgct taatactgac
     1561 ttaagagcac cgtcagttcg gtcctgacac agtgagccca gggggcgccc gctatactcg
     1621 gccacaccaa gtagcgcaag cgccaaccgc ctggagcgag aatgctcagt gtccggccca
     1681 cggcaacccc ttaccacctg cgccgcagca gactcctgga gcgcctgcct gacgagccgg
     1741 gcttcacggt gtggctcgag gcgccttatg gctacggcaa gagcgtcctg gccggacagt
     1801 gggccgaaga gctcgaaagc gacggttggc gagtcgcgtg gttgtctcta gccgggcgcg
     1861 acccgcgccc gctgctcgcc caacagctcg cgctgccgac aaccgcgccg tggggcgtca
     1921 tcgacgagga gttacgacgc gggccgaccc tcctggtgct cgaagacctg cagggcaccg
     1981 aggatctgac gccgatcttc cagaaccacc ccggcctggt ggtgctcgcc agccgtgggc
     2041 cactccccta ccccgagctg ccgaagctgc gtacgcaggg gaggctcctc cacctcacca
     2101 gcgagcagtt ggccttcgag gagtcggaag ccgccgaact cttcgaagac tcacagcggg
     2161 gccgagaggc ctggcgcctc acccagggct ggtcgttgcc gctgcacttc gcggcgctca
     2221 ccggcgacct cccccagcag gccacgctgt tggaaggcgt caaacggagc gtcagcgaac
     2281 gcgcctggca cgagctgatg ttcctcgcca ccgtcgactt cctgcctaag caggctgccg
     2341 tcgcggcgac cggcgacctc gcgcgctcgg gcttcctcca gaccctgacg gacggctaca
     2401 ggcttcaccc tctcatatcc gagagcgtcc ttggcgcgca cgcctcggaa gtccatgcgg
     2461 tcctcgccga ccaggctcac cgcctagaac cggccttacg gggcgcggcc ttcgagaccg
     2521 cgaacttcct cgaggggctg agcgggctcc tcgtgaccgg agacggcgcc gtggcgcttg
     2581 gcaccccgga agcgttcctg agatggaaca cgctcgtccc gcccagcccg gaacctgcgc
     2641 ggcgcgcgcg ggtcacgctc gcgcgcctgg cgctcggacg cttcgacgaa gccttgccgg
     2701 acgcgagaga actcactacg agcggcgcgc taacaccgca gctcaggtcg ctcatcgcca
     2761 gccaggcgct ctacggtctc ggtggagtaa aacgcttcga gcaggccgcg gagttcagtg
     2821 ccgtcgccaa ccgactcctg gcagagctgg cccctcaaga ggcgggcgcg atcctgaggg
     2881 cgctggggtt ggtcgccttc gcgcgcggcg actatccgga ggcggaacgc accttcaggg
     2941 ccgggttggc caagctccag gaggtagagc cgggtaggca gcgaacggtc atggagttga
     3001 tcacgagggc caacctcgcc agcaccttgt gggaactcca cggcgacccg cacgagctgc
     3061 tggccctggt ccgcgagctc gcctccacgc ctgacctgga cgacggcgcg ttcgtgacgt
     3121 tcaatcagaa cctggccgtc gcccacgcgt tcgtcgggga cgaggccagc gcgctcgcgg
     3181 ccacgcgact cgcggccacg aaggcccgcg actacggggc gttcgtggtc cgctccatgc
     3241 tcgcctactt cgagggcgac ctcaccgcct tcccggccct gctggccggt gcgcgtaaat
     3301 ggga
//
LOCUS       DAOQYE010000107         3303 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_30460_length_3303_cov_3.476293, whole genome shotgun sequence.
ACCESSION   DAOQYE010000107 DAOQYE010000000
VERSION     DAOQYE010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 3303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3303
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30460_length_3303_cov_3.476293"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1182
                     /locus_tag="PKN52_02740"
     CDS             <1..1182
                     /locus_tag="PKN52_02740"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNQ98888.1"
                     /translation="ERTAVSARYFLDATELGDLLELGGVEHVIGAEGQAETGEPNALP
                     EADPLNQQSHSWCFPLSYYPGEDHTIERPERYSFWSGHYLPGWPEAQLSWTVVHPITL
                     EPQTRPIFVGDTDAPNINDLWHFRRILYRRYYPEGEFASDVSVANWPQLDYTLGPLTG
                     VSPAERARHMQGAKELSLSMLYWMQTEAPRHDGGAGYPGLKLRPDVSGTKDGLAIYPY
                     IREARRVRAEFTVLEQHVSKACRERLGGAAVFHDSVGTGSYRIDLHPSTRGRNFVDIS
                     NYPFQIPLGALLPQRVENLLPAGKNIGTTHVTNGCYRLHPVEWNVGEVAGALAAFCLR
                     GGHAPRQVRNGLERLREFQGLLRTTFDIPLEWPDEVRRLPRDLPFGTSDAYGRTGAEV
                     I"
     gene            1205..1906
                     /locus_tag="PKN52_02745"
     CDS             1205..1906
                     /locus_tag="PKN52_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98889.1"
                     /translation="MGSLTETLRSERPTLCVSLIRNDARLAKEVQSAGADGIKVHINL
                     DHPYAKVRLGSFEQEAEALAELLAAVDVPVGIVPRGAPGTSVEEVESYAEFGFGFVDL
                     YAHVMNAALLAVPGIEKWAAPKGHFTAPMLRALATTGGVDVIEASFLPPAEFGAPLTV
                     EDLACLRAGLAAIDGAAPLVLPTDRRLTVEDLPVLIDNGVGNFLIGFAVTGSEPDDVV
                     RATERFRRAIESAKG"
     gene            1911..2678
                     /locus_tag="PKN52_02750"
     CDS             1911..2678
                     /locus_tag="PKN52_02750"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HNQ98890.1"
                     /translation="MDQVRIGLQLFSLRDELAADMEGTIRRVADIGYAGVEPFGLTPE
                     TATRTRRLCDELGLAVPSVHARMPEGEAVGAVMDTVAALAPERVVCTLGKAEFGSREA
                     VEKVCDRFNAANELLIAQGLSFGVHNHWWEFEAVDGVTPYRVMLERLDPRVFFELDVY
                     WMKTAGVDPAAVMSEFRGRAHLLHVKDGPAEVGRPMVALGQGAIDLPGIVAANAAATR
                     WLIVELDVCETDMFEAVAESHSYLEKLVSSMSTSAAQ"
     gene            2740..>3303
                     /locus_tag="PKN52_02755"
     CDS             2740..>3303
                     /locus_tag="PKN52_02755"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNQ98891.1"
                     /translation="MQGDDRGDLGVGRVEAGRVEAGRVGVGKIGVGRVGVGVIGCGTI
                     SDVYLKNLSRSESARVVAVADLDVERAKQKAEQYGVTAAATVGELLALSGVDLVLNLT
                     VPNAHADVALQCLRAGKSVYSEKPLATSFDDGALVLAEAGRRGLRVGCAPDTFLGSGQ
                     RTCRELIAGGAIGTVVGAVAFMANHGME"
BASE COUNT          552 a         1062 c         1168 g          521 t
ORIGIN      
        1 gagcgcacgg cggtcagcgc gaggtacttc ctcgatgcca ccgagctggg ggacctgctc
       61 gagctgggcg gcgtcgagca cgtcatcggc gccgaggggc aggccgagac tggcgagccc
      121 aacgccttgc cggaggcgga cccgctcaac caacagtccc atagctggtg cttcccgttg
      181 agctactacc ccggtgagga ccacacgatc gaacgaccgg agcggtactc cttctggagt
      241 gggcactact tgcccggctg gccggaagcg cagctcagtt ggacggtcgt ccatcccatc
      301 acgctggagc cgcagacgcg cccgatattc gtcggcgaca ccgacgctcc caacatcaac
      361 gacctctggc acttcaggcg catcctctac cggcggtact acccggaggg agagttcgcc
      421 agcgacgtga gcgtggcgaa ctggccgcaa ctcgactaca cgctcggacc tctgacgggc
      481 gtctctcccg cggagcgggc ccgccacatg cagggtgcca aggagcttag cctctccatg
      541 ctgtactgga tgcagacgga ggccccgcgc catgacggtg gcgcgggcta ccccgggctc
      601 aagctcaggc cggacgtcag cgggacgaag gacgggctgg ccatctaccc gtacatccgc
      661 gaggcgcgcc gcgtccgcgc ggagttcacg gtcctggagc agcacgtcag caaggcctgc
      721 cgcgagcgcc tcggcggcgc agccgtgttc cacgactccg tcgggaccgg ctcgtaccgg
      781 atcgacctgc atccgagcac gcggggccgc aacttcgtcg atatcagcaa ctacccgttc
      841 cagatcccct taggagcctt gctgccacag cgggtcgaga acctactgcc agccggcaag
      901 aacatcggca ccacgcatgt gacgaacggc tgctaccgtc tgcaccctgt cgagtggaac
      961 gtaggcgagg tcgctggcgc gctggcagcg ttctgcctac gcggcggtca tgcccccagg
     1021 caagtccgta acgggctgga gcggctgcgc gagttccagg gcctcctgcg cacgaccttc
     1081 gacatcccgc tcgagtggcc ggacgaggtc cgacggttgc cgcgcgacct gcctttcggg
     1141 acgagcgacg cctacgggcg aaccggagca gaagtcatct gatgactaag gttaggagtc
     1201 gaccatgggc agtttgacag agacgttgag aagcgaaagg cccacgctct gcgtcagcct
     1261 catccgcaac gacgcgaggc tggccaagga agtccagagc gccggcgcag acgggatcaa
     1321 ggttcacatc aacctcgacc acccgtacgc caaggtcagg ctcggcagct ttgagcagga
     1381 agccgaagcc ctcgcagaac tgctcgccgc cgtcgacgtg cccgtcggga tcgtcccaag
     1441 gggcgcgcca ggcacgtcgg tcgaggaggt cgagagctac gccgagttcg gcttcgggtt
     1501 cgtcgacctc tacgcccacg tcatgaacgc ggcgctcttg gcggttccgg ggatcgagaa
     1561 gtgggctgcc ccgaaagggc atttcaccgc tccgatgctc agggcattgg ctaccaccgg
     1621 cggtgtcgac gtcatcgagg cgtcgttcct gccaccggcc gagttcggcg cgcctctcac
     1681 ggtcgaggac cttgcctgcc tgcgcgccgg gcttgccgcc atcgacggag ccgcaccgct
     1741 ggtcttgccc accgatcgcc gtctcacggt ggaagacttg cctgttctga tcgacaatgg
     1801 ggtgggcaac ttcttgatcg gcttcgccgt gacgggtagc gagccggacg atgtggttcg
     1861 agccacggag cgtttccgtc gcgccatcga gtctgcgaaa gggtgagttg atggaccagg
     1921 ttcgcatagg actgcagttg ttctcgctcc gcgacgagtt ggcggcggac atggaaggga
     1981 ccatccgccg cgtggcggac atcggttacg ccggcgtcga accgttcggc ctcacgccgg
     2041 agacggcgac cagaacccgg cggctctgcg acgaactcgg cctggcggtg ccgagcgttc
     2101 acgcgcgcat gccggagggc gaggcggtgg gtgccgtcat ggacacggtc gccgcgctgg
     2161 caccggagcg ggtcgtgtgt acgctaggca aggccgagtt cggatcgcgc gaggcggtcg
     2221 agaaggtatg cgatcgcttc aacgctgcca atgaactctt gatcgcgcag gggctgtcgt
     2281 tcggcgtcca caaccactgg tgggagttcg aggccgtcga cggcgtgacc ccgtaccgcg
     2341 tcatgctcga gcgactggac ccgcgggtgt tcttcgagct cgacgtctac tggatgaaga
     2401 cggccggcgt cgaccccgcc gcggtcatgt cggagttccg tggcagggcg cacctgcttc
     2461 acgtcaagga cgggccggcc gaggtcggca ggccgatggt ggcgcttggg cagggggcga
     2521 tcgacctccc cggcatagtc gcggccaacg cggccgccac gcggtggctc atcgtcgagt
     2581 tggacgtctg cgagacggac atgttcgagg ctgtggcgga gagccattcc tacctggaga
     2641 agctcgtttc gagcatgagc acgagcgccg cacagtgaac ggtggcgcgg gtaggcggcg
     2701 gacgcgggtc gcgagcgcgg aagtttgaga ggggcgggca tgcaaggtga cgacaggggc
     2761 gacctcggag tcggcagggt cgaagccggc agggtcgaag ccggcagggt cggggtcggc
     2821 aagatcgggg tcggcagggt cggggtcggc gtgatcggct gcggcaccat cagcgacgtc
     2881 tacctgaaga acctttctcg ctccgagagc gcgagagtgg tggcggtcgc cgacctggac
     2941 gtcgagcggg ccaagcagaa ggccgagcag tacggcgtga ccgccgcggc taccgtcggc
     3001 gagttgctcg ccctgagcgg cgtcgacctc gtcctcaacc tcacggtgcc gaacgcgcac
     3061 gccgacgtgg cgctccagtg cctgcgggca ggcaagtccg tctactcgga gaagccgctt
     3121 gcgacgtcgt tcgacgatgg cgcgctcgtg ctcgccgagg cgggcaggcg tggtctacgc
     3181 gtgggctgtg cgccggacac cttcctcggc agcggtcagc gcacgtgccg agagctcatc
     3241 gcgggcggtg ccatcggcac ggtagtcggg gccgttgcct tcatggctaa ccacgggatg
     3301 gag
//
LOCUS       DAOQYE010000108         3294 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_30587_length_3294_cov_2.820315, whole genome shotgun sequence.
ACCESSION   DAOQYE010000108 DAOQYE010000000
VERSION     DAOQYE010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 3294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3294
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30587_length_3294_cov_2.820315"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..1214
                     /locus_tag="PKN52_02760"
     CDS             3..1214
                     /locus_tag="PKN52_02760"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HNQ98892.1"
                     /translation="MKDLLVLDLTRVLAGPYCTLLLADLGARVIKVEAPAGDDTRRWG
                     PPYPGGESGNYLSVNRGKESVVVDLKDERGAAMVRELARKADVIVENFKVGDLERYGL
                     DYASVAAANPRVVYVSITGFGQDGPRATEPGYDAAMQAQSGLLAMTGEPGGPPIKLGV
                     AWIDVLTGLHAATGALAALHRRERTGEGARLDVSLFDVALASLVNQAQNALLTGEAPP
                     RLGSAHPTIVPYQSFDAADGGVVVAVGNDAQFARLCVVLGTEALAHDPRFATNDGRVA
                     NRSVIVPLLARAIGERQREEFLAACRAAGVPATPIATLPDALADEQAVARGMVVQGAH
                     PTIGELPMVASPLWHALGPDGGPLGLAPTPGEAPVPPLLGQHTRLVLERELGLRAAEI
                     DALAAAGVVKG"
     gene            complement(1294..1938)
                     /locus_tag="PKN52_02765"
     CDS             complement(1294..1938)
                     /locus_tag="PKN52_02765"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HNQ98893.1"
                     /translation="MDHSSDTRPLPPAQARVYQRLLEHARRTGSPPDLAEFARELGVH
                     YVSLRQHLEALNTKGYLRFESRGRGRSPSLELPALATGIPLLGEIPAGPLAIATAHAE
                     AYLPAAAGGGRAESLFALRVHGNSMADLLQNDDVVLLAQRQPQRGGEICAVRVGEEEV
                     TLKYLDRLGGGRFALRPHNPDYPTVEVAGEQLAVDGVYRGLLRGTVAEALLLRE"
     gene            2154..2837
                     /locus_tag="PKN52_02770"
     CDS             2154..2837
                     /locus_tag="PKN52_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNQ98894.1"
                     /translation="MLDTFERLDTTSTCQVASIVPTGLALPTRTLEPGQVLYEADREA
                     SSLYIVNQGVLKAVVPTSLGRDRIADLYGPGDVLGLSSLDGGNHAETVVAVHDACLTP
                     IDPQQAMNDRGLRDYVLRSLARQLRRSREMLDEAEMPVGARVTRALLRLAKQFGLPAD
                     DQQGGIKLPLALTHEDLADLTGSSRVTITRILGELRSEGALSGTRGVYVADVPGLERA
                     TDHYVMQVL"
     gene            2859..>3294
                     /locus_tag="PKN52_02775"
     CDS             2859..>3294
                     /locus_tag="PKN52_02775"
                     /inference="COORDINATES: protein motif:HMM:NF013671.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HNQ98895.1"
                     /translation="MLKFTDIAKERVLRFLDAQSSQGMRALRIAGNRAEQRLWLVKAE
                     DGRPDDRVQAEAGFEVFLDPLTAAQLDGAVVDFVEGVMQSGFRIYHPSPSWDDPLAQR
                     VQDVIDKHINPGVAGHGGTVTLEGVEDGMAIIRFGGGCHGCGA"
BASE COUNT          496 a         1160 c         1175 g          463 t
ORIGIN      
        1 tgctgaaaga cctcctcgtc ctcgacctca cgcgggtcct ggccggtcct tactgcaccc
       61 tgctgttggc cgacctcggc gcgcgcgtta tcaaggtcga ggcgccggcg ggcgacgaca
      121 cgcggcggtg ggggccgccc taccccggcg gcgagagcgg gaactacctc tcggtcaacc
      181 ggggcaagga gagcgtcgtc gtcgacctca aggacgagcg tggggccgcc atggtccgcg
      241 agctggcgcg caaggccgac gtgatcgtcg agaacttcaa ggtcggcgac ctggaacgtt
      301 acgggctcga ttacgcgagc gtggccgccg ccaacccacg cgtcgtctac gtgtccatca
      361 cgggcttcgg ccaggacggc ccgagggcga cggagccggg ctacgacgcc gccatgcagg
      421 cgcagtccgg gctcctcgcc atgacgggcg agccgggcgg gccgcccatc aagctcggcg
      481 tggcctggat cgacgtgttg accggcctgc acgccgccac cggcgcgctg gccgcgctcc
      541 accggcgcga gcggacgggc gagggcgcgc ggctcgacgt gtcgctcttc gatgtggcgc
      601 tggcgagcct ggtcaaccag gcccagaacg cgttgctgac aggggaggcg ccgccgcgcc
      661 tcggcagcgc ccacccgacg atcgtgccgt accagtcgtt cgatgcggcg gacggcggag
      721 tggtggtagc ggtcgggaac gacgcgcagt tcgcgcgcct ctgcgtggtc ctaggcaccg
      781 aggcgctggc tcatgacccg cgcttcgcga cgaacgacgg gcgcgtagcg aaccgttccg
      841 tcatcgttcc gctgctggcg cgcgctatcg gcgagcgcca gcgcgaggag ttcctggccg
      901 cctgtcgggc ggccggcgtg ccggccacgc ccatcgcgac gctgcccgat gcgctggcgg
      961 acgagcaggc cgtggcgcgt ggcatggtcg tccaaggggc gcatccgacc atcggagagt
     1021 tgccgatggt agcgagcccg ctgtggcacg cgcttggccc cgacggcggg ccgctcggac
     1081 tggcgccaac gccgggcgag gcgcccgtcc cgccgctcct gggccaacac acgcggctcg
     1141 tgttggagcg cgaactcggt ctgcgggccg ccgagatcga cgcgctggcg gccgcggggg
     1201 tcgtgaaggg ctgaggcgcg gtgcgacacg gcatgcggtg gccaccggac ggtggtcgct
     1261 gcggcgcgcc gcgtacgcgg cgctggcatg ggctcactcc ctgagcagca acgcctccgc
     1321 caccgtgccc ctgaggaggc cgcgatagac gccgtcgaca gccagttgct cgccggccac
     1381 ctcgaccgtc ggatagtcgg ggttgtgcgg ccgtagcgcg aagcgaccgc cgcccagccg
     1441 gtcgaggtac ttcaaggtca cttcctcctc gccgacccgc acggcgcaga tctcgccgcc
     1501 gcgctgcggc tgccgttggg cgaggagcac gacgtcgtcg ttctgtagca ggtcggccat
     1561 ggagttgccg tggactcgca gggcgaagag cgactcggcg cggccgccgc cggccgcagc
     1621 gggaaggtac gcctccgcgt gggcggtggc gatggcgagg ggtccggccg ggatctcgcc
     1681 gagcagcggg atgccggtgg ccagggcagg aagctcgagc gacggggagc gcccacgccc
     1741 gcggctctcg aagcgcaggt aacccttcgt gttcagagcc tccaggtgct ggcgcagcga
     1801 gacgtagtga acgccgagct cccgcgcgaa ctccgccaga tcgggtgggg agcccgtgcg
     1861 gcgcgcgtgc tcgaggaggc gttggtacac ccgtgcctgg gccggcggca gtgggcgggt
     1921 gtctgatgaa tggtccatga ctacctaaaa tgctaaggta gtgaccgcgg cttgtcaacc
     1981 ggccgcgagg ccgcgcatta agcccgtcca gaacggttct aatgcgtttc tcaccccaga
     2041 ggcggcctgt ggacaaagcc ggggcgccct gctatagttt ctgtagttga gcgcacccac
     2101 ctcacccggg cgggccgccc gggaccgcgc cgactcgaaa ggaaccacga ataatgctcg
     2161 acacgttcga acgactcgat acgacgagca cctgtcaggt agcaagcatc gtgcccaccg
     2221 gtctggcgtt gccgacgcgc acgctcgagc ccggtcaggt gctctatgag gccgaccgcg
     2281 aggccagcag cctctacatc gtcaaccagg gcgtcctcaa ggcggtcgtc cccaccagcc
     2341 tcggccgcga ccgcatcgcc gacctctacg gcccgggaga cgtgctgggc ctgtcgtcgc
     2401 tcgacggcgg caaccacgcc gagaccgtcg tggccgttca cgacgcctgc ctcacgccca
     2461 tcgacccgca gcaggcgatg aacgaccgcg gcctccgcga ctacgtgctg cgcagcctcg
     2521 cgcgccaact gcgccgcagc cgcgagatgc tcgacgaggc cgagatgccc gttggcgccc
     2581 gcgtcacgcg cgcgctcctg cgcctcgcca agcagttcgg cctgccggcc gacgaccagc
     2641 agggcggcat caagctcccc ctcgcgctga cgcacgagga cctcgccgac ttgacgggct
     2701 ccagccgcgt gaccatcacg cgcatcctcg gggagctgcg cagcgagggc gccctgtccg
     2761 gtacccgggg tgtgtacgtc gccgacgtgc ccggtctcga gcgcgccacc gaccactacg
     2821 tgatgcaggt tctgtagcga acggagtacg cttcggtcat gctgaagttc accgatatag
     2881 ccaaggaacg cgtcctccgc ttcttggacg cccagagcag ccaaggcatg cgcgctctgc
     2941 gcatagccgg taaccgcgcc gagcagcgcc tctggctcgt caaggcggaa gacggacgac
     3001 cagacgaccg ggtccaggcg gaagccggct tcgaagtgtt cctcgacccg ctcaccgccg
     3061 cccagttgga tggcgccgtc gtcgatttcg tggaaggcgt catgcagtcg ggcttccgca
     3121 tctaccaccc gagcccgagc tgggacgacc cgctcgcgca gcgcgtgcag gacgtcatcg
     3181 acaagcacat caaccccggc gtggcgggcc acggtggcac cgtcacgctc gagggcgtcg
     3241 aggacggcat ggccatcatc cgcttcgggg gtggctgcca cggctgcggc gccg
//
LOCUS       DAOQYE010000109         3283 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_30746_length_3283_cov_3.065056, whole genome shotgun sequence.
ACCESSION   DAOQYE010000109 DAOQYE010000000
VERSION     DAOQYE010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 3283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3283
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30746_length_3283_cov_3.065056"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..770)
                     /locus_tag="PKN52_02780"
     CDS             complement(<1..770)
                     /locus_tag="PKN52_02780"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HNQ98896.1"
                     /translation="MTASDILTLRPSASQTAGGAFSASAFAGHDPTGADPTAPTGEDP
                     AGVKFSGNEFAGSAIEGRHFRAALSRFTTGITVVTMLGAAPEPPGPTTSDPTASEPGA
                     QAQTASEPKALEPVAPERGAHEHAAGERAGPFGITVNAFLSLSLEPPLVAVSIDKRAR
                     AHATLVRSERFGVSVLAADQADVSDHFAGRPVALNGVPYEEYEGFPVIRGAIAQLVLR
                     TEQQVDVGDHTLFVGRVEALRYADGAPLVYFGSAYRRLP"
     gene            862..>1173
                     /locus_tag="PKN52_02785"
     CDS             862..>1173
                     /locus_tag="PKN52_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98897.1"
                     /translation="MVGSEFKSEGPYDTRSGAPLGALLEDRRLGLSLVVGDPTKRFLN
                     VRWPEREPDRRWLEPGDLRLVGAGAGSSGSFEPLLRSRNLRRARRILREGTDAALQTD
                     PA"
     assembly_gap    1174..1183
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1184..2545
                     /locus_tag="PKN52_02790"
     CDS             <1184..2545
                     /locus_tag="PKN52_02790"
                     /inference="COORDINATES: protein motif:HMM:NF037653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNQ98898.1"
                     /translation="VYALTPSDRRLPDGLATRAQAAGVTLLLAPPSTAPERVEAAALR
                     LLAAVPGAAAVHAPQAFLLAALEGAKPERDVLERLHELTDADLVLLGAAGEVVARAGQ
                     GTWRPRSETPIGAWTEGRVRLDRKDAFLYRVSPQGRLRTILLVFPAGRLDPRPWAEFA
                     RTLLATAYDRRASEALAVRTERAGLLALWLAAPTATGLGTRLAAHGFGPRTPYRVAVG
                     EVAPPTAGAATSGATRAAARGTGVGASEQDARASAAGCDHFAERAVPVLSEVRAGRVV
                     WLFADTGSSSPESSPHAHALLRAMAEAWRGETGVDNGPSAPRLGISEVVEGVAAAPEA
                     YRRAALALEQAAPGSVEAYEPDVVDELLRHQTPEQLRALRETVLGTLAAGDPRGRLRA
                     TLDAYLRNRHDLPSLAAELGVHVNTLRYRLKRVEELLGAQLTDPETIAKVWLATRRSA
                     GDA"
     gene            2538..3014
                     /locus_tag="PKN52_02795"
     CDS             2538..3014
                     /locus_tag="PKN52_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNQ98899.1"
                     /translation="MRDIPPGASARHDLVVTEAMTVDFEDRYDPRLGRLHPVYATYWL
                     VKHMELVGRKLVLPFLEDDEEGIGHAVSVRHTASALPGMRVSLRARFERVEGNRVYVS
                     CVATSELGDTVGEGETVQVVLKRAVIEAGFARLRRRWGADVVEEGAPPRGPADEQV"
BASE COUNT          482 a         1143 c         1199 g          449 t
ORIGIN      
        1 ggcagccgcc ggtaggcgct gccgaagtag acgagcggcg cgccgtcggc gtagcggagg
       61 gcctcgaccc taccgacgaa gagcgtgtga tcgccgacgt cgacctgctg ctcggtgcgc
      121 aggacgagct gcgcgatggc gccccggatg acggggaagc cctcgtactc ctcgtacggt
      181 acaccgttca gcgccaccgg ccggccggcg aagtgatcgg agacgtccgc ctggtcggcc
      241 gcgaggaccg agacgccgaa gcgctccgac cggaccagcg tcgcgtgcgc cctggcgcgc
      301 ttgtcgatgg acacggccac cagcggcggc tcgagcgaca aggacaggaa ggcgttgacg
      361 gtgatgccga atggcccggc ccgctcccct gcggcgtgtt cgtgggcgcc gcgttcgggt
      421 gcgacgggtt cgagcgcctt aggttcggac gccgtctgtg cctgcgctcc tggttcggaa
      481 gccgttgggt cagacgtcgt cggtcccggt ggttctggcg cggcgccgag catggtaacg
      541 accgttatcc cggtcgtgaa ccggctgagc gccgcccgga agtggcgacc ttcgatggct
      601 gagccagcga actcgttgcc ggaaaactta acgccggcgg ggtcctcgcc ggttggagcc
      661 gtcggatcag cgccggttgg atcatggccg gcgaacgcgc tggcggagaa cgcaccgcca
      721 gcggtctgac tcgccgaggg ccgtagtgtg aggatgtcgg aagcagtcac cgaatcgaca
      781 ggttagcgcc ctgccaccta atcaccaatt gtttagatgc acaatcgctt agctataatt
      841 gtacaggtgt acaatctggc cgtggtaggt tccgagttca agtcggaggg cccgtacgac
      901 accagatcag gcgcgccgct cggcgcgctg ctcgaggatc gccgcctagg gttgagcctc
      961 gtcgtcggcg accccaccaa acgcttcctg aacgtccgct ggccagagcg cgaacccgat
     1021 cgccgctggc tcgagcccgg cgacctgcgc ctagtgggcg cgggcgccgg gagcagcggc
     1081 tcgttcgagc cgctgctgcg ctcgcgcaac ctgcgccggg cgcgccgcat cctgcgcgag
     1141 ggcaccgatg ccgcgctgca gaccgacccc gccnnnnnnn nnngtctacg cgctaacccc
     1201 ctccgaccgg aggctgccgg acgggctggc gaccagggcc caggcggccg gggtgacgct
     1261 cctactcgca ccgccctcca cggctcccga gcgggtcgag gcggcggcgc tacgcctact
     1321 cgctgcggtc ccgggtgcgg ccgccgttca cgcgccgcaa gcgttcctcc tggctgccct
     1381 ggaaggcgcg aagccggaac gcgacgtcct tgaacgcctt catgagctca ccgacgccga
     1441 cctggtgctc ctcggcgcgg cgggcgaggt cgtcgcccgc gcgggccagg ggacgtggcg
     1501 gccacggtcg gagacaccca tcggcgcctg gacggagggc cgcgtgcgtc tggacaggaa
     1561 ggacgccttc ctctaccgcg tgagcccgca gggcaggctc cggaccatcc tgctcgtctt
     1621 tccagccgga cggctcgatc cgcgaccgtg ggcggagttc gctcgcacgc tgctcgccac
     1681 ggcgtacgac aggcgcgcat cggaggccct cgccgtgcgc accgagcggg cgggcctcct
     1741 cgccctatgg ttggcggctc cgaccgccac cggtctgggc acccgcctgg cggcacacgg
     1801 cttcggccca cgcaccccgt accgcgtggc ggtcggcgag gtcgcgccac ccacagcagg
     1861 cgccgcgacc agcggcgcaa ccagagcggc ggcgagaggc accggggtcg gagcgagcga
     1921 gcaggacgca cgggcgagtg ccgccggctg cgaccacttc gcggagcgcg ccgtgcccgt
     1981 cctctccgag gtgcgcgccg ggcgcgtggt gtggctgttc gcggacacgg ggagttcgag
     2041 ccccgaaagc tcgccgcacg cgcacgccct cctgcgcgcc atggcggaag catggcgggg
     2101 cgagaccggc gtcgacaacg ggccgagcgc gccgcgcctg ggcatcagcg aggtggtgga
     2161 gggcgtggcg gcggcgcccg aggcgtaccg gcgcgcggcg ctggcgctcg aacaggccgc
     2221 tcccggcagc gtcgaggcct acgagcccga cgtcgtcgat gaactcctgc gccaccagac
     2281 gccggagcag ttgcgcgcct tgcgggagac cgtgctcggc acgctcgccg ccggcgaccc
     2341 gcgcggcagg ctgcgcgcca cgctggacgc ctacctgcgg aaccggcacg atctgccgag
     2401 cctggcggca gagctaggag tgcacgtgaa cacgctccgt taccgcctga agcgcgtcga
     2461 ggaactgctg ggggcccagc tgacggaccc ggagaccatc gcgaaggtgt ggctggccac
     2521 gcgcaggagc gccggcgatg cgtgacatcc cgcccggcgc ctcggcgcgc cacgacctcg
     2581 ttgtcaccga ggccatgaca gtggacttcg aggatcgata cgacccgcgg ctcggcagac
     2641 tccaccccgt ctacgcgacc tactggctcg tcaagcacat ggagctcgtc gggcgcaagc
     2701 tcgttctccc cttcctagag gacgacgagg agggcatcgg tcacgccgtg agcgtcaggc
     2761 acacggcatc ggcgctgcct ggcatgcgcg tctcgctcag agcgaggttc gagcgcgtcg
     2821 agggaaaccg cgtgtacgtg agctgcgtag cgacgagcga actcggggac accgtgggcg
     2881 agggcgagac cgtccaggtc gtgctcaagc gtgccgtcat cgaggcgggg ttcgcgcgcc
     2941 tgcggcgccg gtggggtgcg gacgtcgtcg aggagggggc gccgccccgc ggtcccgccg
     3001 acgagcaggt ctgacggacg ctccggtccg cgctcgttct cccggtcgtc tgtggcacgc
     3061 gcggtaggac cacgtggtcg ccaccgtaga atcgcgtctg gaggcgagga tggccaaggt
     3121 ccacctgcta ggcactgggg cggcgctgtc ggacaagagc aggaccacga cgatgctggc
     3181 cctcgagggg caagagagcc taatgctcgt cgactgcggc ggcgacgcgg cccatcgcct
     3241 cctcgccagc gacctcgatc tggccaaggt gagcggcctg atc
//
LOCUS       DAOQYE010000110         3280 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_30791_length_3280_cov_2.328062, whole genome shotgun sequence.
ACCESSION   DAOQYE010000110 DAOQYE010000000
VERSION     DAOQYE010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3280
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30791_length_3280_cov_2.328062"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /locus_tag="PKN52_02800"
     CDS             <1..624
                     /locus_tag="PKN52_02800"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNQ98900.1"
                     /translation="WGAVLGEAHDRATELRLAKLSLVQFTRAFAQAFEAVDFAQLGHA
                     ERVTAYALALGRAMGASRAQLADLYFAAMLHDIGKLGNGLDQKIEDLEHPQRGANMVA
                     ASDLLAPAAEGIRHHHEAWDGSGFPDGLRKEDIPLLARIVAVADSFDLLSSERGEALP
                     MRDVEKALDARAGRTLDPAVVALLMNVLRQGRSTQELSQLSDGDLPF"
     gene            739..1476
                     /locus_tag="PKN52_02805"
     CDS             739..1476
                     /locus_tag="PKN52_02805"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HNQ98901.1"
                     /translation="MSVEVMSFRYTYRGKPAGTQVIKTELKGRHAQLEGKAQFQGPLG
                     NATIVQRSRCSAERFFSLRYLEETQERNESRSFDVTFDQQTGLVSAIKGQKDQASIPY
                     LVAYRDPLSLLHELRSIGERSEPVAIPLLGKTVTVQFGGEAELDGPLGARRARAYVIH
                     PGQSVVYVDTQAPHHILKLTQRVADGHLDALLVKVASEGALEPFGDAPARTGRRRGGG
                     SAGNGGKGQDKGEQRGSRRRPRRRRRA"
     gene            1479..2885
                     /locus_tag="PKN52_02810"
     CDS             1479..2885
                     /locus_tag="PKN52_02810"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HNQ98902.1"
                     /translation="MGATPDDLTRSDTEGDPGGYAAALQWLYSRTRSGGKRTSARAAK
                     LLRELGLAPPPVTAVVVGTNGKGTVSAMLARGLSAAGARTGRFLSPHVEEFRERVAID
                     GVPVSKAALVAFVERARRLDDAWPHGEDLRPAFFEWTLAFALEQFAAATDACVLEAGV
                     GGLHDATRAVEPVGLVVLTNVDLDHMDTLGTTLTAIASDKAGAFRAGVPVVCGASQPE
                     VVAVAARSAAELGAPFHLDPYAAVQPPGSGGGARCAPVAGDEGLFELPRDVAADLAAS
                     PAGVASGALAGANANRRANSRLAAAALRLLGAREPAVAAGLRAPALPARFERFILPGE
                     HGRQLLVVLDGAHDPAAAARLASEVAPGYALLFGALARKQGQRVLDTLAPNASTVIVT
                     EAASGETPTARWPGARVVSDTAAALATAADAAHVAGAPLLLVAGSLYLAGLVRPLLTR
                     RGVRLSDPWERRAVEDRS"
     gene            2918..>3280
                     /locus_tag="PKN52_02815"
     CDS             2918..>3280
                     /locus_tag="PKN52_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98903.1"
                     /translation="MATFPDFGATAGAAAPGLSSAAGAGRVGFVSLGCPKALVDSERI
                     LSQLRVEGYAVVPGFDEADVVVVNTCGFITPAVEESLDAIGEALRENGRVIVTGCLGE
                     RPETIRERARLMYVTGAFP"
BASE COUNT          456 a         1215 c         1160 g          449 t
ORIGIN      
        1 tggggcgccg tcctcggcga agcccacgat cgtgccaccg aactccgcct cgccaagctg
       61 agcctcgtgc agttcacgcg cgcgttcgcg caggccttcg aggccgtgga cttcgcgcag
      121 ctgggccatg ccgagcgcgt gaccgcctac gccctcgcgt tgggccgcgc catgggcgcc
      181 agccgggcgc agttggccga cctctacttc gcggccatgc tccacgacat cgggaagctc
      241 ggcaacgggc tggaccagaa gatcgaggac cttgagcacc cgcagcgcgg cgcgaacatg
      301 gtagccgcca gcgacctact ggctccggcc gccgagggca tccgccacca ccacgaggcg
      361 tgggatggct cgggtttccc ggacgggctg cgtaaggagg acataccgct gttggcccgc
      421 atcgtggcgg tagccgactc tttcgacctc ctgagcagcg agcgcggtga ggcgctgccc
      481 atgcgcgacg tcgagaaggc cctggacgcc agggcgggca ggacgctcga ccccgccgtc
      541 gtggcgctcc tgatgaacgt cctgcgccaa ggcaggagca cccaggaact cagccaactg
      601 agcgacggcg acttgccctt ctgaccgccg tgcggccgcg gggtgcgaag cgccaggcgg
      661 gccgcgaccc aacgagttgc cgttctgaac ggtcccctgg cgcttcgcga cgccgcggag
      721 ggtggtacgc ttcagcccat gtcggtcgag gtgatgagct tccggtatac ctaccgcggc
      781 aaacctgccg gcacccaggt catcaagacg gaactcaagg gtcgccacgc gcagctcgaa
      841 ggcaaggcgc agttccaggg accccttggc aacgccacca tcgtccagcg cagccgctgc
      901 agcgccgagc gcttcttctc gctgcgctac ctggaggaga cccaggagcg gaacgagtcg
      961 cgcagtttcg acgtgacgtt cgaccagcag accgggctgg tctccgcgat caaggggcag
     1021 aaggaccagg ccagcatccc ctacctggtc gcataccgcg accccctgtc cctccttcac
     1081 gagctcaggt ccatcggcga acgctccgag cccgtcgcca tacccctcct tggcaagacg
     1141 gtgaccgtcc agttcggcgg ggaggccgag ttggacggac cgctcggtgc gcgccgagcc
     1201 cgcgcgtacg tgatccaccc agggcagagc gtagtctacg tcgacaccca ggccccccac
     1261 cacatcctga agctcacgca gcgcgtggcg gacgggcacc tcgacgccct gctcgtcaag
     1321 gtcgcgagcg aaggcgcgct ggagccgttc ggcgacgcgc ccgcgcgcac gggccgtagg
     1381 cgtggcggcg gcagcgcggg caacggcggc aagggccagg acaagggcga acagcgcggc
     1441 tcgcggaggc ggccgcgccg ccgccgccgc gcctgacggt gggagcgacg ccggacgacc
     1501 tgacgaggag cgacaccgag ggcgacccgg gagggtacgc cgccgctctg cagtggctct
     1561 actcgcgaac gcgctcgggt ggcaagcgca ccagcgcgcg agcggccaag ctcctacgcg
     1621 agctgggtct ggcgccgccg ccggtgaccg ccgtcgtggt gggaaccaac ggcaagggca
     1681 ccgtatccgc catgctcgcc cgggggctga gcgccgcggg cgcgcgcacc gggcggttcc
     1741 tgtcaccgca tgtggaggag ttccgtgagc gcgtggccat cgacggcgtg cccgtctcca
     1801 aggcggcgct cgtggcgttc gtggagcgcg ccaggcgtct cgatgacgcc tggccgcacg
     1861 gcgaggacct acgaccggct ttcttcgagt ggactctggc gttcgcgctc gagcagttcg
     1921 ccgccgcgac cgacgcctgc gttctcgagg cgggcgtcgg ggggctccac gacgctacgc
     1981 gcgctgtcga accggtcggc ttggtggtcc tcaccaacgt cgacctggat catatggaca
     2041 ccctcggcac caccctcacg gccatcgcct ccgacaaggc cggcgccttc cgcgctggcg
     2101 tccccgtcgt gtgcggcgcc tcccaacccg aggtcgtcgc cgtcgccgcg cgcagcgcgg
     2161 ccgaactcgg cgcgcccttc catctcgacc cgtacgcggc ggtccaaccg ccaggctccg
     2221 gcggaggcgc gcgctgcgca ccggtggcgg gcgacgaggg tctcttcgag cttccccgcg
     2281 acgtcgccgc cgacctcgcg gccagtccgg ccggcgtcgc gtccggcgcg ttggccggcg
     2341 cgaacgccaa ccggcgcgcc aactcgcgcc tggcggccgc ggcgcttcgc ctgctcgggg
     2401 cgcgcgagcc ggccgtagcg gcggggctgc gagccccggc gcttccggcc cgcttcgaac
     2461 ggttcatcct gcccggcgag cacggccgcc aactgctcgt ggtcctcgac ggcgcccacg
     2521 accccgcggc cgcggctcgg ctggcgtcgg aggtcgcccc ggggtacgcg ctcctattcg
     2581 gtgcgctggc gcgcaaacag ggtcagaggg tgctcgacac gctggcgccc aacgcctcca
     2641 ccgtgatcgt cacggaggcg gcgtccggcg agaccccgac cgcccgctgg cctggcgcca
     2701 gggtcgtgag cgacaccgcg gcggccctcg ctaccgcggc cgacgccgct cacgtcgcgg
     2761 gggcgcccct tctgctggta gccggctcgc tctacctcgc cgggctcgtg cgaccgcttc
     2821 taacgcgtcg gggcgtgcgc ctgtcggacc cgtgggagcg gcgggcagtc gaggaccgct
     2881 cgtagcgcac gaggctactg ttacactcgg cggcggtatg gcgaccttcc ccgactttgg
     2941 tgcaaccgcc ggcgcggcgg cgcccggcct ctcctcggcc gccggcgccg gccgcgtcgg
     3001 cttcgtcagc ctcggctgcc cgaaggcgct cgtcgactcc gagcgcatcc tcagccagct
     3061 ccgtgtcgag ggctacgcgg tcgttcccgg cttcgacgag gccgacgtcg tggtcgtcaa
     3121 cacgtgcggc ttcataacgc cggccgtcga ggagtccctc gacgccatcg gcgaggcgct
     3181 acgcgagaac ggcagggtga tcgtcactgg ctgcctcggc gagcgccccg agacgatcag
     3241 ggagcgggcc aggttgatgt acgtgaccgg ggcgttccca
//
LOCUS       DAOQYE010000111         3277 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_30835_length_3277_cov_3.291434, whole genome shotgun sequence.
ACCESSION   DAOQYE010000111 DAOQYE010000000
VERSION     DAOQYE010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 3277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3277
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30835_length_3277_cov_3.291434"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1197
                     /locus_tag="PKN52_02820"
     CDS             <1..1197
                     /locus_tag="PKN52_02820"
                     /inference="COORDINATES: protein motif:HMM:NF025620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding domain-containing protein"
                     /protein_id="HNQ98904.1"
                     /translation="EPRRPGGKNMTDFGGTTTSGRAPRAARPFTRLAKLLTAPILTLL
                     AACATLPAMAQPSTGATITFAEQATVDGDGATATGARVVITAGGTYVITGQASNGSVT
                     VDAAGADVTLVLSGLDLTNAEGPAIYVAAAGSATVTLAAGSSNQVADGGSSEYDAALY
                     SDAPLVIDGEGALDVHAVYEGISSTSHIDIVSGTIRIFASEDGINANQDGVSRITVSG
                     GYVYVQTDTGDGIDSNGTITITGGVVVTQAALVDANSGLDADGDVTIDGGVVVATGSQ
                     MMGSVATDSAQETIVVNYGASQAAGTLIVVRDEAGNDLLAFAPANGYQQLLFSSPALQ
                     DGVTYTVYSGGTPTGVGVDGLYAAGAADPGTAVATVTTESAQGGGGFGPGFGGPGAGR
                     GAPGRP"
     gene            complement(1340..2749)
                     /locus_tag="PKN52_02825"
     CDS             complement(1340..2749)
                     /locus_tag="PKN52_02825"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HNQ98905.1"
                     /translation="MSLTEQAALIRAGELSPVALVELYLDRIARYDSKIKAFITVFAD
                     SALERARLAEQEIRAGDYKGPLHGIPVGVKDQMQIEGKRVTGGSKIYADSICERDATV
                     IARLKAAGAILLGTLNTHEFHMGPTINFPYGTPRNPWNLERNPGGSSSGSATSLATGL
                     ISAALGGDTGGSIRGPSTACGVVGFKPTWSRVSRDGVFPLAWTLDCVGPLARTVEDVA
                     VVLQVIAGRDPLDPTTSSEPVPDYSAALEDASLKGLRVGVVEEMIKDDITGPDAIAAV
                     KKALEFFESRGAVLSSVSLPLMRETRFISPGLTKPEAAAYHRRNLLERYMDFDYNTRV
                     SSMVGAILPAGLANLAQRARIVIGQQVLDALQSVDVLVGAGSAGGAVPIVPRPPIKTK
                     QEALEKIYGVGKAAGQYTRVFSLAGTPAMVVPAGFDHEGLPLSLHIAGRLFDEATVLR
                     VGNAFQKATDWHGRRPPVA"
     gene            complement(2766..2930)
                     /locus_tag="PKN52_02830"
     CDS             complement(2766..2930)
                     /locus_tag="PKN52_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98906.1"
                     /translation="MLKQQGYVIPEEELVEVTASVNALIEGLTHLEGYGELQNEPWPV
                     LVGYLGGGHA"
     gene            complement(3046..>3277)
                     /locus_tag="PKN52_02835"
     CDS             complement(3046..>3277)
                     /locus_tag="PKN52_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98907.1"
                     /translation="FVPELGFDRLCDTVPAGTVLGRVYSPHTFELLEELTAPYDQSVV
                     IMLRGTLSRVNPGDYAYIICNGATAEPVVNGK"
BASE COUNT          520 a         1141 c         1119 g          497 t
ORIGIN      
        1 gaaccgcgcc gaccaggagg gaagaacatg accgacttcg gcggaacgac gaccagcggc
       61 cgggctccac gcgccgcccg cccattcacc aggctggcca agctcctcac ggcccccatc
      121 ctcactctgc tcgccgcctg cgccaccctg cccgccatgg cgcaaccctc gaccggcgcc
      181 accatcacct tcgccgagca ggcgaccgtc gacggcgacg gcgcgacggc taccggcgcc
      241 cgggtggtca tcacggccgg aggcacttac gtcatcacgg gccaagcgag taacggctcc
      301 gtgacggtcg acgcggcggg agcggacgtg acgctcgtcc tgagcggcct agacctcacc
      361 aacgcggagg ggcccgccat atacgtagcc gccgcaggct ccgctaccgt gacgctggcc
      421 gccggctcca gcaaccaggt cgcggacggc ggctcgagcg agtacgacgc cgccctctac
      481 agcgacgccc cgctcgtcat cgacggcgag ggcgcgctcg atgtgcacgc cgtctacgag
      541 gggatctcga gcacgtcgca catagacatc gtgagcggga ccatcaggat cttcgccagc
      601 gaagacggca tcaacgcgaa ccaggacggc gtgagccgca tcaccgtcag cggcgggtac
      661 gtgtacgtgc agacggacac gggcgacggc atcgactcga acgggaccat cacgatcacg
      721 ggcggggtgg tcgtcacgca ggcggctctc gtggacgcca acagcggcct cgacgccgac
      781 ggcgacgtca ccatcgacgg cggcgtggtc gtggcgacgg gcagccagat gatgggctcc
      841 gtcgcgaccg actccgccca ggagaccatc gtcgtgaact acggcgccag ccaggccgca
      901 gggactctga tcgtcgtgcg cgacgaggcc ggcaacgacc tcctcgcctt cgcgccggcc
      961 aacggctacc aacagctcct cttcagctcg cccgcccttc aggacggcgt cacctacacc
     1021 gtgtactcgg gcggcacgcc cacgggcgtc ggcgtagacg gtctctatgc tgctggagcc
     1081 gccgatcccg gcactgccgt agccaccgtc accacggaaa gcgcgcaggg cgggggcggt
     1141 ttcgggccag ggttcggtgg gccgggcgcc gggcgcggcg cgcctgggag gccatgaggc
     1201 cgtcggcgaa cccccgttga catgaccgac acggccccgc cctcggcaaa gtcaagcgcc
     1261 gagggcgggg ccgttgctgc gggtcgctgg tccgctcagc cggggccctt ggggcctgcg
     1321 caccttctct ccggcttact caagcgacag gcggccgcct gccgtgccag tcggtggcct
     1381 tctggaaggc gttgccgacg cgcagtacgg tggcctcgtc gaagagccgg cccgcgatgt
     1441 gcaggctgag cggcagaccc tcgtggtcga agccggctgg cacgaccatc gccggggtgc
     1501 cggccaggct gaacacgcgc gtgtactggc ctgcggcctt gcccacgccg tagatcttct
     1561 cgagcgcctc ctgcttggtc ttgatcggcg gacgagggac gatgggcacc gcaccgcccg
     1621 ccgaccccgc acctacaagc acgtccacgc tctgaagagc atcgagtacc tgctggccga
     1681 tgacgatcct cgcgcgctgc gccaggttcg ctaggccggc gggcaggatg gcgccgacca
     1741 tggagctgac gcgcgtgttg tagtcgaagt ccatgtagcg ctccaggagg ttgcgccgat
     1801 ggtacgcggc cgcctccggc ttggtgagtc caggcgagat gaagcgcgtc tcgcgcatca
     1861 gcggcaggct cacgctcgac aggaccgcgc ccctcgactc gaagaactcc agcgccttct
     1921 tcacggccgc gatagcgtcg gggccggtga tgtcgtcctt gatcatctct tcgacgacgc
     1981 cgactcgcag gcccttcaac gaggcgtcct ccaaggcggc cgagtagtcg gggacgggtt
     2041 cgctgctggt cgtcgggtcg agcggatcgc ggccggcgat gacctgcaag accacggcga
     2101 cgtcctcgac cgtccgggcg aggggtccga cgcagtcgag ggtccaggcg agggggaaga
     2161 cgccgtcgcg gctgacccgc gaccaggtgg gcttgaagcc cacgaccccg caggcggtcg
     2221 acggtcccct gatcgaaccg cccgtgtcgc cgccgagcgc cgcgctgatc agtccggtcg
     2281 ccagcgacgt ggccgaaccg ctggacgaac cgcccgggtt gcgctccagg ttccaggggt
     2341 tgcgtggcgt gccgtacggg aagttgatgg tcgggcccat gtggaactcg tgggtgttga
     2401 gggtgccgag caggatggcc ccggccgcct tgagcctggc gatcacggtg gcgtcgcgct
     2461 cacagatcga gtcggcgtag atcttcgacc cgcccgtcac gcgcttaccc tcgatctgca
     2521 tctggtcctt gacgccgacg gggatgccgt gcagcggacc cttgtagtcg cccgccctga
     2581 tctcctgctc ggcgagacgc gccctctcga gcgcggagtc ggcgaagacc gtgatgaagg
     2641 ccttgatctt gctgtcgtag cgggcgatgc ggtcgaggta gagctcgacg agggcgacgg
     2701 gcgacagctc gccggcccgg atgagcgccg cctgctcggt cagggacatg aatgcgaagc
     2761 gctcgtcaag catggccgcc ccccaagtaa cccaccaaca ccggccacgg ctcgttctgc
     2821 aactccccgt acccttcgag gtgggtgagg ccctcgatga gggcgttcac gctggccgtg
     2881 acctccacga gctcctcctc ggggatcacg tagccctgct gcttgagcat gagtctcacc
     2941 tgttcgatgt cgaggtccag atcgccatct cgtgttgttt gtgccatacg gccactcact
     3001 tcctgatcct ggagtcgtaa gcgcgtgccc gacctgccgg tggcttcact tcccgttgac
     3061 gacaggctcg gccgtcgcgc cgttgcaaat tatgtaggcg tagtcgcccg ggttgacccg
     3121 cgagagcgtc ccgcgcagca tgatcaccac ggactggtcg taaggggcgg tcaactcctc
     3181 gagcagttcg aacgtgtgcg gcgagtagac gcgtccgagg accgtgccgg ccggtaccgt
     3241 gtcgcacagc cgatcgaagc cgagttcggg cacgaag
//
LOCUS       DAOQYE010000112         3266 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_30980_length_3266_cov_2.984740, whole genome shotgun sequence.
ACCESSION   DAOQYE010000112 DAOQYE010000000
VERSION     DAOQYE010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 3266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3266
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30980_length_3266_cov_2.984740"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="PKN52_02840"
     CDS             <1..364
                     /locus_tag="PKN52_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98908.1"
                     /translation="LLGDDDIVKHVAGVTPEAAARNLVAAANDRGSPDNATAVVLKAE
                     SVGARQKRYDLPSEPLVSASVDIGETLSGIKAVEEDFPGNGPWHRVKRHPLFPYRYWL
                     MGCAYLLVLLVLFIVWRG"
     gene            376..753
                     /locus_tag="PKN52_02845"
     CDS             376..753
                     /locus_tag="PKN52_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNQ98909.1"
                     /translation="MHEIIATDSAPKAIGPYSQAVKIGGLVFTAGQIALKPDGTWAGG
                     DIAAQTHQVMRNLTAVLEAAGTGVEHIVKSTCFLADMNDFAAFNEVYGSYLKGNLPAR
                     STVQVARLPKDGLVEVEVVAALP"
     gene            complement(886..1584)
                     /locus_tag="PKN52_02850"
     CDS             complement(886..1584)
                     /locus_tag="PKN52_02850"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HNQ98910.1"
                     /translation="MQISMKVRRYDPEKDARPHWQEFNLDVAPTERVLDVLNHIKWEL
                     DGSLTFRRSCAHGICGSDAMMINGMNRLACKILVKDLGDRITVEPLRGLPVLKDLIVD
                     MEPFFDSYRAILPYFINDDPPPPRERLQSVEDRARFDDTTKCILCAACTTSCPVFWTN
                     GRYIGPAAIVNAHRFIFDSRDEGSAQRLRVLNDVNGLWRCRTAYNCTEACPRGIPITK
                     VIEETKRELIWQRV"
     gene            complement(1593..>3266)
                     /gene="sdhA"
                     /locus_tag="PKN52_02855"
     CDS             complement(1593..>3266)
                     /gene="sdhA"
                     /locus_tag="PKN52_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HNQ98911.1"
                     /translation="YAAHNPKVSVAVVTKLYPTRSHTGAAQGGVGAALGNVSEDHPEW
                     HAFDTVKGGDYLTDQDVAEAFSHEVIDAVYELEHMGLPFSRTPEGRIAQRKFGGHTRE
                     HGKAPVERACYAADRTGHMILQTLFQQSIKDRVHFFNEFHVLDIIMDGGECRGVVAYE
                     LATGDLHVIQAKATVLATGGNGRMFKVTSNAHALTGDLTSIVWRRGIPVEDPEFYQFH
                     PTGLYKLGVLLTEGARGEGGILRNADGERFMERYAPTIKDLAPRDMVSRAMYLEVREG
                     RGCGPNKDYIHLDLTHIDAHIIEERLPDITEFARIYLGVDPIKEMVPIQPTAHYMMGG
                     VPTTIDTNVISDGANTIIPGLYAAGEVACASLHGANRLGTNSLGDLLVFGRRAGRNAS
                     AYAETVGRFPDLPAGVLDHTKGLIDAALNGSHKERVSGIRADLQATMMDNASVFRTAE
                     TLATQVEEVGRLRERYKQIGVDDKGKRYNTELLEALELGFLLDNAQQLVTAALNRTES
                     RGAHSREDFKERDDTNWLKHSLVYQDGDGVRIDYKPVTLGRYEPKPRVY"
BASE COUNT          483 a         1041 c         1160 g          582 t
ORIGIN      
        1 gctgctcggc gacgatgaca tagtcaagca cgtcgccggg gtgacacccg aggccgcggc
       61 ccgcaacctc gtggccgccg ccaacgaccg gggcagccct gacaacgcga cggcggtcgt
      121 cctcaaggcg gagtcggtgg gcgcgcggca gaagcgttac gacttaccct cggaaccgct
      181 cgtgtcggcg tccgtagaca tcggtgagac gctctcgggc atcaaggccg tcgaggagga
      241 cttcccgggg aacggcccgt ggcaccgcgt caagcgccac ccgctcttcc cgtaccgtta
      301 ctggctgatg ggctgcgcct acctgctggt cctgctcgtg ttgttcatcg tgtggcgtgg
      361 ttgataggct gcggcatgca cgagatcatc gcaactgatt cggcgcccaa ggccatcggt
      421 ccctactcgc aggctgtcaa gatcggcggg ctggtcttca ccgccggtca gatcgccctc
      481 aagccggacg gcacctgggc gggcggcgac atagcggcgc agacccacca ggtcatgcgc
      541 aacctgacgg ccgtgctgga ggccgccggt accggcgtcg agcacatcgt gaagagcacg
      601 tgcttcctgg ccgacatgaa cgacttcgcc gcgttcaacg aggtctacgg gagctatctg
      661 aagggcaacc tacccgcgcg gagcaccgtg caggtcgcgc ggttgccaaa ggacgggctc
      721 gtcgaggtcg aggtcgtcgc ggccctgcct tgaggccgtc gtcgcggccc cgcactgagg
      781 ccgagggggc cgcggccacc cacggcgagc actctgtcgc ctagcgataa atagaagcgg
      841 cgcccacatg tcgcgcgggc accgctcggt cgttaggtga gccagctata cgcgctgcca
      901 gatcagctcg cgcttcgtct cctcgatcac cttggtgatg gggataccgc gcgggcacgc
      961 ttccgtgcag ttgtaggccg tgcggcagcg ccagagcccg ttcacgtcgt tcaggacgcg
     1021 taggcgttgg gccgagccct cgtcgcgcga gtcgaagatg aagcggtgcg cgttgacgat
     1081 ggccgccggg ccgatgtacc gcccgttcgt ccagaacacg gggcacgacg tcgtgcaggc
     1141 ggcgcagagg atgcacttgg tcgtgtcgtc gaagcgggcc cggtcctcga cgctctgcag
     1201 ccgttcccgc gggggcggcg ggtcgtcgtt gatgaagtac gggaggatgg cgcggtagga
     1261 gtcgaagaac ggctccatgt cgacgatgag gtccttgagg accggcagcc cgcgcagcgg
     1321 ctcgacggtg atgcggtcgc ccaggtcctt gaccaggatc ttgcaggcca ggcggttcat
     1381 gccgttgatc atcatggcgt ccgacccgca gatgccgtgg gcgcagcttc ggcggaaggt
     1441 gagcgagccg tccagttccc acttgatgtg gttcagcacg tccagaacac gttccgtggg
     1501 ggccacgtcg aggttgaact cctgccaatg cggcctggcg tccttctcgg gatcgtagcg
     1561 gcgtaccttc atggagatct gcatggcctg cctcagtaga cccgtggctt cggctcgtag
     1621 cggccgagcg ttacgggctt gtagtcgatg cgtaccccgt cgccgtcctg gtagacgagg
     1681 ctgtgcttga gccagttggt gtcgtcgcgc tccttgaagt cctcgcggga gtgcgcgcca
     1741 cgcgactcgg tgcggttgag cgccgcggtg acgagctgct gggcgttgtc gagcaggaac
     1801 ccgagctcca gggcctccag aagctccgtg ttatagcgct tgcccttgtc gtcgacaccg
     1861 atctgcttgt aacgctcccg gaggcgcccg acctcctcga cctgcgtggc gagcgtctcc
     1921 gccgtgcgga acacggaggc gttgtccatc atcgtggcct gtaggtcggc gcggatgcct
     1981 gagacgcgtt ccttatgaga gccgttcagc gcggcgtcga tcaggccctt cgtgtggtct
     2041 agcacgcccg cgggtaggtc ggggaagcgc cctaccgtct cggcgtaggc gctggcgttg
     2101 cggccggcgc ggcggccgaa cacgagcagg tcgccgagcg agttggtacc taggcgattg
     2161 gcgccgtgca gggaggcgca ggccacctcg ccggccgcgt aaaggccggg gatgatggtg
     2221 ttcgcgccgt cgctgatcac gttcgtgtcg atcgtcgtcg gcacgccgcc catcatgtag
     2281 tgggcggtcg gctggatcgg caccatctcc ttgatggggt cgacgccgag gtagatgcgc
     2341 gcgaactccg tgatgtcggg taggcgctcc tcgatgatgt gggcgtcgat gtgcgtgagg
     2401 tcgaggtgga tgtagtcctt gttcgggccg cagccgcgcc cctcccgcac ttccaggtac
     2461 atggcgcgcg agaccatgtc gcgcggcgcc aggtccttga tggtcggggc gtagcgctcc
     2521 atgaagcgct cgccgtcggc gttgcgcagg atgccgccct cgccgcgagc gccctccgtg
     2581 aggagcacgc cgagcttgta gagccccgtt gggtggaact ggtagaactc cgggtcctcg
     2641 acggggatgc cccgacgcca cacgatgctc gtgaggtcgc cggtgagcgc gtgggcgttg
     2701 gacgtgacct tgaacatgcg cccgttgccg cccgtggcga ggacggtggc tttcgcctgg
     2761 atgacgtgca ggtcgccggt cgccagctca tatgcgacca cgccgcggca ctcgccgccg
     2821 tccatgatga tgtccaggac gtgaaactcg ttgaagaagt ggacccggtc cttgatcgac
     2881 tgctggaaca gcgtctgcag gatcatgtga cccgtgcggt cggccgcgta gcaggcgcgc
     2941 tcgacgggcg ccttgccgtg ctcgcgcgta tgcccgccga acttgcgctg ggcgatgcgc
     3001 ccctccggcg tacggctgaa gggcagaccc atatgctcca gctcgtagac cgcgtcgatg
     3061 acctcgtgcg agaaggcttc cgcgacgtcc tggtcggtca ggtagtcgcc acccttgacc
     3121 gtgtcgaagg cgtgccactc cgggtggtcc tccgagacgt tgccaagcgc ggcgccgacg
     3181 ccgccctggg cggcgcccgt atgcgagcgc gtcgggtaga gcttggtgac gacggcgacg
     3241 gacaccttcg ggttgtgcgc cgcgta
//
LOCUS       DAOQYE010000113         3265 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_30989_length_3265_cov_3.779439, whole genome shotgun sequence.
ACCESSION   DAOQYE010000113 DAOQYE010000000
VERSION     DAOQYE010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 3265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3265
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30989_length_3265_cov_3.779439"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="PKN52_02860"
     CDS             complement(<1..315)
                     /locus_tag="PKN52_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98912.1"
                     /translation="MRRLLAFTVLALLGSLGVAGAQAGLEAGFHVGIILPGPGAEGDA
                     ISAAVARAAAQGAAMANDEFGFNAEMLDYEFSAEAVVADGPEAVVAAARTLLDEHGVY
                     GIV"
     gene            535..1194
                     /locus_tag="PKN52_02865"
     CDS             535..1194
                     /locus_tag="PKN52_02865"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNQ98913.1"
                     /translation="MRILLLEDNRDIAGPLVEALQSERYDVSWVEQPDDVFREFTDSG
                     FDLAILDVMFPGNDDAGFEVATTLRNQGFAAPVMFMSARDTEADAVMGLDLGGDDYLY
                     KPFGLREFMARVRALLRRAADTKRAVFERADLRIDLTTRRVMWRGSNVEVTEREFAML
                     EYFAHYPDRTFGTDELLDRFFPEAASGPSVVRVYVSQLRHKISDGIIQTVPGGYRLGP
                     A"
     gene            1196..2164
                     /locus_tag="PKN52_02870"
     CDS             1196..2164
                     /locus_tag="PKN52_02870"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNQ98914.1"
                     /translation="MRRAVFVAVLGAVVCAIIVEGVLDVWIDQFEDERLFLDLIDIPI
                     MLGIAVLGAWLLARRIAAPLKRLTAATRHVAQQSFDRPVTVPAGKDELAELATSFNTM
                     AATIEGYVERERAFTRYASHELRTPLSAMRLQIERAQLGYVPAAEVMPALARGVAQLE
                     EILAALLALARAGEPHPDTRSATAVVADVIASLDPSDRDRITLLPATEDTPVRHARLF
                     QQAVTNLLDNALRHGSGAATVELRVGERLVTVHVNDEGPGLGATELERVTEPFYRAGD
                     TAGIGLGLSFVAFLAKALEGDLNLSNSERGLVAELSLPIVVGSVAP"
     gene            complement(2297..>3265)
                     /gene="rpoD"
                     /locus_tag="PKN52_02875"
     CDS             complement(2297..>3265)
                     /gene="rpoD"
                     /locus_tag="PKN52_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HNQ98915.1"
                     /translation="EELEARAEAMADARPKTNDPVRQYLQEIGRVKLLTLDEEIDLAR
                     RIEEGEASRGLLQEWTTASGDERERRRHQRIVEDGDLARKHLIEANLRLVVSIAKKYN
                     GRGMSFLDLIQEGNQGLIRAVEKFEYRRRYKFSTYATWWIRQAINRAIADQSRTIRIP
                     VHMVETINKLTRASRRLQQELSREPTFAEIADAMGPDWNAEKVEEAFKLTREPFSLET
                     PIGDEEDSFYGDFIPDDNIESPVDEASKNILSEELDEALNKLNEREAMVLKLRKGLVD
                     GREHTLEEVGSYFGVTRERIRQIENKALRKLKYHESRTRKLRDFLD"
BASE COUNT          474 a         1144 c         1078 g          569 t
ORIGIN      
        1 gacgatgccg tacacgccat gctcgtcaag cagcgtccga gcggcggcca cgacggcctc
       61 cggtccgtct gccacgacgg cctccgctga gaactcgtag tcgagcatct cggcgttgaa
      121 cccgaactcg tcgttcgcca tcgcggctcc ctgcgcggca gcacgcgcga ccgccgcgct
      181 gatggcgtcg ccctcggcgc ccggaccggg caggatgatc ccgacgtgga agccggcctc
      241 caagccggct tgggctccgg ccacccctag tgaaccgagg agggccaaga cggtgaacgc
      301 caggaggcgt ctcatgctcg cccacccctg gcttcatcgt tgccgatcga tattctgacg
      361 gttctactca acttcgttcc gcctcccatc ctgttccccc gcctgcgacg aagcgcgacc
      421 gtcggcggtg cggtcgacgg aatgcttccg attcggggcg atgatatagg atgacgccag
      481 tacacccggt ccgccagtca gcctaagcga agcaggggaa cctcaaggaa cggcatgcgc
      541 atactgctcc tcgaagacaa ccgcgacatc gcaggaccgc tcgtcgaggc gctccagtcg
      601 gagcgctacg acgtcagctg ggtcgagcag cccgacgacg tcttccggga gttcacggac
      661 agcgggttcg acctcgccat cctggacgtc atgttcccgg gcaacgacga cgccggcttc
      721 gaggtcgcca ccacgttgcg caaccagggc ttcgcggcac ccgtgatgtt catgagcgcc
      781 cgcgacacgg aggccgatgc cgtcatgggg cttgacctcg gcggcgacga ctacctctac
      841 aagccgttcg gactgcgcga gttcatggcc agggtgcggg cgctgctgcg ccgcgcggcc
      901 gacaccaagc gcgccgtgtt cgagcgggcc gaccttcgca tcgacctgac cacgcgccgg
      961 gtcatgtggc ggggctcgaa cgtcgaggtc acggaacggg agttcgccat gctcgagtac
     1021 ttcgcccact acccggaccg cactttcggc accgacgaac tcctcgatcg cttcttcccg
     1081 gaggcggcgt ccggcccgag cgtcgtgcgc gtctacgtca gccagctccg ccacaagatc
     1141 tcagacggga tcatccagac cgtgccggga ggataccggc tcgggccggc ctgacgtgcg
     1201 ccgcgccgtc ttcgtcgccg tcctcggggc cgtggtctgc gccatcatcg tggagggcgt
     1261 cctagacgtc tggatcgacc agttcgagga cgagcggctg ttcctggacc tcatcgacat
     1321 cccgatcatg ctcggcatcg ccgtcctggg ggcgtggttg ctggcgcgcc gcatcgccgc
     1381 tcctctgaag cggctcacgg cggccacccg gcacgtcgcc caacagtcct tcgaccgacc
     1441 cgttactgtg ccggccggca aggacgagct ggcggagctg gcgacgagct tcaacaccat
     1501 ggcggcgacc atcgaggggt acgtagagcg ggagcgcgct ttcacgcgct acgcctccca
     1561 cgagttacgc acgccgctga gcgccatgcg gctgcagatc gagcgtgccc agctcggtta
     1621 cgtccccgcg gcggaggtca tgccggcgct cgcgcgcggg gtggcacagc tcgaggagat
     1681 cctggccgcg cttctcgcgc tcgcgcgggc cggggagccg caccccgaca cccgctcggc
     1741 caccgcggtg gtagccgacg tcatcgcctc gctcgacccg tccgaccgcg accgcatcac
     1801 gctcctcccg gccaccgagg acacgcccgt taggcacgcg cgcctcttcc agcaggcggt
     1861 gaccaacctg ctcgacaacg ccctgaggca tgggagcgga gcggcgacgg tggaactgcg
     1921 cgtgggcgag cgcctggtca cggtgcacgt caacgacgag gggccggggc tcggcgccac
     1981 cgaactcgag cgcgtcaccg agccgttcta ccgggccggc gacaccgccg ggatcggtct
     2041 cggcctctcg ttcgtcgcgt tcctggcgaa ggccctcgag ggtgacctca acctctccaa
     2101 ctctgagcgg ggcctagtgg cggagctctc gctcccgatc gtcgtcggct ctgtggcgcc
     2161 ctgacgaagg ctcgggccta cgcgcctcgg cagcgttaga ccgttggatc agccggactc
     2221 ggcccagaaa gacgagtgcg cccctctgga gagccttctc tagaggggcg cactcgcttg
     2281 cctggccggg tcgtgctcag tccaaaaagt ccctgagctt gcgcgtccgg ctctcgtggt
     2341 acttgagctt gcgcagcgcc ttgttctcga tctgccggat gcgctcgcgc gtgacgccga
     2401 agtagctgcc gacctcctcg agcgtgtgct cgcggccgtc gacgaggccc ttgcgcagct
     2461 tgagcaccat cgcctcgcgc tcgttcagct tgtttagggc ttcgtcgagc tcctcgctga
     2521 ggatgttctt cgaggcctcg tccacgggcg actcgatgtt gtcgtccggg atgaagtcgc
     2581 cgtagaagct gtcctcctcg tcgccgatag gggtctcgag ggagaacggc tcgcgcgtga
     2641 gcttgaacgc ctcctcgacc ttctcggcgt tccagtcggg acccatggcg tcggcgatct
     2701 cggcgaaggt cggttcgcgc gagagctcct gctgcaggcg ccggctcgcg cgggtgagct
     2761 tgttgatcgt ctcgaccatg tgcactggga tacggatggt cctggactgg tcggcgatgg
     2821 cgcggttgat ggcctgcctg atccaccacg tggcgtacgt ggagaacttg tagcgacggc
     2881 ggtactcgaa cttctccacg gcccggatga ggccctggtt gccttcctgg atgaggtcga
     2941 ggaagctcat gccgcgcccg ttgtacttct tggcaatgct gaccacgagg cgcaggttgg
     3001 cctcgatgag gtgcttgcgg gcgaggtcgc cgtcctcgac gatcctctgg tggcgccgcc
     3061 gctcgcgctc gtcgcccgac gccgtggtcc actcctgcaa gagcccgcgc gaggcctcgc
     3121 cctcctcgat gcgcctggcg aggtcgatct cctcgtccaa cgtgaggagc ttgacgcgcc
     3181 cgatctcctg caggtactgc ctgactgggt cgttcgtctt cgggcgggcg tcggccatgg
     3241 cctccgcgcg cgcctcgagc tcctc
//
LOCUS       DAOQYE010000114         3260 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_31058_length_3260_cov_3.654290, whole genome shotgun sequence.
ACCESSION   DAOQYE010000114 DAOQYE010000000
VERSION     DAOQYE010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 3260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3260
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31058_length_3260_cov_3.654290"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..793)
                     /locus_tag="PKN52_02880"
     CDS             complement(<1..793)
                     /locus_tag="PKN52_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HNQ98916.1"
                     /translation="MEQRSPGEQRSLKEQRDQRLRNLEALVERGFEAYPYGYAVTHTA
                     ARLHELHPAPEPGARLGEERVVVAGRVLLLRMLGKLTFATLQDGSGARIQVSFQKDKL
                     EAYNALKKVDLGDWLEVAGTLYATKTGELTVDADAFRPLVKSLRPLPDKHHGLTEKEA
                     RYRQRYLDLMVNPEVKRAFELRGKATSYIRRYLDGLGFLEVETPVLQAVPGGAEARPF
                     VTHHNALDYDFHLRISLELYLKRLIVGGFDAVYEIGRNFRNEGISY"
     gene            complement(871..1341)
                     /locus_tag="PKN52_02885"
     CDS             complement(871..1341)
                     /locus_tag="PKN52_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HNQ98917.1"
                     /translation="MAMRVRITPEGYERLKTTLEQEYARLEEATKILQELTGSSDDYD
                     DTGLEDAKAEKARIELRIDELEQQLSRAEVIETKRRGHVDYGNVVTLKNVKSGETFEV
                     QLVSPVEAGVLEGDVPKVSDASPLGKELMGRSKGEKFKVATQAGTDEYHIESIS"
     gene            1562..>3260
                     /locus_tag="PKN52_02890"
     CDS             1562..>3260
                     /locus_tag="PKN52_02890"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HNQ98918.1"
                     /translation="MSLRSFVLVGLALAALALHGRAAAPESSMGVAPLAPYQELYSAR
                     ATGNAAALERLAFESDRYSAYLAAVELSAWPQLSPALRLRALERALELRMPDPLLRAE
                     KLSLDLMHGELAEAAGETAVAVAAYREALPDPTALAAFLRLEQDPYARANALLTAGKN
                     EEALAALGGLSAPSIEAPALRALGRYEEALSAYEAWLAADPGNATAALGRAWCFYYLR
                     RDDAAADAFAALGQAGNYGLGLLANRAGRTDAAVDYLRATGRADYTWLATDYLEAQGR
                     YADAIPIYLELARGGSSYSDDAAYRAYVLAQRIGDAGTERAALDLIPLGSFFALKLGG
                     LPAAPDPTSAPVGEYTRAAILGTASELAAPALTLASYRAGSGDKPAAVGELLFGLRDA
                     EAAGDVYAVLQLGEALQGLDEYRQSVRAARALLASGIDDLRAWRLAYPPAWPATVRAA
                     AMDNDIEPALIWAVMRQESAFSPVAVSRSNAQGLMQVVPTTWDWLAELRKEQPGDPFD
                     VVANIEYGATYLRWLLNYFSGDEELVIAGYNGGQGYVRRTLEGDVIKGDRDDFYRLMD
                     "
BASE COUNT          456 a         1161 c         1103 g          540 t
ORIGIN      
        1 tgtagctgat gccctcgttg cggaagttgc ggccgatctc gtagacggcg tcgaacccgc
       61 cgacgatgag gcgcttcagg tagagctcca gcgagatgcg caggtggaag tcgtagtcga
      121 gcgcgttgtg gtgggtgacg aacggccgcg cctcggcgcc gcccggcacg gcctggagca
      181 cgggcgtctc gacctcgagg aagccgaggc cgtcgaggta gcggcggatg tagctcgtgg
      241 ccttgccgcg cagctcgaag gcgcgcttca cttccgggtt gaccatgagg tcgaggtagc
      301 gctggcggta cctcgcctcc ttctccgtga ggccgtggtg cttgtccggc agcgggcgca
      361 ggctcttcac caggggtctg aacgcgtccg cgtcgacggt cagctcgccc gtcttggtgg
      421 cgtacagcgt gcctgccacc tcgagccagt cgccaaggtc gaccttcttc agcgcgttgt
      481 aggcctcgag cttgtccttc tggaaggaga cctggatgcg cgcgcccgag ccgtcctgca
      541 gggtggcgaa ggtgagcttg ccgagcatgc gcaggagcag gacacgtccg gccacgacga
      601 cgcgctcctc gccgagcctc gcgcccggct cgggggccgg gtgcaactcg tgcaggcgcg
      661 cggccgtgtg ggtgaccgcg tagccgtacg ggtacgcctc gaacccgcgc tcaacgaggg
      721 cttccaggtt gcgcagccgc tggtcgcgct gctccttcag cgagcgttgc tcgccagggg
      781 agcgctgttc cacgtgcgct cccctggcgg cctggcgtgg gtcatgctcc gacccccgat
      841 cagagttgtc ggtcatcgcg ctcgccgtct ctaacttatg gactcgatgt ggtactcgtc
      901 ggtgccggcc tgagtggcga ccttgaactt ctcgcccttc gatcggccca tgagctcctt
      961 gccgagcggg gaggcgtcgg agaccttggg gacgtcgccc tcgaggaccc ccgcctcgac
     1021 cggggagacc agctgaacct cgaaggtctc gccggacttg acgttcttga gggtcacgac
     1081 gttaccgtag tcgacgtggc cacggcgctt ggtctcgatg acctcggcgc ggctgagctg
     1141 ctgctcgagt tcgtcgatgc gcagttcgat gcgcgccttc tccgccttgg cgtcctccag
     1201 gccggtgtcg tcgtagtcat ccgacgagcc cgtgagctcc tgcaagatct tcgtggcctc
     1261 ttcgaggcgg gcgtactcct gctccagcgt ggtcttgagg cgctcgtagc cctcgggcgt
     1321 gatgcgaact ctcatcgcca tgtggttctc cttaagacgc tcactagagt gagctcgctt
     1381 gaatgacttg aactagggac cgttagcaag cggcttgccc taacacgagg acaagccgac
     1441 atgctcccaa ctgtcctgct agtttacccc accccgatga gaagcgggaa acgtggctta
     1501 ccaggcggca aagtcggggt gatttcacct tgcacactcc ccggctgctt acacttggcg
     1561 catgtcgttg cgcagcttcg tcctggtcgg cctggcgctc gccgccctag ccctccacgg
     1621 tcgggccgca gcgcccgagt cttccatggg cgtcgccccg ttggcgccct atcaagagct
     1681 gtactccgcg cgggcgacgg gcaacgccgc cgcgctcgag cgcctcgcgt tcgaaagcga
     1741 tcgctactcc gcctacctcg ccgcggtcga gctctcagca tggccgcagt tgagccccgc
     1801 gctgcgcctc agggcgctcg agcgcgccct ggagctgcgc atgcccgacc cgctgctgcg
     1861 tgcggagaag ctgagcctcg acctcatgca cggcgagttg gccgaggctg caggcgagac
     1921 ggcggtcgcg gtcgcggcct accgggaggc gcttcccgac ccgacggcac tggcggcctt
     1981 cctgcgcctg gagcaggacc cttacgcgcg agccaacgcc ctcctcacgg cgggcaagaa
     2041 cgaggaggcc ctggcggccc tcggcgggct gtccgccccg tcgatcgagg cgccggcact
     2101 acgcgccctc ggccggtacg aggaggctct ctccgcgtac gaggcctggc tcgccgccga
     2161 tcccggcaac gccaccgcag cgctaggacg agcgtggtgt ttctactacc tgcgccgcga
     2221 cgacgccgcg gccgacgcct tcgccgccct agggcaggca ggcaactacg gcctcggcct
     2281 gctggcgaac cgggcaggcc ggacggacgc ggccgtcgac tacttgcgcg ccacgggccg
     2341 ggccgactac acgtggctcg ccaccgacta cctcgaggcg caggggcggt acgccgacgc
     2401 catccccatc tacctggaac tcgcgcgggg cggcagttca tactccgacg acgcggccta
     2461 ccgggcctac gtgctcgcgc agcggatcgg cgacgccggg acagaacgcg ccgcgctcga
     2521 cctgatcccg ctcgggagct tcttcgcact caagctgggc gggctgccgg ccgcgcccga
     2581 cccgacctcg gcgccggtgg gcgagtacac gcgcgccgcc atcctcggca ccgcgtccga
     2641 gctggccgct ccggcgctca cgctggcctc ctaccgggcc ggctccggcg acaagcccgc
     2701 ggccgtcggc gagctcctct tcggcctgcg cgacgcggag gctgccggcg acgtctacgc
     2761 cgtgctccag ctcggcgagg ccctgcaagg gctggacgag taccggcaga gcgtgcgcgc
     2821 cgcccgcgcg ctgctcgcga gtgggatcga cgacctgcgc gcatggcgcc tcgcctaccc
     2881 gcccgcctgg ccggccactg tgagggcggc cgccatggac aacgacatcg agcctgcgct
     2941 catctgggcg gtcatgcggc aggagtcggc gttcagcccc gttgccgtct cgcgctccaa
     3001 cgcgcagggg ctcatgcagg tcgtgcctac cacttgggac tggctagccg agctccgcaa
     3061 ggagcagccc ggcgacccct tcgacgtggt cgcgaacatc gagtacggcg ccacctacct
     3121 ccgctggctg ctcaactact tctccggcga cgaggagctg gtcatagccg gctacaacgg
     3181 cggccagggc tacgtccgcc gtaccctgga aggcgacgtc atcaagggcg accgcgacga
     3241 cttctaccgc ctgatggacc
//
LOCUS       DAOQYE010000115         3243 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_31297_length_3243_cov_3.229925, whole genome shotgun sequence.
ACCESSION   DAOQYE010000115 DAOQYE010000000
VERSION     DAOQYE010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 3243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3243
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31297_length_3243_cov_3.229925"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /locus_tag="PKN52_02895"
     CDS             complement(<1..634)
                     /locus_tag="PKN52_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98919.1"
                     /translation="MSVSSTSHARARGRGGAAAFYALAAAVAFIALPFGRPGRPFLTV
                     DPAFRPWNIDAPWLWLVPLLALVAAVAALAPLETIRRGNILTFAGAGGFVVGAGWLMA
                     TKTPFGMGALLALCLLVVVVGYGLSLSGRVRGDPFVASSILFTGLLVVLLVVYPLITV
                     LQASVVVNGKFTFAKFLSVLQTNRFILIENQFTPRSEGALVRYFALAGLVL"
     gene            complement(758..1765)
                     /locus_tag="PKN52_02900"
     CDS             complement(758..1765)
                     /locus_tag="PKN52_02900"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNQ98920.1"
                     /translation="MKKLFAGVVLSLLIFAGFGNAQRVSVLCSPDLAWCEALGPAFKA
                     ATGIDLDFIRLGSSEALARLRAEAANPVFDVWFGGTGDPHLAAFNEGLTEFYKPSSWD
                     DLLPSLTKSVGETYIPLYTGALGFVVNEGILAEHGVETPQCWADMTNPALKNLIAMPD
                     PNASGTAYTAIATLVQIMGEDAAFDYLAQLHQNVQQYTRSGAAPGQLAGRGDVAIAIQ
                     FMHDGVKFAEEGFPLTVIAPCEGTGFEIGGLSLIKGAPDRDEAIKFIEWALSPEAQMV
                     AATVGSYQVQSNSKTPVPAAAPDLDTIKLIDYDFAKYGDPAERDRLVARWTDEIFPQP
                     R"
     gene            complement(1958..2902)
                     /locus_tag="PKN52_02905"
     CDS             complement(1958..2902)
                     /locus_tag="PKN52_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98921.1"
                     /translation="MIFRHFGRRFMLTVLLLAGAAFSQSPLTGTFAEPTIGFLFVFQD
                     APGGVNGVIVGQDGPIQLNIWPDPQFDGRSARGAFAAGGMNIGFTAQLQPDGVGLAVT
                     LFQLDGAGQPVAGSYEYYMAVREAQTSGSPFPAAPQGAPAGFPTQVPPTQAPFFPPAQ
                     NTPPTQGFPPTQGAPAQGAPAQGAPNQGGAPLQTGPVGATGAPAIVGTWQGIIEQQGV
                     QIIAVTMFNADGTYRDEAYLDGQLMLFYTGTYAFAADGSLTKWITGASPQVCARGNCQ
                     PNQYEPTEFGRIDFLGPDTAVYTEGVGAEATQLRFERR"
     gene            3063..>3243
                     /locus_tag="PKN52_02910"
     CDS             3063..>3243
                     /locus_tag="PKN52_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98922.1"
                     /translation="MDEAIRLPRTTFRVPALVMFGAVLLLAACNKPAPTPPPTPELTA
                     EALDSWWATTPAVTLN"
BASE COUNT          568 a         1033 c         1140 g          502 t
ORIGIN      
        1 cgaggaccag gccggcaagc gcgaagtagc gcaccagagc gccctcgctg cgaggcgtga
       61 actggttctc gatcaggatg aagcggttgg tctgcaggac cgacaagaac ttcgcgaacg
      121 tgaacttgcc gttcacgacg acggacgcct gcagcaccgt gatgagtggg tagacgacga
      181 gcagcacgac tagcaggccc gtgaagagga tgctgctcgc gacgaacggg tcgccccgta
      241 cccgcccgga gaggctgaga ccgtaaccga cgaccacgac gagtaggcag agcgccaaaa
      301 gcgcgcccat gccgaacggg gtcttcgtgg ccatcaacca cccggcgccg acgacgaagc
      361 cgcctgcccc ggcgaaggtc aggatgttcc cgcgcctgat ggtctccaac ggcgccagcg
      421 cggccacggc ggccacgagc gccagaagcg gtacgagcca gagccacggg gcatcgatgt
      481 tccacggccg gaacgccggg tctactgtga ggaacggtct acccggccgt ccgaaaggca
      541 gggcgatgaa cgccacggcg gcggcgaggg cgtaaaaggc cgccgcgccg ccgcgccccc
      601 gagcacgcgc atgtgaggtg gaactgacgg acaaggtctc ctccttggtc gggtgcgacc
      661 actggcctcc gcttggcggc ccagccggct cccctagtga accggcgtcg cacccggtgt
      721 tcaggcgcga cgccgtttgc cgatcaggag accgcgatca gcgcggctgc gggaagatct
      781 cgtcggtcca gcgggcgacc aggcggtcgc gttcggccgg atcgccgtac ttggcgaagt
      841 cgtagtcgat gagcttgatg gtgtcgaggt cgggggcggc ggcaggcacg ggggtcttgc
      901 tgttcgactg cacctggtag gagcccaccg ttgcggcgac catctgggcc tcgggcgaca
      961 gggcccactc gatgaacttg atggcttcgt cgcgatcagg cgcgcccttg atgaggctca
     1021 ggccgccgat ctcgaagccc gtgccctcgc agggggcgat gacggtgagc gggaagcctt
     1081 cttcggcgaa cttgacgccg tcgtgcatga actggatggc gatggcgacg tcgccacgac
     1141 cggccaactg cccgggggcg gcgccagagc gcgtgtactg ctgcacgttc tggtgcaact
     1201 gggcgaggta atcgaaggcc gcgtcctcac ccatgatctg cacgagcgtg gcgatggccg
     1261 tgtaggccgt accggaggcg ttcgggtccg gcatggcgat gaggttcttg agggcggggt
     1321 tcgtcatgtc ggcccagcac tgcggggtct cgacgccgtg ctcggcgagg atgccctcgt
     1381 tgacgacgaa gccgagagcg cccgtgtaga gcgggatgta agtctcgccg accgacttgg
     1441 tcaggctcgg gagcaggtcg tcccatgagg acggcttgta gaactccgtg aggccctcgt
     1501 tgaaggcggc gaggtgcgga tcaccggtgc cgccgaacca gacgtcgaac acggggttgg
     1561 cggcctcggc gcgcaagcgc gcaagcgcct cgctggagcc gagacggatg aagtcgaggt
     1621 ctatgccggt ggcggccttg aaggccggac cgagagcctc gcaccaggcg aggtcggggc
     1681 tgcacagcac gctgacgcgc tgcgcgttgc cgaagccggc gaagatcagg agcgataaca
     1741 ccacgcctgc gaacagtttc ttcacgtact acctccacgt agggccaagc ccaggtacac
     1801 ccaaggggat gggctcgggc cggcaatccc cggccccgtt gaatctcgag tgtctcgcgg
     1861 atgatagcgc ctcggcagtt cgggtgtcaa aaaagcaccg tcaactccgc acgtttggca
     1921 cgctcgcgtc gaagccgcgg tggcaaggcc cgccgggtta cctgcgctcg aagcgcaact
     1981 gggtcgcctc ggccccgact ccctcggtgt agacggccgt gtcgggccca aggaagtcga
     2041 tcctgccgaa ctcggtaggt tcgtactggt tcggctggca gttgccgcgc gcgcatacct
     2101 ggggtgaggc gcccgtgatc cacttggtca gggagccgtc cgccgcgaac gcgtaagttc
     2161 cggtgtagaa gagcatgagc tgaccgtcca ggtaggcctc gtcccggtac gtgccgtcgg
     2221 cgttgaacat ggtgaccgcg attatctgga caccctgctg ctcgatgatc ccttgccagg
     2281 tgccgacgat cgccggtgcg cctgtggctc ccactgggcc tgtctgcagt ggagcgccgc
     2341 cttgattggg cgcgccctgg gcaggtgcgc cttgagcagg cgcgccttga gtaggcggga
     2401 acccctgcgt tggcggcgtg ttctgagcgg gcgggaagaa cggggcctgg gtcgggggca
     2461 cttgggttgg gaaacccgcc ggcgcgccct ggggcgcagc cgggaacgga gagcccgacg
     2521 tctgggcctc gcgcaccgcc atgtagtact cgtagctgcc ggcaacgggt tggcccgcac
     2581 cgtccaactg gaacagcgtc actgccaagc cgaccccatc gggctgaagc tgggcggtga
     2641 agccgatgtt cattccgccg gcggcgaagg cgcctctggc gctccgcccg tcgaactgcg
     2701 gatcgggcca gatgttgagt tggatcgggc cgtcctgacc gacgatcacg ccgttgacgc
     2761 cgccaggagc gtcctgaaag acgaacagga acccgatggt gggttccgcg aacgtcccgg
     2821 tcagcgggga ctgagagaac gccgcccctg ccaggagcag caccgtgagc atgaaccgac
     2881 ggccgaagtg cctgaagatc atcgtaacgc ctccaggagc gagcctagag cgcgcccggt
     2941 taaaagaggc ttaaggctgg cccggccccc ggcgaggcgt gaggtttcac cccatccgct
     3001 gcgacacaga gcctctacca tgcatgcgta ggggttaacc cagttgcagt tggaggtaat
     3061 ccatggacga agccatcagg ctccctcgta ctactttccg cgtaccggcc ctagtcatgt
     3121 tcggcgcagt tctgctcctc gctgcgtgca acaaacccgc tccgacaccg ccaccgacgc
     3181 ccgagctgac ggccgaagca ctcgacagct ggtgggcgac aaccccggcc gtgacgctga
     3241 acg
//
LOCUS       DAOQYE010000116         3237 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_31388_length_3237_cov_2.134821, whole genome shotgun sequence.
ACCESSION   DAOQYE010000116 DAOQYE010000000
VERSION     DAOQYE010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 3237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3237
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31388_length_3237_cov_2.134821"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1354)
                     /gene="nrfD"
                     /locus_tag="PKN52_02915"
     CDS             complement(<1..1354)
                     /gene="nrfD"
                     /locus_tag="PKN52_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HNQ98923.1"
                     /translation="MAVAAGKRKAPFKETNRVIRPGETYGGIDDAVLTPVLAPLAKTP
                     MWWWIGFTISAAVLLVYLVSVVKLITTGIGIFGNNIPVAWGMPIINFVWWIGIGHAGT
                     LISAALLLFRQPWRTTVNRFAEAMTIFAVVCAGLYPILHLGRPWVFYWLVPYPNTFGL
                     WPQFRSPLDWDFFAIGTYFTISAVFWFIGLIPDLATLRDRSKTKVQQLLYGILALGWN
                     GSAKAWQRYQRAYLLLAAVSFPLVLSVHSTIAMDFAAAQLPGWNNTIMPPYFVAGAVF
                     AGFAMVLILAVPLRKAFHLEHLVTLRHLDNASKLMLATGMIVVYGYFIEIFTAWYSGV
                     EFERYMIWNRMFGDHAIFFWALIFCNFVAIQPIWFRQVRQSPWALFFIAIIVSVGMWL
                     ERFVIFVVSLTKDFLPSSWADYVSTVWDWSLFIGSLGLFSTLFFLFIRLLPSIATAET
                     K"
     gene            complement(1354..>3237)
                     /locus_tag="PKN52_02920"
     CDS             complement(1354..>3237)
                     /locus_tag="PKN52_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNQ98924.1"
                     /translation="PPVVHAIGHALNAALGNVGATVSYVRPAAARPAIHREELAELVG
                     LMNDGSVKALVVLGANPVYTAPASLDFAGALAKVPFSAKLGLYHDETADAVTWSVPES
                     HFLETWGDARAFDGTATIRQPLILPFYAGKSEIELLGALLGNPSTAGYDVVRNYWRAN
                     VTGSFDDFWRESVYRGAVAGSSSPAVSVSAAPVAEALPAASNFELKLVLDDSLLDGRF
                     ANNGWLQELPKAFSNLTWDNAAVIAPATAESLGVKTGDVVKLTSGGKDLSAPVWVQPG
                     HAVGAISLALGYGRARAGKVGTGVGVNAYKLLDHTAAGAQAVTVAKTNDSHALVSTQT
                     HHSLEGTGEARHIVRSGTLSEFRAAPDHPHFVHPVAHHEADLYPDFEYKGYRWGMVID
                     QTVCTGCNACVVACQSENNIPIVGKAEVARGREMHWIRVDNYYSGSLDDPQFFHQPMP
                     CQQCEKAPCEPVCPVGATVHDSEGLNVMVYNRCVGTRYCSNNCPYKVRRFNWLQYAEL
                     ATDATALSLANNPDVTVRSRGVMEKCTYCTQRIATARIDASVEDRKIRDGEVVTACQA
                     ACPTQAIVFGDLNDPTSQVTAVKASPLNYWMLEELQTFPRTTYLAKVKNPNPALASAE
                     GSH"
BASE COUNT          535 a          985 c         1206 g          511 t
ORIGIN      
        1 ccttcgtctc ggccgtggcg atggacggca gcagtcggat gaagaggaag aacagggtgg
       61 agaagaggcc gaggctaccg atgaacagcg accagtccca gacggtcgac acgtagtctg
      121 cccacgacga gggcaggaag tccttggtca acgagaccac gaagatcacg aaccgctcga
      181 gccacatgcc cacgctgacg atgatggcga tgaagaagag cgcccacggg ctctggcgca
      241 cctggcggaa ccagatgggc tggatggcca cgaagttgca gaagatcagc gcccagaaga
      301 agatggcgtg gtccccgaac atgcggttcc agatcatgta gcgctcgaac tcgacgcccg
      361 agtaccaggc cgtgaagatc tcgatgaagt agccgtacac gacgatcatg cccgtggcga
      421 gcatgagctt cgaggcgttg tccaggtgcc gcaacgtgac gaggtgctcg aggtggaacg
      481 ccttgcgtag gggcaccgcg aggatgagca ccatcgcgaa gccggcgaac acggcgccgg
      541 ccacgaagta gggcggcatg atcgtgttgt tccagcccgg cagctgcgcc gcggcgaagt
      601 ccatggcgat cgtgctatgc acgctgagca cgagcgggaa gctgacggcg gccaggagca
      661 ggtacgcccg ctggtagcgc tgccacgcct tggccgagcc gttccagccc aacgcgagga
      721 tgccgtagag gagttgctgg accttcgtct tcgaacggtc gcgcagcgtc gcgaggtcgg
      781 ggatgaggcc gatgaaccag aagacggccg agatcgtgaa gtacgtgccg atggcgaaga
      841 agtcccagtc gagcggactg cggaactgcg gccagagtcc gaacgtgttg gggtaaggca
      901 cgagccagta gaagacccag ggccggccca ggtggaggat ggggtagagg ccggcgcaga
      961 ccacggcgaa gatcgtcatg gcctcggcga agcggttgac cgtcgtgcgc cacggctgcc
     1021 tgaagaggag cagcgcggcc gagatgagcg tcccggcgtg gccgatgccg atccaccaca
     1081 cgaagttgat gatgggcatc ccccaggcga cggggatgtt gttgccgaag atcccgatgc
     1141 ccgtggtgat cagcttgacg accgacacga ggtagacgag gagcacggcg gccgagatgg
     1201 tgaagccgat ccaccaccac atcggcgtct tggcaagggg agccaggacg ggcgtgagca
     1261 cggcgtcgtc gatgccgccg tacgtctcgc ccggtcggat gacgcggttc gtctccttga
     1321 agggggcctt gcgcttgccg gcagctaccg ccatcagtgg ctcccttccg cgcttgccag
     1381 cgcagggttc gggttcttga ccttggcgag gtaggtggtg cgtgggaacg tctgcaactc
     1441 ctcgagcatc cagtagttga gcggcgaggc cttcacagca gtcacctgcg aggtcgggtc
     1501 gttgaggtcg ccgaagacga tggcctgcgt cggacaggcg gcctggcagg cggtgacgac
     1561 ttcgccgtcg cggatcttgc ggtcctcgac agaggcgtcg atgcgcgcgg tcgcgatgcg
     1621 ctgcgtgcag tacgtgcact tctccatgac gccgcgcgag cggacggtga cgtcggggtt
     1681 attggccagc gacagcgcgg tggcgtcggt ggccagctcg gcgtactgga gccagttgaa
     1741 gcggcgcacc ttgtaggggc agttgttcga gcagtagcgc gtgccgacgc accggttgta
     1801 gaccatcacg ttgaggcctt cggagtcgtg caccgttgcg cctactgggc agacgggctc
     1861 gcacggggcc ttctcgcact gctggcacgg catcggctgg tggaagaact gcgggtcgtc
     1921 gaggctgccg gagtagtagt tgtcgacgcg gatccagtgc atctcgcggc cgcgcgcgac
     1981 ctccgccttc ccgacgatag ggatgttgtt ctccgactgg caggcgacca cgcaggcgtt
     2041 gcagcccgtg cagaccgtct ggtcgatgac catgccccac cggtagccct tgtactcgaa
     2101 gtcggggtag aggtccgctt cgtggtgcgc gaccgggtgg acgaagtgcg ggtggtccgg
     2161 cgccgcgcgg aactcggaca gcgtgccgga gcgcacgatg tggcgcgcct cgcccgtgcc
     2221 ttcgaggctg tggtgcgtct gcgtgctgac gagcgcgtgc gagtcgttgg tcttcgcgac
     2281 cgtgaccgcc tgggctcccg cggccgtgtg gtccaggagc ttgtaggcgt tgacgccgac
     2341 ccccgtgccg accttgcccg cgcgcgcgcg gccgtagccg agcgccaggc tgatcgcgcc
     2401 gaccgcgtgg ccgggctgca cccagacggg ggcgctcagg tccttgccgc cgctcgtgag
     2461 ctttacgacg tccccggtct tgaccccgag gctctcggcc gtcgccggag cgatgaccgc
     2521 ggcgttgtcc cacgtcaggt tgctgaacgc cttcggcagc tcctggagcc agccgttgtt
     2581 ggcgaagcgg ccgtcgagca gcgagtcgtc gagcacgagc ttcagttcga agttcgaagc
     2641 ggcggggagg gcctcggcga ccggcgcggc gctgacgctc acggccggcg agctgctgcc
     2701 ggcgacggcg ccacggtaga cgctctcgcg ccagaagtcg tcgaaggagc ccgtgacgtt
     2761 cgcgcgccag tagttgcgca ccacgtcgta gccggccgtg ctggggttgc cgagcagcgc
     2821 gccgaggagc tcgatctcgc tcttgccggc gtagaagggc aggatgagcg gctgcctgat
     2881 ggtcgccgtg ccgtcgaacg cgcgcgcgtc gccccacgtc tcgaggaagt gcgactccgg
     2941 cacgctccac gtgaccgcgt cggcggtctc gtcgtggtag aggccgagct tggccgagaa
     3001 cgggaccttc gcgagggcgc cggcgaagtc gaggctcgcc ggggcggtgt agacggggtt
     3061 cgcgccgagg acgacgagcg ccttgacgga gccgtcgttc atgaggccga cgagctcggc
     3121 gagctcctcg cggtggatgg ccggtctggc cgcggcgggt cgcacgtagc tgacggtggc
     3181 gccgacgttg ccgagcgcgg cgttgagcgc gtggccgatg gcgtgcacga cgggcgg
//
LOCUS       DAOQYE010000117         3220 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_31612_length_3220_cov_3.698262, whole genome shotgun sequence.
ACCESSION   DAOQYE010000117 DAOQYE010000000
VERSION     DAOQYE010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 3220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3220
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31612_length_3220_cov_3.698262"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(187..1488)
                     /locus_tag="PKN52_02925"
     CDS             complement(187..1488)
                     /locus_tag="PKN52_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNQ98925.1"
                     /translation="MKRLCFILACIALLSTVYAQTKIDFWYAFSDANRSGWIQERMDE
                     FNAQLSAAGKPYMIVGERKGSYPETQQAAVLAARQGNPPHLVQLYEVGSQIAVDSGIF
                     MPVIELGDFDTSDYIAPVLNYYTIGGKVNSIPFNSSSPIVYANKSLMDAAGVAAVPTT
                     YNELLAACEAATAAGVLTADVKCFGVSLNGWFVEQWVAEQGATLVNNDNGRSGRATEV
                     LLTSDAVRNVFNWFKTLNDKGYYTYTGKLEDWDGSDAIFTNGKVLFHITSTADLVNIT
                     RAVGTSFELATGVLPIVDGTERNGVVIGGASIWLVRDHPVEELAVARDFALYMTNAEN
                     MVSWHKATGYYPVRNSSVDLLRAEGWFTEFPNASTAFDQLLGTTPSTATGGALMGTFQ
                     ATRTIVEEALQKVLAGASVDDALAEAKKLADAALAEYNANF"
     gene            1868..2311
                     /locus_tag="PKN52_02930"
     CDS             1868..2311
                     /locus_tag="PKN52_02930"
                     /inference="COORDINATES: protein motif:HMM:TIGR03562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family peroxiredoxin"
                     /protein_id="HNQ98926.1"
                     /translation="MSIADRTTQTTWLGSLAKGSGKLSKSSSAALDGLGLTWASRTEA
                     PGGKTSPEELLAAAHSSCFSMALSLKLGEHKLEAERLEVTATVSLSEVNGLPTIDRSS
                     LTVRGKVPGASAQQFASIVDEAGKLCPVSRLFAGAKISVEATLES"
     gene            complement(2349..>3220)
                     /locus_tag="PKN52_02935"
     CDS             complement(2349..>3220)
                     /locus_tag="PKN52_02935"
                     /inference="COORDINATES: protein motif:HMM:NF013292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HNQ98927.1"
                     /translation="TGGAGKISPEDRAPYLAGLHRARDAGYQVLADGGTAIAAILAAV
                     TTMEDDLDAFNAGTGGAPNRAGTVECDAAIMSADGTSGAVAAVTRAKNPVLLADKVRL
                     ASPHALIVGPGADALVEDPIDNARLLTPRMRVALEWWRENQQSEPKGSNTVGAVALDD
                     RGRLAAATSTGGMLGKWPGRVGDAPLIGAGTYAAHHVGISCTGDGEAFIRAVTSKGLA
                     DRLLAGVELGAAVQSALDEVAVQNATGGVIVLTADGHIASGFNARDMAYAWRTPAGAD
                     AGVSAEPGVRLLG"
BASE COUNT          536 a         1017 c         1106 g          561 t
ORIGIN      
        1 ctttcctcca ctggccggcc gtttgcgcga gtgcggcttg gggacgcgaa cccggtgcac
       61 ctaacaaatg tgcctgcggc ggcggtatac gagcgagtcg ctaccagccc gcgctgaatc
      121 cgtggggccg ccgcgcatga gatcgcgcgg cggcccgggg gtagtcaggt tagcccggtc
      181 agtacgttag aagttggcgt tgtactcggc tagggcggcg tcggccagct tcttggcttc
      241 cgctagagcg tcgtcgaccg aggcacccgc caagaccttc tgcagtgcct cctcgacgat
      301 ggtgcgggtg gcctggaacg tgcccatcag ggcgccgccg gtcgcagtgc taggggtggt
      361 gccgaggagt tgatcgaacg ccgtgctggc gttcgggaac tccgtgaacc agccctcggc
      421 acggagaagg tcaacactgc tgttgcgcac cgggtagtag cccgtggctt tgtgccacga
      481 caccatgttc tcggcgttgg tcatgtacag ggcgaagtcg cgcgctacgg cgagttcctc
      541 gaccggatgg tcgcgaacga gccagatgct ggcgcccccg atgacgacgc cgttgcgctc
      601 cgtgccgtcc acgataggca gcacaccggt cgctaattcg aaggaggtcc ctacggcccg
      661 tgtgatgttc acgagatcgg ccgtggaggt gatgtggaac aacaccttgc cgttggtgaa
      721 gatggcgtcg gagccgtccc agtcctcgag cttgcccgtg tatgtgtagt aacccttgtc
      781 gttcagggtc ttgaaccagt tgaagacgtt cctcactgcg tcggacgtga ggagaacctc
      841 ggtagcgcgg ccggagcggc cgttgtcgtt gttcacaagc gtagcgccct gctccgcgac
      901 ccactgctcc acgaaccaac cgttgaggct cacgccgaag cacttgacgt cggctgtgag
      961 gacgcctgcg gcagttgcgg cttcgcaggc ggcaagcagt tcgttgtaag tcgtcggcac
     1021 agcggccacg ccggcagcgt ccataaggga cttgttggcg tagacgatgg ggctcgagga
     1081 gttgaagggg atcgagttga ccttaccgcc gatggtgtag tagttaagca ccggggcgat
     1141 gtagtcggac gtgtcgaagt cgccgagttc gatgactggc atgaagatcc ccgagtcgac
     1201 agcgatctga ctgccgacct cgtacagctg aacgaggtgg ggtgggttgc cctgcctggc
     1261 ggcgaggacc gcagcctgct gagtctctgg gtagctgcct ttgcgctcgc cgacgatcat
     1321 gtaaggcttg ccggccgctg agagttgcgc gttgaactcg tccatgcgtt cctggatcca
     1381 accggaacgg ttggcgtcgc tgaacgcgta ccagaagtcg atcttcgtct gtgcgtagac
     1441 ggtcgagagc agtgcgatgc aggccaagat gaagcacaag cgtttcattc ctacctccgt
     1501 ggctatgtgt gtggctacac gtaggacgga tcgcgtgttg atcgcgtagc ctgcccggat
     1561 atccgactgg gcacccaggc atgtcggcag gttatggagc gaacgtcacg gcttcgtcag
     1621 taccgcgtgg ggctgggtac gagctcgagg atagtcagac cggagggcta ctcttcgctg
     1681 gacggagacg gcggtgggga ggccgggcgg tctcaccctc ccgccccact ccgttcttac
     1741 gaacatgtgc gcatccgcac aagcaaagta gggtgaggag acgtggtggg atacgggttc
     1801 gaggccctcg tctcgaggcc ccctcttcga aacctcgacg catgagacca cccaaggagg
     1861 agacgctatg agcatcgccg acaggaccac tcaaacgaca tggctcggct cgctggccaa
     1921 gggctccggc aagctgagca agagcagcag cgcagcgctc gacggtctag gcctcacctg
     1981 ggcatcgcgc accgaggcgc ccggcggcaa gacgagcccg gaggagctgc tcgcggccgc
     2041 ccactcgtcc tgcttctcca tggcgctgtc gctcaagctc ggtgagcaca agctggaggc
     2101 ggagcggctc gaggtcacgg ccacggtgtc gctcagcgag gtgaacgggc ttcccaccat
     2161 cgaccgctcg agcctaacgg tgcgcggcaa ggttcccggc gcctccgccc agcagttcgc
     2221 gtccatcgtc gacgaggcgg ggaagctgtg ccctgtctcc aggctgttcg ccggcgcgaa
     2281 aatcagcgta gaggcgacgc tggagagtta agcggcgagc gacggcgcgt cgagaagaac
     2341 gcgctcggtc aaccgagcaa gcgcacgccc ggctctgcag agacgcccgc atcggccccg
     2401 gcgggagtgc gccacgcgta ggccatgtcg cgagcgttga aaccgctggc gatatggccg
     2461 tccgccgtca ggacgatcac gccgccagtg gcgttctgga cggcaacctc gtcgagggca
     2521 gactgcaccg ccgcgcccag ttcgacgccg gccagaagcc gatcggcgag ccctttcgac
     2581 gtcacggccc gtatgaacgc ttcgccatcg cccgtgcacg agatgccgac gtggtgcgcg
     2641 gcgtacgtgc cggcgccgat gagcggagcg tcgccgaccc ggccgggcca cttgcctagc
     2701 atgccgcccg tgctcgtcgc ggcggcgagc cggccccgat catcaagtgc cacggcgccg
     2761 acagtattgg aacccttggg ttctgactgc tggttctcgc gccaccactc cagcgccacg
     2821 cgcatgcgtg gggtcagcaa ccgggcgttg tctatcgggt cttcgaccag tgcatctgct
     2881 ccaggtccga cgatcaaagc gtgcggcgac gcgaggcgca ccttgtcggc caacaggacg
     2941 gggttcttcg cgcgcgtgac ggccgcgacc gcgccgctgg tcccgtcggc gctcatgatg
     3001 gcagcgtcgc actccacggt ccccgcacgg ttgggcgctc cgcccgtgcc ggcgttgaac
     3061 gcgtccaagt cgtcctccat cgtggtcacg gccgctagga tcgcggcgat ggccgtgccg
     3121 ccgtcggcga ggacctggta accggcatcg cgggcccggt gaaggcccgc caggtagggc
     3181 gctcggtcct caggggagat cttgcccgcg ccgccggtgc
//
LOCUS       DAOQYE010000118         3205 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_31844_length_3205_cov_2.485079, whole genome shotgun sequence.
ACCESSION   DAOQYE010000118 DAOQYE010000000
VERSION     DAOQYE010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 3205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3205
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31844_length_3205_cov_2.485079"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..816)
                     /locus_tag="PKN52_02940"
     CDS             complement(<1..816)
                     /locus_tag="PKN52_02940"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ98928.1"
                     /translation="MFAYVLKRSLSAVVVVVLVATLTFLLIQAAPGGLSILMDPNMSS
                     AEVARLKHNLGLDQPIHIQYFNWLRNAVQGDLGASLSYGGRPVTSMIMERVPATLKLG
                     AASLVLTLVIGIPAGVVAGRRPNGLTDQVISFLSFVALAVPSFWLGIILMILFGAELR
                     WLPTSGMGPRGDLVLGLKHLIMPAVVLASSSAAAVIRYTRSSWLEVASLDYVRTGRSK
                     GLTEGRLGRRHILRNALIPVVTVIGVQLPRLVGGAAVIETLFSWPGLGQLGVDA"
     gene            complement(923..2524)
                     /locus_tag="PKN52_02945"
     CDS             complement(923..2524)
                     /locus_tag="PKN52_02945"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNQ98929.1"
                     /translation="MSRLNTHYRWIVILALAAMVGTTPTALAQGGSGSVRIALQAMPE
                     GFNPVLPAELNANIVTGAMFAPLAAVNPTTFATEPYLAESWEVSDDLMTWTFRLRENA
                     KWHDGVPITADDVKFAFDKIKDPAESASAYVAATNFASIDVIDEHTVQIVLTAPNALL
                     PDTLSSGGFEPLPKHVYEGFAHLVDAVDANTRNPVGSGAFRMANVQPGAQIELVAFDD
                     FFFGKPKVPGLTFKIVRDQNAAVAQIRAGELDYVSIEAVHRQAVAADPRMEIFSVAGT
                     RYVMMAINMSDYEPWHTMFADVRVRKAMMYAVDRVEIADHIGQGLMPVLDGLMPTTLT
                     WIPEPDIVPYRYNPELAMQLLDEAGWVDTNGNGIRDKDGMELSFYILVDRGNAVREQI
                     TPVLQAAWQAIGMDVEYFATERTGRWLEETRDGTFPTRVSTFPIPNADWAYRLFHSKG
                     LNNSQHYVNPEVDNILDTMLASPDIEVRGQLLKQLQEVLNEDPYIMPFFLEPTLHAID
                     KRLQNVPHSELQRAMPYTYLISWAQ"
     gene            2829..>3205
                     /locus_tag="PKN52_02950"
     CDS             2829..>3205
                     /locus_tag="PKN52_02950"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNQ98930.1"
                     /translation="MSDADALHQLRIDPGGDLPIYLQLKYQLSYLITTEKLPPASRLP
                     AVRNLAHQLGVNHHTVAQAYRALQTDGLIDSTVGRGSFVRSFSDLDRVNAARHELLND
                     ALEQARNRARALGFSDQEMIQRLT"
BASE COUNT          576 a          999 c         1052 g          578 t
ORIGIN      
        1 ggcgtcgaca cccagctgac cgagacccgg ccagctgaac aacgtctcga tgaccgcagc
       61 cccgcccacc agcctcggca actgcacccc gatgacggtt accaccggga tgagcgcgtt
      121 cctgaggatg tgcctccggc ccagccgtcc ttccgtcaaa cccttgctgc ggccggtgcg
      181 gacgtagtcc aggctggcga cctccagcca gctggagcgg gtgtagcgga tgacggccgc
      241 agcgctggac gaggcaagca ctactgcggg cattatcagg tgcttgagtc cgaggacgag
      301 gtcaccacgc ggccccatgc cggaggtcgg gagccagcgg agctcggcgc cgaacaggat
      361 catcaggatg atgcccaacc agaaactcgg cacggccagc gcgacgaatg acaggaaact
      421 gatgacctgg tcggtcaggc cgttcggccg ccgcccggct acgacacccg ccggtatgcc
      481 tatgacgagc gtcagcacca gggacgccgc tccgagcttc aacgtggccg gcacgcgctc
      541 catgatcatg gaggtgacgg gccgccctcc gtagctgagg gacgccccca agtcgccttg
      601 gacggcgttg cgtagccagt tgaagtactg gatgtgaatc ggctgatcaa ggccaaggtt
      661 gtgcttcagc cgcgccactt cggctgacga catgttcggg tccatcagta tcgagagccc
      721 gcccggggcc gcctggatca acaggaaggt gagcgtcgcg acgagcacga ccaccaccac
      781 ggcagacagg gacctcttga gaacgtaggc gaacatcgag gcttagtggc gacccatctg
      841 cggctggtgg ggtcgcggtt cgcgacccca ccagcgctgc atcgtctctg tgcgacgtct
      901 agggatcaga cgaccctttc ggtcactgcg cccagctgat gaggtaggtg tacggcatgg
      961 cgcgctggag ttcgctgtgc ggaacgttct gcaggcgctt gtcgatagcg tgcagcgttg
     1021 gctcgaggaa gaacggcatg atgtacgggt cctcgttcag cacttcctgc agctgcttga
     1081 ggagctgacc acgcacttcg atgtcggggc tggccaacat cgtgtcgagg atgttgtcga
     1141 cctccgggtt gacatagtgc tggctgttgt tcaggccctt gctgtggaac agccggtagg
     1201 cccagtcggc attggggatg gggaacgtgg agacgcgcgt cgggaacgtg ccgtcccttg
     1261 tctcttccaa ccatctgccc gtacgctcgg tagcgaagta ctcgacgtcc atgccgatag
     1321 cttgccaagc ggcctgcagc accggcgtga tctgctcgcg gacggcatta cccctgtcga
     1381 cgaggatgta gaagctcagc tccatgccgt ccttgtcgcg gatcccgttg ccattggtat
     1441 cgacccaacc agcctcgtcc agcaactgca tggcgagctc ggggttgtag cggtagggga
     1501 cgatgtcggg ctccggtatc cacgtgaggg tcgtcggcat gaggccgtcc agcaccggca
     1561 tcaggccctg cccgatatgg tcggcgatct ccacgcggtc gaccgcgtac atcatggcct
     1621 tacggacgcg gacgtcggcg aacatggtgt gccacggctc gtagtccgac atgttgatgg
     1681 ccatcatcac gtaccgggtg ccggcgacgg agaagatctc catgcgcgga tcggcagcca
     1741 cggcttgcct gtgaaccgcc tcgatggaca cgtagtcgag ctcgccggcg cggatctggg
     1801 cgacggcggc gttctggtcg cgcacgatct tgaaggtgag ccccggaacc ttcggcttcc
     1861 cgaagaagaa gtcgtcgaag gccacgagct cgatctgcgc acccggctgt acgttggcca
     1921 tgcggaacgc cccgctgccg acagggttgc gcgtgttcgc gtcgaccgcg tccaccaaat
     1981 gggcgaagcc ctcgtagacg tgctttggca gcggttcgaa cccgccggag ctgagggtgt
     2041 cggggagcag ggcgttcgga gcggtgagga cgatctggac cgtgtgttcg tcgatgacgt
     2101 cgatcgacgc gaagttggtg gcagcgacgt aagcgctcgc cgactctgcc ggatccttga
     2161 tcttgtcgaa ggcgaacttc acgtcgtccg ccgtgatagg tacgccgtcg tgccacttcg
     2221 cgttctcgcg caggcggaac gtccaggtca tcaggtcgtc ggagacctcc cagctctcgg
     2281 ccaggtaggg ctcggtcgcg aaagtcgtcg ggttgacggc ggccagcggt gcgaacatgg
     2341 cgcccgtcac gatgttggcg ttcaactcgg ccggcaacac cgggttgaac ccctcaggca
     2401 tcgcctggag cgcgatcctg acgctcccgg acccgccttg agcgagggcc gtgggagtgg
     2461 tgccgaccat ggcggcaagg gcgagtatca ctatccagcg gtagtgggtg ttcaaacgcg
     2521 acacatgctc ctccaaagtg gtgtgagcga cgttacggtt ggcctttgcg gtcgtcgagc
     2581 gaacctctgc gactttgacc cttccctaga attgtggtag tagactaggg cattgtgata
     2641 acggtgtcaa cacgcccgcc cataccgtgc gacggcaccg agccccgcgt cgaggggccc
     2701 ttctcgcacc gctcaccaag ccagttggtg ttcgtgatgc gaaaacgtat cctagggcaa
     2761 acacgcgtga ccttctttcg catctggagg gagctgctag gcagctatta ggtgggtgac
     2821 ggcatctcgt gagcgacgct gacgcgttac atcagctgag gatcgacccc ggaggcgacc
     2881 tcccgatcta ccttcagctg aaataccaac tgagctacct gataacgacg gagaagctgc
     2941 cgccggcgtc gcgtttgccg gcagtgagga acctcgccca ccaactgggg gtaaatcacc
     3001 atacggtcgc ccaggcctac cgcgcgctgc agacggatgg gctcatcgac tcgaccgtcg
     3061 gtcgcggctc cttcgtccgg agtttcagcg acctggaccg tgtgaacgcg gctcgccacg
     3121 agctgctgaa cgacgccttg gagcaggcgc gcaaccgagc gcgcgcgctc ggcttctccg
     3181 accaggagat gatccagcgc ctcac
//
LOCUS       DAOQYE010000119         3195 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_31982_length_3195_cov_2.811146, whole genome shotgun sequence.
ACCESSION   DAOQYE010000119 DAOQYE010000000
VERSION     DAOQYE010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 3195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3195
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31982_length_3195_cov_2.811146"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(168..791)
                     /locus_tag="PKN52_02955"
     CDS             complement(168..791)
                     /locus_tag="PKN52_02955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98931.1"
                     /translation="MARTAPAKGQGSSLSIKPLGVETWPAFADLIERHNGVWGGCWCV
                     AFHTKSYRDPLLSHRDPKELLVRTDRAHAALVYDGTECVGWCQFGPRNELEAINRKRA
                     YDKETAELPDWRITCFFVDKRYRHRGVARVALRGAVAEIAGLGGGLVESFPEDVTNRK
                     TSSSFLYNGTTAMFEEQGFERGRQIGMHHWVMTRRVPATARAGARER"
     gene            complement(802..1788)
                     /locus_tag="PKN52_02960"
     CDS             complement(802..1788)
                     /locus_tag="PKN52_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNQ98932.1"
                     /translation="MSDQPVEKVRWGFIGAGDVTEVKSGPAFARVPGSSVSVVMRRDA
                     EKARDYAARHGVPRWTTDANAVVEADDVDAVYVATPPSSHAEYVKLAAEAGKPVYVEK
                     PMARTAAEGEEMLATCRAAGVPLYVAYYRRALPRFEFVRDLIQGGSLGTPTMVSLALA
                     RVAPVGEARSAWRWDREVAGAGLLLDLGSHALDLLDHWLGPVAEARGWSATRQPWSNV
                     PDQVVASMRFANGVLGTAAWDFAAAEARDALTVTLTEGAIEVPVFDEGPVRIERGAGG
                     ALERVIPHPPHVQEPLIRTVVADILGAAGACQSTGESALRTQRVMDALLSGR"
     gene            complement(1847..2485)
                     /locus_tag="PKN52_02965"
     CDS             complement(1847..2485)
                     /locus_tag="PKN52_02965"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HNQ98933.1"
                     /translation="MTKTLDPAVRQALDSPTTLMGKLREIAKRIGTDQELAERLWQEA
                     NVNSKLVAILIMDRKSLTPDYLERLLVDIETLDRAEQGKVGEWLLANQLMAAKKLHPG
                     MDGWLQSRSLMRQRLFWSFKARLMRQQKSSTAEAERLLRLIERDLGGADQDLQWVMNY
                     CAAMIGIYDPERRQRCLDLGERLGLYLDYPVPKGCTSPYLPEWIGSQVAKAS"
     gene            2513..>3195
                     /locus_tag="PKN52_02970"
     CDS             2513..>3195
                     /locus_tag="PKN52_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNQ98934.1"
                     /translation="MTTRVRGTTGRVALVTGGASGIGAACAERLLADGLDVVIADRDA
                     QAAGELASRLGCRAVVADLAKREGCADAVAETLELAGALDVLVANAGYQHIEALSTFP
                     EDKWDDMLALMLTGPFLLTKYAWEALTRSGNGRLIYMGSAHSLTASPFKGAYVTAKHG
                     LVGLARVAALEGGQHGITANVVAPAYVRTPLVAGQIADQARTRGISPEEVESKVFLEN
                     TAIKRLLEPA"
BASE COUNT          468 a         1126 c         1095 g          506 t
ORIGIN      
        1 cgtccaggtg gtctttccga tgcgggccgt caccgggatc atgccccacc cgtacgtgac
       61 gtaggccgcc atggccttga tggtcgcgca gtgctcctcc gggacggtaa cgaagtggaa
      121 gggcgccggt ccgcgccagt accagacctc gccggcgaac tcgaacgtca tcgctcgcgc
      181 gcccctgcgc gcgccgtggc gggcacgcgc ctggtcatga cccaatgatg catgccgatc
      241 tgcctgcccc gctcgaaccc ctgctcctcg aacatggcgg tcgtgccgtt gtagaggaag
      301 gagctggagg tcttccggtt agtcacgtcc tcggggaagc tctcgacgag gccgccgccg
      361 agcccggcga tctccgccac ggcgccgcga agggcgaccc tagctacgcc ccggtggcgg
      421 tagcgcttgt cgacgaagaa gcacgtgatg cgccaatcgg gaagctcagc ggtctccttg
      481 tcgtaggcgc gcttgcggtt gatggcctcg agctcgttcc tcggcccgaa ctggcaccag
      541 ccgacgcact cggtgccgtc gtacacgagg gccgcgtgag ccctgtcggt tcgcaccagc
      601 agctctttcg ggtcccggtg gctcaagagg gggtcgcggt agctcttggt gtggaaggcg
      661 acgcaccaac agccgcccca gacgccgttg tggcgctcga tgaggtcggc gaacgccggc
      721 cacgtctcga caccgagcgg cttgatggag aggctcgacc cttgcccctt ggctggtgcg
      781 gtccgcgcca cggccttcga cctaccttcc actgagcagg gcgtccatga cccgctgcgt
      841 cctgagagcg ctctcgcccg tgctctggca agcgcccgcc gcgccaagga tgtcggcgac
      901 cacggtcctg atgagcggct cctggacgtg cggggggtgg gggatgacgc gctccagggc
      961 gccgccggcg ccgcgctcga ttcgcactgg tccctcgtcg aagacgggca cctcgatcgc
     1021 cccttccgtc aacgtgacgg tcagggcgtc gcgggcctcg gccgccgcga agtcccaggc
     1081 cgccgtgccg agcacgccgt tggcgaagcg catgctcgcc actacctggt ccggcacgtt
     1141 cgaccacggt tggcgcgtcg cgctccagcc ccgcgcctcg gcgaccggcc cgagccaatg
     1201 atcgaggagg tcgagggcgt ggctgccgag gtcaaggagc aggccggcgc cggcgacctc
     1261 gcgatcccag cgccaggccg accgcgcctc accgaccgga gcgacccgcg ccaaggcgag
     1321 gctcaccatg gtcggcgtgc cgaggctgcc gccctggata aggtcgcgca cgaactcgaa
     1381 gcgcggcaag gccctgcggt agtacgccac gtagagggga acgccggccg cccggcaggt
     1441 cgccagcatc tcctcgccct cggccgcagt tcgcgccatg ggcttctcga cgtagacggg
     1501 cttgccggcc tcggccgcga gcttcacgta ctccgcgtgg ctggagggcg gggtggccac
     1561 gtagacggcg tcaacatcgt ccgcttccac cacggcgttg gcgtccgtcg tccaccgggg
     1621 cacgccgtgg cgcgccgcgt agtcgcgggc cttctcggcg tcacggcgca tgaccacgga
     1681 cacggagctg ccgggcacgc gcgcgaacgc gggccccgac ttgacctccg tgacgtcgcc
     1741 agcgccgatg aagccccagc gcaccttctc gacaggctgg tcggacacgg cttccggtgg
     1801 cgtggcgtcg gcggccacta tctcgcccct cgcgtggtcg gcccgctcat gatgccttcg
     1861 ccacctgcga gccgatccac tctgggaggt acggcgaggt gcagcccttc gggacggggt
     1921 agtcgagata gaggccaagg cgctccccga ggtcgaggca gcgctgccgg cgttccgggt
     1981 cgtagatgcc gatcatcgcc gcgcagtagt tcatgaccca ctggaggtcc tggtccgcgc
     2041 caccgaggtc gcgctcgatg agccgcagga gccgctccgc ctcggccgtc gagctcttct
     2101 gctgacgcat gagccgcgcc ttgaacgacc agaagagccg ctggcgcatc agcgatcggc
     2161 tttgcagcca cccgtccatg ccggggtgca gcttcttggc ggccatcagc tggttggcga
     2221 gcagccattc gcccaccttg ccctgctccg ctctgtccag cgtctcgatg tcgaccagga
     2281 ggcgctccaa gtagtcgggc gtcaggctct tacggtccat gataaggatg gcgaccagct
     2341 tcgagttgac gttggcctcc tgccacaggc gctccgccag ctcctggtcg gtgccgatgc
     2401 gcttcgcgat ctcgcgcagc ttgcccatga gggtcgtagg cgagtcgagc gcctgcctga
     2461 cggcggggtc aagggtcttc gtcatgactc gcatagtagt atcctccccg gcatgacaac
     2521 gcgcgtacgg ggcacgaccg ggcgtgtcgc gctcgttacg ggcggtgcga gcggcatcgg
     2581 agccgcctgc gccgagcggc tcctcgccga cgggctcgat gtcgtcatag ctgatcgcga
     2641 cgcacaggct gctggggagc tggcctcacg cctcggctgt cgggcggtag tggcggacct
     2701 ggcgaagcga gagggctgcg cggacgccgt cgccgagacc ctcgagctgg ctggtgccct
     2761 cgatgtcctc gtcgccaacg ccggttatca acatatcgag gccctgagca ctttcccgga
     2821 agacaagtgg gacgacatgc tggccctcat gctcacgggc ccgttcctgt tgaccaagta
     2881 cgcctgggaa gccctcacgc gcagcggtaa cggtcgtctc atctacatgg gcagcgctca
     2941 cagcctcacg gcgagcccgt tcaagggcgc ctatgtgacg gccaagcacg gcctcgtcgg
     3001 cctagcccgc gtcgccgccc tggagggcgg ccaacacggc ataaccgcca acgtggtggc
     3061 gccggcctac gtgcgcacgc cgctcgtcgc cgggcagata gcggaccagg cgcgcacgcg
     3121 cgggatctcg cccgaggagg tggaaagcaa ggtgttcctc gagaacaccg ccatcaagcg
     3181 gctgctcgag ccggc
//
LOCUS       DAOQYE010000120         3191 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_32034_length_3191_cov_3.145089, whole genome shotgun sequence.
ACCESSION   DAOQYE010000120 DAOQYE010000000
VERSION     DAOQYE010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 3191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3191
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32034_length_3191_cov_3.145089"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..769
                     /locus_tag="PKN52_02975"
     CDS             <1..769
                     /locus_tag="PKN52_02975"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNQ98935.1"
                     /translation="LREAALASGVKDFLTKPFNPTEVRLRIRNLLEVRHYQRELVKHN
                     ERLEAAVRRRTQELELSQLEMLVRLARAAEYRDDSSGEHTWRVAHMCGVLARELGMAT
                     PEVELLLRAARLHDVGKITIPDGILLKAGSLTEAEYDVVKGHARAGAELLSGSRSPMM
                     RLAETIALTHHEWWDGRGYPQGLAGESIPIESRILAVADAFDALVTDHAHRKAKSPGE
                     AAAEIRAGSGTQFDPTVVVAFERAFAAGIFLPQTAFG"
     gene            825..1301
                     /locus_tag="PKN52_02980"
     CDS             825..1301
                     /locus_tag="PKN52_02980"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNQ98936.1"
                     /translation="MGTDDRVPPAAGEQADPIEVLQATPLRLAAIVETSSADWLESPY
                     GPGKWRRREVIAHLTDVELAFSYRMRQAVGEPGSAFTPFDQDAWARRNTRIDATVALE
                     AFRAMRALNLAWLASLDLQGWLAGGHHPVHGPQSVDDLVRYLAGHDLNHLEQLERP"
     gene            1344..1760
                     /gene="ndk"
                     /locus_tag="PKN52_02985"
     CDS             1344..1760
                     /gene="ndk"
                     /locus_tag="PKN52_02985"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002934976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HNQ98937.1"
                     /translation="MAVERTFAMVKPDGVRRRLVGNIVSRIEAKGYTVVGLKQLTISR
                     ELAERHYGEHKGKPFFDGLVSFITSGPVVAMVLEGENAIAGWRGMMGPTNPKDAPCGT
                     VRGDFATTIDENVAHGSDAPATAEREIGIFFPELQG"
     gene            1763..2923
                     /locus_tag="PKN52_02990"
     CDS             1763..2923
                     /locus_tag="PKN52_02990"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNQ98938.1"
                     /translation="MPGLSARVNSLKASSTVAFAARAKELARQGVDVISMTAGEPDFL
                     PPEHVLEAAREGIRLGLTKYTATEGTAELREAVAAKFKRENGLTYAPDQVLVSNGGKQ
                     TLYNAFMSVIEPGDEVVIVAPFWVSYPAQVELAGGVPVVVTARAEDGFVPDLDAVRAA
                     ITPRTKVLLINSPSNPTGAVFPPELVRGYAELAEEHDLWLFSDDLYEHLVYDGEFSTA
                     ATYVPHRTLIVHGASKGYALTGWRIGFGAGPKPLIAAMNRLQGQTTSGANSLAQYATV
                     AALNEVEKTAAFQAMTRAAYRERRDVLVSGLNRLGLSTPLPAGAFYVMADTTSIDPDE
                     ERAAIKLLDEARVAVVPGTDFLAPGKVRMSYATSLDQIREALQRIEALLVLG"
     gene            2948..>3191
                     /locus_tag="PKN52_02995"
     CDS             2948..>3191
                     /locus_tag="PKN52_02995"
                     /inference="COORDINATES: protein motif:HMM:NF013384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HNQ98939.1"
                     /translation="MANSRVDPQFHTIIEPFRIKSVEPIRFTTREERERALVAAGYNP
                     FNLHADDVLIDLLTDSGTGAMSSEQWAAMMRGDESYA"
BASE COUNT          522 a         1173 c         1053 g          443 t
ORIGIN      
        1 gctgcgcgag gccgccctcg caagcggcgt caaggacttc ctcaccaagc cgttcaaccc
       61 taccgaggtc aggctacgca tccgcaacct gctcgaggtg cggcactacc agcgcgagtt
      121 ggtcaagcac aacgaacgcc tcgaggcggc cgtcaggcgc cgcacccagg agctggagct
      181 ctcgcagctc gagatgttgg tgcggctcgc gcgcgccgcc gagtaccgcg acgactcgtc
      241 gggcgagcac acgtggcgcg tggcgcacat gtgcggcgtc ctggccaggg agctgggcat
      301 ggccaccccg gaggtcgaac ttctcctgcg cgccgccagg cttcacgacg tcggcaagat
      361 caccatcccc gacggcatcc tcctcaaggc cggcagcctc acggaggcgg agtacgacgt
      421 cgtcaaggga cacgcccgcg ccggcgccga gctcctctcc ggcagccgct cgcccatgat
      481 gaggttggcc gagaccatcg ccctcaccca ccacgagtgg tgggacggcc gcggctatcc
      541 gcaaggcctc gcgggggaga gcatccccat cgagtcgcgc atcctggccg tggcggacgc
      601 cttcgacgcg ctcgtcaccg accacgccca ccgcaaggcc aagagccccg gcgaggcggc
      661 ggccgagatc cgcgccggtt cgggcaccca gttcgacccc accgtggtgg tcgcgttcga
      721 gcgcgctttc gccgccggca tcttcctgcc gcagaccgcc ttcggctgac cgatggccgc
      781 tcgcagacga cgagccggcc ggcggcgctg taccctctag gaccatggga acagacgatc
      841 gagtaccgcc ggcggcgggc gagcaggccg acccgataga agtcttgcag gcgaccccgc
      901 tgcggctggc tgccatcgtc gagacgagct cggccgactg gctcgaatcg ccgtacggac
      961 cgggcaagtg gcgtagacgc gaggtgatcg cgcatctgac cgacgtcgag ctcgcgttct
     1021 cctaccgcat gcggcaggcg gtaggcgaac ctggctccgc gttcacgccc ttcgatcagg
     1081 atgcgtgggc gcgccgcaac acccgcatcg acgccacggt cgccctcgag gccttccgag
     1141 ccatgcgggc gctcaacctc gcctggctcg cgagcctcga tctgcagggg tggttggccg
     1201 gagggcacca tcccgtccac ggcccgcaga gtgttgacga cctggtccgt tacctggcgg
     1261 gccacgacct caaccacctg gagcaactgg agaggcccta gaggctcggg gagcccgcgg
     1321 tagacgtgat atcgtcagcc gttatggcag tcgaacgcac tttcgcaatg gtcaagcccg
     1381 acggcgtgcg ccgccggctc gtcggcaaca tcgtctctcg catcgaggcc aagggctaca
     1441 ccgtcgtcgg cctcaagcag ctcaccatca gccgcgagct ggccgagcgc cactacggcg
     1501 agcacaaggg caagcccttc ttcgacgggc tcgtctcgtt catcacttcc ggccccgtcg
     1561 tcgccatggt gctggaaggc gagaacgcca tcgccggttg gcgcggcatg atgggcccga
     1621 cgaacccgaa ggacgccccg tgcggcaccg tgcgcggcga cttcgccacg acgatcgacg
     1681 agaacgtggc ccacggctcc gacgcgccgg ccaccgcgga acgcgagata ggcatcttct
     1741 tcccggaact gcagggctga gcgtgccagg gctctccgcg cgcgtcaact cgctgaaggc
     1801 gtcgtccacc gtggcgttcg ccgcccgcgc caaggagttg gcgcgccagg gcgtggacgt
     1861 catctccatg accgcggggg agccggactt cctgccgccg gagcacgtgc tcgaggccgc
     1921 gcgggaaggc atcaggctcg gcctcaccaa gtacacggcc accgagggca cggccgagct
     1981 gcgcgaggcc gtcgccgcga agttcaagcg cgagaacggc ctcacctacg cccccgacca
     2041 ggtcctcgtc agcaacggcg gcaagcagac gctctacaac gccttcatgt cggtcatcga
     2101 gccgggcgac gaggtcgtca tcgtggcccc cttctgggtg agctacccgg cccaggtgga
     2161 gctcgccggt ggggtgcccg tggtcgtcac ggcgcgcgcc gaggacggct tcgtgcccga
     2221 cctcgacgcc gtgcgcgccg ccatcacgcc gcgcacgaag gtcctcctga tcaactcccc
     2281 gtcgaacccg acgggcgccg tgttcccgcc cgagctggtg cgcggctacg ccgagctcgc
     2341 cgaggagcac gacctctggc tcttctccga cgacctgtac gagcacctcg tctacgacgg
     2401 cgagttcagc actgcggcca cgtacgtgcc gcaccgcacg ctcatcgtgc atggcgccag
     2461 caagggctac gcgctcacgg gttggcgcat cggcttcgga gccggaccaa agccgctcat
     2521 cgcggccatg aaccgcctgc agggccagac gacctccggc gccaactccc tcgcccagta
     2581 cgccacggtg gccgccctga acgaggtcga gaagacggcc gccttccagg cgatgacgcg
     2641 cgccgcctac cgcgagcgcc gcgacgtgtt ggtgagcggc ctcaaccgcc tcggtctgag
     2701 cacgcccctg cctgccgggg ccttctacgt catggccgac acgacctcga tcgacccgga
     2761 cgaggagcgc gccgccatca agctcctcga cgaggcgcgc gtcgccgtcg tgcccggcac
     2821 ggacttcctg gcgcccggca aggtgcgcat gagctacgcc acgagcctcg atcagatccg
     2881 cgaggcgttg cagcgcatcg aggccctgct agtcctgggc tgagacgctg agcgaggggg
     2941 aggacacatg gccaacagcc gcgtcgaccc gcagttccac accatcatcg agccgtttcg
     3001 catcaagtcg gtcgagccga tccggttcac gacccgcgag gaacgggaac gcgcgctggt
     3061 cgcggccggg tacaacccgt tcaacctcca cgctgacgac gtcctgatcg acctcctgac
     3121 cgactccggc acgggggcca tgtcgagcga gcagtgggcc gccatgatgc gcggcgacga
     3181 gagctacgcc g
//
LOCUS       DAOQYE010000121         3163 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_32466_length_3163_cov_3.007400, whole genome shotgun sequence.
ACCESSION   DAOQYE010000121 DAOQYE010000000
VERSION     DAOQYE010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 3163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3163
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32466_length_3163_cov_3.007400"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="PKN52_03000"
     CDS             <1..211
                     /locus_tag="PKN52_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98940.1"
                     /translation="ETAFPQPAWVEGRLRMAAHPYRGPFIASYWFGDEAVREVRERVP
                     AERFGAFVEYLYGSLNTVQSMKLFV"
     gene            215..1015
                     /locus_tag="PKN52_03005"
     CDS             215..1015
                     /locus_tag="PKN52_03005"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HNQ98941.1"
                     /translation="MEDARVAVSDRSHDRSGSVRAVERALRLIEILARSRAPFSITDL
                     TIEAGLPASTVHRLVQSLMSLGYVTQHQASKRYGVGRGIADLNRAMLLKHEFSQHAQP
                     YLERLVDETKESASLAALYGTSIIYLNHVESPLIMRVSTPVGTLAPLHCTASGKVFLA
                     DFGPELFEEVVRFSGLEAYTGSTISTRDELVEHLRGVERQGYALDDEEYEQGARCIAV
                     GLRGSSGAVSAAISISGPSIRMTDDRVPELAVLVRSVADEFAQEMREP"
     gene            1065..2093
                     /locus_tag="PKN52_03010"
     CDS             1065..2093
                     /locus_tag="PKN52_03010"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HNQ98942.1"
                     /translation="MTEVLICSYVEPELVERVCGVDPGVRVHYRPELLPRPRYIADHV
                     GTALVRTPAEQADWDSLLARAEVLFDFDYTDIAGWARKAPGVRWLQASSAGIGQLVKR
                     HRLDETGIVFTTASGVHAKPLAEFVMMIVLEHVKRAGLARDQQARRSWQRFTTGELSG
                     KTLGIVGLGRIGKEVARLAKAFGMRVIASKRAVGGRDPADLGVDALYGADGLHDLLAE
                     ADYVCLVTPHTPETEGLMNEAAFAAMKPGAVLVNIARGAVVREPALLAALDSGRLAHA
                     ALDVAAVEPLPETSPLWTHPNVTIYPHSASTGEHENERLVELFCENLRRYLAGEPLLN
                     RLDTKRMY"
     gene            2097..>3163
                     /locus_tag="PKN52_03015"
     CDS             2097..>3163
                     /locus_tag="PKN52_03015"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HNQ98943.1"
                     /translation="MDRLEIPTHEHEARLAALRRTLEAAGFSAMCVFGPVRISYLTGF
                     HFAATERPVVLVVPVDADPHILIPQLEVDHVRQQARRLEHVRTYPEYPGGGSGKHPMS
                     HLMDLLADAGLANASLTADLDGYEHRWGYRGPRLSQALGREVATHLEFVDDLRIVKSP
                     AEVALVREACVWGDRAHRVMQDLMVEGASELRVSQEASLTTTGEMLRELGDRYVPKSR
                     EGMPANAMFIRGSNTANPHGLHGNEGLRGGDVIITGAFGVVGGYESELERTMFFGRPA
                     EACVTYFEAMLAAQNVALAALRPGRTCADVEREVSGFIRDELGMGRYLRHHTGHSFGM
                     EGHEHPFLDLDDHTPIRAGMIL"
BASE COUNT          509 a         1056 c         1087 g          511 t
ORIGIN      
        1 ggagacggct ttcccccaac ccgcttgggt ggagggtcgg ctgcgcatgg ccgcccaccc
       61 ttaccgcggc ccgttcatag cctcgtactg gttcggcgac gaggccgtcc gggaagtccg
      121 cgagagggtt ccggccgagc gcttcggcgc cttcgtcgaa tacctctatg gtagcctcaa
      181 caccgttcag agcatgaagc tgttcgtttg agccatggaa gatgcgcggg tggcagtttc
      241 cgatcgatcc cacgaccgtt ccggctccgt gcgcgccgtg gagcgagcgc ttcgcttgat
      301 cgagatactg gcgcgctctc gtgcgccctt ctccatcacc gacctgacga tagaggccgg
      361 gctgcccgct agcacggtgc ataggctcgt gcagagcctg atgtcgctcg ggtacgtcac
      421 gcagcatcag gcgtcgaagc gctacggggt gggacgtggc atagccgacc tgaacagggc
      481 gatgctcctg aagcacgagt tcagccagca cgctcagccg tacctcgagc gcctggtgga
      541 cgagaccaag gagtccgcta gtctggccgc cctgtacggg acgtccatca tctacctgaa
      601 ccacgtcgag tcgcccctca tcatgcgggt gtcgacgcca gtcggtacct tggcgccgct
      661 gcattgcacg gcgtccggca aggtcttcct ggccgacttc ggccccgagc tgttcgagga
      721 ggtcgtccgc ttctcgggcc tcgaggccta cacgggtagc accatcagca ctcgcgacga
      781 gctcgtcgag catctgaggg gagtcgaacg ccaaggttac gcgctcgacg acgaggagta
      841 cgagcagggt gcacgctgca tcgccgtcgg gctgcgcggc agttccgggg cggtgagcgc
      901 agccatcagc atctccggac cgtctatccg gatgacggac gaccgcgtgc cggaactagc
      961 cgtgctcgtg cgctcggtcg ccgacgagtt cgcgcaagag atgcgcgagc cgtgacctcg
     1021 agggcggcgg ctcaggtacc gccccgactg tccaggagaa cgagatgacc gaggtactga
     1081 tctgttccta cgtcgagccc gagctggtcg agcgcgtgtg cggcgtcgac cctggggtcc
     1141 gtgtgcacta ccgacccgag ctgctgccgc ggccgcgtta catcgcggac cacgtcggca
     1201 cggcgctcgt ccgcacgccg gccgagcagg cggactggga ctcgctcctc gcgcgcgccg
     1261 aggtcctctt cgacttcgac tacaccgaca tcgccggctg ggcgcgcaag gcgccgggcg
     1321 tgcgctggtt gcaggcttcc agcgccggga tagggcaact ggtcaagcgc catcgcctag
     1381 acgagacggg catcgtcttc accaccgcca gcggcgtgca tgccaagccg ctggccgagt
     1441 tcgtcatgat gatcgtgctg gagcatgtga agcgcgccgg gttggcgcgc gaccagcagg
     1501 ctcggcggtc gtggcagcgc ttcaccacgg gcgagctgtc cggcaagacg ctcggcatcg
     1561 tcggcctggg ccggatcggc aaggaagtgg cccggttggc gaaggccttc ggcatgcgcg
     1621 tgatcgccag caaacgcgcg gtaggaggcc gggaccctgc tgacctcggg gtcgacgccc
     1681 tctacggcgc ggacggcttg cacgacctgc tcgccgaggc ggattacgtg tgtctcgtga
     1741 cgccccatac tccggagacg gagggcctga tgaacgaggc ggctttcgcg gccatgaagc
     1801 ccggtgccgt cctcgtgaac atcgcgcgcg gtgccgtggt gcgcgagccg gccctgctgg
     1861 ccgccctcga ctccgggcgc ttggcgcatg cggcgttaga cgttgcggcg gtggagccgc
     1921 tgcccgagac gagcccgctc tggacccacc cgaacgtcac catctacccg cactccgcga
     1981 gcacaggaga gcacgagaac gagcgactcg tagagctctt ctgcgagaac ctgcggaggt
     2041 acctggccgg agagccactg ctgaaccggc tcgacacgaa gaggatgtat tgaggcatgg
     2101 accggctcga gatccccacc catgagcacg aggcacgcct cgcggcgttg cggcgcaccc
     2161 tcgaggccgc cggcttctct gcgatgtgcg tcttcggacc cgtccgcatc agttacctca
     2221 ccggcttcca cttcgccgct accgagcgcc ccgtcgtcct cgtggtgccc gtggacgccg
     2281 atccgcacat cctcatcccg cagctcgagg tcgaccacgt tcggcagcag gcgaggcggc
     2341 tcgagcacgt gcgcacgtac cccgagtacc ctggcggggg ctcgggcaag cacccgatgt
     2401 cgcacctgat ggacctgctc gcggacgctg ggctggcaaa cgcctccctc accgccgacc
     2461 tggacggcta cgagcatagg tggggttatc gcggccctag gctgagccaa gcgcttggca
     2521 gggaagtggc gacgcacctg gagttcgtgg acgatctccg catcgtcaag agccccgccg
     2581 aggtcgcgct ggtgcgcgaa gcgtgcgtat ggggcgaccg cgcgcaccgc gtcatgcagg
     2641 acctgatggt cgagggcgcc agcgagctcc gtgtctcgca agaggcgagc ctcacgacca
     2701 cgggcgagat gctgcgcgag ctcggagacc gctacgtccc gaagtcgcgt gagggcatgc
     2761 ctgcgaacgc gatgttcatc cgcgggagca acaccgccaa cccgcacggg ctgcacggga
     2821 acgagggctt gcgcggcggg gacgtgatca taacgggcgc cttcggggtc gtcggcgggt
     2881 acgagtcgga actcgagagg accatgttct tcgggcggcc ggccgaggct tgcgtaacgt
     2941 atttcgaggc catgttggcg gcccagaacg tcgcgctcgc cgcgctacgc cccggccgca
     3001 cttgcgcgga cgtcgagcgg gaggtgtccg gcttcatccg agacgagctg ggcatgggtc
     3061 ggtacttgcg tcaccatacg ggccacagtt tcggcatgga ggggcatgag cacccgttcc
     3121 tcgacctcga cgaccacacg ccgatacgag ccgggatgat cct
//
LOCUS       DAOQYE010000122         3156 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_32580_length_3156_cov_3.160916, whole genome shotgun sequence.
ACCESSION   DAOQYE010000122 DAOQYE010000000
VERSION     DAOQYE010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 3156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3156
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32580_length_3156_cov_3.160916"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            143..382
                     /gene="acpP"
                     /locus_tag="PKN52_03020"
     CDS             143..382
                     /gene="acpP"
                     /locus_tag="PKN52_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNQ98944.1"
                     /translation="MDEHEILEKIKDVVADKLDADPSDVTEESRFMDDLGADSLDVVE
                     LIMGLEDEFGVEISDEEAEGIRTVGDAVRFIADKQ"
     gene            499..1728
                     /gene="fabF"
                     /locus_tag="PKN52_03025"
     CDS             499..1728
                     /gene="fabF"
                     /locus_tag="PKN52_03025"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HNQ98945.1"
                     /translation="MRRVVVTGVGPVTPIGVGAAEFLAAQHVAKNGIRPITLFDASDL
                     SVRFAGEVDVDVAAYLEPKEARRLDRFVQFALIGADLALADSGLSAEEVAGEHTGTCV
                     GSGIGGLATWEEQSKTRFERSPMRISPFFIPMLIANMASGHVAMRFGLTGPSSSVVTA
                     CATGSGNIGDGYRMIQRGEADIVFAGGSEAPVTPMGIGGFAVMKALSTRNDSPETASR
                     PFTASRDGFVAGEGAGILILEEYERAVKRGARIYAEIVGYGTSADAHHITAPAPGGTG
                     AIAAIRRALKDAKITPDQVGYINAHGTSTEANDLNETLAFKAVWEGHGPVPPVSSTKS
                     MTGHTLGAAGGIEGIATVQAIHYGVLPPTRNYIDPDPVLDLDYIPEGAREKRVDVAIS
                     TNFAFGGQNACLVFKRV"
     gene            complement(1779..>3156)
                     /gene="murJ"
                     /locus_tag="PKN52_03030"
     CDS             complement(1779..>3156)
                     /gene="murJ"
                     /locus_tag="PKN52_03030"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HNQ98946.1"
                     /translation="NLFRELLAEGALTNSFIPVYKGLEPRDARRMAGALLALLMLVNG
                     LLLVGAYLAAPLLARLLISSAGNVDLELTTRLVRIVFPFLPAISLSALAMGILNAEER
                     FLAPAWAPVALNVVTVVLMALFPGQAVMLAWAHVLGGAAQLLVQVPALVRSGLMPRLG
                     ALWHPALGSVLLLMLPFAFTAGGRQVLNVVASNVITAIDAGAQGAFYLADLFLSLALG
                     LFSISPALAYYSRLSDHAVHEPESLAPTLAEGLRFITFLTVPAGLALALLAGPAVDAV
                     YNWRSLLGEPMDAQLRAFTIAATLPLGFAVFPIGAFNLLIRTFYVRRIVRTPVVLVLA
                     FLTLQGVLYVVLSRAFGIAGVAWATAVAAWLQLLVAAWLVERREGFGLGRWASGALKV
                     WLAAGLATGAAALALAVLPIGAGWWSQVGRLAVGGGVLLAVFAAAGALLGLPELAAVL
                     RRLRPSGR"
BASE COUNT          574 a         1104 c         1100 g          378 t
ORIGIN      
        1 agggccccgt ggccggcggg gcgtgagccc accgttcacg agggcgcaac cgcggtgttg
       61 gcacggcaat tacccgtgct acagtctcgc aagaccagca ctacatgagc acggcacgaa
      121 cagttgccaa ggagggaaag tcgtggacga gcacgagata ctggagaaga tcaaggacgt
      181 ggtcgctgac aagcttgacg ccgacccgag cgacgtcacc gaggagtccc gcttcatgga
      241 cgacttgggc gccgactccc tcgacgtcgt cgagctgatc atgggcctcg aggacgagtt
      301 cggggtcgag atctccgacg aggaagccga aggcatccgc acggtcgggg acgccgtccg
      361 cttcatcgca gacaagcagt gaggaccggc cccggctcga gcttgaccgc tcacgcgcgg
      421 ggccacggtc gcagcacggt cgacggcgca ccgtgcgtca cgctcgctcg ccgttgcaac
      481 cgttaactta ggacgaccat gagacgagtc gttgtcaccg gtgtcgggcc cgtaacgccg
      541 atcggcgtag gcgccgccga gttcctggcg gcccagcacg tggccaagaa cggtatcaga
      601 cccatcaccc tgttcgatgc cagcgacctc tccgtgcgtt tcgctggcga ggtcgacgtc
      661 gacgtggccg cctacctgga gcccaaggag gccaggcgcc tcgaccgctt cgtgcagttc
      721 gcgctcatcg gggcggatct cgccttggcg gactccggcc tgagcgccga agaggtcgcg
      781 ggcgagcata cgggcacgtg cgtcggctcc ggcatcggcg gcctcgccac gtgggaggag
      841 cagtccaaga cccggttcga gcgctccccc atgcgcatca gccccttctt catccccatg
      901 ctcatcgcca acatggcaag cgggcatgtc gccatgcgtt tcggcctcac ggggccgagc
      961 tcgagcgtcg taacggcctg cgccaccggc tcgggcaaca tcggcgacgg ctaccgcatg
     1021 atccagcgcg gggaggccga catcgtcttc gcgggcggct ccgaggcgcc cgtcaccccc
     1081 atggggatcg gcggcttcgc cgtcatgaag gccctgtcca cccgcaacga cagccctgag
     1141 acggccagcc ggcccttcac cgccagccgc gacggcttcg tggccggcga gggcgctggc
     1201 atcctgatcc tggaagagta cgagcgcgcc gtcaagcgcg gcgcccgcat ctacgccgag
     1261 atcgtcgggt acggcacgag cgccgacgcg caccacatca cggcgccggc gccgggcggc
     1321 acgggcgcca tcgcggccat caggcgcgcg ctcaaggacg ccaagatcac ccccgatcag
     1381 gtcggttaca tcaacgccca cggcactagc acggaagcga acgacctcaa cgagacgctc
     1441 gccttcaagg ccgtctggga gggccacggc ccggtgccac ccgtctcctc gaccaagtcg
     1501 atgacggggc acacgttggg ggcggccggc ggcatcgagg ggatcgcgac ggtgcaggcc
     1561 atccactacg gcgtcctgcc ccccacgcgc aactacatcg accccgaccc cgtcctcgac
     1621 ctcgactaca tccccgaggg cgcgcgcgag aagcgggtcg acgtggccat ctccaccaac
     1681 ttcgccttcg gcggccagaa cgcctgcttg gtattcaagc gcgtctgagt cctcaagcgc
     1741 gtctaagtct tcaagcccat ctgagtcttc aagcgcgtct agcgacccga cgggcgtaac
     1801 cgcctgagca ccgcggctag ttcaggcagc ccaaggaggg cgccggccgc cgcgaagacc
     1861 gccagcagca ccccgccccc cacggccagg cgcccgacct ggctccacca gccggcgccg
     1921 ataggtagaa cggcgagggc cagggcggcc gcgcccgtag ccaagccggc cgcgagccac
     1981 accttcaagg cccccgacgc ccaccggccg aggccgaagc cctcgcgccg ctcgacgagc
     2041 caggcggcga cgaggagctg cagccaggcc gccacggccg tcgcccacgc cacgccggcg
     2101 atgccgaacg cgcgcgacag gacgacgtac agcaccccct gcagcgtcag gaacgcgagg
     2161 acgagcacga ccggggtgcg cacgatgcgc ctgacgtaga acgtccggat gagcaggttg
     2221 aaggcgccga tggggaacac ggcgaagcca agcggcaacg tggcggcgat ggtgaacgcg
     2281 cgcaactggg cgtccatggg ctcgccgagg agcgagcgcc agttgtagac cgcgtcgacg
     2341 gccggtccgg ccagcaacgc gagggccagg cccgccggca ccgtcaggaa cgttatgaac
     2401 cgcagaccct ccgctagggt gggggcgagc gactccggct cgtgcacggc gtggtcggag
     2461 aggcgcgagt agtacgcgag cgccggcgag atgctgaaga gcccaagcgc caggctgagg
     2521 aacaggtcgg cgaggtagaa ggcgccttgc gccccggcgt cgatggccgt gatgacgttg
     2581 gacgcgacca cgttgagcac ctgccgcccg ccggccgtga acgcgaacgg gagcatgagg
     2641 agcagcacgg agccgagcgc cgggtgccag agcgcgccga gccgcggcat gaggccggag
     2701 cggaccaagg ccgggacctg cacgaggagc tgcgcggcgc cccccaggac gtgcgcccac
     2761 gcgagcatca cggcctggcc agggaagagg gccatgagca cgaccgtgac gacgttgagc
     2821 gcgacgggcg cccaggcggg cgcgaggaag cgctcctcgg cgttcaggat ccccatcgcc
     2881 agcgccgaca acgagatggc cggcaggaac gggaagacga tccgcacgag cctggtcgtg
     2941 agctcgaggt ccacgttgcc ggccgacgag atgagcaggc gcgcgaggag cggcgcggcc
     3001 aggtaggcgc ccactagcag caggccgttg acgagcatga ggagggcaag aagcgccccg
     3061 gccatgcgcc tcgcgtctcg cggctcgaga cccttgtaga cggggatgaa cgagttggtc
     3121 agcgccccct cggccagcag ctcgcggaag aggttg
//
LOCUS       DAOQYE010000123         3141 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_32808_length_3141_cov_2.776410, whole genome shotgun sequence.
ACCESSION   DAOQYE010000123 DAOQYE010000000
VERSION     DAOQYE010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 3141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3141
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32808_length_3141_cov_2.776410"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /gene="rpmE"
                     /locus_tag="PKN52_03035"
     CDS             complement(<1..207)
                     /gene="rpmE"
                     /locus_tag="PKN52_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HNQ98947.1"
                     /translation="MKKNIHPKMVPCKIICDGQVVMETYSTKPELHVDVWSGNHPFFT
                     GEQRFIDTEGRVEKFQKRFGDSYRQ"
     gene            complement(294..710)
                     /locus_tag="PKN52_03040"
     CDS             complement(294..710)
                     /locus_tag="PKN52_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HNQ98948.1"
                     /translation="MSIPAPLQGIVDEFRSLPASQRLPLLLEYANKLPSPPAGMQAEL
                     ERVHECQTPLFLKAEVGTDGVVKLYFDAPRESPTTRGFASVVSAGLLGATVPEVLAVP
                     EDFYEDMGLAELISPLRLRGMGAIVGRVKRQLAKAG"
     gene            772..2079
                     /gene="aroA"
                     /locus_tag="PKN52_03045"
     CDS             772..2079
                     /gene="aroA"
                     /locus_tag="PKN52_03045"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HNQ98949.1"
                     /translation="MVRLPSTVEIGPQGPVRGGLHVPGSKSITNRALVVAALADGTTT
                     LRGALVAEDSEVLVRALSSLGVAVESLGSDVRVRGAGGPFPAKTANLDLRLSGTSIRF
                     LAAAVAVGHGRFTLDGTERMRERPIQETLDAVTALGGEARSLRGNGCPPIEIVASGLR
                     GGSVRVRGDRSSQFLSGLLMAAPYASGPVTVEVDGELLSKPFVDMTLDVMRTFGVAVK
                     RDGYRAFTVSPGHYRGLDYAVEGDAMAAGYFWAAAGVTGGRVRVNNLGKGTRQGDARL
                     LDVLEELGCEVCRGEHHVSVEGPRGGRLRGGQDFDLNDMPDQAQTLAVAALFADAPVR
                     ISNVGNLRIKETDRLSAMRMELTRLGAYVEEGDDYLVVHPIETPPVAPVALETYGDHR
                     MAMALAVAGARLPNVVIKDPSCVVKTYPRFFEDFLGLLAGRLA"
     gene            2076..2798
                     /gene="cmk"
                     /locus_tag="PKN52_03050"
     CDS             2076..2798
                     /gene="cmk"
                     /locus_tag="PKN52_03050"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HNQ98950.1"
                     /translation="MSSAGDSAAAGATGRAAADQGTPARTVITIDGPAASGKSSVARA
                     LAGQLGFAYVSSGLLYRAATFVAMGAGVDESDEDALLGLLAHRAVTLTPDALGNRVSV
                     DGEDVTAGLHTDAVDVRVSTVAKHGRVRDWVNERLRELGGSFVIDGRDMGTVVFPDAA
                     AKFYLTADPRIRAERRASERGSDLDAIAAELERRDERDRSQSVPAADATVVDTGNMSL
                     DEVVARVRADVLRALQDAAVAG"
     gene            2795..>3141
                     /locus_tag="PKN52_03055"
     CDS             2795..>3141
                     /locus_tag="PKN52_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006680938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HNQ98951.1"
                     /translation="MTVPGVFARPASALRLVIVTSSRYKPEAHPLARQLGDLFERAGA
                     KVVLDLGGGAELSGYSREADLAVSVGGDGTLLAAARRLAGSDVPLMGVNLGKLGFLAE
                     FGAEEAIAYASGA"
BASE COUNT          442 a         1089 c         1125 g          485 t
ORIGIN      
        1 ctgacggtac gagtcgccga agcgcttctg gaacttctcg acgcggcctt ccgtgtcgat
       61 gaagcgctgc tcgcccgtga agaacgggtg gttgcccgac cagacgtcga cgtgcagttc
      121 cggcttggtg ctgtacgtct ccatgacgac ctggccgtcg cagatgatct tgcatggcac
      181 catcttgggg tggatgttct tcttcatctt gagcttcctc cacgggcgag cgcccggcta
      241 agtcaacctt gagatagtag cacgcgggag ccgtgttcgg ggctgcctcg cgcttacccg
      301 gccttggcga gctggcgctt gacgcgcccg acgatcgcgc ccatgccgcg caggcgcaag
      361 ggcgagatga gctccgccag ccccatgtcc tcgtagaagt cctccggaac ggcgagcacc
      421 tcgggcaccg tggcgccgag gaggccggcg ctgaccacgg acgcgaagcc gcgcgtcgtc
      481 ggcgactccc tcggggcgtc gaagtagagc ttcacgacgc cgtcggtccc cacctcggcc
      541 ttgaggaaga ggggggtctg acactcatga acccgttcga gctcggcctg catgccggcc
      601 ggaggggaag ggagcttgtt ggcgtactcg agcagaagcg gcaggcgctg ggaagccggc
      661 agcgacctga actcgtccac gatgccttgc agtggggctg ggatgctcac gggggcagcc
      721 taccccggtc ccgaaggcgc gtcaccaccc ggtgtatcct tgaccacgct gatggtacgc
      781 ctcccctcca cagtcgagat cggcccgcaa gggcccgtgc gcgggggcct gcatgtccct
      841 ggttcgaaga gcatcaccaa cagggctctc gtcgtcgcgg ccctcgccga cggaaccacg
      901 acgctgcgcg gcgcgctcgt cgccgaggac tccgaggtgc tggtccgcgc gctcagctct
      961 ctcggggtcg ccgtggaatc gctcggtagc gacgtgcggg tgcgcggtgc cggggggccg
     1021 ttccccgcca agaccgccaa cctcgacctg cggctctccg gtactagcat ccgcttcctc
     1081 gccgctgccg tcgccgtcgg ccacggccgc ttcactctgg acggcaccga gcgcatgcgc
     1141 gagagaccga tccaggagac cctcgacgcc gtgactgccc tcgggggcga ggcacgcagc
     1201 ctgcgtggca acggctgccc gccgatcgag atcgtcgcct cgggtctgag gggcgggagc
     1261 gtgagggtac ggggcgaccg gagctcccag ttcctctccg gcttgctcat ggctgctccg
     1321 tacgcctccg gtcccgtgac cgtcgaggtc gacggcgagc tcctgtccaa gccgttcgtc
     1381 gacatgacgc tcgacgtcat gcgcacgttc ggcgtcgcgg tcaagcgcga cggctaccgc
     1441 gccttcaccg tttcgcccgg gcactaccgt gggctcgact acgccgtgga gggcgacgcg
     1501 atggcggccg gctacttctg ggccgccgcc ggggtcaccg gcggacgcgt gcgcgtcaac
     1561 aacctcggca agggcacccg acagggcgac gcgcgcctcc tcgacgtgct ggaggagctg
     1621 ggttgcgagg tatgccgcgg cgagcaccac gtcagcgtcg agggtccccg cggcggccgc
     1681 ttgcgcggcg gccaggactt cgacctcaac gacatgcctg accaggcgca gaccctcgcc
     1741 gttgcggcgc tcttcgccga cgcgcccgtc cgcataagca acgtcggcaa cctccgcatc
     1801 aaggagacgg accgcttgag cgccatgcgg atggagctga ccaggctcgg cgcgtacgtg
     1861 gaggagggcg acgactacct cgtcgtccac cctatcgaga cgccgccagt ggcgccggtg
     1921 gcactggaga cctacggcga ccaccgcatg gccatggcgc tcgccgtcgc cggcgcgcgc
     1981 ctgccgaacg tcgtcatcaa ggacccgagt tgcgtcgtga agacctatcc gcgcttcttc
     2041 gaggacttcc tggggttgct ggccgggcgg ttggcgtgag ttccgccggc gactccgcag
     2101 ccgcgggcgc caccggccga gcagcggccg accagggcac gccggcccgg acggtcatca
     2161 ccatcgacgg gcccgcggcg tccggcaagt cgagcgtcgc gcgcgccctc gccgggcagc
     2221 tgggcttcgc gtacgtgtcg agcgggctcc tgtaccgagc ggcgacgttc gtggccatgg
     2281 gcgccggcgt cgacgagtcg gacgaggacg ctctcctggg cctcctggcg caccgcgccg
     2341 tcacgttgac gccggacgcg ctgggtaacc gggtcagtgt cgacggcgag gacgtgacgg
     2401 ccggcttgca cacggacgcg gtcgatgttc gcgtctcgac ggtggccaag catggcaggg
     2461 tgcgcgattg ggtgaacgag cggttgcgcg agctcggtgg ctccttcgtg atcgacgggc
     2521 gcgacatggg gaccgtggtg ttcccggacg ccgcggcgaa gttctacctg acggccgacc
     2581 caaggatccg ggccgagcgc agggccagcg agcgcggcag cgacctggat gccatcgcgg
     2641 cggagctgga acggcgcgac gagcgcgaca ggtcgcaatc ggttcctgcg gccgacgcca
     2701 cggtggtcga cacggggaac atgagcctcg acgaggtcgt ggcgcgcgtt cgggccgacg
     2761 tgctgcgggc ccttcaggac gcggcggtgg cgggttgacc gtccccggcg tcttcgcccg
     2821 cccggcctca gcgttgcgcc tcgtcatcgt cacgagcagc cgctacaagc ccgaggcgca
     2881 cccgctcgcc aggcagctgg gcgacctgtt cgagcgcgcc ggtgccaagg tcgtcctcga
     2941 cctcggcggc ggcgcggagc tgagcggcta ctcgcgcgag gccgacctgg ccgtctctgt
     3001 cggcggcgac ggcaccctct tggctgcggc gcggcgtctg gccggctccg acgtgccgct
     3061 catgggagtc aaccttggca aactgggttt cctggcggag ttcggcgcgg aagaggccat
     3121 cgcctacgct tccggggccg a
//
LOCUS       DAOQYE010000124         3123 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_33093_length_3123_cov_3.824967, whole genome shotgun sequence.
ACCESSION   DAOQYE010000124 DAOQYE010000000
VERSION     DAOQYE010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 3123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3123
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33093_length_3123_cov_3.824967"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="PKN52_03060"
     CDS             complement(<1..488)
                     /locus_tag="PKN52_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HNQ98952.1"
                     /translation="MPEADFLADPALVQRSVNAVRALTIDATQAAGDGHPGMPLGAAA
                     MGYTLFAHTMRYDPRDPAWPDRDRYVQSAGHGSMLLYSLLHLTGFELPMDELRRYRQW
                     GSRTPGHPERGHTAGVETTTGPLGQGISTAVGMALAEAHLAARYNRPGFTVVDHHTYV
                     IAS"
     gene            complement(622..1119)
                     /locus_tag="PKN52_03065"
     CDS             complement(622..1119)
                     /locus_tag="PKN52_03065"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HNQ98953.1"
                     /translation="MANGTERESEWVVSVQSAFPNMANPLGTLFGGHVMQLMDIGAAI
                     AAQRFCRQVVVTASTEPIDFRNPIHVGEIIELKCRVAWTGRTSMIVRCEVHGENALTG
                     ERRLCTIGHLNFVAIGPDGRPTPVPTLRVGTELERSHWETAARVREDIERRRDRKAYE
                     PTTLA"
     gene            1188..1880
                     /locus_tag="PKN52_03070"
     CDS             1188..1880
                     /locus_tag="PKN52_03070"
                     /inference="COORDINATES: protein motif:HMM:NF014669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015225 - biotin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015878 - biotin transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HNQ98954.1"
                     /translation="MTRSQLTSPLINRLVPAPARQVEPARQVEPARQAAPARQAAPAR
                     QAAPAALSVFAAGRLRQVALVAAGVAFLALLAQLRVQVGPVPVTGQTLGVLLLGAAYG
                     LRLGAVATGAYLLLGAAGLPLFTGGQAGVAYALGPTGGYLVGFVVAAALLGALAERGW
                     DRSLGSAALAMFLASCVIYACGLAWLHGVIGGTWLRTVQVGLVPFLLGDLLKLLIAAA
                     ALPAAWRLVGRR"
     gene            2520..>3123
                     /locus_tag="PKN52_03075"
     CDS             2520..>3123
                     /locus_tag="PKN52_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-binding protein"
                     /protein_id="HNQ98955.1"
                     /translation="MLSIPEKLLFVLLAATSAYLAYLGFRRVVQVIARGQTGQVPRLN
                     QLPRRVFDGIMRTLGQTTVFRARPVASFFHSFIFYGFILYLLVNVVDAINGLLPPSFT
                     ARFGFGVVGDLYRLVADVLTLLILVGMVYFLVRRFLAKPKTMEYGARVKLHEDVRAGG
                     VKRDSLIVGSFILLHVGSRLLAESFLLASHGHSDPYQPMAS"
BASE COUNT          399 a         1084 c         1092 g          548 t
ORIGIN      
        1 gatgcgatca cgtaagtgtg gtggtcgacg accgtgaagc ccggccggtt gtagcgcgcg
       61 gccaagtggg cctcggccag cgccatgccg acggccgtgc tgatgccttg gccgagcggc
      121 cccgtcgtcg tctcgacccc ggccgtgtgc ccccgctccg ggtgcccggg cgtacgcgag
      181 ccccactgcc ggtagcgcct caactcgtcc atggggagtt cgaagccggt caggtgcagg
      241 agagagtaga ggagcataga gccgtggccg gccgactgca cgtagcgatc tcggtcgggc
      301 caggcggggt cgcgcgggtc gtagcgcatg gtgtgggcga agagcgtata gcccatcgcc
      361 gccgcgccga ggggcatgcc tgggtggccg tcgccggcag cctgcgtggc gtcgatggtg
      421 agggcgcgaa ccgcgttcac ggagcgttgc acgagcgcgg ggtcggcgag gaagtcggct
      481 tctggcatgg gactgtccct tcgagctagg agcggcgccg gcaggaaccg cgtggcggcg
      541 cgcgacgtcg taaacgatac cagggcccga ggggccggtt caggagcgcg gcgagcgtcc
      601 cggctttcgg cgcatggtcc gtcaggcgag cgttgtcggc tcgtacgcct tccggtcgcg
      661 gcgcctctcg atgtcttcgc gcaccctagc ggccgtctcc cagtggctgc gctccagctc
      721 cgtgcccact ctcagggtcg ggacgggcgt ggggcgcccg tccgggccga tggccacgaa
      781 gttgaggtgc ccgatcgtgc agaggcggcg ttcgcccgtc agcgcgttct cgccgtgcac
      841 ttcgcagcgc acgatcatgc tcgtgcgccc ggtccacgcg acccggcact tgagctcgat
      901 gatctcgccg acgtggatgg ggttgcggaa gtcgatgggc tccgtggacg ccgtgacgac
      961 cacctggcgg cagaaccgct gcgccgcgat ggcggcgcct atgtccatca gctgcatgac
     1021 gtgaccgccg aagagggtgc caagcgggtt ggccatgttc gggaaagccg actggaccga
     1081 gaccacccat tccgactcgc gttccgttcc gttcgccatc ggtcgagtat aggcgctgct
     1141 atgctgccgc aggcgcggcc cttcacccgg ccgccggagg ccagcacatg acgagaagcc
     1201 aactgacgtc cccgttgatc aacaggctgg tgccagcgcc ggcgcggcag gtcgagccgg
     1261 cgcggcaggt cgagccagcc cggcaagctg cgcctgcccg gcaggccgcg cctgcccggc
     1321 aggccgcgcc tgccgccctg tccgtgttcg cagccggccg gctgaggcag gtggcgctcg
     1381 tggctgccgg cgtcgcgttc ctggcgctgc ttgcccagtt gcgcgtccag gtaggccccg
     1441 tccccgtcac cggccagaca ctcggcgtgc tcctgcttgg cgccgcttac ggcctccgcc
     1501 taggcgcggt cgcgaccggc gcgtacctgc tcctcggcgc ggccgggctc ccgctcttca
     1561 cgggcggtca ggcgggcgtg gcgtacgctt tggggccgac gggtggctac ctggtcggtt
     1621 tcgtcgtcgc ggccgccctg ctcggcgctc tcgccgaacg cggttgggat cgctcgctcg
     1681 gctcggcggc cctcgccatg ttccttgcca gctgcgtgat ctacgcttgc ggtctcgcct
     1741 ggctccacgg cgtcataggc ggtacatggc tgcgcaccgt tcaggtcggg ctcgtcccgt
     1801 tcctcctcgg tgacctgctc aagctgctga tcgctgcggc agcgttgccg gccgcctggc
     1861 ggctcgtcgg tcgacgctga ggcgctcagc ccgggcgctc cttcaacggg cctgaagccg
     1921 cgtagctcat tactgcttta cccggctccg acccgcgttt acccgactcc ggtccgcgcc
     1981 gccgcccgtg ctcaggaggc ggcatcaggg aacggggcgg catcactaaa aactcgcccc
     2041 cggcctcttc ggtcgggggc gagtcggtgc cggcctggaa ctcggggaac cctcgtggtt
     2101 ccgactcatc caaggggcgg tcctggggct accctaggcg ggcacctcca ctggtcattg
     2161 ctatgcgttg aataagacca cctcctttcc gtggtagcga catattagtc ctaccggacc
     2221 gggggtgtgt caagtgcgct actcgcggta ggtacaccga cgggtcgtcc tcccgggagg
     2281 ctcgactttg gaggtaggta caccgaccga ggcgcctgcc cggggtacta cactggctgt
     2341 agacgcttgc gagaccgttg cgcgggcgcg ggccctatgg gcgctgagcc ccttctggcg
     2401 ggcgccggtc gggcgcgacc gcaggccatg cctgccggac gcaggcggcc cgtttggcgg
     2461 gggcaggctg tgcggctgcc cgttcaggct cgaacgtcat ggcgcaggag gacgccacca
     2521 tgctgagcat ccccgagaaa ctgctgttcg tcctgctcgc cgctacctcg gcgtacctcg
     2581 cctacctcgg cttccggcgg gtcgtgcagg tcatcgctcg cggccagacc ggacaggtgc
     2641 cgcgtctgaa ccagctgccg cgccgcgtct tcgacgggat catgcgcacg ctcgggcaga
     2701 cgacggtctt ccgcgcccgc cccgtggcga gcttcttcca ctccttcatc ttctacgggt
     2761 tcatcctcta cctgctcgtc aacgtcgtgg acgccatcaa cggcctactg ccgccgagct
     2821 tcaccgcccg cttcgggttc ggggtcgtcg gcgacctgta ccgcctcgtg gccgacgtcc
     2881 tcacgctcct tatcctcgtc gggatggtct acttcctcgt caggcgcttc ctcgccaagc
     2941 ccaagaccat ggagtatggg gcgagggtga agctccacga ggacgtgagg gcaggggggg
     3001 tcaagcgcga cagcctgata gtcggtagct tcatccttct gcacgtcggt tcgcggctct
     3061 tggccgagag cttcctgctg gcgagccacg gccactccga cccttaccag ccgatggcca
     3121 gcg
//
LOCUS       DAOQYE010000125         3118 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_33171_length_3118_cov_3.292524, whole genome shotgun sequence.
ACCESSION   DAOQYE010000125 DAOQYE010000000
VERSION     DAOQYE010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 3118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3118
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33171_length_3118_cov_3.292524"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1217)
                     /locus_tag="PKN52_03080"
     CDS             complement(<1..1217)
                     /locus_tag="PKN52_03080"
                     /inference="COORDINATES: protein motif:HMM:NF013591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HNQ98956.1"
                     /translation="MATSLPDRKDVDPAFTWNLQAAYETEAAFEADLASADQDLTRLT
                     GFEGRLGEGATVVADFFDAYWPTLEKLQRLRIYATMPLAVDQNDQEGRARAGRFQALA
                     ARFSTDLAFVQPELLALGSERLAGFQAQEPRLGDLKRYFERLEEGRPPVRSAEVEDVI
                     GRAADPASAFERAYNSLANGELPFKAVEHDGQTYEVARSTYPSLIASPDRALREKAYD
                     SYTRAFLGHKDTLTDLYVGRIKHSVFTARVRGFEDTVAEQLQPREVPREVLTNVLDVF
                     QKNLGVWHRYWEARRKLLGVEKLAEWDVFAPLADTPPLPYAQAIEHVLAGMAPLGDEY
                     LTPLRKGLLTERWVDVYPNRGKRDGAFATRFYRGQSYIMMSYQNNLESMSTLAHELGH
                     VMHSRLMDERQPLA"
     gene            complement(1352..1822)
                     /locus_tag="PKN52_03085"
     CDS             complement(1352..1822)
                     /locus_tag="PKN52_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98957.1"
                     /translation="MNGTTDALWLILLVVSGGVWLGGYLTMPVVARAASATLAPLDRV
                     AFFRALGRAYLPVGGLALVLALLSGAVLLREHQSGALLVATAVTAAALVAALAFGVVQ
                     ARRMTRLRQAAGVTPSDTVLSERVRRGAVAAVILRGLIGLLSLALVVMGCLLLG"
     gene            complement(2053..2667)
                     /locus_tag="PKN52_03090"
     CDS             complement(2053..2667)
                     /locus_tag="PKN52_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98958.1"
                     /translation="MRKRYLIVALGALALVAGAMALAQSTTEASAQGSLAELRQRAQR
                     LTLLAQVPADARADAEALLSRADTLRASAQDLEVERLQAYIAALEAGDSPAVATESAA
                     AQISAKKLELTRQQEALRADVEAFLADHPEAASVLRSRSNLLGAGRGDGPAFGSGVRG
                     FGGFRGLMGASDDGSGASQTPPSLQGRPQGQSRQHGPQGEGGRR"
BASE COUNT          471 a         1045 c         1122 g          480 t
ORIGIN      
        1 gccagcggct ggcgctcgtc catcagccgg gagtgcatga cgtgcccgag ctcgtgcgcc
       61 agggtgctca tgctctccag gttgttctgg tagctcatca tgatgtagga ctgcccgcgg
      121 tagaagcgcg tggcgaacgc cccgtcgcgc ttgcccctgt tcgggtacac gtcgacccag
      181 cgctccgtta gcaggccctt gcgtagcggc gtcaggtact cgtcaccaag cggcgccatg
      241 ccggccagca cgtgctcgat cgcctgcgcg tacggcagcg gcggggtgtc ggcgaggggc
      301 gcgaacacgt cccactccgc cagcttctcg acaccaagga gcttgcgccg cgcctcccag
      361 tagcggtgcc ataccccgag gttcttctgg aagacgtcca gcacgttggt caggacctcg
      421 cgcggcacct cgcgcggctg aagctgctcc gcgacggtgt cctcgaagcc gcgcacgcga
      481 gccgtgaaga cggagtgctt gatgcgcccc acgtacaggt cggtgagggt gtccttatgg
      541 ccgaggaagg cgcgcgtgta cgagtcgtac gccttctccc tcagcgccct gtcgggcgac
      601 gcgatgaggg aggggtaagt gctgcgcgcc acctcatagg tctggccatc gtgctccacc
      661 gccttgaacg gcagctcgcc gttcgcgagc gagttgtagg cgcgctcgaa ggcactcgcc
      721 gggtcggcgg cgcggccgat gacgtcctcg acctcggcgc tgcgcacggg cgggcgaccc
      781 tcctcgagcc gctcgaagta gcgcttgagg tcgccgagcc gaggctcctg cgcctggaac
      841 ccggccagcc gttcggagcc gagcgcgagg agctccggtt gcacgaacgc gaggtccgtg
      901 ctgaagcgcg ccgctagggc ctggaagcgc ccggcgcgag cgcgcccttc ctggtcgttc
      961 tgatcgacgg ccagcggcat ggtggcgtag atgcgcagcc gctgcagctt ctccagggtg
     1021 ggccagtagg cgtcgaagaa gtcggccacg accgtggcgc cttcgcctag ccggccctcg
     1081 aagccggtga gacgggtcaa gtcctggtcg gcgctggcga ggtctgcctc gaaggccgcc
     1141 tcggtctcgt acgcggcctg gaggttccat gtgaacgcgg ggtctacatc cttgcggtca
     1201 ggaagggacg ttgccatgcg tgagtttaag acgcggtgga acgcggcgac ccggcgtggc
     1261 gccgatcggg gttcggcgac gccgggcccg gcaccgcaag gcgttcgcgg ggcgctcgac
     1321 ttagccgagc agtgcttaag cccaccgcga cttagccgag cagcaggcaa cccatcacga
     1381 cgagcgcgag gctgagcaga ccgattaggc ccctgaggat aacggcggcc acggcgccgc
     1441 ggcggacgcg ctcgctcaga acggtatccg atggggtaac cccggccgcc tgccgcaaac
     1501 gtgtcatgcg ccgcgcctgt acgacgccga acgccagcgc cgcgaccagg gcggcggcgg
     1561 tgaccgcggt ggctacgagg agcgcaccgg actggtgctc acgcaggagc accgcgccgc
     1621 tgaggagcgc gagcacgagt gccaaccctc cgaccggcag gtacgcacga cccaacgcgc
     1681 gaaagaacgc cacgcggtcc agcggggcga gcgtggcgct ggcggccctc gccaccaccg
     1741 gcatggtcag gtagccgccg agccagacgc cgcccgacac gacgaggagg atcagccaga
     1801 gggcgtcggt cgttccattc atatcggtga cgggtgcgcc cagcgcgccc tagctcggag
     1861 gtccatgctg gcaggttgca tgacgggagc gtaaccgagg gtgcgcacgt tctgggcggc
     1921 tgcagggacg gaaggccaga tccgtggaag ggtcgccgct acctcgtgtg tagcgacgac
     1981 ctcctggtgt cgtgtgtcct agtctcttgc tgtcctagtc tcttgtgtcg gttcgggcat
     2041 cttcgtcagc tgctagcgac gacctccttc tccttgcggg ccatgctgtc gcgactggcc
     2101 ctgcggccga ccctggaggg acggcggcgt ttggctggct ccactgccgt cgtcagatgc
     2161 gcccataagg cccctgaagc cgccgaaccc gcgcactccg ctgccgaacg caggcccgtc
     2221 gccccgtcct gcaccgagca ggttggagcg tgagcgcagg acgctcgccg cctcgggatg
     2281 gtcggccagg aaggcctcga cgtcggcgcg cagcgcctcc tgctggcgcg tcagctcgag
     2341 cttcttggcg ctgatctgcg cggctgccga ctcggtggcg acggccggcg aatcgccggc
     2401 ctcgagcgcg gcgatgtacg cctgcaggcg ttcgacctcg aggtcctgcg cactggcgcg
     2461 cagcgtgtca gcgcggctca gcagcgcctc ggcgtccgcg cgcgcgtccg ccggcacctg
     2521 cgctagcagg gtgaggcgct gggcccgctg gcgcagttcc gcaaggctgc cctgtgcgct
     2581 cgcttccgtg gtcgactgcg ccaaggccat ggcgccggcc accagcgcca aagcgccgag
     2641 cgcgacgatc aggtaacgct tcctcatgct ctctccttta cgtaggtcgg ggcccgcgct
     2701 ggggctatcc ccgacctggc cgcaggctag gggcgacgtg tgcagactcc gaggagggaa
     2761 gaggtaggga aacggtggcc cgcgcaaccg cgcatcttgg gtcggatcgg ggtgcgaccg
     2821 ggaggaggga ggggagggaa acggtggctt atgcaacgtg gacgttgcac gaggacggtc
     2881 tggcggcgat gagtgcgacg cggcaacctt gagcctgccg ctggccttca ccggaactac
     2941 acagagcgcc actagagtct tgaggcatgg gcgagaccga cgtcaccggc accagggtcc
     3001 tgatcgtcga ggacgaacgc ggcatctctc gcaccctcga gagctacatg aagcgtgagg
     3061 ggatggtcac ggaggtggcg gcctcgggct ccgaggccct gcgcctcttc cgcgcggc
//
LOCUS       DAOQYE010000126         3116 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_33197_length_3116_cov_3.070238, whole genome shotgun sequence.
ACCESSION   DAOQYE010000126 DAOQYE010000000
VERSION     DAOQYE010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 3116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3116
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33197_length_3116_cov_3.070238"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..819)
                     /locus_tag="PKN52_03095"
     CDS             complement(<1..819)
                     /locus_tag="PKN52_03095"
                     /inference="COORDINATES: protein motif:HMM:NF018613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98959.1"
                     /translation="MTDPPRVLILSASVGGGHVAAGKALHTAIVAQGAHAEHIDLLEY
                     SSMPFRRLYSQAYLDLARTMPDLVEWIGRRFDRNPSEYKGVQQRLRARVTRLLSYEVP
                     GLIDRYRPDALVHTHFLGAEIVSGRMRRRRPLPQAEVITDFFAHAFWLQPGIARYFVA
                     SDDVRAQLMAAGVDDHRIRVTGIPIDPRFAALPDKAAARTALGLGQDSDVLLLLANGL
                     GEDKLREVMEQLIALQSPLQVVVVCGRSQELYERSRALAGGAEAPVTVRVLGLTT"
     gene            952..2025
                     /locus_tag="PKN52_03100"
     CDS             952..2025
                     /locus_tag="PKN52_03100"
                     /inference="COORDINATES: protein motif:HMM:NF025701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease complex subunit PrcB family protein"
                     /protein_id="HNQ98960.1"
                     /translation="MPRIWLLLAVIGVTLAGCVPTSALRMDDLYIYGIENARLTYFYG
                     EAGQILYDGGSLGLAEQPESAVPTGNYVVRGALAAGKPFLRVAVDPLPVEPVVVSRIP
                     FTTDLQVALKTDVDEVVYYDGQSFLRLLETDEAGTVRPVVPRPRLNGLRGLGQLTNAE
                     ADALAAALTAGGRPFALAALPIASLPSHAVDGLSEHRRTGIYVQREIATDASAYRPTP
                     ERLTWDVVARGDQAVGFASASYQLVTSQDELISLWQRAYGSRLTVPALPSLDFRRETI
                     IALFMGSRSSGGYSMDVTDVREEGGELYVELQVTEPAPGTITTQALTSPWLILRVQRG
                     GYAAAWLRDTGSGNLLGVARADR"
     gene            complement(2076..>3116)
                     /locus_tag="PKN52_03105"
     CDS             complement(2076..>3116)
                     /locus_tag="PKN52_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HNQ98961.1"
                     /translation="AYRTAFQKDRDRVVHTSAFRRLEYKTQVFVNYEGDYYRTRLTHT
                     LEVAQVATSIARALGLNEDLAETIALAHDLGHPPFGHAGEKSLNELAANVGGFDHNRQ
                     SLRIVTKLERRYPGFPGLNLTWETLEGIMKHETEYDLPDPSWEPDVQPTLEAQVVNAA
                     DEVAYNAHDLDDGLRSGHLKPTAVATVPIIGELMTRLGLNAERFDSSQRYVLIRELLG
                     DVITDVVHATDARLTDHGVRSLDDVRRAGKKLLGPSEAMGRQLAELKEFLYANFYFHH
                     RLIRMTHKAHLVLKSIYHAYVETPDMLPPDVVSQVQALGLERAVTDYIAGMTDRYAND
                     EYRRLFDPTALT"
BASE COUNT          470 a         1016 c         1115 g          515 t
ORIGIN      
        1 ggtcgtgagc ccgagcaccc tgacggtgac gggagcttcc gcgccgccgg ccagtgccct
       61 cgagcgctcg tagagctcct ggctgcggcc gcatacgacc acgacctgca ggggcgattg
      121 cagcgctatc agctgctcca tgacctcgcg cagcttgtcc tcgccgaggc cgttcgccag
      181 taggaggagg acatcgctgt cctggccgag gccgagggcg gtgcgcgccg ccgctttgtc
      241 cggcagggcg gcgaagcggg ggtcgatcgg tatgccggtg acgcggatcc tatggtcgtc
      301 cactccggcg gccatgagct gcgcgcgcac gtcgtcgctg gccacgaagt agcgcgctat
      361 gccgggctgc aaccagaagg cgtgggcgaa gaagtccgtg atgacctcgg cctgcggcag
      421 cggccgcctg cgccgcatgc gccctgacac gatctcggcg ccgaggaagt gcgtgtgtac
      481 tagagcgtcg gggcggtaac ggtcgatgag gccgggcacc tcgtacgaga gcaggcgcgt
      541 gacgcgcgcc cgcaggcgct gctgcacacc cttgtactcg ctcgggttgc gatcgaagcg
      601 cctgccgatc cactcgacca gatcgggcat ggtgcgagcg aggtccaggt acgcctggct
      661 gtagagccgt ctgaacggca tggacgagta ctcgagcagg tcgatgtgct cggcgtgcgc
      721 cccttgggcg acgatggccg tatgcaacgc cttgccggcg gctacgtggc cgccgcctac
      781 cgaggcggac aggatcagca cccgtggcgg gtcggtcact gccggagcat agcaagcgag
      841 cctgagcgct tgaacagggg cgggggaggc ttcccggcac cgcggcccac ccgcgggtgc
      901 gtggagagat tctcaagaag ccggtgatag catgcgacgt ggagggtacg aatgccacga
      961 atctggctgc tgctcgccgt catcggcgta actctcgcgg ggtgtgtgcc gacctcggcg
     1021 cttaggatgg acgaccttta catctacggg atcgagaacg cccggctcac ctacttctac
     1081 ggcgaagcgg gtcagatcct gtacgacggg ggaagcctgg gcctcgccga acagccggaa
     1141 tccgcggtcc cgaccgggaa ctacgtcgta cggggcgcgc tcgcggccgg caagccgttc
     1201 ctgcgcgtgg cggtcgaccc gctccccgtc gagcccgtcg tggtcagccg catcccgttc
     1261 accacggacc tgcaggtcgc cctcaagacc gacgtggacg aagtggtgta ctacgatggc
     1321 cagtcgttcc tccgcctgct cgagaccgat gaggctggca cggtgcgtcc ggtcgtgcca
     1381 cgaccgcgct tgaacgggct gcgtggactc ggccagctca ccaacgccga ggccgatgcc
     1441 ctggccgccg cgctgaccgc cggcggtcga ccgttcgcgc tcgcggcgct gcccatcgcc
     1501 agcctgccgt ctcacgccgt cgacggcctc tccgagcacc gccgcacggg catctacgta
     1561 cagcgcgaga tagccacgga cgcttccgcc taccggccga cacccgagcg cctgacatgg
     1621 gacgtcgttg ctcgcggcga ccaggcggta gggttcgcct ccgccagcta ccagctcgtc
     1681 accagccagg acgagctcat atccctctgg cagcgcgctt acggcagccg cttgacggtg
     1741 ccggcgctgc caagcctcga tttccggcgc gagacgatca tcgccctctt catgggcagc
     1801 cgctcgtccg gcggctactc catggacgtc acggacgtgc gggaggaggg cggcgagctc
     1861 tacgttgagc tgcaggtcac ggaaccggcc ccgggcacca tcacgaccca agcgctcacg
     1921 agcccgtggc tgatcttgcg cgtgcagcgc ggcggttacg ccgctgcgtg gttgcgcgac
     1981 acgggttcgg gcaacctgct cggggtggcg cgcgccgacc gctgacagtc caacgtggcg
     2041 gcgcaccgac ggtgcgccgc cacgagccgg ctacatcagg tgagcgccgt cggatcgaag
     2101 aggcgacggt actcgtcgtt cgcgtagcgg tccgtcatgc cggcgatgta atcggtgacg
     2161 gcccgttcga ggccgagcgc ctgcacctgg ctcacgacgt cgggcgggag catgtcgggc
     2221 gtctcgacgt acgcgtggta gatgctcttc aacaccagat gcgccttgtg ggtcatccgg
     2281 atcagccgat ggtggaagta gaagttcgcg tagaggaact ccttgagctc ggctagctgc
     2341 ctgcccatgg cctcggaggg cccaaggagc ttcttgccgg cgcggcgcac gtcgtcaaga
     2401 ctcctgacac catggtcggt caggcgcgcg tccgtggcgt gaacgacgtc ggtgatgacg
     2461 tcgccgagga gctcgcggat gagcacgtag cgttgcgagg agtcgaagcg ctcggcgttg
     2521 agaccgaggc gcgtcatcaa ctcgccgatg atagggactg tggcgacggc cgtcggcttc
     2581 aggtgaccgg agcgcagccc gtcgtcgagg tcgtgggcgt tgtaggcgac ctcgtctgca
     2641 gcgttgacga cctgggcctc gagggtcggc tgcacgtctg gctcccagct cgggtcgggg
     2701 aggtcgtact ccgtctcgtg cttcatgatc ccctcgagcg tctcccacgt gaggttcagg
     2761 ccggggaacc cagggtagcg gcgctcgagc ttcgtcacga tgcgtaggct ctgccggttg
     2821 tggtcgaagc cgccgacgtt ggcagccagc tcgttgagcg acttctcgcc ggcgtggccg
     2881 aacggcggat ggccgaggtc gtgcgcgagg gcgatggtct cggcaaggtc ctcgttgagg
     2941 ccgagggcgc gcgcgatgct cgtggcgacc tgcgcgacct cgagcgtgtg ggtgaggcgg
     3001 gtgcggtagt agtcgccctc gtagttgacg aacacctggg tcttgtactc gagtcgcctg
     3061 aaggcgctcg tgtgcacgac gcggtcgcgg tccttctgga aggccgtgcg ataggc
//
LOCUS       DAOQYE010000127         3114 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_33232_length_3114_cov_2.625695, whole genome shotgun sequence.
ACCESSION   DAOQYE010000127 DAOQYE010000000
VERSION     DAOQYE010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 3114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3114
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33232_length_3114_cov_2.625695"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..754)
                     /locus_tag="PKN52_03110"
     CDS             complement(<1..754)
                     /locus_tag="PKN52_03110"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HNQ98962.1"
                     /translation="MRERHVSELIDEVFRRAAVKRGVRRAEAVLAWRRVVGAELAAFS
                     SAVALRDGVLYVNVGDSETAMHLSLGRQRIVDAYRATLGTAAVRDIRFQVGRLDSAES
                     SGSPAPALPSPDSEALAALTAELARLDLPDELAQAARSAGARLLGLQAARLALGWTVC
                     PTCGALHDGPVRPETLREAALKAAGRRDQDAELERALCQACYRYAHEGRVVAAAQRLR
                     TSPQAPTEGLGEDERAVATYLATRSLDSVIAAL"
     gene            complement(747..1805)
                     /gene="recF"
                     /locus_tag="PKN52_03115"
     CDS             complement(747..1805)
                     /gene="recF"
                     /locus_tag="PKN52_03115"
                     /inference="COORDINATES: protein motif:HMM:NF010680.0"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HNQ98963.1"
                     /translation="MRLVSLTQLNFRNLRTPRIDFPSGVVAVVGHNAAGKSNLLAAAY
                     LACTGETVGGKLARQVRLGEDEAYVAAEIEHDEGVSRVEVGLGAGKKVVRVDGQAAKV
                     GDVARVVSAVLLTPEDSDLVHGPPASRRAYLDDLLAKLSLRYALLHREYGRVVEHRNA
                     LLRAGRFGAELTPWSERFVALGTEVLALRERAVARLAPLAAEVYRDVAGAGSLAVRLG
                     TTHGESTLGEALAAGAEAERLRGQTLIGPHRDDLVLALDEVSLPEYGSRGEARTAALA
                     LRVAEYRLLSEKHREPPVLLIDDFTAELDATRRAYLLELAGATPQALVSGTEPPPLAD
                     ARIEMAAGHARLLEASRA"
     gene            1967..>3114
                     /locus_tag="PKN52_03120"
     CDS             1967..>3114
                     /locus_tag="PKN52_03120"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufS family cysteine desulfurase"
                     /protein_id="HNQ98964.1"
                     /translation="MDDLDVLALRRDFPILSRQVNGHPLTYLDSAASSQRPRQVVAAM
                     QEYYERHHANVHRGAHTLATEATDAFEGARTRVARFIGAPEARSLVFTRNTTEAINLV
                     AASWGRSNLGAGDEVVVSVAEHHANLVPWHFLKRERGIVIRPVPLTVDHRLDLDALRA
                     AIGPRTKLVSTFHMSNVLGAINPVAEIAGLAHAAGALLLVDGAQAAPHLVVDVAALGC
                     DFYAFSGHKMLGPTGIGALWARPEVLEAMPPFLGGGEMIRRVTLEDSTYADIPARFEA
                     GTPSIGEAVGLAAAVDYLSAVGMDRVARHDRELASYALDLLSDVPGLTLFGPRGDDRG
                     GIVAFDAAGIHAHDVATMLDLEGIAVRAGHHCAQPLGAESGSSATALVA"
BASE COUNT          425 a         1146 c         1128 g          415 t
ORIGIN      
        1 gcagcgcggc gatgacggag tcgagcgagc gcgtagccag ataggtggcg acggcgcgct
       61 cgtcctcgcc aaggccttcg gtcggcgcct gcggggacgt gcgcaggcgc tgcgccgcgg
      121 ctaccacccg cccctcgtgg gcgtaccggt agcacgcttg gcagagggcg cgctccagtt
      181 cggcgtcctg gtcgcgccgg ccggccgcct tgagggcggc ttcccgcagc gtctcagggc
      241 ggacgggccc gtcgtgcagg gcgccgcagg tcgggcagac cgtccagccg agggcgagcc
      301 gggccgcctg caggccgagg agcctcgcgc cagccgagcg ggccgcctga gccaactcgt
      361 ccggcagatc aagcctcgcg agttccgctg tcagcgcggc gagcgcctcc gagtcggggc
      421 tcggcaacgc cggcgccggg gagccggacg actcggcgct gtccaggcgt cctacctgga
      481 agcggatgtc gcgaacggct gcggtgccga gggtggcgcg ataggcgtcc acgatgcgct
      541 ggcggccgag gctcaagtgc atggccgtct ccgagtcgcc gacgttgacg tacaggacgc
      601 cgtcccggag cgccacagcg gagctgaacg ccgccagttc cgccccgacg accctacgcc
      661 aggccagcac cgcctcggcg cgccgcacgc cgcgcttgac ggccgcacgg cggaaaacct
      721 cgtcgatcag ttccgagacg tgccgctcac gcacgactgg cctccaggag gcgcgcgtgg
      781 cccgccgcca tctcgatgcg cgcgtcggcc aacgggggcg gctccgtgcc ggacacgagg
      841 gcctgcggcg tggcgcccgc cagctccagg aggtaggcgc gtcgcgtcgc gtcgagctcg
      901 gccgtgaagt cgtcgatgag cagcacgggc ggctcgcggt gcttctcgct caagaggcga
      961 tactccgcga cccgcagcgc gagcgcggcc gtgcgggcct cgccgcgcga gccgtactct
     1021 ggcaacgaca cctcgtcgag agcgagcacc aggtcgtcgc ggtgcgggcc gatcagagtc
     1081 tggcccctga ggcgctcggc ctccgcgccc gcggccaagg cttcgcccaa ggtcgactcc
     1141 ccatgcgtgg tgccgaggcg gacggcgagc gagccggccc cggccacgtc gcggtagacc
     1201 tcggctgcga gcggggcgag acgcgccacg gcgcgctccc gcagggcgag gacctccgtg
     1261 ccgagcgcga cgaagcgctc gctccacggt gtcagctccg ccccgaaacg tccggcgcgc
     1321 aggagcgcgt tgcggtgctc gacgacgcga ccgtattcgc ggtgcaggag cgcgtagcgc
     1381 agtgacaact tggctagcag gtcgtcaagg taggcgcgtc gtgaggccgg tggcccgtgc
     1441 acgaggtcgc tgtcctccgg cgtgaggagg acggcgctga cgacgcgggc cacgtcgccg
     1501 accttggcgg cctggccgtc gaccctcacc accttcttgc cggcccctag cccgacctcg
     1561 acgcgcgaca cgccctcgtc gtgctcgatc tccgcggcca cgtacgcctc gtcctcgccg
     1621 agcctgacct gccgtgcgag cttgccgccg acggtctcac ccgtgcaggc caggtacgcg
     1681 gccgccaaca ggttgctctt gccggccgcg ttgtgtccga cgaccgctac caccccgctc
     1741 gggaagtcga tgcgcggcgt gcggaggttg cggaagttga gctgcgtcag gctgacgagc
     1801 cgcacacgcc gagtctaacg ccctgccggc cacgcccccg tcggcgcgcc ggctcggcgc
     1861 gcgggctcgg cgcggcgtgc cggcacgccg tgctgcccgc gccgtaccgc cgggtgagtc
     1921 actgcggggc gttcggcgcg ttaggccggt gctagactcg ccgctgatgg acgacctcga
     1981 cgtcttggcg ctcagacgcg actttcccat cttgagccgc caggtgaacg gccacccact
     2041 gacgtacctg gacagcgcgg cgtcgagcca acggccgcgg caggtcgtgg ccgccatgca
     2101 ggagtactac gagcgccacc acgccaacgt ccaccggggc gctcacacgc tcgccacgga
     2161 ggctaccgac gccttcgagg gcgcgcggac gcgcgtcgcg cgcttcatcg gcgctccgga
     2221 ggcgcgttcg ctcgtgttca cacgcaacac cacggaggcc atcaacctgg tcgccgcgag
     2281 ctgggggcgc tccaacctcg gcgcgggcga cgaggtggtc gtcagcgtcg ccgagcacca
     2341 tgcgaaccta gtgccttggc acttcctgaa gcgcgagcgc ggcatcgtca tccgccccgt
     2401 gccgctcacg gtcgaccacc gactcgatct agacgcgctg cgcgcggcca tcgggccacg
     2461 cacgaagctc gtgagcacct tccacatgag caacgtcctt ggggccatca accccgttgc
     2521 ggagatcgcc gggctcgcgc acgccgccgg ggcgctcctc ctcgtcgacg gggcgcaggc
     2581 cgcgccccac ctggtggtcg atgtggccgc gctgggctgc gacttctacg ccttctccgg
     2641 ccacaagatg ctcggaccta ccggcatagg ggccctctgg gcccgtccgg aggtgctcga
     2701 ggccatgccc cccttcctgg ggggcggcga gatgatcagg cgcgtgacgc tggaggacag
     2761 cacctacgcc gacatcccgg cgcgcttcga ggccggaacg cccagcatcg gggaggcggt
     2821 cgggctcgcg gcggccgtcg actacctgag cgcagtgggg atggacaggg tggcgcgtca
     2881 cgaccgcgag ttggcgagct acgcgctcga cctgctgagc gacgtgccgg gcctgacgct
     2941 cttcggaccg aggggcgacg accgcggcgg catcgtcgcc ttcgacgccg ccggcatcca
     3001 cgctcacgac gtggccacga tgctcgacct ggaggggatc gcggtgcgcg cggggcatca
     3061 ctgcgcccaa ccgctcgggg cggaaagcgg atcttccgcg accgcattgg tcgc
//
LOCUS       DAOQYE010000128         3112 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_33282_length_3112_cov_2.960746, whole genome shotgun sequence.
ACCESSION   DAOQYE010000128 DAOQYE010000000
VERSION     DAOQYE010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33282_length_3112_cov_2.960746"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1604
                     /locus_tag="PKN52_03125"
     CDS             <1..1604
                     /locus_tag="PKN52_03125"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HNQ98965.1"
                     /translation="LAGLILGLAWSLWRRQPVVRVVLTTIAGGLAGLLIGLMLFARGA
                     VPSSLALAVIAATAATLLGLGFALLGQRTRFRFVKASLGAFSVLPIVTPPFILAFSMI
                     FLLGRRGLLTFDILGISSNWIFGIPGVAFAQILAFTPIAYMVIQGSVAALDAALEEAS
                     TTLGASQWHTFRTVTWPLLRPGLANAFLLTVIESFADFGNPLILGGDRNYLATEVFSA
                     FAARFDQAEAAVYGVVLLFVVLLVFYAQARWLGRSSFVTVTGKPSRRAFTRLPVGLEA
                     LLLVVYAVWAVTVVALYASIIYGSFVQLWGVNGTLTLKHWRDLTNAGVPVFLYTVRIA
                     AISAIPAALLGFLIAYLVVRQEFWGRRLLEFGSMLSFSTPGTVMGIAYILAFNTGPWL
                     LTSSAVIIVLSFVFRNMPVAIRGGVAGFAQIDKSLEEASTMLGSSSFRTLRTVLVPLL
                     IIPIVSGLIFAFVRAMTAISQVIFVVSPGNQLTTVLLLGWVEQGQTGRAAAMGTLLIV
                     SMLVVILLLNLLTRRRGGITGEVVA"
     gene            1604..2707
                     /locus_tag="PKN52_03130"
     CDS             1604..2707
                     /locus_tag="PKN52_03130"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ98966.1"
                     /translation="MPAPAPVRLEGVTKKFGQDVMAVDRLDLEITPGSLVTLLGPSGC
                     GKTTTLRMIAGLEAVSGGSILIDGQDVTRLAANHRDVTMVFQSYALFPHMSVFDNVAY
                     GLRIAKVHGADLERRVADALATVGMVGLEKRSPAALSGGQQQRVALARSLVLEPRVLL
                     FDEPLSNLDAKLRRRVRQEIRDLQQRLGITSVYVTHDQEEALAISDTIVVMQGGRIQQ
                     QGSPHELYTQPANRFVADFIGSANFLPGDYDGSSVRVGPYRFAYEQAFPKGKATVMVR
                     PEAVRLATAAEAERQAAASAGGAEPGYLPATVKAVSYLGVVSELLLDTPLGELASSVS
                     GEGRSRFLRGDNVQVTFHTAGVYLLPEGGETGA"
     gene            2704..>3112
                     /locus_tag="PKN52_03135"
     CDS             2704..>3112
                     /locus_tag="PKN52_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HNQ98967.1"
                     /translation="MSTVGPRVRLTAGQAIVKFLAAQSSERDGVRRRLVPGMFAILGH
                     GNVTGLGQALQELGPEVDMRAYRPQNEQAMVHAAAAFAKHTDRLQTLACTASIGPGST
                     NMLTGAAGATINRLPVLLFPSDYFANRIPDPVLQ"
BASE COUNT          425 a         1171 c          975 g          541 t
ORIGIN      
        1 cgcttgccgg cctgatcctc ggcctcgcct ggagcttgtg gcgtcggcag ccggtcgtgc
       61 gcgtcgtcct gaccaccatc gcgggcggcc tggccggcct gctcatcggg ttgatgctct
      121 tcgcgcgcgg cgccgtgccg agcagcctcg cgctcgccgt catcgccgcc accgcggcca
      181 ccctcctcgg gctcggcttc gccctcctcg gccaacggac gcggttcagg ttcgtcaagg
      241 cgtcgctcgg cgccttcagc gtgctgccca tcgtcacgcc gcccttcatc ctcgcgttct
      301 cgatgatctt cctcctcggg cggcgcgggc tcctgacgtt cgacatcctc ggtatctcga
      361 gcaactggat cttcggtatc cccggcgtgg cgttcgcgca gatactggcg ttcacgccca
      421 tcgcgtacat ggtcatccag ggctcggtgg ccgccctcga cgccgccctt gaggaggctt
      481 cgacgacgct cggcgccagc cagtggcaca cgttccgcac ggtgacctgg ccgctcctgc
      541 gcccaggcct cgccaacgcc tttctcctga ccgtcatcga gtctttcgcc gacttcggca
      601 acccgctcat cctcggcggc gaccgcaact acctcgccac agaggtcttc tcggcgttcg
      661 ccgcgcgctt cgaccaggcc gaggcggccg tctacggcgt cgtgctcctc ttcgtcgtgc
      721 tgctcgtgtt ctacgcgcag gcacggtggc tcggccgcag ctccttcgtg accgtgacag
      781 gcaaaccgtc tcggcgcgcc ttcacgcgcc tgccggtcgg gctcgaggcg ctcctgctcg
      841 tcgtgtacgc cgtatgggcc gtcaccgtcg tcgccctcta cgcctccatc atctacggct
      901 cgttcgtgca gctctggggc gtgaacggga cgctgaccct caagcattgg cgcgacctca
      961 cgaacgccgg cgtgccggtg ttcctataca ccgtcaggat cgcggccatc agcgccatcc
     1021 ccgcggccct cctaggcttc ctcatcgcct acttggtcgt caggcaggag ttctggggca
     1081 ggcgactact cgagttcggg tccatgctgt cgttctcgac gccgggcacc gtgatgggca
     1141 tcgcgtacat ccttgccttc aacaccggcc cctggctgct cacctcgagc gccgtgatca
     1201 tcgtgctgtc gttcgtcttc cgcaacatgc ccgtcgccat ccgcggcggc gtggcgggct
     1261 tcgcgcagat agacaagagc ctcgaggagg cttccaccat gctcggctcg agctcgttcc
     1321 gcacgctgcg taccgtcctc gtgccgctcc tcatcatccc gatcgtctct ggcctgatct
     1381 tcgcgttcgt gcgcgccatg accgcgatca gccaggtcat cttcgtcgtc agcccaggca
     1441 accagctgac gaccgtgctc ctcctcggct gggtcgagca aggccagaca gggcgcgcgg
     1501 cggcgatggg tacactgttg atagtgtcca tgctggtcgt gatcctgctg ctcaacctgc
     1561 tcacccggcg gcgcgggggc atcaccggag aggtggtggc ctgatgcctg ccccggcacc
     1621 cgtaagactc gagggcgtca ccaagaagtt cggccaggac gtcatggccg tcgatcggct
     1681 cgacctggag atcacgccag gctcgctcgt caccctgctc ggcccgtccg gctgcggcaa
     1741 gacgacgacc ctgcgcatga tcgccgggct cgaggccgtc agcggcggca gcatcctcat
     1801 cgacggccag gacgtcacgc gcctcgccgc caaccaccgc gacgtcacca tggtgttcca
     1861 gagctacgcg ctcttccccc acatgagcgt cttcgacaac gtggcctacg gcctgcgcat
     1921 cgccaaggtc cacggcgccg acctcgagcg gcgcgttgcg gatgccctgg cgaccgtcgg
     1981 catggtcggc ctcgagaagc gcagcccggc cgccctctcc ggtggccaac agcagcgcgt
     2041 cgccctggcg cgctcgctcg tgctggagcc gcgcgtcctc ctcttcgacg aacccctctc
     2101 caacctcgac gccaagctcc gcaggcgcgt gcgccaggag atccgtgacc tgcagcagcg
     2161 cctcggcatc acgagcgtct acgtcaccca cgatcaggag gaggccctcg ccatctccga
     2221 cacgatcgtg gtcatgcagg gcggccgcat ccagcagcag ggctccccgc acgagctcta
     2281 cacgcagccg gccaaccgtt tcgtggccga cttcatcggt tccgccaact tcctgccggg
     2341 cgactacgat gggtcatcgg tgcgcgtcgg cccttaccgc ttcgcgtacg agcaggcctt
     2401 ccccaagggg aaggcgaccg tgatggtcag gcccgaggcc gtgaggctcg ccacggccgc
     2461 cgaggccgag cgccaagcgg cggcaagcgc cggcggtgcc gagcccgggt acttgcccgc
     2521 caccgtgaag gccgtcagct acctcggggt ggtgagcgaa ctgctcctag acacgccgct
     2581 cggcgagctc gcctccagtg tctcgggcga ggggcgcagc cgcttcctgc gaggcgacaa
     2641 cgtccaggtc acgttccaca cggccggggt ctacctgttg ccggaggggg gagagaccgg
     2701 cgcatgagca cggttgggcc tagggtgcgg ctgaccgccg ggcaggcgat cgtcaagttc
     2761 ctcgccgcgc agtcctcgga acgcgacggg gtccgcaggc ggctcgtgcc tgggatgttc
     2821 gccatcctcg ggcacggcaa cgtgaccggt ctcggccagg ccctgcagga gctcgggcct
     2881 gaggtcgaca tgcgcgcata ccggccccag aacgagcagg ccatggtgca cgcggccgcc
     2941 gcgttcgcga agcacaccga caggctgcag acgctcgcgt gtacggcctc catcggcccc
     3001 ggctcgacga acatgctcac gggcgcggcc ggcgcgacca tcaaccgcct gcccgtcctc
     3061 ctcttcccgt ccgactactt cgccaaccgc atccccgacc ccgtgctgca gc
//
LOCUS       DAOQYE010000129         3099 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_33480_length_3099_cov_3.144218, whole genome shotgun sequence.
ACCESSION   DAOQYE010000129 DAOQYE010000000
VERSION     DAOQYE010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 3099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3099
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33480_length_3099_cov_3.144218"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(77..901)
                     /locus_tag="PKN52_03140"
     CDS             complement(77..901)
                     /locus_tag="PKN52_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ98968.1"
                     /translation="MVRRRIDVAKRANLIIGAALVGTVALAALLSFMWLPTDPLKMNF
                     ASQLKPPSGGFPLGTDHFGRDLLSRVLVGARSTLYVGFIAVGIALTAGSLLGALAGFL
                     GGVVDEVIMRLIDVAYAFPAILMAMLLAAVYKPGTLTAMVAIGIASVPIFARIARSSV
                     LSLKEREYVEAGRALGVRPGGCGGAGACPSAMGPLIVQASLSLAVAILAEAALSFLGL
                     GTPPDVPSWGNMLREAQGFMAMSPYPALVPGVAIVWTVLGWSLLGDGLRDLWDPRG"
     gene            complement(901..1845)
                     /locus_tag="PKN52_03145"
     CDS             complement(901..1845)
                     /locus_tag="PKN52_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ98969.1"
                     /translation="MLAFTVRRFFTAVLSVWLASLVVFSAIIAVPGDPAEIILGLNSS
                     AEARAALRTKLGLDVPAPRRYVAWLGNVLRGDFGESLNYQQPVGRLIKDRFKVSVPLA
                     LGAALVATLLAIPLGIVAAMRRGTFVDPLVMTLSQVGAAIPSFWLGLIFILYFSVRLR
                     LFPAGGFTPFDTDPVKWFRSLVLPVVALGLGQAAVMMRMTRAAMLEVLSQDFVRTARA
                     KGVGTRSVVLKHALRNAMVTIITILGLSLTNVFIGSIIVEQVFALPGLGRLALTAIGT
                     RDFPLLQGEILVYAATIVFLGFVVDLSYGLLDPRIRYS"
     gene            complement(2061..>3099)
                     /locus_tag="PKN52_03150"
     CDS             complement(2061..>3099)
                     /locus_tag="PKN52_03150"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNQ98970.1"
                     /translation="PSRGLLYDLARPDSIIYPAAKADTQRSQPIGTGPFKFASYTEGS
                     EVVLERNPDYYVAGVPYLDKAVFKIISDANTRFAALQAGDIVLTGVTSEQYLVLGGSP
                     DLKGTEGTMTTEITVAMNNTRPPFDDVRVRQAITMAIDKQAIVQGAMFGLGTVIGSHM
                     SPSEPYYIDLSNTYPYDPQRAKELLAEAGYADGLTIHFELPEPYVIERRSGEVVAQML
                     RDIGINVELSVVEWGTWIQRIFLGGDYDMTIIGHSEPRDINVYANPGYYYHYDNPRIG
                     ELLGEAEAADSTAAETAAYQEIAKIIANDAVNVWLFSPPYLVAARQNVYGFWQDQPTP
                     AIDVTEVFKTN"
BASE COUNT          508 a          978 c         1139 g          474 t
ORIGIN      
        1 gaagccctgg gaccggatca gaggaagcga gcgtgccgcg gcggtgggcg gagcgacgcc
       61 gtgctccgcg ccctcgtcac ccgcgcgggt cccacaggtc cctgagcccg tcgccgagga
      121 ggctccagcc gagcaccgtc cagacgatgg cgacgccggg tactagcgcc gggtaggggc
      181 tcatggccat gaagccctgc gcctcgcgca gcatgttccc ccagctgggc acgtccggtg
      241 gggtaccgag gccgaggaac gagagcgcgg cctccgccag gatggcgacg gcgaggctga
      301 ggctcgcctg gacgatcagc ggccccatgg cgctcgggca ggctcctgct ccgccacatc
      361 ctcccgggcg cacgccgagt gcgcggccag cctcgacgta ctcgcgctcc ttgaggctca
      421 gcacgctcga ccttgcgatc cgcgcgaaga tgggcacaga agctatcccg atggcgacca
      481 tggcggtcaa cgtgcccggc ttgtacacgg ccgccaagag catcgccatg aggatggccg
      541 ggaaggcgta cgccacgtcg atgagccgca tgatcacctc gtcgaccacg ccgccgagga
      601 agcccgccag ggcgccaagg aggctcccgg cggtaagggc gatgccgacc gcgatgaaac
      661 cgacgtagag cgtggagcgc gcccccacca gcacgcgcga gagcaggtcg cggccgaagt
      721 ggtctgtgcc gagcgggaag cccccagagg gcggcttcag ctgactggcg aagttcatct
      781 tgagagggtc ggtaggcaac cacatgaagc tgagcagcgc ggcgagggct acggtcccga
      841 cgagcgctgc cccgatgatc aggttcgccc gcttggcgac gtcgatccgt ctgcggacca
      901 tcaggagtac cggatgcgcg ggtcgaggag cccgtacgag aggtcgacga cgaacccgag
      961 gaagacgatg gtggccgcgt acacgaggat ctcaccttgg aggagcggga agtcgcgcgt
     1021 gccgatggcc gtcagcgcca gccgccccag cccgggcagc gcgaacacct gctcgacgat
     1081 gatggagccg atgaagacgt tggtcaggga gaggcccagg atcgtgatga tcgtgaccat
     1141 ggcgttgcgc agggcatgct tgagcacgac gctgcgcgtg ccgaccccct tggccctggc
     1201 ggtgcgcacg aagtcctggc tcaggacctc gagcatggcc gcgcgggtca tgcgcatcat
     1261 caccgcggcc tggcccagcc cgagcgccac gacgggtagc acgaggctcc tgaaccactt
     1321 caccgggtcg gtgtcgaacg gcgtgaagcc gcccgccggg aagagcctca gtcgcacgga
     1381 gaaatacagg atgaagatga ggccaagcca gaagctcggg atggcggcgc cgacctgcga
     1441 cagcgtcatc acgagcgggt ccacgaacgt gccgcgccgc atggcggcga cgatccccag
     1501 gggtatcgcg agcaacgtcg cgaccagggc ggcgcccagt gccaagggca cgctcacctt
     1561 gaaccggtcc ttgatgagcc ggccgacggg ctgctggtag ttgaggctct cgccgaagtc
     1621 gccgcgcagc acgttcccga gccaggcgac gtagcgtcgc ggggcgggaa cgtcgaggcc
     1681 cagcttggtg cgcagggccg cgcgcgcctc ggcgctcgag ttgaggccga ggatgatctc
     1741 ggcgggatcg ccggggaccg cgatgatggc ggagaagacc acgaggctgg ccagccatac
     1801 gctcaggacg gcggtgaaga agcgcctgac tgtgaaggcc agcatccggc gttaccaagg
     1861 tcccttcgcg gtcggttgcg cgtccgcccg gtagttcgag cccgccgcgg tcgttcgcgg
     1921 gcgggcttgg cttctggggc ggcgccgtgg tcggcggcgc cccaggactg gcgatgatcg
     1981 ggcgcggctg aagcgagccg gtcggctccg agcgagccgt tcgaacggct atggtctacc
     2041 ggctcgctcg gagcccgcgc tcagttcgtc ttgaacacct cggtcacgtc gatggccggc
     2101 gtgggctggt cctgccagaa gccgtagacg ttctgcctgg ccgcgacgag gtacgggggc
     2161 gagaacagcc acacgttcac cgcatcgttc gcgatgatct tggcgatctc ctggtaggcg
     2221 gccgtctcgg cggccgtgct gtcggccgct tcggcctcgc cgagcagctc gccgatgcgc
     2281 gggttgtcgt agtggtagta gtagccgggg ttggcgtaga cgttgatgtc gcggggctcg
     2341 gagtgcccga tgatcgtcat gtcgtaatcg ccaccgagga agatgcgctg gatccaggtg
     2401 ccccactcga cgacgctcag ctcgacgttt atgccgatgt cgcgcagcat ctgggctacc
     2461 acttcgccgc tgcgccgctc gatgacgtaa ggttcgggca gctcgaagtg gatggtgagg
     2521 ccgtcggcgt agccagcctc ggcgaggagt tccttggcgc gctgcgggtc gtacgggtac
     2581 gtgttcgaga ggtcgatgta gtacggctcg gaggggctca tatgggagcc gatgaccgtg
     2641 ccgagaccga acatggcgcc ttgcacgatg gcctgcttgt cgatcgccat ggtgatggcc
     2701 tggcgcacgc gtacatcgtc gaagggggga cgggtgttgt tcatcgccac cgtgatctcg
     2761 gtggtcatgg tgccctctgt gcccttcaga tcggggctac cgcccaacac caggtactgc
     2821 tcggacgtga cgcccgtcag gacgatgtcg ccggcctgca gcgcggcgaa acgcgtgttg
     2881 gcgtcgctga tgatcttgaa cacggccttg tcgaggtacg gcacgcccgc gacgtagtag
     2941 tcggggttgc gctcgagtac gacctcgctg ccttccgtgt aagacgcgaa cttgaagggg
     3001 ccggtgccga tgggctgcga gcgctgggtg tcggccttcg ccgcggggta gatgatcgag
     3061 tcggggcggg cgaggtcgta gaggaggccg cggctcggc
//
LOCUS       DAOQYE010000130         3093 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_33594_length_3093_cov_2.845951, whole genome shotgun sequence.
ACCESSION   DAOQYE010000130 DAOQYE010000000
VERSION     DAOQYE010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 3093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3093
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33594_length_3093_cov_2.845951"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..827
                     /locus_tag="PKN52_03155"
     CDS             <1..827
                     /locus_tag="PKN52_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98971.1"
                     /translation="ALVTPLERFQQFRSAYLLGSQNGPLVVAYHQSLELDLSRSASFD
                     IVSGAAGAADQALVQGVRDTVTEAVLQERYADIAVPAAGPAAVAVAYSADLAAGRQWV
                     VARTAAELDAAAPDLPADLRTLIRDDLAAGRLAVVPPGGAGAVGWWSLDQATGAVLGI
                     GDRGWGQAFSEYTEGVNIVLQLRTVLNQYASIGRCLGAMLSMPLQGKTQGLDSEFAEC
                     VFTTVCSGINTALAFGPDVNWTNIIILFTIDDLWGGMPETGFGGLCGGLYKKLIGG"
     gene            complement(848..1498)
                     /locus_tag="PKN52_03160"
     CDS             complement(848..1498)
                     /locus_tag="PKN52_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNQ98972.1"
                     /translation="MTRRAIRLMLVDDHALVRSGVRALLEAHDSPTTEFEVVGEASTA
                     EQALQLVGALAPDVVLLDLSLPGRSGVDALGDLAAAAPQARFVALSMHEDAEYVQRFL
                     AAGGSGYVPKTSLEAELVDAILAVDRGEYYVPARLLAVLTRELAHPDPRKAVQLTERE
                     TDVVRGIASGSTYREIAQTLGLSEKTVATYRERASEKLGARSRAELVRWAVERGLV"
     gene            complement(1507..2193)
                     /locus_tag="PKN52_03165"
     CDS             complement(1507..2193)
                     /locus_tag="PKN52_03165"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNQ98973.1"
                     /translation="MSPADAALLRATLSAQDQERARIATELHEDVGQALKAIVLGLDR
                     LGATDGDAARAERQRLRTAAVRSLEAVRRIALESRPPTLDEFGLGAAIRTLAREAEAR
                     GGPPVGVLVTAPGRDEVVGATGLRPERTGKASEAEVALFRVAKEALSNVVQHARATAA
                     SVVLSRTADRWSLVVEDDGVGFDPEALAPDKGLGLACMRARLVALGGDLDVESSPGGG
                     TSVYGRLNAH"
     gene            complement(2213..2800)
                     /locus_tag="PKN52_03170"
     CDS             complement(2213..2800)
                     /locus_tag="PKN52_03170"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNQ98974.1"
                     /translation="MSPTTRSERVRVLVPLDGSGFARQVIPYAIKVLRPDAHALTLLK
                     VAPVPEGYSSVAARPLTLEGWSARDGWTGGEPPVYYSQVYDSAVANLEEEVIADARRL
                     VAAGFDVTPEVRFGDPADEIVSLVDEEGYDLVVMATHGRSGLGRVLMGSVAGAVLKRV
                     HVPVMMVRPLPEGAGEELGALGDRVPVKATGEATT"
     gene            complement(2830..>3093)
                     /locus_tag="PKN52_03175"
     CDS             complement(2830..>3093)
                     /locus_tag="PKN52_03175"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNQ98975.1"
                     /translation="LREAGLKLLEREKANAAAVGVVAGTSLVEHHDPVEAIHDAEADH
                     DLVVMGTHGRRGFNRWMFGSVAEGALRRSTKPYLVVRSQEAGD"
BASE COUNT          432 a         1134 c         1078 g          449 t
ORIGIN      
        1 ccgcgctcgt cacgcccctc gagcgcttcc agcagttcag gagcgcctac ctgctcggga
       61 gccagaacgg tccgctcgtg gtcgcctacc accagagcct ggagcttgat ctgagccgca
      121 gtgcttcctt cgatatcgtg tcaggagcgg cgggcgccgc cgaccaggcg ctcgtccagg
      181 gcgtgaggga caccgtcact gaagccgtcc tccaggagcg ttacgccgat atcgccgttc
      241 cggcggccgg tccggcggcg gtcgccgtcg cctactcggc cgacctcgcc gccgggcgcc
      301 aatgggtggt cgcccgcacg gccgcagaac tggacgcggc cgcacccgac ctgccggccg
      361 acctgcgcac gctgatcagg gacgacctgg cagccggccg gctggccgtc gttccgcccg
      421 gcggagccgg cgccgtcggc tggtggagcc tcgaccaggc caccggagcc gtgctgggca
      481 tcggcgaccg cggctggggg caggcgttct cggagtacac ggagggcgtg aacatcgtcc
      541 tgcagctgcg cacggtgctc aaccagtacg cctccatcgg ccggtgcctc ggggccatgc
      601 tctccatgcc gctgcagggc aagacccaag ggctggacag cgagttcgcg gagtgcgtgt
      661 tcaccaccgt ctgctcgggc atcaacacgg cgctagcctt cggccccgac gtgaactgga
      721 cgaacatcat catcctgttc acgatcgacg acctctgggg cggcatgccg gagaccggtt
      781 tcggcggact ctgcggcgga ctgtacaaga agctgatcgg cggataggcc ggcggctccg
      841 cggaccgtca gacgagcccc cgctcgaccg cccacctcac gagctcggcg cgcgaccgcg
      901 cgccgagctt ctcgctggcg cgttcccggt acgtggcgac ggtcttctcg ctcaggccaa
      961 gggtctgcgc gatctcgcgg tacgtggagc cggacgcgat gccgcgcacc acgtccgtct
     1021 cgcgctcggt gagttgcacg gccttgcgcg gatcggggtg cgccagctcg cgcgtcagga
     1081 cggccaggag ccgggccggc acgtagtact cgccgcggtc gacggccagg atggcatcga
     1141 cgagttccgc ctccagcgac gtcttcggca cgtagccgga gccgccggca gcgaggaagc
     1201 gctggacgta ctcggcgtcc tcgtgcatgg atagggccac gaagcgcgcc tgcggcgccg
     1261 ccgccgcgag gtcgcccagg gcgtcgacgc cgctgcggcc gggcagcgat aggtcgagca
     1321 gcacgacgtc cggcgccagc gctcctacga gttggagcgc ctgctcggcc gtggaggcct
     1381 cgccgaccac ctcgaactcc gtggtcggcg agtcgtgagc ctccagtagc gcccttacgc
     1441 ccgacctgac gagggcgtgg tcatcgacga gcatgaggcg gatggcgcgt cgcgtcatac
     1501 cccaagctaa tgcgcgttca gcctgccgta cacggacgtt cctccccccg gcgagctctc
     1561 cacgtccagg tcgccgccca gcgcaacgag gcgcgcgcgc atgcaggcca gcccgagccc
     1621 tttatcgggt gccagagcct ccggatcgaa cccgacgccg tcgtcctcga ccacgaggga
     1681 ccacctgtcc gcggtgcgtg acaggacgac ggacgccgcc gtggcgcgcg cgtgctgcac
     1741 gacgttggag agcgcctcct tggcgacgcg gaagagggcg acctcggcct cgctggcctt
     1801 gccggtcctc tcggggcgca agccggtcgc gccaacgacc tcgtcacgcc ccggggcagt
     1861 gaccagtacg ccgaccggcg gaccgccccg cgcctccgcc tcgcgagcga gcgtccggat
     1921 ggcggccccg agcccgaact cgtcgagcgt cggcggtcgg ctctccaagg cgatgcgcct
     1981 gacggcctcc aggctccgca cggccgccgt gcgcaggcgc tgcctctcgg ccctggcggc
     2041 gtccccgtcg gtggcaccga ggcggtcgag cccgaggacg atcgccttga gcgcctgccc
     2101 cacgtcctcg tggagctcgg tggcgatgcg ggcccgttcc tgatcctgag cgctcagggt
     2161 cgctctcagg agcgcggcgt cggcaggcga catggtggcg gtctgcctcc ccctaggtcg
     2221 tggcttcgcc cgtcgccttc acaggcacgc ggtcgcccag cgccccgagc tcctccccgg
     2281 cgccctccgg caacggcctg accatcatga cgggcacgtg aacgcgcttc aggacggcgc
     2341 cggcgacgct tcccatgagg acgcgtccga ggccggagcg cccgtgcgta gccatcacca
     2401 ccaggtcgta gccttcctcg tcgacgagcg agacgatctc gtcggccggg tcgccgaagc
     2461 gcacctccgg cgtcacgtcg aagcccgcgg cgacgaggcg ccgcgcgtcg gcgatgacct
     2521 cctcctcgag gttggcgacg gcgctgtcgt agacctggct gtagtagacg ggcggctcac
     2581 ctcctgtcca cccgtcgcgg gcagaccagc cttccagcgt gagcggccgc gccgcgacgg
     2641 agctatagcc ctctgggacc ggcgcgacct tgagcagcgt gagggcgtgc gcatcagggc
     2701 gcaacacctt gatcgcgtag gggatcactt ggcgggcgaa cccggaaccg tcgagcggga
     2761 cgagaacccg cacgcgctcg ctcctcgttg tgggactcat acgtgactcc ttagggtcga
     2821 cgacccaact cagtcgccgg cttcctgcga cctgaccacc aggtacggct tcgtggaccg
     2881 gcgcagcgcg ccctcggcga ccgagccgaa catccatcgg ttgaagcccc tgcgaccgtg
     2941 cgtgcccatg acgacgaggt cgtggtcggc ctcggcgtcg tgaatggcct cgacggggtc
     3001 gtggtgctcg acgaggctgg tgccggcgac gacgccgacg gcggcggcgt tcgccttctc
     3061 gcgttcgagc agcttgagac cggcctcgcg cag
//
LOCUS       DAOQYE010000131         3092 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_33608_length_3092_cov_2.827461, whole genome shotgun sequence.
ACCESSION   DAOQYE010000131 DAOQYE010000000
VERSION     DAOQYE010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 3092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3092
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33608_length_3092_cov_2.827461"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1269)
                     /locus_tag="PKN52_03180"
     CDS             complement(<1..1269)
                     /locus_tag="PKN52_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HNQ98976.1"
                     /translation="MQVSDNTLTKLSFDRVRSALAERAGTFMGAELARALAPLPDEAA
                     AERALERVREVVEGGFLALGGIEDVRGLVQRVRDGNLLDGTDILSIAYTLDGAATLRR
                     AILVSERPALAELALEIGTFDGVLRLVREQLDPDGNVRDDATPKLYDIRRRLNPLRGR
                     IRDRLTRLLEQYSEFVQDPIITLRRERYVIPIKANHQARVSGLALDRSDSGATVFIEP
                     AAVVDLNNELALLEMEERDEVRRILLALGRRLADEPRLDASLEVVAKLDVVNASARLA
                     SEWRLARPAFDATGRVRLLGARHPLVTDCVPNDILLDEGTRLIVITGPNAGGKTVLIK
                     TVGLAALMAYSGLFVAADAARQPLLPYTRELLCDIGDEQSIEASLSTYAGHLRNLRAI
                     VDSADEHALVLIDELGSGTDPDEGAAISQAI"
     gene            1319..2440
                     /locus_tag="PKN52_03185"
     CDS             1319..2440
                     /locus_tag="PKN52_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98977.1"
                     /translation="MTEAREDKDCASVSRSDYVSRSDAGAQGDRAPRGDAGARGANAA
                     AELDLTAAPLFDWRHTLAALADWTAAPDRGRPTHDGAGWLMALRVGSHPGKLVVAELR
                     PGRAEGCLSVRLGHGARPGADAGITVSEVADAESQLRWRLALELDTRSLHEAAKRDPA
                     FEPVAAALAGFHPPLFSSAFQAACWTVVRQRTPPAFAAASFGRIAEFLGEEVVSSPAL
                     PTGGASERRPGDPTAGPLLVFPTPAALGEGARPALLAATNNLRKVDRLRGLAEAFLGV
                     DEAWLRTAAYDEAFAWLAMLPGLGPWSAEQVAWRGLGRYERTPWRDTGALSAVGAVYA
                     GGLTPALGSARELAERYGWQQGLWLRYLKAYPRVSGAKG"
     gene            complement(2451..>3092)
                     /gene="pta"
                     /locus_tag="PKN52_03190"
     CDS             complement(2451..>3092)
                     /gene="pta"
                     /locus_tag="PKN52_03190"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HNQ98978.1"
                     /translation="EVDGLVSGAVHTTASTIRPALQLIKTHKGARLVSSVFFMCLPDQ
                     VLVYGDCAVNPDPDAEELADIAVQSAASAAAFGIEPRVALLSYSTGTSGAGADVDKVR
                     EATRIARERRPDLPIDGPLQYDAAAVPSVASSKAPDSPVAGRATVLVFPDLNSGNATY
                     KAVQRSAGVVSVGPMLQGLRRPVNDLSRGALVDDIVYTIALTAVQATQVDAAD"
BASE COUNT          414 a         1045 c         1186 g          447 t
ORIGIN      
        1 gatggcctgc gagatggccg cgccctcgtc cggatcggtg ccggagccaa gctcgtcgat
       61 gagtacgagc gcgtgctcat cggcgctgtc gacgatggcg cgcaagttac gcaagtgacc
      121 ggcgtacgtg ctgaggctgg cctcgatgct ctgctcatcg ccaatgtcgc agaggagttc
      181 ccgcgtgtac gggagaaggg gctggcgggc ggcgtcggcc gccacgaaca ggcccgagta
      241 ggccatgagc gcggccagcc cgaccgtctt gatcagcacc gtcttgccgc ccgcgttcgg
      301 accggtgatg acgatcagcc gcgtgccctc gtcgagaagg atgtcgttgg gcacgcagtc
      361 tgtcacgagc gggtggcggg cgccgagcag gcgcacgcgc ccggtggcgt cgaaggccgg
      421 cctcgccagc cgccattcgg acgctaggcg ggccgacgcg ttgaccacgt cgagcttggc
      481 gacgacctcg aggctggcgt cgagccgcgg ctcgtcggcg aggcggcgac ccagcgcgag
      541 taggatgcgc cgcacctcgt cgcgctcctc catctccagc agcgcgagct cgttgttcag
      601 gtcgacgacg gcggcgggct cgatgaacac cgtggcgccg gagtcggagc ggtcgagggc
      661 gaggccggac acgcgcgcct ggtggttggc cttgatcggg atgacgtagc gctcgcgccg
      721 cagcgtgatg atgggatcct gcacgaactc cgagtactgc tcgagcagtc gcgtcagtcg
      781 atcgcggatc cggccgcgca gcgggttgag ccgacgcctg atgtcgtaga gcttcggcgt
      841 ggcgtcgtcg cgaacgttgc cgtcggggtc gagctgctcc ctcacgaggc gcagtacgcc
      901 gtcgaacgtg ccgatctcga gcgcgagctc ggcgagcgcg ggccgctcgg aaacgaggat
      961 ggcgcggcgc aacgtggcgg cgccgtcgag cgtgtaggcg atggacagga tatcggtacc
     1021 gtccagcagg ttgccgtcgc gcacgcgctg cacgaggccg cgcacgtcct cgatgccgcc
     1081 caaggcgagg aagccgccct ccaccacttc cctgacgcgc tccagcgccc gctcggcggc
     1141 cgcctcgtcc ggcagcggcg ccagcgcgcg ggccagctcg gctcccatga aggtgccggc
     1201 gcgttccgcg agcgccgagc gcacccggtc gaacgagagc ttggtcaggg tgttgtcgga
     1261 tacctgcacg gcgaacaagt atacgtgcca ggtcggttac gctttgcatc tatgaggcgt
     1321 gaccgaggcg cgcgaggaca aggattgcgc gagcgtgtcg cgtagcgact acgtgtcgcg
     1381 tagcgacgcc ggggcgcaag gcgaccgtgc accgcgcggc gacgccgggg cgcgcggtgc
     1441 caacgccgcg gcggaactgg acctgacggc cgcgcccctc ttcgactggc gccacacgct
     1501 cgcggcgttg gccgactgga cggcggcccc agaccgcggg cgccccaccc acgacggcgc
     1561 cggttggctc atggcgctgc gcgtcgggtc gcaccctggc aagctcgtcg tggccgagtt
     1621 gcgaccgggc cgggcggagg gttgtctctc cgtgcgcctt ggtcatggcg ctcgaccagg
     1681 tgcggacgcc ggcatcacgg tctcagaggt ggccgacgcg gagtcgcaac tccggtggcg
     1741 cctggcgctg gagcttgaca cccggtcctt gcacgaggcg gcgaagcgcg acccggcgtt
     1801 cgagccggtc gctgccgccc tggcggggtt ccacccgccc ctgttctcaa gcgctttcca
     1861 ggcggcgtgc tggaccgtgg tgaggcagcg cacgcctccg gcgttcgccg cggcgagctt
     1921 cggacgcatc gcggagttcc tgggggagga ggtcgtatcg tcgccggcgc tcccgacggg
     1981 cggggcgtcc gagcgacggc ccggcgatcc caccgccggc ccgctgctcg tctttcccac
     2041 cccggccgcg ctcggcgagg gtgcgcggcc ggcgctcctc gctgcgacga acaacctccg
     2101 caaggtcgac cgcctgcgcg gactggcgga ggccttcctc ggagtggacg aggcctggct
     2161 gcgcacggcc gcctacgacg aggctttcgc ctggctggcc atgctacccg gcctgggccc
     2221 atggtcggcc gagcaggtgg cgtggcgggg gctggggcgc tacgagcgca cgccctggcg
     2281 cgacaccggc gccctctcgg ccgtcggggc cgtctacgcg ggcgggctga cccccgcgct
     2341 cggcagtgcg cgagagctgg ccgaacgcta cggttggcag caagggctgt ggctgaggta
     2401 cctcaaggcg tacccgcgcg tgagcggcgc gaaggggtga gcggcgggcg tcagtcggcg
     2461 gcgtcgacct gcgtcgcctg cacggcggtg agagcgatcg tgtacacgat gtcgtcgacg
     2521 agcgccccgc gcgacaggtc gttgaccggc cgtctgagac cttggagcat ggggccgacg
     2581 gagacgaccc cggccgagcg ctgcacggcc ttgtaggtcg cgttcccgct gttgaggtcc
     2641 gggaagacga gcacggtcgc cctgccggcg acggggctgt ctggcgcctt gctgctcgcc
     2701 acgctcggca cggccgcggc gtcgtactgc agcgggccgt cgatggggag gtcggggcgc
     2761 cgctcccgcg cgatgcgggt cgcctcgcgc accttgtcga cgtcggcgcc cgcgcccgag
     2821 gtgcccgtcg aatagctgag gagcgcaacg cgcggctcga tgccgaaggc cgcggcggaa
     2881 gcggctgact gcacggcgat gtcggccaac tcctcggcgt cggggtcggg gttgacggcg
     2941 cagtcgccgt acacgaggac ctggtcgggt aggcacatga agaagacgga cgagacgagc
     3001 ctggcgccct tgtgggtctt gatgagctgc agcgcggggc ggatggtgct ggcggtcgtg
     3061 tgaacggcgc cggacacgag gccgtccacc tc
//
LOCUS       DAOQYE010000132         3086 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_33699_length_3086_cov_2.731772, whole genome shotgun sequence.
ACCESSION   DAOQYE010000132 DAOQYE010000000
VERSION     DAOQYE010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 3086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3086
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33699_length_3086_cov_2.731772"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1096)
                     /locus_tag="PKN52_03195"
     CDS             complement(<1..1096)
                     /locus_tag="PKN52_03195"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNQ98979.1"
                     /translation="MPDPAGNPFRIGEHVSGYHFADREEETARIRAAMLSASSLLVYG
                     PRRMGKSSTIANAAESARHSRPKPIVASVDMATATNLFDVSARLLGSLYRETRWLKLR
                     LEDLLGGLAPRVTVKLDERGGPPAITFGIDRRIAGEEERRQALEGVFDRFSRIREETG
                     RPVAVVLDEFQAVTSFAGESSEWHLRDVMQRHGDLAIICAGSQESLIHAMIGPKRAFY
                     KMFDMLPLAPIAPAVFAPWLEERLERGSGVAPGVGLELLRLAGPRTQDVVQVARQLYF
                     RGLAAWRKVDLADVQAALGDVVDGEAPLLRSIWGGLSAQQQDVLRVVALGVERLYSAE
                     VRDQYGLPGASSVHKAVQALIARGLLVTENE"
     gene            1238..2467
                     /locus_tag="PKN52_03200"
     CDS             1238..2467
                     /locus_tag="PKN52_03200"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNQ98980.1"
                     /translation="MQLDKLEVRKIGPIEEATLEFGGLTVFTGPQASGKSIALQLLKL
                     AIDNEHVLRTMVDKGLIWKGGTRQFFDLYLGSGMQSVWNRESSLRWQGEQLSLRTLTR
                     PSGPEGHGRAARTITEEQALYIPAQRMLCFRDGATRPFGDYRAGDPYVLREFSQHVHD
                     IVHGELSKGASQLFPKPQRLDEAIRDALATHVFGGWQLSVDTAGNQRELVLTHKKDPA
                     ADEQYPLRFLSWSSGQREFVPLLIGMYELMPAGAGSRRDPLRWAIIEEPESGLHPRAI
                     GATMALVYELIRREYRVVLSTHSTHVLDVVWGLRAMKEHGGGPDDALKLIELPSGRRT
                     RAAGVAVLEAVSRVYYFERGSVVADISALDPFSERQEEASWGGLTAFADNAAQVVAGV
                     VQRSQVAREGSSARRVR"
BASE COUNT          553 a         1020 c         1021 g          492 t
ORIGIN      
        1 tctcgttctc ggtcaccaac agcccgcgcg cgatgagcgc ctggacggcc ttgtgcacgg
       61 acgacgcccc cggcaggccg tactggtctc gcacctcggc ggagtacagg cgctccacgc
      121 cgagcgccac cacccgcagc acgtcctgct gctgcgccga gaggccgccc cagatgctgc
      181 gcagtagggg cgcctcgccg tcgaccacgt cccccagcgc cgcctgaacg tctgccaggt
      241 cgaccttgcg ccacgcggcg agcccgcgga agtagagctg ccgggcgacc tggacgacgt
      301 cctgcgtccg gggcccggcc aggcgcagca gctcgaggcc cacccctggc gccacgccgc
      361 tcccccgctc caggcgctcc tcgagccagg gcgcgaacac ggccggcgcg atgggcgcca
      421 gcggcagcat gtcgaacatc ttgtagaacg cccgtttcgg gccgatcatg gcgtggatca
      481 gcgactcctg cgacccggcg cagatgatgg cgaggtcgcc gtggcgctgc atgacgtcgc
      541 gcaggtgcca ttcggagctc tcgcccgcga acgacgtcac ggcctggaac tcgtcgagca
      601 ccacggcgac cggccgcccc gtctcctcgc ggatccggct gaagcggtcg aagaccccct
      661 ccagcgcctg ccgccgctcc tcctcgcccg ctatgcgccg gtcgatgccg aacgtgatgg
      721 ccggcgggcc gccgcgctcg tccagcttca cggtcacgcg cggcgccagc ccaccgagga
      781 ggtcctcgag gcgcagcttc agccaccgcg tctcccggta cagcgagccg agtaaccgcg
      841 ccgagacgtc gaacaggttg gtggccgtcg ccatgtcgac gctcgccacg atgggcttgg
      901 gcctggagtg ccgcgccgac tcggccgcgt tggcgatcgt cgagctcttc cccatgcgcc
      961 gcggtccgta gacgagcagc gacgacgcgg agagcatggc cgcccggatg cgggccgtct
     1021 cctcttccct gtcggcgaag tggtagccgg agacgtgctc gccgatgcgg aaggggttgc
     1081 ctgctgggtc cggcatgagc acctgctttc accgagcagc gattcaccga tcagtgaatc
     1141 acttggtggt gaaatggtag cgggtgtcgg gagtagaagc aatgggcatc ggccgtgggc
     1201 acggcgaggc acgacttgca cgcggctaga atgcaccatg cagctcgaca agctcgaagt
     1261 gcggaagatc gggccgatcg aggaagccac cctcgagttc gggggtctga ccgtgttcac
     1321 cggaccgcag gcgtccggca agagcatcgc gctgcagctc ctcaagctcg ccatcgacaa
     1381 cgagcacgtg ctgagaacga tggttgacaa gggcctgata tggaagggcg gcacgaggca
     1441 gttcttcgac ctctacctcg gctcggggat gcagtcggtc tggaaccgcg agtcatcgct
     1501 gcgctggcag ggtgagcaac tgagcctgcg caccctgacg cgccccagcg gacccgaagg
     1561 tcatggccgc gcagccagga ccatcaccga agaacaggcg ctctatatcc ccgcgcaaag
     1621 gatgctgtgc ttcagggacg gcgccacccg cccgttcggt gactatcggg cgggggatcc
     1681 ttacgtattg cgagagttca gccaacacgt ccacgacatc gttcatggcg aactgagcaa
     1741 aggcgcctct cagctctttc cgaagcctca gcggctggac gaagcgataa gggacgcgct
     1801 tgcgacacac gtcttcggcg ggtggcagct ttcggtcgac accgccggta atcaacggga
     1861 actggtactg acccacaaga aggatccggc ggcggacgag cagtacccgc tgaggttcct
     1921 ctcctggtcc tcaggtcagc gtgagttcgt gcctctcctg atagggatgt acgaactcat
     1981 gccagcagga gcgggcagcc gacgcgaccc actaaggtgg gcgatcatcg aggagccgga
     2041 gtcggggctt cacccgcggg ccatcggcgc cacgatggcc ctggtctacg aactgatccg
     2101 gcgcgagtac cgtgtcgtgc tgtcgaccca ctccacgcac gtgctggacg tcgtgtgggg
     2161 tttgagggcc atgaaggagc acggcggagg tccggacgac gcattgaagc tcatcgagct
     2221 gccttccggc cgtcgtaccc gagccgccgg cgtagccgtt ctcgaggctg tctcacgcgt
     2281 ctactacttc gagcgcgggt cggtggtagc ggacatatcc gccctcgatc ccttctccga
     2341 gcgacaggaa gaggcaagtt ggggcggcct caccgcgttc gccgacaacg ccgcccaggt
     2401 agtcgcaggc gtagtgcaga gaagccaagt tgcaagggaa ggatcaagtg cgcgacgagt
     2461 tcgttagcct tgggcagccg tacgcatcag ccgtgaaacc tgggctgcag gttctcgcca
     2521 ggcagcacag gaggctggta accatacggg actcccgtcg cctcaccgac agcatcgacc
     2581 tggacaccac gctctcgacc catccccagc acgcctctgc cgctcgctgg gactacggga
     2641 tagggttcca accttcagac gcctccccag aaggcgccgt ttgggttgaa gttcatcggg
     2701 ctcgaccctc ttccgtccga gagatggtag ccaaggcgcg ctggctcacg ggctggcttg
     2761 aatcggacgg tagcttgttg agggatccga cgcagcgcgg gcagagagag ttgggctccg
     2821 cattcttctg ggtcgcggaa ggatcagaca gcctcgcccg aaccggtagc gttcagaaga
     2881 agctggccag cgtcggcttg gccttcccca gccgtaagct cgatctgccg tagggatcga
     2941 gagcctcatc ggccactgcg tgatgcgcac aaagacttgc gtgatgcgca caaagattaa
     3001 agataggcgg cgaggtagct gcgtcttaag gagtcgagtc ttgcggacag tcaacacgac
     3061 cgaagcacga gagaacctgg tcgagc
//
LOCUS       DAOQYE010000133         3062 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_34070_length_3062_cov_2.643499, whole genome shotgun sequence.
ACCESSION   DAOQYE010000133 DAOQYE010000000
VERSION     DAOQYE010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34070_length_3062_cov_2.643499"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..943
                     /locus_tag="PKN52_03205"
     CDS             <1..943
                     /locus_tag="PKN52_03205"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HNQ98981.1"
                     /translation="VFELLNEPNDALDDATWNELVPQALAVIRETNPARWVVVGPTGF
                     NGIGSLPGLHWPADDHLVLTVHYYDPFYFTHQGADWVDPSPPVGVAWTGTRLTPSATW
                     ADWSWDTTRDYGNELSVTFEAAWAGYYLQPLATVFGYDQLAFTTSRAVALLVACGPGD
                     VAAPVSVAVDTLPGVETVIDLDACGGADGVVRVVLQDRSGVAQAPFVLQGIELRAVGK
                     HLPLLVTETQAIGAAFDYLKAWAGAHGAPPVLIGEFGAFQEADMASRVRWTRAVREAA
                     EARGLNWAYWEFADGFGAYDPVAEAWRTGLLDALVGD"
     gene            974..1582
                     /locus_tag="PKN52_03210"
     CDS             974..1582
                     /locus_tag="PKN52_03210"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HNQ98982.1"
                     /translation="MRKVIVSNFATMDGYYEAPGKTIEPFFEHLHPDYRGDQAFDHHN
                     VERLSAADTLLLSGKTSFLGNQAYWTGVEADPAATPIRREFSRLIREVEKVVVSDRLG
                     AGDLGAWSGSTTVVSVASAAAHVRALKQGAGRDVLVLMGRLLWNHLLAEGLVDELHIT
                     YFPLVAGGGVPLFERRPQTTFKLLGARTYPGSGIVTNRYAVG"
     gene            complement(1676..2626)
                     /locus_tag="PKN52_03215"
     CDS             complement(1676..2626)
                     /locus_tag="PKN52_03215"
                     /inference="COORDINATES: protein motif:HMM:TIGR03718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HNQ98983.1"
                     /translation="MTEPLWAWFAFVGFVLAMLAIDLGVFQRKAHVVKPQEAALWTGV
                     WVSLSLLLGLVFYFWLGSEKAIQYVTGYVLEWSLSVDNIFVFVLVFTYFKTPFKYQQR
                     VLLWGILGALAMRAIMIVVGAALIQAFSWVLYIFGAFLLFTGARMLFQKGEGETDLEK
                     NPGLRLVRRLIPVSSEYDGQKFFTIKNGVRMATPLLLVLVVIEFTDVLFAVDSIPAIF
                     AVTTDGFIVFTASIMAVLGLRSMYFLLAHVVHRFVYLKTGLAIILVYIGAKMLLLDVL
                     HIPSLVSLAVVLFILAVSVVASLVWGKPQVPAHHESVPPS"
     gene            2801..>3062
                     /locus_tag="PKN52_03220"
     CDS             2801..>3062
                     /locus_tag="PKN52_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98984.1"
                     /translation="MAVQAEKRLTLEELRADVIGEGACFATPFGARRLTYADHIASGR
                     PLASVEESLRRAVLPLYANTHTEDSATGAHTTELTELVRDYLK"
BASE COUNT          537 a          997 c         1042 g          486 t
ORIGIN      
        1 cgtcttcgag cttctcaacg agcccaacga tgccttggac gacgccacct ggaacgagct
       61 cgttcctcag gcgctggccg taattcgcga gaccaaccct gccaggtggg tcgtggtcgg
      121 gccgacaggc ttcaacggca tcggctcgct gcccggcctg cactggccgg ccgatgacca
      181 cctggttctc accgtgcact actacgaccc gttctacttc acgcaccagg gggccgactg
      241 ggtggatccg tcgccgcctg tcggcgtcgc atggacggga acgcgactca cgccgagcgc
      301 gacgtgggcc gactggtcgt gggacacgac ccgggactac gggaacgagt tgagcgttac
      361 gttcgaggcg gcctgggcag gttactacct gcagccgctc gcgaccgtct tcggctacga
      421 ccagctggcg ttcacgacct cgcgcgccgt cgccctgctg gtcgcctgtg gtcccggcga
      481 cgtggcggca cccgtgtccg tggccgtaga caccctgccg ggggtcgaga cggtcataga
      541 tctggacgcc tgcggaggcg cggacggggt cgtccgtgtc gtccttcagg accgctcagg
      601 cgtcgctcag gcacccttcg tcctccaagg catcgagctg cgggcggtgg gcaagcacct
      661 tccgctcctc gtcacggaga cgcaagcgat aggcgccgcg ttcgactacc tcaaggcgtg
      721 ggcgggggcg cacggcgcgc cgccggtcct catcggcgag ttcggggctt tccaggaggc
      781 ggacatggcg tcgcgtgtca ggtggacccg cgccgtgcgc gaagccgccg aagcccgggg
      841 tctcaactgg gcctactggg agttcgcgga cggcttcggg gcgtacgacc cggtggccga
      901 agcgtggcgt acgggactgc tcgacgcgct cgtcggggat tgaggtcgcc caggagcgaa
      961 acggaggtcg gacatgcgca aggtcatagt cagcaacttc gccaccatgg acggctacta
     1021 cgaggcgccc ggcaagacca tcgagccgtt cttcgagcat ctccatccgg actatcgagg
     1081 tgaccaggcg ttcgaccatc acaacgtgga gaggctgagc gcggccgaca cgctgttgct
     1141 gagcggcaag acctcgttcc tcgggaacca ggcctactgg accggcgtcg aggcagatcc
     1201 ggccgccact cccatccggc gcgagttctc caggctcatc cgggaggtgg agaaggtcgt
     1261 ggtctcggac agactgggtg ctggcgacct cggcgcttgg agcggcagca ctaccgtcgt
     1321 gtccgttgcg tccgccgccg cccacgttcg tgcgctcaag caaggcgccg ggcgcgacgt
     1381 cctcgtcctg atggggcggc tgttgtggaa ccacctgctg gccgaaggcc tggtggacga
     1441 gttgcacatc acgtacttcc cgttggtggc cgggggcggc gtgccgttgt tcgagcgccg
     1501 cccccagacg accttcaagc tccttggcgc gcgcacctat cccggctcgg ggatcgtcac
     1561 caaccggtac gcggtcggat gaggacgccg ctgggcggta acccgtcggc cccgccggct
     1621 gcgttcgtca gaccccgatg agaatcgggc ccgtaggaac cgtttgggct cgcgctcagc
     1681 ttggcggaac cgactcgtgg tgcgcgggca cctggggctt gccccatacc aaggacgcca
     1741 ccacgcttac cgccaggatg aagaggacca ccgcaagcga cacgaggctc gggatgtgca
     1801 gcacgtcgag gagcagcatc ttcgcgccga tgtagacgag gatgatggcg agccccgtct
     1861 tgaggtagac gaagcggtgg acgacgtgcg ccagcaggaa gtacatggag cgcaggccga
     1921 gcacggccat gatgctggcc gtgaacacga tgaagccgtc cgtggtgacc gcgaagatgg
     1981 ccgggatcga gtcgacggcg aagagcacgt cggtgaactc gatcacgacc agcacgagca
     2041 ggaggggcgt ggccatgcgc acgccgttct tgatggtgaa gaacttctgc ccgtcgtact
     2101 cggaactgac cggtatcagt cgcctcacga ggcgcaagcc cgggttcttc tccaggtccg
     2161 tctcgccctc acccttctgg aagagcatgc gggcgcccgt gaagaggagg aaggccccga
     2221 agatgtagag cacccaactg aacgcctgga tgagcgcggc gccgaccacg atcatgatgg
     2281 cgcgcatcgc tagggcgccg aggatgcccc ataggagcac gcgctgctgg tacttgaaag
     2341 gcgtcttgaa gtaggtgaag acgagcacga agacgaagat gttgtcgacg gacagcgacc
     2401 attcgagcac gtagcccgtt acgtactgga tggccttctc ggacccgagc cagaagtaga
     2461 acaccagccc gaggagcagt gacaggctca cccagacgcc cgtccagagg gcggcctcct
     2521 gtggcttcac cacgtgcgcc ttgcgctgga agaccccgag atcgatggca agcatcgcga
     2581 ggacgaagcc gacgaaggcg aaccaggccc atagcggttc ggtcattaag acctcccgtt
     2641 agggtctcgc ctacgcgcgc gccaccgggc ggcctttgac cgccgtgctg acgatggcgt
     2701 gctcccgcgg ggcgcgggcg gctactcccc ctcaacggtc agcgagttta acctggcgac
     2761 cactaggatg taacctgagt cgataccggc tggaggtgag gtggcagtgc aggcagagaa
     2821 gcgactcacc ctagaggagc tgcgggccga cgtcatcggg gaaggcgctt gtttcgccac
     2881 gcctttcggt gcccggcgct tgacctacgc cgaccacatc gcctccggcc gcccgctggc
     2941 ctccgtcgag gagtccctca ggcgcgcggt gctcccccta tacgccaaca cccacaccga
     3001 ggacagcgcg accggcgcgc atacgaccga gttgaccgaa ctggtgcgcg actacttgaa
     3061 gc
//
LOCUS       DAOQYE010000134         3058 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_34144_length_3058_cov_2.818182, whole genome shotgun sequence.
ACCESSION   DAOQYE010000134 DAOQYE010000000
VERSION     DAOQYE010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 3058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3058
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34144_length_3058_cov_2.818182"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..758
                     /locus_tag="PKN52_03225"
     CDS             <1..758
                     /locus_tag="PKN52_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98985.1"
                     /translation="FASQSLVLTQLQAMRLMQLMAGVAGFIRQAAAAAPRRRPNRSLP
                     LPAAQSPVWRAFAWRALTHLWRAPWWRQLTTLVLVALAGGLAALAGAGGGAAAPGVGV
                     APATAGGLAGLFGGPLAVFAAAYLVARAGAALLGPEFAIGVAPVDPYARTLGRVFPAA
                     AAILFALLVASGFLGDAAGGLFSGVALVLTVLLVVEKYGSWSGSGAARWEAQLVAALL
                     AALPAFVLSAFGVPDWTFGTQIGLLLVVSLVGV"
     gene            862..1791
                     /locus_tag="PKN52_03230"
     CDS             862..1791
                     /locus_tag="PKN52_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98986.1"
                     /translation="MRIRTFVLVALATCLTLAFAQQFNGTFVDQANGISIAFQQGQNG
                     ALSGAVITGNGQFPLQGQADGQYAYGAVQSQQGVLGFQAQVSPDGSMLQIQFYEMGPN
                     GQPVGQGQVMVLQRQGAGQTGFPPPPPGAVPVNPGAGTQPGMPTQPGMPTQPGMPTQP
                     GMPTQPGFGTPTQPGFGTQPGMPTQPGMPTQPGMPTQPGFGTQPGMPTQPGAPGQPGL
                     GGAQTGDMTGTYVGNNGAFVLSLQSAQGGYVGYIQDQGQRYQFQAHYDDDGSVHGQGN
                     QFEFWVYEDGGYTMLDIGDTSYMLQKTSNQPTP"
     gene            1899..2648
                     /locus_tag="PKN52_03235"
     CDS             1899..2648
                     /locus_tag="PKN52_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98987.1"
                     /translation="MLVSISRSVLVLLCLATVGVGAAQYYPYGYPYWFGYDPTMGGMY
                     GGTPYDPSMGAMYGNQYDPTMGGMYNGALDPMAQMDLIGRQIQARLDEYNKYFIDLYR
                     KTTGDMTSPDSYALQMGQAIHCQQYPLDCQLAYQGSKAAQASQQASFDAWMAGVKDRD
                     AANDAAMAAWWQQQQSQEAGVADFNRTVIREEDQYTNPDTGVTYDLPYAPSQTTYYQT
                     PTGLPLVFDPAFNVWYQINPDGSRTPYYGAP"
     gene            complement(2633..>3058)
                     /locus_tag="PKN52_03240"
     CDS             complement(2633..>3058)
                     /locus_tag="PKN52_03240"
                     /inference="COORDINATES: protein motif:HMM:NF015649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial transcriptional activator
                     domain-containing protein"
                     /protein_id="HNQ98988.1"
                     /translation="ELEAALARGDVASVSDLYQGPLAPGVDLDPVAQERLHLQERVVD
                     ALLAGAEASAARADGEVDGAGLLEDAVRYLKRLLELEPLHEEAVQALLGHLMRLGRRR
                     DAQRTLERFIQTLEQETGLAPQPATLAHLVKPGVNYGAP"
BASE COUNT          463 a         1089 c         1024 g          482 t
ORIGIN      
        1 gcttcgcttc gcagagccta gtgctcaccc agttgcaagc catgcgcctc atgcaactga
       61 tggcgggggt ggccgggttc atccgccaag ccgccgccgc cgctccccgc cgtcggccga
      121 acaggtcgct cccgctgccg gccgcccagt cgccggtgtg gcgggcgttc gcgtggcggg
      181 cgctgactca cctctggcgc gcgccatggt ggcggcagct cacgacgctc gtgctggtgg
      241 ccctagccgg cggtctcgcg gcgctggccg gagccggggg cggcgccgcc gcgccgggtg
      301 tcggcgtcgc gccggctacc gccggcgggc tggcgggcct gttcggaggg ccgctggccg
      361 tgttcgcggc cgcgtacctc gtggcgcgcg ccggggccgc gctcctcgga ccggagttcg
      421 ccatcggcgt cgcgcccgtc gatccgtacg ctcgcacgct cggcagggtc tttcctgcgg
      481 cggccgcgat cttgttcgcg ctgctcgtag cgagcggctt cctgggcgac gccgccggcg
      541 gcctgttctc cggtgtcgcg ctggtcctca ccgtcctcct ggtcgtagag aagtacggct
      601 cgtggagcgg cagtggcgct gcgcgctggg aggcccagct cgtggccgcc ctcctcgcag
      661 ccttaccggc tttcgtactg agcgctttcg gtgtgccgga ctggacgttc ggcacccaga
      721 tcggcctgtt gctcgtcgtc tccctcgtcg gggtatgagc ttaggggcgt ccgcccagga
      781 tgccgcgggt cggccgcgcg gcctattaag gctggtttaa gcgaccccct cctaacatcg
      841 gtgcacgtta aggaggagac cgtgcgcata cgcaccttcg tcttggtggc cttggctacg
      901 tgcctgaccc tggccttcgc ccagcagttc aacggcacgt tcgtcgacca ggccaacggc
      961 atcagcatcg ccttccagca gggccagaac ggcgctctca gcggcgccgt catcaccggc
     1021 aacggtcagt tccctctgca gggccaggct gatgggcagt acgcgtacgg cgccgtccag
     1081 tcgcagcagg gcgtgctcgg gttccaggcg caggtcagtc cggacggctc gatgctgcag
     1141 atccagttct acgagatggg accgaacggc cagccggtcg gccaaggaca ggtgatggtc
     1201 ctgcagcgcc aaggtgccgg ccagaccggt ttcccgccgc cacctcccgg cgccgtgccc
     1261 gtgaacccgg gcgccgggac ccaacccggc atgccgaccc agcctggcat gccgacgcag
     1321 cccgggatgc cgacgcaacc tgggatgccg acgcaacccg gtttcggcac accgacgcag
     1381 ccggggttcg gcacgcagcc tggtatgcct acgcagcctg gcatgccgac ccagccgggt
     1441 atgcctactc agcccggttt cggcacccag cccggcatgc cgacccaacc tggcgcgcca
     1501 gggcaacccg gcctcggcgg ggcgcagact ggcgacatga ccggcacgta cgtcggcaac
     1561 aacggcgcct tcgtcctctc tctgcagagc gcgcaaggcg gctacgtcgg ctacatccag
     1621 gaccaggggc agcgttacca gttccaggcg cactacgacg acgacggttc ggtccacggt
     1681 cagggcaacc agttcgagtt ctgggtctat gaggacggcg gctacacgat gctcgacatc
     1741 ggcgacacct cctacatgct tcagaagacc tccaaccagc ccacgccatg aacgtgtccg
     1801 cactcgatac cttcgcgctc gccgctctag acggtcggcg cggggaagct gccaggcgag
     1861 gccgcggcgc tgcccttggg ccgaggaagg aggctcggat gctcgtctcc atatcgcgga
     1921 gtgtgctggt gctgctctgc ttggcaacgg tcggggtcgg cgccgcccag tactacccgt
     1981 atggctatcc gtactggttc gggtacgacc ccaccatggg cgggatgtac ggcggaacgc
     2041 cctacgaccc ctccatgggg gccatgtacg gcaaccagta cgaccccacc atgggcggca
     2101 tgtacaacgg ggcgctcgat cccatggcgc agatggacct gatcggccgc cagatccagg
     2161 cgcggctcga cgagtacaac aagtacttca tagacctcta ccggaagacc accggcgaca
     2221 tgacctcacc ggactcctac gctctccaga tgggccaggc gatccactgc cagcagtacc
     2281 cgctcgactg ccagctcgcc tatcagggtt cgaaggcggc ccaagccagc cagcaggcca
     2341 gcttcgacgc ttggatggcg ggggtgaagg accgggacgc cgcgaacgat gcggccatgg
     2401 ccgcgtggtg gcagcaacag cagtcgcagg aggccggggt cgccgacttc aaccgcacgg
     2461 tcatccgcga agaggatcag tacacgaacc cggacaccgg cgtcacctac gacctgccgt
     2521 acgcaccgag ccagaccacc tattaccaga cgccgacggg gctgccgctc gtcttcgacc
     2581 cagccttcaa cgtctggtac cagatcaacc ctgacgggag caggacgccc tactacggcg
     2641 cgccgtagtt gacgcctggt ttgaccagat gggcgagcgt cgcgggctgg ggggccagcc
     2701 ccgtctcttg ctccaacgtc tggatgaagc gctccagggt ccgctgcgcg tcgcggcgac
     2761 gcccaagccg catgaggtgg ccgagaagcg cctgcacggc ctcctcatgc agcggctcga
     2821 gttcgagtag ccgcttcagg tagcggacgg cgtcctccag gaggccagcg ccgtccacct
     2881 cgccatcggc cctcgccgcg ctggcttcgg cgccggctaa cagggcgtcc acgacgcgct
     2941 cctgcaagtg gaggcgctcc tgggcaacgg ggtcgaggtc gactccgggc gcgagcggcc
     3001 cttggtacag gtcgctgacc gaggcgacgt cgccccttgc caacgccgcc tcgagttc
//
LOCUS       DAOQYE010000135         3054 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_34209_length_3054_cov_2.938980, whole genome shotgun sequence.
ACCESSION   DAOQYE010000135 DAOQYE010000000
VERSION     DAOQYE010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 3054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3054
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34209_length_3054_cov_2.938980"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="PKN52_03245"
     CDS             <1..186
                     /locus_tag="PKN52_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S oxidoreductase"
                     /protein_id="HNQ98989.1"
                     /translation="TETMLDDLTRDDLEVELGMRVAVGGTNLAELVDTILTGHVHDHL
                     PSFGFSTHAIKEAVKQH"
     gene            188..1072
                     /locus_tag="PKN52_03250"
     CDS             188..1072
                     /locus_tag="PKN52_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HNQ98990.1"
                     /translation="MTSGVRTGDKGRLTLATLASFVKFEHTLFALPFAYGGMLLAADG
                     WPGWRVFLLVTLAMVGARSAAMAANRVIDARIDALNPRTAAREIPTGALTVHDGWLVA
                     LVSLAVLAAAAALLNRLTLALLPVAVAFLIAYPYTKRFTWACHLWLGLTIGAAAAGGY
                     IAVSGAFAPSAWLLWLGVGSWVAGFDVVYALLDLGFDLEHGVRSIPARFGVRGARAWA
                     AALHVVTLLALAGVALVASLGWPYWLALAVTAGVLAWQHLSLRHRAVGEVLAAFNANL
                     VIGLLMLGGIVFGLVARG"
     gene            1197..1272
                     /locus_tag="PKN52_03255"
     tRNA            1197..1272
                     /locus_tag="PKN52_03255"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1230..1232,aa:Asp,seq:gtc)
     gene            1386..2045
                     /locus_tag="PKN52_03260"
     CDS             1386..2045
                     /locus_tag="PKN52_03260"
                     /inference="COORDINATES: protein motif:HMM:NF014269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HNQ98991.1"
                     /translation="MGKLPVFPLDVVVFPGMTVPLHAHEERYKRLVREVLAQEDEPKR
                     FIIVYSDAAPAITDVAPRMARFGTVVHVLTAEENVDGTFDLLVHGQERTRVEVDAVVE
                     VTERGGASRPLSYAEERLAPLYRGDPNEEAIAAWDALDTFRTYAGTRFDGDAGEEIDK
                     HVPDDPFYQASFVCANMLVPTAAKQTLLEAESLRERFDLARGMMLERMQRRGRAKRGR
                     A"
     gene            2042..3043
                     /gene="truD"
                     /locus_tag="PKN52_03265"
     CDS             2042..3043
                     /gene="truD"
                     /locus_tag="PKN52_03265"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HNQ98992.1"
                     /translation="MSEERSASEPVQGARGAVGGEERARFAFAWDDLPLVTSDLPGSG
                     GSIRAAPEDFRVTELPAYLPEGSGSHRYLLIEKRDLTTRDLVLALRRAGVEQNAIGVA
                     GLKDKAAVTVQWLSLPKRYTEAVSELLALPGVRLLEESYHRNKLGIGHLRGNRFEVTV
                     QGVDAGASERAGAVLASLAELGSPNWFGPQRFGRFGGNAYDGLRVVRGERVPGGHYLQ
                     RFFVSALQSLLFNAMLSERIGRGWYTTVVAGDWARKHDTGGTFLVADEAVEAPRARRL
                     EISATLPLHGRKVKPSEATAGEVEYLRRVTDTSQLQCSIIPGFPLVVTLLVGVRWAL"
BASE COUNT          414 a         1095 c         1053 g          492 t
ORIGIN      
        1 acggagacga tgctggacga cctcacccgc gacgacctcg aggtcgagct cggcatgcgg
       61 gtcgcggtcg gcggcacgaa cctggccgag ctcgtcgaca ccatcctcac gggccatgta
      121 cacgaccacc tgccgagctt cgggttctcg acccatgcca tcaaggaagc tgtcaagcag
      181 cactgacgtg accagcgggg tgcggaccgg cgacaaggga cggctgacgc tcgctacgct
      241 cgcgtcgttc gtcaagttcg agcacaccct cttcgcccta ccgttcgcgt acggcggcat
      301 gctcctagcg gccgacggct ggccgggctg gcgggtgttc ctgctcgtga ccctcgcgat
      361 ggtgggggcg aggagcgccg ccatggccgc caaccgggtc atcgacgccc gcatcgacgc
      421 cctcaacccg cgcacggccg ctcgtgagat ccctactggc gcgctgaccg tgcacgacgg
      481 gtggctcgtg gcgctcgtca gcctcgccgt gctggcagcc gcggccgccc tcctcaaccg
      541 cctgacgctc gccctgctgc ccgtcgccgt ggcgttcctg atcgcctacc cgtacaccaa
      601 gcgcttcacg tgggcctgcc acctgtggct cggactgacc atcggagcgg ccgcggccgg
      661 cggctacatc gccgtgagcg gcgcgttcgc gccgtcagcc tggctcctgt ggctcggtgt
      721 cggctcgtgg gtggcgggct tcgacgtggt gtacgccctg ctcgacctgg ggttcgacct
      781 ggagcacggc gtcaggtcca tccccgcgcg cttcggggtt cgcggcgctc gcgcatgggc
      841 ggccgccctg cacgtcgtca ctctcctcgc cctggcaggg gtggcgctcg tcgcctcgct
      901 cggctggcct tactggctcg cccttgccgt cacggcgggc gtgctggcct ggcagcacct
      961 gtccctgcgg caccgagcgg tgggcgaggt gctggccgcc ttcaacgcga acctcgtgat
     1021 cggccttctg atgctcggcg gcatcgtgtt cgggctggtg gcgcggggct gacgggcggt
     1081 gcggtggagg tggcggcagc cgcggcgcga cggcgacctt cgccgtgtcg acatgctcga
     1141 tcactaggga ccggccttga ccaggcccct accctcacgt acactcgcct tcgcgtggag
     1201 ccgtagtgta gcggttagca tatctgcctg tcacgcagaa ggtcgcgggt tcaaatcccg
     1261 tcggctccgc caagcgcagg gcgcccggca cgtcgctaag tgccgggcgc ccttcattgc
     1321 cgcatcgtga aggcgccgca cctcacgaat cacgtgtcgc cgccggcacc tggtaccgtt
     1381 tagacatggg caagttgccc gtcttcccct tggacgtcgt tgtcttccca ggcatgaccg
     1441 tcccactgca cgcccacgag gagcgctaca agcgcttggt gcgtgaggtg cttgcgcagg
     1501 aggacgaacc gaaacgtttc atcatcgtct actcggacgc cgccccggcc atcacggacg
     1561 tcgcgccgcg catggcccgc ttcggcacgg tcgtgcacgt cctgacggcg gaggagaacg
     1621 tcgacggcac cttcgacctg ctggtgcacg gtcaggagcg cacgcgcgtc gaggtcgacg
     1681 cggtcgtgga ggtcaccgag cgcggcggcg cgagccggcc cctgagctac gccgaggagc
     1741 gcctcgcccc cctgtaccgc ggcgacccga acgaggaggc catcgccgcc tgggacgccc
     1801 tcgacacgtt caggacctac gccggcacgc gcttcgatgg cgacgccggc gaggagatag
     1861 acaagcacgt gccggacgac cctttctacc aggcatcgtt cgtgtgcgcg aacatgctcg
     1921 tcccgaccgc cgccaagcag acgctcctgg aagcggagtc cttgcgcgag cgtttcgacc
     1981 tcgcgcgggg catgatgctc gagcgcatgc agcgtagggg gcgcgccaag cgcggacgcg
     2041 cgtgagcgag gagcgctcgg ccagcgagcc ggtgcagggc gcgcgcggcg cggtaggcgg
     2101 cgaagagcgc gcgcggttcg cgttcgcttg ggacgacctg cccctcgtca cctccgacct
     2161 gcccggcagc ggcggttcca tacgcgccgc gccggaggac ttccgggtca cggagttgcc
     2221 cgcctacctg ccggaaggct ccgggtcgca ccgttacctg ctcatcgaga agcgcgacct
     2281 gaccacgcgc gacctcgtgc tggcgctcag gcgcgccggc gtcgagcaga acgccatcgg
     2341 ggtggcgggc ctcaaggaca aggcggccgt cacggtgcag tggttgtcgc tccccaagcg
     2401 ctacaccgag gccgttagcg agttgctcgc gctgcccggc gtgcgactgc tcgaggagag
     2461 ctaccaccgc aacaagctcg ggatcggtca cctgcgtggc aaccgtttcg aggtgaccgt
     2521 acagggggtc gacgctggcg cctcggagcg tgccggggcc gtcctcgcca gcctagcgga
     2581 gctcgggtcc cccaactggt tcggcccgca acgcttcggc cgtttcggcg gcaacgccta
     2641 cgacggtctc agggtggtgc gcggcgagcg cgttccgggc ggccactacc tccagcgctt
     2701 cttcgtctcc gccctgcagt cgctcctctt caacgccatg ctctcggagc gcatcgggcg
     2761 cggctggtac accaccgtcg tcgccggcga ctgggcccgc aagcacgaca ccggcggtac
     2821 cttcctcgtc gcggacgagg cggtcgaggc cccgcgcgcg cggcgtctgg agatcagcgc
     2881 aacgctgccc ctccacgggc gcaaggtgaa gccgtcggaa gcgacggccg gcgaggtcga
     2941 gtatctgcgt cgggtcacgg acacctctca gttgcagtgc tccatcatcc ccggcttccc
     3001 gctggtcgtc accctcctgg tcggggtgcg ctgggcgctg tgacgtgccc gcgt
//
LOCUS       DAOQYE010000136         3046 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_34346_length_3046_cov_3.021063, whole genome shotgun sequence.
ACCESSION   DAOQYE010000136 DAOQYE010000000
VERSION     DAOQYE010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34346_length_3046_cov_3.021063"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..991)
                     /locus_tag="PKN52_03270"
     CDS             complement(<1..991)
                     /locus_tag="PKN52_03270"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNQ98993.1"
                     /translation="MKPPDTASSGLRVERRGDVVLLVIDQPARPVNLLDTALLGALSQ
                     AVEELVEDSGLVGAVLCSSRPGSFLAGADVSSFLDYTSPAEVEAAIGQGNALLGRIEG
                     CGKPLVVAVDGVCLGGGTELALAGRYLIASTSPKTRFGLPEVQLGLLPGLGGTVRLPE
                     RVGLTLALDLILTGRNLYARQAHRAGLAHATVHPEGLLEAAVKAARLLADGGLQVPKR
                     KPKLAQRLLKETPARAIAFDRAAKSVRERTHGNFPAPRRILEVLDAWARGGREAGLDA
                     SAKAFAELLFTPEARALIHLFFAQTAAKKNPFRGEALPVETVAVLGAGLMGSGI"
     gene            complement(988..1224)
                     /locus_tag="PKN52_03275"
     CDS             complement(988..1224)
                     /locus_tag="PKN52_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ98994.1"
                     /translation="MDGMRAEKAHAASLSGGSEARLSLVVIGDHVWDATLRSGPGLLS
                     GGDASGGDVSDEGADRYLLIGHALSIGRLPGGVA"
     gene            1248..2330
                     /locus_tag="PKN52_03280"
     CDS             1248..2330
                     /locus_tag="PKN52_03280"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HNQ98995.1"
                     /translation="MKARVAKLFENPFLRRVNWHLRRLSDQMEAGFFRSLLIGLVVIL
                     LFISLIVWAFETDHTLAVFGASFYWAATTVLGQGDASFASGPVGWVMGWLLGLFGVAI
                     VATITGALVGFMIDFLLKEGQGMGASGYRGHIVVCGWNATARNLIEELKEDEYGGRVV
                     LVYDSEKSPAGDGVYFVRGSDTSEADLKRAGIEHARSAIVCPSDGTDEADMHSILVVL
                     AIESVAPDVRTVVEVNNPKHTQHFRRAHVDEFLVTSVLSSHLLARSAMYPGLSELVTD
                     IVSGGEGSELYRVELPDDCVDKPIDEVSAWLRRTHAATLLAVAREGVTHTNPAADFKL
                     AAGDDLVVLAESLGRLRPYERAAAPA"
     gene            complement(2358..>3046)
                     /locus_tag="PKN52_03285"
     CDS             complement(2358..>3046)
                     /locus_tag="PKN52_03285"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HNQ98996.1"
                     /translation="HQGAAAHRDLLAEEIVPVSVPPKGQVVAADNGVRDDTSLERLAK
                     LPPAFVKPFGTVTAGNASQLTDGAAAVLVMGEQRAAAEGRTGLVRVRDFLFVARDPKD
                     ELLLGPAYAIPRLLARNGLAWGDIDVVELHEAFSGQVLAVLRALESDAFGREKLGLGG
                     AFAPEVGMDKVNPWGGSVSLGHPFGATGARLVATAAHRLNVGGGQFALVAACAAGGQG
                     HAMLLERVGP"
BASE COUNT          447 a         1089 c         1071 g          439 t
ORIGIN      
        1 cgatgcccga gcccatgagg cccgcgccga gcaccgccac cgtctcgaca ggcagcgcct
       61 cgcccctgaa ggggttcttc ttggccgccg tctgggcgaa gaacaggtgg atgagggcgc
      121 gcgcctccgg cgtgaagagg agctccgcga aagccttggc gctggcgtcc agcccggcct
      181 cgcgaccgcc acgcgcccag gcgtcgagaa cctcgaggat ccgcctcggt gcggggaagt
      241 tgccatgagt gcgctcccgg acggacttcg ccgccctgtc gaaggcgatg gcgcgagccg
      301 gcgtctcctt cagcagccgt tgcgcgagct tcggcttgcg cttaggcacc tgcaggccgc
      361 cgtcggccag tagcctggcc gccttcacgg ccgcctcgag caagccttcc ggatgcaccg
      421 tcgcgtgcgc gaggccggcc ctgtgtgcct gcctggcgta caggttgcgc ccggtgagga
      481 tgaggtcgag cgccagcgtg aggccgacgc gctccggcag gcgcactgtg ccgcccagcc
      541 ccggcaggag gccgagctgc acctccggca ggccgaagcg cgtcttgggg ctcgtgctgg
      601 cgatgaggta gcggccggcg agcgccagct ccgtgccacc gcccaggcac acaccgtcga
      661 ccgcgacgac gagcggcttg ccgcaaccct cgatgcggcc gagcagggcg ttgccttggc
      721 cgatggccgc ctcgacctcg gccggcgagg tgtagtcgag gaaggaggaa acgtcggcgc
      781 cggccaggaa cgaacccggc cgcgaactgc acagcacggc gccgaccaag cccgagtcct
      841 cgaccagctc ctcgaccgct tgcgagagcg cgcccagcag cgcggtgtcg agcaggttga
      901 cggggcgcgc aggctggtcg atgacgagca gcacgacgtc gccgcggcgc tcgacgcgca
      961 gtcccgagga ggcggtgtcg ggcggcttca cgccacgcct cccggcagac gcccgatcga
     1021 cagcgcgtgg ccgatgagca ggtagcggtc ggcgccctcg tcggagacat cgccgccgga
     1081 tgcgtcgccg ccggagagga gtccggggcc gctccggagc gtcgcgtccc agacgtgatc
     1141 gccgatcacg acgagagaca ggcgagcctc ggagccgccc gagaggctcg cggcgtgagc
     1201 tttttccgcg cgcataccgt ccattacagc aatatcgtat gcctcaagtg aaagctcgcg
     1261 tcgcgaagtt attcgagaac ccgttcttga ggcgggtcaa ctggcacctg agacggctgt
     1321 ccgatcagat ggaggcgggc ttcttcaggt ccctcctgat cgggctcgtc gtcatcctgc
     1381 tgttcatctc gctgatcgtg tgggcgttcg agacggatca cacgctcgcc gtcttcggcg
     1441 cgtcgttcta ctgggcggcg accaccgtgc tcggccaggg cgacgcctcg ttcgcgtccg
     1501 gaccggtcgg ctgggtgatg gggtggctgt tggggctgtt cggcgtcgcg atagtcgcga
     1561 ccatcaccgg cgcgctggtc gggttcatga tcgatttcct cctcaaggaa gggcagggca
     1621 tgggtgcatc cggttacagg ggccacatcg tcgtctgcgg ttggaacgcg acggctcgca
     1681 acctcatcga ggagctgaag gaggacgagt acggcggccg cgtcgtgctc gtctacgact
     1741 ccgagaagag ccccgccggc gacggcgtct acttcgtgcg cggctccgac acgtcggagg
     1801 ccgacctgaa acgcgccggg atcgagcacg ctcgctcggc catcgtgtgc ccttccgacg
     1861 gaacggacga ggccgacatg cactcgatcc tcgtcgtgct cgccatcgag tcggtcgccc
     1921 ccgacgtgcg caccgtcgta gaggtcaaca accccaagca cactcagcac ttccgccgcg
     1981 cccacgtcga cgagttcctc gtcacgtcgg tcctgtcctc gcacctgctg gccaggtcgg
     2041 cgatgtaccc ggggctctcc gagctcgtca ccgacatcgt ctcgggcggc gagggctcgg
     2101 aactctaccg ggtcgaactg ccggacgact gcgtcgacaa gccgatagac gaggtctcgg
     2161 cctggctacg caggacccac gccgccaccc tgctcgcggt ggcgcgcgag ggcgtcacgc
     2221 acaccaaccc ggctgccgac ttcaagctcg ccgccggcga cgacctggtg gtgctggcgg
     2281 agtcgctcgg caggctcagg ccgtacgagc gcgcggcggc gccggcctga ccacgctgtc
     2341 gcgagcgctg gccacgctca cggccccacc cgctcgagga gcatcgcgtg accctgccca
     2401 cccgcggcgc atgccgccac gagcgcgaac tgacctcccc cgacgttgag ccggtgcgcg
     2461 gccgtggcca cgaggcgcgc gccggtggcg ccgaacgggt ggccgaggga gacggaaccg
     2521 ccccacgggt tcaccttgtc catgcctacc tccggcgcga aggcgccgcc caggccgagc
     2581 ttctcccggc cgaaggcgtc gctctcgagg gcgcgcagga ccgccagcac ctggcccgag
     2641 aaggcctcgt ggagttcgac gacgtcgata tcgccccacg cgagcccgtt gcgagccagg
     2701 aggcggggga tggcataggc ggggccgagc aggagctcgt ccttggggtc ccgggccacg
     2761 aacaggaaat cgcgaacgcg caccaggccc gtccggccct cagccgccgc ccgctgctcg
     2821 cccatgacga gcacggccgc ggcgccgtcc gtcagctggc tggcgttgcc ggcggtcacg
     2881 gtgccgaacg gcttgacgaa ggccggcggc agcttcgcga gccgctcgag gctcgtgtcg
     2941 tcgcgcacgc cgttgtccgc cgccacgacc tgccccttcg ggggcacgct tacgggcacg
     3001 atctcctcgg ccaggaggtc gcggtgcgcc gccgcccctt ggtgcg
//
LOCUS       DAOQYE010000137         3042 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_34416_length_3042_cov_2.990961, whole genome shotgun sequence.
ACCESSION   DAOQYE010000137 DAOQYE010000000
VERSION     DAOQYE010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 3042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3042
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34416_length_3042_cov_2.990961"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="PKN52_03290"
     CDS             complement(<1..444)
                     /locus_tag="PKN52_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination/repair protein RecA"
                     /protein_id="HNQ98997.1"
                     /translation="MEDQRKKALDSALLQIERQFGRGAVMRLGAVPVGVGGVGVVSTG
                     SLSVDVALGVGGLPRGRIVEVYGPESGGKTTLALHVVAEAQAAGGVAAFVDAEHALDP
                     TYAAALGVDVGELLVSQPDTGEQALEIAELLTRSGAVDVIVVDSVA"
     gene            complement(562..2001)
                     /locus_tag="PKN52_03295"
     CDS             complement(562..2001)
                     /locus_tag="PKN52_03295"
                     /inference="COORDINATES: protein motif:HMM:NF013183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HNQ98998.1"
                     /translation="MNSVRSAEVIAVGTELLLGEVVDTNSARVAADLAEVGVDVYWSQ
                     RVGDNQARIVAALDAALDRSDLVVMTGGLGPTDDDLTREAIAALLGEAQTVDPGLEAW
                     LRRRFAANGREMPASNLRQAHVIPSSEILPNPVGTAPGWLVRLTRAGKERYIVTLPGP
                     PRELERMWLHEALPRLSLPNSKLFVRTFKTIGIGESLVAEMLGELTDQPNPSVATYAK
                     ADGVHVRVAAKADDLERAAVLAGPTVEKVAATLGDSVWGTDSDDLPRLVVSALRERGR
                     RLAIAEGGTGGVLTGYLVGADDGVDQLGRPKALNGKAATSGPASQRLGAVSGSVITCD
                     LETMRTLGVPIGLLQKLPYGAVEVVAALAAAVRAFFKADLGVANLGPCVPVLAASNDV
                     SIAEPDVGKLTRTQAGESPQAGPPGVLPPVPGAKPVGDDRTTTRVVIAIATESGTSVK
                     TLDLPPLGGPWQRERAAFTSLHLLRSALR"
     gene            2332..>3042
                     /locus_tag="PKN52_03300"
     CDS             2332..>3042
                     /locus_tag="PKN52_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase"
                     /protein_id="HNQ98999.1"
                     /translation="MTTSRAARSTSSTHDERPTTLRGLRGTRWEALAGRSVRDEIRTN
                     LVAALQRGQPLFPGVVGYDETVVPAVVNALLSRHNFILLGLRGQAKTRILRQLVTLLD
                     AEVPYIEGTELHDDPFAPLSMEGRELLAAQGDDTPIGWLSREQRYVEKLATPDTTVAD
                     IVGDIDPIKAARLGTRLGDERAVHFGLLPRANRGIFAINELPDLAGKVQVALFNVMQE
                     GDVQIKGYPMRLPLDVMLV"
BASE COUNT          519 a         1104 c          942 g          477 t
ORIGIN      
        1 cgccacacta tccaccacga tcacatcaac agcacccgac cgcgtcaaca actccgcgat
       61 ctccagggcc tgctcccccg tatccggctg cgacaccaac aactcaccca catccacacc
      121 caaagcagcc gcataagtcg gatccaacgc atgctcagca tccacaaacg ccgccacccc
      181 acccgccgcc tgcgcctcag ccaccacatg caacgcaaga gtcgtcttac cacccgactc
      241 cggaccataa acctcaacga tccgacccct aggcaacccc ccaaccccca acgccacatc
      301 aacactcaac gacccagtcg aaacaacccc aaccccacca accccaaccg gcaccgcccc
      361 caaccgcata accgccccac gcccaaactg acgctcaatc tgcaacaacg cagaatcgag
      421 agccttcttg cgctggtctt ccataagccc gcagcctccg ttcagatagg ggagggcctg
      481 tgacaggccg cccctagtgg cgcgctcttc tggctgcggg agcgttgggc cggtcgtgcc
      541 ggccacctcg ctcgccgagg ctcagcgcaa cgccgaccgg aggaggtgaa ggctagtgaa
      601 agcggcccgt tctcgttgcc acggcccgcc aaggggaggc aggtcgaggg tcttgacgga
      661 ggttccgctc tcagtggcta tcgcgatgac cacacgggtg gtcgttcggt cgtctcctac
      721 cggtttggcg ccgggtacgg gcggcaggac gcctggcggc cccgcttggg gcgactcgcc
      781 ggcctgcgtg cgcgtaagct tcccgacgtc tggttcagcg attgacacgt cgttcgaggc
      841 ggccaagacc gggacgcaag gccccaggtt ggcgacgcca aggtccgcct tgaagaacgc
      901 cctgactgcg gcggccagcg cggccactac ctcgaccgcc ccgtaaggca gcttctgcag
      961 tagtccgatt ggcacgccga gcgttctcat ggtttccagg tcgcatgtta tcacgctgcc
     1021 acttacggcc ccgaggcgct gggaagccgg gcccgaggtc gctgccttgc cgttgagcgc
     1081 cttgggccgc cccagttggt ctacgccgtc gtcggcgccg accaggtaac cggtcagcac
     1141 gccgcccgtg cctccttccg cgatggccag ccgccgtccc cgctcgcgca gcgccgagac
     1201 gacgaggcgt ggaaggtcgt cggagtccgt gccccagacg ctgtcgccga gcgttgccgc
     1261 taccttctcg acggtcggac ctgccagcac ggccgccctc tccaggtcgt ccgccttggc
     1321 ggccacccgg acatgcacgc cgtcggcctt cgcataggtg gcgacgctgg ggttgggctg
     1381 atcggtcagc tccccgagca tctccgccac gagcgactcg cctatcccga tcgtcttgaa
     1441 cgtgcgcacg aacagtttgc tgttgggcag cgacagcctg ggtagcgcct cgtggagcca
     1501 catgcgctcc aactcccgcg gcggcccggg gagcgtgacg atgtagcgct ccttgcctgc
     1561 cctggtgagg cgcacgagcc aaccgggcgc agtcccgacc gggtttggga ggatttcgga
     1621 ggacgggatg acgtgcgcct gacgcaggtt cgaggcgggc atctccctac cgttggcggc
     1681 gaagcgcctc ctcagccacg cttcgagacc tgggtcgacg gtctgcgcct cgcccagcag
     1741 tgctgcgatg gcctcccgcg tcaggtcgtc gtctgtcggg ccgagcccgc ccgtcatgac
     1801 cacgaggtcg gaacggtcga gggcggcatc gagcgcggcg acgatccgcg cctggttgtc
     1861 gccgacgcgc tgcgaccagt agacgtcgac gcctacctcg gccaggtcgg cggcgacgcg
     1921 cgcgctgttc gtatccacca cttcgccgag cagcagttcg gtgccaacgg ctatcacttc
     1981 ggcgcttctt acgctgttca tgagggagga taacagggcg agcagcgcgt gtagaacgag
     2041 tgacacgcca cacgcgcata gtgtagaatc cgttcggttc cttccccccg ggaaccaggg
     2101 agagggcctt gtgccctcca ccatctcctc tctttgaccc tgcgcccccc acgcagggtc
     2161 aatctttctc ggcagcttgt cgcacctaag aactggccgc gcctcgtgcc aggcgcgcct
     2221 cgcgctagac ggcttagtgc tgagcgcgcc gcgctaggcg cgcacctaga gagggtaggc
     2281 ggactcagct ccgccgacag gcgggctcat gtgccggtgc tagcattggc catgacgact
     2341 tcgcgggcag caagaagcac gtccagcacg cacgatgagc gcccgaccac cctccgcggg
     2401 ctccggggca cgcgctggga ggcgttggca ggcaggagcg tacgcgacga gatcaggacc
     2461 aacctcgtcg cggcactgca gcgcgggcaa cccctcttcc caggggtggt cggctacgac
     2521 gagacggtcg tgccggcggt ggtcaacgcg ctgctcagcc ggcacaactt catcctgctc
     2581 ggtttgcgcg gccaggcgaa gacccgcatc cttcggcagc tggtcaccct cctggacgcc
     2641 gaggtgccgt acatcgaggg caccgagttg cacgacgacc ccttcgcgcc cctgagcatg
     2701 gagggccgtg agctcctggc cgcccagggc gacgacacgc cgataggttg gctgtcccgt
     2761 gagcagcgct acgtggagaa gctcgccacg ccggatacga cggtggccga catcgtcggc
     2821 gacatcgatc cgatcaaagc cgcgaggctc ggcacgcggc tgggtgacga gcgcgcggtg
     2881 cacttcggcc tcctgccacg cgccaaccgc ggcatcttcg ccatcaacga gctcccggat
     2941 ctcgcaggca aggtgcaggt ggcgctcttc aacgttatgc aagaaggcga cgtccagatc
     3001 aagggctacc ccatgcgcct gccgctcgac gtcatgctgg tc
//
LOCUS       DAOQYE010000138         3036 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_34503_length_3036_cov_3.128816, whole genome shotgun sequence.
ACCESSION   DAOQYE010000138 DAOQYE010000000
VERSION     DAOQYE010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 3036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3036
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34503_length_3036_cov_3.128816"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="PKN52_03305"
     CDS             <1..271
                     /locus_tag="PKN52_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99000.1"
                     /translation="MWHLELVRNGVGWLYGGRKDAVEPLLAKGIAAAPGQVNLRVEYA
                     RALIELGKPAEARAQLELAATLPSRTASDDLEKADAAALLAGLDR"
     gene            487..2070
                     /locus_tag="PKN52_03310"
     CDS             487..2070
                     /locus_tag="PKN52_03310"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNQ99001.1"
                     /translation="MSLKRIATLVVALVISVVTAFGAAQSARQTTLVIGGDWGDLITL
                     DPGVSYEFTSTTILDNIYERLVKFNGDDLSVVVPGLAESWEIAARDGGGTSITFHLRD
                     ADFSTGRPVKASDVVYSFQRPILIQSPSAFLFTDVVGMDLDSVVAIDDKTVRLDMPEG
                     VNPSIVLNLLTFTTGGVMDQAEVEQHYEDGDYGQGWLNDNSAGSGPYMLERWDRSSQV
                     VLVTNPTYRDPGPVTRVIMRHMPESNAQRTALESGEIDIAWDYTPEAFQAAEAAGVLK
                     TYRTDTFQMSYLGMNSGPGAPFEDNRVRDAVRYAVDQQGIINSLLLGLGRPMQTIVPA
                     GLMGADTTVYYERNVAKAKELLAEAGVPNLSVEFLISTGACGGGVPCADLAAKIQSDL
                     AEAGITANIKQTTGGELYTIYRAQNAQLVMAAWSPDYPDPDGNATPLGSFPAGSIAWR
                     NVWDNAEAAQLASEAATTVDLTARQALYAKLNHLVATEGPYAMLYQPSKPIVTSPKVL
                     GFVRSAGGDVEFERISKLP"
     gene            2158..>3036
                     /locus_tag="PKN52_03315"
     CDS             2158..>3036
                     /locus_tag="PKN52_03315"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ99002.1"
                     /translation="MLSFLLRRVGSLIFVVWGVGFATFFISQVVPTDPAAAALGNNAR
                     PAQIAAYREKRGLDKPAWQQYLLYMQSLTKGDLGKSLRTQRPILNDLKDFFPATFELT
                     LGAMLFALLFGVPAGILAAVKQDRPVDLGVRTLALIGGATPVYWLAILALLLFHSKLG
                     WLPGPGRLDAFLLAPQRVTGILTLDALLARDWEVLYDALRHLLLPAAVLGAFSTALVA
                     RMVRSAMLEVLSQDFVRTAKAKGLPEGKVVLKHALRNAALPVLTVLGSLLGGLLTGAV
                     LTETIFSWPGIGSYATA"
BASE COUNT          478 a         1129 c          962 g          467 t
ORIGIN      
        1 catgtggcac ctcgaactcg tgcgcaacgg cgtcggctgg ctgtacggcg gccggaagga
       61 cgccgtcgag ccgctccttg ccaaaggtat cgccgcggcg cccgggcagg tcaacctgcg
      121 ggtcgagtac gcgcgcgccc tgatcgagct gggcaaaccc gcggaggcgc gggcccagct
      181 cgaactggcc gccacgcttc ctagccgcac cgcgtcagac gacctcgaga aggccgacgc
      241 cgccgcgttg ctcgcggggt tggaccggtg accgccgggc atcagccgcg agccgccgcg
      301 ctggcggggc agccaagacg gtacggcacg caagaaagcc ccgtgccgat gccggcccgg
      361 cctgaggccg acgccgcccg aggcgtggcg ctcgagcgcc gcatgcgtgt tactaccgaa
      421 ggtgtacact tcgcctaccc gtcaacccgc gcgggcaagc aatcgagtga tcgcaacgga
      481 ggcagcatga gtctcaagcg gatcgcaacc ttggtggttg cgctcgtcat atcggtggtt
      541 acggcgttcg gcgccgccca gtctgcccgc cagaccaccc tcgtgatcgg cggcgactgg
      601 ggcgacctca tcacgctcga cccgggcgtg agctacgagt tcaccagcac caccatcctc
      661 gacaacatct acgagcgcct cgtcaagttc aacggcgatg acctctcggt cgtcgtcccc
      721 ggcctggcgg agtcgtggga gatcgcggcg cgcgacggcg gcggcaccag catcacgttc
      781 cacctgcgcg acgccgactt ctcgaccggt cgccccgtca aggcttccga cgtcgtctac
      841 tccttccagc gccccatcct catccagagc ccgtcggcgt tcctgttcac cgacgtcgtc
      901 ggcatggacc tcgactccgt ggtggccatc gacgacaaga ccgtccgcct cgacatgccg
      961 gagggcgtga acccgagcat cgtcctcaac ctgctcacgt tcaccaccgg cggcgtcatg
     1021 gaccaggccg aggtcgagca gcattacgag gacggcgact acggccaagg ctggctgaac
     1081 gacaactccg ccggctccgg cccgtacatg ctcgagcgtt gggaccgcag ctcccaggtc
     1141 gtgctcgtca ccaaccccac gtaccgcgac ccgggcccgg tcacgcgcgt gatcatgcgc
     1201 cacatgcccg agtccaacgc tcagcgcacc gcgctcgagt ccggcgagat cgacatcgcc
     1261 tgggactaca cgccggaagc cttccaggcc gctgaggccg ccggggtcct caagacctac
     1321 cggaccgaca cgttccagat gtcctacctg ggcatgaact ccggccccgg cgcgccgttc
     1381 gaggacaacc gcgtccgcga cgccgtgcgt tacgccgtcg accagcaggg catcatcaac
     1441 agcctcctgc tcggcctggg ccgcccgatg cagaccatcg tcccggccgg cctgatgggc
     1501 gccgacacga ccgtctacta cgagcgcaac gtcgcgaagg ccaaggagct gctcgccgag
     1561 gccggcgtgc cgaacctgag cgtcgagttc ctcatctcga ccggcgcctg cggcggtggc
     1621 gtaccctgcg ccgacctcgc cgcgaagatc cagtccgacc tcgccgaggc cggcatcacc
     1681 gccaacatca agcagacgac gggcggcgag ctctacacga tctaccgtgc ccagaacgcc
     1741 cagctcgtca tggccgcgtg gagccccgac taccccgatc ccgacggcaa cgccacgccc
     1801 ctcggcagct tccccgccgg ctccatcgct tggcgcaacg tgtgggacaa cgccgaggcg
     1861 gcgcagctcg ccagcgaggc cgccacgacg gtcgacttga ccgcccgcca ggcgctctac
     1921 gcgaagctga accacctcgt cgcgaccgaa ggcccgtacg ccatgctcta ccagccttcc
     1981 aagcccatcg tcacgtctcc caaggtgctc ggtttcgtgc gcagcgccgg cggcgacgtc
     2041 gagttcgaac gtatcagcaa gctcccctga gcaaaggggt tagagcttga tccgagaagc
     2101 acgaggagag ggcctgggtc accgggccct ctctgagcat agccgcgagg cgagagcgtg
     2161 ctgagctttc tcctgcgccg ggtcggctcg ctcatcttcg tcgtctgggg cgtcgggttc
     2221 gcgactttct tcatctccca ggtcgtgccg accgaccccg ccgccgcggc gcttggcaac
     2281 aacgcgagac cggcacagat cgcggcctac cgggagaagc gcggcctcga caaaccggcc
     2341 tggcagcagt acctgctgta catgcagtcc ctcaccaagg gcgacctcgg caagtcgctg
     2401 cgcacgcagc ggcctatctt gaacgacctc aaggacttct tcccggccac gttcgagctc
     2461 acgctgggcg ccatgctctt cgccctgctc ttcggggtcc cggccggcat cctcgccgcc
     2521 gtgaagcagg accggccggt cgacctcggc gtcaggacgc tcgccctcat cggcggcgcc
     2581 acgcccgtct actggttggc catcctggcg ctcctcctct tccactcgaa gctcgggtgg
     2641 ttgcccgggc ctggtcgtct ggacgccttc ctgttggcgc ctcagcgtgt gacgggcatc
     2701 ctcacgttgg acgccctgct ggccagggac tgggaagtgc tgtacgacgc cctgaggcac
     2761 ctgctcctgc cggcggccgt gctcggcgct ttctcgacgg ccctcgtggc ccggatggtg
     2821 cgtagcgcca tgctcgaggt cctctcgcag gacttcgtcc ggacggccaa ggccaagggc
     2881 ctgcccgagg gcaaggtcgt gctcaagcat gcgctgcgca acgcggcgct gccggtgctg
     2941 acggtgctcg ggagcctcct gggcgggttg ctcacgggcg cggtgctcac cgagacgatc
     3001 ttctcgtggc ccggcatcgg ctcgtacgcc accgcg
//
LOCUS       DAOQYE010000139         3027 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_34646_length_3027_cov_3.174293, whole genome shotgun sequence.
ACCESSION   DAOQYE010000139 DAOQYE010000000
VERSION     DAOQYE010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3027
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34646_length_3027_cov_3.174293"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..991)
                     /gene="tgt"
                     /locus_tag="PKN52_03320"
     CDS             complement(<1..991)
                     /gene="tgt"
                     /locus_tag="PKN52_03320"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HNQ99003.1"
                     /translation="MATPAFVAVGTQATVKSLAPEAVAATGTGLLFANTYHLYLRPGA
                     DLVARHGGLHRFMRWDGPILTDSGGFQVFSLGASIEHGVGKIGNIFPGEDKDRSRRRL
                     AAGSGMVKVDEEGVVFKSHIDGSKHRFDPETSISVQRLLGADIVLAFDECTSPLHDEA
                     YTRRSLERTHRWAVRSLAAFQNTASLHGYRQALYGIVQGGAFRDQREDSAGFVGALAF
                     DGIAIGGNLGSTREDMHAILDWTVPLLPIGKPRHLLGIGDVESIFEAVARGIDTFDCV
                     MPTRNARTGTLLVRRDLDGGAGGGRLNITNARFQSDLGPVQAGLRGQVGQGRPP"
     regulatory      1411..1495
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     regulatory      1585..1667
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            1748..2737
                     /locus_tag="PKN52_03325"
     CDS             1748..2737
                     /locus_tag="PKN52_03325"
                     /inference="COORDINATES: protein motif:HMM:NF014237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HNQ99004.1"
                     /translation="MIRLTERVRNLGGFAPYGKMVPVLLLGALMLSACGSAQEGAVSA
                     TRSAPALEATTMFGAFTYGGVWRGMDPVYELEADLGRRLDVVHWFTNWETPYYPELVA
                     MASQGGRRPMISWQSYDLSVRDIAAGKYDGYITDWARGAATAPGLLYVRPFPEMNGDW
                     TTWNGDPEGLRLAWRHIVDIFDSVGATNVRWVFSPNVTDSPRVPENAMELYYPGDDYV
                     DVLALDGYNWGEVLPSVGWRSFEAIFRAGYDRITRLGDQPIWIAELASSNVGGDKSDW
                     VREMFATTGFERIEALVWFNENKEADWRIDSDVATLHAFRQVLGAGTTVAAVR"
BASE COUNT          490 a         1015 c         1037 g          485 t
ORIGIN      
        1 gcggcgggcg cccctgccca acctgccccc ggaggcctgc ctggacgggg ccgaggtcgc
       61 tctggaagcg cgcgttggtg atgttgaggc gaccgccgcc cgcaccgccg tccaggtccc
      121 gcctgacgag cagcgtgccg gtccgggcgt tgcgggtagg catgacgcag tcgaacgtat
      181 cgatgccgcg ggcgacggcc tcgaagatgc tctcgacatc accgatcccg agcaggtgcc
      241 gcggtttgcc gatcgggagg agcggcaccg tccaatcaag gatggcgtgc atgtcctcgc
      301 gcgtcgagcc gaggttcccg ccgatcgcga tgccgtcgaa agccagcgcg ccgacgaagc
      361 cggcgctgtc ctcgcgctgg tcgcggaaag cgccgccctg gacgatgccg tacagggcct
      421 ggcggtagcc gtgcagcgaa gcggtgttct ggaaagccgc caagcttcgc accgcccagc
      481 ggtgcgtgcg ctcgagggag cgccgcgtat acgcctcgtc gtgcaagggg ctcgtgcact
      541 cgtcgaaggc gaggacgata tcggccccga gcagccgctg gacggagatc gacgtctccg
      601 ggtcgaaccg gtgcttggag ccgtcgatgt ggctcttgaa caccaccccc tcctcgtcga
      661 ccttgaccat cccgctgccg gccgcgaggc gcctgcggct ccggtccttg tcctcgccag
      721 ggaagatgtt gccgatcttg ccgaccccat gctcgatcga cgcgccgagc gagaagacct
      781 ggaagccgcc ggagtcggtg aggatcggac cgtcccaccg catgaagcgg tgaaggccgc
      841 cgtggcgcgc cacgaggtcg gcgccgggcc gtaggtacag gtgatacgtg ttggcgaaca
      901 ggagaccggt gcccgttgcg gcaacggcct ccggggcgag gctcttgacg gtcgcttggg
      961 tgccgaccgc gacgaacgcc ggtgtcgcca cgtctccgtg cggcgtgcgg aggcgtccga
     1021 gccgcgcgcg ccctccttgg gcgcgcccta cttgggttcg tcctactcga ccgcgccctg
     1081 gtagggcgca cccgcgctcg gtcaacacgt cgaacgcgaa cgtgtcctcg gttgcggtcg
     1141 tcttcgttat ggggtcctgt ccatgcgagc tgtccatgcg agctggcccg tcgggcccgt
     1201 catgttacca gagcgcctcg gatggccgcg ttccccacgt cggggccggc caagggcacg
     1261 ctccgggcgg cgggcgggca agcggcggac aacgggtcgg cgaagggcgg gcaaaggtcg
     1321 tgcgacgggc ggcgagcagc cggccatggg tgattacttc acgcgcatga gcgccggatg
     1381 tgctatagtc cccttcggga agtcgaccac ttcccgcttg gacgctgaga aacctggctc
     1441 gttatttggg cgcttgagtc aggtggagcg agtagcgcaa ccggccaggt gagtgtcccc
     1501 tcatctaaaa gtgccgtcaa cgtccgagcg cgggtcctga ccagacccgc cgcccgagtc
     1561 gttcgggcac ccgagcctca cggctcggac ttggaagcga tgaagcctgt tctgcatctg
     1621 ggcgcttgga acaggtggag ctagtagcgc aaccggccaa gggacgcatg ggtctagacg
     1681 gaaccgttcc gcactagcca gcagccgggc gagcgcctta ggacgttcaa ggacgtccgg
     1741 ggacttgatg atccggctga ccgaacgagt acgcaacctg ggagggttcg caccatatgg
     1801 gaagatggtg cctgtcctgc tcctcggcgc gctcatgctg agcgcgtgcg ggagcgccca
     1861 ggagggagcc gtctcggcga ctcgttctgc gccagctttg gaagcgacca ccatgttcgg
     1921 cgccttcacc tacggtggcg tgtggcgcgg catggacccc gtctacgaac tcgaagccga
     1981 cctcggtcgg cgcctcgatg tcgtccactg gttcaccaac tgggagacac catactaccc
     2041 cgagctcgtg gcgatggcgt cccaaggcgg ccgcaggccc atgatcagct ggcagagcta
     2101 cgacctgagc gtgcgggaca tcgccgccgg caagtacgac ggctacatca cggattgggc
     2161 gcgcggcgcg gccaccgccc ccggcctcct ctacgtgcga cccttccccg agatgaacgg
     2221 cgactggacg acgtggaacg gcgatccgga agggctgagg ctcgcctggc gccacatcgt
     2281 cgacatcttc gactccgtcg gggccacgaa cgtccgctgg gtgttcagcc cgaacgtcac
     2341 ggactcccca cgcgtgcccg agaacgccat ggagctctac taccccggcg acgactacgt
     2401 cgacgtcctg gcgctcgacg gctacaactg gggcgaggta ctgccttccg tcggttggcg
     2461 ctcgttcgag gccatcttcc gcgccggtta cgaccgcatc acgcgcctgg gcgaccagcc
     2521 gatctggatc gccgaactcg cgagttcgaa cgtcggcggc gacaagagcg actgggtccg
     2581 cgagatgttc gcgacgacgg ggttcgaacg tatcgaggcg ctcgtctggt tcaacgagaa
     2641 caaggaagcc gactggcgca tcgactcgga cgtagctacc ctgcacgcct tccgtcaggt
     2701 cctcggcgcc ggcacaacgg tcgcggcagt ccgctgaccc ggctcgcgaa cgagcgtcat
     2761 ctgagaagcc gatgagctag cgggcccttg ggttaagaac gcgagcgttg cgtctcagac
     2821 gagcctaatg gtatccatat gtgaaatgtc acagcgctgg ccccggccag gcgcacgcgg
     2881 gaagtccctt gaccgacagc cgtcccaccc gacgagttca gaccggcgca gacgagcgtc
     2941 agactgccca ccagccgtca gccgggctgg ccttggcgct gctcgttacc gtcctcgcac
     3001 tcgtcctggg cgcctgctcc gaaccgc
//
LOCUS       DAOQYE010000140         3025 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_34680_length_3025_cov_2.438384, whole genome shotgun sequence.
ACCESSION   DAOQYE010000140 DAOQYE010000000
VERSION     DAOQYE010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 3025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3025
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34680_length_3025_cov_2.438384"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(249..434)
                     /locus_tag="PKN52_03330"
     CDS             complement(249..434)
                     /locus_tag="PKN52_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99005.1"
                     /translation="MFSSIEQIHRSTLRPATGSALRRAGYFDGERAPRRDHYWANQPT
                     TVNAVTRSWRSSSRRSR"
     gene            717..>1944
                     /locus_tag="PKN52_03335"
     CDS             717..>1944
                     /locus_tag="PKN52_03335"
                     /inference="COORDINATES: protein motif:HMM:NF021665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G8 domain-containing protein"
                     /protein_id="HNQ99006.1"
                     /translation="MTILSPLRAAALFTFCVLTLSACGALNTNTPPSGCAGTACCMAA
                     HHAATGATAGTAGAAHSHSLMNMLGVAGAGSALGLTPAAEPAPTRVWSDSAGWPGGVL
                     PAEGDDVVIGAAEVVLLDVSPPRLGGLTIEGALVFDRQNLALAADHILVHGALHVGSE
                     AEPFLQRAVITLTGERRADDGCLGGNYLALVGGSLELYGDPTGTSWTRLASTAAAGAS
                     SIEVEDATGWSAGDMLAIASTDYYADQTVAGVRRDAQVEERRVTAVDGNRLQLDSPLA
                     YEHYGEAQTLAAGFPTTVLESRAEVVRLTRNVTVQSREETADPGNPDYLYGGHVMAMG
                     ASSVRLDSVELTRMGQAGILLRYPVHWHLMGDNGVGSFVRNSTLHDLYNRCVTIHGTN
                     GVVLDGNAAYNTFGHCY"
     assembly_gap    1945..1954
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1955..>3025
                     /locus_tag="PKN52_03340"
     CDS             <1955..>3025
                     /locus_tag="PKN52_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99007.1"
                     /translation="GAESRNVLRGNLTRQVREPAEKNALLPSDTGYLGPAAFWITNPG
                     NDLIGNVAASSDGSGFWFALPEHPTGPSYNVFDGANTWPRRTPLGTFDGNMAHSNMQD
                     GLHVDRGPRASDLSPETTYYDPHVDPADRDSASVQAVFSGYVAYKHRSTAAWFRGANA
                     VLRGALLADNAIGVTFASRASGLEDSVIVGETANRGSPRNWETTGVDGRTVPRHWDAD
                     FAIRGFEFYDGPVFVRNSYFAGFEPNSLRAASAISALDYTSFSMSPTSFAQGLGFDAA
                     TNRVRFETRDMSTYDAKTTDSGEDGYRSAVFEDVDGSVTGTAGRYVTVTNPVLAAPTC
                     TYRADWNANVCDGRYVALTFRDD"
BASE COUNT          442 a         1101 c         1032 g          440 t
ORIGIN      
        1 cgccgagggg aacgtcaggc ggcgcgcgat cggcgccgtc gggatggtgg tcggggtcac
       61 gctcctggcg ctggcgtgag cagtgccggg ctcgtcgccc gtcagacagg aaacggctcc
      121 ttaccggaac cgactcccta tgggttcggc gatgtgcgcg gtcgcccacg gacctcgcgg
      181 tggctcaaag gttcgggcgg cgccccgcta ggaggcgccg cccgtctggt cggcctgccg
      241 cggaccgctc agcggctgcg acggctgcta ctacgccagg aacgcgtgac cgcgttgacc
      301 gtcgtgggct ggttggccca gtagtggtcg cggcgcgggg cgcgctcgcc gtcgaagtag
      361 ccggcgcgcc gtagggcgga gccggtggcg gggcggaggg tggaacggtg gatctgctcg
      421 atgctggaga acatggtctc ctccttcgtg ggagttcggc gccccggcgg tcgccgggca
      481 gcgcgctgct tctagaccct ccggttgggg gtccgatcag gttcgctagt gtcggtggta
      541 gcgcgggcgc ggcccgcgtt cacgtctccc cgaggcccgt tgactgagag ggcgggaccc
      601 gggatgcctc ggagcctgac ccgaagcggc aagcatgcta ccagattcac aagcgctgtg
      661 agaagccccg tactacttca catctgaatg ggggtccgac cccctaccct tggccgatga
      721 ccatcctgtc ccccctcagg gcggccgccc ttttcacgtt ctgcgtcctc acgctctcgg
      781 cctgcggggc cttgaacacc aacacgccac cttccggatg cgccggcacc gcatgttgca
      841 tggccgcgca ccatgccgcc accggcgcta ccgcgggcac tgccggagcc gcgcactcgc
      901 attcgctcat gaacatgctg ggcgtggccg gcgccggcag cgccttaggc ctcacgccgg
      961 ccgccgagcc ggcgccgaca cgcgtctggt cggacagcgc cggttggccg ggcggcgtcc
     1021 tccccgccga gggcgacgac gtcgtcatcg gcgcggccga ggtcgtgctc ctggacgtct
     1081 cgccgccgcg cctcggcggc ctgaccatcg aaggcgcgct cgtgttcgac cgccagaacc
     1141 tcgcgctcgc ggccgatcac atcctcgtcc acggggcgct ccacgtcggc tcggaggcgg
     1201 agccgttcct ccagcgcgcc gtgatcaccc tcacgggcga gcggcgagcg gacgacggct
     1261 gcctcggcgg caactatcta gcgctcgtag gcggaagcct ggagttgtac ggcgacccga
     1321 ccggcaccag ttggacgcgc ctggcgtcca cggccgcggc cggcgcgtcc agcatcgagg
     1381 tggaggacgc taccggctgg agcgccggcg acatgcttgc catcgcttcc accgactact
     1441 acgccgacca gacggtcgcc ggcgtacgcc gcgatgcgca ggtggaggag cggcgggtca
     1501 cggccgtcga cggcaaccgg ctccagcttg actccccgct ggcgtacgag cattacggcg
     1561 aggcccagac cctggcggcc ggcttcccga ccacggtcct cgagtcgcga gcggaggtcg
     1621 tgcgcctcac gcgcaacgtc acggtgcaga gccgcgagga gaccgccgac cccggcaacc
     1681 ccgactacct ctacggcggg cacgtcatgg ccatgggcgc cagcagcgtg cggctcgact
     1741 ccgtggagct gacgcgcatg ggccaggccg gcatcctcct gcgctacccc gtgcactggc
     1801 acctgatggg cgacaacggc gtgggcagct tcgtccgcaa ctcgaccctg cacgacctct
     1861 acaaccgttg cgtgaccatc cacggcacga acggcgtggt gctcgacggc aacgcggcct
     1921 acaacacgtt cggccactgc tactnnnnnn nnnnggcgcc gagagccgca acgtgctgcg
     1981 cggcaacctc acgcggcagg tgcgcgagcc ggccgagaag aacgccctcc tcccgtcgga
     2041 caccggctac ctcggtccgg cggcgttctg gatcaccaac ccgggcaacg acctgatcgg
     2101 caacgtcgcc gcctcctcgg acggttccgg cttctggttc gccttgccgg agcacccgac
     2161 cggcccgtcc tacaacgtct tcgacggcgc caacacctgg ccgcggcgca cgccgctcgg
     2221 cacgttcgac ggcaacatgg cgcactcgaa catgcaggac ggcctgcacg tggatcgcgg
     2281 gccgcgcgcg agcgacctgt cgccagagac gacctattac gacccgcacg tcgacccggc
     2341 cgaccgcgac tccgcctccg tgcaagcggt gttctccggg tacgtcgcct acaagcaccg
     2401 gagcactgcg gcctggttcc gcggcgcgaa cgccgtgctg cggggcgcgc tgctggcaga
     2461 caacgcgatc ggcgtcacgt tcgcctcccg cgcttccggg ctcgaggaca gcgtgatcgt
     2521 aggcgagacg gccaaccgcg gctcgccgag gaactgggaa acgaccgggg tcgacgggcg
     2581 caccgtgccg cggcactggg acgccgattt cgccatccgc ggtttcgagt tctacgacgg
     2641 gcccgtgttc gtgaggaact cgtacttcgc ggggttcgag ccgaactccc tgcgcgcggc
     2701 cagcgcgatc agcgccctcg actacacgtc gttctccatg agcccgacct cgttcgcgca
     2761 ggggctcggc ttcgacgccg cgacgaaccg cgtgcgcttc gagacgcgcg acatgagcac
     2821 ttacgacgcc aagaccaccg actcgggtga ggacggctac cgcagcgccg tcttcgagga
     2881 cgtcgacggc agcgtgaccg gcacggccgg ccgttacgtg accgtcacca accccgtgct
     2941 ggccgcgccg acctgcacct accgggcaga ctggaacgcc aacgtgtgtg acggacgtta
     3001 cgtcgccctc acgttccgcg acgac
//
LOCUS       DAOQYE010000141         3015 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_34848_length_3015_cov_3.634459, whole genome shotgun sequence.
ACCESSION   DAOQYE010000141 DAOQYE010000000
VERSION     DAOQYE010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 3015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3015
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34848_length_3015_cov_3.634459"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1212)
                     /locus_tag="PKN52_03345"
     CDS             complement(<1..1212)
                     /locus_tag="PKN52_03345"
                     /inference="COORDINATES: protein motif:HMM:NF012361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNQ99008.1"
                     /translation="MPAIFSVDLTGKVMPEVRLVTDAAVTGPGPYTGVWFKDADAAIT
                     ADLDVAGRMFASGTIRHAYPFNWRDGKPLMNVAKRSWYIRTTAVKELLLANNDRIGWH
                     PDHVRTGRFGKWLENNIDWALSRERYWGAPMPVWETADGGKKVCVGSVAELERLTGRD
                     LKDLDLHRPYVDEITFELDGETYRRVPYTVDVWFESGAMPYAQWHYLGAASSAEAQER
                     LAANFPADYICEAVDQTRGWFYSLHALATLLTDPGDPATGRRPGPLAHLSEPTPAFKN
                     VNVLGHIVDEKGEKMSKSKGNTVNPWTILDAQGADALRWYLYSASPPEATKRFSAGLV
                     DETLRDFFLTLWNVYGFFVLYANLEEPDLASAPRPAERPLVDRWLVSRLNAVVAGVTA
                     ALEDFDPTTASR"
     gene            complement(1185..2291)
                     /locus_tag="PKN52_03350"
     CDS             complement(1185..2291)
                     /locus_tag="PKN52_03350"
                     /inference="COORDINATES: protein motif:HMM:NF012361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNQ99009.1"
                     /translation="MFDEVSSNVDFPALEKRVIQLWQERDVFRRSIDRPAERGDWVFF
                     EGPPTANGKPGVHHVSARAFKDLFPRFKTMQGYRVGRKGGWDTHGLPVEIAIEKRLGF
                     TNKSQIDEYGIAEFNALCRADVFSNIQDWNAMTERIGFWIDLADPYITYTNEYIESCW
                     WIMKDLFDRGLLVEDYKTTWHSPSSNTTLASHEVSLGYQEDVEDPSIYPKFPAVTSDL
                     VARGVVEPSEARPVNFLAWTTTPWTLAANSGLAVRADAEYALVEAPLRRKEPGGPREL
                     YLLARELAPQVFEAGTYEVLRSVPGATLAGVGYRPLLVGRPGDGADMGSAYRVVTDDF
                     VSLTDGTGIVHVAPSYGDLDVGRASPCRPSSPST"
BASE COUNT          487 a          974 c         1062 g          492 t
ORIGIN      
        1 gcggctcgcc gtcgtcggat cgaagtcctc cagcgcggcg gtcacgccgg ctacgacggc
       61 gttcaagcgc gagacgagcc agcggtcaac gagcgggcgc tcggccgggc gcggggcgga
      121 ggcgaggtcg ggctcctcga ggttggcgta gagcacgaag aagccgtaga cgttccagag
      181 cgtcaggaag aaatcgcgta gcgtctcgtc cacgagcccg gcgctgaagc gcttcgtcgc
      241 ctcgggcggc gaggcggagt agaggtacca gcgcagcgcg tcggcgccct gagcgtcgag
      301 gatggtccac gggttgaccg tgttgccctt cgacttggac atcttctcgc ccttctcgtc
      361 cacgatgtgg ccgagcacgt tgacgttctt gaacgcggga gtcggttcgc tcaagtgtgc
      421 gaggggcccg ggtcggcggc ccgtggccgg gtcgccgggg tccgtcagga gcgtggcgag
      481 cgcgtggagg gagtagaacc agccacgcgt ctggtcgacg gcctcgcaga tgtagtccgc
      541 ggggaagttg gcggccaggc gctcctgcgc ctcggcgctc gaggcggcac caaggtagtg
      601 ccactgcgcg tacggcatgg cgccggactc gaaccacacg tcgaccgtgt aaggcacacg
      661 ccggtaagtc tcgccgtcga gctcgaaggt gatctcgtcg acgtaggggc gatgaaggtc
      721 gaggtccttc aggtccctgc cggtgagtcg ctcgagctcg gcgaccgacc ctacgcatac
      781 cttcttgccg ccgtccgccg tctcccagac cggcatcggg gcgccccagt agcgttcgcg
      841 gctcaaggcc cagtcgatgt tgttctcgag ccacttgccg aagcggccgg tgcgcacgtg
      901 gtcagggtgc cagccgatgc ggtcgttgtt ggcgagcagg agctccttga cggccgtcgt
      961 gcggatgtac cagctgcgct tggcgacgtt catgagcggc ttgccgtcgc gccagttgaa
     1021 cgggtaggcg tgacggatcg tgccggaggc gaacatccga ccggcaacgt cgaggtcggc
     1081 ggtgatggcc gcgtcggcgt ccttgaacca gaccccggtg tacggtccag ggcccgtcac
     1141 ggccgcgtcg gtaacgaggc gcacctcggg catcaccttg ccggtcaggt cgacggagaa
     1201 gatggccggc aaggtgatgc gcgaccgacg tccaggtcac cgtagctcgg tgcgacgtgc
     1261 acgatacccg tgccgtccgt aagactcacg aagtcgtccg tcaccacgcg gtaggcggag
     1321 cccatgtcgg cgccgtcgcc ggggcgcccg accagaaggg gccggtagcc gaccccggcc
     1381 aaggtggcgc ctggcacgct gcggagcact tcgtaggtcc ccgcctcgaa gacctgtggg
     1441 gcaagctcgc gcgcgaggag atagagctcg cgggggccgc cgggctcctt gcggcgcagc
     1501 ggcgcctcca cgagggcgta ctccgcgtcg gccctcactg ccaggccgga gttcgcagcc
     1561 agcgtccatg gcgtcgtcgt ccacgcgagg aagttgacgg gccgcgcctc tgaaggctcc
     1621 accacgcccc gggcgacgag gtcgctcgtg acggcgggaa acttcggata gatggacggg
     1681 tcctccacgt cctcctggta gccgagtgac acctcgtggg aggcgagcgt cgtgttggac
     1741 gacggactgt gccaggtcgt cttgtagtcc tccacgagca gaccgcggtc gaacaggtcc
     1801 ttcatgatcc accagcacga ctcgatgtac tcgttcgtgt aggtgatgta agggtcggcc
     1861 aggtcgatcc agaagccgat ccgctcggtc atggcgttcc agtcctggat gttgctgaac
     1921 acgtcggcgc ggcatagggc gttgaactcg gctatgccgt actcgtcgat ctggctcttg
     1981 ttggtgaagc cgaggcgttt ctcgatggcg atctcgaccg gcaggccgtg agtgtcccaa
     2041 ccgcccttgc ggccgacgcg gtagccctgc atcgtcttga agcgcgggaa caggtccttg
     2101 aaggcgcggg cgctgacgtg gtgcacgccc ggcttgccgt tcgccgtcgg cgggccctcg
     2161 aagaacaccc agtcaccccg ctcggcgggg cggtcgatgc tgcgccggaa gacgtcccgc
     2221 tcctgccaga gctggatgac acgcttctcc agcgcgggga agtcgacgtt tgagctgacc
     2281 tcgtcgaaca tacggctcac ctcatgcccg agatcagcgc ctggcgcacg acaagaggcg
     2341 ccccagacac gacgtgttgg ggcgcctcgg gagcgcggta ccacccaacc gtgccgttcg
     2401 ccgtcgccgg cgtcggcctc gccgaccgcc gctgctgaag cgacggcccg gtgctgtcac
     2461 gggcaccacc cggctccgtc taccacctac tggacgttcc gcggcccagg gcaggcttcg
     2521 gggagcggct cggggaggat acctgacgcg ccgaccggcc tccgggctct caccgttccc
     2581 ggatcgctgt gggcggcccc atgcgcgagg actgttcccg tcgtcgcctt ggccttgcga
     2641 ggagcggtga cgttggtcac gtcctctgag cgcgtgacac tttacaacca gcagtgtcgg
     2701 cgctagactc aaacgtagca gcaggcatga ggcacgtcaa taggttctgt tgctgcccct
     2761 aagcacaacc aggcgcttcc tggtacggca accaagcaag caaccgaccg tcgacttgag
     2821 cgaccgttcg cctccgcggc gccaaggagc gacccctcga aaggcgatac gcctccgtgc
     2881 ggcagcgtgg tggacaggtc gaaggccgga agcgcccgga tcctgaagga aacgtgtcgt
     2941 ggttcacgcg tcggatagaa cgcagaccgt gtagccggaa ggtgcctaag gagatggcat
     3001 gagcccgaaa gaaga
//
LOCUS       DAOQYE010000142         3005 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_35014_length_3005_cov_2.457627, whole genome shotgun sequence.
ACCESSION   DAOQYE010000142 DAOQYE010000000
VERSION     DAOQYE010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 3005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3005
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35014_length_3005_cov_2.457627"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(247..996)
                     /locus_tag="PKN52_03355"
     CDS             complement(247..996)
                     /locus_tag="PKN52_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNQ99010.1"
                     /translation="MIKVIAIGDVHGQWPELWRVLRAASAATATLEPTQAVMEGRYRV
                     VCVGDLVHYKDVRDYANAVGVEPYDPSDPDHLRRAAKAQIRELYRFKRYVDQSEGNVS
                     VIIGNHDEAAFDHRYELSTRGGMNHLEFDSSKGGVELPDDLKEWFASMPREVVFHGVQ
                     FAHAGPLPGMQLFDDFFYHDPDTKSWWFNKPELVKQAGHRFGVYGHTVMKDGVYLDKE
                     NGFAMIDALGKLQYLELLLDEDRLDLSVMQL"
     gene            complement(1031..1810)
                     /locus_tag="PKN52_03360"
     CDS             complement(1031..1810)
                     /locus_tag="PKN52_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99011.1"
                     /translation="MQPELSATLAPPLASDALRWHVAEVEPGAARARLEPYATRAAIA
                     ERLDVACGRLGWSFSLAPLGASALVANLTVRGATRSDVIALCVPSGGVPLPDALARSG
                     EFALARCAAQFGLRLPYVSDDPSYWVDFDAEAGEALFEPEPVCASAGPGPLTAQAGQG
                     VVAATAAVAAATAAAAAIAAVEEADSERATEPPPQKTGSASGGHEVIERLIERLNAEG
                     LGREAAKLVVRYGGYGRTPEESRELYRQLRSILLQREAQPA"
     gene            1930..>3005
                     /locus_tag="PKN52_03365"
     CDS             1930..>3005
                     /locus_tag="PKN52_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99012.1"
                     /translation="MSKPRAVPNRAGKSPSPSPAGTGRVGSFLAIIAASVLTLGAALA
                     QTAPVVGLDAYDFEVGQTLNVSGDRLTPGSAYRIAVGPVGGAATVSVVQADANGSIRY
                     QTVLEEPGDHGVSIDGDDIHAVFTISVRGPDAAPESAQEAGPEPAEEASQGGGEAPAT
                     QDAQPAQETPETPSQQGATGATAAPTQPNQEPPAANEPGGLAQPSPVQTYPTARLVGN
                     EVQGAQGEEIVWRLSFPSGSGETAGLVQSGLDVYVGHGNHVLRVDARTGKITKRHRLP
                     AQVTALQGRGGALNATVTYSTGAEGSFAILETSQDPNEPFDDDPALYGWLRKEAAGGD
                     PQERLQNDETNPWLYLEAARAATPG"
BASE COUNT          505 a         1066 c         1033 g          401 t
ORIGIN      
        1 cgacctcgtc gacagctaca agcgccgcac gctggtcttg cgcaacggcg agctcgtccg
       61 cgacgacgcg cggggcggct actcgctctg acgtcctgac cgcggtctgc gcccctgatc
      121 gcggtcaggg gcgcccgacg aacccgcgcg aagtcggggt cgggccgggc ccatacgtcg
      181 gatcgcgcct cgcgctaccc gaagctcgac atgccggccg gctagagctg gatctagaac
      241 tgtatcttag agttgcatga cgctcaggtc gagccggtcc tcgtccagca gcagctcgag
      301 gtactggagc ttgccgagcg cgtcgatcat ggcgaagccg ttctccttgt caaggtagac
      361 gccgtccttc atgaccgtgt gaccgtagac cccgaagcgg tggccggcct gcttgacgag
      421 ctcgggcttg ttgaaccacc aggacttcgt gtcggggtcg tgatagaaga aatcgtcgaa
      481 cagctgcatg cccggcagcg ggcccgcgtg ggcgaactgc accccgtgga acactacctc
      541 gcgcggcatg gatgcgaacc actccttgag gtcgtccggg agctcgacac cgcccttcga
      601 cgagtcgaac tccaggtggt tcatcccgcc gcgcgtcgag agttcgtacc ggtggtcgaa
      661 ggcggcctcg tcgtggttgc cgatgatgac ggagacgttg ccctcgctct ggtcgacgta
      721 cctcttgaag cggtatagct ccctgatctg cgccttggcg gcacggcgca ggtggtcagg
      781 gtcggacgga tcgtaaggct cgacgccgac cgcgttggcg tagtcgcgaa cgtccttgta
      841 gtgcaccagg tcgccgacgc acacgacccg ataacgacct tccatgacgg cctgcgtcgg
      901 ctcgagcgtc gccgtggcgg cagaggcggc gcgcaacacc cgccacaact ccggccactg
      961 cccgtgaacg tcgccgatcg cgatgacctt gatcatcgac gcacccggcg agcgccacgc
     1021 ggccgggcgg tcatgccggc tgcgcttccc tctgcaggag gatggaccgc aactgtcggt
     1081 acagctcgcg cgactcctcc ggcgtccgcc cgtagccgcc gtagcgcacg acgagcttcg
     1141 cggcctccct gccgagaccc tcggcgttga ggcgctcgat gagccgctcg atgacctcgt
     1201 gaccgcccga ggcgctcccc gtcttctgcg gcggcggttc ggtcgctcgc tcggagtccg
     1261 cctcttcgac tgcggcgatg gccgcggcgg cggccgtggc ggcggccacg gcggccgtag
     1321 cggcaacgac tccttgacca gcctgcgcag tgagaggacc aggcccggcc gaagcgcaca
     1381 ccggctccgg ttcgaacagg gcctcgccgg cctcagcgtc gaagtcgacc cagtagctcg
     1441 ggtcgtccga cacgtacggc agtcgcaggc cgaactgcgc cgcgcagcgt gccagcgcga
     1501 actcgccgga gcgcgccaag gcgtcgggca gggggacacc acccgacggg acgcagagcg
     1561 cgatcacgtc cgagcgcgtc gcaccgcgaa cggtgaggtt ggcgaccagc gcggacgcgc
     1621 ctaagggggc aagggagaac gaccaaccca agcgaccgca ggcgacatcg agccgctctg
     1681 cgatggccgc gcgcgttgca taaggctcca gacgtgctct cgcggcaccg ggctccacct
     1741 ccgcgacgtg ccaccgcaag gcatcggacg ccagaggcgg agcgagtgtg gcactcagct
     1801 cgggctgcac ctcagtacta tagcaacgcc caaagcgcgc aggacgcgaa agaagccacc
     1861 ctcacgcgcc aaccgtcgga gggcgccgca cgagagtgcg gtcacacggg ggtgcggtaa
     1921 tgtgagccca tgtcgaaacc tcgcgccgta ccgaaccgcg ctggcaagtc gccgtcgccg
     1981 tctcccgcgg gaacagggcg cgtcggctcg ttcttggcga tcatcgccgc ctccgtcctg
     2041 accctcggcg ccgcgctcgc gcaaaccgcg cccgtcgtcg ggttggacgc gtacgacttc
     2101 gaggtgggtc agactctcaa cgtgagcggc gaccgcctca cacccggctc cgcctaccgc
     2161 atcgctgtcg ggccggtcgg gggggcggcc acggtcagcg tcgtgcaagc cgacgccaac
     2221 ggctcgatcc gctaccagac ggtgctcgag gagccgggcg accacggcgt gagcatcgac
     2281 ggcgatgaca tccacgcggt gttcacgatc agcgtccgcg ggccggacgc cgctccggaa
     2341 agcgcccagg aagcgggacc tgagcccgcc gaggaagcca gccagggcgg cggcgaggcg
     2401 ccggccacgc aagacgctca accagcgcaa gagacgccgg agacaccgag ccagcaaggc
     2461 gcgaccggcg cgaccgccgc gcccacgcag ccgaaccagg agccgcccgc cgcgaacgag
     2521 ccgggcggcc tggcccagcc gagccctgtc cagacctacc cgacggccag gctggtgggc
     2581 aacgaggtcc aaggtgcgca aggcgaggag atcgtgtggc gcctgagctt cccgagcggg
     2641 tccggggaga cggcgggcct cgtccagtcc gggctcgacg tgtacgtcgg ccacggcaac
     2701 cacgtcctga gggtcgacgc caggaccggc aagatcacga agcggcatcg cctaccggcc
     2761 caggtcaccg cgctacaggg gcgcggcggc gcgctgaacg caacggtcac ctactcgacc
     2821 ggtgccgaag gcagcttcgc gatcctcgag acgtcccaag acccgaacga gcctttcgac
     2881 gacgacccgg cgctctatgg ctggttgcgc aaggaggccg cgggcggcga cccgcaggag
     2941 cgcttgcaga acgacgagac gaacccttgg ctctacctcg aggccgcccg cgcggccacg
     3001 ccggg
//
LOCUS       DAOQYE010000143         2996 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_35148_length_2996_cov_4.048963, whole genome shotgun sequence.
ACCESSION   DAOQYE010000143 DAOQYE010000000
VERSION     DAOQYE010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2996
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35148_length_2996_cov_4.048963"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="PKN52_03370"
     CDS             <1..417
                     /locus_tag="PKN52_03370"
                     /inference="COORDINATES: protein motif:HMM:NF016975.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HNQ99013.1"
                     /translation="VVVTGGHAYDKEAFEALFRKLVTGLHSMGVVGSQDAPALARYIR
                     FLTRWLELEAFLAENGQTFVKRGRPTEAEPQGPVIDVRTWPHAKESRQLAGRLIEIER
                     EFGMTPAARARLAIEAGAGDTKGSDAGGGKARFLSG"
     gene            386..2278
                     /locus_tag="PKN52_03375"
     CDS             386..2278
                     /locus_tag="PKN52_03375"
                     /inference="COORDINATES: protein motif:HMM:NF015321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HNQ99014.1"
                     /translation="MAVRLDSSPADEAFGDVDRRLRAKVEAEAKADRWMAARRVLAKH
                     DLLGELPEDELRAKCADEEWVALCSAVALKPAEIILLLPLYDPWDQAEGFRFDEAKAV
                     LAVEFFHELLTLTEGEWAGRPFQLELWQQAILMNLFGWVRADGSGLRRFREAMIEVPR
                     KNGKSPFLAGIALLCLVADNEPGAQVYSAAGDRSQAAIIYEHARKMVENDEQLRRAVK
                     ARDSMKLLEYARLGSLYRALSKESKTKHGLNVHAAIVDELHVQVGGELVDTLTSATGT
                     RRQPLIIYITTRDFDREGSICNEKERHADRVRRGLVKDPAFLPALWLLEDYVDTRDAK
                     AWHDPLAHVLVNPNLGISIKGDEFAREFLLAQQNPRLRNSFIRLRLNGRTQSNVAWLP
                     MDLWDSCGTPRMEHPRLGFNGRDGREHFIKVLGLDGARCFGGLDLSSNRDLCSFVLYF
                     PEAVACLLYAWSPRFGAEQRESVEGISYLALADEGWLNLVDGNIQDYDEIRAMVNDLG
                     GRFRIESIGYDRFGGQKLVTELASDGFEIVKVGQGYYSMSSPSKALERMVLGGMLHHG
                     GDPLLRWAAGNVMVSEDPAGNIKPDKAKSSDRIDPITALVMAIGEEDEDEGDGDAYAE
                     RGFLTL"
     gene            2275..2466
                     /locus_tag="PKN52_03380"
     CDS             2275..2466
                     /locus_tag="PKN52_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99015.1"
                     /translation="MRSQRLKVLAALAGVLVRLALAIGAWGAIVYGVSRIHEPAAWIV
                     GGALLWLDLFISSRERPKP"
     gene            2463..>2996
                     /locus_tag="PKN52_03385"
     CDS             2463..>2996
                     /locus_tag="PKN52_03385"
                     /inference="COORDINATES: protein motif:HMM:NF016732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HNQ99016.1"
                     /translation="MSLLRWIAGARSETGARTADLLSDDKWLIDALGGRVSSSGKRVR
                     GQQALTLDGYYACVRNIAEDLGKMTREVRKRLEDGGSEKITKGNAVRLIRRRASPSIS
                     DQAFVEAMALSALHRGFGLAEIQRDRDSGEAIRMWPIHAGLVEIKEDAGGVYALVHGF
                     NGNPAVVIPWEDMFYIHG"
BASE COUNT          477 a         1003 c         1087 g          429 t
ORIGIN      
        1 gtggttgtca cgggggggca cgcgtacgac aaggaggcgt tcgaggcgct gttccgcaag
       61 ctcgtcaccg gcctgcactc catgggcgtc gtgggctcgc aggacgctcc ggcgctggcg
      121 cgctacatcc gcttcctgac gcggtggctc gagctcgagg ccttcctcgc cgagaacggg
      181 cagacgttcg tcaagcgtgg gcggcccacg gaggccgagc cccagggccc ggtcatcgac
      241 gtgcgcacct ggccgcatgc caaggagtcg cgccagctcg cggggcgcct gatcgagatc
      301 gagcgggagt tcggcatgac cccggcggct cgggctcgcc tggccatcga ggccggcgcc
      361 ggcgacacga aggggagcga cgccggtggc ggtaaggctc gattcctctc cggctgacga
      421 ggccttcggc gacgtcgatc gtcggctgcg cgccaaggtg gaggcggagg ccaaggccga
      481 ccgctggatg gctgcccgcc gcgtgctggc caagcacgac ctgctgggcg agctccccga
      541 ggacgagctg cgcgccaagt gcgcggacga ggagtgggtg gcgctgtgca gcgcggtcgc
      601 cctcaagccg gccgagatca tcctcctgct cccgctctac gacccgtggg accaggcgga
      661 gggcttccgc ttcgacgagg ccaaggccgt gctggccgtg gagttcttcc acgagctcct
      721 gacgctgacc gagggcgagt gggctgggcg ccccttccag ctcgagctct ggcagcaggc
      781 catcctcatg aacctgttcg gctgggtgcg cgccgacggc tcgggcctgc gccggttccg
      841 ggaggcgatg atcgaggtgc cccgcaagaa cgggaagagc cccttcctgg ccggcatcgc
      901 cctcctgtgc ctcgtggccg acaacgagcc tggggcccag gtctacagcg cggcgggcga
      961 caggtcgcag gcagccatca tctacgagca cgctcgcaag atggtcgaga acgacgagca
     1021 gctccggcgt gccgtgaagg cccgcgactc gatgaagctg ctcgagtacg cccggctcgg
     1081 gagcctgtac cgggcgctct ccaaggagag caagaccaag cacgggctca acgtgcacgc
     1141 ggccatcgtg gacgagctgc acgtccaggt cggcggcgag ctcgtggaca cgcttacgtc
     1201 ggcgacgggc acgcggcgcc agccgctgat catctacatc acgacgcgcg acttcgaccg
     1261 cgagggctcg atctgcaacg agaaggaacg ccacgccgac cgggtccgtc gcgggctcgt
     1321 gaaggacccg gccttcctcc cagccctgtg gctgctcgag gactatgtcg acacgcgcga
     1381 cgcgaaggcc tggcacgacc cgctggccca cgtcctcgtc aaccccaacc tgggcatctc
     1441 gatcaagggc gacgagttcg ctcgcgagtt cctgctggcc cagcagaacc cgcggctgcg
     1501 caacagcttc atccggctgc gcctgaacgg gcgcacgcag agcaacgtcg cctggctccc
     1561 gatggatctg tgggactcgt gcggcacgcc ccgcatggag cacccgcggc tcggcttcaa
     1621 cgggcgcgac ggccgcgagc acttcatcaa ggtgctgggg ctggacgggg cgaggtgctt
     1681 cggggggctc gacctcagca gcaaccgcga cctgtgcagc ttcgtcctct acttccccga
     1741 ggccgtagcc tgcctgctgt acgcgtggtc gccccggttc ggggccgagc agcgggagag
     1801 cgtggagggc atcagctacc tggccctcgc cgacgagggg tggctcaacc tcgtggacgg
     1861 caacatccag gattacgacg agatccgggc catggtgaac gacctgggcg gccgcttccg
     1921 catcgagtcg atcggctacg accgcttcgg cggtcagaag ctggtgaccg agctggccag
     1981 cgacggcttc gagatcgtga aggtggggca gggctactac tcgatgagca gccccagcaa
     2041 ggcgctcgag cgcatggtgc tcggcgggat gctgcaccac ggcggggacc cgctcctgcg
     2101 ctgggccgcc ggcaacgtca tggtcagcga ggacccggct gggaacatca agccggacaa
     2161 ggcgaagagc tccgaccgca tcgacccgat cacggcgctc gtgatggcca tcggcgagga
     2221 ggacgaggac gagggcgacg gcgacgcgta cgccgaacgg gggttcctga cgctatgaga
     2281 tctcagcgac tcaaggtgct ggcggccctc gccggcgtgc tcgtgcggct ggccctggcg
     2341 atcggcgcct ggggcgcgat cgtctacggc gtcagtcgga tccatgaacc ggcggcgtgg
     2401 atcgtgggcg gtgcgctact ctggctcgac ctgttcatca gctcgaggga gcggccgaag
     2461 ccatgagcct gttgcgctgg atcgctgggg cacgttcgga gacgggcgca cgcaccgcgg
     2521 acctgctgag cgacgacaag tggctgatcg acgccctggg cgggcgcgtg tcctcgagcg
     2581 ggaagcgggt ccggggtcag caggcgctga cgctggacgg ctactacgcg tgcgtccgca
     2641 acatcgccga ggacctcggg aagatgaccc gcgaggtgcg caagcgcctc gaggacggcg
     2701 gcagcgagaa gatcaccaag ggcaacgccg tgcggctcat ccggcgccgc gcctcgccct
     2761 cgatcagcga ccaggccttc gtcgaggcca tggcgctctc ggcgctgcac cgcggcttcg
     2821 gcctggcgga gatccagcgc gaccgcgaca gcggcgaggc cattcgcatg tggccgatcc
     2881 acgctgggct cgtggagatc aaggaggacg ccggcggggt gtacgccctc gtgcacggct
     2941 tcaacggcaa cccggccgtg gtgatcccct gggaggacat gttctacatc cacggc
//
LOCUS       DAOQYE010000144         2991 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_35223_length_2991_cov_3.789510, whole genome shotgun sequence.
ACCESSION   DAOQYE010000144 DAOQYE010000000
VERSION     DAOQYE010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2991
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35223_length_2991_cov_3.789510"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..723
                     /locus_tag="PKN52_03390"
     CDS             <1..723
                     /locus_tag="PKN52_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HNQ99017.1"
                     /translation="AANFTPAEEDRPALLTHSEVETVFHEFGHLLHHVMCRVEVRARA
                     SGSVPWDFVELPSQIMENWTWEKAAIDLFAGHFKTGEPLSTELFDRLERSRTFMGAFA
                     QMRQLEYGTVDLALHRDYDPTGAEDPLTFGERVMRGFHPRPDFARGERLARFTHIFAG
                     GYAAGYYSYKWSEVLDADAFSRFAEEGIFNGATGRRFAAAILERGDADDADQLFRDFM
                     GRDPELDALIRRNLGVGAVRAA"
     gene            complement(710..1903)
                     /locus_tag="PKN52_03395"
     CDS             complement(710..1903)
                     /locus_tag="PKN52_03395"
                     /inference="COORDINATES: protein motif:HMM:NF024136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3810 family protein"
                     /protein_id="HNQ99018.1"
                     /translation="MALLGPAIQYAPWPQGWLDAGFMGWWFPRLTHLTTLSSAVGWSV
                     TAVALAGWAAVLAVCAAWALLGRSRPEAPLGRSFSGAIFGRSPSSGHRWWTFWLTLAW
                     PAALLVVAFPLTFGLAYRTGDLTAAVAARLDGRATDPWSTDDGESLAERLIAILMATA
                     PDAPSGEHAADMPAALVADAARCVASTAETVRREFLPATLAGGTRAGGTPVGGAGRTV
                     PGVLVAVPSRVKTLPAGTLLRGGYAGIVSPWLLEPHVDGGLPATAGLAVALHELAHTA
                     GFAREAEAEAIGLLAGIRCDDPAVKYAAALRLAGSLLTTADPETARAYVARWPERARQ
                     DSRAADAAALRYGDPRLQRAADAVYDAYLRRQGGADGLGEYANGTALAIALAMALPTT
                     GFTPP"
     gene            complement(1978..>2991)
                     /locus_tag="PKN52_03400"
     CDS             complement(1978..>2991)
                     /locus_tag="PKN52_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99019.1"
                     /translation="LALSLAQGADLLLLDEPSNDLDLEAIEALEAQLGETLEAGGTAL
                     ILVTHDRRLAERLTDEAWALEGRRVVRYGDVGAYLRGQPTAETDEDDGTAAGGADEAP
                     EQPGLPHGQDDLAPLENERSSLLGRLADPLGLTEREAERVRRRLRQVEEELVALFEAG
                     LQTPAPRHRVREGGLTVFGDVVDGRLAAVLMDESATPEGDPALRSAEAAGAALAGWPL
                     APGDMGAMGVLAAAEARASDGVAHLKLIERTDACLLTEARNALLDAGVRLAFTLLGVE
                     AVQTQSALPVASRWLRSAGDGWHTTRLGAFLETEGWRRGRPRRRGGKRRRPSGGDRRG
                     GGE"
BASE COUNT          414 a         1125 c         1062 g          390 t
ORIGIN      
        1 gcggccaact tcacgcctgc cgaggaggac cgaccggcgc tcctcaccca ctcggaagtg
       61 gagacggtct tccacgagtt cggccacctg ctccaccatg tgatgtgccg ggtggaggta
      121 agggcgaggg ccagcggcag cgtgccatgg gacttcgtcg agctcccgag ccagatcatg
      181 gagaactgga cgtgggagaa ggccgccatc gacctcttcg ccgggcattt caagacgggc
      241 gagcccctga gcaccgagct cttcgacagg ctcgagcgca gccgcacgtt catgggcgcc
      301 ttcgcccaga tgcggcagct cgagtacggc acggtcgatc tggctctgca ccgcgactac
      361 gacccgaccg gcgcggagga cccgctcacc ttcggcgagc gcgtcatgcg aggcttccac
      421 ccgcgcccgg acttcgcgcg cggcgagcgg ctggcgcgct tcacgcacat cttcgcgggc
      481 ggctatgcgg ccggttacta ctcgtacaag tggagcgagg tcctcgacgc cgacgccttc
      541 agccgcttcg cggaggaagg gatcttcaac ggcgcgaccg gacggcggtt cgccgccgcc
      601 atactcgagc gcggcgacgc ggacgacgcg gaccagctct tccgcgactt catgggccgt
      661 gaccccgagt tggacgccct gatccgccgc aacctcggcg taggcgccgt cagggcggcg
      721 tgaagcctgt cgtcggcagg gccatcgcca gcgcgatcgc caacgccgtg ccgttcgcgt
      781 actcgccgag accgtccgcg ccgccctggc gccgcaagta ggcgtcgtag acggcgtcgg
      841 cggcgcgttg caaccgcggg tcgccgtagc gcagtgcggc ggcgtcggcc gcccgcgagt
      901 cctggcgggc ccgctcgggc caccgcgcca cgtacgcgcg cgccgtttcc ggatcggccg
      961 tggtcagcag cgaacccgcc aaccggaggg cggccgcgta cttcacggcg gggtcgtcgc
     1021 accggatgcc cgccagcagc ccgatggcct cggcctcggc ctccctggcg aagcccgccg
     1081 tgtgcgccag ctcgtgtagg gcgacggcca ggccggccgt cgccggcagt ccgccgtcga
     1141 cgtgaggctc gagcagccac ggcgagacga tcccggcgta cccgccgcgc agcagcgtgc
     1201 cggcaggcag ggtcttgacg cgtgagggaa ctgcgaccaa gacgccgggc accgtccgtc
     1261 ccgcaccgcc cacgggcgta ccgccggccc gcgtgcctcc cgcgagggtc gcgggcagga
     1321 actcccgccg cacggtctcg gccgtgctcg cgacgcaccg cgcggcgtcg gcgacgagcg
     1381 cggccggcat atccgccgca tgctcgcccg acggcgcgtc tggcgcggtc gccatcagga
     1441 tcgcgatcag ccgttccgct aggctctcgc cgtcgtcggt cgaccacggg tcggtcgccc
     1501 gaccgtccag acgcgccgcg acggcggccg tcaggtcgcc ggtgcggtag gcgaggccga
     1561 acgtcaacgg gaacgcgacc accagcaggg cggcgggcca cgcgagcgtc agccagaacg
     1621 tccaccagcg gtgtccactc gaagggcttc gaccgaagat ggcaccggag aagcttcgac
     1681 cgaggggggc ttccggacgg cttcgaccga ggagggccca ggctgcgcag acggccagga
     1741 ctgcggccca cccggcgagc gcgacggcgg taacgctcca gccgacggcg ctcgacagcg
     1801 tcgtcaggtg cgttagtcgc gggaaccacc aacccatgaa gccggcgtcg agccagcctt
     1861 ggggccaggg ggcgtactgg atggccggac cgaggagcgc cacgatggcg gctgcaacga
     1921 gcgctcgtgt gggtgcagac cggctggcgt cagacggcct tgccgtgcgt ggccggctca
     1981 ctcgccgcct ccgcgccggt cgcccccgct gggtcgccgc cgtttgcccc cccgccggcg
     2041 cggccgcccc cgtcgccacc cctcggtctc gaggaaggcg cccagtcttg tcgtgtgcca
     2101 gccgtcgccc gctgaccgca gccagcgcga tgctaccggg agggcggact gcgtctggac
     2161 ggcctcgacg cccaggagcg tgaaggcgag gcgcacgccg gcatcgagaa gggcgttgcg
     2221 cgcttcggtc aggaggcagg cgtccgtccg ctcgatgagc ttcagatgcg ccaccccgtc
     2281 tgacgcgcgc gcctcggccg ctgcgagcac gcccatggcc cccatgtcgc caggcgcgag
     2341 cggccaccct gccagcgccg cccctgcggc ctcggcggac cgtagagcgg gatcgccttc
     2401 gggcgtcgcg ctctcgtcca tgagtacggc cgcgagcctg ccgtccacca cgtcgccgaa
     2461 caccgtcagc ccgccctcgc gcacccggtg gcgtggcgcg ggcgtctgca acccggcctc
     2521 gaagagcgcc acgagctcct cctctacctg acgcagccgg cgcctgacgc gctcggcctc
     2581 gcgctcagtg agtccgagcg gatcggccag acgccccagc agggaggagc gctcgttctc
     2641 caacggggca aggtcgtcct gaccgtgggg gagcccaggc tgctcgggcg cctcgtcagc
     2701 cccgccggca gccgtcccat cgtcctcgtc cgtctccgcg gtcggctggc cgcgcaggta
     2761 agcgcccacg tccccgtagc gcacgacccg ccggccctcg agcgcccaag cctcgtcggt
     2821 cagccgctcc gccagcctgc ggtcgtgcgt gaccaggatc agggctgtcc caccggcctc
     2881 cagcgtctcg ccgagctgcg cctcgagcgc ctcgatggct tcaaggtcga gatcgttgct
     2941 gggctcgtcc agtagtaaca ggtcggcgcc ttgggccagg gagagggcga g
//
LOCUS       DAOQYE010000145         2983 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_35355_length_2983_cov_3.782787, whole genome shotgun sequence.
ACCESSION   DAOQYE010000145 DAOQYE010000000
VERSION     DAOQYE010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2983
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35355_length_2983_cov_3.782787"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..775)
                     /locus_tag="PKN52_03405"
     CDS             complement(<1..775)
                     /locus_tag="PKN52_03405"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ99020.1"
                     /translation="MTQKIVALQATKRYPSKAGMVTALEGFDLEVAEGEFVCLVGPSG
                     CGKSTFLRILAGLDDLTGGEARIVPGDDPRRPLNNVVFQEYAVFPWKTVRDNVAFGLQ
                     MRGIGKAERYAVTERWLTKVGLKRFADYYPSQISGGMKQRVSIARALANDPEVLLMDE
                     PLGALDAQTRAVLQEELLRIWEETHKTVLYVTHSIEEAVLLGDRVVLMTAHPGTKKSE
                     FVVDLPRPRDLATTATPEFAALTGAIWEDLRDEVRRAMEA"
     gene            complement(815..1855)
                     /locus_tag="PKN52_03410"
     CDS             complement(815..1855)
                     /locus_tag="PKN52_03410"
                     /inference="COORDINATES: protein motif:HMM:NF024772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNQ99021.1"
                     /translation="MIARTRKLLAALLVAALVLGAASAQDTHGVAPLEPVQKVRVAYV
                     PILKFATLYVAADRGLFAQYGLDVDLESVASGTEAIAFLEQGQIDVGGIAIVTSLWNG
                     WNQGIDIRVFAPGGLEPFVDSPTKVLVRKDLYDSGAVTNAGDLRGRTVAFAGGPGSGG
                     EYLTAKALEAYGLTIRDVEIVSMGNGDIPAAFENGSIDAALLGSPYADQVEEAQTAVA
                     IATDLVPGLMTVAFVGSGKFLNERPEVAERFALALLDAARLMQGDDYYSPENIAAYLH
                     YVNSTEDAIVNGTPVLYDPNLTIPVEGLADVERVHRENGRTDYETPIDLNVVISTVFA
                     ERALAAAGTYQP"
     gene            2177..2881
                     /locus_tag="PKN52_03415"
     CDS             2177..2881
                     /locus_tag="PKN52_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HNQ99022.1"
                     /translation="MTPGIGVAFAAGVLSFLSPCVLPLVPSYLAYVGGSAEAKRAVLL
                     RNALAFVLGFSLVFIALGAGASALGSLLRANQRALTLGGGVLVILFGLVMLGVIKLPW
                     LYRDTRMQYSGETRTPFGAVLLGMAFAAGWTPCIGPILGAILTMASAAGTLSAGVGLL
                     AVYALGLAVPFLAAALLLDPFMRFSRRFRRFLPWVERVAGALLLVAGVLMVTGLYTVL
                     NGYLINITPAWLLDNL"
BASE COUNT          428 a         1017 c         1047 g          491 t
ORIGIN      
        1 gggcctccat ggcgcgcctg acctcgtccc tgaggtcctc ccagatggcg cccgtcaacg
       61 ccgcgaactc cggcgtggcg gtcgtggcga ggtcgcgcgg acgcggtagg tcgaccacga
      121 actccgactt cttcgtgccg gggtgggccg tcatgagcac gaccctgtcg ccgagcagga
      181 cggcctcctc gatggagtgc gtcacgtaca gcacggtctt gtgggtctcc tcccagatgc
      241 ggaggagctc ctcttgcagc accgcgcgcg tctgggcgtc gagcgcgccg aggggctcgt
      301 ccatcaggag gacttccggg tcgttggcca aagcgcgcgc gatggagacg cgctgcttca
      361 tgcctccgga gatctgcgac gggtagtagt cggcgaagcg cttcaggccg accttggtga
      421 gccaccgttc ggtcaccgcg taacgctctg ccttgccgat cccgcgcatc tgcaggccga
      481 aggccacgtt gtcgcggacc gtcttccacg ggaagaccgc gtactcctgg aagacgacgt
      541 tgttgagcgg ccgacggggg tcgtcgccag gcacgatgcg ggcctcgccc cccgtgaggt
      601 cgtccaggcc cgccaggatg cgtaggaacg tcgacttgcc gcacccggat ggcccgacca
      661 ggcagacgaa ctcgccctcg gcgacttcga ggtcgaaccc ctcgagggcg gtgaccatgc
      721 ccgccttcga ggggtacctc ttcgtcgctt ggagcgcgac gatcttctgc gtcatgcctg
      781 ccggcctcgg cgtgcgttca ggagcgggcc ggacctaggg ctggtaggtg ccggcggcgg
      841 ccagggcgcg ctcggcgaac accgtcgaga tgacgacgtt caggtcgatg ggcgtctcgt
      901 agtcggtacg accgttctcg cggtgcacgc gctcgacgtc ggcgaggccc tcgaccggga
      961 tggtcaggtt cgggtcgtag aggacggggg tgccgttgac gatcgcgtcc tcggtcgagt
     1021 tcacatagtg caggtaggcg gcgatgttct ccggcgagta gtagtcgtcg ccctgcatga
     1081 ggcgcgcggc gtccaggagt gcgagcgcga agcgttcggc gacctcgggg cgctcgttga
     1141 ggaacttgcc ggagccgacg aacgccacgg tcatgaggcc aggcacgagg tcggtcgcga
     1201 tggcgacggc cgtctgggcc tcctccacct gatcggcata cggggagccg aggagggccg
     1261 catcgataga gccgttctcg aacgcggccg ggatgtcgcc gttgcccatc gacacgatct
     1321 cgacgtcgcg gatggtgagg ccgtatgcct cgagcgcctt ggccgtgagg tactcgccgc
     1381 cgctgccggg gccgcccgcg aacgccacgg tgcgccctct gaggtcgccc gcgttcgtca
     1441 cggcgccgga atcgtagagg tccttacgga cgaggacctt ggtcgggctg tccacgaacg
     1501 gctcgaggcc gccgggggcg aagacgcgga tgtcaatccc ctggttccag ccgttccaga
     1561 gggacgtgac gatcgcgatg ccgccgacgt cgatctgccc ttgttcaagg aaagcgatgg
     1621 cctcggtgcc ggacgccacg gactcgagat cgacatcgag gccgtactgg gcgaacagac
     1681 cacggtcggc ggccacgtag agcgtcgcga acttgaggat gggcacgtac gcgacgcgca
     1741 ctttctggac gggctcgagc ggcgcgacac cgtgcgtgtc ctgcgcggac gcggcgccga
     1801 ggaccagggc cgcgaccagc agcgcggcaa ggagctttct cgtacgagcg atcatggggc
     1861 ctccacgtgt cggacggccg cagtgcgacg ggtccgtgag aatgtcaggg ggtcatgtta
     1921 cccctcgcgg agcgcaaggg gatcccaacc gaagtcgagg gtcacgcgcc gccgactgga
     1981 gctggtcatg cgcgggacaa cgcgagggaa cgaagcgcga gggagcaatg tcctcccccg
     2041 accacgaacg gttctcgtat catgttcagg ccgccatgaa ccctcacgcc ccctcacgaa
     2101 ccgcccaccc gtcgtaccta cgagtgcgag cggcaaagag cgttcgcgct cggcggcgcg
     2161 cgaacggcgt ggcgccatga cgccaggcat cggcgtcgcg ttcgcggccg gcgtgctctc
     2221 cttcctctcg ccatgcgtgt tgccgctcgt gccctcctac ctcgcgtacg tcggcggcag
     2281 cgccgaggcc aaacgggccg tgctccttcg caacgccctc gcgttcgtgc tcggcttctc
     2341 gctcgtcttc atcgcgctcg gggcgggcgc cagcgcgctc ggctccctcc tgcgggccaa
     2401 ccagcgggcc ctgaccttgg ggggcggcgt gctcgtgatc ctcttcgggc tcgtgatgct
     2461 gggggtcatc aagctgccgt ggctgtaccg cgacacgcgc atgcagtact cgggcgagac
     2521 gcggaccccg ttcggagccg tgctcctcgg catggcgttc gcggcaggtt ggacgccctg
     2581 catcgggccg atcctcggcg ccatcctcac gatggcgagc gcggcgggca cgctgagcgc
     2641 cggcgtcggc ctgctcgccg tctacgcgct cggactggcc gtccccttcc tcgccgcggc
     2701 cctcctcctc gaccccttca tgcgcttctc gcgccggttc cgccggttcc tgccgtgggt
     2761 cgagcgggtg gcgggcgcgc tgctcctcgt ggccggcgtc ctcatggtca ccggcctgta
     2821 caccgtgctg aacggctatc tcatcaacat cacgcccgcc tggctcctcg acaacctatg
     2881 aggcccgggc cgccatgagc acgggcgcgg acgagctcgc catcgagctc aacgaggtga
     2941 cacggcgcat gggcggcgag tacatcctcc ggggcatcaa cct
//
LOCUS       DAOQYE010000146         2973 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_35523_length_2973_cov_3.111035, whole genome shotgun sequence.
ACCESSION   DAOQYE010000146 DAOQYE010000000
VERSION     DAOQYE010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2973
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35523_length_2973_cov_3.111035"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..641
                     /locus_tag="PKN52_03420"
     CDS             <1..641
                     /locus_tag="PKN52_03420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99023.1"
                     /translation="GDKLTDEYGNPDPLTYASRGTSGNGWDYRALTMSLTKGDGSVYD
                     VLSLVARFGNQAVPVIAVEPMSTLRCVSYDGQFGLDVANVLYSLSLGTPTYDHALESD
                     LVGEWFSSDGSAGNLYVFGANGHYIDASAVGGVVEVTPGEWRDRYASWSGTGRWAAVG
                     DVLGFFPSGGQATSHFALRFEFRIYNGDWREAACWTDEYEGNPYMYCTRRQE"
     gene            688..2097
                     /locus_tag="PKN52_03425"
     CDS             688..2097
                     /locus_tag="PKN52_03425"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HNQ99024.1"
                     /translation="MLDHDTYASLIQQGRLAGADFVEVYVERWRRRHLRTIDGQVEDA
                     TSALEHGAGIRLFFGTDVVYGYTNDLTDAALAELLSSLVTVRGGAPGAPGTPDAKGRG
                     GLDLRRLDVGPDIHAPSVPFDARDKAWRLERLRELDAGARIAPEIRQVEARLLEWEQD
                     VSVATSDGAVVDDRRVRTRLIAQAIASDGKDTQTAHHGPGLSMGLELLDTHPPAEIGR
                     RAAEVALLNLRARKAPAGPMPVVIGNAFGGVIFHEALGHLLETTSVARNASALTGKLG
                     EQVASEVVTYVDDGTTPNGWGSSRFDDEGLPTERTVLIRDGVLESYMVDRWGSRMTGY
                     RATGSGRRQDYTFAPTSRMRNTFVLPGTTPKDDLFEGITLGLYAKSMGGGQVKPGSGE
                     YNFAVNEGYMIRNGELAEPVRGAMLVGKGPESIKRIVAVSDDLSTAPGMCGSMSGSLP
                     VEVGQPHLLVSEIIVGGEA"
     gene            complement(2180..>2973)
                     /locus_tag="PKN52_03430"
     CDS             complement(2180..>2973)
                     /locus_tag="PKN52_03430"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNQ99025.1"
                     /translation="GGLPESEVDAALDGVLGVARGPASVQSGQSVEGRPQGVKQGQGI
                     LWAHGARGVLVLSNALADAALAKLAVAGLPVTLVSHYSAAPGQPTVMFDNRQGVKQLM
                     RHVVADCGRRRPVYIGGDSSQLDGREREAAFRDELLRHDLRVPEGHMLVGGFTPGLAR
                     QALEGFTASGSDFDAVVAADYLMAIAAAETLREAGVRIPEDVVVVGYGDGPEAEAAGV
                     TTVAADVVELGRRAARQLVAQLSPERPISGRTLLSTHLVRRASSC"
BASE COUNT          474 a         1064 c         1012 g          423 t
ORIGIN      
        1 cgggcgacaa actgactgac gagtacggca atcccgaccc gttgacgtac gcctcgcgcg
       61 gcaccagcgg gaacggctgg gactaccgcg ccttgacgat gagtctcacc aagggtgacg
      121 gttctgttta cgacgtcctg tcgttggtgg cgcgcttcgg gaaccaggct gtacccgtta
      181 tcgcggtgga gccgatgagc accctgcgct gcgtgagcta cgacggccag ttcgggctgg
      241 acgttgccaa cgtcctatac tccctcagcc tcggcacccc gacgtacgac cacgccctcg
      301 aatccgacct cgtcggagag tggttctcta gcgacggcag cgcagggaac ctgtacgtgt
      361 tcggagccaa cggacactac atagacgcct ccgccgtagg gggcgtggtc gaggtcacgc
      421 cgggcgagtg gcgagaccgc tacgcctcct ggtccgggac ggggcgatgg gcggccgtgg
      481 gcgacgtgct ggggttcttc ccctcgggcg gacaagcgac gagccacttc gcgctccggt
      541 tcgagttcag gatctacaac ggggactggc gtgaagcagc gtgctggacg gacgaatacg
      601 aaggcaaccc ttacatgtat tgcacacggc gccaggagtg agccgcttgg cgacggcggt
      661 ccggcagtgg cgcggtacca tccgatgatg ctcgatcatg atacgtacgc ttccctcatc
      721 cagcaggggc ggctagccgg cgcggacttc gtcgaggtct acgtcgagcg gtggcgccgc
      781 cggcacctgc ggaccatcga cggccaggtc gaggacgcga caagcgccct cgagcacggc
      841 gccggtatcc gactgttctt cggcaccgac gtcgtctacg gctacacgaa cgacctcacg
      901 gacgccgcgc tggccgagct gctaagcagc ctggtgaccg tgcggggcgg cgcgcccggc
      961 gcgcccggga cgcccgatgc caagggccgc ggcggcctag acctgcgccg actggacgtc
     1021 ggccccgaca tccacgcgcc gagcgtgccg ttcgacgcgc gcgacaaggc ctggcgcctc
     1081 gagcgcctgc gcgagctcga cgccggcgcg cgcatcgctc cggagatcag gcaggtggaa
     1141 gcgcggctcc tcgaatggga gcaggacgtg agcgtcgcca cctccgacgg cgccgtggtg
     1201 gacgacagac gcgtccgcac gcgcctgatc gcgcaggcca tcgcctccga cggcaaggac
     1261 acccagacgg cccaccacgg gccgggcctg tcgatgggcc tcgaactcct cgacacccat
     1321 ccgcccgcgg agatcggcag acgcgcggcc gaggtcgccc tcctgaacct gcgcgcgcgc
     1381 aaggcgccgg ccgggcccat gcccgtcgtc atcggcaacg ccttcggcgg ggtcatcttc
     1441 cacgaggccc tcggtcacct actcgagacg acgtcggtcg cccgcaacgc gtccgcgctc
     1501 acgggcaagc tcggcgagca ggtcgcgtcg gaggtcgtca cctacgtcga cgacggcacc
     1561 acgccgaacg gctggggctc ctcgcgcttc gacgacgagg gcttgccgac cgagcgcacc
     1621 gtgctgatcc gcgacggcgt cctcgagagc tacatggtcg accggtgggg ctcgcggatg
     1681 accggctacc gcgccaccgg ctcgggacga cgccaggact acacgttcgc gccgacgtcg
     1741 cgcatgcgca acacgttcgt cctccccggc accacgccga aggacgacct gttcgagggc
     1801 ataacgctcg gtctctacgc caagtcgatg ggcggcggtc aggtcaagcc cggctcgggc
     1861 gagtacaact tcgccgtcaa cgagggctac atgatccgca acggcgagtt ggccgagccc
     1921 gtgcgcggcg ccatgctcgt cggcaagggc cccgagtcca tcaagcgcat cgtggccgtc
     1981 tccgacgacc tctccaccgc gcctggaatg tgcggctcca tgtctgggtc gctgccggtc
     2041 gaggtggggc aaccgcacct gctcgtcagc gagatcatcg tgggcggcga ggcctgaggg
     2101 tcttcagcgc ggcgcaggca gtgcgagcgg tccgacacat gacctcgcgg acccggaggc
     2161 ccgggcgccg ggcccgctct caacaactgc tggcgcgcct caccaagtga gtgctaagga
     2221 gcgtccggcc gcttatgggc cgctcgggcg agagttgcgc caccagttgc ctggcggcgc
     2281 gcctacctag ttcgacgacg tcggcggcca cagtggtcac gccggccgcc tcggcttccg
     2341 gcccgtcgcc gtagccgacg accaccacgt cttccgggat gcgcacgccg gcctcgcgaa
     2401 gcgtctcggc cgcggcgatg gccatgaggt aatcggcggc caccacggcg tcgaaatcgc
     2461 tcccggaagc cgtgaaccct tcgagcgcct ggcgtgccag ccccggcgtg aagcccccga
     2521 cgagcatgtg accctcgggc acgcgcaggt cgtgccgcag gagttcgtcg cggaaagccg
     2581 cctcgcgttc ccgaccatcg agctgcgagg agtcgccgcc gatgtacacg ggccgacgcc
     2641 tgccgcagtc ggcgaccacg tgccgcatca gctgcttgac gccctggcgg ttgtcgaaca
     2701 tcaccgtcgg ctggccaggg gcggccgagt agtggctcac gagcgtcaca ggtaggccgg
     2761 ccaccgccag cttggcgagg gcagcgtcgg cgagggcgtt ggacaggacg agcaccccac
     2821 gggcaccatg cgcccacagg atgccctgcc cctgtttgac cccttggggc cgcccttcga
     2881 cactctgccc cgactgaaca ctcgccggtc ctcgtgcgac acccaagacg ccatccaacg
     2941 cggcgtccac ctcggactcc ggcagcccgc cga
//
LOCUS       DAOQYE010000147         2970 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_35585_length_2970_cov_2.836364, whole genome shotgun sequence.
ACCESSION   DAOQYE010000147 DAOQYE010000000
VERSION     DAOQYE010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2970
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35585_length_2970_cov_2.836364"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="PKN52_03435"
     CDS             <1..298
                     /locus_tag="PKN52_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99026.1"
                     /translation="VAVHQLSGDLVARVRRSLTWLQQGEPVESLDVALRSGCQGGSCT
                     GRCSKGARSPEAAPEALIFTAHREMRTVDGILLPQVARVVVDPRGRIVKMAVTR"
     gene            336..1163
                     /locus_tag="PKN52_03440"
     CDS             336..1163
                     /locus_tag="PKN52_03440"
                     /inference="COORDINATES: protein motif:HMM:NF016274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HNQ99027.1"
                     /translation="MPDLTQSQVRTLEDEAALLKKSLQALIPQVESGRVALTDWGFNA
                     WGKKYAPWSDDDRAPATLAARLGMRRFVAPAVMEGGSLELNGHGVCLTTRSCLLEPNR
                     NPDLGPAELELLLRDYLGVTQLVWLEEGLEGDHTDGHIDTIVRFTDDDTIVCAVEPRE
                     DDPNHATMARNLEQLQSLRTAKGAPYRVVELPLPLKHMELEGQRLPPTYANFYVGNGF
                     VIVPQYDDANDEQALRILRPLFPGREVIGLGAVSLITGGGAFHCVTQQQPQGEVERA"
     gene            1156..2037
                     /gene="aguB"
                     /locus_tag="PKN52_03445"
     CDS             1156..2037
                     /gene="aguB"
                     /locus_tag="PKN52_03445"
                     /EC_number="3.5.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0050126 - N-carbamoylputrescine amidase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoylputrescine amidase"
                     /protein_id="HNQ99028.1"
                     /translation="MPEPKKSPELVRLAVVQMSCSHVLEENLAKAEAFVRGAAALGAN
                     VILLQELFENLYFPQLEREELFALAHPVKGHPFIERFARLAGELGVVLPVSFFEQAGQ
                     AYFNSLAMVDSNGEVLGHYRKSHIPDGPGYEEKYYFNPGDSGFKTWRTRFGNIGVGIC
                     WDQWFPEAARIMALKGADLLLYPTAIGSEPDEAGGLDTRDLWRRAMLGHAVSNVAYVA
                     AANRVGREGSATFYGSSFISDYEGELLADADRTSEVILTADLDLAAARTRRAGWGFFR
                     DRRPELYRALLTLDGEE"
     gene            complement(2074..>2970)
                     /locus_tag="PKN52_03450"
     CDS             complement(2074..>2970)
                     /locus_tag="PKN52_03450"
                     /inference="COORDINATES: protein motif:HMM:NF017885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HNQ99029.1"
                     /translation="ENVTGVPARIWTSLEEEYRLALARRDENERRREDAKLVDSDLYR
                     AMVKLGCVEGTRDPLAKARALCKFFGTDSLSYVKDAKHVGYAFRLGDRDGASGFALAA
                     WLRMAELEAKEMSPDPFDATGLQSVIEGLRSLTFELPSRWLPEARERLASVGVALAVI
                     PHLPKTFVNGATFWPSPHKAVVALSLRGSWADIFWFTLFHELGHVLLHGKRDPHVSLR
                     DTQEHEVEADRFAVDRLIPRRAYEEFVFKQEFSTQAVEEFSKTVGIAPGVVVGRLQHD
                     KLVPHNRLNELRERYRFEARAV"
BASE COUNT          514 a         1003 c          959 g          494 t
ORIGIN      
        1 ggtggcggtc caccagctct ccggcgacct cgtcgcccgc gtccggcgca gcctcacctg
       61 gctgcagcag ggtgaaccgg tcgagtcgct ggatgtggcg ctgcgttcgg gctgccaggg
      121 cggctcgtgc accggccgct gtagcaaggg ggctaggtcg ccggaagcgg cgccggaagc
      181 cctgatcttt accgcccacc gcgagatgcg caccgtggat ggcatcctgc tgccgcaggt
      241 ggcccgggtg gtcgtggacc cgcgggggcg gatcgtgaaa atggccgtga cccggtaggg
      301 agcagaccgg cagggttgac aaaaggtggt ggacgatgcc cgatctaacc cagtcccagg
      361 tgcgtaccct ggaggatgaa gccgcgctgc tgaaaaagtc actgcaggcg ctgatcccgc
      421 aggtcgaatc cggccgcgtg gccctcaccg actggggctt caacgcctgg ggcaagaagt
      481 acgccccgtg gtctgacgac gaccgcgcac cggccacgct cgcagcgcgt ctcggcatgc
      541 ggcgcttcgt cgcccccgcc gtcatggagg gtggctcgct cgagctgaac ggccacggcg
      601 tatgcctcac cacgcgctcg tgcctccttg agccgaaccg caaccccgac ctcggcccgg
      661 ccgagctcga gctgctgctg cgcgactacc tcggcgtcac gcagctcgtg tggctggagg
      721 aaggcctgga aggcgaccat actgacggcc acatcgacac tatcgtgcgc ttcacggacg
      781 acgacacgat cgtgtgcgcc gtcgaaccgc gcgaggacga cccaaaccac gccaccatgg
      841 cgcgcaacct ggagcagctc cagagcctgc gtacggccaa gggtgcgccg taccgggtcg
      901 tcgaactccc cctccccctc aagcacatgg agctggaggg ccagcgcctg ccgcccacct
      961 acgccaactt ctacgtaggt aacggcttcg tgatcgtgcc gcagtacgac gacgcgaacg
     1021 acgagcaggc cttgcgtatc ctgcgcccgc tcttccccgg ccgcgaggtc atcggcctcg
     1081 gcgccgtgag cctcatcacg ggcggcggcg ccttccactg cgttactcag cagcagcccc
     1141 aaggagaggt cgaacgtgcc tgagcccaag aagagccccg agctggtccg cctcgcggtc
     1201 gtccagatga gctgctcgca cgtgctggag gagaacttgg cgaaggctga ggcattcgtg
     1261 cgcggggcgg ccgccctagg tgccaacgtc atcctcctgc aggagctctt cgagaacctc
     1321 tacttcccgc agctcgagcg cgaggaactc ttcgcgctgg cgcaccccgt gaaggggcac
     1381 cctttcatcg agcgcttcgc caggctcgcc ggggagctgg gtgtggtgct gcccgtctcg
     1441 tttttcgagc aggccgggca ggcgtacttc aacagcctgg cgatggtgga ctcgaacggc
     1501 gaggtcctgg gccactaccg caagagccac atcccggacg gccccggcta cgaggagaag
     1561 tactacttca atccgggcga cagcggcttc aagacgtggc gcacgcgctt cggcaacatc
     1621 ggcgtcggca tctgctggga ccagtggttc ccggaggccg cgcgcatcat ggcgctgaag
     1681 ggcgccgacc tgctcctcta cccgaccgcc ataggcagcg aacccgatga ggcgggcggg
     1741 ctcgacacgc gcgacctgtg gcgccgcgcc atgctcggcc acgccgtcag caacgtcgcg
     1801 tacgtggccg cggccaaccg cgtggggcgc gaggggagcg cgaccttcta cggctcgtcg
     1861 ttcatctcgg attatgaggg cgagctgctc gccgacgccg accggacctc ggaggtcatc
     1921 ctaaccgccg acctcgacct ggcggcggcg cgcacgcggc gggcgggctg gggcttcttc
     1981 cgcgaccggc ggccggagct atatcgggcg ttgcttacgc tcgacgggga ggagtagctg
     2041 cggatcaggg ctaggagtag cttaggcttc cgttcaaacg gcccgcgcct cgaaccggta
     2101 gcgttctctc agttcgttca ggcgattgtg gggcaccaac ttatcgtgtt gtagacggcc
     2161 gaccacgact ccgggtgcga taccgaccgt cttactgaac tcctccactg cttgggtgga
     2221 gaactcctgc ttgaaaacga actcttcgta agccctgcgg ggaatcaacc gatctaccgc
     2281 gaaccggtcc gcttccactt cgtgctcctg cgtgtctcta agtgatacgt gcggatcgcg
     2341 cttcccatgc agcaagacgt gcccgagttc gtgaaaaagc gtgaaccaga agatgtctgc
     2401 ccaagagccg cgcaggctca acgcgacaac ggccttgtgc ggggatggcc agaatgtcgc
     2461 gccattgacg aaggtcttgg ggaggtgcgg gatcacggcc agtgcgacac cgacgctagc
     2521 caatctctca cgagcctcgg gcaaccagcg gcttggcagt tcgaacgtaa ggctccgaag
     2581 gccttcgatc acggactgca aaccagtagc atcgaacggg tccgggctca tctccttagc
     2641 ctcgagttca gccatgcgca accatgccgc cagtgcgaag cccgaagccc cgtccctatc
     2701 tccgaggcgg aatgcatagc caacgtgctt agcgtccttg acgtagctca gtgaatcggt
     2761 gccgaagaac ttgcagagcg cccgcgcctt ggctagcggg tcccgcgtgc cctcgacgca
     2821 acccagcttg accatggctc gatagagatc cgaatctaca agtttggcat cctcccggcg
     2881 acgctcgttc tcgtccctgc gcgcaagtgc cagccggtat tcctcttcca ggctcgtcca
     2941 gatcctcgcc gggacaccgg taacgttctc
//
LOCUS       DAOQYE010000148         2950 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_35911_length_2950_cov_3.011054, whole genome shotgun sequence.
ACCESSION   DAOQYE010000148 DAOQYE010000000
VERSION     DAOQYE010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2950
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35911_length_2950_cov_3.011054"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..1360
                     /gene="thrC"
                     /locus_tag="PKN52_03455"
     CDS             2..1360
                     /gene="thrC"
                     /locus_tag="PKN52_03455"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HNQ99030.1"
                     /translation="MIGYRSTRARRSGAPVVSFDEAMLAGLAPDGGLYWPTRIAPLPL
                     GWETASSPAELALAVLPPYVGDEAAAAIAGGLDFAWPVSRLSDDVFLLELFRGPTAAF
                     KDVGARSLARAMDAALADRGERVTVLTATSGDTGSAVADAFAGLERVRVAVLYPAGGV
                     SAVQEAQLTAARPGVRTFAVAGTFDDCQRLVKAAFADEGLRGTRLSSANSINVGRLLP
                     QLLYYLWGTARTWEASGRRQPLEIVVPSGNLGNLTAGMLAAEAGMPVAGFLAAHNRND
                     FFPRHLAGEADAYAFSGSVSTPSNAMDVGAPSNFERLYEGFGDDLRSRVAAVRVDDAA
                     TFERMRLTYERHGALVCPHTAVGLEALELHRAAARSLTGSRAGAGDASAGAHERGRST
                     PALVLATAHPAKFPESVLKATGVTPPVPATLERFERMPKRVEGLAAEPAALGAALLDW
                     TE"
     gene            complement(1415..2287)
                     /locus_tag="PKN52_03460"
     CDS             complement(1415..2287)
                     /locus_tag="PKN52_03460"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNQ99031.1"
                     /translation="MTRPVERLSEHVWRVELPCDTLPPYDHVNAYIVAKGGVGVVIDP
                     GSDRPEADALLASALAAADVRLVKGVLLTHTHPDHLGGVPSLLRSEASRTGSSPPVYA
                     HAAEAAELPGEWQATFLADGRVLTVGDVDVRAMHTPGHSHGHLSFAVTKAGNASPEAI
                     VVGDLASASGSVWVGLPEGDVGDYLTSLERLAALGAPVLGAGHGPAITAPTARLRELA
                     EHRLAREAQILAALELGPADAMEITRRVYPDHPESVLAFAEKSVLAHVAKLMRELRVV
                     HLGSDERGPFAVRR"
     gene            complement(2284..>2950)
                     /locus_tag="PKN52_03465"
     CDS             complement(2284..>2950)
                     /locus_tag="PKN52_03465"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNQ99032.1"
                     /translation="GAPGLTYAPGAPALLGQRLPSDLRAAGHEANVTYGLTPEGVGYV
                     RLPDLATTGTAAAVRNAVKNVQAEGASAIVLDLRGNPGGRLVTMMQVAGVFTSGLLWR
                     TVTTWTLPLPYPAIGLPQTDLPLAVLVDGGVNSAAEGLAGALQARGRATVVGERSAGN
                     VEALLPFCLRDGSQAWVAAGVLAPIGGPTWEGRGVEPDVEADPDEALPVAIATLLRLE
                     DER"
BASE COUNT          379 a         1088 c         1080 g          403 t
ORIGIN      
        1 tgtgatcggt taccgcagca ccagggcgcg ccggtcgggt gcgccggtcg tctcgttcga
       61 cgaggccatg ctcgctgggt tggccccgga cggcgggctc tactggccca cgcggatagc
      121 gccgttgccg ttgggctggg agaccgcgtc gtcgccggcc gagctggcgc tggcagtcct
      181 gccgccgtac gttggagacg aagccgccgc cgccatagct ggcggtctcg acttcgcctg
      241 gccggtcagc cgcctatcgg acgacgtctt cctcctcgag ctcttccgcg gcccgacggc
      301 cgccttcaag gacgtcgggg cccgctccct cgccagggcc atggatgccg ctctggccga
      361 caggggagag cgcgtcaccg tcctcacggc cacgtcgggc gacacgggca gcgccgtggc
      421 cgacgctttc gccggcctgg agcgcgtccg cgtcgccgtg ctctacccgg ccggcggcgt
      481 cagcgcggtc caggaggcgc agttgacggc ggccaggccg ggcgtcagga cgttcgccgt
      541 cgccggcacg ttcgacgact gccaacgcct cgtcaaggcc gcgttcgccg acgaggggct
      601 gcgcggcacg cggctctcgt ccgccaactc gatcaacgtc ggcaggctgc tgccccagct
      661 cctgtactac ctatggggca cggcgcggac ctgggaggcg tcgggtcgtc gtcagccgct
      721 cgagatcgtc gtcccaagcg gcaacctcgg taacctcact gccgggatgc tggcggccga
      781 ggccggcatg cccgtggcgg gcttcctggc ggcgcacaac cgcaacgact tcttcccgcg
      841 ccacctggcc ggtgaggccg acgcctacgc cttcagcggg agcgtgagca cgccgtctaa
      901 cgccatggac gtaggggcgc cgtcgaactt cgagcggttg tacgaggggt tcggcgacga
      961 cctgcggagc cgggtcgcgg cggtcagggt ggacgacgcg gccacgttcg agcgcatgcg
     1021 gctcacctac gagcgccacg gcgcgctcgt ctgcccgcac accgcggtcg gcctcgaggc
     1081 gctggaactc caccgcgccg cggcgcggtc tctcaccggg tcccgcgccg gcgccggtga
     1141 cgcgagcgcc ggcgcgcatg agcgggggcg gtcgacgccg gcgctggtcc tcgccaccgc
     1201 ccatccggcc aagttccccg agtccgtgct caaggcgacg ggtgtcacgc cacccgtgcc
     1261 ggccaccctg gaacgtttcg agcgcatgcc gaagcgggtc gaggggctgg cggccgagcc
     1321 ggcggcgctg ggggccgcgc tcctcgactg gaccgagtga gcggtcgcca cgacagggtc
     1381 ggagtacggt agcggaccgg gcccgccggg acggctagcg cctgacggcg aacgggccgc
     1441 gctcgtctga cccaaggtgc acgaccctga gctcgcgcat cagcttggcg acgtgcgcga
     1501 gcacgctctt ctcggcgaac gcgagcacgg actctgggtg gtccgggtag acgcggcgtg
     1561 ttatctccat cgcgtcggcc ggtccgagct cgagggccgc caggatctgc gcttctcgcg
     1621 ccaagcggtg ctcggcgagc tcgcgtagcc gagccgtcgg cgcggtgatc gccggcccat
     1681 ggccggcccc gaggaccggg gcgcccagtg cggcgaggcg ttccaggctg gtcaggtagt
     1741 cgccgacgtc gccctccggg agcccaaccc acacggagcc ggaggccgac gccaagtcgc
     1801 cgacgacgat cgcctcgggc gaggcgttgc ccgccttcgt gacggcgaac gagaggtggc
     1861 cgtgactgtg gcccggcgtg tgcatggccc gaacgtcgac atcgccgacg gtcagaacac
     1921 gcccgtccgc caggaacgtc gcttgccact ctcccggcaa ctcggccgcc tccgccgcgt
     1981 gcgcgtagac gggcggcgac gagcccgtcc gggacgcttc cgacctgagg agcgacggca
     2041 cgccgcccag gtggtcgggg tgcgtgtggg tgagcagcac gcccttcacg aggcgcacgt
     2101 cggcggcggc cagagccgag gccagcagcg cgtcggcctc gggccggtcg gaaccggggt
     2161 cgatcaccac cccgacgccg cccttcgcca ctatgtacgc gttcacatgg tcgtagggcg
     2221 gcagcgtatc gcacggcagt tcgacgcgcc agacgtgctc ggagagtcgt tctaccggcc
     2281 gcgtcaccgc tcgtcctcca gccgcaacag cgtcgcgatg gcgacgggta gcgcctcgtc
     2341 agggtcggcc tcgacgtccg gttcgacgcc gcggccctcc catgtcgggc cgccgatggg
     2401 cgccagcacg ccggccgcca cccacgcctg cgagccgtcg cgcaggcaga acggtagcag
     2461 cgcctcgacg ttgccggcgc tgcgctcgcc gaccaccgtc gcgcgcccgc gcgcctgcag
     2521 cgcgccggcg agcccctccg ccgcgctgtt gacgccgccg tcgaccagca ccgccagggg
     2581 caggtcggtc tggggcaggc cgatggccgg atagggcaga ggcagcgtcc aggtcgtgac
     2641 ggtgcgccat agcaggccgc tcgtgaacac tccggccacc tgcatcatcg tcacgagcct
     2701 gccgcccggg ttgccgcgca ggtcgaggac gatggctgaa gccccctcgg cctggacgtt
     2761 cttcacggcg ttcctgacgg cagccgccgt gcccgtcgtg gcgaggtcgg gcaaccggac
     2821 gtagccgacc ccctctggcg tgaggccgta ggtcacgttc gcctcgtgtc cggcagcgcg
     2881 caagtcgctc ggcaggcgct gtccgagcag cgcgggcgcg ccgggcgcgt acgtcaggcc
     2941 cggcgcgccg
//
LOCUS       DAOQYE010000149         2944 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_36015_length_2944_cov_2.806854, whole genome shotgun sequence.
ACCESSION   DAOQYE010000149 DAOQYE010000000
VERSION     DAOQYE010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36015_length_2944_cov_2.806854"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="PKN52_03470"
     CDS             <1..254
                     /locus_tag="PKN52_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99033.1"
                     /translation="LVVHMRHIIHIKELASWDPGRILILQTVVAVVAANAVVQDRILE
                     PGVVAVVMGRPVEMGHYWADLNMAEAEEYITMPASRMRI"
     gene            complement(512..1693)
                     /locus_tag="PKN52_03475"
     CDS             complement(512..1693)
                     /locus_tag="PKN52_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HNQ99034.1"
                     /translation="MPYRFETLQIHAGQENVEATTKSRAVPVYQTVAYNFDSAEHAGR
                     LFRLQEFGNIYSRIMNPTNDVLERRIAALEGGLAAVATASGHAAEFLTFTTLAEAGDN
                     IVSSPNIYGGSHNMLGITLPRLGIEGRFVGGEDDPAEYERLIDDKTKVVYLESVPNPS
                     LRIPDIRTIAEIAHARGVAVVVDNTCGAGGYLFRPGELGADIVIHSATKWINGHGTAL
                     GGLVVDMGSFDWGNGRYPGFSEPCPSYSGITFSETFGKGSPFGNIAFAIRARVETLRD
                     QGQALAPQNAFLLLQGLETLSLRVDRHVENTRKLVEWFAKQPEVESVNYPELPGNPSY
                     ERAKEWFKRGAGAVFTFNIKGGLKAGQTFIDNVKLASRLVNLGDAKTSVTHPASTSVS
                     V"
     regulatory      complement(1977..2098)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(2212..2490)
                     /locus_tag="PKN52_03480"
     CDS             complement(2212..2490)
                     /locus_tag="PKN52_03480"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNQ99035.1"
                     /translation="MTDEFEKFQADLLASVEQMKSGQAARVTEVELSAVAAARVQIGL
                     SQSEFAGLLGVSPRTLQDWEQGRREPTGAARTLLKVAVSHPEVLRDLH"
     gene            complement(2521..>2944)
                     /locus_tag="PKN52_03485"
     CDS             complement(2521..>2944)
                     /locus_tag="PKN52_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99036.1"
                     /translation="LQTIELKKLCVSGGQDFQLSPAGVTHALVNTVAAFDMTKTPVSA
                     ILASSDDLVRRLSVDKTDVRPGDALGYYLYVSDKVTVRGTCVGADGLEVTYELGANAG
                     WNLVAYEFEAAGDGVRGVLRTVRSFPTGMGWVSLVNYD"
BASE COUNT          507 a          859 c         1024 g          554 t
ORIGIN      
        1 tgctggtggt tcatatgcga catattattc atatcaagga actagcgagc tgggatccgg
       61 gacgaatact tatactgcaa acggtggtgg cggtggtggc ggccaatgca gtggtacagg
      121 acagaattct ggagccgggg gtggtggcgg tggttatggg tcggccggtg gagatgggtc
      181 attattgggc ggatctcaat atggcagagg cggaggagta tataacgatg ccggcctcac
      241 gaatgagaat ataagctatc gcaaaggtgc aggaggcggt ggcggttgct caggtattca
      301 aatgcaagct ggatatggag gtgctggcgg tggagttatt aatatccaaa caaaaacctt
      361 agttgtgcag ggacagatta gttgtgatgg taatgacggt cagaatgcgc aagcttataa
      421 cactagtttt agagcaggcg gaggtggtgg aggatctgga ggcactatat ttattgtttg
      481 tttattaggc gcaacaatcg gaagtaattt attacacgct aacggaggtg ctggcgggat
      541 gggtgacgga tgtcttggca tcgccgaggt tcacgaggcg cgaggcgagc ttcacgttgt
      601 cgatgaaggt ctggcccgcc ttgaggccgc ccttgatgtt gaacgtgaag acggcgccgg
      661 cgccgcgctt gaaccactcc ttggcccgct cgtagctggg gttgcccggc agttcggggt
      721 agttgacgct ctcgacctcg ggctgcttgg cgaaccactc gacgagcttg cgggtgttct
      781 cgacgtgcct gtcgacgcgc aggctcaggg tctcgaggcc ctgcaggagg aggaaggcgt
      841 tctgcggtgc gagggcctga ccctggtcgc gcaaggtctc cacccgggcc cggatggcga
      901 acgcgatgtt gccgaacggc gagcccttcc cgaaggtctc ggagaacgtg atgccggagt
      961 agctggggca cggttcggag aagccggggt agcggccgtt gccccagtcg aacgagccca
     1021 tgtcgacgac gaggccaccg agggccgtgc cgtggccgtt gatccatttc gtggcggagt
     1081 ggatgacgat gtccgcaccc agttccccgg ggcggaagag gtagccgcca gcgccgcacg
     1141 tgttgtcgac gactaccgcg acgccgcgag cgtgcgcgat ctcggcgatg gtgcggatgt
     1201 cggggatgcg caggctcggg ttggggacgg actccaggta gacgaccttc gtcttgtcgt
     1261 cgatcaggcg ctcgtactcc gccgggtcgt cctcgccgcc cacgaagcgc ccctcgatgc
     1321 cgaggcgcgg cagcgtgatg ccaagcatgt tgtgcgagcc gccgtagatg ttgggcgagg
     1381 agacgatgtt gtcgcccgcc tcggccagcg tggtgaaagt caggaactcg gccgcgtgcc
     1441 cgctggcggt cgccaccgcc gccaacccgc cctcgagggc cgcgatacgc cgctcgagga
     1501 cgtcgttcgt ggggttcatg atgcgcgagt agatgttgcc gaactcctgc aggcggaaga
     1561 ggcgcccggc gtgctcggcg ctgtcgaagt tgtaggcgac cgtctggtac acgggcaccg
     1621 ccctcgactt cgtggtggct tcgacgttct cctggccggc gtggatctgc agcgtctcga
     1681 aacggtatgg catctctctg ggtctccttt agaccgcggc cttggcggcc gcatacaagt
     1741 tagggggttc gttgcgactt gtgaacacgc gcactagcgg ttcgctagtg cgaatgtgat
     1801 atccacaccc cgtggtacca ggccagcggc cacccgaggg cggttggggt gcgctgcggc
     1861 ctacgccgcg aggggcattc gccgaccggg ccgggcgtga acgcgctgcg tgcgagcgtg
     1921 aagttcaacg ggtagatgtt ggtcgtttag aagcatgcca gccctcctct acggaaccgt
     1981 tatcttcccg gtggctcacc atcgtggctg cgccccggct ggagttagca cccgcctgaa
     2041 tgaaggctgg ttgctgcggc gtcgtggggc caggtccctc ggccgctctg gataaggccg
     2101 tcggcgcgta agcgctgggc ggctcagatc gcgggcgcgg ctagcgcgcc cgagcgattg
     2161 gtcagtacaa catacacgcg cccctcgttg caagcagcgg ggctgggcgc ctcagtgaag
     2221 gtcgcgcaag acctctgggt ggctcaccgc caccttcaag agcgttctgg ccgcgccggt
     2281 aggctcgcgg cgtccttgct cccagtcctg gagggtacgc ggtgaaacgc ccagaagccc
     2341 ggcgaactcg ctctgcgaca agccgatctg cacccgcgcc gccgccacag ccgacagctc
     2401 gacctcggtt acccgcgcgg cctgcccact cttcatctgc tccacggagg caaggagatc
     2461 cgcctggaac ttctcgaact cgtcagtcat cctctattcc tatcaggaaa ccaacccacg
     2521 tcagtcgtag ttgacgagcg acacccagcc catgccggtc gggaagctac gcaccgtgcg
     2581 caggacgccg cgcacgccgt cgccggcggc ctcgaactcg tacgccacga ggttccagcc
     2641 ggcgttggcg cccaactcgt acgtgacctc caggccgtcg gcgccgacgc acgtgccgcg
     2701 gaccgtgacc ttgtcgctca cgtagaggta gtacccgagc gcgtcgccgg gccgcacgtc
     2761 cgtcttgtcg accgacagac ggcgcacgag gtcgtcgctc gaggcgagga tggccgagac
     2821 gggcgtcttg gtcatgtcga aggccgccac cgtgttgacg agcgcgtggg tcacgccggc
     2881 gggcgagagc tggaagtcct gtccgccgga gacgcacagc ttcttgagct cgatggtctg
     2941 gagg
//
LOCUS       DAOQYE010000150         2943 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_36031_length_2943_cov_3.473338, whole genome shotgun sequence.
ACCESSION   DAOQYE010000150 DAOQYE010000000
VERSION     DAOQYE010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2943
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36031_length_2943_cov_3.473338"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(327..674)
                     /locus_tag="PKN52_03490"
     CDS             complement(327..674)
                     /locus_tag="PKN52_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99037.1"
                     /translation="MSSRVFVKAFHSLAVIVFAIAACYLIYCGVTGVRDAIMVWALVA
                     VAIETVVYVGFGRKCPLTMLARWLGDEGGHDYLFEWLLGARNIGLVSRSLAGLAAVGV
                     LLIIIGSVRQALA"
     gene            902..1723
                     /locus_tag="PKN52_03495"
     CDS             902..1723
                     /locus_tag="PKN52_03495"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HNQ99038.1"
                     /translation="MPSTPSASSTGRGPAHRPVHRSVAAATVVTLGGHEVELVRKRVK
                     RVTLRIYPPDGRVRLTAPGNTPLRDLARLVAERSDWIERHQKRFSELAVGAAARYLPG
                     ETHYLRGIAYTLARPAAPGGAGASRVRIHTVGDRIVLHAPADADLAALERAFARFYRN
                     ELASDLAALVTKWERGLGVKASAVGIKRMSTRWGSCNTVAGRLWFNLELAKRRPSLLE
                     CVVVHELVHLLVADHGPRFKALMTRHLPEWRALSRELEAWPLWARLPSQDARTAP"
     gene            complement(1744..2859)
                     /locus_tag="PKN52_03500"
     CDS             complement(1744..2859)
                     /locus_tag="PKN52_03500"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNQ99039.1"
                     /translation="MDNVPVWGSRGWEPLPALRGDVEADVCVVGLGGSGLSAVRELLA
                     LGRSVVGVDAGGVAGGAAGRNGGLLLAGLAHFHHDARVRLGAERAVALYRVTMAGMEA
                     ELVAAGASARRVGSLRIAADAAEEADCEEQYAVMRADGLPVERYEGSEGTGLLFPDDA
                     VFDPLERCRRLARQALASGARLFERSQVVELGGGRVRTVAGSVACDSIVVAVDGRLAK
                     LLPELAGEVKDVRLQMLATAPTSEVSLPMPVYRRYGYEYFQQLPDGRLALGGFRDRGG
                     DAEWTDVAEPSARVQDLLEEYLREGLGVRAPITHRWAATVGYTDTYLPVAREARAGVW
                     AIGGYSGTGNVVGAVLGRAAAHRAVGLPSAEWDLFAS"
BASE COUNT          470 a         1082 c          990 g          401 t
ORIGIN      
        1 ccggcgaacg gctgcagcat gttctcgaac tgttggaaga ggtcgtcgac gccgcccatc
       61 ggggcgaggt agcgggcgac aggacggtta cgcaaagcgt tgctcttgac tagtgccatg
      121 gtgtcccttt ccgtggcggt catggtctct cgggcttcgg tcgggttggc ctggccgggc
      181 cgaggagatc ggccttggcc tcgcccacgc ccgtcagcgt ggccgaagcc ttgcgggtcg
      241 gcgcggcctg agcctaagcc accgccactg cttagggaag catagaactt gagtgtcagc
      301 ttgtcaagtt aactgctcgc acgccctcag gccaaggcct gcctcacgct gccgatgatg
      361 atgagcagca cgcctacggc cgccaaaccg gccagcgagc gcgacacgag cccgatgttg
      421 cgggcgccga ggagccactc gaacaggtag tcgtgcccgc cctcgtcgcc gagccaacgc
      481 gcgagcatcg tcagcggaca cttgcggccg aagccgacgt agacgacggt ctcgatggcg
      541 acggccacga gcgcccacac catgatggcg tcccttaccc ctgtcacgcc gcagtagatc
      601 aggtaacaag cggcgatggc gaagacgatg acggcgagac tgtggaaggc tttgacgaag
      661 accctggaag acacgaccaa gggggtcggt cgcggcctgg gtaggtacca tctcggttgc
      721 gacaccttga cctccgtcgg ctccgaacgg cgctagttca cacgaccgga accaagtggc
      781 ccagtgtact ccgccatccc gcgccttcgg taggcaggcg tcgtggcgtt gcgctcggcc
      841 agggcgcggt cagcgaccgc aagcgttgct ccgcgccccg gacaagtgcg acagtggcgg
      901 catgcccagc actccgtcgg catcctcgac cggccgcggc cccgcgcacc ggcccgtgca
      961 ccgctcggtc gcggcggcga ccgtcgtcac cttgggcggc cacgaggtcg agctcgtcag
     1021 gaagcgcgtc aaacgcgtca ccctgcgtat ctacccgccg gacggccgcg taaggttgac
     1081 ggcgccgggc aacaccccgc tgcgcgacct cgccaggctc gtggcggagc gcagcgactg
     1141 gatagagcgg caccagaagc ggttctcgga gttggctgtc ggcgccgcgg cgcgctatct
     1201 gcctggcgag acgcactact tgcggggcat cgcctacacg ttggcgcgcc ccgcagcgcc
     1261 gggtggagcg ggcgccagca gggtcaggat ccacacggta ggcgaccgca tcgtcctaca
     1321 cgcgccggcg gatgcggatc tcgccgcgct ggagcgcgcc ttcgcacggt tctatcggaa
     1381 cgagctcgcc agcgacctcg cagcgctcgt caccaagtgg gagcgaggac tcggcgtgaa
     1441 agcgagcgcg gtgggcatca aacggatgtc gacgcgctgg ggcagctgca acaccgtcgc
     1501 cggtcgcctc tggttcaacc tcgagctagc caagcgccgc ccaagcctgc tcgaatgcgt
     1561 ggtggtgcac gaactggtgc acctgctggt cgccgaccat ggtccgcgct tcaaggcgct
     1621 catgacgcgg cacctgccgg agtggcgcgc cctcagccga gaactcgagg cgtggccgct
     1681 ctgggcgcgg ttgccaagcc aagatgcaag gaccgcaccc tgaggcgtcg gcaccgcgcg
     1741 gcgctaggac gcgaacaggt cccactccgc cgatggcagc ccgacagcgc ggtgagccgc
     1801 cgcccgcccc agcacggccc cgacgacgtt gccggtgccg ctgtagccgc ctatggccca
     1861 cacccccgcg cgcgcttccc tggcaacggg caggtaggta tccgtgtagc cgaccgtcgc
     1921 agcccagcgg tgggtgatgg gcgcacgcac tcccagcccc tcgcgcaagt actcctcgag
     1981 caggtcctgc acccgcgcgg acggctctgc gacgtccgtc cactcggcgt cgccgccccg
     2041 gtccctgaag ccgcccagcg cgagccggcc gtcgggtagc tgctggaagt actcgtagcc
     2101 gtaccggcgg tagacgggca tcggcaggct cacctcgctc gtcggagccg tggccagcat
     2161 ctgaaggcgc acgtccttca cctcccccgc cagttccggc aggagcttgg ccagccgtcc
     2221 gtcgacggcc acgacgatag agtcgcacgc gacactgccg gccacggtgc gcacccgccc
     2281 gcctccaagc tccacgacct ggctgcgctc gaacagccgc gctcccgagg ccagcgcctg
     2341 acgcgccaag cgccggcagc gttcgagcgg gtcgaagacc gcgtcgtcag gaaagagcag
     2401 ccccgtaccc tcggacccct cgtaacgctc aacgggcaga ccgtcggcgc gcataacggc
     2461 gtactgctcc tcgcagtcgg cctcctctgc ggcgtcggcc gcgatgcgca gggagccgac
     2521 gcggcgcgcg ctcgcgccgg ccgcgaccag ttccgcctcc atcccggcca tggtcactcg
     2581 gtagagcgca accgcgcgct ccgccccgag ccggacccgc gcgtcgtgat ggaagtgggc
     2641 cagcccggcc agcaggaggc cgccgttgcg cccggccgct ccgccggcca cgccgccggc
     2701 gtccaccccg acgacgctgc ggccgagggc caggagttcg cgcacggcgc taaggccgga
     2761 ccctcccagc cccaccacgc agacgtcggc ctcgacgtcg ccccggagcg cggggagggg
     2821 ctcccagccc ctgcttcccc agacggggac gttgtccatg gctgctcctc tcacgccagg
     2881 cgcctgccac gcgggatcga ccaactcagc aggcagatga gcacggcgat cacgaacacg
     2941 ccg
//
LOCUS       DAOQYE010000151         2941 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_36069_length_2941_cov_2.626819, whole genome shotgun sequence.
ACCESSION   DAOQYE010000151 DAOQYE010000000
VERSION     DAOQYE010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2941
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36069_length_2941_cov_2.626819"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1040)
                     /locus_tag="PKN52_03505"
     CDS             complement(<1..1040)
                     /locus_tag="PKN52_03505"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNQ99040.1"
                     /translation="MNNTKPSTRSGGKPTIKHVAAEAGVGVGTVSRVLNEEQGVSSAM
                     RERVLRVAKDLGYARSSVGRSLKTGMTGNVGVAIMSRHAPVILNPFYAVVIGGIEEVL
                     EEHDRHLLLSSLRRKGHLLDLAKEGRVDALLVVGCDIDSTDLELLKGTGRPVVLVDHS
                     HPGLPSVTVDHVAAGRLAAEHLMERGCRRPAFVSENLENPNFRMRLQGFKAVLDEHGV
                     KLSATGIAAGGDSWEGGYHAMNLVLASGVTPDGVFAANDPAALSAMRALNEAGLKVPQ
                     DVKIVGCDDIHLASQSQPPLTTLAIEKRRLGQLGAELAIELLAGEAVGAGAATNGAGG
                     ERMAGAIEGGRA"
     gene            complement(1263..1658)
                     /locus_tag="PKN52_03510"
     CDS             complement(1263..1658)
                     /locus_tag="PKN52_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99041.1"
                     /translation="MNLRLIRMGTWLILIVLAVWLYAGRRVEPNYVSIALRHVPEGCT
                     AVGAWRDAGSGAGEKGAGETFVLCNEGRVQHMLRVLDRTPKEVDVVDVDFFGANVGPS
                     PAAFALNPDGSLSVRDDLGQVRTVKKVDF"
     gene            complement(1714..>2941)
                     /locus_tag="PKN52_03515"
     CDS             complement(1714..>2941)
                     /locus_tag="PKN52_03515"
                     /inference="COORDINATES: protein motif:HMM:NF014801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HNQ99042.1"
                     /translation="STQIGDLERRFGQDRFFERTGGSINQQVLAPKLRWLAEHEPGVF
                     ERAEVFCGSYDYIAYRLTGQLFAEHNWALESGLLDIATNAWSDELLVLGGINATRVPS
                     VRASHEIVGEVSRSAAVETGLPAGTPVIAGVADHVASAFAAGASGAGDLLLKYGGAGD
                     ILYSLGSLKTDPRLFIDHHVVPGLYYLNGCMATSGSLVKWFAANFCSADEAAARQAGQ
                     PLYAYLDQQAAALPPGSDGLVLLPYFLGEKTPLHDTFARGTVVGLGLNHGRHHLFRAV
                     LEGVAYGFRHHVDVLQELSLEPARILASDGGAASDLWLQITADVIGRPVQRVIGHPGS
                     SLGAAFVAGKAVGLFDDWSEVGRFVSLSEPFEPRDAARAAYDEYYALYRETYRRLVDM
                     YPRLVGVRGRAGSLTI"
BASE COUNT          471 a         1017 c          977 g          476 t
ORIGIN      
        1 tccgcgcgcc cgccttcgat ggcccccgcc atgcgctccc ctccggcgcc gttcgtcgcg
       61 gcgcccgcgc ccaccgcctc cccggccaga agctcgatgg cgagctcggc gccgagctgt
      121 ccgagccggc gcttctcgat ggcgagcgtc gtcagcgggg gctgggactg gctggccagg
      181 tggatgtcgt cgcaacccac gatcttcacg tcctgcggca ccttcagacc ggcctcgttg
      241 agggcgcgca tggcggagag ggcggccggg tcgttggccg cgaacacgcc gtccggcgtc
      301 acgccgctcg ccaggaccag gttcatggcg tgatagccgc cttcccagct gtcgccgccc
      361 gcggcgatgc ccgtggcgct cagcttcacg ccgtgctcgt ccagcacggc cttgaagcct
      421 tggaggcgca tgcggaagtt cgggttctcg aggttctcag agacgaaggc cggacgccga
      481 cagccgcgct ccatgaggtg ctcggccgcg agcctgcccg ccgcaacatg atcaacggtc
      541 acggacggca gccctgggtg cgagtggtcg acgaggacga cggggcgccc cgtacccttg
      601 agcagctcga ggtcggtcga gtcgatatcg caacccacca ccagaagggc gtctaccctt
      661 ccctccttgg cgaggtcgag caggtggccc ttgcgccgca ggctgctgag gaggaggtgg
      721 cggtcgtgct cctcgaggac ctcctcgatg ccgccgatga cgaccgcgta gaacgggttg
      781 aggatcacgg gggcgtgcct cgacatgata gcgacgccga cgttgcccgt catgccggtc
      841 ttgaggctgc gccccacgct gctccgcgcg tagccgaggt cctttgcgac ccgcaggacg
      901 cgctcgcgca tggcggacga cacgccctgc tcctcgttga gcacgcgcga cacggtaccg
      961 acacccacgc cggcttccgc cgcgacgtgc ttgatggtcg gcttgccgcc cgacctggtg
     1021 ctcggcttcg tgttgttcat cctgctcgcg cctttcagct tggtgacccc ggtggctgtg
     1081 agcgaagcgg tcgcacgcag gcgcgcgttg gtacgctcgc gtcggctgga aacgtttcca
     1141 acagattagg gcaacgggtg gctcgtgtca aaccgtgtcc ggctttttgg ctgtgcgtct
     1201 gtctctgcgg tggcgcgcga gttccttggg gcgacggaga atgacgccga gtgccggaac
     1261 cgctagaagt cgaccttctt cacagtcctg acctggccga ggtcgtccct cacgctcaag
     1321 ctgccgtccg ggttgagggc gaacgccgcg gggctcggcc cgacgttcgc accgaagaag
     1381 tcgacgtcca ctacgtcaac ctccttcggc gtgcggtcta gaacgcgaag catgtgctgg
     1441 acccgaccct cgttgcacag cacgaacgtc tccccagcac ccttctcacc agcgccagag
     1501 ccggcatcgc gccatgcgcc gactgccgtg caaccctccg gcacgtgccg caacgcgatc
     1561 gagacgtagt tcggctcgac acgcctgccc gcgtacagcc acacggcgag gacgatcagg
     1621 atcagccatg tgcccatgcg gatcagacgt aggttcatag ctgaagatga caaaagacac
     1681 cggcctccac cttgttcgat caggcgggca agtctatatc gtcagactcc cagccctccc
     1741 ccgcactccc actaaccgcg gatacatatc caccagccgc cgatacgtct cccgataaag
     1801 cgcgtaatac tcgtcgtacg cagctcgcgc cgcgtcccga ggctcgaacg gctcggacag
     1861 gctgacgaat cttcctacct cggaccagtc gtcgaagagt ccgacggcct tccccgccac
     1921 gaaggcagcc ccgaggctcg agcccgggtg accgatcacc cgttgaacgg gtctcccgat
     1981 gacgtccgcc gtgatctgca accagaggtc gctggcggcc ccgccatcgg acgcgaggat
     2041 ccgcgccggc tccagggaga gctcctgcag cacgtcgacg tggtgcctga agccataggc
     2101 caccccctcg agcacggctc gaaagagatg gtggcgaccg tggttgagcc ccagtccgac
     2161 gacggtgccc cgcgcgaacg tgtcgtgcag cggcgtcttc tcgccgagga agtagggcag
     2221 gagcacgagc ccgtcgctgc ccggcgggag cgcggcggcc tgctgatcga ggtacgcgta
     2281 gagcggctgc ccggcttgcc gggcagcggc ctcgtcggcg gagcagaagt tggcggcgaa
     2341 ccacttcacg aggctgccgc tcgtcgccat acacccgttc aggtagtaga ggcctggcac
     2401 aacgtggtgg tcgatgaaga gccgcgggtc ggtcttgagg ctgcctaggc tgtagaggat
     2461 gtcgcccgcg ccaccgtact tcaggaggag gtccccggcg ccggaggcgc ccgccgcgaa
     2521 ggccgaggcg acgtgatcgg cgacgccggc tatgacgggc gtacctgcag gcagacccgt
     2581 ctcgactgcc gccgaccggc tgacctcgcc gacgatctcg tgcgaggccc gcacgctcgg
     2641 cacgcgggtg gcgttgatac ccccgaggac gaggagctca tccgaccagg cgttggtcgc
     2701 gatgtccagg agccccgact cgagcgccca gttgtgctcg gcgaagagct gcccagtgag
     2761 gcggtaggcg atgtagtcgt aagagccaca gaagacctcg gcgcgctcga agacgccggg
     2821 ttcgtgctcg gcgagccacc tgagcttcgg cgccagtacc tgttggttga tggacccacc
     2881 tgtacgctcg aagaagcggt cctggccgaa cctgcgttcc aggtcgccga tctgcgtgga
     2941 c
//
LOCUS       DAOQYE010000152         2940 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_36094_length_2940_cov_2.598614, whole genome shotgun sequence.
ACCESSION   DAOQYE010000152 DAOQYE010000000
VERSION     DAOQYE010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2940
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36094_length_2940_cov_2.598614"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..811)
                     /locus_tag="PKN52_03520"
     CDS             complement(<1..811)
                     /locus_tag="PKN52_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HNQ99043.1"
                     /translation="MAKAVGIDLGTTNSVIAVMEGSEPVVLVNAEGSRTTPSVVAFKG
                     EQRLVGQVAKRQAVLNPKGTLFEIKRYIGRTWDEVKSEAERAPYEVVRGDDGGVRFVV
                     EGKQYTPEEISAMILRKLVADASERLGSPITKAVITVPAYFNNSQREATQNAGRIAGL
                     EVLRIVNEPTAAALAYGLEKKGNETVLVFDLGGGTFDVSVLEVGDGVFEVRSTSGDTH
                     LGGSDMDYAIVQWLADDFQKEYNVDLRKDKQALQRLIEAAEKAKIELSGLPE"
     gene            complement(1011..2492)
                     /locus_tag="PKN52_03525"
     CDS             complement(1011..2492)
                     /locus_tag="PKN52_03525"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNQ99044.1"
                     /translation="MPRIPEPSEGWLERFDAFSGEFMTRHKVPGAAVTIVHEGETAYG
                     RAFGERDREAGAPAAMDTLFGLASVTKAFTALTLLNLEARGLLRLDDPVTRYLPSFTY
                     PGFVPEAPVRVWHLASHTSGLPPVRGLDYAVYPSQVGDPSDAYNTRDYTNAPRVDDYP
                     ALLAYLASGTRPALAHPGGVVSYSNDGYGLLGAVIEAATGEAYPAVVRREVLGPLGLG
                     AGFDTAEARATGRLTELYTSTPSGEVIHSPQWEEAPAYLATGFLKASADDLSAYLKYL
                     LAPAPGRLAVSPEAVGQLWRPRAWAEHLTSYGLGWMLRERFNGLTLRRHGGSLKGISS
                     HVGMVPELGLGIAVLTNLDEVPVKRLWFAAVNAYLGLPLGAAPFAHGAQPTTDEAELL
                     AGVYDSGEPWGRLELVRDGESLRALSGETASDIGVVELLPNGEFVVADETGPWDSGRF
                     VWDGAPVDPPSARGGGATEERGARVGKPGAVQFGMRWYDRTDE"
     gene            complement(2494..>2940)
                     /locus_tag="PKN52_03530"
     CDS             complement(2494..>2940)
                     /locus_tag="PKN52_03530"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNQ99045.1"
                     /translation="RSLHIPEVYSLLDLSRLGEKQEISAREEADRIAARLNERGIGSR
                     VRIEKGIPFREILRAEAEEDVSMIVIGSLGVAGAILSEATLSFLGLGIQEPLASWGTM
                     INRGQQYIFNAWWYSVFPGAVIMIVVLGFNFLGDAVRDALAVEEGR"
BASE COUNT          442 a         1001 c         1003 g          494 t
ORIGIN      
        1 tctcgggtag tccggagagc tcgatcttgg ccttctccgc cgcttcgatg agccgctgca
       61 gcgcctgctt gtccttgcgc aggtcgacgt tgtactcctt ctggaagtcg tcggcaagcc
      121 actgcacgat ggcgtaatcc atgtcggagc cgcccaggtg cgtgtcgccg ctcgtggagc
      181 gcacctcgaa cacgccgtcg cccacttcga gcacggagac gtcgaaggtg ccgccgccca
      241 ggtcgaagac gaggaccgtc tcgttgccct tcttctcgag gccgtacgcg agggccgccg
      301 ccgtgggctc gttgacgatg cggagcactt cgaggcccgc gatgcggccg gcgttctggg
      361 tggcctcgcg ctgggagttg ttgaagtacg cgggaacggt gatgacggcc ttggtgatgg
      421 gcgatccgag ccgctcgctc gcgtcggcga cgagcttgcg caggatcatc gcggagatct
      481 cctcgggcgt gtactgcttg ccctccacga cgaagcgcac gccgccgtcg tcgccgcgca
      541 cgacctcgta cggggcgcgc tcggcctcgc tcttcacctc gtcccacgtg cggccgatgt
      601 agcgcttgat ctcgaagagc gtacccttcg ggttgagcac ggcctgacgc ttggccacct
      661 ggccgacgag gcgctgctca cccttgaacg ccacgacgga gggcgtggtg cggctaccct
      721 ccgcgttcac gagcactacg ggctcgctgc cttccatcac cgcgatgacg ctgttggtcg
      781 ttccgaggtc tatgcctacg gcttttgcca ttctgggctc cttactcgat ctaggggaat
      841 cttttcgacg aagctcgtaa tcacgtcctg agcgctcgaa gcaagtcgcg cggcccggca
      901 cgggcttgtc actggcggga agtataccca gcctggcatt cccatgtcaa gagatgtgag
      961 tgtgctcata tcaactttga cgagggggtt cgcttaggac ccgccgatcc ctactcatcc
     1021 gtgcggtcgt accaacgcat gccgaactgc accgcgccgg gtttgccgac gcgcgcgccg
     1081 cgttcctccg ttgcgccgcc gccgcgcgcg gagggcgggt cgactggcgc gccgtcccac
     1141 acgaagcggc cgctgtccca cgggcccgtc tcgtcggcca cgacgaactc gccgttcggc
     1201 agcagttcga cgaccccgat gtcagacgcg gtctcgccgg agagcgctcg caggctctcc
     1261 ccatcgcgca cgagctcgag gcgcccccac ggctcgccgg agtcgtagac gcccgccagg
     1321 agctcggcct cgtcggtcgt cggctgtgcg ccgtgcgcga acggcgccgc gccgagcggc
     1381 aggccgagat aggcgttgac ggccgcgaac cagagacgct tcacgggcac ctcgtcgagg
     1441 ttcgtgagca ccgcgatgcc gaggcccaac tccggcacca tgccgacgtg cgacgagatg
     1501 cccttgagcg aacccccgtg gcgcctgagc gtcaggccgt tgaagcgctc gcgcagcatc
     1561 cagccgaggc cgtagctcgt gaggtgctcg gcccaggcgc gcggccgcca cagttgcccg
     1621 accgcctccg gcgagaccgc gagcctgccg ggcgcaggcg cgagcaagta cttcaggtag
     1681 gcgctcaggt catcggcgct cgccttgagg aagccggtgg cgaggtaggc gggcgcctcc
     1741 tcccactggg gcgaatggat gacttcgcca ctcggagtgc tcgtgtacag ctcggtgagc
     1801 ctgccggtgg cccgcgcctc ggctgtgtcg aagccggcgc caagcccgag ggggccgagc
     1861 acctcgcgcc tcaccaccgc agggtaggcc tcgcctgtcg cggcctcgat gacggcgccg
     1921 agaagcccgt aaccgtcgtt cgagtaactc acgacgccgc ccggatgcgc aagggccggc
     1981 cgcgtcccgg acgccaggta ggccagtagg gccgggtagt cgtccacgcg cggcgcgttc
     2041 gtgtagtcgc gcgtgttgta ggcgtccgac gggtcgccga cctggctggg gtatacggcg
     2101 tagtcgaggc ctcgcaccgg cgggaggccg ctggtgtggc tggccaggtg ccagacgcgt
     2161 acgggagcct ctggcacgaa gcccgggtag gtgaacgacg gcaggtagcg cgtgaccggg
     2221 tcgtcgagcc tcaacaagcc ccgcgcctcg aggttgagga gggtgagggc cgtgaacgcc
     2281 ttggtcacgg aggccaggcc gaagagcgtg tccatggcgg ccggcgcgcc ggcctcgcgg
     2341 tcgcgctcgc cgaaggcgcg cccataggcc gtctcgccct cgtggacgat ggtcacggca
     2401 gcgcccggca ccttgtggcg ggtcatgaac tcgccggaga aagcgtcgaa gcgctccagc
     2461 caaccttcgg acggctctgg gatccgcggc acctcagcgc ccctcttcca cggccagggc
     2521 gtcgcgcacc gcgtcgccga ggaagttgaa gccgagcacg acgatcatga tcacggcgcc
     2581 cgggaacacg gagtaccacc aggcgttgaa gatgtactgc tgaccgcggt tgatcatggt
     2641 gccccaactg gcgagcggct cctggatgcc gaggcccagg aaggagagcg tcgcctccga
     2701 gaggatggcc ccggccacgc caagggagcc gatgacgatc atcgatacat cctcttccgc
     2761 ctccgctcga aggatctccc tgaaggggat gcctttttcg atcctgacac ggctgccgat
     2821 tcccctttcg ttgagtcgtg ccgctattct gtccgcctcc tcccgtgcgg agatttcctg
     2881 cttttctccc agccgggaaa ggtccaggag ggagtagacc tcgggaatgt ggaggcttct
//
LOCUS       DAOQYE010000153         2939 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_36109_length_2939_cov_2.813454, whole genome shotgun sequence.
ACCESSION   DAOQYE010000153 DAOQYE010000000
VERSION     DAOQYE010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36109_length_2939_cov_2.813454"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1079
                     /locus_tag="PKN52_03535"
     CDS             <1..1079
                     /locus_tag="PKN52_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99046.1"
                     /translation="RLRLGEPAAAEQQLQSYINLTGDDRDPEVRSLIGRAQWANGELE
                     AANGNIATAHQLRGVRNVAAQADDLRALAMIAYAQGDVDAGGLALRESLKRENLTSFL
                     RGNSLIWLLLLVLLVGAHLIGESRIASSSSLEVVDGPRNWSVGQVFTTLFAALLLALL
                     VTVVYGLVRFDNTLAIITPPLRSEMHALFVTVFAVLLALFAWRRVQANGFDAMETLLG
                     SSRSAGYGVGWGALFLVGTLAYLHYIDGGLGRFYLDFVRTSPFVVAAAFLVPLAEIYF
                     RPFVMTPLTRRYDGAIATIGSACLSAIAFGTPSLLLVVFGLLLADAYRRRRSGWEVLV
                     AQLTMHLGLLAAALLSPWVRALFF"
     gene            1111..2058
                     /gene="truB"
                     /locus_tag="PKN52_03540"
     CDS             1111..2058
                     /gene="truB"
                     /locus_tag="PKN52_03540"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HNQ99047.1"
                     /translation="MPVLPVDKPLGLSSHDVVARARRLLGTRRVGHAGTLDPLASGVL
                     VLLTEEATKLSPFLTASDKEYLAWVAFGLGTPTLDAEQPEDAATLTWAEPGATAALTR
                     ESVAVAARTFLDVTEQRPPAFSAIKQGGTRSYAAARRGETTEPPARPVAYRHVEVLAF
                     APLRGGLPANAGPDGRPFRLPPELAELPCALVSLTVAAGTYVRSFARDLGAALGVPAH
                     LAGLVRTRAGKVRLTDCSALEGVAGAAPLDPVALLPYPRIDLDAAAAVAVRQGKQVTL
                     GLTSTTALVDEDGALAAVVEPGAGGRLRYLRVWQRGLTP"
     gene            2265..>2939
                     /locus_tag="PKN52_03545"
     CDS             2265..>2939
                     /locus_tag="PKN52_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HNQ99048.1"
                     /translation="MNIGIPKEIKPQEYRVAATPGAVHALTSAGHRVLVEAGAGLGSS
                     FPDEEYLAAGAELGTVSDAWSQQLVLKVKEPIASEYGYLRPDLLLFTYLHLAADRALT
                     DALVASRTTAVAYETVQLANGTLPLLTPMSEVAGRMAPQVGAHFLERAHGGKGVLLAG
                     VPGVQTGVVTIIGAGVVGTNSAKISLGLGARVNLLDVSHSRLQYLDDVVGGRVQTMAS
                     NLGNLRE"
BASE COUNT          398 a         1099 c          989 g          453 t
ORIGIN      
        1 tgaggctgcg ccttggcgaa ccggcggcgg ccgaacagca gttgcagagc tacatcaact
       61 tgacgggcga cgatcgcgat cccgaggtgc gctcgctgat aggtcgcgcg cagtgggcga
      121 acggcgaact cgaagccgcc aacggcaaca tcgccactgc ccaccagctc cgcggcgtgc
      181 ggaacgtggc ggcccaggcg gacgacttgc gcgccctcgc catgatcgcc tacgcgcaag
      241 gcgacgtcga tgccggtggg ctcgcgttgc gcgagtcgct caagcgcgag aacctgacgt
      301 cgttcctgcg cggcaactcg ctcatctggc tcctactgct cgtgctcctg gtcggcgctc
      361 acctcatcgg cgagagccgc atcgcctcgt cctccagcct cgaggtcgtg gacggcccac
      421 gcaactggag cgttgggcag gtcttcacga ccctcttcgc ggcgctgctg ctggcgctcc
      481 tcgtgacggt cgtctacgga ctcgtgcgtt tcgacaacac gctggccatc atcacgccgc
      541 cgctcagaag cgagatgcac gccctgttcg tgacggtgtt cgcggtgctg ctcgccctct
      601 tcgcctggcg ccgcgtccag gccaacggct tcgacgccat ggagacgctg ctcggcagct
      661 cgcggtccgc cggctacggc gtcggctggg gggcgctctt cctggtaggc acgctcgcct
      721 acctccacta catcgacggt ggcctcggcc gcttctacct cgacttcgtg cgcacgtcgc
      781 ccttcgtggt ggccgccgcc ttcctcgtac ccctggcgga gatctacttc cggcccttcg
      841 tgatgacgcc cctcacgcgc aggtacgacg gggccatcgc gaccatcggg tcggcctgcc
      901 tctcggcgat agccttcggc acgccgtccc tgctgctggt cgtcttcgga ctcctcctgg
      961 cggacgccta ccggcgccgc aggagcggct gggaagtgct cgtggcccag ttgaccatgc
     1021 acttggggtt gctggcagcc gcgctcctca gcccatgggt cagggcgctc ttcttctaac
     1081 cgatgctccg ccggccgtcc cgccgatagc gtgcccgtcc ttccggtcga caagccgctc
     1141 ggcttgagtt cgcacgacgt cgtcgcccgc gcgcgccgcc tgctcggcac gcgccgcgtg
     1201 gggcacgccg gcacgctcga cccgttggcc tccggcgtcc tggtcctcct caccgaagag
     1261 gcgacgaagc tgtcgccgtt ccttaccgcc agcgacaagg agtacctggc ctgggtcgcc
     1321 ttcgggctcg gcacgcctac cctggacgcc gagcagccgg aggacgcggc gacgctgacg
     1381 tgggcggagc cgggggcaac ggccgcgctg actcgcgaga gcgtcgcggt ggccgcacgc
     1441 accttcctcg acgtcacgga gcagcgccca cccgccttct cggccatcaa acaaggcggc
     1501 acgcggagct acgcggcggc taggcgaggg gaaacgacgg agccacccgc ccgcccggtc
     1561 gcctaccgcc acgtcgaggt cctcgccttc gcccccctgc gcggggggct acctgcgaac
     1621 gccgggccgg atgggcggcc attccgtctc ccgccggagt tggccgaact gccctgtgcg
     1681 ctcgtgtcgc tgacggtggc ggccgggacg tacgtgcgct ccttcgcccg cgacctcggc
     1741 gcggcgctcg gcgtgcccgc gcacctggcc gggctcgtca ggacgcgagc cggcaaggtc
     1801 cgactgacgg actgctcggc gctcgagggc gtggccggcg ccgcacccct agaccccgtc
     1861 gcgctcctcc cctacccacg catcgacctg gacgccgcgg cggcggtggc ggtacggcaa
     1921 ggcaagcagg tgacgctagg ccttacgagc accacggcgc tcgtcgacga ggacggggcg
     1981 ctcgcagccg tcgtcgagcc tggcgcgggc ggccgcctgc gctacttacg cgtctggcag
     2041 cgcggactca caccgtgatc ctgcgccgcc agcaggcgta ggcccggcgc gatcacacgg
     2101 ccgtcagagt ccggttccgc gccggccacg gtggcgcgcg gtgtcaccaa gtgcgctgct
     2161 tgccactcct gagggggcac gcgagtagtt agactcggga ggttgtctct cacaagtacc
     2221 gagtgggtcg gcgcatcgcc gtcggcccca cagggagctg aaagatgaac atcgggatcc
     2281 ccaaagagat caagccgcag gagtaccgcg tggccgccac gccgggggcg gtccacgccc
     2341 tcacgagcgc gggccaccgc gtcctcgtcg aggccggcgc aggccttggc tccagcttcc
     2401 ccgacgagga gtaccttgcc gccggcgccg agctgggcac cgtctccgac gcctggtcgc
     2461 agcagctggt gttgaaggtg aaggagccca tcgcgagcga gtacggctac ctgcgccccg
     2521 acctgctcct cttcacctac ctgcacctgg ccgccgaccg ggccctcacg gacgccctcg
     2581 tggcgagccg caccacggcc gtggcctacg agacggtcca actggccaac ggcacgctgc
     2641 cgctcctcac ccccatgagc gaggtggcgg gccggatggc gccgcaggtc ggcgcccact
     2701 tcctcgagcg cgcccacggc ggcaagggcg tgctgctggc cggcgtgccg ggggtgcaga
     2761 caggtgtcgt gaccatcatc ggcgccggcg tggtcggcac caactccgcc aagatctcgc
     2821 tcggcctcgg tgcgcgcgtc aacctccttg acgtcagcca ctcgcgtctg cagtacctgg
     2881 acgacgtcgt cggcggccgc gtccagacca tggcgagcaa cctcggtaac ctccgcgag
//
LOCUS       DAOQYE010000154         2938 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_36126_length_2938_cov_3.417621, whole genome shotgun sequence.
ACCESSION   DAOQYE010000154 DAOQYE010000000
VERSION     DAOQYE010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2938
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36126_length_2938_cov_3.417621"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            406..1644
                     /locus_tag="PKN52_03550"
     CDS             406..1644
                     /locus_tag="PKN52_03550"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNQ99049.1"
                     /translation="MMRPIRYVVLLLAAAFLTSALAQTNLTFWSWRTEDVAAYEKFIA
                     EFHAQNPDINVTFTPYLNTEYNTIVSTALQGGGGPDIVHLRAYGGMEPLAQAGLLVRL
                     DDKVGALAGFDPGILLGATNRADGGVYGVPFALQTVQVLYNVDMFENLGLSVPTTWTE
                     FLAVGDALKASGVYALANGAKEPWTLETLFGGVAPTFYGCTPFFQEITAGKTNFLDAR
                     FTGALQRMLDLRPYMADNYMGVDYTDMQILFAFGQAGMLVGGSYELGNLKNLNPDLKV
                     GAFAVPGDAAGDQSCISYYVDGSYGINAASSHQAEALKFIEFLASQQYGQMFTDELQQ
                     ISAVPGTAPTSEALANIVDLMNAKGTPYLLLTAFRFGQPSGSTLLQNEMQAAMAGDKT
                     VDDVAANIQRGVAEWFEAFK"
     gene            1768..2697
                     /locus_tag="PKN52_03555"
     CDS             1768..2697
                     /locus_tag="PKN52_03555"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNQ99050.1"
                     /translation="MHTKRSWWSKNGHLVMFLAPAVIIYGLFMAYPLISSLTYSLFEW
                     DGFVRRGFAGFKNFVTVLTRWPYNERFFSALSHNAYFFIVTFVVQTTLGLFVAVLLAR
                     KARGFAAFQAIYFLPHTLSLVVVGFLWRMLLNPTWGALSQALKRMGLADAVQPWLGQS
                     ATALTAVILVNGWAWLGFPILVFLAAVQAIPREFTEAAVVDGANAWQNFWHVTWPLLL
                     PSIAMLTILTFIWDFNAFELVFVMQGASGAPAYSTDLLATFFYRTAFGDPTTGGEPGQ
                     IGLAAAIGVFMLVIIGAVSVLGVRFMGRRQAEL"
     gene            2699..>2938
                     /locus_tag="PKN52_03560"
     CDS             2699..>2938
                     /locus_tag="PKN52_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99051.1"
                     /translation="MRSRALPTTFIYLFLALLALVFLYPVILMVLTAFKTTPEIFRNP
                     FGLPEALSWKGFRDVWERAHFGLYLRNSVIITALVI"
BASE COUNT          486 a         1023 c          894 g          535 t
ORIGIN      
        1 catcgtcgag cagagcctcc gggcggcggc cctctgggac gaagtgaagg acgacagcgt
       61 gaggtcgatg gctttctgca tccggggcct cgtctttcgt gggaccgcgg cgtgtgggat
      121 tgctgggggc tacgccggcg gccatgtccg ctcgagctaa actcggacgt tcgtgtccag
      181 gttatagctc cgagacccgg cgtttgggat tgacctgggt caaagttcgg gacgggtgtc
      241 ccttgacgtt ccgcccgctc gcgacacgcc gggcacgttg actaaagtgg tacaagaatg
      301 gtattaatgg cggtgcgaca caagagcggc ctcgagccac cgtcggtagg gatgccggcg
      361 gggttcctgc cgcaagaatc gttgtcccgt agaaggagtg cacgcatgat gcgacccatc
      421 cgatatgtcg tactgctgct tgcggcggcg ttcctgacgt cagccctcgc gcagacgaac
      481 ctcaccttct ggagttggcg cacggaggac gtggcggcgt acgagaagtt catcgccgag
      541 ttccatgccc agaaccccga cataaacgtc accttcacgc cgtacctcaa cacggagtac
      601 aacaccatag tctccacagc cctccagggc ggcggcgggc cggacatcgt gcacctgcgc
      661 gcctacggcg gcatggagcc cctagcgcag gcaggcctcc tcgtacgctt ggatgacaag
      721 gtgggcgcgc tcgccggctt cgaccccggc atcctcctcg gtgccaccaa ccgggccgac
      781 ggtggtgtct acggcgtgcc gttcgcgctc cagacggtac aagtgctcta caacgtcgac
      841 atgttcgaga acctcggcct gagcgtcccc acgacgtgga ccgagttcct cgcggtcggc
      901 gacgcgctca aggcctccgg cgtctacgcc ctcgccaacg gcgccaaaga gccgtggacg
      961 ctagagacgc tgttcggcgg cgtggcgccc acgttctacg gctgcacgcc gttcttccag
     1021 gagatcacgg ccggcaagac gaacttcctc gacgcccgct tcaccggcgc cctgcagcgc
     1081 atgctcgacc tgcgccccta catggccgac aactacatgg gcgtcgacta cacggacatg
     1141 cagatcctgt tcgccttcgg acaggccggc atgctggtcg gcggctccta cgagctcggc
     1201 aacctgaaga acctgaaccc cgacctcaag gtgggcgcct tcgccgtccc gggcgacgcg
     1261 gccggagacc agagctgcat cagctactac gtcgacggct cctacggcat caacgccgcg
     1321 agcagccacc aggccgaggc actcaagttc atagagttcc tcgccagcca gcagtacggc
     1381 cagatgttca ccgacgagct gcagcagatc tcggccgtgc ccggcaccgc gccgacgagc
     1441 gaggccctcg cgaacatcgt cgacctcatg aacgccaagg gcacgcccta ccttctcctg
     1501 acggccttcc gcttcggcca gccgagcggc tccacgctcc tacagaacga gatgcaggcc
     1561 gccatggccg gcgacaagac cgtcgacgac gtcgccgcca acatccagcg cggcgttgcg
     1621 gagtggttcg aagccttcaa gtgaacggct gacctttacg tcggacccgg ggcgccgcga
     1681 gcctagcggc gccccgtcct tagcgcgaga cccacgcaca caagaccgac gcacacgaga
     1741 ccgccgaagc atgagaagga ggcgagcatg cataccaagc gcagctggtg gtccaagaac
     1801 ggccacttgg tcatgttcct ggcgccggcc gtgatcatct acggcctgtt catggcttac
     1861 ccgctcatct cctcgctcac gtacagcttg ttcgaatggg acggtttcgt gcggcgcggc
     1921 ttcgccggct tcaagaactt cgttaccgtg ctcacgcgct ggccgtacaa cgagcgcttc
     1981 ttctccgcgc tctcgcacaa cgcctatttc ttcatcgtca ctttcgtcgt ccagacgacc
     2041 ctcggtctct tcgtggccgt gctgctcgcc cgcaaggcac gcggcttcgc cgccttccag
     2101 gccatctatt tcctgccgca tacgctctcg ctcgtggtcg tcggtttcct ctggcgcatg
     2161 ctcctcaacc cgacctgggg cgccctctcg caggcgctga agcgcatggg gctagccgac
     2221 gcggtccagc cctggctcgg gcagagcgcc accgcactga cggctgtgat cctcgtgaac
     2281 ggctgggcgt ggctcggctt cccgatcctc gtcttcctgg ccgccgtcca ggcgatcccc
     2341 agggagttca ccgaggccgc tgtggtggac ggcgcgaacg cctggcagaa cttctggcac
     2401 gtgacgtggc cgctcctgct gccgtccatc gccatgctga cgatcctcac gttcatctgg
     2461 gacttcaacg ccttcgaact cgtgttcgtc atgcaagggg cgtccggcgc gccggcgtac
     2521 tctaccgacc tcctcgccac gttcttctac cggacggctt tcggggaccc gaccaccgga
     2581 ggagagccgg ggcagatcgg gctggcggcg gccatcggcg tgttcatgct cgtgatcatc
     2641 ggcgcggtgt ccgtgctcgg cgtgcgcttc atgggccgta ggcaggcgga actctgacat
     2701 gcggagccgc gcactaccga ccacgttcat ctacctgttc ctcgctctgc tcgcgctcgt
     2761 cttcctgtac cccgtcatcc tgatggtcct cacggccttc aagacgacgc cggagatctt
     2821 ccgcaacccg ttcggcctcc cggaggcgct ctcctggaaa ggcttccggg acgtgtggga
     2881 acgcgcgcac tttgggctct acctgcgcaa cagcgtcatc atcacggccc tggtgata
//
LOCUS       DAOQYE010000155         2922 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_36450_length_2922_cov_2.483432, whole genome shotgun sequence.
ACCESSION   DAOQYE010000155 DAOQYE010000000
VERSION     DAOQYE010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2922
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36450_length_2922_cov_2.483432"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..599)
                     /locus_tag="PKN52_03565"
     CDS             complement(<1..599)
                     /locus_tag="PKN52_03565"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HNQ99052.1"
                     /translation="MPTTKLLMSGEELAAALKAGDPRLRIVDARFELSDPAFGRQAYA
                     AGHVPGAIHLDLDHDLAGPKGAHGGRHPLPDMEAFAAKLGAAGIGDEHLVVVYDHAGG
                     MYAGRAVWLLRFAGHEDVRLLDGGLNAYQAAGGELTTVEPRYAPATLTVRLRPEMVVD
                     ADYVKAAIGRDDVVLLDARAPERYRGETEPLDPKAGHIPG"
     gene            639..1493
                     /locus_tag="PKN52_03570"
     CDS             639..1493
                     /locus_tag="PKN52_03570"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNQ99053.1"
                     /translation="MATRHVESAANPFVKELAALKDRRERERSGTYLVEGQREAGRAL
                     DARVDTVRIVVAPSLLPTGSSMETWRERAGASGAELWTVSPSAFRRLSIRENPDGVVL
                     VVRTRRTPPAGVPLAEGALVLVLAGLEKPGNVGALLRSADAFGADAVFMCPDADGPDG
                     EQGGGKGVDLENPNLVRAAMGSSFASTVGVGTREEVLAAVRAAGLRLVATTPSATTSV
                     WECDLTAGAAILLGREHEGLSDWWLARADVQVQVPMRQTAADSLNVSVTGALLLFEAS
                     RQRHGGAG"
     gene            complement(1464..1865)
                     /locus_tag="PKN52_03575"
     CDS             complement(1464..1865)
                     /locus_tag="PKN52_03575"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNQ99054.1"
                     /translation="MSEPERKTTILVADDAEGQRMVLEMLLSVDGYEVVTVEDGKAAL
                     EYLKDHTPDLAILDVMMPHLSGLQVCHRMKRIPRLKDVPVLVLTAMRDEETLTHARMA
                     HADGVVFKPLEGKDFRETVRNLLAGATMPLP"
     gene            1941..2366
                     /gene="mscL"
                     /locus_tag="PKN52_03580"
     CDS             1941..2366
                     /gene="mscL"
                     /locus_tag="PKN52_03580"
                     /inference="COORDINATES: protein motif:HMM:NF010557.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HNQ99055.1"
                     /translation="MIKEFRDFINRGNVLDLAVAVIIGGAFGAITTSLVNQVIMPVIG
                     LILGGADFSNLGIIMKDADKYASVADAVAAGAPVLQYGAFLNTVINFFLIAIVLFFIV
                     KAFNQAKKAFEKPAEPAAPAEPSAEVKLLTEIRDAMKQR"
     gene            complement(2404..>2922)
                     /locus_tag="PKN52_03585"
     CDS             complement(2404..>2922)
                     /locus_tag="PKN52_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99056.1"
                     /translation="PAAIREELAAFINDATAFVARRGVERVRVTTEQFNVLVDEIGQT
                     PGPDLVRLISPSNQISSTIDVLVEAIENTELYAKGQLVVSHGLHLGSVELPASQGEIR
                     TSIAQLKADAVRELRRAGLDDGQLPDFGPVTEEMFTNQLLRLVGPVTIGFVATEEVWR
                     AGPAKLELMILY"
BASE COUNT          449 a          974 c         1020 g          479 t
ORIGIN      
        1 ccgggtatgt ggccggcctt cggatcgagc ggctcggtct cgccgcgata ccgctcaggc
       61 gcgcgggcat ccagcaggac cacgtcgtcg cgaccgatgg ccgctttcac gtagtccgcg
      121 tcgacgacca tctccggacg cagcctgacg gtgagggtgg cgggggcgta gcgcggctcc
      181 accgtggtga gctccccacc ggcagcctgg taggcgttca ggccgccgtc caggagccgc
      241 acgtcctcat gcccggcgaa gcgcagcagc cacacggccc gcccggcgta catccccccg
      301 gcgtggtcgt acacgaccac caggtgctcg tccccgatgc cggccgcgcc gagcttggcc
      361 gcgaacgcct ccatgtcggg gagcgggtgc ctaccaccgt gagctccctt cgggcccgcc
      421 aggtcgtggt cgaggtcgag gtggatggcc cccgggacat ggccggcggc gtaggcctgc
      481 ctaccgaagg ccgggtccga gagctcgaag cgcgcgtcga cgatgcgtaa ccgagggtcg
      541 ccggccttga gggccgcggc gagttcctca ccggacatga gaagcttggt cgtgggcatg
      601 cgggagtgta ctcccgaggc tacgcatatg atgcccgaat ggccacgaga cacgtggaga
      661 gcgccgccaa ccccttcgtg aaggagctcg cggcgctgaa ggacaggcgc gagcgcgagc
      721 gcagcggcac gtacctcgtc gaagggcagc gggaggcggg ccgcgcgctg gacgcgcggg
      781 tcgacacggt gcgcatcgtg gtggcgccgt cgctactgcc gacagggtcg agtatggaga
      841 cgtggcggga gcgtgccggg gcgagcggcg ccgagctctg gaccgtcagc ccgagcgcgt
      901 tcaggcggtt gagcatccgc gagaacccgg acggcgtcgt actcgtcgta cgtacgcgcc
      961 ggacaccgcc cgcgggcgtc ccgctggccg aaggagcgct cgtgctcgtc ttggcagggc
     1021 tcgagaagcc tgggaacgtt ggcgcccttc ttaggagcgc cgacgcgttc ggcgccgacg
     1081 ccgtgttcat gtgcccggac gccgacgggc cggacgggga gcaagggggc ggcaagggcg
     1141 tcgacctgga gaacccgaac ctcgtcaggg ctgccatggg gagttcgttc gcctcgacgg
     1201 tcggggtcgg cacgcgcgag gaggtcctgg ccgccgtccg cgcggcaggg ctgcgtctgg
     1261 tggccaccac cccttcggcc accaccagcg tgtgggagtg cgacctcacg gctggcgccg
     1321 ccatcctgct cgggcgcgag catgaggggc tgtccgactg gtggctggcc agggcggacg
     1381 ttcaggtgca ggtacccatg cgtcagacgg ccgccgacag cctcaacgtg tcggtgacgg
     1441 gcgccctgct cctgttcgaa gcgtcaaggc agcggcatgg tggcgccggc tagcaggttg
     1501 cgcaccgtct ccctgaagtc cttaccctcg agcggcttga agactacccc gtcagcgtgc
     1561 gccatgcgcg cgtgtgtgag ggtctcctcg tcgcgcatgg ccgtcagcac gagcaccggc
     1621 acgtccttca gtcgcgggat ccgcttcatc ctgtgacata cctgcaaccc gctcaggtgc
     1681 ggcatcatca cgtcgaggat ggccaggtcg ggcgtgtggt ccttcaggta ttcgagggcg
     1741 gccttgccgt cctcgacggt cacgacctcg tacccgtcga ccgataggag catctcgagg
     1801 accatccgct ggccttctgc gtcgtccgcg acgaggatag tcgtctttct ctccggctcg
     1861 ctcacgccac aagcttacgt aatctcgcac cctcgtgggg cctgaccgtt agactcgacc
     1921 ggaagttccc aggaggcttc atgatcaagg agttccgcga tttcatcaac cggggcaacg
     1981 ttctcgacct ggccgtcgcc gtcatcatcg gcggcgcctt cggcgcgatc accacctcgc
     2041 ttgtgaacca ggtcatcatg ccagtgatcg gactgatcct cggcggggcc gacttcagca
     2101 acttgggcat cataatgaag gacgccgaca agtacgcgag cgtcgccgac gccgtcgcgg
     2161 ccggcgcccc cgtcctccag tacggggcgt tcctgaacac cgtcatcaac ttcttcctga
     2221 tcgccatcgt gctcttcttc atcgtcaagg ccttcaacca ggccaagaag gcgttcgaga
     2281 agcccgccga gccagcggcc ccggccgaac ccagcgcgga ggtcaagctc ctcacggaga
     2341 tccgcgacgc gatgaagcag aggtaggtct cgtttagtgc actttcggcc gcgccggcca
     2401 ggttcagtag aggatcatca actcgagctt ggccggcccg gcgcgccaca cttcctcggt
     2461 ggcgacgaag ccgatcgtca cagggccgac gaggcgcagg agttggttgg tgaacatctc
     2521 ctcggtgacg ggcccgaaat cgggtagctg gccgtcgtcg agaccggcgc gacgcagttc
     2581 gcgcacggcg tccgctttga gctgagcgat ggacgtccgg atctcccctt ggctggccgg
     2641 cagttccacc gaccccaggt ggagcccgtg gctgacgacg agttggccct tggcgtagag
     2701 ctcggtgttc tcgatggcct ccacgagcac gtcgatggtc gagctgatct ggttgctggg
     2761 tgagatcagg cgcaccaagt cggggccggg ggtctggccg atctcgtcga ccaagacgtt
     2821 gaactgctcg gtggtgacgc gtacgcgctc gacgccgcgc ctggcgacga aggccgtcgc
     2881 gtcgttgatg aacgccgcca gctcctcgcg gatggcggcc gg
//
LOCUS       DAOQYE010000156         2915 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_36572_length_2915_cov_3.463986, whole genome shotgun sequence.
ACCESSION   DAOQYE010000156 DAOQYE010000000
VERSION     DAOQYE010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2915
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36572_length_2915_cov_3.463986"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..681
                     /gene="phnC"
                     /locus_tag="PKN52_03590"
     CDS             <1..681
                     /gene="phnC"
                     /locus_tag="PKN52_03590"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HNQ99057.1"
                     /translation="HFVVVVGLSGAGKSTLIRTVNNLVVPSSGRVVVNDVDVTHARGS
                     ALRAARSDIGMIFQTFNLVKRSSVIRNVLSGRLGDLPAWRGVLSAFPKADQAYSHDCL
                     ARVGIPEKAYVRADALSGGQQQRVGIARALAQRPSVMLADEPVASLDPPTSHAVMSDL
                     KRIAREDGITTMVNLHFIDMARDYADRIIGMRDGVVVFDGTPTEATDAAFEEIYGRPI
                     DDKDLRGA"
     gene            674..1459
                     /gene="phnE"
                     /locus_tag="PKN52_03595"
     CDS             674..1459
                     /gene="phnE"
                     /locus_tag="PKN52_03595"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HNQ99058.1"
                     /translation="MPDQAVALPRATGRVKSLVTLALVVALFVFAFGQTKFNLGALFT
                     GASDFFRFFGRMAPDLTALPQIWPPLLQTIQIAYVATIIGAIIAIPLVFLASANTAFD
                     PVSMWIARTLLTVLRSIPDLLWAALFVAVLGLGALPGVVALTLFSVGVLAKLGSETVE
                     SIDPGPLEALKAVGAGRNRTILFAVVPQIAATMVSYMLYVFEINVRASVVIGFVGAGG
                     IGVLLQTYMNFFDYPGLGTLILVIFIVVLAIDGLSVWARSRLI"
     gene            1466..2284
                     /gene="phnE"
                     /locus_tag="PKN52_03600"
     CDS             1466..2284
                     /gene="phnE"
                     /locus_tag="PKN52_03600"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HNQ99059.1"
                     /translation="MDTTRPVPTVVPPAPAHVRRNRTLLIGGVLLLVALFVLAIATMS
                     LPTWARVATGLQRNALPLLRGLVRPETAVFKLAWNSMVVTFFMAILGTALGALLAFPL
                     SFLAAGNLLGGTRWALPGKALLVAVRTFPEIVLAVIFVAAAGPGPTAGIMAMGIHSIG
                     YLGKVFSDVIEGIDQGPMEAIRAAGGNQLHTFLFAVVPQVLPEFASNVLYRFEINLRS
                     AAVLGMVGAGGIGLPLIQRLQFRRWAEVSMFLIVIVVFIVVIDALSSRIRRRLV"
     gene            2345..2779
                     /locus_tag="PKN52_03605"
     CDS             2345..2779
                     /locus_tag="PKN52_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99060.1"
                     /translation="MSDYPPAIRARLLDAAAEVLLATVTADRTGVIAYYREVMAAGRY
                     LYDAQRKYAHNALISDLFDHTSSEGEREGDDEPLTKERLLARLAQVGEALHDDEEAAE
                     FKTFLFELAERVAKASGGFWGGISDDEAAFLAELRRLLKMQG"
BASE COUNT          396 a         1098 c          910 g          511 t
ORIGIN      
        1 catttcgtcg tggtggtcgg cctgtcgggc gcgggcaagt cgaccctgat ccgcacggtc
       61 aacaacctgg tggtgccatc gtcggggcgc gtcgtggtga acgacgtcga cgttacgcac
      121 gcccgcggct ccgctctcag ggcggcgcgg tcggacatcg gcatgatctt ccagaccttt
      181 aacctcgtga agcgctcgag cgtcatccgc aacgtgctct ccggccgcct cggcgacctg
      241 ccggcctggc gcggcgtgct gagcgccttc ccgaaggccg accaggccta ctcgcacgac
      301 tgcctcgccc gcgtaggcat cccggagaag gcctacgtgc gggccgacgc cctctccggc
      361 gggcagcagc agcgggtcgg catcgcaagg gccctcgccc aacgcccgag cgtgatgctg
      421 gccgacgagc ccgtcgccag cctcgaccca cccacgtcgc acgccgtgat gtccgacctg
      481 aagcgcatcg cgcgcgaaga cggcatcacc acgatggtca accttcactt catcgacatg
      541 gcccgcgact acgccgatcg catcatcggc atgcgcgacg gcgtcgtcgt cttcgacggc
      601 acgcctacgg aggcgacgga cgccgccttc gaggagatct acggtcgccc catcgacgac
      661 aaggacctgc gcggtgcctg accaggcggt cgcgctgccg cgcgccacgg gtcgcgtcaa
      721 gtcgttggtg acgctcgcgc tggtggtcgc cctcttcgtc ttcgccttcg gccagaccaa
      781 gttcaacctc ggggcgctct tcacgggcgc ctccgacttc ttccggttct tcggccgcat
      841 ggcccctgac ttaacggccc tgccgcagat atggccgccg ctgctgcaga ccatccagat
      901 cgcctacgtc gctacgatca tcggggccat catcgcgatc ccgctggtgt tcctcgcgtc
      961 agccaacacg gccttcgacc ccgtgagcat gtggatagcc cgcacgctcc tgacggtgct
     1021 gcgcagcatc cccgacctcc tgtgggccgc cctcttcgtg gccgtgcttg gtctgggcgc
     1081 actcccaggg gtcgtcgcgc tcaccctctt ctccgtcggc gtgctcgcca agctcggctc
     1141 cgagaccgtc gagtccatcg acccggggcc gctggaggcg ctgaaagccg tcggcgccgg
     1201 ccgcaaccgg acgatcctct tcgccgtcgt cccacagatc gccgcgacca tggtctccta
     1261 catgctctac gtgttcgaga tcaacgtgcg cgccagcgtc gtcatcggct tcgtcggcgc
     1321 cggcggcatc ggggtgctgc tccagaccta catgaacttc ttcgactacc ctggcctcgg
     1381 caccctcatc ctcgtcatct tcatcgtcgt actcgccata gacgggctca gcgtctgggc
     1441 ccgctcgcgg ctcatctagc gcgccatgga caccactcgc cccgtcccca ccgtggtacc
     1501 gcccgcgccg gcgcatgtgc gccggaaccg cacgctgctc ataggcggcg tcctcctcct
     1561 cgtggccctg ttcgtcctgg ccatcgccac catgagcctg cccacctggg cgcgcgtcgc
     1621 caccgggctg cagcgcaacg cactaccgct cctgcgcggc ctcgtgcggc cggagaccgc
     1681 ggtcttcaag ctggcgtgga actcgatggt ggtcacgttc ttcatggcca tcctcggcac
     1741 ggcgctcggc gccttgctcg ccttcccgct gtcgttcctc gcggccggca acctgctcgg
     1801 cgggacgcgc tgggcgctgc cgggcaaggc cttgctggtc gccgtacgca cgttcccgga
     1861 gatcgtcctc gccgtcatct tcgtagccgc ggccggcccc ggccccacgg ccggcatcat
     1921 ggcgatgggc atccactcca tcggctacct cggcaaggtc ttctccgacg tgatcgaggg
     1981 gatcgaccag ggacccatgg aggccatccg cgccgccggt ggcaaccaac tccatacgtt
     2041 cctcttcgcc gtcgtgcccc aggtgctgcc agagttcgcc tccaacgtgc tgtaccggtt
     2101 cgagatcaac ctgcgctccg ccgccgtcct cggcatggtc ggcgcgggcg gcatcggcct
     2161 cccgctcatc cagcgcctgc agttccgccg ctgggccgag gtgtcgatgt tcctcatcgt
     2221 catcgtcgtg ttcatcgtcg tgatcgacgc gctcagctcg cgcatccgcc gccgcctcgt
     2281 ttagaggcgc cccctcgtgt gacccggagc agcgcgcggc ggcgcggcgt gcgatgatcg
     2341 cgccatgagc gactacccac cagccatccg cgcgcgcctg ctcgacgcgg cggcagaggt
     2401 gctcctggct accgtcacgg ccgaccgcac cggcgtgatc gcctactacc gcgaggtgat
     2461 ggccgctggc cgctacctct acgacgccca acgcaagtac gcgcacaacg ctctcatctc
     2521 cgacctcttc gatcacacgt cctcggaggg cgagcgcgag ggtgacgacg agccgctcac
     2581 caaggaacgg ttgctcgcgc gcctggcaca ggtcggcgag gcgctccacg acgacgagga
     2641 ggctgccgag ttcaagacgt tcctcttcga actggcggag cgcgtcgcca aggcgtccgg
     2701 cgggttctgg ggcggcatca gcgacgacga ggcggcgttc ctggcggagc tgaggcgcct
     2761 gctcaagatg caaggctgac cgccgtgaag gctcgctggg tctggggcct ggcgctcggt
     2821 tgcttcgtgc tggccgccgg cacgggcgcc tactaccgct tcaagctcgt ctacgcgctg
     2881 cccggcgtgc tcgagtacgt caggcacgcc cattc
//
LOCUS       DAOQYE010000157         2902 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_36819_length_2902_cov_2.086407, whole genome shotgun sequence.
ACCESSION   DAOQYE010000157 DAOQYE010000000
VERSION     DAOQYE010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2902
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36819_length_2902_cov_2.086407"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="PKN52_03610"
     CDS             complement(<1..542)
                     /locus_tag="PKN52_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99061.1"
                     /translation="MRAALLIARKDLRQRLRDRSFFIFGVIAPLALAYVFSLLLGPLA
                     RGVPDPPTIGLVDADRSAVSAGMRSMLDAVQAAGVITWREVAPSAGTPPPAGLVGAAA
                     DGLAGDARLVRVLSAYPDPASALEGEAYRALAARELDAAIVVPAGLLEALGRQPATVR
                     VLGMTNSLFGTQLAETLAGS"
     gene            complement(547..1506)
                     /locus_tag="PKN52_03615"
     CDS             complement(547..1506)
                     /locus_tag="PKN52_03615"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ99062.1"
                     /translation="MVSADPAREALLEVSDLKRSFGALQAVRGVSFTVGRGETYGLLG
                     PNGAGKTTTISMVAGILKRDGGSVTLGGEPITATSVGGRASIGLVPQDLAVYPDLNAR
                     ENLAFFGRLYGLGGRDLAERCDAVLETVGLLDRAAQLVREYSGGMKRRLNIAAGLLHR
                     PKLLILDEPTVGVDPQSRHAILQTIERLAGGGLAVLYTTHYMEEAERLCDRIGIVDDG
                     RLVAEGTRAELVRLVGGEDDVRVNAAGDLAAAAERVRGVPGVLRVDVTDDGLRLAANG
                     ASGLLTRLVHALDGPGIGVTGIEVREPDLEAVFLSLTGKALRD"
     gene            1727..>2902
                     /locus_tag="PKN52_03620"
     CDS             1727..>2902
                     /locus_tag="PKN52_03620"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNQ99063.1"
                     /translation="MRIRSILISLAALAYLTACGPQGGDTTPPALLSASPADGATDVA
                     LSETVVLTFSEPVLKLQVTPSPLVGLGAPAWDEARKVVTIAPSPAWPAGTALSFAIVA
                     EDDAGNSQSMSLGFSTLDDQEPPAAPTGLTSEAMDGAVRLAWTGGTEADLAGYLLFWG
                     DDPATPTGVVALPATATEHVLTPLENGTTYSVYLVAEDTAGNRSAPSATVTATPGDTT
                     PPTLVSSQPSDGLRGVGLVTRVRFVFSEPIEPGSLGLFLYQVQAPSGDDPLDPTAPVE
                     ATLDTALLGAPAWSPSGTVVEFSDVAPDLIESDKAYRFQLVAEDLAGNALVSPTTVSF
                     MAGFVPDVIPPEVTGFAQDIDPALGRGVLTFDFSEPMDQTATEAAFVSAPAMSCSWTW
                     "
BASE COUNT          418 a         1076 c          992 g          416 t
ORIGIN      
        1 aacgagcccg ccagggtctc ggcaagctgg gtgccgaaga gcgagttcgt catgcccaac
       61 acccgcaccg tggccggctg gcgccccagc gcttcgagga gcccagcggg cacgacgatg
      121 gcggcatcta gttcgcgcgc cgccagtgct cggtacgcct cgccctcgag cgcgctcgcg
      181 gggtccgggt aggcgctgag caccctgacc aggcgcgcgt cgccggcgag tccatcggca
      241 gcggcgccca ccaagccggc gggcgggggc gttccggccg acggcgcgac ttcccgccac
      301 gtgatgacgc cggcagcctg aacggcgtcc agcatggagc gcatgccggc cgatacggcc
      361 gaccggtccg cgtcgacgag tccgatggtc ggtggatccg gtacgccgcg cgccagcgga
      421 ccgagcagga ggctgaacac gtaagcgagg gcgaggggag cgatcacgcc gaagatgaag
      481 aaggagcggt cgcgtaaccg ctgccgcagg tccttgcgcg cgatgaggag ggcggctctc
      541 acttcgtcag tcccgcaacg ccttgcccgt taggctcagg aacacggcct cgaggtccgg
      601 ctcgcgcacc tcgatgcccg tgacgccgat gccaggaccg tcaagcgcgt ggacgagccg
      661 ggtcaggagt ccgctcgcgc cgttcgccgc caggcgcagg ccgtcgtccg tcacgtcgac
      721 gcgcaagacc cccggcacgc cccgcacccg ttcggccgcc gccgccaggt cgcccgcagc
      781 gttgacgcgg acatcgtcct cgccgcctac cagccgcacg agctccgccc gtgtgccctc
      841 ggctaccagc cgcccgtcgt cgacgatgcc gatgcggtcg cacaggcgct cggcctcctc
      901 catgtaatgc gtggtgtaga ggaccgcgag gccgccccca gccaggcgct cgatcgtttg
      961 gaggatggcg tggcgcgact gcgggtcgac tcccacggtc ggctcgtcga ggatgaggag
     1021 cttgggccta tggaggagcc ccgccgcgat gttgagccgg cgcttcatac cgcccgaata
     1081 ctcgcggacg agttgggcgg cccggtccag cagcccgacc gtctcgagaa ccgcgtcgca
     1141 acgctcggcg aggtccctgc cgccgagccc gtacaggcgt ccgaagaacg ccaggttctc
     1201 gcgggcgttc aggtccgggt acacggcgag gtcttgaggc acgaggccga tggacgcgcg
     1261 gcccccgacg ctcgtcgccg tgatgggctc gccgcccagc gtcaccgagc cgccgtcccg
     1321 cttgaggatg cctgcgacca tggagatggt cgtggtcttg ccggccccgt tcgggccgag
     1381 gagcccgtag gtctcgcccc ggccgaccgt gaacgacacg ccgcgcacag cctggagggc
     1441 gccgaagctc ctcttcaggt cgctgacctc caggagcgcc tcacgggcgg ggtcggcgga
     1501 taccacggtt aacaaggtag cagacggtcg tgagcgttgg aggttcgaag gcatggtgcg
     1561 ctcgcgggcc ggccgggccg acccgccgcg cctgatcgcc cgctgccggc gcgcctgccg
     1621 accctatgct tgtggcgccc ttgtggcacc cgcttcgcag gatgtgctta gggacactgc
     1681 tcggggccgc cccgccccac caatcgcgtc gagtgaggac gaaaccatgc gtatccgaag
     1741 catcctcatc agtctggcgg ccctcgccta cctcaccgcc tgcggaccgc agggcggcga
     1801 caccacgccg cccgcgctgc tctccgcctc gcccgccgac ggcgccacgg acgtcgcctt
     1861 gagtgagacc gtcgtcctga ccttcagcga gccggtcctg aaactgcagg ttacgccctc
     1921 ccctctcgtg gggcttggcg ctcccgcatg ggacgaggcg cgcaaggtgg tcacgatcgc
     1981 cccctcgccc gcctggccgg cgggcacggc gctcagcttc gccatcgtcg cggaggacga
     2041 cgccggcaac tcccaaagca tgagcctggg cttctcgacg ctcgatgacc aggagccgcc
     2101 ggccgcgccg accggcttga cgtccgaggc catggacggc gccgtccggc tcgcctggac
     2161 aggcggcacc gaagcggacc tcgcgggcta cctcctattc tggggtgacg accccgccac
     2221 gccgacgggc gtcgtcgcgc tgcccgccac ggcgaccgag cacgtgctga cgcctctcga
     2281 gaacggcacc acttactcgg tctacctggt ggccgaggac accgccggca accgctcggc
     2341 cccgtctgcg accgtcaccg ccaccccggg cgacaccacg ccgccgacgc tcgtctccag
     2401 tcaaccgtcc gacggcttga ggggcgtagg gcttgtcacg cgggtgcgct tcgtgttctc
     2461 cgagccgatc gagcccggct cgctgggcct gttcctgtac caggtgcagg cgccctccgg
     2521 cgacgatccc ctcgacccga cggcgcccgt cgaggccacg ctggacacgg cgctgctggg
     2581 cgctccggcc tggagcccga gtggcacggt ggtcgagttc agcgacgtgg cgcccgacct
     2641 gatcgagagc gacaaggcct accgcttcca actcgtggcc gaggacttgg ccggcaacgc
     2701 gctggtctct cccacgaccg tctcgttcat ggccggcttc gtgcccgacg tgatcccgcc
     2761 ggaagtgacc ggcttcgccc aggacatcga cccggctctg ggtcgcggcg tcttaacctt
     2821 cgatttctcc gagcccatgg accaaacggc tacggaggcg gccttcgtga gcgcacccgc
     2881 catgagttgc agttggacgt gg
//
LOCUS       DAOQYE010000158         2894 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_36975_length_2894_cov_3.321944, whole genome shotgun sequence.
ACCESSION   DAOQYE010000158 DAOQYE010000000
VERSION     DAOQYE010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36975_length_2894_cov_3.321944"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1226)
                     /locus_tag="PKN52_03625"
     CDS             complement(<1..1226)
                     /locus_tag="PKN52_03625"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF006006.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HNQ99064.1"
                     /translation="MDSSANRSGASDGQGAGSELGSEVFERSIVDLGAAMASGDLTAE
                     AAVQAYLERISALDQGGPRLGSVIEINPEALAIARELDDERRTKGPRGPLHGVPILLK
                     DNIDTADGTRTAAGSLALADSTPLQDSGVVARLREAGAVLLGKANMSEWANFRSTRSV
                     SGWSGRGGQCRNPYALDRNPSGSSSGSGAAAAASLCAAAVGTETDGSIVSPASHSGVV
                     GFKPTVGLVSRAGIIPIAHSQDTAGPMARTVEDAVLLLAAMQGPDERDPATAGISSGA
                     LVDPAAVLRSNGLQGARLGVVRNLVTGHPEMARQFEASLDALRASGAEVVDGLEMPRL
                     GEWRTAETEVLLYEFKHGLNAYLAGLPDRGQPRDLAELIEFNLANAERSMPIFGQELL
                     VAAQTKGPLSEPAYLEA"
     gene            complement(1399..>2096)
                     /locus_tag="PKN52_03630"
     CDS             complement(1399..>2096)
                     /locus_tag="PKN52_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99065.1"
                     /translation="ALPALLFALVGAFVGYSEFKRSAAAFEPLSSEILPAIGAAEWSV
                     TYLILALVTLRVIVQIGLIRRILNDLDLDIFNPSPIYTLSRYCAAYTATVLFTFHGLI
                     LLSLPTYYLSPSGLVFILALSAGALLTFVAPLTTINARLGRAKEALLCRIGEDQRRIN
                     DELHQAVSAASLGSLAELRIAVGVLKEQREVVERLPTWPWLGQTVRGVAPPLLVPVVV
                     YVAQRLLSLILGL"
     assembly_gap    2097..2106
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2121..2468)
                     /locus_tag="PKN52_03635"
     CDS             complement(2121..2468)
                     /locus_tag="PKN52_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99066.1"
                     /translation="MPTVPAYSPRWLWQTYRFFERLPVPYWLFSLIVVAALGVSKHLI
                     AWDLGLVPRGRINAYLGASGVYLVAVPFVWQLLNYHAVNAFAELRSKVEKQRLSVSLP
                     QFLSLPAVPARDA"
     gene            2622..>2894
                     /locus_tag="PKN52_03640"
     CDS             2622..>2894
                     /locus_tag="PKN52_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99067.1"
                     /translation="MTKKLPLLSSDPAKVAVALAFDLYGLKYNEPAFRFTPFPGPFEP
                     YVPVLPLITGYSIEDGRDVRGRTHPGLSAGERTRGRQRPPDAAPIDR"
BASE COUNT          528 a          944 c          961 g          451 t
ORIGIN      
        1 gcctccaagt aagcgggctc gctgagcgga cccttcgtct gcgcggcaac aagcagttcc
       61 tgcccgaaga taggcatcga ccgctcggcg ttggccaagt tgaactcgat gagttcggcc
      121 aggtcgcgcg gctgcccgcg atccggcagg ccggcaaggt aggcgttcag accgtgcttg
      181 aactcgtaga ggagcacctc ggtctcggca gtccgccact cccccagccg gggcatctcg
      241 agaccgtcga cgacctctgc gccggaggca cgcagcgcat ccagcgaggc ctcaaactgg
      301 cgtgccatct ctgggtggcc ggtgaccagg ttcctcacca cgcccagcct ggcaccttgc
      361 aaaccgttcg agcgcagcac ggcagccgga tcgacgaggg cccccgacga gatcccggcc
      421 gtagcagggt cgcgctcatc cggtccttgc atggcagcca gcagcagcac cgcgtcctcg
      481 acggtgcgcg ccatcggacc ggcggtgtcc tggctgtggg cgatggggat gatgccggca
      541 cgtgagacca gaccgacggt tggcttgaag ccgaccaccc cactgtggga ggcagggctg
      601 acgatggaac cgtccgtctc cgtgcccacc gcggcggcgc agaggctagc cgcggcggcc
      661 gcaccggagc cgctggacga accgctgggg ttgcgatcca acgcataggg gttcctgcac
      721 tggccgcccc ggccgctcca gccggagacc gaacgcgtag agcgaaagtt ggcccactcc
      781 gacatgttgg ccttaccgag cagtaccgcg cccgcttcgc gcagccgcgc cacgacgccg
      841 ctgtcttgca acggggtgga gtcggccaga gcgagggagc cggcggccgt acgagtgccg
      901 tcggcggtgt cgatgttgtc cttgagcagg atcggcactc cgtgcagcgg gccgcgcggc
      961 cccttggtgc gtcgctcgtc gtccagttca cgggcgatgg ccagggcttc cgggttgatc
     1021 tcgatgaccg agccaagacg agggccgccc tggtcgaggg cacttatgcg ctccaagtag
     1081 gcctggacgg ccgcctctgc ggtgaggtcg ccggaggcca tcgccgcgcc gaggtcgacg
     1141 atcgaacgct cgaagacttc gctgccgagt tcggagccag cgccctggcc atctgatgcg
     1201 ccactgcggt tcgcggaact gtccatgaag acctagtcta cgattgccga ccgcgttaca
     1261 cgggctggcg cccatgccag ttgcgcctgc cgactagtcc agaaacgccg actccgacgg
     1321 gatgatgctc gccggcccct gattggacgc gtgtggcagc ggtgcatctt ccctaagcaa
     1381 gcatgagagt cagtcggcct acaaccccag gatcaggctc aagagacgct gcgccacgta
     1441 aaccactacg ggcaccagca gtggcggcgc aactcctctg acggtctgcc ctagccaggg
     1501 ccaggtcggc agccgctcga ccacttcgcg ctgctccttc agcacgccga ccgcgatgcg
     1561 cagttctgcc aggcttccga gggaggcggc gctgactgcc tggtgcagct cgtcgttgat
     1621 ccggcgttga tcctcaccta tacggcatag caacgcctct ttggcccgtc ccaggcgggc
     1681 gtttatcgtc gtcagcgggg cgacgaacgt gagtagggcg ccggcgctca gagccaggat
     1741 gaagaccagc ccgctgggag acagatagta ggtaggcagc gacagcagga tgagtccgtg
     1801 gaaggtgaac aggaccgtcg cggtataggc ggcgcagtag cgcgacaggg tatagatcgg
     1861 actggggttg aagatgtcga ggtcgaggtc gttcaagatc ctgcgtatga ggcctatctg
     1921 gacgatgacc ctgagggtta ccaaggcgag gatgaggtag gtcaccgacc actcggcagc
     1981 gccgatcgcc ggtaggatct cggacgacag tggctcaaac gcggctgccg atcgtttgaa
     2041 ctccgagtag cccacgaatg cgcccacgag cgcgaagagc agcgcgggca gcgcggnnnn
     2101 nnnnnnatca acgcctcgat tcaggcgtcg cgcgcgggca cggccggcaa ggacaagaac
     2161 tgcggcaggc tgacgctcag ccgctgcttc tcgaccttgc tgcgaagctc cgcgaaggcg
     2221 ttgaccgcgt gatagttcag gagctgccag acgaacggca cggccactag gtagacgccg
     2281 gacgcgccga gataggcgtt gatccgcccc ctcggcacca gtccgaggtc ccaggcgatg
     2341 aggtgcttcg agacaccaag cgcagcgacc acgatcagcg agaacaacca gtatgggacc
     2401 ggtagccgct cgaagaagcg ataggtctgc cagagccagc ggggggagta ggcagggacc
     2461 gtgggcatag cggatcctcg tgggtcagag cagagtatag gaccgccgac cggtccggcc
     2521 gacctcaaag tcaccgtcgg acctagttgg caccctcgcc cgcctctact ccacccacac
     2581 gtacacggtc acgttaatcg gcctgtccgt accccgcgaa cttgaccaag aagctgccct
     2641 tgctcagttc cgacccggcg aaggtggccg tagcgttggc gttcgacctg tacggactca
     2701 agtacaacga gccggccttc cggttcacac cgttccccgg acctttcgag ccctacgtgc
     2761 cggtcttgcc gcttatcacc ggctattcca tcgaggatgg gcgagacgtc cgtggtagaa
     2821 cgcatcctgg tctgagtgcc ggcgaacgaa cgcgcggccg acagcgaccg cctgacgcgg
     2881 cacccatcga cagg
//
LOCUS       DAOQYE010000159         2891 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_37042_length_2891_cov_2.532793, whole genome shotgun sequence.
ACCESSION   DAOQYE010000159 DAOQYE010000000
VERSION     DAOQYE010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2891
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37042_length_2891_cov_2.532793"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /locus_tag="PKN52_03645"
     CDS             <1..651
                     /locus_tag="PKN52_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99068.1"
                     /translation="ALGCLIVAIAVLIVGSGLAYFYVIRPLTNTVKAGMELPRLAELD
                     QQVSNKRAFAAPESGELTAGQVDRYMQATSAVMNGLKGKADELAQKYDQISSQGNPGI
                     RQVLNAYTDIIKLVVQAKELQVDALNAAGFSLSEYDWVKRSVIRAAGQAYTVVDLTAL
                     SKGEVEEAADDAVADDAVAGDNAAGDAAAVPEANVELVKPYADAIEKYLPLVAFGL"
     gene            802..>2891
                     /locus_tag="PKN52_03650"
     CDS             802..>2891
                     /locus_tag="PKN52_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99069.1"
                     /translation="MAATYSSRGERQPAPRRTSVRRLGLVVLCVLGAALVGTAPAQQC
                     TYESEPNDDPAAATRLTDAGPQPFGPDRYNEVSARCLTGEVSGSDKDVYRWEVTELDA
                     RHRWTFSLQGPAGGVASTVLFRTDAAGVTTEYERVASQAGSAARSGEFLVEPGTYYLA
                     VSSGGAAGEYVVDLTPVIGLRYGAGEDRYDDGVGRRYTGAFGLFGPVAGELSQAFTID
                     ADGAKRVWGVELWAALGTEPRVELQGAGGVVTQGQVDAGGRVRLPGLQLAAGDYTLRV
                     LGDSGMVRLRLESQGVRGNGVAVEPNDDWGAATAFPLGTEMSATVGGRDFYRIDVSRE
                     GAGVYNLAFSADADLTYTLRDEAGNPLLVSRSGQPRTALAFAEGAYQLLVEGPDGTGY
                     RMALTPAAAPAGGEIEPNDYPITASPLPDGGQVRGRLDGSETDVYSLDVVDTAQRFRV
                     QLVGNSIDRLAELTSAGDVLNETRGQQRLRLDDVILLPGRHYFEVRGSAGDYALKVLS
                     LGPVQVGAEQPPTDQPLTPATQGSVGQDAAAAAAALESAVLEAGPPPPPGTLELEPND
                     DASRATRLVPGAVHVGRLTSESPDYYRFFLADDQYVSIEVVPPTGGSTIDLWFDDLGW
                     VRFPDLERGSAVKLERLFIAGDHTFYLDARNVETDGYYQLRVNLLGRLMPAVDAEPND
                     DYATASLMPAELEWT"
BASE COUNT          453 a         1019 c         1004 g          415 t
ORIGIN      
        1 gcgctcgggt gcttgatcgt ggccatcgca gtcctgatag tcgggtcggg cctcgcctac
       61 ttctacgtga tcaggccgct gaccaacacg gtgaaggctg gcatggagct gccgaggctg
      121 gccgagctcg accagcaggt cagcaacaag cgcgccttcg cggccccgga gagcggcgag
      181 ctcaccgcgg gacaggtcga ccgctacatg caagccacaa gcgccgtcat gaacgggctg
      241 aagggcaagg ccgacgagct cgctcagaag tacgaccaga tcagctcgca gggcaacccc
      301 ggcatacggc aggtgctcaa cgcctacacg gacatcatca agctcgtcgt gcaagccaag
      361 gagctgcagg tcgacgcgct caacgcggcc ggcttctctc tctcggagta cgactgggtc
      421 aagcgctcgg tgatccgggc ggccgggcag gcttacacgg tggtcgacct gacggcgctg
      481 tcgaagggcg aggtggagga ggcggctgat gacgccgtgg ctgatgacgc cgtggccggt
      541 gacaacgcgg ccggtgacgc cgcggcggta ccggaagcca acgtcgagct ggtcaagcca
      601 tacgccgacg ctatcgagaa gtacctgcct ttggtggcct tcggcctatg acgggggcgc
      661 ggcaagcgtg gtggaacgct caccgtgtac gccccatcgc ggtgcaagct gatctggtgg
      721 gcccgagcat cgggcacgcc gaacgacctg gtctttagcg cgccgcccgg ccccgaccgc
      781 gaggcgcttt aggaggagca cgtggcagca acgtacagct cgcgtggaga acggcagccg
      841 gcgccacgcc gcacgagcgt ccggcgcctc ggtctggtcg tgctgtgcgt gctcggtgcc
      901 gcgctggtcg gcacggcccc cgcccagcag tgcacgtacg agtccgaacc caacgacgat
      961 ccagcggcgg ccacgcggct tacggacgcc ggcccgcaac cgttcgggcc ggatcgttac
     1021 aacgaggtga gcgcacggtg cttgacgggc gaggtctcgg gttccgacaa ggacgtctac
     1081 cggtgggaag taacggagct cgacgcccgg caccgctgga ccttctcctt acaggggccc
     1141 gcgggaggcg tcgccagtac cgtgctgttc cgcacggacg ctgccggcgt gacgaccgaa
     1201 tacgagcgcg tggcgagcca ggccggcagc gcggcgcgct ccggtgagtt cctcgtcgag
     1261 ccgggcactt actacttggc cgtctcctcc ggcggcgccg ccggcgagta cgtcgtcgac
     1321 ctgacgcccg tcatcggcct gcgctacggc gccggagaag accgctatga cgacggcgtc
     1381 ggcaggcgct acaccggcgc gttcgggctg ttcggccccg tcgccggcga gctgtcccag
     1441 gccttcacga tcgacgcgga cggcgccaag cgtgtttggg gcgtggagtt gtgggccgcc
     1501 ctcggcaccg agcctcgggt cgaactgcag ggtgctggcg gcgtggtgac gcaagggcag
     1561 gtggacgcgg gtggacgcgt gcggctgccg gggctccaac ttgccgcagg tgactacacg
     1621 ctccgcgtgc tcggcgacag cgggatggtg cgcttgcgcc tcgagtcgca gggcgtgcgc
     1681 ggcaacggcg tggccgtcga gcccaacgac gactggggcg ccgccaccgc cttcccgctc
     1741 ggcaccgaga tgagcgcgac cgtcggcgga cgcgacttct accgcatcga cgtgtcccgg
     1801 gagggggccg gcgtctacaa cctcgctttc tcggccgacg ccgaccttac gtacacgttg
     1861 cgagacgaag ccggcaaccc cctgctggtc tcccgctccg gccaaccgcg tacggcgctc
     1921 gcgttcgccg agggcgcgta ccagctcctc gtcgaaggcc ctgacggcac cggctatcgc
     1981 atggccctca cgccggcggc ggcgccggca ggcggtgaga tcgagcctaa cgactacccg
     2041 atcaccgcca gcccactgcc ggacggcggc caggtgcgcg ggcgccttga cggctcggag
     2101 accgatgtct acagcctgga cgtcgtcgac accgcacagc gcttccgcgt ccagttggta
     2161 ggcaactcga tcgaccggct ggccgagctc accagtgccg gcgacgtgct gaacgagacg
     2221 cgtggccagc agcgcctccg gctggacgat gtgatcctcc tgccgggcag gcactacttc
     2281 gaggtgcgcg gctccgccgg cgattacgcc ctgaaggtcc tgtcgctcgg cccggtgcag
     2341 gtgggcgccg aacaaccgcc caccgaccag ccgctcacgc ccgcgaccca aggcagcgtc
     2401 ggtcaagacg cggccgccgc ggcggcggct ctggaatccg ccgtgcttga ggccggtccg
     2461 ccgccgccgc ccggcaccct ggaactggag ccgaacgacg acgccagccg cgccacccgg
     2521 ctcgtgccgg gcgccgtgca tgtcggacgc ctgaccagcg agagccctga ctactaccgc
     2581 ttcttcctcg cggacgacca gtacgtgagc atcgaggtcg tgccgccgac cggcggatcc
     2641 accatcgatc tctggttcga cgacctcggc tgggtgcggt tccccgacct cgagcgcggt
     2701 agcgccgtca agctcgagcg cctcttcatc gccggcgacc acaccttcta cctggacgcg
     2761 cgcaacgtcg agacggacgg ctactaccag ctcagggtca acctgcttgg ccgcctcatg
     2821 cccgccgtcg atgccgagcc caacgacgac tacgccaccg cgtcgctcat gccggccgag
     2881 ctcgagtgga c
//
LOCUS       DAOQYE010000160         2882 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_37205_length_2882_cov_4.043509, whole genome shotgun sequence.
ACCESSION   DAOQYE010000160 DAOQYE010000000
VERSION     DAOQYE010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2882
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37205_length_2882_cov_4.043509"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(103..747)
                     /locus_tag="PKN52_03655"
     CDS             complement(103..747)
                     /locus_tag="PKN52_03655"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF016362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HNQ99070.1"
                     /translation="MREHPTHLSEADIRPFARPGDEWWIKRGASENKANAENLQAGEL
                     DPTEQVEEGIRRIREGVKVELLSRLLDQDAAFFERAVVKLLVSMGYGGTGGVATVTNL
                     VGDGGIDGVIDQDVLGLSKVFVQAKRYAQDRAVQRPEVQAFVGALSGKAEQGVFITPG
                     RFSSGAKEYAERDARVRIILVDGAQLAELMIRFGVGVQVKQTHHIVAIDEDFFE"
     gene            1168..2349
                     /locus_tag="PKN52_03660"
     CDS             1168..2349
                     /locus_tag="PKN52_03660"
                     /inference="COORDINATES: protein motif:HMM:NF033543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HNQ99071.1"
                     /translation="MREALIDQPDFFRDIIQVALQRLLEEEITLHLNADPHERTEERR
                     GYRNGYRPRQLKTRVGTLSLLVPMDREGTFKTELFDRYQRSEKALVLSLMEMYLEGVS
                     TRKVKDITEVLCGTSFSKSTVSRLTGQLDADLSAWRERRLGDVAYPYLVVDARYEHVR
                     VDGRVVSQGVLVVKGVREDGQRELLAVDVADSESEATYDALFRKLKERGLHGVQLVTS
                     DDHKGLVAAIQRHFQGAAWQRCQVHFLRNARGKVARKHQAALTSDLKAIYAAPDREWA
                     LEAARGVVERWSASHPAVAEWIETGIEATLACFSFPESHRRRIRSTNGLERFNQELKR
                     RTRVVRIFPNREACLRLVTALCVEQSEEWLSGRKYLDMDLLEAVSASPKPAREEVGLA
                     A"
     gene            2659..>2882
                     /locus_tag="PKN52_03665"
     CDS             2659..>2882
                     /locus_tag="PKN52_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99072.1"
                     /translation="MADAKSSRRKLYGSVISRLNLATSIVGFVNTISSPEPSFDYNIA
                     PLGYRGTRFMGHFSLAPQLDVRVDMSLGFDS"
BASE COUNT          533 a          872 c          942 g          535 t
ORIGIN      
        1 gttatcatcg gcacctgtcg cgaacccgcc aggctagacc cagtggagtg gctgtctcat
       61 tggtgccgcc ggacccgtcg ccaacgcgcc gtgactatat gcttactcga agaagtcctc
      121 gtcgatcgcc acgatgtggt gcgtctgctt gacctgcacg cccaccccga agcggatcat
      181 gagctcggcc agttgagcgc cgtcgacgag gatgatgcgg acgcgcgcgt cgcgctccgc
      241 gtactccttc gcgccgctgc tgaagcggcc gggggtgatg aacacgccct gctccgcctt
      301 accgctcagc gcgccgacga acgcctggac ctccgggcgc tgcacggcgc ggtcctgggc
      361 gtaccgcttg gcctgaacga agaccttgct taggccaagg acgtcttggt cgatgacacc
      421 gtcgatgccg ccgtctccca ctaggttcgt cacggtcgct acacctccgg tgcccccgta
      481 gcccatggag accaagagtt tgactaccgc ccgttcgaag aaggcagcgt cctgatccaa
      541 caggcggctg aggagttcga ccttgacccc ttctcgtatc cggcgaatgc cctcttccac
      601 ctgctcggtg gggtcgagtt cacccgcttg gaggttctcg gcgttcgcct tgttctccga
      661 tgctccccgc ttgatccacc actcatcgcc aggcctcgcg aacggccgaa tatcggcctc
      721 gctaaggtgg gtcgggtgct ctctcaagag gcttcggccc agctcggtgc actggtaatg
      781 cgagcgctgg ggacgagcga gagccccgac gcgtgtcaag taggaaagcg cccagcctac
      841 gcggttctcg tacaccggct cgcccgatgc caatatggca ctacgttgtt gctcggtcag
      901 gcgcgtagcg tctgctaccg acctcgagat ttcccgacgg tgacgcagag cgccatcagc
      961 caagaactgg agcactggga ccatggactg ctcccaagtg ggtagggccg tcacggaccg
     1021 cgccccgtaa gctctgtaaa ttggatggct ctgcgacttt ctcgtgagag aaaggagcca
     1081 tcgctcctcc aaaatgggaa gtgcgaacaa acccaggaac ggagtggcac gatggctcaa
     1141 ctgaaggata gtcctatggc ggcgcgcgtg cgtgaggcgc tgatcgatca acccgacttc
     1201 ttccgcgaca tcatccaggt ggcgctgcag cggctgctgg aggaggagat caccctgcac
     1261 ctgaacgccg acccgcacga gcgcaccgag gagcggcgcg ggtataggaa cggttaccgc
     1321 ccccggcagc tcaagacgcg cgtgggcacg ctctccctgc tggtgccgat ggaccgcgag
     1381 ggcaccttca agacggagct cttcgaccgt tatcagcgca gcgagaaggc gctggtgctg
     1441 tcgctgatgg agatgtacct agagggagtc agcacccgga aggtcaagga catcaccgag
     1501 gtgctgtgcg ggacgagctt cagcaagtcg acggtgagcc gcctgacggg ccagctcgac
     1561 gccgacctga gcgcgtggcg tgagcggcgc ttaggcgacg tcgcgtaccc gtatctggtg
     1621 gtggacgcgc gctacgagca tgtgcgggtt gatggccgcg tggtgagcca gggcgtgctg
     1681 gtggtgaagg gcgtgaggga ggacgggcag cgcgagctgc tggcggtgga cgtggccgac
     1741 agcgaatccg aggcgaccta cgacgcgctc ttccgcaagc tgaaggagcg cggcctgcat
     1801 ggcgtgcagc tcgtgacgag cgatgaccac aagggcctgg tggccgcgat ccagcgccac
     1861 ttccagggcg ctgcttggca gcgttgccag gtgcacttcc ttcgcaacgc gcgcggcaag
     1921 gtggcgagga agcatcaggc ggcgctcacg agcgacctga aggcgatcta cgccgcgccc
     1981 gaccgggagt gggcgctgga ggccgccagg ggcgtggtgg agcgctggag cgcctcgcac
     2041 ccggcggtgg cggagtggat cgaaacaggt atcgaggcca cgctggcgtg cttctcgttc
     2101 ccggagtccc accggcggcg catccgctcg acgaacggct tggagcgctt caatcaggag
     2161 ctgaagcgcc gaacccgggt ggttaggatc ttcccgaacc gggaagcgtg cctgcggctg
     2221 gtgacggcct tgtgcgtgga gcagagcgag gagtggctct cgggccgcaa gtacctcgac
     2281 atggatctgc tcgaggcggt gtcggcttcg ccgaagcccg ccagagagga ggtgggattg
     2341 gcagcctagc gatgactggc cttggaggga tttacagact taaagggact tgactgcttt
     2401 ctagtgctct tgatttcgat tccatgcgcg cctgactgaa cagcgttgcc ggtgtacatg
     2461 ccgcgctgta caaggtcggg atgtccgttg aagtacgcgt tctctactag tccccttgag
     2521 tggcgtgcga tacttgccgt caacatatcc gataggacgc cggacatcgc ggcgggcctt
     2581 agcatgtcgt caagcctcgg aaggccattg ccgattaaca ttgaattgac atcatagaaa
     2641 aagtcatata catcgagcat ggctgatgcg aagtcctcaa ggcgcaaact gtatggcagc
     2701 gtaatatcgc gattaaacct agcaacatca attgttggtt tcgtcaacac aatatcctcc
     2761 ccggagccaa gcttcgacta taatatcgcg cctctgggct accgcggaac gaggttcatg
     2821 gggcacttct ccctagcccc tcagcttgac gttcgggttg acatgagtct cggatttgac
     2881 tc
//
LOCUS       DAOQYE010000161         2880 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_37264_length_2880_cov_2.469735, whole genome shotgun sequence.
ACCESSION   DAOQYE010000161 DAOQYE010000000
VERSION     DAOQYE010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2880
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37264_length_2880_cov_2.469735"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            377..730
                     /locus_tag="PKN52_03670"
     CDS             377..730
                     /locus_tag="PKN52_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99073.1"
                     /translation="MSTDAASAMKSRLRSDLRVAMKDGRADAVRLIRTLVAALDNAEA
                     PPPPGRRSTELDNLETSPVEVERLLLDAAQVRAVLEAEFQERERAAEEMSLLNQPERA
                     GALRAEMLLIGRYLG"
     gene            787..1317
                     /locus_tag="PKN52_03675"
     CDS             787..1317
                     /locus_tag="PKN52_03675"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HNQ99074.1"
                     /translation="MAKLVFGMNQSLDGYVDHTAFGPSPTLFRHFIEQAREQSGAVYG
                     SVMYEIMRYWDDDRPEFDADERAYAEAWRRLPKWVVSRSAKSVGPNARLVEGDLEAAI
                     RRLKAEREGEIEVAGPNLARSLDQLGLIDEYRIYLHPVVLGHGQPLFVGPRPPLRLLS
                     TDVIDGNVVRLVYVPA"
     gene            complement(1357..2052)
                     /locus_tag="PKN52_03680"
     CDS             complement(1357..2052)
                     /locus_tag="PKN52_03680"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HNQ99075.1"
                     /translation="MGKEMDKAAMIFDFDGVIADSETLANAVLAEKLSGSGLPTTLDD
                     ALTRYQGRRWAEVEALIADGIGGTLPESFSDELKRETLMRLATDLVEVPGATAFIRSL
                     TSFRTCIASSSSFERLGVCLEALGLTDDFRGRIFSSEMVQRGKPHPDIFLHAAAQLGV
                     APSRCIVIEDSPGGVQAGVAAGMTVIGLCAASHIRAGHAERLTQTGAAFVARSWSEVS
                     VTVMELVRSGWPR"
     gene            complement(2283..2549)
                     /locus_tag="PKN52_03685"
     CDS             complement(2283..2549)
                     /locus_tag="PKN52_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99076.1"
                     /translation="MDSFARLIPGGTSLDPATVRAEALAARPFLEWASPEEIAAVGRQ
                     VGAERAELWSCEHRREPHHVAGLSPEEVGRRYTLTYQALPGDGD"
BASE COUNT          499 a          895 c          983 g          503 t
ORIGIN      
        1 acgacgaacc cgacggacgc cccactgttc cgtccgcccg tggagccgag cgactagaac
       61 ggtctgcgtt ccatgtgtcg gctgatggtg gacaagatca cgaccgtacc gagggccaag
      121 atcggatccc tagtgggcca cttggatgac gaggacctcg tgcgactgaa cagagcggta
      181 gtagcgttcc tggggctctc ttcgtcccgt aggtctagcc gtagcgagta gtcgaagcgc
      241 cgcattcatg gatagtcgcg ttgcggaagg tcgcaagcgg gcaagaggat gaggcggcct
      301 tgcgggtggc ggtagcgtga agactcgtcg tgacgaccag gaggctgcga gagggtccgg
      361 gtgacaggat gcagccatga gtacggacgc cgcctccgct atgaagtcgc gcctacgttc
      421 cgacctgcgc gttgccatga aggacggtcg ggccgatgcc gtccggctca tcagaacact
      481 cgttgccgcg ctggacaacg ccgaggcgcc accgcctccc ggtagacggt ccacggagct
      541 ggacaacctg gagacgtcgc ccgtcgaagt cgaacgcctc ttgctggacg cggcgcaggt
      601 gcgcgccgtc ctggaggcgg agttccagga gcgcgagaga gcggcggagg agatgagcct
      661 actgaaccag cctgaacggg caggcgccct acgcgccgag atgctcctca tcgggcgcta
      721 cctggggtag gggctctcca tggcggatgc ctttaaagta cctggaacgt aaggagggcg
      781 caccgcatgg ccaagctcgt gttcgggatg aaccaatcgc tggacggcta cgtcgaccat
      841 acggcgttcg gtccaagccc cacgctcttc cgccatttca tcgagcaggc ccgggaacag
      901 tcaggagccg tgtacggcag cgtcatgtac gagatcatgc ggtactggga cgacgaccgc
      961 cccgagttcg acgcggatga acgcgcctat gcggaggcgt ggcgccggct accgaagtgg
     1021 gtcgtgtcgc gctcggcgaa gtcggtcggc cccaacgccc ggctcgtcga gggcgacttg
     1081 gaagctgcga tccgcaggct gaaggccgag cgcgaagggg agatagaggt cgccggcccg
     1141 aacctggcgc gtagcctcga ccaactcggc ttgatagacg agtaccggat ctacctgcac
     1201 ccagtcgtgc tcggtcacgg ccagcctctc ttcgtcggtc cccgtccgcc gttgcgactg
     1261 ctatcgaccg acgtcatcga cgggaacgtg gtcagactgg tgtacgttcc ggcctgatac
     1321 ggggggaggg cagggagcgg aaggcccgct gggcggtcag cggggccacc cagatcgaac
     1381 cagttccatc acggtcaccg atacctcgct ccaagaccgc gcgacgaagg ccgcgccggt
     1441 ctgcgtgagg cgctccgcgt gaccagcgcg aatatgggac gcggcgcata ggccgatcac
     1501 ggtcatcccc gcggcaaccc ccgcctgcac cccgccaggg ctgtcttcga tgacgatgca
     1561 gcgggaggga gcgacaccga gctgggctgc ggcgtggagg aagatgtctg ggtggggctt
     1621 tccccgctgc accatctctg aactgaagat gcgtccgcgg aagtcatccg tgaggcccag
     1681 ggcttccaaa cagaccccaa gacgctcgaa ggaactcgat gacgcgatgc aggtgcggaa
     1741 gctggtcagg cttcggatga acgccgtcgc gcctggcacc tcgaccaggt ctgtcgcgag
     1801 tcgcatcaac gtttcgcgct tgagttcatc ggaaaagctc tcgggaaggg ttccgccgat
     1861 tccgtccgcg atgagggctt ctacttcggc ccaacgtcgg ccttggtagc gggtgagcgc
     1921 atcgtcgagt gtagtaggta ggcccgaccc cgacagcttc tcggccaaga ccgcgttggc
     1981 gagcgtctcg ctatcggcga tgacgccgtc gaaatcgaag atcatggcag ccttgtccat
     2041 ctccttgccc atactccttg cccatactcc ttgcccatcg cctgcgcgat cagaccgagc
     2101 ggaccctgtc gccgatctcc catgagctcc tcgaacttgt cggcacgtgg cagccggccg
     2161 agctaccgcg ccggttcggc atcgcggcgc aggacggtgt ctgcggtgat gtcgccgccc
     2221 cgcttgagga tgcggaagtc cggtgcatca gtttgcgaga acgtgaggcg gaggacgtag
     2281 gcttagtctc cgtcgccggg tagtgcctgg taggtgaggg tgtagcgccg gccgacttct
     2341 tccggactca gccccgcgac gtggtgaggt tcgcgtcgat gctcgcagct ccagagttcc
     2401 gcgcgctcgg ctccgacctg cctgcccacg gcggcgatct cctcgggact cgcccattcg
     2461 agaaacggcc tcgccgcaag agcctcggcc cggaccgtcg cggggtcgag actggtcccg
     2521 ccggggatga gtcgtgcgaa gctgtccatg cgtcttcagt gtgccgtacc gctgctacag
     2581 ctcgcaatac cgcgaggtgc cggtggttcc gcgatgcggg ggtcggcgtt cggtcatctc
     2641 cggtgtttca cgcggcggta cacttcgacc ggacacaagc cggttcctgg tgtgaggcat
     2701 agttttgggt ggtctcgccg ctcagtcatg gcggcgctag cggcgcggga gagtgcgagg
     2761 ctatggcggg aatcagatta gtggatttca tccgccgcta cgatctggac gacaagggcg
     2821 tcgacgcgat caccagcgat gaggctggac gcgtgcggat cgtattcgac ctgttccact
//
LOCUS       DAOQYE010000162         2879 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_37279_length_2879_cov_2.849858, whole genome shotgun sequence.
ACCESSION   DAOQYE010000162 DAOQYE010000000
VERSION     DAOQYE010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2879
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37279_length_2879_cov_2.849858"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2694)
                     /gene="secA"
                     /locus_tag="PKN52_03690"
     CDS             complement(<1..2694)
                     /gene="secA"
                     /locus_tag="PKN52_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HNQ99077.1"
                     /translation="MLGFVQKLFDNNDREVKRFQSEAVNAANALEAKFEAIDNLAEAY
                     AALRRRHLEGGESLDALLPEAFALTRESAKRYLGLRHYDVQFIGGAALHAGRIAEMKT
                     GEGKTLVATLALALNALSAKGTHLVTTNDYLARTGAEWMGPVYRGLGLKVGVIQHDTD
                     GPKRREAYRNDITYITNSELGFDYLRDNMAFRPDQLVLREDTPLNYAIIDEVDSILID
                     EARTPLIISGPAELATDKYYTMAKVATQLEKGEPAEGDKPATGDYSADAKSKDIHLTE
                     AGIAKAEKLIGIDNIFSPENMEIGHMLRQALRAAEHYHLDQEYVKDDGGQIVIVDEFT
                     GRLMPGRRFGEGLHQAIEAKEGVKIERENQTLATITYQNFFKLYGKIAGMTGTAKTEE
                     KEFQEIYSTDVLVIPTNKPVIRKDEEDIVFRTEEGKFASVVEEIAAVHATGQPILVGT
                     VTIDASERLSKLLKRRGIEHEVLNAKYHGREAEIVAQAGRSGAVTISTNMAGRGTDIV
                     LGGNAEWLARQLVEREGFDRYDSDTELFIKAIMMRRPDQARAALQKLEGLTEEIIPRL
                     ERLRDEADADHVKVVGLGGLHIIGTERHESRRIDNQLRGRAGRQGDPGSSRFYVSFED
                     DLMRLFANERVLGMMDRLGMDDSQPIEARMVTGAIERAQKRVEDRNFGIRKQLLEYDN
                     VMSKQREVIYAQRREVLLGNDMSDEVQEMIASYVDAQVQRYLNPQIEPEERDVGALRT
                     SLADAVPVFETVDFEPYRDKQPDDVTDELVEKMEAAYQAREEELGAPLLRELERFIVL
                     QVVDQHWKEHLHNMDVLRQGIGLRGYGQRNPLQEYAFEGYNLFEEMNANIRLNVAKLL
                     FRVQVNVDQRLERRPQRQAPVQYSAAGATGVAAAGAARGAAA"
BASE COUNT          369 a          923 c         1017 g          570 t
ORIGIN      
        1 cgctgcggcg ccgcgcgccg cgccggcggc cgccaccccc gtggcgcccg ccgcgctgta
       61 ctgcactggc gcctgccgct gcggccggcg ctccaggcgc tggtcgacgt tgacctgcac
      121 gcggaacagc agcttggcga cgttgaggcg gatgttggcg ttcatctcct cgaacaggtt
      181 gtagccctcg aaggcgtact cctggagggg gttgcgttgc ccgtagccgc gcaggccgat
      241 gccttggcgc agcacgtcca tgttgtggag gtgctccttc cagtgctggt cgaccacctg
      301 caacacgatg aaccgctcga gctcgcgcag gagcggggcg cccagctcct cctcgcgggc
      361 ctggtacgcc gcctccatct tctcgacgag ttcgtcggtg acgtcgtcgg gttgcttgtc
      421 gcggtacggc tcgaagtcca ccgtctcgaa gacgggcacc gcgtcggcga gggaggtgcg
      481 cagcgccccc acgtcgcgct cctccggctc gatctggggg ttcaggtagc gctgcacctg
      541 cgcgtcgacg tagctggcga tcatctcctg gacctcgtcg gacatgtcgt tgccgaggag
      601 cacctcgcgg cgctgggcgt agatgacctc gcgctgcttg ctcatgacgt tgtcgtactc
      661 gaggagctgc ttgcggatcc cgaagttgcg gtcctcgaca cgcttctggg cacgttcgat
      721 ggcgccggtc accatgcggg cctcgatggg ctgcgagtcg tccatgccga gccggtccat
      781 catgccgagg acccgctcgt tggcgaagag gcgcatgagg tcgtcctcga acgacacgta
      841 gaagcggctg gagccagggt cgccctgccg gccggcgcgg ccgcggagct ggttgtcgat
      901 gcggcgcgac tcgtggcgct cggtgccgat gatgtgcagg ccgccaagac cgacgacctt
      961 gacgtggtcg gcgtcggctt cgtcgcgcag gcgctcgagg cgcgggatga tctcctccgt
     1021 gagcccctcg agcttctgca gggccgcgcg ggcctggtcc ggcctgcgca tcatgatggc
     1081 cttgatgaag agctccgtgt cggagtcgta gcggtcgaag ccctcgcgct cgacgagctg
     1141 acgcgcgagc cactcggcgt tgccacccag cacgatgtcc gtgccacgac cggccatgtt
     1201 ggtgctgatg gtgacggccc ccgaccggcc ggcctgcgcc acgatctcgg cctcgcgtcc
     1261 gtggtacttg gcgttcagga cctcatgctc gatacctcgt cgcttaagga gcttggagag
     1321 ccgctcggag gcgtcgatgg tgacggtgcc tacgaggatc ggctggccgg tggcatgcac
     1381 ggccgcgatc tcttcgacga cgctggcgaa cttgccctcc tccgtgcgga agacgatgtc
     1441 ttcctcgtcc ttgcggatga cgggcttgtt cgtagggatc acgaggacgt cggtagagta
     1501 gatctcctgg aactccttct cctcggtctt ggcagtgccg gtcatgccgg cgatcttgcc
     1561 gtagagcttg aagaagttct ggtaggtgat ggtggcgagc gtctggttct cgcgctcgat
     1621 cttcacgcct tccttcgcct cgatggcctg gtggaggccc tccccgaagc gcctaccggg
     1681 catgaggcgg ccggtgaact cgtccacgat gacgatctgg ccaccgtcgt ccttcacgta
     1741 ctcctgatcg aggtggtagt gctcggccgc gcgtagcgcc tggcgcagca tgtggccgat
     1801 ctccatgttc tccgggctga agatgttgtc gatgccgatg agcttctcgg ccttcgcgat
     1861 gcccgcttcg gtgaggtgga tgtccttcga cttggcgtcg gcggagtagt cgccggtggc
     1921 cggcttgtcg ccttcggctg gctcaccctt ctccagctgc gtggcgacct tggccatcgt
     1981 gtagtacttg tcggtggcga gctcggccgg cccggagatg atgaggggcg tgcgcgcctc
     2041 gtcgatcagg atcgagtcga cctcgtcgat gatcgcgtag ttgaggggcg tgtcctcgcg
     2101 cagcacgagt tggtcggggc ggaacgccat gttgtcgcgg aggtagtcga agccgagttc
     2161 agagttggtg atgtaggtga tgtcgttgcg gtacgcctcg cgccgcttgg ggccgtcggt
     2221 gtcgtgctgg atgacgccga ccttgagccc gaggccgcgg tacacggggc ccatccactc
     2281 ggccccggtc ctggcgaggt agtcgttggt ggtcacgagg tgggtgccct tggcggagag
     2341 ggcgttgagc gccaaggcga gcgtggcgac cagcgtcttg ccttcgcccg tcttcatctc
     2401 ggcgatccgc ccggcgtgca gggccgctcc gccgatgaac tggacgtcgt agtgcctcag
     2461 gccgaggtag cgcttcgccg actcccgcgt gagggcgaac gcctccggta ggagcgcatc
     2521 gaggctctcg ccaccctcga ggtgccggcg gcgcagggcg gcgtaggctt cggccaggtt
     2581 gtcgatggcc tcgaacttgg cctccagcgc gttggccgcg ttgaccgcct ccgactggaa
     2641 gcgcttgacc tcgcggtcgt tgttgtcgaa gagtttctga acgaatccga gcatctcgtt
     2701 atggaccttt ccgcgcttgc cgcccggctc cccggcgtac ggggctcacg ggtgcgtacc
     2761 gcctggcgcg ccgtatggcc ggcttgggct cggccggggc gcgcccgctg cggagtcaac
     2821 agggcactct agcacgctgt caggcgcctc cgccgcagca tatgggacgt gtctgtggg
//
LOCUS       DAOQYE010000163         2877 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_37322_length_2877_cov_3.288448, whole genome shotgun sequence.
ACCESSION   DAOQYE010000163 DAOQYE010000000
VERSION     DAOQYE010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2877
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37322_length_2877_cov_3.288448"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(24..395)
                     /locus_tag="PKN52_03695"
     CDS             complement(24..395)
                     /locus_tag="PKN52_03695"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNQ99078.1"
                     /translation="MVDDSKALFLQIAERVEDSIVDGSLAEDSQAPSTNELAAFYRIN
                     PATAAKGIALLVDKGVLHKRRGIGMFVAPGARRALIEERRAAFASTYLEPLAAEAQKL
                     GLDVDELIQLLKERFSERTKA"
     gene            complement(469..1806)
                     /locus_tag="PKN52_03700"
     CDS             complement(469..1806)
                     /locus_tag="PKN52_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99079.1"
                     /translation="MNLSEHVGKATLRLAVVAAILLPGVGTAAFAQGGQAAGSPTLTA
                     PAALRPIAVPARPEGTVLDLFQYDTAFAKQSTSGSAESVLDDVLIGTRSLRVTTDGDG
                     SQVNLRATNVGPFDLSNSFLRLDLKLHGVAALDYLLIYLSTDGFQTYDAFQVLGGQRP
                     REEAYAQDDEWFTITVNLGATVAGAPASIDLSQVTDIQLSLRDAGVGPVTVWYAALES
                     VPSPARGLVSIVFDDARDGVFYYALPLARRLGIPASIAAIVDLVGQPTFMTVEQLHIA
                     ERFGGWEVIAHHTTPLEAGGFDTLSTADLDFELAGVKRWLLDNGFRRGADVIAYPYGG
                     FDSASLEQTRGYFAAGRTIMRTQGLETFPPADPYRVRAISVVPGDGPAVVKALIDRAA
                     RERAWLVLVFHQVAPSGQGYDTYYPAEDLASIFGYLRTADVDTRLFSDVIFGR"
     gene            complement(1803..2441)
                     /locus_tag="PKN52_03705"
     CDS             complement(1803..2441)
                     /locus_tag="PKN52_03705"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HNQ99080.1"
                     /translation="MGGYPAHKTTREALMHELLAAKPEGLALDELAARLGVTRTAVRQ
                     QVASLERDGLVAPNGLRPSGRRPSRTYGLTELGRESFPRRYDLLSLSMLQALRKSQGD
                     EAAEAVLLAMADDLAERWLAAIAGLEPEARKAAVVEKLSELGYHARLTADGRSVEAIN
                     CVYHRVAKETRAVCRFDERLLSRLLGTEVRLTSCMAEGQRSCVFAQRAEGGR"
     gene            2686..>2877
                     /locus_tag="PKN52_03710"
     CDS             2686..>2877
                     /locus_tag="PKN52_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99081.1"
                     /translation="MTALVALVVTLVAATAAAQDGDLLQSPPPEPYVAASAILPLPDF
                     IPGAGALYIDPADAPVGPWL"
BASE COUNT          435 a          966 c         1002 g          474 t
ORIGIN      
        1 gtgcttcacc tcgatgatgc tcgtcatgcc ttggtccttt cggagaaccg ctccttgagt
       61 agctggatca gctcgtcgac gtcgagtccg agcttttgcg cctcggccgc gaggggctcg
      121 aggtaggtgg acgcgaacgc cgcgcggcgc tcctcgatga gcgcgcgccg ggcgccgggc
      181 gccacgaaca tccctatgcc cctgcgcttg tgcagcaccc ccttgtccac caggagggcg
      241 atgcccttgg cggccgtcgc ggggttgatg cggtagaagg ccgccagctc gttggtcgac
      301 ggcgcctggg agtcctcggc caggctgccg tccacgatcg agtcctcgac gcgttcggca
      361 atctggagga agagggcttt gctgtcgtcg accacggctg gtccttccta gtggttagtt
      421 actccactag gtaaccacct aagtgatagg cctgtcaagt gcgggcggtc accgcccgaa
      481 gatcacgtcg ctgaagagcc gcgtatcgac gtcggcggtg cgcaggtagc cgaagatcga
      541 agcgaggtcc tccgcggggt agtaggtgtc gtaaccctgc ccggaaggcg cgacctgatg
      601 gaacacgagg accagccacg cccgctcgcg cgcggcgcgg tcgatgaggg ctttcacgac
      661 cgcgggtccg tcgccaggga ctacgctgat ggcccgtacg cggtatgggt ccgccggcgg
      721 gaacgtctcg aggccttgcg ttcgcatgat ggtccgtccg gccgcgaagt acccccgcgt
      781 ctgctcgagc gaggcgctgt cgaagccgcc gtaggggtac gcgatcacgt cagcgccgcg
      841 gcggaaaccg ttgtccagca gccatctctt gaccccggcc agctcgaagt cgaggtcggc
      901 ggtagacagg gtgtcgaagc cgcccgcctc taagggggtc gtgtggtggg cgatgacctc
      961 ccagccaccg aagcgctccg cgatgtggag ctgctcgacc gtcatgaacg tcggctggcc
     1021 gaccaggtcg acgatggcgg cgatgctggc cgggatgccg aggcgtctgg ccagagggag
     1081 cgcgtagtag aagaccccgt cgcgcgcgtc gtcgaagacg atcgagacga ggccgcgcgc
     1141 tgggctaggc acggactcga gggcggcgta ccagacggtg accgggccga ccccggcgtc
     1201 gcgcaggctc agctggatgt cggtgacctg gctgagatcg atgctcgcgg gcgcgccggc
     1261 gaccgtggct ccaaggttca ccgtgatggt gaaccactcg tcgtcctgcg cgtacgcctc
     1321 ttcccggggc cgttgccctc cgaggacctg gaaggcgtcg taggtctgga agccgtccgt
     1381 gctcaggtag atgagcaggt agtcgagcgc ggccacgccg tgcagcttga ggtcgagtcg
     1441 caggaaggag ttgcttaggt cgaaggggcc gacgttggtg gctcgcaggt tcacctggct
     1501 gccgtcgccg tcggtcgtga cccggagcga gcgggtgcct atgagcacgt cgtccaggac
     1561 ggactcggcg ctaccggacg tgctctgctt cgcgaaggcg gtgtcgtact ggaagaggtc
     1621 gagcacggtt ccctctggtc gcgcgggcac ggcgatgggc cgcaaggcgg cgggggcggt
     1681 gagggtaggt gacccggcgg cctgcccgcc ttgcgcgaac gccgccgtgc caacgcccgg
     1741 cagcaggatc gcggctacga ccgcgagacg gagagtggcc ttaccgacgt gttccgaaag
     1801 gttcatcgtc ccccctcggc gcgttgggcg aagacgcaac tgcgctggcc ctcggccatg
     1861 caggacgtaa gccgtacttc cgtgccgagc aggcgcgaga gcaggcgctc gtcgaagcgg
     1921 cagacggcgc gcgtctcctt ggcgacccgg tggtagacgc agttgatcgc ctctacggac
     1981 ctaccgtcgg ccgtcaggcg tgcatggtaa ccgagctcgc tcagcttctc gaccacggcc
     2041 gccttgcgag cctccggctc cagacccgcg atggccgcca gccagcgctc ggcgaggtcg
     2101 tcggccatgg cgaggagcac ggcctcggcc gcctcgtcgc cctgcgactt gcgcagcgcc
     2161 tggagcatgc tcagcgacaa caggtcgtag cgccgcggga acgactcgcg ccccaactcg
     2221 gtcagcccgt acgtgcggct gggtcgccgg ccgctcggcc ggaggccgtt gggcgccacg
     2281 agcccgtcgc gctccaggga agcgacctgt tgccgtacgg ccgtcctcgt caccccgagc
     2341 cgagcagcca actcgtccag ggccaagcct tccggcttgg cggcgaggag ctcatgcatg
     2401 agcgcttcgc gcgtcgtctt gtgggcggga taaccgccca tggtggcctg gtcctgcgtc
     2461 tcgcacccac tcccttgaac ggcctggcgt gaaaccgcgg tcggagccac tttacccggt
     2521 cggtcgccgc gcccccggtc aaataatgac aataacggct tgcattattg tcattaagga
     2581 gtatcctgtc cgcgtcggga tgaggcctca cccggtcgag cgaaggaaag gaccatcatg
     2641 catacgaccc taaccaacac gtcacctcgg actcggcggc gctccgtgac cgcccttgtc
     2701 gcactcgtcg tcactctggt cgcggctacg gccgcggccc aggacggcga cctactgcag
     2761 tcgcctcccc cggagccgta cgtcgccgcc tccgcgatcc tccccctgcc ggacttcatc
     2821 ccaggcgccg gcgctctcta catcgacccg gccgacgccc cggtcggtcc gtggctg
//
LOCUS       DAOQYE010000164         2871 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_37442_length_2871_cov_3.800426, whole genome shotgun sequence.
ACCESSION   DAOQYE010000164 DAOQYE010000000
VERSION     DAOQYE010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2871
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37442_length_2871_cov_3.800426"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            259..1062
                     /locus_tag="PKN52_03715"
     CDS             259..1062
                     /locus_tag="PKN52_03715"
                     /inference="COORDINATES: protein motif:HMM:NF021214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HNQ99082.1"
                     /translation="MLFLSWQLEPVLLGMIATLAVAYYLCVGPLRKRLAPKEPYPTRY
                     AIIFGIGLIALFLNEGSPLHDLAERYLLIAHMVQHLLLTYAIAPILLAGTPPWLLRAA
                     LANRGMLPVAKVLLNPITTFATFTLVLSVYHLPPIYDAALANTSLHHAIHVIMLVASL
                     MMWWPMMSPLAELPRPPFLARAVYLFLLPVAQLPIFASVTFSPDVLYAAYANMPTRAF
                     GLSPMEDQQVAGAVMKVGGLIAFGLPFIYTFLQWFWTEDKEHRWGGVKP"
     gene            1059..1694
                     /locus_tag="PKN52_03720"
     CDS             1059..1694
                     /locus_tag="PKN52_03720"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HNQ99083.1"
                     /translation="MSRLPQPLRAIAFDWGGVFTEGTFDSRAHARMAELHGLDAADVL
                     PIYLDLMEGFEVGEFDMPEFHARFQAAVGRASTLDAFREAFLGAVRERRAMYDLLASL
                     PDDVPLALLSNNSPELCDVVRDDTRMRKLMTFVFSNEIGVRKPDERAFAALSSALDLP
                     PNEIVFVDDNRDNIEACERLGFVGLLCDTVTGFAARWRSLFPDLPLPPGFR"
     gene            1691..2095
                     /locus_tag="PKN52_03725"
     CDS             1691..2095
                     /locus_tag="PKN52_03725"
                     /inference="COORDINATES: protein motif:HMM:NF022920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3208 family protein"
                     /protein_id="HNQ99084.1"
                     /translation="MTGAEADDYTERLGNDRDLWEGEATTPRAFKLIQGYIWHPRDQA
                     VDLAAYLPETLGPDVHLLFDAMPAAPFTFFDDGTLSATQVVYQLTVVAIVQPGQVPDA
                     LLTEAAERLGERLETTPQGVGWQLMSDLREVD"
     gene            complement(2220..>2871)
                     /locus_tag="PKN52_03730"
     CDS             complement(2220..>2871)
                     /locus_tag="PKN52_03730"
                     /inference="COORDINATES: protein motif:HMM:NF037170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNQ99085.1"
                     /translation="LRLIGLGATRVEWRYGPDPDYTVLADPDGNYFCVIAAGEVAEDP
                     GLRLAQIRYVPDAAGLPAPDAFADRVVRALLALQERELRAFLPIWRRFRASGAALPNS
                     QNEAYRSNEHLLLHVLQSSGSYLAWVCRQLGLPDPGVRPTPAPADVEAQVDDYVEHLL
                     ERWRAGLKDATLAGTRTTVFTSNWGVPLTISAMLEHAVAHPMRHGFQLEELLARRG"
BASE COUNT          417 a         1060 c          934 g          460 t
ORIGIN      
        1 cgccggcgac gtgcacgcca agcgcgaggc cctcggactg cactgaggcc aagcggatac
       61 cgcggccgta ccgcgggtac cgaaccctgc gagcgagcgc gcaccgccac cgtggcggtg
      121 cgcgctcgtt gtgtggccac cacgcgccag gcgcgctggt cgcactgtgc cacccctctt
      181 tacgcaccgc gtacggaagc cgctccactg agtcgcgctg ggccgtcacc gcgcgccgtt
      241 tcggtttagc atgacggggt gctgttccta tcctggcaac tagaaccggt cctgttgggc
      301 atgatcgcca cgctggcggt cgcctactac ctatgcgtcg gaccgctgcg caagaggctg
      361 gcgcccaagg agccgtaccc cacccgctac gccatcatct tcggcatcgg gctcatcgcc
      421 ctgttcctca acgagggctc gcccctgcac gacctcgcgg aacgctacct gctcatagcg
      481 cacatggtgc agcacctgct gctcacgtac gccatcgcgc ccattctcct ggcgggaacg
      541 ccgccgtggc tcctcagggc cgcgctggcg aaccgcggca tgcttcccgt cgccaaggtg
      601 ctgctgaacc ccatcacgac gttcgcgacc ttcacgctcg tcctctccgt ctaccacctg
      661 ccgcccatct acgacgccgc gctcgccaac acgtccttgc accacgccat ccacgtcatc
      721 atgctcgtcg cctcgctgat gatgtggtgg cccatgatga gcccgctcgc cgagcttccc
      781 cgtccgccgt tcctcgcgcg cgccgtctac ctcttcctcc tccccgtcgc gcagctcccc
      841 atcttcgcca gcgtcacctt ctcccccgac gtcctgtacg cggcctacgc caacatgccg
      901 acccgcgcct tcggcctgtc tccgatggag gaccagcagg tcgccggcgc cgtcatgaag
      961 gtcggcgggc tcatagcctt cggcctgccg ttcatctaca cgttcctgca gtggttctgg
     1021 accgaggaca aggagcaccg ctggggcggg gtcaaaccgt gagccggctc ccgcagccgc
     1081 tccgcgccat cgccttcgac tggggcgggg tgttcacgga aggcacgttc gattcgcggg
     1141 cgcacgcgcg catggccgag ttgcacgggc tcgacgcggc cgacgtgctc cccatctacc
     1201 tcgacctgat ggaagggttc gaggtcggcg agttcgacat gcctgagttc catgcgcgct
     1261 tccaggccgc cgtcgggcgc gcctccaccc tcgacgcgtt cagggaagcg ttcctgggcg
     1321 ccgtgaggga gcgcagggcc atgtacgacc tcctcgcctc cctgccggac gacgtgccgc
     1381 tcgccctgct ctcgaacaac tccccggagc tttgcgacgt cgtgcgcgac gacacccgca
     1441 tgcgcaagct gatgaccttc gtgttctcga acgagatcgg ggtgcgcaag ccggacgaac
     1501 gggcgttcgc ggccctgagc tcggcgctcg acctgccgcc taacgagatc gtcttcgtcg
     1561 acgacaaccg cgacaacata gaggcctgcg agcggcttgg cttcgtcggg ctgctctgcg
     1621 acacggtcac cggcttcgcc gcgcgctggc ggtcgctctt cccggacctg cccctccccc
     1681 cagggttccg atgaccggcg ccgaggccga cgactacacg gagcgcctcg gcaacgaccg
     1741 cgacctctgg gagggcgagg cgacaacgcc ccgcgccttc aagctcatcc agggctacat
     1801 ctggcacccg cgcgaccaag cggttgacct ggccgcctac ctgccggaga ccctcggccc
     1861 ggacgtgcat ctgctcttcg acgccatgcc ggccgctccg ttcacgttct tcgacgacgg
     1921 cacgttgagc gccacgcagg tcgtctacca gctcacggtg gtcgccatcg tccagccggg
     1981 ccaggtgccc gacgcgctcc tgaccgaagc ggcggaacgc ctgggtgaac ggctcgagac
     2041 caccccccag ggcgtcgggt ggcagctcat gagcgacctg cgtgaggtcg actgaacgcg
     2101 taggccggcc cgcgagcggc gggcgcaggg ggtcggtcgg ggtcgatcgg ggtcggtctg
     2161 gcgtcgatcg atggtcggcc gggagctgac cgggggtcta ccgagggtct accagcgcct
     2221 caaccgcgcc tggccagcag ctcctccagc tggaaaccgt gccgcatggg gtgggcgacc
     2281 gcgtgctcga gcatggccga gatggtcagc ggcacgcccc agttggacgt gaaaacggtg
     2341 gtccgcgtgc cggccagggt cgcgtccttc aagcccgcac gccaccgttc gagcaggtgc
     2401 tcgacgtagt cgtcgacctg cgcttcgaca tccgcgggcg cgggcgtagg gcgcacgccc
     2461 ggatcaggca gccccaactg ccggcagacc cacgccaggt agctcccgga ggactgcagg
     2521 acgtgcagca gcaggtgctc gttggaccgg taggcctcgt tctgcgagtt gggtagtgcg
     2581 gcaccgctgg cccggaaccg gcgccagatg ggtaggaagg cccgcagttc acgctcttgc
     2641 aacgcgagga gcgccctgac cacccggtct gcgaaggcgt ccggggccgg gagtccggcg
     2701 gcgtcgggca cgtaacgtat ctgggcgagc cgcaggccgg ggtcctcggc gacctcgccc
     2761 gcggcgatca cgcagaagta gttgccgtcc gggtcggcca gcaccgtgta gtcgggatcc
     2821 ggcccgtagc gccactccac cctggtggcc ccgaggccga tgaggcgcag c
//
LOCUS       DAOQYE010000165         2867 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_37512_length_2867_cov_2.910028, whole genome shotgun sequence.
ACCESSION   DAOQYE010000165 DAOQYE010000000
VERSION     DAOQYE010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2867
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37512_length_2867_cov_2.910028"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="PKN52_03735"
     CDS             complement(<1..276)
                     /locus_tag="PKN52_03735"
                     /inference="COORDINATES: protein motif:HMM:NF013570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HNQ99086.1"
                     /translation="MRLDRYDSYLTTFHTLVTATDTDQHGTWVRLERSAFYPNAGGQP
                     NDVGTLTSAGGVFEVVGVEARADDDVWHLLATPGGLVPGEQVAGRIDW"
     gene            350..1042
                     /gene="folE"
                     /locus_tag="PKN52_03740"
     CDS             350..1042
                     /gene="folE"
                     /locus_tag="PKN52_03740"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HNQ99087.1"
                     /translation="MSTKQGRVHVEVPAQAGTLAHAGTPAHAGAPAHAGAPIEPLEFD
                     EVGDAGLAGIVSELISGLGEDVGREGLVKTPERVSRSMRYLTSGYGLDVREVVNGALF
                     AAEGPEMIVVKGIEFYSLCEHHLLPFFGSVNIGYIPDKKVLGLSKFARVVDVFARRLQ
                     LQERLSAQVADALVDVLQPLGLAVVSEASHLCMMMRGVQKQGSTTVASVMRGVFQEDA
                     RTRQEFLQSIAR"
     gene            complement(1054..1827)
                     /locus_tag="PKN52_03745"
     CDS             complement(1054..1827)
                     /locus_tag="PKN52_03745"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNQ99088.1"
                     /translation="MLLAFDLDKTLLTDDYRLPDETLAAVRAARQRGHIVTVLTGRPL
                     RAAKAFLDLLEIEVPHAVNHGSYVRNGQGEALRRMRLATAQVDALLEEHLDDATVEFS
                     CVVDDVLYVRDPANERWDWVHAESRAITRFQVGLGLAADKVVFHGTGRTPALDGWVAR
                     RHPDLLRYLWGDGYLEIVPQGGDKGSALEYIAGLLGVPRDQVVAFGDGLNDVTMLGWA
                     GHAVAVGPDVHPDALAAADEHVDSPELGGVAAWLERNAL"
     gene            1968..2327
                     /gene="sdhC"
                     /locus_tag="PKN52_03750"
     CDS             1968..2327
                     /gene="sdhC"
                     /locus_tag="PKN52_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HNQ99089.1"
                     /translation="MYRGREGQWAYFLHRITGVALVLYLMLHVFDISLVMYGPDGPFN
                     AFLMFYHQWPFRIGLVLVIAAVVYHALNGLRIILLDFTTWAIRYQRVLWYTVLGVTTI
                     IGIPVLVKILPEIFGGA"
     gene            2327..2755
                     /gene="sdhD"
                     /locus_tag="PKN52_03755"
     CDS             2327..2755
                     /gene="sdhD"
                     /locus_tag="PKN52_03755"
                     /inference="COORDINATES: protein motif:HMM:NF013307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HNQ99090.1"
                     /translation="MAVRPRNLQEARDRSRNNAELAWWVFMRISGLFLVFLVFAHVFI
                     NNIAINVATVDYSYVAGRLAKPSVKVFDTFLLGLAMLHGVNGLRVVIGDNVRRPGLRF
                     WIQVVLIVVSVAIFVAGVMTLWAFSYREMGDAIRDLTSGH"
     gene            2777..>2867
                     /locus_tag="PKN52_03760"
     CDS             2777..>2867
                     /locus_tag="PKN52_03760"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HNQ99091.1"
                     /translation="MIRAHKFDVIVVGAGGAGLMAARYAAHNPK"
BASE COUNT          431 a          948 c          995 g          493 t
ORIGIN      
        1 ccagtcgatc ctaccggcga cctgctcgcc cggtacgagg ccgccggggg tcgcgagcag
       61 gtgccagacg tcgtcgtcgg cgcgcgcctc gacgccgacc acctcgaaga cgccgcccgc
      121 cgaggtgagg gtgccgacgt cgttgggctg accacccgcg ttagggtaga acgcgctgcg
      181 ctccaggcgt acccaggtgc cgtgctggtc ggtgtccgtc gccgtgacga gcgtgtggaa
      241 ggtggtcagg tagctgtcgt agcggtcgag tcgcacgcca gagagtgtag cgccggtcgc
      301 gtcggtgcgg cggacgtccg gtagcatgcc ctcccaagga ggcggtggtg tgagcaccaa
      361 gcaggggcgc gtccacgtcg aggtgccggc acaagctggc acgctggccc acgctgggac
      421 gccggcgcat gccggggcac cggcgcatgc cggggcgcct atcgagccgt tggagttcga
      481 cgaggtaggc gacgccggcc tcgccggcat cgtgtccgag ctgatctcgg ggttgggcga
      541 ggacgttggg cgcgaggggc tcgtgaagac gccggagcgc gtttcccgct ccatgcgcta
      601 cctgacgtcc ggatacgggc tcgacgtgcg cgaggtcgtc aacggcgccc tcttcgcggc
      661 cgagggcccg gagatgatcg tcgtcaaggg catcgagttc tactcgctct gcgagcacca
      721 cctgctcccg ttcttcggca gcgtgaacat cggctacatc cctgacaaga aggtgcttgg
      781 gctatcgaag ttcgcgcgcg tggtggacgt gttcgcgcgc cgcctgcaac tccaggagcg
      841 cctcagcgct caagtggccg acgccctggt ggacgtcctc caaccgctcg ggctcgcggt
      901 cgtgtcggag gcgagccacc tctgcatgat gatgcggggc gtccaaaagc aaggctccac
      961 cacggtggca agcgtgatgc gcggggtctt ccaggaggac gcgcgcacgc gccaggagtt
     1021 cttgcagtcg atcgcgcgct gacggcgcgt cggctaaagc gcgttgcgct cgagccacgc
     1081 ggccacgccg ccgagctcgg gcgagtcgac gtgctcgtcg gcagcggcga gggcgtccgg
     1141 gtggacgtct ggcccgacgg ctacggcgtg accggcccag cccagcatgg tgacgtcgtt
     1201 gaggccgtcg ccgaacgcga ccacctggtc gcgcggcaca ccgaggaggc cggcgatgta
     1261 ctcgagagcg ctgcccttgt cgccgccctg cggaacgatc tccaggtagc cgtcgcccca
     1321 caggtagcgc agcaggtcgg ggtgtcgcct tgccacccag ccgtcgagcg cgggcgtgcg
     1381 gcccgtgccg tggaacacga ccttgtcggc cgccagcccg aggccgacct ggaagcgcgt
     1441 gatggcgcgg ctctccgcat gcacccaatc ccaacgctcg ttggccgggt cgcgtacgta
     1501 cagcacgtcg tcgaccacgc aggagaactc gacggtcgcg tcgtcaaggt gctcttccag
     1561 tagggcgtcg acctgggcgg tggccaggcg catgcggcgc agcgcctcgc cctggccgtt
     1621 tcgcacgtag gagccgtggt tgaccgcatg cggcacctcg atctcgagga ggtcgaggaa
     1681 cgccttggcc gcgcgtagcg gcctgcctgt caggacggtg acgatatgac cgcgctgccg
     1741 cgcggcccgc acggcagcga gcgtttcgtc gggtaagcgg tagtcgtcgg tgagcagggt
     1801 cttgtctaag tcgaaagcta gcagcacaac gtgacgacca taccagtcgg tggcggtcgc
     1861 cgagtgtcgt tcgggcggcc cggcggccag gggccgctgt cgggaccggt gatacaatct
     1921 catgacaatg tcgggtggcg ccaagggcgg cccgcggagg ttgattcatg taccgaggtc
     1981 gcgagggtca gtgggcctac ttcctgcacc ggatcaccgg tgtggcgttg gtcctctacc
     2041 tgatgctgca cgtgttcgac atctcgctcg tcatgtacgg gccggacggc cccttcaacg
     2101 ccttcctgat gttctatcac cagtggccct tcaggatcgg gctcgtgctc gtcatcgcgg
     2161 cggtcgtcta ccacgctctc aacgggttac gcatcatcct cctcgacttc acgacctggg
     2221 ccatccgcta ccagcgcgtg ttgtggtaca cggtcctcgg cgtcacgacc atcatcggca
     2281 tccctgtcct cgtgaagatc ctgccagaga tcttcggggg cgcctgatgg ccgtccgtcc
     2341 aaggaacctg caggaggcgc gcgataggtc gcgcaacaac gcggagctcg cctggtgggt
     2401 gttcatgcgc atctccgggc tgttcctcgt gttcctcgtg ttcgctcacg tcttcatcaa
     2461 caacatcgcc atcaacgtcg ccaccgtcga ctacagctac gtagccggcc gcctcgccaa
     2521 gccgagcgtc aaggtcttcg acacgttcct cctcgggctc gccatgctgc acggcgtaaa
     2581 cggcctccgg gtggtgatcg gcgacaacgt cagacggccc ggcctgcgct tctggatcca
     2641 ggtcgtgctc atcgtggtca gcgtcgccat cttcgtggcc ggggtcatga ccctctgggc
     2701 cttctcgtac cgggagatgg gcgacgccat ccgcgatctc acgagcggcc actagggaag
     2761 ggtgactcaa gcaggcatga tcagggctca caagttcgat gtcatcgttg tcggcgccgg
     2821 cggcgccggc ctgatggccg cacgctacgc ggcgcacaac ccgaagg
//
LOCUS       DAOQYE010000166         2862 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_37608_length_2862_cov_2.705379, whole genome shotgun sequence.
ACCESSION   DAOQYE010000166 DAOQYE010000000
VERSION     DAOQYE010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37608_length_2862_cov_2.705379"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..890)
                     /locus_tag="PKN52_03765"
     CDS             complement(<1..890)
                     /locus_tag="PKN52_03765"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNQ99092.1"
                     /translation="MRRFVLLAIVLLLPAFAAAQGRLTTAIGSNPPTLDPQKTFNGFS
                     FAITNQVYETLFRVTPQGEIEGLLATGWEYLDPSTLRVTLRAGVTFTDGTPLDAAAVK
                     ASLERLLDPATAAPGRFVVSAINEVTAVDATTVEIKTAEPFAPLLAHLAHPVTAIVPV
                     AHGDDLARNPVGSGPYTLVSWEQSNQVLLRANADYWGGAPAIEEVLYRIIPEVSTQLI
                     ELRSGGIDILFNIPPDAFETLASESQLETQSYLGWGSMMLGLNTTNPKLRDLRVRQAI
                     AHAIDKGLIIDEFLRGLAEPG"
     gene            1117..>2862
                     /locus_tag="PKN52_03770"
     CDS             1117..>2862
                     /locus_tag="PKN52_03770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNQ99093.1"
                     /translation="MQEEPTVFVRGEAAPERLAVEAWKERTREPRLALSLALDALDGG
                     AQGMAGGLALAARAVASLALDEPAEALAAAKRALVTLRGAPAPAGDRRGSSEEWPVES
                     EETLDEVTVDVLLSALRAAFLLEDPVGAARYGQDALALAEAKGFTSQVARVHSDLAAV
                     YGSRDVLDVALRHLTAGVAALEQAGLPVAPALLNNLGNVYLDTSRVDEALACFTRARE
                     TFQDTGDPFGAALARSNEGRAYMRLGRFALAVEALEEALAALNKAGNRTYGAATLAKI
                     AQAHAAAGDERQAAAKFEEAIKALRPDHDPWEGEVRAAFGEHLLSAGRASDALRELER
                     AERLFRTAGTPLAATDLKRQQAAALANLGRFKEAYERLADYLSERERGSADRNELLLR
                     LLLVEFEAGLGAQHELHVVARQAIVEANRSLREQAALLEGLSITDELTELFNRRYFRR
                     RLQEELVRARRDGSEVVVLLVDVDHFKHVNDSYSHTVGDAVLTTIAGILRRNCRASDV
                     VARWGGEEFAVLMPGTTRATAFEVAERARRAVAEHSWRDLAPGVAPGLALTVSIGVAS
                     TSELQTLASGDPEAQL"
BASE COUNT          422 a          907 c         1086 g          447 t
ORIGIN      
        1 cccggctcgg cgaggccgcg caggaactcg tcgatgatga ggcccttgtc gatggcgtgg
       61 gcgatggcct ggcgcacgcg caggtcacgc agcttggggt tggtcgtgtt gagaccgagc
      121 atcatgctgc cccagccaag gtacgactgc gtctcgagct gcgactcgct ggcgagcgtc
      181 tcgaaggcgt ccggcgggat gttgaagagg atgtcgatgc cgccggaacg aagctcgatg
      241 agctgcgtgc tcacctcggg gatgatccgg tacaggactt cctcgatggc aggcgcgccg
      301 ccccagtagt cggcgttggc gcgcaggagc acctggttgc tctgctccca cgacacgagg
      361 gtgtacgggc cggagccgac ggggttgcgg gcgaggtcgt cgccgtgcgc cacgggcacg
      421 atggcggtga cggggtgcgc gaggtgggcg aggaggggcg cgaacggctc ggccgtcttg
      481 atctcgaccg tggtcgcgtc gacggccgtc acctcgttga tggcgctgac cacgaagcgg
      541 cccggcgcgg ccgtggcggg gtcgaggagg cgttccagcg acgccttgac ggcggcggcg
      601 tcgaggggcg tgccgtcggt gaaggtcacg ccggcgcgga gggtgacgcg cagcgtgctt
      661 gggtcgaggt actcccagcc ggtggcgagg aggccctcga tctcaccctg gggagtgacc
      721 ctgaagaggg tttcgtaaac ctggttggtg atggcgaacg agaagccgtt gaacgtcttc
      781 tgcgggtcga gcgtcggcgg gttggagccg atggcggtgg tgaggcgccc ctgcgccgcg
      841 gcgaacgcgg gtaggagtag gactatggcc agcaggacga atcttctcat gcaccctccc
      901 aggtggtggc ggccgaaggt cggggtcggt cttcccgtac cgcggcggcg caatcgatgc
      961 taagggatga cggcgcccat gcggagtccg ctgggttgca tgaagccttg ctaaaggtcg
     1021 tcggtcgctc ccatatactg tcgcccaacc ccgcgcggca gggggtcggc gtcgcccggc
     1081 accttccaag cgtgcgcggc agggcccggt gagtggatgc aggaagaacc gacagttttc
     1141 gttcgtggcg aggccgctcc ggaacgcctt gcggtggagg cttggaagga gcgcacgcgc
     1201 gaaccgcgcc ttgcgctcag cctcgcgctt gacgcgctgg acggcggtgc gcaggggatg
     1261 gccggcggcc ttgcgttggc cgctcgcgct gtcgccagcc tggcgctcga tgagcctgcc
     1321 gaggcgttgg cggcggcaaa gcgcgcactg gtgaccctgc gaggcgcgcc ggcgccggcg
     1381 ggcgatcggc gtggatcgtc ggaggagtgg ccggtcgaga gcgaggagac gctcgatgag
     1441 gtgacggtcg atgtcctctt gagcgcgctg agggcggcct tcctgctgga ggatccggtc
     1501 ggcgcggcgc gttacggtca ggacgcgttg gctcttgccg aggccaaggg gttcacgagc
     1561 caggtcgcgc gcgtccatag cgacctggcg gccgtctacg gctcacgcga tgtcctcgac
     1621 gtcgccctgc gccatctgac cgccggtgtc gccgcgctcg agcaggcggg tcttcctgtc
     1681 gccccggctc tcctcaacaa ccttggcaac gtctacctcg atacgagcag ggtggacgaa
     1741 gcgttggcat gcttcacgcg tgctcgcgag acgttccagg atacgggcga tccgttcggg
     1801 gccgcgctcg cccgctccaa cgagggcagg gcctacatgc gcctcgggcg cttcgccctc
     1861 gccgtcgagg cgttggagga ggcgctcgcg gcgctgaaca aggcggggaa ccgcacgtac
     1921 ggcgcagcca cgctcgccaa gatcgcgcag gcgcacgccg cggcgggcga cgagcgccag
     1981 gcggccgcca agttcgagga ggcgatcaag gcgctacgcc cggaccacga cccgtgggag
     2041 ggggaagtgc gagccgcgtt cggcgagcat ctgctgagcg ccgggcgcgc ctccgatgcc
     2101 ctgagggagc tggagcgcgc cgaacgcctg ttccgaactg caggcacccc gctggcggct
     2161 acagatctca agcggcagca ggcggcggcc cttgccaacc tggggcggtt caaggaggct
     2221 tacgagcgcc tggccgacta cctgtccgag cgcgagcgcg gctctgccga tcggaacgaa
     2281 ctgctcctgc ggctcctcct cgtcgagttc gaggcgggcc tcggcgcgca acacgagctc
     2341 cacgtggtgg cacggcaggc gatcgtcgag gccaacagga gcctgcggga gcaagctgcc
     2401 cttctcgagg gtctttccat caccgacgag ctgacggaac tcttcaatcg ccggtacttc
     2461 aggcggcgcc tccaggaaga gctggtacgc gctcgtcgcg acggctcgga ggtagtggtc
     2521 ctcctcgtcg acgtcgacca cttcaagcat gtgaacgaca gctactcgca caccgttggc
     2581 gacgccgttc tgacgaccat cgccggcatc ctgcggagga actgtcgggc ttccgacgtc
     2641 gtggcgcggt ggggtggcga ggagttcgcc gtcctgatgc cgggaacgac gagggcgacg
     2701 gcgttcgagg tcgcggagcg cgccaggcgc gccgtcgcgg agcactcatg gcgtgacctc
     2761 gccccgggcg tggcacccgg actggcgctc acggtcagca tcggcgtcgc ctctaccagc
     2821 gaactccaga cgctcgcgag cggtgatccg gaggcgcagc tt
//
LOCUS       DAOQYE010000167         2853 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_37803_length_2853_cov_2.853109, whole genome shotgun sequence.
ACCESSION   DAOQYE010000167 DAOQYE010000000
VERSION     DAOQYE010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2853
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37803_length_2853_cov_2.853109"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="PKN52_03775"
     CDS             <1..367
                     /locus_tag="PKN52_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HNQ99094.1"
                     /translation="GTGAGLNYGWKVMEGLHCYEASDCDQAGLTLPVIEYPHGPQWGT
                     SITGGYVYRGSDVPALQGSYVFADFVSGRVWSASAEGGWAPKPLFETGFNVSTFGEDA
                     AGELYVAAYDSGMIYRVGR"
     gene            complement(474..1397)
                     /locus_tag="PKN52_03780"
     CDS             complement(474..1397)
                     /locus_tag="PKN52_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HNQ99095.1"
                     /translation="MNALRNETLTAVAGLRVGHHTDLEARTGCTVVLTPEGGCVASGV
                     ALGPAPGSREYVLLQPDKTVQTVDAVVLTGGSAFGLASADGVMLWLEEQGRGFDTGVA
                     RVPIVPAAVLFDLAVGSAKVRPDAAAGRAAAVAATDAPVQQGRVGAGTGATVGKLRGP
                     DQATDSGLGSHAVQVGGAVIAAIGISNAAGNVVDPDTGKLVAGVPETLGLAAATGFAA
                     MPGSNTTLVLVATDAPLTKAQANALATSAHIGIARVTRPSHTVFDGDSAFVLSTGTGP
                     EVPLAALSVAVQEVVAAALVKGVLAGREAGA"
     gene            complement(1397..>2853)
                     /locus_tag="PKN52_03785"
     CDS             complement(1397..>2853)
                     /locus_tag="PKN52_03785"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNQ99096.1"
                     /translation="ADRTWRYPLEPLGWDVAYDGQGNIVTAVRTGAEVVVQSSVSSDL
                     SRDVLIAVRNWLTQRYGESVVFGIGGLRVTTTIDVQAQVAANQASLNGEVPPGAQLAV
                     VGIDPSTGAVLAMVGQRLVEGVPPGEFNRATMALRQPGSSFKPIVYATAIEQGAMNQS
                     TILVDEPTKFLIRGQPAYEPGNHDHTYDGFQTIRASLNRSRNIPAVKALEAAGVDAVA
                     EKARELGYDVKPYYAMALGSFEVTPLQHTAAMAAFANGGVYIEPYFIERVEDADGNVL
                     YQAEPRSARVWSEQTAYIMLDMMHGNVADREPAYGLSNRASVPGRWIAGKTGTTNDEN
                     DIWFVGMTPGMVAAVWIGNDLPTSLPSRMTLSNGNTDQVNSSRQPIYVWNDFVTAALR
                     GSPATGEGFPVPEGIVFKNMDLKTGAVGSGGVRAAFRATDTAAGGGLDAAIKLSLPID
                     TATGKRATVDTPPDRIEIIEVDPRDVGNYLEGTG"
BASE COUNT          392 a          963 c         1032 g          466 t
ORIGIN      
        1 gggcacgggc gccgggctca actacggttg gaaggtcatg gagggcctgc actgctacga
       61 ggcctccgac tgcgaccagg ccgggcttac gctcccggtg atcgagtacc cgcacggtcc
      121 gcagtggggc acgtcgatca cgggcggtta cgtgtatcgc gggtcggacg ttcccgctct
      181 gcaaggtagc tacgtgttcg cagacttcgt gtccggccgc gtttggagcg ccagcgcgga
      241 gggcggctgg gcccccaaac cgctcttcga gacgggcttc aacgtgagca cgttcggtga
      301 ggacgccgcc ggggagctgt acgtggcggc ttacgacagc ggcatgatct accgcgtcgg
      361 tcgttgagcc gcccccagcg gccgtcatcc gaaacgtcgc gggcgccggg cctaaaagcc
      421 cggcgcccgt tcgttcgtac gtgggcgtga cgacgcccaa gacacccgac cgactaggcg
      481 ccggcctctc gaccggcgag cacgcccttg acgagcgccg cggccacgac ttcctgaacg
      541 gcgacggaga gggccgcgag aggcacttcc ggacccgttc cggtcgagag cacgaaggcg
      601 ctgtcgccat cgaagaccgt gtgtgatggg cgggtgaccc tcgcgatgcc gatgtgggcg
      661 ctcgtcgcga gcgcgttggc ctgagctttc gtgaggggcg catccgtggc gacgagcacc
      721 aacgtcgtgt tgctgccagg catggcggcg aaaccggtgg cggcggcgag gccgagcgtc
      781 tccggcacgc cggcgacgag cttgcccgtg tcggggtcca ccacgttgcc tgcggcgttg
      841 ctgatgccga tcgccgcgat gaccgcgccg cccacctgca cggcgtggct gcccaggccg
      901 ctgtctgtgg cttggtcggg gccgcgcagc ttgccgacgg tcgcgccagt cccggcgcct
      961 acccggccct gctggactgg tgcgtcggtg gcggcgacgg cggcggctcg accggcggcg
     1021 gcgtcgggcc gcaccttcgc cgaaccgacc gcgaggtcga acagcaccgc cgcgggcacg
     1081 atgggcacgc gcgccacacc cgtgtcgaag ccgcgacctt gctcctccag ccagagcatg
     1141 acgccgtcgg ccgaggccag cccgaatgcg ctgccacccg tgaggacgac cgcgtcgacc
     1201 gtctggaccg tcttgtcggg ctgcaggagg acgtactcgc gcgagccggg ggccggacca
     1261 agcgctactc cggaggcgac gcagccgcct tccggcgtga gcacgacggt gcaaccggta
     1321 cgggcctcga gatcggtgtg atggccgacc ctgaggccgg ccacggcggt gagggtctcg
     1381 ttccggagcg cgttcatcag ccggtccctt cgaggtagtt ccccacgtcg cgcgggtcga
     1441 cctcgatgat ctcgatgcgg tcgggcggcg tgtcgaccgt cgcccgcttc ccggtggcgg
     1501 tgtctatcgg aaggctcagc ttgatggcgg cgtccaggcc gccacccgcg gccgtgtcgg
     1561 tcgcgcggaa cgcagcccgt accccgccgc tgccgacggc tcccgtcttg aggtccatgt
     1621 tcttgaaaac gatgccttcc gggaccggga agccctcgcc ggtggccggg ctccccctga
     1681 gcgccgcggt gacgaagtcg ttccagacgt agatgggttg gcgtgagctg ttgacttggt
     1741 cggtgttgcc gttgctgagg gtcatacggc tcggcaggct cgtcgggagg tcgttgccga
     1801 tccacacagc ggcgaccatg ccgggcgtca tgccgacgaa ccagatgtcg ttctcatcgt
     1861 tggtcgtgcc ggtcttgccc gcgatccacc gacccgggac ggacgcgcgg ttcgagaggc
     1921 cgtaggcggg ttcgcggtcc gccacgttgc cgtgcatcat gtcgagcatg atgtaggccg
     1981 tctgctcgct ccacacgcgc gccgaacgcg gctccgcctg atacaggacg ttgccgtcgg
     2041 catcctcgac gcgctcgatg aagtacggct ctatgtacac gccgccgttg gcgaaggcgg
     2101 ccatggccgc ggtgtgctgc aggggcgtga cctcgaagga gccgagcgcc atggcgtagt
     2161 agggcttcac gtcgtagccg agctcgcgcg ccttctccgc caccgcgtcg acaccggccg
     2221 cctccagggc cttgacagca gggatgttgc ggctccggtt caggctcgcc ctgatcgtct
     2281 ggaagccgtc gtaggtgtgg tcgtggttgc ccggctcgta cgccggctgg ccgcgtatga
     2341 ggaacttcgt cggctcgtca accaggatgg tggactggtt catggcgccc tgctcgatgg
     2401 cggtggcgta cacgatgggc ttgaacgagc tgcccggttg cctaagggcc atcgtggcgc
     2461 ggttgaactc gccggggggc acgccctcga cgagcctctg cccgaccatg gcgaggacgg
     2521 cgcccgtgct cgggtcgatg ccgacgacgg ccagctgggc tccaggcggc acctcgccgt
     2581 tcaggctggc ctggttggcg gccacctgcg cctgcacgtc gatggtggtc gtgaccctga
     2641 ggccgccgat gccgaacacg acgctctcgc cgtagcgctg ggtgagccag ttgcgcacgg
     2701 cgatcagcac gtcgcgggag aggtccgagc tcacgctcga ctggacgacg acctccgcac
     2761 cggtcctcac ggccgtgacg atgttgcctt gaccgtcgta ggcgacgtcc cacccaagcg
     2821 gctcgagagg gtagcgccag gtgcggtcgg ccg
//
LOCUS       DAOQYE010000168         2833 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_38185_length_2833_cov_4.535637, whole genome shotgun sequence.
ACCESSION   DAOQYE010000168 DAOQYE010000000
VERSION     DAOQYE010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2833
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38185_length_2833_cov_4.535637"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..640)
                     /locus_tag="PKN52_03790"
     CDS             complement(<1..640)
                     /locus_tag="PKN52_03790"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HNQ99097.1"
                     /translation="MKKSELPSLARTTLALVLVLATVGLGSAQKYADAAALTAAIDAR
                     PEPATSQATLTMTITTADGQTLTREMQTWSEGTAKRIIKFTAPADIKGSGFLTITAAD
                     GSEEKLVYLPALDRVRRIAGGQQGDSFFGSDFTYEDITGLDQADYTHELLGVRDGPTY
                     VVEAVPTPASGSSYERLVIEVPEATLVPQRVEYYRAGELVKVLTVAGLAEVAG"
     gene            complement(627..>2833)
                     /locus_tag="PKN52_03795"
     CDS             complement(627..>2833)
                     /locus_tag="PKN52_03795"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HNQ99098.1"
                     /translation="LAISATRIETAGATESLYPDDSRVDAIAEELQATFRHHDRLLLV
                     TSGDIYSGPTMARVAALNAALQRLAGVEDVTSVATAKRMVDDDGFLIVDDLIPRHAPG
                     VEPDAEELAAARAYLAGSPMYQNVTLVSSDGRHASFIIEMADGLDANAFAKSVVETVR
                     TEWVGEYALAGQAFTSMELQSIIGRDLPVLGGLALVVIVLMLFLNFRTLQGTLLPLAL
                     ILLGVVWGMGLFGLLGGKLMALTVIGPIAVMAVASSFIINMLGRYYFELAHGAGKRVA
                     IDRMLGETGLGVIVSGVAISAAMSTFMLSSLQMVRGLGLVALLGVLASLLASMVLLPA
                     LLNVLPVPKHVGDPENPGGLARVLASLGRLTTRRPRVVVLTAVAVVLAGLVGVPRIVT
                     DTSILAFFPEGGATRSSVATVEEVLGGSSTITVWLQGDLGDPALLAAMEAYQHDVANL
                     PELGAGQSIVNVIRALNLTLTGEDALPRTREAVAQELLLYQSSGDVSDLTRFMTLDQQ
                     QGVINFVAKSMSTERVAAVTNEMTRLAEARFGDLATVRFTGNPLLEREIEQAMRHDFV
                     ISLTLAIALVLIIDSFVRSFRAAAVTIIVLLSTVALQYGLLGWLGLPLNLATMLMGAL
                     AIGVGDYAIHLTVRYMEERRHGRDPEQAVMHTLYSTGRQILFTALTLGAGFGALAFAS
                     FVPVATLGRLMLLTVGLVGVATLTLLPAASLRFLRDPHARVHLSKELTHEEV"
BASE COUNT          478 a          888 c         1037 g          430 t
ORIGIN      
        1 acccggccac ctcggcgagg ccagcgacgg tgaggacctt cacgagctcg cccgcccggt
       61 agtactcgac gcgttgcggc accagcgtgg cctcgggcac ctcgatgacg aggcgctcgt
      121 agctcgaacc gctcgcgggt gtcgggacgg cctcgaccac gtacgtcgga ccgtccctca
      181 ccccgagcag ctcgtgggtg tagtcggcct ggtcgaggcc agtgatgtcc tcgtaggtga
      241 agtcggaacc gaagaacgag tcgccctgct gcccgccggc gatgcgccgc acgcgatcga
      301 gcgcgggtag gtagacgagc ttctcctcgc tgccgtcggc ggccgtgatg gtcaggaagc
      361 ccgagccctt gatgtctgcg ggcgccgtga acttgatgat gcgcttagcg gtgccctcac
      421 tccacgtctg catctcacgc gtgagcgtct ggccgtcggc ggtcgtgatg gtcatggtga
      481 gggtggcctg gctggtggcg ggctcgggac gggcatcgat ggcggccgtg agggcagcgg
      541 cgtccgcgta cttctgggcg gagccgaggc cgacggtggc gagtaccaag accagcgcga
      601 gggtggtccg cgcgaggctg gggagttcag acttcttcat gggtcaactc cttggagagg
      661 tggacgcgag cgtgagggtc gcgcaggaac ctgaggctgg cggcaggcag gagggtgagg
      721 gtggccactc cgacgaggcc tacggtgagc agcatgagcc ggccgagggt agcgacaggg
      781 acgaagctgg cgaaggctag agcgccgaag ccggcgccga gcgtgagggc cgtgaagagg
      841 atctgcctgc ccgtgctgta gagggtgtgc atgacggcct gctccgggtc gcggccgtgc
      901 cggcgctcct ccatgtagcg cacggtgagg tggatggcgt agtcgccaac accgatggcg
      961 agggcgccca tgagcatggt ggcgaggttg aggggcagcc cgagccagcc gaggaggccg
     1021 tactgcaacg ccacggtgga gaggagcacg atgatcgtca ccgcagccgc acggaacgac
     1081 cgcacgaagc tatcgatgat cagcaccagc gcgatggcga gggtgaggct gatcacgaaa
     1141 tcgtggcgca tggcctgctc gatctcgcgc tcgagcagag ggttgccggt gaatctaacg
     1201 gtcgcgagat cgccgaagcg cgcttcggcc agacgagtca tctcgttcgt cactgccgcg
     1261 acgcgctcgg tcgacatcga cttggccacg aagttgatga cgccctgctg ctgatcgagg
     1321 gtcatgaagc gcgtcaggtc actcacgtca ccagaggact ggtagagcag tagttcctgc
     1381 gccaccgcct cgcgcgtgcg gggcaacgcg tcctcgccgg tgagcgtgag gttgagggcg
     1441 cggatcacgt tcacgatcga ctgcccggcc cccagttccg ggaggttcgc cacgtcgtgc
     1501 tggtaggcct ccatggctgc caggagcgcc gggtcaccga ggtcgccctg caaccacacc
     1561 gtgatggtag aggagccccc cagcacttcc tcgacggtgg ccacgctgct gcgcgtggcg
     1621 cccccttcgg ggaagaacgc caggatggag gtatcggtga ctatgcgtgg gacgccgacc
     1681 aggcctgcca ggactactgc gaccgcggtg aggacgacga cgcggggccg gcgggtggtc
     1741 agccgcccga ggctggcgag caccctggcg aggccacccg ggttctccgg gtcgccaacg
     1801 tgcttcggga ccggaagcac gttgaggagg gccggcagaa ggaccatgct ggccaggagc
     1861 gacgccagga cgcccagcag cgccaccaga ccgaggccgc gcaccatctg gaggctggag
     1921 agcatgaagg tgctcatggc ggccgagatc gcgacgccgg acacgatcac cccgaggcct
     1981 gtctcgccga gcatcctgtc gatggcgacc ctcttccctg ccccgtgcgc cagctcgaag
     2041 tagtagcggc ccagcatgtt gatgatgaac gagctcgcca cggccatgac ggcgatgggt
     2101 ccgatgaccg tcagtgccat gagcttgccg cccaagagcc cgaagaggcc catgccccac
     2161 accacgccca ggaggatcaa cgcgagcggc agtagtgtgc cctgcagcgt gcggaagttc
     2221 aggaagagca tcagcacgat gacgacgagt gccaggccgc ccagcacggg caggtcgcga
     2281 ccgatgatcg actgcagctc catgctcgtg aacgcctggc cggcgagcgc gtactcgccc
     2341 acccattcgg tccggaccgt ctcgacgacg ctcttcgcga acgcgttggc gtccaggcca
     2401 tcggccatct cgatgatgaa gctggcatgg cgcccatcgc tgctgacgag ggtgacgttc
     2461 tgatacatgg gcgacccggc gaggtaggca cgggcggctg ccagctcctc cgcgtcgggc
     2521 tccacgccag gggcatgcct gggtatgagg tcgtcgacga tcaggaagcc gtcgtcgtcg
     2581 accatgcgct tggccgttgc cacggaggtg acgtcctcga cgcccgccag gcgttgcagg
     2641 gccgcgttga gggcagcgac gcgcgccatg gttgggccgg agtagatgtc gccactcgtg
     2701 acgagcagca gacggtcatg gtgccggaac gtggcctgga gttcctcggc gatggcgtcg
     2761 acgcgcgagt cgtccggata gagggactcc gtcgcgccgg ccgtctcgat ccgggtggcg
     2821 ctgatggcca gga
//
LOCUS       DAOQYE010000169         2787 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_39214_length_2787_cov_2.736457, whole genome shotgun sequence.
ACCESSION   DAOQYE010000169 DAOQYE010000000
VERSION     DAOQYE010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2787
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39214_length_2787_cov_2.736457"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            255..902
                     /locus_tag="PKN52_03800"
     CDS             255..902
                     /locus_tag="PKN52_03800"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HNQ99099.1"
                     /translation="MGKIDKAVQALIDELGVDRFYRRILTAMQEGKLGFNLAPTEQIA
                     FVAQPHGVETTLVGRWGLEPGWVREPGQVKGTLFNARAESVAEKPSFRDALRRARCIV
                     PDSGLYEWQVIPGAKRKQPYWITRSDGRPLLFAGLYALHEWGASFTILTTAPNELMQP
                     IHDRMPVILDPADVDRWLDSELTDSRAVEDLMRACPPEWLTAKALTRPVGEWEPV"
     gene            1055..>2787
                     /locus_tag="PKN52_03805"
     CDS             1055..>2787
                     /locus_tag="PKN52_03805"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNQ99100.1"
                     /translation="MRLTLLARPVVETSGRAELLHAHKPVLLLVYLACRGDWVSRDEL
                     TGLFWPDSDETGARHNLRSLLHRARRLPWANGLEVETDWLRWQVSSDLSDFKAAISAG
                     NWAKACELHQQQLLGGFLVEDAQGLEEWLNLERESLQGAWRDACLRQARDLRAASRHA
                     EAAELLARYLSHDELAEDVLVDFMESSAVAGRRERALSAYQRFEANLNAELGLDPLPE
                     TRKLATAIGSGLSVVGQTPDHSVWSLPPLLERPVRLVGRSSELAALRDSSARLKLVSG
                     EAGIGKSRLLLEAFPDAILLSSREGLVDSPFYPIVDLIRARLTELPNLGPYLEDLARL
                     VPEVAPEAKPVPVDARLGAARLLEALARLLEGLGSTLVFDDLQWADSGTHEVLALLCA
                     RAGVDCVGAYRVGEASAELRDLAASLSRRGELLELHLPGLRQVDVDDLLAAMSGADGH
                     WPRLSAWLFARSAGNPLFALETIKALFDTGVFLADDSDWPAKLEGLVDGLEVSALPAA
                     VTELIQRRVSALSGGARRLLQVTSVLGGDASPELLTSLVGLSDWGLLDAIEEAEAHGL
                     LAGASFVHDVQS"
BASE COUNT          447 a          922 c          959 g          459 t
ORIGIN      
        1 gctggctgat catggcccag ccggtcatca gcgggatcat gacgaccgcc ccgccgagcc
       61 cgaccatgct cccgaagaaa ccgcccgcca gaccgatgat gagagcttgc catgagagag
      121 acatcacgcg aaaccttaca cccatcgggg cgcgggtgcg gcggggcgct tccggaggac
      181 atgccccctt gtggcctctc gtcagggtac cgggccgatc ccgcgctacc atcgttagtg
      241 gcatgtgcgg acggatgggc aagatcgaca aggcggtgca ggctctgatc gacgagctgg
      301 gcgtcgaccg cttctaccgc cgcatcctga ctgccatgca agagggcaag ctcggcttca
      361 acctcgcccc taccgagcag atcgcgttcg tggcgcagcc gcacggcgtg gagacgacgc
      421 tcgtcggacg ttggggccta gagcctggct gggtgcgcga acccgggcag gtcaagggca
      481 cgctcttcaa cgcgagggcc gagtccgtcg cggagaagcc ttccttcagg gacgcgctgc
      541 ggcgtgccag gtgcatcgtg ccggacagcg gcttatacga gtggcaggtc atccccggcg
      601 ccaagcgcaa gcagccctac tggatcaccc ggtccgacgg gcgccccctg ctcttcgccg
      661 gcctctacgc actacacgaa tggggagcgt cgttcaccat cctgacgacc gccccgaacg
      721 agctcatgca gccgatccac gatcgcatgc ccgtcatcct ggaccccgcc gacgtagacc
      781 gttggctgga ctccgagttg accgactccc gggccgtcga ggacctcatg cgggcttgtc
      841 caccggagtg gctgacggcg aaggcgctga ccaggccggt gggcgagtgg gagccggtgt
      901 gacggggcgt agtgaaggcc caggactacg ccaggacgag caggtcgggc gcccggtcgc
      961 ggagcggtcg aaggccgaat agcgggtctg ccaagctaga cgaggcacaa gttgccggca
     1021 ctgggccggc gacgacgttt atcatccttc tctagtgcgc ttgacgttgt tggccaggcc
     1081 agtcgtggag acgagcgggc gtgctgagtt gcttcacgct cacaagccgg tactgctgct
     1141 cgtctatctc gcctgccgcg gcgactgggt aagtcgcgac gagcttaccg ggctgttctg
     1201 gcccgatagc gacgagaccg gcgcccgaca caacctccgt tcactgctgc acagggcgcg
     1261 gcgactgcca tgggccaacg gcctggaagt ggagaccgat tggttacgct ggcaggtctc
     1321 cagcgacctg tccgacttca aggcggccat ctcggcgggc aactgggcca aagcctgtga
     1381 gctgcaccag cagcagctcc tcggcggctt cctggtggag gacgcccagg gcctcgagga
     1441 gtggctgaac ctcgagaggg agagcctgca gggtgcctgg cgcgacgcct gtctgagaca
     1501 ggcgcgagac ctgcgggcgg cctcgagaca tgccgaggcg gccgagctcc tggcgcgcta
     1561 cttgagccac gacgagttgg ccgaggacgt tctggtggac ttcatggagt cgagcgcggt
     1621 cgcgggcagg cgcgaacgcg cgctgagcgc ctaccagcgt ttcgaagcca acctcaacgc
     1681 ggagctcgga ttggatccgc tgcccgagac gaggaaactg gcaacggcca tcggcagcgg
     1741 tctaagcgtg gtcggtcaga cacccgacca ctccgtctgg tcactacctc ccctgctcga
     1801 gagacccgtc aggctcgtcg gacgctcgag cgagttggcc gcactgcgtg acagcagcgc
     1861 gaggctgaag ctggtctccg gcgaggccgg catcggcaag agccgattgc tgctcgaggc
     1921 cttccccgac gccatcctcc tctcctcgcg ggaagggctg gtcgactcgc ccttctatcc
     1981 gatcgtcgac ctgatccgtg cgcgcctgac cgagctgccc aaccttggcc cgtatctcga
     2041 agacctggca cggttggttc cagaagtggc cccggaagcc aagccggtcc cggtcgacgc
     2101 cagactcggc gcggccagac tgctggaggc gctcgccagg ctgctcgaag ggctcggttc
     2161 gaccctggtg ttcgatgacc tccagtgggc ggacagcggc acgcacgagg ttctggcgct
     2221 gttgtgtgct cgggccggcg tcgactgtgt cggtgcatac cgtgtcggag aggcgtcagc
     2281 cgagctacgc gacctggccg cctccttgtc tcggcgtggc gagctgctcg agcttcacct
     2341 gcctggcctc cgtcaggttg atgtggacga cctcctcgcc gcgatgtccg gcgcggacgg
     2401 ccactggccg cggctctccg cctggctgtt cgcacgctcc gccggcaacc ctctgttcgc
     2461 gctcgagacg atcaaggccc tcttcgacac cggcgtcttc ctggcggacg acagcgactg
     2521 gcccgcgaaa ctggagggac tggtcgacgg cctggaagtc agtgccctgc cagcagcggt
     2581 aaccgagctg atccagcgcc gtgtctccgc gctctccggc ggggcgagaa ggctgcttca
     2641 ggtgaccagc gtgctcggtg gggacgccag ccccgagctg ctcacctcgt tggtgggcct
     2701 gtcggattgg ggcttgctgg acgccatcga ggaggcggag gcgcacggtc tgctggcggg
     2761 cgcgagcttc gttcacgatg tgcagtc
//
LOCUS       DAOQYE010000170         2775 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_39454_length_2775_cov_2.325000, whole genome shotgun sequence.
ACCESSION   DAOQYE010000170 DAOQYE010000000
VERSION     DAOQYE010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39454_length_2775_cov_2.325000"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1008)
                     /locus_tag="PKN52_03810"
     CDS             complement(<1..1008)
                     /locus_tag="PKN52_03810"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HNQ99101.1"
                     /translation="MGGLSESPTLADARRLLAKVFGYPEFRGAQAQVIEHLLGGGDAL
                     VLMPTGGGKSLCYQLPALLRPGVGVVISPLIALMHDQVSALRQVGVSAAYLNSSLEPA
                     EARAVERDIAEGRIDLVYVAPERLLTPGFLSLIDRLPLSLFAIDEAHCVSAWGHDFRP
                     EYIGLGVLAERYPDVPRVALTATADAATRREIVEKLHLEEARRFVSSFDRPNIDYQVV
                     EKVDARRQFLAFYREKHVGHAGIVYCLSRKSVDATAAWLVAQGVNAVPYHAGLSAEVR
                     REHQERFLREDGLVVVATIAFGMGIDKPDVRFVAHLDLPKSLEGYYQETGRAGRDGES
                     AD"
     gene            complement(996..2234)
                     /locus_tag="PKN52_03815"
     CDS             complement(996..2234)
                     /locus_tag="PKN52_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HNQ99102.1"
                     /translation="MTTLSFAERLEAYAELLVKVGVNVQPGQKLLVRASVAAAPLVRR
                     VVDIAYRSGSSYVEVLWGDDAVQRSRFLHGSEGSFGIIPQYRADAMNSLAAEGAASLA
                     ILADDPDALAGTLPERFSTYQKAWRPAIRSYSDLAMSDRIPWCVAAAAGPEWARKVFP
                     DLPEAEAVDALWNAIFTATRVLEADPVAAWREHNADLARRKQALNERRYAALRFRAPG
                     TDLRVGLADDHVWDGGGSTLPNGVGFVANMPTEEVFTAPHRERVDGVVRASMPLAYNG
                     ILIDDFSLEFKDGAVVKATAGKGQAALDDILDTDAGARRLGEVALVPASSPIAKADLL
                     FLETLFDENAACHIALGRGYVNCLQGAQEMTGEERLAHGLNDSLAHVDFMIGSSEMDI
                     DGELPDGTTEPVMRKGEWVG"
     gene            2454..>2775
                     /locus_tag="PKN52_03820"
     CDS             2454..>2775
                     /locus_tag="PKN52_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HNQ99103.1"
                     /translation="MALFKDRVDAGEKLAWELGATRDLRGQGTIVLGLPRGGVPVAAA
                     VARQLGAPLDALVVRKLGHPDQPELAIGAVALGGHLFRNDEIIRESSLSDAEVQGIAK
                     RELRE"
BASE COUNT          428 a          937 c         1009 g          401 t
ORIGIN      
        1 gtcagcgctc tcgccgtcac gaccggcacg gcccgtctcc tggtagtaac cctccaggct
       61 cttggggagg tcgaggtggg ccacgaagcg cacgtccggc ttgtcgatcc ccatgccgaa
      121 ggcgatggtg gcgacgacca cgagcccgtc ctcgcgcaag aagcgctcct ggtgctcgcg
      181 gcgtacctcg gcgctcaggc ccgcgtggta cggcacggcg ttgacgccct gcgccacgag
      241 ccaagcggcg gtagcgtcga cgctcttgcg ggacaggcag tacacgatgc cggcgtgccc
      301 gacgtgcttc tcacggtaga aggcgaggaa ctggcgcctg gcgtccacct tctcgacgac
      361 ctggtagtcg atgttaggcc ggtcgaagct cgatacgaaa cggcgcgcct cctcgaggtg
      421 gagcttctcg acgatctcgc ggcgcgtggc ggcgtcggcc gtggcggtga gcgccacgcg
      481 cggcacgtcc gggtagcgtt cggccagcac tccgaggccg atgtactcgg gccggaagtc
      541 gtggccccac gccgacacgc agtgcgcctc gtctatggcg aagagcgaga gcggcaaacg
      601 gtctatgagg gagaggaagc ccggcgtaag caggcgctcc ggagcgacgt acacgaggtc
      661 gatgcggcct tcggcgatgt cgcgctccac ggcgcgcgcc tccgccggct cgaggctcga
      721 gttgaggtaa gcggcgctca cacccacctg gcgcagcgcg ctcacctggt cgtgcatgag
      781 cgcgatcaac ggcgagatga ccacgccgac gcccgggcgc aggagcgcag gaagctggta
      841 gcagagcgac ttgccgcccc ccgtcggcat gaggacgagg gcgtcaccac cgcccaagag
      901 gtgctcgatg acctgtgcct gggcgccgcg gaactccggg tagccgaaga ccttggccag
      961 caggcggcgg gcgtctgcca gggtgggcga ctcgctcagc ccacccactc ccccttgcgc
     1021 atgacgggct cggtcgtgcc gtccggcagc tcgccgtcga tgtccatctc gctcgaaccg
     1081 atcatgaagt cgacgtgcgc gagcgagtcg ttcagaccgt gggcgagccg ctcctcgccc
     1141 gtcatctcct gcgccccctg gaggcagttg acgtaaccgc gaccgagcgc gatgtggcac
     1201 gcggcgttct catcgaagag cgtctccagg aagagcaggt ccgccttggc gatggggctg
     1261 gatgcaggca cgagcgcgac ctcgccgagc cggcgcgcgc cggcgtccgt gtcgaggatg
     1321 tcgtcgagcg ccgcctgccc cttgccggcc gtggccttca cgacggcacc gtccttgaac
     1381 tcgagcgaga agtcgtcgat gaggatgccg ttgtaggcga gcggcatgct ggcgcgcacg
     1441 acgccgtcca cgcgctcgcg gtgcggcgcc gtgaagacct cctccgtcgg catgttcgcc
     1501 acgaacccga cgccgttcgg gagcgtggag ccaccgccgt cccagacatg gtcgtctgcc
     1561 agcccgacac gcaggtccgt gccgggtgcg cggaagcgca gggcggcgta gcggcgctcg
     1621 ttgagggcct gcttgcggcg cgcgaggtcg gcgttgtgct cgcgccaggc ggcgaccggg
     1681 tcggcctcta gaacgcgcgt ggccgtgaag atggcgttcc agagggcgtc gaccgcctcg
     1741 gcctccggca ggtccgggaa caccttgcgg gcccattcgg gccccgcagc ggcggccacg
     1801 caccacggga tgcggtcgct catggccagg tcggagtacg aacggatggc ggggcgccaa
     1861 gccttctggt aggtggagaa gcgctccggc aaggtgccgg cgagagcgtc cgggtcgtcc
     1921 gccaggatgg ccagggaggc cgcgccctcc gccgccaggc tgttcatggc gtccgcgcgg
     1981 tactgcggga tgatgccgaa gctaccctcc gaaccgtgca ggaagcggga acgctggacg
     2041 gcgtcgtcgc cccacagcac ctcgacgtac gagctgccgc tccggtaggc gatgtcgacc
     2101 acccgtctca ccaacggcgc cgccgcgacg ctggcgcgta cgagcagctt ctggccgggc
     2161 tgtacgttga ccccgacctt gacaagtaac tctgcgtacg cctccaggcg ctccgcgaaa
     2221 gaaagagtag tcatgacgag catcgtagca gcgggctgcg ggccgcgccc ggcaaggcgg
     2281 cagatcgcca catcggaccc ggcgggttgt caggtgcggc taggcaagtg gccctggctt
     2341 tcagccggcg ttgccttaac ctcgaagtga cgggcgtcgc gggcgcgctg gaaggcgccg
     2401 gccatcatgc gacgtgctcg caccgtcgcc tcggcgcccg gtaaggagga ggtatggcgc
     2461 tgttcaagga ccgcgtggac gccggcgaga agctcgcttg ggagctcggc gcgaccaggg
     2521 acttgcgggg ccaaggcacc atcgttctcg gcctgccgag gggcggcgtg cctgtggcgg
     2581 ccgcggtggc gcgtcaactc ggcgccccgc tcgacgcgct ggtcgtccgc aagctcggcc
     2641 accctgacca accggagctg gccatcgggg cggtggcgct aggtgggcac ctgttcagga
     2701 acgacgagat catccgggag agctccctga gcgacgcaga agtccagggg atcgccaaac
     2761 gggagctgcg cgagc
//
LOCUS       DAOQYE010000171         2773 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_39489_length_2773_cov_2.955114, whole genome shotgun sequence.
ACCESSION   DAOQYE010000171 DAOQYE010000000
VERSION     DAOQYE010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2773
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39489_length_2773_cov_2.955114"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..853)
                     /locus_tag="PKN52_03825"
     CDS             complement(<1..853)
                     /locus_tag="PKN52_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99104.1"
                     /translation="MIPRWQQVQERRGAAVSTDALFPRIEVRPPYFALSELSLAGLEL
                     RATTSAESPAYDEQGPMPAAELGRHAAIVGLAAAAFQQRDDDRRYYLARSAECRYVAN
                     GAPYGTPVRFTGRVLELDKRTARAAVEAMAAGAPLASFDVRYTVLTSSAFERLFRIRA
                     RPTPYSHSPYGSLLTTAWQGADDAAEQVVDVLPVEACVGHFDGYPALPVAVLMGQLSF
                     LAGRLHGEPYRVVRGEVEASDLVWAGERARFRVTRDGVGLDDDTVGAKRYRCEAWAGD
                     RSVAAMTL"
     gene            complement(1004..1078)
                     /locus_tag="PKN52_03830"
     tRNA            complement(1004..1078)
                     /locus_tag="PKN52_03830"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1044..1046),aa:Val,seq:gac)
     gene            complement(1153..1227)
                     /locus_tag="PKN52_03835"
     tRNA            complement(1153..1227)
                     /locus_tag="PKN52_03835"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1193..1195),aa:Cys,seq:gca)
     gene            1319..>2773
                     /locus_tag="PKN52_03840"
     CDS             1319..>2773
                     /locus_tag="PKN52_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNQ99105.1"
                     /translation="MSLQEPKRFSHLHQHTDYSLLDGAARIKDLISWVKEVSPDRPAV
                     AMTDHGNMHGAVQFYKTAVAAEVKPIIGFEAYVAPGSRFEKRRGSSKLDGGYFHLTLL
                     ARDFKGYQNLCRLSSRAFLEGFYMKPRVDLELLREHSAGVIALSGCLGAQIPRTILDV
                     GPDAGERVLLEHLEIFKENFFVELQDHGLEEQRRLNPILRAFADKHGIGMVATNDGHY
                     VRKTDAHAHDVLLAIGTKALVSEENRMRFPCEEFYVKSPEEMAAVLPESDYPGAIGNT
                     NHVADLCDVQLPIGSKRVYQMPELPLPEGVTLAEQLRVDSYRGLMRRYATADEAFWRG
                     YAATAFVPGAASADLDSVLLGVARAGEASVQPQAEGENFPRYRYEHLERFVAAGGLDE
                     TARTVLERVEFELGVIVAMGFPDYFLIVADFINWAKDHDIAVGPGRGSGAGSIVAYAL
                     GITNLDPLEFELLFERFLNPERVSMPDIDVDFCFE"
BASE COUNT          444 a          973 c          918 g          438 t
ORIGIN      
        1 acagcgtcat ggcggccacc gagcggtcgc ccgcccaagc ctcgcaccgg taacgcttgg
       61 cgccgaccgt gtcgtcgtcg agtccgaccc cgtcgcgcgt cacgcgaaag cgggccctct
      121 cccctgccca tacgaggtcg ctggcctcga cctcgccgcg cacgacgcgg tagggctcgc
      181 cgtggagccg tccggccagg aacgacagct ggcccatcag cacggcgacg ggcaacgccg
      241 gatagccgtc gaaatggccg acgcaggctt cgacgggcag cacgtcgacc acctgctcgg
      301 cggcgtcatc tgcgccttgc catgcggtgg tcagtagcga cccgtagggg ctatggctgt
      361 aaggggttgg tctggccctg atcctgaaca gccgctcgaa cgccgaggag gtcagcacgg
      421 tgtagcggac gtcgaaggag gcgagagggg cgccggccgc catggcctcg accgccgccc
      481 gcgccgtgcg cttgtcgagt tcgagtaccc tgccggtgaa ccgaacgggc gttccgtacg
      541 gcgccccgtt ggcgacgtag cggcactcgg cgctccgcgc gaggtagtag cggcggtcgt
      601 cgtcgcgctg ctggaaggcc gcggccgcca gtccgacgat ggcggcatgc ctgccgagtt
      661 cggccgccgg catcggaccc tgttcgtcgt acgccggcga ctccgcgctg gtcgtggcgc
      721 gcagctccaa cccggcgagg ctgagctcgg agagcgcgaa gtacggtggc cgcacctcga
      781 tcctcgggaa cagcgcgtcc gtcgatacgg cggcgccccg tcgctcctgg acctgctgcc
      841 aacgggggat catcggcggc ccgcccatgt gatttcgttc ggatccgtcc aggcgctccc
      901 cgagcccctt ctagccttca cgctgaccac tcctctgccc caaagtcggt tggcgccgtc
      961 aacataacgc agaacccgcg ctgcgtaagc gcgggttcca agttggtagg cgcggacagg
     1021 attgaactgt cgacctctac agtgtcagtg tagcgctctc ccactgagct acgcgcctgc
     1081 ggtgtgaacc ctcgtctttc gtcgccccgc gtccggcgcc cccttggagg cgagcgccga
     1141 ccgggtggga gatggaggcg ccgaccggaa tcgaaccggt gaatcgaggt tttgcagacc
     1201 tctgccttac cgcttggcta cggcgccaag ctcacagaag gtaccacggg cccgtcgggg
     1261 acgtcaacgc gcctcggggc ggccgagcgc gggagcgccc gggggtatcc tgagacccat
     1321 gtcgctccag gaaccgaagc gcttctcgca cctgcaccaa catacggact actcgctcct
     1381 ggacggcgcc gcgcgcatca aggacctcat ctcgtgggtc aaggaggtct cccccgaccg
     1441 gcccgctgtc gccatgacgg accacggcaa catgcacggc gccgtgcagt tctacaagac
     1501 agccgtggcc gcggaggtca agcccattat cgggttcgag gcgtacgtcg cgccaggctc
     1561 gcgtttcgag aagcggcgcg ggtcgagcaa gctcgacggc ggctacttcc acctgacatt
     1621 gctggccagg gacttcaagg ggtatcagaa cctttgccgc ctgagcagcc gagcgttcct
     1681 ggaaggcttc tacatgaagc ccagggtgga cctcgagctg ctgcgtgagc actccgcagg
     1741 cgtcatcgcc ctttccggct gcctcggagc ccagattccg cgcacgatcc tcgacgtcgg
     1801 ccccgacgcg ggcgagcgcg tgctgctcga gcacctcgag atcttcaagg agaacttctt
     1861 cgtcgagctc caggaccatg ggctggagga gcagcggcgc ctcaacccca tcctgagggc
     1921 gttcgccgac aagcacggca tcggcatggt ggcgaccaac gacggccact acgtccgcaa
     1981 gaccgacgct cacgcccacg acgtcctcct cgccatcggg accaaggccc tggtgagcga
     2041 ggagaaccgc atgcgcttcc catgcgaaga gttctacgtg aagagccccg aggagatggc
     2101 cgccgtgctc ccggagagcg actaccccgg cgccatcggc aatacgaacc acgtggccga
     2161 cctctgcgac gtccagctcc cgatcggctc gaaacgcgtc taccagatgc ccgagctgcc
     2221 gctgcccgag ggtgtcacgc tggccgagca gctgcgagtc gactcctacc gggggctcat
     2281 gcggcgctat gcgaccgcgg acgaggcgtt ctggcggggc tacgcggcga ccgcctttgt
     2341 tccgggcgcg gcgagcgccg acctggactc cgtcctgcta ggcgtggccc gagccggcga
     2401 ggcgagcgtg caaccgcagg ccgagggtga gaacttcccc cgctaccggt acgagcatct
     2461 ggagcgcttc gtggcggccg gtggtctaga cgagaccgcc cgcaccgtgc tcgagcgggt
     2521 ggagttcgag ctcggcgtca tcgtggccat ggggttcccc gactacttcc tgatcgtcgc
     2581 ggacttcatc aactgggcga aggaccacga catcgcggtc ggccctgggc gcggctcggg
     2641 cgccggctcc atcgtcgcgt acgccttggg catcaccaac ctcgaccccc tcgagttcga
     2701 gctcctcttc gagcgcttcc tcaacccgga acgcgtctcg atgcccgaca tcgacgtgga
     2761 cttctgcttc gag
//
LOCUS       DAOQYE010000172         2758 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_39824_length_2758_cov_2.902701, whole genome shotgun sequence.
ACCESSION   DAOQYE010000172 DAOQYE010000000
VERSION     DAOQYE010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2758
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39824_length_2758_cov_2.902701"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1560
                     /locus_tag="PKN52_03845"
     CDS             <1..1560
                     /locus_tag="PKN52_03845"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HNQ99106.1"
                     /translation="IKAVESAQRDAAPSQRLTDRFQRVFSPVVLGVTALMIVVPPLFG
                     APFEASFMRAMVLLVASSPCALALATPSAVLAGIARGARSGVLIKGGAHLENAGMADV
                     VVLDKTGTLTRGRPELTDVIVVGGLGALGGVTAPTDAAALAGDERRLLTLVAGLESRS
                     AHPLAGAILRGAVARLGARLAADAGHAHDPATLPFPAPVLPAPVLPAPSLPEPSLPEP
                     SLPEPSFRPSIPSDDLPSVTEFSELSGHGVTGTVDGVTVLIGNRKLMAAAGVSLPEAA
                     EARAAELEGAGKTTSFVAVGGPATVALGAGSEADADAHGAANDGPSAAPVLTGLLAVR
                     DEPRPEAAATVAELRRLGVKRLVMLTGDHERVARAVGAELGLDEVHAGLLPEDKARIV
                     TKLKGGHHRVIVVGDGINDAPAMAEAHVAVAMGGAASDVALEAADVALMADDLGKLPY
                     AFALSRASRRMIVQNLFVSMGVIALLVPSAVLGLASIGVAVVIHESSTLIVVANALRL
                     LAFKPRHRPAS"
     gene            1600..2520
                     /locus_tag="PKN52_03850"
     CDS             1600..2520
                     /locus_tag="PKN52_03850"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HNQ99107.1"
                     /translation="MQLGFATAIFPDLSLEEVLAFAADEGFKWLEVMCWPTDASDHRR
                     YAGVTHIDVGAFGAADAARVNDLCRKHGVGISGLGYYPNPLAPDEAERDTYVTHLGRV
                     IDAAGLLGVGVVNTFVGRDPSLPIEAQWEQVEAVWRPIVDRAKAAHVRIGIENCPMLF
                     SLDEWPGGKNLAVSPAVWEELFRRLRSESLGLNFDPSHLVWQGIDIDRALRDFAHKLV
                     HVHLKDERVDRDLLHERGILGLGWHFPKIPGLGDIDWSRFFRTLTQIGWDGPVTIEVE
                     DRAFEDSVAGRMEAVRAAGRYLRTVMEFGG"
BASE COUNT          384 a         1013 c          979 g          382 t
ORIGIN      
        1 atcaaggccg tggagtccgc gcagcgcgac gccgccccaa gccagcgcct caccgaccgc
       61 ttccaacgcg tcttctcgcc cgtcgtcctc ggcgtcacgg cgctcatgat cgtcgtgcct
      121 cccctgttcg gcgcgccgtt cgaggcgagc ttcatgcgcg ccatggtcct cctcgtggcg
      181 tccagcccgt gcgcgctcgc gctggccacg ccgtcggccg tcctggccgg catcgcgcgc
      241 ggcgcgcgca gcggcgtgct catcaagggc ggggcgcacc tcgagaacgc gggcatggcg
      301 gacgtcgtcg tcctcgacaa gacgggcacg ctcacgcgcg ggcgccctga gctcacggac
      361 gtgatagtgg taggcggcct aggcgcgctc ggcggcgtga ccgcaccgac cgacgcggcc
      421 gcgctggccg gcgacgagcg gcggctcctc accctcgtgg cgggcctgga gagccgcagc
      481 gcgcatccgc tcgccggggc catcctgcgc ggcgcggttg cgcggctggg cgcgcggctg
      541 gcggcggacg cgggacacgc tcatgacccg gcgacgctcc ccttcccggc accggtcctc
      601 cccgcaccgg tcctccccgc accgagcctc ccggaaccta gcctcccgga acctagcctc
      661 ccggaaccta gcttccggcc gagcatcccg tccgacgacc tcccgtcggt cacggagttc
      721 tccgagctca gcgggcacgg cgtgaccggc accgtggacg gggtcacggt gctcatcggc
      781 aaccgcaagc tcatggccgc ggcgggcgtg agcttgccgg aggcggccga agcgcgcgcg
      841 gccgagctcg agggggccgg caagacgacg agcttcgtgg cggtaggcgg ccccgcgacg
      901 gtcgcgttgg gcgcgggcag cgaagcggac gcggacgcgc acggcgcggc aaacgacggc
      961 ccgtcggcag cgcccgtcct gaccggcctg ctcgccgtac gcgacgagcc gcggccggag
     1021 gcggccgcga ccgtcgccga actgcgccgg ctcggggtga agcgcctcgt gatgctgacg
     1081 ggcgaccacg agcgcgtggc gcgcgccgtc ggcgccgagc tggggctgga cgaggtgcac
     1141 gcggggctcc tgccggagga caaggcccgc atcgtgacca agctcaaggg gggccatcac
     1201 cgcgtgatcg tcgtgggcga cggcatcaac gacgctcccg ccatggccga ggcgcacgtc
     1261 gccgtcgcca tgggcggcgc ggcgagcgac gtggcgcttg aggccgctga cgtcgccctc
     1321 atggcggacg acctcggcaa gctcccgtac gccttcgcgc tctcgcgcgc cagccgccgc
     1381 atgatcgtgc agaacctgtt cgtgagcatg ggggtcatcg cgctgctcgt gccgagcgcc
     1441 gtgctggggc tggcgagcat cggcgtggcc gtggtcatcc acgagtcgag cacgctcatc
     1501 gtcgtcgcga acgcgctgcg cctgttagcc ttcaagccta gacaccgccc ggccagctag
     1561 acaccgcccg gccatcccgg cccggaagga gccaaaagca tgcagctcgg attcgctacc
     1621 gcgatcttcc ccgacctgtc gttggaagag gtcctggcgt tcgccgccga cgagggcttc
     1681 aagtggctcg aggtcatgtg ctggcccacc gacgcgagcg accaccgccg ttacgccggc
     1741 gtgacccata tcgacgtagg cgccttcgga gccgccgacg ccgcgcgcgt gaacgacctg
     1801 tgccgaaagc acggcgtcgg catcagcggc ctcggttact acccgaaccc gctcgccccc
     1861 gacgaggcgg agcgcgacac gtacgtgacg cacctcgggc gcgtcatcga cgccgccggg
     1921 ctcctcggcg tcggggtcgt caacactttc gtcggccgcg atccgagcct gccgatcgag
     1981 gcccagtggg agcaggtcga ggccgtgtgg cggccgatag tcgaccgcgc caaggcggcg
     2041 cacgtccgga tcgggataga gaactgcccg atgctgttca gcctggacga atggccgggc
     2101 ggcaagaacc tagccgtctc gcccgccgtc tgggaggagc tgttcaggcg cctgcggagc
     2161 gagagcctcg gcctaaactt cgacccgtcg cacctcgtgt ggcagggcat cgacatcgac
     2221 cgggccctgc gcgacttcgc gcacaagctc gtccacgtcc acctcaagga cgagcgcgtc
     2281 gaccgggacc tcctccacga gcgcggcatc cttggcctcg gttggcactt cccgaagatc
     2341 cccgggctcg gcgacatcga ctggtcgcgg ttcttccgca ccctcacgca gatcgggtgg
     2401 gacggccccg taaccatcga ggtggaggac cgcgcgttcg aggacagcgt cgcgggccgc
     2461 atggaggcgg tgcgcgccgc ggggcggtac ctgaggaccg tgatggagtt cgggggctga
     2521 gcaggttcca gttcagggcg gccggctcag cgcggccttg tgcggtgcgc ccggctcgtg
     2581 gcgcctagcg cggcgccgcg aacacgagcg tccagaacag gtccgtctcg cccgccccga
     2641 gctcgctcac ctcggagcgc atcatgttct cgcagtgtcc ggggctgtcc atcaaggcct
     2701 gcacgacgac ctcgggcgtt ggttggttcc acgccacgtt ctccgccacg acggacca
//
LOCUS       DAOQYE010000173         2750 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_40004_length_2750_cov_2.686085, whole genome shotgun sequence.
ACCESSION   DAOQYE010000173 DAOQYE010000000
VERSION     DAOQYE010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2750
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40004_length_2750_cov_2.686085"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1667
                     /locus_tag="PKN52_03855"
     CDS             <1..1667
                     /locus_tag="PKN52_03855"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HNQ99108.1"
                     /translation="SNADAPARPIEPPMAERTPLTPEGAVSLGASVGLRVPSDLAGGV
                     AARLNAVAAEAERWRPAVGRDVVPFVDFELPRREVTGGGTGSGVATYTTFASTGPTRP
                     KRTADDAGDLFDLAAGIRERRLSPVALAEEKLRAIAALNGELIAYLTIANDRALEDAE
                     RAERELAQGVYRGPLHGVPIAHKDLIPTKGIRTTYHTAAFKDNVPEEDAPIVERLARA
                     GTVLLGKANTLELGSGDGDVFGLARNPWDPARQVGGSSSGSAVAVAAGLAAAATGTDA
                     GGSIRIPAAFCGIVGVKPTAGLVEVSKGTNGISVTGPMTRTTLDAAVLLEAMSGQAGL
                     VERVTGDVAGLTVGVPVDWLDTPIEDEVASSMHQAVEVLRGLGASVREVRLPHASASE
                     VLGGVVTHVDNFGKYRFLLDQDAQLGKFVHELLLAAELYPAAAYRLGQRLRRLMLEEV
                     TAAHAAADILITPMVPYRAARLGETALRIGSTTVNPRTGQGRFTRLSNLTGFPSLSVP
                     TGLDSNGMPLSVQLHGRPFEEAVILSAARAIELHNRQRTARPRLHA"
     gene            1696..>2750
                     /locus_tag="PKN52_03860"
     CDS             1696..>2750
                     /locus_tag="PKN52_03860"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HNQ99109.1"
                     /translation="MLLNREKTRQLMREEGLDALVVAYTENVIVFTDFMHVNSNRIKP
                     RLYYVVFFADEARAPVFLVPHQDFDDAKRQTWIEDVRPTAEYVYPGRPNVIVDKVGAV
                     CDIVRAAGLERGVIGFENASLPYDVHQALVGGLPNATFKPASDLLARLRAIKSEEELK
                     RIRKAMDATQAGARAIMNNVRAGITEKELAALAKEACLAAGGEYVDFLIVGTAENGAI
                     VHGTPTDRALEDGDIMRFDLGAVYGSYPGDFARTYAVGTNPREVDARRYSAVRAAVEA
                     GVAAVRPGATAGDVYDAMLTAGQAIDPQLYREHAGHGVGLEIHEEPMIYKGSKFVLEP
                     GMVVMIECGRYILGEAG"
BASE COUNT          444 a         1002 c          936 g          368 t
ORIGIN      
        1 cttcgaacgc ggacgcgccg gcacgtccga tagaaccgcc catggctgag cgcacgcctc
       61 tcaccccgga aggcgccgtc tcgttaggtg cgtcggtcgg cttgagggtg ccgagcgacc
      121 tcgccggcgg cgtggcggca cgcctcaacg cggtggcggc cgaggccgag cgctggcggc
      181 cggccgtcgg ccgcgacgtc gtccctttcg tcgacttcga gctaccgcgc cgggaggtca
      241 ccgggggcgg aaccggctcg ggcgtcgcta cgtacaccac tttcgcgtcg accggcccga
      301 cccggccgaa gcgcacggcc gacgacgccg gcgacctctt cgacctggcc gcgggcatcc
      361 gcgagcgccg gctgtccccc gtcgctctgg ccgaggagaa gctgcgcgcc atcgccgcgc
      421 tcaacggcga gctgatcgcc tacctgacga tcgcgaacga ccgggcactg gaggacgcgg
      481 agcgagctga gcgcgagttg gcgcaagggg tctaccgggg accgctccac ggggtgccca
      541 tcgcccataa ggacctcatc cccacgaagg ggatccgcac gacgtatcac acggcggcct
      601 tcaaggacaa cgtgcccgag gaggacgcgc ccatcgtcga gcgcctggcg cgcgccggca
      661 cggtgctgct tggcaaggcc aacacgctcg agctcggcag cggcgacggc gacgtcttcg
      721 gcctggcacg caacccatgg gatccggcga ggcaggtggg gggctcgagc tccggctcgg
      781 ccgtcgccgt ggccgcgggc ctggcggcag cggccaccgg cacggacgcc ggcggctcca
      841 tccgcatccc cgctgcgttc tgcggcatcg tcggcgtcaa gccgaccgcc ggcctggtcg
      901 aggtgagcaa gggcaccaac ggcatctcgg tgacgggccc catgacgagg acgacgctgg
      961 acgcggccgt gctgctcgag gccatgagcg gccaggccgg cctcgtggag cgcgtgactg
     1021 gcgacgtcgc cggtctgacg gtcggcgtgc ccgtcgactg gctcgacacg cccatcgagg
     1081 acgaggtcgc gagcagcatg caccaggccg tcgaggtgct gcgcggtcta ggtgcgagcg
     1141 ttcgcgaggt gcgactgccg cacgccagcg ccagcgaggt gctcggcggg gtcgtcacgc
     1201 acgtcgacaa cttcggtaag taccgcttcc tcctcgacca ggacgcccag ctcggtaagt
     1261 tcgtgcacga gctcctcctc gcggccgagc tctacccggc ggccgcgtat cggctcggcc
     1321 aacggctgcg gcggctcatg ctcgaggaag tgacggcggc gcacgcggcg gccgacatcc
     1381 tgatcacgcc catggtcccg taccgagccg cgcggctcgg cgagacggcg cttcgcatcg
     1441 gttccaccac ggtcaacccg cgcaccggtc aggggcgctt cacgcgcctc agcaacctca
     1501 ccggcttccc gagcctgagc gtgccgaccg gcctcgactc caacggcatg cctctctccg
     1561 tccagctcca cggccgcccc ttcgaggagg ccgtcatcct ctcagccgcc agggccatcg
     1621 agctccacaa ccgccagcgc accgcgcgcc ccagactcca cgcctgagcc aggcaacgaa
     1681 agaaggccac gaaacatgct cctgaaccgc gagaagaccc gccagctgat gcgagaagag
     1741 ggcctcgacg ccctcgttgt cgcctacacc gagaacgtca tcgtcttcac ggacttcatg
     1801 cacgtgaaca gcaaccgcat caagccgcgc ctctactacg tcgtcttctt cgccgacgaa
     1861 gcgcgtgcgc ccgtcttcct tgtcccgcac caggacttcg acgacgccaa gcgccagacg
     1921 tggatcgagg acgtgcgccc gaccgccgag tacgtctacc ccggtcgccc caacgtcatc
     1981 gtcgacaagg tcggcgccgt gtgcgacatc gtgcgcgccg ccgggctcga gcgcggcgtg
     2041 atcggcttcg agaacgcctc gctgccgtac gacgtccacc aggcgctcgt cggcgggctg
     2101 ccgaacgcga ccttcaagcc cgcgagcgat ctgctggcca ggctcagggc catcaagagc
     2161 gaagaggagc tgaagcgcat ccgcaaggcg atggacgcca cgcaggcggg cgcgcgggcg
     2221 atcatgaaca acgtccgggc cggcatcacg gagaaggagt tggccgctct cgccaaggaa
     2281 gcctgcctcg cggccggcgg cgagtacgtc gatttcctca tcgtcggcac ggccgagaac
     2341 ggggccatcg tccacggcac cccaaccgat cgcgccctcg aggacggcga catcatgcgc
     2401 ttcgacctgg gcgccgtgta cggctcgtac ccgggcgact tcgcacgcac gtacgcggtg
     2461 ggaacgaacc cgcgcgaggt ggacgcgcgg cgttactccg ccgtcagggc cgccgtggag
     2521 gccggcgtag ccgcagtcag gccaggtgcc accgccggcg acgtctacga cgccatgctg
     2581 acggccggcc aggcgatcga cccgcagctg taccgcgagc acgccggtca cggcgtcggc
     2641 ctggagatcc acgaggagcc catgatctac aagggttcga agttcgtcct agagccgggc
     2701 atggtcgtga tgatcgaatg cggccgctac atcctgggcg aggccggata
//
LOCUS       DAOQYE010000174         2750 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_40007_length_2750_cov_2.605937, whole genome shotgun sequence.
ACCESSION   DAOQYE010000174 DAOQYE010000000
VERSION     DAOQYE010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2750
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40007_length_2750_cov_2.605937"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(77..424)
                     /locus_tag="PKN52_03865"
     CDS             complement(77..424)
                     /locus_tag="PKN52_03865"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNQ99110.1"
                     /translation="MHPFAVLGDPVRRRLVEVLAGGERTAGELVELIGGEFGITQSAV
                     SQQLHILREQGFAAVRAEGRRRIYALDPRPLRVVGAWAARYRPFWEGALDSLENEVEL
                     GKSERAEPGRPAN"
     gene            complement(434..1108)
                     /locus_tag="PKN52_03870"
     CDS             complement(434..1108)
                     /locus_tag="PKN52_03870"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNQ99111.1"
                     /translation="MAFRFEDHLARMDRRVSSGTRGGMETAIVQAGRTYATDALDLWR
                     ALTTPERLSRWFLPVTGELRLGGRYQFEGNAGGTVQECVEPELIKATWEFGGGISWLT
                     LRLIPQPAGTRLELEHEAPLMPGFSDVYGPGAVGVGWDGGFLGLTLHLADPDAAKPPE
                     ADPEWPMSAEGKAFYGAAAAAWGEADAASGTAADQARARAETTRKFYTGELQGMGAPE
                     GQSATE"
     gene            1372..1536
                     /locus_tag="PKN52_03875"
     CDS             1372..1536
                     /locus_tag="PKN52_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99112.1"
                     /translation="MEPSHVLDVAGLPHHHRDPFDRLLVAQAMALDVPLMTADPVLDA
                     YPPNLIRLRG"
     gene            complement(1606..>2750)
                     /locus_tag="PKN52_03880"
     CDS             complement(1606..>2750)
                     /locus_tag="PKN52_03880"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNQ99113.1"
                     /translation="PHAAHGGFGAIVTVSPWQLQQERAQLQAQGAGSGGAEAPVPSGG
                     AVVANGLEGGPWVALGDLGAYLRGSLEERAATVVGSAALALAFAVGLALLLARVIAKP
                     IQRVAVAANSVAAGDLTARVSVAPGHRGSKDETVRLAESFNVMADNLERLERQRRDMV
                     ADVAHELRTPLTVLRGRMEAMEDGVVPLTLEEIGDMHSQVLVLTRLVEDLRVLSLADA
                     GRLNLSPQDLDLTEMARSAATAHRDLAANKGVELITVGEDPVHAQLDPERMLQVLGNL
                     LDNAVRHTPAGGRVTLSARREGGEAVLEVADTGPGIPEGAEERVFERFYRADESRSRG
                     AETGGSGLGLAIVKAIVEAHGGSVSAGRADGGGASFRVAVPAKADD"
BASE COUNT          379 a         1034 c          914 g          423 t
ORIGIN      
        1 gcggggtcgc cgaccagcct cgctcgggcc ctccggcgtg ggccggcgcc gcccgccgcc
       61 cgcctgacca cctcgcctag ttggccggcc ttcccggctc cgcgcgctcc gacttgccca
      121 gctccacctc gttctccaag ctatccaggg caccttccca gaacggtcgg tagcgcgccg
      181 cccaggcgcc gacaacacga aggggccgag ggtccagggc gtagatcctg cgccgaccct
      241 cggcgcgcac ggcggcgaac ccctgttccc tgaggatgtg cagttgctgc gacaccgcgg
      301 actgggtgat cccgaactcg ccgccgatca gctccaccag ttcgcccgca gtgcgctcac
      361 cgccggcgag cacctcgaca aggcggcggc gcaccgggtc gcccagaacc gcgaaagggt
      421 gcatgcccat ccgctactcc gtcgcggact ggccttccgg agcgcccatg ccctggagtt
      481 cgcccgtgta gaacttccgg gtcgtctcgg ccctggcgcg ggcctggtcg gcggccgtgc
      541 cggacgcggc gtcagcctcg ccccacgccg ctgcggcggc cccgtagaac gccttgccct
      601 cggccgacat gggccactcc gggtctgcct ccggcggctt ggcggcgtcg gggtcggcga
      661 ggtgaagcgt caggccaagg aagccgccgt cccagccgac tccgacggcc ccggggccgt
      721 acacgtcgct gaaaccgggc atgagcggcg cctcgtgctc caactccagt cgggtcccgg
      781 ccggctgggg tatgagtcgc agcgtcagcc agctgatacc gccgccgaac tcccaggtag
      841 ccttgatcag ctcgggttcc acgcactcct ggaccgtgcc gccggcgttg ccctcgaact
      901 ggtagcgacc gccgaggcgt agctcaccgg tgacggggag gaaccatctg gatagccgct
      961 ccggcgtcgt gagggcgcgc cacaggtcga gcgcgtccgt ggcgtacgtg cgcccggctt
     1021 ggacgatggc ggtctccatg ccaccgcgcg tgccgctgga aacgcggcga tccatgcggg
     1081 ctaggtggtc ttcgaacctg aaagccatgc tcgactcctt cgcatgcggc tacgttgttg
     1141 tcgcgctacc gcgttccgga cggagtgagc cggacgcatg cgcccgggcc gcgaccgccg
     1201 ctttacttat attagcacgt actaatatga cggctcgaac gagttgttgc tttcgggggc
     1261 gagctcatgg gagatagcca tcaagtactg gctaggccgc ctaccactgc cctaccccca
     1321 tcgacccacg tacccgacag gatcaggtcg accgctgtca cccccgtggc ggtggagccc
     1381 tcccacgttc tcgacgttgc cggcctgccc catcatcatc gcgatccgtt cgaccgcttg
     1441 ctggtagccc aggccatggc gctggacgtg cccctgatga ccgccgaccc ggtcctcgac
     1501 gcctacccgc ccaacctcat caggctgcgc ggctgacggc gcccgacccg gttgctgagt
     1561 ggctgaggca tgttggccct cagggtagcg ggggcgcgtt agccgtcaat cgtctgcctt
     1621 ggcgggcacc gcgaccctga acgacgcccc tcccccgtcc gcgcgcccgg cgctcacgct
     1681 gccgccatgg gcctcgacga tggctttgac gatcgccagt cccaggccgc tgccgccggt
     1741 ctccgcaccg cggctccgac tctcgtccgc ccggtagaac cgctcgaaca cgcgctcctc
     1801 ggcaccctcc gggatgccgg ggcccgtgtc ggcgacctcg aggaccgctt ctccgccctc
     1861 tcgcctggcg ctgagcgtca cgcggccgcc ggccggagtg tggcgcacgg cgttgtcgag
     1921 caagttgccc aggacctgaa gcatgcgctc ggggtccagc tgcgcgtgaa ccgggtcctc
     1981 gccaacggtg atcaactcga cacccttgtt ggcggccagg tcgcggtgtg ccgtagccgc
     2041 cgagcgcgcc atctccgtca ggtcgaggtc ttgcgggctg aggttcaacc ggcccgcgtc
     2101 ggccagcgac aggacccgca ggtcctcgac cagacgcgtg aggacgagga cttgggagtg
     2161 catgtcgccg atctcctcga gcgtcagggg cacgacgccg tcctccatgg cctccatgcg
     2221 tccacgcagg acggtcagcg gggtgcgaag ttcgtgggcc acgtcggcga ccatgtcgcg
     2281 gcgctgccgc tccaagcgtt cgaggttgtc ggccatcacg ttgaacgact ctgccagccg
     2341 cacggtctcg tccttgcttc ccctgtgtcc gggagcgacg ctcacccgag cagtgaggtc
     2401 gccggccgcc acgctgttcg ccgccaccgc cacgcgctgg atgggtttgg cgatgacgcg
     2461 ggcgagaagc agggccaggc cgacagcgaa ggctagggcc aacgccgcgg agccgacgac
     2521 ggtggccgcc cgctcctcga gcgaaccgcg caggtaagcg cccaggtcgc cgagcgccac
     2581 ccacgggccg ccctccagcc cgttcgcaac gaccgctccg ccgctcggca cgggcgcctc
     2641 ggcgcccccg gatccggcac cctgcgcctg gagctgggca cgttcctgct gcagttgcca
     2701 tggcgacacg gtcacgatgg ccccgaagcc gccgtgcgcc gcgtgagggg
//
LOCUS       DAOQYE010000175         2745 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_40103_length_2745_cov_2.973606, whole genome shotgun sequence.
ACCESSION   DAOQYE010000175 DAOQYE010000000
VERSION     DAOQYE010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40103_length_2745_cov_2.973606"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(60..1697)
                     /locus_tag="PKN52_03885"
     CDS             complement(60..1697)
                     /locus_tag="PKN52_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99114.1"
                     /translation="MDQTGRFQRNQELRRDGDVVTSLGVDVVTGLPVPVYDFAGSPTL
                     APGQSDLEGIPAILAASTGEGRGCLVVAYPNDATLVAPGESVVDDKFALRAATILRDA
                     AKRGIVHGDLGPGRFLWSGGALYLEGYGVPWRPGQVAPQKGGPMPAGLAAALQADVRS
                     AAKALAQLGERTLSAEVMAVLRAAAAGSSNTDAGSLQATLRRLAGGAVTVPSAGFADI
                     VLPTTTGGVGSASAARDFDELELPSSGTPPTHRSAPETAGPNAGRPDEARRDTPRQDD
                     SLLDLDFGSEAVTPDSGFDPDVRASLAQTPVPGTPRRAPAAHTPPTGPRTASDQPGRT
                     EQAGPGSLPTPLEPDPITVLSDPGTRSASPGSPKLVEPRRSTGGGEAAFVKHLPPGAT
                     YRPGSLDDAPRPAPIRVTDTFSPVIHRRSWRGPALLVLLLLVVALGTFLALRAQRASN
                     LGQVGPGAVRHLVDVRVDPTNLPPVSLVVDVSPDGSGYSPGTIIGSVPRRVAFDANGT
                     WVVHAQFQGRSSERVTLRVPDDVVITLSFPEPTPPSP"
     gene            1800..>2745
                     /locus_tag="PKN52_03890"
     CDS             1800..>2745
                     /locus_tag="PKN52_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HNQ99115.1"
                     /translation="MDVTRERVLEALRGVNDPELHQDLVTLGMVDSVEVDGVAVKLQI
                     TLTTPACPLKAKIEQDVRAAIAGHTGTTSVEITFGSQVRGPAQMPLPGVRNVIAVGSG
                     KGGVGKSTVAANLAAALHLEGAQVGLLDADIYGPSQSKMYGVEGKRLMADDEKHILPL
                     RNHGVKVISIANLVEDGQALTWRGPILHGTLTQLLKQTVWGELDYLVVDLPPGTGDVQ
                     LSLSQLVPVTGMVLVTTPQAVALVDVKRAYTMARKTHIPVLGVVENMSYYALPDGTRD
                     YIFGEGGATAWAESERLPILGRVPITRKLRESGDSGVPI"
BASE COUNT          421 a          938 c         1005 g          381 t
ORIGIN      
        1 gtcgggcagg tgcagtgtgc gggcgggtgg tgcaggtggg cggagagcgt gcgccgccat
       61 catggcgagg gcggggtggg ctccgggaag gagagcgtga tcacgacgtc gtccgggacg
      121 cgcagcgtca cgcgctcgct ggagcgcccc tggaactggg cgtgcacgac ccaagtgccg
      181 ttggcgtcga acgcgacgcg ccgcggcacc gagccgatga tcgtgccagg actgtagccg
      241 gacccgtcgg ggctcacgtc gaccacgagg ctgaccggcg gcaggttggt ggggtcgacg
      301 cgcacgtcga ccaggtgcct gaccgctccg ggacccacct gtccgaggtt gctcgcgcgt
      361 tgggcgcgga gcgccaggaa cgtcccgaga gcgaccacca acagtaggag cacgagcagc
      421 gcgggtccgc gccacgagcg cctatggatg acgggagaga acgtgtcggt caccctgatg
      481 ggcgccggac ggggtgcgtc gtctagcgag ccgggtcggt aggtggcgcc cggcggcagg
      541 tgcttcacga acgcggcctc gcccccgccg gttgaccggc gcggctcgac cagcttcggg
      601 ctgccggggc tggcggagcg cgtgccaggg tcgctgagga ccgtgatggg atccggctcc
      661 agcggggtcg gcagactgcc gggtccagcc tgctcggtcc gcccgggctg gtcggacgcc
      721 gtgcgcggac cggtcggagg agtgtgggca gcgggagcgc gacgtggggt acccgggacc
      781 ggggtctgcg cgaggctggc gcgcacgtcg gggtcgaacc cgctatcggg cgtgacggcc
      841 tcggagccga agtctaggtc caacaagctg tcgtcctgac gaggggtatc ccgccgggct
      901 tcatccggcc ggccggcgtt ggggccggcc gtctccggcg ccgaccgatg ggtcggcggc
      961 gtaccagacg aagggagctc gagctcatcg aaatcgcggg cggccgacgc ggagccgacg
     1021 ccccctgtcg tggttggcag tacgatatcg gcgaagccgg cggatgggac cgtgacggca
     1081 ccgccggcca ggcgccttag cgtcgcctgc aggctgccgg cgtcggtgtt gctcgagccg
     1141 gccgccgccg cgcgcagtac cgccatgacc tcggcgctaa gggtccgctc gcctagctgg
     1201 gcgagcgcct tggcggcgct gcggacatcc gcctggagcg cggctgccaa gcccgcgggc
     1261 atgggcccgc ccttctgcgg ggccacctga cccggtcgcc acggcacgcc gtagccttcg
     1321 aggtagaggg ccccacccga ccagaggaag cggcccggtc cgaggtcgcc gtggacgatg
     1381 ccgcgcttgg cggcgtcacg caggatcgtg gcggctctca gggcgaactt gtcgtctacc
     1441 acgctctccc ctggagctac gagcgtagcg tcgttcgggt aggccaccac taggcagccg
     1501 cgcccctcgc cggtggaagc cgcgaggatc gccgggatgc cttcgagatc ggactgaccc
     1561 ggagccagcg ttggcgaacc ggcgaagtcg tagacgggaa cgggcagccc ggtaaccacg
     1621 tcgacgccga ggctggtgac gacgtcacca tcgcgcctca gttcctggtt gcgctggaag
     1681 cgtcccgttt ggtccacgcg cctgtgagta tagccgtgct caccatgggt cacaaggcga
     1741 cttgggtaaa ccgcctggac gcaagaggac ctcccgcgct ggtacgctct tgttcagcta
     1801 tggacgtaac ccgcgaacgc gtactcgaag ccctcagggg cgttaacgac ccggaactcc
     1861 accaggacct ggtgacgcta ggcatggtcg actccgtcga ggtcgacggc gtggccgtca
     1921 aactccagat caccctcacc acgccggcct gcccgctcaa ggcgaagatc gagcaggacg
     1981 tgcgggcggc catcgccggc catacgggca cgacctccgt agagatcacc ttcggttcgc
     2041 aggtcagggg tcctgcccag atgcccctgc cgggggtgcg caacgtcatc gcggtcggct
     2101 cgggcaaggg cggggtaggg aagtcgaccg tcgccgccaa cctggctgcc gcgcttcacc
     2161 tcgagggcgc gcaggtcggc ctgctggacg ccgacatcta cggccccagc cagagcaaga
     2221 tgtacggcgt cgagggcaag cgcctcatgg cggacgacga gaagcacatc ctgcccctgc
     2281 gcaaccacgg cgtgaaggtc atctcgatcg ccaacctcgt cgaggacggc caggcgctca
     2341 cctggcgcgg ccccatcctc catggcaccc tcacacagct cctcaagcag accgtgtggg
     2401 gcgagctcga ctacctcgtc gtcgacctgc ccccgggcac cggggacgtg cagctctccc
     2461 tctcgcagct cgtacccgtg acgggcatgg tgctcgtcac cacgccgcag gccgtcgcgc
     2521 tcgtcgacgt caagcgcgcc tacaccatgg cccgcaagac gcacatcccc gtgctcgggg
     2581 tcgtcgagaa catgagctac tacgccctgc cggacggcac gcgcgactac atcttcggcg
     2641 agggcggcgc caccgcctgg gcggagagcg agcggctgcc gatactcgga cgcgtgccca
     2701 tcacgcgcaa gctgcgcgag agcggcgaca gcggcgtgcc gatcg
//
LOCUS       DAOQYE010000176         2745 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_40104_length_2745_cov_2.954647, whole genome shotgun sequence.
ACCESSION   DAOQYE010000176 DAOQYE010000000
VERSION     DAOQYE010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40104_length_2745_cov_2.954647"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="PKN52_03895"
     CDS             complement(<1..662)
                     /locus_tag="PKN52_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99116.1"
                     /translation="MFRNTTSKPPITRPVAHAVVAVAALLLALVPSAALAQHSEHAAT
                     GRIVGSASALALNNAMRELWAQHMEWTYAAVTAFATESPSFEATAARLMSNQVDIGDA
                     IKPFYGDAAGEALTTLLQAHISAAVDVVSAAKAGDSAATDTAIAAAYANAQEIADFLA
                     LANAYWPQATVRDMLKGHIDTTLVYATALLQGNYADGIAEYGTAEAHMMMLADALTDG
                     LIA"
     gene            957..1298
                     /locus_tag="PKN52_03900"
     CDS             957..1298
                     /locus_tag="PKN52_03900"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNQ99117.1"
                     /translation="MADPTPDAKRGTVDLAILATLELQARYGLEILEEVQRATGGGLA
                     FKEGSLYPALHRLVKQRWVVSRWEDSDAGGAPRKYYELTDDGRRALARKREEWRAVRD
                     AMEVFLVVGNA"
     gene            1298..2467
                     /gene="rmuC"
                     /locus_tag="PKN52_03905"
     CDS             1298..2467
                     /gene="rmuC"
                     /locus_tag="PKN52_03905"
                     /inference="COORDINATES: protein motif:HMM:NF014680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HNQ99118.1"
                     /translation="MGPIDQYIHRATLGLPRRQRLDTAAELRVHLNARAASLSSQGLD
                     PAEAEHLAVERMGPVDPLNRALLGHLFTPRLGWVVAAALAVAFGAWLVLNHLFAPAPV
                     VRARQVVPGDLTPLLGDFTGLTVTAPSGTRSLAFGLAQGGGDVSTWAVGGLGEYAAGF
                     RPNQRIGLDVTVGFPAAALAPEPCADGSRSLYVGDGWGWSFRCLPNVTPSGEWRHVGE
                     GAALTLDSWRPLLVYEGPGEESPELVVMYLPGETFFSAAIENDPGLIEDGSLRKVILA
                     TPTTLIALLKAVAYGWQQEQVATGAREINRLGREIYERFSVVADHIGRAGTNLSKAVE
                     AHNDAVRSIETRLMPSFRRFKDLGVSTSRELPEAMDEVRVTARETIAGTEPGEKT"
BASE COUNT          406 a          911 c          996 g          432 t
ORIGIN      
        1 gcgatgaggc cgtcggtgag ggcgtcggcg agcatcatca tgtgcgcctc ggccgtgccg
       61 tactccgcga tgccatcggc gtagttgccc tgcagcagcg cggtcgcgta cacgagcgtc
      121 gtgtcgatgt gccctttgag catgtcgcgg acagtggcct gcggccagta cgcgttcgcg
      181 agcgccagga agtcggcgat ctcctgcgcg ttcgcgtagg cggccgcgat ggcggtgtcg
      241 gtcgccgcgg agtcgccggc cttggcggcg ctcacgacgt cgacggcggc ggagatgtgc
      301 gcctggagca gcgtcgtcag cgcctctccc gccgcgtcgc cgtagaacgg cttgatggcg
      361 tcgccgatgt cgacctggtt gctcatgagc cgggccgccg tcgcctcgaa cgagggcgac
      421 tccgtggcga acgccgtgac ggctgcgtac gtccactcca tgtgctgggc ccacagctcg
      481 cgcatggcgt tgttcagggc cagggcgctc gcgctgccga cgatgcgccc ggtcgcggcg
      541 tgctcggagt gctgggcgag cgccgccgac ggcacgagcg ccaggagcag tgcggctacg
      601 gcgacgaccg cgtgggcgac ggggcgggtg atgggcggct tgctggtggt gttacggaac
      661 atgacgacct ccatggtcgg tgactgtgct ggagcgaggt ccggtggcgg gacttgcttg
      721 tcccgttccc gctcgctcta tccctttaga tgcaggaagt gggcatggcg ttcccgggtg
      781 tgggtgggcg aggcggtccg cgggtgctct aacaatgtcc gcggagtcga gtggcagcgg
      841 tccgagcccc gtgtggcagg acttggcggc gggcgccgac ttgacagccg ggccgccgcc
      901 gctctatata ggaacgcaag atacttgcca agcaaggtag aggacggagg ccttacatgg
      961 ctgacccaac cccggacgct aagagaggca cggtcgacct ggccatcctg gccacgttgg
     1021 agcttcaggc ccggtacggc ctggagatcc tcgaggaggt gcagcgggcc acgggcggcg
     1081 ggctcgcctt caaggagggg agcctctatc cggccctgca ccggctcgtg aagcagcggt
     1141 gggtggtctc gcgctgggag gacagcgacg ccggcggcgc gccaaggaag tactacgagc
     1201 tgaccgacga cgggcggcgc gcgctggcgc gcaagcgcga ggagtggcgc gccgtgcgcg
     1261 acgccatgga ggtgttcctg gtcgtgggga acgcctgatg ggccccatcg accagtacat
     1321 ccaccgggcc acgctcggcc tgccgcggcg gcagcggctc gacacggcgg ccgagctgcg
     1381 cgtccacctg aacgcccgcg cggcctcgct ctcgtcccag gggctcgacc cagccgaggc
     1441 cgagcacttg gcggtcgagc gcatgggacc cgtcgacccg ctcaaccgcg ccctgcttgg
     1501 ccacctcttc acgccgcgcc tcgggtgggt ggtggccgcc gcgctcgccg tggccttcgg
     1561 cgcgtggctc gtcctgaacc acctgttcgc cccggccccc gtcgtgcggg cacgccaagt
     1621 ggtgccgggc gacctgacgc cgctcctcgg cgacttcacg ggcctcaccg tcacggcccc
     1681 gagcggcacg cgctccctcg cgttcggcct cgcgcagggc gggggcgacg tctccacgtg
     1741 ggccgtcggc ggactcggcg agtacgctgc cggcttccgg ccgaaccagc gcatcggcct
     1801 ggacgtcacc gtcgggttcc ccgccgccgc ccttgcgccc gagccctgcg cggacggctc
     1861 gcggtccttg tacgtcggcg acggctgggg ctggagcttc aggtgcctgc cgaacgtcac
     1921 gccgagcggc gagtggcggc atgtcggcga gggagccgcg ctgaccctcg acagctggcg
     1981 accgctgctc gtctacgagg ggcccggcga ggagtcgccc gagctcgtgg tcatgtacct
     2041 gcccggcgag accttcttca gcgccgccat cgagaacgac ccgggcctca tcgaggacgg
     2101 cagcctccgc aaggtgattc tcgccacccc cacgacgctc atcgcgcttc tcaaggccgt
     2161 ggcctacggg tggcagcagg agcaggtggc caccggcgcg cgggagatca accgcctggg
     2221 ccgggagatc tacgaacgct tctccgtcgt cgcggaccac atcgggcggg cggggacgaa
     2281 cctctcgaag gcggtcgagg cgcacaacga cgccgtccgc tccatcgaga caaggctcat
     2341 gccgtccttc cgccgtttca aggacctggg cgtctcgacg agccgggagc tgcccgaggc
     2401 catggacgag gtccgggtga cggcccgcga aacgatcgcg ggaacggaac cgggtgaaaa
     2461 gacctgaagt aaggaagata agaagagcgg aagataggaa tggaggaaag attccttgtc
     2521 ttgtccgctc ttcctgactt ctgctcttcc tgacttcaca accgcaaagc cggcaaagga
     2581 tctcaatgcc tgacgggatg cgcccccggt cacgggcata tcagaagatg cgccgctgca
     2641 gcttgctctt gtgcatcacc gtcgccgcga tctcctcgat cgacatggtc gtgatgttga
     2701 ggtaggggat gttcttggag acgtagagga actccgccag ggaga
//
LOCUS       DAOQYE010000177         2743 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_40152_length_2743_cov_3.063616, whole genome shotgun sequence.
ACCESSION   DAOQYE010000177 DAOQYE010000000
VERSION     DAOQYE010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2743
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40152_length_2743_cov_3.063616"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1056
                     /locus_tag="PKN52_03910"
     CDS             <1..1056
                     /locus_tag="PKN52_03910"
                     /inference="COORDINATES: protein motif:HMM:NF013798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HNQ99119.1"
                     /translation="HDLKLQLYWEDALTGAPDDESYPSHLPVLSATGWEAAPVDARVA
                     LARALLDIAAAKREFARVARTGDRVMAHERLAGLLKALAGLPVGADRDREEAEIMELK
                     RRLEAGDHVMAARQSEVDARNYVKSATSAKRSQMFKLELERRLRSGAPTTPAGGPGAA
                     QAFGGPGDVLFQADVGAARVQIVKGDITRQVVDAVVNSTSRAMIGAAGVDGALARAGG
                     PKHLAAMRAIGGLAYGQAVFTPAFGIPARYVIHTAAHPWEGGGKEVDILRQCYDAVFA
                     LAESLGAKTLAMPAIGTGSYRFPATVAARIATDATEAWLARGAFELVRLVVVEDFVAT
                     AYLREASDRVARLNAGV"
     gene            1049..2623
                     /locus_tag="PKN52_03915"
     CDS             1049..2623
                     /locus_tag="PKN52_03915"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HNQ99120.1"
                     /translation="MFDRKRSTSEESPIEVFWVEPAAHGAAGRLGLTFAPGKKGAGIA
                     TDVELDRDLRQDMARLRDVHGTDLLVSLMEDHEYVQLRIPELFEVAAACGIRTERLPI
                     KDVHTPRPGEEGAVDALIGAVRASLRRGEAVVIHCRGGHGRTGTIAALTLVSYGHRAE
                     DAITLVREAQKHTVEHRRQAEYVSETERRWLQARTWTAPDRVRGALVGLATGDALGTA
                     VEFRASGTFEPVTDMVGGGVFGLRPGEWTDDTSMALCLAESIVETGGFDPLDQVRRYV
                     RWWREGHLSSNGVCFDIGTTTRAGLAAFERTSRPFSGPTSEHTAGNGSLMRLAPVPMA
                     CYADPGEAMRLAGESSRTTHGAPECVAACRYFAGLLVGAIDGAAKEELLAPNYSPVAP
                     GARRAELGPDAGATADLTLAPGVAEVASGSFLRREPPEIAAHGYVVSTLEAALWAFAR
                     TDTFEEGALKAVNLGNDADTVGAVYGQLAGAFYGEGAIPERWRRKLALRDEIGRLADG
                     LVALGGKARPPAGTRD"
BASE COUNT          407 a          924 c         1044 g          368 t
ORIGIN      
        1 cacgacctga aactccaact ctactgggag gacgccctga ccggcgcccc cgacgacgag
       61 agctacccct cgcatctccc ggtactgagc gccacggggt gggaggcggc gccggtggac
      121 gcgcgggtgg cgctcgcacg cgcgctcctc gacatcgcgg cggcgaagcg ggagttcgcc
      181 cgcgtggcgc gtacgggtga cagggtcatg gcgcatgagc ggctcgccgg gttgctgaag
      241 gcgctcgccg gactgccggt cggagcggac cgtgaccgcg aagaagccga gatcatggag
      301 ctgaagagac gactggaggc gggcgatcac gtcatggccg cgcggcagtc cgaggtggac
      361 gcgaggaact acgtcaagag cgccacgtcg gcgaagcgct cgcagatgtt caagctcgaa
      421 ctggagagac gtctgcggtc cggcgcaccg acgacgccgg ccggtggccc cggcgcggcg
      481 caggcgttcg gcggccccgg cgacgtcctg ttccaagccg acgtcggtgc cgcccgcgtc
      541 cagatcgtga agggcgacat cacccgtcag gtggtggacg cggtcgtcaa ctccacgagc
      601 agggccatga tcggcgccgc cggtgtggac ggtgcgctcg cccgcgccgg agggccgaag
      661 cacttggcgg ccatgcgggc catcggcggc ctcgcctacg gccaggctgt gttcacgccc
      721 gcgttcggca tccccgcccg gtatgtcatc cacaccgccg cgcacccgtg ggaaggcggc
      781 ggcaaggagg tcgatatcct gcgccagtgc tacgacgcgg tgttcgcgtt ggccgagagc
      841 ctcggcgcga agacgctggc catgcccgcc atcggcacgg gcagctaccg cttccccgcg
      901 accgtcgccg cgcggatcgc taccgacgcg accgaggcct ggctggcccg gggcgccttc
      961 gaactggtcc ggctcgtggt cgtggaggac ttcgtcgcga ccgcctacct gcgggaggcg
     1021 tccgacaggg tggccaggct gaacgccggt gtttgacagg aagcggagca cctctgaaga
     1081 gagccccatc gaggtgttct gggtggagcc cgccgcccac ggtgcggcgg gccggctggg
     1141 cctgacgttc gcgcccggca agaagggcgc gggcatagcg acagacgtgg agttggaccg
     1201 cgacctgcgc caggacatgg cgcgcctgcg cgacgttcac ggcacggacc tgctggtctc
     1261 gctcatggag gaccacgagt acgtccagct gcgcatcccc gagctgttcg aggttgcggc
     1321 ggcctgcggc atccgtaccg agcgcctgcc gatcaaggac gtgcacacac cgcggccggg
     1381 cgaggagggg gcggtcgacg ccttgatcgg cgcggtccgc gcctcgctcc gcaggggtga
     1441 agcggtcgtg atccactgcc ggggcggcca cgggcgcacg gggacgatcg cggccttgac
     1501 gctcgtgagc tacggccacc gcgccgagga cgccatcacg ctcgtgcgcg aggctcagaa
     1561 gcacactgtt gagcaccggc ggcaggcgga gtacgtgagc gagacggagc gacggtggtt
     1621 gcaggcccgg acctggaccg cgcccgaccg cgtgaggggc gccctcgtcg gcctggccac
     1681 cggcgacgcc ctcgggaccg ctgtcgagtt ccgggcctcc gggaccttcg aaccggtgac
     1741 cgacatggtg ggcggcggag tgttcggctt gaggccgggc gagtggacgg acgacacgag
     1801 catggcgctc tgcttggccg agagcatagt cgagacgggc ggtttcgacc cgctcgacca
     1861 ggtgcggcgc tacgtgcgct ggtggcgaga ggggcacctc tcctccaacg gggtctgctt
     1921 cgacatcggc accaccacga gggccggtct cgccgctttc gagcgcacga gccgaccgtt
     1981 cagcggtccg acgtcggagc acacggcggg caacggcagc ctcatgcgcc tcgctccggt
     2041 gcccatggcg tgctacgccg atccgggcga ggcgatgcgg ctcgccggcg agagctcgcg
     2101 cacgacccat ggcgcgcccg agtgcgtggc ggcgtgccgg tacttcgccg ggttgctggt
     2161 cggcgccatc gacggcgccg ccaaggagga gttgctcgcg ccgaactaca gcccggtagc
     2221 gccgggagcc cgacgtgccg aactcggacc ggacgcgggt gcgacggcgg atctgaccct
     2281 cgcgcctggt gtcgccgagg tcgcgtccgg gtcgttcctg cggcgcgaac cgcccgagat
     2341 agccgcgcac ggttacgtgg tcagcacttt ggaggcggcg ctctgggcct tcgcgcgcac
     2401 ggataccttc gaggagggtg cgctgaaggc cgtgaacttg ggcaacgacg ccgataccgt
     2461 cggagccgtg tacgggcagc tggcaggggc cttctacggc gaaggcgcga tccccgagcg
     2521 gtggcgccgg aagttggcgt tgcgcgacga gatcggacgg ttggccgacg ggctcgtcgc
     2581 cttgggcggg aaggcacggc cacccgccgg cacgcgggac tgaggccgag cccgacggtg
     2641 gagccagccg agcgtcagcc gaagtcgagc cacatgacga cgcgactggc ggctggcatg
     2701 gtcgcgccgg ctctcatgag agccggcgcg accatgccag ccg
//
LOCUS       DAOQYE010000178         2732 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_40401_length_2732_cov_3.228241, whole genome shotgun sequence.
ACCESSION   DAOQYE010000178 DAOQYE010000000
VERSION     DAOQYE010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40401_length_2732_cov_3.228241"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..549
                     /locus_tag="PKN52_03920"
     CDS             <1..549
                     /locus_tag="PKN52_03920"
                     /inference="COORDINATES: protein motif:HMM:NF015279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HNQ99121.1"
                     /translation="VAVLDAAGFDTVFIETVGVGQSEVDIVTAADTTVVVLTPGQGDA
                     VQAFKAGIMEIADVFCVNKYDHPGGDRLRREIRAAQELGDHPEGWLPPIATTVASKGE
                     GVDDLVRLLDEHRAHLLASGGLELARRRRARSEVRAALGERLRRELLDGEEDAVDAVL
                     AGRLTPEDVVDDLLRTLVAGGG"
     gene            602..1084
                     /gene="coaD"
                     /locus_tag="PKN52_03925"
     CDS             602..1084
                     /gene="coaD"
                     /locus_tag="PKN52_03925"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HNQ99122.1"
                     /translation="MIHAVYPGSFDPLHNGHVDLIRRAARIYEKLTIAVLRNDSKPNA
                     LFDTEERVAIIREVVEGMEGVEVDSFDGLLAEYTRKLGATVIVKGLRAISDFEYELQM
                     AHLNRQLNPNAETTFIMTATRWSYVSSTRVKELARYGADVAKLVPRATLRRLHEKLPH
                     "
     gene            1192..2184
                     /locus_tag="PKN52_03930"
     CDS             1192..2184
                     /locus_tag="PKN52_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HNQ99123.1"
                     /translation="MSYLLRGVAAGGGIRVVAADTTGLVNGAVEKHGATPTAGAALGR
                     TLTGALLLSHVLLKNSRDRVTLRLRGDGPLGGVIADAGLDGTVRGYVSNPHVDLPLRD
                     DGKLDVGGGIGREGEISVVRSHAPYGDPYGSSSDLVSGEVAEDIATYLARSEQIPSAV
                     LLGVYFDEHRRVRRSGGVILQALPGADEGALTLLEANVKALGQLTTAMAEAPILDVVR
                     DQLLWGMDFELLTDPPMRVAFACRCSDEKALEALAYFTPAERKEMAEQDGGAEVVCHW
                     CGEKRWVGAEAILGIAGAEVRCPDCGTLWYRDGQTRMVRDAELCACGRPVVLPN"
     gene            2233..>2732
                     /locus_tag="PKN52_03935"
     CDS             2233..>2732
                     /locus_tag="PKN52_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNQ99124.1"
                     /translation="MRPGLVLLLLFQMVLWGSAYPMIKLGLGGMSADHLTLLRHLVAS
                     VAFLPILVVMRSRLLPRRQDVPYLFLLGLLGYTIYHLALNFGELHVSAGAASLIIGTA
                     PAMTALLATALMRERLPALGWIGSLVSFAGVALIVLGDAGGGVSFNAWSWLVVISAVA
                     SSFYTVL"
BASE COUNT          397 a          967 c          940 g          428 t
ORIGIN      
        1 gtggccgtcc tcgacgcggc ggggttcgac accgtgttca tagagacggt cggcgtgggc
       61 cagtcggaag tcgacatcgt cacggcggcc gacaccacgg tcgtcgtgct gacgcccggt
      121 cagggcgacg ccgtgcaggc cttcaaggcc ggcatcatgg agatcgccga cgtgttctgc
      181 gtaaacaagt acgaccaccc gggcggcgac cggctgcgca gggagatccg agccgcgcag
      241 gagctgggcg accatccgga aggctggctt cctcccatcg ccacgacagt agcgtcgaag
      301 ggcgagggcg tcgacgacct cgtgcggctc ctggacgagc accgcgccca tctgctggcg
      361 agcggcgggc tcgagctcgc gcgcaggcgc agggcgcgtt ccgaggtcag ggccgcgctg
      421 ggcgagaggt tgcgccggga actcctggac ggcgaggagg acgccgtcga cgccgtcctg
      481 gccggacgcc ttacgccaga ggatgtcgtg gacgacctgc tccgcacgct cgtggcgggc
      541 ggcggctgaa gtaccgccgt tccgctcccc gcgaccgccg ggccgctaca ctgacgccac
      601 catgatccac gccgtctacc ctggtagctt cgatccgctg cacaacggcc acgtcgacct
      661 catccgccgc gccgcgcgca tctacgagaa actgaccatc gccgtgctgc gcaacgactc
      721 gaagcccaac gcgctcttcg acacggagga gcgcgtcgcc atcatcagag aggtcgtcga
      781 gggcatggag ggcgtggagg tcgactcgtt cgacggtctg ctcgccgagt acacgcgcaa
      841 gctgggggcc acggtgatag tcaagggcct ccgcgccatc tcggacttcg agtacgagct
      901 gcagatggcg cacctcaacc gccagctcaa ccccaacgcc gagaccacgt tcatcatgac
      961 ggccacgcgc tggtcgtacg tgtccagcac gcgcgtcaag gagctcgcgc gttacggcgc
     1021 cgacgtagcc aaactcgtgc cccgcgccac gttgcggcgt ctgcacgaga agctgcccca
     1081 ctaggctact cgcccggtgc ctgggcagcc catatactct ctggtcgcgc ccacgggcgc
     1141 cgctgtccgc caatgcgcca tgggtcgctc gagcggcacg agagggagtc aatgtcctac
     1201 ttgcttcggg gagtcgccgc gggcggcggc atccgcgtgg tcgcggccga caccaccggc
     1261 ctggtcaacg gagccgtcga gaagcacggc gccacgccca ccgccggcgc cgccctgggg
     1321 cgcaccctca cgggggcgct cctcctgtct cacgtcctct tgaagaacag ccgcgaccgc
     1381 gtcacgctcc gcctgcgggg tgacggcccg ttgggcggcg tcatcgccga tgccggcctc
     1441 gacgggacgg tgcgcggcta cgtcagcaac ccgcacgttg acctcccatt gagggacgac
     1501 ggcaagctgg acgtcggggg tggcatcggg cgcgagggcg agatcagcgt cgtgcgctcg
     1561 cacgcaccgt acggcgaccc gtacggcagc tctagcgacc tcgtgagcgg cgaggtggcc
     1621 gaggacatcg ccacgtacct cgcgcgctcc gagcagatcc cttccgccgt cctgctgggc
     1681 gtctacttcg acgagcacag gcgcgtgagg cgctccggcg gcgtcatcct ccaggcgctg
     1741 cccggggcgg acgagggcgc tctgacgctc ctcgaggcca acgtcaaggc gcttgggcag
     1801 ctcacgacgg ccatggccga ggcgcccatc ctggacgtcg tgcgcgatca gctgctgtgg
     1861 ggcatggact tcgagctcct caccgacccg cccatgcgcg tggcgttcgc ctgtcggtgc
     1921 tccgacgaga aggcgctgga agccctggcc tacttcacgc ccgcggagcg caaggagatg
     1981 gccgagcagg acggcggcgc ggaggtcgtc tgccattggt gtggcgagaa gcgctgggtg
     2041 ggtgccgagg ccatcctggg catcgccggc gccgaggtgc gctgccccga ctgcggtacc
     2101 ctctggtacc gggacggtca gacgcgcatg gtgcgggacg ccgagctatg cgcctgtggc
     2161 cgcccggtgg tcttgccgaa ctgaactcat gcccagttga gccatgccca actgacttag
     2221 cggaggcgcg ccatgcggcc gggtctcgta ctactactgc tcttccagat ggtgctttgg
     2281 ggtagcgcct accccatgat caagctcggg ctgggcggca tgtccgccga ccacctgacg
     2341 ctgctcaggc acctcgtggc ctcggtggcc ttcctgccca tcctcgtcgt catgcgctcg
     2401 cggctgctcc cgcggcggca ggacgtgccg tacctgttcc tgctcggcct gctcggctac
     2461 accatctatc acctggcgct gaacttcggc gagctgcacg tgagcgcggg cgcggcgagc
     2521 ctcatcatcg gcacggcgcc cgccatgacc gcgctcctcg ccacggccct gatgcgcgag
     2581 aggctgccgg cgctaggctg gatcgggtcg ctcgtgtcgt tcgccggcgt cgccctcatc
     2641 gtcctcggcg acgcgggcgg cggcgtctcg ttcaacgcct ggtcgtggct cgtggtgatc
     2701 tcggccgtcg cgtcgtcctt ctacaccgtc ct
//
LOCUS       DAOQYE010000179         2711 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_40910_length_2711_cov_2.987952, whole genome shotgun sequence.
ACCESSION   DAOQYE010000179 DAOQYE010000000
VERSION     DAOQYE010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40910_length_2711_cov_2.987952"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(50..1849)
                     /locus_tag="PKN52_03940"
     CDS             complement(50..1849)
                     /locus_tag="PKN52_03940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HNQ99125.1"
                     /translation="MLHTLELRDFAIVDELAVELHPGLNVLTGETGAGKSIVVDALEL
                     LGGGRPDLSMIRAGAESALVQGTFIGTPFESAGRRIARGGRHAARLDGELVTVSELTE
                     AVGGALRVYGQHSAHQLLAPAAQLTQLDRLLTPEAVADLDRHKALFAESNAVATRLEE
                     LRTSERERSRRLDGLRFELSEIAAAKVRAGEDEELETELAGLRHAERIVTGGGRALQA
                     LGGEDGSALALAAEALRELEGAGRFSPALAALARDLRELVSGLGAVSGEVEGFLADFQ
                     ADPARLDSVQARLAALDGLKRKYGPLLQDVIAYRDAALAEAAGLEGADDEIERLSARL
                     GSLDAELVALGERLTAARRHAARALEEQALPLLRQLGLTQARFAVEIGKSARRTRTGY
                     DDVTFEFGANPGEPTRAVHEVASGGELSRIMLALHLVTGSDLPTLVFDEVDAGVGGET
                     ANHVGALLARLARSRQVLVVTHLAQVAAFADAHFKVEKQAAADRTVARVVRLGDAERT
                     TELARLLSGTLTDTSMRHALELRAMATGSAGTTGRLHNSFPCPRPSRAENTTVVFPPL
                     PSSVRYCGSVEAAPGATSISVVFASVPSLRHNS"
     gene            1940..>2711
                     /locus_tag="PKN52_03945"
     CDS             1940..>2711
                     /locus_tag="PKN52_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HNQ99126.1"
                     /translation="MRTELTGRVGSGERVTWVECPRDAWQGLRVAPRTAEKVGHLRGL
                     VAAGFDHLDLGSFVSPKAVPQMADTEEVLAALAAPPAVDVLCIVGNLRGLERAARATG
                     VTSVGYPLAVSDTFQHRNVGTSVAEAWDVVAELLPRARAAGIEVVVYVSMGFGNPYGD
                     PWRPAVTAAAVARLRSLGVARVALADTLGNADPELLGAVLAEVDDPGTLGLHLHSRPH
                     AWVDKVETALAYGVRWLEGALAGVGGCPFAADELVGNLP"
BASE COUNT          372 a          944 c          995 g          400 t
ORIGIN      
        1 cgcccgccaa tggctggctg tactttatcg ccgatgatgg cctgcacggt caggaattgt
       61 ggcgcagcga tggcacagaa gcgaacacca cgctgataga ggtagcccca ggcgcggctt
      121 caacggaccc gcagtatctg acgctgctgg gtaacggcgg aaacaccacc gtggtattct
      181 ccgcgcgcga tggcctcggg catgggaaag aattgtggag cctgccggtc gttccggcgg
      241 acccggtggc catggcgcgc agctccagcg catgcctcat ggacgtgtcc gtcagcgtcc
      301 cggacaggag gcgggcgagc tcggtcgtgc gctccgcatc gcccaagcgg acgacccggg
      361 ccaccgtcct gtcggcagcc gcctgcttct cgaccttgaa gtgggcgtcc gcgaaggcgg
      421 ccacctgcgc taggtgcgtg accaccagca cttgcctcga acgtgccagg cgggcgagga
      481 gcgcgccgac gtggttggcc gtctcgccgc ccaccccggc gtccacctcg tcgaagacga
      541 gggtcggcag gtcggagccg gtcacgaggt gcagggcgag catgatgcgc gagagctcgc
      601 cgcccgaagc gacttcgtgc acggcgcggg tcggctcgcc ggggttggcg ccgaactcga
      661 acgtcacgtc gtcgtacccg gtgcgcgtgc gcctggcgga cttcccgatc tcgacggcga
      721 agcgcgcttg ggtcagtccc agctggcgca gcagcggcag cgcctgctct tccaaggcgc
      781 gcgcggcgtg gcggcgcgcc gccgtcaggc gctcgccgag cgcgaccagc tcggcgtcga
      841 ggctaccgag tcgcgcggac aggcgctcga tctcatcgtc ggcgccctcc aacccggcgg
      901 cttccgccaa cgcggcgtcg cgatacgcga tcacgtcttg gaggagcggg ccgtacttgc
      961 gcttcaggcc gtcgagcgcc gcgaggcggg cctggacgct gtcgagccgc gccgggtcgg
     1021 cctggaagtc ggccaggaag ccttcgacct cgccgctcac ggcccccagg ccggagacga
     1081 gctcccggag gtcgcgcgcg agcgcggcga gcgcggggga gaagcgtccg gcgccttcga
     1141 gctcgcgcag cgcctccgcg gccagcgcca gtgcgctgcc gtcttcccct cccagcgcct
     1201 ggagcgccct gccgccgccg gtgacgatac gttcggcgtg gcgcagaccc gccaactccg
     1261 tctcgagttc ctcgtcctcg cccgcgcgca ccttggcggc ggcgatctcg ctcagctcga
     1321 accgcagccc atccagtcga cgcgaacgct cccgctcgga ggtccgcagc tcctcgagcc
     1381 gcgtcgcgac ggcgttcgac tcggcgaaga gcgctttatg gcggtccagg tccgctacgg
     1441 cctcgggcgt gagcagccgg tcgagctggg tcagttgcgc cgcgggcgcc aggagctggt
     1501 gggccgaatg ttgcccgtag acccgcagcg cacctccgac ggcctcggtt agctccgaga
     1561 cggtcaccag ctcgccgtcg agccgcgctg cgtggcggcc gccacgcgcg atgcgtcgtc
     1621 cggccgactc gaagggggta ccgatgaacg tgccctgcac cagtgcgctc tccgcgccgg
     1681 ccctgatcat gctcaagtcg ggccggccgc cgcccaggag ttcgagcgcg tcgacgacga
     1741 tggacttgcc tgcgccggtc tcgccggtca gcacgttgag gccggggtga agctccacgg
     1801 ccaactcgtc cacgatcgcg aagtcacgca gttccagcgt gtgaagcacg tcatcaagta
     1861 taggggcggg tcgctgccgg tggaccaggg ctgctgccta ggcgcgctcg ggcttgggcg
     1921 cgctcggtag actcgcttcg tgcggacgga gcttaccggt cgagtcggtt caggcgagcg
     1981 cgtcacgtgg gtcgagtgcc cgcgcgacgc atggcagggc ctgcgggtcg ccccgcgaac
     2041 ggcggagaag gtcgggcacc tgcggggact ggtggcagcg gggttcgacc acctcgacct
     2101 cgggtcgttc gtgtcgccca aggccgtgcc gcagatggcc gacacggagg aggtgctcgc
     2161 cgcgctcgcg gcaccacccg ccgtcgacgt gctgtgcata gtcgggaact tgaggggcct
     2221 agagcgagcg gcgcgggcga ccggcgtcac gagcgtcggc taccctctgg cggtcagcga
     2281 cacgttccag caccggaacg tcggcacctc ggtcgccgag gcgtgggacg tcgtggccga
     2341 gctgctgccc agagcgcggg cggcgggcat cgaggtggtc gtctacgtct ccatgggctt
     2401 cggcaacccc tacggcgacc cgtggcgccc cgccgtcacg gcagcggccg tggcgcgcct
     2461 caggagcttg ggagtggctc gcgtcgccct tgccgacacc ctcggcaacg cggacccgga
     2521 actgctcggc gctgtgttgg ctgaggtcga cgacccaggg acgttggggc tgcacctgca
     2581 ctcgcggccg cacgcatggg tggacaaggt ggagacagcg ttggcttacg gggtgaggtg
     2641 gctcgagggc gcgctggccg gggtcggcgg ctgcccattc gcggccgatg agctcgtcgg
     2701 caaccttccg a
//
LOCUS       DAOQYE010000180         2703 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_41133_length_2703_cov_2.344789, whole genome shotgun sequence.
ACCESSION   DAOQYE010000180 DAOQYE010000000
VERSION     DAOQYE010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2703
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41133_length_2703_cov_2.344789"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1683
                     /locus_tag="PKN52_03950"
     CDS             <1..1683
                     /locus_tag="PKN52_03950"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HNQ99127.1"
                     /translation="VDRLVAERGEITDELELIGTYQPLLRDLAPLQGPLDDSKYLTAV
                     AFLIEQDGLEKVKAKIGEQLGGAAAFAARPYKGELLALAVTLRSRVAELRSALGRLGI
                     SEVSLPDSYAGQGVAKAAHMMEERARVLPQRLESIRTDLAKLASQHGARLAAIDRIAH
                     NSRDRYARLEDLAEGRYGFALQGWVPSAQRENVVASLTKQFGGDVVIAAREADAHHDK
                     NVPVKFENPAWVRPFTGLLALFAPPKYGSFDPSWTLAVFFPLYFGIVVGDIGFGILFA
                     VIAFWLRKRGEAGKKLDLGPLNIVVPPGALKPISTVILWCAAWSIVWGFMFGEFFGNF
                     LEHFPAGRPVFYTTLHHHEGYGLINVPLFRVEVFTPLLLLSIGFGVLQVVGGWVIRVI
                     YGAKHHDMKHVFEGIGMAGGLIAIVLFAWAYLSGALSPLVTAIVAIGFLVFLVCVVLA
                     KMPLMLVELISNSGNILSYLRLFAVGLSAALVANLATDLGFALSGTLPVLGPILGIVV
                     GLVVHLTAITLTIIGHTLQPLRLQYVEFFTKFGFYDESGRPYRPFKLLGGKS"
     gene            1680..1991
                     /locus_tag="PKN52_03955"
     CDS             1680..1991
                     /locus_tag="PKN52_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HNQ99128.1"
                     /translation="MNKAVRVLSTISLFTIFGAVAFAQSAGGPSVGAGLIALGAGLAI
                     GLAAIAVGIAQAAIGSAGAGLLAERPEAFGQVLIYLVIPETLIIFGFVIAFFLNNQIG
                     G"
     gene            1994..2584
                     /locus_tag="PKN52_03960"
     CDS             1994..2584
                     /locus_tag="PKN52_03960"
                     /inference="COORDINATES: protein motif:HMM:NF014093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HNQ99129.1"
                     /translation="MADLSALLEKEASTEIETIRSEARDRASEIVGRAREEASSLEAA
                     RARASKAQGEAMLVRAKSAAQLEAASLRLKAQYEGVEGAFAAAKAKLDELMADQSAYA
                     PVFGKLLAEALGGAGGQAVKSVVVAAADRAMAEKAVAEAGLKAAVETADDVKGGVRLR
                     TVDNSIIENTLHGRLEALRGELASEVSAALFGAKAG"
BASE COUNT          412 a          971 c          854 g          466 t
ORIGIN      
        1 gtcgaccggc tcgtggccga gcgtggagag atcaccgacg agctcgaact catcggcacg
       61 taccagccgc tcctgcgcga cttggcaccc ctccagggcc cgctcgacga ctccaagtac
      121 cttaccgccg tcgcgttcct gatcgagcag gacggtctgg agaaggtcaa ggccaagatc
      181 ggcgagcagc tgggcggcgc cgcggcgttc gcggcccggc cgtacaaggg cgagttgctc
      241 gccctcgccg tgacgttgcg ctcgcgcgtc gcggaactgc gtagcgccct cggtcgcctc
      301 ggcatctccg aggtctcgct accggacagc tacgccggtc aaggcgtggc caaggcggcg
      361 cacatgatgg aggagcgcgc cagggtgcta ccccagcgcc tcgagtccat ccgcaccgac
      421 ctcgccaagc tcgcgtcaca gcatggtgcc cggctggcgg ccatcgaccg catcgcccac
      481 aacagccgcg accgctacgc gcgcctcgag gacctcgcgg aaggccgcta cggcttcgcc
      541 ctgcagggct gggtgcctag cgcgcagcgc gagaacgtgg tggcgtcgct cacgaagcag
      601 ttcggaggcg acgtcgtgat cgcggcgcgc gaggccgacg cccaccacga caagaacgtc
      661 ccggtcaagt tcgagaaccc cgcctgggtg cggccgttca ccggcctgct tgcgctcttc
      721 gcgccgccca agtacggcag cttcgacccg tcctggaccc tcgccgtctt cttcccgctc
      781 tacttcggca tcgtcgtagg cgacatcggc ttcggcatcc tcttcgccgt catcgcgttc
      841 tggctgcgca agcgcggcga ggcgggcaag aagctcgacc tcggcccgct caacatcgtc
      901 gtgccgcctg gcgcgctcaa acccatcagc acggtcatcc tctggtgcgc tgcctggagc
      961 atcgtctggg gcttcatgtt cggcgagttc ttcggcaact tcctcgagca cttcccggcg
     1021 ggcaggcccg tgttctacac gacgctgcac caccatgagg gctacgggct catcaacgta
     1081 cccctcttcc gcgtcgaggt gttcaccccg ctgctgctcc tcagcatcgg cttcggcgtg
     1141 ctgcaggtcg tcggcggctg ggtcatccgg gtcatctacg gcgccaagca tcacgacatg
     1201 aagcacgtct tcgagggcat cggcatggcc ggcggcctca tcgcgatcgt gctcttcgcg
     1261 tgggcctacc tgagcggcgc cttgagcccg ctcgtcacgg cgatcgtcgc catcgggttc
     1321 ctcgtcttcc tcgtgtgcgt cgtgctggcg aagatgccgc tcatgctcgt cgagctcatc
     1381 tcgaactccg gcaacatcct cagctacttg cgactcttcg cggtcgggct gtcggcggct
     1441 ctcgtggcga acctcgccac ggacctcggc ttcgccctgt ccggcacgct ccccgtgctc
     1501 ggacccatcc tgggtatcgt cgtcggtctg gtggtccacc tgacggctat caccctcacg
     1561 atcatcgggc atacgctgca acccttgcga ttgcagtatg tcgagttctt caccaagttc
     1621 ggcttctacg acgaaagcgg ccgaccgtac agaccgttca aactcctagg aggaaagtca
     1681 tgaataaggc agttcgcgtt ctctccacca tctccctctt caccatcttc ggcgccgtgg
     1741 ccttcgccca gtcggccggc ggccccagcg tcggcgccgg cctcatcgcc ctcggtgccg
     1801 gcctcgccat cggcctcgcc gccatcgccg taggtatcgc ccaggccgcc atcggcagcg
     1861 ccggcgccgg gctcctggcc gagcgtcccg aggccttcgg tcaggtcctg atctacctcg
     1921 tcatccccga gacgctcatc atcttcggct tcgtcatcgc gttcttcctc aacaaccaga
     1981 tcggcgggta aacgtggctg atctttcggc tctgctcgag aaagaggcga gcaccgagat
     2041 cgagaccatc cgctccgagg cgcgagaccg cgcctcggag atcgttggcc gcgcgcgcga
     2101 ggaggcgtcc tccctcgagg ccgcccgcgc ccgcgccagc aaggcgcagg gtgaagcgat
     2161 gttggtgcgc gccaagtcgg cggcgcagct cgaggcggcc tcgttgcgcc tcaaggcgca
     2221 gtacgagggc gtcgagggcg cgttcgctgc cgccaaggcc aaactggacg agctcatggc
     2281 cgaccagagc gcctacgccc ccgtcttcgg caagctgctc gccgaagcgc tcggcggcgc
     2341 gggcggtcag gccgtcaagt ccgtcgtagt ggccgcggcc gaccgcgcca tggccgagaa
     2401 ggccgtcgcg gaggcgggcc tgaaggcggc cgtcgagacc gccgacgacg tcaagggcgg
     2461 cgtacggttg cgcacggtcg acaacagcat catcgagaac actctccacg gccgcctgga
     2521 ggcgctacgt ggcgagctag cgtcggaggt ctcggcggcg ctcttcggcg ccaaggcggg
     2581 ttgacgaggc atgtctagcg attacggcta catcaacgcg cgcgtcaagg gcatgaaggc
     2641 caagctcctc ggacaggcgt tctaccagac cgctctcgac gcgagcgact tcaagacctt
     2701 cac
//
LOCUS       DAOQYE010000181         2699 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_41247_length_2699_cov_2.139561, whole genome shotgun sequence.
ACCESSION   DAOQYE010000181 DAOQYE010000000
VERSION     DAOQYE010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2699
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41247_length_2699_cov_2.139561"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="PKN52_03965"
     CDS             complement(<1..304)
                     /locus_tag="PKN52_03965"
                     /inference="COORDINATES: protein motif:HMM:NF013486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HNQ99130.1"
                     /translation="MRASMRAAGVDAWLATTGDAHLSEYLSERWQTRAWLSGFKGSAG
                     RFVVTADQAGLWVDPRYHMRADTETAGTAIEVFKEGKPETPELTAWLSASLPTGSVV"
     gene            544..2097
                     /locus_tag="PKN52_03970"
     CDS             544..2097
                     /locus_tag="PKN52_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNQ99131.1"
                     /translation="MSLLVAFGLLGSAFAQQPGSTLVVGMSILPNTIDSGDANDANAI
                     AVTRQIVERLVELAPGNEGLVPGLASSWEANADSTEWTFHLLQGVKFHDGTPFNAEAV
                     KFSLDRWNDGANPYHFGAEGKAYVGWRNLFGGFLGDGSAVSEVVVDDEYTVTIKLTRS
                     ISFLPSLLAGGYFGFDSPAAVMAAGANYGTPAVGSVGTGPYRFERWEEGSRVVLTANP
                     EYRDGPPATETVVFVGVSDPTARLAQLKAGAIDIAAAISPTDLGSVTSDPNLELVQSA
                     GGLNTSYLAIHQNQKPMDDVRVRQAVAYALDRTAIVDAFYAGQAVAANDILPDSLWGH
                     ADGVDAYPYNPEKAKELLAEAGYPNGFDTELWYRNSGLEPVIAEAIASYLADVGIRAA
                     LKTEDWAAFLADYMAGNFALYTLGWNADFADPDTFIYTFYGPQAVRRFGWDDPAVVAL
                     TQQAREVPSQDERSELYAQVISAAHDAMPMLPLAHQSQFFVVRKGVTGLVLNPLGALP
                     TLTSTSKAQ"
     gene            2231..>2699
                     /locus_tag="PKN52_03975"
     CDS             2231..>2699
                     /locus_tag="PKN52_03975"
                     /inference="COORDINATES: protein motif:HMM:NF040248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ99132.1"
                     /translation="MLGIIPVLLGVTFLVFVINRVVPSNPAVAILGDKGTVESQRQLA
                     EELGLNRPIWFDVEGYRATGKFGQLFDSQYFKYMGELLTGNLGKSFMTRAAVTDSLKV
                     RFPATMELAIFSIIIGGILGIVLGVIAALRRGSWIDTGMLFIAVSGVSFPVFWT"
BASE COUNT          452 a          950 c          813 g          484 t
ORIGIN      
        1 cgacgacgct accggtcggg aggctcgcgc tgagccatgc cgtgagttcc ggcgtctccg
       61 gcttcccctc cttgaagacc tcgatggccg tgccggccgt ctcggtgtcg gcacgcatgt
      121 ggtagcgcgg gtctacccag agcccggcct ggtcggcggt aacgacgaac cgacccgctg
      181 agcccttgaa gccgcttagc caggcgcgcg tttgccaacg ttcggagagg tactcgctca
      241 ggtgggcgtc gccggtggtc gccaaccagg catccacccc cgcggcccgc atgctggccc
      301 tcaagcggtc gattctcgct gtcgcgttcg gcatcctggc tccaatcatc gcctcggagg
      361 ggtcggacta cacacccgac gcgccttcag acctttctca tactgttgac aatctagcat
      421 gcccaaagct acgcttcccc cattcagttc tggtgagtgg ggtcggccat gcctgcgcaa
      481 gttcgctcag cagcccccac cgcaaggttc gaagggagaa gcgcaacggt gaaaagagtt
      541 ctgttgtccc tcttggtcgc gttcgggctg ctcggcagcg cgttcgcgca acagcccggc
      601 tctaccctcg tggtcggcat gagcatcctg cccaacacga tcgactcggg tgacgccaac
      661 gatgccaacg ccatcgcagt cacccgccag atcgtcgaac gcctcgtcga gttggccccc
      721 ggcaacgaag gcctcgtccc cgggctggcc agcagctggg aagcgaacgc cgactccacg
      781 gagtggacgt tccacctcct ccaaggagtc aagttccacg acggcacccc gttcaacgcg
      841 gaggccgtca agttcagcct cgaccgctgg aacgatgggg ccaaccctta ccacttcggc
      901 gccgagggca aggcctacgt gggttggcgc aacctgttcg gcggcttcct cggtgacggc
      961 tcggccgtca gcgaggtcgt cgttgacgac gagtacaccg ttacgatcaa gctcacgcgc
     1021 tccatctcgt tcctcccgtc actgctcgct ggcggttact tcggcttcga tagcccggcg
     1081 gccgtcatgg cggcgggcgc gaactacggc accccggccg tcggctccgt cggcacgggc
     1141 ccgtaccgct tcgagcgctg ggaagagggc agccgcgtgg tgctcaccgc gaaccccgag
     1201 taccgcgacg gcccgcccgc gaccgaaacg gtcgtcttcg taggcgtgag cgatccgacg
     1261 gcgcgtttgg cccagctcaa ggccggcgcc atcgacatcg ccgccgccat ctcgccgacc
     1321 gacttgggca gcgtgacgag cgaccccaac ctcgaactgg tccagagcgc gggtggcctt
     1381 aacacgagct acctcgcgat ccaccagaac cagaagccca tggatgacgt gcgcgtccgc
     1441 caggccgtcg cctacgcctt ggaccgcact gccatcgtcg acgccttcta cgccggtcag
     1501 gccgtagccg ccaacgacat cctccccgac tccctgtggg gccatgccga cggcgtcgat
     1561 gcctacccgt acaacccgga aaaggcgaag gaactcctcg ccgaagcggg ctaccccaat
     1621 gggtttgata ctgaattgtg gtaccgcaat tctggcctcg aacccgtgat cgcggaagcc
     1681 atcgcttcct acctggccga cgtcggcatc cgtgccgccc tcaagacgga ggactgggcc
     1741 gcgttcctcg ccgactacat ggccggtaac ttcgcgctct acacgctcgg ctggaacgcc
     1801 gacttcgccg acccggacac cttcatctac acgttctacg gcccacaggc cgtcaggcgc
     1861 ttcggctggg atgatcccgc cgtcgttgcg ctcacccagc aggcccggga ggttccgagc
     1921 caggacgagc gctctgagct ttacgctcag gtcatctctg ctgctcacga cgccatgccg
     1981 atgcttccgc tcgcgcacca gtcgcagttc ttcgtcgtcc gcaagggcgt taccggcctc
     2041 gtcctcaacc cgctcggcgc cctcccgacg ctcacgagca ccagcaaggc ccagtaactc
     2101 gctgggggag cccgcttgga gggctcccgg gtcaagacct acggccgcgc gcgtaaccaa
     2161 cgacgcgcgc ggccggtcgg acgcaaccgc taggggattc tgaccagtga cgacatatgt
     2221 gattcggcgc gtgctaggga tcatccccgt gcttctcggg gtcactttct tggtcttcgt
     2281 gatcaaccga gtcgtaccga gcaacccggc cgtggccatc cttggcgaca agggcaccgt
     2341 cgagtcgcag cgtcaactcg cagaggaact tgggctcaac cgacccatct ggttcgacgt
     2401 cgagggctac cgcgccaccg gcaagttcgg ccaactgttt gacagccaat atttcaagta
     2461 catgggtgag ttgctgacgg gcaacctagg caagtcgttc atgaccaggg cggccgttac
     2521 ggacagcctc aaggtgcgct tccccgcgac catggagctg gccatcttct cgatcatcat
     2581 cggcggcata ctcggcatag tccttggggt catcgccgcc ttgcggcgcg gcagttggat
     2641 cgataccggg atgctgttca tcgccgtttc gggcgtgtcg ttccccgtgt tctggaccg
//
LOCUS       DAOQYE010000182         2697 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_41286_length_2697_cov_2.622634, whole genome shotgun sequence.
ACCESSION   DAOQYE010000182 DAOQYE010000000
VERSION     DAOQYE010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41286_length_2697_cov_2.622634"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..720)
                     /locus_tag="PKN52_03980"
     CDS             complement(<1..720)
                     /locus_tag="PKN52_03980"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNQ99133.1"
                     /translation="MPLVKRRIGADHGGADQPPASFPFPRFRPGQGEALEQARAAFAA
                     GKRFVVIEAPTGAGKSAIAVTLAREAKSAFVITNQKILQDQYVRDFPDLALLKGRANY
                     ECLVAPTHAGAAPCTVGLRFPACDACPYFTAKDVAMAANVATLNYAYFLAELNYAGGF
                     APRELLVLDEAHNAEGALMGFVQVAFAEGALQRAGLPRALPPDLGDELAFEFAELLLP
                     EMRRRATEIERAMKDVADEDAA"
     gene            complement(948..1034)
                     /locus_tag="PKN52_03985"
     tRNA            complement(948..1034)
                     /locus_tag="PKN52_03985"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(998..1000),aa:Leu,seq:gag)
     gene            complement(1106..1990)
                     /locus_tag="PKN52_03990"
     CDS             complement(1106..1990)
                     /locus_tag="PKN52_03990"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HNQ99134.1"
                     /translation="MNELDFYREHSAMTDPGRSAAVWDAVNPTIEDMAAAVLPLVFHY
                     RGDGPFAENGIAEARIEEVNLRYAEDMLACIASHKPIEPGAVRLPHERMVGCCRDWAL
                     LLVSLARHHGYAARSRVGFASYFAAGWWIDHAVAEVWDEDQRRWRLVDAELRGPWVMA
                     SGQPVNPTDLSRDIFLPGPDAWRLVRAGELRAEAFVVAPEIPEPSLRGIPYLWHNVVQ
                     DLAALNRHEMVLWDVWGIDAEEVNAAGATLLDRVAAAESLEERRRFYSMDAFRVPATV
                     LSFRPYAPVPPVEVTLRA"
     gene            complement(1983..2561)
                     /locus_tag="PKN52_03995"
     CDS             complement(1983..2561)
                     /locus_tag="PKN52_03995"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNQ99135.1"
                     /translation="MTETTAVIEIRRASPADAPAIARVHVATWRETYAGLLPDDLLAN
                     LSVERREAQWAAQIAASGAPGDLNRVVVATLEGCGVVGFASCGPEREPESGFDAELYT
                     IYLLAEYHGRGIGRELFLRACEEAVAVGFRSVRLWVLEGNPTAGFYAHLGGEPVGRKG
                     LVLGDGSYFEAAFGWRDLLTMRGARIPHSNHE"
BASE COUNT          428 a          938 c          935 g          396 t
ORIGIN      
        1 ggcggcgtcc tcgtcggcca cgtccttcat ggctcgctcg atctcggtgg cgcggcggcg
       61 catctcgggc aggaggagct cggcgaactc gaacgccagc tcgtcgccca ggtcgggtgg
      121 gagggcgcgc ggcagaccgg cccgctgcag agcgccctcg gcgaaagcca cctgcacgaa
      181 gcccatcagc gcgccctcag cgttgtgggc ctcgtcgagg acgaggagct cgcgcggcgc
      241 gaagccgccc gcgtagttga gctcggcgag gaagtaggcg taattgagcg tggcgacgtt
      301 ggcggccatg gccacgtcct tggccgtgaa gtaggggcag gcgtcgcacg ccgggaagcg
      361 caggcccacc gtgcacggcg cggcgccggc gtgcgttggt gccaccaggc actcgtagtt
      421 ggcgcggccc ttgagcaggg cgaggtcggg gaagtcgcgc acgtactggt cctgcaggat
      481 cttctggttg gtgatcacga acgccgactt ggcctcgcgc gcgagcgtga ccgctatggc
      541 gctcttgccc gccccggtgg gcgcctcgat gaccacgaag cgcttaccgg cggcgaaggc
      601 cgcgcgcgcc tgctcgagcg cctcgccttg cccgggccgg aagcgcggga acgggaagct
      661 ggccggcggc tggtccgcgc cgccgtgatc ggctccgatg cggcgcttga ccaggggcat
      721 gggccaagct atcacgcgct cgcgcggcgg ctcggggtca ggtaggcgga agccgtgtcg
      781 cgcgtcccgg ttcacggccg gctctcggta aggcccggat ccagggcatc cggcagacgc
      841 ggtttgggcc ggaggaaggc ccaccgaccg ccgcggagtc gactcggtga acgaagtcgg
      901 gaccgactag tttcgtcggt cccgactaac ggcttgccgt cttctcgtgg tgccgaggag
      961 gggacttgaa cccctacagc cttgcggcca ccagcccctc aagctggcgc gtctacctat
     1021 tccgccacct cggcaagggt gccgtgaacg cgtgcaacac cgtccacaag gcgaaaatca
     1081 tgatatcgcc cacagaggcg gggcgtcaag cgcgcagtgt gacctcgacc ggcggcacgg
     1141 gagcgtacgg acggaagctc aacacggtcg ccgggacgcg gaaggcgtcc atggagtaga
     1201 accgccgccg ctcttcgagc gactcggcgg cggctacgcg gtccagaagc gtcgccccgg
     1261 cggcgttgac ctcttcagca tctatgcccc agacgtccca gagcaccatc tcatggcggt
     1321 tcagcgccgc caggtcctgc accacgttgt gccagaggta cgggatccct cgcaggctgg
     1381 gttcagggat ctctggagcg acgacgaacg cctcggcgcg cagctcgccg gcccggacca
     1441 gccgccaggc gtccggcccc ggcaggaaga tgtctcgcga caggtcggta gggttcacag
     1501 gctgcccgga agccatcacc cacgggccgc gcagctcggc gtcgaccagg cgccaccgcc
     1561 gctggtcctc gtcccagacc tcggccaccg cgtgatcgat ccaccagcca gcggcgaagt
     1621 agctggcgaa ccctacgcgg ctgcgcgcag cgtagccatg gtgcctggcc agcgacacca
     1681 gcagcagtgc ccaatcgcgg cagcatccga ccatgcgctc atgcgggagc cgaaccgccc
     1741 ctggctcgat cggcttgtgg gaggcgatgc aggccagcat gtcctcggca tagcgcaggt
     1801 tcacctcctc gatgcgcgct tcggcgatgc cgttctcggc gaacggaccg tcaccccggt
     1861 agtggaacac cagcggcagg accgcggccg ccatgtcctc gatcgtgggg ttcactgcgt
     1921 cccatacggc tgcggaccgg ccggggtccg tcatggccga gtgctcacgg tagaagtcga
     1981 gctcattcat ggttcgagtg tgggatccgc gcccctcgca tcgtcaagag gtctcgccaa
     2041 ccgaaggccg cctcgaagta cgagccgtca cccaggacga ggcccttccg gcctaccggc
     2101 tcgccgccga ggtgagcata gaagcccgcg gttggattgc cctccagcac ccagagccgc
     2161 accgaccgga accctaccgc cacggcctcc tcacaggcgc gcaagaagag ttccctgccg
     2221 atgccgcgac cgtggtactc ggccagcaag tagatggtgt agagctcggc gtcgaagccg
     2281 gactcgggct cgcgctcggg tccgcaagac gcgaagccga cgacgccaca gccctcgagg
     2341 gtggcgacga cgacacggtt gaggtcgccg ggcgcgccgg atgccgctat ctgagccgcc
     2401 cactgggcct cccgccgctc gaccgacagg ttcgccagca ggtcgtccgg caacagcccg
     2461 gcgtaggtct ctcgccacgt cgccacgtgc acacgggcga tggcgggcgc gtccgccggg
     2521 ctggcccgtc gtatctcgat cacggcagtg gtctccgtca tacgcccacc agcctatgcg
     2581 ggccggcctt cgcgagtggc tcaggtgtca tggcggtcgc tggtccgatc actccggtca
     2641 ctactagggt ctgttcactc ttgacggcac ggcgtcagaa gtcgtgcacg accgaga
//
LOCUS       DAOQYE010000183         2696 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_41305_length_2696_cov_2.477092, whole genome shotgun sequence.
ACCESSION   DAOQYE010000183 DAOQYE010000000
VERSION     DAOQYE010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41305_length_2696_cov_2.477092"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="PKN52_04000"
     CDS             <1..341
                     /locus_tag="PKN52_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99136.1"
                     /translation="MLALCSASAADPTVPAEFHSVLVTGGGFTANVGAEAYYLGGVTT
                     VFIHLAVAPATEYDSTAVGVFFPLSGQLLGGGGAAPECRDGRDNDGDGKTDYPSDPGC
                     ASPDDDSEAG"
     gene            402..1625
                     /locus_tag="PKN52_04005"
     CDS             402..1625
                     /locus_tag="PKN52_04005"
                     /inference="COORDINATES: protein motif:HMM:NF017984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HNQ99137.1"
                     /translation="MTEKLTAAEARRIFLAAQGLARKRSGSANRKRMRDYLNRQGVLQ
                     LDSVNVLARAHSLPLYTRHGAYDTAELDDYLWSSGETFEHWGHEASVMPRELLPALRY
                     RMEHWEERWQRHFEKVPRAGRWELIAAVERELHENGPLTSADLEHLEEEPRRNRGSWW
                     DWSATKDALEYLFMTGRAAVAGRPNFQRLYAAPAHVWGDLAHAPGLPKAQAQQELFDR
                     ALRADWIGTVGDLGDHFRIKTTLAKPLAVSAVERGLARWVKVEGWKEPALLSSEAQDP
                     GRATGAALLSPFDPACWYRDRLERMFGMEYRIEIYTPAPKRKYGYYTLPFLLGDQMAG
                     RVDLKAERKGGTLVVKAAWVEEAPAPGAKRRAPGAVAEALATELRTMAAWLGLSDVVV
                     EPVGTLAGELAATNG"
     gene            complement(1721..2443)
                     /locus_tag="PKN52_04010"
     CDS             complement(1721..2443)
                     /locus_tag="PKN52_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNQ99138.1"
                     /translation="MAASDDLGWVHRFVPAGAHVPDDSRGPGGAMAAASVLAPVAGPA
                     STDGLTLLLLHGTGGDETSLLELGRDVAPAANLLSVRGRSLEEGAPRFFRRFSATRYD
                     QAHLASEAVALADFVLAAEDRYGFANDELVALGYSNGANIAIATLGLRPGALAGAILL
                     RPAMPFESPPRADLTGVPVLVTRGRRDPYGPLSEPVAPYLRSLGATVAEEVLEAGHEL
                     TLRDLRTARAWLAEAPWRAGRP"
     gene            complement(2443..>2696)
                     /locus_tag="PKN52_04015"
     CDS             complement(2443..>2696)
                     /locus_tag="PKN52_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012009668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HNQ99139.1"
                     /translation="ERTVHRYMAMLDELGVPNSGPVDRFWFRSLYFREPGGVLFEIAT
                     DGPGFGVDEDLAALGDKVVLAPFLEPRRHEIVARLKALD"
BASE COUNT          405 a          959 c          982 g          350 t
ORIGIN      
        1 ccatgctggc gctctgcagc gccagcgccg ccgacccgac cgtgccggcg gagttccact
       61 ccgtgctggt gacgggcggg gggttcacgg ccaacgtcgg cgcggaggcc tactacctgg
      121 gcggggtgac gacggtgttc atccacttgg cggtcgcccc tgccaccgag tacgactcca
      181 ccgccgtcgg cgtgttcttc ccgctgtccg gccagctcct tggcggaggc ggcgcggcac
      241 cggagtgccg ggacggcagg gacaacgatg gggacggtaa gaccgactac ccgagcgacc
      301 caggctgcgc ctcgcccgac gacgactcgg aagcggggtg accagcgccg gacgttggcg
      361 tgtgagtcct tgctcaggca gcggcgcgtt agcctaggcg cgtgaccgag aagctgaccg
      421 cagccgaggc gaggcggata ttcctcgcgg cgcagggctt ggcgcggaag cgctcaggct
      481 cggccaaccg caagcgcatg cgcgactacc tgaaccgcca gggcgtcctg cagctcgaca
      541 gcgtcaacgt gctcgcacgc gcccactccc tccccctgta cacccggcac ggcgcctacg
      601 acacggccga actggacgac tacctatggt cgtccggcga gaccttcgag cattggggcc
      661 atgaggcgtc cgtcatgccg cgcgagctcc tgccagcgct ccgctaccgc atggagcact
      721 gggaggagcg ctggcagagg catttcgaga aggtgccgcg cgcaggccgc tgggagctga
      781 tcgcggcggt cgagcgcgag ctgcacgaga acgggcccct cactagcgcc gacctcgagc
      841 acctcgagga ggagccacgc cgcaaccgcg ggagctggtg ggactggagc gccaccaagg
      901 acgccctcga gtacctgttc atgacgggtc gggcggctgt ggccgggcgg ccgaacttcc
      961 agcgcctcta cgccgccccc gcgcacgtgt ggggagacct ggcccacgcg ccgggcctgc
     1021 ccaaggccca agcccagcag gagctcttcg accgcgcgct ccgcgccgac tggatcggta
     1081 ccgtcggcga cctcggcgat cacttccgca tcaagacgac cttggccaaa ccgctggccg
     1141 tgtcggcggt ggagcgtggc ctggcgcggt gggtgaaggt ggagggttgg aaggagccgg
     1201 cgctcctctc gtcggaggcg caggacccgg gccgcgcgac gggcgccgcg ctcctctccc
     1261 ccttcgaccc ggcctgctgg taccgcgacc ggctcgagcg catgttcggc atggagtacc
     1321 gcatcgagat ctacaccccg gcaccgaagc gcaagtacgg ctactacacg ctccccttcc
     1381 tgctcggcga tcagatggcc ggccgcgtcg acctcaaggc cgagcgcaag gggggaacgc
     1441 tcgtcgtgaa ggcggcctgg gtcgaggagg cgccggcgcc cggcgccaag cggcgcgccc
     1501 ccggggcggt cgccgaggcg ctggcgaccg agctgcggac gatggccgcg tggctgggcc
     1561 tctcggacgt cgtcgtcgag cccgtcggca cgctggcggg ggaactggcc gcgaccaacg
     1621 gttgagccgt ctaggaccgg ctcggtcagg gtccggcgag gtggggcaac gtcggtagga
     1681 ggttgaccgc tcaggcgctc gccgctcagg cgctcgccgc tcagggcctg ccggcccgcc
     1741 acggcgcctc cgccagccac gccctcgccg tccgtaggtc tcggagcgtg agctcgtggc
     1801 cggcctcgag gacttcctcc gctacggtgg ctccgaggct ccgcaggtag ggcgcgaccg
     1861 gctcggagag gggcccgtac ggatcgcgcc gcccccgcgt cacgagcacc ggaacgccgg
     1921 tcaggtcggc tcgcggcggc gactcgaaag gcatggcggg cctgagcagg atcgccccag
     1981 ccagcgctcc cgggcgcagg cccaaggtcg cgatggcgat gttcgccccg ttcgagtaac
     2041 ccaatgcgac gagttcgtcg ttcgcgaagc cgtaacggtc ctcggccgcc aggacgaagt
     2101 cggccagcgc cacggcctcc gaggcgaggt gcgcctggtc gtagcgtgtg gcgctgaacc
     2161 ggcggaagaa ccgcggggcg ccttcctcga gcgatcggcc gcgcacgctc agcaggttcg
     2221 ccgccggagc cacgtcgcga ccgagctcga gcagcgaggt ctcgtcgcca cccgtgccgt
     2281 gcaggagcag aagcgtaagg ccgtccgtcg aggcggggcc cgcgacaggc gcgagaacgc
     2341 tggccgccgc catggcgccg cccggcccgc ggctatcgtc cggcacgtga gcgccggccg
     2401 gaacgaagcg gtggacccag ccgaggtcgt ccgacgcggc catcagtcga gagccttcag
     2461 gcgggcgacg atctcgtggc gacggggttc gagaaatggg gccagcacga ccttgtcacc
     2521 gagggcggca aggtcctcgt cgacgccgaa gcccggaccg tccgtcgcga tctcgaacaa
     2581 cacgccgccc ggctccctga agtacaggct acggaaccag aaccggtcga ccggcccgct
     2641 gttggggacg cccagctcgt cgagcatggc catgtagcgg tgcaccgtgc gctccg
//
LOCUS       DAOQYE010000184         2692 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_41390_length_2692_cov_3.400455, whole genome shotgun sequence.
ACCESSION   DAOQYE010000184 DAOQYE010000000
VERSION     DAOQYE010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41390_length_2692_cov_3.400455"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2424
                     /locus_tag="PKN52_04020"
     CDS             <1..2424
                     /locus_tag="PKN52_04020"
                     /inference="COORDINATES: protein motif:HMM:NF039815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99140.1"
                     /translation="TQEFMVKAGVELGWKAGVDFGASGKFSVETGYTGQWSSSFTSES
                     ARETQNAYERSTQTDRELQVDESLTREVQGAAMRLTVSLRNLGDIAFNISDIQVSAFI
                     LDQNFPGRLVPIATLTPENEPAGGYNLGPLVPERGPLVFISDQVFPSLVEQLMRNPNG
                     LVFKISNFDVTDEFGRNFAFTSQDVNDRTATVVIDYGAADSDGDGEGDVTERLKVSTS
                     AGRPVFDANGDGLIDDDDRILFDDRGRQVGITLAEALEEVLGLDHYDEDATPTASLNP
                     VQLANSYSTRVVGGVERLWRIRTAARELGNPLKAWAVLTPEGIVDNTSEDVRSRVLGA
                     GEGITLAYVQDLDDDGLPASIEYMLSCSDTNVDTDGDGLTDRFEAFEGWRVSVIGKGD
                     YTGYSSCSRTDTDLDGLSDAEELTLGTDAQQRDTDKDGLTDREEVRGFTMRTRGGVLL
                     TGVITDPLNPDSDGDTLPDGAERALGTNPNVNDGDLVFDDDGDTLVNAQEDDGWTVTY
                     YPVSTTPYVQPTAVVLHVTSARDLADTDGDGLRDDVEFQLGTNPRLADTDGDGLTDLQ
                     EVQLGTDPLDADTDDDLLSDGVEVNTPRRIQVTGAAPYTVHSDPLVADVDLDNLVDGQ
                     EVTLGTDPTKYDTDGDGASDTVEAARMADADPNNNTDPLVRDQLLRITYEMQGVRTSF
                     NNICGEAGGGNAYVTGNFYYSLVGVTHGGSYSTGGKNLGTDYVSVRNDLLVLTAAQGA
                     TLQAYTTGLTRWDGAIGYTLQNFSQDYLTSDGLFDSIKQNVFRQTQASANPEDCVVNI
                     RFTITPINN"
     gene            complement(2527..2685)
                     /locus_tag="PKN52_04025"
     CDS             complement(2527..2685)
                     /locus_tag="PKN52_04025"
                     /inference="COORDINATES: protein motif:HMM:NF014027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HNQ99141.1"
                     /translation="MSDSTTSYGSYSGAVPNEKITWGKLGIATPKFIIESDATIVAPL
                     MFAMILGQ"
BASE COUNT          506 a          966 c          860 g          360 t
ORIGIN      
        1 acccaggagt tcatggtcaa ggccggagtc gagctcggtt ggaaggccgg cgtcgacttc
       61 ggcgcgtcgg gcaagttcag cgtcgagacg ggctacacgg gccagtggtc gtcgagcttc
      121 acgtccgagt cggcgcgcga gacgcagaac gcctacgagc gctcgaccca gacggaccgg
      181 gagctccagg tcgacgagtc acttacacgc gaggtgcagg gcgcggcgat gcgcctgacc
      241 gtcagccttc gcaacctggg cgatatcgcc ttcaacatct cagacatcca ggtctcggcc
      301 ttcatcctcg accagaactt cccaggccgg ctcgtgccca tcgccaccct cacgccggag
      361 aacgagccgg cgggcggcta caacctcgga ccgctggtac ccgagcgcgg acccctcgtg
      421 ttcatcagcg accaggtctt cccctcgctc gtcgagcagc tgatgcgcaa cccgaacggc
      481 ctcgtgttca agatctccaa cttcgacgtc acggacgagt tcggccgcaa cttcgcgttc
      541 acgtcccagg acgtcaacga ccgcacggct accgtggtga tcgactacgg tgcggccgac
      601 agcgacgggg acggcgaggg cgacgtgacg gagcgactca aggtgtcgac gagcgccggc
      661 cggcccgtct tcgacgccaa cggcgacggc ctcatcgacg acgatgaccg tatcctcttc
      721 gacgaccgcg gtcgccaggt cggcatcacc ctcgccgagg ccctcgagga ggtactcggg
      781 ctcgaccact acgacgagga cgccactccg acggccagcc tcaaccccgt gcagctcgcc
      841 aacagctact ccacgcgcgt cgtgggcggc gtcgagcggc tgtggcgcat ccgcaccgca
      901 gcgcgcgagc tcggcaaccc cctcaaggcc tgggcggtac tcacgccgga gggcatcgtc
      961 gacaacacgt cggaagacgt gcgcagccgc gtcctcggcg cgggcgaggg cataacgctg
     1021 gcctacgtac aggacctcga cgacgacggc ttgcccgcgt cgatcgagta catgctcagc
     1081 tgctccgaca ccaacgtcga cacggacggc gatggtctca cggaccgctt cgaagcgttc
     1141 gagggctggc gcgtgagcgt gatcggcaag ggcgactaca cgggctattc gagctgctcg
     1201 cggaccgaca cggacctcga cggcctgagc gacgccgagg agctgaccct cggtaccgac
     1261 gcccagcaac gcgacaccga caaggacggt ctcacggacc gcgaggaggt ccgcggcttc
     1321 acgatgcgca cgcggggcgg cgtcctgctc accggcgtga tcacggaccc gctcaacccc
     1381 gactctgacg gcgacaccct gccagacggc gccgagcgcg ccctgggcac caaccccaac
     1441 gtgaacgacg gcgacctcgt cttcgacgac gacggcgaca cgctcgtgaa cgcgcaggag
     1501 gacgacggct ggacggtcac gtactacccg gtgagcacca cgccgtacgt gcagcccacc
     1561 gcggtcgtct tgcacgtgac gtccgccagg gacctggccg acaccgacgg cgacgggctg
     1621 cgagacgatg tcgagttcca gctcggcacg aacccgcgac tggcggacac cgacggcgac
     1681 gggctgaccg acctccagga agtccagctc ggcaccgacc cgctcgacgc cgacacggac
     1741 gacgacctgc tcagcgacgg ggtggaggtc aacacgccgc ggcgcatcca ggtgacgggg
     1801 gcggcgccat acaccgtcca ctccgacccg ctcgtggccg acgtggacct cgacaacctc
     1861 gtcgacggtc aggaggtcac gctcggcacc gacccgacga agtacgacac cgacggcgac
     1921 ggcgcgagcg ataccgtgga agccgcccgc atggcggacg ccgacccgaa caacaacacc
     1981 gacccgctgg tgcgcgacca gctcctgcgg atcacctacg agatgcaagg cgtacgcacc
     2041 tcgttcaaca acatctgcgg cgaggcaggg ggcggcaacg catacgtcac cggcaacttc
     2101 tactacagcc tcgtgggcgt cacccacggc ggtagctaca gcacgggcgg caagaacctc
     2161 gggacggact acgtgtcggt ccgcaacgac ctgctggtgt tgaccgccgc ccaaggcgcc
     2221 acgctgcagg cctacaccac gggcctcacg cgctgggacg gcgccatcgg ctacacgctc
     2281 cagaacttca gccaggacta cctgacgagc gacggcctgt tcgactccat caagcagaac
     2341 gtgttccgtc agacgcaggc gtcggccaac ccggaggact gcgtggtgaa catccgcttc
     2401 accatcacgc cgatcaacaa ctgaccttcg ggctcgcgtg gtggcgccga ccagcggcgc
     2461 caccacgcat cgaggcggcc gtcccctgct ctccaggggc ggccgcttcc atgagggagc
     2521 gccagcctac tgccccagga tcatcgcgaa catgagcggc gccacgatgg tcgcgtcgga
     2581 ctcgatgatg aacttcggcg tagcgatccc cagcttgccc caagtgatct tctcgttggg
     2641 cacggcgccg gagtacgaac cgtagctcgt ggtcgagtcg ctcacctggc ag
//
LOCUS       DAOQYE010000185         2690 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_41429_length_2690_cov_3.826565, whole genome shotgun sequence.
ACCESSION   DAOQYE010000185 DAOQYE010000000
VERSION     DAOQYE010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41429_length_2690_cov_3.826565"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..743
                     /locus_tag="PKN52_04030"
     CDS             <1..743
                     /locus_tag="PKN52_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ99142.1"
                     /translation="PTARVWFAPWLGLLAAMVLAALVAGVHALVSIRYKADQIISGTA
                     INLMGLGIPAVVLAGLYHNTSISDPIRSRLPEISFFGIPGLSFNVLVYVAFLLVPVVW
                     FVVFRTPFGLRLRAVGEHPEAADSLGISVTRMRYTGVMLSGVLAGLGGAYLSIGNLNQ
                     FISEMSGGRGFIALAALIFGKWHPLGVLGATLLFGAFQATEVLLGGGKLLPPTVVQSL
                     PYILTMLVLAGFVGRSVAPKGIGKAWNE"
     gene            752..2290
                     /gene="malQ"
                     /locus_tag="PKN52_04035"
     CDS             752..2290
                     /gene="malQ"
                     /locus_tag="PKN52_04035"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HNQ99143.1"
                     /translation="MSRRSGVLLHPTSLPGPHGIGELGREAFAWLDFLESAGQRLWQV
                     MPLGPTGYGDSPYQCFSAFAGNHYLIDLESLERAGHLTAGELAGLRALPPDRVDFGVV
                     IPLKLTLLGTAAERFLATAGAAERAALAEFTRANEAWLSDYALFMALKEANGGKAWNE
                     WPTGVRSRAAAAMESARAELAPAIAKHTVWQYWFREQWDALHAYAAERDIAVLGDIPI
                     FVAHDSADTWARPDQFHLDATGGPTVVAGVPPDYFSATGQRWGNPLYRWDVMARDGYA
                     WWVERVRATLGLVDMLRIDHFRGFAAYWEIPASEPTAVKGRWVAGPGQALFDALRAAL
                     GELPLVAEDLGVITPDVDALRTANGLPGMKVLQFAFAGDADDPYLPHNYDADCVVYTG
                     THDNDTTAGWFASAPEVERDLVRRYLACDDAAVPWSFLRLAQASVARTAVATLQDVLG
                     LGGEARMNTPGVATGNWSWRFRWQDVPYWVAPQLRELATLFGRLPGRGPADTPYRQST
                     TGEA"
     gene            complement(2305..>2690)
                     /locus_tag="PKN52_04040"
     CDS             complement(2305..>2690)
                     /locus_tag="PKN52_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99144.1"
                     /translation="GSIALTPTIVLALIVSALLLSAFVSALLLGIAMFARSFKEAQSY
                     VAPLSFLFILPAVALQFKDLIGLSDSVFYVPVLNVLILMDNAVRGVGTGREALITWGV
                     MLVAVLLLLRFALRNFKRESVIFRS"
BASE COUNT          386 a          963 c          913 g          428 t
ORIGIN      
        1 accccaccgc cagggtatgg ttcgcgccct ggctcggcct gctcgccgcc atggtcctcg
       61 ccgcgctcgt ggcgggcgtg cacgcgctcg tgtccatacg ctacaaggcc gaccagatca
      121 tctcgggcac cgccatcaac ctcatgggcc tcggcatccc tgccgtcgtg ctcgcggggc
      181 tgtaccacaa cacgtcgatc agcgacccca tccgctcccg cctacccgag atctcgttct
      241 tcggcatccc cggcctgagc ttcaacgtgc tcgtctacgt ggccttcctg ctcgtgcccg
      301 tcgtctggtt cgtcgtgttc cgcacaccgt tcggcctgcg cctcagagcg gtcggcgagc
      361 acccggaggc ggccgactcg ctcggcatca gcgtcacgcg catgcgctac acgggcgtca
      421 tgctctccgg cgtcctggcc gggctgggcg gcgcctacct ttccatcggc aacctcaacc
      481 agttcatcag cgagatgtca ggcggccgcg gcttcatcgc gctcgcggcc ctcatcttcg
      541 gcaagtggca tccgctcggc gtcctcggcg ccacgctcct gttcggcgcg ttccaggcca
      601 ccgaggtcct cctcggcggc ggcaagctgc tgcccccgac ggtcgtgcag agcctgccct
      661 acatcctgac gatgctcgtc ctggccggct tcgtcgggcg ctcggtcgcc ccgaagggca
      721 tcggcaaggc gtggaacgag tgagcgctcc ggtgtcgcgg cgttccggcg tcctgttgca
      781 cccgacgtcg ctgcccggcc cgcacggcat cggcgagctt gggcgcgagg cgttcgcgtg
      841 gctcgacttc ctcgagtccg ccggccagcg gctctggcag gtcatgcccc tcgggccgac
      901 gggttacggc gatagcccgt accagtgttt cagcgcgttc gccggcaacc attacctcat
      961 cgacctggag agcctagagc gcgccgggca cctgacggct ggcgaactgg ccggtctgcg
     1021 cgcgttgccg ccggaccgcg tggacttcgg ggtggtcatc ccgctgaaac tcacgctcct
     1081 tggcacggcg gccgagcgct tcctcgccac ggcgggtgcc gccgagcgcg ccgccctggc
     1141 cgagttcacg cgcgcgaacg aggcgtggct gtccgattac gccctcttca tggccctcaa
     1201 ggaggcgaac gggggcaagg cgtggaacga gtggcccacg ggcgtgcgca gccgcgccgc
     1261 tgccgctatg gagtccgcgc gcgccgagct ggcgcccgcc atcgccaagc acaccgtgtg
     1321 gcagtactgg ttccgcgagc aatgggacgc gctgcacgcc tacgctgccg agcgcgatat
     1381 cgccgtcctc ggcgacatcc cgatcttcgt cgcgcacgat tcggccgaca cgtgggcgcg
     1441 ccccgaccag ttccatctcg atgccaccgg cggtccgacc gtcgtggccg gcgtgccgcc
     1501 cgactacttc tcggccacgg gccagcgctg gggcaacccc ctgtaccgct gggacgtgat
     1561 ggcaagggac ggctacgcct ggtgggtgga acgcgtgcgc gcgacccttg gccttgtcga
     1621 catgctgcgc atcgaccact tccgcggctt cgccgcctac tgggagatcc cggcgtcgga
     1681 gccgactgcc gtgaagggcc ggtgggtcgc ggggcccggc caggcgctct tcgacgccct
     1741 gcgagctgcc ctcggtgagc tgccactggt ggccgaggac ctgggcgtca tcacgcctga
     1801 tgtcgacgcg ctgcgcaccg ccaacggcct gccaggcatg aaggtcctgc agttcgcctt
     1861 cgccggcgac gcggacgacc cgtacctgcc tcacaactac gacgccgact gtgtcgtgta
     1921 cacggggacg cacgacaacg acacgacggc gggctggttc gcctccgccc cggaggtcga
     1981 gcgggacctc gtcaggcgct acctggcatg cgacgacgcg gccgtgccgt ggtcgttcct
     2041 gcggctggcc caggcctcgg tcgcgaggac ggcggtcgcg acgttgcagg acgtcctcgg
     2101 cctaggtggc gaggcgcgca tgaacacgcc tggggtcgcg accggcaact ggagttggcg
     2161 gttccgatgg caggacgtgc cctactgggt ggcaccgcaa ctccgcgaac tggcgaccct
     2221 gttcgggcgc ttgccaggcc gcgggccggc cgacacgccg taccgccagt cgaccaccgg
     2281 cgaggcctga gcggctctcg aggtctagct cctgaagatg acggactcgc gcttgaagtt
     2341 gcgcaaggcg aaacgtagca ggaggaggac ggccaccagc atcacgcccc acgtgatgag
     2401 ggcttcccga ccggtgccga ctccccgcac cgcgttgtcc atcaggatca ggacgttgag
     2461 aacgggtacg tagaacaccg agtcggacag gcctatcagg tccttgaact gcagtgcgac
     2521 ggcggggagg atgaacagga acgagagcgg cgccacgtag ctctgcgcct ccttgaacga
     2581 gcgcgcgaac atcgctatgc cgaggagcag cgccgagacg aacgcggaga ggagcagggc
     2641 gctcacgatg agcgcgagga cgatggtcgg cgtcagggcg atgctgccac
//
LOCUS       DAOQYE010000186         2689 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_41471_length_2689_cov_3.092255, whole genome shotgun sequence.
ACCESSION   DAOQYE010000186 DAOQYE010000000
VERSION     DAOQYE010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41471_length_2689_cov_3.092255"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="PKN52_04045"
     CDS             complement(<1..448)
                     /locus_tag="PKN52_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99145.1"
                     /translation="MSTAPKTVLGIPRVEAPWLLYLCFLAFQPLFDPDSGIGDWLVIA
                     ASVAVFLPVYGFTHRVIGRRQFFRRAGVPGALIGIVAMLALGVLTTLFNSAGNVFIIY
                     AAAAAGKLHRAAAGAGRRAGAARAARAAGAFRLARREPDARTGRSAQ"
     gene            complement(473..1297)
                     /locus_tag="PKN52_04050"
     CDS             complement(473..1297)
                     /locus_tag="PKN52_04050"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ99146.1"
                     /translation="MTTSTPAGMTKAASDAVQAPRRNLPRLYLTEARFEFLKLLRLPG
                     YSVSVLAFPVMFYALFGALFGDYAVQGISTAKYMVVGYSAAGVLAASLFGFGVGVASE
                     RGQGWLRLKRVSPMPPLAYFFAKVVMALLFSTLVVLVLTLLGAVLQGVRFEAGTWLRM
                     YGALMLGVLPFASLGLAFGYLFGPNSAPMVLNLIYTPAAFASGLWIPLTVMPPLLQKI
                     AEFLPTYHFAQFALSQAGAEPVGSGLTHVLVLAITTVVCLGLAVWAYFRDEGKTYG"
     gene            complement(1294..2331)
                     /locus_tag="PKN52_04055"
     CDS             complement(1294..2331)
                     /locus_tag="PKN52_04055"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ99147.1"
                     /translation="MTDTRAAIMDRSPATDPAEPVAELVEATKRYGAVTALDRVSLRA
                     MPGEVLAVLGPNGAGKTTAVSLMLGLARPTSGRARLFGADPRSAGSRMRIGTMLQVSG
                     VPATLTVREHLTAFATYYPAPLGVDEAIAIAGLEGVANRLYGKLSGGQQQRLHFALAI
                     VGDPDALFLDEPTTGLDAASRRAFWNHVREYLGGRRSVILTTHYLEEADALADKVVVL
                     DHGKVVAYGTPTEVKARAAGRKVRAVTRLSTAEVSALAGVRSVERSGAVTTILSTDSD
                     ATVRALLAADPRMSGLEVTGAGLEEAFLSLTSGTGGDDGEGNAAAGPRDRGGGRDGGR
                     DSYAATGRTEP"
BASE COUNT          441 a          903 c          970 g          375 t
ORIGIN      
        1 cttgcgcgct tcggccagtt cgtgcatccg gttcacgccg ggccagccga aatgcgcctg
       61 ccgctcgagc cgcgcgtgcc gcccccgctc gtcgtcctgc gccagctgca gcgcgatgaa
      121 gcttcccggc cgcggcggcg gcgtagatga tgaacacgtt gccggcgctg ttgaagagcg
      181 ttgtgagcac gccgagcgcc agcatggcga ctatcccgat cagggccccc ggcacaccgg
      241 cgcggcggaa gaactggcgc cggccgatga cgcggtgggt gaagccgtag accggcagga
      301 agaccgccac cgaggccgct atcacgagcc agtcaccgat ccccgagtcc gggtcgaaga
      361 ggggctggaa ggccaggaag cagaggtaga ggagccacgg ggcctcgacc ctcgggatgc
      421 cgagtaccgt cttgggggcg gtgctcatgg ctgccaggat aaggtcggag ccttagccgt
      481 acgtcttccc ctcgtcgcgg aagtatgccc acacggccag accgaggcag acgaccgtgg
      541 tgatcgccag cacgagcacg tgcgtcagac ccgacccgac cggctcggcg ccggcctggg
      601 agagggcgaa ctgggcgaaa tggtaggtcg gcaggaactc cgcgatcttc tgcagcaggg
      661 gcggcatgac cgtgagcggg atccagaggc ccgaggcgaa cgcggccggg gtgtagatca
      721 ggttgaggac catcggcgcg ctgttagggc cgaagaggta gccgaacgct aggccgagcg
      781 aggcgaacgg caggacgccg agcatgaggg cgccgtacat ccggagccac gtccccgcct
      841 cgaagcgcac gccttgcagc acggcgccga ggagcgtgag caccaggacg acgagcgtgc
      901 tgaagagcag ggccatgacg accttcgcga agaagtacgc gagcggaggc atgggcgaga
      961 cgcgcttgag gcgcagccag ccctgcccgc gctcggaggc cacgccaacg ccgaagccga
     1021 acagcgaggc cgccagcacg cccgccgcgc tgtagccgac gaccatgtac ttggcggtgc
     1081 tgatcccctg cacggcgtag tcgccgaaca gcgcgccgaa gagcgcgtag aacatgacgg
     1141 ggaaggcgag gaccgagacg gagtacccgg gcagtcgcag gagcttcagg aactcgaagc
     1201 gcgcctcggt caggtagagc cggggcaggt tgcggcgagg cgcctgaacg gcgtcggaag
     1261 cggccttggt catgccggcc ggagtggagg tggtcatggt tcggtccttc ccgtggcagc
     1321 gtagctatcc cggccgccat cccggccacc gcccctatcc ctcggtccgg cggcggcgtt
     1381 cccttcgccg tcatcgccgc cggtgccgct cgtcaggctg aggaacgcct cctcgagccc
     1441 ggcgccggtc acctccagtc cgctcatccg cgggtcggcg gcgaggagcg cgcgcacggt
     1501 ggcgtcggag tcggtggaga gtatggtggt caccgcgccg gagcgctcga cgctcctaac
     1561 cccggcgagc gccgacacct cggcggtgga caggcgcgtc acggccctga ccttgcggcc
     1621 ggcggctcgc gccttgacct ccgtcggcgt gccgtacgcc acgaccttgc cgtgatcgag
     1681 aacgacgacc ttgtcggcga gcgcgtcggc ctcctcgagg tagtgggtgg tcaggatcac
     1741 gctgcggcgg ccgccgaggt actccctgac gtggttccag aacgcgcggc gcgaggcggc
     1801 gtccaacccg gtggtcggtt cgtcgaggaa gagcgcgtcc ggatcgccga cgatggcgag
     1861 cgcgaagtgc aggcgctgct gctggccgcc ggagagctta ccgtagagcc ggttggcgac
     1921 gccctcgaga cctgcgatgg cgatggcctc gtcgacgcca agcggcgctg ggtagtaagt
     1981 cgcgaacgcg gtcaggtgct cgcgcacggt gagcgtcgcg ggaaccccgg acacttggag
     2041 catcgtgcct atgcgcatgc ggctaccggc cgagcgcggg tcggccccga agaggcgcgc
     2101 ccgtccggac gtcggcctcg ccaggccgag catcagggag acggccgtgg tcttccccgc
     2161 cccgttcggt ccgaggacgg cgagcacctc gcccggcatg gcacgcaggc tcacgcggtc
     2221 gagcgccgtc acggcgccgt aacgtttcgt cgcttccacc agttcggcga ccggctccgc
     2281 cgggtcggtc gcgggggagc ggtccatgat ggctgcccgt gtgtctgtca tgcctcaagg
     2341 gtgctcccgt cggcgcgccg gcggcagtgt cggtggtcag ggccgagaga tgacaggtgt
     2401 cacaagccga ggacgaccca cgcctaggta caccctggta aaaccctggt acaccactgg
     2461 taaaccccta taaccgtcat ataaccgcgc ggcctctaac gttcggccaa tcacccggaa
     2521 gtcgggcgtc tcatgcgcgc ctgtacgccg aacgcgttgc gccccgcaca cgccaccggc
     2581 ggccctccga gatccgagtc agttaggagc ggtcatgaag ctacgagcgt ttgcccaagc
     2641 actcttcccg gcggtggtta ccttcctggt cgcctgcggt ggtacgccc
//
LOCUS       DAOQYE010000187         2688 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_41488_length_2688_cov_3.556400, whole genome shotgun sequence.
ACCESSION   DAOQYE010000187 DAOQYE010000000
VERSION     DAOQYE010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41488_length_2688_cov_3.556400"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="PKN52_04060"
     CDS             complement(<1..561)
                     /locus_tag="PKN52_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase domain-containing
                     protein"
                     /protein_id="HNQ99148.1"
                     /translation="MSAHRPVRIAYQGVSGAFSEEAALGFAPGGTAVGFPSFEEAFAA
                     AADGTCDYACLPVENSIAGSINQTYDLLTDSALNVVGERIVRVHHNLLAKPGVKLEDV
                     KRVYSHPQALAQCAGFIRRYKLEAVTDFDTAGAAMLLAQNGGDGKAAIASVRAAQEYG
                     LEVLAEKIEDLPFNFTRFFVLSYGEGN"
     gene            complement(626..2353)
                     /locus_tag="PKN52_04065"
     CDS             complement(626..2353)
                     /locus_tag="PKN52_04065"
                     /inference="COORDINATES: protein motif:HMM:NF012239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HNQ99149.1"
                     /translation="MLELPTRTRDLITKERGAITDLLVLLAKLEAEPDHVADVRTAVD
                     DLDGIFLLVVAGEFNAGKSSLVNALLGERVMPEGVTPTTDRVTVITHGDEIGEVMDGR
                     DVVRRTYPSELLDTVAFVDTPGTNAIVASHQALTERFVPRADLVLFVTSADRPFTQSE
                     REFLELIASWGKKVVMVVNKVDILASDSERETVLEYVAHHARDTLGSTPEVFGVSARQ
                     AFQARQVGDPAGLAASGLPELEASIRARLGADRLKLKLLTPLGVAKRTSEHYKEVLDN
                     RLGLLTDDSTTLEVIDRQRKRFESEMRRDLGLHVGAIARVLEDVARRGEEFFDDTIRW
                     RRFVKLMNTERVKEEFQAKVIKGAEGEIDARLGELVDWFIQRNLQFWEDVMRFLNEKR
                     AAEQERVIGEVGGRFQYDRQALLRGLRERAEGALEEYDAPGEAKAIADRLQASVLRTG
                     LLEVGGIGLSAAMLALLTGAAFDITGIAIGLTAIGLGLLVFPRQRARSKRELRTKLDE
                     LRTALEEGVGKQFALELERSQEKLAGAISPYTRFVGAELERLHELQAELGELTGRLAA
                     LKHEVETTA"
     gene            2423..>2688
                     /locus_tag="PKN52_04070"
     CDS             2423..>2688
                     /locus_tag="PKN52_04070"
                     /inference="COORDINATES: protein motif:HMM:NF014165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99150.1"
                     /translation="MSEALRAFRGEVAILSDLAAAADLLSWDQDTYMPPGASEGRGEQ
                     EATLHAIRHARLTDPAYGDLLEELEGSGLDPAGSDHAMVREARRT"
BASE COUNT          396 a          904 c          934 g          454 t
ORIGIN      
        1 gttgccctcg ccgtaggaga ggacgaagaa gcgcgtgaag ttgaagggga ggtcctcgat
       61 cttctcggcc agtacctcga gcccgtactc ctgcgcggca cgaacgctgg cgatggccgc
      121 cttgccatcg ccgccgttct gcgccaggag catcgcggcg cccgccgtgt cgaagtccgt
      181 gaccgcctcc agcttgtatc gccggatgaa cccggcgcat tgggcgagcg cctgcgggtg
      241 gctgtagacg cgtttcacgt cctcgagctt gacgccgggc ttggcgagca ggttgtggtg
      301 aacgcgcacg atgcgctcgc ctacgacgtt gagggccgag tcggtgagca ggtcgtacgt
      361 ctggttgatc gagccggcga tgctgttctc gacgggtagg caggcgtagt cgcacgttcc
      421 gtcggcggcc gcggcgaacg cctcctcgaa ggaggggaac ccgacggcgg tgccgccggg
      481 ggcgaaccct agggcggctt cctcgctgaa agctccggag actccttggt aggcgatgcg
      541 aacgggccgg tgtgcgctca tgcggaggag tgtaacccta cgccgcgcgt gccgactgga
      601 ggcgcgttcg gggggcgtcg gtcgctcagg cggtcgtttc gacctcgtgc ttcagcgccg
      661 cgaggcgtcc ggtcagctcc ccgagctcgg cttgcagttc gtgcagccgc tccagctcgg
      721 cgccgacgaa gcgcgtgtac gggctgatgg cgccggccag cttctcttgc gagcgttcga
      781 gctcgagggc gaactgcttg ccgacgccct cctcgagggc ggtcctcagc tcgtcgagct
      841 tggtcctcag ctcgcgcttc gagcgggctc gttggcgcgg gaacaccaac agtccgaggc
      901 cgatggcggt gagaccgatc gcgatgccgg tgatgtcgaa ggccgctccg gtaaggagcg
      961 cgagcatggc cgcgctcaac ccgatgcccc cgacctcgag gagcccggtc ctgagaaccg
     1021 acgcttggag gcggtcggcg atcgccttgg cctcgcccgg cgcgtcgtac tcctccagcg
     1081 cgccctcggc gcgctcacgc aggccacgga gcagcgcctg cctgtcgtac tggaaacgcc
     1141 cgccgacctc gccgatgacc cgctcctgct ccgcggcgcg cttctcgttg aggaagcgca
     1201 tgacgtcctc ccagaactgg aggttgcgct ggatgaacca gtcgacgagc tcaccgaggc
     1261 gcgcgtcgat ctcgccctct gcgcccttga tgaccttggc ctggaactcc tccttgacgc
     1321 gctccgtgtt catgagcttg acgaagcggc gccagcggat ggtgtcgtcg aagaactcct
     1381 cgccgcgcct ggcgacgtcc tcgagcacgc gcgctatggc gccgacgtgc aggccaaggt
     1441 cgcgccgcat ctccgactcg aagcgcttgc gctgtctgtc gatcacctcg agcgtcgtgc
     1501 tgtcgtcggt gaggaggccg agccggttgt caaggacctc cttgtaatgc tccgacgtgc
     1561 gcttggcgac gccgagcggc gtgagcagct tcagcttgag acgatcggca ccgaggcgcg
     1621 ccctgatgga cgcctccaac tcgggcaagc cgctcgcggc gaggccggcc ggatcgccta
     1681 cctgccgggc ctggaacgcc tggcgggcgc tgaccccgaa cacctccggc gtcgagccga
     1741 gcgtgtcgcg cgcatggtgc gcgacgtact cgaggaccgt ctcccgctcg gagtccgagg
     1801 cgaggatgtc caccttgttg acgaccatca cgaccttctt gccccaagag gcgatgagtt
     1861 ctaggaactc ccgttcgctc tgggtgaagg ggcggtcggc gctagtgacg aagagcacga
     1921 gatcggctct cggcacgaac cgctccgtca acgcctggtg ggaggccacg atggcgttcg
     1981 taccgggcgt gtcgacgaag gctaccgtgt cgagcagctc gctcgggtag gtgcggcgga
     2041 cgacgtcgcg cccgtccatc acctcgccga tctcgtcgcc atgcgtgatg acggtcacgc
     2101 ggtccgtcgt gggcgtgacg ccttccggca tcacgcgctc gcccaggagc gcgttgacca
     2161 agctcgactt gccggcgttg aactcgccgg cgaccaccag caagaagatg ccgtccagat
     2221 cgtcgacggc cgtgcggacg tcggcgacat ggtccggctc ggcctcgagc ttggccagca
     2281 gcacgaggag gtcggtgatc gccccccgct ccttggtgat gaggtcccgc gtcctggtgg
     2341 gcaactcgag catcgaggcg aagataccac gctggcatcg gctttcctgc ccccggcgct
     2401 ggtattgtct ggcatgcgag acgtgtctga agcactacgt gcctttcgcg gcgaggtcgc
     2461 catcctgagc gatctggccg ccgcggctga ccttctgtcc tgggatcagg acacctacat
     2521 gccgccgggt gcctcggagg gccgaggcga gcaggaggcc accctgcatg ccatacgcca
     2581 tgcgcggctg accgacccgg cctacggcga cctcctcgag gagctcgagg ggagcggtct
     2641 cgacccggcc ggaagcgacc acgccatggt gcgagaggcg cgccgcac
//
LOCUS       DAOQYE010000188         2688 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_41498_length_2688_cov_2.768705, whole genome shotgun sequence.
ACCESSION   DAOQYE010000188 DAOQYE010000000
VERSION     DAOQYE010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41498_length_2688_cov_2.768705"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..874)
                     /locus_tag="PKN52_04075"
     CDS             complement(<1..874)
                     /locus_tag="PKN52_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNQ99151.1"
                     /translation="MPSSKVETPAGTMSRSRTAEILAAAETHGSGLWHPDVVFVRGAG
                     ALIYDADGREYLDCLAGIAVASVGHANERLADALARQARRLIVCPQNLGNDVRTEFVD
                     KLFQFVGPPLTRAFLGNSGAEANEAALKWARVATGRRRFVAAQRGFSGRTMGVLPLTW
                     EPKYREPFAPYDTEVEFVPFNDVVALEAAVDDRTAAVLLEPIQGESGIRPATQEFLAA
                     ARAATKRSGALLVLDEIQTGVGRTGTFLASEAFDIQPDMVTLAKGLAGGVPISALLMT
                     DEVAGAMPKGGHGTT"
     gene            complement(976..1689)
                     /locus_tag="PKN52_04080"
     CDS             complement(976..1689)
                     /locus_tag="PKN52_04080"
                     /inference="COORDINATES: protein motif:HMM:TIGR03605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SagB family peptide dehydrogenase"
                     /protein_id="HNQ99152.1"
                     /translation="MTASKGRTFGAEFFDQTKYRRGQLPPSRTRVAPPFKVLANPSEV
                     ASLPAPQLKGGTGIWTALSRERSDVAEGGRLKQTHLSQLLWGSAGFNYGRQRVHVSAQ
                     AASSLEAYLLVLNVQDLFAGVYHYNPRDHSLDQLRMGEVASEFAEVVLAPIETAAQAA
                     VVCFTGVPGRHRVADGGRPYRYLMLDAGAAAQGLVLTASALGLAATYAADFYDDELAK
                     LLNVDGRTEVPLCFVTIGS"
     gene            1800..2201
                     /locus_tag="PKN52_04085"
     CDS             1800..2201
                     /locus_tag="PKN52_04085"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNQ99153.1"
                     /translation="MELRVRFVETDQMGVVHHSAYVPWFEAGRVEWLRERGLSYREME
                     DSGLSLAVASVAVDYRSAAYFDDLLEVTSTLTEVRSRRVAFAYRVARPNDGALVATAT
                     TTHVPTDRSGHAVRLPGRWLAPLLALLATDP"
     gene            2245..>2688
                     /locus_tag="PKN52_04090"
     CDS             2245..>2688
                     /locus_tag="PKN52_04090"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HNQ99154.1"
                     /translation="MNRQAVREAPAKNPVEKIVGTALTFDDVLLVPAHSRVLPKDVDL
                     SARLTGSVRLNLPIVSAAMDTVTETQMAIAMARLGGIGVIHKKMSVEAQAEMVRKVKR
                     SESGMIVDPITLERHATLADAERLMGEYSISGVPIVERDGKLVGIL"
BASE COUNT          428 a          936 c          933 g          391 t
ORIGIN      
        1 aggtcgtgcc gtgaccgccc ttcggcatgg cgcccgccac ctcgtccgtc atgagcagag
       61 cgctgatcgg cacgccgccc gccaagccct tggcgagcgt gaccatgtcc ggctggatat
      121 cgaacgcctc gctagccagg aacgtgcccg tgcgaccgac gcccgtctgg atctcatcga
      181 gcacgaggag cgccccgcta cgcttcgtgg cggcgcgggc ggcggccagg aactcctgcg
      241 tcgcaggcct gatcccgctc tctccctgga tcggctcgag aagcacggcg gccgtgcggt
      301 cgtcgacggc cgcctccaag gcgaccacgt cgttgaaggg cacgaactcg acctcggtgt
      361 cgtacggtgc gaacggttcg cggtacttcg gctcccacgt gaggggcagc acgcccatgg
      421 tgcgacccga gaacccgcgc tgggcagcca cgaagcgcct tcggccagtg gccacgcgcg
      481 cccacttcaa agccgcctcg ttggcctcag cccccgagtt gccgaggaaa gcgcgcgtga
      541 gcggcggccc gacgaactgg aacagcttgt cgacgaactc ggtgcggacg tcgttgccga
      601 ggttctgcgg gcagacgatc aaccgccgag cctgccgcgc gagagcgtcg gcgaggcgtt
      661 cgttggcgtg accgacgctc gccaccgcga tgccggccag acagtcgagg tactcgcgac
      721 cgtccgcgtc gtagatgaga gcgccggcgc cgcgcacgaa cacgacgtcg gggtgccaca
      781 ggccgctgcc gtgggtctct gccgcggcga ggatctcggc ggttcgggaa cggctcatgg
      841 tgccggcagg cgtttcgact ttggaactcg gcatgcgttg tgctccctct cggcgcggcg
      901 gctcaccgaa cgaccgcgtg tccgggaagc ataacgccga cgcaccaccg tacgcgcctc
      961 gcaccggggc accgttcagg aaccgatggt cacgaaacag agcggcactt cggtgcgccc
     1021 gtcgacgttg agcagcttgg cgagctcgtc gtcgtagaag tcggcggcgt aggtcgccgc
     1081 caagccgagg gcgctggcgg tcagcacgag gccctgcgcg gccgcaccgg catcgagcat
     1141 gaggtagcgg tagggtcggc cgccgtcagc gacccggtga cggcccggca cgccggtgaa
     1201 acagacgacc gcggcctgcg ccgccgtctc gatgggcgcc agcaccacct ccgcgaactc
     1261 gctcgccact tcacccatcc tgagctgatc gaggctgtgg tcgcgcgggt tgtagtggta
     1321 gacgcccgcg aagaggtcct gaacgttgag aacgagcaga taggcctcga gggatgaggc
     1381 cgcctgcgcg ctcacgtgca cccgctggcg accgtagttg aagccggccg agccccagag
     1441 gagctgcgat aggtgggtct gcttcaaccg accgccctcg gccacgtcgc tgcgttcgcg
     1501 cgacagggcg gtccagatgc ccgtcccgcc cttcagttgc ggagccggca agctcgcgac
     1561 ctcgctgggg ttggcgagca ccttgaacgg cggcgccacc cgagtgcgcg acggcggcaa
     1621 ctgcccgcgg cggtacttcg tctggtcgaa gaactccgca ccgaacgtgc ggcctttgct
     1681 ggctgtcact agtctggctc ccgcaggcac tgtagcaccg cttagcacgc gtgtagcggg
     1741 gcgcggggtt atgctgccgt agcgcccggc cgccaagcgc gcgcggcgtc catagcagca
     1801 tggaactgag agtcaggttc gtcgagacag accagatggg cgttgtccac cacagcgcct
     1861 acgtcccatg gttcgaggca gggcgcgtcg agtggctgcg ggagcgcggc ctcagctacc
     1921 gcgagatgga agactcgggc ctgtccctcg ccgtcgcgag cgtcgcggtc gactaccgct
     1981 cggcggcgta cttcgacgac ctcctcgagg tcacgagcac cctcacggag gtgcgcagcc
     2041 ggcgcgtcgc gttcgcctac cgggtcgcgc gaccgaacga cggcgcgctc gtcgctacgg
     2101 ccaccaccac ccacgtgccg acggatcgct cggggcacgc cgtgcgactc ccagggcggt
     2161 ggctggcgcc gctgctcgcg ctgctcgcca ccgacccgtg aaccaccagg ccgtcacccc
     2221 cggtcgcgaa ggagaaggca ccgcatgaat cgacaagctg tccgagaggc cccggccaag
     2281 aaccctgtcg agaagatcgt cggcaccgcg ctgaccttcg acgacgtgct cttggtgccg
     2341 gcgcacagcc gagtgctccc gaaggacgtc gacctctcgg cgcgcctgac gggctccgtg
     2401 cgcctcaacc tgccgatcgt gtcggcggcc atggacaccg tgacggagac gcagatggcg
     2461 atcgccatgg cgcggctcgg cgggatcggc gtcatccaca agaagatgtc ggtggaggcg
     2521 caggccgaga tggtgcgcaa ggtcaagcgc tccgagtcgg gcatgatcgt cgacccgatc
     2581 accctcgagc ggcacgccac ccttgccgac gcggagcgcc tgatgggcga gtactcgatc
     2641 agcggggtgc ccatcgtgga gcgcgacggc aaactcgtcg gcatcctg
//
LOCUS       DAOQYE010000189         2679 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_41655_length_2679_cov_3.510290, whole genome shotgun sequence.
ACCESSION   DAOQYE010000189 DAOQYE010000000
VERSION     DAOQYE010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41655_length_2679_cov_3.510290"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..741
                     /locus_tag="PKN52_04095"
     CDS             <1..741
                     /locus_tag="PKN52_04095"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ99155.1"
                     /translation="SFMSRGPGGQPVPPATLAQYERTFTVFGPVLWRSIGVALLATVI
                     SLVLGYPLAFFISRRRLPATRMFLLFLVMLPFWTNFLVRTYALITIMQRDGLLNGLFQ
                     SLGIITAPLQMLFTPAAVMVGLVYGYLPFMILPIYASVEALDRRYVEAANDLGANDWT
                     AFWRIIFPLTLPGVIAGSILVFIPAIGAYVTPDLMGGTRGLMIGNLIQGQFGGRGNVP
                     LGSAISMVLLAMVFISVLVYTRWGQREE"
     gene            741..1664
                     /locus_tag="PKN52_04100"
     CDS             741..1664
                     /locus_tag="PKN52_04100"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ99156.1"
                     /translation="MVQPRPAPDQKTVARKVRRDMAVRRTGRFGMWLSPFVVYLFLWV
                     PIIVLVVFSFNDGNSVSVWRGFTTRWYENILHGTISAGTEAARFQTELLLKALRNSLL
                     VATVATVISSALGTMLSLAIARGRFWGRKTLDTLFYLPVVIPDITQGIALAVFFNIVF
                     ETVQRLFGVRLVSGFGTIVIAHVAFNISYVTIVVRARLAGMDPRLEEAGRDLGANHWQ
                     TFWRITFPNLLPGVMAGALLAFTLSLDDFVITFFNSGVGTTTLPLFVYGMLKQRVPPE
                     INAISTLMILASIVLVGGSLLLQQRGNRDKA"
     gene            1672..>2679
                     /locus_tag="PKN52_04105"
     CDS             1672..>2679
                     /locus_tag="PKN52_04105"
                     /inference="COORDINATES: protein motif:HMM:NF014594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HNQ99157.1"
                     /translation="MRRVSAAEAVNVVRSGDRVFIHSVAAAPQALIAALTARAAELRD
                     VEIVQLHTEGPAPYAAPEMAGSFRVNALFVGKNVRDAVARGQADYVPVFLSEVPALFR
                     NRAMPLDVALISVSPPDKHGFCSLGVSVDASRAAAQSARTVVAQVNAAMPRSHGDGLI
                     HVDDIDLAVEEAAPIFEVSQAKTGELERTIGGFVASLVEDGATLQLGIGAIPEAVLAA
                     LTDHHDLGVHTEMFSDGIVDLVERGVVTGALKRNHPGKVVASFVMGSRRLYDFIDDNP
                     SVLLLDVAYTNDTAVIRRNPKVTAVNSALEIDLTGQVCADSLGTFQYSGVGGQMDFIR
                     GA"
BASE COUNT          371 a         1015 c          823 g          470 t
ORIGIN      
        1 tccttcatgt cgcgcggccc gggcgggcag cccgtgccgc ccgccaccct cgcgcagtac
       61 gagcgcacgt tcacggtgtt cgggcccgtg ctgtggcgct ccatcggggt agcgctcctg
      121 gccaccgtca tcagcctcgt tctcggctac cccctcgcgt tcttcatcag ccggcgccgc
      181 ttacccgcca cgcgcatgtt cttgctattc ctcgtcatgc tgccgttctg gacgaacttc
      241 ctcgtgcgca cctacgcgct catcacgatc atgcagcgcg acggcctcct caacggcctc
      301 ttccagagcc tcgggatcat caccgcgcct ctgcagatgc tcttcacgcc cgcggccgtc
      361 atggtcggcc tcgtgtacgg ctacctgccg ttcatgatct tgcccatcta cgcgtccgtc
      421 gaggcgctcg accgccgcta cgtcgaagcc gccaacgacc tcggcgccaa cgactggacg
      481 gcgttctggc gcatcatctt ccccctgacg ctgcccggcg tcatcgccgg ctccatcctc
      541 gtgttcatcc cggccatcgg cgcgtacgtc acgcccgacc tgatgggcgg aacgcgcggc
      601 ctgatgatcg gcaacctgat ccaggggcag ttcggcggcc gcggcaacgt gcccctcggc
      661 agcgccatct ccatggttct gctggccatg gtgttcatca gcgtcctcgt ctacacgcgc
      721 tgggggcagc gggaggagtg atggtccagc cgcggcccgc cccggaccag aagacggtcg
      781 cccgcaaggt gcggcgcgac atggccgtgc gccgcaccgg ccgcttcggg atgtggctca
      841 gcccgttcgt cgtctacctg ttcctctggg tgccgatcat cgtgctcgtc gtgttctcgt
      901 tcaacgacgg caactccgtg agcgtctggc gcggcttcac cacccgctgg tacgagaaca
      961 tcctccacgg caccatctcg gccggcacgg aggcggcgcg cttccagacg gagctcctcc
     1021 tcaaggcgct gcgcaactcg ctcctggtgg cgaccgtggc gacggtgatc agcagcgccc
     1081 tcggcacgat gctctcgctc gcgatcgcgc gcggccgctt ctggggccgc aagacgctcg
     1141 acacgctctt ctacctgcct gtggtcatcc cggacatcac ccagggcatc gcgctcgccg
     1201 tcttcttcaa catcgtcttc gagacggtcc agcggctgtt cggcgtgcgc ctcgtctcgg
     1261 gcttcggcac catcgtgata gcccacgttg cgttcaacat cagctacgtg actatcgtgg
     1321 tgcgcgcgcg gttggccggc atggacccgc gcctggagga ggccggtcgc gacctcgggg
     1381 ccaaccactg gcagacgttc tggcgcatca ccttccccaa cctgctgccc ggcgttatgg
     1441 ccggcgctct gctggcgttc acgctctccc tcgacgactt cgtcatcacc ttcttcaact
     1501 ccggcgtcgg cacgaccacg ctgccgctct tcgtctacgg gatgctcaag cagcgagtgc
     1561 ccccggagat caacgccatc tcgaccctga tgatcctcgc ctcgatcgtc ctcgtgggcg
     1621 gctcgctcct cctccagcag cgcggcaacc gcgacaaggc gtgaccgccc catgaggcgc
     1681 gtctctgccg cggaggccgt caacgtcgtc cgctcgggcg accgcgtgtt catccatagc
     1741 gtcgccgcgg cgccgcaggc gctgatcgcg gccctgacgg cgcgggccgc ggagctgagg
     1801 gacgtcgaga tcgtccagct ccacacggag ggacctgcac cgtacgcggc gcccgagatg
     1861 gcgggctcct tcagggtcaa cgccctcttc gtcggcaaga acgtgcgcga cgctgtcgcc
     1921 cgcggccagg ccgactacgt gcccgtcttc ctcagcgagg tgccggcgct cttccgcaac
     1981 cgggccatgc cgctcgacgt ggccctcatc agcgtctcgc cacctgacaa gcacggcttc
     2041 tgctcgctcg gcgtctcagt cgacgcctcg cgcgccgccg cgcagtcggc gcgcacggtc
     2101 gtcgcccagg tcaacgccgc gatgccgcgc agccacgggg acgggctcat ccacgtcgac
     2161 gacatcgacc tggccgtcga ggaggcggcg cccatcttcg aggtcagcca ggccaagacc
     2221 ggcgagctgg aacgcacgat cggcggcttc gtcgccagcc tggtggagga cggtgcgacg
     2281 ctccagctcg gcatcggcgc catcccagag gccgtcctcg ccgcgctcac cgaccaccac
     2341 gacctggggg tccacactga gatgttctcc gacgggatcg tcgacctcgt cgagcgcggc
     2401 gtggtcaccg gcgcgcttaa gcgcaaccac ccgggcaagg tggtggcgag cttcgtcatg
     2461 ggctcgcgca ggctctacga cttcatcgac gacaacccgt ccgtgctcct gctcgacgtg
     2521 gcctacacga acgacacggc cgtcatccgc cgcaacccga aggtgacggc cgtcaactcc
     2581 gccctcgaga tcgacctgac cggccaggtc tgcgccgact cgctcggcac gttccagtac
     2641 tcgggcgtgg gcggccagat ggacttcatc cgcggcgcc
//
LOCUS       DAOQYE010000190         2670 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_41879_length_2670_cov_3.165583, whole genome shotgun sequence.
ACCESSION   DAOQYE010000190 DAOQYE010000000
VERSION     DAOQYE010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41879_length_2670_cov_3.165583"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="PKN52_04110"
     CDS             <1..412
                     /locus_tag="PKN52_04110"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNQ99158.1"
                     /translation="VAAVVIWQQLGFQVVIFLAGLQGIPRPMQEAASIDGANGWQRFW
                     YVTFPLLNPVLVFSLIISTISTLQMFDQVQNINFTDQGGPLGSTLTIALYMYEQAFQK
                     FRIGYAAAITVLLFAIILVITLVQLKVTQRKVEY"
     gene            412..1269
                     /locus_tag="PKN52_04115"
     CDS             412..1269
                     /locus_tag="PKN52_04115"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNQ99159.1"
                     /translation="MASSAQRIRPTRRRPSFHLGRFLAYILLAVGSVVMAFPFVWMIL
                     SAFKPLKEIFRFNFWPKVWTLANFQDVLLHTQFPRWFLNSLIVAGISTVSVLFFASLV
                     GYTLTRLRFPGRNLIFILILSTLMIPTEMLVIPWYVMSTEYGWVNTFWGIAFPGLIPA
                     FGVFLMRQFFETLPRDLFDAGRVDGVSEFGLFWRVGLPLVGPGLAALGIFNFVGNWNA
                     YLWPLIVAKSPSMRTIPVGVAFFSGEAGTEWNLIMAAAALAIIPVLLVFFIFQRQIIE
                     GVVLTGVKG"
     gene            1266..2294
                     /locus_tag="PKN52_04120"
     CDS             1266..2294
                     /locus_tag="PKN52_04120"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HNQ99160.1"
                     /translation="MSDSPDCSAWGAGRGPDSPRIVVVGNANVDVLMGDIAPWPTPGC
                     EVVIDRYEWRVGGAAGNTGLALAAMGVREGVDSEVIAHVGEDFLGGWLEGAMAGAARL
                     TRVPHPTALTVGLTHPDGQRTFVSYLGHLVALPEERIDAALDSARPGDLLLLCGYFLL
                     PELRAQAPGILRRARGRGMLTMIDTGWPDEGWTPAVRAEVAALLPYLSAFLPNRDELL
                     GVTGGRVTDAADAAAEVLDMGAERVVVKCGPSGALMASAGEQATYPAPSVYVQDTVGA
                     GDTFNAGLLAGLARGMSWADALAPAVSASALAIGSQPRRYPSWPEVAADATASSVTAS
                     CHPLSVTK"
     gene            complement(2210..>2670)
                     /locus_tag="PKN52_04125"
     CDS             complement(2210..>2670)
                     /locus_tag="PKN52_04125"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF013934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HNQ99161.1"
                     /translation="LSALASDATGNQGKRFVWPRTHEAPRAHLTLHAVADVNEPNAWE
                     RHRFGQLEPKSDSPPVDPRDVDLVLVPGLAFDGRGHRLGYGKGYYDALLPLLRPDAVV
                     VGVALDALVVPDVPHDERDFPVRYLVTESGWQDAVTEEAVASAATSGQLG"
BASE COUNT          360 a          974 c          859 g          477 t
ORIGIN      
        1 cgtggcggcc gtcgtcatct ggcaacagct cggtttccag gtcgtcatct tcctggcggg
       61 cctgcagggc atcccgcgcc ccatgcagga ggccgcctcc atcgacggcg ccaacggctg
      121 gcagcggttc tggtacgtga ccttcccgct gctcaacccg gttctcgtgt tctcgctgat
      181 catctccacc atctcgacac tgcagatgtt cgaccaggtg cagaacatca acttcaccga
      241 tcagggcggg ccgctcggta gcacgctgac catcgccctt tacatgtacg agcaggcgtt
      301 ccagaagttc cgcatcggct acgcagccgc tatcacggtc ctcctcttcg ccatcatcct
      361 cgtcatcacc ctcgtccagc tcaaggtcac ccaaaggaag gtcgagtact gatggcctcc
      421 agcgcgcagc gcatccggcc gacccgccgt cggcccagct tccacctcgg ccgcttcctc
      481 gcgtacatcc tcctggccgt cggcagcgtc gtcatggcgt tcccgttcgt atggatgatc
      541 ctctcggcgt tcaagccgtt gaaggagatc ttcaggttca acttctggcc gaaggtctgg
      601 acgctcgcca acttccaaga cgtgctcctg cacacgcagt tcccgcgttg gttcctcaac
      661 agcctgatcg tggccgggat ctcgaccgta tccgtgctct tcttcgcgtc gctcgtcggc
      721 tacacgctca cgcgcctgcg cttcccgggg cgcaacctca tcttcatcct gatcctcagc
      781 acgctgatga tcccgaccga gatgctcgtg atcccctggt acgtcatgtc gaccgagtac
      841 ggctgggtca acacgttctg gggcatcgcg ttccctgggc tcatcccggc gttcggcgtg
      901 ttcctgatgc ggcagttctt cgagacgctg ccgcgcgacc tcttcgacgc cggccgtgtc
      961 gacggcgtga gcgagttcgg cctcttctgg cgggtcgggc tgccgctcgt cgggcccggc
     1021 ctcgccgcgc tcggcatctt caacttcgtc ggcaactgga acgcctacct gtggccgctc
     1081 atcgtggcga agtcgccttc catgcgcacg atccccgtcg gtgtcgcgtt cttctcgggc
     1141 gaggcgggca cggagtggaa cctcatcatg gccgcggcgg ccctagccat catcccggtc
     1201 ctgctcgtgt tcttcatctt ccagcgccag atcatcgaag gcgtggtgct caccggggtg
     1261 aagggttgag cgactccccg gactgtagtg cctggggggc ggggcgaggc cccgactccc
     1321 cacgcatcgt cgtggtcggc aacgccaacg tcgacgtcct gatgggcgac atcgcgccct
     1381 ggccgacgcc cggctgcgag gtcgtgatcg accggtacga atggcgcgtc ggcggagccg
     1441 ccggcaacac ggggttggcc ctggccgcca tgggcgtgcg cgagggcgtc gactccgagg
     1501 tcatcgccca cgtcggggaa gacttcctcg gcggctggct ggagggcgcc atggccgggg
     1561 ccgcgcgact cacgcgcgtg ccccatccca ccgccctgac ggtcggcctc acccacccgg
     1621 acggtcagcg caccttcgtc tcctacctcg gccacctggt cgcgctgccg gaggagcgca
     1681 tcgacgcggc cctcgattcg gcgcggccgg gcgacctgct cctcctctgc ggttacttcc
     1741 ttctgccgga gctgcgcgcc caggcgcccg gcatcctgcg gcgcgcacgc ggacgcggca
     1801 tgctcaccat gatcgacacg ggctggcccg acgagggttg gacccccgcc gtccgcgccg
     1861 aggtggccgc cctgctgccg tacctcagcg ccttcctgcc caaccgcgat gagcttctgg
     1921 gcgttacggg tgggcgcgtt accgacgctg ccgatgccgc cgccgaggtg ctcgacatgg
     1981 gcgcggagcg cgtcgtcgtg aagtgcgggc cgtccggcgc gctcatggcg agcgcgggtg
     2041 agcaagcgac ctacccggcg ccgagcgttt acgtccagga cacggtcggc gcgggcgaca
     2101 ccttcaacgc aggcctcctg gccggcctcg cgcgcggcat gagctgggcg gacgcgctcg
     2161 cccctgcagt gagcgcgtcc gccctcgcca tcggcagcca gccgcggcgc tacccgagct
     2221 ggcccgaagt ggccgcggac gccacggctt cctctgtcac cgcgtcctgc cacccgctct
     2281 cggtgaccaa gtagcgcacc gggaagtcgc gctcgtcatg cggcacgtcg ggcacgacga
     2341 gcgcgtctag cgccacgccg acgaccaccg cgtccggccg caacagcggc agcagggcgt
     2401 cgtagtaccc cttgccgtaa cccaaccgat ggccgcggcc gtcgaaggcg aggccgggca
     2461 ccaggacgag gtccacgtcg cgcgggtcga ccggcggtga atcggacttc ggttccagtt
     2521 gaccgaagcg gtgacgctcc cacgcgttcg gctcgttaac gtcggccacc gcgtgcaggg
     2581 tcaggtgcgc tctcggcgcc tcgtgggttc gtggccagac gaagcgcttg ccttggttgc
     2641 cggtcgcgtc gctcgccagg gcgctcaggt
//
LOCUS       DAOQYE010000191         2670 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_41882_length_2670_cov_2.861950, whole genome shotgun sequence.
ACCESSION   DAOQYE010000191 DAOQYE010000000
VERSION     DAOQYE010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41882_length_2670_cov_2.861950"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            201..1649
                     /gene="sufB"
                     /locus_tag="PKN52_04130"
     CDS             201..1649
                     /gene="sufB"
                     /locus_tag="PKN52_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HNQ99162.1"
                     /translation="MSDLHTDTTQAVHPDAAKLEGMEHTKQYEFKLAEDYFFKSDRGL
                     TKRVVEQISYMKNEPEWMLKFRLRAFEIAEKKGRPKWGPDLSDLNFDDIFFYVRPNEK
                     QGTAGSWDEIPEEVRKTYQRLGVPEAEQRALAGVGAQYESEMVYHKLKAEWEKLGVIF
                     LDTDTGLKEHPEIFKEHFATVIPPEDNYFAAVNSAVWSGGSFVYVPEGVHVEVPLQAY
                     FLLNAENIGQFERTLIIGAPGSKFHYIEGCTAPAYTSNSFHSGVIEIICQERSSVRYS
                     TIQNWSHNVYNLVTQRAMVHERASMGWLDGNLGSKVTMKYPSTYLVGEGAHGEVLSIA
                     FATDGQHQDAGAKVIHVAPNTTSSVVSKSISKGTGRSSYRGLVQVHEGATNARSNVEC
                     DALLLDENARTDTFPYIEINEKTAHVGHEATVSKLDDEQIFYLMTRGLAEDAAMALIV
                     RGFLDPVAKELPLEYAVELNRLIELEMEGSVG"
     gene            1651..>2670
                     /locus_tag="PKN52_04135"
     CDS             1651..>2670
                     /locus_tag="PKN52_04135"
                     /inference="COORDINATES: protein motif:HMM:NF013614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HNQ99163.1"
                     /translation="MAALTDNPVVSTDAVESIVSAYREPAWLADERRAALRAYTDQPF
                     PTEKTEEWRYTQLRRFSFAGLELAKAPKDESVPERIRMRIADSDAEGVIVHKGNKVVL
                     HQAAIKEAGVVFTDLRTALRDHEALVREHLYDVVNARQTKFTALNSALWENGTFVYVP
                     RDVEVELPLGAFTTADAGGIGLGRTLIVVDVNARLTFIDEYTSEPFDERLFFDAATEI
                     VLKDGAKLRYVSLQNWSRNVAHINKLRAHLGRDARLESVTVSLGADAARAEVESRLEG
                     PGSESEMLGLYFADEGQHFNQFTLQHHATPRGFSDVLFKGAVRDASQAVYSGMIIVDP
                     HAQKTD"
BASE COUNT          510 a          944 c          845 g          371 t
ORIGIN      
        1 gcgcgctcgt catcacccac taccagcgcc tcctcaacca catcgtcccc gacaaggtgc
       61 acgtgatggt cggcggccgg gtcgtgcgcg agggcccgaa ggagctcgcc atggagctcg
      121 accagaaggg ctacgagtgg atccgcgagg ctgtcggcgc ctgacgggcg cccgacgcga
      181 agggctgaag ggacaccaga gtgtctgacc tgcataccga taccacccaa gccgtccacc
      241 ccgacgccgc caagctcgag gggatggagc acaccaagca gtacgagttc aagctcgcgg
      301 aagactactt cttcaagtcc gaccgcggcc tgacgaagcg cgtcgtcgaa cagatcagct
      361 acatgaagaa cgagccggag tggatgctca agttccgcct ccgcgcgttc gagatcgccg
      421 agaagaaggg ccgcccgaag tggggccccg acctctccga cctgaacttc gacgacatct
      481 tcttctacgt gcggccgaac gagaagcagg gcacggccgg ctcgtgggac gagatccccg
      541 aggaagtgcg caagacctac cagcgcctgg gcgtgccgga ggccgagcag cgcgcgctgg
      601 ccggtgtcgg cgcgcagtac gagtccgaga tggtctacca caagctcaag gcggagtggg
      661 agaagctcgg cgtcatcttc cttgacacgg acacgggcct gaaggagcac cccgagatct
      721 tcaaggagca cttcgccacc gtcatcccac cggaggacaa ctacttcgcg gccgtcaact
      781 cggccgtgtg gtcgggcggc tcgttcgtct acgtgcccga aggcgtgcac gtcgaggtgc
      841 cgctccaggc gtacttcctg ctgaacgcgg agaacatcgg ccagttcgag cgcacgctca
      901 tcatcggcgc gccgggctcc aagttccact acatcgaggg ctgcacggcc cccgcgtaca
      961 cgagcaactc gttccactcc ggcgtcatcg agatcatctg ccaggaacgc agcagcgtgc
     1021 gctactcgac catccagaac tggtcgcaca acgtctacaa cctcgtcacg cagcgcgcca
     1081 tggtccatga gcgcgccagc atggggtggc tcgacggcaa cctcggctcc aaggtcacga
     1141 tgaagtaccc gagcacctac ctcgtcggcg agggcgcgca cggcgaggtc ctgtccatcg
     1201 cgttcgccac cgacggtcag caccaggacg ccggcgcgaa ggtcatccac gtcgccccga
     1261 acacgacgag cagcgtggtc agcaagtcca tctcgaaggg cacggggcgc tccagctacc
     1321 gcggcctcgt ccaggtgcac gagggcgcga ccaacgcgcg ctcgaacgtg gagtgcgacg
     1381 ccctcctcct cgacgagaac gcccgcaccg acaccttccc gtacatcgag atcaacgaga
     1441 agacggcgca cgtcggccac gaggccaccg tgagcaagct cgacgacgag cagatcttct
     1501 acctcatgac gcgcggcctc gccgaggacg ccgccatggc gctgatcgtg cgcggcttcc
     1561 tcgacccggt ggccaaggag ctgcccctcg agtacgcggt cgagctcaac cggctcatcg
     1621 aactcgagat ggaagggagc gtcggctgac gtggcggccc tgaccgacaa cccggtcgtc
     1681 agcacggacg ccgtcgagag catcgtctcc gcctatcggg agcccgcgtg gctggccgat
     1741 gagcgccgcg ccgcgctgcg cgcctacacc gaccagccgt tcccgaccga gaagacggag
     1801 gagtggcgtt acacgcagct caggcgcttc tcgttcgccg ggctcgagct cgccaaggcg
     1861 cccaaggacg agagcgtgcc ggagcgcatc cgcatgcgca tcgccgactc cgacgccgag
     1921 ggcgtgatcg tccacaaagg caacaaggtc gtcctgcacc aggccgccat caaggaggcc
     1981 ggcgtcgtct tcaccgacct gcgcacggcg ctccgcgacc acgaggcctt ggtgcgcgag
     2041 catctctacg acgtagtcaa cgcgcgccag acgaagttca cggccctcaa ctccgccttg
     2101 tgggagaacg gcaccttcgt gtacgtgcca agggacgtcg aggtcgagct gccgctcggc
     2161 gccttcacga ccgccgacgc cggcggcatc gggctggggc gcacgctcat cgtcgtcgac
     2221 gtcaacgcgc gcctcacctt catcgacgag tacacgagcg agccgttcga cgagcgcctc
     2281 ttcttcgacg cggccaccga gatcgtgctg aaggacggcg ccaagctccg gtacgtcagc
     2341 ctccagaact ggagccgcaa cgtcgcccac atcaacaagc tgcgggcgca cctcggcagg
     2401 gacgcgcgcc tcgagtcggt caccgtcagc ctcggagcag acgccgcgcg cgccgaggtc
     2461 gagagccggc tcgagggtcc cggctccgag agcgagatgc tcggcctcta cttcgccgac
     2521 gagggccagc acttcaacca gttcacgctc cagcatcacg cgaccccgcg cggcttctcg
     2581 gacgtactgt tcaagggcgc cgtgcgcgac gcgagccagg ccgtctactc cggcatgatc
     2641 atcgtcgacc cgcacgcgca gaagacggac
//
LOCUS       DAOQYE010000192         2670 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_41890_length_2670_cov_2.208413, whole genome shotgun sequence.
ACCESSION   DAOQYE010000192 DAOQYE010000000
VERSION     DAOQYE010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41890_length_2670_cov_2.208413"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..939)
                     /locus_tag="PKN52_04140"
     CDS             complement(<1..939)
                     /locus_tag="PKN52_04140"
                     /inference="COORDINATES: protein motif:HMM:NF020390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HNQ99164.1"
                     /translation="MHLEPSRLVDADLHLVNRLTWGARPRDLERVRELGEDGYLEWQL
                     DYAAIDDPLVDRFLAEHPVLLEDVRGIRRALDEDYGAVYQQVIWGRLYRAAYSERQLY
                     EKMVEFWTDHFNVPLPDMVPEKVVDDREVARTHALGRFRDLLMASARSPGMLIYLDNT
                     FSNRDHPNENYSREVMELHTLGVDGGYTQQDVVEVARCFTGWTLDQEWRGSFVLDRNR
                     HDEGEKVVLGHRIAAGRGIEDGLQVIDILATHPSTARFISKKLCKRFVSDDPPADLVD
                     AVATTFLATDGDIRATLRALFTSEGFRAARGTKFRRP"
     gene            990..1715
                     /locus_tag="PKN52_04145"
     CDS             990..1715
                     /locus_tag="PKN52_04145"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNQ99165.1"
                     /translation="MSPSERVDAMHRIYLVEDEPKLAAMLAAHLGRYGYEVVPARRFD
                     DLKNEFLASGAGLVLLDVNLPWYDGFYWCRQIRTASNAPVIFISARSGEMDQVLAIDN
                     GGDDYIVKPFNLEVVTAKVRGALRRAYGEYANAGEQTLTSAAGLAFDPLRLVVAFDGR
                     QVELSRNEGQLLEVLLRANGKVVGREALLEALWDDAAFVDDNTLTVNVNRLRRKLTEL
                     GLGDAVRTVRGAGYALQPSDGDA"
     gene            1761..>2670
                     /locus_tag="PKN52_04150"
     CDS             1761..>2670
                     /locus_tag="PKN52_04150"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNQ99166.1"
                     /translation="MRPLDYLLDHLSLIIGFAVALLFLLLVVHLAVARLHAVDAGYIL
                     LLGGVSGAICLLVDYLRQRRFRRAVARLLGADREADPTTGFPLLQLPPAKSREQRALA
                     ELLTGSQRSALEALQRHRRGAEEHRAFVDLWVHHMKTPLAVLEVTVQQQDGQGQDADS
                     EWRSVREEVDELGRGLELMLAASRLERFELDLRLVTVDLVGVVRASVNELRRAWIRSG
                     VFPSVDAPSGPVEVETDPKWLQVVLRQLLTNAMKYSEAGQKVTVGIRPLGGGGATVSV
                     SDDGIGIPPEDVPRVFDRFFTGANGRR"
BASE COUNT          415 a          865 c          950 g          440 t
ORIGIN      
        1 cgggcgccgg aacttggtgc cccgggcggc gcggaagcct tccgaggtga agagcgcgcg
       61 cagcgtggcg cggatgtcgc cgtccgtcgc caggaacgtg gtggcgacgg cgtcgacgag
      121 atccgccggc gggtcgtccg agacgaagcg cttgcatagc ttcttgctga tgaaccgcgc
      181 cgtggacgga tgggtggcga ggatgtcgat gacctgcaag ccatcctcga tgccgcgccc
      241 ggcggcgatc ctgtggccga gcacgacctt ctcgccctcg tcatggcggt tgcggtcgag
      301 cacgaagctg ccgcgccact cctggtccaa cgtccaaccg gtgaagcagc gcgccacctc
      361 gaccacgtcc tgctgcgtgt agccgccgtc gacgccgagc gtgtgcagct ccatcacctc
      421 gcgcgagtag ttctcgttgg ggtgatcgcg gttgctgaag gtgttgtcga ggtagatgag
      481 catgccaggg ctgcgcgcgg aggccatgag caggtcgcgg aagcgcccga gggcatgcgt
      541 ccgcgccacc tcccggtcgt cgaccacctt ctccgggacc atgtctggca ggggcacgtt
      601 gaagtgatcg gtccagaact cgaccatctt ctcgtagagc tggcgctcgc tgtaggcggc
      661 ccggtagagg cgcccccaga tgacctgctg gtagacagcg ccgtaatcct cgtcaagggc
      721 gcggcgtatc ccgcgaacgt cctcgagcag cacagggtgt tccgccagga agcggtcgac
      781 gagcgggtcg tctatggccg cgtagtcgag ttgccactcg aggtagccgt cctcgccgag
      841 ttcgcgcacg cgctcgaggt cgcgcggcct ggcgccccac gtcaggcggt tcaccaagtg
      901 caagtcggcg tcgaccaagc gcgagggttc taggtgcatg gtcaataatc gagcctatgg
      961 tgaccgggcg catgagaggt tgttaacgta tgagcccaag cgagcgtgtc gacgccatgc
     1021 accggatcta cctggtcgaa gacgaaccca agctggccgc catgttggcc gcgcacctgg
     1081 ggcgctacgg gtacgaggta gtgcccgcgc ggcgcttcga cgacctaaag aacgagttcc
     1141 tggcctctgg cgccgggctc gtgctcctcg acgtcaacct gccgtggtac gacggcttct
     1201 actggtgcag gcagatccgg accgcctcca acgcgcccgt tatcttcatc tcggcgcgct
     1261 caggcgagat ggaccaggtg ctggcgatcg ataacggcgg cgacgattac atcgtcaaac
     1321 cgttcaacct cgaggtcgtt acggccaagg tgcgcggcgc gctgcggcgc gcgtacggcg
     1381 agtacgccaa cgcgggtgag cagaccctga cgtccgccgc cgggctcgcc ttcgacccct
     1441 tgcggctggt ggttgccttc gacggtcgcc aagtggagct gagccgcaac gagggtcaac
     1501 tcctcgaggt cctcctgagg gccaacggca aggtcgtcgg gcgggaggcg ctcctcgagg
     1561 ccctgtggga cgacgcggcg ttcgtcgacg acaacacgct caccgtgaac gttaaccgcc
     1621 tgcgccggaa gctgacggag ctggggctcg gagacgcggt ccggaccgtg cggggcgcgg
     1681 gttacgcgct ccaaccgtct gatggtgacg cgtgaggtgc gagtgatcct cctgcgtggg
     1741 aaaaccgagt tcggccgacc atgaggccgc tggactacct gctcgaccac ctgtcgctca
     1801 tcatcggctt cgccgtcgcg ctcctcttcc tcctgctggt cgtgcacctg gccgtcgcgc
     1861 ggttgcacgc ggtcgacgcc gggtacatcc tgctgttggg tggcgtgagc ggggcgatct
     1921 gcctgctcgt cgactacctg aggcagcgtc ggttccgccg ggcggtggcg cggttgctcg
     1981 gggccgaccg ggaagcggac cccaccaccg gcttcccgct ccttcaactc ccgcccgcga
     2041 agtcgcgcga gcagcgcgcg ctcgccgagc tcctgaccgg ctcgcagcgt tcggcgctgg
     2101 aggcgttgca gcggcaccga cgcggcgccg aggagcatag ggcgttcgta gacctctggg
     2161 tgcatcacat gaagacgccc cttgccgtgc tggaggtgac ggtccagcaa caggacggcc
     2221 agggtcagga cgccgacagc gagtggcgga gcgtccggga ggaggtcgac gagctcgggc
     2281 gcggcttgga gctgatgctc gccgcctccc gactcgagcg gttcgagctc gacttgaggc
     2341 tggtcaccgt ggacttggtc ggggtagtcc gggcgagcgt gaacgagctc aggcgggcct
     2401 ggatccgcag cggcgtcttc ccgagcgtcg atgcaccaag cgggccagtc gaggtcgaga
     2461 ccgacccgaa gtggctccag gtcgtactgc gccagctcct caccaacgcg atgaagtaca
     2521 gcgaggccgg gcagaaggtc acggtcggca tccgaccgct tggcggcggc ggggcgacgg
     2581 tcagtgtgtc ggacgacggc atcggtatcc caccggagga cgtgccgcgc gtgttcgacc
     2641 ggttcttcac gggcgccaac ggcaggcgca
//
LOCUS       DAOQYE010000193         2666 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_41985_length_2666_cov_2.193029, whole genome shotgun sequence.
ACCESSION   DAOQYE010000193 DAOQYE010000000
VERSION     DAOQYE010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2666
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41985_length_2666_cov_2.193029"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="PKN52_04155"
     CDS             <1..508
                     /locus_tag="PKN52_04155"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNQ99167.1"
                     /translation="ERTPEGGLISFDRLLAYPIDEVWSALTEPERLADWWAPFAAAIT
                     IDLREGGSLSFDWLEHDIPRFEFTILRFEPPTLLEHTHTGPGSWMRWELEPTTEGTRL
                     RATYFVPDPDLAIERGDVVGAHYGLDRLEAVLSGQPIPVDMEVFAALAADYAEHELIA
                     SAGGERSG"
     gene            727..1080
                     /locus_tag="PKN52_04160"
     CDS             727..1080
                     /locus_tag="PKN52_04160"
                     /inference="COORDINATES: protein motif:HMM:NF017757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNQ99168.1"
                     /translation="MVWAGRPWEDVASGVLGLDDETYYLVAAAIARLESEGPALGRPT
                     ADRIEGSRHHNMKELRPGSSGRSEIRILFAFDPGRIAVLSVAGDKAEMWKQWYERNIP
                     VADDRFDEWLNSEVL"
     gene            1082..1408
                     /locus_tag="PKN52_04165"
     CDS             1082..1408
                     /locus_tag="PKN52_04165"
                     /inference="COORDINATES: protein motif:HMM:NF025120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HNQ99169.1"
                     /translation="MNEAKTISWSEVRAERPVDAAVVAAHEARMEAEERAYELRQIRE
                     EQGVTQKELAERMALTQPTISALEAGDLYRSGLSTLKAYVEALGGTVELTARFGSRRL
                     VLSPRK"
     gene            complement(1503..2459)
                     /locus_tag="PKN52_04170"
     CDS             complement(1503..2459)
                     /locus_tag="PKN52_04170"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNQ99170.1"
                     /translation="MNSSHSLHVIVGAGPVGTSLARRLAQEGERVRVLTRSGRDFGVP
                     GVESLAVDATDVGKLTAAARGAQVLYNCANPGTYSAWEKEWPPLANAILAAAESTGAV
                     LVTASNLYGYGPVGGPMTRATPLRPSDHKGALRARLWREALAAHEAGRVRVAEARASD
                     YIGPALSIGNGVLARYAHATLGGKPAMVFADPDQPHTWTAIKDVAATLAVLGQDERAW
                     GSAWIVPSNPALSVRRTLLDLHGECGLPEPRLRQVPRWTMRAGGAFVPLLREVTGVLY
                     QFDAPFVADGSETTEQLGVAPTPWERVVRDTAAAWLARARVR"
BASE COUNT          420 a          894 c          905 g          447 t
ORIGIN      
        1 cgaacgcacg ccggagggcg gcttgatcag ctttgatcgt ctgctcgcct accccatcga
       61 tgaggtctgg tccgcgctca cggagcccga gcgcctcgct gactggtggg cgcctttcgc
      121 cgctgccatc acgatcgacc tacgggaggg cggctccctc tccttcgatt ggctagaaca
      181 cgacatcccg aggttcgagt tcacgatcct gcgcttcgag ccgccgacgc tgctcgagca
      241 cacgcacact ggccccggct cctggatgcg gtgggagctg gagcccacca ccgagggtac
      301 aagactgcga gccacctatt tcgtaccgga cccggacctg gccatcgagc gtggcgatgt
      361 cgtgggcgcg cattatggcc tcgaccgtct cgaggccgtg ctctcgggcc agccgatccc
      421 ggtcgacatg gaagtcttcg ctgcgttggc agccgactac gcggagcacg agttgattgc
      481 gtcggcagga ggggaaaggt ccggttagcc cgccgggacg tggggggctg tcgacgcgga
      541 ggcgagcagc cttcatgccg cgcgacctga tgccggacgc tctccgcgtc atcagcgact
      601 ggctgccgct cagttacgcc atcgacgcgg ttactgccgt cagggcagtc cgggcggctg
      661 ggggctgtgg cggccgctgc ccgacataga gttgcatcta tatcgagtga gatctatact
      721 aaacgcgtgg tctgggcggg gcggccctgg gaggacgtcg cgtcaggggt cttggggttg
      781 gatgacgaga cctattatct ggttgccgcc gcgatcgcaa gattggagtc cgaggggcca
      841 gcgctcgggc gcccgaccgc agatcggatc gagggctccc gtcaccacaa catgaaggag
      901 cttaggcctg gttcgtctgg acgcagcgag atccggatcc tattcgcctt cgacccagga
      961 cgtatagccg ttctctcggt agcaggagac aaggccgaga tgtggaagca gtggtacgag
     1021 cggaacatcc cggtagctga tgacaggttc gatgagtggc tcaacagcga ggtgttgtga
     1081 gatgaacgaa gccaagacga tttcctggag cgaagtgcgc gccgaacgtc cggtcgacgc
     1141 tgccgtggta gccgcgcacg aggcacgtat ggaggcggag gagcgtgcct acgagctccg
     1201 ccagatccgc gaggaacagg gggtaacgca gaaggagctt gctgaacgca tggctctcac
     1261 ccaacctact atctccgctt tggaagcggg cgatctctat cgctccgggc tgtccaccct
     1321 caaggcatat gtcgaagcac taggcggcac cgttgagctc acggccaggt tcggaagtcg
     1381 cagactggtc ctctcgcccc gcaagtagac acggcctcgg cgggtccgtc tcagcgtcag
     1441 tcgctgtgct cctttgacgt ccgactcaag tcaccacgga ggttagcgcg gtccggaatg
     1501 acctaccgca cccgcgccct ggccagccac gcggctgcgg tgtcgcggac gacccgctcc
     1561 cacggcgtcg gggcgacccc gagctgctcg gtcgtctccg atccatcggc gacgaacggc
     1621 gcgtcgaact ggtacaggac gcccgtgacc tcccggagca gcgggacgaa cgcgcccccg
     1681 gcacgcatcg tccaccgggg cacctgccgt agccggggtt cggggagccc gcattcgccg
     1741 tgcaggtcga gcaaggtccg gcgaacgcta agcgccgggt tggacggcac gatccacgcc
     1801 gagccccacg cacgctcgtc ctgaccgagc accgcgagtg tggcggcgac gtccttgatg
     1861 gccgtccagg tgtggggttg gtcgggatcg gcgaatacca tcgccggctt cccccctagc
     1921 gtcgcgtgtg cgtacctcgc cagcacgccg ttgccgatcg acaacgccgg gccgatgtag
     1981 tcggacgccc ttgcctcggc gacgcgcacc cgcccagcct cgtgggccgc cagcgcctcc
     2041 cgccacagtc gcgctcgcag cgcgcccttg tggtcgctgg ggcgcaacgg cgtcgcgcgc
     2101 gtcatgggtc cgccgacagg accgtagccg tagaggttgc tcgccgtcac cagcaccgcg
     2161 ccggtgcttt ccgccgctgc caggatcgcg ttcgccagcg gcggccactc cttctcccag
     2221 gccgagtacg ttcccgggtt ggcgcagttg tagagcacct gtgctccgcg cgcggcggcc
     2281 gtgagcttgc ctacgtcggt cgcgtctaca gcgagcgact cgacccccgg cacgccgaag
     2341 tcgcggcccg agcgggtcag cacccgcacg cgctcgccct cctgcgcgag tcgtcgcgcc
     2401 agcgaggttc cgaccggccc ggcgccgacg atcacgtgaa gggagtggct tgagttcata
     2461 tggctattgt ctcgcgcttc cggctcggga cagcgagcgt acaggagtcg cgtggtcagc
     2521 gggatcgtcc gcgcggatcc gctggcgcgc cgtctcgccg cgccgcccga cgggcaagtg
     2581 ctcacctgcc gcggccagcc cgatcacggg catgttgttg tacacggtct cataggcgcc
     2641 cgcggcgacc ttgaaggtct cgtgga
//
LOCUS       DAOQYE010000194         2660 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_42103_length_2660_cov_3.234933, whole genome shotgun sequence.
ACCESSION   DAOQYE010000194 DAOQYE010000000
VERSION     DAOQYE010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2660
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42103_length_2660_cov_3.234933"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /locus_tag="PKN52_04175"
     CDS             <1..559
                     /locus_tag="PKN52_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99171.1"
                     /translation="FEHEAARTYDDDGLLLHSEPQIGGSTIVLAERKPNWPYLPSLLH
                     VWVDDADITLERAGRWEALPFTATYSSLPPGFRLDGRYLSLGGGGDIAFGGRTSVTSW
                     REFLFFPDGVVVRGAGVCGTSEFGDTRVVVTDLPPDRRGRYAVDGVIPTTRFDDGSTY
                     DYVLVTDSTDSKDVIWLDGVAYTRR"
     gene            616..879
                     /locus_tag="PKN52_04180"
     CDS             616..879
                     /locus_tag="PKN52_04180"
                     /inference="COORDINATES: protein motif:HMM:TIGR01552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HNQ99172.1"
                     /translation="MAKVGIRELRQNASTVVAKATKGETVTITDRGRPVAEMRPVSTS
                     ILDRLVESGHARPPRRDIRKLPAPEPGPNLSAALAAMRDAERY"
     gene            882..1274
                     /locus_tag="PKN52_04185"
     CDS             882..1274
                     /locus_tag="PKN52_04185"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNQ99173.1"
                     /translation="MVYYIDTSALVKLVVAEHETAALLAWIDGLGPELVACDLARTEL
                     LRAVRRTALDRLVQARTVLDSIAIVSVRTTTFETAGRLEPAELRTLDALHVAAALEFG
                     DELAGIVTYDDRFAEAARRQGLTVIAPA"
     gene            1415..1491
                     /locus_tag="PKN52_04190"
     tRNA            1415..1491
                     /locus_tag="PKN52_04190"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1449..1451,aa:Arg,seq:acg)
     gene            complement(1598..>2660)
                     /locus_tag="PKN52_04195"
     CDS             complement(1598..>2660)
                     /locus_tag="PKN52_04195"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HNQ99174.1"
                     /translation="QARLTELLQTRNQGKLRPRSKITLADLVSEWLKHKEGSGVSART
                     LASYRNALNTYVLPVLGHRRVADLTLREVERLYADMRAGALPTEAKDAGWRGDPLGAR
                     SVQITHTALHQVLKQAVRHGLTALNPIDEASVPSAKSVEKRTLTVAEREAFLTAAIEQ
                     GAFYRVLYQALMDTGMRPGEACALTWTDVDFVGEQLTITKAITRGKDGQRISAAPKTA
                     KSRRTIPMFGLRDVLLEHRRWQAEVGLGGTGHVFTNQEGGILAPWAMNKRELDRVTTA
                     AGITGFTLYGFRHTFATLHLQSGTPLKIVSEWLGHATIQQTANTYQHLITEVSMNYAE
                     RHVQWLRQEAKAARQAVVN"
BASE COUNT          420 a          829 c          907 g          504 t
ORIGIN      
        1 cttcgagcat gaggcagctc gcacatacga cgatgacggc ctgctgctgc actccgagcc
       61 ccagatcggc ggctcgacca tcgtcctggc ggagcgcaag ccgaactggc cgtacctccc
      121 ttccctgcta catgtctggg tcgacgacgc cgacatcacc ttagagcgcg ccggccgctg
      181 ggaggcactg ccattcaccg ccacgtactc gagtctgccc ccaggcttcc ggctggacgg
      241 tcgctacttg tcgctcggcg gcggcggcga catcgcgttc ggcgggagga catcagtgac
      301 cagctggaga gagttcctgt tcttcccgga cggcgtggtc gtgcgcggcg ccggtgtgtg
      361 tggcacgagc gagttcggcg acaccagggt ggtggtgacc gacctgcctc ccgaccggcg
      421 cggacggtac gccgtagacg gcgtcatacc gaccacgcgg ttcgatgacg gctcgaccta
      481 cgactacgtg cttgtcaccg attcgaccga ctccaaggac gtcatctggc tcgacggggt
      541 cgcctacacg cgaagatgag gggctcggtg cccaacacac tggtcgccca agcctgatgt
      601 gctacattgt agcacatggc gaaggtaggg ataagggaac tcaggcagaa cgcctcgacg
      661 gtcgtcgcta aggctaccaa gggggaaacc gtgaccatca cggaccgcgg ccggccggtg
      721 gcggagatga ggccggtctc cacctccata ctggaccggt tggtcgaatc cgggcacgct
      781 cgcccgccac ggcgcgatat caggaagctg ccagcgcccg agccggggcc gaacctctcc
      841 gctgcactgg ccgccatgcg ggacgccgag cgctactgaa gttggtctat tacatcgaca
      901 cttctgcgtt agtgaagctg gtcgtcgccg aacacgagac ggcggccttg ttggcctgga
      961 tcgacggcct aggaccagaa ctggtcgcct gcgatctcgc gcgaaccgag ctcctacgag
     1021 cagtgcggag aacggccctc gacaggctgg tgcaagcccg cacggtattg gattcgatag
     1081 ccatcgtgtc cgtgcgtacg acgacgttcg agacggccgg aaggctggaa ccggcggagt
     1141 tgcgaacgct tgacgcgctt cacgtggccg cggcgctcga gttcggcgac gagcttgccg
     1201 gtatcgtcac gtacgacgac cgcttcgccg aggcggcgcg tcgacagggc ctgacggtga
     1261 tcgcgcccgc ttgagcgggt ctccgccgcc tcacaccggt cggtgagacc gcgcaggtag
     1321 gtgaaaggtg cgcgcccgaa accttgcccc cttgcaccga gcgcgctgcc ccgtgatatc
     1381 ctttcgtgcc cgggccgcct aggctcgcaa gcacgcgccc gtagctcagt tggatagagc
     1441 gtctgactac ggatcagaag gtcaggagtt cgaatcttct cgggcgcacc agcaggacac
     1501 gcgttgcaca cgcgattgat gggaaggacg cctccgtgcc agcgggggcg tccttcgttg
     1561 acgttgggtc accgttggcc caactgtttt ctgttcctca gttgacgacc gcctgcctgg
     1621 cagccttggc ctcctgtctg agccattgca catggcgctc ggcgtagttc atcgagacct
     1681 cagtgatgag gtgctggtag gtgttggcgg tctgttggat ggtcgcgtgg ccgagccact
     1741 cgctcacgat cttgagtggc gttccgctct gaaggtggag cgtcgcgaag gtgtgccgga
     1801 agccgtagag ggtgaaccct gttatgcccg cagccgtagt tacgcggtct agctcgcgct
     1861 tgttcatcgc ccagggtgcg aggatgccgc cctcctggtt ggtgaagacg tggccggtgc
     1921 cgcccagtcc tacttcggct tgccacctgc ggtgctcgag gagcacgtcg cgtagaccga
     1981 acatgggaat ggttcgacgg ctctttgccg tcttgggcgc agccgagatg cgctggccgt
     2041 ccttgccgcg ggtgattgcc ttcgtgatgg tcagttgctc gcccacgaag tccacgtcgg
     2101 tccatgtcag ggcgcaggcc tcgccagggc gcatgccggt gtccatgagg gcctgataga
     2161 gcactcggta gaaggcgccc tgttcgatgg cggcggtcag gaacgcttcg cgctccgcca
     2221 cggtgagggt gcgtttctct acgctcttgg cgcttggcac gctagcttcg tctatcgggt
     2281 tgagcgctgt aagcccgtgc ctaacggcct gcttgagcac ttggtggagc gcggtgtgcg
     2341 tgatctgcac actccgagcg ccgagtggat ctccgcgcca gcctgcgtcc ttggcctcgg
     2401 tagggagggc gccggcgcgc atgtcggcgt agaggcgctc gacttcgcgc agggtgaggt
     2461 cggccacgcg gcggtgtcct agtaccggca ggacataggt gttgagcgcg ttccggtagg
     2521 aggctagggt gcgggcagag acgccactcc cctccttgtg cttcagccac tcgctaacta
     2581 ggtcggcgag ggtgatcttg ctacggggtc ggagcttgcc ctggttacgg gtctgcaaga
     2641 gctccgtgag gcgcgcctgg
//
LOCUS       DAOQYE010000195         2658 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_42157_length_2658_cov_3.892432, whole genome shotgun sequence.
ACCESSION   DAOQYE010000195 DAOQYE010000000
VERSION     DAOQYE010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2658
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42157_length_2658_cov_3.892432"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /locus_tag="PKN52_04200"
     CDS             <1..368
                     /locus_tag="PKN52_04200"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNQ99175.1"
                     /translation="VQARADAGYFSTPFPIDLYESGDDVILEMAVPGLKPDQLDISLE
                     GRQLSIRGTVPDVEEGGEGNGRRYWSRGIMRGEFQRTVTIPAGVDPEQISARVDQGLL
                     ILTMPKAVEAKARKITVQG"
     gene            complement(437..1228)
                     /locus_tag="PKN52_04205"
     CDS             complement(437..1228)
                     /locus_tag="PKN52_04205"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNQ99176.1"
                     /translation="MNLGLTGKRAIVLAGTSGLGRGTATALASEGARVVVCGRSPERT
                     RAVAGEIAAATGNGTVAGLPCDVSNGADLERFFAEATAELGGIDILVANAGGPPAGGF
                     DALTDATWAQAFELTLMSVVRSIRLALPSMRAAGRGSIVVIGSSSVRAPIKNLTLSNA
                     YRPAVQALCKDLATNLAPEGVRVNVVSPGRIDTPRIRELDGKASEREGISLEQARARS
                     VASIPLGRIGTVEEFGRVAAFLASDASSYVTGVSLLVDGGLVTSL"
     gene            1505..1921
                     /locus_tag="PKN52_04210"
     CDS             1505..1921
                     /locus_tag="PKN52_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99177.1"
                     /translation="MALTEVIVLIPEERVGDFYRVVGAWMGGANLVDEANAGRSRRRS
                     ASSSSTPLVLSDSSRYHPLHVHLAGVSKDIKTYEMSFDEITTVMGARLPKSAEDHRAW
                     WANTESHSQALAWISAGWKVDGVDLNNRLIHFTRPK"
     gene            complement(2109..2636)
                     /gene="msrA"
                     /locus_tag="PKN52_04215"
     CDS             complement(2109..2636)
                     /gene="msrA"
                     /locus_tag="PKN52_04215"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HNQ99178.1"
                     /translation="MLAGGCFWCMEAVFVGLNGIESVQSGYSGGTVADPSYEQVCTGA
                     TGHAEAVRLEFDPSVVSYADILRVFFTMHDPTTKDRQGNDIGTQYRSVVFYLDDQQRE
                     TAEAVMREIGESGIYGAPLVTELEPLKNFYPAEEYHDDYFARNPRQPYCAAVIAPKVA
                     KFRKLYLDRLRPGYA"
BASE COUNT          452 a          901 c          876 g          429 t
ORIGIN      
        1 aggtgcaggc ccgcgccgac gccggctact tctcgacgcc gttcccgatc gacctctacg
       61 agagcggcga cgacgtcatc ctcgagatgg ccgtgccggg gctcaagccg gaccaactgg
      121 acatcagcct ggaagggcgg cagctgagca tccggggtac cgtccccgac gttgaggagg
      181 gcggcgaggg gaacggtcgc cgctactggt cccgcggcat catgcgcggc gagttccaac
      241 gcaccgtcac catcccggcc ggcgtcgacc cggagcagat ctccgcgcgc gtcgaccagg
      301 gcctcctgat cctcacgatg ccgaaggccg tggaggcgaa ggcccggaag atcaccgtcc
      361 agggttgagc ttcagggttg ggaatgccca accggtccca acttgtgagc caccgcccgc
      421 gcggtggctc actggctcat aaggatgtga ccagaccgcc gtcgaccagc aggctgacgc
      481 cggtcacgta agacgaggcg tcggacgcga ggaacgcggc gacgcgcccg aactcctcca
      541 cggtgccgat gcggccaagg gggatgctgg ctacggaacg ggcgcgcgcc tgctcgaggc
      601 ttatgccttc ccgctccgac gccttgccgt ccaactcgcg gatgcgcggc gtgtcgatgc
      661 gaccggggga gacgacgttc accctcaccc cttccggcgc caggttcgtg gccaggtcct
      721 tgcagagggc ttgcaccgcc ggccggtagg cgttggacag ggtcaggttc ttgatgggcg
      781 ctcgcacgct cgacgagccg atcacgacga tcgagccgcg gcccgccgcc cgcatgctgg
      841 gcagtgctag tcgtatggag cgcacaacgc tcatcagcgt cagttcgaac gcttgcgccc
      901 aggtggcgtc ggtgagcgcg tcgaacccgc cggcaggcgg tccgcccgcg ttggcaacga
      961 ggatatcgat tccgccaagt tcggccgtgg cttcggcgaa gaagcgttcg agatcggcgc
     1021 cgttggacac gtcgcacggc aaacccgcca cggtgccgtt gccggtcgcc gccgctatct
     1081 cacccgccac agcgcgtgtg cgttccggcg acctgccgca gacgaccacg cgcgcgccct
     1141 cggaggccag ggccgtggcg gtgccgcggc cgaggccgct cgtgccggcg agtactatcg
     1201 cccgcttacc ggtaagtcca aggttcatcc tagcctccaa aaccgcatct gtgacatcct
     1261 catggcgcta gcgtagcctg ccaggcgtta ccgaggccgt tgttggggtc cgtcctctta
     1321 cgtcggtaag aagccagtaa gaaccggtaa gaagcgagta agaaaccgtt acggagtttt
     1381 gccgcggatt agttgccaac gccccttcgc cttgggtgta tcctagtaac cgagccttca
     1441 gttggaagac tcatgaccgt acacgccaac tcgcacgacc aagtgaagtg ataggagacc
     1501 ccccatggca ttgaccgaag tcatcgttct aatccctgaa gagcgcgtag gcgacttcta
     1561 ccgcgtggtc ggcgcgtgga tgggcggcgc caacctcgtc gacgaggcca acgccggccg
     1621 cagccgccgc cgttccgcaa gcagcagctc cacgccgctg gtgctgtccg atagcagccg
     1681 ctaccacccg ctccacgtcc acctggctgg cgtaagcaag gacatcaaga cctacgagat
     1741 gagcttcgac gagatcacga ccgtcatggg cgcgaggctg cccaagtccg ccgaggacca
     1801 ccgcgcctgg tgggccaaca cggagtcgca ctcgcaggcg ctcgcctgga tctcggccgg
     1861 ttggaaggtc gacggcgtcg acctcaacaa ccgcctcatc cacttcacgc ggccgaagta
     1921 agctcgtcgc gcagcggggg tctctcgtcg cggcctagcg gcggatgaaa cgtaggatca
     1981 cggcccggcg ccgcagagcg gcgccgggcc gtcgtctacg ctcccgccgc tagctgagcg
     2041 ctatgtcgga acctctcgga cgcctcgtct ggacggccgg agtggtacgg ccgtccagac
     2101 gccaacgctc aggcgtaccc aggccgcagc ctgtccaggt agagcttgcg gaacttcgcg
     2161 accttcggcg cgatcactgc ggcgcagtaa ggctgccgcg ggttacgcgc gaaatagtcg
     2221 tcgtggtact cctcggccgg gtagaagttc ttcaggggct cgagttcggt cacgagcggg
     2281 gcgccgtaga tgccggactc cccgatctcg cgcatcacgg cctccgcggt ctcgcgctgc
     2341 tggtcgtcaa ggtagaagac gacagagcgg tactgcgtgc cgatgtcgtt accctgcctg
     2401 tccttcgtcg tcgggtcgtg catggtgaag aagacgcgca ggatgtcggc gtaggagacg
     2461 acgctcgggt cgaactccag cctcacggcc tcggcgtgcc cggttgcccc ggtgcagacc
     2521 tgctcgtagc tcgggtcggc gacggtgccc ccggagtagc cggactgcac gctctcgatg
     2581 ccgttcaggc cgacgaagac tgcttccatg caccagaagc accccccggc cagcacggcg
     2641 gttgacgtct tgtcgctc
//
LOCUS       DAOQYE010000196         2658 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_42160_length_2658_cov_3.503265, whole genome shotgun sequence.
ACCESSION   DAOQYE010000196 DAOQYE010000000
VERSION     DAOQYE010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2658
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42160_length_2658_cov_3.503265"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /locus_tag="PKN52_04220"
     CDS             complement(<1..537)
                     /locus_tag="PKN52_04220"
                     /inference="COORDINATES: protein motif:HMM:NF014762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HNQ99179.1"
                     /translation="MAKERKASGEVGTNEDAVVAPTAADDVESAAAALQQKDDGATRL
                     SSSVGDYLKAIWLLAGDGVAGTGEIARELGVTAPSVTAMLSKMSGAELVAYSPYRGAS
                     LTPAGRREALRLVRRHLLLEMFMVTQLGFGWDEVHQEAELMEHVVSDVFTERLAKHLD
                     DPEFDPHGYPIPRADGSVP"
     gene            736..1188
                     /locus_tag="PKN52_04225"
     CDS             736..1188
                     /locus_tag="PKN52_04225"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNQ99180.1"
                     /translation="MPTSAAAPTTTLSPTEARNRLMLRARDAMDRLYAKPLDMPSLAR
                     SVGMSEAHFIRTFKAVFGETPYRYLQRRRVERAMFTLRQTSRSVTDVCFDVGFNSLGT
                     FSRTFKEIVGVSPSEYRMRYPPVPIPTCFFRAWARPSSFGEAPVGGAG"
     gene            1240..1647
                     /locus_tag="PKN52_04230"
     CDS             1240..1647
                     /locus_tag="PKN52_04230"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNQ99181.1"
                     /translation="MLKGINITNVLVLDQTEALEFYVGKLGLEVSNDVDFGFMRWLTV
                     KVPGSHGPDILLEVPAPPGMDPETAAQVREIVTKGATGFTVGFITTDAQAAFDDLKAK
                     GVQITDEPTRRDYGTDFGIRDPFGNHIRIVQYH"
     gene            1826..>2658
                     /locus_tag="PKN52_04235"
     CDS             1826..>2658
                     /locus_tag="PKN52_04235"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HNQ99182.1"
                     /translation="MSRLLPGVASIGDLRRRAKRRLPRMVFDYIDGGADNERTLRANV
                     AAYEDLVWRPRGAVKFDHVDLSTSVLGRQLPFPIILAPVGSSRMFWPHGEEAAAAAAG
                     EAGTIYTLSTLSGTRLEDVRAASKGVCWYQLYLCGGRDVAASAIERAKAAGYSALVVT
                     IDTAVAGMRERDIRDGAPQLIARKIPGMLPYVPQVLARPGWLLEFFQEGGAMSFPNVV
                     LPDGPMKYGDIGAQLAAAAVAWTDLDWIKELWDGPIIVKGVHVADDARRAVDAGASAV
                     VV"
BASE COUNT          446 a          928 c          880 g          404 t
ORIGIN      
        1 gggcacgctg ccgtccgccc ttggtatggg gtagccgtgc gggtcgaact ccgggtcgtc
       61 caggtgcttg gccagccgct cagtgaacac gtcggagacg acatgctcca tgagctcggc
      121 ttcctggtgg acctcgtccc agccgaagcc gagctgggtg accatgaaca tctcgaggag
      181 caggtgccgc ctcacgagcc gtagcgcctc gcgacgcccg gcgggtgtga gggaagcacc
      241 ccggtacggg gagtaggcga cgagctccgc cccgctcatc ttgctcagca tggccgtgac
      301 ggacggcgcc gtcacgccta gctcgcgcgc gatctcgccc gtgccggcca ccccgtcacc
      361 ggccagcagc cagatggcct tcaggtagtc gcccaccgag ctggaaaggc gggtggcgcc
      421 gtcgtccttc tgctgtagcg ccgcggcggc gctctcgacg tcatccgcgg cggtcggcgc
      481 cacgacagcg tcctcgttcg tcccgacttc accagacgcc ttgcgctcct tagccatgcc
      541 taaacgttag ggcagcgcag gcgggaacgc aactgggcgg gtcgtcgaag ttggcgcggt
      601 ccctcaggtc gtgctcgggg agtaaggccc gctcagggcg aacggcttgg gcggctacct
      661 gttcgtcggc ctggtatcgg gatgcgtcag atcgcaggcc ggaaagagcc cacgtccgct
      721 acccttagct catccatgcc gacgtcagcc gcagcgccca cgactaccct gtcgcccacg
      781 gaagcacgca accgcctcat gctgcgggcg cgcgacgcga tggacaggct ctacgccaag
      841 ccgctggaca tgccgagcct cgccaggagc gtcggcatga gcgaggcgca cttcatccgc
      901 accttcaagg ccgtgttcgg cgagacgccc taccgctacc tgcagcgcag gcgcgtcgaa
      961 cgcgccatgt tcacgctgcg gcagacgagc cgcagcgtca ccgacgtgtg cttcgacgtc
     1021 ggcttcaaca gcctcggcac gttcagccgc acgttcaagg agatcgtcgg cgtctcccca
     1081 tccgagtacc gcatgcgcta cccgcccgtc ccgataccga cgtgcttctt cagggcatgg
     1141 gcccgtccga gcagtttcgg agaagcgccc gtcggcgggg ccggctagag tcaggcgttc
     1201 gcgaaggcga aagctcagcg accactagga gggattacga tgctgaaggg cataaacatc
     1261 accaacgtgc tagtcctcga ccagaccgag gccctcgagt tctacgtcgg caagctcggc
     1321 ttggaggtga gcaacgacgt cgacttcggc ttcatgcgct ggctcactgt gaaggtcccc
     1381 ggtagccacg gccccgacat cctcctcgag gtgccggccc ctccgggcat ggatcccgag
     1441 accgccgccc aggtaaggga gatcgtgacg aagggcgcaa ccggcttcac ggtcggcttc
     1501 atcacgaccg acgcccaggc ggcgttcgat gacctgaagg ccaagggcgt gcagatcacg
     1561 gacgaaccga ccaggcgcga ctacggcacc gacttcggca tccgcgaccc tttcgggaac
     1621 cacatccgca tcgtccagta ccactgagga acggcacccg aaaggggact atcgacgagt
     1681 gcaccgcggt gcgcttgccg gcagcgggcg accgaccagc catcagggtc ggttgcccgc
     1741 tgctacatgg cggtagcatg cgacagccgt aagtacccgt tggagggcgc caagatgaca
     1801 gtttcgccag ccttaggatg cagcagtgag taggcttttg ccgggcgtag ccagcatcgg
     1861 cgacctgcgc cgcagggcca agcggcggct gccccgcatg gtgttcgact acatcgacgg
     1921 cggtgcggac aacgagcgca cactgcgcgc caacgtcgcc gcgtacgagg acctcgtctg
     1981 gcgcccgcgc ggcgcggtga agttcgacca cgtcgacctc agcaccagcg ttctcggccg
     2041 ccagctgccc ttccccatca tcctcgcgcc cgtcggctcc tcacggatgt tctggcccca
     2101 cggggaggaa gccgcggcag ccgcggcggg cgaggccggc acgatctata ccctctcgac
     2161 cctctccggc acacgcctgg aggacgtgcg cgccgccagc aaaggggtct gctggtacca
     2221 gctctacctc tgcggcggtc gcgacgtggc cgcgagcgcc atcgagcggg ccaaggccgc
     2281 aggctactcg gcgctcgtgg tgacgataga cacggccgtg gccggcatgc gcgagcgcga
     2341 tatccgcgac ggcgcgcccc agctcatagc gcggaagata cccggcatgc tgccctacgt
     2401 gccccaggtc ctcgcccgac caggctggct ccttgagttc ttccaggagg gcggcgccat
     2461 gagcttcccg aacgtcgtcc tgcccgacgg cccgatgaag tacggcgaca tcggcgccca
     2521 gctcgccgcc gcggcggtgg cgtggaccga cctcgactgg atcaaggagc tgtgggacgg
     2581 gccgatcatc gtcaaaggcg tccacgtcgc ggatgacgca cggcgagccg ttgacgcggg
     2641 cgcgagcgcg gtcgtcgt
//
LOCUS       DAOQYE010000197         2653 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_42278_length_2653_cov_2.827560, whole genome shotgun sequence.
ACCESSION   DAOQYE010000197 DAOQYE010000000
VERSION     DAOQYE010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42278_length_2653_cov_2.827560"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="PKN52_04240"
     CDS             <1..336
                     /locus_tag="PKN52_04240"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNQ99183.1"
                     /translation="DMPALMAAADVAVTKPGGLTSSEALSAGLPMVIANPYPLQEEVN
                     ASVLLEHGAAVRVDSISTLGHKLKRLLATPDRLAEMRANCARLAHPGSAAAVASCVLD
                     ELVLKGRAR"
     gene            342..2138
                     /locus_tag="PKN52_04245"
     CDS             342..2138
                     /locus_tag="PKN52_04245"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNQ99184.1"
                     /translation="MALVLVALAMAIGGPAAAQRVLGPSFPGGGGTAYFDVIVYGSEP
                     EGIAAAVAAAEEGARTLLVTADARLGGLFVLGELNMLDMRTQPFNYQRGLFQRWWRMV
                     GAMDAFDVLTAERAFQRLLSEAGVEVWTEAGQVAPRYVSGVLRGIELTDFEVEVNALQ
                     VVDASADADLAAQTDAPFDLGWERFGVSQRQADTLVLNVAGVDWQALRRGVSARGRSY
                     ANMRETVAWGSFGGVPTAYVPSQPDMRLRGLNLGLQNDGSVLVNALLLYGLDPLDPVS
                     LASGRARGLHEGEAIVAYLREHLPGFERARLAGAATELYVRESRHLLADCVLTADAAL
                     DNLVTTDAVAAGGYPLDAQSFTAHDAGFVWGAPDIYGGRLCMMTTATVDNLWVVGRSA
                     GYDPVAFASARVVPFGMAMGEAAGVAAATSVRLAVPTSMVVHDAGLLDSVREALVGRG
                     AYLPDAHARRATGPVDHPAYGAFRTLIARGLAVGGYDNDPKLDAPVSALSFAYLLSNV
                     ATRFFYRTDVGGDFVKLTLELVSDDAGAPLTSEIAGEVLTRAACELATCPPSPAWEDL
                     RIAGLAGGPEPQGGFTRGDSYRLAAALAREAP"
     gene            complement(2243..>2653)
                     /locus_tag="PKN52_04250"
     CDS             complement(2243..>2653)
                     /locus_tag="PKN52_04250"
                     /inference="COORDINATES: protein motif:HMM:NF012892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase C-terminal
                     domain-containing protein"
                     /protein_id="HNQ99185.1"
                     /translation="LLARRHWVAVVGYGTVLTAATLGAFAVALELLDATTAEAVTIAF
                     IGLALGQVWHVFNMREPGTPVLLNQLTRNPWVWAATLGCTALVLLAVALPPLRSALQV
                     EPLELSGWLLALGAGAAPVLVGQIGKAFGLGRVT"
BASE COUNT          388 a          948 c          939 g          378 t
ORIGIN      
        1 gacatgccgg ccctgatggc cgcggcggac gtggccgtca ccaagccggg cggcctcacg
       61 agcagcgagg ctctctcggc aggcctcccg atggtcatag ccaatcctta cccgttgcag
      121 gaagaggtga acgccagcgt gctactcgaa catggcgccg ccgtgcgcgt ggacagcatc
      181 tcgacgctcg gccacaagct caaacggctc ctcgccactc ccgaccgcct cgccgagatg
      241 cgcgccaact gcgcccggct cgcgcacccg ggttcggcgg cggccgtagc gagttgcgtg
      301 ctggacgaac tcgtcctcaa ggggcgcgca cggtgagggt catggctctg gtgctcgtcg
      361 ccttggcgat ggcgataggc ggacccgcag cggcgcagcg cgttctcggg ccgtcgtttc
      421 ccggcggtgg cggcaccgcc tatttcgatg tcatcgtcta cgggagcgag ccggagggca
      481 tagccgccgc ggtggcggcc gcggaagagg gcgcgcgcac gctattggtc acggccgacg
      541 cccgcctcgg cggcctgttc gtgcttggcg agctcaacat gctcgacatg cgcactcagc
      601 ccttcaacta ccagcgcggt ctgttccagc gttggtggcg catggtcggg gccatggacg
      661 ccttcgacgt gctcacggcc gagcgcgcct tccagcgcct gctctccgag gccggggtcg
      721 aggtctggac cgaggccggg caggtcgcgc cacgctatgt gagcggagtg ctgcgcggca
      781 tcgagctgac cgacttcgag gtcgaggtga acgcgttgca ggtcgtcgac gccagtgccg
      841 acgccgacct cgccgcccag accgacgccc ccttcgacct gggctgggaa cgcttcgggg
      901 tctcccagcg ccaagccgac acgctggtcc tgaacgtggc gggcgtggac tggcaggcgc
      961 tcaggcgagg cgtcagcgcg cgcggccgga gctacgccaa catgcgcgag acggtggcct
     1021 ggggctcctt cggcggcgtg ccgacggcct acgtccccag ccagccggac atgcgcctca
     1081 gggggctcaa cctcggcctg cagaacgacg gcagcgtcct ggtgaacgcc ctcctcctct
     1141 acggactcga tccgctcgat ccggtcagcc tggcgagcgg gcgcgcccgc ggcctccacg
     1201 agggcgaggc gatcgtcgcc tacctccgcg agcacctgcc aggcttcgag cgggcgcgct
     1261 tggcaggcgc cgctaccgag ctctacgtgc gcgagtctcg ccacctcctc gccgactgcg
     1321 tcctcaccgc ggacgccgcc ctcgacaacc tggtgacgac ggacgcggtg gccgcgggcg
     1381 gctacccgct cgacgcccag agcttcacgg cgcacgacgc cggcttcgta tggggcgcgc
     1441 ccgacatcta cggcggccgg ctttgcatga tgaccacggc gacggtcgac aacctgtggg
     1501 tggtagggcg cagcgccggc tatgacccgg tcgcgttcgc ctcagcacgc gtcgtgccct
     1561 tcgggatggc catgggagag gcggcagggg tcgcggccgc gacatccgtg cgtctcgccg
     1621 taccgaccag catggtggtc cacgacgccg ggctgctcga cagcgtgcgg gaggcgctcg
     1681 tcggtcgcgg tgcgtaccta ccggatgccc acgcgcgccg cgcgaccgga ccggtcgatc
     1741 atccggccta cggcgctttc cgcacgctca tcgcgcgcgg cctggccgtg ggaggctacg
     1801 acaacgaccc gaaactggac gcccctgtgt cggcgctctc cttcgcctac ctgctgagca
     1861 acgtggccac gcggttcttc taccggaccg acgtcggcgg cgacttcgtg aagctgacct
     1921 tggagttggt gtccgacgac gccggagcgc ccttgacgtc ggagatcgca ggcgaggtgc
     1981 tcacacgggc cgcgtgcgag ctagccacgt gcccaccgag ccccgcctgg gaggacctgc
     2041 gtatcgcagg gctggccggc ggaccagagc cgcaaggcgg cttcacccgt ggcgacagtt
     2101 accggctcgc ggcggctctg gcccgcgagg ccccctagcc agagccgcga actcgctcgc
     2161 tggaactcgc gcgctgccgg tgctaccgcg aacgtgcgtg cgcgggacga gcataggcgc
     2221 gagagacgag cgtaggcggc cgtcaggtca cccgcccgag gccgaaggcc ttgccgatct
     2281 ggccgacgag cacgggcgcc gcccccgcac cgagggccaa gagccagccc gacagctcga
     2341 gcggttcgac ctgtagagcg gacctgaggg gcgggagcgc cacggcgagc agcacgagcg
     2401 cggtgcagcc cagcgtcgcg gcccagaccc acgggttgcg cgtgagttgg ttcagtaaga
     2461 ctggcgtccc tggctctcgc atgttgaaca cgtgccagac ctgaccgagc gccaagccga
     2521 tgaacgcgat ggtcaccgcc tccgccgtgg tggcgtcaag cagctcgagg gccaccgcga
     2581 aggcgccgag ggtcgccgcg gtgagcaccg tcccgtaacc gacgacggcc acccaatgtc
     2641 gtctcgcgag gag
//
LOCUS       DAOQYE010000198         2649 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_42376_length_2649_cov_2.666924, whole genome shotgun sequence.
ACCESSION   DAOQYE010000198 DAOQYE010000000
VERSION     DAOQYE010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2649
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42376_length_2649_cov_2.666924"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            246..>1012
                     /locus_tag="PKN52_04255"
     CDS             246..>1012
                     /locus_tag="PKN52_04255"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HNQ99186.1"
                     /translation="MRRALIAIAALVLVAFGLSQAGIDWGRAAVRDLGEVGVETGFPD
                     GSFLGEGPVTGYQAAVLVDRLLTRVDTVTGCTDAGAGVPDPTFRFVDVSPDHWAAGAA
                     QRIAALGVGQAFPDGRLNGDEYLSGYQVALLIDQAVNVVDAKVACGTQGVQEKLGAMS
                     AEVAALRADVAAGALTGPAGPTGPQGPAGPAGPQGPQGDRGEAGSAGTDGAPGPAGPA
                     GPVGPAGAQGPQGERGAPGPAGRDGAAGPAGPSGAAGL"
     assembly_gap    1013..1022
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1023..1293)
                     /locus_tag="PKN52_04260"
     CDS             complement(<1023..1293)
                     /locus_tag="PKN52_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99187.1"
                     /translation="MRTRGAGIALVALGSCLPARAGRTSRTDRTGGASKATQPGWTRR
                     AALTLRTGGTGGSGRAGRTLRTDAVEGVADAIQVHVLVQSETSIAV"
     gene            complement(1653..2483)
                     /locus_tag="PKN52_04265"
     CDS             complement(1653..2483)
                     /locus_tag="PKN52_04265"
                     /inference="COORDINATES: protein motif:HMM:NF014674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99188.1"
                     /translation="MPSQYWALGASALPLWVLVKASNAVAASLTFETKRQAQLLEAGR
                     RVVQETRGWNEGGQRTYSLRSKETAADYRYLRDPDIPALPIPEERKRATLATMPELPK
                     ARLTRYLAAGVRAMEAEQIAYDQPAARLFDATVAVYGGPAQNVANWLTGEVAGRANAL
                     GKGVGDTGLTPVALARLLELVDDGTLSTTAAKDLVQDLLTGADPAELVASRGLGQVSD
                     ASALGALVAEVLEANHELVERVRSNPKAINALLGKVMAASRGKANPDLARRLLEERLR
                     "
BASE COUNT          360 a          916 c          997 g          366 t
ORIGIN      
        1 ggcctgcgcc atggcgccga cggcaagggc gaccgccacc agggtcgcga gtatgcgagt
       61 tagatagttc acctggccag atgatagccg gccgttcgcc cccgtgaccc cgacggcgcc
      121 tcgcaggggg tacgaggcgt gatatgcacg gtgcgtggag gcgtgtggat gggcgattat
      181 ctagtccagc ggcgatcggc ttcgaaccag cggggttctg ggccgcgatg cccggtagga
      241 gagagatgag aagagcactc attgccatcg cagcgctcgt cctggtggcc ttcggcctca
      301 gccaggccgg gatcgactgg ggtagggccg ccgtcaggga cctgggcgag gtcggggttg
      361 agaccggctt ccccgacggc tcgttcctgg gagaaggccc ggtgaccggc taccaagccg
      421 ccgtgctcgt cgatcgcctg ttgacgaggg tcgacaccgt caccggctgc acggacgccg
      481 gcgccggggt gccggacccg actttccgtt tcgtggacgt gagccccgac cactgggcgg
      541 ccggcgccgc ccagcgcata gccgcgctag gcgtgggcca ggctttcccg gacggccggc
      601 tgaacggaga cgagtacctg agcggctatc aagtggccct gctgatcgac caggcggtca
      661 acgtggtaga tgcgaaggtc gcttgcggaa cacagggtgt gcaggagaag ctcggcgcca
      721 tgagcgccga agtggccgcg ttgcgtgccg acgtcgctgc gggcgccctt accggcccgg
      781 ctggcccgac cggacctcag ggtccggctg gcccggccgg tcctcagggt ccgcagggcg
      841 accgcggtga ggctggctcg gccggcaccg acggcgcgcc cggtccggcc ggacccgccg
      901 gtcccgtcgg tcccgccggc gcgcagggtc cgcaaggtga gcgcggtgcg cccggtccgg
      961 ctggccgcga cggcgccgcc ggtccggctg gtccgagcgg cgcggcaggg ctnnnnnnnn
     1021 nncgacggcg atggacgtct cggactggac gaggacgtga acctggatgg cgtcggcgac
     1081 gccctcgact gcgtcggtcc gcaaggtcct gccggcgcgg ccggacccgc cggtcccgcc
     1141 ggtccgcagg gtgagcgcgg cgcggcgggt ccagccgggc tgcgtggcct tactggcgcc
     1201 gccggtcctg tcggtccggc tggtcctgcc ggcccgcgcg gggaggcagg agccaagggc
     1261 gacaagggcg ataccggcgc cacgggtccg caaggtccgc agggtgctcc tggtgccacg
     1321 ggggccgtcg gggcggtcgg tccgcaaggt cctgccggcc ccgcggggcc gatgggacca
     1381 accggaccga tcggaccgga aggccccgct ggcgcgccag gcgcccaggg tccggcaggg
     1441 ccggcaggcc ccgccggacc cgcgggtccc gctggcccgg ccggaccgga aggcccgcag
     1501 ggcccagtgg gtccggaagg acctccggga cctccggggt gatcgcggac ccgccacgtc
     1561 gcgggccgta ctacatgctt cgctagacgc gggatgcccc cgagcaccac tcgggggcat
     1621 cccgctgttt ggcggctcgc tcgactaccg gcttagcgga ggcgctcttc caacaggcgc
     1681 cgtgccaggt cggggttggc cttgccccgg ctggctgcca tgaccttccc gagcagggcg
     1741 ttgatcgcct ttgggttgga cctgacgcgc tcgacgagct catggttggc ctccagtacc
     1801 tccgccacga gcgctcccaa ggcggaggcg tcactgacct gaccgaggcc gcggctggcc
     1861 accagctcgg ccgggtcggc gcccgtgagc aggtcctgaa ccaggtcctt ggccgcggtg
     1921 gtggagagcg tgccgtcgtc gacgagctcg aggaggcgcg caagggctac gggcgtcagg
     1981 ccggtatcac cgacaccctt gcccagagcg ttagcgcgac cggcgacctc gccggtcaac
     2041 cagttagcca cgttctgcgc gggaccgccg tagacggcga cggtagcgtc gaacaggcgc
     2101 gccgcgggct ggtcgtaggc gatctgctcg gcctccatgg cgcgtacgcc cgccgcgagg
     2161 tagcgcgtca ggcgtgcctt gggcagctcg ggcatggtcg ccagcgttgc gcgcttgcgc
     2221 tcctccggga tcgggagcgc cgggatgtcc gggtcgcgta ggtaccggta gtcggcggcg
     2281 gtctctttgg agcgcagcga gtaggtgcgt tggccgccct cgttccagcc gcgcgtctcc
     2341 tggacgacgc ggcgcccggc ctccaggagc tgagcctgac gcttcgtctc gaaggtcaag
     2401 gaggccgcca cggcattcga cgccttcacc aagacccaca gtggcagcgc gctggccccg
     2461 agcgcccagt actggctggg caacgcgcac tatgcgctgc gcgactgcaa gaaggcgatc
     2521 gacgcgcagc gtctgctgct cgccacctgg ccggaaagcc cgaaggcccc cgacgccctg
     2581 atcaacatcg ccacctgcca gcaggaactg ggcgacgcca agggcgcccg ctcgacctac
     2641 gagaacatc
//
LOCUS       DAOQYE010000199         2646 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_42463_length_2646_cov_2.745658, whole genome shotgun sequence.
ACCESSION   DAOQYE010000199 DAOQYE010000000
VERSION     DAOQYE010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2646
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42463_length_2646_cov_2.745658"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="PKN52_04270"
     CDS             complement(<1..564)
                     /locus_tag="PKN52_04270"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HNQ99189.1"
                     /translation="MLRRLAALTFTLLLLAPSALATAYPVTVVDDLGREVTLTAAPAR
                     IVSMLPSHTESVCAVDACGSLVGVDTYSNYPASVTELPTLGDAFGPNLEAIVALEPDF
                     VLVDEYSGLQVPLEALGIPVYAGSPQTIAETYDFLVTLGTLLDRETEAAVLVGRLQGE
                     LAGVASVLAKVHGPTVFVELDPTPYSVG"
     regulatory      complement(672..821)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            839..1924
                     /gene="zapE"
                     /locus_tag="PKN52_04275"
     CDS             839..1924
                     /gene="zapE"
                     /locus_tag="PKN52_04275"
                     /inference="COORDINATES: protein motif:HMM:NF040713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HNQ99190.1"
                     /translation="MTHVLTVDQLEPPPPPSVRDLRPPPRFAGVAFETYAPQDPTQAA
                     ARDRLQEFVLGRRWRHEVPGPDAGRPGRPVLQARALRWPWQRPSPQAGAGLYLDGGFG
                     VGKTHLLAAAYAAADTSAKRYLSFQELVYLIGVMGMARAQTEFATSDLLCIDEFELDD
                     PGNTLIVKTFLAASFAAGTAVVTTSNTPPAAQGQGRFNASDFKREIQSLAASFEVVPV
                     DGADFRQRLRQGSWLTEAELTSRLQSEASPSPRLAVTWDELLELLRSMHPTRFGGVLA
                     QVGSVYLSGVRTLASQNDALRFVHFIDKLYDRQVMLRASGDGPLIDVFDPSYREGAYA
                     KKHHRCASRLAELLGEEVGERTGRIAV"
     gene            complement(1939..>2646)
                     /locus_tag="PKN52_04280"
     CDS             complement(1939..>2646)
                     /locus_tag="PKN52_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99191.1"
                     /translation="DRVYARDAATAAHLLGRGVSGAAYVGNPMMDGLEAAPLVPYELR
                     GRPTVALLPGTRAWAARSVAPMIAALERLPGALGLVAWTLGQTPAPPPGWENDLAPVP
                     GVAVAWRRGPNRLWWVAERFAAVLASADVALGTSGTANEQAVGLSVPTVAFPFPPHLS
                     GAFVANQARLLGAGLTVGEADPRALAAHVARLWRDPAARERTAAAARERMGSSGGSAA
                     LAGELADWLGALPKRDG"
BASE COUNT          396 a          924 c          971 g          355 t
ORIGIN      
        1 gcccacggag tacggggtcg gatcgagctc gacgaacacc gtcggtccgt gcaccttcgc
       61 gaggacgctc gcgacgccgg cgagctcgcc ctgcaagcgg cctaccagga cggcggcctc
      121 ggtctcccgg tcgaggaggg tgccaagggt gacgaggaag tcgtaggtct ctgcgatggt
      181 ctggggggag ccggcgtaga ccggtatgcc gagcgcctcc agcgggacct ggaggccgct
      241 gtactcgtcc accaggacga agtccggttc caacgcgacg atggcctcga ggttcggacc
      301 gaaggcgtca ccgagggtgg gcagctccgt cacgctagca gggtagttgc tgtaagtgtc
      361 cacccccacg agcgagccgc aggcgtcgac ggcgcagacg ctctccgtgt ggcttggcag
      421 catgctcacg atgcgcgcgg gtgcggcagt caacgtgact tcgcgcccga ggtcgtcgac
      481 cacggtgacc gggtaggcgg tggcgagggc gctcggtgcg agcaggagga gcgtgaacgt
      541 gagtgccgcg aggcgtcgga gcatggagcc cttccttccg gcctgcggtt cggaggcgtc
      601 tgatcgactg gaggggcggc cgcgcctgat cgacggaagg gcgctacctg cctccctcgt
      661 ccgcgcaagg ggtcaagtga aggttcgccg gccggtgttc ggactcgggc gccaccaagc
      721 gcgcccttac cgttgcggga cagtgccgga ctctcaccgg acttccccga tcggcgacca
      781 agggaactct acggcgcgcc accgggcaag tcaaacggcg ggtggtaggc tcccgtgcgt
      841 gacgcacgtg ttgacggtgg accaactgga gccccctccc ccaccgtcgg tacgcgacct
      901 gcgtccgccg ccgcgcttcg ccggggtggc gttcgagacg tacgccccgc aggaccccac
      961 gcaggcggca gcgcgcgacc ggctgcagga gttcgtgctc ggcaggaggt ggcggcacga
     1021 agtgccggga cccgacgcag ggcgccccgg acggcccgtc ctgcaggcgc gcgccttgcg
     1081 ctggccttgg cagcgtccgt caccccaggc cggcgcaggc ctgtacctcg acggcggctt
     1141 cggggtcgga aagacgcacc tcctcgcggc cgcgtacgct gccgccgaca cgtccgcgaa
     1201 gcgttatcta agcttccagg agctcgtcta cctgatcggc gtgatgggga tggcgcgggc
     1261 gcagacggag ttcgcgacga gcgacctcct atgcatcgac gagttcgagc tcgacgaccc
     1321 cggcaacacg cttatcgtca agaccttcct ggccgcgagc ttcgcggccg gcaccgccgt
     1381 ggtcacgacg tcgaacacgc cgcccgcggc gcagggccag gggcgtttca acgcctcgga
     1441 cttcaagcgc gagatccaat cgctggccgc ctcgttcgag gtcgtcccgg tggacggcgc
     1501 cgacttcagg cagcggctca ggcagggcag ttggctgacc gaggcggagt tgacgagccg
     1561 tctgcagagc gaggcgtcgc catcaccccg cctggccgtg acgtgggacg agctcctgga
     1621 gttgctgcgc agcatgcacc cgacgcgctt cggcggcgtg ctcgcccagg tcggcagcgt
     1681 ctatctaagc ggggtacgca cgctcgcctc ccagaacgac gccctgcgct tcgtgcactt
     1741 catcgacaag ctctacgacc ggcaggtcat gctgcgcgcc agcggcgacg gacccctgat
     1801 cgacgtcttc gacccgagct accgcgaggg cgcctacgcc aagaagcatc accgctgtgc
     1861 ctcgcgcctg gctgaactcc tcggcgagga agtcggcgag cgcacggggc gtatcgcggt
     1921 ctgacggacg actagcgctc agccgtcgcg cttgggcagg gcccctaacc agtccgcgag
     1981 ctcaccggcc aacgcggcgc tccccccgga cgaccccatg cgctcccgtg cggccgccgc
     2041 ggtgcgctcg cgggcggcag ggtcgcgcca gagccgcgcc acgtgagcgg cgagggccct
     2101 cgggtcggcc tcgccgacgg tgaggccggc gccgagcagg cgcgcctggt tggcgacgaa
     2161 ggcgccggag aggtgcggtg ggaaggggaa cgcgacggta ggcacactta gaccgaccgc
     2221 ctgctcgttg gcggtcccgg acgtgccgag agcgacgtcg gcgctggcga gcacggcggc
     2281 gaagcgctcg gccacccacc aaagccggtt ggggccgcgg cgccacgcga ccgcgacccc
     2341 aggtacgggc gccaggtcgt tctcccaacc tgggggcgga gcgggcgtct gcccgagcgt
     2401 ccacgccacc aggccgaggg cgccgggcaa ccgctcgagt gccgcgatca tgggcgccac
     2461 gctgcgagcc gcccacgccc gcgtgccggg cagcagggcg acggtcggtc ggccgcgcag
     2521 ctcgtacggc acgagaggcg cggcctcgag cccatccatc atggggttgc cgacgtaggc
     2581 ggcgcccgag acaccgcgtc cgagcaggtg agcggccgtc gccgcgtcac gggcgtagac
     2641 ccggtc
//
LOCUS       DAOQYE010000200         2641 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_42568_length_2641_cov_2.816705, whole genome shotgun sequence.
ACCESSION   DAOQYE010000200 DAOQYE010000000
VERSION     DAOQYE010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2641
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42568_length_2641_cov_2.816705"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="PKN52_04285"
     CDS             <1..223
                     /locus_tag="PKN52_04285"
                     /inference="COORDINATES: protein motif:HMM:TIGR02276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99192.1"
                     /translation="DTTTNQIVNTIKENMGRRPWGIAITPDGKTVFTANGLSDSVSVI
                     DTACGCVVKNIPAGRGAHSAEIGVIPGGL"
     gene            213..1298
                     /locus_tag="PKN52_04290"
     CDS             213..1298
                     /locus_tag="PKN52_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99193.1"
                     /translation="MVSRLFRGTAVSLVVAFIATAFAQYGYQPFFEQPLIQSRAYGME
                     LILVRLRAIDQFDAPLDDDLMELIELIGESDVNRFIGTLRAADPELADELLDEIEELE
                     EKAEEGRNHHGSVREVRELTERAYDLIIPSQVRKSPAFLAALIAYLTLGEGGIAEGYE
                     EAIGDEEPFLFTGGWSSLQSVKELWSQLTIYATPAQAADVNEMLAQLDDIYFSPEPPE
                     PLVGNPEEAEAPAQRMLGLIEPIADAEVFAGRDMQALAVSLVATQAQACRAYDAGNDE
                     VGLEGVYAVGEMYARYLAGFLGFMAPDVHEEAAETIYALTGIQDEGAEDEEDEDDEDE
                     RPEVADPAGACRELLEALEEAVEVLGG"
     gene            1399..2325
                     /locus_tag="PKN52_04295"
     CDS             1399..2325
                     /locus_tag="PKN52_04295"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNQ99194.1"
                     /translation="MSPTGSATTVMGGPGDDQAAILADRLGRRYGDVTAVESVSFSIH
                     PGEFFALLGPNGAGKTTTLHMLTTMVRPTSGTASVFGFDVVREAHAVRRLLGMVFQDP
                     ALDDRLTARENLEIHAVLYGIGRKERAGVIERALDWAALTEARDRRVRSFSGGMKRRL
                     ELARALTHSPKVLFLDEPTIGLDPQGRRNLWEHIAALREGGMTVLMTTHNLPEAEACD
                     RVAIMDAGRLVAVGTPPELVARFAGGHSDQPPTAALAGRAASGMEAAEPAKPADLEDV
                     FIALTGKQLRDEQATARDRLVSFAKRGGEHTR"
     gene            2322..>2641
                     /locus_tag="PKN52_04300"
     CDS             2322..>2641
                     /locus_tag="PKN52_04300"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ99195.1"
                     /translation="MSANTRASGAFGATAVGIRSWAGVAPSVVLVMWRREMKRYLRDR
                     SQIFGGFSRTVLWLVILGYGLGASLREIEGYTYGQYILPGVIVLNVLFAALQCAIALV
                     WDREV"
BASE COUNT          431 a          929 c          885 g          396 t
ORIGIN      
        1 cgacacgacc accaaccaga tcgtcaacac gatcaaggag aacatggggc ggcggccctg
       61 gggcatcgcc atcaccccag acggcaagac ggtcttcacg gccaacggcc tgtccgacag
      121 cgtcagcgtc atcgacacgg cctgcggctg cgtcgtcaag aacatccctg ccggtcgagg
      181 tgcccactcg gccgagatcg gagtgatccc cggtggtctc taggctcttc cgcggtaccg
      241 ctgtctcgct cgtagtggcc ttcatcgcca cggcgttcgc ccagtacggc taccagccgt
      301 tcttcgagca gcccctcatc cagagccgcg cgtacggcat ggagctcatc ctcgtgcggt
      361 tgcgcgccat cgaccagttc gacgcgcccc tcgacgacga cctcatggag ctgatcgagc
      421 tcatcggcga gagcgacgtc aaccgcttca tcggcaccct gcgcgccgcc gacccggagt
      481 tggccgatga actgctcgac gagatcgagg aactcgagga gaaggccgag gagggcagga
      541 accaccacgg ctccgtccgc gaggtccgcg agctcacgga gcgcgcttac gacctgatca
      601 tcccgtccca ggtccgcaag tcaccagcct tccttgcggc actgatcgcc tacctgacct
      661 tgggtgaggg cggcatagcc gaggggtacg aggaagccat cggcgacgag gagccgttcc
      721 tcttcacggg cggctggtcg tcattgcaga gcgtgaagga gctgtggtcg cagctgacca
      781 tctacgccac gcccgcgcaa gccgccgacg tgaacgagat gctcgcccag ctcgacgaca
      841 tctacttctc tcccgagccc cccgagcccc tcgtcggcaa ccccgaggag gccgaggctc
      901 cggcgcagcg aatgttgggc ctgatcgagc ccatcgccga cgccgaggtg ttcgccggtc
      961 gcgacatgca ggccctggcc gtaagcctcg tggcgacgca ggcgcaggcc tgccgtgcct
     1021 acgatgcggg taacgacgag gtcggcctcg agggcgtgta cgccgttggc gagatgtacg
     1081 cccgctacct ggccgggttc ctcggcttca tggcccccga tgtgcacgag gaggcggccg
     1141 agaccatcta cgccctcacc ggcatccagg acgagggagc cgaggacgag gaggacgagg
     1201 acgacgaaga cgaacgtccg gaagtcgccg accccgccgg cgcctgcagg gaactcctcg
     1261 aggcgctcga ggaagccgta gaggttctgg gaggctgaga gccgatgcgt gtggcgctgg
     1321 ggtgcgtccc ggtctctcac gcgaacgctc gcaggtcctt gaccaccttg cgaggatgag
     1381 cgcggtactc tccacgggat gagcccaacc ggcagcgcca ccactgtcat gggcggaccc
     1441 ggggacgacc aagcggccat actcgcagac cgactcggcc gccgttacgg agacgtgacg
     1501 gcggtcgagt cggtctcgtt ctccatccac cccggcgagt tcttcgccct cctcggcccc
     1561 aacggagccg gcaagacgac gacgttgcac atgcttacga ccatggtcag gccgacgtcc
     1621 ggcacggcgt ccgttttcgg tttcgacgtc gtgcgcgagg cccacgctgt gaggcgcctc
     1681 ctcggcatgg tcttccagga ccctgccctc gacgatcgcc tcacggccag ggagaacctc
     1741 gagatccacg cggtgctgta cggcatcgga cgcaaggagc gcgcgggcgt catcgagcgc
     1801 gccctcgact gggcggcgct cacggaggcg cgcgaccggc gggtgcggtc gttctccggc
     1861 ggcatgaagc gccgcctgga gctcgcccgc gcgctcacgc actcgcccaa ggtcttgttc
     1921 ctcgacgagc cgaccatcgg cctcgacccg caaggtcggc gcaacctgtg ggagcacatc
     1981 gccgcgctgc gcgagggcgg catgactgtc ctcatgacca cccacaacct cccggaggcc
     2041 gaggcctgcg accgcgtcgc gatcatggac gcaggccggc tcgtggccgt cgggacgccg
     2101 cccgagctag tggcgcgctt cgcgggaggg cactctgacc agccgcccac cgccgcgctc
     2161 gcgggccgag cggcgagcgg gatggaagcg gcagagccgg cgaagcccgc cgacctcgag
     2221 gacgtgttca tcgccctcac cggcaagcag ttgcgtgacg agcaggcgac ggcgcgcgac
     2281 cggctggtga gcttcgccaa gcgcggcggg gagcacacgc gatgagcgcc aacaccagag
     2341 cctcgggggc cttcggggcc accgccgtcg gcatcaggtc ttgggcgggc gtagctccct
     2401 ccgtcgtgct cgtcatgtgg cgccgcgaga tgaagcgcta cctccgcgac cgctcgcaga
     2461 tcttcggcgg cttctcgcgg accgtgctct ggctcgtcat cctcggctat ggcctcgggg
     2521 cgtcgctacg ggagatcgag ggctacacgt acggtcagta catcctcccg ggcgtcatcg
     2581 tgctcaacgt gctcttcgcg gcgctccagt gcgccatcgc gctcgtgtgg gaccgcgagg
     2641 t
//
LOCUS       DAOQYE010000201         2640 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_42588_length_2640_cov_3.264217, whole genome shotgun sequence.
ACCESSION   DAOQYE010000201 DAOQYE010000000
VERSION     DAOQYE010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42588_length_2640_cov_3.264217"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="PKN52_04305"
     CDS             complement(<1..263)
                     /locus_tag="PKN52_04305"
                     /inference="COORDINATES: protein motif:HMM:NF012354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNQ99196.1"
                     /translation="MPGSPDADQLLTLLAVAEAGNESAAAELLGIGQSSVSRRLAALQ
                     KLSEAPLTQRTVGGTRLTPAGNALLGPARALRATLLEAESLLDP"
     gene            511..1932
                     /locus_tag="PKN52_04310"
     CDS             511..1932
                     /locus_tag="PKN52_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNQ99197.1"
                     /translation="MYFQQFYDHDLAQGSYLIGCQATGEAIVVDPRRDVDVYLETAAA
                     EGMRIVAVTDTHIHADYVSGARELAAAAGAGLYLSDEGGPDWRYAFEHRGLSHGSVIR
                     VGNVALEALHTPGHTPEHLAFLVTDGARSPLPSHLLSGDFVFVGDVGRPDLLDAVAGG
                     EGTRFEGARRLFASLRDRFLSLPDHVQVWPGHGAGSACGKALGAVASSTVGFERLTAW
                     WAHYLASGDEVGFAAALLDGQPDAPTYFGRMKRVNRDGPPILGDRGRLRSFAPDELRG
                     RVNRDIVLVDARTLAEQRSGTVPGALTVPGGASFATYAAWVIDPDVEKRDIVLLTAGA
                     ERAAQLADRLIRVGIDGTTGYVTALEGLETAPLPVLRLGSDSLAGADVLDVRTASEYA
                     AKHLPGARQLAAGNVLARLHELPRGRKLVVHCQGGGRAAAVASALRNRGFANVVELGG
                     TLDVWLQHVGNAVGADAEGVSAP"
     gene            1929..>2640
                     /locus_tag="PKN52_04315"
     CDS             1929..>2640
                     /locus_tag="PKN52_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99198.1"
                     /translation="MTEQTGRPSPRGGNWWPLALIAVGVVILVGNLGLGLGPVWRFLG
                     NLLAYWPVALIAIGADMLTRGRYRLLIASIAIGVVVVLAAYGSKGPETISIREAVGGA
                     TSARVVLDVGVSPLRLNTAQGGDLIRGTVSQAAGERVEHSASLRNGRAEVTIRARQRG
                     WLPFSWGGGRGGLWDLTLARNVPTELVIDGGVGNIELDLSEARLTSLDVDSGVGSLTL
                     TLPRSGGFQGEIDGGVGSV"
BASE COUNT          367 a          914 c          969 g          390 t
ORIGIN      
        1 ggatccagca gagactcggc ttccagtagc gtcgccctga gcgcacgcgc cgggccgaga
       61 agggcgttgc cggcgggcgt gaggcgtgtg ccgccgaccg ttcgctgcgt caggggagcc
      121 tccgagagct tctggagcgc cgcgagcctg cggctgacgg aagactggcc gatcccgagc
      181 agctcggccg ccgcgctctc gttgccggcc tcggccacgg ccaggagtgt caggagctga
      241 tcggcgtctg gtgatcccgg catggcacca ttatgcacgc agcgcatacg gaggcgccct
      301 gcggccggag cgcgtggtgg cgtgaccgtg cgtcggcggc gtcgtgactg accgccaggc
      361 attaacctgt ccttgaagcc gcgcgttcac gagcgagcga gttcgctgtg aacggcttcg
      421 ctcgtgaacg gcttcgctgg agagcgagcg ggctcgatca aagccgtaga cccgtctggc
      481 acgagcgatc cgcggcgtgg aggggcgaga gtgtacttcc aacagttcta cgaccacgac
      541 ctcgcgcagg gcagctacct gatcggctgt caggcgaccg gtgaggcgat cgtcgtcgac
      601 ccgcgccgcg acgtcgacgt ctacctcgag accgccgcgg ccgagggcat gcgcatcgtc
      661 gcggtgaccg acacgcatat ccatgccgat tacgtctccg gcgccaggga gttggcggcg
      721 gccgccggcg ccgggctcta cctgtcggac gaaggcggcc cggactggcg ttacgctttc
      781 gagcatcgcg gtttgtcgca cggaagcgtc ataagggtgg gcaacgtggc gctagaggcg
      841 ttgcacacgc ccggccacac gccggagcac ctggcgttcc tcgtgacgga cggcgcgcgc
      901 tccccgctgc cgtcccacct cctcagcggc gacttcgtgt tcgtagggga cgtcgggagg
      961 ccggacctgc tcgacgccgt cgccggcggc gaaggcactc gcttcgaggg cgccaggcgc
     1021 ctcttcgcca gcctccgcga ccgcttcctg agcctgccgg accacgtgca ggtctggccg
     1081 ggccacggcg ccggcagcgc ctgcggcaag gccctcggcg cggtggcgag cagcacagtc
     1141 ggcttcgagc gcctcacggc ctggtgggcg cactacctgg ctagtggcga cgaggtcggt
     1201 ttcgcggccg cgctcctcga cggccagccg gacgccccga cctacttcgg acgcatgaag
     1261 cgcgtcaacc gcgacggacc gcccatcctc ggcgaccgcg ggcggctgcg cagcttcgcc
     1321 cccgacgagc ttcgcggtcg ggtgaacagg gacatcgtcc tggtcgacgc gcgcacgctc
     1381 gcggagcagc gctccggtac cgttccgggt gccctgaccg tgcccggcgg cgcgtccttc
     1441 gccacctacg ccgcctgggt catcgatccg gacgtcgaga agcgcgacat cgtcctcctg
     1501 accgccggcg ccgagcgcgc cgcgcagctg gccgaccggc tgatccgcgt cggcatcgac
     1561 gggaccacgg gttacgtgac cgcgctggag ggtctcgaga ccgcgccgct gcccgtgtta
     1621 aggctcggca gcgactccct ggccggggcg gatgtgctgg acgtgcgcac cgcgagcgag
     1681 tacgccgcca agcacctccc cggcgccagg cagctcgcgg ccggcaacgt gctggcgcgg
     1741 ctgcatgagc tgccgcgcgg ccggaagctc gtcgtccact gccaaggcgg cggccgggcg
     1801 gcagcggtcg ccagcgcgct gcgcaaccgg ggcttcgcca acgtcgtcga gttaggcggc
     1861 accctggacg tgtggctcca gcacgtcggg aacgcagtgg gtgccgatgc ggaaggggtg
     1921 agcgcgccgt gaccgaacag accggaagac cctcgccgcg cggcggcaac tggtggccgc
     1981 ttgccctgat cgccgtcggg gtcgtgatac tcgtgggcaa cctcggtctg ggcctcggcc
     2041 ctgtctggcg tttcctaggc aacctgctcg cctactggcc cgttgcgctc atcgccatcg
     2101 gggcggacat gctcacgcgc ggccggtacc ggttgctcat cgcgtcgatc gccatcgggg
     2161 tggtcgtcgt gctcgccgcc tatgggagca agggcccaga gaccatctcc atccgcgagg
     2221 cggtcggcgg ggcgacgagc gctcgggtcg tgctcgacgt cggcgtcagc ccgctgcgcc
     2281 tgaacactgc gcaaggcggc gacttgataa gaggcaccgt gagccaggcg gccggcgagc
     2341 gggtcgagca ctcggcctcg ctccgcaacg gccgcgccga ggtgaccatc agggcgcgcc
     2401 agcgcggctg gttgccgttc tcctggggcg gcggtagagg cggcctctgg gacctaacgc
     2461 tcgctcggaa cgtaccgacc gagctggtga tagacggcgg ggtaggcaac atcgagctgg
     2521 acctgagcga agcgcgtctg acgagcctcg atgtggactc cggggtgggg agcctcaccc
     2581 tgacgctgcc gcgttctggc ggcttccagg gcgagatcga cggcggggtc gggagcgtca
//
LOCUS       DAOQYE010000202         2638 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_42636_length_2638_cov_2.779714, whole genome shotgun sequence.
ACCESSION   DAOQYE010000202 DAOQYE010000000
VERSION     DAOQYE010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2638
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42636_length_2638_cov_2.779714"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1711)
                     /locus_tag="PKN52_04320"
     CDS             complement(<1..1711)
                     /locus_tag="PKN52_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99199.1"
                     /translation="MRLRALLGLSLVALILAACGGTPTPPPPALGWVEISGGGFLLAG
                     EGDSRSLTATAFNNLGAPMEDEEIVWESSDTSVLEVDQDGNVVAVATSGTAQITARVG
                     SVRSSPVLVTVGGLAPGAVLLDDEEFTSEPEQADPSEELAVGSLFTIDVRDASSLSVG
                     DIVVSSGITPVVGRLSAVSGNTLTLEVVPLDEVFTDLDLADQTFSSSAANVKLSTEAA
                     EYFNISRTGSGAIRLEPKQELTFTGTGDGVSPQQSGTRPFGPFECVLLSGELPVLSMS
                     MGPFEYKENLTFESAWNDEEKKLIVHLQPELKVDLRIRATRADFGTERCRATLFESLS
                     PFGGALGFYLGAAVPVGIGFEIGAVSPVANVGLEIDGTVSPKISAGFSCQGGDCESVA
                     SFEDGGNVIVRPLTPSLPDGIHIRHQISVFAFAAIEPGILKQEQNGAFRRTGLTGRLL
                     IAEAGRELYTLFANRTTQALDSLDLTRAYYSAVFDSVLRAGSAERRVLDLLGLAYFPV
                     ELEIESTALDQSPETEISLGDIVGFDKGDRIPLTISTSRETLKFLGSDNIARYEAFIL
                     TDDD"
     gene            1902..2540
                     /locus_tag="PKN52_04325"
     CDS             1902..2540
                     /locus_tag="PKN52_04325"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNQ99200.1"
                     /translation="MSSCCRAGPCQEMFGVADARRDMDAYLDHGLGKVERQMLEALSL
                     PLGGARVLEIGGGVGAIQTELLLRGAASGEVVELVEAYASFAARLAQETGLADRTRFR
                     VHDLLSDPDAVEPADIVVLNRVVCCSADGLLLAAAAAGLTRRALLLSFPRSNAFTRGL
                     AWAQRALWRLFGRRYRAFAWPQEAIAAAARSGGLEVRTGGGGLVWRYLLLTR"
BASE COUNT          401 a          825 c          942 g          470 t
ORIGIN      
        1 cgtcgtcgtc ggtgaggatg aaggcctcgt agcgggcgat gttgtcgctg ccgaggaact
       61 tgagcgtctc ccggctggtg ctgatggtga gggggatccg gtcgcctttg tcgaagccta
      121 cgatgtcgcc gagggagatc tcggtctcag gtgattggtc cagggcggtg ctctcgatct
      181 ccaattccac tgggaagtag gcgagcccga gcaggtcgag cactcttctc tcggcgctgc
      241 cggcgcgcag gacggaatca aagacggcgg agtaataggc tcgcgtcaga tcgagactgt
      301 cgagcgcctg ggtggtgcgg ttggcgaaaa gggtgtacag ctcgcggccg gcctcggcga
      361 tgagcagcct gcccgtcaaa ccggtgcggc ggaaggcgcc gttctgctcc tgcttgagga
      421 ttccgggctc gatggcggcg aaggcgaaga ctgagatctg gtgacggatg tggatgccgt
      481 caggaaggct cggtgtaagg gggcggacga ttacgttgcc gccgtcctcg aaacttgcga
      541 cgctctcgca gtcaccgccc tggcaggaga agccggcgct gatcttcggg ctgaccgtgc
      601 cgtcgatctc taggccgacg ttcgctaccg gggagacggc tccgatctca aagccgatac
      661 ctaccggcac cgcggcgccc aggtagaagc cgagagcgcc gccgaacggg ctaagtgact
      721 cgaacagcgt cgccctgcac ctctccgtcc caaagtcggc tctggtcgct ctgatcctca
      781 agtcgacctt gagctccggc tgcaggtgaa cgatgagttt cttctcctca tcgttccatg
      841 cgctctcgaa cgtcaggttc tccttgtact cgaagggtcc catgctcatg ctgagaactg
      901 ggagctcgcc ggagaggagg acgcactcga acggtccgaa cgggcgggtg ccggactgct
      961 gtgggctaac gccgtctccg gtgccggtga aggtgagttc ctgcttgggc tccaagcgga
     1021 tcgcgccgga acccgttcga gatatgttga agtactcggc cgcctcagtg ctcagcttga
     1081 cgttggcggc tgaggagctg aaggtctggt cggccaggtc gaggtcggtg aacacctcgt
     1141 ctaaaggcac gacttcgagt gtcagcgtgt tcccggagac ggcgctcagg cgtcccacca
     1201 ccggggtgat accgctggag acgacgatgt caccgacact cagggagctg gcatcccgga
     1261 cgtcgatggt gaacaggctg cccaccgcga gctcttcgct cgggtcggcc tgctcaggct
     1321 cgctggtgaa ctcctcgtcg tcaagcagga cggcgcctgg ggccaggccg ccgacggtca
     1381 ccagtacggg gctggacctc acgctgccta ccctggcggt gatctgcgcc gtgccgctgg
     1441 tggcgacggc cacgacgttg ccgtcctgat cgacctcgag taccgacgtg tcgctgctct
     1501 cccagacgat ctcctcgtcc tccatcggtg cgccgaggtt gttgaaggcg gtggcggtga
     1561 ggcttcgtga atcgccttcg ccggccagga ggaaccctcc gccgctgatc tcgacccatc
     1621 ccaacgcggg tggtggcggg gttggggttc cgccgcaggc ggcgaggatg agagcaacga
     1681 gcgatagacc gagaagggcg cgaagccgca tgcacaacct ccatgatgag ccgcagtctg
     1741 ccggtcgatc cgtggacctg accacgacag ctcggctcgt tactcgccat taggctgcat
     1801 gaggcgtcgc acaggcgtca cacgcggcgc gggggctgag ctcgaggcag cgaccggccg
     1861 acgcttggcc tgtccatgcc tgcttcgcct aggatgggcc catgagttcc tgctgccgcg
     1921 ccggcccatg ccaggagatg ttcggggtgg cggacgcccg ccgcgacatg gacgcctacc
     1981 tcgaccatgg cctcggcaag gtggagcggc agatgctgga ggcgctgtcg ctccctctgg
     2041 gcggcgcccg cgtcctggag atcggcggcg gggtcggggc catccagacc gagctgctgc
     2101 tgcggggcgc ggccagcggc gaggtggtcg agctggtcga ggcctacgct tcgttcgcgg
     2161 cgcggttggc gcaggagacg ggcctggcgg accgcacgcg cttccgcgtc cacgacctgc
     2221 tgtctgatcc ggacgcggtg gagccggccg acatcgtggt gctcaacagg gtggtctgct
     2281 gctccgccga tggcctgctg ctggcggccg cggcagcggg cttgacccgg cgtgccctgc
     2341 tcttgagctt ccccaggtcc aacgccttca cgagaggcct ggcctgggcg cagcgcgcgt
     2401 tgtggcggct gttcggccgc cgctaccgcg ccttcgcctg gccgcaggag gccatcgcgg
     2461 cggcggcacg gagcggcggg ctggaggtca ggaccggtgg cggcgggctg gtctggcgct
     2521 acctgctgtt gacccgctag gcgttggcag ccatccgccg cggccgatag gtcccgccgc
     2581 tggtgtcggc aacataggat catgggaatg gataggaact cagtcgttct ccggcaca
//
LOCUS       DAOQYE010000203         2632 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_42771_length_2632_cov_3.320528, whole genome shotgun sequence.
ACCESSION   DAOQYE010000203 DAOQYE010000000
VERSION     DAOQYE010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2632
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42771_length_2632_cov_3.320528"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="PKN52_04330"
     CDS             complement(<1..286)
                     /locus_tag="PKN52_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99201.1"
                     /translation="MPQAPSECRPTYGQRLLELGAGRFDFCGIHFPQILAPFLYNPDP
                     SAFEFGQELSRDIALLAGVIGASAIVVHGPWAKMDEGAHLEATLANMRLMC"
     gene            complement(479..1726)
                     /locus_tag="PKN52_04335"
     CDS             complement(479..1726)
                     /locus_tag="PKN52_04335"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNQ99202.1"
                     /translation="MKRQPLRNLVTLAVSAVLFVASQASAQGTSIDFWYAISGKAGET
                     LQSMLQEFNSTNEFGITVNGTFSGSYGDTAQKVLAALAANSLPAGGLVPAGPLWTCRE
                     GNYLLEEYMAGPEGLPEDHFFPVLLDYNRYGGHLCSLPFNNSTMVMFYNKDLMAQAGL
                     DPSAPPQTWDELLDQAKTIAKAVPGSIGVEVRDEGWWLKGLILQNGGAIVTDDASGPA
                     FQEPAGVQALEFWKTLVDQGLMPPAQHDVSRDLFVAGRVAFLMASTATLGTVQDGAAF
                     AYGTDLLPGKAERGSTVGGAALVMFPSTPEKELATWRLLKWLTSAENSARFTEATGYV
                     PTGPDAASTDIVRSLIQEEPAYAAGFDQLAVSSQYPHFFEMGTMDNLLADAIEFVELG
                     VKTPQQALDDAANGLIKEISSNQ"
     gene            complement(1855..>2632)
                     /locus_tag="PKN52_04340"
     CDS             complement(1855..>2632)
                     /locus_tag="PKN52_04340"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNQ99203.1"
                     /translation="VMSAATAFPFVWMVGTALKSPQEILAYPPTLWPHEPRWENFVAV
                     FDRVPFLRFYLNSIVVTCLITLWQVVTSALAGYAFARLNFPFKRALFTAYIATLIIPG
                     EVTMLPLFLLVSRLGWIDTYQGLTVPFLASAFAVFFLRQFFLTIPHELEEAARLDGAG
                     RWRVLLQIVLPLARPALATITLFIFLSEWDNYLWPLIVTNSETMRTLPIGLRYFVEES
                     GSQLNLMMAGAVMAVMPILLLFFMAQRQFIEGIAMTGSKG"
BASE COUNT          446 a          801 c          935 g          450 t
ORIGIN      
        1 cacacatgag gcgcatgttc gcgagggtcg cctctaggtg cgcgccctcg tccatcttgg
       61 cccatggacc gtgtacaacg atggcactcg cgcctatgac gcccgccagc agcgcgatgt
      121 cgcgcgataa ctcctggccg aactcgaacg cggaagggtc ggggttgtag aggaacggcg
      181 ccaggatctg cgggaagtgg atcccgcaga aatcgaaacg acccgcgcca agctcgagaa
      241 gccgttgccc gtaggtcgga cggcattcgc ttggcgcttg cggcatgagt tcgacaccgc
      301 gccacggttg ccgcgcgagg atggacaacg tttcttcgat cggcttgggg aagaaggccg
      361 aggtgctgac accccgggtc ggtaaggtag ccaccgctca agttcctttc gctagatggt
      421 ggagcccctt agttgggcgg gccgcttgtg cccgcccaac taaggcggtc tcgtggcgct
      481 actggttcga ggagatctcc ttgatcaggc cgttggccgc gtcatcgagc gcctgttggg
      541 gcgtcttgac cccaagctcg acgaactcga tggcgtccgc cagcaggttg tccatggtgc
      601 ccatctcgaa gaagtgcggg tactggctgg agacggcgag ctggtcgaag ccggccgcgt
      661 acgcgggctc ctcctggatg agcgaccgga cgatgtcggt ggaggccgcg tcgggcccgg
      721 tgggcacgta cccggtcgcc tccgtgaagc gggcgctgtt ctcggccgag gtcagccact
      781 tcaggaggcg ccaggtggcg agctccttct cgggtgtgga ggggaacatg accagcgccg
      841 ccccgcctac ggtggagccg cgttcggcct tgccgggcag gaggtcagtg ccgtacgcga
      901 acgctgcgcc gtcctggacg gtgccgaggg tcgcggtgct ggccatcagg aaggcgacgc
      961 gtccggccac gaacaggtcc cttgacacgt cgtgctgcgc gggcggcatg agcccctggt
     1021 cgacgagcgt cttccagaac tcgagggcct gcacgccggc cggctcctgg aacgccgggc
     1081 cgctcgcgtc atcggtgacg atggcgccac cgttctggag tatgaggccc ttgagccacc
     1141 agccttcgtc gcgcacctcg acgccgatgc tgcctggcac ggccttggcg atcgtcttgg
     1201 cctggtcgag cagctcgtcc caggtctggg gtggtgcgct cgggtcgagg cccgcctggg
     1261 ccatcaggtc cttgttgtag aacatgacca tcgtgctgtt gttgaagggc agcgagcaga
     1321 ggtgcccgcc gtagcggttg tagtcgagca gcaccgggaa gaagtggtcc tccgggaggc
     1381 cctcgggacc ggccatgtac tcctcgagca ggtagttgcc ctcgcggcag gtccagagcg
     1441 gcccagccgg gacgaggccg cccgccggga gcgagttggc cgccagggcg gcgagcacct
     1501 tctgcgccgt gtcgccgtac gagccgctga acgtgccgtt cacggtgatg ccgaactcgt
     1561 ttgtcgagtt gaactcctgg agcatggact ggagcgtctc gcctgccttg cccgagatcg
     1621 cgtaccagaa gtcgatcgag gttccttggg cgctcgcttg agacgcgacg aatagtacgg
     1681 cgctcacggc gagcgtgacg agattgcgca acggttggcg cttcatcctt ctgttacctc
     1741 ctgtggctga ctgcggatgc ggttacaagg tccgataggg ggtgacgccg gttcctctgt
     1801 gttcccacct ccgttcggtc accggggaag cgtcgaagga cccggtcatg actgtcaccc
     1861 tttgctgccc gtcatggcga tgccttcgat gaactgcctc tgggccatga agaagagcag
     1921 aaggatgggc atgacggcca tgacggcgcc cgccatcatg aggttgagtt gactgccgct
     1981 ctcctcgacg aagtaacgca gcccgatggg cagggtccgc atggtctcgg agttcgtgac
     2041 gatcagcggc cacaggtagt tgtcccactc ggacaggaag atgaagagcg tgatcgtggc
     2101 cagggccggt cgggccaacg gcaggacgat ctgcagaagg acgcgccagc ggcctgcccc
     2161 gtcgaggcgc gccgcctcct cgagctcgtg agggatcgtc aggaagaact gccggaggaa
     2221 gaacacggcg aaggcgctgg cgaggaacgg caccgtgagt ccctggtacg tgtcgatcca
     2281 cccgagacgg ctcacgagga ggaacagggg cagcatcgtc acttcgcccg ggatgatcag
     2341 ggtggcgatg taggccgtga agagcgcccg cttgaacggg aagttcaggc gcgcgaaggc
     2401 gtagccggcg agcgcggacg tgacgacctg ccagagcgtg atcaggcacg tgacgacgat
     2461 gctgttgagg tagaagcgca ggaacggcac cctgtcgaac acggcaacga agttctccca
     2521 ccgcggctcg tggggccata gcgtcggcgg gtacgcgagg atctcctgcg gggacttcag
     2581 ggccgtgccg accatccaca cgaacgggaa ggcggtggcc gcgctcatga cg
//
LOCUS       DAOQYE010000204         2629 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_42859_length_2629_cov_2.709790, whole genome shotgun sequence.
ACCESSION   DAOQYE010000204 DAOQYE010000000
VERSION     DAOQYE010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42859_length_2629_cov_2.709790"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..524
                     /locus_tag="PKN52_04345"
     CDS             <1..524
                     /locus_tag="PKN52_04345"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HNQ99204.1"
                     /translation="TPFAAHAIDDLKAARSDVSRFNRLANLTGEPAVALPCGFTAGGL
                     PIGMQLMGRAWSEGLLLRVAGAYETAMAWRERGPDERALAEWHDDGRAEEYLRQLEGP
                     AADFGPLEAGGQGEGEAVDWVRRRAAAAGLALADTDVDGLSAQARGLIAALAGMRSSV
                     DPTGTTSALEYLS"
     gene            complement(613..2142)
                     /locus_tag="PKN52_04350"
     CDS             complement(613..2142)
                     /locus_tag="PKN52_04350"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNQ99205.1"
                     /translation="MRRALVALVIALVGIASAQDARPDLRIGVTGLPDGLDPAVNINI
                     GANGFRTLYSVFDRLIHRDFLEGGLQPGLATAWHWVDDTTLELELRQGVKFHDGSDFG
                     SEDVVYSFERIIDPASDFVTARGTLKNIANIEAIDAHTVRIHTLSPDPVLEQVLTMQE
                     TAILPAGAYATPEEASRFNQLPVGTGPYKVASFSTGDQLTLDAFDDYWGGLPTAKHVV
                     FRVIPETAARITALVNGEVDIITAVPPDQLDTIRRFSGVSVREAVLDNIHLLRYNTNN
                     PALSDKLLRQAMNLAIDRQLLSDALWGGAAEVPRSHQFKAFGDMYDADRPVDVYDPQR
                     ARELLAESDYTGQTIYLNAHPTWYVNFMAASEAIVEMWRAIGIDAQVRVVAGPAELFG
                     LAADDPMAMVNTWSNSMRYADPAGGLWANWSPSGPPQASGYWTAPEEFNRLGEEALRT
                     TDRDARRAAYWRMLDIWEDEAPGTTLYYSVEFYGIRDGVSWQPYSDFYMDLRPSNLSF
                     E"
     gene            2385..>2629
                     /locus_tag="PKN52_04355"
     CDS             2385..>2629
                     /locus_tag="PKN52_04355"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNQ99206.1"
                     /translation="MQAEVRGRRRVLIIGADGLRPDLVDPRRMPTYHALLAQGSLFTA
                     FHSAFPTHTRVNMTTLTTGTKPGRHGVINNTMYVAGAA"
BASE COUNT          402 a          845 c          943 g          439 t
ORIGIN      
        1 ccacgccgtt cgccgcccat gcgatcgacg acctgaaggc cgccaggagc gacgtgtcgc
       61 gcttcaaccg cctggccaac ctcacgggtg agccggcggt ggcgctcccg tgcggtttca
      121 cggcgggcgg cctgcccatc ggcatgcagc tcatgggacg cgcttggagc gaggggctgc
      181 tccttcgcgt cgcaggggcc tacgagacgg ccatggcctg gcgtgagcgg ggtccggacg
      241 agcgcgccct ggcggagtgg cacgacgacg ggagggccga ggagtacctc cgccaactgg
      301 aaggtccggc cgcggacttc ggcccgctag aggctggcgg ccagggcgag ggcgaggcgg
      361 tcgactgggt gcggcggcga gcggccgcag cggggctcgc gctcgcggac accgacgtgg
      421 acggcctgag cgcgcaggcg cgcggcctca tcgcggcgct ggccggcatg cgctcgtccg
      481 tcgaccccac cggaacgacc tccgcccttg agtacctgtc ctaggcatgc gcacgcgaga
      541 ccaacagggc ggcgatggct ccagagccac cgccgcccgc cttcctgtct tgctttgcct
      601 aggccgtgag ggtcactcga aggacaggtt cgagggcctc aggtccatgt agaagtcgct
      661 gtacggctgc cagctcacgc cgtcgcggat gccgtagaac tccaccgagt agtaaagggt
      721 cgtacccggc gcttcgtcct cccagatatc gagcatgcgc cagtaggcgg cccgccgggc
      781 gtcgcggtcg gtcgtgcgga gcgcttcctc gccgaggcgg ttgaactctt cgggagccgt
      841 ccagtacccg ctcgcctgcg gtggccccga cgggctccag ttcgcccaca ggccgccggc
      901 cgggtcggcg tagcgcatgg agttggacca ggtgttgacc atggccatgg ggtcgtcggc
      961 cgccagtccg aagagctcgg cgggacccgc gacgacgcgt acctgcgcgt cgatgccgat
     1021 ggcgcgccac atctcgacga tggcctcgga ggccgccatg aagttcacgt accaggtcgg
     1081 gtgggcgttc aggtagatcg tctggcccgt gtagtcggac tcggcgagca gctcgcgggc
     1141 gcgctgcggg tcgtagacgt cgaccggccg atcggcgtcg tacatgtcgc cgaaggcctt
     1201 gaactggtgg ctgcgcggta cctctgcggc gccgccccag agggcgtctg acagaagctg
     1261 gcggtcgatg gcgaggttca tggcttgccg gaggagcttg tctgacagcg ccgggttgtt
     1321 cgtgttgtag cgcaggaggt ggatgttgtc gaggaccgcc tcgcggaccg agacgccgga
     1381 gaaccgacgg atggtgtcga gctggtcggg gggaacggcc gtgatgatgt cgacctcgcc
     1441 gttgacgagc gccgtgatgc gcgccgccgt ctcggggatg accctgaaca cgacgtgctt
     1501 ggccgtcggc aggccacccc agtagtcgtc gaacgcgtcg agggtgagct ggtcgccggt
     1561 ggagaatgac gccaccttgt agggccccgt tccgacgggc agctggttga accgggaggc
     1621 ttcctcgggg gtggcgtagg ccccggctgg caggatggcc gtctcctgca tcgtcaggac
     1681 ctgctcgagc acgggatcgg gggagagggt gtggatccgc acggtgtgag cgtctatggc
     1741 ttcgatgttc gcgatgttct taagggtgcc gcgcgccgtc acgaagtcgg aggccggatc
     1801 gatgatccgt tcgaacgagt agacgacgtc ctcgctgccg aagtcgctgc cgtcgtggaa
     1861 cttgacgccc tgacggagct cgagctcgag cgtcgtgtcg tcgacccagt gccaggcggt
     1921 ggcgaggccg ggctggaggc cgccttcgag gaagtcgcgg tggatgaggc ggtcgaagac
     1981 ggagtacagg gttcggaagc cgttggcgcc gatgttgatg tttaccgcgg ggtccagtcc
     2041 gtcgggcagc cccgtgaccc cgatcctgag gtcgggcctc gcgtcctggg ccgaggcgat
     2101 gccgaccaag gctatgacga gtgcgacgag cgctctacgc attgtctagt ctccttacta
     2161 cgcggtggtg cgatgcttga aagctcctaa gctcttctct cgacgacacg gtcattgggt
     2221 caactcggtc acctcacaag ctgcacggcg gccagcccgt cctgcctgcc atgggtcttg
     2281 gccccacccc tgcctctcgc tgaactacgc gaacaatgta tcatggcagg gcaataagtt
     2341 ccatgagccg gaccatggcg ccggccgagt gttagaaggt gcgtatgcaa gctgaagtcc
     2401 gcggacgccg gcgggtcctc atcatcggag cggacggcct taggcccgac ctggtcgacc
     2461 cccggcgcat gcccacctac cacgccctcc tcgcccaggg ttcgctcttc acggcgttcc
     2521 actccgcctt cccgacccat acgcgcgtga acatgaccac cctgacgacc ggcactaagc
     2581 ccggtaggca cggcgtcatc aacaacacga tgtacgtagc gggggcagc
//
LOCUS       DAOQYE010000205         2629 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_42861_length_2629_cov_2.553225, whole genome shotgun sequence.
ACCESSION   DAOQYE010000205 DAOQYE010000000
VERSION     DAOQYE010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42861_length_2629_cov_2.553225"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /locus_tag="PKN52_04360"
     CDS             <1..529
                     /locus_tag="PKN52_04360"
                     /inference="COORDINATES: protein motif:HMM:NF015084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99207.1"
                     /translation="WASLAVGVGVAEALAGAGHECLVKWPNDLYYGGAKLGGILVERV
                     KGHLLIGIGINVDNEPPPGGTGLAGLLAKATVDDLVVEGVDAALRFVEAGSDLLPDDL
                     RRRFAARDWLSGKLVTVQDAVANAADTAGRERSAPTTVTGVACGIAPDGSLLVAVRGR
                     AEPCRVWAGTIGTHL"
     gene            724..1470
                     /locus_tag="PKN52_04365"
     CDS             724..1470
                     /locus_tag="PKN52_04365"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNQ99208.1"
                     /translation="MVLAANGDAAVAQARLALPRLVVVDANLPRLRDGRHPAQVLGRL
                     REESRRAELVLVATRNDSYVTGLAAAAGAKRILLATDGTVTGFDGEPARPTPAIVEGP
                     DVWIVDDSPAIRLLARSAFQRAGWRATEFEDLHSAQHALAAGQAPAAVVLDVYLPDGN
                     GLHHAREFVDRGAAVIMLSNLAGPEQVEQAFAAGATDIVAKPVDMRSLVARVTRAVER
                     TAVDAHTSNDGPLSSTFSSLDDGATALEAI"
     gene            1470..>2629
                     /locus_tag="PKN52_04370"
     CDS             1470..>2629
                     /locus_tag="PKN52_04370"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNQ99209.1"
                     /translation="MQRTKLTAIFVLILSASTTVAVTMGALLMRFTAAGADQSAAWRS
                     VLVALAGVGGAIGLMAVVHLSLLAARQLGTERASKRSEAWLETWSSVAAGGEPPAVER
                     HELLAASEGAALLMQEVEGEEAAVLRAALVTTGVTDNDLRIAAAGGAGSTRRSALALE
                     RLAWAADSAALPLFREAAKRSDPRVAQAALLGACRVLAVQASPEFLGREVANVIVEHA
                     ASVREPYGSRAFLAAALASTGRHQPWLSARLLEREAPEVVRAAALDAVGITRTSEAEA
                     IVSNWLGATFAAGHLIEDEVLAAAMKALARLGHLDEAMEEVAAAAARHEHTGVRVQTA
                     HALVGARPEKALPVLWELLADMAFDVRLAAATALKACGPAGEALLKRIRETC"
BASE COUNT          378 a          920 c          957 g          374 t
ORIGIN      
        1 atgggcgtca ttggcggtgg gtgtcggcgt ggcagaggcg ctcgccggcg ccggccacga
       61 gtgcctcgtg aaatggccga acgacctcta ctacgggggc gcgaagctcg gcggcatcct
      121 tgtcgagcga gtcaagggcc acctgctgat cggaatcggc atcaacgtcg acaacgaacc
      181 gccccccggc gggacaggac tggccggact gctggcgaag gcgacggtag acgacctcgt
      241 cgtcgagggt gtcgacgccg cgctacggtt cgtcgaggcg gggagtgacc tgctccccga
      301 cgacctgcgc cgccgcttcg cggcccgaga ctggctctcg ggcaagctgg tcaccgtcca
      361 ggacgccgtg gcgaacgccg ccgacaccgc ggggcgcgag cgttccgcgc ccacgaccgt
      421 cacgggcgtg gcgtgcggca tcgcccctga cggcagcctg ctcgtggcgg tcaggggccg
      481 cgcggagccg tgccgggtct gggccgggac cataggtacg cacctatgaa tggcgtccag
      541 ggcgccatcc tcgcgaacag cgagtgagtt tctccccaca ggacggcctt tgccacagct
      601 atcctgcctc tcatgcatcg gaacccttgg gcggccgatg ggggcgcaac cgcgctagta
      661 gtgcttcaca gggggcggga tcagcaagtc atatgtggcg ccatcagcgc cgcaggatgg
      721 acggtggtgt tggccgccaa cggcgacgcc gcggtggcgc aggcgcgact cgcgctgcca
      781 cggctggtgg tggtggacgc caacctgcca cggttgcgcg acggacgcca ccccgcccaa
      841 gtcctggggc ggttgcgcga ggagagccgc agggccgagc tcgtgctcgt cgccacccgc
      901 aacgactcat acgtgacggg cctggccgct gcggcaggag ccaagcgcat cctcctggcc
      961 acggacggaa cggtgaccgg cttcgacggg gagcccgcgc ggccgacgcc cgccatcgtc
     1021 gagggaccgg acgtctggat cgtcgacgac agcccggcca tccgcctgct cgcgcgcagc
     1081 gcgttccagc gggccggctg gagggcgacc gagttcgagg acctccactc cgcgcagcat
     1141 gcgctcgcag caggacaggc gcctgccgcc gtcgtcctcg atgtctacct gcccgacggc
     1201 aacggcctcc accacgccag ggagttcgtc gaccgcgggg cagccgtgat catgctgagc
     1261 aacctggcag ggcctgagca ggtagagcag gcgttcgccg ccggcgccac cgacatcgtc
     1321 gcgaaaccgg tcgacatgcg cagcctcgtc gctcgcgtga cgagagcggt cgagcgcacg
     1381 gccgtcgacg cgcacacctc caacgacggc ccactgagta gtacgttctc gtcactcgac
     1441 gacggggcga cggcgctcga agcgatctga tgcagcggac gaagctgacc gcgatcttcg
     1501 tcctcatcct gtccgcctcc accaccgtcg ccgtgacgat gggtgcgctc ctgatgaggt
     1561 tcacggcggc cggcgccgac cagtcggccg cttggcgctc cgtcctggtg gctctggccg
     1621 gtgtcggcgg ggctatcgga ctgatggccg tcgtccacct atcgctgctc gccgccaggc
     1681 aactcggcac cgagcgggcg tccaagcgct cggaggcgtg gcttgagacg tggagttctg
     1741 tcgcggccgg cggcgagccg cccgccgtag agagacatga gctactcgcg gcttccgagg
     1801 gggccgcgct gctcatgcag gaagtggagg gggaggaggc cgcggtgctg cgcgccgccc
     1861 tcgtcacgac cggcgtgacg gacaacgatc tgcgcatagc cgcggcaggc ggcgccggtt
     1921 ccaccaggcg gagcgcgctc gccctcgagc gactggcctg ggctgccgac tccgccgcgc
     1981 tgccgctctt ccgtgaggcg gcgaagcggt ccgacccgcg cgtggcccag gcggccctgc
     2041 ttggcgcgtg tcgggtcctc gccgtgcagg cgagccccga gttcctcgga cgggaggtcg
     2101 cgaacgtcat cgtcgaacat gccgcgtccg tgcgcgagcc ctacggttcg cgcgcgttcc
     2161 tcgcggcggc gctggcctcg accggccgcc atcagccttg gttgtctgcc cgcctgctcg
     2221 agcgcgaggc gcctgaggtc gtgagggcgg cagcgctgga cgccgttggc atcacgcgca
     2281 cgagcgaggc cgaggcgatc gtgtccaact ggcttggcgc caccttcgcg gccggacatc
     2341 tcatcgagga cgaggtcctg gccgccgcca tgaaggcgct cgcgcgcctc gggcatttgg
     2401 acgaggccat ggaagaggtg gccgcggcgg ccgccaggca cgagcacacc ggcgtgcgcg
     2461 tgcaaaccgc gcacgccttg gtcggtgccc gtcccgaaaa ggcccttccc gtcctatggg
     2521 agctactcgc cgacatggcg ttcgatgtgc ggctggccgc ggcgaccgcg ctcaaggcct
     2581 gcggcccggc gggggaggcg ctgctgaagc gcatccgcga gacatgcag
//
LOCUS       DAOQYE010000206         2622 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_43021_length_2622_cov_2.866771, whole genome shotgun sequence.
ACCESSION   DAOQYE010000206 DAOQYE010000000
VERSION     DAOQYE010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43021_length_2622_cov_2.866771"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..897)
                     /locus_tag="PKN52_04375"
     CDS             complement(<1..897)
                     /locus_tag="PKN52_04375"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNQ99210.1"
                     /translation="MIRPPRRLELWHVVSIGILAVYGLFLVYPLLGMLKDSVLTADGA
                     FSLRYFQRFFGERYYLNTVLNSFKLSFATMCFALLIGIPLAYLYNFYVIRGRAVLQVL
                     IVLSSMSAPFVGAYAWILLLGRSGLITRFLRNSIGIEIPTIYGFNGVVLVLTLQLYPL
                     VFLYVTGALRNVDRSLLEASQSLGTSNLKRFFRIVLPLCMTSILASALLVFMRAMADF
                     GTPLVIGEGYRTFPVEIYKQYVGETSQNYSFAAAISVVAIAITAIVFLLQKLASSRFS
                     FKMNAMNRIEPKRAKPLASVLIH"
     gene            complement(894..1991)
                     /locus_tag="PKN52_04380"
     CDS             complement(894..1991)
                     /locus_tag="PKN52_04380"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ99211.1"
                     /translation="MSVSITIKNAVKRYGDAVIIPDLSLEIRKGEFFTLLGPSGCGKT
                     TLLRMIAGFNSIEGGDFYFGTTRINDLDPAKRNIGMVFQNYAIFPHMTVRSNIAFGLR
                     NRKLPAGEIAARADEFLGLVRIAEYADRLPDRLSGGQQQRVALARALAIHPDVLLMDE
                     PLSNLDAKLRLEMREAIKEIQRSVGITTVYVTHDQEEAMAVSDRIAVMNGGVIRQIGT
                     PRALYQRPADLFVATFIGHTNALDGELYRNGAESGVSLAGVRVPLRTLVSDAGPQRVK
                     VSVRPEDFYLAGPEAGVVDGVVERSVFLGLNTHYHVRLPSGEVVEIVQESVMEDPIAP
                     ATPVRLGIKSQKINVFDEAGVVSLMAPAVGA"
     gene            complement(2120..>2622)
                     /locus_tag="PKN52_04385"
     CDS             complement(2120..>2622)
                     /locus_tag="PKN52_04385"
                     /inference="COORDINATES: protein motif:HMM:NF024739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNQ99212.1"
                     /translation="ATILVTMGGYESDAAWDYVDQLLANGVVIGSSSSIPFRSVYEGE
                     YVVGLTYEAPAVSYYEAGFDDTVKIVYMEEGTSAFPFASAIIKGARNLDLAKLFMDYV
                     VSDVAQTLWATSTARQANTTIPTTNPFLTDAADIVIVDSDHQYLSDHREEILARFQAL
                     YVKHNN"
BASE COUNT          474 a          763 c          946 g          439 t
ORIGIN      
        1 gtggatcaac accgacgcga ggggcttggc ccgcttcggc tcgatgcggt tcatggcgtt
       61 catcttgaag ctgaagcggc tggaggcgag cttctgtagg aggaagacga tggcggttat
      121 ggcgatggcc acgacgctga tggcggcggc gaagctgtag ttctgggacg tctcgccgac
      181 gtactgcttg tagatctcca ccgggaaggt ccggtagccc tcgccgatga cgagcggggt
      241 cccgaagtcg gccatggcgc gcatgaagac gagcagcgcc gacgcgagga tcgaggtcat
      301 gcagaggggc aacacgatgc gaaagaagcg cttcaggttg ctcgtgccga ggctctgcga
      361 ggcctccagc agtgaccggt ccacgttgcg cagcgccccg gtgacgtaga ggaacaccag
      421 gggatagagc tgcaacgtga ggacgaggac gacgccgttg aagccgtaga tcgtcgggat
      481 ctcgatgccg atggagttgc gcaggaaccg cgtgatgagg ccggaccgcc cgagcaggag
      541 gatccaggcg taggcgccga cgaacggggc ggacatggag ctcaggacta tcaagacttg
      601 gaggacggct cggcccctga tgacgtagaa gttgtagaga taggcgagcg ggatgccgat
      661 gagcagggcg aagcacatcg tggcgaaaga cagcttgaag ctgttcagca cggtgttcag
      721 gtagtagcgc tcgccgaaga agcgttggaa gtagcgcagc gagaaggcgc cgtcggcggt
      781 gaggacggag tccttcagca tgccgagcag ggggtagacg aggaacaggc cgtacaccgc
      841 caggataccg atgctgacga cgtgccacag ttccagccgt ctgggagggc ggatcatgct
      901 cctaccgccg gggccatcaa cgagacgacg ccggcctcgt cgaagacgtt gatcttctga
      961 gacttgatgc cgaggcgcac gggtgtggcg ggcgcgatcg ggtcctccat gacggactcc
     1021 tggacgatct cgacgacctc gcccgaggga agacgcacgt ggtagtgcgt gttcagcccc
     1081 aggaacacgc tgcgctccac caccccgtcg acgacgcccg cctcggggcc ggcgaggtag
     1141 aagtcctcgg ggcgcaccga gaccttgacg cgttgcgggc ccgcgtcgct cacaagggtc
     1201 cgcagcggaa cgcggacgcc ggcgagcgac acgccgctct cggcgccgtt cctgtagagc
     1261 tcgccgtcga gcgcgttcgt atgaccgatg aaggtggcca cgaagaggtc ggccggtcgc
     1321 tgatagagcg cccgcggggt gccgatctgc ctgatcaccc cgccgttcat cacggcgatg
     1381 cgatcggaga ccgccatggc ctcctcctgg tcgtgggtga cgtagacggt ggtgatgccg
     1441 acgctccgct ggatctcctt gatcgcctcg cgcatctcca gacggagctt ggcgtcgagg
     1501 ttggagagcg gctcgtccat gaggagcacg tctggatgga tggccaaggc tctcgccaag
     1561 gccacgcgtt gctgttgacc gcccgagagg cggtctggca aacggtccgc gtactcggcg
     1621 atgcgcacga ggccgaggaa ctcgtccgcc cgggcggcga tctcgccggc cggcaacttg
     1681 cggttcctca ggccgaacgc gatgttggag cgcacggtca tgtgagggaa gatggcgtag
     1741 ttctggaaca ccatcccgat gttccgtttc gcggggtcga ggtcgttgat gcgggtggtg
     1801 ccgaagtaga agtccccacc ctcgatggag ttgaacccgg cgatcatccg aagcagggtc
     1861 gtcttgccgc aaccgctcgg accgagcagg gtgaagaact cgcccttgcg gatctcgagc
     1921 gagaggtccg ggatgatcac cgcgtcgcca tagcgtttca cggcgttctt gatggtgatg
     1981 ctgacgctca tgcgcgcggt ccttcccctg accggtagga gccgcgtcgg gcggggctcc
     2041 taccggtgtc gaacgtgagc gcctttccga aggtaagtac gccgcggccg cgctcgcgcc
     2101 gggcgaagcc taggctccct cagttgttgt gcttgacgta gagcgcctgg aaccgcgcca
     2161 ggatctcctc ccggtggtcg ctcaggtact ggtggtcgga gtcgacgatg acgatgtccg
     2221 cggcgtcggt gaggaacggg ttggtagtcg ggatggtggt gttggcctga cgcgccgtcg
     2281 aggtggccca caacgtctgc gccacgtcgg agacgacgta atccatgaac agcttggcga
     2341 ggtcgaggtt acgcgcgccc ttgatgatgg cggaggcgaa ggggaaggcc gaggtgccct
     2401 cctccatgta cacgatcttg acggtgtcgt cgaaaccggc ctcgtagtag gacacggccg
     2461 gggcctcgta cgtgaggccg accacgtact cgccctcgta gacggagcgg aacgggatgc
     2521 tcgagctgga cccgatgacc acgccgttgg cgaggagctg gtcgacgtag tcccaggcgg
     2581 cgtcgctctc gtagccaccc atggtcacga ggatcgtggc ga
//
LOCUS       DAOQYE010000207         2620 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_43073_length_2620_cov_3.276413, whole genome shotgun sequence.
ACCESSION   DAOQYE010000207 DAOQYE010000000
VERSION     DAOQYE010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43073_length_2620_cov_3.276413"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1683
                     /locus_tag="PKN52_04390"
     CDS             <1..1683
                     /locus_tag="PKN52_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99213.1"
                     /translation="EPGAGTVQSGVSVIHNPVGSGVAAQNTFRRPAALLAYQVGYQDE
                     GGTDQTLTPPVLAGTVDVPSTQALGVFQALTDIVMGESPWSPVVSEGLPLPLYEGRRT
                     YYELVLLGPALDVVTPPPLYSDPRFPGQRSHWNDVIGGKALELFMNDIALPVLESFAF
                     GSVGHFEAGKLAKLREDFKVVNDKHLANLGIFLKEGGGYAEGVKFILDELVTNERYRL
                     DFFEVMVEAMPESERNKVSFEAVEARLKTKAAAAAVAAGVQIALSAGDIGAILKDLND
                     ALPAHGWIATAAPTLFVMSPEFAYFTKSDPQVEFQVSTRGPVSGTFLYRWSTTGRYGD
                     LTDGPKKGTTIDTTYASVWYTHRTPLNISDDQTDGVWVEVYEVEPGVTTIPTGQAPTA
                     RMAAELKGFNREIDSRIEVQYGFTPAGYYNEGRSVECATMYVRIPKQAGVKKYTLHFE
                     GWGGINHPNNYNVYLRANYSYNWYIDPSNADGWTWPFTAVCNWFRPSDGQPFGPPVSF
                     QNYDDGNDYLIAVYTYVGFVGEVPEGALMQADHVRLWYDWASKGTLTATVTR"
     gene            1751..>2620
                     /locus_tag="PKN52_04395"
     CDS             1751..>2620
                     /locus_tag="PKN52_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99214.1"
                     /translation="MLSQRPKIGPLLRQRPRLLERLPDSPGWVVWLEAPFGYGKSVLS
                     AQWAEELEAGGWRVLWVSMQDRGVRDALAELLELPAEATWSDIRSSLWSTETLLVLED
                     LGEAADVDELLPDQRGLLLLASRGSLASPALLRSLTAGAALHLTAGDLAFTRDEAAQL
                     FASKEAGETAWAESGGWPLPLHVAALTGRAAPAEPLMVGVRHSLSPELWEELLFQSAV
                     PYLPSGAATPASAGLAAAGFSQEIAAGYRLHELMALSVRRANSAGVADAVRTHATRQP
                     LAMRAEAFARAGLV"
BASE COUNT          417 a          942 c          883 g          378 t
ORIGIN      
        1 gaacccggcg ccggcaccgt gcagtccggg gtatccgtga tccacaaccc ggtcggctca
       61 ggcgtggccg cgcagaacac cttccgcagg cccgcggcgc tgctcgccta ccaggtcggc
      121 taccaggacg agggcggcac cgaccagacc ctcacgccgc ccgtgctcgc gggaacggtc
      181 gacgtgccat cgacccaagc gctcggcgtg ttccaagcgc tcaccgacat cgtcatgggc
      241 gagtcgccgt ggtcacccgt cgtcagcgag ggcctccctc tgcccctgta cgagggcagg
      301 cgcacgtact acgagctcgt gctgctcgga ccggcgctcg acgttgtcac ccctccgccc
      361 ctgtacagcg acccccggtt ccccggccag cgcagccact ggaacgacgt catcggcggc
      421 aaggcgctcg agctgttcat gaacgacatc gccctgcccg tgctcgagtc gttcgcgttc
      481 ggcagcgtcg gccacttcga ggccggcaag ctcgccaagc tgcgcgagga cttcaaggtc
      541 gtcaacgaca agcacctggc caacctcggc atcttcctga aggagggtgg cggctacgcc
      601 gagggcgtca agttcatcct cgacgagctc gtcacgaacg agcgctaccg gctcgacttc
      661 ttcgaggtca tggtcgaggc catgccggag agcgagcgga acaaggtgag cttcgaggcg
      721 gtcgaggcgc gcctgaagac caaggcagcg gccgccgccg tggccgcggg cgtgcagatc
      781 gcgctcagcg ctggcgacat cggcgccatc ctcaaggacc tgaacgacgc gcttccggcc
      841 cacggttgga tcgccaccgc cgcgcccacc ctgttcgtga tgtcgccgga gttcgcctac
      901 ttcacgaaga gcgacccgca ggtcgagttc caggtaagca cccgcggacc cgtctcgggc
      961 accttcctct accgctggtc cacgaccggc cgctacggcg acctcaccga cggcccgaag
     1021 aagggcacga ccatcgacac gacctacgcc agcgtctggt acacgcaccg cacccccctc
     1081 aacatctcgg acgaccagac ggacggcgtg tgggtggagg tgtacgaggt ggagccgggc
     1141 gtcacgacga tcccgaccgg tcaggcgccg acggcgcgca tggcggcaga gctcaaaggc
     1201 ttcaaccgcg agatcgactc gcgcatcgag gtccagtacg ggttcacgcc ggccgggtac
     1261 tacaacgagg ggaggtccgt cgagtgcgcc accatgtacg tgcgcatccc gaagcaggcc
     1321 ggcgtcaaga agtacacgct ccacttcgag ggctggggcg gcatcaacca cccgaacaac
     1381 tacaacgtct acctccgggc caactactcg tacaactggt acatcgaccc gagcaacgcc
     1441 gacggttgga cgtggccgtt caccgcggtc tgcaactggt tccgcccgtc ggacggccag
     1501 ccgttcgggc cgccggtcag tttccagaac tacgacgacg gcaacgacta cctgatcgcc
     1561 gtgtacacct acgtcggctt cgtaggcgag gtgccggagg gcgctctaat gcaagcggat
     1621 cacgtcaggc tctggtacga ctgggcctcg aagggcacgt tgacggcgac ggtcaccagg
     1681 tgaggtgacc cgcgagcatg gcggcggcac cgcgcgttgg tggcggtacc gctagactgg
     1741 cgccaccgcc atgctctccc aacgcccgaa gatcggccca ctgcttcgcc agcgaccgcg
     1801 gctcctcgag cggttgccgg acagccccgg ttgggtcgtg tggttggagg cgcccttcgg
     1861 ctacggcaag agcgtcctgt cggcccagtg ggccgaggag ctcgaggccg gcggctggcg
     1921 ggtgctgtgg gtctccatgc aggaccgggg ggtgcgcgac gcgctggcgg aactgctcga
     1981 gctgccggcc gaggcgacct ggtccgacat ccggtcgtca ctatggtcga cggagacgct
     2041 gctcgtactc gaggacctag gcgaggccgc cgacgtcgac gagctcctcc ccgaccagcg
     2101 cggcctactg ctgctggcca gccgcggcag cctggcatcg ccggcgctgc tgcgcagcct
     2161 caccgcgggg gccgccctgc acctcaccgc cggcgacctg gcgttcaccc gggacgaggc
     2221 cgcccagctg ttcgcctcca aggaggcggg agagaccgcg tgggccgaat cgggcggctg
     2281 gcccttgccg ttgcacgtcg ccgcgctaac gggcagggcg gcgccggccg agccgctcat
     2341 ggtgggggtg cgccacagcc tgtctcccga actctgggag gagctgctgt tccagtcggc
     2401 cgtcccgtac cttccgtcag gcgcggcgac accggcctcc gccggcctgg ccgcggccgg
     2461 cttcagccaa gagatcgccg ccggctaccg cctgcacgag ctcatggccc tgtcggtgcg
     2521 gcgcgccaac tccgcggggg tcgcggacgc cgtccgcacg cacgccaccc ggcagccgtt
     2581 ggccatgcgc gccgaggcgt tcgcgcgtgc aggcctggtg
//
LOCUS       DAOQYE010000208         2614 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_43214_length_2614_cov_3.785854, whole genome shotgun sequence.
ACCESSION   DAOQYE010000208 DAOQYE010000000
VERSION     DAOQYE010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43214_length_2614_cov_3.785854"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1269
                     /locus_tag="PKN52_04400"
     CDS             <1..1269
                     /locus_tag="PKN52_04400"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HNQ99215.1"
                     /translation="MPAYLERSVDKAISESLQSFGAVILQGPRAVGKTTSGLHAAASS
                     LRLDASTDLVTLAEVSPQTLLAGATPRLIDEWQLAPTIWNAVRHEVDRRGSPGQFILT
                     GSATPADDRTRHSGAGRFRRITMRPMTLAESGDSTAAFSLADLLEGKPVAGLGGPTVP
                     RYAELIERGGWPALVLHGASSARTYLTSYLEDIARVDLPASDLVVDPIRMLALVRALA
                     RNVACEISAARLGAEAEITEQAGSTKGVSAQSVRKYLDALSRIFVVEEQPAWAPHLRS
                     RVRVRVQPKWHFVDPSLAAAALGAGSESLLADLNTFGLLFESLCVRDLRVHADALGGS
                     VYHYRDDSNLEVDAIVELGDGTWSAFEVKLGGGSHIEAAAANLTKLRGKVSARRAEQL
                     RSLNVITAGNASFTRPDGINVVALGHLAAS"
     gene            complement(1360..2142)
                     /locus_tag="PKN52_04405"
     CDS             complement(1360..2142)
                     /locus_tag="PKN52_04405"
                     /inference="COORDINATES: protein motif:HMM:NF019359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNQ99216.1"
                     /translation="MPVKRAENDAMTVGEVARLANVSVRTLHHYDEFGLLSPSERSEA
                     GYRLYTPGDLERLHAVLAYRELGLELGAIQELLSDQAADPMEHLKRQEAVLNARLEKL
                     LAVRRSLRKQMEAVHMGINLNPKELLEVFGDEDPTKHAKEAEERWGDTDAYRESQRRT
                     KGYTKEDWLRISKESEAIEAGFAELLAEGVAPTDQRAMEAAEAHRRHISRCYYECGYD
                     IHRGLAEMYLADPRFTAHYEQRAEGLAAFVSAAIIANAEARG"
     gene            2249..>2614
                     /locus_tag="PKN52_04410"
     CDS             2249..>2614
                     /locus_tag="PKN52_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HNQ99217.1"
                     /translation="MTPDGTTALDLEALREDVMAIAKAAGEAILAVYATDFAVSTKAD
                     ASPVTAADVASQKVVEDGLATLTPGVPVIAEESPAPAFEERRSWRRFWLVDPLDGTKE
                     FVKRNGEFSVNIALIEDGVP"
BASE COUNT          431 a          904 c          852 g          427 t
ORIGIN      
        1 atgcccgcct acctcgagcg ctccgttgac aaagcgatct ccgagtcgct ccagtcgttc
       61 ggcgccgtga tcctgcaggg tccacgtgcc gtcggcaaga ccaccagcgg ccttcatgcg
      121 gcagccagct cgttgaggct cgatgcttca acggacttgg tcacgctagc ggaggtctcc
      181 ccgcagacgt tgctggcggg agccacacca cgcctgatcg acgaatggca actggcgccg
      241 accatctgga acgcggtcag acacgaagtg gaccgcagag gcagcccagg gcagttcatc
      301 ctcacgggct cggccacccc tgccgatgac cgcacgcgcc acagcggagc cggacgcttc
      361 cgccgcataa ccatgaggcc gatgacgctc gccgagagcg gtgacagcac ggcggcattc
      421 agcctcgcgg acctgttgga aggcaagccc gtggcaggcc ttggcggtcc cacggtccca
      481 cggtacgcgg agctcatcga gcgcggcggc tggcccgcgc tcgtgctaca cggcgccagc
      541 tcggcgcgca cctacctcac gagttacctg gaggacatcg cgcgcgtcga cctgcccgcc
      601 agcgacctcg tcgtggaccc gataaggatg ctcgccctgg taagggccct cgcacgcaac
      661 gtcgcttgcg agatctcggc cgcgcgtcta ggagcggaag cggagatcac agagcaggcc
      721 gggtcgacaa agggcgtctc ggcgcaaagc gtgcgcaagt acctggatgc cctcagccgc
      781 atattcgtcg tggaggagca gcccgcctgg gcaccgcacc tacgctcaag agtgcgcgtg
      841 agagtacagc cgaagtggca cttcgtcgac ccatcgctcg ccgccgcagc cctcggcgcc
      901 ggctcagaaa gcctcttggc agacctcaac acgttcgggc tcctgttcga gtcgttatgc
      961 gtacgcgatc tgcgcgtcca tgccgatgca ctaggtggca gcgtgtacca ctaccgggat
     1021 gactcgaacc tggaggtcga tgccatcgtc gagctggggg acggcacctg gtcagctttc
     1081 gaggtcaagc tcgggggcgg ctcgcatatc gaagccgcgg ccgccaacct cacgaagctg
     1141 cgaggcaagg tctccgctcg ccgcgccgag cagctgaggt cactcaacgt gatcacggcc
     1201 gggaacgcca gtttcacgcg gcccgacggc atcaacgtag ttgcgcttgg acacctcgcc
     1261 gcgagctgaa gcgaggccgc ggcgatgatc gccgcggcca tgcgcgggag caggtacggg
     1321 cggtacctgc tcgggttgct ggacggctgg gggccgctgc tagccccgcg cctcggcgtt
     1381 cgcgatgatc gcggccgaca cgaaagcggc cagcccttcg gcgcgctgct cgtagtgcgc
     1441 ggtgaagcgc gggtcggcca gatacatctc ggccagaccc cggtggatat cgtagccgca
     1501 ctcgtagtag cagcgcgaga tgtgccgcct atgcgcctcg gccgcctcca tagcacgctg
     1561 gtccgtcggg gcgacgccct cggccagcag ctcggcgaac ccggcctcga tggcctcgct
     1621 ctccttcgag atgcgcagcc agtcctcctt cgtgtagccc ttcgtgcggc gctggctctc
     1681 gcggtaggcg tccgtgtcgc cccagcgctc ctcggcttcc ttcgcgtgct tggtgggatc
     1741 ctcgtcgccg aagacctcca gcagctcctt cgggttcagg ttgattccca tgtgtaccgc
     1801 ctccatctgc ttgcgtagcg accgccgcac cgcgaggagc ttctcgagcc gcgcgttgag
     1861 caccgcctct tgacgcttca agtgctccat ggggtcggcc gcctgatcgg agaggagctc
     1921 ctggatggcg ccaagctcga gaccgagttc gcggtaagcg agcacggcgt gcaaccgttc
     1981 caggtcgccc ggcgtgtata ggcgatagcc ggcctcgctg cgctcgctcg gcgacagcaa
     2041 gccgaactcg tcgtaatgat ggagcgtccg gacgctcacg ttcgcgagcc tggccacctc
     2101 gccgaccgtc atggcgtcgt tctcggcacg tttcacaggc attgcctcct ttccgacgtc
     2161 caccctagag cctcacgtta cgtgagggtc aaggggggag gccaccggtt ccactacgcc
     2221 ggtgcggcag cgcgttacgc tagccgcaat gacacccgac gggacgaccg ctctggactt
     2281 agaggctcta cgtgaagacg tgatggccat cgccaaggcc gccggcgagg cgatcctggc
     2341 ggtctacgcc accgacttcg ccgtcagcac caaagccgac gcgtcaccgg tgacggccgc
     2401 cgatgtcgcc tcgcaaaagg tcgtagagga cggcctcgcc accctcacac cgggtgtgcc
     2461 ggtcatcgcc gaggagtcac cggcacccgc gttcgaggag cgccggtcgt ggcgacgctt
     2521 ctggctggtc gacccgctcg acggcaccaa ggagttcgtc aagcgcaacg gcgagttctc
     2581 cgtgaacatc gccctgatcg aggacggagt gccg
//
LOCUS       DAOQYE010000209         2613 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_43256_length_2613_cov_2.337373, whole genome shotgun sequence.
ACCESSION   DAOQYE010000209 DAOQYE010000000
VERSION     DAOQYE010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43256_length_2613_cov_2.337373"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="PKN52_04415"
     CDS             complement(<1..198)
                     /locus_tag="PKN52_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNQ99218.1"
                     /translation="MRVRVASAGTGKTTSLVRRYLELVDDGVPLGRIAGVTFTRAAAG
                     ELRQRIAAGLDEVLGSATYLGG"
     gene            complement(195..2537)
                     /locus_tag="PKN52_04420"
     CDS             complement(195..2537)
                     /locus_tag="PKN52_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99219.1"
                     /translation="MKLTVTVGPPGSGRTERMLAAARAACEAGRRVWWVGLPAQRAHV
                     LHRLTANGYAALGFEFMSAQQLYYRLLTQATTILPLVVGSARLVRVVEALVQVNRALP
                     TPGEARLFAAAIAEAKRFGVDFEAYERLAVDAEQRRFAAVYRAYQAGMGGVWDYDDAR
                     SAATALVGEERCEVGADLVIVDGLREVGPLELRFLRGLAKHVDVRLTLPALPPGLEAD
                     EELPARAAAAVERYGAPNPVAEARWVMSSLKRDLFQGGYRPLDLAVIAPPERARALTV
                     LAEEYGVPLMDESPLALADGGHGRTLVDLLELPEMPTASRLLAVPELAPLGRLALEAG
                     VAGNDAVEELARLNGLTEAWRKWQGRLEASGDPVVWARGLIEEALPGAAPTFAAAALG
                     KAQEAARLGSGPGFRAWWAALLQDAREARHEPAGVALLDATLASGRRYRKAYLLGAVE
                     GAYGGNEREDYFLPEERRREPSESFERLGLPRRFQGRGRDVAAELLQRADRLVITAPR
                     GDQGGQFVADAALFGGETPHDLPPIPAGSPVELGGAARFTADLSAVDLGEPYVEGLRR
                     YRQCSFRYWGESVLRLVRDPEPSDAARALRAALVRSGPEPLTRERIATLAVTFPAYAT
                     WLERFAERLARLTYGVTLGGRQGYAVARLDATERQELAGGGRTVTVYRFVAWGDAWDD
                     QSAEQAMRDRWTEYWAVGALFAQPSFRVSEARIVVWPIGGEPIDLAPRGVKPDWRRVV
                     DRRRLVEETLPAFARGEVRPNPGFHCRTCPVFDLCREGVR"
BASE COUNT          358 a          977 c          951 g          327 t
ORIGIN      
        1 ccccccgaga taggtcgccg atccgagcac ctcgtccaag cctgccgcga tgcgctggcg
       61 cagttcgccc gccgccgcgc gcgtgaaggt gacgcccgcg atgcgcccga gcgggacccc
      121 atcgtctacg agctccaggt agcggcggac gagcgatgtc gtcttacccg tgccggccga
      181 ggcgacgcgt accctcaccg cacgccctcc cggcacaggt cgaacacggg gcacgtccgg
      241 cagtggaagc cggggttggg gcgcacctca ccgcgcgcga aggccgggag cgtctcctcc
      301 accagccgcc gtcggtcaac tacgcgccgc cagtccggct tcacgccgcg cggcgccagg
      361 tcgatcggtt caccgccgat cggccatacg acgatgcgcg cctcggagac ccggaaggac
      421 ggctgggcga acagagcgcc gacggcccag tactccgtcc agcggtcgcg catggcctgt
      481 tcggcgctct ggtcgtccca cgcgtcgccc caggcgacga agcggtagac cgtcaccgtg
      541 cgcccgccgc cggcgagctc ctgccgctcg gtagcgtcga gtcgtgcgac cgcgtacccc
      601 tgccggccgc ccagcgtgac gccgtacgtc aggcgggcca gacgctccgc gaagcgctcg
      661 agccacgtgg cgtaggccgg gaaggtcacc gcgagggtcg cgatgcgctc ccgcgtcaac
      721 ggctccggcc cgctgcgcac gagtgcggcg cgcagcgcgc gggcggcgtc ggacggctca
      781 gggtctctca ccaggcgcag caccgactcg ccccagtacc tgaacgagca ctgccggtag
      841 cgcctgaggc cctcgacgta gggctcgccg aggtcgacgg cgctcaggtc ggcggtgaag
      901 cgggctgcgc cacccagttc caccgggctg cccgccggga tcggcgggag gtcgtgcggt
      961 gtctccccgc cgaacagcgc ggcgtcggct acgaactggc cgccctggtc gccccgcggt
     1021 gccgtgatga cgagccggtc ggcgcgctgg aggagctcgg ctgccacgtc ccgccccctt
     1081 ccctggaagc gccgcggcag gccgaggcgc tcgaacgact cgctcggctc cctgcgccgc
     1141 tcctccggga gaaagtagtc ctcgcgctcg ttgccgccgt aagccccctc aacggcgccg
     1201 aggaggtagg ccttgcggta gcgcctgccg gaggcgagcg tggcgtcgag caaggccacg
     1261 ccggccggct cgtgacgcgc ttcgcgcgcg tcttgcagga gggccgccca ccaggccctg
     1321 aagccggggc cggagccgag gcgcgccgcc tcctgggcct tgccgagcgc ggcggcggcg
     1381 aaggtcggcg ccgcgccggg cagtgcctcc tcgatgaggc cacgtgccca cacgacgggg
     1441 tcgccactcg cctcgaggcg cccctgccac ttgcgccacg cttcggtgag cccgttaagg
     1501 cgcgcgagct cctcgacggc gtcgttgccg gcgaccccgg cctcgagcgc gaggcgcccg
     1561 agcggcgcga gttccggcac ggccagcagg cgcgaggcgg tcggcatctc gggcagctca
     1621 agcagatcga cgagggtccg accgtggccg ccgtcggcca gcgccagcgg ggactcgtcc
     1681 atcaggggca ctccgtactc ttcagccagg accgtcagcg cgcgcgcgcg ttccgggggg
     1741 gcgatcaccg cgaggtcgag cggccggtag ccgccctgga agaggtcacg cttcagggag
     1801 ctcatcaccc agcgtgcttc cgcgacgggg ttcggggcgc cgtagcgctc gacggccgcg
     1861 gcggcgcgcg ccggcagctc ctcgtcggcc tccagaccgg gcgggagggc cggcagcgtg
     1921 aggcgcacgt ctacgtgctt ggcaagcccg cggaggaaac gtagctcgag cgggccgacc
     1981 tcgcgcaggc cgtcgactat gacgaggtcg gcgccgacct cgcaccgctc ctcgccgacg
     2041 agcgccgtag cggcgctgcg ggcgtcgtcg tagtcccaca cgccacccat tccggcctgg
     2101 taggcgcggt agacggcggc gaacctgcgc tgctcggcat cgacggccag gcgctcgtag
     2161 gcctcgaagt cgaccccgaa gcgcttggcc tccgcgatgg ctgcagcgaa caggcgcgcc
     2221 tcgcccgggg taggcagcgc acggttgacc tgaacgagcg cctcgacgac ccgcactagc
     2281 ctggcgctgc ccacgacgag cggcagtatc gtcgttgcct gcgtcagcag ccggtagtag
     2341 agctgctggg cactcatgaa ctcgaagccg agcgccgcgt agccgttcgc cgtcaggcgg
     2401 tgaaggacgt gggcgcgctg agcggggagc ccgacccacc agacccgcct gccggcttcg
     2461 caggccgccc tggcggcggc gagcatgcgc tccgtgcggc cggagccggg cgggccgacc
     2521 gtgacggtta gcttcacccg agcatcatac gaaccgttcg cgacgtcaga cgacgtgcgc
     2581 cggacgggcg atctcgcgat cttgcgctac ggc
//
LOCUS       DAOQYE010000210         2589 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_43884_length_2589_cov_2.865036, whole genome shotgun sequence.
ACCESSION   DAOQYE010000210 DAOQYE010000000
VERSION     DAOQYE010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2589
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43884_length_2589_cov_2.865036"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1838)
                     /locus_tag="PKN52_04425"
     CDS             complement(<1..1838)
                     /locus_tag="PKN52_04425"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001960.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HNQ99220.1"
                     /translation="MDLLITLVPLAAIVALVAAFVLARSIMAAPTGDARMVEISDAVR
                     QGAAAYMNRQYRTIAIVAVIIAAVFAVVALTSGNDHDRTLWWWTTAGFAIGALFSAIS
                     GFVGMNVAVRANVRVAQAARGGLAGALRIAFSGGAVAGLAVAGLALLGVSGFFWLFHV
                     VLDLPNPVEPLVGFAFGASLISLFARVGGGIYTKAADVGADLVGKVEAGIPEDDPRNP
                     AVIADNVGDNVGDCAGMGADLFETYAVTAIGAVFLGYLLPGTGAVTNLVLYPLLLGAI
                     AIIASIIGTQFVRLPEGSNNIMGALYKGVFVSAGISVVAFGILTYAMFNGFDFTILGR
                     NMGWGSLFLASLVGIVVTIAMMFITEYYTSTRFAPVRRVAKASETGSATNIISGLAVG
                     MQSTAFPVIVLVLAIFVAYSIAGLYGIAVAAVAMLSVTGMVVAMDTYGPITDNAGGIA
                     EMADLPANVRANTDALDAVGNTTKAVTKGYAIGSAALAALVLFADFAERLKLVDPVKF
                     GAGAFRLDDPIILVGLLIGAMLPFLFSAFLMESVGKAAGSVIEEVRRQFRTIKGIMEG
                     KAKPDYGKAVDIVTAAALREMALPGIIAVAASLVVGFLFGPLALGGL"
     gene            2219..>2589
                     /locus_tag="PKN52_04430"
     CDS             2219..>2589
                     /locus_tag="PKN52_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99221.1"
                     /translation="MDRPANPGESAAEAGGQARMEATTEPGASPLAGKRAPRALLADV
                     PRLVTDYYTVKPDPARVDERVAFGTSGHRGTSGKASFNEDHILAVCQAIADRRAAQGI
                     TGPLFLGADTHALSTPALVTA"
BASE COUNT          439 a          849 c          949 g          352 t
ORIGIN      
        1 aggccgccga gcgccagcgg gccgaagagg aagccgacca ctagggaagc cgccacggcg
       61 atgatgcctg ggagggccat ctcgcgcagg gccgcggccg tgacgatgtc gacggccttg
      121 ccgtagtccg gcttcgcctt gccttccatg atgcccttga tggtgcggaa ctgcctgcgg
      181 acctcctcga tcaccgaacc ggccgccttg ccgaccgact ccatgaggaa cgcgctgaag
      241 aggaacggca gcatggcgcc gatgaggaga cccactagga tgatcgggtc gtccaggcgg
      301 aaggcgccgg cgccgaactt gacgggatcg acgagcttga ggcgctcggc gaagtcggcg
      361 aagaggacga gggcggccag cgcagcggag ccgatggcgt agcccttcgt gacggccttg
      421 gtggtgttgc cgaccgcgtc gagcgcgtcg gtattggcgc gcacgttggc gggcaggtcg
      481 gccatctcgg cgatgccgcc tgcgttgtcc gtgatcgggc cgtacgtgtc catggcgacg
      541 accatgccgg tgacggacag catggcgacc gctgcgacgg cgatgccgta gaggcccgcg
      601 atcgagtagg cgacgaagat ggcgagcacg agcacgatga ccgggaaggc ggtcgactgc
      661 atgccgacgg cgaggcctga gatgatgttc gtggcactgc cggtctcgga cgccttggcg
      721 acgcgtctga ccggcgcgaa gcgcgtgctc gtgtagtact cggtgatgaa catcatggcg
      781 atggtcacca cgatcccgac gaggctggca aggaagagcg agccccagcc catgttccga
      841 ccgaggatgg tgaaatcgaa gccgttgaac atggcgtagg tgaggatgcc gaacgccacg
      901 acgctgatgc cggcgctcac gaacacgccc ttgtagagcg cacccatgat gttgttggag
      961 ccttccggca ggcgcacgaa ctgcgtgccg atgatgctcg cgatgatggc gatggcgccg
     1021 aggaggagcg ggtagaggac caggttcgtg accgcgccgg tgcctgggag caggtagccg
     1081 aggaagacgg cgccgatggc ggtgacggcg taggtctcga acaggtcggc gcccataccg
     1141 gcgcagtcgc cgacgttgtc gcccacgttg tccgcgatca cggccgggtt gcgggggtcg
     1201 tcctcaggga tgcccgcctc gaccttgccg acgaggtcgg cgccgacgtc ggcggccttc
     1261 gtgtagatgc cgccccccac acgcgcgaag aggctgatga gcgaggcgcc gaacgcgaag
     1321 ccgacgaggg gctcgaccgg gttggggagg tccaagacca cgtggaagag ccagaagaag
     1381 ccggagacgc cgaggagcgc gaggcccgcg accgccaggc ccgcgacggc cccgccgctg
     1441 aacgcgatgc gtagagcgcc agccagtccg ccgcgcgcgg cctgggctac acgcacgttg
     1501 gcacgcacgg cgacgttcat gccgacgaag ccgctgatgg cgctgaagag cgcgccgatc
     1561 gcgaagcccg ccgtcgtcca ccaccacagc gtgcggtcgt ggtcgttgcc ggacgtgagc
     1621 gccacgacgg cgaacacggc ggcgatgatc acggcgacga tggcgatggt ccggtactgg
     1681 cggttcatgt acgccgcggc gccttggcgc acggcgtcgg agatctcgac catgcgcgcg
     1741 tcgccggtcg gcgcggccat gatgctgcgc gccagcacga aggctgccac cagcgccacg
     1801 atggcggcca gcggaactag ggtgatcaac aggtccattc ttcctccacc agactttcta
     1861 tgcgcgcggt ccctaaagcg cgcagttccc cagctgggta cttgcgttcg cgttgccacc
     1921 gctccggagc ttgacccccg gacggcgctc tgccgctgcc gtgccgccgg gccgctgctc
     1981 gtgaagggcg gcggttcgcc cgttgagcag cggcaagctt accactagcg gccggcccgg
     2041 cattccgggg cgagcgccag cccgcaaggc cggccgagcg gttgccggcg gccagggtcg
     2101 gcgacggctc gccaggcgcg gttctgcagg cgcggttcgg cggagcgcgg tagccttggg
     2161 cgaccccgag cgccaggtgg cgcgccctgg agacctttgg acgtcgagag gtgaaggcat
     2221 ggatagaccc gccaacccag gagagagcgc ggccgaagcc ggagggcaag cgcgcatgga
     2281 agccacgacc gaacccggcg cgagcccgct cgcggggaag cgggccccaa gggcgctgct
     2341 agccgatgta ccgcgtctcg tgaccgacta ctacacggtc aagcccgacc ccgctcgggt
     2401 cgacgagcgc gtggcgttcg gcacctccgg gcaccgcggc acgagcggga aggcgagctt
     2461 caacgaagat cacatcctcg ccgtttgcca ggccatcgcg gataggcgcg cggcgcaggg
     2521 cataaccggc cccctcttcc tcggcgccga cacgcacgcc ctgtcgaccc cggcgctcgt
     2581 cacggccat
//
LOCUS       DAOQYE010000211         2588 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_43893_length_2588_cov_6.068298, whole genome shotgun sequence.
ACCESSION   DAOQYE010000211 DAOQYE010000000
VERSION     DAOQYE010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2588
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43893_length_2588_cov_6.068298"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="PKN52_04435"
     CDS             complement(<1..364)
                     /locus_tag="PKN52_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99222.1"
                     /translation="MAFTVEDGTGVAGANSLCDVAFADTYHADRANSSWASSTQAAKE
                     AALIKATDYIEQRFGGRFKGRREHETQVLSFPRLCLYTRDGVLVTGVPDRLKAAVSEY
                     ALKALTVSLFLEPEVDDRG"
     gene            complement(376..762)
                     /locus_tag="PKN52_04440"
     CDS             complement(376..762)
                     /locus_tag="PKN52_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99223.1"
                     /translation="MARPAVLYKARERRRAAADFKKFGPDIASPFALGAAAPAAPLPN
                     AYPVPANARLAVSFSAPVAAGQLTINYGDRALGTTALATDQVRLLDWVERAVEVTIQS
                     AIAGTVTLYVLDEWNRPSAIAQGTFA"
     gene            complement(766..1413)
                     /locus_tag="PKN52_04445"
     CDS             complement(766..1413)
                     /locus_tag="PKN52_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99224.1"
                     /translation="MSTKIQLALARLDPANDAHWTSDGSPRLDAIEELAGEKFTRRQV
                     IDAAPQLTRESAAAAAPGQTEQPQAPAIDQFQTGLHAPQEPAQDPEFAQPEPEAKDPV
                     LDNLYAERQSISNQIHRLRKTREDLNREIGELEQAHDKVSKEITAAEPLGSNQAGIQE
                     YLRRQNEIRMARYQRRAAILRDLDAKDFVGKSPLDAAMARKNSRGTQRPKLPPVG"
     gene            complement(1428..1904)
                     /locus_tag="PKN52_04450"
     CDS             complement(1428..1904)
                     /locus_tag="PKN52_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99225.1"
                     /translation="MARNIHARYVRRRSWDRLRRSKNFIDFLTAQAERLKATGALLQF
                     TANSLNGQLTATAHGLVTGAGPILLSNVGGALPLGLVADTPYWVKVVDENTIVLYPTD
                     EDARAGTNPSGFTDNGTGTHSLQRSADAEALFEQYLVAKGKSAEQLLAETDIDDII"
     gene            complement(1960..>2588)
                     /locus_tag="PKN52_04455"
     CDS             complement(1960..>2588)
                     /locus_tag="PKN52_04455"
                     /inference="COORDINATES: protein motif:HMM:NF039452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HNQ99226.1"
                     /translation="LQAATVATMNGVIADNAANDSGDYAVDISGVSFVDGVTNFSAEA
                     FLDAATTMGDSMGDLVAVIVHSVVYNRMQKNNLIDFIPDARGEVNIPTFLGRRVVVDD
                     GMPRTGNVYDTWLFGAGALRLGNGSPKVPTEVDRVPAAGNGGGQEVLYSRIEWSIHPV
                     GHAYVGTPASGGPSNANTANNLAAATSWNRVYAERKQIKFARLVTREA"
BASE COUNT          344 a          856 c          939 g          449 t
ORIGIN      
        1 gaccgcgatc gtcgacctct ggctccagga agagactgac tgtcagggcc tttagagcgt
       61 actcagagac cgccgccttc aggcgatcag gcactccggt gaccagcacc ccgtcgcggg
      121 tgtagaggca cagccgaggg aagctcagca cctgcgtctc gtgctcgcgc cgacccttga
      181 accggccgcc gaaccgctgc tcgatgtagt ccgtcgcctt gatgagggcc gcctccttgg
      241 ccgcctgcgt ggacgaggcc caggaggagt tggcgcggtc ggcgtggtag gtgtcagcga
      301 atgcgacatc gcagaggctg ttggcccctg ccaccccggt cccgtcctcg acagtgaacg
      361 ccatggcccc gccccttacg cgaacgtgcc ctgggcaatc gccgagggcc ggttccactc
      421 atccagcacg tagagcgtga ccgtcccagc gatcgcgctc tggatcgtga cctcgaccgc
      481 ccgctccacc cagtccagca gccggacctg atcagtcgcc agcgccgtgg tgccgagggc
      541 gcggtcgccg tagttgatcg tcagctggcc ggccgcgacc ggggcgctga acgatacggc
      601 gagccgcgcg ttggcgggca ccgggtaggc gttgggcagc ggcgccgccg gggccgctgc
      661 accaagggcg aacggcgagg cgatgtcggg gccgaacttc ttgaagtcgg ccgccgcccg
      721 tcgccgctcc cgtgccttgt agaggacggc cgggcgggcc atggtctacc cgaccggcgg
      781 cagcttcggc cgctgcgtgc ctcggctgtt cttccgggcc atcgccgcgt cgagcggcga
      841 cttgccgacg aagtccttgg cgtcgaggtc gcggaggatc gcagcgcggc gctggtagcg
      901 cgccatgcgg atctcgttct ggcgccggag gtactcctgg atgccggcct ggttcgagcc
      961 caggggctcg gccgccgtga tctccttgga gaccttgtcg tgggcctgct ccagctcgcc
     1021 gatctcgcgg ttgaggtcct cgcgggtctt gcgcagccgg tggatctggt tgctgatcga
     1081 ctgccgctct gcgtagaggt tgtcaaggac ggggtccttg gcctccggct ccggctgggc
     1141 aaactcgggg tcctgggccg gctcctgcgg cgcatggagg cccgtctgga actggtcgat
     1201 ggccggggcc tggggctgct cggtctgccc aggggcagcc gcagctgcgc tctcgcgggt
     1261 gagctgcggg gccgcgtcga tgacctgccg gcgggtgaac ttctcaccgg ccagctcctc
     1321 gatggcgtcg agacggggcg agccgtcgct cgtccagtgc gcgtcgttgg ccgggtccag
     1381 cctggccagg gccaactgta tcttggtgct catgctcctc gtgctcctca gatgatgtcg
     1441 tcgatgtcag tctccgccag gagctgctcg gccgacttgc ccttggcgac caggtactgc
     1501 tcgaacagcg cctcggcgtc cgccgaccgc tgcaggctgt gcgtgcccgt gccgttgtcg
     1561 gtgaagccgg acgggttggt gccagcgcgg gcgtcctcgt cggtcgggta gaggacgatg
     1621 gtgttctcgt ccacgacctt cacccagtag ggggtgtcgg cgacaaggcc caggggcagc
     1681 gcgccgccga cgttggagag gaggatcggg ccagcgcccg tcactaggcc gtgggccgtc
     1741 gcggtcagct ggccgttgag gctgttcgct gtgaactgca ggagggcgcc ggtcgccttc
     1801 agccgctctg cctgagcagt caggaagtcg atgaagttct tgctgcgccg cagacggtcc
     1861 caggaccggc ggcggacgta gcgagcgtgg atgttccggg ccatcgtgat ctcctcgtcg
     1921 aagccagtgg ggagggccgg agccctcccc tctcaagcct tacgcctcgc gggtgacgag
     1981 acgcgcgaac ttgatctgct tgcgctcggc gtacacgcgg ttccacgacg tggcagccgc
     2041 gaggttgttc gccgtgttgg cgttcgacgg gccgccggag gccggggtgc cgacgtaggc
     2101 gtggcccacc gggtggatcg accactcgat acggctgtag agaacctcct ggccgccgcc
     2161 gttgccggcc gccgggacgc ggtcgacctc ggtcgggacc ttcggcgagc cgttgccgag
     2221 gcgcagggcg ccggcaccga acagccacgt gtcgtacacg ttgccggtcc ggggcatgcc
     2281 gtcgtcgacc accacccggc ggccgaggaa ggtcgggatg ttgacctcgc cgcgagcgtc
     2341 ggggatgaag tcgatcaggt tgttcttctg catccggttg tagaccaccg agtgcacgat
     2401 cacggcgacc aggtcaccca tgctgtcgcc catggtcgtg gccgcgtcga ggaacgcctc
     2461 ggcgctgaag ttggtcacgc catcgacgaa ggacacgccg gagatgtcca cggcgtagtc
     2521 gccgctgtcg ttggccgcgt tgtcggcgat gacgccgttc atcgtggcaa cggtggcagc
     2581 ctggagcc
//
LOCUS       DAOQYE010000212         2588 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_43915_length_2588_cov_2.662456, whole genome shotgun sequence.
ACCESSION   DAOQYE010000212 DAOQYE010000000
VERSION     DAOQYE010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2588
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43915_length_2588_cov_2.662456"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..600)
                     /locus_tag="PKN52_04460"
     CDS             complement(<1..600)
                     /locus_tag="PKN52_04460"
                     /inference="COORDINATES: protein motif:HMM:NF015995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HNQ99227.1"
                     /translation="MQKFVLAAIVALVVGSGVANAQTVRFGYVLWDDCVATTNVAAAL
                     IHDRLGYDVDMTSVDAGPMFAGLARGDFDATLCAWLPATHAAYLEEYGDSFEVLNANL
                     EGADIGWAVPAYVDLDSIEDLPGNAGMFNGEVIGIDPGAGLMSASDEAMRAYGLTDYT
                     LLDGSDAAMTAALDRAISRGEPIIVTSWRPHWMWAAYDLK"
     gene            complement(844..1719)
                     /locus_tag="PKN52_04465"
     CDS             complement(844..1719)
                     /locus_tag="PKN52_04465"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline/glycine betaine ABC transporter
                     permease"
                     /protein_id="HNQ99228.1"
                     /translation="MLLDYRIPIASWVERLVRGIQDAWAGGFDGFADVVRFVINGLLG
                     GLQAVPPLILLVALAALVAWAAGWRLALFTAVGLFLIHNIQLWTPFLQTLSLVVTAQV
                     LIVLVGVPLGILAAGSDAAERIMRPVLDFMQTMPAFVYLIPAVMFFGIGLVPGVVATF
                     IFSLPPLVRLVNLGIRQVPVDLVEAADAFGATRLQKLVKVQVPQALPTMMAGLNQSIM
                     LNLSMVVIAGMIGARGLGAEVLRGIQRIQVGQGFEAGLAVVVLAIVLDRLTAAFATRR
                     SKGSRAGGGLTREDG"
     gene            complement(1709..>2588)
                     /locus_tag="PKN52_04470"
     CDS             complement(1709..>2588)
                     /locus_tag="PKN52_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNQ99229.1"
                     /translation="LSGGMQQRVGLARALAMDADILLMDEAFSALDPLIRREMQDELV
                     ELQARVNKTIVFVTHDLDEALKLGDRIAIMRSGRVVQMGGAEEIITAPADDYVEAFVA
                     GVDRTGVLTARSIMQPVKATANVSDGPHTALTKMRRFGLSGLLVIDRERRVRGYAAGE
                     AVVTSMNVREASEANAERLTFAPALTVGLDASLAELITVAAGSSEPIAVVDEGGRLRG
                     VVVKGAILAALASEPPAGAAGAATAATAATTATTAATAATATAGAPDASASGEANADT
                     RGPATRRSGKGAAGAA"
BASE COUNT          413 a          891 c          923 g          361 t
ORIGIN      
        1 cttgaggtcg taagcggccc acatccagtg cgggcgccaa ctcgtgacga tgatgggctc
       61 ccccctcgag atggcgcggt cgagcgcggc cgtcatggcg gcgtcggagc cgtcgagcag
      121 ggtgtagtcg gtgaggccgt aggcccgcat ggcctcgtcg gaggcgctca tcaggccggc
      181 gcccggatcg atgccgatga cctcgccgtt gaacatgccc gcgttgcccg ggaggtcctc
      241 gatgctgtca aggtccacgt aggcgggcac ggcccagccg atgtcggcgc cttccaggtt
      301 ggcgttcagg acctcgaagc tgtcgccgta ctcttcgagg taggccgcgt gggtggcggg
      361 cagccacgca cagagcgtgg cgtcgaagtc gccgcgcgcc aagccggcga acatgggtcc
      421 agcgtcgacg gacgtcatgt cgacgtcgta accgaggcgg tcgtggatga gggcggccgc
      481 gacgttggtg gtcgcgacgc agtcgtccca caacacgtaa ccgaacctga ccgtctgagc
      541 gttggcgacg cccgacccga cgaccagcgc gacaatagcc gctagtacaa acttctgcat
      601 ggttttcctc ctatttttag gagaggcgta aagttgcagt gcggtaacat cccgcaccag
      661 cgcatatatg atgcgccaca cgtacaaggc accacaggcg ccgctttcga ccctagcaca
      721 tgaccaggga gagtgcaaac gcaggcggcg cgccgggcgc ccgcccacgc tgggcgcgcc
      781 cacgctgggc gcgctcacgc tggcctgctc acgctgggcc cacccacgcc gggcgcgccc
      841 gcgtcagccg tcctcccgcg tcaggcctcc ccccgcgcgc gagcctttcg atcggcgcgt
      901 ggcgaaggcg gcggtcagcc ggtcgagcac gatggccagg accacaacgg cgaggccggc
      961 ctcgaagccc tgcccgacct ggatgcgttg gatgcccctc aacacctcgg cgccgaggcc
     1021 gcgcgcgccg atcatgcccg cgatgacgac catcgagagg ttgagcatga tggactggtt
     1081 gaggccagcc atcatggtcg gcagcgcctg cggcacttgc accttgacga gcttctgcaa
     1141 gcgggtggcg ccgaacgcgt cggccgcctc gacgaggtcg acgggcacct gccgtatgcc
     1201 gaggttcacg agccgcacga gcgggggcag cgagaagatg aacgtcgcca ccaccccggg
     1261 cacgaggccg atgccgaaga acatgacggc cgggatcagg tagacgaacg cgggcatggt
     1321 ctgcatgaag tcgaggacgg gccgcatgat gcgctcggcg gcgtccgagc cggcggcgag
     1381 gatgccgagc ggcacgccca ccagcacgat cagcacctgg gcggtcacca cgagcgagag
     1441 cgtctgcagg aacggcgtcc acagctggat gttgtggatg aggaagagcc cgacggcggt
     1501 gaacagcgcc agtcgccaac ccgccgccca ggcgacgagg gcggcgaggg ccactaggag
     1561 gatcaggggc gggacggcct gcaatccgcc gagcaacccg ttgatgacga agcgcacgac
     1621 gtcggcgaag ccgtcgaacc cgccggccca ggcgtcctgg atgccgcgaa cgagcctctc
     1681 gacccacgag gcgatcggga tgcggtagtc aagcagcacc ggcggccccc tttccgctcc
     1741 gccgggtagc gggcccccgc gtgtcggcgt tcgcctcgcc ggaggcgctg gcatcgggag
     1801 cgcccgcggt ggcggtggcc gcggtagccg cagtcgtcgc agtcgtcgcg gccgtcgcag
     1861 ccgtcgcggc acccgcggcg ccggccggcg gctcggaggc caacgcggcc aggatggcgc
     1921 ctttcacgac caccccgcgc agcctgccgc cttcgtcgac gacggcgatg ggttcgctgc
     1981 tgccggccgc gacggtgatg agctcggcca ggctcgcgtc gaggccgacg gtgagggcgg
     2041 gcgcgaaggt caaccgctca gcgttcgcct cagacgcctc ccgcacgttc atgctggtga
     2101 ccaccgcctc accggcggcg tagcctcgca cgcggcgctc gcggtcgatg acgagcagcc
     2161 cggagaggcc gaagcgccgc atcttggtga gcgccgtgtg cggcccgtcg ctcacgttgg
     2221 cggtggcctt gacgggctgc atgatgctcc tcgccgtcag aacgcccgtg cggtcaacgc
     2281 cggccacgaa cgcctccacg tagtcgtccg ccggcgcggt gatgatctcc tccgccccgc
     2341 ccatctgcac cacgcggccg gagcgcatga tcgcgatgcg atctccgagc tttagggcct
     2401 cgtcgaggtc gtgggtgacg aacacgatgg tcttgttcac gcgcgcctgg agctcgacga
     2461 gctcgtcttg catctcgcgg cggatcaggg ggtcgagggc gctgaacgcc tcgtccatga
     2521 ggaggatgtc ggcgtccatg gcgagcgcgc gcgcgaggcc gacgcgctgc tgcatgccgc
     2581 ccgagagc
//
LOCUS       DAOQYE010000213         2574 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_44302_length_2574_cov_2.765383, whole genome shotgun sequence.
ACCESSION   DAOQYE010000213 DAOQYE010000000
VERSION     DAOQYE010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44302_length_2574_cov_2.765383"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            775..2031
                     /locus_tag="PKN52_04475"
     CDS             775..2031
                     /locus_tag="PKN52_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99230.1"
                     /translation="MRIGSPVHVLAAAALLAALASCATPTPPPLPTPALDPAGPLQAK
                     VGSLVHFDTRNLDGPVVWSVNGSVGGSAAVGTVADGDYQAPARVPTDPVVTVAAALAA
                     DPARLADAKVTITAPGTLYVLDAGVYVYNDMDGADGDIAPDRTFQIDGVDPSNDYYGM
                     VVAPSHDAAFIGVQQTGTNVFRVDDVSSASGAVTATGFSTSGRENPAGLAYDVERDIL
                     YVATEYALLAYDDASTAPDGKEPDRVLAGPTVELLVLDYDTRVNLDVANDRLFVSNNN
                     GQVGVYDNASTVDGDVAPDRLVELDAAFSYIWGAAYDARRDELYLADQSEEGVFVIAT
                     VSSINGMVTPARHIAGAATQFLGPSQASYDLANDRLVVIDADADDIKVFDAASTLDGD
                     VAPSRVIGGASLPIDYPYGGYLDPSQ"
     gene            complement(2110..>2574)
                     /gene="deoA"
                     /locus_tag="PKN52_04480"
     CDS             complement(2110..>2574)
                     /gene="deoA"
                     /locus_tag="PKN52_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179292.1"
                     /note="Catalyzes the reversible phosphorolysis of
                     thymidine, deoxyuridine and their analogues to their
                     respective bases and 2-deoxyribose 1-phosphate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HNQ99231.1"
                     /translation="VLTAAGLPASRAALMEAVASGLAYERFERWIAAQGGDVTSLGKL
                     ERAPDAYVVRATRAGVVGKLDALAIGRAAGVLGGGRSKKGEAIDAGVGVELHAKVGDP
                     VAAGDALATAWHRGGRGLEAATALLEDAYEIGESGEPEPLVFERGIGGLTNA"
BASE COUNT          355 a          908 c          900 g          411 t
ORIGIN      
        1 gtgccgttgt aggcgcaaag cgttcgcggc gcgctgttcc agctcggcta gctcggcgcg
       61 gacggcggcg agcgccttct ccctggcgcg ccggcgtttg tccgcgagga ccgcgcgtgc
      121 cacggcgcga cccgcggcct gctcacgcag ttggttcacg cgctcttctg cggcgcggag
      181 tctcgatcgg ctgagctcta gcatgatggc tgccctcctg ggcgctccgg cggtccagtc
      241 ctcggcgggc cagggaagcc aggcggcttc cgaggcgcac ggccgtccga ccggcagacg
      301 gcatgagtag agggatgggt tgctttatag gcgtgactgt cggggaccgt agcgcggcgc
      361 gctgagcgcc gtatgtccta gcggcggcta cttaccgcgc atcagggacg ctggggttcg
      421 gcggctggcg atgacgtcgg cctgctgcgc cccacggacg ggctcatgcg ccgcgctgac
      481 gggcgcgcta tctcctttgg ccatcgctca cctcctcact cctctcgctc tcgcgggcgc
      541 cgcggcagcg ccgccgtggc aagaacgccg cgcgaacgcg gtcgggagct cttcgctccc
      601 aggaggcacg acattagcag ccgggcgggc agatgtcaaa ctcccgcagg cgtccgaagc
      661 ttcacatgcc gccgcgagtc ctcggactat cgtcctagtg gttgttccgt tggtgagttt
      721 gtgccgccca tgcgacgggg ggagagggtg gctcggatca ggaggtactt gcctatgagg
      781 atcggcagcc ccgttcacgt cttagccgca gccgcgctct tggcggcgct ggcgagctgc
      841 gcgaccccga ccccgccgcc gttgcccacg cccgctctcg atccggccgg gccgctccag
      901 gccaaggtcg gttcactcgt gcacttcgac actcgcaacc tcgacggccc cgtggtgtgg
      961 tcggtgaacg ggtcggtcgg cgggtcggcg gccgtcggca cggtcgctga tggcgactac
     1021 caggcgccag ccagggtccc taccgatccg gtcgtgacgg tggccgccgc cctggccgcc
     1081 gatcccgcga ggctggcaga tgccaaggtc accatcacgg cacccggcac cctgtacgtg
     1141 ctcgacgcgg gcgtgtacgt ctacaacgac atggacggcg cggacggcga catcgcgccg
     1201 gatcgcactt tccagatcga tggcgtcgac ccgtctaacg actactacgg catggtcgtc
     1261 gcgccgagcc acgatgcggc cttcatcggg gtccagcaga cggggaccaa cgtcttcagg
     1321 gtcgacgacg tgtcgagcgc ctcgggcgcc gtgaccgcca ccggcttctc gacgtcgggg
     1381 cgcgagaacc ccgccggcct ggcttacgac gtggagaggg acatcctgta cgtagcgacg
     1441 gagtacgcgc tgctggcgta cgacgacgcc tcgacggcgc cggacggcaa ggagccggac
     1501 cgcgtgctgg cgggcccgac cgtagaactc ctcgttcttg attacgacac gcgcgtgaac
     1561 cttgacgtcg ccaacgaccg gctcttcgtc tcgaacaata acgggcaggt cggcgtctac
     1621 gacaacgcgt cgacggtcga cggcgacgtc gcacctgatc gcctcgtgga actcgacgcc
     1681 gcgttcagct acatctgggg cgccgcttac gacgcccggc gcgacgagct gtacttggcg
     1741 gaccagtccg aagagggcgt gttcgtgatc gccaccgtct ccagcatcaa cggcatggtg
     1801 acgcccgcca ggcatatcgc gggcgcggct acgcagttct tagggccttc ccaggcctct
     1861 tacgatctgg ccaacgaccg gctcgtcgtg atcgatgcgg acgcggacga catcaaggtg
     1921 ttcgacgccg cgtcgacgct cgatggcgac gtggccccct cgcgcgtcat cggtggcgcg
     1981 tcgcttccta tcgactatcc gtacggaggc tacctggacc cgagccagtg agcggtaggc
     2041 ccgagccagt gagcgggcac cggattgggt agcgcgtgtc cggccggctg cgggtgggcc
     2101 ggccagacgt catgcgttgg ttaggccgcc gatgccccgt tcgaacacga gcggctcggg
     2161 ctcgcccgac tccccgatct cgtaggcgtc ttcgagcagg gcggtggccg cctctaggcc
     2221 acggccgccc cggtgccagg cggtggcgag ggcgtcgccg gccgctaccg ggtcgccgac
     2281 cttggcgtgc agttcgacgc cgacgcccgc gtcgatggcc tcgcccttct tgctgcggcc
     2341 gccgccaagg acgccggcgg cgcggccgat ggccagggcg tcgagcttgc cgaccacgcc
     2401 cgcgcgcgtc gcccggacca cgtacgcgtc cggcgcgcgc tcgagcttgc ctaggctcgt
     2461 gacgtcgccg ccctgcgccg cgatccagcg ctcgaaccgc tcgtaggcca gaccgctcgc
     2521 gacggcctcc atgagcgccg cgcgcgaagc cggcagcccg gcggccgtga gcac
//
LOCUS       DAOQYE010000214         2570 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_44432_length_2570_cov_2.775348, whole genome shotgun sequence.
ACCESSION   DAOQYE010000214 DAOQYE010000000
VERSION     DAOQYE010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44432_length_2570_cov_2.775348"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1009
                     /locus_tag="PKN52_04485"
     CDS             <1..1009
                     /locus_tag="PKN52_04485"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HNQ99232.1"
                     /translation="PTLAVHAIEVKSLRPAPGVNVVSYSSRESLQGVLAALLKGHARV
                     AMEYSPAGDNPYVGRVDAGTVEWIRSFGVEVVSSGDVAQILEVWTPTQLEQHLTAAAG
                     VCAAKDAAFAYMAEGLARGETVTECAVQGIIMRRFADLGLVSATTPNVSFGAHAGDPH
                     YHPIEGVRDATLAAGDVVLIDLWAKVNDKSAPYADVTWMGVNGEPTAELLSVWEAVKG
                     ARDAAVTAMRSAYAAGRWPSGGEADRASRAVLVASGYGEAFTHRTGHSLGKDGPHGVA
                     VHLDDFETSDARALRPGIGVTVEPGAYFQHFGVRSEINVYLTPEGPRVTTELQDGLER
                     L"
     gene            1016..1975
                     /locus_tag="PKN52_04490"
     CDS             1016..1975
                     /locus_tag="PKN52_04490"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HNQ99233.1"
                     /translation="MERAAVAQAPGLVVVGDYAWDVLIRTNSPLQTGGDVFGEVQLAP
                     GGSVANTAVWAQRCGLQTRFVGKIGNDPFGQLAVDELAAEGLAADLVRSEAHLTGSVA
                     VWIDQEGERSMVSGKGADHYLLPSELPLATLRSAAYVHLHGWSFFGDPPRSAARRAAA
                     VARKAGARVSFDPGSFQMISQMGVEAFLAATTDLGADAVFPNLEEGQVLSGEREPEAI
                     GARLAELYGGALVALKLDASGCYVHERGEGAYFGAAPARLVDSTGAGDSFAGAFLAAW
                     RRGGSAADAARLANRVSAWVVERVGARPRHDAALVELLGGFTG"
     gene            2064..>2570
                     /locus_tag="PKN52_04495"
     CDS             2064..>2570
                     /locus_tag="PKN52_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HNQ99234.1"
                     /translation="MRSRNAKGPAPAGRGTVIAAGARVPFSRAGSDYRELVAYDLARM
                     ALAGLLARADLSGDEVDQVVMGTVVQNVATSNVARDAALAAGVPDGVPAHTVTLACIS
                     SNRAISDAVLAIWAGHAEVVLAGGVEMLGDVPVGFKREVRRRFFDSRRYKGLKDWPAF
                     FKGLKLKDL"
BASE COUNT          346 a          902 c          954 g          368 t
ORIGIN      
        1 cccgacgctg gccgtgcatg ccatcgaggt caagtcgctg cgcccggcgc ccggggtgaa
       61 cgtcgtcagc tattcgagcc gcgagagctt gcagggcgtg ctcgcggcgc tgttgaaggg
      121 ccacgcccgc gtcgccatgg agtacagccc tgccggcgac aacccgtacg tcgggcgggt
      181 tgacgccggc accgtcgagt ggatcaggtc gttcggcgtc gaggtcgtgt cctccggcga
      241 cgtcgcccag atcctcgagg tctggacgcc gacgcagctg gagcagcacc tgacggccgc
      301 ggccggggtc tgcgccgcga aggacgccgc cttcgcctac atggcggaag ggctcgcccg
      361 cggcgagacg gtcacggagt gcgccgtgca gggcatcatc atgcggcgct tcgccgacct
      421 cggcctcgtg tccgcgacca ctccgaacgt gagcttcggc gcgcacgccg gcgaccccca
      481 ctaccacccc atcgagggcg tgagggacgc gacgctcgcc gcgggcgacg tcgtgctcat
      541 cgatctgtgg gcgaaggtga acgacaagag cgcgccttac gccgacgtga cgtggatggg
      601 cgtcaacggc gagcctacgg ccgagctcct gagcgtctgg gaggcggtca agggcgcgcg
      661 cgacgcggcc gttaccgcga tgcgctccgc gtacgcggct ggccgttggc cgagtggcgg
      721 cgaggcggat cgggcgtcgc gcgccgtcct ggtggcgtcc ggctacggcg aggcgttcac
      781 tcatcgcacc gggcacagcc tcggcaagga cggacctcac ggggtcgcag tacacctcga
      841 cgacttcgag acctccgacg cgcgggcgtt gcggcccggc atcggcgtga ccgtcgagcc
      901 cggcgcctac ttccagcact tcggggtgcg cagcgagatc aacgtgtacc tgacccccga
      961 gggcccgcgc gtcacgaccg agctgcagga cgggctcgag cgcctctagg cgagcatgga
     1021 gcgcgcggcg gtggcgcaag cgcccggcct ggtcgtggtc ggggactacg cctgggacgt
     1081 gctcatccgt acaaactcgc cgctccagac gggcggggac gtgttcggcg aggtgcagct
     1141 cgccccaggg ggcagcgtgg ccaacaccgc cgtgtgggcg cagcggtgcg gcctccagac
     1201 gcgcttcgtc ggcaagatcg gcaacgaccc gttcggccag ctcgccgtcg acgagctagc
     1261 ggccgagggg ctcgccgccg acttggtgcg gagcgaagcg cacctgaccg gctcggtggc
     1321 cgtctggatc gaccaggaag gcgagcgctc gatggtctcc ggcaagggcg ccgaccacta
     1381 cctcctgccc tccgaactcc cgttggcgac gctgcgctcg gccgcctacg tgcacttgca
     1441 cggctggtcg ttcttcggcg acccgccacg ttcggcggcg cgcagggcgg ccgccgtcgc
     1501 gcgcaaggcc ggcgcgcggg tgtcgttcga tcccggctcg ttccagatga tctcgcagat
     1561 gggcgtggag gcgttcctgg ccgccacgac cgacctcggc gccgacgctg tcttcccgaa
     1621 cctggaggag ggccaggtcc tctccggcga gcgcgagccc gaggcgatcg gggcgcggct
     1681 cgccgagctc tacggcggcg cgctcgtcgc cctcaagctc gacgcctccg gctgctacgt
     1741 gcacgagcgc ggcgaaggcg cctacttcgg ggccgcgccg gccaggctcg tcgactcgac
     1801 gggggcaggc gactcgttcg ccggggcgtt cctcgccgcc tggcggcgcg gcggttcggc
     1861 cgcggacgcg gcgcggctag cgaaccgtgt ctccgcctgg gtcgtggagc gcgtcggcgc
     1921 gcgcccgcgc cacgacgccg ccctcgtgga gctgctgggt gggttcaccg ggtaggcggc
     1981 ctcggcggct gcgcggatgg ggtatcaagc cccggtggca ccggggcttc agaggcccac
     2041 actgacgtta gactccaggc actatgaggt cacgaaacgc taaaggcccg gcaccggccg
     2101 ggcgcggaac ggtcatagcc gcgggcgcgc gcgtgccttt cagccgcgcc ggcagcgact
     2161 accgcgaact agtcgcctac gaccttgccc gcatggcgct cgccgggttg ctcgcgcgcg
     2221 ccgacctgtc tggcgacgag gtcgaccagg tcgtcatggg caccgtcgtg cagaacgtag
     2281 ccacgagcaa cgtggcgcgc gatgcggctc tcgcggccgg cgtgccggat ggcgttccgg
     2341 cgcacacggt gacgttggcc tgcatctcca gcaaccgcgc gatctcggac gcggtcctgg
     2401 cgatatgggc cggccacgcc gaggtcgtcc ttgccggcgg ggtggagatg ctcggcgacg
     2461 tgccggtcgg cttcaagcgc gaggtgcgcc ggcgcttctt cgatagccgc cggtacaagg
     2521 gcctcaagga ctggccggcc ttcttcaagg gcctgaagct caaggacctg
//
LOCUS       DAOQYE010000215         2570 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_44438_length_2570_cov_2.366600, whole genome shotgun sequence.
ACCESSION   DAOQYE010000215 DAOQYE010000000
VERSION     DAOQYE010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44438_length_2570_cov_2.366600"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="PKN52_04500"
     CDS             <1..412
                     /locus_tag="PKN52_04500"
                     /inference="COORDINATES: protein motif:HMM:NF017743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99235.1"
                     /translation="LFAGGGAISFHLAPAFARVMAVEIDRGAVMRGERDAARLGIANV
                     EFVRQDARQAPVPTGAELVVVDPPRAGLSADVRAAVQASGADRLLYVSCDVATWARDV
                     ADLETRGFRLARFVPYDFYPQTHHVELLSELVRA"
     gene            438..1232
                     /gene="hisF"
                     /locus_tag="PKN52_04505"
     CDS             438..1232
                     /gene="hisF"
                     /locus_tag="PKN52_04505"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HNQ99236.1"
                     /translation="MLAKRIIPCLDVHAGRVTRGQQFGRAELGELADVGDPVALAIAY
                     NDQGADELVMYDITATSEGRGAILDVASRVAERCFMPLTIGGGVRELADMRALMLAGA
                     DKVSINSGAIADPELLARAAEQFGSQAVVLSIDVRARPHGEHGRGGWDVYAAGGRRPT
                     GLDMLAWAERGAALGAGEIVLNSIDGDGMNSGYDLAALRALRAVVGVPVVASGGAGSP
                     EDMRDALMTGVDAALAAGIFHRGEVDVAQVKRVCAAAGLPMREVEA"
     gene            1229..2002
                     /gene="hisIE"
                     /locus_tag="PKN52_04510"
     CDS             1229..2002
                     /gene="hisIE"
                     /locus_tag="PKN52_04510"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF002747.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HNQ99237.1"
                     /translation="MTDTGTASGRNTGGGVGVADIGYDERGLVPVVVQSTADGAVLML
                     AYANRQALSLTLETREAHYYSRSRSEIWRKGATSGNTQRVVAVRYDCDADALLYLVEE
                     RGPACHTGERTCFYRALGEGDADPGARGEAHGLGAAGGPDATVGASAASTGATPAEAA
                     GADRLGRALAALQSVIAERLVTLPEGSYVRKLHERGVGYVAQKVVEEAGETVVAALLQ
                     RDDELVAESADLLFHLGVLLTERGVSLGRVADKLDERRR"
     gene            2058..>2570
                     /locus_tag="PKN52_04515"
     CDS             2058..>2570
                     /locus_tag="PKN52_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99238.1"
                     /translation="MRRPFVASAVALALVLAGVAFVSCAPAASSDDLARAGATRTTSS
                     GSPLDGIGYVPVNAPSSTLRPRPDDAPDLVIIAFSGHCGLICDTRSTWSYLSNASEST
                     GGVAVLDAIARAYARLGLAKVEVFSASSFATSHYSSISGKIEPGYLQAQAYLDEVKRD
                     WIDGVADPTRV"
BASE COUNT          345 a          868 c          966 g          391 t
ORIGIN      
        1 gctcttcgcg ggtggcgggg cgatctcctt tcacctcgca ccggccttcg cgcgcgtcat
       61 ggccgtggag atcgaccgcg gcgccgtcat gcggggggag cgcgatgcgg ccaggctcgg
      121 catcgcgaac gtcgagttcg tgcggcagga cgcgcggcag gcgccggtgc cgaccggcgc
      181 ggagctggtg gtcgtcgacc cgccgcgcgc cggcttgtcg gcggacgtgc gcgcggccgt
      241 ccaggcctcc ggcgccgacc ggctcttgta cgtcagctgt gacgtggcga cgtgggcgcg
      301 ggacgtcgcc gacctcgaga cgcggggctt ccggctcgcg cgtttcgtgc cttacgactt
      361 ctacccgcag acgcaccacg tcgaactcct gtcggagctg gtgcgcgcct gaggccggcg
      421 gggctacact cgtgcacgtg cttgccaaac ggatcatccc ttgcctggac gtccacgccg
      481 gccgtgtgac ccgcggtcag cagttcggac gcgccgagct gggtgagctc gcggacgttg
      541 gcgaccccgt cgccctcgcc atcgcctaca acgaccaggg ggccgatgag ctcgtcatgt
      601 acgacatcac cgcgacgagc gaaggtcggg gcgccatcct cgacgtggcc tcgcgcgtgg
      661 ccgagcggtg cttcatgccc ctgaccatcg gtggcggcgt gcgggagttg gcagacatgc
      721 gggcgctcat gttggccggg gcggacaagg tctccatcaa ctctggcgcc atcgccgacc
      781 cggaacttct cgcgcgcgcc gccgagcagt tcggttcgca ggccgtggtc ctctcgatcg
      841 acgtgcgggc caggcctcac ggggagcatg gacggggggg ctgggatgtc tacgcggccg
      901 ggggcaggcg ccccaccggc ctcgatatgc tcgcatgggc cgagaggggc gcggcgttgg
      961 gagcgggaga gatcgttctg aacagcatcg acggcgatgg catgaactcc ggctacgacc
     1021 tggcggccct gcgcgccctg cgagcagtgg tgggcgtacc ggtggttgct tcagggggcg
     1081 ccggcagccc cgaggacatg cgcgacgccc tcatgaccgg ggtagatgct gcgctcgcgg
     1141 cgggcatctt ccaccgcggt gaggtggacg tggcgcaggt caagcgcgtc tgcgcggcgg
     1201 ccggcctgcc catgcgcgag gtggaggcat gacggatacg gggacggctt caggcaggaa
     1261 cacgggcggc ggcgtcggcg tggccgacat cggttacgac gagcgtgggc tcgtgccggt
     1321 cgtggtgcag tcgacggccg acggcgccgt cctgatgctg gcctacgcta accgccaggc
     1381 cctgtcgctg actctcgaga cccgcgaggc gcactactac agccgctcgc gctccgagat
     1441 ctggcgcaag ggcgcgacga gcggcaacac gcagcgtgtg gtggcggtcc gttacgactg
     1501 cgatgcggac gcgctgctct acctggtcga ggagcgcggc cccgcctgcc acaccgggga
     1561 gaggacgtgc ttctaccggg cgctgggcga gggcgacgcc gatcccggcg cgcgtggcga
     1621 ggcgcacggt cttggcgccg ctggtgggcc ggacgcgacc gtaggtgcat cggccgcttc
     1681 gaccggcgca acgcccgccg aagcggccgg agccgaccgc ttgggtcggg cgctcgcagc
     1741 cctgcagtcg gtgatcgcgg agcgcctcgt gacgttgccc gagggctctt acgtgcgcaa
     1801 gctccacgag cgcggggtcg gctacgtggc gcagaaggtc gtcgaggagg cgggcgagac
     1861 ggtcgtggcc gccctactgc agcgcgacga cgaactggtg gccgagagcg ccgacctcct
     1921 gttccacctc ggcgtgctcc tgacggagcg cggggtgagc ctcggtaggg tcgccgacaa
     1981 gctggacgag cgtcggaggt agcgcggtct cgtgaggcta cccaaatagg tggctcaagt
     2041 cgtaggctcg ttggcgcatg cgtcgcccct tcgtcgcctc ggccgtagcc ctcgccctcg
     2101 tgttggcggg cgtggcgttc gtgtcctgcg cgcccgccgc ctccagcgac gacctggcgc
     2161 gggccggcgc gactcgcacc acgtcgagcg gctcgccgtt ggacggtatc ggctacgtgc
     2221 cggtcaacgc gccgagttcc accctgcgtc cgaggcccga tgacgcgcca gacctggtca
     2281 tcatcgcgtt ctccggacac tgcggcctca tctgcgacac gcgcagcacg tggtcgtacc
     2341 tgagcaacgc tagcgagtcg acgggcgggg tggccgtgct agacgccatc gcgcgcgcct
     2401 acgcgcgcct cggcctggcg aaggtcgagg tcttcagcgc ctcgtcgttc gccacgagcc
     2461 actacagctc catcagcggg aagatcgagc ccggttactt gcaggcgcag gcctacctcg
     2521 acgaggtgaa gcgcgactgg atcgacggcg tcgccgaccc gacgagggtg
//
LOCUS       DAOQYE010000216         2566 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_44540_length_2566_cov_2.662684, whole genome shotgun sequence.
ACCESSION   DAOQYE010000216 DAOQYE010000000
VERSION     DAOQYE010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44540_length_2566_cov_2.662684"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /gene="pilM"
                     /locus_tag="PKN52_04520"
     CDS             complement(<1..289)
                     /gene="pilM"
                     /locus_tag="PKN52_04520"
                     /inference="COORDINATES: protein motif:HMM:NF022554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HNQ99239.1"
                     /translation="MVSFRERFARMLSPKIEAVGLEVGTSALKVVELRAGNPPTLSAL
                     AVRPMPAGLVSEDEVTDQQGLAEEIKALFQEAGIQKRFTVTAVSNRQAITRN"
     gene            complement(423..2213)
                     /gene="dnaB"
                     /locus_tag="PKN52_04525"
     CDS             complement(423..2213)
                     /gene="dnaB"
                     /locus_tag="PKN52_04525"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HNQ99240.1"
                     /translation="METRTPPHNIEAEQSVLGSILLDNEVFANLEGTLHAEQFYKEAH
                     RKVFRAMEKLHRRGEPLDLVTLTEELRQAGDLEGVGSVAYLVGLVDSVPTAAYAESYA
                     RIVQEKATLRDLIGAGGRIMQAAYDQAGPLEDVLDQAEKAIFDLTTRKRRSNFEGMRT
                     LVTETFQDINERFANPNPVSGLRAGFRDLDGMTGGLQPASLNVLAARPSMGKTAFALT
                     IGLNAALKEAASVGIFSLEMSAVQLVTRMLCAEARVDMSRVRSGQLSDRDFQRLADTA
                     GRMSEARVFIDDHADMTVMELRSRARRLQAEHGLDLLIIDYLQLMSGSSRSQSENRQQ
                     EISAISRGLKGLARELEVPVLVLSQLSRAVEARPNKRPMLSDLRECVSGDTLVDLADG
                     TRVPIAELVGRQPELFAMDVSGKIVKAKSDKVWSVGAKELFEVRLASGRVRRTTARHL
                     WYSFDGWKRLEQLAPGDRVAIARRLPHTEPVSGVANAAVAYDSDLYWDEIVGISSIGM
                     HEAYDLTVPGPASWLADGVVMHNSGAIEQDADLVMFIYRDEYYDPHSEKHGIAEIIIG
                     KQRNGPTGSLELQFHNAHVRFNDLAKGGHG"
BASE COUNT          348 a          850 c          907 g          461 t
ORIGIN      
        1 tgttacgggt gatggcctgg cggttgctca ccgccgtgac ggtgaaccgc ttctggatgc
       61 cggcttcctg gaagagcgcc ttgatctctt cggctagtcc ctgttggtcg gtgacctcgt
      121 cctcgctgac gaggccagcc ggcatcggtc tgacggccag cgcgctcagc gtgggcgggt
      181 tgcccgcgcg gagctccacc accttgagtg cgctggtccc gacctcgagg ccgaccgctt
      241 cgatcttggg acttagcatg cgagcgaagc gctcgcgaaa tgagaccata gcgtcccctc
      301 ctagtaaatc tgggggagtc tagcacgcga ccttcgcgcc accacgtgaa ataatgccgc
      361 acagaccatc gcgctccggg cattcaccca agggcagtgg gctccgtgcc aggcgcccct
      421 tctcagccgt ggccaccctt ggcgaggtcg ttgaaccgca cgtgcgcgtt gtggaactgg
      481 agctcgaggc tgcccgtcgg cccgttgcgc tgcttgccga tgatgatctc ggcgatgccg
      541 tgcttctcgc tatgcgggtc gtagtactcg tcgcggtaga tgaacatgac caggtcggcg
      601 tcctgctcga tcgcgcccga gttgtgcatc acgacgccgt cggcgagcca cgacgccggg
      661 cctggaaccg tcaggtcgta cgcctcgtgc atgccgatgc tgcttatgcc gacgatctcg
      721 tcccagtaca ggtcggagtc gtaagccacg gcagcgttcg ccacgccact gaccggctcg
      781 gtgtgcggta gccggcgtgc gatggcgacc ctgtcgccgg gcgccagctg ctctaggcgc
      841 ttccagccat cgaagctgta ccagaggtga cgggcggtgg tgcgcctgac gcgcccgctg
      901 gctaggcgga cctcgaacag ttccttagcg ccgaccgacc acaccttgtc gctcttggcc
      961 ttgacgatct tgccgcttac gtccatggcg aagagctcgg gctgtcggcc gacgagctcg
     1021 gcgatgggga cgcgtgtgcc gtcggcaagg tcgacgaggg tatctccgct cacgcactca
     1081 cgcaggtccg acagcatcgg ccgcttgttg ggcctggcct cgacggcgcg cgagagctgg
     1141 ctgaggacga gtacgggcac ctcgagctcg cgcgccagcc ccttgaggcc gcgcgagatg
     1201 gccgagatct cctgctggcg gttctcgctc tggctgcggc tgctgcctga catgagctgg
     1261 aggtagtcga tgatcaggag gtcgagcccg tgctccgcct gcaggcgcct ggcgcgcgag
     1321 cgcagctcca tgacggtcat gtcggcgtgg tcgtcgatga agacgcgggc ttcggacatg
     1381 cggccggccg tgtcggccag gcgctggaag tcgcggtcgg agagctggcc cgacctcacc
     1441 cttgacatgt ccacccgggc ctcggcgcag agcatgcgcg tcacgagctg cacggccgac
     1501 atctcgaggg agaagatgcc gacggaggcg gcttccttga gtgcggcgtt caggccgatg
     1561 gtgagcgcga aggccgtctt gcccatcgac ggccgggcgg ccagcacgtt caggctcgcg
     1621 ggctgcagcc cgcccgtcat gccgtccagg tcgcggaagc cagcgcgcag cccggacacg
     1681 gggttcgggt tggcgaaccg ctcgttgatg tcctggaacg tctcggtgac gagcgtgcgc
     1741 atgccctcga agttcgagcg gcgcttgcgc gtcgtgaggt cgaagatggc cttctcggcc
     1801 tggtcgagca cgtcctcgag cggcccggcc tggtcgtagg ccgcctgcat gatgcggccg
     1861 ccggccccga tgaggtcgcg gagggtcgcc ttctcctgca cgatgcgcgc gtagctctcc
     1921 gcgtaagccg ccgtcggcac ggagtcgacg aggccgacga ggtaggcgac gctgccgacc
     1981 ccctcgaggt cgccggcctg gcgcagctcc tcggtgaggg tgacgaggtc cagcggctcg
     2041 ccgcgccggt gcagcttctc catggcgcgg aagaccttgc ggtgggcctc cttgtagaac
     2101 tgctcggcgt gaagggtgcc ctcgaggttg gcgaacacct cgttgtcaag caggatgctg
     2161 cccaggacgc tctgttcggc ttcgatgttg tgcggcgggg tcctggtctc caccgggtca
     2221 tcttaccggt cgacctcggg taggtaggcg cgcgccgggt ggcgccacct ggtcgtgtcc
     2281 cggaccctga cgcgcccccg ccgttgggcg gtcccgctgg cgagggcagc gccgggcgtc
     2341 gggccggtga cgcgcccccc gccgtcgggc ggtccgccgt cgtgtcgtcg cagaccgggc
     2401 cgcgcggcgg aggccaacgg atagaatggc gccattgtta tggaactcct gactgcctgg
     2461 gccttgccgt tactgagcgc atatctggtg ggctcgctgc cgttcggtgc gtggctggtg
     2521 caggccgcgt cgggcttcga cccgcgcgat gtcaacccgc acctcc
//
LOCUS       DAOQYE010000217         2564 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_44593_length_2564_cov_2.396572, whole genome shotgun sequence.
ACCESSION   DAOQYE010000217 DAOQYE010000000
VERSION     DAOQYE010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44593_length_2564_cov_2.396572"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="PKN52_04530"
     CDS             <1..408
                     /locus_tag="PKN52_04530"
                     /inference="COORDINATES: protein motif:HMM:TIGR01554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HNQ99241.1"
                     /translation="LDGVELGDEGRAVWCANRSIIPKLYAIKDGAINQLFIPNAYGIG
                     PKPTPTLLGIPIVWTEKCSALGTKGDLILADWSYYLIGDRQQVVVDWSDRVAFLNLQS
                     TVRLYERVDGKPWMDATHTPRKGAARSPFVILT"
     gene            422..1204
                     /locus_tag="PKN52_04535"
     CDS             422..1204
                     /locus_tag="PKN52_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99242.1"
                     /translation="MARPKLNERLKVDVGLIPQTINNNNATGDYYDVRGWEKIRAIVM
                     DGASVVNKTTKVEFLQATDAAGTGAKVVKQSNASTGTESSATSVAAAAKLSAVTECTV
                     ALSSAGNGETLTITDTDGTDYVFTGHTNTTTASKREFAVDGTDAQDCTALVGLINHAT
                     YGVPGITAVDGSSGTMTLKVSDKGEGTFSVATSAVAHFVPAITKQVLISEVDVLDLDL
                     SGGFYYVAVKITKAGNGIVGAVLERTHAQRLPEQAAAASTVL"
     gene            1322..1948
                     /locus_tag="PKN52_04540"
     CDS             1322..1948
                     /locus_tag="PKN52_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99243.1"
                     /translation="MADPTALFTETDARAFNGAALADSSRYPTAEITAVEATIRAEFA
                     RLCRIQFIPTSTTVVLNGCASSSIRLPLHNPLREQPPRYLSITAASIDGVALTAAELA
                     DLAAEPNGRVTRKTLGSWLGAAPNIGNVSITARHGYATVPGDIKRAALVVLLAQLLPS
                     EIAPNATSYNDGMMTYQIGYAGDWPHPYGLHEVDAVLLRYREERVTIA"
     gene            1945..2424
                     /locus_tag="PKN52_04545"
     CDS             1945..2424
                     /locus_tag="PKN52_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99244.1"
                     /translation="MSGVTVTFGIAAALKDAFAVNAALSAIDVTSGPVAGERDPREWI
                     QIGRPRVTRTPRTITGQPMGGHDEDGTVPVMVLASADGRGEEAIVRLRERMEELIGAV
                     EAALAADFTLGGIVRWAYVSALAEQDQGIEPPPAAGHWMTVELTVSFAARVYPGVAT"
BASE COUNT          443 a          923 c          826 g          372 t
ORIGIN      
        1 cttgatggcg tagagcttgg cgatgagggc cgggcggtgt ggtgcgccaa ccgctccatc
       61 atccccaagc tctacgccat caaggacggc gccatcaacc agctcttcat cccgaacgcc
      121 tacgggatcg gccccaagcc cacgccgacc ctgctcggca tcccgatcgt ctggaccgag
      181 aagtgctcgg cgctcggcac caagggcgac ctgatcctgg ccgactggag ctactacctg
      241 atcggcgacc gccagcaggt cgtggtcgac tggtccgacc gtgtcgcgtt cctcaacctc
      301 cagagcaccg tgcgtctcta tgagcgcgtc gacggcaagc cctggatgga cgccacccac
      361 acgcctcgca agggcgcagc ccggtcaccg ttcgtcatcc tcacctgaga ggcggtgtgt
      421 aatggcacgg cctaagctca atgagcggtt gaaggtcgac gtcggcctga tcccgcaaac
      481 catcaacaac aacaacgcga ccggcgacta ctacgacgtc cgcggctggg agaagatccg
      541 cgccatcgtc atggatggcg cctcggttgt gaacaagacc accaaggtcg agttcctgca
      601 ggccaccgac gcggccggca ccggcgccaa ggtggtcaag cagtccaacg catccaccgg
      661 caccgaatca tcggcgacca gcgtagccgc cgcggccaag ctgtcggccg tgaccgagtg
      721 cacagtcgcg ctgtcgagcg cgggtaacgg tgagaccctg accatcactg acaccgatgg
      781 tacggactac gtcttcactg gtcataccaa cacgaccacc gcgtcgaagc gcgagttcgc
      841 ggtcgacggt accgatgccc aggactgcac ggcgctggtc ggcctgatca accacgccac
      901 ctacggcgtg cccggcatca cggcggtcga tggcagctcc ggcaccatga cgctcaaggt
      961 ctccgacaaa ggtgaaggca ccttctcggt cgccaccagc gcggtcgccc acttcgtccc
     1021 ggcgatcact aagcaggtgc tcatcagcga ggtcgacgtg ctggatctcg acctgagcgg
     1081 cggcttctac tacgtcgccg tgaagatcac caaggccggc aacggcatcg tcggcgccgt
     1141 gctggagcgt acccacgctc agcgcctgcc ggagcaggcg gcagcggcga gcaccgtcct
     1201 gtaggaccga tggtgcgcag cggacagatc ggttccgggc gcacgagccg cacgggggcg
     1261 gcgggttccg gcccgccgcc ctctgcgcgg ctgtcggtca gacgaaaggc acgggtatcg
     1321 gatggctgac ccgaccgcac tgttcactga gacggatgcg cgggccttca acggcgccgc
     1381 gcttgccgac tcgtcccgct atccgaccgc cgagatcacg gcggtggagg cgaccatccg
     1441 cgccgagttc gcgcgactct gccggatcca gttcatcccg accagcacga ccgtagtcct
     1501 caacggctgc gcctcatcga gcatccgcct cccgctgcac aacccgttgc gggaacagcc
     1561 gccgcgctac ctgagcatca ccgctgcgag tatcgatggc gttgcgctga ccgcggccga
     1621 gcttgccgac ctagcggcgg agccgaacgg gcgcgtcacg cgcaagacgc tcggctcctg
     1681 gctcggcgct gcaccgaaca tcggcaatgt ctcaatcacc gcccggcacg gctatgcgac
     1741 cgttccggga gacatcaagc gggcagcgct ggtcgtgttg ctggcgcagc tgctcccgag
     1801 cgagatcgcg cccaacgcga ccagctacaa cgacggcatg atgacctacc agatcggcta
     1861 tgccggcgac tggccacacc cctacggcct gcacgaggtc gacgccgtgt tgctgcgcta
     1921 ccgcgaggaa cgggtgacga tcgcatgagc ggcgtcaccg tcaccttcgg gatcgccgcc
     1981 gcactcaagg acgccttcgc cgtcaacgcc gcgctctccg ccatcgacgt caccagcggg
     2041 ccggtggcag gcgagcgcga cccgcgcgag tggatccaga tcggccggcc gcgggtgacc
     2101 cgcacgccac gcaccatcac cggccagccg atgggcggcc acgacgagga cggcaccgtg
     2161 ccggtcatgg tgctggccag cgccgacggc cgcggcgagg aggcgattgt gcgcctgcgc
     2221 gagcgcatgg aggaactcat cggcgcagtc gaagccgcgc tggcggccga cttcactctc
     2281 ggcggcatcg tgcgctgggc ctacgtctcg gcgctcgccg agcaggatca gggcatcgag
     2341 ccgccgccgg ccgccggtca ctggatgacg gtcgagttga ccgtctcgtt cgccgcccgc
     2401 gtctacccag gagtcgccac atgaagctga cctacaccgg cacggagccg gctgccgtgc
     2461 cggacgccat cgctgcggtg caccccgccc agcaggctct ggcggcggcg cagggcagct
     2521 gcgctgttgc tggcaccagt gccttggcgc ccgccgcctc gctc
//
LOCUS       DAOQYE010000218         2562 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_44631_length_2562_cov_3.144795, whole genome shotgun sequence.
ACCESSION   DAOQYE010000218 DAOQYE010000000
VERSION     DAOQYE010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44631_length_2562_cov_3.144795"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..628)
                     /locus_tag="PKN52_04550"
     CDS             complement(<1..628)
                     /locus_tag="PKN52_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99245.1"
                     /translation="MPAPLTPDSFTLVRFAEPGAFAALAEPFMLEREAANCLQLGLMP
                     AVVDGRFEDVYQAVLEGPDGEVVMVALQTPPNRLILTDAVIAGSPVRGSDDAATAAVA
                     EALVRGLLEPTDGQPVELPGVLGPVALAEAFARAWGERNGVPVRRTVQERVYVCTEVT
                     RVSGVPGAMVKAGLDQVDLLVDLVARFREEAQVLICTEAGGEGRNMQFC"
     gene            700..1701
                     /locus_tag="PKN52_04555"
     CDS             700..1701
                     /locus_tag="PKN52_04555"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNQ99246.1"
                     /translation="MPVPGPSAPASEAASSPEASTPQIKVGERRHKGTGVVRRSTPAT
                     NLAVGTSLMLAAMVLLPMMDGLAKGLSERLPVPMIIWARYFFHLLCLLPLLLLRFGPR
                     ALVPRQLGLQLVRGSLLLAATVLFFFALAMLPQATALALFFVSPLVVTLLAPALLGER
                     VGPWRVVAVLVGLLGIILILRPGSGAFGWGAALALTAGVVHALYMVFTRHLAGSAPPL
                     LTLGYTAVVGAVVMTLVLPFVWQRPALADFGVLALLGVLAAGGHFLLIKAFDYAPATW
                     LAPVGYSEMVTAVIYGYLSYGFVPDALGWLGLAIVVGAGVAISLKERRAMRGLRPGN"
     gene            complement(1805..2320)
                     /locus_tag="PKN52_04560"
     CDS             complement(1805..2320)
                     /locus_tag="PKN52_04560"
                     /inference="COORDINATES: protein motif:HMM:NF019241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1572 family protein"
                     /protein_id="HNQ99247.1"
                     /translation="MTQSADQTDLGTVLIADIVRGYRAQKLQAERALDQLEAADWHRT
                     LDEDGNSIAVIVQHVSGNLRSRWTDFLTSDGEKPDRDRDGEFEARRRMPEELLAAWDE
                     AFAVVFGTLAALTPADLAKTITIRDQPLGVVSAILRNFEHTGHHVGQIVLLAKHWRGP
                     EWRTLSIPKKR"
BASE COUNT          331 a          929 c          886 g          416 t
ORIGIN      
        1 tgcagaactg catgttgcgc ccctcgccgc ccgcctcggt gcagatgagc acctgcgcct
       61 cctccctgaa gcgcgcgacg aggtccacga gcaggtcgac ctgatcgagg cccgccttca
      121 ccatggcgcc cggcacgccg ctcacccgcg tcacttcggt acagacgtag acgcgctcct
      181 gcacggtcct gcgcaccggc acgccgttgc gctcgcccca ggcgcgcgcg aacgcctccg
      241 ccagcgcgac gggcccgaga acgcctggga gctcgacggg ctggccgtcg gttggctcga
      301 gcagcccgcg caccagcgcc tctgccaccg cagccgtggc agcgtcatca gagcctcgca
      361 cggggctccc cgcgatgacc gcgtcggtca ggatgagccg gttgggcggc gtctgcagcg
      421 cgaccatcac gacctcgccg tccggacctt ccaggaccgc ctggtagacg tcctcgaagc
      481 ggccgtcgac gacggccggc atgagaccca gttgcaggca gttggccgcc tcccgctcca
      541 gcatgaacgg ctcggcgagc gcggcgaacg cgcccggctc ggcgaagcgc acgagggtga
      601 aggagtccgg cgtgaggggg gctggcacgg gccgatgata tgcgagcgga gcgtccgacg
      661 ggacgagcag gaaccgcgtc gctcacatat catctcctgg tgcccgttcc cggcccgtcc
      721 gcccccgcct ccgaagccgc gagcagcccg gaggcgtcga caccgcagat caaagtcggc
      781 gaaaggcgcc acaagggaac gggcgtggtc cgtcgcagca cgcccgccac gaacctcgcc
      841 gtcggcacga gcctgatgct ggccgccatg gtgctgctgc ccatgatgga cggccttgcc
      901 aagggcctct ccgagcgttt gcccgtgccg atgatcatct gggcgcgcta cttcttccac
      961 ctgctctgcc tgctgccgct gctcctcctg cgcttcggtc cgcgtgcgct cgtcccacgc
     1021 cagcttgggc tccagctcgt caggggttcg ctgctgctgg ccgccaccgt cctcttcttc
     1081 ttcgcgctgg ccatgctgcc ccaggccacg gccctcgcgc tgttcttcgt atcgccgctg
     1141 gtcgtcacct tgctcgcgcc cgcgctcctg ggcgaacgcg tcggcccatg gcgggtcgtg
     1201 gcggtgctcg tcggcctcct cgggatcatc ctgatcctca ggcccggctc cggcgcgttc
     1261 gggtggggcg cggcgctggc gttgacggcg ggcgtggtgc acgccctcta catggtcttc
     1321 acgcggcacc tggcaggcag cgcccctccg ctgctgacgc tcggctacac ggcggtggtc
     1381 ggggcggtcg tcatgacgct cgtcctaccc ttcgtgtggc agcgaccggc gctggccgac
     1441 ttcggcgtcc tcgcgctcct aggcgtgctc gcggccggtg gccacttcct gctcatcaag
     1501 gcgttcgact acgccccggc cacgtggctg gcgccggtgg gctattccga gatggtcacg
     1561 gccgtcatct acggctactt gtcgtacggc ttcgtgcctg acgcgctcgg ctggctcgga
     1621 cttgccatcg tggtcggcgc cggcgtggcc atctccctca aggagcggcg cgccatgcgg
     1681 gggctgcgcc ccggcaactg acgcgggagt cggggtccgg agtcggggtc cggtgccgcg
     1741 accgccacgc cgaggcgcga gcccatcccg cagccgcgcc gagcctctgc gcttcggcct
     1801 ggcctcagcg cttcttcggt atggacagtg tgcgccattc cgggccgcgc caatgcttgg
     1861 caagcaagac gatctgcccg acatggtggc ccgtgtgctc gaagttgcgc aggatggccg
     1921 atacgacacc aaggggttgg tccctgatcg tgatcgtctt ggcgaggtcg gcgggcgtga
     1981 gcgcggccag cgtgccgaac actacggcga aggcctcgtc ccaggcggcg agcagctcct
     2041 ccggcatccg gcgccgcgct tcgaactcgc cgtccctgtc gcggtctggc ttctcgccgt
     2101 cgctcgtcag gaagtcggtc cagcgggagc gcaggttgcc actcacgtgc tgcactatca
     2161 ccgcgatgga gttgccgtcc tcgtcgaggg tgcgatgcca gtcggccgcc tcgagctgat
     2221 ccaaggcacg ctcggcctgg agcttctggg cgcggtaacc gcgcacgatg tcggcgatga
     2281 ggacggtccc gaggtcggtc tggtctgcgc tctgcgtcat gggcagatgg taacgccgac
     2341 accacgttcc cacgccgcag gcgcctccgc ccgcgaggct ccgagtcgcg ccgaaggaag
     2401 caaccgacct ggtggtggtc gggcggtagg agggcgccga cgcgggtgcc tcggtaagat
     2461 gcggccatgg cgaagaagga gcaaggcgcg gccaggctgg ccgccgtagc gaccgcgcgc
     2521 ctgagcaacg tggccgccgg cctggtcgac ctggtccgcg ac
//
LOCUS       DAOQYE010000219         2560 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_44683_length_2560_cov_3.619162, whole genome shotgun sequence.
ACCESSION   DAOQYE010000219 DAOQYE010000000
VERSION     DAOQYE010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44683_length_2560_cov_3.619162"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="PKN52_04565"
     CDS             <1..385
                     /locus_tag="PKN52_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNQ99248.1"
                     /translation="LVRAIVYASMNQSPLNRQTVARALSDVFAPGDINLTMPDILKRT
                     AEHFGVKPDDVRGKGRRQELVVPRQVAMYLIRELTTHSYPEIGQYFADRDHSTVMYAV
                     QKIEGIIQEESDLSRAVRTLRESLV"
     gene            863..1954
                     /gene="dnaN"
                     /locus_tag="PKN52_04570"
     CDS             863..1954
                     /gene="dnaN"
                     /locus_tag="PKN52_04570"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HNQ99249.1"
                     /translation="MKVLVPKKSLADTLARVERIVPSRSSNPGLSLLRIDVVEDGMEL
                     SGSNMDVDIRARFAVDAPARGSYAVVGHVFSQVVRALPGEDVQLDLGDDEMQISSGSY
                     STKLQLMSPSSAPVLNFGTRFDGGIDAAQLARALSSVKYAASVADFQAVFRGVKLELA
                     DGRTRAVATDGFRLAYYDLKESSGLNTEVIVPARSVDELLRVLGDGEVKLALEPGQLS
                     VQHGNYSLNLKLMDGTFPDYERVIPKTFPVSITLDAARLAEAVARVALMADKTTNNRV
                     DLFVKGGELRITAEGSFGRSQEALSVMQEGTDPEIGLAYNSKYLTDALSPADGEVRLS
                     FSGSNSAPSVVTDLGDSSYLAMVVPLRTS"
     gene            1974..>2560
                     /gene="eno"
                     /locus_tag="PKN52_04575"
     CDS             1974..>2560
                     /gene="eno"
                     /locus_tag="PKN52_04575"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HNQ99250.1"
                     /translation="MTIIEDVRALEVLDSRGNPTVAAVVTLTSGATGTALVPSGASTG
                     AHEAVELRDGGSRYAGKGVLKAVQNVNERIAPELIGYDALEQAAVDRAMCELDGTTNK
                     SELGANAILAVSLATARAASEAVELPLYRYLGGPNGRVLPVPLMNVINGGKHADNAVD
                     MQEFMLVPLGFSTFGSALRAGVETFHSLKKVLSKRG"
BASE COUNT          493 a          820 c          825 g          422 t
ORIGIN      
        1 gctggtgcgc gccatcgtct acgcgtccat gaaccagtcg ccgctcaacc gccagaccgt
       61 ggcgcgcgcc ctctcggacg tcttcgcgcc gggcgacatc aacctgacga tgccggacat
      121 cctcaagcgc accgccgagc acttcggtgt caagccggac gacgtgcgag gcaagggcag
      181 acggcaggaa ctcgttgtgc cgcgccaagt ggccatgtac ctgatccgcg agctgaccac
      241 gcactcctat cccgagatcg ggcagtactt cgccgaccgc gaccattcga ccgtcatgta
      301 cgcggtacag aagatcgagg gcatcatcca ggaggagagc gacctcagca gagcggtccg
      361 caccctgcgc gagtctctcg tatgaaaccg ccagcggacc cccgaagccc ggtcagctgt
      421 tgataaccct gtggataagc ttgtagacaa gctgtcgaac atgctgtgaa gaaccattcg
      481 aggctcgggg ctgttgaggg tagctgacaa gtagcggacc gttccacagg gagcctgtac
      541 tagtccacag ttcggtccac agcccagata ccgtgcctca cgcgtggcgt cgcgttatcg
      601 acagtctcca cagggcctac taggtaggag ggtgaggctt tcctttaggt tgaaacctag
      661 caagagtaat cagtggtggc cgaggaggcc cgtgggtcta ccatatggaa acctcgccag
      721 gaagggctta acaaggtatg ctctgacacc gggcgcagcc ttcgacagag tgtggaggca
      781 accggttcgt gttacccacc cctcggaagg tacccatggt gacgaacgag gagcgcgtgg
      841 agaaggaacc caggagagtc gcatgaaggt gctcgtcccg aagaagagcc tcgccgacac
      901 actcgcacgc gtcgaacgca tcgtgccgag ccggtcaagc aaccctggcc tgagcttgtt
      961 acgtatagac gtcgtcgagg atggcatgga actcagcggg tcgaacatgg atgtcgatat
     1021 ccgcgcgcgc ttcgcggtcg acgcgccggc caggggaagc tacgccgtcg tgggccatgt
     1081 gttcagccag gtggtacgcg cccttccggg cgaggatgtc caactggacc tgggcgacga
     1141 cgagatgcag atcagctccg gctcctactc cacgaagctg cagctgatgt cgccatcgtc
     1201 ggcgccggtg ctcaacttcg gtacccggtt cgacggcggc atcgacgcgg cgcagttggc
     1261 ccgggcgctc tcgtccgtga agtacgcggc ctcggtagcc gacttccagg cggtgttccg
     1321 cggggtcaag ctggaactgg ccgacggtcg cacgcgcgcg gtggcgacgg atggcttcag
     1381 gctcgcctac tacgacctca aggaaagcag cggtctcaac accgaggtga tcgtgcccgc
     1441 ccgcagtgtc gacgaactcc tccgggtgct cggtgacggc gaggtaaagc tggcgctcga
     1501 gcccggtcag ctttccgtcc agcacgggaa ctactccctc aacttgaagc tcatggacgg
     1561 gacgttcccc gactacgagc gtgtcatccc caagaccttc cccgttagca tcaccctgga
     1621 cgctgcgcgc ctcgccgagg ccgtagccag ggtcgctctc atggccgaca agacgaccaa
     1681 caaccgcgtc gacctgttcg ttaagggcgg cgagttgcgc atcacggcag agggcagctt
     1741 cggtcgcagc caagaggcgc tgtcggtgat gcaggagggc acggatccgg agatcggcct
     1801 ggcctacaac agcaagtacc tcacagacgc cctctccccc gcggatggcg aggtgcggtt
     1861 gagcttctcc ggctccaact ccgccccgag cgtggtcacg gacctcggcg acagcagtta
     1921 cctcgccatg gtcgtaccgc tcaggacttc gtaaccctgt tccaggagga acgatgacga
     1981 tcatagaaga cgtgcgcgcg ctcgaggtgc tcgactcgcg cggtaatccg accgtggctg
     2041 ccgtggtcac gctgaccagc ggcgccaccg ggacggcgtt ggtgccgtca ggcgcgagca
     2101 cgggcgccca tgaggccgtc gagctccgcg acggcggcag caggtacgcc ggcaagggcg
     2161 tactgaaggc cgtgcagaac gtgaacgagc gcatcgcgcc cgagttgatc ggttacgacg
     2221 cgctcgaaca ggcggcggtc gaccgcgcga tgtgtgagtt ggacgggacc acgaacaagt
     2281 cagaactcgg cgccaacgcc atcctcgccg tctccctcgc gaccgcgcgc gcggcctccg
     2341 aagcggtcga actacccctc taccgttact tgggcggccc gaacggtcga gtgctgccgg
     2401 tgccgctgat gaacgtcatc aacggcggca agcacgccga caacgcggta gacatgcaag
     2461 agttcatgct ggttccgctc ggcttctcca ccttcggcag cgcgttgagg gcgggcgtcg
     2521 agacgttcca cagcctgaag aaggtcctct ccaagcgcgg
//
LOCUS       DAOQYE010000220         2560 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_44695_length_2560_cov_2.585230, whole genome shotgun sequence.
ACCESSION   DAOQYE010000220 DAOQYE010000000
VERSION     DAOQYE010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44695_length_2560_cov_2.585230"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1830)
                     /locus_tag="PKN52_04580"
     CDS             complement(<1..1830)
                     /locus_tag="PKN52_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ99251.1"
                     /translation="MAGAAEDAKGAEAAHRAAEEGQRGLLTSAANRAAAGAARRPPDL
                     LDTDLPDYSVEEAKAAYRTRDRERRRAMRAESVRDEDGEEFEKAYDQGIMVRLLGYLR
                     PYRLQLSVGVGLLLLSSALTPVFPTLLARALDDYIIGTAEPFASLDIDGRLRGLTVIV
                     AIYLALRLLSFGIRFGYTYLVSWLGQRIVYDMRREMFAKIQRLHMGFFDRTPVGRLIT
                     RITSDVDAIQQMMTDGVVGLIADVGLMGGLLFYMFTINWRMALITLVVMPPLFFILNL
                     LRRRIRTAYRSVRLRTSRSNAYLAENLSGMKTVQLFNREARNARRFDELNLQLLDANN
                     EQVRWFSLFFPTIGITSAISTALILYYAGTQLVPDVASFVTIGTLVAYIQYSGMFFRP
                     LQDLSDKFNIIQAAMASSERIFGLLDTPEQIADLPGAAGFESSFRGEVAFEDVWFAYD
                     DEEWVLRGVDFTIAPGESVAFVGHTGAGKTTIISLVSRFYDVQKGRVTIDGKDVREYD
                     QVQLRKHVGIVLQDPFLFSGTVRSNITLGDDSVPQERVEEAARFVNAHGFISRLPEGY
                     DTPVLERGAGFSTGQKQLIAFARALVQNPDILLVLDEATANVDT"
     gene            complement(1830..>2560)
                     /locus_tag="PKN52_04585"
     CDS             complement(1830..>2560)
                     /locus_tag="PKN52_04585"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ99252.1"
                     /translation="CYPGSNQAVLKKLSFRLQAGEKVGIIGRIGSGKTTIEKLVLGLY
                     QPTEGAILIDGIDNKRFPVEVLRRFIGLVPQEPFLFSDSIADNIAYGLPEAPAEVLAP
                     RVLRAAEIAQLDGDVQEFPSGFDTRLGERGVTLSGGQRQRTAIARALVREPSILIFDD
                     ALSAVDTQTEARILEGLADVQRGRTTVIVAHRISAFQNAHRVLVLDNGRVVEEGTHDE
                     LIALGGWYADMDRRQQLESDLEVA"
BASE COUNT          410 a          823 c          909 g          418 t
ORIGIN      
        1 ggtgtcgacg ttggccgttg cctcgtccag gaccaggagg atgtcggggt tctgcaccag
       61 ggctcgggcg aacgcgatga gctgcttctg gcccgtcgag aagcccgccc cgcgctcgag
      121 gacgggcgtg tcgtaaccct cgggcaggcg cgagatgaac ccgtgcgcgt tgacgaaccg
      181 cgccgcctcc tcgacgcgct cctgcggcac gctgtcgtcg ccaagggtga tgttgctgcg
      241 caccgtgccg gagaacaaga acgggtcctg cagcacgatg ccgacgtgct tgcgcaactg
      301 cacttggtcg tactcgcgga cgtccttgcc gtcgatcgtc acgcgcccct tctgcacgtc
      361 gtagaagcgg ctgacgaggc tgatgatcgt cgtcttgccc gcgccggtgt gtccgacgaa
      421 cgccaccgac tcgccgggcg cgatggtgaa gtcgacaccg cgcaacaccc actcctcgtc
      481 gtcgtacgcg aaccagacgt cctcgaacgc cacctcgccc ctgaacgaac tttcgaagcc
      541 ggccgctcct ggcaggtcgg cgatctgctc cggcgtgtcg agcagaccga agatgcgctc
      601 gctcgaggcc atggcggcct ggatgatgtt gaacttgtcc gagaggtctt gtagcggcct
      661 gaagaacatg ccggagtact ggatgtatgc caccagcgtg ccgatggtga cgaacgacgc
      721 gacgtctggc acgagctgcg tgccggcgta gtacaggatc agcgcggtcg agatggcgct
      781 cgtgatgccg atggtcggga agaagaggct gaaccaacgc acctgctcgt tgttggcgtc
      841 gagcagctgg aggttgagct catcgaagcg ccgcgcgttg cgggcctcgc ggttgaagag
      901 ctggacggtc ttcatgccgc tcaggttctc cgccaggtag gcgttcgagc gcgaggtcct
      961 gaggcgcacg gagcggtaag cggtccggat gcggcgcctg agcaggttca ggatgaagaa
     1021 gagcggcggc atgaccacca gcgtgatgag cgccatgcgc cagttgatgg tgaacatgta
     1081 gaagagcagt ccgcccatca gtccgacgtc ggcgatcagc ccaacgacgc cgtccgtcat
     1141 catctgctgg atggcgtcca cgtcgctggt gatgcgcgtg atcaggcgcc cgaccggggt
     1201 gcgatcgaag aagcccatgt gcagccgctg gatcttggcg aacatctcgc gccgcatgtc
     1261 gtagacgatg cgctgcccca gccacgacac gaggtaggtg tagccgaacc tgatgccgaa
     1321 gctcagcagc ctgagggcca ggtagatggc gacgatcacc gtgaggccgc gcaggcggcc
     1381 gtcgatgtcg aggctggcga acggctccgc cgtcccgatg atgtagtcgt cgagcgcgcg
     1441 cgcgagcagg gtggggaaga ccggcgtcag ggcgctgctg agcagcagga gcccgacgcc
     1501 gacggagagc tggagccggt acggcctgag gtagccgagg agccgcacca tgatgccctg
     1561 gtcgtaggcc ttctcgaact cctcgccgtc ctcgtctcgc acgctctcgg cgcgcatggc
     1621 gcggcgccgc tcgcggtcgc gcgtgcggta ggcggccttc gcctcctcga cagagtagtc
     1681 gggcaggtcg gtatccagca ggtcgggcgg gcggcgggcg gcgccggccg cggcacggtt
     1741 cgcggcgctg gtcaagagcc cgcgctgacc ttcctcagcg gcgcggtgcg cggcctcggc
     1801 tcccttggca tcctccgctg cgcccgccat cacgccacct ccaggtcgga ttcgagttgc
     1861 tgcctacggt ccatgtcggc gtaccaaccg cccaaggcga tgagctcgtc gtgcgtgccc
     1921 tcttctacga cccggccgtt gtcgagcacg agcacgcggt gcgcgttctg gaacgcgctg
     1981 atgcggtggg cgacgataac ggtcgtacga ccccgctgga catcggccag cccctcgagg
     2041 atgcgggcct cggtctgcgt gtcgacggcc gagagggcat cgtcgaagat gaggatggac
     2101 ggctcgcgca ccagcgcgcg ggcgatggcc gtacgctgcc gctggccacc ggagagcgtg
     2161 accccgcgct cgccgagacg cgtgtcgaag ccgctgggga actcctgcac gtcgccgtcc
     2221 aactgcgcga tctcggcggc gcgcaggacg cgcggcgcga ggacctcggc gggggcctcc
     2281 ggcaggccgt acgcaatgtt atcggcgatg ctgtcggaga agaggaacgg ctcctgcggc
     2341 acgaggccga tgaaccgcct gaggacctcg acggggaagc gcttgttgtc gatgccgtcg
     2401 atgaggatcg ccccctcggt gggctggtag aggcccagca cgagcttctc gatggtggtc
     2461 ttgcccgagc cgatgcggcc gatgatgccc accttctcgc cggcctggag gcggaagctc
     2521 agcttcttga gcaccgcctg gttgctgccc gggtagcaga
//
LOCUS       DAOQYE010000221         2559 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_44710_length_2559_cov_3.544728, whole genome shotgun sequence.
ACCESSION   DAOQYE010000221 DAOQYE010000000
VERSION     DAOQYE010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44710_length_2559_cov_3.544728"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1299
                     /locus_tag="PKN52_04590"
     CDS             <1..1299
                     /locus_tag="PKN52_04590"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF006053.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HNQ99253.1"
                     /translation="HRQDIDEAARWLAARFERAGVPDVRILPTKGHPVVVARWHHDES
                     APTVIIYGHYDVQPPEPLELWNSQPFEPEVRDGRIYGRGASDDKGGVMIALHAVEASA
                     SATGAPPVNVTFLVEGEEEIGSPNLAPFLAENKDLLAADLAISADGGIFGVGIPSVTV
                     GSRGLAGVEVTVTGANADLHSGMYGGAVANPIMALARILASMQDADGRILIDGFFDGI
                     PALNAELRAAIAAVPEEGAGASSLGLDGWWGDPAYTPAERRVARPTLEINGMWGGYQG
                     GGIKTVLPKEAHAKITCRLVVGQEPDRVVAALTEHIRRNTPAGVKVDVRPLAGKGRAY
                     LMPLDHPVLAVASEAMEAAYGKRPFPTFTGGTVPVAEQFGSVLGMWCLYFAFGEPDNG
                     LHAPNEFFRLSAMEKGTEATVRLLHALAATGKDALRAPLV"
     gene            1313..>2559
                     /gene="hpaB"
                     /locus_tag="PKN52_04595"
     CDS             1313..>2559
                     /gene="hpaB"
                     /locus_tag="PKN52_04595"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HNQ99254.1"
                     /translation="MPARDGQQFLDGLRRDPPNLYVDGERVLDPTAHRATSAAARAIA
                     DLYDMQLSGETGCDMTFASPSTGEPVGLSFIEPLTKDDLRARSDMHRAWARYSLGFIG
                     RSPDYLNVNLMACARAAHYFARGGERFAENVRRYFEHVRERDLCLTHALTDPQVNRAA
                     PSDRLHDPYIALGVVRETSAGVVVRGARMLATLPVADELLILPPPAGSTAVEPQRYAL
                     AFAVPCSTPGLTFIAREPLEERRNLSDHPLGARFDELDALVTFDDVLVPWERVFLLGD
                     ADLAAGLYSSTNAAYQMGHQVLNVKIVKAEAFVGVAELIVAAIGSGRFEHVQLKLAEL
                     VTVLEVLKALKTQAEEFATPDEYGTMTPDKAALATARAYFPQVYPRLVEILQLLGASG
                     LIMLPSERERQGRAVAIASRRILV"
BASE COUNT          384 a          954 c          868 g          353 t
ORIGIN      
        1 cataggcagg acatcgacga ggccgcgcgc tggctggcgg cgcgcttcga gcgcgccggg
       61 gtgcccgacg tgcgcatcct gccgaccaag ggtcacccgg tcgtcgtcgc ccgctggcac
      121 cacgacgaga gcgcgccgac cgtcatcatc tacggtcact acgacgtgca gccgcccgag
      181 ccgctcgagc tgtggaactc tcagccgttc gagccggagg tacgcgacgg gcgcatctac
      241 ggtcgtggag ccagcgacga caagggcggg gtgatgatcg ccctccacgc cgtcgaggcc
      301 agcgcatccg ccacaggcgc gccgccggtc aacgtcacct tcctggtcga gggcgaggag
      361 gagatcggca gcccgaacct cgcgcctttc ctcgcggaga acaaggacct cctggcggcc
      421 gatctggcca tcagcgccga cggtggcatc ttcggcgtcg gcatcccgtc cgtgaccgtc
      481 ggctcgcgcg gcctcgcggg cgtcgaggtg accgtcacgg gcgccaacgc cgacctccac
      541 tccggcatgt acggcggggc cgtcgccaac ccgatcatgg cgctcgcacg catcctcgcg
      601 agcatgcagg acgccgacgg gcggatcctc atcgacgggt tcttcgatgg catcccggcg
      661 ttgaacgccg agctgcgcgc cgccatcgcc gccgtgccgg aagagggcgc cggagcctcc
      721 agcctgggtc tcgacggctg gtggggcgat cccgcctaca cgccggccga gcgccgcgtc
      781 gccaggccga ccctcgagat caacggcatg tggggcggtt accagggcgg cggcatcaag
      841 accgtgctac ccaaggaagc gcacgccaag atcacctgcc gcctcgtagt cgggcaggag
      901 ccggacaggg tcgtggcggc gctgacggaa cacatccgtc gcaacacccc cgccggcgtg
      961 aaggtggacg tcaggccgct cgccggcaag ggccgcgcgt acctcatgcc cctcgaccac
     1021 cccgtcctgg ccgtggccag cgaggccatg gaggcggcgt acggcaagcg ccccttcccg
     1081 accttcactg gcgggacggt ccccgtggcc gagcagttcg gcagcgtcct cggcatgtgg
     1141 tgcctctact tcgcgttcgg ggagcccgac aacggcctgc acgcccccaa cgagttcttc
     1201 cgcctctcgg ccatggagaa gggcaccgag gcgacggtgc ggctcctcca cgccctcgcc
     1261 gcgaccggca aggacgcgtt gcgggccccg ctcgtctgaa ggccgggcga ccatgccggc
     1321 gcgcgacggg cagcagttcc tcgacggcct gaggcgcgac ccgccgaacc tctacgtcga
     1381 cggtgagcgc gtcctcgacc cgaccgcgca ccgcgccacg tcggccgctg cccgcgccat
     1441 agccgacctg tacgacatgc agctgagcgg ggagacgggc tgcgacatga cgttcgcgag
     1501 cccctccacc ggggagcccg tcggtctgag cttcatcgag ccgctgacca aggacgacct
     1561 gcgcgcgcgc tcggacatgc accgcgcctg ggcacgctac tcccttggct tcatcggtcg
     1621 cagccccgac tacctcaacg tgaacctcat ggcgtgcgcg cgggccgcgc actacttcgc
     1681 ccgcggcggc gaacggttcg cggagaacgt gaggcggtac ttcgagcacg tgcgcgagcg
     1741 cgacctctgc ctcacgcacg ccctcacgga cccgcaggtc aaccgcgccg cgccgagcga
     1801 ccggctgcac gacccgtaca tcgcgctggg cgtcgtgcgt gagacgagcg cgggcgtcgt
     1861 cgtgcgcggc gcgcgcatgc tcgcgacgct ccccgtggcg gacgagctcc tgatcttgcc
     1921 gcctcccgcc ggaagcaccg ccgtggagcc ccagcgctac gccctcgcgt tcgccgtgcc
     1981 gtgctcgacc ccgggcctga ccttcatcgc gcgcgagccc ctcgaggagc gccgtaacct
     2041 cagcgaccac cccttgggtg cccgcttcga cgagctggac gccctcgtga cgttcgacga
     2101 cgtgctggtg ccgtgggagc gagtgttcct gctcggcgac gccgacctgg ccgccggcct
     2161 gtactcatct accaacgccg cctaccagat gggccaccag gtcctgaacg tcaagatcgt
     2221 caaggcggag gccttcgtcg gggtggcgga gctcatcgtg gcggccatcg gcagcgggcg
     2281 cttcgagcac gtgcagctca agctggccga gctggtgacc gtgctggagg tcctcaaggc
     2341 cctaaagacg caagcggagg agttcgccac ccccgacgag tacggcacca tgacgccaga
     2401 caaggccgcg ctcgcgacgg cgcgggcgta cttcccgcag gtctacccgc gcctggtcga
     2461 gatcttgcag ctcctcggcg ccagcggcct gatcatgttg ccaagcgagc gcgagcgcca
     2521 gggccgagcc gtcgcgatag cgtcgcgccg gatccttgt
//
LOCUS       DAOQYE010000222         2559 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_44717_length_2559_cov_2.923722, whole genome shotgun sequence.
ACCESSION   DAOQYE010000222 DAOQYE010000000
VERSION     DAOQYE010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44717_length_2559_cov_2.923722"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(14..1678)
                     /locus_tag="PKN52_04600"
     CDS             complement(14..1678)
                     /locus_tag="PKN52_04600"
                     /inference="COORDINATES: protein motif:HMM:NF014610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HNQ99255.1"
                     /translation="MDKLFITIRPTAEVTIKSRTTRTVFLRKLRLAVKDALQRSGLEA
                     RAFVRGNRVMAEVTGGRGSAVAGGGGSGAGNDVESTGTLSRRVTHAEPVDTGLASTLA
                     TSALERVFGIGTFSFVEAVAAPDLAAIVALGTELYAERVRGTTYAVRCKRVGRHAFGS
                     MDVERKLGAALNPGAKVDLKNPEVTVEIEVDERQALFFSRRHHGPGGLPLGTGGHALA
                     LLSGGFDSIVAAWSLMRRGVEVDFVHFRLGDLESERLALRVAKLLSDTWGSGSRPEAH
                     VIDLRPAMREMRDNLDTNLWQVGLKRLMVKAADGVADALEAHASTSAPGEEGPRQRGR
                     RTSRRRVDALVTGEAIGQVSSQTLSNLRTIDAAATRPVLRPLIAHDKQEIISLAERIG
                     TAELSAKAVEECNITPVRPATSSRTERLERQEGGMDGGALAGQLARVERGDTRIPLRA
                     MREGEGVLVEEAAGDDLRVSALPTGAVLIDCRDEGIRSWLPDWPETISVPSDLSGGGL
                     EPGRVYVAFCPMGQRSAYVAERLRVGGVRAHSFKGGEGALRAYLEG"
     gene            complement(1961..>2559)
                     /locus_tag="PKN52_04605"
     CDS             complement(1961..>2559)
                     /locus_tag="PKN52_04605"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNQ99256.1"
                     /translation="DLRVAVNLSASQVARPELVDEVGAALAAAGLPPSALEIEVVERA
                     AMTDVVGSARKLSALKRMGVRVALDDFGVEYSSLAYLRSFPIDALKVDGVFVRGLGDE
                     TALAKASRKGAHPDADGAIVRAIVTLGNSMGLRVVAEGIETLTQWRAVRALGCGEAQG
                     FLICRPAPPEALGSMLARGRVDGWDELAVSDPPPDWQP"
BASE COUNT          374 a          956 c          874 g          355 t
ORIGIN      
        1 caggccgagg ccctcacccc tcgaggtacg cccgcaaggc gccctcgccc cccttgaagc
       61 tatgggcgcg cacgcccccc acgcgcaggc gctccgccac gtaggcgctg cgctgaccca
      121 tggggcagaa cgcgacgtac acgcgtccgg gctcgagccc gccgcctgac aggtcgctcg
      181 gcacggagat ggtctcgggc cagtccggca gccacgagcg tatgccctcg tcgcgacagt
      241 cgatcaggac agcgcctgtc gggagggctg acacacgcag gtcgtcgccc gcggcttcct
      301 cgacgagcac gccctcgccc tcgcgcatgg cgcgcagcgg gatacgggtg tcgccccgtt
      361 cgacccgcgc cagctggccg gcgagcgcgc cgccgtccat gcccccctcc tggcgttcca
      421 accgctccgt gcggctcgag gtggccggcc gcacgggcgt gatgttgcac tcctcgacgg
      481 ccttggcgga gagctcggcg gtgccgatgc gctccgccaa ggagatgatc tcctgcttgt
      541 cgtgggcgat gagcggcctg agcaccgggc gcgtcgcggc ggcgtcgatg gtgcgaaggt
      601 tggagagcgt ctgggacgac acctgcccga tggcctcgcc cgttaccagg gcgtcgacgc
      661 gccggcgcga ggtgcgcctg ccacgttggc gcggtccctc ctcgcccggt gcgctcgtcg
      721 aggcgtgcgc ctccagcgcg tccgccacgc cgtcggccgc cttcaccatc aggcgcttga
      781 ggccgacctg ccagaggttc gtgtccaggt tgtcgcgcat ctcgcgcatg gccggccgca
      841 ggtcgatgac gtgagcctcg gggcgcgagc cggaacccca cgtgtcggag aggagcttgg
      901 ccacgcgcag agcgagccgc tccgactcga ggtcgcccag gcggaagtgg acgaagtcga
      961 cctcaacgcc gcgccgcatc agcgaccagg cggccacgat cgagtcgaaa ccgcccgaca
     1021 ggagcgcgag cgcatgaccg cccgtgccga ggggcagacc ccccgggccg tggtgacgcc
     1081 ggctgaagaa gagcgcctgg cgctcgtcga cctcgatctc cacggtcacc tcggggttct
     1141 tgagatcgac cttggcgccg gggttgaggg cggcccccaa cttgcgctcg acgtccatcg
     1201 acccgaacgc atgccggccc acgcgcttgc aacgcacggc gtaagtggtg ccacgtacgc
     1261 gctccgcgta gagctcggtg cccaaggcca cgatggcggc gaggtctggg gcggccaccg
     1321 cctcgacgaa cgagaacgtg cctatcccga acacgcgctc caacgcgctg gtggccaggg
     1381 tgctggccag gccggtgtcg accggctccg catgggtcac gcggcgcgac agggtgccgg
     1441 tcgactccac gtcgttcccg gcgcccgacc cgccgccacc ggccacggcg gagccacgcc
     1501 cgccggtcac ctcggccatg acgcggttgc cacgcacgaa cgcgcgcgcc tccaggcccg
     1561 agcgctgcaa cgcgtccttg acggccagcc ggagcttgcg gaggaagacg gtgcgcgtgg
     1621 tgcgcgactt gatagtcacc tccgctgtag gacggatggt tatgaacagt ttgtccatcg
     1681 gaacttccct cacgtgatcg actatgacgc gaggaggtcc ccgccgcgcc gctctcttca
     1741 tcaaggataa agccccgcga gtcgtaaaga taaagccccg cgcggtagtc gccggttccc
     1801 ccgaaccctg gcgttcccgc gcggttgaga cagggttaca caccaccgcg acagctagcg
     1861 tcgctgcgtt ccgggctcgt acaacggcca ccagccgggc cccgagaccc ggcggcgcat
     1921 ccggttcgtc gcgtgtgcgg cgctgccggc gacgctcgcc ctagggttgc cagtccggcg
     1981 gcgggtcgct gacggcgagc tcgtcccacc cgtctacgcg accacgcgcc agcatgctac
     2041 cgagcgcctc gggcggagcc gggcggcaga tgaggaagcc ttgcgcctcc ccgcagccca
     2101 gggctcgcac cgcgcgccac tgagtcaacg tctcgatgcc ctcggcaacc acccgcaggc
     2161 ccatgctgtt gccgagagtg acgatggccc ggacgatggc gccgtccgcg tccggatggg
     2221 cccccttgcg ggacgccttg gcgagcgccg tctcgtcccc caaccccctt acgaacaccc
     2281 catccacctt gagggcgtcg atagggaagc ttcgcagata ggcgaggctc gagtactcga
     2341 ccccgaagtc gtccagcgcg acccgcacgc ccatccgctt gagcgcggag agtttcctgg
     2401 cgctgccgac cacgtcggtc atggccgcgc gctccaccac ctcgatctcg agggccgagg
     2461 gcggcaggcc ggcggcggcg agggccgctc ccacctcgtc gaccagctcg gggcgggcca
     2521 cctggctcgc gctcaggttc acggccacgc gcaggtcgg
//
LOCUS       DAOQYE010000223         2550 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_44946_length_2550_cov_3.733868, whole genome shotgun sequence.
ACCESSION   DAOQYE010000223 DAOQYE010000000
VERSION     DAOQYE010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2550
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44946_length_2550_cov_3.733868"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(201..926)
                     /locus_tag="PKN52_04610"
     CDS             complement(201..926)
                     /locus_tag="PKN52_04610"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HNQ99257.1"
                     /translation="MRETRRALEVATIELTLESGIENVTIEQIAERATVSPRTFFNYF
                     PSKEDALLGLASERWEADLLAAFPRRPSGAGVYIDLRDFLVAHFRERLFADDLLERRI
                     AALASSPELMKRNLNQMTVLLQKLTLLTARLLLAEGTRAHEGNGSEVGGPVEDRAVAE
                     GPIPDEHAHPAAALPPEPTAEAHMLLLVCGSALNYAFHSWLTSGTGDQPADGIASAFA
                     RLEKTLVRQVLPVVGREASDPTP"
     gene            complement(1122..2087)
                     /locus_tag="PKN52_04615"
     CDS             complement(1122..2087)
                     /locus_tag="PKN52_04615"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HNQ99258.1"
                     /translation="MSAEAPASAATLPRHLLDFAAWSAADVVALLDRADVMAQVLTRT
                     IKKVPALQGFTVATLFFEESTRTRLSFERAARAQSADVISFAAGGSSLKKGESLRDTL
                     RTVDQLQVDLYVVRHPAGGAPYQLARWTDAAIVNAGDGRRAHPTQALLDAYTIRKRFG
                     ASGERPFAGRRLTIVGDIDHSRVARSNVELFTKLGGEVTLCGPATLVPAEFGALPGVR
                     VVARLVEAVEGADAVMGLRLQQERMSAGLLPSVSEYVARYQLTEAVMRHAREDAILMH
                     PGPLIRDVEVDSALADGPRSVIEEQVANGVPVRMAVLYSLLVGKR"
     gene            complement(2084..>2550)
                     /gene="pyrR"
                     /locus_tag="PKN52_04620"
     CDS             complement(2084..>2550)
                     /gene="pyrR"
                     /locus_tag="PKN52_04620"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HNQ99259.1"
                     /translation="ERNKGAERLALVGIHTRGVPIAERLSTYIAEYEGVTPPFGKLDI
                     TLYRDDLTEIALQPIVRKTEVDFDVHGMRIVLCDDVLFTGRTARAGLDALIDMGRPAA
                     IQYAVVVDRGHRELPIRADYVGKNVPTSSLEVVQVKLAEVDGVNAVELWERA"
BASE COUNT          371 a          854 c          916 g          409 t
ORIGIN      
        1 catgagcgtc gacttgcccg agccgctctt cccgacgatg gcgacggact cgcccgccgc
       61 gacggcgatg gacacccctt tgagggcctc gaagtagctc tctccgctgc cgtatgactt
      121 cgtgatgtcg tgtgcttcca ggatctgaat ggcctggctc gctttcgggt cccgccgcga
      181 cggtggtcgg gtcggggtgg ctagggggtg gggtcggagg cttccctgcc gacgacgggg
      241 aggacctgcc ggaccaaggt cttctccaga cgagcgaagg cggaggcgat gccgtcggcc
      301 ggttggtcgc ccgtgccgga cgtcagccag gagtggaacg cgtagttgag ggcggagccg
      361 cacacgagca ggagcatgtg ggcctccgcc gtgggctctg gcggcagggc ggctgcggga
      421 tgcgcatgct cgtctgggat ggggccttcg gcaacggcgc ggtcctccac cgggccgcca
      481 acttccgaac cgttaccttc atgagctctc gtaccctcgg ccagcaggag ccgagccgtg
      541 aggagggtga gcttctggag cagcacggtc atctggttga ggttgcgttt catgagttcg
      601 gggctcgagg cgagcgccgc gatacgcctc tcgagcaggt cgtcggcgaa gaggcgctcc
      661 cggaagtgcg caacgaggaa gtcgcgcagg tcgatgtaga cgccggcgcc gctcggccga
      721 cgcgggaagg cggccagcag gtcggcctcc cagcgttcgg aggcgagacc caacagcgcg
      781 tcctccttgg aggggaagta gttgaagaag gtgcgcggcg agacagtggc gcgctccgcg
      841 atctgctcga tcgtgacgtt ctcgatgccc gactccagcg tgagctctat cgttgcgacc
      901 tcgagggcgc ggcgcgtctc gcgcatcttc ctctctctca ggccggttgt cccgacatca
      961 tccacgaggg cgaatgattg cacaccgtga aacgatgcgc aataggcaat gattcaccgt
     1021 aggtgggggc gtcgcgaccg gcctgccccc ggtccctgac tcgtccgaga gggcgacccc
     1081 cgtgatgcct gccgtctcgg cgtcgcgacc ctgaaggctc gtcagcgctt gccgaccagg
     1141 agcgagtaca gcacggccat gcgcaccggc acgccgttgg cgacctgctc ctcgatgaca
     1201 ctgcgtggac cgtccgccag cgcgctgtcg acctccacgt cgcggatcag ggggcccgga
     1261 tgcatgagga tggcgtcctc gcgcgcgtgc cgcatgacgg cttccgtgag ctggtagcgc
     1321 gccacgtact cgctcaccga ggggagcagg ccggcgctca tgcgctcctg ctgcagccgc
     1381 agccccatga cggcgtccgc cccttcgacc gcctccacga gccgcgcgac gacgcgcacg
     1441 cctggcaggg caccgaactc cgccggcacg agcgtggccg ggccgcatag cgtgacctcg
     1501 cccccgagct tcgtgaagag ctcgacgttc gagcgcgcca cgcgcgagtg atcgatgtcg
     1561 ccgacgatcg tgaggcgtct gcccgcgaac ggccgctcgc cgctcgctcc gaagcgcttc
     1621 cggatcgtgt aggcgtcgag gagtgcctgc gtcgggtggg cgcggcgacc gtcgccggcg
     1681 ttgactatgg ccgcgtccgt ccagcgcgcg agctggtacg gggcgccgcc agccgggtgg
     1741 cgcaccacgt agaggtcgac ctgcagctga tcgacggtcc gcagcgtgtc gcgcaggctc
     1801 tcgcccttct tgagcgagga gccgccggcc gcgaagctga tgacgtccgc gctctgcgcc
     1861 ctcgcggcgc gctcgaagct cagccgtgtg cgcgtgctct cctcgaagaa gagcgtggcg
     1921 accgtgaagc cctgcagggc gggcaccttc ttgatggtgc gcgtgaggac ctgcgccatg
     1981 acgtccgccc tgtccagcaa ggccaccacg tccgccgccg accaggcggc gaagtcgagg
     2041 agatggcggg ggagggtcgc ggcggaggcg ggcgcctcgg cgctcacgcg cgctcccaga
     2101 gctcgacggc gttcacgcca tcgacctccg cgagcttcac ctgcacgacc tcgagcgagg
     2161 acgtcggcac gttcttaccg acgtaatcgg ctcggatggg cagctccctg tggcccctgt
     2221 cgacgacgac cgcgtactgg atggccgccg gtcgccccat gtcgatgagg gcgtcgaggc
     2281 cggcgcgggc ggtgcggccc gtgaacagca cgtcgtcgca gagcacgatg cgcatgccgt
     2341 gcacgtcgaa gtcgacctcg gtcttcctga cgatgggctg cagggcgatc tccgtcaggt
     2401 cgtctcggta cagggtgata tccagcttgc cgaagggtgg cgtcaccccc tcgtactcgg
     2461 cgatgtaggt ggagagacgc tccgcgatgg gcacgccgcg cgtgtggatg ccgacgagcg
     2521 cgaggcgctc cgcgcccttg ttgcgctcga
//
LOCUS       DAOQYE010000224         2538 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_45278_length_2538_cov_3.430125, whole genome shotgun sequence.
ACCESSION   DAOQYE010000224 DAOQYE010000000
VERSION     DAOQYE010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2538
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45278_length_2538_cov_3.430125"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..601
                     /locus_tag="PKN52_04625"
     CDS             <1..601
                     /locus_tag="PKN52_04625"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNQ99260.1"
                     /translation="RSFVQALAQGPLRGTQTAKEPDPAVRSTLQRNHLRTSGGSVDNA
                     AIIEAICAMAHKLNKEVVAEGVETAYQRDFLRSLGADNAQGYYFAKPMLPSQAEELLK
                     RVTVEAAMAQTAAEARASAVKELRSSAVREPWMPMSVIDDVPVSADEDQGTGQGSEWT
                     WIEPGPLGREPASGQGAASRTGTLGNEPQTAFDELVIWK"
     gene            complement(635..>2538)
                     /locus_tag="PKN52_04630"
     CDS             complement(635..>2538)
                     /locus_tag="PKN52_04630"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNQ99261.1"
                     /translation="SNRSEQGFENRIASLVAEELGARLTYDWTAFTEDLLNLHFAEGT
                     CDVIMGVPDGFEKGLNTITYYQSPYVLAYRADADFEIDDMDDPDLAGLRLGVQGLGIP
                     PHEALRQRQLIDRVARIYGGEEGVDRLGAMLRVVESGTIDIGFGWGPVIAYYAARSQV
                     DLVVKPVEPAFDFPSIFQYIPMTMAVRRDDIAMQERLNRAIVARWDDIQAVLREYDVP
                     LIDMPKPFLGQSSLPPTTLRVGVVIPMPTRGRTLVAGVNDLVGNAARMGALQAEGIVN
                     QSGGATDRDVRVLLASSPSAEAAKRAADALLALGEVDALVGGIGDGQAEALADLADAR
                     GVPFVNIGSPSLVLREQCRPSTFHVQPSAGAYLDAMVRLYRDGPAPQRWYVVYEEGQE
                     GEALIARAEAAAQAVGDAVVGSRAITKARPVYFDLLDEAKSLGADVVAVLLDAADQVA
                     LIGQAEDGGYSLRMAPFPDPLTQTREFLAATNRYGAGRDVQRIQLWEPTLGAPAAAAM
                     NERFASRWGQPFDAPAWAAYEAVAVLTDAARRADSSDGAALAAALLEVDAGDLGGKTG
                     DVAFRQADHELRQPMYVVSINANAQWGTTLTQRLAAGRLAGTLPDGALGAAELDQLGG
                     TLGGAACGF"
BASE COUNT          379 a          868 c          925 g          366 t
ORIGIN      
        1 caggtcgttc gtgcaggcgc tcgcgcaagg gccgctgcgc ggcacgcaga cggcgaagga
       61 accggacccg gcggtgcgta gcacgctgca gcgcaaccac ttgcgcactt cgggcggctc
      121 cgtcgacaac gcggccatca tcgaggcgat ctgcgccatg gcacacaagt tgaacaagga
      181 ggtagtggcc gagggcgttg agacggccta ccagcgcgac ttcctgcgca gccttggcgc
      241 ggacaacgcg cagggctact acttcgccaa gcccatgctc ccctcgcagg cggaggaact
      301 cctgaagcgc gtcaccgtag aggccgccat ggcccaaacg gccgccgagg cacgcgcctc
      361 ggccgtgaag gagttgcggt cgtcggccgt cagggagccg tggatgccca tgtcggtcat
      421 cgacgacgtg ccggtgtccg cggacgagga tcagggcacg ggccagggca gcgagtggac
      481 ctggatcgaa ccagggccgc tcggacgaga gccggcctcg ggccaaggcg cggcgtcacg
      541 gacgggcacg cttggcaacg agccgcagac cgcgttcgac gagctcgtca tctggaagtg
      601 agcgccggtc gctcgccctg actgggagcg gctctcagaa cccgcaggcc gccccgccca
      661 acgtgccccc caactggtcg agctccgccg cgcccaaggc gccgtccggc aacgttcctg
      721 ccaggcgccc ggcggcaaga cgctgcgtga gcgtagtgcc ccactgggcg ttcgcgttga
      781 tcgacacgac gtacatgggc tggcgcagct cgtggtccgc ttggcggaaa gccacgtcgc
      841 ccgtcttgcc ccccaggtcc cccgcgtcga cttcgagcag ggccgcggcg agggcggcgc
      901 cgtccgaact gtcggcgcgg cgtgccgcgt cggtcagcac cgccaccgcc tcgtaggccg
      961 cccacgccgg ggcgtcgaag ggctggcccc aacgcgatgc gaaccgttcg ttcatcgcgg
     1021 cggcggctgg cgcgccgagc gtcggttccc agagttggat gcgctgcacg tctcggccag
     1081 cgccgtagcg gttcgtggcc gcgaggaact cgcgggtctg ggtgagcggg tccgggaagg
     1141 gggccatgcg caggctgtag ccaccgtcct ccgcctggcc gatcagcgcc acctggtcgg
     1201 cggcgtcgag cagcaccgcg accacgtcgg cgcccaggct cttggcttcg tccaacaggt
     1261 cgaagtagac ggggcgggcc ttggtgatgg ccctgctgcc gacgaccgcg tcgccgacgg
     1321 cctgcgccgc cgcctcggcg cgagcgatga gcgcctcgcc ttcctgacct tcctcataga
     1381 cgacgtacca ccgctgcggc gccggtccgt cgcggtagag ccgcaccatg gcgtcgaggt
     1441 aggcgccggc cgacggctgc acgtggaagg tcgacggcct gcactgctcg cgcagcacga
     1501 ggctcggcga gccgatgttg acgaacggca cgccgcgcgc gtcggcgagg tcggcgagcg
     1561 cctccgcctg accgtcgccg atgccgccca ccagggcgtc gacctcgccg agcgctaaca
     1621 gggcgtcggc cgcgcgcttc gccgcctccg cgctcgggct gctggccagc aggacgcgca
     1681 cgtcgcggtc ggtcgccccg ccggactggt tgacgatgcc ctccgcttgg agagcgccca
     1741 tgcgggcggc gttgccgacg aggtcgttga cgccggccac caacgttctg ccgcgggtcg
     1801 gcatggggat cacgacgccg acgcgcagcg tggtcggcgg cagggaggac tggccgagga
     1861 acggtttcgg catgtcgatc aggggtacgt cgtactcgcg cagcacggcc tggatgtcgt
     1921 cccacctggc gacgatggca cggttgaggc gctcctgcat ggcgatgtcg tcgcgccgca
     1981 ccgccatggt catggggatg tactggaaga tggacgggaa gtcgaaggcc ggttcgaccg
     2041 gcttgacgac gagatccacc tggctgcggg cggcgtagta cgcgatgaca gggccccagc
     2101 cgaagccgat gtcgatggtg ccggactcga cgaccctcaa catggcgccg agacggtcga
     2161 cgccctcctc gccgccgtag atgcgggcga cccggtcgat gagttggcgc tgccgcaacg
     2221 cctcgtgggg cgggatgccg aggccctgca cgcccagccg caggcctgcc agatccggat
     2281 cgtccatgtc gtcgatctcg aagtcggcgt cggctcggta ggcgaggacg taagggctct
     2341 ggtagtacgt gatggtgttg aggcctttct cgaagccgtc aggaacgccc atgatgacgt
     2401 cgcacgtgcc ttcggcgaag tggaggttga ggaggtcctc cgtgaaggct gtccagtcgt
     2461 aggtgaggcg cgcgccgagc tcctcggcca cgaggctggc gatgcggttc tcgaagccct
     2521 gctccgaccg gttcgaga
//
LOCUS       DAOQYE010000225         2534 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_45407_length_2534_cov_2.881807, whole genome shotgun sequence.
ACCESSION   DAOQYE010000225 DAOQYE010000000
VERSION     DAOQYE010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2534
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45407_length_2534_cov_2.881807"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1027)
                     /locus_tag="PKN52_04635"
     CDS             complement(<1..1027)
                     /locus_tag="PKN52_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99262.1"
                     /translation="MPQSVKDEATLLAFCERYDLPVRRLGTTLFGRGIHAVDLPGRAD
                     GPPILITSGSHATEPAGVLAALTLAKELRVDRRVVIVPIRDPLGWEGYANYLSLAAGT
                     TVTLTPDTDLGRLLSELGTVLYKEDDLVIAQIGEVAFLARPVAKAGRGPMDIWKHLHD
                     VLDANAGVVRTLQSRRLILPENLPDVEGCGVYDHAFTAWVTPEGGVGNFNRLFSFDDP
                     PVEVAVIADLVRELRPELVIDLHESQGSKFSIFAHPELRPGATEVYFGATGAVFAAGH
                     PIERVADIVPIIGAAHAAKFTDEGGGVFTGLNEFPGQGWQFTHLCAELGAVTVVNETG
                     RWQPLATR"
     gene            complement(1060..1881)
                     /locus_tag="PKN52_04640"
     CDS             complement(1060..1881)
                     /locus_tag="PKN52_04640"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ99263.1"
                     /translation="MPATIVLSAVVLAIIVVSALAAPLVAPHDPLAQDLINRSLLPPA
                     FSGGDWSYPLGTDALGRDLLSRIIYGGRVSLLVGLAATAIAGLLGLVIGVLSGYFGGW
                     VDIVFMRIADVQQAFPFILLAIVVMAVWGPGLLNLIIVLGVTGWVIFARVVRSIVLVL
                     KDQEFVQAAVALGTSTPRILWKHILPGVTSTMLVLATFAFVQFILAEAALSFLGLGVP
                     PPAPTWGGILSEGRVYMVMAWWITALPGVAILLTVLTVNLIGDWVRDRLDPRLRH"
     gene            complement(1934..>2534)
                     /locus_tag="PKN52_04645"
     CDS             complement(1934..>2534)
                     /locus_tag="PKN52_04645"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ99264.1"
                     /translation="LLALVVAVPLGILSAVFRNSFLDRLAIGTSLVGQSVPGFWLGLL
                     LILLFSVTLRLLPTGGSGSLKHLILPAVTLAAFTMGRIARLVRTNMLDVLGSDSLRTA
                     RAKGLREFTVVIKHGLRNAAIPIVTLIGLDASSLLGGAVITETIFAWPGLGRLVVQAI
                     SQRDFPLVQAATLCIAVIVVGVNLLVDLSYSLLDPRVNA"
BASE COUNT          445 a          787 c          955 g          347 t
ORIGIN      
        1 cgcgcgtcgc gagcggctgc cacctgcccg tctcgttcac gaccgtcacg gcgccgagct
       61 ccgcgcagag gtgggtgaac tgccaaccct ggcccgggaa ctcgttgagg ccggtgaaca
      121 cgccgccacc ctcgtccgtg aacttggcgg cgtgggccgc tccgatgatg ggaacgatgt
      181 cggcgacccg ctcgatggga tggccggcgg cgaacacggc gcccgtcgcg ccgaagtaga
      241 cctcggtcgc gccgggacgc agctccgggt gggcgaagat ggagaacttg ctcccctggc
      301 tctcgtgcag gtcgatcacc agctcgggtc gcagctcgcg cacgaggtcg gcgatcacgg
      361 cgacctcgac gggcgggtcg tcgaaagaga agagccggtt gaagttgcct acgccgcctt
      421 ccggggtcac ccaggccgtg aaggcgtggt cgtaaacgcc gcagccctcc acgtccggga
      481 ggttctccgg caggatcaga cggcgggact gcagggtcct gacgacgccg gcgttggcgt
      541 cgagcacgtc gtggaggtgc ttccagatgt ccatcggtcc gcggccggcc ttggcgaccg
      601 gccgggcgag gaacgcgacc tcgccgatct gcgcgatgac gaggtcgtcc tccttataga
      661 ggaccgtgcc gagttccgag aggagccggc cgaggtcggt gtctggcgtc agcgttacgg
      721 tcgtgccggc ggctaatgag aggtagttgg catagccttc ccagccgagc gggtcgcgga
      781 tgggcactat cactacccgc cggtcgacgc gtagctcctt ggcgagcgtg agcgcggcca
      841 ggacgccggc gggttcggtg gcgtgcgagc cggaagtgat gaggataggg ggcccgtcgg
      901 cacgacccgg caagtcgacg gcatggatgc cgcggccgaa gagcgtggtc ccgaggcggc
      961 gtactggcag gtcgtaccgc tcgcagaacg cgagcagggt ggcttcgtct ttgaccgatt
     1021 ggggcaactg ttacctccta tggtgcgagg aagcgcggtt cagtggcgaa ggcgcgggtc
     1081 gaggcggtcg cgtacccagt cgccgatcag gttgacggtg aggacggtca gcaggatcgc
     1141 caccccgggc agcgccgtga tccaccaggc catgaccatg tagacccggc cttctgagag
     1201 gatgccgccc cacgtagggg cgggcggcgg taccccgagg ccgaggaagc tcaacgcggc
     1261 ctcggcgagg atgaactgga cgaacgcgaa ggtggcgagc acgagcatcg tgctcgtgac
     1321 gccggggagg atgtgcttcc acaggatccg gggcgtcgac gtgccgaggg cgaccgcggc
     1381 ctggacgaac tcctggtcct tgaggacgag cacgatcgac ctgacgacgc gggcgaagat
     1441 cacccagccc gtcacgccca gcacgatgat gaggttgagc aggccgggac cccagacggc
     1501 catgacgacg atggcgagca ggatgaacgg gaacgcctgc tgcacgtcgg cgatacgcat
     1561 gaacacgatg tcgacccagc ccccgaagta gccggacagt acgcctatca cgagcccgag
     1621 gaggccggcg atggcggtgg cggcgaggcc gaccagcagt gacacgcgcc cgccgtagat
     1681 gatgcggctg agcaggtcgc gcccgagcgc atcggtgcca agcgggtagg accagtcgcc
     1741 gcccgagaac gccggcggga gcagcgagcg gttgatcagg tcctgggcga gtgggtcgtg
     1801 gggcgcgacg agcggcgcgg ccagcgccga gacgacgatg atcgccagga ccacggcgga
     1861 gaggacgatc gtggcaggca tgccgcgttt acgccgccga ggcttcgccc ttgcggcgct
     1921 gtcggtggcc acgttaagcg ttcacccgcg ggtcgagcag cgagtacgag aggtcgacga
     1981 gcaggttcac gccgaccacg atcaccgcga tgcagagcgt cgccgcctgt acgagcggga
     2041 agtcgcgttg gctgatggcc tgcacgacca ggcgcccgag gcctggccac gcgaagatgg
     2101 tctcggttat cacggcgccg ccaagcaggg agctggcgtc gagcccgatg agcgtcacta
     2161 tggggatggc ggcgttgcgc aggccgtgct tgattaccac cgtgaactcg cgcaagccct
     2221 tcgccctggc ggtgcgcaag gagtcgctgc cgaggacgtc gagcatgttc gtgcgaacga
     2281 gccgcgcgat gcggcccatg gtgaaggcgg cgagcgtcac cgccggcagg atcaggtgtt
     2341 tgaggctgcc gctaccgcca gtcggcagca gcctgagggt cacggagaac agcaggatga
     2401 gcaggagacc gagccagaag ccggggacgc tctgaccgac gagcgaggtg ccgatagcca
     2461 gccggtcgag gaagctgttg cggaacacgg cgctgaggat cccgagcggc accgcgacga
     2521 cgagcgccag cagc
//
LOCUS       DAOQYE010000226         2533 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_45425_length_2533_cov_3.522599, whole genome shotgun sequence.
ACCESSION   DAOQYE010000226 DAOQYE010000000
VERSION     DAOQYE010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45425_length_2533_cov_3.522599"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..671)
                     /locus_tag="PKN52_04650"
     CDS             complement(<1..671)
                     /locus_tag="PKN52_04650"
                     /inference="COORDINATES: protein motif:HMM:NF019322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNQ99265.1"
                     /translation="MRISDIRIDKALPTPAYLQLKESLGQAMDSGLLTPGSALPSERG
                     LAVSLGLSRMTVRRAFEELESDGRVEQRQGSGTYVRGQRVEQIIDSLTGFTDEVKRLG
                     LRPGTKLLSAEKVGADEATAAALGIQPGAVVLKLTRLRTANDEALALQEAHLATYLGT
                     LSLAALEHQGSLYRSLEQQFGVKPVRARQTIGARLPTRSESAALGIGREVPVLALERT
                     TYDAE"
     gene            895..>2533
                     /locus_tag="PKN52_04655"
     CDS             895..>2533
                     /locus_tag="PKN52_04655"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNQ99266.1"
                     /translation="MPRKTLITLAIVTAIAVGGAALAQPKVVSYPGLGMQPGTVGGTL
                     TLSLADSPPTFMYYGQIDNNIQTLTGVVFDSLVEFNLETYAIEPALAASWDVSEDGTV
                     YTFHLREGVKWHDGTPFTSADVVFTYTQIVANPEARAGDAAQFVYTVDGEQRAVTFAA
                     PDDNTVVITLPAPSAAFLLQQRFPIMPKHKLLQYSVEGGAAQAEINNAWSTDTPLSDV
                     VGTGPFKYDSYVAGQKVGLVRNPDYWKVDANGTSLPYAERLDYLVVRGNEAQAAQFLA
                     GNLDTLNISGAQFPDFKSREVAGADFQVITSPALFGSPPHVAFNFDDPTFGAWFSDVA
                     FRRAMEQAVDRQRIIDDVYNGLAEIPGTPTAPADGTFYEDTKSLMLPFDLAAAAAGLD
                     ALGIVDTDGNGVRNVPGGKDLEFSLTYNVDSGTFTDMATIFQNDFAKIGVKANLVGVS
                     GSALLGTGLAGDYQAIIIALGNQPDPELRKPIWQPGGSLYYWHRATQPAEPGGAPNFD
                     AMATWERRVYDIFDQGTTLVDQDARVALYKEWQRLNAV"
BASE COUNT          401 a          915 c          811 g          406 t
ORIGIN      
        1 tcctcggcgt cgtaggtcgt gcgctcgagg gccagtacgg gcacttcccg gccgatgccg
       61 agcgcggcgc tctccgagcg tgtcggcagg cgcgcgccga tggtctggcg cgcccgcacg
      121 ggcttgacgc cgaactgctg ctcgaggctt cggtagagcg agccctggtg ctcgagcgcc
      181 gcgagcgaga gcgtgccgag gtaggtggcc agatgagcct cctgcagcgc gagggcctcg
      241 tcgttcgccg tgcgcagtcg ggtcagcttc agcacgacgg cgcctggttg gatgccgagg
      301 gccgccgccg tggcctcgtc ggcccctacc ttctccgcgc tcaagagctt cgtccctggg
      361 cggaggccga ggcgcttcac ctcgtccgtg aaacccgtga ggctgtcgat gatttgctcg
      421 acgcgctggc cgcgcacgta ggtgccggag ccttgccgtt gttccacgcg cccgtcggac
      481 tccagctcct cgaaggcccg ccgcaccgtc atccgcgaga gtccgaggct caccgccaag
      541 ccccgctcgg acggtagggc gctgccgggg gtgagtaggc ccgagtccat cgcctggccc
      601 aaggactcct tcagctgcaa gtaggctggg gtaggcaggg ccttgtcgat gcggatgtcg
      661 ctgatcctca tggttcgcgt gtcgcggtcc ttccaggcgc caggcgccgg ccagggtgat
      721 tcggggcctc acattgactg tacctctgct ataccagtcg gtcaacggtc caacgccggg
      781 gagccgcgct tgggccgcgg gcgtcagtcg gggcggctgc ccttgcaggt ggtctttaac
      841 tggtctattc ttggggagta ccacggagtc tcacgcagga ttaaggaggc acgaatgccg
      901 cgcaagacgc taatcacgct tgccatcgtc acggcgatcg ccgtaggggg cgccgcgctg
      961 gcgcaaccga aggtggtcag ctacccgggc cttggcatgc agccgggcac ggtcggtgga
     1021 acgctgaccc tcagcctggc ggactcaccg ccgacgttca tgtactacgg tcagatcgac
     1081 aacaacatcc agacgctcac gggtgtcgtt ttcgactccc tggtcgagtt caacctcgag
     1141 acctacgcta tcgagccggc cctcgcggcc agctgggacg tgagcgagga cggcaccgtc
     1201 tataccttcc acctgcgcga gggcgtcaag tggcacgacg gcacgccgtt cacctcggca
     1261 gacgtcgtct tcacctacac gcagatcgtc gccaaccccg aagcccgcgc cggtgacgcg
     1321 gcccagttcg tgtacaccgt cgatggcgag cagcgcgccg tgaccttcgc ggcgcccgac
     1381 gacaacacgg tcgtcatcac gctgccggcg ccgtccgccg cgttcctcct ccagcagcgc
     1441 ttcccgatca tgccgaagca caagctgctc cagtactccg tcgagggcgg cgccgcccag
     1501 gcggagatca acaacgcctg gtcgaccgac acgccgctca gcgacgtcgt cggcaccggc
     1561 cctttcaagt acgactccta cgtggccggc cagaaggtcg gcttggtacg taaccccgac
     1621 tactggaagg tcgacgccaa cggcaccagc ctgccctacg ccgagcggct cgattacctc
     1681 gtagtgcgcg gcaacgaggc ccaggccgcc cagttcctcg cgggcaacct cgacacgctc
     1741 aacatctcgg gcgcgcagtt ccccgacttc aagagccgcg aggtggcggg cgccgacttc
     1801 caagtgatca cgagcccggc gctcttcggc tcgccgcccc acgtcgcctt caacttcgat
     1861 gacccgacgt tcggcgcgtg gttctccgac gtggccttcc gccgcgccat ggagcaggcc
     1921 gtcgaccgcc agcggatcat cgacgacgtc tacaacggcc tcgccgagat cccgggcacg
     1981 ccgaccgccc ccgccgacgg caccttctac gaggacacca agtcgttgat gctgccgttc
     2041 gacctggcag ccgccgcggc gggcctcgac gcgctcggca tcgtcgacac cgacggcaac
     2101 ggcgttcgca acgtgcccgg cggtaaggac ctcgagttct cgctgaccta caacgtcgac
     2161 tctggcacgt tcaccgacat ggccacgatc ttccagaacg acttcgcgaa gatcggtgtc
     2221 aaggcgaacc tcgtcggcgt ctccggctcc gccctcttgg gcacgggcct cgccggcgac
     2281 taccaggcga tcatcatcgc cctaggcaac cagcccgacc ccgagctccg caagcccatc
     2341 tggcagccgg gcggctcgct gtactactgg caccgcgcca cgcagccggc cgagccgggc
     2401 ggcgccccga acttcgacgc gatggccacg tgggaacgcc gcgtctacga catcttcgac
     2461 cagggcacca cgctcgtcga ccaggacgcg cgcgtcgcgc tctacaagga gtggcagcgc
     2521 ctcaacgcgg tga
//
LOCUS       DAOQYE010000227         2526 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_45639_length_2526_cov_2.894779, whole genome shotgun sequence.
ACCESSION   DAOQYE010000227 DAOQYE010000000
VERSION     DAOQYE010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45639_length_2526_cov_2.894779"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="PKN52_04660"
     CDS             complement(<1..246)
                     /locus_tag="PKN52_04660"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNQ99267.1"
                     /translation="MHGSEGSRIVLVLRDLGIGGAERVVVRLANYLAAQGVDTHLVTL
                     VGAGPLAAELTEGVSLHDLAAGRIRSAIAPLSRLLARL"
     gene            343..>2526
                     /locus_tag="PKN52_04665"
     CDS             343..>2526
                     /locus_tag="PKN52_04665"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNQ99268.1"
                     /translation="MAALHSTSRMQRGAAFLAGVSFAAVPAVGPFIAIAAIFAGRLQV
                     QRADVWWWLASVLLGAPFFFTGHPLDGALASAQIMAIWLIFRSATEFRRNARSVTVSE
                     DIGAGLVVGLVITFVVGLRQIGEFRFDLAQTVLDSIAWNVHPAVFGHAILVLSALLAI
                     VVPSPQLRVISMAIGAVGVIVSGAGEAVWAWLAIALGLQFVARRGTVGTRAAERVLIL
                     IMLLFAVGVPARLGLGTTGLLTDFAPQKEPANEFRGTELSKGDWWVPLGVSFATRDAM
                     IDGTPRTAFDVRKTSLEPWSRLQQAVTLVPGETYTLSATILAPTGARPGFDGWGREGV
                     GQTAANLGTILEAGVHRTATNGPIQVVSSSALELEGGWVRASVTFRFDGERPLTWYVG
                     VVPDRSNATGVTTSFAELQLVGSYVQLPYRPGPAARGVTDLRTSRLPIWRDALEAISA
                     RALLGWGPGGFPLAITTLHPDETLLRPVAAHAHNSFLAIWVDRGLIGLLGFVGLFALL
                     SLRAIQQRDQPAAFVLLGVLILNVFDATLLSGAVIYPLAAVLGWRAVGRREVAEAETG
                     VGSAAAVRLGLALADAVAGAAALSLGMYVAGKFDPGMSLAIGWRVPLVYATLAWPAVA
                     AAAGLYPGYGLPSYRQLSRSVRAAFMAAVSVGFVTLLLPDVFGLTASVFFVAVPAAAA
                     LAPAFRILAQKVLQRLRLWGRPVVVIGTEPATAQVARHLLAHPDIG"
BASE COUNT          343 a          918 c          822 g          443 t
ORIGIN      
        1 tagccgagcc agtaggcggg acagcggcgc gatcgcgctc ctgatcctcc cggcggcgag
       61 gtcgtggagg ctgacgcctt ccgtcaactc agcggccagc gggccggcgc ccaccaacgt
      121 caccaggtgc gtgtcgaccc cttgcgcggc gaggtagttc gccaggcgca cgaccacccg
      181 ctcggctccg ccgatgccga ggtcgcgcag cacgagaacg atccgcgaac cttcagagcc
      241 gtgcatacgc ttagtcatcc tacctaaagc cctctgccgt atagatagtc ctgcttcatc
      301 ggagcgtctc cgccgttggc cgtcacggta gactcctgac gaatggcggc cctgcatagc
      361 accagccgca tgcaacgcgg cgcggcgttc ctggctggtg tcagcttcgc ggccgtcccg
      421 gcggtcggcc ctttcatagc catagccgct atcttcgcgg gccgcctgca agtgcagcgc
      481 gcagacgttt ggtggtggct cgcgagcgtc ctcctcggag cgccgttctt cttcacaggc
      541 cacccactgg acggggcctt ggccagcgcc cagatcatgg cgatctggct catcttccgg
      601 tcggcaaccg agtttcgccg caacgcccgc agtgtcaccg tgtcggagga cataggcgca
      661 ggcctcgtcg tcggtctcgt gatcaccttc gtggtcggtc tcaggcagat cggcgagttc
      721 cgcttcgacc tggcccagac ggtcctcgac tcgatagcct ggaacgtcca cccggcggtc
      781 ttcggacacg cgatcctcgt gctctccgcg ctactggcca tcgtcgtccc gtcgccgcag
      841 ctcagggtca tctccatggc catcggcgcc gtcggcgtga tcgtctccgg tgccggcgaa
      901 gctgtatggg cctggttggc gatcgcgctc ggcctgcagt tcgtagccag gcgcggcacg
      961 gtaggaacgc gcgccgcgga gcgcgtcctg atcctgatca tgttgctctt cgcggtcgga
     1021 gtacctgcga ggctggggct cggcacgacc ggtctactca ccgacttcgc cccacagaag
     1081 gagcccgcca acgagttcag aggtaccgag ctgagcaagg gggactggtg ggtcccgctc
     1141 ggcgtgagct tcgccacgcg cgacgccatg atcgacggca ccccgaggac ggctttcgac
     1201 gtccggaaga cgtcgctgga gccgtggtcg cggctgcaac aggccgtcac cctggtaccg
     1261 ggagagacct acaccctcag cgccaccatc ctcgccccaa caggcgcgcg ccccgggttc
     1321 gacggctggg gtcgagaagg cgtcggccag acggccgcaa accttggcac catactggag
     1381 gcgggcgttc accgcactgc gacgaacggc ccgatccaag tcgtgtcctc gagcgccctg
     1441 gaactcgagg gcggttgggt tcgcgcgtcc gtgaccttcc gcttcgacgg ggagcgcccg
     1501 ctcacctggt acgtcggcgt cgtaccggac cgttccaatg ccacaggagt gaccacgtcg
     1561 ttcgcagagt tgcaactcgt cggttcgtac gtccaactgc cctaccgtcc tggtccggcg
     1621 gcgcgcggcg tcaccgacct gcgtacgtcg cggctcccca tctggcgcga cgcgctggag
     1681 gccatcagcg ctagagcgct gctgggttgg gggccgggcg ggttcccgct cgccatcacg
     1741 acgctgcacc cggacgagac gctgcttcgg ccggtcgccg cccacgctca caactcgttc
     1801 ctggccatct gggttgaccg aggcctgatc ggcctactgg gcttcgtcgg cctcttcgca
     1861 ctgctttccc tacgggccat acagcaacgc gaccaacccg ccgccttcgt tctgctcggc
     1921 gtgctcatcc tcaacgtctt cgacgccacc ctgctctccg gagccgtgat ctatccgttg
     1981 gccgccgtgc tcggctggcg cgccgtcggc cggcgggagg tggccgaggc ggagacggga
     2041 gtagggagcg ccgccgcggt caggctagga ctggcgctcg ccgacgcagt agctggagcc
     2101 gccgcgctct ctctcggcat gtacgtggcg ggcaagttcg acccgggcat gtccctggcc
     2161 atcggctggc gcgttcccct cgtctacgcc acgctcgcct ggccggccgt cgccgcggcg
     2221 gcaggcctct accccggcta cggcctcccg tcctacaggc agctcagccg ctcggtgagg
     2281 gcggcgttca tggcggccgt ctcggtcggt ttcgtcacac tgctcctgcc cgacgtgttc
     2341 ggactgaccg cctccgtgtt cttcgtggcc gtgcccgccg ccgcagcgct ggcgccggcc
     2401 ttccgcatcc tggcccagaa ggtcctccag cgcttgcggc tctggggaag gcccgtcgtg
     2461 gtcatcggca ccgaaccggc caccgcgcag gtggcgcgcc acctcctggc tcaccccgac
     2521 atcggc
//
LOCUS       DAOQYE010000228         2525 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_45659_length_2525_cov_3.525101, whole genome shotgun sequence.
ACCESSION   DAOQYE010000228 DAOQYE010000000
VERSION     DAOQYE010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2525
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45659_length_2525_cov_3.525101"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..846)
                     /locus_tag="PKN52_04670"
     CDS             complement(<1..846)
                     /locus_tag="PKN52_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99269.1"
                     /translation="MNPVRSNPMNSSGAARPAMTLVRLAAIALLLSALTTVAAQSLAA
                     LLPSETIAAVGVRGLADHQAKLQPFVDEWGRLGLTEALEDSADEGIDELGALAGVGFF
                     DLLGDEAWLAVSASNFNPLPAVTVVARVSEAGARALADMLADAASNDDSAQSFTEGSI
                     SFTLFDSDEDFPAAVALSGNFAALSTNPDTLRGVLRRYQGASEPNFTDSVAYASALAP
                     LEPFTVAFTLDYPRVADALAPLAQGMGFDQSVARVAKALRTAGAYAGASLLTDGGIES
                     TSLQVL"
     gene            complement(952..2397)
                     /locus_tag="PKN52_04675"
     CDS             complement(952..2397)
                     /locus_tag="PKN52_04675"
                     /EC_number="2.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016744 - transketolase or transaldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HNQ99270.1"
                     /translation="MPARYRAYGWHVQTVSDGNDVLALRQALSAARTETARPSLIAVR
                     TVIGYGSPKLAGTSKVHGSVLGAEEAAATKANLGIDWPEFTVPDDVFHHMRSAVKRGA
                     EARATWQELFERYRAAHPELAAEFERVMREKLPAGFDADLPTYPVGKSVATRKASLEA
                     INALARRLPELVGGSADLAGSNFTDIEGETAMRAGDYAGRIIHFGIREHAMAAIGNGL
                     SLHGGLRPFVSTFLIFSDYLRPALRLSALMGQGVVYDFTHDSIGLGGDGPTHQPIGEL
                     AALRAMPGLDVIRPADANEAVQAWVSALRNQTRPTALAFTRQNVPNLAVPAGAVARGA
                     YVLAEAGAGGGLAEGGAGATLTPDPDARPDLILIGTGSEVQLCLAARDVLEAEGVRTR
                     VVSMPSTEIFARQDDAYRAAVLPAAVRARVAVEAGATFGWERYTGELGEIIGVDRFGA
                     SAPGDVLMREYGFTTENVVAGARRALGRAKA"
BASE COUNT          346 a          869 c          946 g          364 t
ORIGIN      
        1 gagcacctgc agggaggtcg actcgatgcc gccgtccgtc aggagcgagg cgccggcgta
       61 ggcgccggcc gtgcgcaggg ccttggcaac ccgcgccacg ctctggtcga agcccatgcc
      121 ctgcgcgagc ggggcgaggg cgtccgccac gcgcgggtag tcgagggtga aggcgaccgt
      181 gaacggctcg agcggggcga gagcggaggc gtacgccacc gaatcggtga agttcggctc
      241 gctcgcgcct tggtagcgac gcagcacgcc gcgaagggtg tccgggttgg tggatagcgc
      301 tgcgaagttg cccgagaggg cgacggccgc cgggaagtcc tcatccgagt cgaacagcgt
      361 gaacgagatg ctgccctcgg tgaaactctg ggcggagtcg tcgttgctgg cggcatcggc
      421 cagcatgtcg gcgagcgccc gcgcgccggc ctcggagacc cgcgcgacca ccgtgaccgc
      481 cgggagcggg ttgaagttgg aagccgacac ggccagccag gcctcgtcgc ccagcaggtc
      541 gaagaagccg acgcccgcga gggcgccaag ctcgtcgatg ccctcgtcgg ccgagtcctc
      601 gagcgcttcc gtgaggccga ggcgccccca ctcgtcgacg aacggttgga gcttcgcctg
      661 atggtcggcc agccccctca cccccacggc ggcgatggtc tcggagggga gcagcgccgc
      721 gagcgactgg gcggcgacgg tggtcaaagc gctcagcagc agggcgatcg ccgcgaggcg
      781 tactagcgtc atggcggggc gggccgcccc cgagctgttc attgggttgg accggactgg
      841 gttcatgatc ctcctgcgtg atgcacggct caaggttacg gtagcccggc cgcgcggcgt
      901 gtacgggtgc ccacttctgg ccgggcgccg gaggggtttc gcccggagcc cctaggcctt
      961 ggcccgtccc agcgccctgc gggcgccggc cacgacgttc tcggtcgtga agccgtactc
     1021 gcgcatgagc acatcgcccg gcgccgaggc gccgaagcgg tctaccccga tgatctcgcc
     1081 cagctcgccc gtgtagcgct cccagccgaa ggtcgcgccg gcctcgacgg ccacgcgcgc
     1141 tcgcaccgcc gccggcagca cggccgcgcg gtaggcgtcg tcctgtctgg cgaagatctc
     1201 ggtgctcggc atgctcacga cgcgggttct caccccttcg gcctcgagca cgtcgcgggc
     1261 ggccaggcag agctgcacct ccgagcccgt gccgatgagg atcaggtcgg gcctcgcgtc
     1321 cgggtccggc gttagcgtcg cgcctgcgcc gccctccgcc aggccgccgc ccgcgccggc
     1381 ctcggccagt acgtaggcgc cgcgtgccac cgcgccggcc ggcacggcca ggttgggcac
     1441 gttctggcgc gtgaacgcca gggctgtcgg cctagtctgg ttgcgcagcg cgctgaccca
     1501 cgcctgcacc gcctcgttgg cgtcggccgg cctgatcacg tcgagaccgg gcatcgccct
     1561 cagcgcggcc aactcgccga tcggttggtg cgttggcccg tctccgccca gtccgatgct
     1621 gtcgtgggtg aagtcgtaga cgacgccctg gcccatgagc gccgagaggc gcagggcggg
     1681 acgcaggtag tccgagaaga tcaggaacgt ggagacgaac gggcgcagcc cgccgtggag
     1741 gctgaggccg ttgccgatgg ccgccatggc atgctcgcgg atgccgaaat ggatgatgcg
     1801 accggcgtag tcgccggcgc gcatggccgt ctcgccttcg atgtccgtga agttcgagcc
     1861 ggccagatcg gcggagccgc ccacgagttc tggcaggcgc ctggcgagcg cgttgatggc
     1921 ctccagcgac gctttgcgcg tggcgacgct cttgccgacc gggtaggtcg gcaggtcggc
     1981 gtcaaaaccc gccggcagct tctcgcgcat gacgcgctcg aactcggccg ccaactcggg
     2041 gtgggccgcg cggtagcgct cgaagagctc ctgccaggtc gcgcgggcct cggcgccgcg
     2101 cttgacggcg gagcgcatgt ggtgaaagac gtcgtccggc acggtgaact ccggccagtc
     2161 gatgcccagg ttggccttcg ttgccgccgc ctcctcggcg ccgagcacgg agccgtggac
     2221 cttggaggta ccggccagct tgggtgatcc gtagccgatg acggtgcgca ccgcgatgag
     2281 gctgggcctg gccgtctcgg tgcgcgcggc ggagagggcc tgcctgagcg ccagcacgtc
     2341 gttgccgtcg gagaccgtct gcacgtgcca gccgtaggcg cggtaacgcg ccggcacgtc
     2401 ctccgtgaag gtgatgctcg tcgggccgtc gatggagatg ccggcgcgtt accgcgccta
     2461 cggctggcac gatgagcttg ccgaggccga ggtgtccggc gaacgacgag gcctcggcgg
     2521 cgacg
//
LOCUS       DAOQYE010000229         2524 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_45696_length_2524_cov_2.634670, whole genome shotgun sequence.
ACCESSION   DAOQYE010000229 DAOQYE010000000
VERSION     DAOQYE010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45696_length_2524_cov_2.634670"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /locus_tag="PKN52_04680"
     CDS             <1..490
                     /locus_tag="PKN52_04680"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HNQ99271.1"
                     /translation="AIGQDWHPEDATTERTLLAQEVLFPFTDEQRTRLVTEVIELLGD
                     YWRLVGSALPALGGRDVDRAELPLAVRYGETVWEGVLDRLYRVGGEWYLDDYKTDRRV
                     APERYYLQLAHYARAAQRALGVRPRSRLVYLRSGRVIEPAEDLLEEAFAGSPIADALR
                     SA"
     gene            complement(581..1252)
                     /locus_tag="PKN52_04685"
     CDS             complement(581..1252)
                     /locus_tag="PKN52_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99272.1"
                     /translation="MRGIGSLNETPLHAELKRRVAPPGSAFEVEVGGYVIDAVSDGLL
                     IEVQTRNLGGMRTKLGALLPENRVRLVLPVPRTRWLEKAHPDGRIERRRSPKTGRVEQ
                     LFAELVYAPTVLAHPNLELEVVLVEDVEERAFATGKAWRRRGWVVSGRRLVAVLERRT
                     FERPEDLLALLPAELPHEFTTAELAGAARLPLRLAQQAAYCLHALELTERVGKRGRAH
                     LYRLA"
     gene            1351..1611
                     /locus_tag="PKN52_04690"
     CDS             1351..1611
                     /locus_tag="PKN52_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99273.1"
                     /translation="MEAKHGEATVAGRRGMLVTDERVGLVWEAAVTVDEFRGLVEFCG
                     LGNLLEVAGVSGAFKAMARRWWVLPMGDEMLVRIGLERVAAA"
     gene            complement(1667..>2524)
                     /locus_tag="PKN52_04695"
     CDS             complement(1667..>2524)
                     /locus_tag="PKN52_04695"
                     /inference="COORDINATES: protein motif:HMM:NF024748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HNQ99274.1"
                     /translation="EDGAGGAAPTPGGNHATPAALSVVAFPLYGAPGEGFISYRAAEQ
                     FTAASTIKVYVLQALMERVAAGDADLDDELPVTAQDQVSGSGVLKALTPGRRYTLRDL
                     ATLMIVVSDNTATNVLIEYVGVEALNASIQAHGWVGTRSAGKLQLAPVDGGPRRSPSV
                     TTAADLADYFRGLWLGELLPAPLTEEAKAIYRKQQYCEMGRSIDYDSYDASIGEAPWL
                     IASKSGSVRGVRNDAGVFEPLTAAASGAKPFVVAIMTKGCVDERFHSDNLGARVVGWA
                     AAEVVKRLA"
BASE COUNT          353 a          847 c          929 g          395 t
ORIGIN      
        1 cgccatcggt caggactggc atcctgagga cgcgaccacc gagcgcacgc tgctcgccca
       61 ggaagtcctc ttcccgttca ccgacgagca gcgcacgcgc ctcgtgaccg aggtcatcga
      121 gctgctcggc gactattggc gcctggtggg ctcggcgctg cccgccctcg gcgggcgcga
      181 cgtcgaccgc gccgagctgc ccttggcagt gcgctacggg gagaccgtct gggagggcgt
      241 cctcgaccgc ctgtaccggg tcggcggcga gtggtacctg gacgactaca agaccgaccg
      301 ccgagtggcg cccgagcggt actacctgca gctggcccat tacgcgaggg cggcgcagcg
      361 cgcgctcggc gtgcggcctc gctcgcgcct cgtctaccta cggagcggcc gggtcatcga
      421 gcccgccgag gacctcctgg aagaggcgtt cgccggctca cccatcgctg acgccttgcg
      481 gtcggcatga tcccgcgtgg actggcgtgg accggcgttg atctgcgccc acgggcgacc
      541 accgacgccg gtccgtgccg cgggtgcggt atggcccatg tcaggcgaga cggtacaggt
      601 gggcgcggcc ccgcttgccg acgcgttcgg tcaactcgag cgcatgtaag cagtacgccg
      661 cctgttgtgc gaggcgcaac gggagcctcg cggcgccggc cagttccgcc gtggtgaact
      721 cgtggggcag ctctgcgggg agcagggcca acaggtcctc gggccgctcg aacgtcctgc
      781 gttccaggac cgcgacaagg cgtctgccgc tcaccaccca gccgcgcctg cgccaagcct
      841 tcccggtcgc gaaggcgcgc tcctccacgt cctcgacgag gactacctcc agctccaggt
      901 tgggatgggc cagtacggtc ggagcgtaga ccagttccgc gaagagttgc tcgacccgcc
      961 cggtcttcgg ggagcgacgg cgctcgatgc gcccgtcggg atgcgctttc tctagccagc
     1021 gggtgcgggg aacgggcagc accagccgca cccggttctc gggcagcagg gcgccaagct
     1081 tggtacgcat gccgccaagg ttgcgcgtct ggacttcgat gaggagcccg tcggacaccg
     1141 cgtcgatgac gtacccgccg acctcgacct cgaaagcgct tcccggtggc gcgacccggc
     1201 gcttgagctc ggcgtgcaga ggcgtctcgt tgagggagcc tatgccgcgc atggttgcac
     1261 gatacccaag cctaggccgg ggcctaggca aggtaggcag tagtcccggt aggcgccccg
     1321 gcggcggggg tacgcgtgga tgctgggcga atggaagcta aacatggtga ggccacggtg
     1381 gcaggtaggc gcggcatgct agtgaccgac gagcgcgtcg gcttggtctg ggaggccgcg
     1441 gtaacggtcg acgagttcag aggcctcgtc gagttctgcg ggctcggcaa cctgctcgag
     1501 gtggccggcg tctccggcgc cttcaaggcc atggcccgta ggtggtgggt gttgccgatg
     1561 ggcgacgaga tgctcgtgcg catcgggctc gagcgggtgg cggcggcatg agcgctacgc
     1621 aaggcgtttg accacgtccg ccgccgccgg ttcgtgcgcg cgggcgtcac gccaggcgtt
     1681 taaccacttc cgccgccgcc catccgacca cgcgggcgcc gaggttgtcg gagtggaagc
     1741 gctcgtcgac gcaccccttg gtcatgatgg cgacgacgaa gggcttggcg ccgctggcgg
     1801 cggccgtcag gggctcgaac acgccggcgt cgttgcggac cccacgcacc gagccgctct
     1861 tggaggcgat gagccacggt gcctcgccga tggaggcgtc gtaggagtcg tagtcgatgc
     1921 tcctgcccat ctcgcagtac tgctgtttgc ggtagatggc cttggcctcc tctgttaggg
     1981 gggccggcag gagttcaccg agccagagcc cgcggaagta gtcggccagg tccgccgcgg
     2041 tggtcacgga cggcgagcgt cgcgggccgc cgtcgacggg ggcgagctgc aacttcccgg
     2101 cgctgcgggt tccgacccag ccgtgcgcct ggatggaggc gttgagcgcc tcgacgccga
     2161 cgtactcgat caggacgttg gtggccgtgt tgtcgctgac gacgatcatc agggtcgcga
     2221 ggtccctcag cgtgtagcgc cgccccggcg tcagggcctt caggacgccg gagccggaca
     2281 cctggtcctg ggcggtcacc ggcagttcgt cgtccaggtc ggcgtcaccg gccgccacgc
     2341 gctccatgag ggcctgcaga acgtagacct tgatggtgct ggcggccgtg aactgctcgg
     2401 cggcacggta gctgatgaag ccttcgccgg gtgcgccgta gagcgggaac gccaccaccg
     2461 agagggccgc cggcgtcgca tggttcccac ccggggtggg cgccgcgccg cctgcgccgt
     2521 cttc
//
LOCUS       DAOQYE010000230         2521 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_45775_length_2521_cov_3.414436, whole genome shotgun sequence.
ACCESSION   DAOQYE010000230 DAOQYE010000000
VERSION     DAOQYE010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2521
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45775_length_2521_cov_3.414436"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            39..425
                     /locus_tag="PKN52_04700"
     CDS             39..425
                     /locus_tag="PKN52_04700"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HNQ99275.1"
                     /translation="MIAAVTALGFNLGNLAIIAGALSVGIGFGLQSIVNNFVSGLILL
                     FERPIKVGDWVVTSSGEGTVKKISVRSTEKPPTTAVKVPTSSPSSPSRQLPLTWVGAV
                     YWPSTRSARPGTCDSNGMRLKRASMV"
     gene            complement(709..1050)
                     /gene="yidD"
                     /locus_tag="PKN52_04705"
     CDS             complement(709..1050)
                     /gene="yidD"
                     /locus_tag="PKN52_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HNQ99276.1"
                     /translation="MSVPTRSDGGRSLGRVAKDVAIWPLKAYQRYLSPLKGAPSCRFH
                     PTCSAYAIEAIERHGVLRGSWLGLKRVLRCHPFHPGGYDPVPPKPRQRHHEPEHQALD
                     RTDVHVHTEAP"
     gene            complement(1047..1385)
                     /gene="rnpA"
                     /locus_tag="PKN52_04710"
     CDS             complement(1047..1385)
                     /gene="rnpA"
                     /locus_tag="PKN52_04710"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HNQ99277.1"
                     /translation="MSGDRAFQRLRKGSTGGGKHVTVRWRPTRSGEVRVGIVVSRKVG
                     KAVVRNRVRRRLREGVRELVGQPLREQAYAASMPSFDLLVIARPSAATTDYHELKRSL
                     ASAFERAGLR"
     gene            complement(1409..1552)
                     /gene="rpmH"
                     /locus_tag="PKN52_04715"
     CDS             complement(1409..1552)
                     /gene="rpmH"
                     /locus_tag="PKN52_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HNQ99278.1"
                     /translation="MKRTYQPNRRKRATTHGFRARMATPGGRNVIKRRRAKGRKRLSV
                     SDR"
     gene            complement(1765..>2521)
                     /locus_tag="PKN52_04720"
     CDS             complement(1765..>2521)
                     /locus_tag="PKN52_04720"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HNQ99279.1"
                     /translation="TSFDLDPGAQTAAPPGDAVGGGLAGARFGIVKELSGEGFGPDAL
                     ASLAKARAALEAAGADVVEVSLPGTALAVACYYVIAAAEASSNLARYSGMLYGQRVGE
                     EREGQELVMRATRGALFGKEVKRRILLGSFALSAGYYDAYYGQALKVRQRITREMDAA
                     LAGVNALVTPTAPGPAYALNEKTADPLSMYVGDVATCLANLSGGPAVSVPCGTAEAEG
                     RGSGLPLGVQLVGRAGSDGALLRLAALQEAALA"
BASE COUNT          377 a          869 c          867 g          408 t
ORIGIN      
        1 tgacgacgct gatcggttac gccgggctta tcgtcgcgat gattgccgcc gtgaccgcgc
       61 tcggcttcaa tctcggcaac ttggcgatca tcgccggcgc cttgtcggtc ggcatcggtt
      121 tcggcctgca gtcgatcgtc aacaatttcg tttcgggact catccttctg ttcgagcggc
      181 cgataaaggt cggggactgg gtggtgacga gttcgggcga gggcacggtg aaaaaaatca
      241 gcgtgcgctc gacggagaaa ccgcccacca ccgccgtgaa ggtgccgacg agcagcccct
      301 cgtcgccctc ccgccagctg cccctgacct gggtcggcgc cgtgtactgg ccgtcgacga
      361 ggagcgcgag acctgggacc tgcgactcga acgggatgag gttgaagcgc gcatcgatgg
      421 tgtagtcacc catgcgctgg ccgcgctgct gcttgccgta ggcggccacg agctgcccgg
      481 agccgtcgaa gatgaaatcg acgcccttgc tcgtgatgag cgtaaggtct tgcccatcct
      541 cgcagaagaa ggacggcgtc tgggtggagc aggtcgcggt tatcttctcg gcgccgcgga
      601 agtcggtctc acggaaggcc gtggcagcca gctcgacgcg cgcgttcgcg aacgtcgcca
      661 gggagagcgc cgccatgagc gctaccagct gcagtcttgt gacgcgtttc aaggggcctc
      721 cgtgtggaca tggacgtcgg tgcgatccaa cgcctgatgt tcaggttcgt ggtgccgctg
      781 ccgcggcttg ggcggcactg ggtcgtagcc gcccgggtgg aacgggtggc accgcaagac
      841 acgcttcaac cccaaccagc tgccccgcaa aacgccgtgc cgctcgatgg cttcgatggc
      901 gtaggcgctg caggtggggt ggaaccggca cgacggcgcg cccttgagtg gagagaggta
      961 gcgctggtag gccttcagcg gccagatggc cacgtccttg gcgacacgcc cgagcgagcg
     1021 gccgccatcg ctgcgagtcg gcacgctcat cgcagtcctg cgcgttcgaa cgcgctcgcg
     1081 agggaccgct tcaactcgtg atagtcggtc gtggcggccg acggcctggc gatgacgagg
     1141 aggtcgaacg acggcatgga ggcggcgtag gcctgctcgc gcaacggctg tccgacgagc
     1201 tcccggacgc cctcgcgcag ccggcgtcgg acgcggttac gcaccacggc cttgccgacc
     1261 ttccggctca ccacgatgcc cactctgacc tccccggagc gcgtcggtcg ccaacgcacg
     1321 gtcacgtgct tgccgccgcc ggtgctccct ttgcgcagtc gttggaacgc gcggtcgccg
     1381 ctcaaggagc ggagcaccgc tactcgtctc agcgatccga tacggacagg cgcttacgtc
     1441 ccttggcacg gcgccgcttg atgacgttgc ggccacctgg tgtcgccatg cgcgcgcgga
     1501 acccgtgcgt cgtggcacgc ttcctgcggt tgggctgata cgtccgcttc atggtgtctc
     1561 cttccagtct cttaccgtcg ggtggatgtg cccgcggcac gctgcgagct ttagggcgcg
     1621 cggcgcttcg tctactcctc gcgaccctta agcgagctgc cgtccgggca ggacagtcaa
     1681 gccgtggaat agtatcacac cgccggcctt ccaggcacca ggcaccggct gcgaaggggg
     1741 cgtcgatccg cgctttgacg tcgctcaggc cagagcggct tcctgcaggg cggcgaggcg
     1801 cagcaacgcc ccgtccgacc cggcgcgacc gacgagctgc acgcccaacg gcaagcccga
     1861 ccctcgccct tcggcttcgg ccgtgccgca cggcacgctc acggccggcc cgcccgagag
     1921 gttggcgagg cacgtggcca cgtcgccgac gtacatggag agagggtcgg ccgtcttctc
     1981 gttcaaggcg tacgctgggc cgggcgcggt cggggtgaca agcgcgttca cgcccgccag
     2041 cgcggcgtcc atctcgcgcg ttatgcgctg acgcaccttc agggcctggc cgtagtaggc
     2101 gtcgtagtag ccggcggaca gagcgaacga ccccaggagg atcctgcgct tcacctcctt
     2161 gccgaacagc gcgccgcgcg tggcccgcat gaccagttcc tgaccctcgc gctcctcgcc
     2221 tacccgctgg ccgtagagca tgccggagta acgcgcgagg ttgctcgacg cctccgccgc
     2281 cgcgatcacg tagtagcagg ccaccgcgag ggcggtgccc ggcagcgaga cctccacgac
     2341 gtcggccccc gcggcctcga gggcggcgcg ggccttcgcc agtgacgcga gcgcgtcggg
     2401 gccgaacccc tcgccggaga gctccttgac gattccgaaa cgcgccccgg ccagcccgcc
     2461 gcccaccgcg tcgccgggcg gcgcagcggt ctgggcgcct gggtccagat cgaagctcgt
     2521 g
//
LOCUS       DAOQYE010000231         2520 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_45806_length_2520_cov_3.098174, whole genome shotgun sequence.
ACCESSION   DAOQYE010000231 DAOQYE010000000
VERSION     DAOQYE010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45806_length_2520_cov_3.098174"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="PKN52_04725"
     CDS             <1..454
                     /locus_tag="PKN52_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99280.1"
                     /translation="EFQRLDYAPQMLASVTLLDPALMANPAMQGALFSVFLRLPSVIV
                     GEGNGDPIADQLYRDVIVAYAPEIARDPFRALAGIAFAEPAVLALQAAGRDLTRESFK
                     EALHSIDGYTTGLYNNLSFKDGSQGNNSVMLLQVTPQGLRPVSDWLSL"
     gene            509..1315
                     /locus_tag="PKN52_04730"
     CDS             509..1315
                     /locus_tag="PKN52_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ99281.1"
                     /translation="MTDHLLALENVSLSFRGLTALSDVSLGIAPGEVVALIGPNGAGK
                     TSLFNVICGFYRPRSGKVMWRGTDITGNAPHMVARLGIGRSFQNIELFKTLTCLDNLL
                     LGRHLHVKSNVFSAMLSTRAWVRDEVAQRRRAEELMDLLDLQAYRDQRVGALPYGVQK
                     LIEVARALAAEPTLLLLDEPAAGMTVEEKDDMMVTLLKLRRELDLTMLVVEHDLRVVS
                     RLADRVVVLDHGVKIADGSPSSVQDDPAVVRAYLGGTRLAPESAGAEAQR"
     gene            1312..2112
                     /locus_tag="PKN52_04735"
     CDS             1312..2112
                     /locus_tag="PKN52_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ99282.1"
                     /translation="MSDHPTTGAALLELRTVESGYHKQLRVLKGVSLSILPGTCVAVL
                     GSNGAGKSTMLKTIMGLVEDEPRKGTVWALGKNVTRADTERISQAGIAYVVEDRGMFP
                     DLTVAESLRLGAFHRRDRSAIAADLERMFTYFPRLAERLKQDSGTLSGGEQQMLAIAR
                     ALMSRPKLLMLDEPSLGLAPMLVEEIFRVIREINREGMGILLVEQNANQALAIADYGY
                     VLEGGRFVLEGTAAELRANENVQELYLGVRTGGDEPRLHVRRRRRRWN"
     gene            complement(2305..>2520)
                     /locus_tag="PKN52_04740"
     CDS             complement(2305..>2520)
                     /locus_tag="PKN52_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HNQ99283.1"
                     /translation="KVKWFSSEKGFGFIEQGGGQPDVFVHFSAIQGAGFRNLNEGDEV
                     SFDVEQGKKGLQAANVVVTASTAGGRY"
BASE COUNT          410 a          887 c          832 g          391 t
ORIGIN      
        1 cgagttccag cgcctcgact acgccccgca gatgctcgcg tccgtcacac tcctcgaccc
       61 ggcgctcatg gccaaccccg ccatgcaggg cgcgctcttc tccgtgttcc tgaggttgcc
      121 gagcgtcatc gtcggcgagg gcaacggcga cccgatcgca gaccagctct accgcgacgt
      181 catcgtggcc tacgcccccg agatcgcccg cgacccgttc cgggcgctcg ccggcatcgc
      241 gttcgccgaa cccgccgtgt tggcgctcca ggcggccggc cgcgacctga cgcgcgagtc
      301 cttcaaggaa gctctgcaca gcatcgacgg gtacacgacc ggcctctaca acaacctgtc
      361 gttcaaggac ggcagccagg ggaacaactc cgtgatgctc ctgcaggtga ccccgcaggg
      421 cctgcggccc gtctccgact ggctctcgct gtaacagaac cgccaggtgt ggggcgctat
      481 tgggcgcccc acaccccatc tcatcgacat gaccgaccat ctgttggccc tcgagaacgt
      541 ctcgctctcc ttccgggggc tcaccgcgct gtcagacgtc tcgctgggga tagcccccgg
      601 cgaggtggtt gccctcatcg gtccgaacgg cgcgggtaag accagtctct tcaacgtcat
      661 atgcggcttc taccggccgc gctcaggcaa ggtcatgtgg cgcggcaccg acatcacggg
      721 gaacgcgccc cacatggtcg cccgcctcgg catcgggcgc tcttttcaga acatcgagct
      781 gttcaagacc ctcacctgcc tcgacaacct cctcctcgga cggcacctgc acgtgaagtc
      841 caacgtgttc tccgccatgc tctccactcg cgcctgggtg cgcgacgagg tcgcccaacg
      901 gcgccgcgcc gaggagctga tggacctcct cgacctgcag gcgtaccgcg accagcgcgt
      961 tggcgccctg ccgtacgggg tccagaagct catcgaggtc gcgcgcgcgc tggcggccga
     1021 acccacgctc ctgctactcg atgagcctgc cgccggcatg acggtcgagg agaaggacga
     1081 catgatggtc accctcctca agctcaggcg cgagctcgac ctcacgatgt tggtcgtgga
     1141 gcacgacctg cgcgtcgtca gccggctggc cgaccgcgtc gtggtcctcg accacggggt
     1201 gaagatcgcc gacggcagcc cgagctccgt ccaggacgac ccggccgtgg tgcgcgccta
     1261 cctcggcggc acgcgcctgg cgcccgagtc ggccggcgcg gaggcgcagc gatgagcgac
     1321 catcccacga ccggcgcggc gctgctggaa ctgcgcaccg tcgagagcgg ctaccacaag
     1381 cagctgcgcg tgctcaaggg cgtctcgctc agcatcctgc cgggcacgtg cgtggcagtg
     1441 ctcggctcca acggcgccgg caagagcacc atgctcaaga cgatcatggg cctggtcgag
     1501 gacgagccgc gcaagggcac cgtctgggcg ctgggcaaga acgtgacgcg cgccgacacc
     1561 gagcgcatct cgcaggccgg gatcgcgtac gtggtcgagg accggggcat gttccccgac
     1621 ctgacggtgg ccgagagcct tcggctcggc gccttccata ggcgcgaccg gtccgcgatc
     1681 gcggccgacc tcgagcgcat gttcacgtac ttcccgcgct tggcggagcg cctcaagcag
     1741 gactccggaa cgctgtctgg cggggagcag cagatgctcg ccatcgcgag ggcgctcatg
     1801 agccgcccga agctcctgat gctcgacgag ccgagccttg ggctcgcgcc catgctcgtc
     1861 gaggagatct tcagggtcat ccgcgagatc aaccgcgagg ggatgggcat cctcctggtc
     1921 gagcagaacg ccaaccaggc gctggcgatc gcggattacg gctacgtgtt ggaaggcggc
     1981 cgcttcgtgc tcgagggcac ggcggcggaa ctgcgcgcca acgagaacgt gcaggagctc
     2041 tatctgggcg tgcgcaccgg cggcgacgaa ccccgcctgc acgtgcgcag gcgccgcagg
     2101 cgctggaact gagcggaagg cgcatacggc ggcgtcagga gggctgcgcc tggtcggacg
     2161 ccgccagtgt ggcccgtgga cgacctcggt tggttcgggg acgacaaacc aaccgtggat
     2221 gacgaaccaa aagacggcgc ccgtggggcc gtccttgcgg acggggttcc acgggcgccg
     2281 ttcttcgact agtgagctgt cagctcagta gcgaccgccg gcagtcgagg ccgtgacgac
     2341 cacgttggcc gcctgaaggc ccttcttgcc ctgctcgacg tcgaaggaga cttcgtcgcc
     2401 ttcgttgagg ttgcggaagc ccgcaccctg gatggcggag aagtgaacga agacgtccgg
     2461 ctggccgccg ccttgttcga tgaagccgaa gcccttctcg ctgctgaacc acttgacctt
//
LOCUS       DAOQYE010000232         2518 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_45867_length_2518_cov_2.607389, whole genome shotgun sequence.
ACCESSION   DAOQYE010000232 DAOQYE010000000
VERSION     DAOQYE010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2518
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45867_length_2518_cov_2.607389"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            195..848
                     /locus_tag="PKN52_04745"
     CDS             195..848
                     /locus_tag="PKN52_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNQ99284.1"
                     /translation="MVVLDRQAARFIVDPSASRFLEPFIGRSRTASSVAEELGVKVSS
                     VLYRIRQLTDLRLVEVERVERRRGRPMKHYRATAEAFFVPFEVTDAESVEALGTMSAA
                     GARHHLAESLSAAWADAARCHPDWGVRLGRDGDGNLDRSIVPEALSKGMESFRESLLG
                     YDSPPLWDQHFLLQLSLSEAKTLQRELSDLYGRYYRPDGQGRKPYILRLAMAPLKAG"
     gene            1160..1312
                     /locus_tag="PKN52_04750"
     CDS             1160..1312
                     /locus_tag="PKN52_04750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99285.1"
                     /translation="MQLVKMSIERRDQALALLAMGGAPEGRDTSSQFITSFVPLKNVD
                     RIVEYS"
     gene            1344..1745
                     /locus_tag="PKN52_04755"
     CDS             1344..1745
                     /locus_tag="PKN52_04755"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNQ99286.1"
                     /translation="MAVTFHVSAAVDDAALSRLHHRAFGSASTAVVPWSERLSRYSLF
                     WVSATEAEDIVGFVNVIGDGGAHAVVLDTVVSPEMQGRGIGKQLIEIAATEARARGCH
                     WLHVDFEPRLAGFYVDVCGFRATTSGLRRLI"
     gene            1818..2468
                     /locus_tag="PKN52_04760"
     CDS             1818..2468
                     /locus_tag="PKN52_04760"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNQ99287.1"
                     /translation="MSTTERKPRTDTTTTRDATVTVLPANEASWDDLQTVFREGGDAA
                     GCQCQWFKLQRAEWRSTPRAERTALLRQQTACGHAEATHTTGLVAYLDGEPAGWCAVE
                     PRTAYPRLLTARVPWAGRDEVRSDETVWAITCFVTRQGFRSKGVSRALARAAVEFARS
                     RGASAVEGYARRTNPGAKQSPGPLFVGTPSIFAAAGLREVSQPTPGRVVMRIDFGG"
BASE COUNT          447 a          828 c          824 g          419 t
ORIGIN      
        1 agatggcctg accaaaggag aagatcagga aagcggacca gccgcgaaag gccttccttc
       61 cagtcattga cgtgtggtct tcgtcgggtg gcctctccac cgcccacacc gaacgccgat
      121 cgaggaagtc atcgccgcaa agcttgcgaa gtattcttgt aagaatggaa tgggctcttc
      181 taagtagaga aaccttggtc gttctggacc ggcaagccgc ccggttcatc gtcgacccga
      241 gcgcctcgcg tttcctcgag ccgttcatcg ggcgcagccg gacggcgagc tcggtggcag
      301 aggaactcgg tgtcaaggtc agttctgtcc tctaccggat ccgccagctc accgacctcc
      361 gcctcgtcga ggtcgaacgt gtggaacggc ggcgaggacg ccccatgaag cattaccgcg
      421 ccacggcgga agccttcttt gtgcctttcg aggtcacgga cgccgagtcc gtcgaggcgc
      481 tcggcaccat gtcagccgca ggcgctcggc accatctagc ggagagcctg agcgccgcgt
      541 gggcagacgc cgctcgatgt cacccggact ggggcgtgcg gctcggacgc gacggtgacg
      601 gcaacctgga taggtccata gttccagaag cgttatccaa gggcatggag agcttccgcg
      661 agtccctgct cggttacgac tcccctcccc tttgggacca gcacttccta ctgcagctca
      721 gtctctccga ggcaaaaacg cttcagagag aactcagtga cttatacggc cggtactacc
      781 gacccgacgg gcagggcagg aagccgtaca tcttgcggct cgccatggct ccgctcaagg
      841 cgggttaagt ccattcgccg cccccgcagt gcactccccc gcgcatcgcg tgtccgattg
      901 gcgcaggctt caggaactcg atcgtctggg ccaccgtagc aatcatctgt cgactgatca
      961 tgatcgcgcg gggcgtgcga gcgcgtaacg ccctgaggca cgtgctcgtg gcggggctcg
     1021 gccggtttgg tgcgaaaccg tctaccattc gtacctggca gacctggatg gcggcccgac
     1081 cggcacctag ccaacgcccc tacggccggc ttcaacgcca atcgtaccgc gctgaagccg
     1141 gttcgtccga tactccggca tgcaactagt gaagatgagc atcgagcgcc gcgaccaagc
     1201 gctggccctg ctggcgatgg gcggggcacc ggaaggcagg gacacgagca gccagttcat
     1261 aacgagtttc gtaccgctca agaacgtcga ccgcatagtc gaatacagtt gaactagaga
     1321 gtcatgagtc ggaggcaccg cgcatagctg tcacttttca cgtatccgcc gccgtcgatg
     1381 acgctgccct atcgcgctta caccatcgtg ccttcggctc ggcttctacg gctgtcgtgc
     1441 catggagcga gcgcctaagc cgctacagcc tcttctgggt cagcgcgacc gaggccgagg
     1501 acatcgtcgg gttcgtgaac gtcataggcg acgggggtgc gcacgccgtc gtcctcgaca
     1561 ccgtcgtgtc gcccgagatg cagggcaggg ggatcggcaa acagctgatc gagatagcgg
     1621 caaccgaggc ccgcgcccgc gggtgtcact ggctgcacgt cgatttcgag ccgcgactag
     1681 cgggcttcta tgtggacgtc tgtgggttcc gggcgacgac gtcagggctt cgaaggttga
     1741 tctgagagcg gcacactcac tacagctggg agtgccatcc gcagggcccg gcgtcgtaag
     1801 tggtgctagg ttgtgccatg tccacgactg aacgcaagcc gcgtacggat acaacgacca
     1861 cgcgcgacgc gacggtgacc gtcctgccgg ccaacgaggc gtcatgggac gacctgcaga
     1921 cggtcttccg cgaaggcgga gacgccgccg gatgccagtg ccagtggttc aagctacagc
     1981 gcgccgagtg gcgctcgacg ccgcgtgccg aacggacggc cctcctaagg caacagacag
     2041 cctgcggcca cgccgaggcc acccacacca cgggcttggt cgcgtacctg gacggtgagc
     2101 ccgccggttg gtgcgccgtc gagccgcgca cggcctaccc gcggctgctc acagcccggg
     2161 ttccctgggc cggccgcgac gaggtcaggt cggatgagac cgtgtgggcc ataacctgct
     2221 tcgtgacccg ccaaggcttc cgcagcaagg gcgtgagccg cgccctcgcg cgcgccgccg
     2281 tcgagttcgc gcggagccgc ggcgccagcg ctgtcgaagg ctacgcgcgt cgcactaacc
     2341 cgggcgccaa gcagtccccg ggcccgctct tcgtcggaac gccgagcatc ttcgctgccg
     2401 ccgggctccg agaggtgagc cagccgacgc ctgggcgcgt cgtcatgcgg atcgacttcg
     2461 gggggtgagg gccgggccgg tcgaacgccg gcacggttcg gctcggcagt ctagcagc
//
LOCUS       DAOQYE010000233         2515 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_45953_length_2515_cov_2.566260, whole genome shotgun sequence.
ACCESSION   DAOQYE010000233 DAOQYE010000000
VERSION     DAOQYE010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2515
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45953_length_2515_cov_2.566260"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            440..742
                     /locus_tag="PKN52_04765"
     CDS             440..742
                     /locus_tag="PKN52_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99288.1"
                     /translation="MRCATFDQDAVQDGEVVDRDVAVEGLVGLFAGRVLAFDTAAARR
                     YADLAVAARNAGRGFPTSDGYIAAIATANGFAVATCDEAAFEAAGVPFIDPWKVRG"
     gene            complement(766..1431)
                     /gene="rdgB"
                     /locus_tag="PKN52_04770"
     CDS             complement(766..1431)
                     /gene="rdgB"
                     /locus_tag="PKN52_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HNQ99289.1"
                     /translation="MTASPDTARIRIAVATQNAGKLREFADALAGLPFELVSAGDLGV
                     TEFPEENGATYEENALIKAGHVAVTTGLPALSDDSGLEVDALDGAPGVYTARFGGHGL
                     TDGERMAHLLQRLRKVSDAERTARFVAVIVLATPAGAVEVFRGESEGRVLQGPRGERG
                     FGYDPIFFSTELGKGFAEATLAEKRGVSHRGRALAAFAAWAMTPTGTEVLLDTTPRQL
                     DFD"
     gene            complement(1428..2150)
                     /gene="rph"
                     /locus_tag="PKN52_04775"
     CDS             complement(1428..2150)
                     /gene="rph"
                     /locus_tag="PKN52_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HNQ99290.1"
                     /translation="MSAPKREGRAATELRPVKVELGYNPHAEGSALVTWGRTVIAATV
                     TIEVKLPPHLRATAHKGGWLTAEYALLPRSTKERVVRERLYASGRTQEIQRLMGRALR
                     STVDLDLFKGKTITVDADVLVADGGTRCAAILAGYAALHQAADKLVFQGGVAEWPLRH
                     EVAAVSVGVVDGETRVDLDHAEDAAAELDLNVVATASGDVLEVQGGSETGPVAAERYV
                     ALVGAGVAAVERLVAQVRGQLK"
     gene            complement(2195..>2515)
                     /locus_tag="PKN52_04780"
     CDS             complement(2195..>2515)
                     /locus_tag="PKN52_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012969185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HNQ99291.1"
                     /translation="NDIMPAIANMDADVISIETSRSRMELLGAFKSFKYPSEIGPGVY
                     DIHSPRVPSTDEILRLLRLARELLADEQLWVNPDCGLKTRGWAEVRPALVNMVAAAKE
                     LRGA"
BASE COUNT          354 a          852 c          886 g          423 t
ORIGIN      
        1 gcgcgcacgc acagcatcac gatgggctac cgctcgcgcg tcatccgctt cacggactcc
       61 gtccacctgc tcggggacgg cgcgcgcgtg accatccagg tgtgaggctc ggttaaggcc
      121 tgttcgctct tgacggcgtg gcgtcaggcg tcacgtacga ttgagattcg atctggggtg
      181 aaccgcgggt ctcctttcac gcgttgaccg gactcggcaa ggtgttcggc aggtgcatgt
      241 tccttggcgg ttgaagagtc actgccacgc cccaccgggc ttctgaccgt gcagcgcatc
      301 aggtgcagca cggttacctt ccaggacgct gaggtgttgg gcgaaaaacg acaggtcatg
      361 caacgaggca gttgcgcaat catctggttg cgcaagtagg cagttctcaa gaagtactta
      421 ggctccccga ccccgggggg tgagatgtgc aacgttcgac caagatgccg ttcaggacgg
      481 tgaggttgtc gatcgcgacg ttgcggtcga ggggctcgtc ggtctgttcg ccggcagggt
      541 tctcgccttc gacaccgcgg ccgccaggcg ctacgcagac ctggccgtcg ccgctcgcaa
      601 cgcgggcagg ggcttcccga cgtcggacgg ctacatagcg gccatcgcga ccgccaacgg
      661 cttcgccgtg gcgacgtgcg acgaggccgc tttcgaagcc gccggcgttc cgttcatcga
      721 cccgtggaag gtccggggct gaccgcggcg ccagcgtggc ctcggtcagt cgaagtcgag
      781 ctggcggggg gtcgtgtcta gcaggacctc ggtcccggtc ggcgtcatgg cccacgcggc
      841 gaaggccgcc agggcgcgcc ctcggtggct cacgccgcgc ttctcggcca gggtcgcctc
      901 agcgaagccc ttgcctagct ccgtgctgaa gaagatgggg tcgtaaccga agccccgctc
      961 gccacgcggg ccttgcagca cgcgcccctc gctctcgccc ctgaagacct ctacggcccc
     1021 cgccggcgtg gcgagcacga tgacagcgac gaaccgggcc gtgcgctccg cgtccgacac
     1081 cttccttagc cgctgcagca ggtgcgccat gcgctcgccg tcagtgaggc cgtgtccgcc
     1141 gaagcgggcc gtgtagactc cgggagctcc gtccaaggcg tcgacctcga ggcccgagtc
     1201 gtctgataac gccggcaacc ccgtagtcac cgcgacgtgc ccggccttga tgagggcgtt
     1261 ctcctcgtag gtggcgccgt tctcctccgg gaactcggtt acgcccaggt ccccagccga
     1321 gaccagttcg aaggggaggc cggccagcgc gtcagcgaac tcgcggagct tgcccgcgtt
     1381 ctgggtggcg acggcgatgc gtatgcgagc cgtgtccggc gaggccgtca cttgagctgt
     1441 cctcgcactt gggcgacgag gcgctccacc gccgcgacgc ccgcgcccac gagcgcgacg
     1501 tagcgctcgg ccgcgaccgg gcccgtctcg ctgccgccct ggacctcgag cacgtcgccg
     1561 gaggccgtgg cgaccacgtt gaggtcgagt tcggccgcgg cgtcttccgc gtggtcgagg
     1621 tcgacccgcg tctcgccgtc caccaccccg acgctcacgg ccgcgacctc gtggcgcagc
     1681 ggccattcgg cgacgccgcc ctggaacacg agcttgtcgg ccgcctggtg gagggcggcg
     1741 tagccggcga ggatggcggc gcagcgtgtg ccgccgtctg ccacgagcac gtcggcgtcg
     1801 acggtgatgg tcttgccctt gaacaggtcg aggtcgacgg tgctgcgcag agcccggccc
     1861 atgaggcgct ggatctcctg ggtgcgaccg gaggcgtaga gccgttcgcg cacgacgcgc
     1921 tccttggtgc tgcgggggag gagggcgtac tcggccgtga gccagccgcc cttgtgcgcg
     1981 gtggcacgta ggtgcggtgg cagcttgacc tcgatggtca cggttgccgc gatcacggtg
     2041 cgaccccacg tgaccaacgc cgacccttcg gcgtgggggt tgtagccgag ctctaccttg
     2101 acgggcctca gctcggtggc ggcgcgacct tcgcgcttcg gtgcgctcat gacggggagc
     2161 ttatcaccgc cctcggagga gcgcctcccg ccggttaggc gccccgtagc tccttggcag
     2221 ccgcgaccat gttgacgagg gcgggccgca cttccgccca accgcgggtc ttgagcccgc
     2281 agtcggggtt cacccagagc tgctcgtccg ccagcagttc tcgggcgagg cggagcaggc
     2341 gcaggatctc gtccgtgctc ggcacgcgcg gggaatggat gtcgtagacg cccggcccga
     2401 tctcgctcgg gtacttgaac gacttgaacg ccccgagcag ctccatcctg gagcgcgaag
     2461 tctcgatgga gatgacgtcg gcgtccatgt tggcgatggc gggcatgatg tcgtt
//
LOCUS       DAOQYE010000234         2495 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_46507_length_2495_cov_2.756148, whole genome shotgun sequence.
ACCESSION   DAOQYE010000234 DAOQYE010000000
VERSION     DAOQYE010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46507_length_2495_cov_2.756148"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1036
                     /locus_tag="PKN52_04785"
     CDS             <1..1036
                     /locus_tag="PKN52_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HNQ99292.1"
                     /translation="ALVANTEYAGEIKKGLFTALNLELPRRDVFPMHCSANEGQDGAV
                     TLFFGLSGTGKTTLSTDPSRRLIGDDEHGWSGTGVFNFEGGCYAKVVNLTEEAEPDIY
                     AASSRFGTVLENVKLSGEREPDYADTSKTENTRAAYPISFVSNASPTGVGGHPRDIVF
                     LSADAFGVLPPIARLTVPQALYYFLNGYTAKLAGTERGVTEPVATFSTCFGAPFMPLS
                     PLEHAEMLRTRLAAHSPRVWMVNTGWTGGGFGVGKRISLRYTRAMLEAALSGALDEVR
                     TVEDGVFGLLVPQHVPNVPDSLLSPRGTWLNATTYDAEARRLAGMFRKNFERFAAEVA
                     ADVAAAGPRG"
     gene            complement(1093..2433)
                     /locus_tag="PKN52_04790"
     CDS             complement(1093..2433)
                     /locus_tag="PKN52_04790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNQ99293.1"
                     /translation="MKRRWYIAGLVVLVAATVMVVNAQLSRDYASEFLNNPTGRALVQ
                     AFGALKSGYLNDVDDDALIQGAIKGMLESLDDPYTSYLLPKDAARDSQDLTGSFEGIG
                     AVLTPHDRQSGKGVEILTVYAGGPAAGAGLMRGDVFLEVEGVDVRELTTSEVADLVRG
                     PKGTTVNLVMERPGVQDPVTFNIQRSTIQIVDVSSAMLPNGVGYIALASFNNQRLHDQ
                     LVEHLDKLILDGAKSLVLDLRDNSGGLLNQAILIADEFLSKGDIVFQRSRGVTQRIAS
                     ADQHAYDLPMVVLVNENSASASEIVAGALQENDRALVVGEQTFGKGVAQSVLSLSDGG
                     QLRYVSFEWLTPLRRSIAEKGITPDVYAPDTRYPRTISVDGQGLRDGQTVQLVVDGEV
                     VGEAVSGDDGKFRILALGGVREVSAVQGEAIVHLDTDTALQTAVSALEGQLTKR"
BASE COUNT          368 a          851 c          870 g          406 t
ORIGIN      
        1 cgcgctcgtc gccaacaccg agtacgcggg cgagataaag aaggggctct tcacggccct
       61 gaacctcgag ttgccgcgcc gggacgtgtt ccccatgcac tgctcggcca acgagggcca
      121 ggacggggcc gtgacgctct tcttcggcct ctccggcacc ggcaagacga cgctgtcgac
      181 cgacccgtcg cggcgcctca tcggcgacga cgagcacggc tggtcgggga ccggcgtgtt
      241 caacttcgag ggcggctgct acgccaaggt cgtcaacctg accgaggagg cggagcccga
      301 catctacgct gccagttcgc gcttcggcac ggtgctggag aacgtcaagc tgagcggcga
      361 gcgcgagccg gactacgccg acacctccaa gacggagaac acgcgcgccg cctaccctat
      421 ctcgttcgtc tccaacgcct ccccgacggg cgtcggcggg cacccgcgcg acatcgtctt
      481 cctgagcgcc gacgcgttcg gggtgctccc gcccatcgcc aggctcaccg tgccgcaggc
      541 gctgtactac ttcctgaacg gctacacggc caagctggcc ggcaccgagc gcggcgtgac
      601 ggagcccgtg gcgacgttca gcacctgctt cggcgcgccc ttcatgcccc tgtcgcccct
      661 cgagcacgcc gagatgctgc gcacccgcct ggccgcgcac tccccgcgcg tgtggatggt
      721 caacacgggc tggactggcg gcggcttcgg ggtgggcaag cggatctcgc tgcgttacac
      781 gcgcgccatg ctggaggcgg ccctctcggg cgcgcttgac gaggtgcgga cggtcgagga
      841 cggcgtcttc ggccttctcg tcccgcaaca cgttcccaac gtgccggact ccttgctgag
      901 cccgcgcggc acgtggctca acgcgaccac ttacgacgcc gaggcgcggc gtctggccgg
      961 catgttccgg aagaacttcg agcgcttcgc cgccgaggtg gccgccgacg tggcggcggc
     1021 tggacctcgg ggctgaccag ggctgacttg ctggcgctcg caggctgact tgctagcgcg
     1081 ctcgctgagc ccttaacgct tggtgagctg gccttccagc gcgctgacgg cggtctgcag
     1141 cgccgtgtcg gtgtcgaggt gcacgatggc ctcaccctgc acggcgctaa cttcgcgcac
     1201 gccgccgagg gcgaggatgc ggaacttgcc gtcatcgccg gagaccgcct cgccgacgac
     1261 ctcgccgtcg acgacgagct ggaccgtctg accgtcgcgc aggccctgcc cgtcgacgga
     1321 gatggtgcgg gggtagcgcg tgtcgggcgc gtacacgtcg ggcgtgatgc ccttctcggc
     1381 gatgctgcgg cgcagcgggg tgagccactc gaacgacacg tagcggagct ggccgccgtc
     1441 cgagagcgag agcacggact gggccacgcc cttgccgaac gtctgctcgc cgacgacgag
     1501 cgccctgtcg ttctcctgga gcgcccccgc gacgatctcc gacgccgagg ccgagttctc
     1561 gttgacgagc acgaccatgg gtaggtcgta cgcgtgctgg tcggccgacg cgatgcgctg
     1621 ggtgacgccg cgcgagcgtt ggaacacgat gtcgcccttg ctcaggaact cgtcggcgat
     1681 gaggatggcc tggttgagca ggccgcccga gttgtcgcgc aggtcgagga cgagcgactt
     1741 ggcgccgtcc aggatcagct tgtcgaggtg ctcgacgagc tggtcgtgca ggcgctggtt
     1801 gttgaacgac gccagggcga tgtagccgac gccgttcggg agcatggcgc tcgagacgtc
     1861 gacgatctgg atggtcgagc gctggatgtt gaacgtgacc gggtcctgga cgcccgggcg
     1921 ctccatcacc aggttcacgg tcgtgccctt cgggccgcgc acgaggtcgg cgacctcgct
     1981 cgtggtgagc tcgcgcacgt cgaccccctc gacctcgagg aacacgtcgc cgcgcatgag
     2041 gccggccccg gcggcagggc cgccggcgta gaccgtcagg atctcgacgc ccttgccgct
     2101 ttgcctgtcg tgcggcgtga ggacggcgcc gatgccctcg aaggagcccg tgaggtcctg
     2161 gctgtcgcgg gcggcgtcct tgggtaggag atagctcgtg tacgggtcgt cgagcgactc
     2221 gagcatgccc ttgatggcgc cctggatcag ggcgtcgtcg tcgacgtcgt tgaggtagcc
     2281 gctcttgagc gccccgaagg cctgcaccaa cgcgcggccg gtagggttgt tgaggaactc
     2341 gctcgcgtaa tcgcgagaca gctgagcgtt cacgaccatc accgtggccg cgaccagcac
     2401 cacaagaccc gcgatgtacc atctgcgctt catgaagcct ccgtctgttc gccctgcatg
     2461 cggcctagag tataagggcc ttacatgaga gccta
//
LOCUS       DAOQYE010000235         2492 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_46590_length_2492_cov_3.325810, whole genome shotgun sequence.
ACCESSION   DAOQYE010000235 DAOQYE010000000
VERSION     DAOQYE010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46590_length_2492_cov_3.325810"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(144..1085)
                     /gene="fabD"
                     /locus_tag="PKN52_04795"
     CDS             complement(144..1085)
                     /gene="fabD"
                     /locus_tag="PKN52_04795"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HNQ99294.1"
                     /translation="MSGVPGVVAALFPGQGSQAVGMARELYEASPAAKRVLDEAEAAL
                     PGLLALMWEGPAGELQLTANQQPALVAAGAAAFAAYLEAGGVAPRLAAGHSLGEYTAH
                     VASGTLSVADAVRTVRKRGQYMQDAVPAGAGAMAAILKLDEGVIREVLAEVAAAGATD
                     LVAVANLNAPGQTVISGTASGVEKATAALKARGGRAIPLKVSAPFHSPLMEPAAARLA
                     PDLAGLTFREPGLTVVCNVTAEPLAGASQAARLLTEQVTAPVRWVECVEKLASLGASR
                     YIEFGSGNVLVGLLGRILPDASGVAVTDPASLAAALA"
     gene            complement(1082..2071)
                     /locus_tag="PKN52_04800"
     CDS             complement(1082..2071)
                     /locus_tag="PKN52_04800"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HNQ99295.1"
                     /translation="MSETRPRAGVTALGAYAPSKVVTNFDLEKIMDTSDEWITTRTGI
                     KRRHIAADDEFTSDLAFRAVENLVARHGEHALDGVDMVILATNTPDALFPATAALVQE
                     KFRLRAGAFDLLAACPGWLYAVGIAKSLVESGQCKRVLAIGAEALTKIVDWGDRSTAV
                     LFGDGGGAAIIAAVPEPYGVKGVVLGADGSGADHLHKRSVGVCLPSGARLSDSLYMNG
                     REVYKFAVRVMNTATLEAVEKAGLAVDDISLFVPHQANLRIIEAARERLALPKERVVV
                     TVDEYGNNSTASIPLALAHALDTGRINVGDNLLLVSFGAGLTWASAVLTWGPA"
     gene            complement(2217..2402)
                     /gene="rpmF"
                     /locus_tag="PKN52_04805"
     CDS             complement(2217..2402)
                     /gene="rpmF"
                     /locus_tag="PKN52_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HNQ99296.1"
                     /translation="MAKHPVPKKRTSRSSRDARRSHHALTAPTLVACPQCKTMKPPHV
                     VCPECGSYDGRQVVAEA"
BASE COUNT          342 a          860 c          930 g          360 t
ORIGIN      
        1 gagacctcct aggaagttga ccttatggtg cccggtgtgg gcgactcgct acggccggcc
       61 cgttatgggc gacccgaggc gtgcgaccag gcgcgcgggt ccgcatcagg cggcccggca
      121 tggccttggc cacgcgtgag ccattatgcg agagcggccg cgaggctggc ggggtcggtc
      181 acggccacgc cgctcgcgtc tggcaggatg cgcccgagca gtccgaccag cacgttgccg
      241 gaaccgaact cgatgtagcg gctcgccccg agcgaggcga gcttctcaac gcactcgacc
      301 caccgcacgg gcgccgtcac ctgttcggtg aggagccggg cggcctgcga agccccagcg
      361 agaggttcgg cggtcacgtt gcacacgacg gtgaggccgg gctcgcggaa ggtcaacccg
      421 gccaggtccg gtgccaggcg cgcagcggcg ggctccatga gcggagagtg gaagggcgcg
      481 ctgaccttca gcgggatggc gcggccgccc ctggccttga gggccgccgt cgccttctcg
      541 accccggagg cggtgccgga gatgaccgtc tgaccgggcg cgttcaggtt ggcgaccgcc
      601 actaggtcgg tcgcaccggc cgcggcgacc tcggcgagca cctcgcggat gaccccctcg
      661 tccagcttca ggatggcggc catggcgccg gcgccggccg gcaccgcgtc ttgcatgtac
      721 tgcccacgct tgcgcacggt gcgcacggcg tccgccacgc tcagcgtgcc ggacgcgacg
      781 tgggccgtgt actcgcctag cgagtggccg gccgccaggc gcggggcgac cccacccgcc
      841 tcgaggtagg cggcgaacgc ggcggcgccc gccgccacca gcgcgggctg ctggttcgct
      901 gtcagttgca actcgccggc ggggccttcc cacatgagcg cgagcaggcc gggcagggcc
      961 gcctccgcct cgtcgagcac gcgcttggcg gccggggagg cctcgtagag ctcccgagcc
     1021 atgcccacgg cctgggagcc ctggccgggg aagagggcgg cgacgacgcc cggtacgccg
     1081 ctcacgccgg cccccaggtg aggacggccg acgcccacgt gaggccggcg ccgaacgaga
     1141 cgaggagcag gttgtcgccc acgttgatcc tgccggtgtc gagcgcgtgc gcgagggcga
     1201 gcgggatgct ggcggtggag ttgttgccgt actcgtcgac ggtgacgacg acgcgctcct
     1261 tgggaagggc gaggcgctcg cgcgccgcct cgatgatgcg caggttcgct tggtggggga
     1321 cgaagaggga gatgtcgtcc accgccaaac cggccttctc gaccgcctcg agggtggccg
     1381 tgttcatgac cctgacggcg aacttgtaga cctcgcggcc gttcatgtag agcgagtcgg
     1441 agagccgcgc gccgctcggc aggcacacgc cgacggagcg cttgtggagg tgatcggcgc
     1501 cggagccgtc ggcgccaagc accacgcctt tcacgccgta cggctccggc actgccgcga
     1561 tgatggcggc gccgccgccg tcgccgaaca gcaccgccgt cgaacggtcg ccccagtcga
     1621 cgatcttggt cagcgcttcc gcgccgatgg cgaggacgcg cttgcactgc cccgactcca
     1681 cgaggctctt ggcgatgccg accgcgtaca gccagcccgg gcaggccgcg aggaggtcga
     1741 acgcgccggc cctgagccgg aacttctcct ggacgagggc ggcggtggcg gggaagaggg
     1801 cgtccggcgt gttagtggcc aggatgacca tgtcgacgcc gtccagcgcg tgctcgccgt
     1861 gccgcgccac caggttctcg acggcgcgga aggccaggtc ggaagtgaac tcgtcgtccg
     1921 ccgcgatgtg cctgcgcttg atgcccgtcc gggtggtgat ccactcgtcg gacgtgtcca
     1981 tgatcttctc gaggtcgaag ttcgtcacga ccttggacgg cgcgtaggcg ccgagggccg
     2041 tgacaccggc acgcggtctg gtctcgctca cgcctaccta cgttccgtcg gccggcgcgc
     2101 cggtccagcc gcgctgccac aagagaaagg acggccggag ccgtcctggc gcggccacgc
     2161 tcggccgccg tcgagattgc cggccgccag ccgtcctgcc agcggccgta agtgcctcaa
     2221 gcttccgcga ccacctggcg gccgtcgtag ctcccgcact cggggcacac gacatgcggc
     2281 ggcttcatcg tcttgcactg tgggcaagcc acgagggtag gcgcggtcag cgcgtggtgc
     2341 gagcggcgcg cgtcgcggct ggagcgcgag gtgcgcttct tgggtactgg gtgcttagcc
     2401 atctggtcct gctccttata agtggcgctc gccgtgagcg gcgagtgacg ctcgagtgta
     2461 gtgtcccgac cgcttcggtg cgggtcaggc gg
//
LOCUS       DAOQYE010000236         2492 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_46604_length_2492_cov_1.979073, whole genome shotgun sequence.
ACCESSION   DAOQYE010000236 DAOQYE010000000
VERSION     DAOQYE010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46604_length_2492_cov_1.979073"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            250..837
                     /locus_tag="PKN52_04810"
     CDS             250..837
                     /locus_tag="PKN52_04810"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNQ99297.1"
                     /translation="MEAGARTQLFIEPYPSATPEVGLWLGGLQHIRQALLRTVERIDA
                     GGLGQEFIDWRGVNGDDNSVGSLLYHVAGIELGWLYTDLLMQEFPPYVLELFPYPDRD
                     ADGALQHVPGIALSEHVARLNTSRARFLEVVGGLDLAAWRRMGEPAGEEYAVTPEWIV
                     WHLIEHEAGHLYAIRGTVRKWLAQSSSGTNATANR"
     gene            complement(798..1571)
                     /locus_tag="PKN52_04815"
     CDS             complement(798..1571)
                     /locus_tag="PKN52_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99298.1"
                     /translation="MTEADSGRERKARVSDSRAPSAAAPDRSGRNEPVPGSARDRGAA
                     DRAYHRVTNAEQARLLTEPAYDDAFRPFLARECSVGEAAAEAGLTLDAMLYRVRVLLK
                     AGLLEVARVKPRAGRPIKLYRSVHDAYFIPYELTPFASLEERLWRQFMPELKERLAIQ
                     ARRLRERGFDGQRLYRNDFGETFMESAPDALSPVDFLDSEREPALDFWTNVALTEQRA
                     REVQRTLYDLWRRSQEPEAEVGDGKRRYRLAVAFVPLDD"
     gene            1602..>2492
                     /locus_tag="PKN52_04820"
     CDS             1602..>2492
                     /locus_tag="PKN52_04820"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNQ99299.1"
                     /translation="MSVRATYYLVSSLNWFANVVPMAVSVLFAQSRGLSLGQIGVYGA
                     AYSLTIVLLELPSGALADTFGRKRTYLLAGALALGAKVLFLMAFDLAGFVAYALLWGT
                     ARALSSGALEAWFVDALQAREPDAPLQSALAAAGSYNLVGLAAGTVTGAMLPGLFAWL
                     PSDGAAVLTPLSVTVIVSAAAQAAVIALAALLVREPRPDGERVGGRSGLRSALAEVRT
                     VMLDAAALTRASRPLQLLLLGECLVGVALMTSETLWQPFFATRISEARLGAVSSGVGN
                     ITAQLLGTSAGADARVLGFVL"
BASE COUNT          351 a          878 c          858 g          405 t
ORIGIN      
        1 agcgcgccga tcccgacgcg ccaagtgacg gcgccggccg gcgaccaacc tgccactagc
       61 agacccttgg cgagcgaccc cgacgtgccg aacgcggcgg aggacgccaa agcgagtgcc
      121 agccccaacc agttgtagcg gttcatggaa gccttctccc gagcgttccg cgtctatggc
      181 ccgaacgtcg tgtgcgacgc cctgagtgag gaccgcgccg cgaccgctat attacgcggc
      241 ggaggaccca tggaagcagg agcacggacg caactcttca tcgagccata cccgagcgcc
      301 acgcccgagg tcgggctgtg gctcgggggg ttgcagcaca tcagacaggc cctccttcgc
      361 accgtcgagc gcatcgacgc cggtggcttg ggccaggagt tcatcgactg gcgcggcgtg
      421 aacggcgacg acaactccgt cggctcgctc ctctatcacg tggccggcat cgagttgggg
      481 tggctttaca cggacctgct gatgcaggag ttcccgccct acgtgctaga gctgttcccg
      541 taccctgacc gcgacgcgga cggcgccctc cagcacgtgc ccggcatagc cctgagcgag
      601 cacgtcgccc gtctgaacac gtcgcgcgcg cgtttcctcg aggtggtcgg cgggcttgac
      661 ctggccgcgt ggcggcgcat gggcgaaccg gccggcgagg agtacgccgt gacgccggaa
      721 tggatcgtgt ggcacctcat cgagcacgag gccgggcacc tgtacgcgat caggggcacg
      781 gtgaggaagt ggctggctca gtcgtcgagc ggcacgaacg cgaccgccaa ccggtagcgc
      841 ctcttcccgt ctcccacttc ggcctccggc tcctgcgagc gacgccatag gtcgtagagg
      901 gtgcgctgca cttcgcgcgc tcgctgctcg gtcagcgcga cgttggtcca gaaatccaag
      961 gccggctcgc gctccgagtc caggaagtcg acggggctga gcgcgtccgg cgcggactcc
     1021 atgaacgtct ccccaaagtc gttgcgatag aggcgttggc cgtcaaagcc ccgctcgcgc
     1081 agtcggcgcg cctggatggc gagccgctcc ttgagttcgg gcatgaactg ccgccagagc
     1141 cgctcttcca ggctcgcgaa cggcgtcagc tcgtagggga tgaagtaggc gtcgtgcacg
     1201 gagcggtaga gcttgatcgg gcgtcctgcc ctgggcttga cgcgggccac ctcaagcaag
     1261 cctgccttta gcaacacccg aacgcggtag agcatggcgt cgagcgtcag tcccgcctcg
     1321 gccgctgcct cgccgaccga gcactcacgc gcgaggaacg gacggaaggc gtcgtcgtag
     1381 gcgggctccg tgaggagacg cgcctgctcc gcgttggtca ccctgtggta agcgcggtcg
     1441 gcggcgccgc ggtcgcgggc ggagccgggg acgggctcgt tcctacctga ccggtccggc
     1501 gccgctgcgg agggcgctcg cgagtcggat acccgggcct tacgttccct acctgaatca
     1561 gcctctgtca tcacaagtag cgtaaccgcc acgctctgcc catgagcgtt cgcgccacct
     1621 actacctggt gagttccctc aactggttcg ccaacgtcgt gcccatggcc gtctccgtcc
     1681 tgttcgcgca gtcgcgcgga ctgtcgctcg gacagatcgg cgtctatggc gcggcctact
     1741 ccctcacgat cgtgctcctg gaactgccct ccggcgcgct ggccgacacg ttcggacgca
     1801 agcgcacgta cctgctcgcg ggtgcgctcg ctctcggcgc caaggtcctg ttcctcatgg
     1861 cgttcgacct ggccggcttc gtcgcctacg cgctcctctg gggcactgcg cgcgccctca
     1921 gttccggggc gctggaagcg tggttcgtcg acgcgttgca ggcgcgcgag ccggacgctc
     1981 cgctccagag cgcgctcgcg gcggcgggct cgtacaacct cgtcggcctc gccgccggga
     2041 cagtgacggg cgcgatgctg cccggcctct tcgcgtggct gccgagcgac ggcgccgcgg
     2101 tgctcacccc gctgtcggta acggtgatcg tctcagcggc ggcgcaggcc gcggtcatcg
     2161 ccctcgccgc gctactggtc cgcgagccgc ggccggacgg ggagcgggtc ggtgggcgat
     2221 ccggactacg ctccgcgctc gcggaggtac gcacggtcat gcttgacgcg gccgccctca
     2281 cgcgcgccag ccggccgctg cagttgctgc tcttgggcga atgcctggtc ggggtcgcgc
     2341 tcatgacctc cgagacgctc tggcagccgt tcttcgccac tcggatcagc gaggcgcggc
     2401 tcggcgcggt gtcgtcgggc gtcggaaaca tcacggccca gctcctcggc acctccgccg
     2461 gagcggacgc gcgcgtgctc gggttcgtgc tc
//
LOCUS       DAOQYE010000237         2486 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_46768_length_2486_cov_3.037022, whole genome shotgun sequence.
ACCESSION   DAOQYE010000237 DAOQYE010000000
VERSION     DAOQYE010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46768_length_2486_cov_3.037022"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="PKN52_04825"
     CDS             <1..381
                     /locus_tag="PKN52_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99300.1"
                     /translation="DPVKADSDLRLGYSYARTTFEPTVRQLAEGLWQERQLRPPVVAP
                     RGSGDARRRSPAPQGGGRFDDQGGNLVDGRPGQQGDGRRGDSDQKGRRGNRRGRRGGG
                     QAGGQGGAQGGGPDNGRAGGSSGG"
     gene            463..1599
                     /locus_tag="PKN52_04830"
     CDS             463..1599
                     /locus_tag="PKN52_04830"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HNQ99301.1"
                     /translation="MSESAILQAAYAAEAPYLELLADLVTHESPTDDKAACDALAARL
                     AEHMRASGLNVTRHEQADAGAILEGRLAGAGGGKTLLLCHYDTVWPLGTLASMPFTRS
                     GDKVSGPGSVDMKAGIANALTALRLLEELGLRPAGDVTLLVTSDEETGSHASRELIEA
                     RAREHDRVFVLEPGRDDGALKVGRKGVGGFRLKFRGRSAHAGNNPSDGASALRELAHM
                     LVFIEDLSDDVAGTTVNVTVARAGFATNVIAEEAEAFVDFRVLKASEAERVTNAINDY
                     TPRDSRVTLTVTGGLNRPPLELTPANRALFERAQAIGNELGLVFDGGVVGGGSDGNFT
                     SAIGVPTLDGLGSVGGGPHARHEHIRVRESLQRVALLTGLLASD"
     gene            1606..>2486
                     /locus_tag="PKN52_04835"
     CDS             1606..>2486
                     /locus_tag="PKN52_04835"
                     /inference="COORDINATES: protein motif:HMM:NF039484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99302.1"
                     /translation="MPLLLAAACGLLLTLALPPLGWWPFAFALAPVFALVARAKDGRA
                     AFRVGAAFGLPFFALYILWLPRSFAVLFGPAFWFVYPFLLAILAVFWGLTAWGTWRVA
                     GLAGGGSSPRGRAKRVLLLLPVAWVFVEWARTQGYFAFPWGTLGYAWLETPVAQLAEF
                     GGVYVLSLLVTAPAALLAWPWAVGARPGRGGSGQARGATVACCVVAVALVGAAFMVGS
                     AVQRGAPTLAAEAASAPYRALLVQGNVDPFARAASSAQELGVHLRLTEDGDTASGPFD
                     LVVWPEGAVIGYDLTAG"
BASE COUNT          310 a          920 c          897 g          359 t
ORIGIN      
        1 gacccggtca aggccgacag cgacctgcgc ctcggctaca gttacgcccg cacgactttc
       61 gagcccaccg tcaggcagct cgcggagggc ctctggcagg agcggcaact gcggccgccg
      121 gtcgtggcgc cccgcggctc gggtgacgcc aggcggcgct ccccggctcc ccaaggtggc
      181 gggcggttcg acgaccaggg cggcaacctg gtcgacggca ggccggggca gcagggggac
      241 ggtcgccgcg gcgactccga ccagaagggt cgccgcggca accgccgcgg gcgccgcggg
      301 ggtgggcaag ctggcgggca aggcggcgca caaggtggcg gccccgacaa cggccgagca
      361 ggcggctcga gcggcggctg acggaccccg ccccgatacc gccggccagg cggttgccaa
      421 gcgggcgcca agcgggcgcc ttaggggggt aacatcggcg ccatgagcga gtccgccatc
      481 ctgcaagccg cctacgccgc cgaggctcct tacctggagc tgctcgccga cctggtcacg
      541 cacgagagcc cgaccgatga caaagccgcg tgcgacgcgc tggccgcacg cttggccgag
      601 cacatgcggg cgagcggctt gaacgtcacg cggcacgaac aggccgacgc cggcgccatc
      661 ctggaagggc gcctggcggg cgccggcggc ggcaagacgc tcctcctctg ccattacgac
      721 acggtgtggc ccctcggcac cctggcctcc atgccgttca cccgctcggg agacaaggtc
      781 tcggggcccg gctcggtcga catgaaggcc ggcatcgcca acgcgctgac cgcgctgcgg
      841 ctactcgagg agctcggtct ccgtccggcc ggcgacgtca cgctgttggt gacgagcgac
      901 gaggagacgg gctcccacgc ctcgcgcgag ctcatcgaag ccagggcgcg cgagcacgac
      961 cgcgtgttcg tgttggagcc cgggcgcgac gacggcgccc tgaaagtcgg ccgcaagggc
     1021 gtgggcggct tccggctcaa gtttcgcggc cgttccgcgc acgccggcaa caacccttcc
     1081 gacggcgcga gcgcgcttcg tgagctggcg catatgctcg tgttcatcga ggacctgagc
     1141 gacgacgtgg caggcaccac cgtcaacgtg accgtggccc gcgccggctt cgctaccaac
     1201 gtgatcgcgg aggaagcgga ggcgttcgtc gacttccgcg tgctcaaggc cagcgaggcc
     1261 gagcgcgtca ccaacgccat caacgactac acgccccgcg actcacgcgt gaccctcacg
     1321 gtcacgggag gcctcaaccg cccgcccctc gaactcaccc ccgccaaccg ggccctgttc
     1381 gagcgcgccc aggccatcgg gaacgagctg ggtctggtgt tcgacggcgg cgtcgtcggg
     1441 ggcggctccg acggcaactt cacgtccgcc atcggcgttc cgacgctaga cggcctcggt
     1501 tccgtcgggg gtgggccgca cgcccgccac gagcacatcc gcgtgcgcga gtctctccaa
     1561 cgcgtagcgc ttctcacggg gttgctcgcg agcgactgat ccgacatgcc gctgctgcta
     1621 gccgcggctt gcggcctgct cctcacgctc gccctgccgc cgctgggatg gtggccgttc
     1681 gccttcgcgc tcgcgcccgt cttcgccctc gtggcgcgcg ccaaggatgg ccgcgcggcg
     1741 ttccgcgtcg gagcggcgtt cggcctgccg ttcttcgccc tctacatcct gtggctgccg
     1801 cgctcgttcg cggtgctgtt cggcccggcc ttctggttcg tctacccgtt cctcctcgcc
     1861 atcctggcgg tcttctgggg cctcacggcg tgggggacgt ggcgcgtcgc cggcctcgcc
     1921 ggagggggct cttccccgcg cggccgggcc aagcgcgtcc tcctgctcct ccctgtcgcc
     1981 tgggtgttcg tcgagtgggc acgcacgcag ggctacttcg ccttcccttg gggcacgctc
     2041 ggctacgcct ggctggagac gcccgtcgcg caactcgccg agttcggggg agtgtacgtg
     2101 ctgagcctgc tcgtcaccgc gcccgccgcg ctgttggcgt ggccgtgggc ggtgggcgcg
     2161 cgcccgggtc ggggcggcag cggccaggcc cgcggtgcga ccgtcgcatg ctgcgtagtg
     2221 gccgtggctc tcgtaggggc ggcgttcatg gtgggctcgg ctgtccaacg cggcgcgccc
     2281 actctggcag cggaggcggc ctccgccccc taccgcgcgc tgctcgtcca gggcaacgta
     2341 gacccgttcg cgcgcgcggc ctcgtcggcg caggagctgg gcgtgcacct gcgcctcacg
     2401 gaggacggcg acaccgcctc cggcccgttc gacctcgtgg tgtggccgga gggtgccgtc
     2461 atcggctacg acctgacggc cggcga
//
LOCUS       DAOQYE010000238         2480 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_46939_length_2480_cov_3.325361, whole genome shotgun sequence.
ACCESSION   DAOQYE010000238 DAOQYE010000000
VERSION     DAOQYE010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46939_length_2480_cov_3.325361"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..826)
                     /locus_tag="PKN52_04840"
     CDS             complement(<1..826)
                     /locus_tag="PKN52_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99303.1"
                     /translation="MTTLALTLVFASAFLHATWNLLSKRARGGAEFVWLFASLTVVIY
                     APFVVAYAVVYRPALTWTLVALAGVSSALHVLYFVFLQRGYRLGDLSLVYPTARGSGP
                     ALATVLAIVVLGERPGPQALLGTLLVVASVFVLAGGKGGGKRSRPAIGYGLLTGLFIG
                     IYTVFDGYAVGHAGAAPLVYTYLGEVGRAVLLLPFALRRPAVVRELWREHKREAIGIA
                     VLSPLAYILVLTAMQFTPVSLVAPAREVSILIATFFGLRLLAEGNVRRRALGAVGMV"
     gene            complement(903..1868)
                     /locus_tag="PKN52_04845"
     CDS             complement(903..1868)
                     /locus_tag="PKN52_04845"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HNQ99304.1"
                     /translation="MTTDNGSRAASTGAATPNGAGLIWFDGELVPEERAQVSVLTHAL
                     HYGTSVFEGIRAYEARRGAVVFRLEEHARRLLDSAKILGMRTNLTVEQVSAAVVDTLA
                     ANARKHAYIRPLIWYGSDALGVSPERNRLHFMVATLPWGTYLGDDVIRKGARLMTSSW
                     RRSPGDVLPTKAKAGGNYVNSVLANMEAKSNGFDEALLLDKQGFVAEGSGENVLLVKD
                     GVLQAIATSVNLRGITRDSVMRIAEAAGMRVETVMATRDELYTADEVFLVGTAAEVTP
                     VASIDRRAIGDGVAGPVALDLRERYLRVARGEVPEFERWLTYVDG"
     gene            2000..>2480
                     /locus_tag="PKN52_04850"
     CDS             2000..>2480
                     /locus_tag="PKN52_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNQ99305.1"
                     /translation="MNVLVTGGAGYIGSVTVATLLERGHSVVVLDDLSTGHRGAVPEG
                     VPFVHGSVADPDMVGYALAEHRVEAVVHFAAKSLVGESMSKPTKYFLANTASTFALLD
                     SAAAHGVGRFVLSSTAAVYGTPARVPIPETAELRPESVYGESKLMIERALKWLADTTG
                     "
BASE COUNT          383 a          897 c          854 g          346 t
ORIGIN      
        1 ccaccatgcc gacggcgccg agcgcgcgcc gcctgacgtt cccctccgcc agcagccgca
       61 gcccgaagaa cgtggcgatg aggatgctca cctcgcgcgc cggggcgacg aggctgacgg
      121 gcgtgaactg catggccgtg agcacgagga tgtaggcgag gggactgagg accgcgatcc
      181 cgatggcctc gcgcttgtgc tcccgccaca actcgcgcac gacggccggg cgccgcaggg
      241 cgaacggcag tagcaggacg gcgcgaccga cttcgcccag gtaggtgtac acgagcggcg
      301 ccgcccccgc gtgcccgacc gcgtagccgt cgaacaccgt gtagatgcct atgaagagcc
      361 cggtgagtag gccgtagccg atggccgggc gcgaccgctt gcctccgccc ttgcccccgg
      421 ccaggacgaa gacgctcgcc acgaccagca gggtgccgag cagcgcctgc ggtcccgggc
      481 gctcgccgag cacgacgatg gctagcaccg tcgccagcgc cggcccgctg ccccgcgccg
      541 tagggtagac gagcgagagg tcgcccagcc ggtaaccacg ctgcaggaac acgaagtaca
      601 gcacgtgcag cgcgctggag acgccggcga gcgcaacgag cgtccaagtg agcgccggcc
      661 tgtagacgac ggcgtacgcc acgacgaacg gcgcgtagat cacgaccgtg aggctcgcga
      721 acagccagac gaactccgcg ccgcccctgg cccgcttcga cagcaggttc caggtcgcat
      781 gcaggaacgc cgaggcgaag acgagggtga gtgccagggt cgtcacgcct cgccaccccg
      841 gcccgcccgg gcgaaccgca tgccctcagc gacccgccgc gttgcctgag ctgccggtgc
      901 cctcaaccgt cgacgtacgt caaccagcgc tcgaactctg ggacctcgcc gcgcgccacc
      961 cgcaggtagc gctcgcggag gtcgagcgcg acggggccgg cgaccccgtc gccgatggcg
     1021 cgcctgtcga tgctggccac gggcgtcacc tcggcggccg ttccgaccag gaagacctcg
     1081 tccgccgtgt agagctcgtc gcgcgtcgcc atcacggtct cgacgcgcat gccggccgcc
     1141 tcggcgatcc gcatgacgct gtcgcgcgtg atgccgcgca ggttcacgga cgtggcgatg
     1201 gcctgcagca cgccgtcctt gacgagcagg acgttctcgc cggagccttc ggccacgaac
     1261 ccttgcttgt cgaggagcag cgcctcgtcg aaaccgttcg acttcgcctc catgttggcc
     1321 agcacggagt tgacgtagtt gccgccagcc ttggccttgg tagggagcac gtccccgggg
     1381 gagcggcgcc aggagctggt catgaggcgc gcgcccttcc tgatcacgtc gtcgcccagg
     1441 taagtgcccc acggcagggt ggcgaccatg aagtgcagac ggttgcgctc cgggctcacg
     1501 ccgagcgcgt ccgagccgta ccagatgagc ggccggatgt aggcgtgctt gcgggcgttc
     1561 gccgccagcg tgtcgaccac ggcggccgac acctgctcga cggtcaggtt cgttcgcatg
     1621 ccaaggatct tcgcggagtc gaggaggcgc cgcgcatgct cctcgagccg gaagacgacc
     1681 gcaccgcgcc gcgcctcgta cgcccttatc ccctcgaaga cgctcgttcc gtaatgcaga
     1741 gcgtgcgtca ggacgctcac ttgcgcccgc tcctcgggta cgagctcgcc gtcgaaccag
     1801 atgaggcccg cgccgttggg tgtcgctgcc ccggtggagg cggctcggga gccgttgtcc
     1861 gtggtcatgt tcgctgccct ccttagaccc ccaagtatcg ctcaacgcgg gccgggcggt
     1921 ggcggcccgc gccgggtcgc gaaggccatg cccaggccgt gcaggcgtcg gtcgggggtg
     1981 gcaacggtag actcgcggca tgaacgtcct agtcaccggc ggcgccggct acatcggctc
     2041 ggtcactgtc gccacgctgc tcgagcgggg tcactcggtc gtcgtgctcg acgacctctc
     2101 gacgggccac aggggcgccg tgccggaggg agtgccgttc gtccacggca gcgtcgccga
     2161 cccggacatg gtgggttacg cgctcgccga gcaccgggtg gaggccgtcg tccatttcgc
     2221 ggccaagtcg ctcgtcggtg agagcatgag caagccgacc aagtacttcc tcgccaacac
     2281 ggcctcgacc ttcgcgctcc tcgactcggc cgcagcgcat ggcgttggcc gcttcgtgct
     2341 ctcctcaaca gccgccgtgt acggcacgcc ggcgcgcgtc cccatcccag agacggccga
     2401 actgcgcccc gagagcgtct acggcgagtc caagctcatg atcgagcgcg ccctcaagtg
     2461 gctggccgac acgaccggcc
//
LOCUS       DAOQYE010000239         2478 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_47031_length_2478_cov_2.170037, whole genome shotgun sequence.
ACCESSION   DAOQYE010000239 DAOQYE010000000
VERSION     DAOQYE010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47031_length_2478_cov_2.170037"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            172..1488
                     /locus_tag="PKN52_04855"
     CDS             172..1488
                     /locus_tag="PKN52_04855"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNQ99306.1"
                     /translation="MSTTNSSSARESYTLPGMLVTNRRFTVPLDHAKPNGERLGVFAR
                     ELAPLDRAGDDLPYLVFLQGGPGSPSPRPESRSGWIDRALQDHRVLLLDQRGTGLSSP
                     VSYQTVAERGSAEEQATYLTHFRADAIVADAELIRRELIGNERWTILGQSFGGFCALR
                     YLSAAPEGLDGVLITGGIPSLTRPADDVYRATFPRVEAKNRAFFARYPHAQLTCRRIA
                     DHLLKNDVSLPNGQRLTARQFQQIGLDFGGAGGFERVMDLIERAFVTVDGREELSYTF
                     LHGVFGATSFHTNPIFTVLHEAIYCQGTASNWAAQRVQAEFPQFDYAPGKEFLFTGEM
                     LYPWMLDEFETLRPLKGAAELLAAKSDWPALYDPGVLAKNRVPVAAALYYNDMYVDLG
                     LAMETVARVPNVEVWVTNEYEHNGLRMDGKRILDKLFAMLPHVERG"
     gene            complement(1493..>2478)
                     /locus_tag="PKN52_04860"
     CDS             complement(1493..>2478)
                     /locus_tag="PKN52_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proline racemase family protein"
                     /protein_id="HNQ99307.1"
                     /translation="TVDAHTGGEPLRVVTAGVPRIPGATILEKRRWVRDNLDHVRRAL
                     MWEPRGHADMYGCYVTEPVTAGADLGVIFMHNEGYSDMCGHGIIALATVAVAQGLVPR
                     TIGETRVGIDSPAGFIEAFVEWDGRRVGRVRFLNVPSFLYLRDLVVDTPGFGTLSVDI
                     AFGGAFYAYMDGDAVGLTVAPENARELIELGDQVKHAVMTTTTIQHPAVPELNRLYGT
                     IIAGPPRDPANHQANVCVFAEREVDRSPTGTGTSGRVAQLVARGRLGLGEELRNESIV
                     GSVFVGKAVSRTRVGDFDAVIPEVSGTAAILGFNQWVVEPGDTLGEGFLVR"
BASE COUNT          392 a          921 c          794 g          371 t
ORIGIN      
        1 cggccaacgg ccccgtgccg aggtcgggac ccccctccac cacgagccgc gtcggctcgc
       61 cgcccgtgtg cgagtcgacg aaccgcagcc gtctcacgcg ctcatgcgtt cgcgcttcgt
      121 tcacgccgca acctccaaca gtccgatacg ggcagttacc attccatcac catgtcgacg
      181 accaactcct cgtccgcccg cgagtcttac accctcccag ggatgctcgt cacgaaccgc
      241 cgcttcaccg tgccgctcga ccacgccaag ccgaacggcg agcgcctcgg cgtgttcgca
      301 cgcgaactgg caccgctcga ccgcgccggc gacgacctgc cctacctcgt gttcctccag
      361 ggcggacccg ggtcgccctc gccgagaccc gagtcgcgca gcggctggat agaccgtgcc
      421 ctgcaggacc accgtgtgct cctcctcgac cagcgcggca ctggcctctc ctcgcccgtg
      481 agttaccaga cggtggccga gcgtggtagc gccgaggaac aggccaccta cctcactcac
      541 ttcagggccg acgccatcgt ggcggacgcc gagctcatcc gccgcgagct catcggcaac
      601 gagcgctgga cgatcctcgg ccagagcttc ggcggcttct gcgcgctgcg ctacctctcg
      661 gccgcgccgg agggcctcga cggggtcctc atcacgggcg gcatcccgtc cctcacgcgc
      721 ccggccgacg acgtgtaccg cgctacgttc ccgcgcgtcg aagccaagaa ccgcgcgttc
      781 ttcgcacgct atccgcacgc ccagctgacg tgccggcgca tcgccgacca cctcctcaag
      841 aacgatgtga gcctcccgaa cgggcagcgc ctcacggcgc gacagttcca gcagatcggt
      901 ctcgacttcg gcggcgcggg cggcttcgag cgcgtgatgg acctcatcga gcgcgccttc
      961 gtcaccgtcg acggacggga ggagctctcc tacaccttcc tgcacggcgt cttcggcgcc
     1021 acgagcttcc acacgaaccc catcttcacc gtgctgcacg aggccatcta ctgccagggc
     1081 acggcctcca actgggcggc gcaacgtgta caggccgagt tcccgcagtt cgactacgcg
     1141 cctggtaagg agttcctctt cacgggcgag atgctctacc cctggatgct ggacgagttc
     1201 gagacgctgc ggccgctcaa gggcgcggcc gagctcctcg ccgccaagtc cgactggccc
     1261 gcgctctacg acccgggcgt gctcgccaag aaccgcgtac ccgtggccgc agcgctctac
     1321 tacaacgaca tgtacgtgga cctcggcctc gccatggaga cggtggcgcg cgtgcccaac
     1381 gtcgaggtat gggtcaccaa cgaatacgag cacaacggcc tgcgcatgga cgggaagcgc
     1441 atcctcgaca agctcttcgc catgctgccg cacgtggagc ggggttagag cctcaccgca
     1501 caaggaagcc ctcgccgagc gtgtcgcccg gctcgaccac ccactggttg aagccgagga
     1561 tggcggccgt gccgctcacc tcgggtatga cggcgtcgaa atcgccgacg cgcgtgcgcg
     1621 agacagcctt gcctacgaag accgagccga cgatgctctc gttgcgcagt tcctcgccga
     1681 ggccgagccg gccacgggca acgagctgcg caacgcgccc ggacgtgccc gtgcccgtcg
     1741 gcgagcggtc cacctcgcgc tccgcgaaca cgcacacgtt cgcctggtgg ttggcggggt
     1801 cccgcggcgg cccggcgatg atcgtgccgt acaggcggtt cagctccggc acggccgggt
     1861 gctggatggt cgtggtggtc atgacggcgt gcttcacctg atcgcccagc tcgatgagct
     1921 cgcgcgcgtt ctcgggcgcg acggtcaggc cgacggcgtc cccgtccatg tacgcgtaga
     1981 acgcgccccc gaacgcgatg tcgacgctca acgtgccgaa gccgggcgta tcgaccacca
     2041 ggtcccggag gtagaggaac gacggcacgt tcaggaagcg cacgcgtccg acgcgccggc
     2101 cgtcccactc gacgaacgcc tcgatgaagc cggccgggct gtcgatccca acgcgtgtct
     2161 cgccaatggt cctaggcacg agcccttgcg ccacggccac ggtggcgagg gcgatgatgc
     2221 cgtggccgca catgtccgag taaccctcat tgtgcatgaa gatgacgccc aggtcggcgc
     2281 cagcggtgac gggctcggtg acgtaacagc cgtacatgtc ggcgtgccca cgcggctccc
     2341 acatcagggc gcgcctcacg tggtcgaggt tgtcccgcac ccagcggcgc ttctccagga
     2401 tggtggcgcc ggggatgcgg gggacgccgg ccgtgacgac gcgcagcggc tcgccgccgg
     2461 tgtgcgcgtc tacggtgg
//
LOCUS       DAOQYE010000240         2476 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_47087_length_2476_cov_2.602644, whole genome shotgun sequence.
ACCESSION   DAOQYE010000240 DAOQYE010000000
VERSION     DAOQYE010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47087_length_2476_cov_2.602644"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="PKN52_04865"
     CDS             <1..505
                     /locus_tag="PKN52_04865"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNQ99308.1"
                     /translation="AVVPAAAPATPATPGAPDASGGAGADGTEVQTSAAPLPDGEPLY
                     VSTCSACHQATGMGIPMAFPPLAGHAPELYAADRDYPIEAVAFGLQGSINIHDTTYNG
                     VMPSHLHLQDADIAAILNYVMTAWGNEADLPGDFEPYTAADVEAVRGMMLMATEVHAN
                     RVAAGLD"
     gene            complement(598..>2476)
                     /locus_tag="PKN52_04870"
     CDS             complement(598..>2476)
                     /locus_tag="PKN52_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HNQ99309.1"
                     /translation="VQGGTGVDGYHQLLTEIIDNAIDEFLAGFANTVEVTLEPDGSAS
                     VADNGRGIPVDMMKREGRPAIEVIFTELHAGGKFDAGAYKVSGGLHGVGSSVVNALSN
                     WFVAEVRRDGKLYRIGFENGAVSEPLHVIGTAPAGVSGSTVTFHPDHGVFKEIEGFDY
                     SRVRRRLRELSYLTGGVRIVLTDNRGASPRTETFHEVGGVAAYATFLAREGTALYEKA
                     IAIKGTASVPTDSGTHDVEVDVGLIHTTGYGQTVLTYANMITNRDGGTHLTGFKSAYT
                     RALNNYAKAKNLIKKGEAAPTGDDFLEGIACVISVKLPDPQFESQAKVKLLNAEAQTA
                     VNSVVYEKLTEALEENPKVGRAIIDKAAQAARAREAARKARDLVRRANPLENDDLPGK
                     LADCQSQDPSVSEVYIVEGDSAGGTAKQGRERRFQAILPLRGKILNVEKANLGKILKN
                     AEIRAMIAAIGAGIEGTGDDAHFSIEDVRYHRIIIMTDADVDGSHIRTLLLTFFYRYM
                     RPIIDAGYLYIAQPPLYGLRIGRQKEMQYVYDEAALAQVQRQNRDKKLEVQRFKGLGE
                     MNAEQLWETTMNPETRILKRVTIDDVLEASETFEILMGTEVPPRREFIETNAHLAHLD
                     V"
BASE COUNT          343 a          822 c          859 g          452 t
ORIGIN      
        1 ggcggtcgtc ccggcggccg cgcccgcgac acccgccact cctggcgcgc cggacgcttc
       61 cgggggagcc ggtgccgacg gcacggaagt gcagacgagc gcagcccccc tgccggatgg
      121 cgagcccctc tacgtgagta cctgctcggc ctgccatcag gcgaccggca tgggcatccc
      181 catggccttc ccgccgctcg ccggtcacgc gcccgaactg tacgccgcgg accgcgacta
      241 cccgatcgag gccgtcgcct tcgggctcca gggcagcatc aacatccacg acacgaccta
      301 caacggcgtc atgccgtcgc acctgcatct gcaggacgcc gacatcgccg ccatcctgaa
      361 ctacgtcatg acggcctggg gcaacgaggc cgacctgccc ggcgacttcg agccgtacac
      421 ggccgccgac gtcgaggcgg tgcgcggcat gatgctcatg gccacggagg ttcacgccaa
      481 ccgcgtggcg gccggtctcg actgacttcg cctgcttgaa gcctcgctga tcgaagggcc
      541 cgcgctgttc tccgcgcggg cccttaccta tcgaacgtta ggctctgcga ccggcgttca
      601 gacgtccagg tgcgccaggt gcgcgttcgt ctcgatgaac tcgcggcgtg gcggaacctc
      661 ggtgcccatc aggatctcga aggtctcgct cgcctcgagg acgtcgtcga tggtgacgcg
      721 cttgaggatg cgcgtctccg ggttcatggt cgtctcccag agctgctcgg cgttcatctc
      781 gcctaggccc ttgaagcgct ggacctcgag cttcttgtcg cggttctggc gctggacctg
      841 ggcgagggcg gcctcgtcgt agacgtactg catctccttc tggcgtccga tgcgcaggcc
      901 gtagaggggc ggctgcgcga tgtacaggta gccggcatcg atgatggggc gcatgtagcg
      961 atagaagaac gtgaggagga gcgtccggat gtgcgagccg tcgacgtcgg cgtccgtcat
     1021 gatgatgatc cggtggtagc ggacgtcctc gatgctgaag tgggcgtcgt cgcccgtgcc
     1081 ctcgatgccg gccccgatgg ccgcgatcat ggcccggatc tcggcgttct tgaggatctt
     1141 gccgaggttg gccttctcga cgttgaggat cttgccgcgc agcggcagga tggcctggaa
     1201 gcggcgctcc ctgccctgct tggccgtgcc gccggccgaa tcgccctcga cgatgtacac
     1261 ctcgctcacg gacgggtcct gcgactggca gtcggccagc ttgccgggca ggtcgtcgtt
     1321 ctcgaggggg ttggcgcggc gcaccaggtc gcgcgccttg cgggcggcct ctcgggctct
     1381 ggcggcctgg gcggccttgt cgatgatggc cctgccgacc ttggggttct cctcgagcgc
     1441 ctcggtgagc ttctcgtaaa cgacgctgtt cacggccgtc tgcgcctcgg cgttgaggag
     1501 cttcaccttg gcctgcgact cgaactgcgg gtctgggagc ttgacggaga tgacgcaggc
     1561 gataccctcg aggaagtcgt cgcccgtcgg cgccgcctca cccttcttga tgaggttctt
     1621 ggccttggcg tagttgttca gcgcacgcgt gtatgcgctc ttgaagccag tcaggtgcgt
     1681 gccgccgtcg cggttcgtga tcatgttcgc gtaggtgagg accgtctggc cgtacccggt
     1741 ggtatggatg aggccgacat ccacctcgac gtcgtgcgtg ccggagtccg tcgggacgct
     1801 cgccgtgccc ttgatggcga tggccttctc gtagagcgcg gtcccctcgc gggcgaggaa
     1861 cgtggcgtag gccgccaccc caccgacctc atggaacgtc tcggtcctgg ggctggcacc
     1921 gcggttgtcg gtgaggacga tacgcacgcc gcccgtcagg taggagagct cgcgcaggcg
     1981 gcggcggaca cggctgtagt cgaacccttc gatctccttg aagacaccgt ggtcgggatg
     2041 gaacgtgacg gtgctgccgg agacccctgc cggtgcggtg ccgatgacgt gcagcggctc
     2101 agacaccgcg ccgttctcga agccgatgcg gtagagcttg ccgtcgcgcc gcacctccgc
     2161 gacgaaccag ttggagaggg cgttgacgac cgaactgccg accccgtgta ggccgcccga
     2221 taccttgtag gcgccagcgt cgaacttgcc gccggcgtgg agctcggtga agatgacctc
     2281 gatggccggg cgcccctcgc gcttcatcat gtcgaccggg atgccgcgcc cgttgtcggc
     2341 gacggaggcc gagccgtcgg gttcgagcgt gacctccacc gtgttcgcga agccggccag
     2401 gaactcgtcg atggcgttgt cgatgatctc ggtgagcagc tggtggtagc cgtcgacccc
     2461 ggtgccgccc tggacg
//
LOCUS       DAOQYE010000241         2475 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_47104_length_2475_cov_3.725207, whole genome shotgun sequence.
ACCESSION   DAOQYE010000241 DAOQYE010000000
VERSION     DAOQYE010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47104_length_2475_cov_3.725207"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..759
                     /locus_tag="PKN52_04875"
     CDS             <1..759
                     /locus_tag="PKN52_04875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99310.1"
                     /translation="LEQARNRARALGFSDQEMIQRLTSITQREDVPCHVVYVDRFAHI
                     ANKYAGRLEHHMGRAVRATPLTFDDITAESETAKAALTEAFYVVTVARNVPALEQRLP
                     RFARAHEILTIVSEPLPATVRALAALDPSKSTVVLAEEHYLYSSLNLLSLYSSIDPSD
                     AEAFTVDALDKFLAAACEADVILYTFGAGLVLAGLDLKAHGIRATTLELAFDIAPDSV
                     AKLRTVFGGVGSTAESVVGETRRRGVGSPLEAVL"
     gene            complement(837..2261)
                     /locus_tag="PKN52_04880"
     CDS             complement(837..2261)
                     /locus_tag="PKN52_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HNQ99311.1"
                     /translation="MSRRTVLGATLAAVLIAVVVTACTQGTPLKDAGVAFTSPADGTT
                     IVGSREVVVTLTLTNATNDAAIDVETTATVLSQTRSDTALEVKLELASNENTITVSVA
                     NPGQAVPGTATLALHYPFLSLTANQAAAVVIGQPDFDSASESDPTKEFGAPYGRPLVL
                     DGVLYLPDYGSNRVMGYLRVPDTNGVAADFVLGKQDFDDTASPVDAAGFKGPQTVAEY
                     QGKLYVIDYSFNRILVFDPPPVTSGAAAAHVIGQPDFYGSGAGTSSTRFRSPESMFIT
                     QDGKLLVADSGNDRVLIWNAVPTTVDTPADLVLGQPDMDSDVSAPTAANTMEYPVDVW
                     SDGERVVVVDPGNNRVLVWNTFPTEDFADADVVLGQADFVSSAPGSGAGDLSGPGSVY
                     SNGNQLFVADSDNNRVLVWNSFPSVSGQPADVAIGQEDLDSFVEGTSATGLTLPTGVY
                     VFGGDMFVTDKDNYRYLVYHGTNE"
BASE COUNT          405 a          800 c          826 g          444 t
ORIGIN      
        1 ttggagcagg cgcgcaaccg cgcgcgcgcg ctcggcttct ccgaccagga gatgatccag
       61 cgcctcacct cgataacgca gcgcgaagac gtcccttgcc acgtggtgta cgttgaccgc
      121 ttcgcccaca tcgccaacaa gtacgcaggg cgcctcgagc accatatggg ccgggcggtt
      181 cgggccacgc cgcttacgtt cgacgacata accgcggaga gcgagacggc caaggccgcg
      241 ctgaccgaag cgttctacgt cgtaaccgtc gccaggaacg ttccggcgct cgaacagcgt
      301 ctaccgcggt tcgcaagggc tcacgagatc ctgaccatcg tctccgaacc gctcccggcc
      361 accgtacgcg ctctggctgc cctcgacccc agcaagtcga ccgtcgtgct ggccgaggaa
      421 cactacctgt acagctcgct gaacctcttg tccctctaca gcagcatcga cccaagcgac
      481 gccgaagcgt tcaccgtcga cgccctggat aagttccttg ccgccgcttg cgaagcagac
      541 gtgatcctct acacgttcgg cgccgggctg gtgctggcgg gcctggacct gaaggcacac
      601 ggcatccgag cgaccacgtt ggagctagcc ttcgacatcg cacccgactc ggtggcgaag
      661 ctcaggaccg tcttcggcgg tgtcggctct actgccgagt ccgtcgtggg cgaaacgagg
      721 aggcgaggcg tgggtagccc cctggaggcc gtgctttagc cgcctgccgc ccgttgtcga
      781 cgatgagccc agggttcctc gtggaacgag caccacgctc ccgaggaacc tcctcttcac
      841 tcgttcgtgc cgtggtagac gaggtagcgg tagttgtcct tgtcggtgac gaacatgtca
      901 ccgccgaaga cgtacacgcc cgtcggtagg gtgagccccg tggcgctcgt gccctctacg
      961 aaactgtcga ggtcttcctg acctatcgcc acgtcggccg gttgtcccga gacgctgggg
     1021 aagctgttcc agacgagcac gcggttgttg tcgctgtctg ccacgaacag ttggttgccg
     1081 ttgctgtaga cgctgcccgg tccactgagg tcgcctgcgc cgctgccggg tgcgctcgaa
     1141 acgaaatccg cttggccgag cactacgtcg gcatcggcga agtcctcggt agggaacgtg
     1201 ttccagacga gcacgcggtt gttgcccggg tccacgacga cgacgcgctc cccatcgctc
     1261 cagacgtcga ccgggtactc catggtgttg gctgcggtgg gagcgcttac gtcagaatcc
     1321 atgtcgggct ggccgagcac gaggtccgcc ggcgtgtcga ccgtggtagg caccgcgttc
     1381 cagatcagca cacggtcgtt gcctgagtcc gcgacgagga gcttgccgtc ctgtgtgatg
     1441 aacatgctct ccggcgaacg gaaacgcgtg gaggacgttc ctgccccaga gccgtaaaag
     1501 tcaggttggc cgatgacgtg agcggccgcg gcgccgctcg tgacgggcgg gggatcgaac
     1561 accaggatcc ggttgaagct gtagtcgatg acgtacagct tgccttggta ctcagccacg
     1621 gtctgggggc ctttgaagcc ggcggcgtcg acaggcgagg ccgtgtcgtc gaagtcctgc
     1681 ttccctagta cgaagtcggc cgctactccg ttggtgtccg ggacgcgcaa gtaccccatg
     1741 acgcgattag agccgtagtc gggcaggtac agcactccgt cgaggacgag cgggcgcccg
     1801 tagggcgcgc cgaactcctt cgtcggatcg gactccgagg cggagtcgaa gtcgggttgg
     1861 ccgatgacga ctgccgcggc ctggttagca gtcagggaca ggaaggggta gtgcagtgcg
     1921 agcgtggccg ttccgggcac cgcttgcccg gggttggcga cggagacggt gatcgtgttc
     1981 tcgttgctcg ccagctccag cttcacctcg agggcggtgt cggaccgggt ttggctgagc
     2041 accgttgccg tggtctcgac gtcgatcgcg gcgtcgttgg tcgcgttggt cagggtcagg
     2101 gtgacgacga cctcgcggct gccgactatc gtcgttccgt cagcgggcga agtgaaggcg
     2161 accccggcgt ccttcaaggg tgtgccttgg gtgcaggccg tgacgaccac agctatgagc
     2221 accgcggcga gggtggctcc gagaacggtc ctacgagaca tcgatgggct ctcccttcgc
     2281 ccctgggcgg gcccgcaagc ccacgcatcg tcggagcggt tcgggagtca tcgtcaccta
     2341 ggcgcagtgc ggtgcgctgg gaattgccgc acaccgggca gcgggttcag cgcttcgact
     2401 ctccgctcgc cacgcccctg acatcgacca ccccgatcgc ctgcatgagc gcgtacatgg
     2461 tcgtcggacc cacga
//
LOCUS       DAOQYE010000242         2467 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_47360_length_2467_cov_3.349917, whole genome shotgun sequence.
ACCESSION   DAOQYE010000242 DAOQYE010000000
VERSION     DAOQYE010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2467
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47360_length_2467_cov_3.349917"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..889
                     /locus_tag="PKN52_04885"
     CDS             <1..889
                     /locus_tag="PKN52_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99312.1"
                     /translation="LVKSWSDVAHALDAQALVARALTMAGGIDGDDRAAREGDGGASA
                     ADPHDVRDLTVRVYDATASLQDATDDLPPYGFALVTFSSDDPLPVLGARSSGPSLTVE
                     SALAFEEGRYQGLDLGELYNNDAFSTPRNPSKGNFDARSSALGATYPAEHAPPASAVA
                     EILGVPFYFPPTDESANNVALKGQRVVVPTGRYTTLWLLGASEQGNYASDLTLEYADG
                     SAVSVTLGLSDWCQLPRYGERVALEFNQRRGAGGAVERITCRIFMQTIDLEPGKELTA
                     LLLPDRETMHVFAVTLEEP"
     gene            886..1797
                     /locus_tag="PKN52_04890"
     CDS             886..1797
                     /locus_tag="PKN52_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HNQ99313.1"
                     /translation="MKLVRHPANPVLKPEPSSPWEALNVFNPSVIFHGGLWHMHYRAQ
                     GVDYVSRIGYAVSTDGVHWNRLREPVLVPDGESEARGVEDPRVTELDGRFYMAYTAYG
                     RVGKHAETGVTPTGITPMYAVSDDLVRWERLGPVVLHEDNKDHALFPERIGGKYVTFH
                     RRPPSIWLAVSDDMRAWGDHREILGPRPGLWDGKRVGAGGPPIRTQHGWLILYHGYDE
                     AHVYCMGAAMLDLADPTRVLKRPKGTILEPQETWEIKGDVPNVVFGCANPVVDGVVHL
                     YYGGADRVIGLATAPLSELVEWVMLEG"
     gene            2215..>2467
                     /locus_tag="PKN52_04895"
     CDS             2215..>2467
                     /locus_tag="PKN52_04895"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNQ99314.1"
                     /translation="MTKLNVAIIGQNFMGKAHSNAWRNAPKFFDLPATPVMKVACATN
                     QESLQRFAGNWGWQETAADWREVVARDDIDVVDICTPTDL"
BASE COUNT          418 a          844 c          839 g          366 t
ORIGIN      
        1 cctcgtgaag agctggtcgg acgtggcgca cgctttggac gcgcaggccc tagtggctcg
       61 ggcgctgacg atggcgggcg gcatcgatgg agacgatcgg gctgctcgtg aaggggacgg
      121 cggggcgtcg gccgcggacc ctcacgacgt gcgcgacctg acggtccgcg tctacgacgc
      181 caccgcgagc ctccaggacg caacggatga cctgccgccc tacggtttcg ccctcgtgac
      241 cttctcgagc gacgacccgc tgcccgtgct cggcgcgcgc tccagcggcc cgtcactcac
      301 cgtcgagagc gccctcgcgt tcgaggaggg caggtaccaa ggcctcgacc tcggcgagct
      361 gtacaacaac gacgccttct cgacgccgcg caacccctcc aagggcaact tcgacgcgcg
      421 ctccagcgcc ctcggcgcca cctatcccgc cgagcacgcg ccaccggcca gcgccgtcgc
      481 cgagatcctg ggtgtgccct tctacttccc gccaacggac gagtcggcga acaacgtcgc
      541 cctcaagggc cagcgcgtgg tcgtcccgac agggcggtac acgaccttgt ggctgctggg
      601 cgcctctgag cagggcaact acgcgagcga cttgaccctc gagtacgcgg acgggagcgc
      661 ggtgtccgtc acgctcggcc tgtcagactg gtgccagctt ccgcgctacg gcgagcgggt
      721 ggcgctagag ttcaaccagc ggcgcggcgc gggcggggcg gtcgaacgca tcacgtgccg
      781 tatcttcatg cagaccatcg acctcgagcc cggcaaggag ctcaccgccc tcctcctacc
      841 ggaccgggag accatgcacg tgttcgccgt cacgctggag gagccttgaa gctcgttcgc
      901 caccccgcca atcccgtcct caagcccgag ccgtccagcc cgtgggaggc gctcaacgtg
      961 ttcaatccgt ccgtgatctt tcacggcgga ctgtggcaca tgcactaccg cgcgcagggt
     1021 gtcgactacg tgtcgcgcat cggctacgcc gtctccaccg acggcgtcca ctggaaccgt
     1081 ctacgcgagc cggtgctggt acctgacggg gagagcgagg ccaggggcgt cgaggacccg
     1141 cgggtgacgg agctggacgg taggttctac atggcctaca cggcctacgg gcgggtcggc
     1201 aagcacgccg agacgggggt gacgcccacg ggcatcaccc ccatgtacgc cgtctccgac
     1261 gacctcgtca ggtgggagcg cctcggcccc gtcgtactgc atgaggacaa caaggaccac
     1321 gcgctcttcc cggagcgtat cggtggcaag tacgtgacgt tccatcgtcg gccgccgtcc
     1381 atctggctcg cggtcagcga cgacatgcgc gcatggggcg atcaccgcga gatcctcggt
     1441 ccgaggccgg gcctctggga cggcaagcgt gtgggggccg gcggcccgcc catccgtacc
     1501 cagcacggct ggctgatcct gtatcacggc tacgacgagg cccacgtgta ctgcatgggc
     1561 gccgccatgc tcgacctcgc tgacccgacc agggtcctga agcgcccgaa gggcacgatc
     1621 ctagagccgc aggagacgtg ggagatcaag ggcgacgtgc ccaacgtcgt gttcggctgt
     1681 gcgaacccgg tggtggacgg ggtcgtgcac ctctactacg gcggggcgga ccgggtcata
     1741 gggctcgcga ccgcgccgct cagcgagctg gttgagtggg tgatgttgga ggggtgactc
     1801 gcgcgcagta gtggtgccat gagaggccgc cgaccacccc gagcggagcc cggcgatgca
     1861 tcttcagttg gtgatggcga tccaagcgca ctaccatgat cgcacgatgt cgcctcgatc
     1921 agtgcccgtg cgcttcgcat ctctccgcga ccgcgtgtac gccttggggc tatccggacg
     1981 ggttggaacg cagccagaac gtcgaccccg gcccaggaag gaaccccggc cctggaagga
     2041 accccggccc tggaaggaac tcggggacag cggctacacc ggctttctgg cgatggggac
     2101 ggacatccgg ctacagcggc gacgggcacg cggccatccg caagagcgtc gcgaacctca
     2161 agcgcagcgc cggagaactg cgttgaggct taaaggagca aaggagcagc gcgcgtgacc
     2221 aagctgaacg tcgccatcat cgggcagaac ttcatgggga aggcccactc gaacgcctgg
     2281 cgcaacgcgc ccaagttctt cgacctgccc gccacccctg tgatgaaggt ggcgtgcgcc
     2341 accaaccagg aatccttgca gcgcttcgct ggcaactggg gttggcagga gacggcggcg
     2401 gactggcggg aggtggtggc gcgcgacgat atcgacgtcg tcgacatctg cacaccgacc
     2461 gacctcc
//
LOCUS       DAOQYE010000243         2464 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_47439_length_2464_cov_3.508510, whole genome shotgun sequence.
ACCESSION   DAOQYE010000243 DAOQYE010000000
VERSION     DAOQYE010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47439_length_2464_cov_3.508510"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="PKN52_04900"
     CDS             <1..510
                     /locus_tag="PKN52_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNQ99315.1"
                     /translation="EFTAAVGRVPATRTVFNNTCLDILAVAAKMLDGEVLYRAREYDA
                     AFAALREAIALSDALPYDEPWAWMQPVRHALGALLLERGRVEEALEAYRDDLGLSGRV
                     PRALQHPDNVWALHGLVECLERLGRPEEAEPFRQRLAVAQARADVVIGSSCFCRLEER
                     KPDSGHCCH"
     gene            complement(632..1585)
                     /locus_tag="PKN52_04905"
     CDS             complement(632..1585)
                     /locus_tag="PKN52_04905"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNQ99316.1"
                     /translation="MTLSNLTRQGIVLRIVSTVFFAGMAVFVKLASEEAPVGQIVFFR
                     SAFAMIPLVIFLLARREFPAGLATKRPFGHLARSGLGAASMFLSFAAISRLPLADSTL
                     VSYVTPLLTALLGGLLLGEVLTRSKMTGIFLGMCGVLVLTLPQLIGTTIDATRLVGYG
                     LGLLSAVLAAGAMIQVRRLGTTESPGAIALYFVLVSALASLFTLPTGWVAPSTGTLAM
                     LVLAGLVGGLAHIAMTLSYRYAEASTLAPFEYLSLIWAGVLDLTVFQLGLSPWFLLAV
                     PLLITGAAVSTGTLGEMLSRLTKRNVRRRLPGEPPTRKRAA"
     gene            1867..>2464
                     /locus_tag="PKN52_04910"
     CDS             1867..>2464
                     /locus_tag="PKN52_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99317.1"
                     /translation="MSRETKDRRPWGRRLSRLALTLAMALWFVAGIGLAQQGSEAQGA
                     AGHEDDGHDHADHDHPVTGADEHAHADDEHADHDHHDHEHGGAGLAGIRLLLGEAGSP
                     SAYVVSAENGEVVGSFTVPGVSRVEQLADTRYGALVHTDANRVTFVYSGLSAVDHGDH
                     MDLLIGNPYVAATINTGRNPLHFASYGNGIAIFNEADGT"
BASE COUNT          421 a          752 c          931 g          360 t
ORIGIN      
        1 gagttcacgg ccgccgtggg gcgcgtgccg gccacccgga cggtgttcaa caacacgtgc
       61 ctcgacatcc tcgccgtggc cgcgaagatg ctggacggcg aggtcttgta ccgcgcccgc
      121 gagtacgacg ccgccttcgc tgcgctccgt gaggccatcg cgctctcgga cgccctgccg
      181 tacgacgagc cgtgggcctg gatgcagccc gtgcggcacg ccctgggggc gttgctgctg
      241 gaacgcggcc gggtcgagga ggcgctcgag gcgtaccggg acgatctcgg cctcagcggt
      301 cgcgtcccac gcgcgttgca gcaccccgac aacgtgtggg cgctgcacgg actcgtggag
      361 tgcctcgagc gcctcgggcg gcccgaggag gcggagccgt tcagacagcg gctggcggtg
      421 gcgcaggcgc gtgccgacgt ggtcatcggt tcatcttgct tctgcaggct ggaggagagg
      481 aagccggact cggggcactg ctgccactga ctggtcgcgg caggtttcct gatcccttag
      541 gacttgccgt tgacccggtc gcccggggct gcggtcgcga cggtggggtg gcgacgatgg
      601 attaccggag ctgtcgttat gcccggctac cctacgccgc ccgcttgcgt gtgggcggct
      661 ctccgggtag tcggcgcctc acgttccgct tcgtgagcct cgacagcatc tcgcctagag
      721 tgccggtgga gacggcagcg cccgtgatca ggagcggcac cgcgaggagg aaccacggac
      781 tcaggccgag ctggaagacg gtgaggtcga ggaccccggc ccagatcagc gagaggtact
      841 cgaagggggc tagggtcgag gcctcggcgt agcggtacga cagcgtcatg gcgatgtgcg
      901 ccagtccgcc gaccagtccc gcgagcacca gcatggccag ggtgcctgtg ctgggcgcga
      961 cccaaccggt cggtagcgtg aagagcgagg cgagtgccga gacgagtacg aagtagaggg
     1021 cgatggcgcc cggcgattcg gtcgtgccga ggcgcctcac ctggatcatc gcgccggcgg
     1081 cgaggaccgc ggagagtagg ccaaggccgt agccgacgag gcgcgtggcg tcgatcgtcg
     1141 tgccgatgag ctgcgggagc gtcagcacga gcaccccgca catgcctagg aagatgccgg
     1201 tcatcttgga gcgcgtcagg acttcgccga gcaggagccc gccgaggagg gcggtgagga
     1261 gcggcgtgac gtagctgacg agcgtcgagt cggcgagcgg tagccgcgag atcgccgcga
     1321 acgagaggaa catgctggcg gcgcccagcc cggacctggc gaggtggccg aaggggcgct
     1381 tggtggccag tccggcaggg aactctctgc gcgcgagcag gaagatcacg agtgggatca
     1441 tggcgaacgc cgagcggaag aagacgatct gaccgacggg cgcctcttcg ctcgcgagct
     1501 tcacgaacac ggccatgccg gcgaagaaga cggtggagac gatgcgcagg acgatgcctt
     1561 ggcgcgtcaa gttgctgagg gtcatggtcg gttcggcgtg aacacgaggt tgaggcggtg
     1621 gccgcgcacg aaccagatac tacgcggcgg aacgcccggg atgtgcgtga ccggaccgct
     1681 cagcggagtc cagggtcgga caccgccttg ccgggctcct ggcggggatc ttagggggca
     1741 gatgtgcgtc ggctcgaggt cgtttggggg taggtgttgc gctctcgagg agggcatgca
     1801 tatactccga ttctcggtct tgcgaatgag tcgcaattgc atgatagacc gaacttggag
     1861 gatgcgatga gcagggagac gaaggacagg cggccgtggg gccggcggct gtcgcgacta
     1921 gcgctgactc tggcgatggc gttgtggttc gtggccggga tcggtctggc gcagcagggt
     1981 agcgaggcgc aaggagcggc cggccacgag gacgacggtc atgaccatgc cgatcacgac
     2041 caccccgtca caggagccga tgaacacgcg cacgccgatg acgagcatgc cgatcacgac
     2101 caccacgacc atgaacatgg gggtgccggc ctggccggaa tcaggctcct gctcggggag
     2161 gccggcagcc ccagcgcgta cgtggtaagc gccgagaacg gggaggtcgt aggcagcttc
     2221 acggtcccag gcgtgagccg cgtcgagcag ctggccgaca cgcggtacgg cgcgctcgtc
     2281 catacggacg cgaaccgcgt gaccttcgtg tactcgggcc tctcggccgt ggaccacggc
     2341 gaccacatgg acctgctgat cggcaacccg tacgttgcgg ccactatcaa cacgggcagg
     2401 aacccgctgc acttcgcgag ctacgggaac ggcatcgcca tcttcaacga ggccgacggc
     2461 accg
//
LOCUS       DAOQYE010000244         2464 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_47450_length_2464_cov_2.544624, whole genome shotgun sequence.
ACCESSION   DAOQYE010000244 DAOQYE010000000
VERSION     DAOQYE010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47450_length_2464_cov_2.544624"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="PKN52_04915"
     CDS             <1..267
                     /locus_tag="PKN52_04915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99318.1"
                     /translation="VDKELAPRLSFLMYLVVSLGVAVLGARDVLEAGVELAPLVTMFV
                     ASFAVGYGALLLVFTVLKRGRFRVFSPYLWAVSAFTLAYLALAR"
     gene            425..2002
                     /gene="gltX"
                     /locus_tag="PKN52_04920"
     CDS             425..2002
                     /gene="gltX"
                     /locus_tag="PKN52_04920"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HNQ99319.1"
                     /translation="MEVVTRIAPSPTGDPHVGTAYIGLFNYAFARRHGGRFIFRLEDT
                     DRQRYQAGAEARILEMFSWLGLEPNESPAAGGPNGPYRQSERLAVYHAHVEELLASGR
                     AYRAFETAEEIEAMRLEQKRLNKPIGYDGRGRGMPEAEQTRRHAAGEPNVVRLVTPDE
                     GSTTFRDRLRGDVTIPNSEIRDPVLMKSDGYPTYHLANVVDDHLMGVTHVIRAEEWVT
                     STPIHVLLYQAFDWALPEFIHMPLLRNPDRSKVSKRKLDTSVDSYRQQGFLPEALLNF
                     LANMGWSMPDGREYFSVADMIAEFDIDRVSLGGPVFDLKKLRSFNAKYLRDLLSLEDV
                     AARVRPLLEDEGYDCSDEDYLLDVVDVLRPRAETLVELVGQAGYFFRDTLRYDESARK
                     QLASGQSFLQDVERELSMLEFFDYDGVDDMLRDYVQEQAVPMGKVLMPLRAALTGTTN
                     APGVTDLIVILGKREALRRIGQALTFIDAGLPDDDPQRLLEEEKARKAAAEEARKSRV
                     KSVGAPPTAAAGGKGSR"
     gene            1999..>2464
                     /locus_tag="PKN52_04925"
     CDS             1999..>2464
                     /locus_tag="PKN52_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HNQ99320.1"
                     /translation="MSWFDRLREGLNRTRTATFGGGRGEAWEMDWDDLEFALIGADVG
                     AKLAATVVTEAKEERQRGTPFREALEKALLDQLEPDRMRQKLRRVGFNLDVSRNVIEP
                     TGHVVMVVGVNGVGKTTTIAKLGHYYLQHGKSVMFAAGDTFRAAGSAQLAVWG"
BASE COUNT          407 a          861 c          828 g          368 t
ORIGIN      
        1 gtggacaagg agctggcccc ccgcctctcg ttcctcatgt acctcgtcgt ctcgctcggc
       61 gtcgccgtcc tcggggcgag ggacgtgctg gaggccgggg tcgagctggc gcccctcgtc
      121 accatgttcg tggcgtcgtt cgccgtcggc tacggcgccc tgctcctcgt gttcacggtg
      181 ctcaagcggg ggcggttcag ggtgttctct ccctacctgt gggccgtgtc ggcattcacc
      241 ctcgcctacc ttgccctcgc gcgctagcgc gcgtgccgca tgcgcgctag ccgcgcgtgc
      301 ccggggccga ccagcgtgac aggcaaggcg ccggacgcgc ttgaaaaaca ccgcgtagga
      361 cgcgcatgcg taacgcgagc gcctacgcgc cctgggctat gatgccgaga taggaggcgc
      421 ctccatggaa gtcgtcacca ggatcgcacc gtcgccgacc ggagaccctc acgtcggcac
      481 ggcctacata ggactgttca actacgcgtt cgcgcggcgg cacggtgggc gcttcatctt
      541 ccgcctcgaa gacaccgacc ggcagcgcta ccaggctgga gccgaggccc gcatcctcga
      601 gatgttctcc tggctcgggc tggagccgaa cgagagcccg gccgccgggg ggccgaacgg
      661 cccgtaccgc cagagcgagc gcctcgccgt ctatcacgcg cacgtcgagg agctgctcgc
      721 ctccggtcgc gcttaccgcg ccttcgagac cgcggaggag atcgaggcca tgcgcctcga
      781 gcagaagcgc ctcaacaagc cgatcggcta cgacggccgc gggcggggca tgccggaggc
      841 cgaacagacg cgcaggcatg ccgccgggga gccgaacgtc gtgcggctcg tcacgcccga
      901 cgagggctcc acgaccttca gggaccgcct ccgcggcgac gtcacgatcc ccaactccga
      961 gatccgcgac cccgtcctga tgaagagcga cggttacccc acctaccacc tggccaacgt
     1021 cgtggacgac cacctgatgg gcgtgaccca cgtcatccgc gcggaggagt gggtgacgag
     1081 cacgcccatc cacgtgctgc tctaccaggc gttcgactgg gcgctccccg agttcatcca
     1141 catgcccctc ttgaggaacc ccgaccgaag caaggtcagc aagcgcaagc tcgacacgtc
     1201 cgtcgactcg tacaggcagc agggcttcct gccggaggcg ctcctcaact tcctcgccaa
     1261 catgggctgg agcatgccgg acgggcgcga gtacttcagt gtcgccgaca tgatcgccga
     1321 gttcgacatc gacagggtct cgctcggtgg gccggtcttc gacctcaaga agctacgcag
     1381 cttcaacgcg aagtacttgc gcgacctcct ctcgctcgag gacgtcgccg cgcgcgtcag
     1441 gcccctcctc gaggacgagg gttacgactg ctcggacgag gactacctgc tcgacgtcgt
     1501 cgacgtcctg cggccgcgtg ccgagacgct cgtcgagctc gtcgggcagg ccggttactt
     1561 cttcagggac acgctccgct acgacgagtc tgcccgcaag cagctcgcct ccggacagtc
     1621 gttcctgcag gacgtcgagc gcgagctctc catgctcgag ttcttcgact acgacggcgt
     1681 cgatgacatg ctcagggact acgtgcagga gcaggcggtg ccgatgggca aggtcctcat
     1741 gccgctgcgc gccgccctga caggcacgac caacgcgccc ggcgtcactg atctcatcgt
     1801 catcctcggc aagcgcgagg cgctcagacg catcggccag gccctaacgt tcatcgacgc
     1861 gggcctgcct gacgacgacc cgcagcggct gctcgaggag gagaaggcgc gcaaggcggc
     1921 ggccgaggag gcccgcaaga gccgtgtcaa gagcgtgggc gcgccgccta cggcggccgc
     1981 ggggggcaaa gggagcagat gagctggttc gacaggctta gggaagggct gaaccgcacg
     2041 cgcaccgcca ccttcggcgg cgggcgcggt gaggcctggg agatggactg ggacgacctc
     2101 gagttcgccc tcatcggcgc ggatgtcggc gccaagctcg ctgccaccgt ggtgaccgag
     2161 gcgaaggagg agcgccagcg cggcacgccg ttccgggagg ctctcgagaa ggcgctcctc
     2221 gaccagctgg agcccgatcg gatgcgtcag aagctgcggc gcgtcggctt caacctcgac
     2281 gtctcgcgca acgtcatcga gccaacgggc cacgtcgtga tggtcgtcgg ggtcaacggc
     2341 gtcggcaaga ccaccaccat cgccaaactg ggccactact accttcagca cggcaagagc
     2401 gtgatgttcg cggccggcga caccttccgc gcggcgggca gcgcgcagct cgcggtgtgg
     2461 ggtg
//
LOCUS       DAOQYE010000245         2451 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_47836_length_2451_cov_2.616861, whole genome shotgun sequence.
ACCESSION   DAOQYE010000245 DAOQYE010000000
VERSION     DAOQYE010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47836_length_2451_cov_2.616861"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="PKN52_04930"
     CDS             <1..188
                     /locus_tag="PKN52_04930"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HNQ99321.1"
                     /translation="VLRLILRGLPNKLICRQLDLAEGTVMVHVSAVLRALGVRNRTQA
                     VIAASRMGLRIKLDDSP"
     gene            268..465
                     /locus_tag="PKN52_04935"
     CDS             268..465
                     /locus_tag="PKN52_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HNQ99322.1"
                     /translation="MNQVFQVEGMSCGHCVHAVTGAIQEIDAAARVQVDLATGTVEVV
                     SERPRRELAEAIREAGYDIAA"
     gene            437..1744
                     /locus_tag="PKN52_04940"
     CDS             437..1744
                     /locus_tag="PKN52_04940"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HNQ99323.1"
                     /translation="MKPATISRPEPAGPLNIGQAARATGVVLGMELLALGCNMNFAPV
                     LDLATRPESDVMGVRVFGSDPDLAGRLGAQLIVGMQSTGVLATAKHFPGHGDTRQDSH
                     HGAPVIDAPLATLLRRELAPFRAAFAAGVAAVMTCHAYYPALDPDAVATHSRAVLVDL
                     LRGELDFEGLIITDALDMHAFGAMSGVERARRSVAGGADLALLGHLAGQAEIVESLLG
                     AADAVSSARVMAVRERLPRELPPLKVVGSELHREVARATSEAAVTAVRGEPRLASLAS
                     VLVVSVGAGDLTPAETTSGQELGLADALRADVAEVIEVRFARGAGASEIDAVVARVAQ
                     WRGARAAAGEPGEVVVATVNAAGDEAQLALLRRLVALGADPVLVAMRSPLDVAVADHV
                     GRAICTYGRRTPQAEAAARVLCGRIQATGALPVALPEGVGARA"
     gene            1837..>2451
                     /locus_tag="PKN52_04945"
     CDS             1837..>2451
                     /locus_tag="PKN52_04945"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HNQ99324.1"
                     /translation="MLALVIGSLATARAQGASWCVAVWYPSSDQPGGFQTILANADVV
                     DMVFPFWYTPDADGAILSRAGADWREQVAAWREAGLVVMPSVFATHSAFLNEPRRSAH
                     VAALLALVDENDYDGLDLDYEEFPLATREPFSEFVEALGAGLHARGKLLSVTVHAKTE
                     AEPASYQSAAAQDWPRLAAVADVFNVMTYDYTNRNEPPGPVAPRS"
BASE COUNT          331 a          882 c          876 g          362 t
ORIGIN      
        1 acgtcctgcg cctgatcctg cgcggcctgc ccaacaagct gatctgccgc cagctcgacc
       61 tcgcagaggg cacggtcatg gtgcacgtca gcgccgtgct tcgcgcgctg ggcgtgcgca
      121 accgcaccca ggcggtgatc gccgccagcc ggatgggact gcgaatcaag ctcgacgaca
      181 gcccttgacc ttcccataat gggaagctcg acactggctc cgatcgaggc atggatcgcg
      241 agcgcgatca cacacacgga gatgaacatg aaccaggtat tccaggtcga gggcatgagc
      301 tgcggacact gcgtgcacgc ggtcaccggc gcgatccagg agatcgacgc tgccgccagg
      361 gtccaggtcg acctcgccac cggcacggtc gaggtggtga gcgagcgacc ccgccgcgaa
      421 ctggccgagg cgatccgtga agccggctac gatatcgcgg cctgagcccg ccgggccgct
      481 gaacatcggc caggccgctc gcgccaccgg cgtggtgctc gggatggagc tcctcgcgct
      541 gggctgcaac atgaacttcg cgccggtgct ggacctcgcc acccgcccgg agagcgacgt
      601 catgggggtg cgggtgttcg gctccgaccc cgacctggcg ggccggctcg gggctcagct
      661 catcgtgggc atgcagtcga ccggcgtgct ggccaccgcc aagcacttcc ccggccacgg
      721 cgacacccgc caggacagtc accacggcgc ccccgtgatc gacgcgccgc tcgccaccct
      781 gctgaggcgc gaactggcgc cgttccgcgc ggctttcgcc gccggcgtgg ccgccgtcat
      841 gacctgccat gcctactacc cggcgctcga ccctgacgcg gtggccaccc actcgcgcgc
      901 ggtgctcgtc gacctgctcc gaggcgaact cgatttcgag ggtctgatca tcaccgacgc
      961 ccttgacatg cacgcgttcg gcgccatgtc gggcgtggag cgtgcgcgcc gttctgtcgc
     1021 cgggggcgct gacctcgccc tcctcggaca cctggcaggt caggccgaga tcgtcgagag
     1081 cctcctcggc gcggccgacg ccgtgagctc ggctcgcgtc atggcggtcc gcgagcgcct
     1141 gccgcgcgag ctgccgcctc tcaaggtcgt gggctcggaa ctccaccgcg aggtggcccg
     1201 ggctacttcc gaggcggccg ttacggccgt gcgcggcgag ccgcgcctcg cgtccctcgc
     1261 ctctgtcttg gtcgtgagcg taggtgcggg cgatctcacc cccgcagaga cgacctccgg
     1321 gcaggagctt gggctggcgg acgcgttacg cgcggacgtc gcggaggtca tagaggtgcg
     1381 cttcgcacgc ggggctgggg cgagcgagat cgacgcggtc gtggcgcgcg tggcgcagtg
     1441 gcgcggtgcc cgcgccgccg ccggcgaacc gggcgaggtc gtcgtcgcca ccgtcaacgc
     1501 cgccggcgac gaggcgcagc ttgcgctcct gcggcggctc gtcgcgctcg gagccgaccc
     1561 cgtgctcgtc gccatgcgta gcccgctaga tgtcgcggtc gccgatcatg tcggtcgcgc
     1621 catctgcacc tacggccgcc gcaccccgca ggccgaggcc gcggcacgcg tgctgtgcgg
     1681 ccggatccag gccacgggcg ccctgccggt agcgttgccg gagggcgtgg gagcgcgggc
     1741 gtgaggctgg cggctccggc gcgcgcgaac cgggttgggc tcgcgcttgc gcgtgcgaag
     1801 agcgccgggc tcgcgcgtgc gaagcgtgcc ggattggtgc tcgccctggt catcgggtca
     1861 ttggcgacgg ccagggcgca gggtgcgagc tggtgcgtcg ccgtctggta cccgtcgtcc
     1921 gatcagccgg gcggttttca gacgatccta gccaacgcgg acgtcgtcga catggtcttc
     1981 ccgttctggt acacgcccga tgccgacggc gccatcctgt cgcgcgccgg cgccgactgg
     2041 cgcgagcagg tggcggcttg gcgggaggct gggctggtcg tcatgccgag cgtgttcgcc
     2101 acccactccg cgttcctgaa cgagccgcgt aggagcgccc acgtggcggc gctcttggcg
     2161 ctcgtcgacg agaacgacta cgacggcctc gacctcgact acgaggagtt cccgctcgcc
     2221 acgcgggagc ctttcagcga gttcgtcgag gccctgggcg ccgggctcca cgcgcggggg
     2281 aagctcctct ccgtcacggt ccacgccaag acggaggccg agccggcgag ctatcagagc
     2341 gcggcggcac aggattggcc acgcctggca gcggtcgcag acgtcttcaa cgtcatgacc
     2401 tacgactaca cgaaccgcaa cgagccgccc ggacccgtcg cgccccgttc g
//
LOCUS       DAOQYE010000246         2444 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_48082_length_2444_cov_2.394307, whole genome shotgun sequence.
ACCESSION   DAOQYE010000246 DAOQYE010000000
VERSION     DAOQYE010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48082_length_2444_cov_2.394307"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..681)
                     /locus_tag="PKN52_04950"
     CDS             complement(<1..681)
                     /locus_tag="PKN52_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HNQ99325.1"
                     /translation="MDERVTAANGAIADFRNARRRADLAGVMALLSGQSDRLLRYDEV
                     RKRLKGVEASTSTLQDIPLDAIVGSVGRYHDFNRSFLPLTDSDRSRWVGVKLAMLGME
                     GVPPIDVYRIGEAYFVKDGNHRVSVARQLGASHINAWVTPVVSRVAFTPDMDMDDLIR
                     ASEFADFLDATHLDELRPEADLTVTVPGQYPKLLEHIQVHQYYMGIDEDRPVPWLEAV
                     EHFYDTVYL"
     gene            715..1458
                     /locus_tag="PKN52_04955"
     CDS             715..1458
                     /locus_tag="PKN52_04955"
                     /inference="COORDINATES: protein motif:HMM:NF013488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HNQ99326.1"
                     /translation="MSGARRRLRLLSGMKVEIWSDIACPWCYIGKTRFEKALSAYEHA
                     GEVEVVWRSFELQPDASRQSPGTTATHLMDKYGRSHDEVLGMMERVSEVAASEGLEFH
                     LERGVAANTFDAHRLVHYGASVGKGEAVMRRLMRAYQSDGEDLSDHGTLARLAVEAGL
                     DASGARDVLAGDAFSDAVRSDERRARSLGVDGVPFFVFDEVHGVSGAQPTEFFLSALR
                     QLGPQVRPLTMHGSAAPVCDEDGCEVPQG"
     gene            1800..>2444
                     /locus_tag="PKN52_04960"
     CDS             1800..>2444
                     /locus_tag="PKN52_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNQ99327.1"
                     /translation="MPDTVVDLGAQSEQSPSQAPALRDRGTYYATTPIFYVNAAPHIG
                     HAYTTILVDVVTRYHRLKGDDTFFLTGTDEHGEKIQKAAEAAGESPQAYTDQVSGEFR
                     AAWDRLGIRYDDFIRTTEPRHKKVVQEVLQRVYDNGDIVFGEYAGLYCVKCERYYTDK
                     ELVDGKCPQHEIEPEYRTEANYFFRMEKYRLWLRDLLTDQPDLIRPERYRNEVMA"
BASE COUNT          384 a          864 c          836 g          360 t
ORIGIN      
        1 caggtacacg gtgtcgtaga agtgctccac ggcctcgagc cacggcaccg gccggtcctc
       61 gtcgatgccc atgtagtact ggtgcacctg gatgtgctct aggagcttcg ggtactggcc
      121 agggaccgtg accgtgaggt cggcctcggg gcgcagctcg tcgaggtggg tcgcgtcgag
      181 gaagtcggcg aactcgctcg cgcggatgag gtcgtccatg tccatgtcgg gcgtgaacgc
      241 cacgcgcgac acgacgggcg tcacccaggc gttgatgtgg ctcgcgccca gctgccgcgc
      301 cacggagaca cggtggttgc cgtccttgac gaagtacgcc tccccgatgc ggtagacgtc
      361 gatcggcggc acaccctcca taccgagcat ggcgagcttg acgcccaccc agcgggagcg
      421 atccgagtcg gtcagaggca ggaacgagcg gttgaagtcg tggtagcggc cgacgctgcc
      481 gacgatggcg tcgagcggga tgtcctggag cgtgctcgtg ctggcctcga cgcccttgag
      541 ccgcttgcgc acctcgtcgt acctgaggag cctgtcgctc tgaccggaga gcagggccat
      601 gacgccggcc aggtcggccc tacgcctggc gttcctgaag tcggcgatgg ctccgttggc
      661 ggcagtgacg cgctcgtcca cgcgggcatt ctgccatgct gccctggtag ccccgtgagc
      721 ggcgcacgcc gccggctccg gttactctcc ggcatgaagg tcgagatctg gtccgacata
      781 gcctgcccct ggtgctacat cggcaagacg cggttcgaga aggcgctctc cgcctacgag
      841 cacgccggcg aggtcgaggt cgtctggcgc tccttcgagc tccagcccga cgcgtcccgc
      901 cagtcgccgg gcaccactgc aacccacctg atggacaagt acggccgctc gcacgacgag
      961 gtgctcggca tgatggaacg cgtgagcgag gtcgcggcct ccgaggggct cgagttccac
     1021 ctggagcgcg gcgtcgccgc caacaccttc gacgcccacc ggctcgtgca ctacggcgcg
     1081 agcgtcggca agggggaggc cgtcatgcgg cgcctcatgc gcgcgtacca gagcgacggc
     1141 gaggacctgt ccgatcacgg gacgctcgcc cggttggccg tcgaggccgg cctcgacgcg
     1201 agcggcgccc gcgacgtgct ggccggcgac gcgttctcgg acgccgtgcg ctccgacgag
     1261 cgccgcgccc gctcgctcgg cgtcgacggc gtgccgttct tcgtgttcga cgaggtgcac
     1321 ggcgtgtctg gcgcccagcc gacggagttc ttcctctcgg ccctcaggca gctcgggccg
     1381 caggtgaggc cactgaccat gcacggctcc gccgcgcccg tgtgcgacga ggacggctgc
     1441 gaggtgccgc agggttgagc ggcgctgggc gctgacggac cagcggctcc tgggccgctc
     1501 gcccatcagc gccgccggtc accacggccg ctccctaggg cctggcgcct gcgccggtgc
     1561 tatacttccg cgcagtatgg cgtcgcgaat gctggacacc cgccctgtca gcggctgacc
     1621 ctcagggtcg gtccgcccag ggcgccggtc ggcgccgaac aaacgaggcg ccgtgcaccg
     1681 caaccggcgc ctttcttgtt gccgcccgca gctcctctgg cgttcgtcac agccggccgg
     1741 ggtcgggggc ggcggccaac tcagccgcat aggcgagtgc ggcacggagg acacgcgaca
     1801 tgccggacac cgttgtcgac ctaggcgcgc agagcgagca gagcccctca caggccccgg
     1861 cgctgcgcga ccgcggcacg tactacgcca cgacgcccat cttctacgtg aacgcggcgc
     1921 cccacatcgg ccatgcgtac acgaccatcc tcgttgacgt cgtgacgcgc taccaccgcc
     1981 tgaaaggcga cgacaccttc ttcctgaccg gcaccgacga gcacggcgag aagatccaga
     2041 aggcggccga ggcggccggc gagtcgccgc aggcctacac ggatcaggtg agcggcgagt
     2101 tccgcgccgc gtgggaccgg ctcggcatcc gctacgacga cttcatcagg accaccgagc
     2161 cgcgccacaa gaaggtcgtg caggaggtcc tgcagcgcgt atacgacaac ggcgacatcg
     2221 tcttcggcga gtacgcgggc ctctactgcg tcaagtgcga gcgctactac accgacaagg
     2281 agctcgtgga cggcaagtgc ccccagcacg agatcgagcc cgagtaccgc acggaggcca
     2341 actacttctt ccgcatggag aagtaccgcc tatggctccg cgacctcctc accgaccagc
     2401 cggacctcat ccgacccgag cgctaccgca acgaggtcat ggcg
//
LOCUS       DAOQYE010000247         2438 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_48254_length_2438_cov_3.124633, whole genome shotgun sequence.
ACCESSION   DAOQYE010000247 DAOQYE010000000
VERSION     DAOQYE010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48254_length_2438_cov_3.124633"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..582)
                     /locus_tag="PKN52_04965"
     CDS             complement(<1..582)
                     /locus_tag="PKN52_04965"
                     /inference="COORDINATES: protein motif:HMM:NF020916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HNQ99328.1"
                     /translation="MTARADEIERLGAAADSFPRIDLRELQGASLMNRVDRKFVVHLP
                     ELVAVLEAARGSYRALSLEGEVWQPYHTRYFDTPGFGLLRAHHRGKTPRAKVRTRVYL
                     NSGLSFLEVKNRLNDGRTVKRRVETSDAARAPETEELSGLVLADLASEAAREGVTGNR
                     LETKLLNGYRRLTLVHGARAERVTIDTDLTFSTG"
     gene            811..1524
                     /locus_tag="PKN52_04970"
     CDS             811..1524
                     /locus_tag="PKN52_04970"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNQ99329.1"
                     /translation="MSQAQANTAVLAGRRVLIVEDEESLADIIELYLRQAGCSTEKAR
                     DGLRALDYHDRWRPDLLILDLGLPLLDGLEVLRRIREKDTTPVLILTARAEEADEIAG
                     LHLGGDDYLVKPVSARRLLSRVAALMRRAYLAEGRSAIHVGAVEIDSYSHRVTVGGSA
                     VDLTPTEYRLLEHLAATPDRVISRAELIEHAMPESDALERAVDIHLTHLRRKLREAGA
                     GEPIRTVRGVGYSLGADGG"
     gene            1521..>2438
                     /locus_tag="PKN52_04975"
     CDS             1521..>2438
                     /locus_tag="PKN52_04975"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HNQ99330.1"
                     /translation="MNRLVWRTALAMLVVVALAISIVPLAQRVADWRTLSSLPDDFKG
                     RVQKEMGPPHPPPAYGEAAGPDDPNFFQEESRRLFDFLAQQRRARNEAVVVGLLLAVG
                     VAGLLALWLSRSLVRPIEAVSGTVKKLATGDWSVRIVEPSGRWPEEARELATDFNSLA
                     EALERYEGGRQAMLADTAHELRNPLAAMQLRLEAISDGIVPASKEELAVLQNHVALLT
                     RLVQDLRTLSLAESHHLQLEPQDLDVGDLARDVIAMLRPMAAKHGVELVAEVEPVTVR
                     ADPSRLQQVVMNLLDNAIAVSPDGGVVTVR"
BASE COUNT          363 a          869 c          834 g          372 t
ORIGIN      
        1 acccgtggag aacgtgaggt cggtgtcgat ggtcacgcgt tcagcacgcg ccccgtggac
       61 gagggtgagg cgccgatagc cgttcaggag cttcgtctcc aggcggttgc ccgtgacacc
      121 ttcgcgagcg gcctccgagg ccaggtcggc gagcactagg ccagagagct cctccgtctc
      181 cggcgctcgg gcggcgtcgc tcgtctcgac ccggcgcttg accgtgcgac cgtcgttgag
      241 ccggttcttc acctccagga aggagaggcc gctgttcagg tacacgcgtg tgcgcacctt
      301 ggctcgcggc gtcttgccgc ggtgatgggc tctgaggagg ccgaagccgg gcgtgtcgaa
      361 gtagcgcgtg tgatagggct gccacacttc gccttccagg gagagcgcgc gatacgaccc
      421 gcgcgccgct tcgaggacgg ccaccagttc cggcaggtgg actacgaact tccggtcgac
      481 gcggttcatc aggctggctc cttgcagctc cctgaggtcg atcctgggga aggagtccgc
      541 cgccgcgccg agcctctcta tctcgtctgc tctcgctgtc atgcctcgga tgctaggcaa
      601 ggactgtttc ggttgcgttt gacgaccaga tcgccgtctt gcctgccacc gtcttgccta
      661 ccgtccgacc cggtgccgcc cggcccgccg ttgccgacct gcagttgtcc gaccctctgc
      721 cgcccgctcg gcggccgctt ccggccccga cctcctaccg gcatggggcg aaacggaacc
      781 tgaacacgcc ggcgctatgc ttcacgcacg atgtcacaag cccaagcaaa cacggcggtg
      841 ctcgcgggga gacgcgtgct gatagtcgag gacgaggaga gcctcgccga catcatcgag
      901 ctctacctgc gtcaggccgg ttgcagcacc gagaaggccc gcgacgggct gcgggccctc
      961 gactatcacg acaggtggcg gcctgatctg ctcatcctcg acctggggct gccgctgctg
     1021 gacgggctcg aggtgctgcg gcgcatcagg gagaaggaca cgacgcccgt cctcatcctc
     1081 acggcccggg cagaggaggc cgacgagatc gccggcctgc acctgggcgg cgacgactac
     1141 ctcgtgaagc ccgtcagcgc caggcggctc ttgagccgcg tggccgcgct catgcggcgg
     1201 gcgtacctgg ccgaggggcg gtcggccatc cacgtcggcg cggtcgagat cgactcgtac
     1261 tcgcaccgcg tcaccgtcgg cggctccgcc gtggacctga cgccgaccga gtaccgcctc
     1321 ctggagcacc tcgccgccac gccggacagg gtcatctcgc gcgccgagct catcgagcac
     1381 gccatgcccg agagcgacgc gctcgagcgc gccgtcgaca tccacctgac gcacctgcgt
     1441 aggaagctgc gcgaggccgg cgcaggcgag ccgatcagga ccgtccgcgg agttggctac
     1501 agcctagggg cggacggcgg atgaacaggc tcgtctggcg aacggcgctg gccatgctcg
     1561 tcgtcgtcgc cctggccatc tccatcgtgc cgctcgccca acgcgtggcc gactggcgca
     1621 cgctgtcgag cctgcccgac gacttcaagg gcagggtcca gaaggagatg ggcccgccgc
     1681 atccgccccc ggcgtacggc gaagccgccg gccccgatga tcccaacttc ttccaggagg
     1741 agagccgccg gctcttcgac ttcctcgcgc agcagcggcg ggcccgtaac gaggccgtag
     1801 tggtcgggct gctcctcgcc gtgggggtgg cggggctcct ggccctctgg ctgtcgcgga
     1861 gcctcgtgag gcccatcgag gccgtgtccg gcacggtcaa gaagctcgcc accggcgact
     1921 ggagcgtccg gatcgtcgag ccgtccggcc ggtggccgga agaggcgcga gagctggcta
     1981 ccgacttcaa cagcctcgcc gaggccctgg agcgctacga gggcggtcgg caggccatgc
     2041 tggccgacac cgcccacgag ctgcgcaatc cgctcgcggc catgcagctc cggctggaag
     2101 cgatcagcga cggcatcgtg cccgcctcca aggaggagct cgccgtgctg cagaaccacg
     2161 tggccctgct aacgcggctg gtccaggacc tgcggacgct ctcgctggcc gagagccacc
     2221 acctccaact cgaaccgcag gacctcgacg ttggcgacct cgccagggac gtcatcgcca
     2281 tgctgcgccc catggccgcc aagcatgggg tcgagctggt cgccgaggtc gagccggtca
     2341 ccgtgcgggc cgacccctcg cggctccagc aggtcgtcat gaacctgctc gacaacgcca
     2401 tcgccgtcag ccccgatggc ggcgtggtga ccgtgcgt
//
LOCUS       DAOQYE010000248         2436 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_48339_length_2436_cov_2.735405, whole genome shotgun sequence.
ACCESSION   DAOQYE010000248 DAOQYE010000000
VERSION     DAOQYE010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48339_length_2436_cov_2.735405"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            297..1232
                     /locus_tag="PKN52_04980"
     CDS             297..1232
                     /locus_tag="PKN52_04980"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNQ99331.1"
                     /translation="MSREPVSPSGNHLVAVLQALFVTLLWSSSWVLIKQGLGEIPPLT
                     FAGLRYALAAVVLWAALLASPVRRVAVRALDGRSWRNLVLLGLVMYSVTQGAQFVALA
                     YLPAQSVSLTLSLTPVLVALLGTFLLAEGLKTVQWVGVALVLVGVLVYFLPGRQAVGV
                     ASAGAAVGWLVAGVGALANAGSALLGRAVNRGGRLAPLLVTAVSMGVGALVLLGAGVA
                     VQGLPALSARGWLIVAWLALVNTAFAFTLWNLTLRRLSATESSVINNTMLVQIALLAY
                     FFLGEPLGAGQLVAFGLVVVGTVVVQVRRRPAGSA"
     gene            1324..1557
                     /locus_tag="PKN52_04985"
     CDS             1324..1557
                     /locus_tag="PKN52_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99332.1"
                     /translation="MMTTIKVPVALRERISANARSLGVTSATLIEAALDEYERRARWE
                     GVRRAYAALADDGGYLDEVREWDVVLADGLMDD"
     gene            1550..1903
                     /locus_tag="PKN52_04990"
     CDS             1550..1903
                     /locus_tag="PKN52_04990"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HNQ99333.1"
                     /translation="MTDQALPRGSVVWVDFDPVIGSEQSGLRPAVVVSSDPYNAFIPG
                     LVIVVPVTTRNRKLPHHVVLAGKSLGLTRQGFAMTEQLKTIDKRRIRGGAGRVDDAVL
                     NEIDRWLGDHLGLHA"
     gene            1991..>2436
                     /locus_tag="PKN52_04995"
     CDS             1991..>2436
                     /locus_tag="PKN52_04995"
                     /inference="COORDINATES: protein motif:HMM:NF025008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNQ99334.1"
                     /translation="MTASDTTSDAYFVRESATRLRPTKYVGGAWNPAEYHVAPLFGAL
                     AHAIEGDAASRGRGGLQVARVTFDILGVLPVEPVDIEVELVRPGRTIELVSAVLSHAG
                     RPAVVARAWLAAEFPTSHMAGTDLPGIPGPWELQPAQLGSYWYGEFV"
BASE COUNT          362 a          798 c          845 g          431 t
ORIGIN      
        1 gtggctagtc gaccgctcgc gggttacgct tgcctcgcga ccgaagggtg gctagggagc
       61 cgccggcacc gcacgagata gacacgacgc cgtgcatgac cgggccagga ccgagcgcca
      121 cataacgtcg acccgtgttt ggcacgcgcc gcgcaagcgg cggccgagac aggcaagcga
      181 gcgcgggcgg caggcacctc accggataaa agcctcgagg gggtaggtct cgcgcaggcg
      241 ctagctgggc catgattcgg accggcgagg ccgcgcgaac caactggagg cacggtatgt
      301 ctcgtgaacc cgtctcgccg tccggaaacc acctcgtcgc ggtcctgcag gcgctgttcg
      361 tgaccctgct ctggtcgagc tcatgggtgc tcatcaagca gggactcggc gagatcccgc
      421 cgctgacctt cgccggcttg cgctacgcgc tcgccgcagt cgtcctctgg gcggccctgc
      481 tcgccagtcc ggtgaggcgg gtggccgtcc gagcgctgga cgggcggtcg tggcggaacc
      541 tcgtgctgct cggcctcgtc atgtacagcg tcacacaggg cgcccagttc gtggccctcg
      601 cctacctccc cgcccagtcg gtgagcctca cgctctcgct cactccggtt ctcgtcgccc
      661 tgctcggcac cttcctgctc gccgagggcc tcaagaccgt tcagtgggtc ggcgtggccc
      721 tcgtgctagt cggggtcctc gtgtacttcc tgcccggtag acaagccgtc ggcgtcgcta
      781 gcgccggggc cgcggtcggt tggctggtcg ctggcgtagg cgcgctggcg aacgccggct
      841 cggcgctcct gggccgcgcc gtcaaccgcg gcggtcggct ggcgccgctc cttgtgacgg
      901 ccgtaagcat gggcgtcggc gcgctcgtgc tgctgggagc gggcgtggcc gtacagggcc
      961 tgccggcgct ctccgcgcgc ggctggctga tcgtggcgtg gctcgccctc gtcaacaccg
     1021 ctttcgcctt cacgctctgg aacctgactt tgcgcaggct ctcggccacc gagtcgagcg
     1081 tcatcaacaa cacgatgctc gtccagatcg ccttactcgc ctacttcttc cttggtgagc
     1141 cgctcggggc cgggcaactc gtggcgttcg gcctggtggt ggtcgggacg gtggtcgtgc
     1201 aagtgcggcg caggccggcg gggtctgctt gacctgtcgg ggatgggatc gagccgttgc
     1261 tctagatcgg agactcccca tgttcacgga tatcggaagt cataccgatg gtatgcttga
     1321 gcgatgatga cgaccatcaa ggtcccggtg gcgcttcgag agcgcataag cgctaacgcg
     1381 agatcgctcg gggtgacgtc tgccaccctg atcgaggctg cgcttgacga gtacgaacgg
     1441 cgcgcgcggt gggaaggcgt caggcgagcg tacgccgcgc tggcggacga cggcggttac
     1501 ctggacgagg tccgggagtg ggacgtggtg ctcgcggacg gcctgatgga tgactgacca
     1561 ggccttgccg cgcggcagcg tggtctgggt cgacttcgat ccggtgatag gaagcgaaca
     1621 gtccgggtta cggccggccg tggtggtgtc gtcagatcca tacaacgcgt tcatcccagg
     1681 cttggtgatc gtggttcccg tgacgacgag gaacaggaag ctgccgcacc acgtcgtctt
     1741 ggcaggtaaa agcctagggc ttacgcggca gggcttcgcc atgaccgagc aactcaagac
     1801 tatcgacaaa cgccgcatac gtggcggcgc cggtcgggtg gacgatgccg tcctcaacga
     1861 gatcgaccgt tggttgggcg accaccttgg cctccatgct taggactcga tagccactac
     1921 tcgctagcca ttacgtagcg atcgctttcg aaccggtcga cgaccctagg tgcgatgcga
     1981 tgatgtctcg atgaccgcaa gcgatacgac atcagacgcc tacttcgtgc gcgagtcggc
     2041 cacgcgcctg cgtcctacca agtacgtagg cggtgcctgg aaccccgccg agtaccacgt
     2101 cgcccctctc ttcggagccc tggctcacgc catcgagggc gatgccgcca gccgcggtcg
     2161 cggcggcctc caggtcgcac gcgttacctt cgacatcctc ggtgtgctcc cggtcgagcc
     2221 ggtcgacatc gaggtggagc tcgtgcggcc gggcaggacg atcgagctcg tcagcgccgt
     2281 gctgagccac gccggccggc cggcggtcgt cgcgcgggca tggttggcag cggagttccc
     2341 gacgagccac atggcaggca ccgacctgcc cggtatcccg ggtccttggg agctgcaacc
     2401 ggcgcaacta ggcagctact ggtacggcga gttcgt
//
LOCUS       DAOQYE010000249         2435 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_48356_length_2435_cov_3.092017, whole genome shotgun sequence.
ACCESSION   DAOQYE010000249 DAOQYE010000000
VERSION     DAOQYE010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2435
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48356_length_2435_cov_3.092017"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..726)
                     /locus_tag="PKN52_05000"
     CDS             complement(<1..726)
                     /locus_tag="PKN52_05000"
                     /inference="COORDINATES: protein motif:HMM:NF016145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HNQ99335.1"
                     /translation="MRVLLSGYYGAGNLGDEALLAGLAAGLIARGHEPVVLSVDPAAT
                     RALHGVAAVHRYSGLLPALLRADALVSGGGGLLQDATSGRSLAYYLGTLRLARALRKR
                     TVVYGQSLGPFTTSGRERTRRALTDVPLALRDKESVALASGMGFRSVLVADPALLMPL
                     PPGLSQDDGPGRRAGRPVVLAPRGGQEALNDALAGLARTLAAEGVPLAAIAFQPRQDG
                     PAVAKLAEAAPSIAVRAVATPAEA"
     gene            complement(775..>2435)
                     /locus_tag="PKN52_05005"
     CDS             complement(775..>2435)
                     /locus_tag="PKN52_05005"
                     /inference="COORDINATES: protein motif:HMM:NF039831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5693 family protein"
                     /protein_id="HNQ99336.1"
                     /translation="ELGRRYQQLGLTGIALYEDTIETLVAKGYAATLLGSELKAQLAA
                     DGQAAPAIPSDATLVTALMPGALDRLVAKNVPAARSFELDGRTWYLWPGDVKTFLPAG
                     PDTAQVTLWKNAGFDIAYRPRNAPYALTEVGNDFPSQASYLIHAGTQLTGWPSGLEEL
                     VSASQAYLTAVIEGTPQNGMANVSGKIPTVRLLSFNQDYVDRRLRPADLVDKYLLAVQ
                     ERNVRLLYLRPYTTVELGDPIANTEALVSSLSKTLRAQGYEVGPLTTLGKDYRTTTWL
                     RALAALGVLAGALLLGRRLPAPWGAAALAALLLLCAWAGGVSWDAVALLAALVFPTLG
                     FLTFRERWPAILGATLVSLAGALLLAAVGSERDTLLAIRPFSGVGLTLVVPPALFLVA
                     YMLRFHRPAQWVKRLVGMNITVAHVAVGLAAVAAVAIVLLRRGNDPVIGVSQFELTLR
                     SFLGEFVARPRFKELFGHPAALLALALLGWPAWLRGPLLTVGVVAQASILNSFSHYHT
                     PLLISLERTGVALLVGLVIGAVLVPVAALLTRLGNRWLASAEEP"
BASE COUNT          380 a          812 c          918 g          325 t
ORIGIN      
        1 cgcctcggcg ggggtcgcga cggcccgaac cgcgatgctc ggagcggcct cggcgagctt
       61 agcgacggcg gggccgtcct ggcgcggttg gaaggcgatg gcggcgagtg gcacgccttc
      121 tgccgccaag gtcctcgcta ggccggccaa cgcgtcgttc agggcctcct ggcctcccct
      181 gggcgcgagc acgacgggtc gacccgcccg ccggcccggc ccgtcgtctt gtgacaagcc
      241 gggcggcagt ggcatcagca gcgcggggtc ggcgacgagc acggaccgga aacccatgcc
      301 ggacgcgagc gccaccgact ccttatccct gagcgcgagc gggacgtcgg tgagcgcccg
      361 tctcgtgcgc tcgcgtccgc tcgtcgtgaa cggtccgagc gactgaccgt agacgacggt
      421 ccgcttccgc agggccctgg cgagccgcag tgtgccgagg tagtacgcga ggctgcgtcc
      481 gctcgtcgcg tcctggagga gaccgccgcc gcccgagacg agcgcgtctg ccctgagcag
      541 ggcggggagg agacccgagt aacgatggac cgccgcgacg ccgtgaaggg cgcgcgtcgc
      601 ggccgggtcg acggagagta cgaccggctc gtggccgcgg gcgatcaggc cggcggcgag
      661 gccggccagg agcgcctcgt cgcccaggtt gccggctccg tagtagccgc tcagtagtac
      721 gcgcatggtc agcgggggct caggacgcgc ccgacgtgaa tcgtcaccgc ttcctcacgg
      781 ttcctcggcg gacgccagcc agcggttgcc gagcctggtg agcagcgccg ccaccggcac
      841 gagcacggcg ccgatgacga gcccgacgag gagggcgacg ccggtgcgtt ccaatgagat
      901 gagtagtggc gtgtggtagt ggctgaacga gttcaggatg ctcgcctgcg ccactacgcc
      961 gacggtcaag agggggccgc gcagccacgc gggccagccg agaagcgcca gcgccaagag
     1021 cgcggccggg tgtccgaaga gctccttgaa gcgcgggcgc gccacgaact cgcccaggaa
     1081 cgatctcagc gtgagctcga actggctgac gcctatgacg gggtcgttgc cgcgccgcag
     1141 cagcacgatg gcgacggccg cgaccgctgc gagtccgacc gccacgtggg cgaccgtgat
     1201 gttcatgccg acgaggcgct tgacccactg cgcggggcgg tggaagcgca acatgtacgc
     1261 gacgaggaac aacgccggcg gcaccacgag cgtgagcccg acgccggaga acggtcggat
     1321 agcgaggaga gtgtcacgct cgctgccgac ggccgcaagg agcagggcgc cagcgaggct
     1381 gacaagggtc gcgccgagga tggcgggcca gcgctccctg aacgtcagga agccgagggt
     1441 ggggaagacc agcgcggcta ggagggcgac ggcgtcccag gagacgcccc cggcccacgc
     1501 gcagagcagt agtagggcgg cgagtgccgc ggcaccccac ggggcgggga gtctccgccc
     1561 gagcaacagg gcgccggcga ggacgccgag cgcggccagc gcgcgcagcc acgtggtcgt
     1621 gcggtagtcc ttgcccaacg tagtaagcgg cccgacctcg tagccctgcg cccgcagcgt
     1681 cttgctgagc gacgacacca gggcctcggt gttggcgatc gggtccccga gctcgaccgt
     1741 cgtgtaaggc cgtaggtaga ggagccgcac gttgcgctcc tggaccgcca gcaagtactt
     1801 gtcgacgaga tcggcgggcc tgaggcgtct gtcaacgtaa tcctggttga agctcaggag
     1861 ccgcaccgtg gggatcttgc ccgacacgtt cgccatgccg ttctgcgggg tgccctcgat
     1921 cacggcggtc aagtacgcct gtgacgcgct cacgagctcc tcgagcccgg acggccaccc
     1981 ggtcagctgg gtgcccgcgt ggatcagata actcgcctgg gaagggaagt cgttcccgac
     2041 ttccgtgagc gcgtagggcg cgttgcgtgg gcggtacgcg atgtcgaagc cggcgttctt
     2101 ccagagcgtc acctgcgcgg tgtccggccc ggccggcaag aacgtcttca cgtcccctgg
     2161 ccacaagtac cacgtgcggc cgtcgagctc gaaactgcgg gcggccggca cgttcttggc
     2221 cacgaggcgg tcgagcgccc ccggcatgag ggcggtgacc agcgtggcgt ctgacggtat
     2281 cgccggagcg gcctgcccgt cggctgcgag ctgggccttg agttcggagc cgaggagcgt
     2341 ggcggcgtag cctttcgcca ccagggtctc gatggtgtct tcgtagagcg cgatgccggt
     2401 caggcccagc tgctggtagc gcctgcccag ctcga
//
LOCUS       DAOQYE010000250         2432 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_48449_length_2432_cov_2.570046, whole genome shotgun sequence.
ACCESSION   DAOQYE010000250 DAOQYE010000000
VERSION     DAOQYE010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48449_length_2432_cov_2.570046"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1044
                     /locus_tag="PKN52_05010"
     CDS             <1..1044
                     /locus_tag="PKN52_05010"
                     /inference="COORDINATES: protein motif:HMM:NF022702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HNQ99337.1"
                     /translation="SAERLKVTLVWTDVAATVQNNRTPGGNASTAGQTGPLDPNAAAD
                     GRGLNAGPDGVGQGTGAPAQAARLPFSFTRERPLAVLVDNVGGYPQTGLREASMIVEM
                     PVEGGLTRLMALYDRNFPRRVGPVRSARDYFVELAQTNQAVLVHDGGSPGAMIAIANS
                     SLPTLNAYSNGPLFAREGERSAPYNLYGSGPDLRSAMLRLVPETSQVVSGQVFQPTDA
                     AVSVTEVSVRYSGAYTSGFRYDAVLNAYRWVRDGAPASHPDGQIMLFDAVLVGEITAR
                     VLPGDTEGRLYIPLEGGDATLYLRGRAEPGRWVLSTGKGVQFRTLGGEVVDLAPFRTW
                     AMLTPTYQSRQEQ"
     gene            complement(1092..1880)
                     /locus_tag="PKN52_05015"
     CDS             complement(1092..1880)
                     /locus_tag="PKN52_05015"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNQ99338.1"
                     /translation="MRIVVIGDIHVQPDKLWRMLREAGLADEQNRPTEALRGTDTKLV
                     LLGDLVHAKSRQRYADLINVRRYDEYNPRHLQRAEAIQERFLREVKGFQERVPNGNMV
                     ILLGNHDFNAATVEQGPLRTDDVSHLEWKPGHGEELDPELRDWILAWPRELALEGLHF
                     AHVGPLPEHNVYDQAFYLENRRRWLQEDKDFLDGTPYRLGVYGHTPVRGGVNVASQGR
                     ALLLDTNGHKDEYAWLEIDTSNAEYRLRLHGLFFDEIVPRLAAG"
     gene            complement(1904..>2432)
                     /locus_tag="PKN52_05020"
     CDS             complement(1904..>2432)
                     /locus_tag="PKN52_05020"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing M23
                     family metallopeptidase"
                     /protein_id="HNQ99339.1"
                     /translation="AALKSANSLKGDNLYVGQQLRILPGSDIAPTKAVAAAPAPQAQG
                     DDALMWPLIGAITSRFGYRQLRVSGSNFHTGVDIDGETGDPIRAAHGGTVTLAGWHGG
                     YGNLVIVTYGNTEYYYGHASAIKVEVGDEVAAGDVIALVGSTGNSTGSHLHFEIRVDG
                     EMIDPLPRLETYAGR"
BASE COUNT          364 a          827 c          829 g          412 t
ORIGIN      
        1 tccgccgagc gcctcaaggt cacgctcgtc tggaccgacg tcgccgccac cgtccagaac
       61 aaccgaaccc cgggtgggaa cgcctcaacc gccgggcaga cggggccgct cgacccgaac
      121 gcggccgccg acggtcgcgg gctcaacgcg ggcccggacg gcgtcggcca ggggacgggc
      181 gccccggctc aggcggcgcg cttgcctttc tccttcacgc gggagcggcc cctcgccgtg
      241 ttggtcgaca acgtcggcgg ctatccccag accggcttgc gcgaggcgag catgatcgta
      301 gagatgccgg tcgagggcgg cctgacccgc ctgatggccc tctacgaccg caacttcccc
      361 aggcgggtcg ggccggtgag gagcgcccgc gactacttcg tggaactggc gcagaccaac
      421 caggccgtcc tggtgcatga cggtggttcg cctggcgcga tgatcgccat cgccaactcc
      481 agtctgccta cgctgaacgc atacagcaac ggccccctct tcgcgcgcga aggcgagcgc
      541 tcggccccat acaacctcta cggctccggg ccggatctca ggtcggcgat gcttcgcctc
      601 gtaccggaga cttcgcaggt ggtgagcggc caggtgttcc agcctactga cgcggccgtc
      661 agcgtcaccg aagtcagcgt caggtacagc ggcgcttaca cgtccggctt caggtacgac
      721 gcggttctga acgcctaccg ttgggtccgc gatggtgcgc cggccagtca ccccgacggg
      781 cagatcatgc tcttcgacgc ggtactggta ggcgagatca cggcgcgcgt tctacccggc
      841 gacaccgagg gccgcctcta tatcccgctg gaaggcggcg acgccacgct ctacttgcgt
      901 ggtcgggccg agcccggtcg ctgggtactc tccacgggca aaggcgtgca gttccgtacg
      961 ctcggtggcg aggtcgtcga tctcgccccg ttccgcacgt gggcgatgct gacgccaacc
     1021 taccagagcc gccaggagca gtaggcctag acacgcacta ctcgcgcgcc aagtagcggt
     1081 tcggggaccc ctcaaccagc ggccagccgc ggcacgatct cgtcgaagaa gaggccgtgc
     1141 aggcgcagcc ggtactcggc gttgctcgta tcgatctcga gccacgcgta ctcgtccttg
     1201 tgaccgttgg tgtccagcag tagcgcccgt ccttgcgacg cgacgttgac gccgccgcgc
     1261 acgggcgtgt gcccgtaaac gccaagccgg tagggcgttc cgtcgaggaa gtccttgtcc
     1321 tcctgcaacc agcggcgccg gttctccagg tagaaggcct ggtcgtacac gttgtgctct
     1381 gggagcggcc cgacgtgggc gaaatgcagc ccctccaacg ccaactcgcg gggccaggcg
     1441 aggatccagt cgcgcaactc cgggtcgagc tcctcgccgt gaccgggctt ccactcgagg
     1501 tgggagacgt cgtcggtgcg caggggaccc tgctcaaccg tggcggcgtt gaagtcgtgg
     1561 ttgcctagga gtatcaccat attgccgttg ggcacgcgct cctggaaccc cttcacctcg
     1621 cgcaggaacc gttcctggat cgcctcggcc cgttggaggt ggcgcgggtt gtactcgtcg
     1681 taacgcctga cgttgatgag gtcggcatag cgctgcctgc tcttcgcatg cacgaggtca
     1741 ccgaggagca ccagcttcgt gtcggtaccg cgcaatgcct ccgtgggccg gttctgctcg
     1801 tctgcgaggc cggcctcgcg gagcatgcgc cacagcttgt cgggttggac gtggatgtcg
     1861 ccgatgacga cgatacgcac tcggtcagct cacttccggt cgatcaacgc ccggcgtacg
     1921 tctcgaggcg gggtagggga tcgatcatct ctccgtcgac gcggatctcg aagtggaggt
     1981 gcgaccccgt cgagttcccc gtgctgccga cgagcgctat gacgtccccg gcggcgacct
     2041 cgtcgccgac ctcgaccttg atggcggagg cgtggccgta gtagtactcg gtgttgccgt
     2101 aggtgactat cacgaggttg ccgtacccgc cgtgccagcc ggccagggtg accgtaccgc
     2161 cgtgcgcggc ccggatcggg tcgcccgtct cgccgtcgat gtcaaccccg gtgtggaagt
     2221 tgctcccgct cacccgcagt tgccggtagc cgaagcgcga ggtgatggcg ccgatgagcg
     2281 gccacatcag cgcgtcgtcg ccctgcgcct gcggggccgg cgcggcggcg acggccttgg
     2341 tcggcgcgat gtcggagccg ggcaggatac ggagttgctg gccaacgtag aggttgtcac
     2401 ccttgagcga gttggcgctc ttgagggccg cg
//
LOCUS       DAOQYE010000251         2428 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_48585_length_2428_cov_2.450906, whole genome shotgun sequence.
ACCESSION   DAOQYE010000251 DAOQYE010000000
VERSION     DAOQYE010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48585_length_2428_cov_2.450906"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(85..897)
                     /locus_tag="PKN52_05025"
     CDS             complement(85..897)
                     /locus_tag="PKN52_05025"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNQ99340.1"
                     /translation="MIYDSAPDIYDLQYATYREDVPFYLRLADEYGGPILELGCGTGR
                     VTEALARAGHAVVAVDASAAMLRRAAERLTGHDLVSLVTADMRELSLERTFPLVIAPF
                     NTLMHLYTLSDQDLALRAALDHLEPGGTFAFDVYQPHLGQLGVLRREGALAGGLATGA
                     PPFAGAPGAGTHQMAASTAGGRGAELFLLQEHDPERQLVESRYYLDELDEDGRLRRRT
                     ATLTQRYYGRFELERALRHAGFAEILMYGGFDRGRLLASSLGIVGIARKGGE"
     gene            complement(894..>2428)
                     /locus_tag="PKN52_05030"
     CDS             complement(894..>2428)
                     /locus_tag="PKN52_05030"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ99341.1"
                     /translation="LALWAQDALLGRAAARVGARWRERLYARLLAAAPGTLPATSGAL
                     SGRIVTDLREVETFYRFGLGSLIAESATLLLIVGLLLRADPRAALALVALALPAAGVL
                     RLLGRTLERVSRSALEGSEAVGHHLQEGLKHHELVRAFGAGDEMLRRFAAANRQVEVQ
                     SARRSLIAGLQVPATQVLVFTAIGVLVVILVNAVGRGALTVGDVVAFLTLVALAATPM
                     QLLPHSYAMYREARAAAARLRSLDDGASAAREPERPSTPPGAATFQLVDGAASRTGGA
                     QRGRSPAAPLLALRSVAYAYQPGVPVLERVNLELPPRGLVVVTGASGSGKTTLLRLLL
                     RFATPTTGTIELGVEDRPDPGHRPRPLQEVPEAELRRRLAYVPQGHELLSGRLRDALT
                     MGRAIGDAELWWALEGVGMAAAVRGSPGGLDALLGEDGGGFSGGQRQRLAIARALLGK
                     PDAVLLDEPTSNLDDDSEAEIVALLGRLATDRLVLAVTHRPALARAAELLVLAKPAGS
                     GA"
BASE COUNT          326 a          856 c          924 g          322 t
ORIGIN      
        1 tggagcggct gtcgctggaa gtgtgatggc ggtgcgggcc cggcctgacc ttcttaccgg
       61 acgccctgcc cgcgccggtg agccctactc tccgcccttg cgcgcgatcc cgacgatgcc
      121 gaggctgctt gccagcaggc gcccgcggtc gaagccgccg tacatgagga tctccgcgaa
      181 gccggcgtgg cgcagcgccc gttcgagctc gaaacgcccg tagtagcgct gcgtcaacgt
      241 cgccgtccgg cgcctcagtc tgccgtcctc gtccagttcg tccaggtagt agcggctctc
      301 gacaagttgg cgctcggggt cgtgctcctg cagcaagaac agctctgctc ctcggccgcc
      361 ggcggtggat gcagccatct ggtgcgtgcc cgcccccggg gcaccggcga agggaggggc
      421 gccggtggcc aacccgcctg ccagcgcccc ctcgcgcctg aggacgccga gctgccccag
      481 gtgcggctgg tagacgtcga aagcgaaggt gccgccgggt tccaggtgat cgagcgcggc
      541 ccgcagcgcc aggtcctggt cggagagcgt gtagaggtgc atcagcgtgt tgaacggggc
      601 gatgacgagc gggaaggtcc gctctaggga cagctcgcgc atgtcggcgg tgacgaggct
      661 cacgaggtcg tgccctgtga ggcgctcggc ggcgcggcgg agcatggccg ccgaggcgtc
      721 cacggccacg acggcgtggc cggcccgcgc cagcgcctcc gtcaccctgc ccgtgccgca
      781 gcccagctcg aggatgggcc cgccgtactc gtccgccagc cggaggtaga acggcacgtc
      841 ttcgcggtag gtcgcgtact ggaggtcgta gatgtcgggg gcggagtcgt agatcacgcg
      901 ccgctcccgg ccggcttggc gaggacgagg agctcggccg ctctggcgag cgccggtcga
      961 tgggtgaccg ccagcacgag cctgtcggtg gccaggcgcc ccagtagcgc cacgatctcc
     1021 gcctcgctgt cgtcgtcgag gttggacgtc ggctcgtcga gcagcaccgc gtccggcttg
     1081 ccgagcaggg ctctggcgat ggcaaggcgc tggcgctgcc cgccggagaa gccgccgccg
     1141 tcctcgccca gcaaggcgtc gagcccgcca ggggagcctc tgacggccgc ggccatgccg
     1201 accccttcca gcgcccacca cagctcggcg tcgccgatgg ccctgcccat ggtcagcgcg
     1261 tcgcgcaacc gaccggagag cagctcgtgg ccctgtggca cgtaggcgag gcggcgccgc
     1321 agctccgcct cgggcacttc ctgaagcggg cgaggacggt gtcccgggtc cggtctgtct
     1381 tccaccccga gctcgatcgt gcccgtggtc ggggtggcga aacgcaggag gaggcgcagc
     1441 agcgtggtct tgccgctgcc gctcgcgccc gtgacgacca cgagcccgcg gggcggcagc
     1501 tcgaggttca cgcgctcaag caccggtact ccgggctggt aggcgtaagc gactgagcgt
     1561 agggcgagga ggggcgcggc cggggagcgc ccccgttgcg caccgccggt tcggctcgcg
     1621 gccccgtcga cgagttggaa ggtcgcggcg cccggcgggg tggacggccg ttctggctcc
     1681 ctcgcggcgg aagcgccgtc gtcgagggag cgcagcctgg ccgccgccgc gcgcgcctcg
     1741 cggtacatgg cgtagctgtg gggcaggagc tgcatgggcg tggccgcgag ggcgacgagc
     1801 gtaaggaagg ccaccacgtc accgaccgtc agcgcgccgc gcccgacggc gttgacgagg
     1861 atgaccacca gcaccccgat cgccgtgaag accaggacct gcgtggcggg gacctgcagt
     1921 ccggcgatga ggctacgccg cgcgctctgc acttccacct ggcggttggc ggccgcgaag
     1981 cgcctgagca tctcgtctcc ggccccgaac gcccgcacga gctcgtggtg tttgaggccc
     2041 tcctggagat ggtggccgac ggcctcgctc ccctcgagcg cgctgcggct cacccgctcg
     2101 agcgtgcgtc cgaggagccg cagcacgccg gcggcgggca gggcgagggc cacgagagcg
     2161 agcgccgcgc gcgggtcggc cctcaggagg agaccgacga tgaggagcaa ggtcgccgac
     2221 tcggcgatga gcgagcccaa cccgaagcgg tagaacgtct cgacctcgcg caggtctgtg
     2281 acgatccggc cggagagggc cccgctcgtg gctgggagcg tgccgggcgc ggcggcgagg
     2341 agcctcgcgt agagccgctc ccgccagcgc gctccgacgc gcgctgccgc gcgcccgagg
     2401 agggcgtcct gggcccagag cgctaggc
//
LOCUS       DAOQYE010000252         2423 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_48722_length_2423_cov_2.968750, whole genome shotgun sequence.
ACCESSION   DAOQYE010000252 DAOQYE010000000
VERSION     DAOQYE010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48722_length_2423_cov_2.968750"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(430..1344)
                     /locus_tag="PKN52_05035"
     CDS             complement(430..1344)
                     /locus_tag="PKN52_05035"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ99342.1"
                     /translation="MEFLVVLAAAALRVATPILLAALGEMLAERAGVLNLGIEGTMLL
                     AAFVGFVAAHQSGSLWVGLAAAVATGALMGALMALLSVTLGLNQHVSGLGITLLGGGL
                     ALYFFRLLYGGASTPPSLGQSFGQLSVLPGTTLAPLLSQYGLTYFALLLVPVLALVLR
                     RTAFGLRLHASGENPEAVDIAGVNVYRVRYQALVLGGALMGLAGAFLTLAQLGAFTHG
                     VVNGRGWVAIAIVIFGNWRPVRILLGALLFGFVQALQLRLQAEGVALPYEILLALPYL
                     VTILVLALAGRNASYPAALLKPYRREGG"
     gene            complement(1344..>2423)
                     /locus_tag="PKN52_05040"
     CDS             complement(1344..>2423)
                     /locus_tag="PKN52_05040"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ99343.1"
                     /translation="APRLPWRWERGSAPWWLRLALPVIAILITFALASVLIAASGANP
                     LDVFYELLVRPLTRRTSRLEVLVRATPLLLTGVAVAIAFRAGYYNIGAEGQLYAGALA
                     AAFLGPRLGEWAPPAAVAAMLAGGLVAGAAWALLPAVLKVYAKVDEVVTTLLLNSVML
                     LLVGGLLNGPWRDPVSGWPRSPTIAAAAEFPQVIARSRVHLGFVVALVLVALFWWVLS
                     RTRFGLELRAVGMGATAARFMGVSVRSTVLVAALLSGGVAGLAGVSEVAGIHHYLIEG
                     ISPDYGYTGIIVATFGGLHALGVAVAAGFLALVDVGSTSASRALGIPAYLGQVVQATL
                     LLVTLAVLLLNRYRPARRSRREERA"
BASE COUNT          428 a          812 c          919 g          264 t
ORIGIN      
        1 cggcccttgt gtatccgtaa acaggggatc gtctccaatg ccttgaagta ggttcgttgt
       61 cgtctaggag catctgggtc cggaagcgcc gagacgtcgg ccgtgtagtg attgatcttc
      121 cgaatgtccg aaccggcggg catggctttc tgtacgaact gatagacgtt caaccacctc
      181 cgtccagggc gcgaacgggg ttctggcgcg ttcggccggc gtgcagatgc ggggacgtgc
      241 aggtgcaggg atgcgggggc acgagactat tgaaaggaaa tgcgcctatg cgcaacgcgc
      301 atcttgtgca acgtccgtgt tgcacaacat atcagttgca catgatgcgc gattcctaaa
      361 gacacctctg ctcccccgcg cccgtcgctc ccctagcgcc aagccccgcg cgcgccgcgc
      421 cggcgcccct cacccgccct cccgccggta aggcttgagg agcgcggcgg ggtagctggc
      481 gttgcgaccg gccagcgcca gcacgaggat cgtgacgagg tacggcagcg ccagcaggat
      541 ctcgtaaggc agcgcgacgc cctcggcctg gagccggagc tggagcgcct gcacgaagcc
      601 gaacaggagc gcgccgagca ggatgcgcac ggggcgccag ttgccgaaga tgacgatggc
      661 gatggccacc cagccgcgcc cgttcaccac gccgtgcgtg aacgcgccga gctgggcgag
      721 cgtgaggaaa gccccggcga gacccatgag cgcgccgccg aggaccagcg cctggtagcg
      781 cacgcggtag acgttcacgc cggcgatgtc gacggcctcg gggttctcgc ccgaagcgtg
      841 caggcgcaga ccgaacgccg tgcggcgcag cacgagagcc agcacgggca cgagcagcag
      901 cgcgaagtac gtcaggccgt actggctcag gagcggcgcg agcgtcgtgc caggcaggac
      961 gctcaactga ccgaacgact ggccgagcga gggcggggtg ctcgcgccgc cgtacaggag
     1021 gcggaagaag tagagcgcca gtccgccgcc cagcagcgtg atgccgaggc cggagacgtg
     1081 ctggttcagg ccgagcgtga cggacaggag cgccatgagc gcccccatga gcgcgcccgt
     1141 cgcgacggcc gccgcgaggc caacccagag ggagccgctc tggtgcgcgg ccacgaagcc
     1201 gacgaacgcg gcgagcagca tggtcccctc gatgccgagg ttgagcacgc cggcgcgctc
     1261 cgcgagcatc tcgccgagcg cggccagcaa gatgggggtg gccacgcgca gggccgcggc
     1321 cgcgaggacg acgaggaact ccatcaggca cgctcctctc ggcggctccg ccgcgcgggg
     1381 cggtagcggt tgagcagcag gacggcgagc gtgacgagga gcagcgtggc ctgcacgacc
     1441 tgcccgaggt aggcggggat gccgagcgcc cggctggccg aggtcgagcc gacgtcgacg
     1501 agcgcgagga acccggcggc cacggcgacc ccgagggcgt gcaggccccc gaaggtcgcg
     1561 acgatgatgc ccgtgtagcc gtaatcgggc gagatgccct cgatgaggta gtgatggatg
     1621 cccgccacct cgctcacgcc ggcgagcccc gctaccccgc cgctgagcag cgcggcgacg
     1681 aggacggtgc tgcgcacgct cacgcccatg aagcgggcgg ccgtcgcccc catgccgacc
     1741 gcgcgcagct cgagcccgaa acgggtgcgg gagagcaccc accagaagag cgccaccagg
     1801 acgagcgcca ccacgaaacc gaggtggacg cgcgagcggg cgatgacctg cgggaactcg
     1861 gccgcggcgg cgatggtggg cgaacgcggc cagcccgata ccgggtcgcg ccacggaccg
     1921 ttcaggagcc cgccgacgag caggagcatc acggagttga gcaggagcgt ggtaacgacc
     1981 tcgtcgacct tggcgtagac cttcaggacg gcgggcagga gcgcccaggc tgcgccggcc
     2041 accaggccgc cggcgagcat ggccgccacc gcggcgggcg gcgcccactc cccgaggcgg
     2101 gggccgagga aggcggcggc gagcgcgccg gcgtagagct gcccctcggc gccgatgttg
     2161 tagtagccgg cccggaacgc gatggcgacg gcgacgccgg tcagcaggag cggcgtcgcg
     2221 cgcacgagca cctcgaggcg cgaggtgcgg cgcgtgaggg gccggacgag cagctcgtag
     2281 aagacgtcga gcgggttggc gccggaggcc gcgatgagca cggacgcgag ggcgaacgtg
     2341 atgaggatgg cgatcacggg cagcgccagg cgcaaccacc acggcgcgga gccccgctcc
     2401 cagcgccacg gcaggcgcgg ggc
//
LOCUS       DAOQYE010000253         2420 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_48818_length_2420_cov_2.769133, whole genome shotgun sequence.
ACCESSION   DAOQYE010000253 DAOQYE010000000
VERSION     DAOQYE010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48818_length_2420_cov_2.769133"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2420
                     /locus_tag="PKN52_05045"
     CDS             <1..>2420
                     /locus_tag="PKN52_05045"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HNQ99344.1"
                     /translation="PGELLTRLGVDPAVGLTVETVETLRRRFGGNALDVGGSRPALAV
                     LVEQFRSVLVGLLVGAAGLSAYFGQVAEAVAVLVVLVLNALIGFVTELRAVRSVEALK
                     RLGTAHARVRRAGRPLTVAADDLVPGDILLVEGGDVVGADARVLKASRLEVDESSLTG
                     ESVPVAKGPAPVPADAPLPERASMLYKGTAVTRGTAEAMVVATGMATELGRIGRLVAG
                     AGGGGRGGGTPLEKRLDGMAHRLVWLTLAVAALVTLTGLGSGKHWLVLLETAIALAVA
                     TVPEGLPIIATLTLAQGVRRMARRNALVNRLSAVETLGATSVIITDKTGTLTENRLAV
                     SSLWLPAGEVVFGAADSAAAVATLGETHGAGPGRSEQEPGGREVDRQLRHALEAASLC
                     VTAELLPPASGATGSDQAGGGGVGDPLELALLHAARAAGIERAELLARYPELRQEAFD
                     SNTRLMATTHRLGVDGGDGAAGARAWLIVKGAPGAVIAASTSLADGRATPIDDRDREV
                     WLDRNRALAARGLRVLALAERAMGATGEGATSEGAAGAGAGAVNGAAAAPGAAAPAAT
                     EPMYHDLTLIGLVGLADPPRGEARRVVERCRAAGIRVVMATGDQATTAGAIAAAVGLA
                     DPQRVVDGRAMTRLLAGDPRGKDELLETSVFARMTPEQKLELIALHQEAGNVVAMTGD
                     GVNDAPALRQADIGVAMGRAGTDVAREAADMVLLDDSFGTIVKAVREGRIVFENIRAF
                     VLYLLSCNLSEVLVVGLAAVFGYPLPLLPLQILFLNLVTDVFPALALGAGRGDANGLT
                     RPPRPAAE"
BASE COUNT          325 a          852 c          890 g          353 t
ORIGIN      
        1 cacccggcga gctgctgacg cgcctcggcg tcgatccggc ggtcggtctc accgtcgaga
       61 cggtcgagac cctgaggcga cgcttcggcg gcaacgcctt ggacgtcggt gggagccgcc
      121 cggccctcgc cgtgctagtc gagcagttca ggtcggtcct cgtcggtctc ctggtcgggg
      181 cggccggcct gtcggcctac ttcggacagg tcgccgaggc agtggcggtg ctcgtggtgc
      241 tcgtcctgaa cgccctcata ggcttcgtga cggagctgcg ggccgtgcga tcggtagagg
      301 cgctcaagcg cctcggcacc gcgcacgcca gggtcaggcg cgccggccgg ccgctgaccg
      361 tcgcggcgga cgacctggtt cctggcgaca tcctcctcgt ggagggcggc gacgtcgttg
      421 gcgccgacgc gcgggtgctc aaggccagcc ggctcgaagt cgacgagtcc agcctcacgg
      481 gcgagtccgt gccggtcgcg aaagggcccg ccccggtccc ggccgacgcg ccgctcccgg
      541 aacgagcctc gatgctctac aaggggacgg ccgttacgag aggaaccgcc gaggccatgg
      601 tcgtggcgac cggcatggcc acggagctcg gtcgcatcgg tcgcctcgta gcgggtgccg
      661 gcggcggcgg tcggggcggc ggcacgcccc tcgagaagcg cctcgacggt atggcgcacc
      721 gcctcgtgtg gctgacgctg gcggtcgcgg cgctcgtgac cctcaccgga ctcggcagcg
      781 gcaagcactg gctcgtcctg ctggagactg ccatcgcgtt ggcggtggcc accgtgccgg
      841 aaggcctgcc gatcatcgcc accctgactc tcgctcaagg cgtgaggcgc atggcgcgtc
      901 gcaacgccct cgtgaaccgg ctgtctgccg tggagacgct gggcgccacc agcgtgatca
      961 tcaccgacaa gaccgggacg ttgacggaga accgcttggc cgtctcgagc ctatggctgc
     1021 cggcaggcga ggtcgtgttc ggtgccgccg actccgccgc cgctgtggcc acgctcggcg
     1081 agacccacgg cgctggccct ggccgaagcg agcaggagcc gggtggaagg gaagtagatc
     1141 gacagcttcg gcacgctctc gaggcggcgt ccctctgcgt gacggccgag ctgctgccgc
     1201 cggccagcgg ggcgaccggt tcggaccaag cgggcggcgg cggggtgggc gacccgttgg
     1261 agctggcgct gctgcacgcg gcgagggccg ccggcatcga gcgcgccgaa ctcctggcgc
     1321 gctaccccga gctgcgtcag gaagctttcg actccaacac gcgcctcatg gcaaccaccc
     1381 accgcctcgg cgtggacggg ggcgacggag ccgcgggcgc tcgggcttgg ctgatcgtca
     1441 agggagcccc gggcgccgtc atcgccgcct cgacgagcct ggccgacggc cgggctacgc
     1501 cgatcgacga ccgtgaccgt gaggtctggc tcgaccgcaa ccgcgcgttg gcggcacgtg
     1561 ggctgcgcgt gctggcgttg gccgagcgcg ccatgggcgc taccggtgaa ggagctacca
     1621 gtgaaggcgc tgccggcgcg ggcgctggcg ccgtgaatgg tgcggcggca gcgcctggtg
     1681 ccgccgcacc cgccgcgacg gaacccatgt atcacgatct cacgctgatc gggctagtcg
     1741 gcttggccga cccgccacgt ggcgaggcgc gtcgggtagt cgagcgttgc cgcgctgccg
     1801 gcatacgcgt cgtcatggcg acgggcgacc aggcgactac ggccggcgcc atcgctgccg
     1861 ccgtcggtct ggccgatccg caacgggttg tcgatggccg cgccatgaca cgactactcg
     1921 ccggcgatcc tcgagggaag gacgaactcc tcgagacctc cgtcttcgcc cgcatgacgc
     1981 ccgagcagaa gctcgaactg atcgctttgc atcaggaggc cgggaacgtc gtagccatga
     2041 cgggcgacgg cgtcaacgac gccccggccc tgcgtcaggc cgacatcggc gtggcgatgg
     2101 gacgcgccgg cacggacgtg gcgcgcgagg cggccgatat ggtcctgctg gacgactcct
     2161 tcggcacgat cgtgaaagcc gtgagggagg gccggatcgt gttcgagaac atccgcgcct
     2221 tcgtcctgta cctcctctcg tgcaacctca gcgaggtcct ggtggtcgga ctcgcggccg
     2281 tgttcggcta ccctctgccg ctcctgccgc tccagatcct gttcctcaac ctcgtgacgg
     2341 acgtcttccc ggcgttggcg ctcggcgccg gccggggcga cgcgaacggc ctcacgcgcc
     2401 ccccgcgtcc ggccgccgaa
//
LOCUS       DAOQYE010000254         2418 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_48884_length_2418_cov_2.758358, whole genome shotgun sequence.
ACCESSION   DAOQYE010000254 DAOQYE010000000
VERSION     DAOQYE010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48884_length_2418_cov_2.758358"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..686
                     /locus_tag="PKN52_05050"
     CDS             <1..686
                     /locus_tag="PKN52_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99345.1"
                     /translation="ITSASARRLPVEAVCAELGDRIPILIDGAHAPGLLDAPVPAGAA
                     FWLGNLHKWAFAARTAAALVVAPSWRERVRPLVASAGEARGFPGSFSYLGTQDPTAYL
                     ALPAALGFPVEYLDLTFRQLRERNATILELGVRRLAAALGLPTPVSNGLPMTVVPLGR
                     AGGDPEASVLGSRLREQGIEVAITSVGGQLHARVSVQAYVGLGEFDALATALADHLLP
                     THLGGAQPV"
     gene            complement(648..1055)
                     /locus_tag="PKN52_05055"
     CDS             complement(648..1055)
                     /locus_tag="PKN52_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99346.1"
                     /translation="MGTLLAVFLVVSLNLAPDGAQSMTIDNYVVEGVGIYPDPTTFLL
                     TKNPDATWQLHDAQGNPWFVVAVDGPMLAILNASGGKDEVDLGAALGLPAEAWWEADA
                     IEPPGFSPIVLSHLSNGVNVALDGLRAAEVRWE"
     gene            complement(1189..>2418)
                     /locus_tag="PKN52_05060"
     CDS             complement(1189..>2418)
                     /locus_tag="PKN52_05060"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNQ99347.1"
                     /translation="WGERHVGNGWERRQRGHPWPRHHPGWRARRRHWGLRRRLTVTFA
                     FVALAAVALTAWLTLGAVLNAQRDLFTQTPAGEPGLQGWSATDHAIGGGSTGTARNAF
                     RTVTRTAMLAGFLAFLLATTVAGLVTRRLTRPLLALERGARRLAAGERGLRLDLPPDR
                     DELRSLTGAFNALVGDLERQEEWRRGMVADIAHDLRTPLAVLRSELEAMQDRVRPLDD
                     AGLARLHREVMLLSRLVDDLRTLSTAESGHLELAPREVRVADYLRGLLDSLAGRAAEV
                     GAAIHLEPVSEDLSTTFDPDRIARVLGNLLDNALNYATPGAIDVSARQEGDELRITVR
                     DHGPGLPPGDEARVLERFYRGDPSRTRNDTGSGLGLSIAKAITEGHGGRLEAANHPDG
                     GAEFTVVLPMTTERKRA"
BASE COUNT          345 a          789 c          891 g          393 t
ORIGIN      
        1 agatcacctc ggcttccgcc cggaggttgc cggtcgaagc cgtgtgcgcc gagcttggcg
       61 accggatccc gatactgatc gacggagcgc atgcccctgg gctcctggac gctcctgtac
      121 cggcgggagc cgcgttctgg ctcggcaacc tgcacaagtg ggccttcgcg gctcggaccg
      181 ccgccgcgct cgtcgtggcc ccgtcctggc gcgagcgtgt gcgtccgctc gtcgcgagtg
      241 ccggcgaggc ccgcggcttc ccggggtcgt tcagttactt ggggacgcag gaccccacgg
      301 cctatttggc gcttccggcg gcgctcggct ttccggtcga ataccttgac ttgacgttcc
      361 gccaactccg cgagcgtaac gcgaccatcc tggaactggg agtgcggcgg ctggcggcgg
      421 cattagggct gccgacgcct gtgagcaacg gcctgccgat gaccgtagtg ccactcggca
      481 gggcaggagg cgacccggag gcgtcggtac ttggcagccg cctgcgcgag caaggcatcg
      541 aggttgccat aacctcggtc ggtgggcagt tgcacgcgcg cgtttcggtg caggcttacg
      601 ttgggctggg agagttcgac gctttggcga cggcgctggc agatcaccta ctcccaacgc
      661 acctcggcgg cgcgcagccc gtctagcgcg acgttcacgc cgttagacag gtggctgagc
      721 acgatggggg agaagcccgg cggctcgatc gcgtcggcct cccaccacgc ctcggctggc
      781 aagccgagcg ccgcgcccaa gtcaacctcg tccttgccgc cgctcgcgtt caggatggcg
      841 agcatcgggc cgtcgaccgc cacgacgaac cacgggttcc cctgcgcgtc gtggagttgc
      901 catgttgcgt cggggttctt cgtgaggaga aaggtcgtgg ggtccgggta gatcccgacg
      961 ccctcgacca cgtagttgtc gatggtcatc gactgggcgc catcgggtgc caggttcagc
     1021 gagacgacta gaaagacggc gagaagcgtt cccacggtgg gacctccaaa ggaaataggg
     1081 tttagcgctg gtgtgcgagc gcattgtaat cagcgtcgag ccggagtcga gtgaccgttg
     1141 gtgcgactag aggtgctgtg tcaccgctag gaccgtcgcc attcggtact aggctcgctt
     1201 ccgttcggtc gtcatcggca agacgacggt gaactccgcg ccgccgtccg gatggttcgc
     1261 agcttcgagg cgcccgccgt gaccttcggt gatggccttg gcgatggaca gcccgagccc
     1321 agaccccgtg tcgttgcgcg tgcgcgacgg gtcgccgcgg tagaagcgct cgagcacgcg
     1381 agcttcgtcg cccgggggta ggccggggcc gtggtcgcgg acggtgatgc gcagttcgtc
     1441 accctcctgc cgggcgctca cgtcgatcgc tccgggcgtg gcgtagttga gcgcgttgtc
     1501 gagcaggttg ccgagcacgc gagcgatgcg gtcagggtcg aaggtggtgc tcaggtcctc
     1561 ggacaccggt tccaggtgga tggcagcgcc gacttcagcg gcccggccgg cgaggctgtc
     1621 gagcagcccg cgcaggtagt cggctacccg aacctccctg ggcgcgagtt ccagatggcc
     1681 gctctccgcg gtcgagaggg ttcggaggtc gtcgacgagg cgtgacagca gcatgacctc
     1741 gcggtgaagg cgcgcgagcc cggcgtcgtc gagggggcgc acgcggtcct gcatggcctc
     1801 gagctcgctg cggagaacgg cgagcggggt gcgcaggtcg tgggctatgt ccgcgaccat
     1861 gccgcgccgc cactcctcct gccgctccag gtcgccgacc agtgcgttga aggctccggt
     1921 gaggctgcgg agctcgtcgc ggtcgggcgg gaggtcgagc ctcaacccgc gctccccggc
     1981 ggcgagtcgc cgtgcgcccc gttcgagcgc tagcagcggt cgggtgaggc ggcgcgtcac
     2041 gagccctgcc acagtcgtgg ccagtaggaa ggccaggaag ccggccagca tggcggtgcg
     2101 ggtcaccgtg cggaaagcgt tgcgggccgt accagtggaa ccgccaccga tggcgtggtc
     2161 ggtggcggac cagccttgga gcccgggctc acctgccggt gtctgcgtga acaggtcgcg
     2221 ttgcgcgttg agcacggccc ctagggtgag ccaggcggtg agcgcaacag cggcgagcgc
     2281 cacgaacgcg aacgtcacgg tgaggcgacg acgcaggccc cagtgacgcc ggcgcgcgcg
     2341 ccagccgggg tgatgcctcg gccacgggtg cccacgttgg cgtcgctccc aaccgttccc
     2401 gacgtgccgt tcacccca
//
LOCUS       DAOQYE010000255         2417 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_48914_length_2417_cov_2.575783, whole genome shotgun sequence.
ACCESSION   DAOQYE010000255 DAOQYE010000000
VERSION     DAOQYE010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48914_length_2417_cov_2.575783"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            112..1674
                     /gene="zwf"
                     /locus_tag="PKN52_05065"
     CDS             112..1674
                     /gene="zwf"
                     /locus_tag="PKN52_05065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HNQ99348.1"
                     /translation="MSMAREHTVAAGDGAPDTDAQTAHNADQASRSVVRNGVAPACAF
                     VIFGITGDLAARKLIPALYQLHVNGELNRKTVIVGFGRKPLSEPELEGSLREALAREV
                     PNLDESAWRSLAERISYVHGDYASVEDFARLAAAVEGLDVAGLIFYTATPPSVYGAIA
                     RSLAGAGLSTESNGRFSRLVVEKPFGTDAVSAKALNDELLEHFSERQLYRIDHYLAKE
                     TAQNLGVLRFANSMFEPFWNNRYIDHVQITMIEPMGVEGRGQFYEDAGILRDVFQNHL
                     LQLLALVAMEPPVRFDARSVRDEKVKLFSAIACPDPKEAVLGQYVACNGMPGYRQEPG
                     VDPRSRQATFAAMRLTVENWRWAGVPFYLRSGKRLEAKASEIVLQFKAPPHVPFTLPA
                     PVRPDRLILRIVPDEGISLRFNGKRPGQRVELGRISLDFSYRQSFDGAIPDAYETLLL
                     DVMEGDATLFMRADEVEAQWAIIDPLLEHAERQETVLHFYEAGGRGPRAAYALLEDVG
                     RSWKRPAGVKTA"
     gene            1696..>2417
                     /locus_tag="PKN52_05070"
     CDS             1696..>2417
                     /locus_tag="PKN52_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HNQ99349.1"
                     /translation="MQRVRIVGIPMDLGAGRRGVDMGPSALRLARLAPALRELGHEVV
                     DLGNVDTPLAEASEHLPSVGRLHHADAIAATCRAAFERLHTLPADEFVIALGGDHSVS
                     MGTVPGLNLGRATGLLWVDAHADINTPATSPSGNLHGMPVAHLIGLGDERLTSIWGGR
                     RVVEPEHIVYIGLRSVDPAERELIRESGALAFSMKEVDQRGVAFVVAAALERLAGLER
                     VHVSFDADVLDARLLPEGGRAAA"
BASE COUNT          373 a          888 c          812 g          344 t
ORIGIN      
        1 cataaccgcg gccctctacg cgcgcttcgc gagtcgctcg gaggactcgt tcgccaaccg
       61 caccgtcgcg acgctgcgca accgcttcgg cggccacaag acgaggcgcg catgagcatg
      121 gcgcgcgagc acaccgtggc cgcaggcgac ggcgcacctg acacggacgc gcagaccgct
      181 cataacgccg accaggcctc ccgttccgtg gtgaggaacg gtgtcgcccc ggcctgcgct
      241 ttcgtgatct tcggcatcac gggcgacctg gccgcccgca agctcatacc ggccctctac
      301 cagctccacg tcaacggcga gctgaaccgg aagaccgtca tcgtcggctt cgggcgcaag
      361 ccgctcagcg agcccgagct cgagggcagc ctgcgcgagg ccctcgcgcg cgaagtgccc
      421 aacctggacg agtcggcctg gcgctccctc gcggagcgga tctcgtacgt ccacggcgac
      481 tacgcgagcg tggaggactt cgcgcggctg gccgccgccg tcgagggcct cgacgtggcg
      541 gggctgatct tctacacggc cacgccgccg agcgtttacg gtgccatcgc ccgctcgcta
      601 gcgggagcag ggctctccac cgagtcgaac ggccgcttct cgcgcctggt cgtcgagaag
      661 ccgttcggca ccgacgccgt atccgccaaa gctctgaacg acgagctcct ggagcacttc
      721 tccgagcggc agctctaccg catcgaccac tacctggcga aggagaccgc ccagaacctg
      781 ggcgtgctgc gcttcgccaa cagcatgttc gagccgttct ggaacaaccg ctacatcgac
      841 cacgtccaga tcacgatgat cgagcccatg ggggtagaag ggcgtgggca gttctacgag
      901 gacgccggca tcctgcgcga cgtgttccag aaccacctgc tgcagctcct ggccctcgtc
      961 gcgatggagc cgcccgtgcg cttcgacgcc cgcagcgtgc gcgacgagaa ggtgaagctc
     1021 ttcagcgcca tcgcctgccc cgaccccaag gaagccgtcc tgggccagta cgtggcctgc
     1081 aacggcatgc ccggttaccg gcaggagccc ggggtcgatc cgcgctcgcg ccaggcgacg
     1141 ttcgccgcca tgcgcctcac ggtggagaac tggcgctggg ccggcgtccc cttctacctg
     1201 cgctcaggca agcgcctcga ggcgaaggcg agcgagatcg tcctgcagtt caaggcgccg
     1261 ccgcacgtgc cgttcacgct ccccgccccg gtgcggccgg accggctgat cctgcggatc
     1321 gtcccagacg agggcatcag cctccgcttc aacggcaagc ggcccggcca gcgcgtcgag
     1381 ctggggcgca tcagcctcga cttctcgtac cggcagagct tcgacggggc catccccgac
     1441 gcgtacgaga cgctgctcct cgacgtcatg gaaggcgacg ccaccctgtt catgcgggcg
     1501 gacgaggtcg aggcccaatg ggccatcatc gatccgctcc tcgagcacgc cgagcgccag
     1561 gagacggtcc tgcacttcta cgaggccggc ggcagggggc cgcgggccgc gtacgcgctg
     1621 ctggaggacg tcggcaggag ctggaagcgt ccggcggggg tcaagaccgc ctgaggcgcg
     1681 tgctagcttg aacgcatgca acgcgtccgc atcgtcggca taccgatgga cctcggtgcg
     1741 gggaggcgcg gcgtcgacat gggtccgagc gcgctacgcc tcgcccgcct cgcccccgcc
     1801 ctgagagagc tcggccacga ggtcgtcgac ctcggcaacg tcgacacccc cctcgcagaa
     1861 gcgagcgagc acctgccctc cgtggggcgc ctgcaccacg ccgacgccat cgccgcgacc
     1921 tgccgcgcgg cgttcgagcg cctgcacacc ttgcctgcgg acgagttcgt catcgcgctc
     1981 ggcggcgacc actcggtgag catgggcacg gttcctggcc tgaacctggg gcgtgccacg
     2041 ggcctcctct gggtggacgc ccacgccgac atcaacacgc cggccaccag cccgtcgggg
     2101 aacctgcacg gcatgcccgt cgcccacctg atcggtctgg gagacgagcg cctgacgtca
     2161 atctggggcg gccgccgcgt ggtggagccc gaacacatcg tctacatcgg cctgcgcagc
     2221 gtcgacccgg ccgagcgcga actcatccgc gagtccggag ccctggcgtt ctcgatgaag
     2281 gaggtcgacc agcgcggggt cgcgttcgtc gtggcggcgg cgctggagcg gctggccggc
     2341 ctcgagcgcg tgcacgtctc gttcgacgcg gacgtgctcg acgcccgtct gctgcccgaa
     2401 ggcggtcgtg ccgctgc
//
LOCUS       DAOQYE010000256         2404 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_49326_length_2404_cov_2.535547, whole genome shotgun sequence.
ACCESSION   DAOQYE010000256 DAOQYE010000000
VERSION     DAOQYE010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49326_length_2404_cov_2.535547"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1242
                     /gene="hflX"
                     /locus_tag="PKN52_05075"
     CDS             <1..1242
                     /gene="hflX"
                     /locus_tag="PKN52_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HNQ99350.1"
                     /translation="LEEIDFLERVRALEEELARSDAAREVTRSSAERAVLIGLATHKE
                     GAGETESRMSELAELVRSAGGEIAATSVQHRDRSDARTLVGEGKLQEAVSLAFHEDAD
                     LLVFDRELSPAQAREIEAFTKLKVLDRTQVILDIFAQNARGREAQVQVELAQLHYQLP
                     RLAGRGQAMSRLGGGIGTRGPGETKLEVDRRKIRDRIANLEATVDAVSKRRGETRKQR
                     RRGNVMTVALVGYTNAGKSTLFNVLAKSDVLARDALFATLRPTSREGWLPELGGSGGK
                     VVFTDTVGFIRDLPDELVNAFRATLEELHDADLLLHVVDGATPGAPDRVDAVRRVLSD
                     LRLEVPGLVVLNKLDAADRGVIEELGCRFEAVAVSAVTGEGLAELRATLAERLGGSSA
                     VPQAASEPPSLTPLTGAPHGS"
     gene            1252..>2404
                     /locus_tag="PKN52_05080"
     CDS             1252..>2404
                     /locus_tag="PKN52_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis decarboxylase"
                     /protein_id="HNQ99351.1"
                     /translation="MSLTDLRAYVARLEERGQLVRVRQPVSTYLEMAEIADRLVKTGG
                     PAVLFERPVDRDGRSLSVPVAMNLFGTRERMAMALGVATLDDVGERIRSLLDVKLGGG
                     LLGLMSNLPKLREVATLPPKRVRSAPVQQVVKRGAEVDLGKLPVLTTWPKDGGPFLTL
                     PLVVTRDPERGDVNIGMYRMQVLDSKSTAMHWQRHKTGARHLESARRLGKPLEVAVAI
                     GGDPALIYAATAPLPPVPGLNEYSLTGFLRGRSVEVVKGVSVDLEVPAHAEIVLEGYV
                     DPFEEPVMEGPFGDHTGFYTLPDLYPRFHVTALTTRADPIYPATVVGPPPMEDAYLIE
                     ASERIFLEPARFILPEIADYHMPPAGIAHNLVNVRIRKSYPGQAYKVANG"
BASE COUNT          371 a          860 c          848 g          325 t
ORIGIN      
        1 ctggaagaga tcgacttcct ggagcgcgtg cgggccctcg aggaggagct ggcccgctcc
       61 gacgcggcgc gcgaggtgac caggagcagc gccgagcggg ccgtgctcat cggcctcgcc
      121 acccacaagg aaggggcagg cgagaccgag tcgcgcatga gtgagctcgc cgagctggtg
      181 aggagcgccg gcggcgagat cgccgccacg agcgtccagc accgcgaccg ttcggacgcg
      241 cgcacgctgg tcggcgaggg caagctgcag gaggccgtct cgctcgcgtt ccacgaggac
      301 gccgacctgc tcgtcttcga ccgtgagctc tcccccgccc aggcgcggga gatcgaggcc
      361 ttcacgaagc tcaaggtgct cgaccgcacg caggtgatcc tcgatatctt cgcccagaac
      421 gccaggggcc gcgaggcgca ggtgcaggtc gagctcgctc agctgcacta ccagttgcca
      481 cgcctcgccg gccggggcca ggccatgagc cgcttgggcg gcggcatcgg cacgcgcggt
      541 cccggcgaga cgaagctcga ggtggaccgc cgcaagatcc gcgaccgcat cgcgaacctc
      601 gaggccacgg tggacgccgt gagcaagcgc cgtggcgaga cgcgcaagca gcggcggcgc
      661 ggcaacgtca tgaccgtggc gctggtcggc tatacgaacg cgggtaagtc gacgctcttc
      721 aacgtcctcg cgaagtccga cgtcctggcg cgcgacgcgc tcttcgccac cctgcgtccg
      781 accagccggg aggggtggct ccccgagctg ggcggcagcg gcggcaaggt cgtgttcacc
      841 gacacggtgg ggttcatccg agatctcccc gatgagctcg tcaacgcctt cagggccacg
      901 ctcgaggagc tccacgacgc cgacctcctc ctccacgtcg tcgacggcgc gacccctggc
      961 gcgcccgacc gggtggacgc cgtgcgacgc gtgctatccg acctgaggct cgaggtgcct
     1021 gggctcgtcg tgctcaacaa gctcgacgcg gccgaccgcg gcgtcatcga ggagctgggg
     1081 tgtcggttcg aggcggtggc cgtgtcagcc gtgaccggcg aggggctcgc cgaactccgg
     1141 gccaccctgg cggagcgcct gggcggttcg agcgcggtgc cgcaggccgc ctcggagccc
     1201 ccgagcctca cgccactcac cggcgccccg cacggcagct agcgtgaccg catgagcctc
     1261 accgacctgc gggcgtacgt agcccggctg gaggagcgcg gccagttggt acgcgtccgg
     1321 cagcccgtgt cgacgtacct ggagatggcc gagatcgccg atcgcctggt caagaccggc
     1381 gggccggcgg tgctgttcga gcggcccgtc gaccgcgacg gacggagcct gagcgttccc
     1441 gtggcgatga acctgttcgg gaccagggag cgcatggcca tggcgctcgg cgtggccacg
     1501 ctcgacgacg tcggcgagcg catccgctcc ctactggacg tcaagctggg cggcggcctc
     1561 ctcggcctga tgtcgaacct ccccaagctc cgcgaagtgg ctaccctgcc cccgaagcgg
     1621 gtgcgctccg ccccggtgca gcaggtggtc aagcgcggcg cggaagtcga cctcggcaag
     1681 ctgccggtgc tgacgacgtg gccgaaggac ggcgggccgt tcctcacgct gcccttggtc
     1741 gtcacgcgcg acccggagcg gggcgacgtg aacatcggca tgtaccgcat gcaggtcctg
     1801 gactccaaga gcacggccat gcattggcag aggcacaaga cgggcgcacg ccatctcgag
     1861 tcggcgcggc gtctcggcaa gccgctcgag gtggccgtcg ccatcggcgg cgacccggcc
     1921 ctcatctacg cagccacggc gccgctaccg cccgtgccgg ggctcaacga gtactcgctg
     1981 acgggcttcc tgcggggcag gtcggtggag gtcgtcaagg gcgttagcgt cgacctcgag
     2041 gtccccgcgc acgccgagat cgtgctggag gggtacgtcg acccgttcga ggagcccgtc
     2101 atggaggggc cgttcggcga ccacacgggt ttctacacgc tgcccgacct ctacccgcgc
     2161 ttccacgtga cggcgctcac gactcgggcg gaccccatct accccgccac ggtcgtcggg
     2221 cccccgccca tggaagacgc ctacctaatc gaggcgagcg agcgcatctt cctcgagccg
     2281 gcgcgcttca tcctccccga gatcgccgat taccacatgc cgccggccgg catcgcgcac
     2341 aacctcgtga acgtgcgcat caggaagagc tacccaggcc aggcctacaa ggtcgccaac
     2401 ggcc
//
LOCUS       DAOQYE010000257         2397 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_49561_length_2397_cov_2.480786, whole genome shotgun sequence.
ACCESSION   DAOQYE010000257 DAOQYE010000000
VERSION     DAOQYE010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49561_length_2397_cov_2.480786"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            165..557
                     /gene="aroH"
                     /locus_tag="PKN52_05085"
     CDS             165..557
                     /gene="aroH"
                     /locus_tag="PKN52_05085"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HNQ99352.1"
                     /translation="MDGRERENRVRGIRGATTVEADVPEQILSATRELLEEMLAQNGI
                     SEYSEISSVFFTTTPDLSSTFPAEGARALGMTMVPLLCFQEIPVPNRLPRCVRVMLLL
                     NTTKAQDEMRHVYLREARSLRPDLVSAQ"
     gene            complement(560..1537)
                     /locus_tag="PKN52_05090"
     CDS             complement(560..1537)
                     /locus_tag="PKN52_05090"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNQ99353.1"
                     /translation="MLELTYAYYRLADLDGRQTPLPWLRELRDHPPKWLSASMARRDQ
                     ELGFEPLLIACAGGYEFDETPARFLTDFAELPSAVVADFETTFVRPDGADVVASENSA
                     AIKAALARLAEPGAARRLGATLESLWEHLRPTWEGAGRGATEEAARALKGAMGSDGDL
                     LASLPTHHFLQLEDSVNAIRRQSRRGKTLVVPLYFALRGGFRFSVRDQLVIGYGARTE
                     YFYEEQRDAVLTLANRLKAFSDPTRLLLLFHVGRLARFPLTVGDLAKQLGVSQPTASG
                     HLRLLRELGLVTVVRRGNRSYYRLERDAVRPLLEELERVLLPPASGHQR"
     gene            complement(1621..1902)
                     /locus_tag="PKN52_05095"
     CDS             complement(1621..1902)
                     /locus_tag="PKN52_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99354.1"
                     /translation="MFGMFRRQDPRFREEANALLCQVRTDRHGEIIRVRLSKTSEMSL
                     QGSNYFVRKSLVGPKTLDNATLEVTMTRSHKVTNATVDGGRLLAVRDWE"
     gene            1929..>2397
                     /locus_tag="PKN52_05100"
     CDS             1929..>2397
                     /locus_tag="PKN52_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HNQ99355.1"
                     /translation="MGTSTLRAALAAALAASEPGTCLGPYLPSPPAGRVVVVGAGKAA
                     AAMARAVEAGFEADVPMTGAVVTRYGHALPTRRVAVLEGSHPVPDAAGVAATARIRRL
                     VADADETDLIICLISGGGSALLCAPAGLGLAEKAETTAALLRGGADIRRINTVR"
BASE COUNT          353 a          839 c          837 g          368 t
ORIGIN      
        1 cggcgcgacc tccgatcgta ggaactcgcg taacggacct atcagggagc gctggtctgg
       61 cgtgagatcg aaccacggcg tgacgttctt gagcatgtcg tcaccgtaag gcaagcgccg
      121 ggagcagcgc agtaacgggt cgcgcgcctg gtacactccc cgccatggac ggacgggaac
      181 gagagaaccg cgtgcgaggc atacggggcg cgaccacggt cgaggccgat gtgcccgagc
      241 agatcctctc cgccacgcgc gagctgctcg aggagatgtt ggctcagaac ggcatctctg
      301 agtactcgga gatctcctcg gtgttcttca ccaccacccc tgacctgtcg tcgacgttcc
      361 cggccgaagg cgcgcgcgcc ctgggcatga cgatggtgcc cctcctatgc ttccaggaga
      421 tcccggtgcc gaacaggctc cccaggtgcg tgcgcgtcat gctcctactc aacacgacga
      481 aggcgcagga cgagatgcgc cacgtctacc tcagggaggc ccgcagcctg cggccggacc
      541 tcgtgagcgc gcagtgacgc tagcgttggt gtccagaggc cggcggtagg agcacccgct
      601 ccaactcctc cagcagcggg cgcacggcgt cgcgctccag gcggtagtac gagcggttgc
      661 cgcgcctgac gaccgtcacc aggccgagtt cgcgcagcag cctgaggtga ccgctggccg
      721 tcggctggct gacgcccaac tgcttggcga ggtcgccgac ggtgagcggg aaccgcgcga
      781 gcctcccgac gtggaagagg agcaggaggc gcgtcggatc ggagaaggcc ttcaggcggt
      841 tggcgagcgt gagtaccgcg tcgcgctgct cctcgtagaa gtactcggtc cgcgccccgt
      901 agccgatgac gagttggtca cggacgctga accggaaccc gccgcgtaga gcgaagtaca
      961 agggcaccac cagggtcttg ccgcgccttg actgccgcct gatggcgttg acgctgtcct
     1021 cgagctgaag gaagtgatgg gtgggtagcg aggcgaggag gtcgccgtcg gaacccatgg
     1081 cgcctttcag cgcgcgtgcc gcctcctcgg tcgcgccgcg accagcccct tcccaggtgg
     1141 ggcgcaggtg ctcccacagg ctctccagcg tcgcgccgag ccgccgggcc gctccgggct
     1201 ccgccaggcg cgcgagggcg gccttgatgg cggccgagtt ctccgaggct acgacgtcgg
     1261 cgccgtccgg ccggacgaag gtcgtctcga agtccgccac caccgcgctc gggagttcgg
     1321 cgaagtcggt caggaaccgc gccggcgtct catcgaactc gtacccgccg gcgcaggcta
     1381 tcaggagcgg ctcgaagccg agttcctggt cgcgcctcgc catgctcgcg ctcagccact
     1441 tgggcgggtg gtcgcgcagt tcacgcaacc acggcagcgg ggtctgacgc ccgtcgaggt
     1501 cggctaggcg gtagtaggcg taggtgagct cgagcacggg ggccggccgc acttccagcg
     1561 ccgcgctcgg tagagccggc ttctccgccg aagtggcagc cctccgcccc ccggccacgg
     1621 tcattcccag tctctgaccg ctaggagccg accgccgtcg accgtcgcgt tggtgacctt
     1681 gtgagacctc gtcatcgtca cttcgagagt ggcgttgtcg agcgtcttgg gcccgacgag
     1741 cgacttgcgg acgaaatagt tgctgccctg caggctcatc tcgctcgtct tcgagaggcg
     1801 cacgcggatg atctcgccgt gccggtcggt gcgcacctga catagcagtg cgttggcctc
     1861 ctcgcggaag cgcgggtctt gccgcctgaa catgccgaac atgccctatt ctagccaggg
     1921 tggccaccgt gggaacctcg acgttgcgcg cggcattggc cgcggcgctg gccgcgtccg
     1981 agcccggcac gtgcctgggc ccctatctgc cttcgcctcc cgcgggccgc gtcgtcgtcg
     2041 taggcgccgg caaggccgcg gcggccatgg cccgggccgt cgaggctggc ttcgaggccg
     2101 acgtgcccat gaccggcgcc gtcgtcacgc gctacggcca cgcccttccc acgcggcgcg
     2161 tcgccgtgct ggagggctcc cacccagtgc cggacgcggc cggtgtcgcc gccaccgcgc
     2221 gcatacgcag gctggtggcc gacgccgacg agacggacct catcatctgc ctgatctccg
     2281 gagggggctc cgcgctcctc tgcgcgcccg cgggtctcgg cctcgccgag aaggccgaga
     2341 cgacggccgc gctcctgcgc ggcggggccg acatccgtcg catcaacacg gtgcgca
//
LOCUS       DAOQYE010000258         2395 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_49626_length_2395_cov_2.802564, whole genome shotgun sequence.
ACCESSION   DAOQYE010000258 DAOQYE010000000
VERSION     DAOQYE010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49626_length_2395_cov_2.802564"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="PKN52_05105"
     CDS             complement(<1..276)
                     /locus_tag="PKN52_05105"
                     /inference="COORDINATES: protein motif:HMM:NF024248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNQ99356.1"
                     /translation="MLSTGAAAQPDDSDDDLVFRALASATRRRMLDALRRSPMTTGAL
                     CDLFPDLDRTTVLQHLRVLERAALVNGSKAGRERVLTLAPLPIKRIYD"
     gene            293..769
                     /locus_tag="PKN52_05110"
     CDS             293..769
                     /locus_tag="PKN52_05110"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNQ99357.1"
                     /translation="MSDSERLESLSFTVSARVSRPREEVYEAVADPAQLSRYFTTGGA
                     RGRLEPGAEVSWDFHDFPGAFPVMVLEAEPPRRIVIEWGGTNTADLSGRTRTTFEFEP
                     LDGNTRTLVTVTESSWLPSEAGAKSAFGNCEGWTGMLAALKAWLEHGINLREGYYK"
     gene            complement(801..1655)
                     /locus_tag="PKN52_05115"
     CDS             complement(801..1655)
                     /locus_tag="PKN52_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ99358.1"
                     /translation="MKGVGVGSSGPQGAGSKSAEAPDGRLRFGDVSLGYRDRLIVEGL
                     SLSLPWGQVTAIIGPNGSGKSTLLRALARLMRPRAGAAYLDGHAIHTLPTREVAKRLA
                     VLPQQPQAPEGLTVRELVEYGRFPLRRTLGGLTRDDRQAVAEALELTGTTDLSGEPVS
                     SLSGGQRQRAWIAMALAQGTELLLLDEPTTFLDMAHQLEVLELLKRLNADQGRTVVMV
                     LHDLGLASRYSHHLVALREGRVVTTGAPAEVVTPALLREVFEVEADVVVDPRSGAPIC
                     LPYAVLSR"
     gene            complement(1652..>2395)
                     /locus_tag="PKN52_05120"
     CDS             complement(1652..>2395)
                     /locus_tag="PKN52_05120"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HNQ99359.1"
                     /translation="LADPGIVGIHAGAGVGVMLLLVLPVSVREASALAQPVAAFVGAG
                     AAALLVYGFAFKDGGVTATRLLLVGVGVAAGLSAVMLLLALRMNDQVFAYAVAWQSGS
                     VAGKGWAPIRVLAPLLAALVPLALLQARRLDVLGFGRDVATGLGVAVERQRLGLLALS
                     VGLSASAVALAGSISFVGLIAPHMARSIVGPRHANLLPSAALLGGLLVLVADTAGRTL
                     FGTTELPAGVMVAAVGAPYFLYLLGRSKA"
BASE COUNT          397 a          857 c          836 g          305 t
ORIGIN      
        1 gtcgtagatc cgcttgatgg gcagcggcgc cagggtcagc acccgctcgc gccccgcctt
       61 gctaccgttc acgagagctg cccgctccaa cacgcgcagg tgctgcagga ccgtcgtcct
      121 gtcgaggtcc gggaacaggt cgcagagcgc tcccgtcgtc atgggcgagc ggcgcagggc
      181 gtccaacatc cgtcgccgcg tggcggatgc caaggccctg aacacgaggt cgtcgtcgga
      241 gtcgtcgggc tgcgcggccg cgccggtcga taacatgtga tataaatgta acatgtcaga
      301 ttcagagcgg ttggaaagcc tgtccttcac ggtctcggcg cgcgtctccc gtcctcgcga
      361 ggaggtctac gaggcggtcg ccgacccggc ccagctctct cgctacttca cgaccggcgg
      421 agcgcgcggg cgcctcgagc cgggcgccga ggtctcatgg gatttccacg acttccccgg
      481 cgcgttccca gtcatggtcc tggaggcgga gccgccccgc cggatagtca tcgagtgggg
      541 agggaccaac acggccgatc tctccggccg caccaggacg acgttcgagt tcgagccgct
      601 cgacggcaac acgcgcaccc tcgtgacggt caccgagtcc tcctggctgc cgtccgaggc
      661 gggcgccaag agcgccttcg gcaactgcga agggtggaca ggcatgctgg ccgcgctcaa
      721 ggcgtggctg gaacacggca tcaacttgcg cgaggggtac tacaaatagc ctgcggccgg
      781 aacgctcggc cgtccgacag ttagcgcgaa agcacggcgt aaggcaagca gatgggcgcg
      841 ccactccgcg ggtcgaccac gacgtccgcc tccacctcga acacttccct gagcagcgcg
      901 ggcgtcacca cctcggcggg ggcgcccgtg gtcacgacac ggccctcgcg cagcgccacc
      961 aggtgatggg agtagcggct ggccagcccg aggtcgtgga gcaccatgac gacagtcctg
     1021 ccctggtcgg cgttgaggcg cttgagcagc tcgagcacct ccagctggtg cgccatgtcg
     1081 aggaaggtcg tcggctcatc gagcaggagc agctccgtcc cttgcgccag ggccatggcg
     1141 atccacgcac gctgccgttg accgccggac agggaactca ccggctcgcc gctcaagtcc
     1201 gtcgtgccgg tgagttccag cgcctcggcg acggcctgcc tgtcatcgcg ggtcaggccg
     1261 ccgagcgtac gccgcagggg gaagcggccg tactcgacga gctcgcgcac cgtcagcccc
     1321 tcgggcgcct ggggctgctg cggcaagacg gcaaggcgct tggcgacctc gcgcgtcggc
     1381 aaggtatgga tggcgtgacc gtccaggtag gcggcgccgg ctcgcgggcg catgaggcgc
     1441 gccaaggccc gcagcagagt cgacttgcca gagccgttcg ggccgatgat ggccgtgacc
     1501 tggccccaag gcagggacag cgacagcccc tccacgatca gcctgtcccg atagccgagc
     1561 gacacgtcgc cgaaccgtag gcgcccgtcc ggcgcctccg cgctcttcga cccggcaccc
     1621 tgtggtccgc tactccctac tccgacgccc ttcaagcctt gctccttccg agcagataga
     1681 ggaagtacgg cgcgccgacg gcggccacca tcacgcccgc cggcagctcc gtcgtgccga
     1741 agagcgtgcg gcccgccgtg tcggccacca gcacgagcag cccgccgagg agcgcagcgg
     1801 acggcagcag gttggcatgc cgcggaccga cgatgctcct ggccatatgc ggggctatga
     1861 gaccgacgaa cgagatgcta ccggccagcg cgacggcgga ggccgacagc ccgacgctca
     1921 aggcgagcag gccgagccgc tggcgctcga ccgccacgcc gaggccggtc gcgacgtcgc
     1981 gcccgaaccc taggacgtcc aggcggcgcg cctgcaggag ggcgagcggt acgagggcgg
     2041 ccagtagcgg ggcgaggacg cggatgggcg cccaaccctt gccggccacg gagccgctct
     2101 gccaggcgac ggcgtaagcg aacacctggt cgttcatgcg caacgccagg aggagcatca
     2161 cggccgacag gccggcggcc accccgacgc cgacgagcag cagcctggtg gccgtgacgc
     2221 cgccgtcctt gaacgcgaaa ccgtagacga gtagcgccgc cgcgccggcg ccgacgaagg
     2281 ccgcgaccgg ctgcgccagc gcggacgcct cccgcaccga caccggcaac acgagcagca
     2341 gcatgacccc gaccccggca ccggcgtgga tgccgacgat gccggggtcg gcgag
//
LOCUS       DAOQYE010000259         2382 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_50003_length_2382_cov_3.296519, whole genome shotgun sequence.
ACCESSION   DAOQYE010000259 DAOQYE010000000
VERSION     DAOQYE010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50003_length_2382_cov_3.296519"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="PKN52_05125"
     CDS             complement(<1..348)
                     /locus_tag="PKN52_05125"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNQ99360.1"
                     /translation="MSEQGASSRLGAQPAEAQPARVLDVVDADLGYGELQVVFGVSLH
                     VNAGELVGLVGGNGSGKSTILRAVSGMIKPRGGKVFLAGEDVTGLKPHDLALRGLAHV
                     PMGRQLFGDMTVEE"
     gene            complement(341..1063)
                     /locus_tag="PKN52_05130"
     CDS             complement(341..1063)
                     /locus_tag="PKN52_05130"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ99361.1"
                     /translation="MPRALEAEHITVQFGGLVAVNDVSLALDAGQIVGLIGPNGAGKS
                     TLFNALTGYAKTKRGTVKLFGANVERLPPYARTRRGMGRTFQIERPFEGLTVLENVLI
                     PAFLHHATRSEAEAAARHALELVGLSDRAVQPSSDLNLARRRRLELAKALAVQPRVLF
                     LDELMAGLNPPALKEMIGFVRSLAHSGIAIVMVEHIMQAVTELCEEVIVLAFGEKIAH
                     GVPREVMNDARVVEAYLGGTDE"
     gene            complement(1041..2021)
                     /locus_tag="PKN52_05135"
     CDS             complement(1041..2021)
                     /locus_tag="PKN52_05135"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNQ99362.1"
                     /translation="MTAKPWLERGAAPWLGLAAVAAAGAAWYVVFPSSLSLGIGLLLL
                     AGWASAWDLLGGWTGQTSLGHAAFVGLGAYVVGVTATKYGLAPWWSVLLAMSLAAVLA
                     LLWGRITFGLRGSYFVLSSIAVAEILRLVAINERKLTGGAIGLFIFDLDKPFGLDLFS
                     RRTEFWLALGYLLLVLAVVLLVTRGKLGYQMRAVREDEASAQAAGINPVSVKSLAFML
                     SAALTALGGCIYGIFLSALQPQPLFELHLSVRIALVAIIGGRGTLFGPLIGAAMLTLA
                     GELFRNAFAEANLLIYGLLIVIVVLFVPRGLMGELRRRAVRRRYAKSAGG"
     gene            complement(2018..>2382)
                     /locus_tag="PKN52_05140"
     CDS             complement(2018..>2382)
                     /locus_tag="PKN52_05140"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNQ99363.1"
                     /translation="TAQNRLGAELVGINTPRIHALVFGLGMALAMCAGVILAPLLFAV
                     PTVGSSYTLKAFVVTVLGGLGSVPGAIGGGLLLGVVEFLGASYLSSGYRDAYGLFAFL
                     LILLLRPEGLFGRTVKRV"
BASE COUNT          409 a          794 c          872 g          307 t
ORIGIN      
        1 ctcctcgacc gtcatgtcgc caaagagctg ccgccccatg gggacgtgcg cgaggccgcg
       61 cagcgccaga tcgtgaggct tgaggccggt gacgtcctcg ccggccagga agaccttgcc
      121 gccccgcggc ttgatcatgc cggagacggc gcggaggatg gtcgacttgc cactgccgtt
      181 gccgcccacc aacccgacga gttcgccggc gttcacatgc agcgagacgc cgaagacgac
      241 ctgcaactcg ccgtagccga ggtcggcgtc gaccacgtcg agcacgcgcg ccggctgcgc
      301 ctcggctggc tgtgccccga gtcggctcga ggcgccttgc tcactcatcg gtgcctccca
      361 ggtaggcctc gacgacccgt gcgtcgttca tgacttcccg cggtaccccg tgcgcgatct
      421 tctcgccgaa cgcgagcacg atgacctcct cgcagagctc ggtcacggcc tgcatgatgt
      481 gttccaccat gacgatggcg atgccggagt gagcgagcga gcgcacgaag ccgatcatct
      541 ccttgagcgc cggcgggttg agaccggcca tcagctcgtc gaggaacagc acgcgcggct
      601 gcacggcgag cgccttcgcc agttctaggc gccggcgcct ggcgaggttg aggtcgctgg
      661 agggttggac ggcccggtcg ctcaggccga cgagctcgag ggcgtgcctg gcggccgcct
      721 cggcctccga ccgggtggcg tggtggagga aggctgggat gagcacgttc tcgagcacgg
      781 tgagcccctc gaaggggcgc tcgatctgga acgtgcggcc catgccgcgg cgcgtccgcg
      841 cgtagggcgg caggcgctcc acgttcgcgc cgaagagctt gaccgtcccc cgcttggtct
      901 tggcgtagcc ggtcaacgcg ttgaacagcg tcgacttgcc ggcgccgttc ggtccgatca
      961 gaccgacgat ctgccccgcg tcgagggcga gagacacgtc gttgacggcc acgaggccgc
     1021 cgaactggac cgtgatgtgc tcagcctcca gcgctcttgg catagcgcct cctgaccgcg
     1081 cgcctgcgca gctcgcccat gagcccgcgc ggcacgaaga gcacgacgat cacgatgagc
     1141 agaccgtaga tgagtaggtt ggcctccgcg aacgcgttgc ggaagagctc gccggccagc
     1201 gtgagcatgg cggcgccgat gagcggcccg aagagcgtgc cgcggccgcc gatgatggcc
     1261 acgagggcga tgcgcacgct caagtgcagc tcgaacagcg gctgcggctg cagcgccgag
     1321 aggaagatcc cgtagatgca cccaccgagg gccgtgagcg cggccgagag catgaacgcc
     1381 aacgacttga ccgagacggg gttgatgccg gccgcctggg cagacgcctc gtcctcgcgc
     1441 accgcccgca tctggtagcc gagcttgccg cgggtgacga gcaacacgac ggcgagcacg
     1501 agcaggaggt agccgagggc cagccagaac tccgtgcgcc ggctgaacag gtcgagaccg
     1561 aacggcttgt cgaggtcgaa gatgaagagc ccgatggcgc cgccggtcag cttgcgctcg
     1621 ttgatggcga cgagcctgag gatctcggcg acggcgatgc tggagaggac gaagtacgag
     1681 ccgcgcaggc cgaacgtgat gcgcccccac aggagcgcga ggaccgcggc caaggacatg
     1741 gcgagtagga cgctccacca gggggcgagg ccgtacttcg tggcggtgac gccgacgacg
     1801 taggcgccga ggccgacgaa ggcggcgtgg ccgaggctcg tctggcccgt ccagccgccg
     1861 aggaggtccc aggcgctcgc ccagcctgcg agcagcagca agccgatgcc gagggagagc
     1921 gagctcggga agacgacgta ccaggcggcg ccggccgccg ccacggcggc caaccccaac
     1981 caaggcgccg cgccgcgctc gagccacggc ttcgccgtca tacgcgcttc accgtgcgcc
     2041 cgaagaggcc ctctggcctc agcaggagga tgagcaggaa ggcgaagagc ccgtaggcgt
     2101 cgcggtagcc ggacgagagg taggacgcgc cgaggaactc gacgacgccg agcagcaagc
     2161 cgccgccgat ggcgccgggt acgctgccga ggccgcctag cacggtcacc acgaacgcct
     2221 tgagggtgta gctggagccg accgtcggca cggcgaatag gagtggcgcg aggatcacgc
     2281 cggcgcacat ggcgagggcc atgccaagcc cgaagacgag cgcgtggatg cgcggggtgt
     2341 tgatgccgac gagttcggcg ccgaggcggt tctgggccgt gg
//
LOCUS       DAOQYE010000260         2380 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_50068_length_2380_cov_3.221075, whole genome shotgun sequence.
ACCESSION   DAOQYE010000260 DAOQYE010000000
VERSION     DAOQYE010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50068_length_2380_cov_3.221075"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="PKN52_05145"
     CDS             <1..431
                     /locus_tag="PKN52_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HNQ99364.1"
                     /translation="GDQKVYLDLSFFEELTRRYAAPGDFAQAYVVAHELGHHVQNVLG
                     TLSRAQSQGLSVPVELQADCYAGVWGASADRRGIVERGDLQEGLTAAAAVGDDTLAKR
                     AGRIPNQETFTHGSSAQRTQWFTTGFQSGDPNACNTFGSR"
     gene            645..1931
                     /locus_tag="PKN52_05150"
     CDS             645..1931
                     /locus_tag="PKN52_05150"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNQ99365.1"
                     /translation="MLNRFARRLALMLMAAVLVGGSAIAQTEIVYWTHEDPNRTALEE
                     ELIARFEADYPQYKVVRVTNPTDQMAQLILTAFSANRGPDIFNMDIPNEYPYIVNGRV
                     APVNPAAAGYIDEQSIYDAYLPGTLDPVTFEGELYGLPLEVTNWSIYLNMNVFRDAGL
                     DPLTDYPKTWEQLMEVAEKLTIRDGEIITRRGFDFRYPYYLNFLPSMAAQLGGKLIGD
                     DGKSAIVGDEAWLRVLQYFKDWGTTGKNLGSPTYKAARSIFNFDNNDIGMMESGQYQA
                     ARILNDNPGFLPGEGWMVIPFPQWEDAVADVAEAYYGHYWMVNAQASKEKQQAAWTLL
                     GYFASHADEFFQRVAVLQPTQELLNSDMFKNAPYSDVFMGDLAKAPVIYYDENSPQVL
                     QVLKDSIEAVMLQGKEPADVLKTMREGVERALRGEY"
     gene            1942..>2380
                     /locus_tag="PKN52_05155"
     CDS             1942..>2380
                     /locus_tag="PKN52_05155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNQ99366.1"
                     /translation="MARKTVKLTVATRRAMWGWVFVLPALIYFAVFSFYPIFNAFWLS
                     LHTKNPLSLRPPRFVGLRNYLDLLDSATFWNSWKATGLFTLGTAIPLVVLSLLLATII
                     VSRLNFRRFFQLAFYAPAVIPSVVAAAVWLLLLDPRGIANQWVN"
BASE COUNT          423 a          842 c          707 g          408 t
ORIGIN      
        1 ccggcgacca gaaggtctac ctagacctct cgttcttcga ggaactgacc aggcgctacg
       61 cggcgccggg cgacttcgcc caggcgtacg tggtggcgca cgagctgggt caccacgtcc
      121 agaacgtcct cggcacgctc tcgcgcgccc agtcgcaagg cctctccgtg cccgtggagt
      181 tgcaggccga ctgctacgcg ggcgtgtggg gcgcgagcgc cgacaggcgc ggcatcgtgg
      241 agcgcggcga cctacaggag gggttgaccg ccgcggccgc cgtcggcgac gacaccctgg
      301 ccaagcgcgc cggacgcatc ccgaaccagg agacgttcac gcacggcagc tcagcgcagc
      361 gcacccagtg gttcacgacg ggcttccagt ccggcgaccc caacgcctgc aacacgttcg
      421 ggagccgctg accgcgacgc cggatcgggt cacggccgca tgggcggtaa gttcacatga
      481 tcctcatgtt cgttgacggc caccgccttc gctaatatgc tgcacgttga tatcgcgatt
      541 gggtgggagc gcgcacttgc acgggctctc ggacgcctgt cacgcaggtc gcgctgctgg
      601 ttggatatcg cgcccggatc ttcagttggg gagttggagg aatgatgctc aaccgcttcg
      661 ctcgcaggtt ggccctgatg ctgatggccg cagtcctcgt cggaggcagc gccatcgctc
      721 agaccgagat cgtctactgg actcacgagg acccgaaccg cacggccctc gaagaggaac
      781 ttatcgctcg cttcgaggcc gactacccgc agtacaaggt cgtgcgcgtc accaacccga
      841 cggaccagat ggcgcagctc atcctgaccg ccttctccgc caaccgcggc cccgacatct
      901 tcaacatgga catcccgaac gagtacccgt acatcgtgaa cgggcgtgtc gcgccagtga
      961 accctgccgc agccggctac atcgacgagc agtccatcta cgacgcctac ctgcccggca
     1021 ccctcgaccc cgtcactttc gagggcgagc tctacggcct gcccctcgag gtcacgaact
     1081 ggtcgatcta cctgaacatg aacgtgttcc gtgacgccgg cctcgacccc ctcaccgact
     1141 acccgaagac ttgggaacag ctcatggaag tggccgagaa gctcacgatc cgcgacggcg
     1201 agatcatcac gcgccgcggc ttcgacttcc gctaccccta ctacctcaac ttcctgccgt
     1261 ccatggcggc gcagctcggc ggcaagctca tcggcgacga cggcaagagc gccatcgtcg
     1321 gcgacgaggc gtggctccgc gtcctccagt acttcaagga ctggggcacc acgggcaaga
     1381 accttggctc gcccacctac aaggcggcgc ggtcgatctt caacttcgac aacaacgaca
     1441 tcggcatgat ggagtccggc cagtaccagg ccgcccgcat cctcaacgac aaccccggct
     1501 tcctgcccgg cgagggctgg atggtcatcc cgttcccgca gtgggaagac gccgtcgccg
     1561 acgtggcaga ggcctactac ggccactact ggatggtcaa cgcccaggct tcaaaggaga
     1621 agcagcaggc cgcctggacg ctgctcggct acttcgccag ccacgccgac gagttcttcc
     1681 agcgcgtagc ggtcctgcag cctacgcagg agctcctcaa ctccgacatg ttcaagaacg
     1741 ccccgtactc cgacgtcttc atgggcgacc tggccaaggc tcccgtcatc tactacgacg
     1801 agaactcgcc acaggtgctc caggtcctga aggactcgat cgaggcggtc atgctgcagg
     1861 gcaaggagcc tgcggacgta ctcaagacca tgcgcgaagg cgtcgagcgc gccctgcgcg
     1921 gcgagtactg agtaactcga ggtggcccgt aagactgtca aactgaccgt ggccaccagg
     1981 agggcgatgt ggggctgggt gttcgtactc ccagccctca tctatttcgc ggtcttcagc
     2041 ttctacccta tcttcaacgc tttctggctc agcctgcaca ccaagaaccc cttgtcgctg
     2101 cggccaccgc ggttcgttgg ccttcggaac tacctggacc tgctcgactc ggccacgttc
     2161 tggaactcct ggaaggccac cgggctcttc acgctcggca cggccatccc cctcgtggtg
     2221 ctcagcctgc tgctggccac gatcatcgtc tcccgcctca acttccggcg cttcttccag
     2281 ctcgcgttct acgccccggc ggtcatcccg agcgtggtcg ccgcggccgt gtggctgctc
     2341 ctgctcgatc cgcgcggcat agccaaccag tgggtcaact
//
LOCUS       DAOQYE010000261         2379 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_50106_length_2379_cov_2.821859, whole genome shotgun sequence.
ACCESSION   DAOQYE010000261 DAOQYE010000000
VERSION     DAOQYE010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2379
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50106_length_2379_cov_2.821859"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1027)
                     /locus_tag="PKN52_05160"
     CDS             complement(<1..1027)
                     /locus_tag="PKN52_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99367.1"
                     /translation="MSSLTRSFPFATSRRVNRPGRLDAAPNVFGDHPFGAFRLSVSRL
                     PDGGVALAVVAAADRQRVVFSSPPGRAFLAAALGRYAATGRAGHLRPAETIEKRYRHQ
                     LLTAVSSESASERPALELRGRLTAPGASDSVTYSVRLVDVDGERVGVDVELPRPDAAA
                     DILFTDLFWAQDPAARVFGFGAQFTHLDMRGRAVPILTAEQGVGRGAQPLTRYTEAFG
                     GAGGDWWSTYAPVPGFVTTALHGVMLRGTAPALFDLRRPGVAGVRVYAPGLNALFYAT
                     TAPTELLELHTRETGRMPPLPDWAHAGAVVGMQGGGEKVERVVTELAAAGTPLAGVWL
                     QDWVGNRK"
     gene            complement(1057..1227)
                     /locus_tag="PKN52_05165"
     CDS             complement(1057..1227)
                     /locus_tag="PKN52_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99368.1"
                     /translation="MNRAFSVLLLVCFAVTGTYLFTRALNGAGPLAYFFSAVAFGMAI
                     AVVQSLMRGGRR"
     gene            1430..>2379
                     /locus_tag="PKN52_05170"
     CDS             1430..>2379
                     /locus_tag="PKN52_05170"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HNQ99369.1"
                     /translation="MTKRIGFLSFGHWGHSPYSQTRTASDTLLQSIDLAVAAEELGAD
                     GAYFRVHHFARQLGSPFPLLAAVGARTKRIEIGTAVIDMRYENPYYMVEDAGAADLIA
                     GGRLQLGISRGSPEQVVDGWRHFGYAPAEGETDADMARRHATVFFELLKGEGFARPSP
                     RPMFPNPPGLLRLEPHSEGLRERIWWGSATNGTAVWAAELGMNLQSSTLKFDENGKPF
                     HVQQAEQIRAFREAWQAAGHEREPRVSVSRSIFALVDDRDRAYFGRGNEEEDKIGYID
                     EQTRAIFGRSYAAAPDELVKQLAADEAIAEADTLLLTVPN"
BASE COUNT          378 a          839 c          836 g          326 t
ORIGIN      
        1 ccttgcggtt gccgacccag tcctggagcc atacgcccgc cagcggcgtc ccggccgccg
       61 ccagctccgt cacgacgcgc tcgaccttct cgccgccgcc ctgcatgccg accacggcgc
      121 cggcatgcgc ccagtccggc aacggcggca tgcggcccgt ctcgcgcgtg tgcagctcga
      181 gaagttcggt gggagcggtc gtggcgtaga agagggcgtt caggccgggc gcgtacaccc
      241 tcacgccggc caccccgggc cggcgcaggt cgaagagcgc aggggccgtg ccacgcagca
      301 tgacaccgtg gagcgcggtc gtcacgaagc cgggcaccgg cgcgtaggtg ctccaccagt
      361 cgccacccgc gcccccgaag gcctccgtgt agcgcgtgag cggctgggcg ccgcgcccaa
      421 ccccctgctc ggcggtgagg atgggcacgg ccctgccgcg catgtcgagg tgcgtgaact
      481 gcgcgccgaa gccgaagacg cgcgcggccg ggtcctgcgc ccagaagagg tcggtgaaga
      541 ggatgtcggc ggcggcgtcc ggtcgcggca actccacgtc gaccccgacc cgctccccgt
      601 cgacgtccac gaggcgcacg gagtacgtca cgctgtcgga cgcgcccggg gcggtcaggc
      661 gccctctcaa ctcgagcgcc ggtcgctcgg aagcgctttc cgagctgacg gccgtcagga
      721 gttggtggcg gtagcgcttc tcgatcgtct cagcgggtcg gagatggccc gcccgcccgg
      781 tggcggcgta ccgacccagc gcggcggcaa ggaaggcgcg gccggggggc gaggagaaca
      841 cgacgcgctg ccggtcggcc gcggcgacca ccgccagcgc cacgccgcca tccgggagcc
      901 tcgagacgct tagcctgaac gccccgaagg ggtggtcgcc gaagacgttc ggcgcggcgt
      961 cgagccggcc gggccggttg acgcgcctgc tggtcgcgaa cgggaaggaa cgcgtgaggg
     1021 acgacatcgt tgcctagcgg ccccgtcagc gcgtgctcag cgccgcccgc cgcgcatgag
     1081 gctctgcacg acggcgatgg ccatgccgaa agcgaccgcg gagaagaagt aggcgagcgg
     1141 tcccgctccg ttcagcgcgc gcgtgaacag gtaggttccg gtgacggcga agcaaacgag
     1201 cagcagcacg ctgaacgcgc ggttcatccg ggtggccccg cctcgtctgg tcgtccgccg
     1261 gctcgcatct tgagcgaagc tatcacgtcg agggcgcgtg gctctacgcc ggctcacgca
     1321 tgcagcgcga tcaccgtgcg acaccatgcc cggggtatcg gagcaccggt ccttatggaa
     1381 gccaaccgac gacatcggca gtgcccccgg cgtgccagac tcgcttcaca tgacgaagcg
     1441 catcggattc ctctcgttcg gtcattgggg gcactcgccg tactcgcaga cacgcacggc
     1501 cagtgacacg ttgctacagt cgatagatct ggccgtcgcc gcggaggaac tcggggcaga
     1561 cggggcctac ttccgcgtgc accacttcgc gcgccagctc ggttcgcctt tccccctcct
     1621 ggccgccgtc ggggcgcgca cgaagcgcat cgagatcggc acggccgtta tcgacatgcg
     1681 ttacgagaac ccctactaca tggtcgagga cgccggggcg gccgacctca tcgcgggtgg
     1741 ccgcctgcag ctcggcatca gccgcggctc gccagagcag gtcgtggacg gttggcgaca
     1801 cttcggttac gcgcccgcgg agggtgagac ggatgccgac atggcgcgac ggcacgccac
     1861 cgtgttcttc gagctgctga agggcgaggg cttcgcgcgt ccgagtccgc gacccatgtt
     1921 cccgaacccg cccggcctgc tgcgcctcga accgcactcg gagggtctgc gcgagcgcat
     1981 ctggtggggc tccgccacca acggcactgc cgtctgggct gcggaactcg gcatgaacct
     2041 ccagagctcc accttgaagt tcgacgagaa cggcaagccg ttccacgtgc agcaggccga
     2101 gcagatccgc gccttccgcg aggcgtggca ggcggccggg catgagcgcg agccgcgcgt
     2161 gtccgtgagc cgcagcatct tcgcgctggt cgatgaccgc gaccgcgcct atttcggccg
     2221 cggcaacgag gaggaagaca agatcggcta tatcgacgaa cagacgcgcg cgattttcgg
     2281 ccggagctat gctgccgcgc ctgacgaact ggtgaagcaa ctcgctgccg acgaagcgat
     2341 cgcggaagcc gatacgctcc tgctaacggt gcccaacca
//
LOCUS       DAOQYE010000262         2373 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_50282_length_2373_cov_4.114323, whole genome shotgun sequence.
ACCESSION   DAOQYE010000262 DAOQYE010000000
VERSION     DAOQYE010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50282_length_2373_cov_4.114323"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..773)
                     /locus_tag="PKN52_05175"
     CDS             complement(<1..773)
                     /locus_tag="PKN52_05175"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNQ99370.1"
                     /translation="MSDKHFVAVIMAGGQGQRFWPLSTADRPKQFLDISRSGRTLLQA
                     TYDRVLPLAGSPERVIIGTAERYVPLVREQLPEIPPENLVVEPTPRDSAPAIALACLR
                     IQERFGDVVAGFFSSDHEIGDSNAFQRVARTAIELADDEAGLVTIGITPARPATGYGY
                     IEVGTPVGGGFRVARFVEKPNLRTAQAYVASGRYLWNAGIFVWRISSALSELDRHVPE
                     LMAPLRSALEAREAEAVFPTLKKISIDGHTDSDGSDAYN"
     gene            complement(804..1634)
                     /locus_tag="PKN52_05180"
     CDS             complement(804..1634)
                     /locus_tag="PKN52_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HNQ99371.1"
                     /translation="MPGKTASNGALRAFGFVTAATRPLYWLYERRLARAVKAAGHLPR
                     HIGIIMDGNRRFAKAAGIDVKAGHDYGASKAREVLDWCLELGIPHVTLWGFSTENRSR
                     SAEEVGHLHRLFAEQARSLLNDERLHANRVKVRIIGDIADFPDEAKAALAAMEEATRD
                     YDGMHLNVALGYGGREEIVDAVKRLATERAAEGATLTDLASSLTADQITHHLYTAGLP
                     DPDFVIRTSGEVRLSGFLLWQTAYSEFYFCDANWPEFRRVDFLRAVRSFQARERRFGK
                     "
     gene            complement(1654..>2373)
                     /locus_tag="PKN52_05185"
     CDS             complement(1654..>2373)
                     /locus_tag="PKN52_05185"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HNQ99372.1"
                     /translation="KYVQYLPDLRGLPTNSVVAAPLGTALGLEVRNQLASSANRAVKR
                     VVDFLGAGLLLLVLGVPLLIVALLIRLDSRGPALYFSPRIGRYGKTFGCIKFRTMHVD
                     ADERLQTLLSEHPELREEYARFHKLERDPRVTRVGRVLRRVSFDELPQLLNVVRGQMS
                     LVGPRPYMEREREEMGKDQDIIFLARPGMTGYWQTEARNDVSFDERQAMEAHYVRNWS
                     VWWDIDILLRTPAAMLDKRGK"
BASE COUNT          372 a          762 c          835 g          404 t
ORIGIN      
        1 tggttgtagg cgtccgagcc gtccgaatcc gtgtggccgt cgatgctgat cttcttgagg
       61 gttgggaaga cggcctcggc ctctcgggct tccagagcgc tccgtaacgg cgccatcaac
      121 tccggcacgt ggcggtcgag ctccgagagc gcgctgctga tccgccacac gaatatgccg
      181 gcgttccata agtacctgcc ggatgccacg taagcctggg ccgttcgcag gttgggcttc
      241 tcgacgaagc gcgccacccg gaagccgccg cccacgggtg tcccgacctc gatgtagccg
      301 tagcctgtcg ccgggcgcgc cggcgtgatg ccgatggtca cgagccccgc ctcgtcgtcg
      361 gccagctcga tggccgtgcg agcgactctt tggaaagcgt tggagtcgcc gatctcgtga
      421 tcggaagaga agaagcccgc taccacgtcg ccgaagcgtt cttggatccg gaggcacgcc
      481 agggcgatgg ccggtgcaga gtcgcgcggt gtcggctcca cgacaaggtt ctcgggcggt
      541 atctcgggta gctgctcacg cacaaggggc acgtagcgtt ccgcggtgcc tatgatcact
      601 cgctccgggg agccagccag gggcagcacc cggtcgtagg tggcttgaag tagcgtccgt
      661 ccggaacggc tgatgtcgag gaactgcttg ggtcgatcgg cggtggagag aggccagaag
      721 cgctgacctt ggcccccggc catgatcacg gctacgaaat gcttgtcgct cacatcggtc
      781 cccccttggc ggctaccccg ccctcacttc ccgaaccggc gctcgcgcgc ttggaacgac
      841 ctgacggcgc gtaggaaatc gacccggcga aactccggcc agttggcgtc gcagaagtag
      901 aactcgctgt acgcggtctg ccacaggagg aagccggaga ggcgtacctc cccggaggtg
      961 cggatcacga agtccgggtc cggcaggccg gccgtgtaga gatggtgggt gatctgatct
     1021 gcggtaaggc tcgaggccaa gtcggtcagc gtggcaccct cggccgcccg ttccgtggcc
     1081 aggcgcttca ccgcgtcaac gatctcctcg cgcccaccgt aaccgagggc aacgttgagg
     1141 tgcatgccgt cgtagtcgcg ggtggcttct tccatggcgg cgagtgcggc cttggcctcg
     1201 tcggggaagt cggcgatgtc gccgatgatg cggaccttca cgcggttcgc gtgcaggcgc
     1261 tcgtcgttca gtaggctgcg cgcctgctcc gcgaagagac gatgcagatg gcccacctct
     1321 tccgccgagc gggaacggtt ctccgtgctg aacccccaca aggtcacgtg agggatgccg
     1381 agttcgaggc accagtcgag cacctctcga gccttgctcg ctccgtagtc atgcccggcc
     1441 ttgacgtcga tgccggccgc cttggcgaag cgccggttgc cgtccatgat gatgccgatg
     1501 tggcgcggga ggtgccccgc ggccttcacg gcgcgcgcca ggcgccgctc gtacagccag
     1561 tagagcggcc tcgtcgcggc cgtcacgaaa ccgaaggcgc gtagggcccc gttggaggcg
     1621 gttttcccag gcacggcgta ctctaccgca gccctacttg cccctcttgt cgagcatggc
     1681 cgccggcgtg cggaggagga tgtcgatgtc ccaccacacc gaccagttgc gcacgtagtg
     1741 cgcctccatg gcctggcgct cgtcgaagct gacgtcgttc cgcgcctccg tctgccaata
     1801 gccggtcatg ccgggacggg cgaggaagat gatgtcctgg tccttgccca tctcctcgcg
     1861 ctcgcgctcc atgtacggcc gcgggccgac gaggctcatc tgaccgcgta cgacgttgag
     1921 gagttgcggc agctcgtcga aggacacgcg ccgaaggacg cgcccgacgc gcgtcacgcg
     1981 cgggtcgcgc tcgagcttgt ggaaccgcgc gtactcctcg cgtagttccg ggtgttcgct
     2041 caggagggtc tggagccgct catcggcatc cacgtgcatg gtacggaact tgatgcagcc
     2101 gaaggtcttg ccgtagcgcc cgatgcgcgg cgagaagtac agggccgggc cgcgcgagtc
     2161 gaggcggatg aggagcgcga cgatgagtag gggcacgccg agcacgagca ggaggaggcc
     2221 ggcgccgagg aagtcgacga cgcgcttcac ggcgcggttc gcgctggacg cgagctggtt
     2281 gcgcacttcg agcccgaggg cggtgccgag gggggccgcc accacggagt tggtaggtag
     2341 tccgcgcagg tcggggaggt actggacgta ctt
//
LOCUS       DAOQYE010000263         2369 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_50448_length_2369_cov_2.463699, whole genome shotgun sequence.
ACCESSION   DAOQYE010000263 DAOQYE010000000
VERSION     DAOQYE010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50448_length_2369_cov_2.463699"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..819)
                     /locus_tag="PKN52_05190"
     CDS             complement(<1..819)
                     /locus_tag="PKN52_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HNQ99373.1"
                     /translation="MTDDLRARAATFGLTPEEFDEVVLRLGRQPNALEAAMFGALWSE
                     HCGYKNSKPLLRRLPTKGPQVIQGPGENAGVVDIGEGWGVAFKMESHNHPSAVEPVQG
                     AATGVGGILRDIFAMGARPFATLNALRFGELGEERNRYLLSGVVHGIALYGNAIGVPT
                     VGGEVEVNACFGENPLVNVMALGLLRHDELQKGTVGAPGNRLFYVGSKTGRDGLGGAV
                     FASADLSGSSSADRPAVQVGDPFMEKLLLEACLEAYGAGLVAGVQDMGAAGLTSS"
     gene            complement(886..1623)
                     /gene="purQ"
                     /locus_tag="PKN52_05195"
     CDS             complement(886..1623)
                     /gene="purQ"
                     /locus_tag="PKN52_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HNQ99374.1"
                     /translation="MRVAVVTFPGSNCDMDAVHALGVLGAHVWTTWHANDTLTSAGRA
                     PDAVVIPGGFSYGDYLRCGALAARSPVMGAVRAFATAGGPVIGICNGFQVLTEAGLLP
                     GQLVRNAELEFRCEDVHLRVERSDLPFTRRARAGQVLRLPIAHAEGRYFADRETVDRL
                     RGEGRVVFRYVDATGEQTSEANVNGSVDAIAGVVNERGNVLGMMPHPERAVEALLMDD
                     RRSGSDGGREDGRLILGSLLDAALHAR"
     gene            1737..1820
                     /locus_tag="PKN52_05200"
     tRNA            1737..1820
                     /locus_tag="PKN52_05200"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1770..1772,aa:Leu,seq:caa)
     gene            1883..2113
                     /gene="secG"
                     /locus_tag="PKN52_05205"
     CDS             1883..2113
                     /gene="secG"
                     /locus_tag="PKN52_05205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HNQ99375.1"
                     /translation="MFWIAFVIFCVVSVVLTVAVLMQEPKQSGLGDALGGGGGGDFGA
                     SLGGTAGGLHRTTIYLAIAWAVMALLLGLIPR"
BASE COUNT          361 a          811 c          798 g          399 t
ORIGIN      
        1 ggaactcgtg aggcctgcgg cgcccatgtc ctgcacgccg gccacgagac cggcgccgta
       61 cgcctcgagg cacgcctcga gcagcagctt ctccatgaac gggtcgccga cctggacggc
      121 ggggcggtcg gcgctggaac tcccggagag gtcggccgag gcgaacacgg cccccccgag
      181 accgtcacgg cccgtcttgg agccgacgta gaagaggcgg ttacctggag cgccgaccgt
      241 gcccttctgc aactcatcgt gcctcagcag tcctaacgcc atgacgttca cgagcgggtt
      301 ctcgccgaag catgcgttga cctcgacctc gccgcccacc gtcggcacgc ctatagcgtt
      361 gccgtacagc gcgatgccgt ggaccacgcc gctcaacagg tagcggttgc gttcctcgcc
      421 gagttcgccg aagcgcaggg cgttcagggt agcgaacggg cgcgcgccca tcgcgaagat
      481 gtcgcggagg atgccgccaa cgccggtggc ggcgccctgg acgggctcga cggcgctcgg
      541 atggttgtgg ctctccatct tgaaggcgac gccccagccc tcgcctatgt cgaccacgcc
      601 ggcgttctcg ccgggaccct ggatgacctg gggccccttg gtcggaaggc ggcgcaggag
      661 aggcttggag ttcttgtagc cgcagtgttc gctccacaac gcgccgaaca tggccgcctc
      721 gagggcgttc ggctgcctac cgagtctcag gacgacttcg tcgaactcct ccggtgtcag
      781 gccgaaggtg gcggcgcgcg ccctcaggtc atcggtcatg gtcggactct ccgttaccgg
      841 tctcgcaagg tctctgttcg cgtcgacgct cgcggtcggc gccgatcatc tggcgtgcag
      901 tgcggcgtca aggagggagc ctaggatgag ccgaccgtcc tcacgaccgc cgtcgctccc
      961 gctcctgcgg tcgtccatga gcagcgcctc gacggcgcgc tcggggtggg gcatcatgcc
     1021 gaggacgttg ccgcgttcgt tgaccacgcc ggcgatggcg tccacgctgc cgttcacgtt
     1081 ggcctccgag gtctgctcgc ccgtcgcgtc gacgtacctg aagacgacgc ggccctcgcc
     1141 gcgcagccgg tcgacggtct cacggtcggc gaagtagcgg ccctcagcgt gcgctatggg
     1201 caggcgcagc acctgcccgg cgcgcgcgcg acgtgtgaac gggaggtcgg agcgctcgac
     1261 gcgcaggtga acgtcctcgc acctgaactc cagctcggcg ttcctgacca gttgccccgg
     1321 gagcaggccg gcctccgtga ggacctggaa accgttgcag ataccgatca ccgggccgcc
     1381 ggcggtggcg aaggcgcgga ccgcgcccat gacggggctc cgggcggcga gcgcgccgca
     1441 ccgcaggtag tcgccgtagc tgaagccgcc ggggatgacc acggcgtccg gcgctcggcc
     1501 ggcgctcgtc agggtgtcgt tcgcatgcca ggtcgtccag acatgcgcgc cgagcacccc
     1561 cagggcgtgg accgcgtcca tgtcgcagtt gctgcctggg aaggtcacga ccgccacgcg
     1621 catctctaac ctgtaagcta ccatgtcgtc caagctggta acttgccccg ccgggcgccg
     1681 gattggtagt atcctcaacc gtccgcgcgc tatggcaacg tcgcagcgag cgaggcgccg
     1741 agatggcgga acggtagacg cagttgactc aaaatcaacc ggggcgaccc gtgcgggttc
     1801 gagtcccgct ctcggcacca aacggttctc aaggatagcg accagcccgc cgttcgttaa
     1861 gcgggcgtcg actggaggaa tgatgttctg gatcgcgttc gtaatcttct gcgtcgtctc
     1921 ggtggtactc accgttgcgg tgctcatgca ggagcccaag cagtctggcc tgggtgacgc
     1981 gctgggcggc ggcggtggcg gcgacttcgg cgcgagcctc ggcggcacgg ccggcggcct
     2041 gcaccgcacg accatctacc tggccattgc ctgggccgtc atggcgctcc tcctcggact
     2101 gatcccgcgc tgagcacgcg cactgccctt tcctcaagga accgtgaggc tttcaccctc
     2161 ctcaccttac ttgcggcatc ggcgttttga ggttaccgta aaccgtcgta aactttgggc
     2221 gtgacggcgc aggtccgatc ccggtccggt gcccacgcca accgtagttc aatggaggag
     2281 aaatgaagag actactcacc gctattagcc ttatggcgat cgcgaccatc gcgttcgcgc
     2341 agccgttcgt ctggccggac gcctggtcc
//
LOCUS       DAOQYE010000264         2367 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_50493_length_2367_cov_3.205017, whole genome shotgun sequence.
ACCESSION   DAOQYE010000264 DAOQYE010000000
VERSION     DAOQYE010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50493_length_2367_cov_3.205017"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="PKN52_05210"
     CDS             complement(<1..265)
                     /locus_tag="PKN52_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99376.1"
                     /translation="MSLSRTARILLALLLVAAAAFVWVSYLRNPQPQTTLVNTPTVVP
                     GAAAADAATNSPTATAAPLVGTDAVGEASSNGATATAGPDGQAA"
     gene            complement(269..853)
                     /gene="pilO"
                     /locus_tag="PKN52_05215"
     CDS             complement(269..853)
                     /gene="pilO"
                     /locus_tag="PKN52_05215"
                     /inference="COORDINATES: protein motif:HMM:NF016253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HNQ99377.1"
                     /translation="MSRSFSLSKLKQRDWAIICIVLSLVAAVVWYFYMYSPTQDRIAQ
                     LESDITRLDADIRRGEDARRNLPTLRLAVAELEADRRDFLTQLPRESDIAGLIDSLRA
                     NAQESDVVVRSFSQGSAQESIQDVRPIGFSIDTQGTYAETMDYLARLEAMRRFAKVGR
                     VSLDIEDNSSSDPNLNASFAFTVYVYTGSDPGEQ"
     gene            complement(850..1458)
                     /locus_tag="PKN52_05220"
     CDS             complement(850..1458)
                     /locus_tag="PKN52_05220"
                     /inference="COORDINATES: protein motif:HMM:NF037069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99378.1"
                     /translation="MININLLPKNLRRVREPGYWRLLAVAFPLLVLVVAGIMQYTANA
                     TAANLQREKLAREDQLALLQPFLREQRDLVSRQQQLNQLIAVAQSVRENTIVWTGEIA
                     GLLENLPVGGDTRPAIDFRSLSMQSVFPPRADAERYEGRPVTAEMSVSGTVTNTEVLA
                     EFIRALERSENYGVAFQNAQRSDEEGVYQYSLTVGSIQEGAR"
     gene            complement(1451..>2367)
                     /gene="pilM"
                     /locus_tag="PKN52_05225"
     CDS             complement(1451..>2367)
                     /gene="pilM"
                     /locus_tag="PKN52_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HNQ99379.1"
                     /translation="AITRNIHVPRMSVAELDEAIRWEAERYIPFPIDEVVLDYFVLDN
                     PEDVAEGEQLEVVIAAARLDEVSQQVDYLKHAGLEPHVIDVKPFSLLRALRGSLHGEH
                     LTKATLSGQNFTEANEIGVVLEIAASTSTVTLVRGQRVLMNRNINVSSDDFTTAIQRA
                     FGLDFDSAEEVKLEYGTATIPTEDEEDLLNFDAKREQFSPSRVYDALRPVIVELTTEI
                     RRSLEFFRVQSGDATISRMLVTGGGAKLRGLPDAIGDALGIKVELGDPWLAVTFDEGR
                     FDAQYLKKIAPEFAVSLGLALRGVAGVD"
BASE COUNT          369 a          754 c          836 g          408 t
ORIGIN      
        1 cggccgcctg gccgtccggg ccggcggtcg cggtcgcgcc gttcgaagag gcctcaccga
       61 ccgcgtctgt gccgaccaac ggggcggcgg tggcggtagg cgagttggtc gccgcgtcgg
      121 ccgcggcggc gcccgggacg acagtaggcg tgttgaccag cgtcgtctgc ggctgcgggt
      181 tcctcaagta gctcacccag acgaacgccg cagcggctac gagcagtagc gccagcagga
      241 tgcgagctgt tctagaaagt gacatggcct attgctcccc tgggtcgcta cccgtgtaga
      301 cgtagacggt gaaagcgaag gaggcgttga ggttcgggtc gctcgacgag ttgtcctcga
      361 tgtcgaggct cacgcggccg accttggcga accggcgcat ggcctcgagg cgcgcgaggt
      421 agtccatggt ctccgcgtac gtgccctggg tgtcgatgct gaacccgatg ggccgcacgt
      481 cctggatgga ctcctgggcg gagccttggc tgaacgacct gaccaccacg tccgactcct
      541 gggcgttggc gcgcagcgag tcgatgagcc cggcgatgtc gctctcgcgc gggagttgcg
      601 tcaggaagtc gcggcggtcg gcctcgagct cggccacggc caacctgagc gtcgggaggt
      661 tgcgccgggc gtcctcgccg cgccggatgt cggcatccaa ccgcgtgatg tctgactcga
      721 gctgggcgat ccggtcttgc gtcgggctgt acatgtagaa gtaccagacg accgccgcga
      781 ctagggagag gacgatgcag atgatggccc agtcgcgctg cttgagcttg ctgagactga
      841 acgacctgct caccgcgctc cctcctggat gctccccacg gtcagcgagt actggtagac
      901 gccttcctca tcggagcgct gcgcgttctg gaacgccacg ccgtagttct cgctccgctc
      961 cagcgcgcgg atgaactcgg cgagcacctc ggtgttggtg accgtgccgg acacgctcat
     1021 ctcggccgtc acgggccgac cctcgtaacg ttccgcgtcc gcccggggcg ggaagacgct
     1081 ctgcatggac agcgagcgga agtcgatggc cgggcgcgtg tcgccgccca cgggcaggtt
     1141 ctcgaggagg cccgcgatct cgcccgtcca tacgatcgtg ttctcgcgca ccgactgggc
     1201 cacggcgatg agctggttga gctgctgctg ccgggagacg aggtcgcgct gctcgcgcag
     1261 gaacggttgg aggagcgcga gctggtcctc gcgggcgagc ttctcgcgct gcaggttcgc
     1321 ggccgtggcg ttggccgtgt actgcatgat gccggcgacc acgagcacga ggagcgggaa
     1381 ggcgacggcc aacagtcgcc agtaaccggg ctcgcgtacg cggcgtaggt tcttgggaag
     1441 taggttgatg ttaatcaacg ccggccaccc ccctcagcgc cagacccaac gagaccgcga
     1501 actcaggagc gatcttcttg aggtactggg cgtcgaaacg cccctcgtcg aacgtgacgg
     1561 ccagccacgg gtcgccgagc tcgaccttga tgccgagggc gtcgccgatg gcgtcgggca
     1621 acccgcgcag cttggcgccc ccgcccgtga cgagcatgcg actgatcgtg gcgtcgcccg
     1681 actgcacgcg gaagaactcg agtgaccggc ggatctcggt ggtcagctcc acgatcaccg
     1741 gccggagcgc gtcgtaaacg cggctgggag agaactgctc gcgcttggcg tcgaagttga
     1801 ggaggtcctc ctcgtcttcc gtcgggatcg tggcggtgcc gtactcgagc ttgacctcct
     1861 ccgcactatc gaaatcgagc ccgaaggcgc gctggatggc ggtcgtgaag tcgtcgctgc
     1921 tgacgttgat gttgcggttc atgagcacgc gctgcccacg cacgagggtg acggtcgagg
     1981 tgctcgccgc tatctcgagg accacgccta tctcgttggc ctcggtgaag ttctggcccg
     2041 acagcgtggc cttcgtgagg tgctcgccgt ggagcgagcc gcgcagcgcc ctgaggagcg
     2101 agaacggctt gacgtcgatg acatgcggct ccaggccggc atgcttcagg tagtcgacct
     2161 gctgcgagac ctcgtcgagg cgcgcggccg cgatgacgac ctcgagctgc tcaccctccg
     2221 cgacgtcctc cgggttatcc agcacgaagt agtcgaggac gacctcgtcg attgggaaag
     2281 ggatgtagcg ctcagcttcc cagcggatcg cctcgtcgag ctccgctacc gacatgcggg
     2341 gcacatggat gttacgggtg atggcct
//
LOCUS       DAOQYE010000265         2362 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_50676_length_2362_cov_3.106199, whole genome shotgun sequence.
ACCESSION   DAOQYE010000265 DAOQYE010000000
VERSION     DAOQYE010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50676_length_2362_cov_3.106199"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            22..885
                     /locus_tag="PKN52_05230"
     CDS             22..885
                     /locus_tag="PKN52_05230"
                     /inference="COORDINATES: protein motif:HMM:NF011630.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese/iron ABC transporter ATP-binding
                     protein"
                     /protein_id="HNQ99380.1"
                     /translation="MTRGSGTTGELGPDAGISVEDVTVTYRNGHTAIRGASFEVPVGT
                     ITALVGVNGAGKSTMFKAIMGFVRPARGEISVLGLSVAKALRQNLVAYVPQAEEVDWN
                     FPVLVEDVVMMGRYGHMGGMRIPRRADREAVGAALERVGMSALTKRQIGELSGGQRKR
                     VFLARALAQDSRVILLDEPFSGVDVKTEERIITLLAELRDEGRVMLVSTHDLASVPTF
                     CDHVVIVKGTVLAYGPTAVTFTKENLELAFGGAVGRAMPDRPFFGALPDIDERAAERR
                     ALSAQAGGSQN"
     gene            885..1745
                     /locus_tag="PKN52_05235"
     CDS             885..1745
                     /locus_tag="PKN52_05235"
                     /inference="COORDINATES: protein motif:HMM:NF013143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HNQ99381.1"
                     /translation="MATLLEPFSYGYMQNAMWVSALVGCVCAFLSCYLMLKGWSLIGD
                     ALSHAIVPGVAGAYMLGLPFSAGAFVAGLLAAGTMLVLNQRTKLKEDVVIGLILVTFF
                     GLGLFMVSVSPVHVNVQTIVLGNILAITPSDTLQLAIIGFVCLGVLILKWRALVLTFF
                     DENHARSVGLRPGRLKVLFFALLAAATVAALQTVGAFLVICMVVTPGATAYLLTDRFP
                     RLLAIAVTIGTVTSFIGAYISYFLDGATGGIIVVLQTAIFLIAFTFAPKHGVLAARRR
                     AAQALRKVAS"
     gene            1748..>2362
                     /locus_tag="PKN52_05240"
     CDS             1748..>2362
                     /locus_tag="PKN52_05240"
                     /inference="COORDINATES: protein motif:HMM:NF013143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HNQ99382.1"
                     /translation="MLETLLAPFRFEFMNEAFLITTLVAVPAALLSCFLVLKGWALLG
                     DAVSHAVFPGVVLAYILSWPLAVGAFLAGMFTAVATGYLGANSRIKQDTVMGVVFAGM
                     FGLGVVMFVKVKTDVHLNHILFGNMLGVSWGDVVQTGVIALVATTIVVLRWRDLLLYV
                     FDAAQARAVGLPVQVLHYGLLGLISLTIVAALQAVGIILSVAILI"
BASE COUNT          349 a          785 c          771 g          457 t
ORIGIN      
        1 tgggagagtg aggaggcagg tatgaccaga ggttccggga cgaccggcga actcggcccc
       61 gacgccggta tcagtgtcga ggacgtgacc gtcacgtacc gcaacggaca caccgccatc
      121 aggggagcga gcttcgaggt gccggtcggg accatcactg cgctcgtagg ggtgaacggc
      181 gcaggcaagt ccacgatgtt caaggccatc atgggcttcg tcaggcccgc acgcggcgag
      241 atcagcgtcc tgggcctcag cgtagcgaag gcgttgcgcc agaacctcgt cgcgtacgtg
      301 ccacaggcgg aggaggtcga ctggaacttc cccgtgctcg tcgaggacgt ggtgatgatg
      361 ggtcggtacg gccatatggg cggcatgcgc atcccgcgcc gcgccgaccg cgaggccgtc
      421 ggtgcggcgc tcgagcgcgt ggggatgagc gcgctgacca agcgccagat cggagagctg
      481 tctggcgggc agcgcaagcg cgtcttcctc gccagggcct tggcccagga cagccgcgtg
      541 atcctgctcg acgagccgtt cagcggggtg gacgtcaaga cggaggagag gatcatcacc
      601 ctcctggcgg agttgcggga cgaggggcgc gtcatgctcg tctcgacgca cgacctagcc
      661 tccgtgccga ctttctgcga ccatgtcgtg atagtcaagg gtaccgtcct tgcctacggg
      721 cctaccgcag tcacgttcac caaggagaac ctcgaactgg cgttcggggg ggcggtcggc
      781 cgagctatgc ccgaccgccc gttcttcggt gcgctgcccg acatcgacga gcgtgccgcc
      841 gagcggcgag ccctcagcgc gcaggcgggc ggctcgcaga actgatggcg actctgctcg
      901 agccgttctc gtacggctac atgcagaacg ccatgtgggt ctctgccctt gtcggctgcg
      961 tgtgcgcgtt cctctcctgc tatctgatgc tcaaggggtg gtcgctcatc ggcgacgcgc
     1021 tctcacacgc gatcgtgccg ggcgtggcgg gggcgtacat gctcgggctg ccgttctccg
     1081 ccggggcgtt cgtggccggg ctgctggcgg ccggcacgat gctcgtgctg aaccaacgca
     1141 cgaagctcaa ggaggacgtg gtcatagggc tcatcctcgt cacgttcttc gggctcggtc
     1201 tcttcatggt gtccgtctcg cccgtgcatg tgaacgtgca gaccatcgtc ttgggcaaca
     1261 tcctcgccat cacgccgtct gacaccctgc aactcgccat catcggcttc gtgtgcctgg
     1321 gtgtcctgat cctcaagtgg cgggctctcg tcctgacctt cttcgacgag aaccacgcgc
     1381 gctccgtagg attgcggccc ggccggctca aggtcctctt cttcgccctc ctcgccgctg
     1441 caacggtggc ggccctgcag acggtcgggg cgttcctagt catctgcatg gtcgtgacgc
     1501 cgggtgccac cgcctacctc ctgacggacc gcttccccag gctgttagcc atagccgtaa
     1561 ccatcggaac cgtgaccagc ttcatcgggg cctacatcag ctacttcctc gacggggcga
     1621 ctggcggcat catcgtcgtg ttgcaaacgg ccatcttcct gatcgctttc acgtttgcgc
     1681 cgaagcatgg cgttctcgcg gcgcgacggc gggcggccca agcgctgcgg aaggtggcgt
     1741 cctgatcatg ctcgagaccc tcctcgcacc cttccgcttc gagttcatga acgaggcctt
     1801 cctcatcacg acactggtgg ccgtgccagc ggcgctgctc tcctgcttcc tcgtcctcaa
     1861 gggctgggcc ctgcttggcg acgcggtcag ccatgccgtc ttccctggcg tagtgctcgc
     1921 gtacatcctg agctggccgc tcgcggtagg cgcgttcctg gccggcatgt tcacggccgt
     1981 cgccacgggc tacctcggtg ccaacagccg tatcaagcag gacaccgtga tgggcgtggt
     2041 gttcgccggt atgttcgggc tcggcgtcgt catgttcgtg aaggtcaaga ccgatgtcca
     2101 cctgaaccac atcctgttcg ggaacatgct cggcgtgagt tggggggacg tcgtccagac
     2161 gggagtcatc gccctggttg cgacgaccat cgtagtactc aggtggcgcg acctcctcct
     2221 gtacgtcttc gacgccgccc aggcgcgcgc cgtcggcctg ccggtgcagg tcctgcacta
     2281 cggactcctt ggcctcatct ccctcaccat cgttgccgcg ctccaagccg tgggcatcat
     2341 cctgtcggtg gccatcctca tc
//
LOCUS       DAOQYE010000266         2362 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_50700_length_2362_cov_1.991331, whole genome shotgun sequence.
ACCESSION   DAOQYE010000266 DAOQYE010000000
VERSION     DAOQYE010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50700_length_2362_cov_1.991331"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1113)
                     /locus_tag="PKN52_05245"
     CDS             complement(<1..1113)
                     /locus_tag="PKN52_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99383.1"
                     /translation="MHSLRGTRGLIGGLLFALAAAVALPTPAVAGPDPAAKRPNTGRY
                     RLFVGNLGAITINRVYYGLNTRGEVGVDSLNSSTIGGGFWPKGTGNQYMFNSGLQVAG
                     IIAGTKPANPWGGDTTGGMFFDASGLRQHGTAVTELYNAVSPVDQAVWPQAAFVPQGD
                     ASEELFDPLLRGRMSASQGDVWFMSSEADPGLNAARPHPLGIIAEYRVMGWNFPAGNE
                     DLLYLIVTFYNITTTNEAAYAQYRPGLREILIEQAQQFHALNNAAFSVDLPDEGYTID
                     PFYAAFSADPDVTSSAGVNFSSVNMPFAMGYAYHRDFLRAAGWTFSPNIFGAPFFAGS
                     GFVGIKYLKSATGPGEIALYSNTTNGGAFPDPNSAVR"
     gene            complement(1194..>2362)
                     /locus_tag="PKN52_05250"
     CDS             complement(1194..>2362)
                     /locus_tag="PKN52_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99384.1"
                     /translation="KFVNGTGTGVYTDLAITNTNNNYGSDLDFGKTILFEFGVRHAFS
                     DDMVLDLTVYNKDNLANAAGRLIGAIDPRTNSRVDLRQTVNADFGNTRGIDLRLDRRF
                     GNIFNVMLAYSYQDARNTGTDPYTYINFGSRITSAITGSAAPPPQAAQPVGFSRPHSL
                     AAQFSFNFPADFSEGSVVGAIMQNMGIFGTARYASGTPYTRCDPTLDDDLAVTSGGTC
                     NNLGGGFNAARLPAFKNVDLRVTKGFRLGALDLTAYADARNVFNIKNILAVFSQTGTT
                     SNARLAATRWTGDSSTFALNAAANGIYNATTGAIDLPDSDSQCGSWVTTGNNPSPPTC
                     YFYRKGEQRYGNGDGIYTLEEQRRASDIAQLGTFHVSRWAGGGRILRFGMEVNF"
BASE COUNT          365 a          717 c          896 g          384 t
ORIGIN      
        1 ccggacggcc gagttcgggt cggggaacgc gccgccgttg gtggtgttcg agtagagcgc
       61 gatctcgccc ggcccggtgg ccgacttgag gtacttgatg cccacgaagc cggagcccgc
      121 gaagaacggg gcgccgaaga tgttgggcga gaaggtccag ccggccgccc gcaggaagtc
      181 gcggtgatag gcgtagccca tcgcgaacgg catgttgacc gacgagaagt tgacgccggc
      241 cgacgaggtg acgtcggggt cggcggagaa cgcggcgtag aacgggtcga tggtgtagcc
      301 ctcgtcgggc aggtccaccg agaacgccgc gttgttgagc gcgtggaact gctgcgcctg
      361 ctcgatcagg atctcgcgga ggccgggccg gtactgcgcg taggccgcct cgttggtggt
      421 cgtgatgttg tagaacgtca cgatgaggta gagcaggtcc tcgttgccgg ccgggaagtt
      481 ccagcccatc acccggtact cggcgatgat gccgagcggg tgcggccggg cggcgttgag
      541 gcccgggtcg gcctcggacg acatgaacca gacgtcgccc tgcgaggccg acatgcggcc
      601 gcgcagcagc gggtcgaaga gctcctcgga ggcgtcaccc tgcggcacga aggccgcctg
      661 gggccagacc gcctggtcga ccggggacac cgcgttgtac agctcggtga ccgcggtgcc
      721 gtgctggcgg aggccggagg cgtcgaagaa catgccgccg gtggtgtcgc caccccacgg
      781 gtttgccggc ttggtgcccg cgatgatgcc ggccacctgc agaccagagt tgaacatgta
      841 ctggttgccg gtgcccttcg gccagaagcc gccaccgatc gtggacgagt tgagcgagtc
      901 cacgcccact tcgccgcggg tgttcaggcc gtagtagacc cggttgatgg tgatcgcgcc
      961 caggttgccg acgaagagcc ggtagcggcc ggtgttcggc cgcttggcgg ccggatccgg
     1021 gccagcgacg gccggggtcg ggagcgcgac agccgcggcg agcgcgaaca gcagtccgcc
     1081 gatcaggcct cgcgtaccac gcagtgagtg cattgtgcga cctctcgagg atggatgcgg
     1141 gcgccggggc aggggccggc cgaagccgac cccgaccacg gcgagtactc aggtcagaag
     1201 ttcacttcca tgccgaagcg gaggatgcgt ccgccaccgg cccaacggga cacgtggaag
     1261 gtgccgagct gggcgatgtc cgaggcccgc cgctgctctt ccagggtgta gatgccatcg
     1321 ccgttgccgt accgctgctc gcccttgcgg tagaagtagc aggtcggggg cgacgggttg
     1381 ttgcccgtgg tgacccacga gccgcactgc gagtcgctgt ccggcaggtc gatcgcgccg
     1441 gtggtggcgt tgtagatccc gttggccgcc gcgttgagcg cgaaggtcga cgagtcgccg
     1501 gtccaccggg tggctgccag ccgggcattg ctggtggtgc cggtctgcga gaacaccgcg
     1561 aggatgttct tgatgttgaa cacgttgcgg gcgtcggcgt aggcggtcag gtcgagcgcg
     1621 ccgagccgga agcccttggt gacccggagg tcgacgttct tgaacgccgg cagccgcgcc
     1681 gcgttgaagc cgccgcccag gttgttgcag gtgccgcccg aggtgaccgc gaggtcgtcg
     1741 tcgagcgtcg ggtcgcaacg ggtgtacggc gtgcccgagg cgtaccgggc ggtgccgaag
     1801 atgcccatgt tctgcatgat ggcacccacc accgacccct cggagaagtc cgccgggaag
     1861 ttgaacgaga actgggccgc cagcgagtgc gggcgggaga acccgaccgg ctgggccgcc
     1921 tgcggcgggg gcgctgcgct gcccgtgatc gccgaggtga tccgcgaccc gaagttgatg
     1981 taggtgtacg ggtcggtgcc ggtgttgcgg gcgtcctggt acgagtaggc cagcatcacg
     2041 ttgaagatgt tgccgaagcg gcggtcgagc cgcaggtcaa tgccgcgcgt gttgccgaag
     2101 tcggcgttga cggtctgccg caggtccacc cgggagttgg tccgcgggtc gatcgccccg
     2161 atgaggcggc cggccgcgtt ggccaggttg tccttgttgt acacggtcag gtcgagcacc
     2221 atgtcgtcgc tgaacgcatg gcgcacgccg aactcgaaca ggatcgtctt gccgaagtcg
     2281 aggtcggacc cgtagttgtt gttcgtgttg gtgatcgcca ggtcggtgta caccccggta
     2341 ccggtaccat tgacaaactt ac
//
LOCUS       DAOQYE010000267         2356 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_50889_length_2356_cov_2.859192, whole genome shotgun sequence.
ACCESSION   DAOQYE010000267 DAOQYE010000000
VERSION     DAOQYE010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50889_length_2356_cov_2.859192"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="PKN52_05255"
     CDS             complement(<1..299)
                     /locus_tag="PKN52_05255"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HNQ99385.1"
                     /translation="MSVIDIAKGMGLTLGYMFKRPVTVQYPRDKVQIQPRFRGRHHLL
                     RHPDTGLEKCIGCSLCAAACPAYAIYVEAAENDPNDPVSPGERYAEIYEINLLRC"
     gene            complement(342..1361)
                     /locus_tag="PKN52_05260"
     CDS             complement(342..1361)
                     /locus_tag="PKN52_05260"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HNQ99386.1"
                     /translation="MTHDPLWITFVKALIVCVLLLGAFAYMTLIERKLLGRFQHRPGP
                     NMVGPGGWLQPIADAIKTIFKEDLNVTAADKFVLVLAPAISIVFALSAFGAIPAGPAG
                     SLFGFNPWVMDLDIGLLYILAATSLGVYGIFLGGWAANSKYALLGSLRSSAQIISYEL
                     GLGLSVLAVIIVAGTLNLREIVELNIWSVSPWLWPGLLLAFATFVISGTAEVNRTPFD
                     LPEAEQELVAGYLTEYSSIKWALYQMAEYVNMMTASAFISTLFLGGYRGPQFLDAVVP
                     GISEWPIVWLVLKMAIFMFLFIWLKATLPRLRYDQLMRFGWIYVFEFALAAALITGAV
                     VAFVI"
     gene            complement(1358..>2356)
                     /locus_tag="PKN52_05265"
     CDS             complement(1358..>2356)
                     /locus_tag="PKN52_05265"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNQ99387.1"
                     /translation="QAAVGMPTDQARALVARLGAAKQAVVVLGGFALTRREAAEAARA
                     LIKAVGAKELAVGPMANSYGLELLGVLPSHERYGYAGMLDGSKALILSHLDPAQDGEV
                     AERLRGKDLLVVHDIFMTETAHLADVVLPAASVYERDGTVVNLEGRFLPVRAAPVDAG
                     SSADLTGVVRALGEAMGKRLDGRSVRSARRTLKKRFDVDFTELPDHGLLPRAKSRGPA
                     KAVAGEAPSATGNVLVVPSMARAEYLNRNRHLAAALGGNRLRVHPEDAAKHNLAGGAE
                     VRLRVGGTWRQAVVQVTDAVPRGLMTIPSLPEQPIGLAQADLTSIVVEQVRLEVAS"
BASE COUNT          380 a          759 c          872 g          345 t
ORIGIN      
        1 atgcagcgca gcaggttgat ctcatagatc tcggcgtagc gctcgccggg cgagacgggg
       61 tcgttcgggt cgttctcggc cgcctcgacg tagatggcgt acgcggggca ggcggccgcg
      121 cagaggctgc agccgatgca cttctccagg cccgtatcgg ggtgcctgag gagatggtgc
      181 cgcccgcgga agcgcggctg gatctggacc ttgtcgcgcg ggtactggac cgtgacgggg
      241 cgcttgaaca tgtaccccag ggtcagcccc atgcccttgg cgatgtcgat gacgctcatg
      301 ctggagtacc tccgtgacag gcgtgcgagt gggtggcgcg atcagatgac aaacgcgacc
      361 accgccccgg tgatgagcgc cgccgccaaa gcgaactcga agacgtagat ccagccgaag
      421 cgcatcaact ggtcgtaccg caagcgcggc agcgtcgcct tgagccagat gaacaggaac
      481 atgaagatgg ccatcttcag gaccagccac acgatcggcc actccgagat gcccggcacg
      541 accgcgtcga ggaactgcgg cccgcggtag ccgccgagga agagggtgct gatgaaggcg
      601 gacgcggtca tcatgttgac gtactcggcc atctggtaga gcgcccactt gatggaggag
      661 tactccgtca ggtagccggc cacgagctcc tgctcggcct cgggcaagtc gaacggggtg
      721 cggttcacct cggccgtgcc ggagatgacg aacgtggcga aggcgaggag cagcccaggc
      781 cagagccagg ggctcacaga ccagatgttg agctcgacga tctcgcgcag gttgagcgtg
      841 ccggccacga tgatgaccgc gagcacgctc agtccgaggc cgagctcgta cgagatgatc
      901 tgggcgctgg agcgcaggct gccgagcagc gcgtacttgc tgttggcggc ccagccgccg
      961 aggaagatgc cgtacacgcc gagcgacgtc gcggcgagga tgtagaggag cccgatgtcg
     1021 aggtccatga cccatgggtt gaagccgaag aggctgccgg ccgggccggc ggggatggcg
     1081 ccgaaggccg acagcgcgaa gacgatgctg atggccggcg ccagcacgag cacgaacttg
     1141 tcggcggccg tgacgttgag gtcttccttg aagatcgtct tgatggcgtc ggcgatgggc
     1201 tgcagccagc cgccggggcc gaccatgttc gggccggggc ggtgctggaa gcggccgagc
     1261 agcttgcgct cgatgagggt catgtacgcg aaggcgccga ggagcagaac acagacgatg
     1321 agcgccttca cgaaggtgat ccacagcgga tcgtgggtca cgacgccacc tccaggcgca
     1381 cctgctcgac gacgatgctc gtcaggtcgg cttgcgccag gccgatgggc tgctccggga
     1441 ggctcgggat ggtcatcaac ccgcgtggca ccgcgtccgt gacttggacg acggcctgac
     1501 gccacgtgcc accgaccctc agccgcacct ccgcgccgcc ggcgaggttg tgcttggccg
     1561 cgtcctcggg gtgcacgcgc agccggttgc cgccgagcgc ggcggccagg tggcggttgc
     1621 ggttcaagta ctcggccctg gccatgctcg gcacgaccag gacgttgccg gtggcggatg
     1681 gcgcctcgcc ggcgacggcc ttggccggcc ccctgctctt ggcgcgcggg agcagcccgt
     1741 ggtcgggtag ctcggtgaag tcgacgtcga agcgcttctt gagggtccgg cgcgccgagc
     1801 gcacgctgcg cccatcgagc cgcttgccca tggcctcgcc gagggcgcgc acgacgccgg
     1861 tcaggtccgc gctgctgccg gcgtcgaccg gcgcggcccg caccggcagg aagcggccct
     1921 cgaggttgac gacggtgccg tcgcgctcgt agacgctcgc ggccggcagg acgacgtcgg
     1981 ccaggtgggc cgtctcggtc atgaagatgt catggaccac gagcaggtcc ttgccgcgca
     2041 gccgctcggc gacctcgccg tcttgcgcgg ggtcgaggtg cgagaggatg agcgccttgg
     2101 agccgtcgag catgccggcg tagccgtagc gctcgtggct gggcagcacc ccaagaagtt
     2161 ctaggccgta gctgttggcc atcgggccga cggccagctc cttggcgccg accgccttga
     2221 tgagcgcgcg cgccgcctca gccgcctcgc gacgagtgag ggcgaagccg cccagcacga
     2281 ctaccgcctg tttggcggcg ccgaggcggg cgacgagcgc gcgcgcctgg tccgtcggca
     2341 tgccgaccgc ggcctg
//
LOCUS       DAOQYE010000268         2355 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_50929_length_2355_cov_2.442609, whole genome shotgun sequence.
ACCESSION   DAOQYE010000268 DAOQYE010000000
VERSION     DAOQYE010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50929_length_2355_cov_2.442609"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(124..>2355)
                     /locus_tag="PKN52_05270"
     CDS             complement(124..>2355)
                     /locus_tag="PKN52_05270"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNQ99388.1"
                     /translation="GKSSLVFGTIAAESQRMINETYSAFVQGFMPTLARPDVDVLDGL
                     TTAIIVDQERMGANVRSTVGTVTDANAMLRILFSRLGKPHIGSSQAFSFNVPTVRGGG
                     AITVERGPGKAKTESKTYTVLGGMCPRCEGMGTVSDFDLNELYDASKSLNEGALKVPG
                     YSMDGWFGRIFRGCGYFDPDKPIGKYTKQELDDLLYREPTKIKVEGINLTYQGVIPHV
                     QKSFLSKDVEAMQPHIRAFVERVVTFTACPDCGGTRLNETARSSKIKGINIADACAMQ
                     ISDLAEWVKGLDEPSVGPLLTALRDTLDSFVEIGLGYLSLERPSGTLSGGEAQRTKMI
                     RHLGSSLTDVTYVFDEPTAGLHPHDVQRMNDLLKRLRDKGNTVLVVEHKPEVIEVADH
                     VVDLGPGAGSAGGQVMFEGSVKGLRASGTLTGRHLGDRAAIKPAVRKPKGALEVREAK
                     ANNLKDLDVDIPLGVLVVVTGVAGSGKSSLIHGSVSGREGVVAIDQAAIKGSRRSNPA
                     TYTGLLDSVRTAFAKANGVKPALFSANSEGACPVCNGAGVIYTDLGMMAGVTTVCEEC
                     GGRRYQAAVLDYRLGGLNIAEVLDLPASDAVGFFGAGDAKIKAAHDIAGRMVDVGLGY
                     LRLGQPLTTLSGGERQRLKLATHMGEKGGIYVLDEPTSGLHLADVEQLLGMLDRLVDS
                     GKSVIVIEHHQAVMAHADWLIDLGPGAGHEGGEVVFEGTPAALVKAKSTITGEHLAAY
                     VGG"
BASE COUNT          343 a          773 c          833 g          406 t
ORIGIN      
        1 cgcctcgatg gccgcgcaga tacggtggaa ggcgggttcg ccgtccacga gcagcttggc
       61 ggagttgccc tctctaacgg ggtaggtgcc tgagtcgacg aacgggtagg cgccgttcaa
      121 gcgctaccca cccacgtaag cggccagatg ctccccggtg atcgtcgact tggccttaac
      181 gagcgcagcc ggcgtgccct cgaagacgac ctcgccgccc tcgtgaccgg cacccggtcc
      241 gaggtctatg agccagtcgg cgtgcgccat cacggcctgg tgatgctcga tgacgatgac
      301 ggacttgccg gaatcgacga gccggtcgag catgccgagc agctgctcga cgtcggcgag
      361 gtgcagaccg cttgtcggtt cgtcgaggac gtagatgccg cccttctcgc ccatgtgcgt
      421 cgcgagcttg aggcgctggc gctcgccgcc cgacagcgtc gtgagaggct ggccgagccg
      481 caggtagccg aggccgacat ccaccatccg gcccgcgatg tcgtgcgccg ccttgatctt
      541 ggcgtcgccg gcgccgaaga agccgacggc gtcggaggcg gggaggtcca gcacctcggc
      601 gatgttgagg ccgcccaacc ggtagtcgag cacggcggcc tggtagcgcc gcccgccgca
      661 ctcctcgcag acggtcgtga cgccggccat catgccgagg tccgtgtaga tgacgccggc
      721 gccgttgcag acggggcagg cgccttccga gttggcgctg aagagggcgg gcttgacgcc
      781 gttggccttc gcgaacgccg tgcggacgga gtcgagcagg ccggtgtagg tagcggggtt
      841 gctgcgccgc gagcccttga tggccgcctg atcgatggcg acgaccccct ctcgccccga
      901 cacggagccg tggatgagcg agctcttgcc ggagccggcg acgcccgtca ccacgacgag
      961 cacgcccaat gggatgtcga cgtcgaggtc cttgaggttg ttcgccttgg cctcgcgcac
     1021 ctccagtgca cccttgggtt tgcggaccgc cggcttgatg gcggcgcggt cgccgaggtg
     1081 gcgcccggtg agcgtgccgc tcgcgcgcag gcctttcacg ctgccctcga acatgacttg
     1141 gccgccggcg gagccggcgc cggggccgag gtctactacg tggtcggcca cctcgatgac
     1201 ctcgggcttg tgctccacca ccagcaccgt gttgcccttg tcgcgcagcc gcttcagcaa
     1261 gtcgttcatg cgctgcacgt cgtgggggtg gaggccggcc gtcggctcgt cgaagacgta
     1321 ggtgacgtcg gtcagcgagg agcctaggtg tctgatcatc ttcgtgcgct gcgcctcacc
     1381 gccggagagg gtccccgacg ggcgctctag cgataggtag ccgaggccga tctcgacgaa
     1441 ggagtcgagg gtatcccgca acgccgtcag cagcggcccg accgacggct cgtcgaggcc
     1501 cttcacccac tccgccaggt cgctgatctg catggcgcag gcgtcagcga tgttgatgcc
     1561 cttgatctta gacgagcggg cggtctcgtt gaggcgcgtg ccgccgcagt ccgggcaggc
     1621 cgtgaaggtc acgacgcgct cgacgaacgc ccggatgtgg ggctgcatcg cctccacgtc
     1681 cttggagagg aacgacttct ggacgtgcgg gatgacgccc tggtaggtga ggttgatgcc
     1741 ctcgaccttg atcttggtcg gctcccggta gagcaggtcg tccagctcct gcttcgtgta
     1801 cttaccgatg ggcttgtccg gatcgaagta gccgcagccg cggaagatgc ggccgaacca
     1861 gccgtccatg ctgtagccgg gcaccttcag ggcgccctcg ttgagcgact tgctcgcgtc
     1921 gtatagctcg ttcaggtcga agtcgctcac cgtgcccatg ccctcgcagc gcgggcacat
     1981 gccgcccagg acggtgtagg tcttgctctc cgtcttcgcc ttgcccggcc cgcgctctac
     2041 cgtgatggcg ccgccgccgc gaacggtcgg gacgttgaag gagaaggcct gtgacgaccc
     2101 tatgtggggc ttgccgaggc ggctgaagag gatgcgcagc atggcgttgg cgtccgtgac
     2161 cgtgccgacg gtcgagcgga cgttggcccc catgcgttcc tggtcgacga tgatcgcggt
     2221 cgtgaggccg tcgaggacgt ccacgtcggg gcgcgccagc gtcggcatga agccctgcac
     2281 gaaggcgctg tacgtctcgt tgatcatccg ctgcgactcg gcggcgatgg tgccgaagac
     2341 gagcgacgac ttgcc
//
LOCUS       DAOQYE010000269         2352 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_51031_length_2352_cov_2.176317, whole genome shotgun sequence.
ACCESSION   DAOQYE010000269 DAOQYE010000000
VERSION     DAOQYE010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51031_length_2352_cov_2.176317"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..797
                     /locus_tag="PKN52_05275"
     CDS             <1..797
                     /locus_tag="PKN52_05275"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HNQ99389.1"
                     /translation="AQFGAFSPIMQIHGMGKREPWRFEPRTLAIYRAYAQLHTDLFPY
                     LYAAARDASERGVPIMRPVAFAFPGDDAWGDTQEHQYLFGESLLVAPVYVGFNKRKGV
                     WLPRGEDWFELWTGRRFVGGQTVVVDVPLERIPVFARAGSVVPLLADSPRTLDCEGDD
                     PLAAWPDLDVLVYPGKDAAFRLTDGTELRWDDAARRLDVTGSPLRRRVSVHLMDGVGL
                     AMEAHAPGTRNAVGGSPTAVGSVQTDHATSIRLDATNGRSDDAKEE"
     gene            877..1149
                     /locus_tag="PKN52_05280"
     CDS             877..1149
                     /locus_tag="PKN52_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99390.1"
                     /translation="MKKTRLLLVALALMALAVANAQTRVTVWIYESFGGNDSPIAKAA
                     ADFMAQNPDIEIVLVPTASTSAAYRDPFMVAAQGGGGPDVLMADIA"
     gene            1168..2106
                     /locus_tag="PKN52_05285"
     CDS             1168..2106
                     /locus_tag="PKN52_05285"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HNQ99391.1"
                     /translation="MGIVKDLSGHLAGKEDEYFPGPLDTLRYEGGIYGVPFYTNALGM
                     FYNKTAFEEAGLPLPDDDWTWDDFRTAVDTLSNGEMYGFGLEGAWGGTFEWYPWFWQA
                     GGQLLSDDGTTPLFNSEAGLEATRFFLDIVTNPRYVPEAAKTWKGWAELAAAFSNEVI
                     AMYEVGDWGIAMVDGMNPPFEWGVAPLPSNGQRASLVGGANWVMNANTKVEDAAWRWL
                     EYITGPAVFPLMDGYNRVAARRGAAEAQEIVKADPRMQVLTASLEFAKARPTVPLWTT
                     VDYDCLQPAFVRVILEGADVTSEMANAEMCALDVLR"
     gene            2115..>2352
                     /locus_tag="PKN52_05290"
     CDS             2115..>2352
                     /locus_tag="PKN52_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99392.1"
                     /translation="MLKRDERKGLVAPQGATNRRTGRLHRNWPGYVLVLPAMLALVGV
                     IGYPLVRAFVMSLHKLILIRPQLGQPFVGLENYAT"
BASE COUNT          372 a          842 c          786 g          352 t
ORIGIN      
        1 gggcgcagtt cggggcgttc tcgcccatca tgcagatcca cggcatgggc aagcgcgagc
       61 cctggcgctt cgagccgagg accctggcta tctatcgcgc atacgcgcaa ctccacaccg
      121 acctgttccc ctacctttac gccgccgcgc gcgacgcgag cgagcgcggt gttccgatca
      181 tgcgcccggt ggccttcgcg ttccctggtg acgacgcgtg gggcgacacg caggagcacc
      241 agtacctgtt cggtgagtcg ctcctcgtgg cgcccgtcta cgtcggcttc aacaagcgca
      301 agggcgtctg gctgccgcgc ggcgaggact ggttcgagct ctggacgggc cgccgcttcg
      361 tgggcggcca gacggtcgtc gtggacgtgc cgctcgagcg catccccgtg ttcgccaggg
      421 caggctcggt ggtgccactc ctcgccgact cgccgcggac ccttgactgc gagggcgacg
      481 acccgctcgc cgcgtggccc gacctcgacg tgctcgtcta ccctgggaag gacgcggcct
      541 tccgcctcac cgacggcacg gagctgcggt gggacgacgc ggcgcgccgg ctggacgtca
      601 cgggctcgcc cctccgccgc cgcgtgtccg tccacctgat ggacggcgtc ggcctcgcca
      661 tggaggccca cgctcccggg accaggaacg cggtcggcgg gtcgccgacc gccgtaggat
      721 ccgtgcagac cgatcacgcc accagcatcc ggctagacgc gaccaacggc cggtcagacg
      781 acgcgaagga ggagtgaagc agctcaggca ctagagcccc agcccggccc aggccacctg
      841 gtcgccggag acctccaagg acgggagact caacagatga agaagactcg gttactgctc
      901 gtagcgctcg cgctcatggc gttggccgtc gccaacgccc agacgcgcgt caccgtctgg
      961 atctacgaat ccttcggcgg caacgactcg cccatcgcca aggccgcggc ggatttcatg
     1021 gcgcagaacc ccgacatcga gatcgtcctc gtcccgacgg cgtcgacctc ggccgcctac
     1081 cgcgacccgt tcatggtcgc tgctcaaggc ggcggcggac ctgacgttct catggccgac
     1141 atcgcctagt cgcccgagtt cgccgccatg ggcatcgtca aggacctgag cggccacctg
     1201 gccggtaagg aagacgagta cttcccgggc ccgctcgaca ccctgcgcta cgagggcggc
     1261 atctacggcg tgcccttcta cacgaacgcg ctcggcatgt tctacaacaa gaccgccttc
     1321 gaggaggccg gcctgccgct gccagacgac gattggacgt gggacgactt ccggacggcc
     1381 gtcgacacgc tctccaacgg cgagatgtac ggcttcggcc tcgagggcgc ctggggcggc
     1441 acgttcgagt ggtacccctg gttctggcag gccggcgggc agctccttag cgacgacggc
     1501 accacgccgc tgttcaactc cgaggccggc ctcgaggcga cgcgcttctt cctcgacatc
     1561 gtcacgaacc cgcgctacgt gccggaggcg gccaagacgt ggaagggctg ggccgagctg
     1621 gccgccgcct tctccaacga ggtcatcgcc atgtacgagg tcggcgactg gggcatcgcg
     1681 atggtcgacg gcatgaaccc gccgttcgag tggggcgtcg caccgcttcc gtccaacggc
     1741 cagcgtgcca gcctggtagg cggcgccaac tgggtcatga acgccaacac caaggtcgag
     1801 gacgccgcct ggcgctggct cgagtacatc accggcccgg ccgtcttccc gctgatggac
     1861 ggctacaacc gcgtcgccgc ccgccgaggc gccgccgagg cgcaggagat cgtcaaggcc
     1921 gacccgcgca tgcaggtcct caccgcgagc ctcgagttcg ccaaggcccg cccgaccgtc
     1981 ccgctctgga cgaccgtaga ctacgactgc ctgcagccgg cgttcgtgcg cgtcatcctc
     2041 gagggcgcgg acgtgacgag cgagatggcc aacgccgaga tgtgcgccct ggacgtgctg
     2101 aggtagaaga gcgcgtgctg aagcgtgacg agcggaaagg gttggtggcg ccccagggcg
     2161 ccaccaaccg ccgcactggc aggctgcacc gcaattggcc gggttacgtg ctcgtgctgc
     2221 cggccatgct cgccctcgtc ggggtgatcg gctacccgct cgtgcgcgcg ttcgtcatga
     2281 gcctgcacaa gctcatcctg atcaggcccc aactcggcca gccgttcgtg gggctcgaga
     2341 actacgcgac gg
//
LOCUS       DAOQYE010000270         2344 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_51295_length_2344_cov_2.869375, whole genome shotgun sequence.
ACCESSION   DAOQYE010000270 DAOQYE010000000
VERSION     DAOQYE010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51295_length_2344_cov_2.869375"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="PKN52_05295"
     CDS             <1..485
                     /locus_tag="PKN52_05295"
                     /inference="COORDINATES: protein motif:HMM:NF012506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HNQ99393.1"
                     /translation="WRRVYHEQGYAELAFEIQRRYATPDEAQADIGARLEKREVVIGF
                     GHPVYRVSDPRSDIIKAVALELAKEAGDERTYRVAERIETVMHESKRMFPNLDWYSAV
                     AYSVMNVPTAMFTPLFVIARTAGWVAHVIEQREGGTIIRPSANYIGPDDRDFVPIGKR
                     "
     gene            490..1389
                     /gene="prpB"
                     /locus_tag="PKN52_05300"
     CDS             490..1389
                     /gene="prpB"
                     /locus_tag="PKN52_05300"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: protein motif:HMM:TIGR02317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HNQ99394.1"
                     /translation="MPYLVAADLPEERPGARLRALLARPGILPMPGTHNGMAALQARD
                     AGFEALYLSGAAMSASMGLPDLGIMTVDDVSFFIKQVARAGLPVLVDGDTGYGEALNV
                     MQMVRAFEAAGAAAVHIEDQQLPKKCGHLNDKRLVSADDMVLKVAAARKAATDLVVVA
                     RTDAVASEGLSAAVARAERYVAAGAEVIFPEALTTPDMFREFAAALPGVPLLANMTEF
                     GRTPMLSTAEFEELGYKIVIWPVTSLRMANKAQAELYATLRLEGSAKTLLPRMQTRAE
                     LYATIDLEAYEALDAAIVKTVLP"
     gene            complement(1397..2020)
                     /locus_tag="PKN52_05305"
     CDS             complement(1397..2020)
                     /locus_tag="PKN52_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99395.1"
                     /translation="MREEWPGFFERVPRVVVHDPLSDLLGSPVGGIVEYRYQDAVRLA
                     GHSCPAVASAWLLVLRGLAALYGDDLPQRGGVAVGFPTAVTFGTTGVTASVTGLVTGA
                     AAEGGFSGIRGTFGKRRGLMTFGTGLDGHLSMRRLDTGRGVLLGIASEALPPADPEQR
                     QLLDVALTDPQDVQARTRFQQLWVARVERMLTDEAIRANLVTVVEWE"
     gene            complement(2027..>2344)
                     /locus_tag="PKN52_05310"
     CDS             complement(2027..>2344)
                     /locus_tag="PKN52_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99396.1"
                     /translation="NPEVLFVSTFSPTQPEDLYENRVPGQDWSHVKAVQDRRVYRLPE
                     GIFQWYPPSADAPLMMKWIAQQVHPDLFTYDFDAELRDYYQRFYGYTLSDEEIGLILE
                     AAF"
BASE COUNT          388 a          817 c          788 g          351 t
ORIGIN      
        1 gctggcggcg cgtctatcac gagcagggct acgccgaact cgcgttcgag atccagcggc
       61 gctacgcgac ccccgacgag gcgcaggcgg acatcggggc gcgcctcgag aagcgcgagg
      121 tcgtgatcgg cttcggccac cccgtctacc gtgtcagcga cccgcgctcc gatatcatca
      181 aggccgtcgc gctggagctg gccaaggagg ctggcgacga gcgcacgtac cgcgtcgccg
      241 agcgcatcga gacggtcatg cacgagtcca agcgcatgtt cccgaacctc gactggtact
      301 ccgccgtcgc gtactcggtc atgaacgtgc cgactgccat gttcacgccc ctcttcgtca
      361 tagcccgcac ggccggctgg gtggcgcatg tcatcgagca gcgcgagggc ggcacgatca
      421 tcaggccgtc ggcgaactac atcggcccgg acgaccggga cttcgtgccg atcggcaagc
      481 ggtaggcgca tgccgtacct ggtagccgcc gacctgcccg aggagcgccc cggcgcccgc
      541 ctgagagcgc tccttgccag gcccggcatc ctccccatgc cgggcacgca caacggcatg
      601 gccgccctgc aggcgcgcga tgccggtttc gaggcgctct acctctccgg cgcggccatg
      661 agcgcctcca tgggtctacc cgacctcggc ataatgaccg tcgacgacgt ctccttcttc
      721 atcaagcagg tcgcgcgcgc cggcctgccc gtcctcgtcg acggcgacac cggctacggc
      781 gaggcgctca acgtcatgca gatggttcgc gccttcgagg ccgccggggc ggcggccgtg
      841 cacatcgagg accagcagct ccccaagaag tgcggacacc tcaacgacaa gcgcctcgtg
      901 agcgccgacg acatggtcct gaaggtcgcc gccgcgcgca aggccgccac cgacctcgtg
      961 gtcgtggccc gcaccgacgc ggttgcgagc gaggggctca gcgctgcggt cgcgcgggcg
     1021 gagcgctacg tcgccgcggg tgccgaggtc atcttccccg aggccctcac cacgccggac
     1081 atgttcaggg agttcgccgc cgccctgccg ggcgtgccgc tcctggccaa catgacggag
     1141 ttcggccgca cccccatgct gagcacagcg gagttcgagg agctgggcta caagatcgtc
     1201 atctggcccg taacgtcctt acgcatggcc aacaaggcgc aggccgagct gtacgccacg
     1261 ctgaggctag agggcagcgc gaagacgctc ctgccgcgca tgcagacgcg cgcggagctc
     1321 tacgccacca tcgacctcga ggcgtacgag gccctcgacg cggcgatagt gaaaacggtg
     1381 ttgccttagg gtccggtcac tcccactcga ctaccgtcac gaggttggcg cggatcgcct
     1441 cgtcggtgag catgcgctcc acccgcgcga cccaaagctg ctggaagcgg gtgcgggcct
     1501 gcacgtcctg aggatccgtg agtgccacgt cgagcaactg ccgctgctca gggtcggccg
     1561 gcggtagtgc ctccgaggcg ataccgagca gcactccgcg gcccgtgtcg aggcgtcgca
     1621 tggacaagtg tccgtcgagg ccggtgccga aggtcatgag gccccgccgc ttgccgaaag
     1681 tgccgcgtat gccggagaag ccgccctcgg ctgcggcgcc cgtcacgagc ccggtaactg
     1741 aggccgtcac gccggtcgta ccgaatgtta cggcagtggg gaagccaacg gccacgccgc
     1801 cccgttgagg caggtcatcg ccgtacagcg ccgccagacc gcgtagcacg agcagccagg
     1861 cgcttgcgac ggcggggcag gagtgcccgg ccagcctgac cgcgtcctgg tagcgatact
     1921 cgacgatccc gccgaccggg gaaccgagca ggtcgctcaa ggggtcgtga accactaccc
     1981 gcggtacgcg ttcgaagaag ccgggccact cctctcgcat ggtccgctag aaggctgctt
     2041 cgaggatcag gccgatctcc tcgtcgctaa gcgtgtagcc gtagaagcgc tggtagtagt
     2101 cgcgcagctc ggcatcgaag tcataagtga acaggtctgg atggacctgt tgggcgatcc
     2161 acttcatcat cagcggcgcg tccgcgctcg gcggatacca ctggaagatg ccctctggca
     2221 gccggtagac gcgccggtcc tgcacggcct tgacgtggct ccagtcctgc ccgggtacgc
     2281 ggttctcgta gaggtcctcg ggctgcgtcg gcgagaacgt gctgacgaag agcacctcgg
     2341 ggtt
//
LOCUS       DAOQYE010000271         2344 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_51301_length_2344_cov_2.451289, whole genome shotgun sequence.
ACCESSION   DAOQYE010000271 DAOQYE010000000
VERSION     DAOQYE010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51301_length_2344_cov_2.451289"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(36..1568)
                     /gene="nagZ"
                     /locus_tag="PKN52_05315"
     CDS             complement(36..1568)
                     /gene="nagZ"
                     /locus_tag="PKN52_05315"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HNQ99397.1"
                     /translation="MTVLPLMGDLSGTAITADERELLSERRLAGVCLFGRNVADRYQL
                     ADYLAEARSLAGDDLIVAIDQEGGGVLRVLDVPFPPPAMALGAADDVALTEAVAAATG
                     RALAGIGINLDFAPVADVNSNPLNPVIGDRSFGADPALVGRHVAAFVRGLQSSGVGAT
                     LKHFPGHGDTSLDSHLALPTVERSLTDLRHTELPPFQAGFAAGAAAVMSAHIVLPALE
                     PGLPATLSRKALRDLLRDELGYDGLAITDALDMKAISERYSAGAAAVMALRAGADLPL
                     TLGPVTLQRSTLDAIAAAVAAGEVDEGEYAAAVRRIADFARRFPARPATGRPDEAAEA
                     ADYATMAEATRRGLVALGELPRLAPGDRVALIATEAVRANAASQLSARPADGFAAELE
                     RRGVHVQRIHLTWGETADVTPSLGDMNPLPDAVIYASTSRTRVADGEVASAMRAAEWA
                     KYAGVPFAHVALWNPYSAALVSGPALLAFGYRGSAPQAVVDALFSRVVAGKLPVPLAT
                     RA"
     gene            complement(1624..>2344)
                     /locus_tag="PKN52_05320"
     CDS             complement(1624..>2344)
                     /locus_tag="PKN52_05320"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ99398.1"
                     /translation="RHLFGAVGGDYYPLGTDRFGRDLLSRMLVGSQVSLTVGLIGILI
                     SFSIGILVGGVSGYYGGWIDQVLQRLIEVLLSFPRIPILLALATVIPTTWPSTAVYLG
                     IVAVLAIIGWAGLARVVRGQVLAAREMEFVTAAKALGVRDLTVIVKHVVPNLTSYLVV
                     AATLALPGYILGESALSFLGLGVKEPMTSWGLLLKDAQNFESLRLYPWLLAPGALIVL
                     SVLAFNFVGDALRDAADVRSR"
BASE COUNT          362 a          801 c          868 g          313 t
ORIGIN      
        1 ccttctggct tgccgctgac cgggcgtacc ggactctagg ctcgcgtcgc gagcggaacg
       61 ggcagcttcc cggctaccac gcggctaaag agggcatcta ccaccgcctg cggcgcgctc
      121 cctcggtagc cgaaggccaa gagcgcaggc ccggacacca acgcggcgga gtaggggttc
      181 cacaacgcta cgtgcgcgaa cggcaccccg gcgtacttgg cccactcggc ggcgcgcatg
      241 gccgaggcga cctcgccgtc cgcgacgcgc gtgcggctcg tcgaggcgta gatgaccgcg
      301 tctggcagag ggttcatgtc tccgaggctc ggggtgacgt cggccgtctc gccccaggtg
      361 aggtggatgc gttgcacgtg cacgcctcgt cgctccagct cggcggcgaa cccatcggcc
      421 ggtcgcgcgc tgagctggct ggcggcgttg gcacgcaccg cttcggtagc gatgagcgcc
      481 acgcggtcgc ccggcgccag ccggggcagc tcgcccaagg cgactaggcc gcgtctggtg
      541 gcctccgcca tcgtggcgta gtcggccgcc tcggccgcct cgtccggtcg gccagtggcg
      601 gggcgcgccg ggaaacgccg cgcgaagtcc gcgatccgcc gcacggcggc agcgtactcg
      661 ccctcgtcga cttctccggc ggccacggcg gccgctatgg catcgagggt gctgcgctgc
      721 agcgtgacgg gaccgagggt caggggcagg tcggcgccgg cgcgcagggc catgacggcg
      781 gccgcacccg cggagtagcg ctcggagatg gctttcatgt cgagcgcgtc cgttatggcc
      841 aggccgtcgt agccgagctc gtcgcgcagc aggtcgcgca gggccttgcg cgagagggtg
      901 gcgggcaacc ctggctcgag cgcgggcagc acgatgtgcg cgctcatcac cgccgccgcc
      961 cctgccgcga aacccgcctg gaacggcggc agctccgtgt gtcgcaggtc ggtgagcgag
     1021 cgttccacgg tcgggagcgc caagtgcgag tcaagactcg tgtcgccgtg gccagggaag
     1081 tgcttgagcg tcgcgccgac gcctgaggac tggaggccgc gcacgaacgc ggcgacgtgc
     1141 cggccgacga gcgccggatc cgcgccgaac gagcggtctc cgatgaccgg gttgagcggg
     1201 ttcgagttga cgtccgcgac cggagcgaag tcgaggttga tgccgatgcc tgccagcgcc
     1261 ctgccggtgg cggccgccac cgcctccgtc agcgccacgt cgtctgccgc ccccagcgcc
     1321 atggcggggg gcgggaacgg cacgtcgagc acgcgcagca caccgccgcc ttcctgatcg
     1381 atggcgacga tgaggtcgtc gccggccagc gaccgcgcct cggcgaggta gtcggccagc
     1441 tggtagcggt cggcgacgtt gcgaccgaag aggcagacgc ccgcgagcct gcgctcgctc
     1501 agcagctcgc gctcgtccgc cgtgatggcg gtacccgaga ggtcgcccat gaggggcagt
     1561 acggtcatgc tctggtcacc ccgttcgtgg gtagggtcgg tcctcgagtc atatcgaccc
     1621 gggtcacctc gaccggacgt ccgcggcgtc gcgcagggcg tcgccgacga agttgaaggc
     1681 cagcacggag aggactatca acgccccggg cgcgagcagc catgggtaga gcctgagcga
     1741 ctcgaagttc tgcgcgtcct tcaggagtag gccccacgac gtcatcggct ccttcacgcc
     1801 aaggccgagg aacgacagcg cgctctcgcc caggatgtag cccggcagcg cgagcgtggc
     1861 cgcgacgacc aggtaagagg tcaggttcgg cacgacgtgc ttgacgatga cggtgaggtc
     1921 gcgcacgccg agcgccttgg ccgcagttac gaactccatc tcccgcgccg ccagcacctg
     1981 accgcgcacg acgcgcgcca ggcccgccca gccgatgatc gcgagcaccg ccacgatgcc
     2041 caggtagacg gcggtggagg gccaggtcgt ggggatgacg gtggcgagtg cgaggaggat
     2101 ggggatgcgc gggaaactta ggagcacttc gatgaggcgc tgcagcacct gatcgatcca
     2161 gccgccgtag tagccgctaa cgccgcccac caggatgccg atgctgaacg agatgaggat
     2221 gccgatgagg ccgaccgtca gggagacctg cgagcccacc agcatgcgcg agaggaggtc
     2281 gcgcccgaag cgatcggtac cgagcgggta gtagtcgccg cccacggcgc cgaacaggtg
     2341 ccgg
//
LOCUS       DAOQYE010000272         2343 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_51318_length_2343_cov_3.710227, whole genome shotgun sequence.
ACCESSION   DAOQYE010000272 DAOQYE010000000
VERSION     DAOQYE010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2343
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51318_length_2343_cov_3.710227"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..999)
                     /locus_tag="PKN52_05325"
     CDS             complement(<1..999)
                     /locus_tag="PKN52_05325"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HNQ99399.1"
                     /translation="MRFARQLAVLALLTVFCGATAQISETDVKACWIYVGPVGDYGFS
                     YAQDLGRKATAADLPGITTAYVEAVAEADVEATVDQLVADGCNVVFGTSFGFGDGLLA
                     SAERYPNVIFGHATGVERAPNLLTYMADFYQVYYVNGLIAGGLTNSGVVGYIGAFPIP
                     EVKRHIDAFAIGVKEANPDAKVVVRWLYNWFDPAGAKEATEALIADGADVFAFTEDTP
                     TVAQTAAAHGYPSFSHYASMLPYAPDTIASGQLVNWGVLTTDILNKVIDGTYTNTDLD
                     DVDYFWLLAQDAVEVGAEPGMVINPAYEEALKERTVELANGDRLSVYDLALLRIEQM"
     gene            complement(1131..2060)
                     /locus_tag="PKN52_05330"
     CDS             complement(1131..2060)
                     /locus_tag="PKN52_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ99400.1"
                     /translation="MELELLTSTLARALAFGTPLLLAALGEVVAERAGVVNLGVEGMM
                     MLGAVVGFMVAAATGSLPLALLAAVLAGAVVAALHGVIATFLLANQFVSGLAITIGGV
                     GLANLLGKEWVGKPLAAPMLPFDVPLLSAIPVLGPAFFTRQYALTYAALVLAVVIWLV
                     LFRSRPGLTLRTVGESPAAADAAGTNVRRVRFLAVLIGGALAGLAGSYLSLAYRPAWG
                     ENITNGMGWIALGLTILALWHPLRAVPAALLFGAFFTLSFRLQAVLPPELLTLLPYLV
                     TVLALTLIAWRGGRGAVGAPEALGAPYRRGERE"
     gene            complement(2060..>2343)
                     /locus_tag="PKN52_05335"
     CDS             complement(2060..>2343)
                     /locus_tag="PKN52_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99401.1"
                     /translation="FGYTAILVALLARGHAWATLVTAPLLGWVLAAGDVVKVSLQLPF
                     QLTDVMTGLTLLLLVASEPLLTYRPVLVRRPVSPDGQGTGLALRSPEGE"
BASE COUNT          391 a          759 c          888 g          305 t
ORIGIN      
        1 catctgctcg atgcgcagca acgcgaggtc gtagacggag agcctatcgc cgttcgcgag
       61 ctcgaccgtc cgctccttga gggcctcctc gtacgccggg ttgatgacca tgcccggctc
      121 cgcgcccacc tcgacggcgt cttgggctag gagccagaag tagtcgacgt catcgagatc
      181 ggtgttcgtg taggtgccgt cgatgacctt gttcaggatg tcggtcgtga ggacgcccca
      241 gttgacgagc tgcccggagg cgatggtgtc gggcgcgtac ggcaacatcg aggcgtagtg
      301 cgagaagctc gggtagccgt gggcggcggc cgtctgggcg acggtcggcg tgtcttcagt
      361 gaaggcgaag acgtcggcgc cgtcggcgat gagggcctcg gtagcctcct tggccccggc
      421 cgggtcgaac cagttgtaga gccaacgcac cacgaccttc gcgtccgggt tggcctcctt
      481 gacgccgatg gcgaaggcgt cgatgtggcg ctttacctcc gggatcggga acgccccgat
      541 gtagcccacg acgccggagt tcgtgagacc gcccgcgatc aggccgttga cgtagtagac
      601 ctgatagaag tcggccatgt acgtgaggag gttcggcgcg cgttcgacgc cggtggcgtg
      661 cccgaagatc acgttcggat agcgctcggc cgaggccagc aggccgtcgc cgaagccgaa
      721 ggacgtgccg aacacgacgt tgcagccgtc ggcgacgagc tggtcgacgg tggcctcgac
      781 gtccgcctcc gcgactgcct cgacgtacgc tgtggtgata ccgggcaggt cggcggccgt
      841 cgccttgcga ccgaggtctt gggcgtacga gaagccgtag tcgcctaccg gaccgacgta
      901 gatccagcaa gccttgacgt cggtctcgct gatctgcgcc gtggcgccgc agaagacggt
      961 gaggagtgcc aggacggcta gttgacgggc gaaccgcatt cttaagacct ccgaggtcgc
     1021 gccggccggg gctctcgtct cccggtaacg acgcgccgtg actaccaagg tggacgcggc
     1081 gcgtccacct cgcccaccgc cgaccctgcg tcggcggtcg tggccaaggt tcattctcgc
     1141 tccccgcgcc ggtacggcgc gccgagggcc tccggggcgc ccaccgcacc gcgcccaccg
     1201 cgccaggcga tgagcgtgag ggcgagcacc gtgacgaggt acgggaggag cgtcagcagc
     1261 tcgggcggca ggacggcctg cagtcggaag gagagggtga agaaggcgcc gaagaggagg
     1321 gcggccggca cggcccgcag cgggtgccag agcgccagga tcgtgaggcc gagggcgatc
     1381 cagcccatgc cgttggtgat gttctcgccc caggccggcc ggtaggcgag cgagaggtac
     1441 gagcctgcga gcccggccag ggccccgccg atgaggacgg ccaggaagcg cacgcggcgc
     1501 acgttggtcc ccgcggcgtc ggcggcggcc ggcgactccc ccaccgtcct gagcgtgagg
     1561 cctgggcgcg aacggaacag gacgagccag atgacgaccg cgaggacgag cgcggcgtac
     1621 gtgagcgcgt actgccgcgt gaagaacgcc ggtccgagca cggggatggc cgagaggagc
     1681 ggcacgtcga aggggagcat gggcgcggcc aacggcttgc cgacccactc cttgcccagg
     1741 aggttggcga gccccacccc gccgatcgtg atggccaggc cgctcacgaa ctggttggcg
     1801 agcaggaacg tagcgatgac gccgtgcagc gcggcgacga ccgctccggc gagcacggcg
     1861 gcgagcagag cgagcggcag ggagcccgtc gcggcggcga ccatgaagcc cacgacggcg
     1921 ccgagcatca tcatcccctc cacgccaagg ttgacgaccc cggcccgctc ggccacgacc
     1981 tcgccgagcg cggcgagcag gagcggcgtg ccgaaggcga gggcacgcgc caaggtagag
     2041 gtgagcagct cgagctccat cactcgccct ccggggagcg gagcgccagc ccggtcccct
     2101 ggccgtcagg ggagacgggg cgacgcacga gcacggggcg gtacgtgagg agcggctcgg
     2161 aggcgacgag gagcaggagc gtgaggccgg tcatgacgtc ggtgagttgg aagggtagtt
     2221 gcaggctcac cttcacgacg tcccccgcgg cgagcaccca accgaggagc ggggcggtca
     2281 ccagggtggc ccaggcgtgc ccgcgggcca ggagcgccac gaggatggcc gtgtagccga
     2341 agc
//
LOCUS       DAOQYE010000273         2338 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_51472_length_2338_cov_3.642576, whole genome shotgun sequence.
ACCESSION   DAOQYE010000273 DAOQYE010000000
VERSION     DAOQYE010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51472_length_2338_cov_3.642576"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..721
                     /locus_tag="PKN52_05340"
     CDS             <1..721
                     /locus_tag="PKN52_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99402.1"
                     /translation="KLLWAGVLGVLAYLSYQEGQRTGKNLYRLIAIPLAALALVSLFP
                     GATGGALFLAAMGLAFAFVWRRDRSRWWAIIPAGALGTLALIPMLGPAQAHIGSVVFF
                     LGLGATFFALTRLRDHAQPWAVYPAIALAVLALLSISGGASWLVPLFLVAIGLVLLYR
                     TGALGDVERALRGGLGRTSRSDSGTENPTAATGNGAAHENLGGQPPVGQGEVSNPGES
                     LARLGEDSSGRESIPGRDGEG"
     gene            complement(851..1624)
                     /gene="bshB2"
                     /locus_tag="PKN52_05345"
     CDS             complement(851..1624)
                     /gene="bshB2"
                     /locus_tag="PKN52_05345"
                     /inference="COORDINATES: protein motif:HMM:TIGR04000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB2"
                     /protein_id="HNQ99403.1"
                     /translation="MLSEAKGQAADTAPSRTVPFDTVGESAVLAVFPHPDDESFAAGG
                     TLALCNEAGVRTMYLCGTYGDMGRRMGRPAFTNREALRDVRTVELRDACAVLGCELAF
                     MGLRDKCIEFEDPADVAAQVRRVIEEFAPTTVITYYPGHGVHSDHDALGQATVLAVRG
                     MPVAARPRILAVAVGDPAELREQLGEPHVRADIRGVKDRKLDALRAHRSQTETMFAHM
                     DDGGKEDGQTSTFSERLTVREEFYVLDADAPTLVERARG"
     gene            complement(1614..1982)
                     /locus_tag="PKN52_05350"
     CDS             complement(1614..1982)
                     /locus_tag="PKN52_05350"
                     /inference="COORDINATES: protein motif:HMM:NF020409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YojF family protein"
                     /protein_id="HNQ99404.1"
                     /translation="MRPIDRNSIQAELDNRLGQELYLHLETTTGSYTKLGPEKKNPVI
                     AFVRNARISYARGTVTGSGPYRVGLKLDEGWVYADGLTDYDVNERGELLLAGLDCEGQ
                     LTMALQLSATPFREGQTDAE"
BASE COUNT          315 a          823 c          804 g          396 t
ORIGIN      
        1 caagctgcta tgggcgggcg tgctcggcgt actcgcctac ctctcctacc aagaagggca
       61 gcggaccggc aagaacctct accgcctcat cgcgatcccg ctcgccgccc tagcgttggt
      121 gagcctcttc cctggcgcca cgggtggcgc gctcttcctc gccgccatgg gtctggcgtt
      181 cgcgttcgta tggcgccgcg accggagccg ctggtgggcc atcatcccgg ccggcgccct
      241 cggcacgctc gcgctcatac cgatgctcgg ccccgcgcag gcccacatcg gttcggtggt
      301 cttcttcctc gggttggggg cgaccttctt cgccctgacc cgcctgcgtg atcacgcaca
      361 gccatgggcc gtctacccgg cgatcgcact ggccgtgttg gcgctcctgt cgatctctgg
      421 aggggcgagc tggctcgtgc cgctgttcct cgtcgcgatc ggcttggtgc tcttgtaccg
      481 cacgggggcg ctcggcgacg tggagagggc attgcgaggt ggcttggggc gcacgagccg
      541 gagtgactcg ggaacggaga accctaccgc cgccaccggt aatggggccg cacacgagaa
      601 cctaggagga cagcctccgg tcggtcaggg cgaggtctcg aaccccggtg agagcctcgc
      661 gcgcctgggt gaagactcgt cgggccggga gtccatcccc ggtcgcgacg gcgagggttg
      721 acgacgtggc catgaccagg agaggcaaag gcaccctcat cggtggcgga gccatggcgc
      781 tgctcagcgt ggccggttgg cgccggaggt cggcggcgcc ttaggcaacg gggcgcttag
      841 gacccgccgc tcagccgcgc gcccgctcca cgagggtggg cgcgtctgcg tcgagcacgt
      901 agaactcctc gcgaacggtc aggcgctccg agaaggtgct cgtctgcccg tcctccttcc
      961 cgccgtcgtc catgtgggcg aacatcgtct cagtctgcga ccggtgggcg cgcagcgcgt
     1021 cgagcttgcg gtccttcacg ccgcggatgt cggcccgcac gtggggctcg cccagctgct
     1081 cgcgcaactc ggccgggtcg ccgacggcga ccgccaggat gcgcggccgc gccgcgaccg
     1141 gcatgcctcg tacggccagg acggtcgctt ggccgagcgc gtcatgatcc gagtggacgc
     1201 cgtgccctgg gtagtacgtg atcaccgtag ttggcgcgaa ctcctcgatg acgcgccgca
     1261 cctgggcggc gacatcggcg gggtcctcga actcgatgca cttgtcgcgc aagcccatga
     1321 aggcgagctc gcagccaagg acggcgcagg cgtcgcgcag ctccacggtg cgcacgtcac
     1381 gcagcgcctc gcggttcgtg aaggccggcc ggcccatgcg gcggcccatg tcgccgtacg
     1441 tgccgcacag gtacatggtc cgcacgccgg cctcgttgca gagcgccagc gtgccgcccg
     1501 ccgcgaacga ctcgtcgtca gggtgcggga acacggcgag cacggcgctc tcgccgacgg
     1561 tgtcgaacgg cacggtgcgg ctgggggccg tgtcggccgc ttgacctttg gcttcactca
     1621 gcatcggtct gtccttctct gaagggcgtg gcggagagtt gcaacgccat cgtgagctgc
     1681 ccctcgcagt cgaggccggc gaggaggagc tcgccgcgct cgttgacgtc gtagtcggtc
     1741 agcccgtccg cgtacaccca tccttcgtcg agcttcaggc cgacgcggta ggggccgctg
     1801 cccgtcacgg tgccgcgcgc gtaagagatg cgcgcgttgc gcacgaacgc gatcactggg
     1861 ttcttcttct ccggcccgag cttcgtgtac gagcccgtgg tcgtctcgag gtgcaagtac
     1921 agctcttgcc cgagcctgtt gtccagctcg gcttggatgg agttgcggtc gatgggcctc
     1981 acctgaacct ccgagatacc ttggcgccag ccgccgcccc aaaagctcgt ggcgcttcgt
     2041 gcccgcggga acgccatccc gaggcgatag ggcggattct accgccgctc acgttacggc
     2101 gctcttacgc tcgcttcatc taccttccga acaccgcccc gcctctccgg tcccgcaccg
     2161 taccgtggtc cgacaggctg gccgtcgacc ccggagagcg ccgggcgctg gctatgattc
     2221 gcgcatgaac tggttacccg ccgtgcagga cgaactggcc aggcaaggca tcgacgcttg
     2281 gctcgtgtac gacttccgcc gctccaaccc ggtggcggcc cccgtgctcg gcccgctg
//
LOCUS       DAOQYE010000274         2335 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_51590_length_2335_cov_2.644298, whole genome shotgun sequence.
ACCESSION   DAOQYE010000274 DAOQYE010000000
VERSION     DAOQYE010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51590_length_2335_cov_2.644298"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(337..783)
                     /locus_tag="PKN52_05355"
     CDS             complement(337..783)
                     /locus_tag="PKN52_05355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99405.1"
                     /translation="MKPTLPDVNVLIALLDPLHVHHERAHAWFAEARREEWLSSPTTQ
                     NGVLRIVSHPKYPNHQPLQTVLESLRSLLAVGKHRFVPDDHSLAWAAKGLKSVITPER
                     LAISAQVTDTYLLALAVANDAQLATFDRRMFTGAVKGGDKALLLIP"
     gene            complement(780..1016)
                     /locus_tag="PKN52_05360"
     CDS             complement(780..1016)
                     /locus_tag="PKN52_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99406.1"
                     /translation="MMQHRTTLSIDEDVLAYARQLSEVSGKPLGTIISELARAAMTKT
                     GAAPVRNGIQLLPIGKGATSATLEDVNRLRDESP"
     gene            1139..>2335
                     /locus_tag="PKN52_05365"
     CDS             1139..>2335
                     /locus_tag="PKN52_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99407.1"
                     /translation="MQRTADIGAVGDGYYDMGAYSRRVRTPSPEAQLWFDRGLAWCYG
                     YHHEEAIRCFQRAATADPGCVMAHWGVAYAAGCNYNKPWEAFLDEERRAALALAHDAA
                     REAAELVRAAGVDAWPSEAALAGALLRRYPVAEALADAPFQAWNDDYASAMRDVYAAH
                     PDDPDVAALFAEALINRTPWRLWNMATGEPAAGADTLEARAVLERAIAAREAVGSSGH
                     PGLLHIYVHTMEMSPTPEAALRAADALRDLVPGSGHLRHMPSHIDVLCGNYHDSLVAN
                     ELAMEADDRLFASGGGGDFYTLYRCHNIHFKIYSALFLGQSGPALEGARQLEEVLPES
                     LLRVELPPMADWLEGFLSMRVHALIRFGRWHDVLALPLPSDPDLYCVTTAMTRYARGV
                     ASAALGD"
BASE COUNT          358 a          754 c          807 g          416 t
ORIGIN      
        1 gatacgagcc gggatgatcc tctccgtcga gccgggcatc tacgttcccg ggctggccgg
       61 gttccggcat tccgatacgg tccttatcac cgaggaaggg tgcgagaggt tgtcgctgta
      121 cccgcggggg ttgagcgaga tggtgatcga gcgttagggc ggagggccct gttggcgcgc
      181 cgagatctat gaggggctcc tggtaaagcc gcgttggcac ttctcgtcag tcccgcagga
      241 ggaggctctc ctcggcatcc catgttgagg gagtgaatgg ttgggctcaa tgggaagcga
      301 gggctcgggt ctccggttgt cgtatcgacc agctcgtcac gggatcagca gcaaggcctt
      361 gtctccgccc ttaacggcgc ccgtgaacat gcgcctatca aaggtggcga gttgagcgtc
      421 gttcgctacc gccagcgcca gcagataggt gtcggttact tgcgccgaaa tggcgaggcg
      481 ctccggagtg atcacactct tgagcccctt ggcggcccat gccaggctgt ggtcgtccgg
      541 tacgaagcgg tgcttgccta cggcgaggag gcttcgcaag ctctctagca ccgtttgaag
      601 cggctggtgg ttcgggtact ttgggtgcga cacgatccgg agcacgccgt tctgggtagt
      661 gggtgaggac agccactcct cgcgccgcgc ctcggcgaac cacgcgtgtg cccgctcgtg
      721 gtgtacgtgg agcgggtcga gcaaggcgat caggacgttg acatccggca gcgtgggctt
      781 cacggcgact cgtcccgcag gcggttgacg tcttccaacg tcgcgctcgt cgcgcccttg
      841 ccgataggca gcagttggat gccgttgcgg accggtgctg ctcccgtctt cgtcatggcg
      901 gcgcgagcga gttcggagat gatggtgcca agcggtttac cgctcacctc cgatagctgc
      961 ctcgcgtatg cgaggacgtc ttcgtcgatc gacaacgtcg tgcgatgctg catcatgcac
     1021 caagcatcaa gcatcatcaa gttcgcgtca accacacccg gtaacatgcc caagcaacac
     1081 cactaggcag caccgccgcg gaccctgtcc acgcggtcgc ctgacgtagg aggcgaacgt
     1141 gcagcgaacg gctgatatcg gcgcggtcgg cgacggttac tacgacatgg gcgcgtactc
     1201 gaggcgcgtg cggacgccgt ctcccgaggc gcagctctgg ttcgaccgcg ggctcgcctg
     1261 gtgctacggc taccaccacg aggaggccat ccgctgcttc cagcgcgccg ccaccgccga
     1321 ccctggctgc gtcatggcgc attggggcgt cgcgtacgcc gccggctgca actacaacaa
     1381 gccgtgggaa gcgttcctcg acgaggagcg ccgcgccgcc ttggctctag ctcacgacgc
     1441 tgcccgcgag gctgccgaac tcgtgagggc ggccggggtg gacgcctggc cttccgaggc
     1501 ggcgcttgca ggggcgctgc tacgcaggta cccggtcgcc gaagccttgg ccgacgcgcc
     1561 gttccaggcg tggaacgacg actacgcgtc cgccatgcgg gacgtgtacg ccgcccatcc
     1621 ggacgatccg gacgtggccg cgctgttcgc cgaagcgctg atcaaccgca ccccttggcg
     1681 gctctggaac atggcgacgg gcgaaccggc cgccggcgcc gacacgttgg aagcccgtgc
     1741 tgtcttggag cgggcgatcg cggcgcggga ggcggtgggc tcgagcgggc atccgggcct
     1801 gctgcacatc tacgtccaca cgatggagat gtcgccgact ccggaggcgg cgctgcgtgc
     1861 ggccgacgcg ctgcgtgacc tcgtgcccgg ctccgggcac ctgcgtcata tgccgtcgca
     1921 catcgacgtc ctgtgcggca actaccacga ctcgctcgtc gccaacgagc tggccatgga
     1981 ggcggacgac cggctgttcg caagtggtgg tggcggtgat ttctacacgc tctaccgctg
     2041 ccacaacatc cacttcaaga tctactcggc gctcttcctg gggcagtcgg gtccggcgct
     2101 cgagggggcc aggcagctgg aggaggtgct gccggagagc ctcctgcgag tcgagctgcc
     2161 acccatggcc gactggctcg agggtttcct gagcatgcgc gtgcacgcgc tgatacgctt
     2221 cgggcgttgg cacgacgtgc tggccctccc gctgccgtcc gatcctgatc tctactgcgt
     2281 cacgacggcc atgacacgct acgcgcgtgg cgtggcgtcg gcggcgctcg gcgac
//
LOCUS       DAOQYE010000275         2333 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_51661_length_2333_cov_2.693152, whole genome shotgun sequence.
ACCESSION   DAOQYE010000275 DAOQYE010000000
VERSION     DAOQYE010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51661_length_2333_cov_2.693152"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..699)
                     /locus_tag="PKN52_05370"
     CDS             complement(<1..699)
                     /locus_tag="PKN52_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HNQ99408.1"
                     /translation="MTLADIMLYEGKASEFVLQDDLGDDGAARSHLLTTMLGRIGEMR
                     DSIRRGLASTAPSRTGMVGWNAKTLHDAPDALDAPLLKRVQAYAMAVNEENARMGRIV
                     AAPTAGSAGTVPGALIGVADHLGKDDSDLVMPLVLAAGIGQVISKSMFIAGSTGGCQA
                     EIGSSAAMAAAAVTELLGGTARACVHAAAQALMNTIGLVCDPVGGYVEVPCVSRNAFY
                     AVHAVSAAQLALAGV"
     gene            complement(696..1484)
                     /gene="sdaAB"
                     /locus_tag="PKN52_05375"
     CDS             complement(696..1484)
                     /gene="sdaAB"
                     /locus_tag="PKN52_05375"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HNQ99409.1"
                     /translation="MSLLDQIGPVMVGPSSSHTAGACRLALLARNVLGAEPRRATFVL
                     HGSFAKTAKGHGTDLALVAGTLGYFPDDERIRDAFEHAATAGLEYEFTTADLGDIHPN
                     SVLMELSAGGDGANVSVLGSSLGAGFVRISQVNGFDVQVSGAYHALLALHTDRPGVIA
                     RVASVVADDQGNIATAFSARQRRGGHAMLSMEIDRPLADYAVTYLSSLPYIHWLRQLP
                     AVMAGDAPVTAGAPAVAGAPATIGAPAETEASALASADADGDAR"
     gene            complement(1596..2249)
                     /locus_tag="PKN52_05380"
     CDS             complement(1596..2249)
                     /locus_tag="PKN52_05380"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNQ99410.1"
                     /translation="MAERPKQRRGFLEWLLSPQWFMPTAIWVVRTYARLFRGLRITGL
                     ENVPQGVGLVVACNHESSWDPPIVGVSINRKLEFMAKKELFAKPFSRAIMRGLRAFPV
                     DREGQDIGAIKEALRRLKEGRAIGVFVQGTRNAGDAAALDGAAYLAQRAGTQLLPAAI
                     WRENGKFRVAFGPPLAAAGRTRDDASELTRAVMLEIAALLPAQGERDGVSAAGKTPR"
BASE COUNT          325 a          808 c          851 g          349 t
ORIGIN      
        1 gacgccggcc agggccagct gcgccgccga taccgcgtgc acggcataga aggcgttgcg
       61 ggagacgcac ggtacctcga cgtaaccgcc cactgggtcg cacaccaagc cgatggtgtt
      121 catcagggcc tgggcggcgg cgtgcacgca cgcgcgggcc gtgccgccga gcaactcggt
      181 gacggccgcg gccgccatgg cggcgctcga gccgatctcg gcctggcagc cgccggtcga
      241 gccggcgatg aacatgctct tgctgatgac ttgcccgatg cccgcagcca ggacgagggg
      301 catcacgagg tcggagtcgt ctttgcccag gtgatcggcc acgccgatga gcgcgcccgg
      361 cacggtcccg gcgctgccgg ccgtcggcgc ggcgacgatg cggcccatgc gcgcgttctc
      421 ctcgttcacg gccatggcgt acgcctgcac gcgtttgagg agcggcgcgt ccagggcgtc
      481 cggggcgtcg tggagggtct tggcgttcca cccgaccatg ccagtgcgac tgggggccgt
      541 gctggcgagg ccgcggcgga tggagtcgcg catctccccg atccgcccga gcatggtggt
      601 gaggaggtgg ctcctggcgg ccccgtcgtc gccgagatcg tcttgcagga cgaactcgga
      661 cgccttgccc tcgtacagca tgatgtcagc tagcgtcatc gcgcgtctcc atctgcgtcc
      721 gcgctcgcaa gcgcggacgc ctccgtctcg gcgggcgcac ctatcgtcgc aggagcgcct
      781 gcgacagcag gcgctcctgc cgtcacaggc gcgtcgcctg ccatgaccgc cggcagttgc
      841 cgtagccaat ggatgtacgg caggctgctc aggtaggtga ccgcgtaatc ggcgagcggt
      901 cggtcgatct ccatggacag catggcgtgc ccaccgcgcc gctgacgggc cgagaacgcc
      961 gtggcgatgt tgccctgatc gtcggcgacg acgctcgcga cgcgggcgat gacgcccggc
     1021 ctgtccgtgt gcagcgccag cagcgcatgg taggcgccgc tcacctggac gtcgaagccg
     1081 ttgacctggc tgatgcgcac gaaccccgcc ccgaggctcg agccgagtac ggaaacgttg
     1141 gcgccgtcgc cgccggcgct caactccatg agcacgctgt tcgggtggat gtcgcccagg
     1201 tcggcggtcg tgaactcgta ctcaaggccg gccgtcgccg cgtgctcgaa agcgtcgcgg
     1261 atgcgctcgt cgtcggggaa gtagccgagc gtgcctgcta ccagcgccag gtcggtgccg
     1321 tggcccttcg cggtcttggc gaacgagccg tgcagtacga aagtggcgcg ccgcggttcg
     1381 gcgcccagca cgttccgtgc gaggagcgcc aagcggcagg cgcccgccgt atgcgagctg
     1441 gaaggcccga ccatgacggg gccgatctgg tcgaggagcg acatgcgacc tcaagtgtac
     1501 tcgcgtcgcc gcgccgcgcc ggtgcggcgc gagcgctgtg agaaacacga cgaacgcgcc
     1561 ggggcgcctg cgttactcgg ctcgagcctg tccggctacc ggggggtctt gcccgcggcg
     1621 ctcacgccgt cccgctcgcc ctgcgcgggc agcagcgcgg cgatctccag catcacggcc
     1681 cgcgtgagct cggaggcgtc gtccctcgtg cggcccgcgg ctgcgagcgg cggtccgaac
     1741 gcgacccgga acttgccgtt ctcgcgccag atggcggcgg gcaggagctg ggtgccggcg
     1801 cgctgagcga ggtaggcggc cccgtccaac gcggcggcgt cgccggcgtt gcgtgtgccc
     1861 tgtacgaaga cgccgatggc cctaccctcc tttaggcgcc tcagcgcctc cttgatggcc
     1921 ccgatgtcct gcccctcccg gtcgaccggg aacgcgcgca ggcctcgcat gatcgcgcgg
     1981 ctgaacggct tggcgaacag ctccttcttc gccatgaact cgagcttccg gttgatcgag
     2041 acgccgacga tgggcggatc ccagctgctc tcgtggttgc aggcgacgac gagcccgacg
     2101 ccctggggaa cgttctcaag ccccgtgatc cgcaggccgc ggaagagccg ggcgtacgtg
     2161 cgtacgaccc agatagcggt cggcatgaac cactgtggcg agaggagcca ctcgaggaag
     2221 ccgcggcgtt gtttggggcg ttccgccatg cggaggctca gcggacgcgc tcggccggct
     2281 ggcgccgacc gccctgaggg tcgcggcggg ggtcggcgat acgcgacgcg tca
//
LOCUS       DAOQYE010000276         2331 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_51735_length_2331_cov_2.385764, whole genome shotgun sequence.
ACCESSION   DAOQYE010000276 DAOQYE010000000
VERSION     DAOQYE010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51735_length_2331_cov_2.385764"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            183..650
                     /locus_tag="PKN52_05385"
     CDS             183..650
                     /locus_tag="PKN52_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99411.1"
                     /translation="MSLLQRLIGAAMGPRVKRRARGKTFGQLADQLAASRDYLLPKVM
                     AAKDTPGNREALNHWVGIERWALDNARSWEHPHDPRPYHPFRLPSGASLAELQQAFAS
                     AREESIVAAREFDRTGVDPAAIVVHKDFGPLTVTEWLEYIDDHSRREVIRLRR"
     gene            complement(690..1583)
                     /locus_tag="PKN52_05390"
     CDS             complement(690..1583)
                     /locus_tag="PKN52_05390"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HNQ99412.1"
                     /translation="MSRPFGLAAVQMAVEPWDPPATATKMEDVARRIVNSFPWVDMLV
                     FPELAGSGVAQFGRVPSRDEYARCRAPIPGHLTEELCAVARRLGRWLVPGSVYELDGD
                     DCYNTAIAISPEGEIVARYRKIFPWYPFEAEAKAGGDYGVFDVPGVGRFGLSICYDMW
                     FPETVRTLAWMGAEVILHPTLTTTQDRELETVLSRAHAITNQVYFLDVNAVGAWGGGR
                     SLLVGPEGLVLHQAGERETFFVQRIDLDDVARARETGTLGIAQTWKQLRDYGMRFPPY
                     QEGFERGEVFKGLGELKLRGR"
     gene            complement(1609..>2331)
                     /locus_tag="PKN52_05395"
     CDS             complement(1609..>2331)
                     /locus_tag="PKN52_05395"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ99413.1"
                     /translation="MGEGRSSVSTRISAPSKEFVLGTDRLGRDVLSRIIYGVRPALIA
                     PLIVVLLAVAIGAPLGAIAGLAGGWVDEAIMRVCDLFLAFPSLLLAMAIVALQGPSLT
                     NAVIALAVSWWPWYTRLVRGVALSLRHQAFVEAARALGASTPKIMLRHILPNSVTPIL
                     VQASLDVGTVILATTGLAFIGLGSQPPAADWGLMIEDGRALLRTAWWTSTFPGLAIFL
                     SVLSFNLVGDALRDLFDPKEYR"
BASE COUNT          382 a          791 c          835 g          323 t
ORIGIN      
        1 gccgcgcggg tcgcggtcga tgacgaagct caccgccgcg ttgcagaggt tgcacacgcc
       61 gtcgaaaagg aggatcgggc cgccgtcgtc cggtgccggc atcacggctc cagtctccca
      121 tgccaccgcc tgtcgcgcgg tgcgcccagg cgcaccccgg tcgccagtac actcccaggc
      181 gtatgagcct tctgcagcgg ctgatcgggg cggccatggg gccgcgcgtg aagaggagag
      241 cgcgaggcaa gacgttcggg cagttggccg accagctggc cgcttcgcgc gactacctgc
      301 tgccgaaggt gatggcggcc aaggacacgc ccggcaaccg ggaggcgctc aaccattggg
      361 tcggcatcga gcgctgggcc ctcgacaacg cccgaagctg ggagcacccg cacgacccgc
      421 ggccatacca cccgttcagg ttgccgtcgg gagcctcgct cgccgagctg cagcaggcgt
      481 tcgcgtcagc gcgcgaggag agcatcgtcg cggcgaggga gttcgaccgg acgggcgtcg
      541 accccgccgc catcgtcgtc cacaaggact tcgggccgct cactgtcacc gagtggctcg
      601 agtacataga cgatcactcg cggcgcgagg tcatcaggct gcgtcggtag cggtgcggcg
      661 gccggcggcg gagaggcggt ccggtgcgtt cagcgccccc gcaacttcag ctcgccgagc
      721 cccttgaaca cctcgccgcg ctcgaaccct tcctggtagg ggggaaagcg catgccgtag
      781 tcacgcagct gcttccacgt ctgggcgatg ccgagggtgc ccgtctcgcg ggcgcgcgcc
      841 acgtcgtcga ggtcgatgcg ttgcacgaag aacgtctcgc gctcgcccgc ctggtgtagg
      901 acgagacctt ccggtccgac gagcagcgag cgcccgccgc cccacgcgcc cacggcgttc
      961 acatcaagga agtacacctg gttcgtgatg gcgtgcgcgc ggctcaggac ggtctccagc
     1021 tcccggtcct gtgtggtggt gagggttggg tggaggatca cctccgcgcc catccatgcg
     1081 agcgtcctca ccgtctcggg gaaccacatg tcgtagcaga tggagagccc gaagcgcccg
     1141 acgcccggca cgtcgaacac cccgtagtcg ccgccggcct tggcctcggc ctcgaacggg
     1201 taccagggga agatcttgcg gtagcgcgcc acgatctcgc cctcgggcga gatggcgatc
     1261 gccgtgttgt aacagtcgtc gccgtccagc tcgtacaccg agcccggcac gagccagcgg
     1321 ccgaggcgcc tcgccactgc gcacagctcc tcggtcagat ggccagggat gggagcgcgg
     1381 caacgtgcgt actcgtcgcg gctcggcacg cgtccgaact gggccacgcc ggaaccggcc
     1441 aactcgggga acaccagcat gtccacccac gggaaggagt tgacgatgcg ccttgcgacg
     1501 tcctccatct tggtcgcggt cgccggcggg tcccatggct cgacagccat ctgcacggcg
     1561 gccagtccga aaggccggct cacgaggtcg cctcgttcag gtgaggtctc atctgtactc
     1621 cttggggtcg aagaggtcgc gcagcgcgtc tccgacgagg ttgaagctca gcacggacag
     1681 gaagatggcg aggccgggga aggtgctcgt ccaccaggcc gtgcgcaata aggcgcgacc
     1741 gtcctcgatc atcaggcccc agtccgccgc cggcggttgg ctgccgagcc cgatgaaggc
     1801 gaggccggtt gtggcgagga tcaccgtgcc gacgtcgagg gaggcctgca cgaggatcgg
     1861 ggtgacggag ttggggagga tatgccgcaa catgatcttc ggcgtgctcg cgccgagcgc
     1921 cctggccgcc tcgacgaacg cctggtggcg cagcgagagc gccaccccgc gcacgagacg
     1981 cgtgtaccac ggccaccagg agacggccag cgcgatgacg gcgttggtga ggctcgggcc
     2041 ttggagcgcg acgatggcca tggccaggag gagggagggg aaggcgagga agaggtcgca
     2101 gacgcgcatg atcgcctcgt cgacccagcc tcccgccagg ccggcgatgg cgccgagcgg
     2161 cgccccgatg gccacggcca ggagcacgac gatgagcgga gcgatcaggg cgggccgcac
     2221 gccgtagatg atgcggctca gcacgtcgcg cccgagccgg tccgtgccga gcacgaactc
     2281 cttggacggt gccgagatgc gggtcgagac ggacgagcgc ccctcgccca t
//
LOCUS       DAOQYE010000277         2330 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_51773_length_2330_cov_1.955604, whole genome shotgun sequence.
ACCESSION   DAOQYE010000277 DAOQYE010000000
VERSION     DAOQYE010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51773_length_2330_cov_1.955604"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /locus_tag="PKN52_05400"
     CDS             complement(<1..619)
                     /locus_tag="PKN52_05400"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNQ99414.1"
                     /translation="MPAAVLEAVARPVPHHRTTAARETFLSARTRLAEVFCVPGDDVL
                     IVTGSGTAGFEAALVSSVPAGGTVLALHAGKFGERWAGMAERLGYAVERFGSGAGVEF
                     DTQELTARLRALPELAAVTLVHSETSTGALHDVRAVAALVRAERPEALVLVDAVTSLA
                     SAELRPRDWDLDAVVSGSQKGVMTPPGLAFVWLSERAWRRGAKSSQ"
     gene            complement(751..1434)
                     /locus_tag="PKN52_05405"
     CDS             complement(751..1434)
                     /locus_tag="PKN52_05405"
                     /inference="COORDINATES: protein motif:HMM:NF039288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HNQ99415.1"
                     /translation="MAVLKGDYSVGIVNAPALAPNVMAALGVHLTLGERLGGMATQLA
                     RGRVKAVEVEFSGDFPMDPDPVATAVVKGLMEPFLDTPPTYVNAPSLARDRDIRVGKV
                     TSSANHGYTTHVLVTVEAADGMTRVGGTVLAGQPRITSIDGYNLEIRPEGAMLVCTNV
                     DRPGAVGRIGTVLGDAGVNINSMQLSRVSDHGLAMFVVSLDSAPSEEVLEVLAGMTDV
                     IRSLRLVRL"
     gene            complement(1547..2005)
                     /locus_tag="PKN52_05410"
                     /pseudo
     CDS             complement(1547..2005)
                     /locus_tag="PKN52_05410"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
BASE COUNT          316 a          844 c          855 g          315 t
ORIGIN      
        1 gctggctgct cttcgcgccg cgccgccacg cccgctccga tagccacacg aacgcgagcc
       61 ccggcggcgt catcacgccc ttctgggaac cggagacgac ggcgtcgagg tcccagtcgc
      121 gcggccgcag ctcggcgctg gcgaggctcg tgaccgcgtc gacgagcacg agcgcctcag
      181 ggcgctcagc gcgcacgagc gcggccacgg cgcgcacgtc gtggagcgcg cccgtcgagg
      241 tctcgctatg cacgagggtc acggcagcca gctcaggcag cgcgcgcagc cgcgccgtca
      301 actcctgcgt gtcgaactcg acgcccgcgc cggacccgaa ccgctccacc gcgtagccga
      361 ggcgctccgc catgccggcc caccgctcgc cgaacttgcc ggcatggagg gccagcacgg
      421 tgcccccggc gggaacgctg ctgacgagtg ccgcctcgaa gcctgcggtg ccgctaccgg
      481 tgacgatgag cacgtcgtcg ccaggcacgc agaagacctc ggcgaggcgg gtccgggcgc
      541 tgaggaacgt ctcgcgcgcc gcggtcgtcc ggtggtgcgg cacggggcgg gcgacggcct
      601 cgaggaccgc cgccggcacc tccaccggcc cgggggcgag tagccgctcc ctgaagcccg
      661 cgtgtccgcc ggcgcccgag ggccggaccg gggccggcga ccgctccggt ccgaccggcg
      721 ccgccgcggc ggtcggcggt tcgcgtccgg tcacaagcgc accagccgca ggctacggat
      781 gacgtcggtc atgcccgcga ggacctcgag cacttcctcg ctcggggccg agtcgagact
      841 gaccacgaac atggcgaggc cgtggtcgct cacccgggag agctgcatgc tgttgatgtt
      901 cacgcccgcg tcgccgagca cggtgccgat gcgcccgacg gcccccggcc ggtcgacgtt
      961 cgtgcagacg agcatggcgc cctcggggcg gatctccagg ttgtagccgt cgatgctcgt
     1021 gatgcgcggt tggccggcca gcacggtgcc acccacccgc gtcatgccgt ccgccgcctc
     1081 taccgttacg agcacgtgcg tggtgtagcc gtggttggcg ctcgaggtca ccttgccgac
     1141 gcggatgtcg cggtcgcggg ccagcgacgg cgcgttcacg taggtgggcg gcgtgtcgag
     1201 gaacggctcc atgagcccct tgaccaccgc cgtggcgacg gggtcggggt ccatcgggaa
     1261 gtcgcccgag aactcgacct cgacagcctt gacgcgcccg cgggcgagct gcgtggccat
     1321 gccgcccaac cgttcgccga gcgtgaggtg gacgccgagc gccgccatca cgttcggggc
     1381 caaggccggg gcgttcacga tgccgacgga gtagtcgccc ttcaggacgg ccaacgtgcg
     1441 ctaggatctg ctcgcccacc cggtgctgcg cctcgcgggt gttggcgccc aagtgcgccg
     1501 tcaggacgac gtcgtcgcgt tgcaggagcg ggtgatcggg tcgcggcggc tcgagcacgt
     1561 agacgtcgag ccccgccccg aagagccggc cggactccag cgccgccagg agcgcgtcct
     1621 cctggatgat gccgccgcga gccgcgttca cgaccaccgc gccgaccggc agggcggcca
     1681 gctcgtccag cccgagcatg ccgcgggtct cctccgtgag gggcgtgtgg acggtcaggt
     1741 agtccgagcg cgccagcatg gcggggaggt cgtcgaacag ctcgaccttc agctcgtcgg
     1801 cgcggcgccg gttgatgtac gggtcgtacg ccgccacttg catccccagg ccttgggcct
     1861 tgcgcgccac cagggagccg atgcggccga ggccgacgat gccgaggcgc gcggactcga
     1921 cctcgcgtcc aagatacgcg cggtcccact taccgctccg gaggagcttg tcggagcggg
     1981 ccacgccgcg ggcggcggcg agcatcaacg cgatggccag ttcggccgcg gagaaccccg
     2041 cctgcttcat caggtccagc agcggcaccg tctcgccggc cgccacggcc ccctcggatc
     2101 cggcggcgcg gaagtcccag tagatgcgtt cgtagtgggc ggggcgcggc ttcttgccgt
     2161 tgcggatgtc gaggatctcg aagtattgct gctcccactt gggctctccg ccgctcacga
     2221 cgaaggtgcg ggccagccgc gtgaggtcgt cggaactctg gcgcaattca tcggccagca
     2281 ggaacgattg gtagcgacgg gcgccggcct cgcgggcggc gtcggcgtgg
//
LOCUS       DAOQYE010000278         2329 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_51793_length_2329_cov_3.417766, whole genome shotgun sequence.
ACCESSION   DAOQYE010000278 DAOQYE010000000
VERSION     DAOQYE010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51793_length_2329_cov_3.417766"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..778)
                     /locus_tag="PKN52_05415"
     CDS             complement(<1..778)
                     /locus_tag="PKN52_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNQ99416.1"
                     /translation="MPGSDTGSDTGSDNSPAWVQDAVFYQIFPDRFARSSRVHKPSNL
                     EEWDAPPTLHGYKGGDLLGVVEKLDYLQDLGVTALYLNPIFASGSNHRYHTQDFHQVD
                     PMLGGNAALDELIAACHKRGMRVVLDGVFNHASRGLLQFHDILENGPSSAYLDWFHVR
                     HFPLHAYGGGQLGYDAWWGLAALPKFNTDTPAVREFIYQVAEKWLAAGMDGWRLDVPN
                     EIDDDDFWRAFRTRCRAVNPDAYLVGEIWDEAGRWLKGDIF"
     gene            825..1535
                     /locus_tag="PKN52_05420"
     CDS             825..1535
                     /locus_tag="PKN52_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99417.1"
                     /translation="MILPRRPLSWRVFLLRLLQLNVGLVIYGLSVALMVNAGIGLGPW
                     DVFHQGLSRLTPLTIGQAMILAGLAILVYSVIGPKVRIGIATVLNMVLIGVWCDVFLA
                     LPGFPHDMSYAVGVLVFALGTVLCGVATGLYITAGLGAGPRDGFVLGVAKVTGAKVRT
                     VRTITELSVFALGWLMGGTVGLGTALFALTAGPLMQFFLRVFRGLGSAHAALEKRIAV
                     GHPARSAVSAAATETTRN"
     gene            complement(1556..>2329)
                     /locus_tag="PKN52_05425"
     CDS             complement(1556..>2329)
                     /locus_tag="PKN52_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HNQ99418.1"
                     /translation="FDAVYEIGRNFRNEGISYKHNPEYTMLELYWAGKDYLDILALVE
                     TMYSGLVQELLGTTKLTYQGTVLDFTPPWPRVDYTGELAKRAGFDFDPLDEPRLRAWA
                     AEKHPGAAAGEPPLAQRPLNQLFDKLYDIYVEPNLVGPVFVMDHPEAISPLAKRHRSR
                     PGLVERFEPVAVGMELGNAFSELNDPIDQRERFERQQALRDAGDDEAQMLDEDFLTAL
                     EYGMPPTGGLGLGIDRLAMLLADVPSIRDVILFPLLRPQ"
BASE COUNT          345 a          756 c          814 g          414 t
ORIGIN      
        1 cgaagatgtc gcccttcaac cagcgcccgg cctcgtccca gatctccccg accagatagg
       61 cgtcagggtt gacggcacgg cagcgggtcc ggaaggcgcg ccagaaatcg tcgtcgtcga
      121 tctcgttggg cacatccagg cgccagccgt ccatgccggc cgccagccac ttctcggcga
      181 cctggtagat gaactcgcgg actgccggcg tgtccgtgtt gaacttcggc agcgcggcga
      241 ggccccacca cgcgtcgtag ccgagctgac cgccgccgta ggcgtgcaag gggaaatggc
      301 gcacgtggaa ccagtcgagg tacgcgctgc tcgggccgtt ctccaggatg tcatggaact
      361 gcagcaggcc gcggctcgca tggttgaaca cgccgtcgag caccacgcgc atgccgcgct
      421 tgtggcaggc ggcgataagc tcgtcgaggg cggcgttgcc cccgagcatc ggatcgacct
      481 gatggaagtc ctgcgtgtga taacggtggt tggagccgga ggcgaagatc gggttgaggt
      541 agagagcggt cacgccgagg tcctgcaggt agtcgagctt ctccaccaca cctaggagat
      601 cgccgccctt gtagccgtgg agcgtcggcg gggcgtccca ctcctcgagg ttactgggct
      661 tgtgaacgcg cgaactacgt gcgaagcggt cggggaagat ctgatagaag acggcgtctt
      721 ggacccacgc cggcgagttg tcacttccag tgtcgcttcc agtgtcgctt ccaggcatgc
      781 cgggaatgct aacccggtgg tccgatcgga gttacgctcc gttggtgata cttccccggc
      841 gccccctttc ctggcgcgtc ttcctcctcc ggttactgca actcaacgtc gggctggtga
      901 tctacggcct gtccgttgct ctgatggtca acgccggcat cgggctgggc ccgtgggacg
      961 tcttccacca gggcctctcg cgcctgacgc ccctgaccat cggccaggcg atgatcctcg
     1021 ccgggctcgc catcctcgtc tactccgtca taggtccgaa ggtgaggatc ggcatagcca
     1081 ccgtactgaa catggtcctg atcggcgtgt ggtgcgacgt cttcctggcc ctcccgggct
     1141 tcccccacga catgagttac gcggtgggcg tactggtctt cgcgctcggc acggtgctct
     1201 gcggcgtggc caccggcctc tacatcacgg ccgggctcgg ggccggcccg cgagacggct
     1261 tcgtcctcgg cgtggccaag gtgacgggcg ccaaggtgcg cacggtgcgc accatcacgg
     1321 agctgtcggt gttcgcgctc ggctggctga tgggcgggac cgtggggctc ggcaccgcgc
     1381 tgttcgcgct cacggccggc ccactcatgc agttcttcct gcgggtgttc cgggggttgg
     1441 ggtcagcgca tgcggccctg gagaagcgga tagcggtcgg ccatcccgcc aggtccgcgg
     1501 tctccgctgc ggccaccgag acaacgcgga actaagcgct agcgtcccca accgttcact
     1561 gcggccgcaa caacggaaag aggatcacgt cccggatgct aggcacgtcc gccaacagca
     1621 tggccaggcg gtcgatgccg agcccgaggc cgcccgtcgg cggcatgccg tactccaggg
     1681 ccgtgaggaa gtcctcgtcg agcatctgag cttcgtcgtc gccggcgtcg cgcagcgcct
     1741 gctggcgctc gaaacgctcc cgctggtcga tggggtcgtt cagctcgctg aaggcgttac
     1801 cgagctccat gccgacggcc acgggctcga agcgctcgac gaggcccggg cggctccggt
     1861 ggcgcttggc gagcgggctg atggcctcgg ggtggtccat gacgaacacg gggccgacga
     1921 ggttgggctc gacgtagatg tcgtagagct tgtcgaagag ctggttgagg gggcgctgcg
     1981 ccagcggcgg ctcgcccgcc gcggcgccgg ggtgcttctc tgcggcccag gcgcgcaggc
     2041 gcggctcgtc gagcgggtcg aagtcgaacc cggcgcgctt ggcgagctct cccgtgtagt
     2101 cgacgcgcgg ccatggcggc gtgaagtcga gcaccgtgcc ctggtaagtg agcttggtgg
     2161 tgccgagcag ctcctgcacg agcccggagt acatcgtctc gaccagcgcg aggatgtcca
     2221 ggtagtcctt gcccgcccag tacagctcga gcatcgtgta ctcggggttg tgcttgtagc
     2281 tgatgccctc gttgcggaag ttgcggccga tctcgtagac ggcgtcgaa
//
LOCUS       DAOQYE010000279         2327 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_51877_length_2327_cov_2.556778, whole genome shotgun sequence.
ACCESSION   DAOQYE010000279 DAOQYE010000000
VERSION     DAOQYE010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51877_length_2327_cov_2.556778"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="PKN52_05430"
     CDS             complement(<1..301)
                     /locus_tag="PKN52_05430"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HNQ99419.1"
                     /translation="MSQDNEPRAATKAVWGGEEGVRIAGATQVPVVHSVAFGYDDLDV
                     WRDVALGRAEGHIYSRNTNPTVRAFEEKIRLLEGAEAATSFSTGMAAISATLFSLL"
     gene            complement(421..2064)
                     /locus_tag="PKN52_05435"
     CDS             complement(421..2064)
                     /locus_tag="PKN52_05435"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HNQ99420.1"
                     /translation="MRKRFLSLLLAAAFVALVGPSGAAFAQEHGQTGAQTSDQTSDQT
                     PDWPPPGWPPADGMPTDFPPDFQGGMPPFGGAQAQGQFGGPRGGGPGAPGGQGGGAGF
                     FGPNQQEYELVDEFDEDGDGVLNAAERAAALASIGRTVRTTVAGTPGPSLTPDDVTTY
                     PETAALYDPTAIRTIFLELDEENWEAELEAFYNTDVHLLGTVEVDGVTYENVGVRFRG
                     NSSYMMVAAGLKRPLRLKLDLVVDDQNLLGYRTLNLINGANDPSSLRTVIYSMIAQDY
                     IAVPKVGLVRLVVNGESWGIYQDQQQYNKDFLADYFDDTGGVRWKVSGSPNGQGGMRY
                     LGDDPDTYRSIYEIDNKDKPESWAALIDLFRTLNETPTENLVEALDPILDIDGVLRFF
                     ALEVALSNSDAFYSRASDYYIYLDEGGKFHVMPHDFNESLGAGERGPGGRGGSGSATT
                     IDPLVNVNDATKPLRTRLLAVPELRERYLAYVRDIAEKWLDWDTLGPIVAELHANIDA
                     DFAADTRALFGYEQFDASVGALKDYVEARQAYLLSVLPE"
     gene            complement(2057..>2327)
                     /locus_tag="PKN52_05440"
     CDS             complement(2057..>2327)
                     /locus_tag="PKN52_05440"
                     /inference="COORDINATES: protein motif:HMM:NF027640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HNQ99421.1"
                     /translation="VLEKEWGFRFTETHSVRYDNAALCSPLRRQDLLEDLRARTGLPV
                     RHVTVKQVDLLHDSALLQVHYYAPGEHAHGDDPARAGRALGDHHA"
BASE COUNT          325 a          774 c          825 g          403 t
ORIGIN      
        1 ccagcagcga gaagagcgtg gcgctgatcg ccgccatgcc ggtggagaag ctcgtggccg
       61 cctcggcccc ttctagcagc cggatcttct cctcgaaggc ccgcacagtc gggttcgtgt
      121 tgcggctgta gatatggcct tcggctctac cgagcgccac gtcgcgccac acgtcgagat
      181 cgtcgtaacc gaacgcgacg ctatgcacca ccgggacctg ggtagccccc gctatgcgca
      241 cgccttcctc gccgccccag accgccttgg tggcggcccg cggctcgttg tcttgactca
      301 cgccctgacc tcctcgagat ccgccgcccg cggttgccgc cgggctgcga ccttctcgtc
      361 agactcttca ggcagtgtag gacagcggcg accgctcggg tgccgctgtc ccttggggcg
      421 tcactccggc agcacggaca gcaaataggc ctgccgagcc tcgacgtagt ccttgagcgc
      481 gccgaccgag gcatcgaact gctcgtagcc gaacagcgcg cgggtgtcgg cggcgaagtc
      541 ggcgtcgatg ttggcgtgca gctccgcgac gatcggaccg agggtgtccc agtcgagcca
      601 cttctcggcg atgtcgcgga cgtacgccag gtagcgctcg cgtagctccg ggaccgcgag
      661 gaggcgcgtg cgcagcggct tggtcgcgtc gttcacgttg acgagcggat cgatggtcgt
      721 agccgagccc gagccgccgc ggccgcccgg cccccgctcc ccggccccga gcgactcgtt
      781 gaagtcgtgg ggcatgacgt ggaacttgcc gccctcgtcc aggtagatgt agtagtcgct
      841 ggcccggctg tagaacgcgt ccgagttgga tagcgcgacc tcgagggcga agaagcgcag
      901 gacgccgtcg atgtcgagga tggggtcgag cgcctcgacg aggttctccg tcggcgtctc
      961 gttcagcgtg cggaacaggt cgatcagggc cgcccagctc tccggcttgt ccttgttgtc
     1021 gatctcgtag atggaccggt aggtgtcggg gtcgtcgcca aggtagcgca tgccgccctg
     1081 cccgttcggg ctgcctgaca ccttccagcg cacgcccccc gtgtcgtcga agtaatcggc
     1141 gaggaagtcc ttgttgtact gctgctggtc ctggtagatc ccccagctct cgccgttcac
     1201 gaccagccgc acgaggccga ccttcggaac ggcgatgtag tcctgcgcga tcatcgagta
     1261 gatgaccgtg cggagcgagc tcgggtcgtt ggcgccgttg atgaggttga gggtgcggta
     1321 accgagcagg ttctggtcgt cgacgaccag gtcgagcttg aggcgcaggg gccgtttgag
     1381 gccggcggcc accatcatgt aagacgagtt cccgcggaac cgcaccccga cgttctcgta
     1441 agtgacgccg tcgacctcga cggtgccgag gaggtgcacg tcggtgttgt agaaggcctc
     1501 cagctccgcc tcccagttct cctcgtctag ttccaggaag atcgtgcgga tcgccgtcgg
     1561 gtcgtaaagc gcggcggtct ctgggtaggt agtcacgtcg tcgggcgtga gcgacgggcc
     1621 gggggtgcct gccacggtcg tgcgcaccgt cctcccgatc gaggctaggg cggccgcgcg
     1681 ctccgcggcg ttcaggacgc cgtcgccgtc ctcgtcgaac tcgtcgacga gctcgtactc
     1741 ctgctggttc ggtccgaaga agccggcgcc cccgccctga ccgcccggcg cgcccggccc
     1801 gccgccacgc ggcccaccga actggccttg ggcttgtgcg ccgccgaagg gcggcatgcc
     1861 cccctggaag tcgggcggga agtcggtcgg catgccgtcg gccggcggcc agcccggggg
     1921 cggccagtcg ggggtctggt cgctcgtttg gtcgcttgtc tgggcgcccg tttggccgtg
     1981 ctcctgggcg aacgccgcgc ccgaagggcc gacgagggcc acgaaggccg cggcgaggag
     2041 taacgagagg aagcgtttac gcatggtggt ctcctagcgc ccggcctgcc cgggcggggt
     2101 cgtccccgtg agcgtgctcc ccgggggcgt agtagtggac ttgcaggagg gccgagtcgt
     2161 gcaggaggtc gacctgcttg acggtcacgt ggcgtacggg caggcccgtg cgtgccctga
     2221 ggtcttcgag aaggtcctgc cgcctgagcg gcgagcacag ggccgcgttg tcgtaccgga
     2281 cggagtgggt ctcggtgaac cggaaccccc actccttctc caacacg
//
LOCUS       DAOQYE010000280         2322 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_52046_length_2322_cov_3.116012, whole genome shotgun sequence.
ACCESSION   DAOQYE010000280 DAOQYE010000000
VERSION     DAOQYE010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52046_length_2322_cov_3.116012"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            61..813
                     /locus_tag="PKN52_05445"
     CDS             61..813
                     /locus_tag="PKN52_05445"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF024744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HNQ99422.1"
                     /translation="MSTASPSHHDHRFIRLNGTLNTRDLGGLPVSGNGRTRFGVFFRS
                     DVPMDLQATDFERLAQLGLSTVIDLRQTHELERDKNSLAARSGIDYQNVEIWGHIEAA
                     GDQPTDPWDITAFYLRALDHAGSGFVRVMTLLANARGASLFHCTAGKDRTGLVAALLL
                     EAVGVERDTIIEDFALTHDRIDPLRKRLLEDADKRGVKRSDFERLLGATPDLMLPALD
                     YLATRYGGATAYLKANGLSDELLVTLKRKLVG"
     gene            981..1613
                     /locus_tag="PKN52_05450"
     CDS             981..1613
                     /locus_tag="PKN52_05450"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNQ99423.1"
                     /translation="MARPKAFDHDDTVLKAAGLFRRQGYEGTSVRHLLDELGLSASSF
                     YAEFGGKDALYMEALAAHAALEREQLRQGLSGPGTFKAKFAGLLAQLIDELTSVGGTS
                     SLTLRAAVEEAGTKPEVLAFLSRYLTELIEMVGDQIATAAERGELRLRHQPQPVARFL
                     LFGALSLGFVAKVSTDRATLDSYAAMVLDAMPGHATGLPGTPHQTQPPDA"
     gene            1661..>2322
                     /locus_tag="PKN52_05455"
     CDS             1661..>2322
                     /locus_tag="PKN52_05455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99424.1"
                     /translation="MERPFQAGPGMSAGARSRPDPDRRTGHIASTGQRPHGGRRRDLT
                     VVAAFVVLSIGFGLGALRLRLDASVSAMLPDKSAVAELQREVGDVFGADVVMVALLEG
                     DIYTEEAIAQLGQVTASLARVKGVTSVTSAANAQRMLDDDGFLLIEDLLPPAPTLDDL
                     AEARRYLETSPLYGGGLLVAEDGRSANVVMEVSADVDSAAMSNAIRAVLDEQWQRAGF
                     GAS"
BASE COUNT          375 a          852 c          766 g          329 t
ORIGIN      
        1 gacgggcacc tgcgccgtcg tcgcccgagc atgctacccc gatagtagaa tccccggacc
       61 atgagcacgg cgtccccttc acaccacgac caccgcttca tccggctgaa cggaaccctc
      121 aacacccgcg acctcggcgg cctccccgtc tccgggaacg gacgcacgcg cttcggggtg
      181 ttcttccgct ccgacgtccc gatggacctc caagcgaccg acttcgagcg cctggcgcaa
      241 ctcggcctca gcaccgtgat cgacctgcgc cagactcacg agctcgagcg ggacaagaac
      301 agcctggccg ccaggagcgg catcgactat cagaacgtcg agatctgggg tcacatcgag
      361 gcggccggcg accagcccac ggacccatgg gacatcacgg ccttctactt gagggccctc
      421 gaccacgcgg gctccggctt cgtccgcgtc atgaccctcc tggcgaacgc gcgcggcgcc
      481 tccctgttcc actgcacggc cggcaaggac cgcaccgggc tcgtcgcggc gctgctactg
      541 gaggccgtcg gggtcgaacg tgacacgatc atcgaggact tcgccctcac ccacgaccgc
      601 atcgacccgc tgcgcaagcg cctgctggag gacgccgaca agcgcggcgt caaacgcagc
      661 gacttcgagc gcctcctcgg ggccacccca gacctcatgc tgccggctct cgactacctc
      721 gccacgcggt acgggggcgc caccgcctac ctcaaggcca acggtctctc cgacgaactg
      781 ctggtcacgt tgaagcgcaa gctggtcggc tgaggcgcca gggcgcgggt cgtcgccgag
      841 agccgcgcgg cgcggccggg ctcggtgcca tgcgcgcact ccgtgctttc cgtaccgccg
      901 gccgggccgc gcgtcccggg aaccggtgat cgtaaggcag ttacggaaca aacgttccat
      961 aacgtgctag ccttggatcg atggcgcgac ccaaggcgtt cgaccacgac gacacggtgc
     1021 tcaaggcggc cgggctgttc cggcggcaag ggtacgaggg cacgtcggtg cgtcacctgc
     1081 tcgacgagct cgggctgagc gccagttcgt tctacgccga gttcggaggt aaggacgcgc
     1141 tctacatgga ggccttggcc gcgcacgccg cgctcgagcg cgagcagttg cggcagggcc
     1201 tctcgggccc cggcacgttc aaggcgaagt tcgcagggct gctcgcccag ctgatcgatg
     1261 aactgacgag cgtcggcgga acgagttcgc tgactctcag ggcggccgtc gaggaggcgg
     1321 gcacgaagcc cgaggtcctg gcgttcctct cgcgttacct gacggagctc atcgagatgg
     1381 tcggcgacca gatcgcgacc gcggcggagc ggggcgagtt gcgactgcgt caccagccgc
     1441 agcccgtggc ccgcttcctc ctcttcggcg ccctcagctt gggcttcgtc gccaaggtct
     1501 ccaccgacag ggcgacgctc gacagctacg ccgcgatggt cctcgacgcc atgcccggac
     1561 acgccacggg gcttccgggc acccctcacc agactcaacc ccccgacgcc taggaggcga
     1621 ccgaaggcat gatctccagc accgacaaca cacgccccat atggaacgac cgttccaggc
     1681 agggcccggc atgagcgccg gagcgcgtag ccgccccgac cccgatcggc ggacgggcca
     1741 cattgctagc accggccaga ggccccacgg cggccggcgg cgcgacctga cggtggtggc
     1801 ggccttcgtc gtcctatcca tcggcttcgg cctaggggcg ctgcgcctgc gcctcgacgc
     1861 ctccgtctcg gccatgctgc cggacaagag cgcggtcgcc gagttgcaac gggaggttgg
     1921 tgacgtgttc ggggcggacg tcgtcatggt cgcgctcctc gagggcgaca tctacacgga
     1981 ggaggccatc gcccagctcg gccaggtgac ggccagcctg gccagggtca agggcgtcac
     2041 gagcgtcacg agcgccgcca acgcccagcg catgctcgac gacgacggct tcctcctcat
     2101 cgaggacctg ctcccaccgg ccccgacgtt agacgacctg gcggaagccc gccgctacct
     2161 ggagacgtcg cccctctacg ggggcgggct gctcgtagcg gaggacggcc ggtcggccaa
     2221 cgtcgtcatg gaggtctccg ccgacgtcga ctcggccgcc atgagcaacg ccatccgcgc
     2281 cgtcctggac gagcagtggc agcgcgccgg cttcggggcg tc
//
LOCUS       DAOQYE010000281         2315 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_52294_length_2315_cov_2.907965, whole genome shotgun sequence.
ACCESSION   DAOQYE010000281 DAOQYE010000000
VERSION     DAOQYE010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52294_length_2315_cov_2.907965"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            287..1483
                     /locus_tag="PKN52_05460"
     CDS             287..1483
                     /locus_tag="PKN52_05460"
                     /inference="COORDINATES: protein motif:HMM:NF020932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imelysin family protein"
                     /protein_id="HNQ99425.1"
                     /translation="MYELRAVTPTSSARADQARTADARVAVTCPPAAYPAAAPRVATR
                     LRDAERASFLPKLALAAAVLTLCASLGLAWAQGVVGVKGYLLGQLATLQAGADDLAES
                     AKAYYDLAAAAGFDYQALAAGQQAAVAEALTGARDGWLTASPAYESVEGIVAGVELMA
                     QFDVDMDAGLSAEEGDEDLVSFDLTLPDGRVLVKPGNLFGVTESTLWATFDDFTTGVA
                     FDIDGDGAIGFGDSLPEANVLLAASEALAAMAADLTATAQTWEPTPADVFGALVGNVP
                     TVSEVFVARWRESRFVLGSATTMRDFGVISSLQDLNQNIASWRELYREVSPLVAAADA
                     TADADILAGLADLSAWADEIATAEADHTYTPEEADLIYDEGDRRATAITGAIAQAAAL
                     VGVEVE"
     gene            1495..>2315
                     /locus_tag="PKN52_05465"
     CDS             1495..>2315
                     /locus_tag="PKN52_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99426.1"
                     /translation="MVAFALALALAFLPFFGSAASAQGPLASSTDQVSVSSASLAEAV
                     RSSLASAQLYSGFAPNRAAEALARAETAWAELRGRFVSATGLREAGASQAPGQADEHS
                     TAAESVARVDAAFASARAALDLPDAERGPALAAARGALWTALLRGSMYATLAALDAGQ
                     PEPALALLGAREYRRATTVTTPGTEATSALLALQRGEMAAADARTAVSADLLDAYQAR
                     LLATIDDLAVARGAGQPVRVAELRSLAVGYFELLAPAYAEQLEVADGEGTQLGHP"
BASE COUNT          319 a          875 c          791 g          330 t
ORIGIN      
        1 ccgaggaccc aacccatgat cgaccagaag cacatcggct acgcgccgcc ccccgaaggg
       61 ggtgcgcgcc caccttccgt agtgccgagg tgggcgggag tcgccggtcg acgccgccgg
      121 tcagcgtcac ctgccgttga ctagccggtg gcgtagcagt agactccctc gattccctag
      181 ttggatactc ggatataggg ccgggtccga cgccgggcgc aggacagccg ccaggcatgg
      241 ggtcggagcc ggacgcccta gtcctggcca cacgatcgga gccaagatgt acgagctacg
      301 agcagtaacg ccgacttcca gcgcgcgagc cgaccaggcg aggaccgccg acgccagggt
      361 agccgtcacg tgcccgcccg ctgcctaccc ggccgccgcc ccccgcgtgg cgacgcgctt
      421 gcgcgacgcc gaacgggctt ccttcctgcc caagctggcc ttggcagccg ccgtcctgac
      481 cctgtgcgct agcctcggcc tcgcctgggc gcaaggcgtc gtcggggtga agggctacct
      541 cctcggtcag ctcgccacgc tgcaagccgg cgccgacgac ctcgccgagt cggccaaggc
      601 gtactacgac ctggcggcag cggccggttt cgactatcag gcgcttgccg ccggccagca
      661 ggccgcggtg gcggaggcgc tcacgggcgc ccgcgacggc tggttgacgg cgagccccgc
      721 ctacgagtcc gtcgagggca tcgtggccgg cgtcgagctc atggcgcagt tcgacgtcga
      781 catggacgcc ggcctctccg ccgaggaggg cgacgaggac ctcgtctcct tcgacctgac
      841 gctgcccgat ggacgcgtgc tggtgaagcc gggcaacctc ttcggcgtca ccgagtccac
      901 cctgtgggcc acgttcgacg acttcaccac cggcgttgcc ttcgacatcg atggtgacgg
      961 agccatcggc ttcggcgact ccctgcccga ggcgaacgtc ctgttggccg cgagcgaggc
     1021 gctcgccgcg atggcggccg acctgaccgc caccgcccag acctgggagc caacgcctgc
     1081 tgacgtcttc ggcgccctcg tcggcaacgt gcccacggtc tccgaagtgt tcgtcgcccg
     1141 ctggcgcgag tcgcgcttcg tcctcggtag cgccacgacc atgcgcgact tcggggtcat
     1201 ctccagcctc caggacctca accagaacat cgcctcatgg cgcgagctct accgcgaggt
     1261 ctccccgctc gtcgccgcgg ccgacgccac cgccgacgcc gacatcctgg ccggcctcgc
     1321 cgacctgagc gcctgggccg acgagatcgc gaccgccgag gcggaccaca cctacacgcc
     1381 ggaggaagcc gacctcatct acgacgaggg cgaccgccgg gccacggcca tcaccggagc
     1441 catcgcgcag gcggcggcgc tcgtgggcgt cgaggtcgag tagggcgtag ccatatggtc
     1501 gcgttcgccc tcgcgctcgc tctggccttc ctgccgttct tcggcagcgc ggcttcggcg
     1561 cagggtccgc tcgcgtcctc gaccgatcag gtcagcgtct cgtcggcgtc gttggccgag
     1621 gccgtccggt catcgttggc gtctgcccag ctgtactccg gcttcgcgcc gaacagggcg
     1681 gcggaagcgc tggccagggc cgagaccgcg tgggctgaac tccgcgggag gttcgtgtcg
     1741 gcgaccgggc tccgggaggc gggagcgagc caggcgcccg gtcaggcgga tgagcactcg
     1801 accgccgcgg agtcggtggc tcgggtcgac gccgccttcg cgtcggcgcg ggctgccctc
     1861 gacctgccgg acgccgagcg cgggcccgct ctcgccgccg cacgcggcgc cctctggacg
     1921 gccctgttgc gcgggagcat gtacgccacg ctcgcggccc tcgacgccgg ccaacccgaa
     1981 cccgctctag cccttctcgg cgcgcgcgag taccggcgcg ccaccacggt caccacgccg
     2041 ggcacggagg ctacctcggc gctcctagcg ctgcaacgcg gcgagatggc cgctgccgac
     2101 gcgcgcacgg ccgtctcggc cgaccttctt gacgcttacc aggcaagact gctcgccacc
     2161 atcgacgacc tggcggtcgc gcgtggggcg ggccaaccgg tgagggtggc cgagttgcgt
     2221 tccctcgccg tcggctactt cgagctcctc gcccccgctt atgccgagca gctcgaagta
     2281 gccgacggcg agggaacgca actcggccac cctca
//
LOCUS       DAOQYE010000282         2314 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_52327_length_2314_cov_3.129261, whole genome shotgun sequence.
ACCESSION   DAOQYE010000282 DAOQYE010000000
VERSION     DAOQYE010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52327_length_2314_cov_3.129261"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="PKN52_05470"
     CDS             complement(<1..384)
                     /locus_tag="PKN52_05470"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNQ99427.1"
                     /translation="MLAACIGVPAFAQNLTVWYAGNFQDQMDLVNNELVPAFEAANPG
                     IDLTVEFIPWGDLTAKLNTAFATGTVPDVFMHGQAATAGLVAADRLAPLDDYLAGFDV
                     ADFGPTFEQGSVDGKHYLAPVYGSGN"
     gene            816..1259
                     /locus_tag="PKN52_05475"
     CDS             816..1259
                     /locus_tag="PKN52_05475"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HNQ99428.1"
                     /translation="MAKQAEKRDLIAFAKAHGVRLTGQRRIILEVLAASDAHPDALEL
                     HRLVAKAHPSISLATVYRTVNLLEEKGVLEKHTFADGRSRYESTIGTHHHHHMINVET
                     GDVIEFSSEEFERLHAEICRKHGYEVVDHKLELYVRPIKRPTTDD"
     gene            complement(1262..2134)
                     /locus_tag="PKN52_05480"
     CDS             complement(1262..2134)
                     /locus_tag="PKN52_05480"
                     /inference="COORDINATES: protein motif:HMM:NF013143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HNQ99429.1"
                     /translation="MLEDLRAAFAFTFMQRALLAGSLIAVASGLLGTFVVQRRLAYLG
                     DGLAHAAFGGTGVGAFVLVATGQYGFRSEFLRHPLLIALPVALVTGLLITYVKRRTQL
                     SSDTTIGVFLAVAVAVGLFFFTRIPVDTPLGFDIMDVLFGSILAVGSTDLLAIAAITG
                     LAGLVLVRAWGRLAYATMDEELARSDGIDTARLEYVLVSITAAVVAASSVVVGVVLMT
                     AYLVIPAATARLSSITLLGMTVKAVVIGVVTTAAGLVGAFLFDAPTSSMLILVQAAVF
                     VVAAVGRSVVAARR"
     gene            complement(2134..>2314)
                     /locus_tag="PKN52_05485"
     CDS             complement(2134..>2314)
                     /locus_tag="PKN52_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99430.1"
                     /translation="ATAIFSESQLNRRPAEVLAAEAGVSLFELDPLGGVAGREGYEDL
                     LLWNAHVLLAAFGGE"
BASE COUNT          449 a          763 c          767 g          335 t
ORIGIN      
        1 gttgcccgaa ccgtagaccg gggcgagata atgcttgccg tccacgctgc cctgctcgaa
       61 cgtggggccg aagtcggcca cgtcgaaccc ggccaggtaa tcgtcgagcg gagcgaggcg
      121 atcggcggcc acgagccccg ccgtggccgc ctgcccgtgc atgaacacgt ccggcaccgt
      181 tccggtcgcg aaggccgtgt tgagcttggc cgtcaggtcg ccccagggta tgaactcgac
      241 cgtcaagtcg atgccggggt tcgccgcctc gaaggccggg acgagttcgt tgttgaccaa
      301 gtccatctgg tcctggaagt tccccgcgta ccagacggtc aagttctgcg cgaaggccgg
      361 cacaccgatg caggctgcta acaaggcgat cctaaggact aatctcactg ggttactcct
      421 agctgggtag gtgagccgac ggtagtgttg gctcttcacc atcgaagctc tggcttcggg
      481 gccgtaccgg tcgctggcgt gaccggttac gcgtacgtgg cgcgatatgg ctcctccttt
      541 cggctaaggt cacgggcgat caaaagatag ataaagcgct taattagttt tcgtacatca
      601 gggtagacga ccggtccgcg ctcgtcaagg ccaccgagga acggctcgga gaacacccgg
      661 ccggcaccct aagagacgag cgtcccctcg cccgagggct cctggcgaag ggacggcgag
      721 cccgagcggt ggggtcgaat cggctcacca gctcatggtc gaaagacgaa gcgcctggag
      781 cggtcatatc gacgggcgcg gtggtaacct tcgctatggc gaagcaggcc gagaagcgag
      841 acctcatcgc cttcgcgaaa gcgcatggcg tcaggctgac cgggcaacgg cggatcatac
      901 tcgaggtgct cgcggcatct gacgcgcacc cggacgcctt ggaactccac cggctcgtgg
      961 ccaaggcgca tccgagtatc tcgctcgcca ccgtctaccg caccgtcaac ttgctcgagg
     1021 agaaaggcgt gctggagaag cacaccttcg ccgacgggcg ctcaaggtac gagtcgacca
     1081 tcggcacgca ccatcaccac cacatgatca acgtggagac gggcgacgtg atcgagttct
     1141 cgtcggaaga gttcgagcgg ctacacgccg agatctgccg caaacacggc tacgaggtgg
     1201 tcgaccataa gctcgagctg tacgtgcgac ccatcaagcg cccgactacc gacgactgac
     1261 ctcagcgcct ggctgccacc accgagcgcc cgacggccgc aacgacgaag acggcggcct
     1321 gcacgaggat gagcatgctg ctggtggggg cgtcgaacag gaacgccccc accaggcccg
     1381 cggccgtcgt caccaccccg atgacgacgg ccttgacggt catcccgagc aacgtgatgg
     1441 agctgaggcg cgcggtcgcc gccgggatga cgaggtaggc ggtcatgagg acgacgccga
     1501 ctacgaccga gctggccgcc acgaccgccg ccgtgatgga gaccaggacg tactccagcc
     1561 tggcggtgtc gatgccgtcg gagcgcgcca gctcctcgtc catggtggcg tacgcgagcc
     1621 gcccccacgc ccgcaccagg acgaggcccg ccaagcccgt gatggcggcg atggccaaca
     1681 ggtcggtact gccaactgcc aggatgcttc cgaacaggac gtccatgatg tcgaagccaa
     1741 ggggcgtgtc gacggggatg cgcgtgaaga agaacaggcc gaccgccacg gcgaccgcca
     1801 ggaagacgcc gatcgtggtg tcgctggaga gctgcgtccg cctcttcacg tacgtgatga
     1861 ggagccccgt taccaacgcg acaggcaagg cgatcaggag cgggtggcgc aggaactcgc
     1921 tcctgaaacc gtactggccg gtcgccacga ggacgaaagc ccccaccccc gtccccccga
     1981 acgccgcgtg cgcgaggcca tcgccgaggt aggcgaggcg cctctgcacg acgaacgtgc
     2041 cgagcaggcc gctcgcgaca gctatcagcg agcccgccag cagcgcacgc tgcatgaacg
     2101 tgaaggcgaa cgcggcacgc aggtcctcca gcatcactcg ccgccgaagg ctgccaaaag
     2161 cacgtgggcg ttccagagga ggaggtcctc gtagccctcc ctcccggcca cgccgcctag
     2221 ggggtcgagc tcgaagaggc tcacgccggc ttcggccgcc aggacctcgg cgggtcgcct
     2281 gttgagctgc gactcgctga atatcgcggt ggcg
//
LOCUS       DAOQYE010000283         2314 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_52330_length_2314_cov_2.688800, whole genome shotgun sequence.
ACCESSION   DAOQYE010000283 DAOQYE010000000
VERSION     DAOQYE010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52330_length_2314_cov_2.688800"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            636..1247
                     /locus_tag="PKN52_05490"
     CDS             636..1247
                     /locus_tag="PKN52_05490"
                     /inference="COORDINATES: protein motif:HMM:NF013713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99431.1"
                     /translation="MTLHRRGEEYALRVANVELMNSRQHGSEEALATLAARAHGAPRS
                     VLVGGLGMGFTLRAVLDALTGDARVTVAELLPEVVEWNRTVLSHLAGNPLADERVLVV
                     VADVAELLKGAPGRYDMILLDTDNGPEGTTQDGNEWLYSAAGLATARAALAARGVLAV
                     WSAFESAGFAKRLARAGFAVGEERVRARGSKGARHVVWLARKA"
     gene            complement(1360..>2314)
                     /locus_tag="PKN52_05495"
     CDS             complement(1360..>2314)
                     /locus_tag="PKN52_05495"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HNQ99432.1"
                     /translation="LELQSLYGPRRRPTPATLARVDAVVIDLQDVGVRCYTYPTTAAL
                     VCEAAAEAGKRVVLCDRPNPLGPLMEGPMLEPARRSFIGYLDVPFRHGLSMGAAVALG
                     ARGSDADLVVAPATGWEPGSSGPVLPGAMPLPFVPPSPGLPTPEAVALYPGLVLLEGT
                     RLNEGRGTTLPFQLLGAPWLDGYELAGGLTELGIPGLRFRPVSYVPRSDTHAGETCHG
                     VHLLVEDGAALRPLQALVRILRHVRETHPEEFAWVAAADKPWSRLPGAGEPWHEPVAG
                     YLVDALAGDDSVRRVVDGEVTLEQAQTAWAAAAESWRRALG"
BASE COUNT          351 a          852 c          794 g          317 t
ORIGIN      
        1 ccacaggttg cgcctccccg ccgccgacca ggcgtcggcg aactcgccca tcggcgaggc
       61 gggcgttatg gggtagatgg cgatgacctc gttgaccttg tgcgccacca gggcggcggc
      121 ctcgttcccg tctactacgc tgcgcgtccc aggcatggcg gctcctttcc gctaccaaga
      181 ccgtgcccgg gtaaggcaac gagaccgcgc caccgtccgg gcctcatctc gaggctagcc
      241 gcaacgaggg agggcatatg gccccggaac cgcaccttcc gcgcaccgcc taccgtcgga
      301 gggcccggtc gtggccgggt tcggtgcgcc aagagccaac ttaggcgggt gtttcctgcg
      361 tcgagcggcg cgagaccgcc tatccggctg caaccccttg acgaatactg catacgggtg
      421 tatcttgaag acatcgaacg cgcgagagcg cgtttttttg ttgcccccga cgttagcacc
      481 ggcgccggtg cggctcgcgc caagccgctc agcggcagca ccaccgaccg cgggccttcc
      541 gcaaccgcca gacccgatca ctcgcgccac aggagcttga agacttgatc ccttgggaac
      601 cgatcggcga gaccgtcctc cctagcggcg agcgcatgac gctgcatcgc cggggcgagg
      661 agtacgcgct gcgcgtcgcc aacgtcgagc tcatgaacag ccgccagcac ggctcggagg
      721 aggcgctcgc cacgctggca gcgcgcgcgc acggcgcgcc gcgctcggtc ctggtgggcg
      781 gcctgggcat gggcttcacg ctcagggccg tgctggacgc tttgaccggt gacgcgcgcg
      841 tgacggtcgc agaactcctg ccggaggtcg tcgagtggaa ccgcaccgtc ctctcgcatc
      901 tggcgggtaa cccgctggcc gacgagcggg tgctggtagt ggtggcggac gtcgccgagc
      961 tcctgaaggg ggcgccgggt cgttacgaca tgatcctcct tgacaccgac aacggccccg
     1021 aaggtacgac ccaggacggc aacgagtggc tctactcggc ggcggggctt gcgaccgcgc
     1081 gcgccgccct cgcagcgcgg ggcgtgctgg ccgtgtggtc ggcgttcgag agcgccggct
     1141 tcgcgaagcg cctggcccgc gccggcttcg ccgtcggcga ggagcgcgtc agggctcggg
     1201 ggagcaaggg cgcgcgccac gtcgtgtggc tggcgcgcaa ggcctgaccc ggagtcctag
     1261 gccgccccac aactgctccc tcctggaagt tgctccacgc cgaacaggtc ggaaagggtg
     1321 aggcttctcc tcttcccagg ccaaccgagc gttcccaggc taaccgagcg cccgacgcca
     1381 actctcggcc gccgccgccc aagccgtctg cgcctgctcg agcgtcacct cgccgtcgac
     1441 cacgcgccgc acactgtcgt cgcccgccaa tgcgtcgacc aggtagccgg caacgggttc
     1501 gtgccacggc tccccagcac cgggcagcct gctccacggc ttgtcggccg ccgccaccca
     1561 ggcgaactcc tccgggtgcg tctcgcgtac gtgccgcagg atccgcacga gcgcctggag
     1621 cggacgcaga gccgcgccgt cctcgacgag caggtgaacg ccgtggcacg tctcgccagc
     1681 atgcgtatcg gaacgcggca catagctcac gggacggaag cgcaggccgg ggatgcccaa
     1741 ctcggtgagg ccgccggcca gttcgtagcc gtcgagccac ggtgccccga ggagttggaa
     1801 cggaagcgtg gtgccgcgcc cctcgttcag gcgagtgccc tccagcagca cgagacccgg
     1861 gtagagcgcg accgcctcgg gcgtcgggag ccccggcgac ggcggcacga acggcagtgg
     1921 catggcgcct ggcaggaccg gcccggacga cccgggctcc caaccggtgg ccggcgccac
     1981 caccagatcg gcgtcgctgc cccgcgcgcc caacgccacg gccgccccca tgctcagccc
     2041 gtgccggaac ggcacgtcga ggtaaccgat gaacgagcgt ctcgcaggct ccaacatcgg
     2101 cccttccatc agcgggccaa gggggttcgg acggtcgcac agcaccaccc tcttgccggc
     2161 ctcggccgcc gcctcgcaca cgagcgcggc cgtggtcggg taggtgtagc agcgcacgcc
     2221 cacgtcttgc aggtcgatca ccaccgcgtc cacccgggcg agggttgccg gggtcggccg
     2281 acgccgcggc ccgtacaggc tctgcaactc caac
//
LOCUS       DAOQYE010000284         2313 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_52359_length_2313_cov_2.954827, whole genome shotgun sequence.
ACCESSION   DAOQYE010000284 DAOQYE010000000
VERSION     DAOQYE010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52359_length_2313_cov_2.954827"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..942)
                     /locus_tag="PKN52_05500"
     CDS             complement(<1..942)
                     /locus_tag="PKN52_05500"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99433.1"
                     /translation="MPWPTEVDPAPREATEGYGLANRAFLVGGAVRDALLGRASTDRD
                     WLVGDPEVSARRFAEATGGAPFALDVERRHWRVAGPAGEVDDFVPLREGAASLEGDLR
                     LRDLTVNAMALSSAGALIDPTGGERDLRRRLVRMTSEDCLRADPIRPLRAARFATTLA
                     FELDEATRGAVTRRFEEQLRGAALMPAWERVGAELSAMLASPHAARGFALLKALGGLG
                     PYLPELEACRGVRQGGLHHLDVLDHQLEALNQLLHGFPDAGMALRLATLLHDIGKPAS
                     AALAPLGWKDAQAASASVSEDASAEPMADASAEPMADA"
     gene            953..1693
                     /gene="coaE"
                     /locus_tag="PKN52_05505"
     CDS             953..1693
                     /gene="coaE"
                     /locus_tag="PKN52_05505"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179029.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HNQ99434.1"
                     /translation="MSSRLGPVATRPSQESTVDPEASGAPDVVGTPDVAGARDVAGER
                     WRPLRVGLTGNIGTGKSSVARLLARHGAVVIDADALARQATDDPAVLERVAAELGADL
                     VKDGRLDRAATAARVFGDDRARSALNAIVHPWVAARRAELEAELASTEPRPRVVVHDV
                     PLLFETGLERDVDLVVVVSAPLAARVSRLAERSGLTEAEVLARDAAQLPLAEKMVRAD
                     YVVDNSGSEQDLEERVAHLWEELLARFP"
     gene            complement(1766..>2313)
                     /locus_tag="PKN52_05510"
     CDS             complement(1766..>2313)
                     /locus_tag="PKN52_05510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99435.1"
                     /translation="AAGISQFLSMAGAMASSFVDPMGEGGFVEPATRQVAGVTVSTYD
                     FGDGLAFETAVAGGYLLIGTKSGGLDSVLVSNASGGTGMATGLLASLQQPVPAGARSM
                     SLSDMRASIEALADEMVSQLGTVAGLTGGADLDFDAVEASSQAIAEYIHFIAQKVGGS
                     YSYQNVDGGAVRGYGLTQIAW"
BASE COUNT          315 a          818 c          857 g          323 t
ORIGIN      
        1 cgcgtcagcc atcggttcgg cagacgcgtc agccatcggt tcggcagacg cgtcctcgct
       61 tacggacgcg cttgcggctt gggcgtcctt ccagccgagc ggtgccaggg cggcgctggc
      121 cggcttgccg atgtcgtgaa ggagggtcgc taggcgcagc gccatcccgg cgtctgggaa
      181 gccgtgcagc agctggttga gcgcctcgag ctggtggtcg aggacgtcta ggtggtgcaa
      241 cccgccttgc cggacgccgc ggcaggcctc cagctcaggc agatagggcc cgaggccccc
      301 gagcgccttc aggagggcga agccgcgcgc cgcgtgcggg gaggccagca tggcggacag
      361 ttcggcgccg acgcgttccc aggccggcat gagcgccgcg ccccgcagct gctcctcgaa
      421 gcgcctcgtc acggcgccgc gcgtcgcctc gtccagctcg aacgcgagcg tggtcgcgaa
      481 gcgcgccgcc cgcagcgggc ggatggggtc ggcgcgcaga cagtcctcgg acgtcatgcg
      541 cacgagcctg cgacgcaggt cccgttcgcc accggtgggg tcgatgagcg ctcccgcgct
      601 gctcaaggcc atcgcgttga ccgtgaggtc gcgcaggcgg aggtcgccct ccaacgaggc
      661 ggcgccctcc cttagcggta cgaagtcgtc gacctccccg gccggccctg ccacgcgcca
      721 gtggcggcgc tcgacgtcga gcgcgaacgg cgccccgcca gttgcttccg cgaagcgccg
      781 cgcgctgacc tccgggtcgc ccaccagcca gtcccggtcg gtggacgctc tgccgagcag
      841 cgcgtccctg acggcgccgc cgaccagaaa ggcacggttg gccaggccgt agccctccgt
      901 cgcctcccgg ggtgccgggt cgacctccgt cggccacggc ataaactgac gcgtgtctag
      961 tcgactcgga cccgtcgcca cgcggccctc ccaggagtcc actgtagacc cggaggcgtc
     1021 cggcgcgccg gacgttgttg gtacgccgga cgttgcaggc gcgcgggacg ttgccggcga
     1081 gcgctggcgt ccgctccggg tcggactcac gggcaacatc ggcacgggca agtcgagcgt
     1141 ggcgcgactg ctggcccggc atggcgcggt cgtgatagac gcggacgcgc tcgcccgcca
     1201 ggcgacggat gacccggccg tcctggagcg ggtagccgcc gagctgggcg ccgacctcgt
     1261 caaggacggt cgcctcgacc gcgccgccac ggccgcgcgc gtcttcggcg atgaccgcgc
     1321 gcgctccgcg ctgaacgcga tcgttcatcc ttgggtggcc gcgcgccggg cggagctgga
     1381 agccgaacta gcctcgacgg agccacggcc gcgcgtggtg gtgcacgacg tgccgctcct
     1441 cttcgagacg ggccttgagc gcgatgtgga cctcgtggtg gtggtgagcg cgcccttggc
     1501 cgcgcgcgtg tcgcgcctcg ccgagcgttc cgggctaacg gaggccgagg tgctcgcacg
     1561 tgacgccgcc cagctcccgc tggccgagaa gatggtgcga gccgactacg tggtggacaa
     1621 ctcgggttcg gagcaggacc tagaggagag ggtggcgcac ctctgggagg agctactggc
     1681 tcgctttccc tgaaggtgcg ccaccctcga ataggcaggc ggtcgaaggc cggtggcggc
     1741 gcctaacgcg ccgccaccgc tccgctcacc aggcgatctg cgtcaggccg tagccgcgca
     1801 ccgcgccgcc gtcgacgttc tggtagctgt aggagccacc gaccttctgc gcgatgaagt
     1861 ggatgtactc ggcgatggcc tggctggagg cctcgacggc gtcgaagtcg aggtccgcgc
     1921 cgcccgtcag gccagcgacc gtgccgagtt gggacaccat ctcgtcggcc aacgcctcga
     1981 tgctcgcgcg catgtctgac aggctcatgc tcctggcgcc ggcgggaacg ggctgctgca
     2041 gcgaggcgag caggcccgta gccatcccgg tgccgcccga ggcgttggag acgaggacgc
     2101 tgtcgaggcc acccgacttg gtgccgatga ggaggtaccc gcccgccacg gcggtctcga
     2161 aggcgaggcc gtcgccgaag tcgtaggtgc tgaccgtgac gccggctacc tggcgcgtcg
     2221 cgggctcgac gaacccgccc tcgcccatgg ggtcgacgaa cgaggaggcc atcgctccgg
     2281 ccatgctgag gaactgggag atgcccgcgg cgg
//
LOCUS       DAOQYE010000285         2303 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_52683_length_2303_cov_3.149466, whole genome shotgun sequence.
ACCESSION   DAOQYE010000285 DAOQYE010000000
VERSION     DAOQYE010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52683_length_2303_cov_3.149466"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..864
                     /locus_tag="PKN52_05515"
     CDS             <1..864
                     /locus_tag="PKN52_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ99436.1"
                     /translation="VVRFSGDPLAIYLGDDDMVVGQVGNDQIAALRARLGLDQPLPLQ
                     YIYFLRDALHGDFGRSFIYQNQPALPLVIRRLPATAALGGAALLIGILISLPAGLIAA
                     RFRNRFPDTFASFYAVVGEAVPNFWLGIMLILLFAVNLRVLPVSGSGTWRHLVLPAFT
                     LGTGMSALLMRLLRGNLIEVMSLDYIRTARAKGLSARSVLFKHGLRNAAISYITVLGL
                     SVPGLLSGSVVTESIFAWPGMGLLFITAINGRDMAVVQAIVVFTAFLAILGNLIVDLV
                     YVLVDPRITYR"
     gene            861..1760
                     /locus_tag="PKN52_05520"
     CDS             861..1760
                     /locus_tag="PKN52_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ99437.1"
                     /translation="MTHAGPTAERAPRRGAGLRRAWRKLLRNPPAIFGALVLVVFVFI
                     ALFAPLVAPHNPRQQNLSDRFTPPVWDKAGDTRYLLGTDQLGRDILSRLIYGSRVALA
                     VGVFGTGLALVIGVTLGLCAGYFGGRVDAIVTRVTEVIISIPSSILYITILGVFGPSL
                     WLLILVMGLFGWITSARVVRGEVLSIRNREYVEASIGLGQRTARVLIKHVLPNVLGSI
                     IVVGTLKVASMIILEASLSFLGFGVQPPAVTWGQMLSDGRRFITSSWWLATLPGLAIS
                     LLTLSLLFLGNWLRDNFDPRTYD"
     gene            1770..>2303
                     /locus_tag="PKN52_05525"
     CDS             1770..>2303
                     /locus_tag="PKN52_05525"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HNQ99438.1"
                     /translation="MKTAEAPYPKKEVVARDAIVAACDERAADWGVEVMKQGGNAVDA
                     AVVTGFLMMVYEPFSCGLGGHGGHMAFYDGTTGEVVGIDASSKAPAKAHADMFKDELL
                     EVPEFYGDVDLVKVKDDANALGFRAMYAPTTVAQYHYVQERFGTWSWEQVLAPAIKVA
                     EEGFVADEIYTRRMNATR"
BASE COUNT          360 a          810 c          722 g          411 t
ORIGIN      
        1 gtggtcaggt tctccggcga cccgctcgcc atctacctgg gggacgacga catggtggta
       61 gggcaggtcg ggaacgacca gatcgccgcc ttgcgcgccc ggctcggact cgaccagccg
      121 ctgccgctcc agtacatcta cttcctgagg gatgccctgc acggagactt cggtcggtcg
      181 ttcatctacc agaaccaacc cgccctcccc ctggtgatcc gccgcctacc ggcgacggcc
      241 gcgctgggag gagccgcgct gctgataggg atcctcatca gcctgccggc cgggctgatc
      301 gcggcccgct tccgcaaccg cttcccggac accttcgcca gcttctacgc ggtcgtcggg
      361 gaggcggttc ccaacttctg gctcggcatc atgctgatcc tcttgttcgc cgtcaacctc
      421 agggtcctcc ctgtatccgg ctcgggcacc tggcggcacc tcgtgttgcc cgccttcacg
      481 ctcggcaccg gcatgtcggc gctgctgatg aggctgctgc ggggcaacct gatcgaggtc
      541 atgagcctcg actacataag gacggccagg gccaagggcc tctctgcgcg ctcggtcctc
      601 ttcaaacacg gcctacgcaa cgccgctatc tcctacataa cggtcctcgg cctgtccgtc
      661 cccggtctgc tgtccggatc cgtcgtgacc gagagtatct tcgcttggcc cgggatgggc
      721 ctcttattca taaccgcgat caacgggcgc gacatggcgg tcgtccaagc catcgtcgtg
      781 ttcaccgcgt tccttgccat cctcgggaac ctgatcgtcg atctcgtcta cgtcctcgtc
      841 gaccccagga tcacgtaccg atgacgcacg ccggtccgac cgcggaacga gcccccaggc
      901 gcggagccgg cctacgccgc gcctggcgca agctcttgcg caacccgccc gccatcttcg
      961 gcgcgctggt gctggtcgtg ttcgtgttca tcgccctctt cgcaccgctc gtcgcccccc
     1021 acaaccccag gcagcagaac ctcagcgacc ggttcacgcc gccggtgtgg gacaaggccg
     1081 gcgacacccg ctacctgttg ggcacggatc agttgggccg ggacatcctg agcaggctca
     1141 tctacggatc gcgggtggcc ttggccgtcg gcgtgttcgg gacgggcctg gcgctcgtga
     1201 tcggcgtgac gctcggcctg tgcgccgggt acttcggggg cagggtagac gcgatcgtca
     1261 cccgcgtcac cgaggtgatc atcagcatcc cgagcagcat cctctacatc accatcctgg
     1321 gcgtcttcgg cccgagcctc tggctcttga tcctcgtcat gggcctcttc gggtggatca
     1381 cctcggccag ggtggtgaga ggcgaggtgc tctccatccg gaaccgcgag tacgtcgaag
     1441 cgtccatcgg cctcggacag cgcaccgcgc gcgtgctcat caagcacgtg ctgccgaacg
     1501 tgcttggctc catcatcgtc gtcggcacct tgaaagtggc gagcatgatc atcctcgagg
     1561 cgagcctaag cttcctcggc ttcggcgtcc aaccgcccgc ggtcacctgg gggcagatgc
     1621 tctccgacgg tcgccgcttc atcaccagct cctggtggct ggcgacgctg cccggcctcg
     1681 cgatctccct cctgacgctg agcctgttgt tcctgggtaa ctggctgcgt gacaacttcg
     1741 accccagaac ctacgactag gagaccagca tgaagacggc cgaagccccc tacccgaaga
     1801 aggaagtagt cgcccgcgac gcgatcgtag ccgcctgcga cgaacgcgcc gccgactggg
     1861 gcgtcgaggt gatgaagcag ggcggcaacg ccgtggacgc cgccgtagtc accgggttcc
     1921 tcatgatggt gtacgagccg ttctcgtgcg gcctaggcgg ccacggcgga cacatggcct
     1981 tctacgacgg cacgaccggc gaggtggtcg gcatagacgc ctccagcaag gcgccggcga
     2041 aggcgcacgc cgacatgttc aaggacgagc tactcgaggt gccggagttc tacggcgacg
     2101 tcgacctcgt gaaggtcaag gacgacgcca acgccctcgg cttccgcgcc atgtacgcgc
     2161 ccaccaccgt ggcgcagtac cactacgtgc aggagcgctt cgggacgtgg tcgtgggagc
     2221 aggtgctcgc cccggccatc aaggtcgcgg aggaggggtt cgtcgccgac gagatctaca
     2281 cgcggcgcat gaacgctacc cgg
//
LOCUS       DAOQYE010000286         2298 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_52863_length_2298_cov_3.672760, whole genome shotgun sequence.
ACCESSION   DAOQYE010000286 DAOQYE010000000
VERSION     DAOQYE010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2298
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52863_length_2298_cov_3.672760"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="PKN52_05530"
     CDS             complement(<1..325)
                     /locus_tag="PKN52_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99439.1"
                     /translation="MDIKSLDGETYVPEFNGNRKLPKGEQVTVTLRYPTIEQYEPFAG
                     SRDKDPDALGLIRFAVTGIRNLAENGKAIETGGDLVACRRGLMSDLVNELFVHILTVN
                     RMSGAQ"
     gene            complement(390..1040)
                     /locus_tag="PKN52_05535"
     CDS             complement(390..1040)
                     /locus_tag="PKN52_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99440.1"
                     /translation="MAEKRLGGSKNYLYFGELGTEVTTGALSGEKFFKITAKGASSAF
                     PATSVVGDVIYNKPAITLATGDKAKPVTLTKLGFVTNVPQSASKEKFEDTVQTDDVKS
                     YSEGDKPEITGNVEGYFVAEAEKIDEILNRFFRVVSDDGAGAVTYAPTTTGVVHLFLG
                     RNETTVVGEIEIMEYMPSIVESLTVDKPMEGKQTFGFAYTVVGSERPSIYNRTITA"
     gene            complement(1042..1497)
                     /locus_tag="PKN52_05540"
     CDS             complement(1042..1497)
                     /locus_tag="PKN52_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99441.1"
                     /translation="MAKLRTHEALLAIRSYLVSGIPVEMAAAGGTSRIQDWIIGRYDP
                     ERLLAYDALLIGTIPERVGALDDEQTIRMFLDLAFAVRGSTEAEVIERQLRYGDALVN
                     LVQDDRTLGGAVFGSTVYNPEPEMPAPGAKMTGLTVVRLQIELNLLGGE"
     gene            complement(1475..1969)
                     /locus_tag="PKN52_05545"
     CDS             complement(1475..1969)
                     /locus_tag="PKN52_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99442.1"
                     /translation="MMRMSLARVNKGALKRFAGRMPKQANFIISKIASRFAEFVREQY
                     LSGQVLRERTQTERARSARRGKPVRTRSTKESTRFFKIGVGHTRGVGSARDAIRFGVR
                     PGSRIRGRLNYLMRFERGERPFMEPAYRDFRRRKMHSQIAQGYMDRFIREENRRSSGQ
                     AANA"
     gene            complement(1966..>2298)
                     /locus_tag="PKN52_05550"
     CDS             complement(1966..>2298)
                     /locus_tag="PKN52_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99443.1"
                     /translation="VYMDALGVFGSGTEITDAVLYGEAGILARRAGLFPRGLQNVRVQ
                     YTAGMDPVDERLQFAAIEVVAWNLQRFRGAGGVGLRSAKVDDWDYSYEITIPLSAQRV
                     FESYRSGA"
BASE COUNT          333 a          825 c          691 g          449 t
ORIGIN      
        1 cctgcgcccc gctcatccgg ttgacggtca ggatatggac gaagagctcg ttgactaggt
       61 cgctcatcag cccacgccgg catgccacga ggtccccccc ggtctcgatg gccttcccgt
      121 tctccgcgag gttccggatc cccgtgaccg cgaacctgat caggccgagg gcgtcggggt
      181 ccttgtcgcg gcttcccgcg aacggctcgt actgctcgat cgtcgggtag cgcagcgtga
      241 ccgtgacctg ctcgcccttc gggagcttcc tgttcccgtt gaactccggg acgtacgtct
      301 caccgtccag gctcttgatg tccacgttct cctccctgcg cctcggcgaa gccgcccggg
      361 gaggccccag ggtcggcgct cgccggctct cacgccgtga tcgtgcggtt gtagatgctc
      421 ggccgctcgg agcccaccac ggtgtaggcg aacccgaagg tctgcttccc ctccatgggc
      481 ttgtccacgg ttaggctctc gacgatcgag ggcatgtact ccatgatctc gatctcgccc
      541 acgaccgtcg tctcgttcct cccgaggaag aggtggacga ctcccgtggt cgtcggggcg
      601 taggtcactg cgccggcgcc gtcgtcggag acgacccgga agaagcggtt caggatctcg
      661 tcgatcttct ctgcctccgc cacgaagtag ccctcgacgt tccccgtgat ctccggcttg
      721 tctccctcgg agtagctctt cacgtcgtcc gtctgcaccg tgtcctcgaa cttctccttc
      781 gaggcgctct gcgggacgtt ggtgacgaaa ccgagcttcg tcagcgtgac cggcttcgcc
      841 ttgtcgccgg tggccagcgt gatcgccggc ttgttgtaga tcacatctcc caccaccgag
      901 gtggccggga aggcgctcga ggcgcccttg gccgtgatct tgaagaactt ctcgcccgac
      961 agggcgccgg tggtcacctc cgtgcccagc tctccgaagt acaggtagtt cttcgatcca
     1021 cccagtctct tctccgccat gtcactcccc tcccagtaga ttgagctcta tctgcagcct
     1081 cacgactgtc agtcccgtca tcttcgcccc cggtgccggc atctccggct ccgggttgta
     1141 gaccgtgctt ccgaagaccg cgccgcccag ggtccggtca tcctgcacca ggttcacgag
     1201 agcatcgccg taccgaagtt gtcgctcgat gacttcggcc tccgtcgagc ctcggacagc
     1261 gaaggccagg tcgaggaaca tccggatcgt ctgctcgtcg tcgagcgccc ccacccgctc
     1321 cgggatcgtt ccgatgagga gcgcgtcgta ggcgagcagt cgctccgggt cgtagcgtcc
     1381 gatgatccag tcctggatcc ggctcgtccc tcccgcggcc gccatctcca cggggatccc
     1441 gctcacgagg tacgagcgga tggccagaag cgcctcatgc gttcgcagct tggccactcg
     1501 acctcctgtt ctcttcccgg atgaagcggt ccatgtagcc ctgggcgatc tggctgtgca
     1561 tcttccggcg gcggaagtcc ctgtaggcag gctccatgaa cggccgctcg ccccgctcga
     1621 agcgcatgag gtagttgagc cgaccccgga tccggctccc aggtcgcact ccgaaccgga
     1681 tggcgtctcg ggcgctcccc acccctcgcg tgtgtcccac gccgatcttg aagaatcgcg
     1741 tcgactcctt cgtcgaccga gtccggaccg gcttcccccg gcgtgcgctc ctggcccgct
     1801 ctgtctgggt gcgctcccgc aggacctggc cgctcaggta ctgctcgcgg acgaactcgg
     1861 cgaagcgcga ggcgatcttc gagatgatga agttggcctg cttgggcatg cgcccagcga
     1921 accgcttgag cgcccccttg ttcacccgcg cgaggctcat ccgcatcatg cgccgctccg
     1981 gtagctctcg aagacccgct gagcgctcag cgggatcgtg atctcgtagg agtagtccca
     2041 gtcgtcgacc ttggccgatc tcaggccgac gccaccggcg ccacggaacc gctgcaggtt
     2101 ccaggccacg acctcgatcg cggcgaactg cagccgttcg tccactgggt ccatgcccgc
     2161 ggtgtactgc acacggacgt tctggaggcc tcggggaaac agacccgcgc ggcgggccag
     2221 gatccccgct tctccataga gcacggcatc cgtgatctcc gtcccggacc cgaaaacgcc
     2281 cagagcgtcc atgtagac
//
LOCUS       DAOQYE010000287         2296 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_52959_length_2296_cov_1.940651, whole genome shotgun sequence.
ACCESSION   DAOQYE010000287 DAOQYE010000000
VERSION     DAOQYE010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52959_length_2296_cov_1.940651"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="PKN52_05555"
     CDS             complement(<1..387)
                     /locus_tag="PKN52_05555"
                     /inference="COORDINATES: protein motif:HMM:NF013109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HNQ99444.1"
                     /translation="MLTSSESNRPSSKLTRFLPKATDYKPKYWRVDVLAGVTVAFVAL
                     PLALGFGVTAGAGATAGIITAIVAGVVAAFFGGSAFQVTGPTGAMTAVLLPIVAHYGP
                     GALPILGIGAGLILIVMGVAGVGRYVR"
     gene            591..1346
                     /locus_tag="PKN52_05560"
     CDS             591..1346
                     /locus_tag="PKN52_05560"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HNQ99445.1"
                     /translation="MSTDYLTDLLLVVPHPDDEVFGVGAMLARMADCGRVATLTLTRG
                     AAGRTLGLATRAELPNVRERELRASLAALGVQDVTILDYQDYVPDDERGMPRHPGLVG
                     ADRATLLGEIEAVLARTRPRVLFTFAPNGSNGHPDHWLTSELTFEAFDRAEVRPEKLY
                     CFASPQPFRGPQREGFLPEDEMLRRAVPVTHVAEAGSELIKKLAAMACHETQALSVLG
                     FMRSNPARLITETFHQVYPPVPACAEVVRVEKL"
     gene            1474..>2296
                     /locus_tag="PKN52_05565"
     CDS             1474..>2296
                     /locus_tag="PKN52_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99446.1"
                     /translation="MLFRRQRAPAPDTVTASAQEALARGQYDVAFAMLESAVRRGTNR
                     SGANRAEQGQTWLQLAAVYALYGDEGLENGQPALRSAVAAEPKLASHPLYRALFWEFA
                     AYRGAPLADVKRGLREVPEHAVDGMPGSFDVVAAYHAAAALFAVDAAKSARRRLRAVP
                     EGGLPAYLEWRRWSLLGQCCETLGEWEEAADAFAMAVDQAPDQERHGALGDPHRLPDR
                     QSHLIQGRARAQLDLALGIHPGDQGAFGLGANGDRVGAGGLSLHLAVPAVEGLKDH"
BASE COUNT          345 a          799 c          825 g          327 t
ORIGIN      
        1 gcgtacgtaa cgacccacgc ccgcgacgcc catgactatg aggatgagtc cggcgccgat
       61 gccgaggatg ggtagggcgc ccggcccgta gtgggcgacg atggggagca ggacggccgt
      121 catggcgccc gtcgggccgg tcacctggaa cgcggagccg ccgaagaacg ccgccaccac
      181 gccggcgacg atggccgtga tgatgccggc ggtagcgccg gccccggccg tgacgccgaa
      241 gccgagcgcg agcggcaggg cgacgaacgc gacggtgacg ccggccagca cgtctacccg
      301 ccagtacttc ggcttgtagt cggtggcttt gggtaggaaa cgggtgagct tgcttgaggg
      361 gcggttcgac tcgctgctgg tgagcaccgg cggagcacct tccttggaac cgggcgcgac
      421 ttggcgcgcc ccgtgccaga tgatgagcca ctccgcgccg cgcggcaagt cccctgggcg
      481 gcgagagtta caaagcagat gcgccaccgg cgcaggtccc gccctacggg accggtggat
      541 gggacgggcc gctgacgcga ccctccagcg acagggttac gctggtccgc atgagcacgg
      601 actaccttac cgacctcctg ctcgtcgttc cccaccctga cgacgaggtc ttcggtgtcg
      661 gcgccatgct ggcacgcatg gccgactgcg gccgcgtggc gacgctgacg ctgacgcgcg
      721 gcgccgcggg caggaccctg ggcctcgcca cgcgcgccga gctgccgaac gtgcgcgagc
      781 gcgagctccg cgcctcgctc gccgcgctcg gcgtccagga cgtgacgatc ctcgactacc
      841 aggactacgt gccggacgac gagcgcggca tgcctcgtca cccagggctc gtgggggccg
      901 accgagcgac gctgctcggc gagatcgagg ccgtgctggc ccggacacga cctcgcgtcc
      961 tcttcacgtt cgcacccaac ggctcgaacg gtcacccgga tcactggctg acgagcgagc
     1021 tcacgttcga ggcgttcgac cgcgccgagg tgcggccaga gaagctctac tgcttcgcca
     1081 gcccgcaacc gttccgcgga ccccagcgcg agggcttcct gccggaagac gagatgctga
     1141 ggcgcgccgt gcccgtcacg cacgtcgccg aggccggctc cgagttgatc aagaagctgg
     1201 cggcgatggc ctgccacgag acgcaggcgc tgtcggtcct cggcttcatg cgttccaacc
     1261 ccgcgaggct gataacggag accttccacc aggtgtaccc gcccgtgccg gcgtgcgccg
     1321 aggtggtgcg ggtggagaag ctttagatca cttggaggcc gtccagcaca cgcggatcgc
     1381 ggacgcggaa gcgcgtgctc ggctcgccac cgtcgctgac gagcagtgtg gccgaattcg
     1441 tgtccctgac ggacgtgcat ggtagcgtta cgcatgctgt tcaggcgtca acgggcgccg
     1501 gccccagaca cggtgacggc ctcggcgcag gaagcactcg cgcgaggcca gtacgatgtg
     1561 gcgttcgcca tgctagagag cgccgtgcgc cgcggtacca atcgcagtgg ggccaaccgc
     1621 gcggagcagg gccagacctg gctgcagctc gccgccgtgt acgccctgta cggcgacgaa
     1681 gggctcgaga acggtcagcc ggccctcagg agcgcagtgg cggccgagcc gaagctcgcc
     1741 tcgcaccccc tgtaccgcgc cctcttctgg gagttcgccg cctaccgcgg cgcgccgctt
     1801 gccgacgtca agcgtggcct gcgtgaggtc ccggagcacg ccgtcgacgg catgcccggt
     1861 tcgttcgacg tggtggccgc ctaccacgcg gcggccgcgc tcttcgcggt cgacgcggcc
     1921 aagagcgcac ggcgcagact gcgggcggtg ccggagggcg gcctgcccgc ctacctcgag
     1981 tggcgccgct ggtcgctcct cggccagtgt tgtgagacgc tcggcgagtg ggaggaggcg
     2041 gccgacgcgt tcgccatggc cgtcgaccag gcccccgatc aagagcggca tggtgccctc
     2101 ggggacccgc accgtctccc ggaccggcag tcccacttga tccagggccg ggctcgggcc
     2161 cagctcgacc tggccctcgg gatccatccg ggtgaccaag gcgctttcgg gttgggtgcg
     2221 aatggtgacc gggtaggtgc gggtggactc tcgctccacc tcgctgttcc agccgtagag
     2281 ggtctgaaag accacg
//
LOCUS       DAOQYE010000288         2280 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_53520_length_2280_cov_2.904270, whole genome shotgun sequence.
ACCESSION   DAOQYE010000288 DAOQYE010000000
VERSION     DAOQYE010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53520_length_2280_cov_2.904270"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1512)
                     /locus_tag="PKN52_05570"
     CDS             complement(<1..1512)
                     /locus_tag="PKN52_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99447.1"
                     /translation="MAQAYRYPLTRVCLRGGTMTLPLAMLELFSGSEAIVAIDLETGK
                     EYPLELVSRRQLAGLGSLYREHGLDVNDELHVTPVGEGRFSFEAVSKAKRAAQDATTA
                     LTAILDELAEAGVAATEAELHALYPALPEGFDLTPYLSADPRFEFRNGRWQSAAVGSP
                     DGDQAQRDAAEAHVSETPRMVATVSEEVAELVETAPAGQIMQAVAGGHHQPRGGEPLA
                     GAVAGMGAPDPAQPGLWGGEGRADASRHAEAPARRVHERRVPGSSGEPAEPRGVLDDT
                     DLVALDLSKRLRGVLEPLGFKLTPLGGGQLTIATDMGRRNYQVHAMLLPEGERLDWAA
                     LLSRRRSSNARYLAVIGDHRDLVRLVSPAELARATLWSWAALERLRVLHGTVPLTPVD
                     LESHFERDGLFEAGLARFERGVTERVAERGATSQVLTRLAQLKAPSVFLLEELATDVG
                     IGREAVLRILERLSEAPFQLVARVEQGEFLLRLPVATALSSLAAYADSLRERLP"
     gene            complement(1723..>2280)
                     /locus_tag="PKN52_05575"
     CDS             complement(1723..>2280)
                     /locus_tag="PKN52_05575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99448.1"
                     /translation="LMHVSHLVDDYYLYIEDQLMLMGKHTRKRYRMGDRVEVKILAAN
                     PTQRQIDLLPADLPMPEVEEEKQADTRPPGKLKTPQQGAAEGVVATSGSTKGASAKGA
                     PAKNGSAKGGSAKSGSADGGSAGSGPAKGARSDGRGAPAGRKAAGPRRVPPRRHQRPS
                     LPPRLRQSRPQRRPRRDRRPRRNAR"
BASE COUNT          308 a          798 c          811 g          363 t
ORIGIN      
        1 cgggaggcgt tcccgcagtg aatcggcgta ggcggccagg ctgcttaggg ccgtggccac
       61 gggcaggcgc agcaggaact cgccctgctc cactcgcgcc accaactgga acggggcctc
      121 cgacagacgc tcgaggatgc gcagcacggc ttctctgcct atgccgacgt cggtagcgag
      181 ctcctcgagg aggaagacgc tcggggcctt gagctgcgcg aggcgcgtca gcacctgcga
      241 ggtggcgccg cgctccgcga ctcgctcggt gacgccgcgc tcgaaccgcg cgagccctgc
      301 ctcgaagagg ccgtcgcgct cgaagtgcga ctcgaggtcg acgggtgtga gtgggaccgt
      361 cccatgcagc acccgcaggc gctcgagggc ggcccaactc cagagcgtgg cacgcgcgag
      421 ttcggccggg ctgacgagcc ggacgaggtc acgatggtcg ccgatgacgg cgaggtaacg
      481 ggcgttggat gaccgacgcc gcgagagaag ggccgcccag tcgaggcgct cgccctccgg
      541 cagcagcatg gcgtgcacct ggtagttgcg gcgccccatg tcggtggcga tggtgagctg
      601 gccgccgccc aacggcgtca gcttgaagcc gagcggctcc agcacgccgc gcaggcgctt
      661 cgagaggtct agggcgacga ggtcggtgtc gtcaagcacc ccgcgcggtt cggcaggctc
      721 gccggaactc ccaggaactc ggcgctcgtg gacgcgcctg gcaggcgcct cggcatgccg
      781 cgacgcgtcg gcgcgacctt ctccgcccca gaggccgggt tgcgcagggt ccggcgcgcc
      841 catgccggcg acggctccgg cgagcggctc gcccccacgc ggctggtggt gaccgccggc
      901 aacggcctgc atgatctggc cggcgggcgc ggtctcgacg agctcggcca cctcctcgct
      961 gaccgtggcg accatgcggg gcgtttccga cacatgggct tccgccgcat cgcgctgcgc
     1021 ctgatctccg tccggcgacc cgaccgcggc gctctgccac cgcccgttgc ggaactcgaa
     1081 gcgcgggtcg gcagagaggt agggggtgag atcgaaccct tccggcaaag cggggtacaa
     1141 cgcgtgaagc tcggcctcgg tggccgccac gccggcttcg gccagttcgt cgaggatcgc
     1201 cgtgagagcg gtggtggcgt cttgcgcggc cctcttggcc ttgctgacag cctcgaagct
     1261 gaaccggccc tcgcctacgg gtgtgacgtg aagctcgtcg ttgacgtcta gaccgtgctc
     1321 gcggtagagg gaccccagcc cggcgagttg ccgccgcgag accagttcga gcgggtactc
     1381 cttgcccgtc tcgaggtcta tcgcgacgat ggcttcgctc cctgagaaca actccaacat
     1441 ggcgagtggc agggtcatgg tgccgccgcg gaggcagacg cgggtgagcg ggtaccgata
     1501 tgcctgtgcc atgtcgtgtc tcctaaatcg tccagctggg cgagccccgg ggcggaaccg
     1561 acgtcgggca gccgtcttcc ttgatccgaa ggtcctggcg ccagccggtc ggttggcgaa
     1621 gtctaccaga cccaagtgag ttccttcact cagcgccccc acgcccgacc ttcactctcg
     1681 acccgccgct tcttcagccg aagcgcagga ccttgcgctt cttcaccttg cgttgcgcct
     1741 cgggcgacgg tcccggcgcg ggcgccgctg cggccggctc tgacgcaggc gtggcggcag
     1801 gctcggccgc tggtgcctgc gaggaggtac gcgacgtggc cccgccgcct tcctgccggc
     1861 tggcgcgccc cgtccgtccg aacgagcacc ctttgcaggc ccgctcccgg ccgacccgcc
     1921 gtcggcagac ccgctcttgg cagacccgcc cttggcggac ccgttcttcg ctggcgcacc
     1981 cttggcggaa gcgcccttgg tagacccgga cgtggcgacc accccttcgg cggcgccctg
     2041 ctgcggcgtc ttgagcttgc cgggcgggcg ggtgtcggcc tgcttctcct cctcgacctc
     2101 cggcatcggc aggtcggcag gcagcaggtc tatctggcgt tgcgtcgggt tggccgcgag
     2161 gatcttgacc tccaccctgt cgcccatgcg gtagcgcttg cgcgtgtgct tgcccatgag
     2221 catgagctgg tcctcgatgt agaggtagta gtcgtccacc aggtggctga cgtgcatgag
//
LOCUS       DAOQYE010000289         2278 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_53596_length_2278_cov_2.909582, whole genome shotgun sequence.
ACCESSION   DAOQYE010000289 DAOQYE010000000
VERSION     DAOQYE010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53596_length_2278_cov_2.909582"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="PKN52_05580"
     CDS             complement(<1..294)
                     /locus_tag="PKN52_05580"
                     /EC_number="2.3.1.275"
                     /inference="COORDINATES: protein motif:HMM:NF014690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HNQ99449.1"
                     /translation="MIALLAALAGGYLLGAIPTAALIARAKGKDVFTLGSGNMGAMNT
                     ARNLNIWLGALVLLLDVAKGAAATGVGMLVASLTPESATAAATATSAAAAAATA"
     gene            complement(291..1010)
                     /gene="recO"
                     /locus_tag="PKN52_05585"
     CDS             complement(291..1010)
                     /gene="recO"
                     /locus_tag="PKN52_05585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HNQ99450.1"
                     /translation="MRRYTVADGVVIKRHPLPSGDLVVTLLGRMGKWRAVARKGTLPG
                     GNIGRLSLFHDVTVQFYRRNEEDLALLTQVQLNGALPALSGTTTYAYAHLLCELADAL
                     TVDLHLEARVYDYLTSGLRGLASHHDPELVALLYAWRLLGVAGLAPRVGACVACGAVA
                     PLVALDVAAGGLTCDRDRSGLTLTTTEVGELQALVGGGMAEALATELPRRDRHWRLLE
                     AYVAYHVQELRSFASLHAGAA"
     gene            complement(1069..1377)
                     /locus_tag="PKN52_05590"
     CDS             complement(1069..1377)
                     /locus_tag="PKN52_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99451.1"
                     /translation="MSAAGGSLGRGLNLAALARSAGGEPCEVPGWPLEAQSLMVPTAV
                     AGDRARWLVVLEGEVIVDLPSGDFRVLKAGDGLAFPIGVEAVLRSVARPAVLLWHAAA
                     "
     gene            complement(1387..>2278)
                     /locus_tag="PKN52_05595"
     CDS             complement(1387..>2278)
                     /locus_tag="PKN52_05595"
                     /inference="COORDINATES: protein motif:HMM:NF024761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /protein_id="HNQ99452.1"
                     /translation="ATEPGSGTPVYLKRGPFGPYLQLGDAGSDGGKPKRVSLPPGVEP
                     HEVSSDLALRLIALPLRLGDHPEDGKPVDLGIGRYGPYVKHGSTFASIPKGEFVLDVN
                     LERALQLLAQKARRGTAALRVVGADPRTGEAIELYEGRFGPYVKRGSVNASLPRDVAP
                     EALELDQAVALLDAREAAGPSKAKGRRGTGAGAKTTGAKTKRGKAGGKGARKPSGPAA
                     PKAKATPEQLVAYLGELDPADAAVVSLTLGAHGPGLDLAAAAARLGIDRSEAETRNKR
                     ALFKLRMSFGRDRAKQPDGG"
BASE COUNT          305 a          809 c          833 g          331 t
ORIGIN      
        1 cgcggtcgcc gctgcggcgg cggccgatgt ggcggtggct gccgcggtcg cgctctcggg
       61 cgtgaggctg gcgacgagca tgccaacgcc cgtggccgca gcccccttcg ccacgtctag
      121 caaaagcacg agcgcgccga gccagatgtt gaggttgcgc gccgtgttca tggcgcccat
      181 gttccccgag ccgagagtga agacgtcctt gcccttggct cgcgctatca gcgcggccgt
      241 cggtatggcg ccgaggaggt agccgcccgc caacgcggcg aggagggcga tcatgccgcc
      301 cccgcatgca acgacgcgaa gctgcgcaac tcctgtacgt gatacgccac gtaggcctcc
      361 agcagcctcc agtgccggtc tcggcgcggc agctcggtcg cgagcgcttc cgccatgccg
      421 ccgcccacca gcgcttgcaa ctcgccgacc tcggtagtcg tgagggtcag cccgctccgg
      481 tcgcggtcgc aagtcaggcc cccggccgcg acatcgaggg cgacgagtgg agcgacggcc
      541 ccgcacgcca cgcacgctcc cactcgtggg gcgagtccgg cgacgccgag gagccgccaa
      601 gcgtagagca gcgcgaccag ttcggggtcg tggtgcgagg cgagcccccg gagaccggac
      661 gtgaggtagt cgtacacccg cgcctccagg tgcaggtcga ccgtcagggc gtcggcgagc
      721 tcgcatagta ggtgcgcgta cgcgtaggtc gtcgtgccgc tgagggccgg tagggcgccg
      781 ttgagttgga cctgcgtgag gagcgccaag tcctcttcgt tgcgccggta gaactgcacg
      841 gttacgtcgt ggaagaggga gagacgaccg atgttccccc cgggcagcgt gcctttgcga
      901 gcgacggccc gccacttgcc catgcgccct aggagggtga cgaccaggtc tccggacggg
      961 agcgggtgcc gcttgatgac gaccccgtca gcgacggtgt agcgcctcac ccttcgagtc
     1021 tagcgtgctc gcgccgccgc taccgcgacg cgggcaggcg gcccggacct aggcagccgc
     1081 gtgccatagg aggacggccg gacgcgccac gctgcgcagc actgcctcga cgccgatggg
     1141 gaacgcgagg ccgtcgccgg ccttgaggac tctgaagtcg ccggagggca ggtcgacgat
     1201 cacctcgccc tcgagcacca cgagccacct ggcgcggtcc cccgccaccg cggtcggtac
     1261 cattaggctc tgcgcctcga gcggccagcc gggcacctcg cacggctcgc cgccggccga
     1321 ccgggcgagc gcggccaggt tcaggccgcg gccgagggac cctcccgcag cgctcacggc
     1381 ccgctctcaa ccgccgtctg gctgtttggc gcggtcgcgt ccgaagctca tgcgcagctt
     1441 gaacagggcg cgcttgttgc gggtctccgc ctcactgcgg tcgatgccga ggcgcgccgc
     1501 cgcggcggcc aagtcgagac ccggaccgtg cgctccgagc gtgagcgaca ccaccgccgc
     1561 atcggccggg tcgagctcgc cgaggtaagc gacgagctgc tcgggtgtgg ccttggcctt
     1621 cggcgcggcg gggccggagg gcttgcgcgc ccccttgccg ccggctttcc ctcgcttggt
     1681 cttggcacca gtggtcttgg caccagcgcc cgtgcccctg cgtcccttcg ccttcgacgg
     1741 cccggcggcc tcacgcgcat cgaggagcgc cacggcctga tccagttcga gcgcttcggg
     1801 cgccacgtcg cgcggcaggg acgcgttcac gctgccgcgc ttgacgtacg ggccgaagcg
     1861 tccttcgtac agctcgatgg cctcgcccgt gcgcgggtcg gcgccaacga ccctgagcgc
     1921 cgcggtgccg cggcgggcct tctgggcgag gagttgcagc gcgcgctcca ggttcacgtc
     1981 gagcacgaac tcgcccttgg ggatggaggc gaaggtgctc ccatgcttga cgtacgggcc
     2041 gtaccggccg atgccgaggt cgacgggctt gccgtcctcc gggtggtcgc cgaggcgcag
     2101 cgggagcgcg atgagccgca gcgccaggtc gctcgagacc tcgtgcggtt ctacgccggg
     2161 gggtagggag acgcgcttgg gtttgccccc gtccgagccc gcatcgccga gttgcaggta
     2221 ggggccgaac gggcctcgtt tcaagtagac gggtgtgccg gagccgggct cggtcgcg
//
LOCUS       DAOQYE010000290         2273 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_53780_length_2273_cov_2.355726, whole genome shotgun sequence.
ACCESSION   DAOQYE010000290 DAOQYE010000000
VERSION     DAOQYE010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2273
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53780_length_2273_cov_2.355726"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="PKN52_05600"
     CDS             <1..225
                     /locus_tag="PKN52_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003026825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HNQ99453.1"
                     /translation="VRAWTVKAGSKAPQAAGEIHSDFERGFIRAEVVSYEDLVAAGSL
                     ANARARGKLRVEGKEYVVQDGDVMNFLFNV"
     gene            complement(289..>2273)
                     /locus_tag="PKN52_05605"
     CDS             complement(289..>2273)
                     /locus_tag="PKN52_05605"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HNQ99454.1"
                     /translation="DPQQYSTTVEAVATRYRNLGYRGSGVDTSTTSLVGGVFTVRVLE
                     LTIASIDTTALGVDPTALTLHPGDLFNYDKLLSDVKRLARGRSSDIQLEAGVSASGGV
                     RVTFKLGAPETAGTVDAIRIEGNTVLTTEELTAALNLQVGDTFTSVLAQEDFGAIVRA
                     YQAAGYRVVTRPDFSYDDGTYVQRVTELKTVGYELRYQGEPSSTKESVVTRYLPPVGS
                     VVSDKKIVDGLRQVASLGVVDVLNYNLEPGDAGDEARVILTLAKRQTGELRPAAQYAT
                     DTGFSGSLSYSEKNFLGLAHTVGAEVDVLNTDLGIMLGGRLNYSIPWLYVDALDFMEV
                     PTAINLSLFSVVSNNNPLTVGNRTTVTYPGLAEREENQVRVGDYSLRSSGLGFSVGRP
                     IADSTYLTVSANGAYNEYKLEPPTAECEVQGGVVQNPKTCSTPSGFAINYLPTSGLSA
                     FVGGRVGYDTRTDTNFPADGMGAYGSLGVGFGNDFLLNGARASYVYEQVTAGVRAYAR
                     LSDLLPAEVTDKNHVFAVRLDVGHQFGGEYPVSKRFTVGRTNELSTQIRGYTMDDFSL
                     SKSYATASFEYRYDFGLSTVATQTVIGLAFVDLGWASSAPGFADYETPVFAGAGLGIQ
                     VNLGFAGIVLPAIRLDYAFSERHPTGVFAFRVGPVF"
BASE COUNT          352 a          727 c          835 g          359 t
ORIGIN      
        1 gtgcgcgcct ggaccgtcaa ggccggcagc aaggcgcccc aggcggcggg cgagatccac
       61 tccgacttcg agcgcggctt catccgtgcc gaggtcgtga gctacgagga cctcgtggcg
      121 gccggcagcc tggccaacgc ccgcgcacgc ggcaagctcc gcgtcgaggg caaggagtac
      181 gtcgtccagg acggcgacgt gatgaacttc ctcttcaacg tctagcggct acctagtcgc
      241 ccggtcggcc gacgggcccg tcaacgggcc ccctcgcccc gagccgccct agaacacggg
      301 ccccacgcgg aacgcgaaca cgcccgtcgg gtgcctctcg ctgaacgcgt agtcgagccg
      361 gatggccggc agcacgatgc cagcgaagcc taagttcacc tggatgccaa ggcccgcgcc
      421 ggcgaagacg ggcgtctcgt agtcggcgaa gccgggcgcg ctcgacgccc aaccgaggtc
      481 gacgaacgcc agcccgatga ccgtctgggt ggcgacggtc gagaggccga aatcgtaacg
      541 gtactcgaac gaggccgtgg cgtagctctt cgagagcgag aagtcgtcca tggtgtagcc
      601 gcggatctgg gtggagagct cgttcgtgcg gccgaccgtg aagcgcttcg agacggggta
      661 ctcgccgccg aactggtggc cgacgtcgag cctgacggcg aagacgtggt tcttgtccgt
      721 gacctccgcc ggcagtaggt cggacaggcg cgcgtaggcg cgcacgcccg ccgtcacctg
      781 ctcgtacacg tagctggcgc gcgcgccgtt caggaggaag tcgttgccga agccgacgcc
      841 gaggctgccg tatgcgccca tgccgtcggc cgggaagttc gtgtcggtgc gcgtgtcgta
      901 ccccacgcgc ccgcccacga aggcggagag accgctcgtc ggcaggtagt tgatggcgaa
      961 gccgctgggc gtcgagcacg tcttcgggtt ctgcacgacg ccgccctgca cctcgcactc
     1021 ggcggtaggc ggctcgagct tgtactcgtt gtacgcgccg ttcgccgaca cggtgaggta
     1081 ggtggagtcg gcgatgggcc ggccgacgga gaagccgagg ccgctggagc gcagcgagta
     1141 gtcgcccacg cgcacttggt tctcctcgcg ctccgcgagt cccggatagg tgacggtggt
     1201 gcggttgccg accgtgaggg ggttgttgtt gctcacgacg ctgaagagcg agaggttgat
     1261 ggcggtcggc acttccatga aatcgagcgc gtcgacgtac agccagggga tggagtagtt
     1321 caggcgcccg ccgagcatga tgcccaggtc ggtgttgagg acgtccacct ccgctccgac
     1381 ggtgtgggcg agaccgagga agttcttctc cgagtacgag aggctgccgg agaagcccgt
     1441 gtccgtcgcg tactgcgcgg ccggccgcag ctcgcccgtc tggcgcttgg ccagcgtgag
     1501 gatgacgcgc gcctcgtcgc ccgcgtcgcc aggctccagg ttgtagttga ggacgtcgac
     1561 cactccgagc gaggcgacct gcctgaggcc gtcgacgatc ttcttgtcgg agacgacgct
     1621 gccgacgggc ggcaggtagc gcgtcacgac gctctccttg gtggagctcg gttcgccctg
     1681 gtagcggagc tcgtacccga cggtcttgag ctccgtgacg cgctggacgt acgtgccgtc
     1741 gtcgtaggag aagtcgggcc gcgtgacgac gcggtagccg gccgcctggt aagcccggac
     1801 gatggcgccg aagtcctcct gggccagcac ggaggtgaac gtgtcgccga cctgcaggtt
     1861 gagggcggcg gtgagctcct cggtggtgag gacggtgttc ccctcgatgc ggatggcgtc
     1921 gacggtgccc gcggtctccg gagcgccgag tttgaacgtc actcgcaccc caccgctcgc
     1981 cgacacgccc gcctccagct ggatatcgga ggagcggccc cgggccaagc gcttaacgtc
     2041 ggagaggagc ttgtcgtagt tgaacaggtc gccggggtgc agggtcaacg ccgtcgggtc
     2101 gacgccgagc gccgtcgtgt cgatggacgc gatggtcaac tccaagacgc gcacggtgaa
     2161 gacgccgccg acgaggctgg tggtgctcgt gtcgacgccg ctaccgcggt agccgaggtt
     2221 gcggtaacgc gtggcgaccg cctcgacggt ggtgctgtac tgctgggggt cga
//
LOCUS       DAOQYE010000291         2272 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_53819_length_2272_cov_2.407307, whole genome shotgun sequence.
ACCESSION   DAOQYE010000291 DAOQYE010000000
VERSION     DAOQYE010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53819_length_2272_cov_2.407307"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="PKN52_05610"
     CDS             complement(<1..306)
                     /locus_tag="PKN52_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNQ99455.1"
                     /translation="MSSANRLLEVNNLKVYFDTDNGTVKAVDDISFHIDKGETLAVVG
                     ESGSGKSVTSLAVMRLLPTPPARFAGGEIKFAGRDIMKFSEREMRKIRGNDISMIFQE
                     "
     gene            complement(343..759)
                     /locus_tag="PKN52_05615"
     CDS             complement(343..759)
                     /locus_tag="PKN52_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99456.1"
                     /translation="MLDPESNTGLQTDVASSSACGPVTIVASLGKDRFVARCTCGYLH
                     LVWDNASLSLRLRDLQQLLASPLRQEPSVAAQFELRLDPAGGIQLWAGTGGIRVNAVA
                     RAALEGMLRTAVDSCFALPGAQEDAVKPLDTSNLLN"
     gene            complement(975..1553)
                     /locus_tag="PKN52_05620"
     CDS             complement(975..1553)
                     /locus_tag="PKN52_05620"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNQ99457.1"
                     /translation="MDLPLGAFEVAIYPATGRFDDFAAVVGPKTGKQGCWCMAYRNSS
                     LDADGRIAHMRAECATEPGPGVLAYVDGEAAGWCSVAPRSTYRRLMNSRTIPFVDDRD
                     AWSVVCFVVRAGYRRQGLMHYLLEGAVEHARERGGEVVEGYPADAGEGRIDLISGYVG
                     TTRLFEAHGFTRAIQTAGVSGGRPRWVMRREL"
     gene            complement(1556..2035)
                     /locus_tag="PKN52_05625"
     CDS             complement(1556..2035)
                     /locus_tag="PKN52_05625"
                     /inference="COORDINATES: protein motif:HMM:NF037170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNQ99458.1"
                     /translation="MSKVVRHEVRDFPAARDLPRPVSPEPASEASQARSVLFILYVRD
                     QAASAAFYRAVLKRTPSLDVPGMTEFEVGAQARLGLMPEAGIRRLLGEAVPDPGRASG
                     IPRSEVYLVVRDPSEYHRRAIEAGAAELSPVAPRGWGDDVGYSLDPDGHVLAFARPS"
     gene            complement(2019..>2272)
                     /locus_tag="PKN52_05630"
     CDS             complement(2019..>2272)
                     /locus_tag="PKN52_05630"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HNQ99459.1"
                     /translation="SSRMEAKDGSTGFDFAGTYTTVVEGKLIEYTFGDREARVEFGEV
                     PGGVRVRVTFDAEDQNPIELQRGGWQAILDSFARHVEGR"
BASE COUNT          364 a          765 c          764 g          379 t
ORIGIN      
        1 ctcctggaag atcatcgaga tgtcgttgcc gcggatcttg cgcatctcgc gctcgctgaa
       61 cttcatgatg tcgcggccgg cgaacttgat ctcgccgccg gcgaagcgcg caggcggcgt
      121 cgggagcagg cgcatgacgg ccagcgaggt cacggacttg ccggagccgg actcaccgac
      181 cacggccagg gtctcgccct tgtcgatgtg gaacgagatg tcgtcgaccg ccttgacggt
      241 gccgttgtcg gtgtcgaagt agaccttgag gttgttgact tccagcaagc ggttcgcgct
      301 tgacatcggt ttcacgctcc ttgggctcgc tacggcgagt agtcagttga gcaggttcga
      361 ggtgtcgagg ggcttgacgg cgtcttcctg cgcgcccggc aaggcgaagc aactgtcgac
      421 cgccgtgcgc agcatcccct ccagcgcggc tctggcgacc gcgttcacgc ggatcccgcc
      481 ggtgcccgcc cacagctgga tgccgccggc cgggtcgaga cgcagctcga actgggccgc
      541 cacgctcggc tcttgccgca gcgggctggc gagcaactgc tgcaggtcgc gcagtcggag
      601 gctcaaagag gcgttgtccc agacgaggtg caggtagccg cacgtgcagc gggccacgaa
      661 cctgtccttg cctaggctgg cgacgatggt aacgggaccg caggccgagc ttgaggctac
      721 gtcggtctgc agcccggtat tcgactcggg atcgagcatc tcatccacct tcactctggc
      781 ttgtccccac cctggacaat ccatagtaaa gcactatgaa tagaattcat agtcagcagc
      841 tatggattga ggtggtgggg tgcgggtggg aaggatgaac ctaggaccgt ccgaccgaag
      901 tcccgcgagc ctgcaccgta gcggcgttct cgagagtctg ttcccggccg caaggcgtgc
      961 gattcgaggg gctgttagag ctcccgccgc atgacccatc gtgggcgccc gccgctcacg
     1021 cccgccgtct ggatcgccct ggtgaagccg tgggcctcga acaggcgggt agtgccgaca
     1081 tagcccgaga tgaggtcgat gcgaccttcg ccggcgtcgg ccgggtaacc ttcgacgacc
     1141 tcgccgcccc gctcgcgcgc gtgttcgacg gccccttcga gcaggtagtg catcagccct
     1201 tgtctgcgat acccggcgcg cacgacgaag cagacgaccg accaggcgtc gcggtcgtcg
     1261 acgaacggga tggtccgtga gttcatcagc cgacgatagg tcgagcgcgg cgcgacagag
     1321 caccagcccg cggcttcgcc gtccacgtag gcgagcacgc cgggacccgg ttcggtcgcg
     1381 cattcagcac gcatgtgcgc gatgcgcccg tcggcgtcga ggctggagtt gcggtaggcc
     1441 atgcaccagc agccctgctt acccgtcttc ggcccgacca cggccgcgaa gtcgtcgaac
     1501 cgccccgtgg ccgggtagat cgctacttcg aatgctccga gggggaggtc catcgctacg
     1561 agggccttgc gaaggcgagg acatgaccat ccggatcgag cgagtacccg acgtcgtcgc
     1621 cccaaccacg aggtgccacc ggactgagct cggcggcgcc cgcctcgatg gcgcgccggt
     1681 ggtactcgga cgggtcgcgt acgaccaggt agacctcgct cctggggatc ccgctcgcgc
     1741 ggccaggatc cggaacggct tcgcccagga gccggcggat ccctgcctcg ggcatcagcc
     1801 cgagcctggc ctgcgctccg acctcgaact cggtcatgcc cggcacgtcc aagctgggcg
     1861 ttcgcttgag gacggcgcgg tagaacgcgg cgctggccgc ttggtccctc acgtacagga
     1921 tgaacagtac tgaccgcgcc tgagatgcct ccgaggcggg ctcggggctg acaggcctgg
     1981 gcaggtcgcg ggccgcgggg aagtcccgca cctcgtgcct aacgaccttc gacatggcgc
     2041 gcgaagctgt cgaggatcgc ctgccagccg ccacgctgca actcgatggg gttctggtcc
     2101 tcggcgtcga acgtgaccct caccctgacg ccgcccggca cctcaccgaa ctccacccgc
     2161 gcctcgcggt cgccgaacgt gtactcgatc agcttgccct ccaccaccgt ggtgtaggtg
     2221 ccggcgaagt cgaagcccgt gctgccgtcc ttcgcttcca tgcgggacga ga
//
LOCUS       DAOQYE010000292         2270 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_53870_length_2270_cov_3.260497, whole genome shotgun sequence.
ACCESSION   DAOQYE010000292 DAOQYE010000000
VERSION     DAOQYE010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53870_length_2270_cov_3.260497"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(78..1148)
                     /locus_tag="PKN52_05635"
     CDS             complement(78..1148)
                     /locus_tag="PKN52_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99460.1"
                     /translation="MDEIARVEQMVAQGLISADDGERLKEVLRSVQEAEQEGTAPQLA
                     AAGDDRPALPYDASVVSPAGPATLGGPAATPSAPGLSAPIPPVPPLAPTASPAAAATA
                     APNAAPNAATNATANAAPSGAQASTDRPAPAADAGLPAGTKWVRVELVAGDLDVGVDP
                     QLTEPRAESDTGEAALERTDYGYRVSIGRGEGSFLRGLEGPLAGLLISKGRPGDLKLT
                     LPAGYGVDLAMTTGDVDLDGVPFLRGTLSAGDLDAHGLEGINFSSMAGDVSVSLRPTS
                     GRHRIVNTAGELKVTLLPGSDVAVHGDVSIGEAKAGPEFQTGRRMMSGTVSGAIGAGT
                     ARLDLKVTAGSIKLRTGTDDRG"
     gene            complement(1212..1511)
                     /locus_tag="PKN52_05640"
     CDS             complement(1212..1511)
                     /locus_tag="PKN52_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99461.1"
                     /translation="MNLDLSSCQGGEMMNAYFDQRHSLDHVSQLQQEAATDRLVRSHG
                     TPRPGARLRGRFAQWLRNAAARIDSGEDRQESMGRLGRLDAGMRDDRVEALPYSH"
     gene            complement(1530..>2270)
                     /locus_tag="PKN52_05645"
     CDS             complement(1530..>2270)
                     /locus_tag="PKN52_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99462.1"
                     /translation="LGRGLADRVGAGEPAEVAAEAVLAARLRAHRVQAGDEEREPRGR
                     GVGQALKWQQRDWCLKRDLAWVTWTFDPLQARNANLNLRHLGAICVDYLVDFYGPMGG
                     PLGGARSDRFLALWVLTAPSVVAIANGGAGGRPTPGAATVEPTATRGSAVWAVRPPID
                     GGGAEPVVELAGPGLMSVAEAGARVMVAAPPTDRDADPARARRWQDAFAQTIVPLFEA
                     GYVAVDLLEGAYVLEPPGGGFKAKLDNL"
BASE COUNT          301 a          785 c          808 g          376 t
ORIGIN      
        1 ccgccggccg catcacggct gccgccgcga tgcggccggc ggcaagcatg tcgaggatgc
       61 gcttgcgggt gtccgaatca gccacggtcg tccgttcccg tcctgagctt gatgctcccg
      121 gccgtgacct tgaggtcgag cctggcggtc ccggccccga tggcgccgct gaccgtcccg
      181 ctcatcatcc gcctgccggt ctggaactcc ggacccgctt tcgcctcgcc tatggagacg
      241 tcgccgtgga cggccacgtc cgaaccgggc aggagcgtga ccttcagctc gcccgcggtg
      301 ttgacgattc ggtgtctgcc gctcgtcggc cgcaagctca cggacacgtc ccccgccatg
      361 ctggagaagt tgatgccctc cagaccgtgg gcatcgaggt ccccggccga gagggtgccg
      421 cgcaggaaag gcacgccgtc gaggtcgacg tcgcccgtcg tcatcgcaag gtcgacgccg
      481 tagccggccg gcagggtgag cttgaggtcg cccggtcggc ctttgcttat gagcaacccg
      541 gcgagcggac cctcgaggcc gcgcaggaaa gatccctcgc ccctcccgat ggagacgcgg
      601 tagccgtaat cggtgcgttc gagcgcggcc tcccccgtgt cggactcggc tcgcggctcg
      661 gtgagttgcg ggtcgacgcc gacgtcgagg tcgcccgcca ccagctcaac gcgcacccac
      721 ttcgtgccgg ccggcagacc tgcgtcggcg gccggtgcag gcctatcggt cgacgcctgc
      781 gcaccggacg gtgcggcgtt tgccgtggcg tttgtagcgg cgtttggtgc ggcgtttggt
      841 gcggcggtcg ccgcggcggc tggcgaagcg gtcggcgcca gcgggggaac gggcgggatc
      901 ggcgcggaaa gtcctggcgc ggacggcgtt gccgcaggtc ctccgagcgt tgccggcccg
      961 gccgggctca ctaccgaagc gtcgtacggc agggccggtc ggtcgtcacc cgctgcagcc
     1021 agctgcggcg cggtgccttc ctgttctgcc tcctgaacgg agcgcagcac ttccttgagc
     1081 cgttcgccat catcggcgct gatcaggccc tgagccacca tctgttctac gcgggcgatc
     1141 tcgtccatcg ttgctcctgt tcgtgtccgg gtgtctcgct gcctcgcccg tgctggcgag
     1201 gcgcctgctg gtcagtgtga atagggaagc gcttcgacgc ggtcatcccg catgccggcg
     1261 tccagcctgc ccaggcggcc catcgactcc tggcggtctt cgccggaatc gatgcgggcc
     1321 gccgcgttgc gaagccactg ggcgaagcgt ccgcgcaggc gtgcgccggg acgcggcgtg
     1381 ccgtgggagc gcacgaggcg gtcggtcgcg gcctcttgct gaagttgaga aacgtggtct
     1441 agcgagtggc gttgatcgaa gtatgcgttc atcatctcgc ctccttggca agatgatagg
     1501 tcgaggttca tagaatgtca agtgtggatt tatagattgt caagtttagc tttgaaccct
     1561 ccgccgggtg gctccaggac ataggcgcct tcgagtaggt cgacggccac gtagcccgcc
     1621 tcgaacagcg gcacgatggt ctgggcgaac gcgtcctgcc agcgtctggc gcgcgccggg
     1681 tcggcatcgc ggtcggtcgg tggcgcggcc accatcacgc gcgcgccggc ttcggcgacg
     1741 ctcatcaggc cgggcccggc gagctccacc acgggctcgg cgccgccacc atcgatcggg
     1801 ggccgaacgg cccagaccgc ggagccccgc gtcgccgtcg gctcgacggt ggcggcgccg
     1861 ggcgtgggtc ggccaccggc cccgccgttg gcgatcgcga cgacgctcgg cgccgtgagc
     1921 acccagagcg cgaggaacct gtcgctcctc gcgccaccga gcgggccgcc catgggcccg
     1981 tagaagtcga cgaggtagtc gacgcagatg gccccgaggt gacgcaagtt caggttcgcg
     2041 ttgcgtgcct gcagtggatc gaacgtccat gtgacccaag cgaggtcacg cttgaggcac
     2101 cagtcgcgct gctgccactt gagcgcctgc ccgacgcctc gaccgcgcgg ttctcgttct
     2161 tcgtcaccag cctggacacg atgcgcgcgc agtcgggcag ccaggacggc ttcggcggcg
     2221 acctcggcgg gctcgccggc gccgacgcga tctgccagac cgcggccgag
//
LOCUS       DAOQYE010000293         2270 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_53875_length_2270_cov_2.797743, whole genome shotgun sequence.
ACCESSION   DAOQYE010000293 DAOQYE010000000
VERSION     DAOQYE010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53875_length_2270_cov_2.797743"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="PKN52_05650"
     CDS             complement(<1..185)
                     /locus_tag="PKN52_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99463.1"
                     /translation="MATYTELHALRGAASTEPLKQKIAVAIAIKANAIAKLQAAEAPQ
                     KAWALAALANPGKDVDT"
     gene            complement(188..649)
                     /locus_tag="PKN52_05655"
     CDS             complement(188..649)
                     /locus_tag="PKN52_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99464.1"
                     /translation="MANIVFNIAKGRVVEYYNRVKSNDPANSALILVPIETSGLESDA
                     TLIDVDTLAALLAGATNEQTTMGRKTLTDSDLAALPSPDDANDRYEVSLPTVTWAAAS
                     GNAISKIAVCYDSDTTSGTDSNIIPLTMFDFAQTPSGADIQMTTGVFFRAS"
     gene            complement(653..1669)
                     /locus_tag="PKN52_05660"
     CDS             complement(653..1669)
                     /locus_tag="PKN52_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99465.1"
                     /translation="MGDTLATLVEEVQYELDQVVSPAAGQNFREHIKNRIRREYRRLY
                     HDFDWPDLRQWTDRGTSAGQRYYNWPTGLSLATVSDVFILWGDRYHKLERGIDPCHYN
                     EYNSDQGARMDPTLRWSPYGQAQLEIWPIPASASSLRFVGRRAFTPLVDEDDRCDLDT
                     DLVVLFAAAKLAKTEDRKVQLLAEAQAHYKTLKQRLTRGNIRINFAKDYSEDPRHFGA
                     PMPYVAVAEASVSGTATYAVFFPDFPGASEFINVDVPLSFTAGIDYSSASAEQAATDA
                     AVFTITADGEAVGTVTFGPGETEGVFAFTNTTVPEDASLKFAAPATPDATLQDISITL
                     GGTR"
     gene            complement(1679..2119)
                     /locus_tag="PKN52_05665"
     CDS             complement(1679..2119)
                     /locus_tag="PKN52_05665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99466.1"
                     /translation="MRQYSCSVRLAGEMLQVVTGKIVTVPEIAVLRDVHGADAVLDIR
                     PADPVERDDATERERLRKRYDNPTPDSQPRVDRLFGPAFQPLPKTLREIGLDPASQAA
                     VLRAQAEAAATAAALLENEPSDDTGDVDEMFGDDAPASKKAKAA"
BASE COUNT          331 a          693 c          825 g          421 t
ORIGIN      
        1 atcgtgtcga catcctttcc ggggtttgcc agcgcggcca atgcccatgc cttttgcgga
       61 gcctcggcgg cctgcagctt ggcgatggcg tttgccttga tggcgatggc gaccgcgatc
      121 ttttgcttga gcggttcggt cgatgcagcg ccgcgcaggg cgtggagttc ggtgtatgtt
      181 gccatgatta gctcgcccgg aagaacacgc cagtcgtcat ctgaatgtca gcaccgctgg
      241 gcgtctgcgc gaagtcgaac atggtcagcg ggatgatgtt gctgtccgtc ccgctcgtgg
      301 tgtcgctgtc gtagcagacc gcgatcttgc tgatcgcgtt gccggaggcc gccgcccagg
      361 tgacggtggg caggctcacc tcgtagcggt cgttggcgtc gtcgggcgac ggcagggcgg
      421 ccaggtccga gtcggtgagc gtcttccgac ccatggtcgt ctgctcgttc gtggccccag
      481 ccagcagcgc cgccagggtg tccacgtcga tgagcgtggc atccgactcc agccccgatg
      541 tctcgatggg gacgaggatc agcgccgagt tcgccggatc gttgctcttg acccggttgt
      601 agtactcaac gacgcgaccc ttcgcgatgt tgaagacgat gttggccata ggttacctcg
      661 tgccgccaag ggtgatggag atgtcctgga gcgtcgcgtc tggcgtcgcc ggcgcggcga
      721 acttgagcga cgcgtcttcc gggacagtcg tgttggtgaa ggcgaagacg ccctcggtct
      781 cgccgggccc gaaggtgact gtgccgacgg cctcgccgtc cgccgtgatc gtgaacaccg
      841 ccgcgtcggt ggcagcctgc tccgccgacg cgctggagta gtcgatcccc gcggtgaagg
      901 atagcggcac atccacgttg atgaactcgg aggcgcccgg gaagtcgggg aagaacacgg
      961 cgtaggtcgc ggtgccggag acgctcgcct cggcgacggc cacgtaaggc atcggggctc
     1021 cgaagtggcg cggatcctcg ctgtagtcct tggcgaagtt gatccggata ttgccgcgcg
     1081 tcaggcgctg cttgagcgtc ttgtagtggg cctgcgcctc ggcgagcagt tggaccttgc
     1141 ggtcctcggt cttcgccagc ttggcggccg cgaacaggac gaccaggtcg gtatccaagt
     1201 cgcagcggtc gtcctcatcg accagcggcg tgaaggccct gcggcccacg aagcgcagcg
     1261 agctcgccga cgccggaatg ggccatatct caagctgggc ctggccgtag ggcgaccagc
     1321 gcagggtggg gtccatccgc gcaccctggt cgctgttgta ctcgttgtag tggcaggggt
     1381 cgatgccgcg ctccagcttg tggtagcggt cgccccagag gatgaagacg tcgctgacgg
     1441 tagcgagcga caggcccgtg ggccagttgt agtagcgctg gccggccgag gtcccgcggt
     1501 cagtccactg cctcagatcc ggccagtcga agtcgtggta gagccgccgg tactcacgcc
     1561 ggatgcggtt ctttatgtgc tcgcggaagt tctggccggc agcagggctg acgacctggt
     1621 ccagctcata ctggacttcc tcgacaaggg tcgcgagcgt gtcacccacc gatcagccct
     1681 aggcggcttt cgccttcttg gacgccgggg cgtcgtcgcc gaacatctcg tcgacatcgc
     1741 cggtgtcgtc actcggctca ttctcgagga gcgccgcagc cgtcgcggcc gcttcagctt
     1801 gggcgcgcag tacggcggcc tggctggcgg ggtcgaggcc gatctcgcgc agggtcttgg
     1861 ggagcggctg gaaggccggc ccgaaaaggc gatcgacgcg cggctgggag tcgggcgttg
     1921 gattgtcgta ccgcttgcgc aggcgctcgc gctcggtcgc gtcatctcgc tcgaccgggt
     1981 cggcgggacg gatgtccagc accgcgtcgg cgccatggac gtcgcgcagg accgcgatct
     2041 cgggaacggt gacgatcttg ccggtgacga cttggagcat ctcgccggcg aggcggacag
     2101 agcaggagta ttggcgcatg gggttcctga agaagcggcc cccggggttg ccgggggcct
     2161 gttgcggagg gactagcccg cgtactgcga cacgccgagg aaggtcgggt cggccagaac
     2221 gaggtcgagc tcgaacacct tggcgccgtc gcaggcagcg ttcgggtcgt
//
LOCUS       DAOQYE010000294         2265 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_54060_length_2265_cov_2.685068, whole genome shotgun sequence.
ACCESSION   DAOQYE010000294 DAOQYE010000000
VERSION     DAOQYE010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54060_length_2265_cov_2.685068"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            126..1208
                     /gene="rfbB"
                     /locus_tag="PKN52_05670"
     CDS             126..1208
                     /gene="rfbB"
                     /locus_tag="PKN52_05670"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: protein motif:HMM:TIGR01181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HNQ99467.1"
                     /translation="MTTYLVTGAAGFIGSALVRQLVARGGTDGARVVSVDAFTYAADP
                     ERLRPAGGEPAHRLVRLDINDTAGMALLLEEERPEVVFHLAAETHVDRSIDGPGAFLR
                     ANTIGTFNLLEAVRRYWQALPAAERAAFRLVNVSTDEVYGSLGPTGVFDEGSPYDPRS
                     PYSASKAGADHFASAYFHTFGLPVMTTHAANNYGPFQFPEKLVPLAINRALAGESVPL
                     YGDGMQVRDWLHVDDHARGLVVVAERGRPGATYLMGGGHELPNRALLELLLGHLNELA
                     PGQGDYRLLIRSVADRPGHDRRYAVDSSRAENELGWRRLVPFDEGLRDTVKWYVDNRE
                     WVRSVSERYDLGRLGLGVGMAGAPSA"
     gene            1205..2077
                     /gene="rfbA"
                     /locus_tag="PKN52_05675"
     CDS             1205..2077
                     /gene="rfbA"
                     /locus_tag="PKN52_05675"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HNQ99468.1"
                     /translation="MNRRGIVLAGGSGTRLYPATLAVSKQLLPVYDKPMVYYPLTTLM
                     LAGIREVLLISTPRDLPAFHALLGTGEEWGMHFEFAVQERPEGIAQALVIGSEFLADR
                     PSALVLGDNLFHGNGLTDLLAVAGSRTDSATVFGYQVTDPQRYGVVAFDEHGRATSIE
                     EKPARPRSRYAVTGLYFYPAGAADEARLLSPSKRGELEITDLNLSYLRQDRLRVELLG
                     QGMAWLDTGTHESYLQASNYVETLEARQGVKIGCPEEVALRNGWIGTGQVARLAEAMR
                     GSGYGRYLLELLER"
BASE COUNT          362 a          761 c          790 g          352 t
ORIGIN      
        1 gcgggtctgc gctgggacga cccgaccgtc ggggtggact gggggatcgt cgatccgatc
       61 ctgtccgcaa gagacgccca gctgccttcg ctcgccgacc tgaaggcgtt accggaaggc
      121 cgaccatgac cacctacctg gtgaccggcg cggcgggctt catcggctcc gccctggtgc
      181 gccagctcgt ggcgcgcggt gggacagacg gtgcgcgagt cgtcagcgtc gacgccttca
      241 cgtacgctgc cgaccctgag cgcctacggc ccgcgggggg tgagccggcg catcgactcg
      301 tgcggctcga catcaacgac acggccggca tggcgctcct cctcgaggag gagcgcccag
      361 aggtcgtctt ccacttggcc gccgagacgc acgtcgaccg ctcgatagac ggtccggggg
      421 ccttcctgcg ggccaacacg atcggcacgt tcaacctgtt ggaggccgtc aggcgctact
      481 ggcaggccct tccggcggcc gagcgcgccg cgttccggct ggtcaacgtg agtaccgacg
      541 aggtctacgg tagcctcggt cccactggcg tgttcgatga gggcagtccg tacgaccccc
      601 gctcgccgta cagcgccagc aaggcgggcg ccgatcactt cgcgagcgcc tacttccaca
      661 cgttcgggct gcccgtcatg acgacgcacg ccgccaacaa ctatggaccg ttccagttcc
      721 ctgagaagct cgtgccgctg gccatcaacc gcgcgctggc gggcgagagc gtgccgctgt
      781 acggcgacgg catgcaggtc cgcgactggc tgcatgtcga cgatcacgct cgcggcctcg
      841 tggtcgtggc ggagcgcggt cggccgggcg ccacctacct catgggcgga ggccacgagc
      901 taccgaaccg cgccctcctg gagctgctcc tcggccacct caacgagctg gcgccagggc
      961 aaggcgacta ccgcctcttg atcaggagcg tcgcggatcg acccggccac gaccggcgct
     1021 acgctgtcga ctcgagccgc gccgagaacg agcttgggtg gcgaaggctc gtgccgttcg
     1081 acgagggcct gcgagacacg gtgaagtggt acgtggacaa ccgggagtgg gtgcgctccg
     1141 tttccgagcg ttacgacctc gggcgcctgg ggttgggcgt tggcatggca ggggcgccat
     1201 cggcatgaac cgccgcggca tcgtgctcgc gggcggttcc ggtacgcgcc tataccccgc
     1261 cacactggcg gtgagcaagc agctccttcc cgtctacgac aagccgatgg tctactaccc
     1321 gctcacgacg ctgatgctgg ccgggatccg cgaggtcctc ctgatctcga cgccgcgcga
     1381 cctaccggcg ttccacgcgc tcctcggcac gggcgaggag tggggcatgc acttcgagtt
     1441 cgcagtgcag gagcgcccgg aagggatcgc gcaggcgctc gtcataggtt ctgagttcct
     1501 ggccgaccgc ccctccgcgc tcgtcctcgg cgacaacctc ttccacggca acggcctgac
     1561 cgacctgctg gccgttgctg gctcccggac cgactccgcc accgtcttcg gctaccaggt
     1621 gacggacccg cagcgctacg gcgtggtggc gttcgacgag catggccgcg ccaccagcat
     1681 cgaggagaag ccggccagac cgcgcagccg gtacgcggtg accggcctct acttctatcc
     1741 ggccggggcg gcggacgagg cgcggctact gagcccatcg aaacgcggcg agctcgagat
     1801 cacggatctc aacctctctt acctgcggca agaccgcctg cgcgtggagt tgctcggcca
     1861 gggcatggcg tggctggaca cgggcacgca tgagagctac ctgcaggcct ccaactacgt
     1921 agagacgttg gaggcccggc agggggtgaa gatcggttgt ccggaggagg tcgcgctcag
     1981 gaacggttgg ataggcaccg ggcaggtcgc gcgcctcgcc gaggcgatgc gcgggagcgg
     2041 gtacgggcgg tacctgttgg agttgctgga gcggtaggtg atctgtgacc agcgggccac
     2101 cagccgcgca aggcgccggg cgggctcaag ggcttggcat gatcgcggtg gcgagcccgc
     2161 tcgcggagcg atagcgcaaa cgtgatatca cgatgagcgc aaatgcgata acctgccgag
     2221 cgcaaacgtg atatcgaaat tcacgcaaac gtgatatctt gtggc
//
LOCUS       DAOQYE010000295         2265 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_54065_length_2265_cov_2.386878, whole genome shotgun sequence.
ACCESSION   DAOQYE010000295 DAOQYE010000000
VERSION     DAOQYE010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54065_length_2265_cov_2.386878"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(89..1189)
                     /locus_tag="PKN52_05680"
     CDS             complement(89..1189)
                     /locus_tag="PKN52_05680"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HNQ99469.1"
                     /translation="MSVPTLDLKGSPFDFGRIHGEAFRSAIRAYAAERVALAGGAAWT
                     GRELSRRDVLALADACLDAHRAYAPDLVTELEGMAKATDLSLAELVVVGGFTDFIDAV
                     AATSAGGGVGLETAAVDDCTAFLVPGSRMADGAAAFGQTWDMHEDSTEHVVLLRGRPK
                     DAPDFLAFTTAGALAMIGMNEAGLSVGINNLMSADGRLGVTWPFAVREMLKAEDLAGA
                     LAALARAPLAGGHNYLVLDASGAGANVEAMPTASHVVRLGERAVAHTNHNLDPSTKAL
                     ERPREAGSQASSEARLAEADRLLDRHDLTVEDLQDVTRHTGTICYRGSAPKFVGTCGG
                     VVTRPRTRELWAVKGLPSEGAYERFAFEATSA"
     gene            complement(1251..>2265)
                     /locus_tag="PKN52_05685"
     CDS             complement(1251..>2265)
                     /locus_tag="PKN52_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ99470.1"
                     /translation="EFFALLGPSGCGKTTTLRVIAGFEEPDSGELLISGRSMRGIPSF
                     HRPVNTVFQDYALFPHMTVLQNVMFGMRMARVPAAEARARAGEALEMVRLPNVERRKP
                     LQLSGGQRQRVALARALVNRPSVLLLDEPLGALDLKLRRAMQFELKRLQQQVGITFVF
                     VTHDQEEALTMADRIAVMNMGEVLQVGRPEDIYERPSGRFVADFIGETNFLHGVLEAK
                     SGSEGSVKLKDGTVLRGVVRDQEIVVGDGVALALRPEKLMVENVEADAPPDLRPGQSA
                     LPGRVSGATYLGTDARYQVEVAGGVTVVARVQNQLHGFDGMLAEGTPVRLSWATEHGS
                     VLA"
BASE COUNT          317 a          829 c          788 g          331 t
ORIGIN      
        1 ccttcagcct gccggccctc cgagtgccgc ccgtccgacc gccgctcgcc cgactgccgg
       61 ccctcagccg gctggccctc cgtcgcgctc atgccgaagt cgcctcgaac gcgaagcgtt
      121 cataggcgcc ctcactcggc aagcccttca ctgcccaaag ttcgcgcgtc cgcggccgcg
      181 tgaccacgcc gccgcacgtg ccgacgaact tgggcgcgga gccccggtag cagatggtgc
      241 ccgtgtgcct ggtaacgtcc tgaaggtcct ccacggtcag atcgtggcgg tcgaggagcc
      301 tgtcggcttc cgccaggcgc gcctcggaac tggcctgcga gccggcctcg cgcgggcgct
      361 cgagggcctt ggtgctcggg tcaaggttgt ggttcgtgtg ggccacggcc cgctcgccga
      421 ggcgcaccac gtggctggcc gtcggcatcg cctcgacgtt ggcgccggcg ccgctggcgt
      481 ccagcacgag gtagttgtgt ccgccggcca ggggggcacg cgcgagggcg gcgagcgctc
      541 ctgcgaggtc ctcggccttg agcatctcgc gcacggcgaa cggccacgtg acgcccagcc
      601 gtccgtcggc gctcatcagg ttgttgatgc ccaccgagag gccggcctcg ttcatgccga
      661 tcatggcaag cgccccggcc gtcgtgaacg ccaggaagtc cggagcgtcc ttcggcctgc
      721 cccgcaggag gacgacatgc tccgtcgagt cctcgtgcat gtcccacgtc tgaccgaacg
      781 ccgccgctcc gtccgccatg cgcgagccgg gtacgaggaa cgcggtgcag tcgtcgacgg
      841 ccgcggtctc cagcccaact ccgcctccgg cgctcgtggc cgccacggcg tcgatgaagt
      901 ccgtgaagcc gccgacgacg accagctcgg cgagcgagag gtctgtcgcc ttcgccatgc
      961 cctccagctc ggtcacgagg tcgggcgcgt aggcgcggtg cgcgtcgagg caggcatccg
     1021 ccagcgcgag gacgtcgcgc cggctcagct cgcggcccgt ccaggccgcg ccgccggcca
     1081 gggcgacgcg ctcggccgcg taagcgcgga tggcggagcg gaacgcctcg ccgtgaatcc
     1141 gcccgaagtc aaatggcgac cccttcaggt ctagggtagg taccgacatg gtgccaacat
     1201 cataggcccg ctcgggcgag ctccgagccg tggacagtca gtcgtcccgc tcaggcgagc
     1261 accgagccat gctcggtcgc ccagctgagg cggacgggcg tgccctccgc gagcatgccg
     1321 tcgaagccgt gcagttggtt ctgcacgcgc gccacgaccg tcacaccgcc cgccacctcg
     1381 acctggtagc gggcgtcggt gccgaggtag gtggcgcctg agaccctgcc cgggagcgcg
     1441 gattggcccg gccgcaggtc gggcggggcg tcggcctcga cgttctcgac catgagcttc
     1501 tcgggccgta gcgccagcgc cacgccgtcc ccgacgacga tctcctggtc gcgcaccacg
     1561 ccacgcagca cggtgccgtc tttcaacttc acgcttccct ccgagcccga cttcgcctcc
     1621 agcacgccgt ggaggaagtt cgtctcgccg atgaagtcgg ccacgaagcg cccggaaggc
     1681 cgctcgtaga tgtcctccgg tcggccgacc tgcaggacct cgcccatgtt catgacggcg
     1741 atgcggtccg ccatggtgag cgcctcctcc tggtcgtggg tgacgaacac gaacgtgatg
     1801 ccgacctgct gctgcaggcg cttcagctcg aactgcatgg cacgccggag cttgaggtcg
     1861 agggccccga gcggctcgtc gagcagcagc acgctcgggc ggttgacgag cgccctggcc
     1921 aacgccaccc gctgacgctg gccgccggag agctggaggg gcttgcgccg ctcgacgttg
     1981 gggaggcgca ccatctccag cgcctcgccc gcccgcgcgc gcgcctccgc ggcgggcacg
     2041 cgcgccatgc gcatgccgaa catgacgttc tggaggacgg tcatgtgcgg gaagagggcg
     2101 tagtcctgga agaccgtgtt gacgggccgg tggaacgacg ggatgccccg catggagcgc
     2161 ccgctgatca gcaactcgcc tgagtcgggc tcctcgaagc cggcgatgac ccggagcgtc
     2221 gtcgtcttgc cgcagcccga cgggccgagc agcgcgaaga actcg
//
LOCUS       DAOQYE010000296         2263 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_54148_length_2263_cov_2.415308, whole genome shotgun sequence.
ACCESSION   DAOQYE010000296 DAOQYE010000000
VERSION     DAOQYE010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54148_length_2263_cov_2.415308"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="PKN52_05690"
     CDS             complement(<1..358)
                     /locus_tag="PKN52_05690"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HNQ99471.1"
                     /translation="MTGTLGLFSLVDLFQLLAAAKRTGRLTVDHPSGLARVYFDRGRV
                     VHAEFCELTGERAVYALFADERGTFEFRIGMTAPSDSISVGTENLILEAVRLLDEERR
                     STGREEVPTLSRDAVPA"
     gene            483..1544
                     /locus_tag="PKN52_05695"
     CDS             483..1544
                     /locus_tag="PKN52_05695"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HNQ99472.1"
                     /translation="MPRRLQRALLRETAGLYPLGVAAICLLLCIDFLSVLARFLVEQD
                     ASVADIGALLLHRTPWFLHLALPVAVVFAVLITGGRLAKDSELKAAYAMGVPPMRLFW
                     PLVLFGLVVGALSLVNNGFLEPRGEAAYQRRIDAFVYARPPAEVQVDSAYRIEDAVYY
                     AARIRSLPGDHTLANLEGVVVQLADGTLYSARTGQWDSVAKTWRLTDAQVTRPGGSPT
                     RVGDQSLPFSLASSPNETLVRSASLSLDQVWSTLRSVERAGGDTRDLAYELHRRLADA
                     LSSPIFAAFAAALALRVRGRAAGFAWTIVLMVGFWATWLLSGNLFQAGAVGPLAAAWL
                     TPTLAAVGAGVLATRTLRS"
     gene            1541..>2263
                     /locus_tag="PKN52_05700"
     CDS             1541..>2263
                     /locus_tag="PKN52_05700"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HNQ99473.1"
                     /translation="MRRWGRYLVGETLPLFAVGVVALVLLLLLYFFLDVLADVLARGV
                     SVSLLARYLLYKLPAAAAPGLPLALLFASLLALTRLGEDGEVKAALLLGLPPRAFVLP
                     LVGLGLAVSVVALVNSELIVPWSERRAAEVEKDIMLLSPETLVTAGTFFTDSLGRSIF
                     IGAVGQGGRFEDVTVITPGGSSGPREVITAASGVPDPAAGVWRLEGIRMRVLRDARLT
                     VDTTAATAVLPVRGLAAGSTSNP"
BASE COUNT          289 a          812 c          789 g          373 t
ORIGIN      
        1 gggcgggcac ggcgtcgcgg gagagggtgg gcacctcctc gcggccggtg gagcgccgct
       61 cctcgtcgag cagacgcacc gcctccagga tcaggttctc cgtgccgacg ctgatggagt
      121 cgctcggagc cgtcatccct atgcggaact cgaaggtgcc gcgctcgtcg gcgaagaggg
      181 cgtagaccgc ccgctcgccg gtcagctcgc agaactcggc atggacgacc ctgccgcggt
      241 cgaagtagac gcgcgcgagc cccgatggat ggtccaccgt cagccgaccc gtgcgcttcg
      301 cggccgcgag gagctggaac aggtccacga gcgagaagag gccgagcgtg ccagtcacgg
      361 cggccatcct atcgcgggtg cgtcacgcct tcagtaaagc ccctctcatg cgccgcaggg
      421 cttgaatcgc ccgctcagca cgcgcatctc ggtatcacat gccacctcgg tatactcggc
      481 gggtgccgag acgcctgcag agagcgctcc tgcgtgagac ggccggtctc tacccgctcg
      541 gcgtggcggc catctgcctg ctcctatgca tcgacttcct gagtgtcctc gcgcgcttcc
      601 tcgtcgagca ggacgcctcc gtcgccgaca tcggcgcgct gctcctacac cgcacgccgt
      661 ggttcttgca cctcgcgctg cccgtcgccg tcgtgttcgc ggtgctcatc acgggcggca
      721 ggctagccaa ggacagcgag ctcaaggccg cgtacgcgat gggcgtgcca cccatgcgcc
      781 ttttctggcc cctggtgctc ttcgggctcg tcgtcggagc cctgtcgctc gtgaacaacg
      841 gcttcctcga accgcgcggt gaggccgcct accaacgccg gatcgacgcg ttcgtctacg
      901 cccgtccacc ggcggaggta caggtcgact cggcctaccg gatcgaggac gccgtctact
      961 acgcggcgcg catccgctcc ctccccggcg atcacacgct cgcgaacctc gagggcgtgg
     1021 tggtgcagtt ggccgacggt acgctctaca gcgccaggac cggccagtgg gactcggtcg
     1081 ccaagacgtg gcgcctgaca gacgcccagg tgacccgccc cggtgggtcg ccgacgcgcg
     1141 tcggcgacca aagcctgccg ttctcgctgg cgtcgagccc gaacgagacc ttggtgcgct
     1201 ctgcgagcct gagcctcgac caggtctgga gcaccctgcg ttccgtcgag cgggccggcg
     1261 gcgacacgcg cgacctcgcc tacgagctgc atcggcgcct ggccgatgcg ctcagttcgc
     1321 ctatcttcgc cgccttcgcg gccgcgctcg ccctgcgggt caggggtcgg gcggccgggt
     1381 tcgcatggac gatcgtcctg atggtcggct tctgggccac ctggctcctc tccggcaacc
     1441 tgttccaggc aggcgccgtc ggccccttgg cggcggcgtg gctgacaccg accctcgcgg
     1501 cggtcggagc aggagtgctg gcaacgcgga cgttgcgctc atgaggcgtt gggggcgcta
     1561 cctggtgggc gagacgctgc cgctcttcgc cgtcggtgtc gtggcgctcg tgctcctgct
     1621 actgctctac ttcttcctcg acgtgctggc ggacgtcctg gcgcgcggcg tcagcgtctc
     1681 gctcctggcg cgctacctcc tgtacaagct cccggcggcg gcggcgccgg gcctgccgtt
     1741 ggcgctcctg ttcgcctcgc tcctggcgct gaccaggctg ggcgaggacg gcgaggtcaa
     1801 ggccgccctg cttctcgggc tgccgccgcg agcgttcgtg ctgccgctcg ttggcctcgg
     1861 cctggcggtc agcgtcgtcg ccctggtgaa cagcgagctg atcgtgccat ggagcgagag
     1921 gcgcgccgcc gaggtggaga aggacatcat gctgctgagc ccggagacgc tcgtcacggc
     1981 cggcacgttc ttcactgact cgctggggcg gagcatcttc ataggcgccg tcggccaggg
     2041 cggccgtttc gaggacgtga ccgtcataac gccgggcggg agcagcggcc cgcgcgaggt
     2101 gataacggcc gccagcggcg tccccgaccc agcggcgggg gtatggcggc tggagggtat
     2161 caggatgcgc gtcctgcgcg acgcgcgtct gacggtcgac acgaccgcgg ccaccgccgt
     2221 cctgcccgtg cggggcctgg cggcgggctc cacctccaac ccc
//
LOCUS       DAOQYE010000297         2259 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_54307_length_2259_cov_2.438294, whole genome shotgun sequence.
ACCESSION   DAOQYE010000297 DAOQYE010000000
VERSION     DAOQYE010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54307_length_2259_cov_2.438294"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="PKN52_05705"
     CDS             complement(<1..394)
                     /locus_tag="PKN52_05705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99474.1"
                     /translation="MAGNAVGVRELRNHGGSVLERVQAGEEIVVNAPRTPIPDLDALP
                     FPAYEKLSGFPRGYNLPLFSYIRVPGATMITSRGCTFQCSYCDRSVFKRGYRWNSAAY
                     IYDHMRHLRMRFGVRHLNIYDDLFTANRP"
     gene            478..780
                     /locus_tag="PKN52_05710"
     CDS             478..780
                     /locus_tag="PKN52_05710"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNQ99475.1"
                     /translation="MYEDILPQDVAGWRERGAQVVDVREPWEFAAGHLPDSTNIPLGE
                     FVARLGELTAPLVLVCASGSRSASAAAYLDRRGFGEVANLDGGVHLWEDMGLELER"
     gene            complement(897..1802)
                     /locus_tag="PKN52_05715"
     CDS             complement(897..1802)
                     /locus_tag="PKN52_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNQ99476.1"
                     /translation="MGVEYVPYDPLRRVPTEHRDAEVLVAWLNPPSRLAEAATDLHEV
                     KLVQGLMAGANTLVAAGFAEDVPICNGRGLHDQPVAEHTVALLLAAARRLHLMRDAQH
                     ERRWSGELGGAQPDRNPAVFTTLDASNVTIWGFGSIAARLAPMLTALGARVRGVATAP
                     GTRNGYPVVGPDGLDGLLPDTDALVMILPASDATSGALDARRLQLLPRHAWVVNVGRG
                     NGIDEAALVRALEASELGGAALDVFSEEPLPAASPLWALPNVIISPHAAGGRPLGADA
                     FVRENVSRLLRGEPLLNVVDRAKGY"
     gene            complement(1874..2119)
                     /locus_tag="PKN52_05720"
     CDS             complement(1874..2119)
                     /locus_tag="PKN52_05720"
                     /inference="COORDINATES: protein motif:HMM:NF021758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rdx family protein"
                     /protein_id="HNQ99477.1"
                     /translation="MPKPTVQIQYCVPCGHLPRALDVQKSLLERFGQRIDGVMLKTGS
                     GGIFTIDVDGERIYTKPDEFDLDAIAQTIEARAAQAA"
BASE COUNT          371 a          733 c          791 g          364 t
ORIGIN      
        1 ggggacggtt ggcggtgaag aggtcgtcgt agatgttgag gtggcggacc ccgaagcgca
       61 tccggagatg gcgcatgtgg tcgtagatgt aggccgcaga attccatcga tacccccgtt
      121 tgaacacgga gcggtcacag taggagcact ggaaggtgca gccccggctg gtgatcatcg
      181 tggccccggg tacccggata tagctgaaaa gcggcaggtt gtagccccgg ggaaaaccgg
      241 agagcttttc ataggcggga aacggcaggg catcgagatc cgggatcggg gtccgggggg
      301 cgttgacgac gatctcctcc ccggcctgca cgcgctccag cacgctgccc ccgtgattgc
      361 gcagttctcg gacaccaacg gcgttacctg ccacatcgca atgtatcaca agtgagacga
      421 tgatccggtc gttcgtatgt aagcctctcg gacggccagc gcgactagaa tcgcgtggtg
      481 tacgaagaca tccttcccca agacgtagcc ggctggcgcg agcgcggcgc ccaggtggtc
      541 gacgtgcgcg agccgtggga gttcgccgcc ggacacctgc ccgactccac gaacatcccc
      601 ctgggcgagt tcgtggcgcg cctgggcgag ctgaccgccc cgctggtgct cgtgtgcgcc
      661 agcggttcgc gctccgcctc tgccgcggcc tacctggacc gccgcggctt cggcgaggtc
      721 gccaacctgg atggcggcgt tcatctatgg gaggacatgg ggctcgagct ggagaggtga
      781 aggtcgcgcg ccagaccgca cttggtctcg aagagcccct ctcaccaagc gccggttcgt
      841 gagccgccac cgtgtggcgc gcgggccgtg cgatgggtcg ctccgtaccc tttcgctcag
      901 taccctttgg cgcggtccac gacgttgagg agcggctcgc ctcgcagcag ccggctcacg
      961 ttctcgcgca cgaaggcgtc ggcgccaagc ggccgtccgc cggcggcatg cggcgagatg
     1021 atcacgttcg gcaaggccca gagcggcgac gccgccggga gcggctcctc cgagaacacg
     1081 tcgagcgccg caccgcccag ctcgctggcc tcgagcgcac gcacgagagc ggcctcgtcg
     1141 atgccgttgc cgcgcccgac gttgacgacc caggcgtgac ggggcagcag ctgcagccgt
     1201 ctggcgtcca gcgcgccgga tgtcgcgtcc gaggcgggca ggatcatcac cagcgcgtcc
     1261 gtatcgggca atagcccgtc cagcccgtca gggccgacaa ctggataacc gttgcgggtc
     1321 cccggagccg tggctacacc tctcaccctg gctccgaggg cagtgagcat gggcgcgagc
     1381 ctggcggcga tgctcccgaa gccccatatc gtcacgttgg aagcgtccag ggtcgtgaag
     1441 acagcggggt tcctatcggg ctgggcgccg cccagctcgc cggaccaccg gcgctcgtgc
     1501 tgagcgtcac gcatgaggtg aagccgacgc gcggccgcca ggaggagcgc gaccgtgtgc
     1561 tcagccacgg gctggtcgtg cagcccgcgg ccgttgcaga tgggcacatc ctcggcgaag
     1621 ccagcggcca cgagcgtgtt ggcgccggcc atcaggcctt ggaccagctt gacctcgtgg
     1681 aggtcggtcg ccgcctccgc aagccgagac ggcgggttca gccaggcgac gaggacctcg
     1741 gcgtcgcgat gctcagtggg aacgcgccgc agcgggtcgt acggcacgta ctcgacgccc
     1801 atatcccgcc tcgagaaatc gaacgtggcg ttggtcggga acaggacctt cgtggcggct
     1861 acctctgccg gacttaggcg gcctgcgcgg cacgcgcttc gatggtctgc gcgatcgcgt
     1921 cgaggtcaaa ctcgtccggc ttcgtgtaga tgcgctcgcc gtcgacgtcg atggtgaaga
     1981 tgccgccgga gcccgtcttg agcatgacgc catcgatgcg ctgaccgaag cgctcgagaa
     2041 gggacttctg gacgtcaagc gcgcgcggca gatgcccgca cgggacgcag tactggatct
     2101 ggacagtggg tttgggcatg gtgtttcttc ctctcgtcgt tggcgttgcc actacaggat
     2161 agttggccag agccgaacgt cgaggcagtc cggcgacagt aactactggc aacagtgcgc
     2221 cccgtacttc atggccctgg gtcacgcgtc gacaacagg
//
LOCUS       DAOQYE010000298         2257 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_54367_length_2257_cov_3.333333, whole genome shotgun sequence.
ACCESSION   DAOQYE010000298 DAOQYE010000000
VERSION     DAOQYE010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54367_length_2257_cov_3.333333"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1347
                     /gene="hutU"
                     /locus_tag="PKN52_05725"
     CDS             <1..1347
                     /gene="hutU"
                     /locus_tag="PKN52_05725"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HNQ99478.1"
                     /translation="NWEEFDRLDAAGLMMYGQMTAGSWIYIGTQGILQGTFETFAAAA
                     RRHFGGTLAGTLTVTAGLGGMGGAQPLAVTLNQGAVLVVEVDQHRIQRRLETGYLDQA
                     STDLDEALRSLEAAKSERRGLSVGLLGNAAEVLPELVRRGVAVDLVTDQTSAHDPLYG
                     YAPPALPDEDVATSRSTDPKAYRERVLQAMAKHCGAIVDLQRAGAIAFDYGNNLRAFA
                     REGGFDGAFAYPGFVPAFIRDQFCEGRGPFRWVALSGDPEDIRRTDRAILELFPNDIG
                     LRRWLTEGVSKFAFQGLPARICWLGYGERDKAGLRFNEMVASGELKAPIVIGRDHLDS
                     GSVASPYRETEAMQDGSDAVADWALLNFALNAVSGAGWVSFHHGGGVGMGYSLHAGQV
                     CVADGSAAAAERLARVLLNDPGTGVMRHADAGYDIAVGTARARGMDLPGVTEERPT"
     gene            1344..>2257
                     /locus_tag="PKN52_05730"
     CDS             1344..>2257
                     /locus_tag="PKN52_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HNQ99479.1"
                     /translation="MRRPAGPDADGVGRALVKGISELLTPHTAIIDAAMATEGERVAW
                     VGPESEVPAEYSRWPTLDLGGRAVIPGLVDSHTHLVWAGDRLDEYVLRSEGVSYAELL
                     AAGGGIQKSVASTRAATENGLLMLARRRAGSFLRGGVTALEIKSGYGLDTESELRMLR
                     VARQLGEDGPQRVTTTLLAHVPDPDVDRHVAVDRFVAETIPEAARTGLADAVDVFCDE
                     GAFTLAEAERILEAGLRYGLKVKAHAEQLTHTGAARLVAALGGVSADHLECATSDDLT
                     AMAEAGTVATILPVAAMLLRKPVPSASIL"
BASE COUNT          372 a          738 c          796 g          351 t
ORIGIN      
        1 aattgggagg agttcgaccg cctcgacgcc gccggcctga tgatgtacgg ccagatgacg
       61 gcgggctcgt ggatctacat cggcacacaa ggcatcctgc aaggcacgtt cgagaccttc
      121 gccgcggcag ccaggcgcca cttcggtggc acgctcgccg gaaccttgac ggttacggcc
      181 gggctgggcg ggatgggcgg agcgcagccg ctcgccgtca ccctcaatca gggtgccgtg
      241 ctggtggtcg aggtcgacca gcaccgcatc cagcggcgcc tggagaccgg ctacctcgac
      301 caggcgagca ccgacctcga cgaggctctg cggtccctcg aagccgccaa gagcgagcgg
      361 cgcggactgt cggtcggtct cctaggtaac gccgcggagg tcctgcccga gttggtgcgc
      421 cgtggtgtcg cggtagacct ggtcaccgac caaacgagcg cccacgaccc tctatacggc
      481 tacgccccac ctgccctgcc ggacgaggac gtggcgacaa gtcgctcaac cgacccgaag
      541 gcctaccgcg agcgtgtgct gcaggccatg gcgaagcact gcggcgccat cgtcgacctg
      601 cagagggccg gcgccatcgc gttcgactac ggcaacaact tacgggcgtt cgcacgcgag
      661 ggtggtttcg acggcgcctt cgcctacccg ggcttcgtac cggccttcat acgcgaccag
      721 ttctgtgagg gccgaggtcc gttcaggtgg gtcgcgctct cgggcgatcc tgaggacatc
      781 cgtcgcaccg accgagccat cctcgaacta ttccccaacg acatcggcct gcggcgctgg
      841 ctgacggaag gcgtgagcaa gttcgcgttc caaggcctgc cagcgcgcat ctgctggctg
      901 ggctacggtg agcgcgacaa ggcaggcctg cgcttcaacg agatggtcgc gagcggcgag
      961 ctcaaggcgc ccatagtgat cggccgcgac cacctcgact ccggctcggt ggccagcccg
     1021 taccgcgaga ccgaggcgat gcaagacggc tcggacgccg tggcggactg ggcgctcctg
     1081 aacttcgctt tgaacgccgt gagtggcgcc ggctgggtga gtttccacca tggagggggt
     1141 gtcgggatgg gctacagcct gcacgccggc caggtgtgcg tcgccgacgg ctccgcggcc
     1201 gcagccgagc gactggcgcg ggtgctgctc aacgaccccg gcacgggcgt catgcggcat
     1261 gcggacgccg gttacgacat cgccgtcggc acggcacggg ctcgggggat ggaccttcca
     1321 ggtgttacgg aagagcgtcc cacatgagac gcccggccgg ccccgacgct gacggcgtcg
     1381 ggcgcgccct cgtcaagggt atcagcgagt tgttgacgcc ccacacggcc atcatcgacg
     1441 cagcgatggc gaccgagggc gagcgcgtgg cctgggtcgg cccggagagc gaggtgcctg
     1501 cggagtactc tcgttggccg acgctcgacc tgggcgggcg tgccgtgatc ccagggctag
     1561 tagatagcca cacgcatctg gtgtgggctg gcgaccgtct ggacgagtac gtcttacggt
     1621 ctgaaggcgt gtcgtatgcc gagttgctcg cagcgggagg cggcatccag aagtcagtcg
     1681 cgtcgacccg cgccgctacg gagaacgggc tgctgatgct ggctaggcgc cgcgccggct
     1741 ccttccttag gggtggtgtg acggccctcg agatcaagtc aggctacggc ctggacaccg
     1801 agtccgagct aaggatgctg cgcgtggcga ggcagttagg cgaggacggc ccgcaaaggg
     1861 tgaccacgac gctgctcgcg catgtacctg accccgatgt cgaccgccac gtcgcggtag
     1921 atcgtttcgt cgccgagacc atccccgagg cggcgcgcac cggcttggcc gatgcggtag
     1981 acgtcttctg cgacgagggc gccttcacgt tggccgaggc agagcgcatc ctggaagcgg
     2041 gtctcaggta tggcctcaag gtgaaggcgc acgccgagca gttgacgcat acgggcgcgg
     2101 ccagactcgt ggccgcgctg ggaggagtct ccgccgatca cttggagtgc gcaacatccg
     2161 acgatctgac agccatggcg gaagcgggta ccgtggctac gattctgccg gtcgccgcca
     2221 tgctcctaag aaagcccgtg ccaagcgcaa gcatcct
//
LOCUS       DAOQYE010000299         2250 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_54642_length_2250_cov_2.866515, whole genome shotgun sequence.
ACCESSION   DAOQYE010000299 DAOQYE010000000
VERSION     DAOQYE010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54642_length_2250_cov_2.866515"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..646)
                     /locus_tag="PKN52_05735"
     CDS             complement(<1..646)
                     /locus_tag="PKN52_05735"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNQ99480.1"
                     /translation="MYTRNLRAPDKDFFLLGPRGTGKSTWLRANFPGALVVDLLAQEN
                     VVRYEREPGLLRSQVLARPTTDWIVIDEVQRVPKLLDEVQYLMEEEGYERFALVGSSA
                     RKLRRGGTDRLAGHPGLRHLYPLTARETGYAVPIEQLMRYGSLPLSVAAEDDDAREGF
                     LHSYATTYLVEEVRAEGLVRDLGSYSRFLEVAATSAGQRTNISALARDAGVARDT"
     gene            complement(844..1035)
                     /locus_tag="PKN52_05740"
     CDS             complement(844..1035)
                     /locus_tag="PKN52_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99481.1"
                     /translation="MKPFFVLLGVLLFLYVAACVLRGSVVVSWGPGARTFWRAEHPRW
                     FWTGVGIYALLALALVLVF"
     gene            1239..1691
                     /locus_tag="PKN52_05745"
     CDS             1239..1691
                     /locus_tag="PKN52_05745"
                     /inference="COORDINATES: protein motif:HMM:NF014082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNQ99482.1"
                     /translation="MIEAVGHVRGGRSDPTDDEWGRSRCAIELDRDRFGPEALTGLDA
                     FSHAEVVFLFDRVSPEKIELGARHPRGREDWPLIGIFAQRGKNRPNRLGVTVCKVLAV
                     EGTRLHVEGLDAIDGTPVIDIKPVMREFLPRGEVRQPDWVAELMTAYW"
     gene            1739..2149
                     /locus_tag="PKN52_05750"
     CDS             1739..2149
                     /locus_tag="PKN52_05750"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNQ99483.1"
                     /translation="MLPAHDAEALDRVRQLLEAQPDIRVAFAFGSVPAGRAGFESDID
                     IAVLAEEPLDVERTMELIRSIALATGRPVDLIDLRTAGPLISQEVLKHGTRLVCRDTV
                     ELADFIYRTLVDAADFLPLRARLQQARIDAWLRQ"
BASE COUNT          370 a          738 c          770 g          372 t
ORIGIN      
        1 ccgtgtcgcg cgccacgccg gcgtcgcgtg ccagggcgga gatgttcgtt ctctgcccgg
       61 ccgaggtcgc ggcgacctcc aggaagcggc tgtacgagcc gaggtcgcgc accaggccct
      121 cggccctgac ctcctcgacc aggtaggtcg tggcgtagga gtggaggaag ccctcccgag
      181 cgtcgtcatc ctcggcggcg acgctcagtg gcagggagcc gtagcgcatg agctgttcta
      241 tcgggaccgc gtacccggtc tcgcgcgccg tgaggggata caggtgccgc agccctgggt
      301 ggccagccag gcgatcggtc ccgccgcggc gtagcttacg ggcggacgag cccaccaagg
      361 cgaagcgttc gtagccttcc tcttccatca ggtactggac ttcgtcgagc aacttgggta
      421 cccgctgcac ctcgtcgatg acgatccagt cggtggttgg gcgcgcgagg acctgggacc
      481 gcagcaggcc cggctcgcgc tcgtaccgca cgacgttctc ttgcgcgagc aggtctacga
      541 ccagcgcgcc ggggaagttc gcgcgtagcc aggtgctctt cccggtgccg cggggcccga
      601 gcaggaagaa gtccttgtct ggtgccctca gatttcgcgt atacatcgac accaaattta
      661 ccgcaaaaat ggtggcgagg tagtcagcgt ttgtgagggt gttttccgtg ccttcaggac
      721 gaccgcatgg tccctccctt cacgacgacc gcacggtccc gcccttaaca atgaccgcac
      781 ggtcccgcct ggacgcgcga tgcttcgtcg gtcggtggct gaagctcagc ggaccatagg
      841 ccctcagaac acgagaacta gcgccagcgc caacagcgcg tagatgccca cgcccgtcca
      901 gaaccaccga gggtgctccg cccgccagaa ggtgcgcgcg ccgggccccc acgacacgac
      961 gacgctgccc cgcagcacgc acgctgccac gtacaggaag aggagtacgc ccagcagcac
     1021 gaagaacggc ttcatgggtg gatggtagag cccgggcagc ttgctgatgg tggcggatgc
     1081 cgcactgggg tcggccgcgc ttgggtcggc tgcacatcac ttcggccgcg catcacttcg
     1141 accgggtcgg ccacacgagg ccgtacgctg cgcgtggagc tcgccgcatc tcccacgaaa
     1201 cggtgagtgc ctccgggagg ctcaacatgg tggagtacgt gatcgaagcc gtcggacacg
     1261 tgcgcggcgg caggagcgac ccgaccgacg acgagtgggg gaggagccgt tgcgccatcg
     1321 agctggaccg tgaccgcttc ggcccggagg cgctaaccgg attggacgcc ttcagccacg
     1381 cggaggtcgt cttcctcttc gacagggtgt cgcctgagaa gatcgagctg ggcgcccggc
     1441 acccacgcgg ccgggaggat tggcccctca tcggcatctt cgcgcagcgc ggcaagaacc
     1501 ggccgaaccg gctcggggtg accgtctgca aggtcctggc ggtcgagggt acccggttgc
     1561 acgtcgaggg cctggacgcg atagacggca ctcccgtgat cgacatcaag cccgtgatgc
     1621 gcgagttctt gccccgcggg gaggtcaggc agcccgactg ggtggcggag ctgatgacgg
     1681 cttattggtg atgtggggcg cggcggcgct cggtacttgc cggtgctatc cttccgccat
     1741 gcttcccgcg cacgacgccg aggcgctcga ccgtgtgcgc cagctgttgg aggcgcaacc
     1801 cgacatccgg gtcgcgttcg ccttcggctc ggttccggcg ggtagggcag gtttcgagag
     1861 cgacatagac atcgccgtgc tggctgagga gccgctcgac gtcgagcgca ccatggagct
     1921 catccgttcc atcgcgcttg caacagggag gccggtcgac ctgatcgacc tgcggactgc
     1981 cggaccgcta atctctcaag aggtgctgaa gcacggcacc cggcttgtgt gccgcgacac
     2041 cgtggaactg gccgacttca tctaccgcac gctcgtcgat gcggcagact tcttgccgct
     2101 tcgcgcgcgg cttcaacaag cgcgcataga cgcgtggctt cggcagtagt tcacgagaag
     2161 gtcgcttccc tcatgcgttg cgtagcgcgc atcgaggggg cgcgagctgc cacgctcgac
     2221 gtattggctc acgacataga ccgtcaggac
//
LOCUS       DAOQYE010000300         2250 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_54658_length_2250_cov_2.272893, whole genome shotgun sequence.
ACCESSION   DAOQYE010000300 DAOQYE010000000
VERSION     DAOQYE010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54658_length_2250_cov_2.272893"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="PKN52_05755"
     CDS             <1..440
                     /locus_tag="PKN52_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HNQ99484.1"
                     /translation="LGAGRRVRVASYEAEGPVLERRGDQVVVQLGLIKVTVPVTDVKP
                     AKPASGSAARRTPVAIGQASAPRELNLRGERVEAGLELLRDFILEAHALKLESVRVLH
                     GKGTGALRDAVRSYLKGEKLVERFEDAVPYEGGHGVTVAYLRR"
     gene            572..1201
                     /locus_tag="PKN52_05760"
     CDS             572..1201
                     /locus_tag="PKN52_05760"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HNQ99485.1"
                     /translation="MPGKLAIVFDLDGTLVDSLDDILDTFLATFEELGGEPPERGAVR
                     ALVGRPLDEMYAGFVPAQRLGTYVAAYRERYRRHLANKTRPYPGVVDLLSELGERGYA
                     RLVATTKRSATANELVEAVGLADYVDHVQGTDGFPHKPAPDVILRALATADAHGTWMV
                     GDTTSDIVAGRAAGLKTFGVTWGTHDAATLAAARPDAVEPTIGPLLLLL"
     gene            1293..1979
                     /locus_tag="PKN52_05765"
     CDS             1293..1979
                     /locus_tag="PKN52_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HNQ99486.1"
                     /translation="MNGIEKQYVIDALAEDSWRMFRIIGEFVQGFEEMADVGKAVTVF
                     GSARLKEGTEYYAKAQQLGGALAREGYTVLTGGGPGLMEAGNRGAKEAGGRSMGLNID
                     LPHEQSPNPYQTDTLQFKYFFVRKVMLVKYSTAFVVFPGGFGTIDELFEALTLIQTRK
                     IKPFPVYLVGVEFWRGLLQWLQGTLLRAGTISDGDQHLFKVVDDVSTIPAEIEAYYRN
                     STQAGFNLPW"
     gene            complement(2046..>2250)
                     /locus_tag="PKN52_05770"
     CDS             complement(2046..>2250)
                     /locus_tag="PKN52_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99487.1"
                     /translation="VRQGDVIATTGNSGTAEAAEGTQDDPHPHVEVWRGDTTFLGAAM
                     EPEQIWELAAQVFGVAALPPFHD"
BASE COUNT          350 a          788 c          767 g          345 t
ORIGIN      
        1 ccttgggcgc cggacgacgt gtgcgcgtcg cgagctacga ggccgagggg cccgtgctgg
       61 agcgcagggg agaccaggtc gtcgtgcaac tcggcctcat caaagtcacg gtgcccgtga
      121 cggacgtcaa gcccgccaag ccggcgagcg gcagcgcggc gcggcgcacg cccgtcgcca
      181 tcggccaggc gagcgcgccc cgcgagctca acctgcgggg cgaacgggtg gaggccggcc
      241 tcgagctcct gcgcgacttc atcctcgagg cgcacgccct gaagctcgag tcggtgcgcg
      301 tcctgcacgg taagggcacc ggagcgttgc gagacgccgt ccgcagctac ctgaagggcg
      361 agaagctcgt agagcgcttc gaggacgccg tgccttacga ggggggccac ggcgtcaccg
      421 tcgcttacct gcgccgttga gcgcccggcg tgccgcgacc cggttcccga cagacggacg
      481 gccgatccgt cgctaccctc tcgcctgacg gccgcggtct gccgcggtcc ctctgcccga
      541 cccgcgacca cgcatctgcg atactcgccc catgcccggt aaactcgcca tcgtcttcga
      601 tctcgacggc accctggtcg actccctcga cgacatcctc gacacgttcc ttgccacctt
      661 cgaggagctg ggcggtgagc cacccgagcg cggcgccgtg cgagcgctcg taggcaggcc
      721 gttggacgag atgtacgcgg ggttcgtccc ggcgcagagg ctcgggacct acgtcgccgc
      781 ctaccgcgag cgctaccggc gccacctcgc caacaagacc cggccctacc cgggtgtcgt
      841 ggacttgttg agtgagctgg gcgagcgcgg ctacgcgcgg ttggtggcga ccaccaagcg
      901 ctccgccacc gccaacgagc tggtggaggc cgtcggcctc gcggactacg tcgatcacgt
      961 gcagggcact gacggcttcc cgcacaagcc ggcgccagac gtgatcctgc gcgccctggc
     1021 cacggccgac gcgcacggca cgtggatggt gggcgacacg accagcgaca tcgtcgcggg
     1081 cagggctgcc ggtctcaaga cgttcggcgt cacatgggga acgcacgacg cggccacgct
     1141 cgccgccgcc cgtcctgacg ccgtcgaacc gaccatcggt ccgctcctgc tcctcctgtg
     1201 aggcaaagcg cggctgcttc cgccggcgag ccgagcccac gtgtgaaacg acagtgatcc
     1261 gtcggcgagc ccttgtcgat taacctagcg agatgaacgg catcgagaag cagtacgtga
     1321 tcgacgccct cgccgaggac tcatggcgca tgttcaggat catcggtgag ttcgtgcagg
     1381 ggttcgagga gatggcggac gtggggaagg ccgtcacggt cttcggctcg gcccgtctca
     1441 aggagggcac ggagtactac gccaaggcgc agcagctggg cggggcgctg gcacgcgagg
     1501 gctacacggt ccttaccggc ggcggccctg gcttgatgga agcgggcaac cgcggggcca
     1561 aggaggccgg cggccgcagc atgggcctca acatcgacct gccgcacgag cagtcgccga
     1621 acccgtacca gacggacacg ctccagttca agtacttctt cgtgcgcaag gtcatgctcg
     1681 tcaagtactc gacggctttc gtcgtgttcc cgggcggctt cggcaccatc gacgaactct
     1741 tcgaggcgct gacgctcatc cagacccgca agatcaagcc gttccccgtc tatctggtcg
     1801 gcgtggagtt ctggcgcggc ctcctgcagt ggcttcaggg gacgttgctg cgcgctggca
     1861 cgatctccga cggtgaccag cacctcttca aggtcgttga cgacgtgagc acgatcccgg
     1921 ccgagatcga ggcgtactac cgcaactcta cgcaggcggg cttcaacctg ccctggtagg
     1981 cgggccgttc ttccgtcgcg gcgtgggcca agggctcgct cgacgtcgag cgagcccttg
     2041 gctcgtcagt cgtggaaggg cggtaacgcc gcgacgccga acacctgcgc cgccagctcc
     2101 cagatctgct ccggctccat ggccgcgccg aggaaggtcg tgtcgccgcg ccacacctcg
     2161 acgtgcgggt gcgggtcgtc ctgcgtgcct tccgcagcct cggcggtgcc agagttgccc
     2221 gtcgtggcga tgacgtcacc ctgcctgacc
//
LOCUS       DAOQYE010000301         2244 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_54868_length_2244_cov_3.391046, whole genome shotgun sequence.
ACCESSION   DAOQYE010000301 DAOQYE010000000
VERSION     DAOQYE010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54868_length_2244_cov_3.391046"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="PKN52_05775"
     CDS             complement(<1..267)
                     /locus_tag="PKN52_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HNQ99488.1"
                     /translation="MEATESSAPAATAERSVAPNFVTDIIDRDIASGAVRQVVTRFPP
                     EPNGYLHIGHAKAICLDFGVAKDYGGVTYLRFDDTNPATEDPEYV"
     gene            complement(402..1292)
                     /locus_tag="PKN52_05780"
     CDS             complement(402..1292)
                     /locus_tag="PKN52_05780"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HNQ99489.1"
                     /translation="MNSSASDTTGRSRGRGHLPRILSALLAFGATVVVLWVGLPLIAP
                     AYLFLCFVAIQEYTALVGLRGIPIRKRSLWVATVLTLPASLPVTYPGMQPLMAGVSWR
                     EALIGLVALYLVALELANPNENSLQAVVFSLFGYLYIPWFFGFLITLRYTPDPVLGLW
                     YLMLPALAVVATDVGAFTFGSLFGKRKLAPSVSPNKTVEGSIGGLVLAAVVVGGVLFL
                     LEDHTGLHVNVYDGLLFSILVSSSAQLGDLFESLLKRWAGVKDAGVFLPGHGGVLDRI
                     DSMIVALPMTYFFVSLVILR"
     gene            complement(1289..1843)
                     /gene="frr"
                     /locus_tag="PKN52_05785"
     CDS             complement(1289..1843)
                     /gene="frr"
                     /locus_tag="PKN52_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HNQ99490.1"
                     /translation="MTDDLNDARERMQKSLDAFETNLATVRTGRANPAVLGRVMVEYY
                     GATVPLNQVANVSTPDARTLLITPFDKSAAKGIEKAILEADLGFNPSNQGDSIFITVP
                     PLNDERRRDLVKTVHQLSEEAKVGVRNIRRDCLSDLRAMQKEGDLGEDDLRREEGEVQ
                     KLTDEFIAKIDARVKSKETDILAV"
     gene            complement(1914..>2244)
                     /locus_tag="PKN52_05790"
     CDS             complement(1914..>2244)
                     /locus_tag="PKN52_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99491.1"
                     /translation="TDTTAALRALEIDADMVLMAKNKVDGVYDADPNKDPNARRYRSL
                     SYMDVLNGGLAVMDATAVSLCMQRDMPVTVFDIFSAGNLGRLLAGERVGTLIAPDTVK
                     EYAPGAQ"
BASE COUNT          354 a          700 c          817 g          373 t
ORIGIN      
        1 gacgtactcg gggtcctcgg tggcggggtt ggtgtcgtcg aagcgcaggt acgtgacgcc
       61 gccatagtcc ttggcgacgc cgaagtcgag gcagatggcc ttggcgtgcc cgatatgaag
      121 gtagccgttc ggttcggggg ggaagcgggt cacgacctgc cgcacggctc cgctggcgat
      181 gtcgcggtcg atgatgtccg tgacgaagtt cggggctacg gaccgctccg ccgtcgcggc
      241 gggcgcgctt gactcggttg cttccacgtc tactcgggct cctcgttcgg gcggtactgc
      301 gcccctgagc gtccacccgt cgcgcctggc ggccgggagg gggcgccagg cgcgaggcag
      361 gggcctcgcg cgcagcgcag gtcgcctact ctaccgcgcg ctcagcgcag tatgacgaga
      421 ctgacgaaga agtaggtcat gggcagcgcg acgatcatgc tgtcgatccg gtcgagcacg
      481 ccgccgtgcc cggggaggaa gacgccggcg tccttgacgc ccgcccaccg tttgaggagc
      541 gattcgaaga ggtcgcccag ctgcgccgag ctgctcacga ggatgctgaa gagcaggccg
      601 tcgtagacgt tgacgtgcag gcccgtgtgg tcctcgagga ggaagagcac cccgccgacg
      661 acgaccgcgg cgaggacgag accgccgatc gagccctcga ccgtcttgtt gggtgagacg
      721 gagggcgcga gcttgcgctt gccgaagagg gagccgaagg tgaacgcgcc gacgtcggtg
      781 gcgaccacgg ccagggcggg cagcatcagg taccagaggc cgagcacggg gtcgggagtg
      841 tagcgcaggg ttatcaggaa gccgaagaac caggggatgt agaggtagcc gaacagcgag
      901 aagacgacgg cctgcaagga gttctcgttg gggttggcca gctcgagcgc aacgaggtaa
      961 agggccacga ggccgatgag ggcctcgcgc cagctcacgc ccgccatcag gggttgcatg
     1021 ccagggtacg tgacgggcaa cgaggcaggc aacgtgagca cggtggcgac ccacaggctg
     1081 cgcttcctta tgggtatgcc gcgcagcccg acgagcgccg tgtactcctg gatggccacg
     1141 aagcacaaga agaggtaggc cggcgctatg agcggcaagc ctacccacaa gaccaccacc
     1201 gtcgctccga acgccaagag ggcggagagg atgcgcggca gatggccccg ccctcgcgac
     1261 cgccctgtgg tgtccgaggc ggacgagttc acaccgctag gatgtccgtc tccttggact
     1321 tgacgcgcgc gtcgatcttg gcgatgaact cgtcggtgag cttctgcacc tcgccctctt
     1381 cgcggcgcag gtcgtcctcg ccgaggtcgc cttccttctg catggcgcgc aggtcagaca
     1441 ggcagtcgcg gcggatgttg cggaccccga ccttggcctc ttcgctcagc tggtggacgg
     1501 tcttcacgag gtcgcgccgc cgctcgtcgt tgagtggcgg gaccgtgatg aagatgctgt
     1561 caccctggtt gctcgggttg aagccgaggt cggcctcgag gatggccttc tcgatgccct
     1621 tggcggcgct cttgtcgaac ggcgtgatca gcagcgtcct ggcgtcgggc gtcgagacgt
     1681 tcgccacctg gttgagcggc acggtggcgc cgtagtactc caccatcacc cgcccgagga
     1741 cggcggggtt ggcacgcccc gtgcgcaccg tcgcgaggtt cgtctcgaac gcgtcgaggc
     1801 tcttctgcat gcgctcgcgc gcgtcgttga ggtcgtcagt cattacgcca cctccggggc
     1861 ctgcaggcgc cttggaagag tagtttaccc cgttcccgtc gggtccgact cggtcactgc
     1921 gcgcccgggg cgtactcctt gacggtgtcg ggtgcgatga gcgtgccgac gcgctcgccg
     1981 gcgagcaggc gacccaagtt gccggcgctg aagatgtcga agacggtgac gggcatgtcg
     2041 cgctgcatgc agagcgacac ggccgtggcg tccatgaccg ccagcccgcc gttcaggacg
     2101 tccatgtacg agagcgagcg gtagcgcctg gcgttcgggt ccttgttcgg atcggcgtcg
     2161 tacacgccgt cgaccttgtt cttggccatc agcaccatgt cggcgtctat ctcgagggcg
     2221 cgcagggcgg ccgtggtgtc ggtc
//
LOCUS       DAOQYE010000302         2243 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_54916_length_2243_cov_2.868830, whole genome shotgun sequence.
ACCESSION   DAOQYE010000302 DAOQYE010000000
VERSION     DAOQYE010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54916_length_2243_cov_2.868830"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(146..1417)
                     /locus_tag="PKN52_05795"
     CDS             complement(146..1417)
                     /locus_tag="PKN52_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HNQ99492.1"
                     /translation="MNDEPPAAESAAAEELADGSQPFDLTALADGQPVEVVERDGVRY
                     TLLGTAHVSKSSAAAVTRLVESGRFDAVAIELDAGRYRSLSDPDAYSKMDLFKVLREG
                     KAGMLAVNLALSAFQQRMAEQFGVEPGAEMKAAMHAAKAANLPLLLIDRDIGVTLRRV
                     YANVPWWQRLTLFGGLLASAFSNEKISEEEVEALKEGDLLESTFNEFASKSERIYVPL
                     IKERDEYMVARLVQQGRGHREVLAVMGAGHLKGVAAGLAAAAPDVGSQVSRLEQVPPP
                     GKVGKVLPWAIVALVLLGFVIGFLRSPDIGLKLLTDWTLIHAVLSAAGALAALAHPIT
                     VLVAAVASPFTALNPLIGVGFVAAGTELWLKRPSVGDFEALRHDVTTWRGWWRNRAAR
                     VLLVFILTTIGSASGTYIAGFRIFGRLFGGG"
     gene            1550..>2243
                     /locus_tag="PKN52_05800"
     CDS             1550..>2243
                     /locus_tag="PKN52_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99493.1"
                     /translation="MTTLLKRLFARAPGGTLSQQVRSARLWLPLVIVGVVVVYQVFIV
                     PMGGPAFEFWATILFYGILGPIVTYATLNWIAEEVKLHEQGQADLARVYQELSDSHEL
                     LGAIQDVTMRYAAAPDIEAIIATAARGVAKVTGARAVSLVVGPSELGASHGVGLTPEL
                     EADALRRDRQLRLAETGQEGPTQVSTVGGAANGPLVVLSRPLSWGDRSERSLHAYYAA
                     EPDARSREAFDIL"
BASE COUNT          334 a          806 c          774 g          329 t
ORIGIN      
        1 tgcgtgctcg cctccgacct cgccgactac cgcttcgtct tcgtcgacgg ttacgcccag
       61 tgggcgatgg acggtgcgcc ggcaactccc tgaagcccgg tcgggctccc tgccggcgcc
      121 ggcagggagc cgatccatga tggcctcagc cgcccccgaa caggcggccg aagatccgga
      181 agccggctat gtaggttccg ctggcggagc cgatggtcgt gaggatgaac acgagcagca
      241 ccctcgccgc ccggttccgc caccagccgc gccacgtcgt cacgtcgtgg cgcagggcct
      301 cgaagtcgcc gacgctcggc cgcttcagcc acaactccgt gcctgccgcc acgaacccga
      361 cgccgatgag agggttgagg gccgtgaatg gcgacgccac ggccgcgaca agcacggtga
      421 tcggatgcgc gagggcggct agcgcccccg cggcgctcag caccgcgtgg atcagcgtcc
      481 agtcggtcag gagcttgagc ccgatgtcgg ggctgcgcaa gaagccgatg acgaagccga
      541 ggaggactag ggcgacgatg gcccacggca ggaccttgcc gaccttgccg ggcggcggca
      601 cctgctccaa ccgactcact tgggagccga cgtccggagc cgccgccgcg aggccggcgg
      661 ccacgccctt gaggtggcct gcccccatca cggcgaggac ttcgcgatgg ccgcgcccct
      721 gctggacgag ccgcgccacc atgtactcgt cgcgctcctt gatgagcggg acgtagatgc
      781 gctcgctctt gctcgcgaac tcgttgaacg tcgactcgag caggtcgcct tccttgagcg
      841 cctcgacctc ctcctccgag atcttctcgt tgctgaacgc ggaggcgagc agcccgccga
      901 agagggtgag gcgctgccac caggggacgt tggcgtagac gcgccgcagc gtcacaccga
      961 tgtcgcggtc gatgagaagc agcggtaggt tggcggcctt ggcggcgtgc atggcggcct
     1021 tcatctccgc gcccggctcg acgccgaact gctccgccat gcgttgttgg aaggcagaaa
     1081 gcgcgaggtt gacggcgagc atgccggcct tgccctcgcg cagcaccttg aagaggtcca
     1141 tcttcgagta cgcgtccggg tcggagaggg agcggtagcg gccggcgtcg agctcgatcg
     1201 ccacggcgtc gaagcgaccg ctctcgacta gccgcgtcac ggcggcggcg ctcgacttcg
     1261 agacgtgggc ggtccccagg agcgtgtagc gcacaccgtc gcgctccacg acctcgaccg
     1321 gctggccgtc ggccagcgcc gtcaggtcga agggttgcga cccgtcagcc agttcctcag
     1381 cagcggcgct ctcggccgcg gggggctcat cgttcatgcc ggagtgtacc gtcgcgacca
     1441 cccgggggca gtccgggccg ccacccgact agaatcgacg catgcgtaga gacgaccctg
     1501 cacggccggc gcgatccgcc cggctccgtc actcgcgccg gtggcgcgag tgacgacgct
     1561 cctcaagcgc ctgttcgcgc gcgctcccgg cggcaccctc tcgcaacagg tgcgaagcgc
     1621 gcgcctctgg ttgcctctcg tcatcgtggg cgtcgtcgtg gtctaccagg tcttcatcgt
     1681 gccgatgggc ggccccgcct tcgagttctg ggcgaccatc ctcttctacg gcatcctcgg
     1741 tccgatcgtg acctacgcga ccctcaactg gatcgcggag gaggtcaagc tccacgagca
     1801 ggggcaggcc gacctggcgc gcgtctatca ggagctcagc gacagccacg agctcctcgg
     1861 cgccatccag gacgtgacca tgcgctacgc ggccgctccc gacatcgagg cgatcatcgc
     1921 cacggcagcc cgcggggtcg ccaaggtgac cggtgcgcgc gccgtcagcc tcgtcgtcgg
     1981 accgtccgag ctgggcgcct cgcacggcgt aggactcacg cccgaactcg aggctgacgc
     2041 cttgcggcgc gaccggcagt tgcgcctggc cgagaccggc caggagggcc cgacgcaggt
     2101 gagcacggtc gggggtgccg ccaacggccc gctggtcgtc ctcagccgcc cgctgtcctg
     2161 gggcgacagg tcggagcggt cgctgcacgc ctactacgcg gcggagccgg acgcccgcag
     2221 ccgcgaagcc ttcgacatcc tcg
//
LOCUS       DAOQYE010000303         2242 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_54976_length_2242_cov_2.336534, whole genome shotgun sequence.
ACCESSION   DAOQYE010000303 DAOQYE010000000
VERSION     DAOQYE010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54976_length_2242_cov_2.336534"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            233..394
                     /locus_tag="PKN52_05805"
     CDS             233..394
                     /locus_tag="PKN52_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99494.1"
                     /translation="MLESADADVLCELAERLGDDLECWEAAERTLLPGDPRLTVARAR
                     VRRLRLAYL"
     gene            complement(430..2142)
                     /locus_tag="PKN52_05810"
     CDS             complement(430..2142)
                     /locus_tag="PKN52_05810"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ99495.1"
                     /translation="MLRRFFTYYAPYRGLFALDFTSAVVAGVLELAFPLAVQLFIDRL
                     LPTGHWVSIAFAAGALALVYLLNTGLMATVTYWGHMLGINIETDMRRKAFDHLQKLSF
                     GYYDEQKTGHLVARLTKDLEEIGEVAHHGPEDLFIAIMTLIGAFVLMFTVSVPLALIT
                     LVVVPFTGWFTVRYGGRMTQNWRAIYDRVGAFNGRIEENVGGIRVVQAFANEDHEREL
                     FSHDNHAYRTTKLEAYRIMAATNAFSYLSMRFTQLIVMVAGSYFVVNGTLSNGGFVAF
                     ILLVGVFFRPLEKINAVIETYPKGIAGFKRYTELIDTRPDIVDRPGAADVASLRGDIR
                     YEGVSFSYAKGKTVLAEIDLSIAAGETVAFVGPSGAGKTTLCSLLPRFYEPTAGRITV
                     DGIDVRDMTLASLRRQIGIVQQDVFLFAGTIRENIAYGKLGASEAEIMAAARRARLDE
                     MIAELPNGLDTIIGERGVKLSGGQKQRLAIARIFLKNPPILILDEATSALDTQTERAI
                     QQSLSELAVGRTTLVIAHRLATVKDADRIVVVDETGVAEQGTHGELLAHGGLYSVLHR
                     AQSV"
BASE COUNT          355 a          719 c          801 g          367 t
ORIGIN      
        1 gagcggcgtc cgcgccatga tgacgaggct gaggaagctg ctcccggtga cggacgcccc
       61 ctaactgctc gccgagccgt tcacggcgga cacgctggcg cttcgtgacc atctcgccag
      121 gaacgaggtc cggcaggccg tcgccctcta ccggtcgggg ctactgccgc ggagttcggc
      181 gcctggagcg gtcgaggaac ggctcgtcct ggaggaggaa ctgaggcagg ccgtgctcga
      241 gtcggccgac gcggacgtgc tttgtgagtt ggcggagcgg ttgggcgacg acctcgagtg
      301 ttgggaggcg gccgagagga cgctgctgcc cggcgacccc cgtctgaccg tcgctcgcgc
      361 gcgcgtcagg cggttacggc tggcctacct gtgaacgaca cggccaccgt cggcacccgg
      421 cttctccgct cacacgctct gcgcccggtg cagcacgctg tacagcccgc cgtgtgcgag
      481 cagctctccg tgcgtgccct gctccgccac cccggtctcg tctaccacca cgatgcgatc
      541 ggcgtccttc acggtggcca ggcggtgggc gatgacgagc gtcgtgcgtc cgaccgcgag
      601 ttccgagagg gactgctgga tggcccgctc cgtctgcgtg tcgagcgccg acgtcgcctc
      661 gtcgaggatg aggatgggcg ggttcttgag gaagatgcgg gcgatggcca ggcgctgctt
      721 ctggccgcct gagagcttca ccccgcgctc gccgatgatc gtgtcgagcc cgttgggaag
      781 ctccgcgatc atctcgtcga gccgcgcgcg cctggccgcc gccatgatct cggcctccga
      841 ggcgccgagc ttgccgtagg cgatgttctc gcggatggtg ccggcgaaga ggaacacgtc
      901 ctgctgcacg atgccgatct ggcgcctgag gctggcgagc gtcatgtcgc gcacgtcgat
      961 gccgtccacg gtgatccgcc cggcggtcgg ctcgtagaag cgcggcagga gcgagcacag
     1021 cgtcgtcttg cccgcgcccg agggaccgac gaacgccacc gtctcgccgg cggcgatact
     1081 caggtcgatc tcggccagga ccgtcttgcc cttcgcgtaa ctgaacgaca cgccctcgta
     1141 gcggatgtcg ccgcggaggc tggcgacgtc ggcggcgccg gggcggtcga cgatgtcggg
     1201 gcgcgtgtcg atgagctccg tgtagcgctt gaagccggcg atgcccttcg ggtaggtctc
     1261 gatcacggcg ttgatcttct cgagcggcct gaagaacacg ccgacgagga ggatgaaggc
     1321 cacgaacccg ccgttggaga gcgtgccgtt cacgacgaag tagctcccgg ccaccatcac
     1381 gatcagctgc gtgaagcgca tgctcaggta cgagaacgcg ttggtggcgg ccatgatgcg
     1441 gtacgcctcg agcttcgtcg tgcggtaggc gtggttgtcg tgggagaaga gctcccgctc
     1501 gtggtcctcg ttggcgaacg cctgcacgac gcggatgccg ccgacgttct cctcgatgcg
     1561 tccgttgaac gcccctaccc ggtcgtagat ggcgcgccag ttctgcgtca tgcggccgcc
     1621 gtagcgcacg gtgaaccagc ccgtgaacgg cacgacgacc agcgtgatga gcgcgagcgg
     1681 cacgctcacg gtgaacatga gcacgaacgc cccgatgagc gtcatgatcg cgatgaagag
     1741 gtcctcgggg ccgtggtgcg cgacctcgcc gatctcctcg aggtccttcg tgagccgcgc
     1801 gacgaggtgc ccggtcttct gctcgtcgta gtagccgaac gagagcttct gcaggtggtc
     1861 gaacgccttg cgccgcatgt cggtctcgat gttgatgccg agcatgtgac cccagtacgt
     1921 gaccgtcgcc atgaggccgg tgttcagcag gtacacgaga gcgagggccc ccgccgcgaa
     1981 cgcgatggag acccaatggc cggtaggcag gagccggtcg atgaagagct gaacggccag
     2041 cgggaaggcg agttcgagca cgccggcgac gacagcgcta gtgaagtcga gcgcgaagag
     2101 ccccctatac ggggcgtagt aggtgaagaa acgccgcagc acccggagat gctacatgag
     2161 cgctggcctc ctcaacgttc gtagaagggt ggcggctcga cgccgagggc gcgcaggtag
     2221 acgtaacctt ggccgcggtg at
//
LOCUS       DAOQYE010000304         2241 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_54998_length_2241_cov_3.362306, whole genome shotgun sequence.
ACCESSION   DAOQYE010000304 DAOQYE010000000
VERSION     DAOQYE010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54998_length_2241_cov_3.362306"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="PKN52_05815"
     CDS             complement(<1..403)
                     /locus_tag="PKN52_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99496.1"
                     /translation="MATKRPARSLRLLSAMALGVMMTALASAQGTDQADLPVTRVVLF
                     TNGVGYFEHSGTVVGNQVMNLQVPREEMDDMLQSLVLQDFGGGAIKPVRYSARDPLER
                     VLDGYRVDVSQNLTLEGVLAQARGEVVELVGG"
     gene            complement(482..1792)
                     /locus_tag="PKN52_05820"
     CDS             complement(482..1792)
                     /locus_tag="PKN52_05820"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNQ99497.1"
                     /translation="MSPLARNGARPGHDHGADTRSGTDAGARPPRARERLLVRGGTLV
                     LPDRLAPDTVLLVADGVIAGIAPESELTGDQLDARTVDARGAYVLPGLVDIHTHGAGG
                     HLFNEADDAAWGTVLATQLGRGVTSVLPTTLTASIPELTAALAQGRKWLGAQAGGAAR
                     GGEAPAHAQVLGMHVEGPYFALAQSGAQDPAHIRNPDDGTVDELLAYADAIRLMSFAP
                     ELPGALELASRLRALGIVPAAGHSSARDEHLAAAMERGLTHVIHLFSAQSTTVREGPW
                     RKPGLLEASLAFDGLTVEVIADHRHLPTTLLRLAYKAIGPERLCIVSDATHGAGMPEG
                     TELKLGSLDIVVSGGVAMLKDLTAFAGSTTLLDRMLQVMHFEVDLPLVEVVRMASLNP
                     ARAVGAAEHKGSLEVGKDADFALFSPGLEPLAVYVGGERAAPKV"
     gene            complement(1789..>2241)
                     /locus_tag="PKN52_05825"
     CDS             complement(1789..>2241)
                     /locus_tag="PKN52_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99498.1"
                     /translation="TAVGADGRVHVVSLSEEPGDTALPLAELTRATLNVTATAMRSDP
                     EHSSYEFQGTFLPDLPGAAPLPIYGHVWTSRFHDYRLGPASIPPPRLTGSARVVPAGG
                     AEEDDALFTLSFDSLDRQATTYDGSLTDSRRGGDGEMSYMITLRAAAR"
BASE COUNT          318 a          768 c          808 g          347 t
ORIGIN      
        1 gaccgccgac gagctcgacg acctcgccgc gcgcctgggc cagcacgccc tcgagagtga
       61 ggttctgcga cacgtcgacg cggtaaccgt caagcacgcg ctcgagcgga tcgcgcgccg
      121 agtagcgcac gggcttgatg gcgccgccgc cgaagtcctg cagcaccagg ctctggagca
      181 tgtcatccat ctcctcgcgc ggaacctgca ggttcatgac ctggttgcct acgacggtgc
      241 cactgtgctc gaagtagccg acgccgttgg tgaagaggac gacgcgggtc accggcaggt
      301 ctgcctggtc ggtgccctgc gcggacgcga gagcggtcat cattaccccc agtgccatgg
      361 cggacaggag ccgtaggctc cgggcgggac gcttagtagc catggtcgga tgctagcgca
      421 cgcttggccc gctttcggtt gagattccgt caaccgtccg ctcctgcgcc gctcatcctc
      481 gtcacacctt cggagcggcg cgctccccac ccacgtacac cgccaggggc tcaagccccg
      541 gcgagaagag cgcgaagtcg gcgtccttgc cgacctcgag gctgccctta tgctcggccg
      601 cgccgaccgc cctggccggg ttgagcgaag ccatgcgcac gacctccacc agcggcaggt
      661 cgacctcaaa gtgcatgacc tggagcatgc ggtcgaggag ggtagtgcta ccggcgaacg
      721 ctgtgaggtc cttcagcatc gccacgccgc cgctgacgac gatgtcgaga ctgcccagct
      781 tcagctcggt cccctcgggc atgccggcgc cgtgcgttgc gtcgctgacg atgcagaggc
      841 gctcaggccc gatggccttg tacgcgaggc gcaggagcgt cgtcgggagg tggcggtggt
      901 cggctatcac ctcgaccgtc aggccgtcga acgcgagcga ggcctcgagc aggcccggct
      961 tgcgccatgg gccctcccgc acggtcgtcg actgggcgct gaacaggtgg attacgtgcg
     1021 tgagaccgcg ctccatggcg gccgcgaggt gctcgtctcg cgcgctcgag tggccggcgg
     1081 ccggcacgat gccgagcgcg cgtagtctcg aggccagttc caacgccccc ggcagctccg
     1141 gcgcgaagct catcagcctg atagcgtccg cgtaggccag gagctcgtct accgtgccgt
     1201 cgtccgggtt ccggatgtgc gccgggtcct gggcgccgga ctgcgcaagc gcgaagtagg
     1261 gaccttccac gtgcatgccg agtacctgag cgtgcgcggg cgcttcgcct ccgcgcgccg
     1321 ccccgccggc ctgcgcgccc agccacttgc gcccctgcgc cagcgcggca gtgagctcgg
     1381 ggatgctggc ggtcaacgtc gtcggcagga cgctggtgac gccgcggccc agttgcgtcg
     1441 cgagcaccgt tccccacgcc gcgtcgtccg cctcgttgaa caggtgaccg ccggcgccgt
     1501 gcgtgtggat gtcgaccagg ccgggtagga cgtaagcgcc gcgcgcgtcc accgtccgcg
     1561 cgtccaactg gtcgcctgtc agctccgact cgggcgctat gcccgcgatg acgccgtccg
     1621 ctacgagcag gaccgtgtcc ggcgccaggc ggtcggggag gacgagggtg ccgccacgga
     1681 ccaggaggcg ttcgcgggct ctcggcgggc gcgctccagc gtccgtgcca gagcgtgtgt
     1741 ccgcgccgtg gtcgtggcct ggccgtgcgc cgttccgcgc gagcggactc acctggctgc
     1801 cgctcgtagg gtgatcatgt aagacatctc gccgtcccct ccacgtctgg aatccgtcag
     1861 ggagccatcg tacgtcgtgg cctggcggtc cagcgagtcg aaggagaggg tgaagagcgc
     1921 gtcgtcttcc tccgcgccac ctgcgggcac cacacgcgcg gagccggtca ggcgtggtgg
     1981 cgggatcgag gcgggaccga gccgatagtc gtgaaagcgg ctggtccaca cgtggccgta
     2041 gatcgggagg ggcgctgcgc caggtaggtc gggcaggaag gtgccctgga actcgtacga
     2101 gctgtgctcg gggtccgagc gcatggcggt tgccgtcacg ttcaaggtgg cacgggtgag
     2161 ctcggcgagg ggcagggcgg tgtcgcccgg ctcttcgcta agagagacga cgtggacgcg
     2221 cccgtccgcg ccgaccgctg t
//
LOCUS       DAOQYE010000305         2231 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_55393_length_2231_cov_2.557904, whole genome shotgun sequence.
ACCESSION   DAOQYE010000305 DAOQYE010000000
VERSION     DAOQYE010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55393_length_2231_cov_2.557904"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..803
                     /locus_tag="PKN52_05830"
     CDS             <1..803
                     /locus_tag="PKN52_05830"
                     /inference="COORDINATES: protein motif:HMM:NF014663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99499.1"
                     /translation="LLLALHLAVGAPLKQASIEFPVPHATLRAERATLAAFGGAATAA
                     IHGRELAPGVPFEWRAGEELILRPAAAGQRQRAGVPILSVAGGLAPLGGHAGHPLLAE
                     GGSTDVRGRVGGFGRWLRPGDVLTRAARPAPPAARAPGAALGHYPVELGLHLYPGAHG
                     GAAGFARLLSRTFVVGERSRMGVRLEPLGGGDALDLGVTGAEASRGMPLGAVQLPPDG
                     RPLVLLADRGRTGGYPVLGAVARRDLAGLAQARAGTVVTFRAPRERGA"
     gene            complement(741..2213)
                     /locus_tag="PKN52_05835"
     CDS             complement(741..2213)
                     /locus_tag="PKN52_05835"
                     /inference="COORDINATES: protein motif:HMM:NF003715.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HNQ99500.1"
                     /translation="MHRPLIALALILFYGGLDTRMTMFKAAIVPASVLATVGTVLTML
                     FVGLSAYALTSLDLLTSMLLGAVVSSTDAAATFSMLKGRGLPKRLRGTLETESGTNDP
                     AAVLLTYSLAEAITRGGAVNYLALVGDIVLELALGVALGVAFGWLLKRLINKAALGNI
                     GLYPMLALSGALLAFALTGLLHGNGYMAIYLVGLILSHNRLSHRLAIVNFMDGTAWGA
                     QLVMFLLMGLLVNPDQLPAIIGVALLITAACMLIARPVAVGVSLWLVRMVTRGRIAFG
                     IREQLLITWAGLKGAVPIILAMVPLLDRVPGADDIFNVVFVVVIVGTLLQGFTISPLA
                     KALGLSKDEPPPALLRLELGGDAPDGAAVVDVYLAEDNRAIGKRIDELSLPPSVVIAA
                     VLRGKQMVTPRGSTVFRNGDHVYLIGSRMHTESIPTAFGRKWHGVGASPRTANAGADP
                     QSVHSTSAELGSAGPFPDGGSGSAFPGGAEGHDGSGSSLG"
BASE COUNT          350 a          740 c          832 g          309 t
ORIGIN      
        1 ggctcctgct cgcgctgcac ctcgccgtcg gcgctccgct caagcaagcg agcatcgagt
       61 tcccggtccc gcacgcgacg ctgcgcgccg agcgcgccac gctggcagcg ttcggagggg
      121 cggcgacggc cgcgatacat ggtcgggagc tggcgcccgg ggtgccgttc gagtggcgcg
      181 cgggggagga gctgatcctg cgaccggcag cggcaggtca acgccagcgc gccggcgtgc
      241 ccatcctcag tgtggcgggc ggcctcgcgc cgctcggcgg ccacgccggg cacccgctgc
      301 tcgccgaggg agggagcacg gacgtgcgcg gccgcgtcgg ggggttcggg aggtggctgc
      361 ggccaggcga cgtgctgacg cgcgcggcgc gaccggcacc gccggcggcg cgggcgccgg
      421 gggcggccct aggccactac cctgtcgaac tcggcctcca cctctaccct ggggcgcacg
      481 gaggcgccgc tggtttcgca cggctgctca gccggacctt cgtcgtcggc gagcggagcc
      541 ggatgggcgt acgcctcgag ccgttgggcg gtggcgacgc cctggacctt ggggtgaccg
      601 gcgccgaggc gtcgcgcggc atgccgcttg gcgccgtcca actgccgccg gacggcaggc
      661 cgctcgtcct tctggccgac cgcggccgca caggcggcta cccggtcctc ggcgctgtgg
      721 cgagacgcga tctggccggg ctagcccaag ctcgagccgg aaccgtcgtg accttccgcg
      781 cccccaggga acgcggagcc tgagccgccg tccgggaagg gtcctgccga tccgagttcg
      841 gcggacgtgg agtggaccga ctggggatcg gcgccggcgt tagctgtgcg aggcgacgcc
      901 cccaccccgt gccacttgcg tccgaacgcc gtggggatgg actcggtatg catgcgcgaa
      961 ccgatcaggt agacgtggtc gccgttcctg aacaccgtgc tgccgcgcgg tgtcaccatc
     1021 tgcttgcccc gcagcactgc ggctatcact accgagggcg gcaacgagag ctcgtctatg
     1081 cgcttgccga tggcgcggtt gtcctctgca aggtagacgt ccaccacggc ggcgccgtcc
     1141 ggcgcgtcgc cgccaagctc gagcctcagc agcgccggtg gcggttcgtc cttcgagagc
     1201 ccgagcgcct tggccaacgg cgagatggtg aagccctgca ggagcgtccc cacgatcacc
     1261 accacgaaca cgacgttgaa gatgtcgtcg gcgccaggca cacggtccag gagcggcacc
     1321 atggcaagga tgatcggcac cgcgcccttg agaccggccc aggtgatgag aagctgctcg
     1381 cggatcccga acgcgatgcg tccacgcgtg accattcgca cgagccagag agaaaccccg
     1441 acggccacgg ggcgggcgat gagcatgcaa gccgcggtga tgagcaaggc tactccgatt
     1501 atggcgggta gctggtccgg gttcacgagc aggcccatca ggaggaacat gacgagttgc
     1561 gctccccacg cggtgccgtc catgaagttg acgatagcga gccggtgcga gaggcggttg
     1621 tggctcagta tgaggccgac gaggtagatg gccatataac cgttgccgtg caggaggccc
     1681 gtcaacgcga acgcgaggag tgcgccggac agcgccagca ttgggtacag gccgatgttg
     1741 ccgagcgccg ccttgttgat gaggcgcttg aggagccaac cgaacgcgac gccgagcgcg
     1801 acgccgagcg ccagctcgag gacgatgtct ccgaccagcg caaggtagtt gacggctcca
     1861 ccgcgcgtga tggcctcggc gagcgagtaa gttagcagga ccgcggccgg atcgttcgtg
     1921 ccagactcgg tctccagggt gccgcgcagt cgcttaggga ggccgcggcc cttgagcatg
     1981 gagaacgtgg ccgccgcgtc ggtgctcgac acgaccgcgc cgagcagcat gctggtcagc
     2041 aggtcgaggc tggtgagtgc gtaggcgctc aggccgacga agagcatggt gagcaccgtg
     2101 ccgacggtgg cgagtaccga ggcgggcacg atcgccgcct tgaacatggt catgcgcgtg
     2161 tccaggccgc cgtagaagag gatcagggcg agggcgatca gcgggcgatg catcgagcct
     2221 ccacgatcgg c
//
LOCUS       DAOQYE010000306         2228 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_55488_length_2228_cov_3.717441, whole genome shotgun sequence.
ACCESSION   DAOQYE010000306 DAOQYE010000000
VERSION     DAOQYE010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55488_length_2228_cov_3.717441"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            157..846
                     /locus_tag="PKN52_05840"
     CDS             157..846
                     /locus_tag="PKN52_05840"
                     /inference="COORDINATES: protein motif:HMM:NF012709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HNQ99501.1"
                     /translation="MVGFVILAALLLAGGVGVVTLRQPVHAALALVGTLLALAVTYVT
                     LDAHFLAAVQVIVYAGAIMVLFLFVIMLLNVGGERTADRLKWLKPAAWGSAAVTAAGL
                     ATVYFLQRQPLPGASVIDGALGGGGADRIGEVLFTDYLLAFQLVGVLLLTGVVAAVSL
                     VQRAAPETRPQRERTSRVAPHGPDTEAGATPHLVGALGAGAAPSTGVASGAGATQGAA
                     AAGDELAHGDH"
     gene            846..1166
                     /gene="nuoK"
                     /locus_tag="PKN52_05845"
     CDS             846..1166
                     /gene="nuoK"
                     /locus_tag="PKN52_05845"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HNQ99502.1"
                     /translation="MVGTEYYVAISAVLFAIGAVGVLTRRSAIMLFLSVELMLNAANL
                     AMVAFARSWSASGATTALNGQAAVFIVLAVAAAEVAVGLGILVAIFRNRVSTNVDELS
                     EVRA"
     gene            1163..>2228
                     /locus_tag="PKN52_05850"
     CDS             1163..>2228
                     /locus_tag="PKN52_05850"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HNQ99503.1"
                     /translation="MTDLAHWAALAPLLALAGALVNGLFGRNLKEPAPGIIASVAVAA
                     GFVLSLIAFFGVAGSGEAVRVGFGDFLRAGDFTLSLGFHLDQLSVLMMLIITGVGLLI
                     HVYSIGYMHGDPGYSRYFAQLNLFVASMLVLVMADSFVLMFIGWEGVGVCSFLLIGFW
                     YHDLANASAGRKAFIVNRVGDVGFLLAMFLTFKVFGSLDIQTVSAMAPTMLYGSAVLT
                     GIGLLYLLAAAGKSAQLPLHVWLPDAMAGPTPVSALIHAATMVTAGVYLIARTSAVYA
                     VAPAALATVAWVGVLTALVAAIAAVGQTDIKRILAYSTISQVGFMIAAVGAGAYWAGI
                     FHVLTHAFFKALLFLGAGSVI"
BASE COUNT          283 a          848 c          709 g          388 t
ORIGIN      
        1 tccgctacca ggacttcgtc taccgcaagg aagacatgct cgtcggcacc aaggggagca
       61 agtggcagcg tcgcgaggcc gacgccaagg gcaaggacgt gaagctaggg ttcgaggccg
      121 gtccccggtc cgagctggaa ggcgtcgact actgacatgg tcggtttcgt catcctagcc
      181 gcgctcctgc tggccggcgg cgtcggcgtc gtcacgctgc gccagcccgt ccacgccgcg
      241 ctcgcgctcg tcggcacgct cctggcgttg gccgtcacgt acgtgacact cgacgcgcac
      301 ttcctcgccg ccgtccaggt catcgtctac gcaggcgcca tcatggtcct cttcctcttc
      361 gtgatcatgc tcctcaacgt gggcggcgag cggacagccg acaggctcaa gtggctcaag
      421 ccagccgcgt ggggcagcgc ggccgtcacc gccgccggtc tcgcgaccgt ctacttcctc
      481 cagcggcaac ccctccccgg cgcctccgtc atcgacgggg cgttgggcgg gggcggcgcc
      541 gaccggatcg gcgaggtcct cttcacggac tacctgctcg ccttccaact cgtcggcgtg
      601 ctgttgctca ccggcgtggt cgcggccgtg agcctcgtgc agcgcgccgc gccggagacg
      661 cggccgcagc gcgagcggac gtcccgcgtc gcgccgcacg gacccgacac cgaggcgggc
      721 gcgacgccgc acctcgtcgg cgctctcggg gccggcgcgg cgcctagcac cggcgttgct
      781 tccggtgcgg gcgccacgca aggcgccgcc gccgcgggcg acgagctcgc gcacggagac
      841 cactgatggt aggcaccgag tactacgtcg ccatcagcgc cgttctcttc gccatcggcg
      901 ccgtcggggt cctgacgcgc cggagcgcca tcatgctctt cctgtcggtc gagctgatgc
      961 tcaacgccgc caacctcgcg atggtcgcct tcgcgcggag ctggagcgcg agcggcgcca
     1021 ccacggcgct caacggtcag gcggccgtgt tcatcgtcct ggccgtcgcg gcggccgagg
     1081 tggccgtcgg actcggcatc ctcgtcgcca tcttccgtaa ccgcgtcagc accaacgtgg
     1141 acgagctgtc ggaggtgcgc gcatgaccga cctagcccat tgggccgccc tcgcgccgct
     1201 gctggcgttg gcaggcgccc tcgtgaacgg cctgttcggc cgcaacctca aggagcccgc
     1261 gccgggcatc atcgccagcg tcgcggtggc ggccggcttc gtcctctcgc tcatcgcctt
     1321 cttcggcgtg gccggcagcg gcgaagcggt gcgcgtcggc ttcggcgact tcctgagggc
     1381 cggagacttc accctctccc tcggcttcca cctcgatcag ctgagcgtcc tgatgatgct
     1441 gatcatcacg ggcgtcggct tactcatcca cgtctactcg atcgggtaca tgcacggcga
     1501 tccgggctac tcgcgctact tcgcgcagct caacctgttc gtcgcctcca tgctcgtgct
     1561 cgtcatggcc gactcgttcg tgctcatgtt catcggctgg gaaggcgtcg gggtctgctc
     1621 cttcctcctc atcgggttct ggtatcacga cctcgccaac gcgagcgccg gccgcaaggc
     1681 gttcatcgtc aaccgcgtcg gcgacgtcgg cttcctgctc gccatgttcc tgaccttcaa
     1741 ggtcttcggc agcctcgaca tccagacggt cagcgccatg gcgccgacca tgctctacgg
     1801 ctccgccgtg ctgaccggca tcgggctcct ctacctgctg gccgcggccg gcaagtcggc
     1861 gcaactgccg ctgcacgtct ggctgccaga cgccatggcc ggccccacgc cggtctcggc
     1921 gctcatccac gccgcgacca tggtcacggc cggcgtctac ctcatcgccc gcacgagcgc
     1981 cgtctacgcg gtcgcgccgg ccgccctcgc caccgtggcg tgggtcggcg tcctcacggc
     2041 cctcgtcgcg gccatcgcag ccgtcggtca gaccgacatc aagcgcatcc tcgcctactc
     2101 caccatctcg caggtcggct tcatgatcgc ggccgtcggg gccggagcct actgggccgg
     2161 catcttccac gtcctcacgc acgccttctt caaggccctg ctcttcctag gcgccggctc
     2221 cgtcatcc
//
LOCUS       DAOQYE010000307         2228 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_55509_length_2228_cov_2.495168, whole genome shotgun sequence.
ACCESSION   DAOQYE010000307 DAOQYE010000000
VERSION     DAOQYE010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55509_length_2228_cov_2.495168"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="PKN52_05855"
     CDS             complement(<1..226)
                     /locus_tag="PKN52_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HNQ99504.1"
                     /translation="MSALTMVQSIARTLDEEMARDERVVVVGEDVGKRGGVFLATEKL
                     FDKYGPDRVIDSPLSEAAIIGAAVGMAAHGL"
     gene            complement(243..1340)
                     /locus_tag="PKN52_05860"
     CDS             complement(243..1340)
                     /locus_tag="PKN52_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HNQ99505.1"
                     /translation="MILESQRFQPFTAEPIAVISESGEWIAPFELDLGPDQLRRMYQD
                     MLMGRAVDERLGRLQRQGKVSFVAPSGGHEGAQVGIAHALRAGHDWLFPYYRDVALPL
                     AMGLPLVEFVAQAMGSAADPSRARQMPYHVSSKALNLFSAASPIASHIPPAAGCAISM
                     KLRGTDQVTVCTFGDGATSEGDWHAGVNFAGAQGAPIVFACQNNRYAISVGFEKQTGA
                     DSIYTKAHAYGMPGYFVDGMDVLASYYVMREAVQRARDGFGPALVELLVYRYGGHSSA
                     DDDSRYRPRAEVEAWRKRDPIARLRRFLTRRGLWDDEAQKEADARIAAEFDEAVAQAQ
                     AAGDVPVEAMFEDVFAEVPERLARQRAELLG"
     gene            1797..>2228
                     /locus_tag="PKN52_05865"
     CDS             1797..>2228
                     /locus_tag="PKN52_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99506.1"
                     /translation="MRESKVATLTRILLAAATLTALLLLAAYVSSKAWLWLLCAAVIF
                     LFVYARNGTYATLMLGALLVGAAVGSLLEVALRWQGAFLMSLGAAAITVEALEERPGN
                     WAFAFGVAFVGIGTVVALASAGMRGYLAFVLLAALTAAVLAL"
BASE COUNT          327 a          745 c          794 g          362 t
ORIGIN      
        1 gcagcccgtg cgccgccata ccgacggccg cgccgatgat ggcggcctcc gagagcggcg
       61 agtcgatgac acggtcgggg ccgtacttgt cgaagagctt ctcggtggcg aggaacactc
      121 cgccgcgctt gccgacgtcc tcgccgacca ccacgacgcg ctcgtcacgc gccatctcct
      181 cgtcgagggt gcgcgcgatg ctctgcacca tcgtcaacgc gctcatgcgc cgccacgccc
      241 gctcatccga ggagctccgc acgctgcctg gccagacgct cggggacctc ggcgaagacg
      301 tcctcgaaca tcgcctctac gggcacgtcg ccggcggctt gggcctgggc gacagcctcg
      361 tcgaactcgg cggcgatcct ggcatcggct tccttctgcg cctcgtcgtc ccagaggccc
      421 ctacgggtga ggaagcgccg cagccgggcg atcgggtcgc gcttgcgcca ggcctcgacc
      481 tcggcgcgcg ggcggtagcg ggagtcgtcg tcggcggacg agtgcccgcc gtagcggtac
      541 acgaggagct cgacgagggc ggggccgaag ccgtcgcggg cgcgctggac tgcctcgcgc
      601 atgacgtagt acgacgcgag cacgtccatc ccgtcgacga aatagccggg catgccgtag
      661 gcgtgagcct tggtgtagat gctgtcggcg cccgtctgct tctcgaagcc cacgctgatg
      721 gcataacggt tgttctggca ggcgaagacg atgggcgcgc cctgggcgcc ggcgaagttg
      781 acgccagcgt gccagtcgcc ctcgctcgtg gcgccgtcgc cgaacgtgca gacggtcacc
      841 tggtcggtgc cgcgcaactt catgcttatc gcgcagcccg cggccggtgg gatgtgggag
      901 gcgatcggcg aggcggcaga gaagaggttg agcgccttgg aggacacgtg gtagggcatc
      961 tgcctggcgc ggctggggtc ggcggccgag cccatggcct gcgcaacgaa ctcgaccagc
     1021 ggaaggccca tggcgagcgg cagggcgacg tcgcggtagt acgggaacag ccagtcgtga
     1081 ccggcgcgca gcgcgtgagc gatgcccacc tgggcgcctt cgtgcccacc ggaaggggcc
     1141 acgaagctga ccttgccttg gcgctggagg cgcccgaggc gctcgtcgac cgcgcgaccc
     1201 atgagcatgt cctggtacat gcggcgcaac tggtccggac cgaggtcgag ctcgaacggg
     1261 gcgatccatt caccggactc gcttatgacg gcgatgggtt cggccgtgaa cggctggaag
     1321 cgttgcgact cgaggatcat gctggcctcc cttcgttcac atggtgcgtt atcggcatgc
     1381 aagcttgtcg tggaacacgt tgggtggatg acagggcaac gccccggcgg ttacgcgccg
     1441 gggcgttcgg gttactaggt cggcatgcgc cgatcgacgc tagcaccgag acaggtagcg
     1501 accggctcga accggacggg tcgacggcac cgacgacgcg agcgtgcgga agggcacgga
     1561 ccaaatggcc cgacgaaccc cggcacgggg gccgtaccgc gcgctgctgt tcggatgtgg
     1621 ctcgactctt aaccatggct ggtcggcaag ctcggccgga tcgcaggctg ctcggcaccg
     1681 ttcagcggac cggcaggaac gccccggtcg tcccttcgag cgttgggcgc aggatagcat
     1741 ctgaccgcgc accgcgccgt agcctattcc ccaaccccgg cgcggtggac ggtggtatga
     1801 gggagtcgaa agtggcgacc ctgacccgca tcctcctggc ggcggccacg ctgaccgctc
     1861 ttctactact tgccgcgtac gtttccagca aggcgtggct ttggctccta tgcgcagccg
     1921 tgatcttcct gttcgtctat gcccgcaacg ggacgtacgc cacgctcatg cttggcgccc
     1981 tgctcgtcgg cgccgcggtc gggagccttc tcgaggtggc cctgcgatgg caaggcgctt
     2041 tcctcatgag cctaggcgcg gccgcgatca cggtcgaggc cttagaggag cgcccaggca
     2101 actgggcgtt cgcgttcggc gtcgccttcg tcggcatcgg caccgtcgtc gccttggcga
     2161 gcgccggcat gcgtgggtac ctggcgttcg ttctcctcgc cgcgctgacc gcggcggtcc
     2221 tggcgctg
//
LOCUS       DAOQYE010000308         2225 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_55618_length_2225_cov_2.532719, whole genome shotgun sequence.
ACCESSION   DAOQYE010000308 DAOQYE010000000
VERSION     DAOQYE010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55618_length_2225_cov_2.532719"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            198..572
                     /gene="rsfS"
                     /locus_tag="PKN52_05870"
     CDS             198..572
                     /gene="rsfS"
                     /locus_tag="PKN52_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HNQ99507.1"
                     /translation="MTKEQHQDVVGLPDGVQTIVDALDDKRAIDIAVLDLRTVSETLD
                     CFIVASGESSLQLRALEEEVLARCKAQGRLARGVEGPSERWVLIDYGPVVVHLMSPEA
                     RDFYDLEGLWADAERVEVTPSR"
     gene            569..928
                     /locus_tag="PKN52_05875"
     CDS             569..928
                     /locus_tag="PKN52_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HNQ99508.1"
                     /translation="MSRARPELHWAEAAARVHLEKLGYRHLASNYRLRGGELDLVMED
                     GHCLVVVEVKQRKDARFGHPLEAIDGGKLRRLRRAALHYAKAAGGRPGAALRLDAVVL
                     LGDEGDYRLEHLRDVGW"
     gene            925..1575
                     /locus_tag="PKN52_05880"
     CDS             925..1575
                     /locus_tag="PKN52_05880"
                     /inference="COORDINATES: protein motif:HMM:NF012900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HNQ99509.1"
                     /translation="MSAPDTPARNAARRPVRVKVCGITSVADAVVAEAAGADAVGMIF
                     APGSKRRVTPETAADICSALGPFVARIGVFVDADVAEVVGLARGLRLTGVQLHGGESA
                     AYAREVAKATRVVRALPYAPGLVPADVADYGADAVLLDAATPGSGLAFAWEAAAPAWR
                     AWPRLVLAGGLTPDNVSAAIGALRPYAVDVASGVETRPGVKDPEAVRAFVSAAKDA"
BASE COUNT          324 a          777 c          797 g          327 t
ORIGIN      
        1 gactactacg cggccatgct caacgtcggc aagcagcaga tcgatcggtt cgcgtccgtc
       61 cagcggcgtt ccgtccagct cgagctcgtg ctcgggccgg acgccatgac caggaccgcg
      121 gcgaggacgg acctcaccgg agccagcctc gcggttggcg actgacaagc gcaaggcggg
      181 cagccgccgg agggaacttg actaaggaac agcatcagga cgtggtcggc ctgcccgacg
      241 gagtgcagac gatagtggac gcgctcgacg acaagcgggc gatcgacatc gccgtcctcg
      301 acctacggac ggtgtcggag acgctcgact gcttcatcgt ggcgagcggc gagtccagcc
      361 tgcaactcag agccctggaa gaagaggtgc tcgcgcgttg caaggcgcag ggccggctgg
      421 cgcgcggcgt cgagggcccg tcggagcgct gggtgctgat cgactacggc cccgtcgtcg
      481 tccatctgat gagccctgag gcccgcgact tctacgacct cgagggcctg tgggccgacg
      541 ccgagcgcgt ggaagtgacg ccgagcaggt gagccgagcg cgccccgagc tgcattgggc
      601 cgaggcggcg gcgcgcgttc acctcgagaa gctcggctac cgccacctcg ccagcaacta
      661 ccgcttgcgc ggcggcgagc tcgatctggt gatggaggac ggccactgtc tcgtcgtggt
      721 cgaggtgaag cagcgcaagg acgcgcgctt cggccacccg ctcgaggcca tcgacggggg
      781 caagctccga cgcctcaggc gcgccgccct gcactacgcc aaggcggcgg gcggtaggcc
      841 gggcgccgct ctgcgcctcg acgccgtcgt cctcctgggc gacgagggcg actaccggct
      901 tgagcacctg cgggacgtgg gctggtgagc gcgcctgaca ccccggctcg taacgccgcg
      961 cgccgacccg tgcgcgtgaa ggtctgtggc ataacatccg tcgccgacgc cgttgtggcc
     1021 gaggcggccg gggcggacgc cgtcggcatg atcttcgctc ccggctccaa gcgccgggtg
     1081 acgcccgaga cggctgccga catctgctcg gcgctcggtc cgttcgtcgc ccgcatcggc
     1141 gtgttcgtcg acgcggacgt cgcggaggtg gtcggtctcg cgcggggtct ccggctgacg
     1201 ggggtccaac tccacggcgg cgagtcggcc gcttacgccc gcgaggtcgc caaggcgacc
     1261 agggtggtgc gcgcgctgcc gtacgccccg gggctcgtac cggccgacgt ggccgactac
     1321 ggcgcggacg ccgtcctcct cgatgccgcc acgccgggct ccggcctggc gttcgcctgg
     1381 gaggcggccg cgccggcctg gcgcgcttgg cccaggctgg tgctcgcggg agggctgacc
     1441 ccggacaacg tctcggcggc catcggcgcc cttcgaccgt acgcggtcga cgtggcgagc
     1501 ggcgtggaga cgcgccccgg cgtgaaggac cccgaggccg tgcgggcctt cgtgagcgcc
     1561 gccaaggatg catagtcggc cctccaggta tgcagaggct gggggccgct ctcgacgtca
     1621 tcctcggcgg ccggcgcgtc ggccctccgg gcatgcagag gttggaccgc tggcacccgg
     1681 ctttgccccc ggccccgtgt tcatcggccc aacgtagtta tccacaaata acgcccgttc
     1741 tccacaggct tgtggacaag cgccgggctc cggaccctac tccttccagc cgtccgatga
     1801 gcacggcaca tttgcagcgt tagcgtccct gacgacatct ctcgcgagag cgcgacaggc
     1861 gcgccgcgat accagtcggc gccggcgctt gtcaagccct cggcgttatc cacagacctt
     1921 gcacctattg ccacatgccg gatatggccg ccgctgcggg ccatcttggt cggcccgacc
     1981 gtgctcgcgc cgacctccgg ccgtccacgg cgcggcggtg ccgccgggcg cctcgacagg
     2041 ttgtacgcta gcttcgagca accccgagag ccatcgtcct gggaggcgaa cgtgaagacg
     2101 gtaacgatca gcggagccag gcgcttctca ctcgagtccg tgcaacgccg gaccttggtg
     2161 gaggaggctg agctgctcgt ggagctcatc tgcttcgagg ccggacaagg cgatgagccg
     2221 agcag
//
LOCUS       DAOQYE010000309         2224 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_55642_length_2224_cov_3.067773, whole genome shotgun sequence.
ACCESSION   DAOQYE010000309 DAOQYE010000000
VERSION     DAOQYE010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55642_length_2224_cov_3.067773"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1477)
                     /locus_tag="PKN52_05885"
     CDS             complement(<1..1477)
                     /locus_tag="PKN52_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99510.1"
                     /translation="MKKRPTKQRRLLRLALAIMALLTACGQPATPTRTRPAVIPETTK
                     VTDEETRTALTDYDPVSGTMRFATNTGFLADLRPDDVLVSVPSTAAPNGYLRKVKTIR
                     QEAGEVVLETTQANLTDAISQGDLDVHYDLEPADLQSATALVEGLQVGLAPQDAVDLG
                     DGYSFRAQFNETVLDIDDGDVTVQVKVSGDLYFNAGYNIGIGIDNINPLAGRLTPEVD
                     RFEFWIGFEQAARLHVWGEANAKLSKEKKVAEYRFTPQCFLILVVPICFVPTVYVFVG
                     ASGEVHLNFDYSVEQTAAAKIGAKWVDDKGWSTFDPAPAFDTSFDQNFAVAAGLGLEA
                     HTKAEASLMIYGVAGPTLGAQMGVKLDAAVPRDPFWILTGSLEGYYGFIVDIPVVGRV
                     ADSSGTIFQRSKEFGRSSNSPPTIVVKSPTNRVDLGKEVNLGFLTNGGSCDDFYGIYC
                     VIDPETGKTPFTLTSDLDGALPAAMYTFQTPGLRTITIRATD"
     gene            complement(1693..>2224)
                     /locus_tag="PKN52_05890"
     CDS             complement(1693..>2224)
                     /locus_tag="PKN52_05890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99511.1"
                     /translation="VNGRSDVAKLDLIDELYPGKDEEKASSSLKEVVRGLRATFGAGL
                     VATTPGGYSLGACTSDLEAFLAEPDGALWRGAYLDGLELEGGVRDTVHMALARHAQEL
                     LPYRPKEAARLAGILVEAEPYQLAYLRLQLEALRVAGNHRSLGRHYRAAVIRMAELGS
                     TLPDSWQAFLDEPAHR"
BASE COUNT          352 a          707 c          751 g          414 t
ORIGIN      
        1 tatcggtggc gcggatggtg atggtgcgca gacccggggt ctggaaggtg tacatagcgg
       61 ccggcagcgc cccgtccagg tccgaggtca gcgtgaacgg cgttttgccc gtctcgggat
      121 cgatgacaca gtagatcccg tagaagtcgt cacaacttcc gccgttcgtg aggaacccga
      181 ggttcacctc cttgcccagg tcgacgcggt tcgtcggcga cttcaccacg atcgtgggcg
      241 gtgagttgga cgaccgcccg aactccttgc tgcgctggaa gatggtgccg ctgctgtcgg
      301 ccacgcggcc cacgaccggg atgtcgacga tgaagccgta gtacccttcc aacgagccgg
      361 tgaggatcca gaaggggtcg cgcgggacgg ctgcgtcgag cttcacgccc atctgcgcgc
      421 ccagcgtggg accggcgacg ccgtagatca tcagcgacgc ttcggccttg gtgtgcgcct
      481 cgagcccgag cccggcggcc acggcgaagt tctggtcgaa actcgtgtcg aacgccgggg
      541 cgggatcgaa cgtgctccaa cccttgtcgt cgacccactt ggcgccgatc ttggccgcag
      601 ccgtctgctc gacgctgtag tcgaagttca gatgaacctc gccgctggcg ccgacgaaca
      661 cgtagaccgt tggcacgaag cagatcggca cgaccaggat caagaaacac tgaggcgtga
      721 agcggtactc agcgaccttc ttctccttgc tcagcttggc gttggcttct ccccagacgt
      781 gcaacctggc agcctgctcg aaaccgatcc agaactcgaa gcggtccacc tcaggagtca
      841 gacgccctgc tagggggttg atgttgtcga tcccgatgcc gatgttgtaa ccggcgttga
      901 agtacaggtc accgctcacc ttcacctgca cggtgacgtc gccgtcgtcg atgtcgagca
      961 ccgtctcgtt gaactgtgcc ctgaaggagt acccgtcgcc gaggtcgacg gcgtcttgcg
     1021 gcgccaagcc gacctggagc ccctctacca acgccgtggc ggattgcagg tcggccggct
     1081 cgaggtcgta gtgcacatcg aggtcgccct gagagatggc gtcggtgagg ttggcctggg
     1141 tggtctcgag caccacctcg cctgcctcct gacggatcgt cttgaccttg cgcaagtacc
     1201 cgttgggcgc cgccgtcgac ggtacggaga cgagcacgtc atcgggccgt aggtccgcca
     1261 ggaagccggt gttcgtcgcg aagcgcatgg tgccggagac ggggtcgtaa tccgtgaggg
     1321 cggtgcgcgt ctcttcgtcg gtgaccttgg tggtctcggg gatcaccgca ggcctggtgc
     1381 gagtcggcgt ggccggctga ccgcacgccg tgagtagcgc catgatcgcc agagccagcc
     1441 gtagaaggcg tcgctgcttc gttggtcgct tcttcatcgc ctttctcctt caccacgagt
     1501 tccgtgcacc ctgtgaggcc ggcccgccgt tacgccgcgt gatcccaagc tctcgtcaag
     1561 cggcatgtaa gggccggcag ggcacgggcc gaagtctgag gtagaggcat cctcatgctg
     1621 tcagcctagt gcggtccgag gaaaggtgcc gtcaggtttt ctagcgtttg cgccggtgcg
     1681 atacgcggtg cgtcagcggt gcgccggctc gtcgaggaag gcctgccagc tgtccggcag
     1741 cgtgctccca agctcagcca tgcggatgac ggcggcgcgg tagtggcgcc caaggcttcg
     1801 gtggttgcct gccacgcgca gggcctctaa ctggagcctc aggtaggcga gctggtacgg
     1861 ctccgcttcg acgaggatcc cggcaagtcg cgccgcctct ttgggtctgt atgggagcag
     1921 ctcctgggcg tgccgagcga gcgccatatg gacggtgtct cgcacgccgc cctccagttc
     1981 caggccgtcg aggtacgcgc cccgccacag tgccccatcg ggttcggcga ggaaggcctc
     2041 gaggtccgag gtacatgcgc ctagggagta cccgcccggc gtggtggcga cgagcccggc
     2101 accgaaggtg gcgcgcaagc ctcgcacgac ctccttgagg ctcgagctcg ccttctcctc
     2161 atctttgccg gggtagagct cgtcgatgag atcgagcttg gcgacgtcgg atcgtccgtt
     2221 cacc
//
LOCUS       DAOQYE010000310         2215 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_55986_length_2215_cov_3.109722, whole genome shotgun sequence.
ACCESSION   DAOQYE010000310 DAOQYE010000000
VERSION     DAOQYE010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55986_length_2215_cov_3.109722"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1167)
                     /locus_tag="PKN52_05895"
     CDS             complement(<1..1167)
                     /locus_tag="PKN52_05895"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HNQ99512.1"
                     /translation="MPGIAMIGAQWGDEGKGKVVDALAAEADFVVRYSGGANAGHTVV
                     VGDEVFKLHHLPCGTLHDRPTSVLGGGMVIDPWTFVEELDALSTRRDPGRVLVSTEAH
                     LVLPHHKKNDEGGGFVGTTGRGIGPAYSDKARRIGVRAGDLLDDALMRERLARLLEAK
                     PNSTARVGWSSVAYAMDELAEVRERLAPMVADTGELVRAALKRGERVLFEGGQGTMLD
                     LAYGTYPYVTSSHPSVGGILVGAGVSHKALGAVYGVVKAFTSRVGHGPFPTELTDDAM
                     ALRLRGSGSNQWDEYGTTTGRPRRVGWLDLPQLRYACEINGFDGLVVTKLDVLSGLER
                     VRVAVDHGPDGPVYEDLPGWGELRGLGSREALPRQVLDYLALIEERTGVPVSIFS"
     gene            1318..2034
                     /locus_tag="PKN52_05900"
     CDS             1318..2034
                     /locus_tag="PKN52_05900"
                     /inference="COORDINATES: protein motif:HMM:NF019342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HNQ99513.1"
                     /translation="MLLSTGISFRTTGLLREDSGTGRNYSQAVALAGGLPTMVAVLDT
                     QLVGEYAATHDALLLTGGVDVDPALFGAIPHPDLGSVDERRDAFEIELYHAFRKLGKP
                     VLGICRGHQVINVAEGGTLHQHVPALPEAWQHEQHDLRGSPLHPVRLTPGSRLASAFG
                     TTDVRTNSYHHQAVDVVGVGLKAVAHSGDGLVEAVEERHGSWVLGVQWHPEMAYKEHP
                     EHHAPFRLLLEAVKMVGARA"
BASE COUNT          330 a          794 c          769 g          322 t
ORIGIN      
        1 cgagaagatg ctcaccggca cgcccgtgcg ctcctcgatg agggcgaggt agtcgagcac
       61 ctgccggggc agggcctcac ggctgccgag cccgcgcagc tcgccccagc cgggcaggtc
      121 ctcgtacacg gggccgtccg gcccgtggtc gacggcgacc cgcacgcgtt ccagcccgga
      181 gaggacatcg agcttcgtca ccacgaggcc gtcgaagccg ttgatctcgc acgcgtaccg
      241 gagctgcggc aggtcgagcc agccgacgcg gcgcgggcgg cccgtggtgg tgccgtactc
      301 gtcccactgg ttgctaccgc tcccccgcaa ccggagcgcc atggcgtcgt ccgtgagctc
      361 ggtggggaac ggtccatgac cgacgcggct cgtgaacgcc ttcaccacgc cgtacacggc
      421 gccgagcgcc ttgtggctga cgccggcgcc cacgaggatg ccgccaacac tcggatgact
      481 cgacgtcacg tacgggtacg tgccgtaggc gaggtcgagc atggtgccct gcccaccctc
      541 gaagagcacg cgctcgcccc gcttgagggc ggcgcgcacg agttcgccgg tgtccgcgac
      601 catgggcgcg agacgctcgc gcacctcggc gagctcgtcc atggcgtagg ccacgctcga
      661 ccagccgacg cgggccgtcg agttcggctt cgcctccagg aggcgcgcga ggcgttcgcg
      721 catgagcgcg tcatcgagga ggtcgcccgc cctcacgccg atgcgcctgg ccttgtcgga
      781 gtacgccggg ccgatgccgc ggccggtcgt gccgacgaag ccgccgccct cgtcgttctt
      841 cttgtggtga gggagcacta ggtgagcctc ggtggagacg agcacgcggc cggggtcgcg
      901 gcgcgtactc agggcgtcga gctcctcgac gaacgtccac gggtcgatga ccatgccccc
      961 acccaggact gacgtagggc ggtcgtgcag cgtgccgcaa gggaggtgat ggagcttgaa
     1021 cacctcgtcc cctacgacca ccgtgtgccc ggcgttggca ccgccggagt agcgcaccac
     1081 gaagtcggcc tccgcggcga gcgcgtcgac gaccttcccc ttgccctcgt cgccccactg
     1141 ggcgccgatc atcgcgattc caggcatcgg ccctccacgt tcggcgcgct cgtctcgtga
     1201 agcgtgggcg cgccaagcct cgggcagcct accaggccct ggtcgggagt gaggatggcg
     1261 ctagcgcccg gctcggcgca ggagtactct ggcacgatgt ccgaccatcg accacgcgtg
     1321 ttgctcagca ctgggatcag cttccgcacg acgggcctgc tacgcgagga ctccggcacg
     1381 gggcgcaact actcgcaagc cgtcgcgttg gcgggagggc tgccgacgat ggtggccgtg
     1441 ctcgacaccc agctcgttgg tgagtacgcg gctacgcatg acgccttact cctcacgggc
     1501 ggcgtagacg tcgaccccgc cctcttcggg gcgatcccgc acccggacct tggcagcgtc
     1561 gacgagcgcc gcgacgcgtt cgagatcgag ctctaccacg cgttccgcaa gctcggtaaa
     1621 cccgtgctcg gcatctgccg cggtcaccag gtcatcaacg tggcggaggg tggcacgctg
     1681 caccagcacg tcccggcgct gcccgaggcg tggcagcacg agcagcacga cctccgcggc
     1741 tcaccgctcc accccgtgag gctcacgccc ggcagccggc tggcgagcgc cttcggcacg
     1801 accgacgtac gtacgaacag ctaccaccac caggccgtcg acgtggtcgg cgtcggcctg
     1861 aaggccgtgg cgcactccgg cgacgggctg gtagaggccg tcgaggagcg ccacggctcc
     1921 tgggtcctcg gcgtgcagtg gcacccggag atggcctaca aggagcaccc agagcaccac
     1981 gcgccgttcc gcctcctgct ggaggccgtc aagatggtcg gcgcgcgggc gtagggctcg
     2041 tacggcgcgc gcgtaaggcc ggcagtaacc ggacaaggga cctgcccggg tgccgaggta
     2101 accaccagcc ggcgtcagtc agtccgagcg ggtcgccgcc cgccagtcga cgcgcggcgt
     2161 tatgtccttg acgatggtct cgatccatgc ttttctcgtg atgaacttgc agggc
//
LOCUS       DAOQYE010000311         2209 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_56231_length_2209_cov_2.840297, whole genome shotgun sequence.
ACCESSION   DAOQYE010000311 DAOQYE010000000
VERSION     DAOQYE010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56231_length_2209_cov_2.840297"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            72..971
                     /locus_tag="PKN52_05905"
     CDS             72..971
                     /locus_tag="PKN52_05905"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNQ99514.1"
                     /translation="MSAPGTGRADEAPTTRRLTISGATLEVDDSALADPAAAGKPAII
                     TLHGAPGMGSRRNDWEVYAAFTDRFRVVSYDQRGSGASSDTPPYTHEQFAADTDAVRR
                     TLGLGDKIVIAGGSYGGYLAQEYALRYPEHVSAVVLRDTAAHSGHKGEAMRIALESPY
                     PMDEPELERLFGGMTTSDDDFRALITMIMPLYTTTYDPERDARQVAAMQLHYATHNYA
                     FAHNQQGFDLRGRLGELRVPVLITVGRHDWITPLVASEELHALIPDSELVIFENSGHG
                     PQNEEREAWRAVVRRFLDRVVPA"
     gene            968..>2209
                     /locus_tag="PKN52_05910"
     CDS             968..>2209
                     /locus_tag="PKN52_05910"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HNQ99515.1"
                     /translation="MSGNGRVGPVPIGPAEYAQRARTASERALGGAGADVIVLFDDQY
                     IQYFTGFVFSPTERPIALIVGPDGERALFVPRLELEHAEALADVDRVVAYPEYPGETH
                     PLELLLAETRRLGVGKRAIAVDHDGYPPVMGYAPVRLSDLTDAKVVSLGVALDEQLAL
                     KSEHEVALVRESARWGAHAHRLLQDGTKPGLTEDAVVGAACAAATRAMSASLGGPYRQ
                     RNRWVRGALAIYRGQIGPAGAFPHALAHDATFRVGDTLVTGAAADVYGYLSELERTMF
                     LGEPSAEQRHYFDHMLALQDLCFAAIRPGVAAASVDLAMRAYVDEHSLWPHWRHHVGH
                     GLGQRIHESPFLDLGYEGVLEAGMILSVEPGQKVSQGDVLARSPLESAKTKDITGGLP
                     RVAELFEARRPKDHAIIAEIDG"
BASE COUNT          350 a          802 c          753 g          304 t
ORIGIN      
        1 tacagcgacg acgatatcgg gaaggtcgtt ggcggcaaca tcatgcgcgt cctcaaggag
       61 acgtgggcga ggtgagcgcg ccgggcacag gtagggcgga cgaggcacca accacccgtc
      121 ggctgacgat cagcggcgcc acgctggagg tcgacgacag cgcgctggcc gacccggcgg
      181 ccgccggcaa gccggccatc atcacgttgc acggcgcccc cggcatgggc agccgccgga
      241 acgattggga agtctacgcg gcgttcaccg accgcttccg cgtcgtcagt tacgaccagc
      301 gcggctcagg ggcgagttcc gacaccccgc cgtacaccca cgagcagttc gcggccgaca
      361 cggacgcggt gcgtcggacg ctcgggctcg gcgacaagat cgtcatcgcc ggcggctctt
      421 acggcggcta cttggcccag gagtacgcgc tgcgctatcc ggagcacgtg agcgccgtcg
      481 tcctgcgcga tacggccgcg cactccggac acaagggcga ggccatgcgc atcgcgctcg
      541 agagcccgta ccccatggac gagcccgagt tggagcgcct gttcggcggc atgacgacgt
      601 cggacgacga cttcagggcg ctgatcacga tgatcatgcc gctctacacg acgacctacg
      661 atccggagcg cgacgccagg caggtcgcgg ccatgcagct ccactacgcc acgcacaact
      721 acgcgttcgc gcacaaccag caggggttcg acctgcgcgg ccggctgggc gagctgcgcg
      781 tgcccgtcct gataaccgtc gggcggcacg actggatcac ccccctcgtg gccagcgagg
      841 agctgcacgc cctgatcccg gactccgagc tcgtgatctt cgagaacagc ggccacggcc
      901 cgcagaacga ggagcgcgag gcgtggcgcg cggtggtgag gcggttcctg gaccgcgtcg
      961 tgccggcgtg agcgggaacg gtcgcgtcgg tcccgtccct atcggccccg cggagtacgc
     1021 ccaacgcgcc cggacggcat ccgagcgcgc cctgggcggg gccggcgccg acgtcatcgt
     1081 cctcttcgac gaccagtaca tccagtactt cacgggcttc gtcttcagcc cgacggagcg
     1141 tccgatcgcg ctcatcgtcg ggccggatgg ggagcgcgcg ctcttcgtcc cccgcctcga
     1201 gctggagcat gcggaggcgt tggccgacgt cgaccgcgtc gtcgcctatc ccgagtaccc
     1261 gggcgagacg caccccctcg aactgctcct cgccgagacc cggcggcttg gggtcggcaa
     1321 gcgcgccatc gccgtcgacc atgacggcta cccgcccgtg atgggctacg ccccggtgag
     1381 gctcagcgac cttaccgacg cgaaggtcgt gagcctcggc gtggcgctcg acgagcagct
     1441 ggccctcaag tccgagcacg aggtcgctct ggtgcgcgag agcgcccgtt ggggcgccca
     1501 tgcccaccgt ctcctgcagg acggcaccaa gccggggcta acggaggacg ccgttgtcgg
     1561 cgccgcgtgc gcggccgcca cgcgcgccat gagcgcgagc ctcggcggac cgtaccgcca
     1621 acgcaacaga tgggtgaggg gtgccctggc catctataga ggccagatcg gaccggccgg
     1681 cgccttcccg cacgcgctcg cgcacgacgc caccttccgc gtcggcgaca cgctcgtgac
     1741 gggcgccgct gccgacgtgt acggttacct cagcgagctg gagcgcacga tgttcctcgg
     1801 cgagccgagc gccgagcaac ggcactactt cgaccacatg cttgccctgc aggacctatg
     1861 cttcgcggcc atcaggccgg gcgtcgcggc cgcctccgtc gacctcgcca tgcgcgcgta
     1921 cgtcgacgag cactccttgt ggccgcactg gcgccaccac gtcgggcacg gcctggggca
     1981 gcgcatccac gagagcccgt tcctcgacct cggctacgaa ggcgtgctcg aggccggcat
     2041 gatcctctcc gtcgagccgg gccagaaggt ctcccagggt gacgttctcg cccgctcgcc
     2101 gctggaaagc gccaagacca aggacatcac cggcggtctg ccgcgcgttg ccgaactgtt
     2161 cgaggcgcgt cgtccgaagg accacgccat catcgccgag atcgacggc
//
LOCUS       DAOQYE010000312         2209 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_56234_length_2209_cov_2.677344, whole genome shotgun sequence.
ACCESSION   DAOQYE010000312 DAOQYE010000000
VERSION     DAOQYE010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56234_length_2209_cov_2.677344"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(96..1355)
                     /locus_tag="PKN52_05915"
     CDS             complement(96..1355)
                     /locus_tag="PKN52_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNQ99516.1"
                     /translation="MQAKARQLLLAAALLLLPGAFAQTQISIGLFPDLDSHVNAILEQ
                     FQAENPDIKVEVRVLQHGDHHTALVTNLATGSGAADVVAIDVGFIARFVADGGLVDLN
                     AAPYNASQYQDLFAEYAWLQSSTTDGRLIAMPTDLGPGVMFYRRDRFEQVGASIDDVI
                     KDWDSYVEFGRQVTRDTDGDGKVDVFLIADASDVARAIMRSNIAAGDGVFFDAAGNAV
                     VESDRFKEAFRVAKEIRDAGLDARIGSWTNEWYEAFKRGTVATQLSGAWLLGHLQNWM
                     APETAGLWGASDLPNGIYGSWGGSFYGIPEQSQNKEAAWKLVQFLTTRPDVQLAAFER
                     IGAFPAVTSTYGDPLFDEEIPFLANQKARQLFARVAQNIEGRATNPGDQIAEEIVNSA
                     LSQVLDEGRSIDEALAEAQRLISRRAR"
     gene            complement(1416..>2209)
                     /locus_tag="PKN52_05920"
     CDS             complement(1416..>2209)
                     /locus_tag="PKN52_05920"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNQ99517.1"
                     /translation="ASPYFGLLIRGVEAEAEAAGMHLLVSSGHARADQERAGLEFLAD
                     RRSDALIAHIEALADEALVEYLSRPTPLILVGRKLAGHEERCVYLDNEVGGAIATEHL
                     LQHGHTRIAHITGPERFPDSRARRRGFECAMRSAGLEPDPRLVIEADFEEEGGYRAMR
                     ELLERDPGISAVFAGNDQMAAGAFAALREAGRNIPDDVSIVGYDDVLLARYLHPTLTT
                     IRQPLEEMGRAAARAALALVANERTEVRNRFEPQLVSRQSVSRLR"
BASE COUNT          304 a          702 c          838 g          365 t
ORIGIN      
        1 ccttgatcct gtggtcatgg cggccggtcc ggcgcccgcg cgcgtccggg cccccacggc
       61 gggaggtccg gacgcgcgga accggggtcg ggtctctacc tggcgcggcg gctgatgagc
      121 cgctgggctt cggcgagcgc ctcgtcgatg ctcctacctt cgtcgagcac ctgcgagagg
      181 gctgagttca cgatctcctc ggcgatctgg tcgccggggt tcgtcgcgcg gccctcgatg
      241 ttctgggcga cgcgggcgaa gagctgcctg gccttctggt tggcgaggaa ggggatctcc
      301 tcgtcgaaca gggggtcgcc gtaagtgctg gtcacggccg ggaaagcgcc gatgcgctcg
      361 aaagcggcga gctggacgtc gggcctggtg gtcaggaact gcacgagctt ccaggccgct
      421 tccttgttct gggactgctc agggatgccg tagaacgagc cgccccacga gccgtagatg
      481 ccgttcggca ggtcggaggc gccccacagg ccggccgtct ctggcgccat ccagttctgg
      541 aggtggccga ggagccaagc gccggagagc tgggtggcga cggtgccgcg cttgaacgcc
      601 tcgtaccact cgttcgtcca ggagccgatg cgggcgtcga gaccggcgtc gcggatctcc
      661 ttggcgaccc tgaaggcctc cttgaagcgg tcggactcga ctacggcgtt gccggccgcg
      721 tcgaagaaga cgccgtcgcc ggccgcgatg ttcgagcgca tgatggcgcg ggcgacgtcg
      781 gaggcgtcag cgatgaggaa gacgtcgacc ttgccgtcgc cgtcggtgtc gcgcgtcacc
      841 tggcggccga actccacgta ggagtcccag tccttgatga cgtcgtcgat gctggccccg
      901 acctgctcga agcggtcgcg gcggtagaac atgacgccgg ggccgaggtc cgtcggcatg
      961 gcgatcaggc ggccgtcggt ggtgctcgac tgcagccagg cgtactcggc gaagaggtcc
     1021 tggtactgcg aggcgttgta tggggcggcg ttcaggtcga cgaggccgcc gtcggccacg
     1081 aagcgggcga tgaagccgac gtcgatggcg accacgtcgg cggcgccgga gccggtggcc
     1141 aggttcgtca cgagggcagt gtgatggtcg ccgtgctgga ggacgcgcac ctcgaccttg
     1201 atgtcggggt tctcggcctg gaactgctcc aggatggcgt tgacgtggga gtcgaggtcg
     1261 gggaacagcc cgatcgagat ctgggtctgc gcgaacgcgc cggggaggag cagcagtgct
     1321 gcggccaaca ggagctgtct tgccttcgct tgcattctcg ccttcctttc cacgaccggc
     1381 gcggcctttg gcgggcgccg gctcttgcgc ctgggtcagc gcagtcgtga caccgactgc
     1441 cggctcacga gctgcggttc gaaacggttc ctcacctccg tccgttcgtt cgccaccagc
     1501 gcgagcgcgg cgcgggccgc ggcgcgaccc atctcctcga gtggctggcg gatggtggtg
     1561 agggtcgggt ggagatagcg tgcgagcaga acgtcgtcgt agccgacgat cgagacgtcg
     1621 tccggtatgt tgcggcccgc ctcgcgcagc gccgcgaagg cgccggccgc catctggtcg
     1681 ttaccggcga agacggccga gatgccgggg tcgcgctcga gcagctcgcg catggcgcgg
     1741 tagccgccct cttcctcgaa gtccgcttcg atgacgaggc gcgggtccgg ctcgagaccg
     1801 gcagagcgca tggcgcactc gaaaccgcgg cgcctggcgc ggctgtccgg gaagcgttcc
     1861 gggcccgtga tgtgcgcgat gcgggtgtgg ccgtgctgca ggaggtgctc ggtggctatg
     1921 gcgccaccca cctcgttgtc gaggtagacg cagcgctctt cgtgacctgc cagcttgcgc
     1981 ccgacgagga tcagcggggt cgggcggctc aagtactcga ccaacgcctc gtccgccagc
     2041 gcctcgatgt gggcgatgag ggcatcggag cgccgatcgg ccaggaactc gaggcccgcg
     2101 cgctcctggt cggcgcgggc gtggccgctc gagacgagta ggtgcattcc ggcggcctcc
     2161 gcctcggctt cgacgccgcg gatcagaagg ccgaagtagg ggctggcga
//
LOCUS       DAOQYE010000313         2208 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_56263_length_2208_cov_3.094287, whole genome shotgun sequence.
ACCESSION   DAOQYE010000313 DAOQYE010000000
VERSION     DAOQYE010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56263_length_2208_cov_3.094287"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            32..1507
                     /locus_tag="PKN52_05925"
     CDS             32..1507
                     /locus_tag="PKN52_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNQ99518.1"
                     /translation="MEDNPPDRSRRIARAPLSLLILTASGLASAQAPSTMSAAAPAVT
                     AFQVALMIVLLLASAFLSTAHTALTAVGEWKLRRLRDDGAEPSGALAILERNPARFIS
                     TLLIGNTLVNAGLAVLVTELLLRNAAALGIGLGGALVLATCVTSVLILLFGELAPKSI
                     AAHDPVRVTRLVLRPVFALSVVLYPLGLAFNWLTGQFLRVFRITPNSAALMSESELRL
                     MLQNAEESGVIEAQEQAMIKGVIDLEETVVREVMTPRVDVVGISADADLRELQAVVTE
                     HGYSRLPVYGEGVDDIKGMVYARDLLPYLGRPDALVKTKVVDLMTTVQYVPETLSVMS
                     LLREMRVRKNHLAVVIDEFGGTSGIVTLEDIIEEITGEIYDETDEDEVEDVVALEGGS
                     FRLQGGTHIETIADALKLSFDPNGDYDTVAGFLIDKLDRIPQVGEEVDFQGVKFVVDE
                     ADERRVISVIATPIVEQSPPAEAQAHEVEVETPVAVEAAEG"
     gene            complement(1512..>2208)
                     /gene="alr"
                     /locus_tag="PKN52_05930"
     CDS             complement(1512..>2208)
                     /gene="alr"
                     /locus_tag="PKN52_05930"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HNQ99519.1"
                     /translation="PAAAANVAESVARAPGLELEGLWTHFRDSDGPERTTTDRQIERF
                     VECLDEAAGKGLEIPLRHACNSAGILAYPEAHFDMVRPGILLYGYAPSDAFAGVEPRV
                     RPVMRLSAPVTFVKRVPAGTSVSYGQLWTTAEESTLATVRIGYADGYPRMLTGKAWAS
                     LNGTPCAVVGRVCMDQLLVDASTAGAVAPGDRVTLWGGDGPSADELAAQLGTVSYELI
                     TRVMPRVDKEYVG"
BASE COUNT          335 a          814 c          721 g          338 t
ORIGIN      
        1 gttcgccccc gtgctacaat ccgcccaatc gatggaagac aaccctccgg accgatcgcg
       61 gcgcatagcg cgcgccccac tatccctact catcctcaca gcctccggcc tggcgtctgc
      121 ccaggcgccg tccaccatgt cggccgccgc cccggccgtc accgccttcc aggtcgcgct
      181 gatgatcgtg ctcctgctgg cctccgcgtt cctctccacc gcccacacgg ccctcacggc
      241 cgtcggcgag tggaagctgc gacggttgcg tgacgacggc gcggagccgt ccggcgccct
      301 cgccatcctc gaacgcaacc ccgcccgctt cataagcacg ctgctcatcg gcaacacgtt
      361 ggtgaacgcc ggcttggccg tgctcgtcac cgagttgctc ttgcgcaacg ccgccgccct
      421 cggcatcggc ctcggcggcg ccctggtctt ggccacctgc gtcacgagcg tgctcatcct
      481 cctgttcggc gagctcgccc cgaagtcgat cgcggcgcac gacccggtca gggtcacgcg
      541 gctcgtgctg cggcccgtct tcgccctgtc cgtcgtcctg tacccgctcg gcctcgcctt
      601 caactggctg acgggccagt tcctgcgcgt cttccgcatc acacccaaca gcgccgcgct
      661 catgtccgag agcgagctgc gcctcatgct ccagaacgcc gaggagtccg gcgtcatcga
      721 ggcgcaggag caggccatga tcaaaggggt catcgacctc gaggagacgg tcgtgcgcga
      781 ggtcatgacc ccgcgcgtcg acgtcgtcgg catctcggcc gacgccgacc tgcgcgagtt
      841 gcaggcggtc gtcaccgagc acggctacag ccgcctcccc gtctacggcg agggcgtcga
      901 cgacatcaag ggcatggtct acgcgcgcga cctgctgccg tacctcggcc gccccgacgc
      961 cctcgtcaag accaaggtcg tcgacctcat gacgaccgtg cagtacgttc cggagaccct
     1021 ctccgtcatg agcctgctgc gcgagatgcg cgtgcgcaag aaccatctgg cggtcgtcat
     1081 cgacgagttc ggcggcacct ccggcatcgt gaccctcgag gacatcatcg aggagataac
     1141 gggggagatc tacgacgaga ccgacgagga cgaggtggag gacgtcgtcg cgctcgaggg
     1201 cggcagcttc cgcctccagg gcggcacgca catcgagacc atcgcggacg ccctcaagct
     1261 ctcgttcgac ccgaacggcg actacgacac ggtcgccggc ttcctcatcg acaagttgga
     1321 tcgcatcccc caggtcggcg aggaggtgga cttccaaggg gtcaagttcg tcgtggacga
     1381 ggcggacgag cggcgcgtga tcagcgtgat cgcgacgccg atagtggagc agagcccgcc
     1441 ggccgaggcg caggcgcatg aggtcgaagt ggaaaccccc gtcgcggtcg aggcagcgga
     1501 aggctgaggc ttcagccgac gtactcctta tcgacgcgcg gcatcacccg tgtgatgagc
     1561 tcgtaggaca ccgtgccgag ctgcgcggcc agctcatcgg cgctcggccc gtcgccgccc
     1621 cacaaggtca cgcggtcgcc gggggctacg gcgccggcgg tgctggcgtc caccagcagc
     1681 tggtccatgc acacccgccc gaccaccgcg cagggcgtgc cgttcaggct cgcccaggcc
     1741 ttgccggtca gcatgcgcgg gtagccgtcg gcgtagccga tgcgtaccgt cgcgagcgtc
     1801 gactcttcgg cggtcgtcca gagctgcccg tagctgacgc tggtgccggc cggcacgcgc
     1861 ttcacgaacg tcacgggcgc gctcaaccgc atgaccggcc tcacgcgggg ctcgaccccc
     1921 gcgaaggcgt cgctcggcgc gtagccgtac agcaggatgc cgggccgcac catgtcgaag
     1981 tgcgcttcag gataggccag gatgccggcc gagttgcagg cgtgccgcag cgggatctcg
     2041 aggcccttgc ccgccgcttc gtcgaggcac tcgacgaagc gctcgatctg ccggtcggtc
     2101 gtagtgcgct cgggaccgtc cgagtccctg aagtgcgtcc agaggccctc cagctccaag
     2161 cccggcgcgc gcgcgacgct ctccgccacg ttcgcggcgg cggcgggg
//
LOCUS       DAOQYE010000314         2206 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_56358_length_2206_cov_2.570897, whole genome shotgun sequence.
ACCESSION   DAOQYE010000314 DAOQYE010000000
VERSION     DAOQYE010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56358_length_2206_cov_2.570897"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(150..579)
                     /gene="rnpB"
                     /locus_tag="PKN52_05935"
     ncRNA           complement(150..579)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PKN52_05935"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(677..1792)
                     /locus_tag="PKN52_05940"
     CDS             complement(677..1792)
                     /locus_tag="PKN52_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HNQ99520.1"
                     /translation="MRKLLILIAALLLAGATAQDYVFGIVYDAGGKFDRSFNESTWNG
                     VQRAQQDLQADGYEVEVIEFEGTPDTAAEGQRRIALEGAELLIAPGFLQADAITSVAQ
                     EFPRTSFVIIDAVSEGDNVRSVLFKEQEGSFLVGYIAGSLTRTGVVGFVGGMDVPLIR
                     AFDLGYQEGVKAACADCTIISNYVGVTPDAWNDPARAKELASTQNAQNADIIFAAAGA
                     SGNGVIDFVNEKQCFVPSGATRSTPIDAQLDGIAKPAGYDAKCGGNAKPIFFIGVDSN
                     QNPLGDTDANAATLNFGLTSMLKRVDVAAYDSVMDVVNGEFTGGILNLGLAEAGVDYA
                     LDEYNTALIPADLVTKVNEVKQQIIDGAIVVTDYRQQ"
     gene            complement(2014..>2206)
                     /locus_tag="PKN52_05945"
     CDS             complement(2014..>2206)
                     /locus_tag="PKN52_05945"
                     /inference="COORDINATES: protein motif:HMM:NF014045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HNQ99521.1"
                     /translation="EDAYDTALIVSGDGDYVELVEAIKRRGKHVECAMFRNQSAGTLL
                     EHVDLFQNLDELDWSRIVF"
BASE COUNT          303 a          686 c          808 g          409 t
ORIGIN      
        1 ggccgaacct cggtgggccg tgtcccgcgg cagggcgcgc caccctgacg ggcctcacca
       61 gagtcggcct caccggagtt ggcctcaccg gtggcccggc gccaagggtg tctggaggta
      121 gcaatgggtg cggccccttg cggggccgca cgctggtggg ctggtaagcc gggttctgtc
      181 ggccctcccg cgagggagga cgtctggcca tctatctcgg acgcgcgtcg ccacgcgcct
      241 ctagcggtca acccggagat gttggcgagc cgggcaggct ctctctccct atcggacctt
      301 gcaccgggtg gggtttacct agctgccctt gttaccaggg gcactggtgc gctcttaccg
      361 caccgtttca cccttacgtt cgcgttcacc aagcggcgaa cgcgcccgcg gtctgctttc
      421 tgttgcactt gccgtcgcct tacgcgccca gccgttagct ggcaccctgc cctacggtgc
      481 ccggactttc ctcgatgcgc gtgcgcgcga gccggtgtga ccccgaacgg ggtcgccgtg
      541 cccgcgccgt gcgcgcctcg cggccagacc gcccacacag ccgcccgcaa ggatagcagg
      601 tcggcgcccg cgatgaagaa gcgcggaggc cctagggcct ccgcgcttcg ctaccggtcg
      661 gggctgcgcc cgggtattac tgctggcggt agtcggtgac gacgatcgcg ccgtcgatga
      721 tctgctgctt gacctcgttg accttcgtga cgaggtcggc cgggatgagg gcggtgttgt
      781 actcgtcgag ggcgtagtcg acgcccgcct cggcgaggcc gaggttgagg atgccgccgg
      841 tgaactcgcc gttcacgacg tccatgacgg agtcgtaggc ggccacgtcg acgcgcttga
      901 gcatgctcgt gaggccgaag ttgagggtgg cggcgttcgc gtcggtgtcg ccgagcgggt
      961 tctggttgct gtcgaccccg atgaagaaga tcggcttggc gttgccgccg cacttcgcgt
     1021 cgtagccggc cggcttggcg atgccgtcga gttgcgcgtc gatgggcgtg gagcgcgtgg
     1081 cgccggaagg cacgaagcac tgcttctcgt tcacgaagtc gatcacgccg ttaccggaag
     1141 cgccggcggc cgcgaagatg atgtcggcgt tctgggcgtt ctgcgtgctg gcgagctcct
     1201 tggcgcgagc ggggtcgttc caggcgtcgg gcgtgacgcc gacgtagttg ctgatgatgg
     1261 tgcagtcggc gcaggcggcc ttgacgcctt cctggtagcc gaggtcgaag gcgcggatga
     1321 ggggcacgtc catgccgccg acgaagccga cgacgccggt gcgggtgagg ctgcccgcga
     1381 tgtagccgac gaggaacgag ccttcctgct ccttgaagag gacgctgcgc acgttgtcgc
     1441 cttcggagac ggcgtcgatg atgacgaacg aggtgcgcgg gaactcctgg gcgaccgacg
     1501 tgatggcgtc ggcctgcagg aagccggggg cgatgaggag ctcggcgccc tcgagggcga
     1561 tgcggcgctg accttcggcc gcggtgtccg gggtgccctc gaactcgatg acctcgacct
     1621 cgtagccgtc ggcctggagg tcctgctggg cgcgctgcac gccgttccag gtgctctcgt
     1681 tgaaggagcg gtcgaacttg ccgcccgcgt cgtagacgat gccgaagacg tagtcctggg
     1741 cggtggcgcc ggctaggagg agtgcggcga tgaggatgag cagtttgcgc atgttgcgat
     1801 ggtcccttct gaagtgagaa gtttcaccgt gtgcggtgta gcggctggat gataccagat
     1861 gccgtcgcga gccggtcagg cgctcgtcgg ggccgccctt caccgatccc tgatggtgcg
     1921 aaggggtcct ggctggtgcg agtgggcgct tcttaggcgc ccgaagcttt cggacgccta
     1981 gaaccctcga ggcgcctgga accttttagg cgcctagaag acgatgcggc tccagtcgag
     2041 ctcgtcgagg ttctggaaga ggtcgacgtg ctcgaggagc gtgccggccg actggttcct
     2101 gaacatggcg cattcgacgt gtttgccgcg gcgcttgatg gcctcgacca gctctacgta
     2161 atcgccgtcg cctgacacga tcagcgcggt gtcgtacgcg tcctcg
//
LOCUS       DAOQYE010000315         2205 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_56398_length_2205_cov_2.856279, whole genome shotgun sequence.
ACCESSION   DAOQYE010000315 DAOQYE010000000
VERSION     DAOQYE010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56398_length_2205_cov_2.856279"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1141)
                     /locus_tag="PKN52_05950"
     CDS             complement(<1..1141)
                     /locus_tag="PKN52_05950"
                     /inference="COORDINATES: protein motif:HMM:NF009309.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter subunit D"
                     /protein_id="HNQ99522.1"
                     /translation="MTGLLEVLDRHVSIVPILLPLIAAASMLLLHERRRRAKAVVSSV
                     TLAAMFAATLRLLMVVADPSTPEIVVYRLANWSAPYGIVLVADRLSVLMVMVTVLLGA
                     AGMTYALSRWATLGPRFHSLYLLLLMGLCGAFLTGDLFNLYVFFEVLLAASYGLALHG
                     SGKRRVKAGLHYVAINLAASVVFLVGVSLVYAVTGTLNMADLAAKAGQLGPDDLVLLK
                     AGLSILGVAFLVKSGAWPLSFWLPTTYSAAAAPVAAIFAVMTKVGVYVILRLAALLWP
                     GGEALALFGSSWLLYLGLATVLFGTFGVLASSELPRMAGYLTMVSSGTLLAVIASGDP
                     VVLSSALYYLIGSTIGIGVIFLLCELVNRRDGEVAAQAVGPVFHAD"
     gene            complement(1138..1476)
                     /locus_tag="PKN52_05955"
     CDS             complement(1138..1476)
                     /locus_tag="PKN52_05955"
                     /inference="COORDINATES: protein motif:HMM:NF006573.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit C"
                     /protein_id="HNQ99523.1"
                     /translation="MEIVVAIGIGVLATTGVYLILRPRTFQVIIGLSLLTYAVNLFIL
                     AMGRLVVDGAPLLAEGADPSTFADPVPQSLVLTSIVISFSMTALFLVVLLAARGITGT
                     DHVDGDGMSA"
     gene            complement(1476..>2205)
                     /locus_tag="PKN52_05960"
     CDS             complement(1476..>2205)
                     /locus_tag="PKN52_05960"
                     /inference="COORDINATES: protein motif:HMM:NF015970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MnhB domain-containing protein"
                     /protein_id="HNQ99524.1"
                     /translation="VNVILVDFRGFDTMGEITVLAIVALTVFALLRRFRPAREVVGST
                     RQQRRQDAYDEARSDREVGDTARDYLFVPRIVMHLLFPVITLFAIHLFLRGHNEPGGG
                     FAAGVAMAIAFIIQYMARGTRWVEERLRVLPLIWIGTGLLVAALTGIGAWFFGRPFLT
                     SAHGHLHLPVIGEVSLASASLFDLGVFALVIGATVLMLIALAHQSIRSARASLSTQEL
                     EGATSAPGPAAAETAVKLGEAGGD"
BASE COUNT          417 a          672 c          830 g          286 t
ORIGIN      
        1 agtcggcgtg gaagacggga ccgacggctt gggccgccac ctcgccgtcg cggcggttca
       61 cgagctcgca gaggaggaag atgacgccga tgccgatggt ggagccgatc aggtagtaca
      121 gggcgctaga tagcacgacc gggtcgccgc tcgcgatgac ggcgagcagt gttccagacg
      181 agaccatggt gaggtagccg gccatgcgtg gcagctccga gctggccagc accccgaacg
      241 tgccgaacag gacggtggcg aggccgaggt agagcagcca cgagctgccg aacagggcga
      301 gcgcctctcc accgggccac aggagcgccg ccagtcgcag gatgacgtag acgccgacct
      361 tcgtcatgac ggcgaagatg gcggccacgg gcgccgcggc ggctgagtag gtggtcggga
      421 gccagaacga tagcggccag gcacccgact tgacgaggaa ggccacgccg aggatggaaa
      481 gcccggcctt gaggagcacg aggtcgtctg gtccgagctg accggccttc gctgccaggt
      541 ccgccatgtt aagcgtgccg gtcaccgcgt agacgaggct cacgccgacg aggaagacga
      601 ccgaggcggc caggttgatg gcgacgtagt gcaggccggc cttgacgcgc cgcttgccgg
      661 agccgtgtag ggccaggccg tatgacgcgg cgaggagcac ctcgaagaac acgtagaggt
      721 tgaagaggtc gcccgtgagg aacgccccgc acaggcccat gagcagcagc aggtaaaggc
      781 tatggaacct gggtccgagc gtcgcccagc gcgagagcgc gtaggtcatg ccggcggcgc
      841 cgaggagcac ggtcaccatg accatgagaa cggagagcct gtctgcgacg agcacgatcc
      901 cgtacggagc gctccagttc gcgagccggt agacgacgat ctcgggcgtg ctcgggtcgg
      961 cgacgaccat caggagtcgc aaggtcgcgg cgaacatggc cgcgagcgtg acagagctga
     1021 caaccgcctt ggcgcgccgc ctccgctcgt gcaggaggag catgctcgcg gcggctatca
     1081 ggggcaggag gatcgggacg atgctcacgt gccggtcgag cacctcgagc agccccgtca
     1141 tgcgctcatc ccatcgccgt ccacgtggtc ggtgccggtg atgccgcgcg cggccaggag
     1201 caccaccagg aagagggcgg tcatggagaa gctgatgacg atggacgtga gcacgagcga
     1261 ctgcggaacg gggtcggcga aagtgctagg gtcggcgcct tctgcaagga gcggcgcgcc
     1321 gtcgactacg agcctgccca tggcgaggat gaagaggttg acggcgtacg tcaggagcga
     1381 caggccgatg atcacctgga aggtacgcgg cctgaggatc aggtacacgc cggtcgtcgc
     1441 gaggacgccg atgccgatgg cgacgacgat ctccatcagt cgccccccgc ctcgccgagc
     1501 ttgacggcgg tttcggcggc ggccgggccc ggcgcgctcg tggccccctc gagttcctgc
     1561 gttgagaggc tggcgcgggc gctccggatg gattgatgcg cgagggcgat gagcatgagg
     1621 acggtcgcgc cgatgacgag ggcgaacacg ccgaggtcga agaggctggc ggacgccagg
     1681 ctcacctcgc cgatgaccgg caggtgcagg tggccgtggg cggacgtgag gaagggccgc
     1741 ccgaagaacc aggcgcctat ccctgtgagc gccgccacca gcaggccggt cccgatccag
     1801 atgagcggaa gcacgcgtag tcgttcctcg acccaacggg tgccgcgcgc catgtactgg
     1861 atgatgaacg cgatcgccat cgcgacgccg gcggcgaagc ccccgccagg ctcgttgtgc
     1921 ccgcgcagga agagatggat ggcgaagagc gtgatcacgg ggaagagcag gtgcatgacg
     1981 atgcgaggca cgaacaggta gtcgcgcgcg gtgtcgccta cttccctatc ggatcgcgcc
     2041 tcgtcgtagg cgtcctgccg tcgctgctgg cgggtgctgc caacgacctc gcgggcgggc
     2101 ctgaagcgcc tgagcaacgc gaacacggtg agggccacga tggcgaggac cgtgatctcg
     2161 cccatggtgt cgaagccgcg gaagtcgacg aggatgacgt tgacg
//
LOCUS       DAOQYE010000316         2203 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_56490_length_2203_cov_3.187616, whole genome shotgun sequence.
ACCESSION   DAOQYE010000316 DAOQYE010000000
VERSION     DAOQYE010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56490_length_2203_cov_3.187616"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(52..915)
                     /locus_tag="PKN52_05965"
     CDS             complement(52..915)
                     /locus_tag="PKN52_05965"
                     /inference="COORDINATES: protein motif:HMM:NF037183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HNQ99525.1"
                     /translation="MTYALLQAMRAIRGNWVASVSTITTMTLSLTILVGFSLVSVNLN
                     NALAQLQGELELAAYLADGANVQQLMTTVRAWPEVERLEFVSKNQALAEMVADLPALG
                     RAADLVENPLPDTFHLRLYDPSQTSIVRLRLEQLPGVDEVDDSSAAVATFLALNDVLR
                     IGGSILIVVLLGAAVFAIVNSIRAAITARKEEIDVMRLVGASRGFIRAPFLLEGLLLG
                     LISAVLSLGLAIPGYQAAVARLAEQFAFVPFVRDSALLGWIASLLAVLAILVGLVGSA
                     IAVAQHLREQD"
     gene            complement(1033..2025)
                     /locus_tag="PKN52_05970"
     CDS             complement(1033..2025)
                     /locus_tag="PKN52_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HNQ99526.1"
                     /translation="MHTICLIPGDGIGQEVVPAARRLLEATGLPIEFRLAEAGWGTFT
                     AQGTSVPEATLTAVRAADATLAGAFTSPSKRVEGHQGAIRYMRRTLDLFSNLRPARSR
                     PIPGSRDGIDMLMVRENTEGLYVEKERRYGDVAIADAVVTKRASERIARVALEQAMRR
                     RKRLAVVHKANVLPLTSGLFLDTVMSVAKEYPEVETYDIIVDAAAMKLVRDPESFDVL
                     VSTNLFGDILSDLMAGLTGGLGLAPSANLGEKYAVFEPVHGSAPDIAGRGVANPIATF
                     LTAAMLLDHLGEREAARRIERAVDEVLAEGELTPDLGGRASTESLTDAVIAALG"
BASE COUNT          331 a          716 c          826 g          330 t
ORIGIN      
        1 cgcaaagtgt agcagcggtt cgtgaccgtc ggatgacgcc ccggaggcca atcagtcctg
       61 ctcgcgcagg tgttgcgcca cggcgatggc gctgccgacg aggccgacga ggatcgcgag
      121 gacggcgagc agggaggcga tccaaccgag cagcgcgctg tcgcgtacga acggcacgaa
      181 cgcgaactgc tccgcgaggc gcgccacggc ggcctgatag ccggggatgg ccaagccgag
      241 cgagagcacg gcgctgatga ggccgagcag caacccctca aggaggaacg gcgccctgat
      301 gaagccgcgg ctcgcgccca ccaggcgcat gacgtctatc tcctccttgc gcgcggtgat
      361 ggcggccctg atggagttga cgatcgcgaa cacggccgcg ccgagcagca cgacgatgag
      421 gatggagccg ccgatgcgca agacgtcgtt caaagcgagg aacgtcgcga cggcggcgct
      481 cgagtcgtcc acctcgtcga ctcccggcag ctgctcgagg cgcagccgca cgatcgaggt
      541 ctgcgacggg tcgtacaggc gcaggtggaa ggtgtctggc agcgggttct ctacgaggtc
      601 ggccgccctg ccgagggccg gaaggtcggc gaccatctcg gccagcgcct ggttcttgga
      661 gacgaactcg aggcgctcga cctccggcca cgcgcgcacc gtcgtcatga gctgctggac
      721 gttggcgccg tccgccaggt aggccgccaa ctccagttcg ccctgcagtt gggcaagcgc
      781 gttgttgagg ttcacgctca ccaggctgaa gccgaccagg atggtcagcg agagcgtcat
      841 ggtcgttatg gtcgagacgc tggctaccca gttgccgcgg atggcgcgca tggcctggag
      901 caacgcgtac gtcatgcgcg cggctccggg cgcctgtcag cctcgtatgg cggggtcacg
      961 ggcatcaatc taccgtatgc cggatggccg ggaggaaccg cctctagacg ggcggctcct
     1021 cggcccacgg gatcatccga gcgccgcgat gacggcgtcg gtgaggctct cggtggaggc
     1081 gcggccgccg aggtccggtg tcagctcgcc ctcggcaagc acctcgtcga cggcgcgctc
     1141 gatgcgcctc gccgcctcgc gctcgccgag atggtcgagg agcatggcgg ccgtcaggaa
     1201 cgtggctatc gggttggcga cgcccctacc ggcgatgtcg ggggcgctgc cgtgcaccgg
     1261 ctcgaagacg gcgtacttct cgccgaggtt ggcgctcggc gcgagcccga ggccgcccgt
     1321 gaggccggcc atgaggtccg agaggatgtc gccgaagagg ttggtggaga cgagcacgtc
     1381 gaacgactcg gggtcgcgga cgagcttcat ggcggccgcg tcgacgatga tgtcgtacgt
     1441 ctcgacctcc gggtactcct tggcgacgct catgacggtg tcgaggaaca ggccagaggt
     1501 cagtggcagc acgttcgcct tgtgcacgac cgccaggcgc ttgcggcggc gcatggcctg
     1561 ctccagcgcc actcgcgcga tgcgctcgct ggcccgcttg gtcaccacgg cgtcggcgat
     1621 ggccacgtcg ccgtagcgcc gctccttctc gacgtacagc ccttccgtgt tctcgcgcac
     1681 catgagcatg tcgatgccgt ccctgctgcc ggggatgggg cgcgagcgcg cggggcggag
     1741 gttggagaag aggtcgagcg ttcggcgcat gtagcggatg gcgccctggt ggccctccac
     1801 gcgcttggac gggctcgtga acgcgccggc gagggtggcg tcggccgccc gcacggcggt
     1861 cagggtggct tctggcacgc tcgtgccttg ggcggtgaag gtaccccagc cggcctcggc
     1921 caggcggaac tcgatgggta ggccggtggc ctcgagcagc cggcgggcgg ccggaacgac
     1981 ctcctggccg atgccgtcgc cagggatcag gcagatggtg tgcatatgag actcctaccg
     2041 tggcgccgga cgatgttggg cagcgagtcg gcggcgcctg gcggccatgc cgcgcgtgct
     2101 ggcgccttgc tgccgcggcc ggcgaagcgg taccaagcta ccacggtccg ctccgggccc
     2161 tggccgctgg taggatggcc acgatcgagg tgccgcggtg acg
//
LOCUS       DAOQYE010000317         2202 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_56556_length_2202_cov_2.138798, whole genome shotgun sequence.
ACCESSION   DAOQYE010000317 DAOQYE010000000
VERSION     DAOQYE010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56556_length_2202_cov_2.138798"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            198..1190
                     /locus_tag="PKN52_05975"
     CDS             198..1190
                     /locus_tag="PKN52_05975"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNQ99527.1"
                     /translation="MSSDEVARLVCVGEALIDLVATERGAALSEAATFVRAAGGAPAN
                     VAVGAARLGVRTAFLGKVGADPFGDHLVSTLAAAGVDTSMVTRDAQARTGLAFVSRAP
                     DGEREFLFYRSPSADQRLRTDEVDGGRVARAEVLHFGSLGLAVSPAREATLHAASVAG
                     RAGALRSLDVNLRLDGWRSDGEARRAVLAAAAASEVVKVSEGELDFLAGGKDAAAVRT
                     LISGSTRLLVVTEGAAGAGYHLVTQDGLATGHVPGFAVDALDTTGAGDAFVAGLLTGM
                     LLDRAALVDPAALEGVLRRANACGAIATTRLGAIPALPTAGELAAFLAAVSTSA"
     gene            1322..>2202
                     /locus_tag="PKN52_05980"
     CDS             1322..>2202
                     /locus_tag="PKN52_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HNQ99528.1"
                     /translation="MTDSASVPTNVPTLADVEAARERIASGVRRTPLLPLAIERSQYP
                     VYVKPEMLQAVGSFKIRGALNFLATLPEDVRQRGVVAHSSGNHAQGVAAAAKRFGVKA
                     TIVIPEGAPAVKVENTRALGATVVRCENTQAAREGTAADIARSEGATVVPPYDHPWIV
                     AGQGTLGLEIVEDLPDVRNVLVPIGGGGLAAGVSLAVTGMVPGARVIGVEPEFAADAK
                     ESLEAGERRKWTAEQVVRTMADGVRTQSIGVLNFAILSRLLAGVVTVSEDEIARAVRH
                     YVTNAHLVVEPTGAITLA"
BASE COUNT          292 a          782 c          816 g          312 t
ORIGIN      
        1 gcgttctgac ggcacgccgg aacctgggcc cgctcacgag cccgtcggcc ccgcgggtgg
       61 gggcgtgctg ccgacctagt ccggtggcca cgccgagccg ccaccgtcat cgacctcatg
      121 ttgtatcacg tggtgcgaac gccctctgcc gcgccaggta cgtgtgggaa aacgcgcggc
      181 gcgctacatt cgacgccatg agcagcgatg aggtcgcgcg tctcgtatgc gttggcgagg
      241 ccctcataga cctggtggcc accgagcggg gcgcggcgct ctcggaggcc gcgaccttcg
      301 tgcgggcggc gggcggggcg cccgcgaacg tcgccgtcgg cgcggcgcgg ctaggcgtca
      361 ggacggcgtt cctcggcaag gtaggcgccg acccgttcgg cgaccacctg gttagcacct
      421 tggccgcggc cggtgtcgac acgagcatgg tgactcgcga cgcgcaggcg cgcacaggcc
      481 tcgcgttcgt cagccgcgca cccgacggcg agcgggagtt cctcttctac cgcagcccct
      541 ccgccgacca acgcttgcgt acggacgagg tcgacggtgg gcgcgtggcg cgggccgagg
      601 tcctgcactt cgggtctctc ggactggcgg tctccccggc gcgggaggcc accctccacg
      661 ccgcctccgt ggccggacgt gccggggcgc tgcgctcgct cgacgtgaac ctccggctcg
      721 acgggtggcg gtccgacggc gaagcgaggc gcgcggtcct ggcggccgcg gccgcgagcg
      781 aggtggtgaa ggtgagcgag ggggagctcg acttcctggc cggcggcaag gacgccgcgg
      841 cggtgcggac gctcatctcc ggctctacca gactcctcgt cgtgaccgag ggcgcagcgg
      901 gcgccggtta ccacctcgtt acccaggacg ggttagcgac ggggcatgtg ccgggcttcg
      961 ccgtcgacgc gctggatacg actggcgcgg gtgacgcgtt cgtggcgggg ctcctcacgg
     1021 gcatgctgct ggatcgggcc gccctggtag atccggctgc gctagagggc gtgctgcggc
     1081 gcgccaacgc ctgcggcgcc atcgccacca cgcgcctcgg cgccatcccg gcgctgccca
     1141 cggcgggcga gctcgcggcg ttcctcgcgg ctgtgtcgac tagtgcctga gagcggacca
     1201 gcggctgcct tcgcgaccgc gttggcgctc cggcgcgacg gcagccgcta ggaccgcctg
     1261 gacgacccgc ctccgtcgcg gttgcccaac gctccgcgcc gtcgcgtgcg acactgagcg
     1321 cgtgaccgac tccgcgtccg ttcccaccaa cgttccgacc ttggcagacg tcgaggcggc
     1381 gcgcgagcgc atcgcttcgg gcgtgcggcg cacaccgctc ctgccgcttg ccatcgagcg
     1441 gtcccagtac cccgtctacg tcaagcccga gatgctgcag gcggtgggct ccttcaagat
     1501 ccgcggcgcg ttgaacttcc tggccacgct gcccgaggac gttaggcagc ggggcgtggt
     1561 ggctcattca agcggcaacc acgcgcaggg cgtcgcggcg gccgccaaac gcttcggcgt
     1621 gaaagcgacg atcgtgatcc ccgagggcgc gccggccgtg aaggtggaga acacccgcgc
     1681 cctcggcgcg accgtcgtgc gttgtgagaa cacgcaggcc gcccgcgagg gtaccgcggc
     1741 ggacatcgcc aggagcgagg gcgccactgt cgtgccgccg tacgaccacc cttggatcgt
     1801 ggccgggcaa ggcaccctgg ggctcgagat cgtcgaagac ctgccggacg tgaggaacgt
     1861 gctcgtgccc atcggcggcg gcggcctcgc cgccggcgtg agcctcgcgg tcacaggcat
     1921 ggtccctggc gcgcgcgtca tcggtgtgga gccggagttc gcggccgacg ccaaggagtc
     1981 gctggaggcc ggcgagcggc gcaagtggac cgccgagcag gtcgtaagga cgatggccga
     2041 cggcgtgcgc acccagagca tcggcgtgct gaacttcgcc atcctctccc gactgctggc
     2101 gggcgtcgtg acggtctcgg aagacgagat cgcccgcgcc gtcaggcatt acgtcaccaa
     2161 cgcgcacctg gtcgtcgagc cgacgggggc catcaccctc gc
//
LOCUS       DAOQYE010000318         2200 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_56619_length_2200_cov_2.916084, whole genome shotgun sequence.
ACCESSION   DAOQYE010000318 DAOQYE010000000
VERSION     DAOQYE010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56619_length_2200_cov_2.916084"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2200
                     /locus_tag="PKN52_05985"
     CDS             <1..>2200
                     /locus_tag="PKN52_05985"
                     /inference="COORDINATES: protein motif:HMM:NF016944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HNQ99529.1"
                     /translation="VARGGIRWSDRPDDFRTEVLGLLKTQTTKNAVIVPVGSKGGFVL
                     KGAPSDRDQLRDFVRTQYRTYIRALLDLTDNLVDGEIVHPKGLVIYDEPDPYLVVAAD
                     KGTATFSDLANATAAEYGFWLGDAFASGGSAGYDHKGMGITARGAWEAVKRHFAELGL
                     DVFKDTFTVAGIGDMSGDVFGNGLLYTDRIRLQAAFNHMHVFLDPDPDPKASFAERKR
                     LFELPRSTWADYDASTISRGGGVFERSAKSIPLSPEVKRMLGVDDDALSGQDLIRAVL
                     RMEVDLLWNGGIGTYVKASSERNAEAGDSANDGVRIDGAELRATVVGEGGNLGFTQLG
                     RIEYALAGGRLNTDAIDNSAGVDTSDHEVNIKILFQPLVAGAAVTLEGRNELLKSMSD
                     DVAQLVLSHNRAQALALSLAQRASREDLGLYSSLLDYLVENSGLNPHVEKLPTARQLE
                     ERRRAGQGLTRPELSVMLAYVKMGLYRRLLETDLPSEPRLQHYLVEYFPPALRERFPE
                     AMANHRLHREIVATVLTNTLVDELGLAFVHKAMRQNGVTPIEVVRATLLALELLDAKA
                     VRAHLDARAADVPAEAYYTALEQFVAAVESVVGWMLFNDVGHGGFEEAVTAYQPPLGE
                     LRDALAEQLPEVERDRRAAAVADLVEQGFDEADAEMITGFDYLADSLRIVDIASETGV
                     SLGYAAQRFYQLGERLSLGWLRETIEALPSSGQWEQVALVGLVMDLRSVQQ"
BASE COUNT          356 a          800 c          715 g          329 t
ORIGIN      
        1 ggtagcccgc ggcggcatcc ggtggtcgga caggccggac gacttccgta ccgaggtcct
       61 cggcctgctg aagacccaga cgacgaagaa cgccgtgatc gtgcccgtcg gctcgaaggg
      121 cggcttcgtc ctcaagggcg cgccctccga ccgcgaccag ctccgcgact tcgtgcgcac
      181 gcagtaccgg acctacatcc gggccctgct ggacctcacc gacaacctgg tcgacggcga
      241 gatcgtgcat ccgaaggggc tcgtcatcta cgacgagccc gacccgtacc tcgtcgtggc
      301 ggccgacaag ggcaccgcta cgttctccga cctggccaac gccacggccg ccgagtacgg
      361 cttctggctt ggcgacgctt tcgcctccgg cggttccgcc ggctacgacc acaagggcat
      421 gggcatcacg gcccgcgggg cctgggaggc cgtcaagcgt cacttcgccg agctcgggct
      481 agacgtgttt aaggacacgt tcaccgtcgc cgggatcggc gacatgtccg gcgacgtgtt
      541 cggcaacggg ctgctctaca ccgaccgcat ccggctgcag gcggcgttca accacatgca
      601 cgtcttcctc gaccccgacc cggatcccaa ggcctcgttc gccgagcgca agcggctctt
      661 cgaacttccg cgctctacgt gggccgatta cgacgcgtct acgatcagcc gcggcggcgg
      721 cgtgttcgag cgctccgcca agtccatccc gctgagcccc gaggtcaagc gcatgctggg
      781 ggtcgatgac gacgccctga gcggccagga cctcatcagg gccgtgctgc gcatggaggt
      841 cgacctcctc tggaacggcg gcatcgggac gtacgtcaag gcgtcgagcg agcgcaacgc
      901 cgaagccggc gactcggcga acgacggcgt gcgcatcgac ggggccgagc tgcgcgccac
      961 ggtcgtcggc gaaggcggga acctgggctt cacgcaactc ggtcgcatag agtacgcgct
     1021 cgccggcggc aggctgaaca ccgacgcgat cgacaacagc gccggcgtcg acacgtccga
     1081 ccacgaggtc aacatcaaga tcctcttcca gccgctggtg gccggcgccg ccgtgacgct
     1141 ggaagggcgc aacgagctcc tgaagtccat gtccgacgac gtggcgcagc tcgtcctgtc
     1201 gcacaaccgc gcgcaggccc tcgccctcag cctcgcccag cgggccagca gggaggacct
     1261 cgggctttac tcgtcgctcc tcgactacct cgtcgagaac tccggcctca acccgcacgt
     1321 cgagaagctc ccgacggcca ggcaactgga agagcgccgc agggccggcc aggggctgac
     1381 gcgccccgag ctctccgtga tgctcgccta cgtgaagatg ggcctttacc gccgactgct
     1441 cgagaccgac ctgcccagcg agcctcggct gcagcactac ctcgtcgagt acttcccgcc
     1501 cgcgttgcgc gagcgcttcc ccgaggctat ggcgaaccac aggctgcacc gcgagatcgt
     1561 cgctaccgtc ctgaccaaca ccctggtgga cgagctcggg ctcgcgttcg tgcataaggc
     1621 tatgcggcag aacggcgtca cgcccatcga ggtcgtgcgc gccaccttgc tggcgctcga
     1681 actcctcgac gccaaggcgg tccgcgccca tctggacgcc agggcggccg acgtgcctgc
     1741 cgaggcctac tacaccgcgc tcgagcagtt cgtcgccgcc gtcgagagcg tcgtcgggtg
     1801 gatgctcttc aacgacgtcg gccacggcgg cttcgaggag gccgtcaccg cttaccagcc
     1861 gccgctcggt gaactccgag acgccctcgc tgagcagcta cccgaggtgg agcgcgaccg
     1921 ccgcgcggcg gccgttgccg acctcgtcga gcaaggcttc gatgaggccg acgccgagat
     1981 gatcaccggc ttcgactacc tggccgacag cctgcgcatc gtcgacatcg cgtccgagac
     2041 gggcgtctcg ctcggatacg ccgcgcaacg cttctaccag ctcggcgagc ggctctcgct
     2101 cggctggctg cgcgagacca tcgaggcgtt gccgagctcc ggtcagtggg agcaggtggc
     2161 gctcgtcggc ctcgtcatgg acctccggtc cgtccaacag
//
LOCUS       DAOQYE010000319         2199 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_56674_length_2199_cov_2.316231, whole genome shotgun sequence.
ACCESSION   DAOQYE010000319 DAOQYE010000000
VERSION     DAOQYE010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56674_length_2199_cov_2.316231"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..809
                     /locus_tag="PKN52_05990"
     CDS             <1..809
                     /locus_tag="PKN52_05990"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNQ99530.1"
                     /translation="LDGANLRHVVVPFAGVAQELRARALERPHLSVHNSHYNAAMVAH
                     HALALAMACAYRTVAADRALRRGDWGDDTAAEHSSTFLPGKVALLVGFGHIGKALVAP
                     LSALGMDVRAWRRRPTDDGVPTYGPAELSQALAEADLAVVSLPATPETEGLIGPEELG
                     TMKRSAILVNVGRGSVIHEEALYRALAEHRLRAAGIDVWYRYPEHRGKLARVFPSDFP
                     FQELDNVVMSPHRGNDIMEWREASARDALLTLAALARGESRNPVDLTSGY"
     gene            complement(792..1511)
                     /locus_tag="PKN52_05995"
     CDS             complement(792..1511)
                     /locus_tag="PKN52_05995"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNQ99531.1"
                     /translation="MTPSDAVRDAWSREMSQTGPAPRPSAHLGTPVAEPSARARKKAD
                     RRLTILESALALIEERGLKGATFELIAERSGVSRGTVFNYFPNKETILVAYFARQLDD
                     LARRAAELRARPTGPGAPEFDGLAEIRYLFAELAGFVQAHRELVLPISFELLDPNEER
                     SRAAYLAIPLVELLRSALKRAQAAGTVRADYSAERLARTLANVYFITALQWAAYRHDR
                     DAREEFAVALRLALEGLVAAG"
     gene            complement(1621..1971)
                     /gene="rplT"
                     /locus_tag="PKN52_06000"
     CDS             complement(1621..1971)
                     /gene="rplT"
                     /locus_tag="PKN52_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HNQ99532.1"
                     /translation="MPRAKTGIVRRRRHQKVLKRAKGYWGLRSKSFRVAQQTLLNAAD
                     YSYRDRRDKKTQFRRLWIARINAAARQEGMSYSRFVAGLRKAGVQLDRKVMADLAVRE
                     PAAFKALVEVAAKA"
     gene            complement(1983..>2199)
                     /gene="rpmI"
                     /locus_tag="PKN52_06005"
     CDS             complement(1983..>2199)
                     /gene="rpmI"
                     /locus_tag="PKN52_06005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HNQ99533.1"
                     /translation="VQERDSMPKLKTHKGTKARVKITGRGKVKAMRSGKRHLNYKKSG
                     RKIRQGRNDLVVAKPEAERIKALLPYD"
BASE COUNT          327 a          741 c          795 g          336 t
ORIGIN      
        1 tgcttgacgg cgccaacttg cggcacgtgg tggtgccctt cgccggggtg gcgcaggagt
       61 tgcgcgcccg ggcgctcgag cggccacacc tgagcgtgca caactcgcac tacaacgccg
      121 ccatggtcgc ccatcacgcg ctggccctcg ccatggcgtg cgcctaccgc acggtggccg
      181 ccgaccgggc gttgcgccgg ggcgattggg gcgacgacac cgccgccgag cactccagca
      241 cgttcctgcc cggcaaggtg gcgctgctcg tcggcttcgg gcacatcggc aaggcgctcg
      301 tggcgccgct gagcgcgctc ggcatggacg tgcgggcgtg gcggcgccgg ccgaccgacg
      361 acggcgtacc tacctacgga cccgccgagc tcagccaggc gctcgccgag gccgacctgg
      421 cggtggtaag cctgcccgcg acgccggaga cagagggact gatcggcccg gaggaactcg
      481 ggacgatgaa gcgcagcgcc atcctggtca atgtcggccg cggcagcgtc atccatgagg
      541 aggccctgta ccgggccctg gccgagcacc ggctgagagc cgccggcatc gacgtctggt
      601 accgctaccc ggagcaccgc ggcaagctgg cgcgcgtgtt cccgagcgac ttccccttcc
      661 aggagctgga caacgtcgtc atgagccccc accgcggcaa cgacatcatg gagtggcgcg
      721 aggcgtcggc ccgcgatgcg ctacttacgc tcgccgcgct ggcgcggggc gagagcagga
      781 acccggtcga cctaaccagc ggctactagc ccctcaagcg cgagccgcaa cgccaccgcg
      841 aactcctcgc gcgcgtcgcg gtcgtggcgg taggcggccc actgcagggc ggtgatgaaa
      901 tagacgttgg cgagggtgcg cgccaagcgt tcagccgagt agtccgcacg cacggtgcct
      961 gctgcctggg cgcgcttcaa cgccgaacgg agcagctcca ccagcgggat ggccaggtag
     1021 gcggcgcgcg agcgctcctc gttggggtcg aggagctcga aggagatggg gaggacgagt
     1081 tcgcggtgcg cctggacgaa cccggccagc tcggcgaaga ggtagcggat ctcggccagg
     1141 ccgtcgaact ccggcgcgcc tggccccgtc ggccgcgcgc gcaactcggc ggcgcgccgg
     1201 gcgagatcgt cgagctgcct cgcgaagtag gcgacgagga tggtctcctt gttggggaag
     1261 tagttgaaga cggtcccgcg cgagacgccg gagcgctcgg cgatgagctc gaaggtggcg
     1321 cccttgagac cccgttcctc gatgagggcg agggcgctct ccaggatcgt caggcggcgg
     1381 tccgccttct tgcgcgcgcg ggcgctcggc tcggcaacgg gcgtcccgag gtgcgccgac
     1441 ggacgaggag caggcccggt ctggctcatt tcgcgagacc aggcgtcccg gacggcgtcg
     1501 gaaggggtca ccgaggcatc ataccggcag ggacaagaag caccgggcgc cgccaggacc
     1561 gtgtcctgcg gcgcccggtt agcccaaggc tgatcgacgc gaggcgcgtc agcggaacgc
     1621 tcaggccttg gcggccacct cgacgagcgc cttgaaggcg gccggctcgc gcacggccag
     1681 gtccgccatg accttgcggt cgagctggac gcccgcctta cgcaggccag cgacgaagcg
     1741 cgagtagctc atgccttcct gccgcgcggc ggcgttgatg cgcgcgatcc agagtcggcg
     1801 gaactgggtc ttcttgtcgc ggcggtcgcg gtaggagtaa tcggccgcgt tgaggagcgt
     1861 ctgctgcgcg acgcggaagc tcttggagcg caagccccag tagcccttgg cgcgcttgag
     1921 caccttctga tgacggcgac ggcgaacgat gccggtcttg gcgcgtggca ttcttcaacc
     1981 cctcaatcgt agggcagcag cgccttgatg cgctctgcct ccggcttggc tacgacgagg
     2041 tcgttgcgtc cctggcggat cttgcggccc gacttcttgt agttcaggtg ccgcttgccg
     2101 gagcgcatcg ccttgacctt acctcgccct gtgatcttga cacgggcttt ggtgccctta
     2161 tgggtcttga gtttcggcat gctatctctc tcctgtact
//
LOCUS       DAOQYE010000320         2199 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_56677_length_2199_cov_2.120336, whole genome shotgun sequence.
ACCESSION   DAOQYE010000320 DAOQYE010000000
VERSION     DAOQYE010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56677_length_2199_cov_2.120336"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..650)
                     /locus_tag="PKN52_06010"
     CDS             complement(<1..650)
                     /locus_tag="PKN52_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99534.1"
                     /translation="MKPLRVAFFGSPEFALPTFEALSRQHDLVIVVSQPDRPAGRGLG
                     SRRPAIAAAQASAAHQRGGRHRQLQQIENERVEVGQRAGDAIGRMFHDAGAKAEVGAV
                     ARNGQQLDVTRAGGLSKVCEHPPPVTHQGFGEQIALGFGKRQGEQRAVVARLERDHNK
                     VLGAAASSSSAASAAGSVTGAWQVKLRQTYAAGLKPRPAAVSRPEAIAPIQGNVAA"
     gene            complement(652..1287)
                     /gene="def"
                     /locus_tag="PKN52_06015"
     CDS             complement(652..1287)
                     /gene="def"
                     /locus_tag="PKN52_06015"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNQ99535.1"
                     /translation="MIHPIRLFGDPMLRRRADPVTDFGPELAKLAADMLETMYAAEGV
                     GLAGPQIGVPRRIFVALEVPQAADDDDDAPWENRREHVMVNPVITRQTGVQHGRDGCL
                     SIPGIAVEDVPRGLVVHVDYQDVAGARHSLVAEGYFAHVLQHENDHLNGVLFFDHLPP
                     DRRGAFLEENRKELADMQRQAKAFLRDLRAGKPASGHAAAPSGARPRAEHH"
     gene            complement(1328..>2199)
                     /locus_tag="PKN52_06020"
     CDS             complement(1328..>2199)
                     /locus_tag="PKN52_06020"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HNQ99536.1"
                     /translation="PSKLAALLVAFFFWTVVTSSSTSTTQRSLLVDLEVDGVEATTVA
                     VGVPATVEVSVSGPSGRVDRLRADQLRATLDLSDAASDFERRIQVQTPPDIRLLQVTP
                     SDVIGFLEQVVQRVMPVEVALLGDPPPAAELDSSAAPATVTIQGRSQVLDQVARVVAL
                     SPARGGTVELLALDARGLPVSEVTFTPPSVAVTFAISDLLRSKVVPLSFTPPPTMTSQ
                     SIILSSDTVAIVGSPEALDAITSVAATVDPPTGERAPGRYTFEVHLTLPSGVVALATP
                     TATVVYGDNPVPQ"
BASE COUNT          314 a          729 c          811 g          345 t
ORIGIN      
        1 ctggccgcca cgttgccctg gatcggcgcg atggcttctg gacgggagac agccgcaggg
       61 cggggcttca gccccgccgc gtacgtctgc cgcaacttca cctgccaggc cccggtgacc
      121 gaacccgcgg cgctggcggc gctgctggac gaagccgccg ctcccaggac cttgttgtga
      181 tcacgctcga gtcgcgccac gacggcgcgc tgctcgccct gacgtttgcc gaagccaagg
      241 gcaatctgct caccgaagcc ttggtgcgtg actggaggcg ggtgctcgca gaccttcgag
      301 agcccacccg cgcgcgtcac gtcaagctgc tgaccattac gggcgacggc gccgacttca
      361 gctttggcgc cagcatcgtg gaacatgcgc ccgatcgcat cgccggcgcg ttgcccgact
      421 tcaacgcgtt cattctcgat ctgctgcagt tgccgatgcc gaccgccgcg ctggtgcgcg
      481 gccgatgcct gggcggcggc gatggccggt cgcctcgaac caagcccgcg tcccgccggt
      541 cgatcgggtt gcgacactac gatgaccagg tcgtgctgac gggagagggc ctcgaacgtc
      601 ggcagcgcga actctgggct gccgaagaag gcgacgcgga gtggcttcac gtcagtggtg
      661 ctcggcccgc ggcctggcgc cggacggcgc agcagcatgc ccgctcgcag gcttgcctgc
      721 ccggaggtcg cgcaggaacg ccttggcctg ccgctgcatg tccgcgagct ccttgcggtt
      781 ctcctcgagg aaggcgccgc gcctgtcggg gggtaggtgg tcgaagaaca ggacgccgtt
      841 caggtggtcg ttctcgtgtt gcagtacgtg ggcgaagtag ccctccgcca ccagcgagtg
      901 ccgtgcccct gcgacgtctt ggtagtcgac gtgtacgacg agaccgcggg ggacgtcctc
      961 gacggcgatg ccggggatac tgaggcagcc gtcgcgtccg tgctgcaccc ccgtctggcg
     1021 cgtgatcacg gggttgacca tgacgtgttc ccgccggttc tcccacggcg cgtcatcgtc
     1081 atcgtcggcc gcctgcggta cttccagggc gacgaagatg cgtctcggca cgccgatctg
     1141 cggtcccgca agaccgacgc cctcggccgc gtacatggtc tccagcatgt cggcggccag
     1201 cttggcgagc tcggggccga agtcggtgac ggggtcggcg cggcgcctga gcatcgggtc
     1261 gccgaacagc ctgatggggt ggatcaacgt tcctcctgct tggccgcgct cgagacctga
     1321 ccgggcctta ctgcggcacc gggttgtcgc cgtacaccac ggtggccgtc ggcgtggcta
     1381 gggccaccac cccgctcgga agcgtcaggt ggacttcgaa agtataccgg ccgggcgcgc
     1441 gctcgccggt aggcgggtct accgtggcgg cgacgctggt gatggcgtcg agagcctcgg
     1501 gggagccgac tatggcgacc gtgtcggagc tcaggatgat gctctgtgag gtcatggtcg
     1561 gcggaggcgt gaagctcaac ggcaccacct tgctgcggag caggtcggag atggcgaacg
     1621 tgacggcgac gctcggtggg gtgaaggtca cctccgagac ggggaggccg cgagcgtcga
     1681 gcgcgagcag ttcgacggtg ccgccccggg ccggagagag cgccaccacc ctggcgacct
     1741 gatcgaggac ctggctgcgc ccctggatcg tcacggtggc cggtgcggcg ctggagtcga
     1801 gctccgcggc gggcgggggg tcgccgagca gcgcgacctc cacgggcatg acgcgctgca
     1861 cgacctgctc gaggaagccg atgacgtcag acggggtcac ctgtagtagc cggatatcgg
     1921 ggggcgtctg tacctggatg cggcgctcga agtcgctggc ggcgtcgctc aggtcgaggg
     1981 tggcgcgcag ctggtcggcg cgcagccggt cgacgcgtcc gctcgggccg gagacgctca
     2041 cctcgaccgt cgcgggcacg ccgacggcga ccgtggtcgc ttcgacgccg tcgacctcga
     2101 ggtcgaccag cagcgaccgc tgcgtcgtgc tcgtgctcga ggaggtcacc accgtccaga
     2161 agaagaaggc gacgaggagc gcggcaagct tgctcggcc
//
LOCUS       DAOQYE010000321         2195 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_56836_length_2195_cov_2.540187, whole genome shotgun sequence.
ACCESSION   DAOQYE010000321 DAOQYE010000000
VERSION     DAOQYE010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56836_length_2195_cov_2.540187"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(69..866)
                     /locus_tag="PKN52_06025"
     CDS             complement(69..866)
                     /locus_tag="PKN52_06025"
                     /inference="COORDINATES: protein motif:HMM:NF014600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HNQ99537.1"
                     /translation="MRRGVRIVAVLALLLVVSWILLDPASVLRDWLSGTVTGAAEPSP
                     EPLSTLPTPPTCTFGDEPAPGREQADWKVTLVDTLFALPPEYVPDDLVDLSERLADVS
                     RFAAAPGLRLRAEAAQQLREMFRDAEASRIYLEVVSAYRSFDYQRETFARWVATDGYD
                     LAIRTSARAGHSEHQLGSAVDLRIRGGAEPWDLDDWGETPEGRWVRDNAWRFGFVLSY
                     PPGMEQVTCYAYEPWHYRYVGAGMAEAIRSSGLTLRVYLSTFLYADP"
     gene            complement(962..1345)
                     /locus_tag="PKN52_06030"
     CDS             complement(962..1345)
                     /locus_tag="PKN52_06030"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HNQ99538.1"
                     /translation="MTPLSMELLRVLAEATAPLTAREILSELRLSYPHVGFDSVYRNL
                     FVLANCGTVNQLNLQNRDAARFELQDEHHHHAICLACGTVVRVDACPTTGPGTALPEI
                     PGFRPTNHVFEIYGYCGACQQGPSS"
BASE COUNT          426 a          689 c          690 g          390 t
ORIGIN      
        1 ggctcggtag gtgtctgcgg gcaagccgta ggaccccgcg gcccgtggcg acgccagtgc
       61 cgtctcgctc acgggtcggc atacaggaac gtcgatagat acacgcgaag cgtaagtcca
      121 gacgagcgta tggcctcagc catcccggcg cctacgtatc ggtagtgcca cggttcgtac
      181 gcatagcacg tcacttgctc catgccaggc gggtagctga ggacgaaacc gaagcgccag
      241 gcgttgtcac gaacccatct accttccggg gtctcgcccc agtcgtcgag gtcccacggc
      301 tcggcgccgc cccgaatgcg caagtcgact gcgctgccca actggtgttc ggaatggccg
      361 gcgcgcgcgc tcgtccttat ggcgagatcg tagccgtcgg tcgccaccca gcgcgcgaaa
      421 gtctcgcgtt ggtagtcgaa ggagcggtag gccgagacga cctccaagta gatcctcgag
      481 gcttctgcgt ctctgaacat ctctcggagc tgctgagcgg cttcagctct caaccgcaga
      541 cccggggcgg ccgcgaagcg agagacatcg gctaggcgct cgctcagatc aaccaggtcg
      601 tcgggcacat actctggcgg gagcgcgaac agcgtgtcga ccaacgtgac cttccagtcg
      661 gcctgctccc gtcctggagc gggctcgtcg ccgaaggtgc aggttggtgg cgtgggcagg
      721 gtcgacaacg gttctggcga cggttcggca gccccggtca cggtgccgga gagccaatcg
      781 cgcagcaccg acgcgggatc aaggaggatc caggacacga ctagcagtag cgcgagcaca
      841 gccacgatgc ggacaccacg cctcatggaa cctccgccat gctgttcggt agagcctggg
      901 gtgtgagcga agccgggggg agtgatggca tgaggtacct cgatcgggaa gcgcgatgac
      961 cctagctaga aggaccctgc tgacaagccc cgcagtagcc gtagatctcg aacacatggt
     1021 tggtcggcct gaaacccggg atctccggca gagcggtccc cggccccgtg gtcgggcatg
     1081 catcgacgcg aacgaccgtt ccgcacgcta gacagatggc gtgatggtga tgttcgtcct
     1141 ggagctcgaa gcgcgccgcg tcgcggttct ggaggttcag ctggttgacc gtcccgcaat
     1201 tggcgaggac gaagaggtta cggtagacgg agtcgaaacc aacatgcggg taggagagcc
     1261 tcagctcgga aaggatctca cgcgccgtta acggtgccgt cgcctcggcc agaacgcgca
     1321 gcagttccat acttagcggc gtcaaccggt aaccactgga gcgaagcgtc tcggctgcgg
     1381 acccgatgat cttctctttt ggtcttttca taccgatact cccaccgcaa ctcgaacagg
     1441 ggctgcacaa cgccgtcgcg acgaccaagg aggcgaaccc atctatgtgc cgaagagctg
     1501 gcgcggttct cctcggcccg agtgtcttgt ctccctcacg tgttcatggt cgctggacta
     1561 atcgttaata ggaaaattcc tatctggggc gtactgtacg atggccgcta tccggcgtca
     1621 agcagtagcc ggttcatgct taccgaccta ccctccgccc cgtcggcggc agcatgtctc
     1681 ctcgagagag actcgtacag cctattgacg ccctttgcgg gcagcattta gagtttcaaa
     1741 tcggcatcgg cacctccacc gcctacaccc gcgcggcggt tcgccccgtt tgaaagccga
     1801 tccactggga ggtgactgat ggacgatgac gacagtaaaa cttcgacgcc cacagcccta
     1861 acagtatcga cccggccttg tcgggagcct cgctgcgaag cgagtgctag gttctaatct
     1921 cgctggcgtc tccgcgccga cggctccgga gtaccggcta gacaccgatc ttgcacagag
     1981 tttcgcgccc ggctctcact cgcagctaag aggagcgaac cggatgcgag tgcagaacat
     2041 acgttggttc atacgcgccc actcaacatt tgcgccaaga ggccctcgat gagcgaggga
     2101 ggcccatatc aaggctacgt acgaaacgct ctggcgcatg accgtatcgg ccgcgaggtc
     2161 gcgacccgcg cccgtgcgtt cgacatgacc gtggt
//
LOCUS       DAOQYE010000322         2191 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_57005_length_2191_cov_2.910112, whole genome shotgun sequence.
ACCESSION   DAOQYE010000322 DAOQYE010000000
VERSION     DAOQYE010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57005_length_2191_cov_2.910112"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2191)
                     /locus_tag="PKN52_06035"
     CDS             complement(<1..>2191)
                     /locus_tag="PKN52_06035"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HNQ99539.1"
                     /translation="TRNRLALPRDQQLRILTKLTDATIFEEFLQKKYVGAKSFSLEGS
                     ETLIPLLDLAIEKAGAQGVEEVVLGMAHRGRLNVLANILGKNTRTIFREFDDKDPELY
                     LGRGDVKYHLGYSNDWVTSQGKRLHLSLAFNPSHLEFVNTVVLGRARAKQDRLGDARR
                     ERVLPILIHGDASFIGQGIVQETLNLSGLVGYRVGGALHIIVNNQVGFTTGPRQGRST
                     TYASDIAKMLQSPVFHVNGEDPEAVAQVIELAMDFRSEFKRDVVVDMYAYRRFGHNET
                     DEPSFTQPVMYSKIHSRKGVREAYLGYLTRLGEVTQADAERIADERRAHLEQELSVAR
                     SGDFKLQYSTLQGVWDKYQGGADASLPDPDTGIALADAKAWLSALTVVPEDFNLNQKI
                     ARILKNRAAQAAGEMPLDWAAAEALAFASFVAGGTKVRFTGQDVERGTFSHRHAVFHD
                     AQDGHEYVPMSSIEARPGLFEIHNSPLSETGVLGFEYGYSLDAPSGLTLWEAQFGDFV
                     NVAQTIIDQFIASAEEKWSRLSGLVMLLPHGFEGQGPEHSSARLERFLQLCADDNLQV
                     AYPTTPAQIFHLLRRQVVRPYRKPLIVMSPKSLLRHPQAVSTLQELTTGKFRRVIPDE
                     TVEAEGVKRILLTSGKFYYDIIAERDRRGAKDVAVVRLEQLYPLRDSELEAALAPYPA
                     AAKVVWVQEEPENNGAWHFMLVRFGQSMFGRPFSHASRPASASPATGS"
BASE COUNT          332 a          692 c          779 g          388 t
ORIGIN      
        1 cggagccggt ggccgggctc gccgaggccg gcctggaggc atggctgaac gggcgtccga
       61 acatgctctg gccgaagcgg acgagcatga aatgccatgc gccgttgttc tccggctcct
      121 cttgcaccca gacgaccttg gcggcggccg gatagggggc gagggcggcc tccagctccg
      181 aatccctgag ggggtagagc tgctcgagcc gcacgacggc cacgtccttg gccccgcgcc
      241 ggtcgcgctc ggcgatgatg tcgtagtaga actttccgct cgtgagcagg atgcgcttca
      301 cgccctccgc ctcgacggtc tcgtccggga tgacccgccg gaacttaccg gtggtcaact
      361 cctgcaaggt gctcacggcc tgggggtgcc gcaggaggct cttcggcgac atcacgatga
      421 ggggcttgcg gtaagggcgc accacctggc gcctgaggag gtggaagatc tgggcgggcg
      481 tagtggggta cgcgacctgc aggttgtcgt cagcgcacag ctgcaagaac cgctccagac
      541 gagcgctcga gtgctcgggg ccctggccct cgaagccgtg cggcaggagc atgacgaggc
      601 cggagagccg cgaccacttc tcctccgcgc tggcgatgaa ctggtcgatg atcgtctgag
      661 cgacgttgac gaagtcgccg aactgcgctt cccagagcgt caaacccgac ggggcgtcga
      721 ggctatagcc gtactcgaag ccgagcacgc ccgtctcgga gaggggcgag ttgtggatct
      781 cgaagaggcc cggcctagcc tcgatgctgc tcatgggcac gtactcgtgg ccgtcctgcg
      841 cgtcgtggaa gacggcatgc ctatggctga aggtgccgcg ctcgacgtcc tggccggtga
      901 agcgcacctt cgtgccgccg gccacgaagc tcgcgaaggc gagcgcctcg gcggccgccc
      961 aatcgagcgg catctcgccg gcggcctgag ccgcgcggtt cttgaggatg cgagcgatct
     1021 tctggttgag gttgaagtcc tccggcacga cggtgagcgc gctcaaccag gccttggcat
     1081 ccgccagcgc gatacccgtg tcggggtcgg gcagactcgc gtcggcaccg ccctggtact
     1141 tgtcccacac gccttgcagg gtcgagtact gcagcttgaa gtcgccgctg cgcgcgaccg
     1201 acagctcctg ttcgaggtgg gcgcggcgct cgtccgcgat ccgttcggcg tcggcctgcg
     1261 tgacctcgcc gagccgcgtc aggtacccga ggtacgcctc gcgcaccccc ttgcgactat
     1321 ggatcttcga gtacatcact ggctgggtga aggagggctc gtccgtctcg ttgtgaccga
     1381 agcggcggta ggcgtacatg tctaccacca cgtcgcgctt gaactcgctc ctgaagtcca
     1441 tggcgagctc gatgacctgc gcgacggcct ccgggtcctc gccgttcacg tggaacacgg
     1501 ggctctggag catcttcgcg atgtcggacg cgtacgtcgt gctgcggcct tggcgtggac
     1561 cggtcgtgaa gccgacctgg ttgttgacga tgatgtgcag cgcgccgccg actcggtaac
     1621 cgacgagccc gctcaagttg agggtctcct gcacgatgcc ctgccctatg aaggaggcgt
     1681 cgccgtggat gaggatgggc agtacgcgct cgcgccgcgc gtcgcccagg cggtcctgct
     1741 tggcgcgagc gcgcccgagt acgaccgtgt tgacgaactc gaggtgcgag gggttgaacg
     1801 cgagcgacag gtgcagacgc ttgccttgcg aggtcaccca gtcgttggag tagccgaggt
     1861 ggtacttgac gtcgcccctg cccaggtaga gctcggggtc cttgtcgtcg aactcgcgga
     1921 agatcgtgcg ggtgttcttg ccgaggatgt tcgcgagcac gttgaggcgg ccgcgatgcg
     1981 ccatgccgag cacgacctcc tcgacgccct gggcgccggc cttctcgatg gcgaggtcga
     2041 gtagggggat caacgtctcg ctcccctcga gcgagaagct cttggcgccg acgtacttct
     2101 tctgcaggaa ctcctcgaag atggtggcgt ccgtcagctt ggtgaggatg cgcagctgct
     2161 ggtcgcgcgg cagggcgagg cggttgcggg t
//
LOCUS       DAOQYE010000323         2191 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_57014_length_2191_cov_2.401685, whole genome shotgun sequence.
ACCESSION   DAOQYE010000323 DAOQYE010000000
VERSION     DAOQYE010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57014_length_2191_cov_2.401685"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            191..1645
                     /gene="trpE"
                     /locus_tag="PKN52_06040"
     CDS             191..1645
                     /gene="trpE"
                     /locus_tag="PKN52_06040"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HNQ99540.1"
                     /translation="MPTTRTGEGAASHRAPIEPIYREVLADLETPLTAYLKLAGHPSF
                     LLESVEQGERVARYSFIGTGQRLRIEARGDQVTLTRPTDTRTVTSADPLRVMWDEVVR
                     ETRPAPDLPSFWAGAVGYASYDLVRQYERLPDANPDVIGAPDMLFVEPELVVVFDHLK
                     RRLFIVAPAEVGNAADAARASALVDAAYERLRGPLPGVPGDRAGRLVEFESSLSREAY
                     MDAVARAVEYIHAGDVFQVVPSVRLSADVGAHPFAIYRALRTINPSPYLGYLDLGGIT
                     LVASSPESLVRSDGLKVETLPIAGTRPRGADAVRDEELATELLADEKERAEHVMLVDL
                     GRNDLGRVCAPGSVEVVDLMRVERYSHVMHIVSEVVGRLADGRTPLDALSATLPMGTA
                     SGAPKVRAMEIIDELETVRRGPYAGAFGYLAAGGAMDMALTLRTMVVKDGRIHLQAGA
                     GVVADSDPATEWQECMNKLAALRRAVEMATKGLG"
     gene            1645..>2191
                     /locus_tag="PKN52_06045"
     CDS             1645..>2191
                     /locus_tag="PKN52_06045"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HNQ99541.1"
                     /translation="MAALKLLVVDNYDSFTYNLVQYVAALGADVTVWRNDTFEVADVD
                     ALAPDGVIVSPGPCTPREAGVSIELVRELGAHLPMLGVCLGHQVIGEAYGGRVVRANR
                     LVHGKTAAIEHDGTGVFAGLESPVTATRYHSLVVVDPPGELVPNAWATEPGTGERVIM
                     GLRHATHPVWGVQFHPESILTQ"
BASE COUNT          332 a          821 c          720 g          318 t
ORIGIN      
        1 gccagctctt ggcgcgcccg ctcgcaagca cggaggcttg aggctatact cacccgcatg
       61 atccccctcc accgtaggac gggaaccacc tggcgatggc ggcggcactg aacctgccgt
      121 ccagcacgcg gccgcccatg ccctgacggg cgccgctcca agccagatgc gtccacgaac
      181 ggagtcgagc atgcctacaa ccagaacggg tgagggcgcc gctagccacc gcgcgcccat
      241 cgaacccatc taccgcgagg tcctggcgga cctcgagacg cccctcaccg cctacctcaa
      301 actggccggc cacccgtcct tcctcctcga gagcgtcgag cagggcgagc gcgtcgcgcg
      361 ctactcgttc atcggcaccg gacaacggct gcgcatcgag gcccgaggcg atcaggtgac
      421 cctgacgcgc cccacggaca cccgcaccgt gacgagcgcc gacccactgc gcgtcatgtg
      481 ggacgaggtg gtgcgcgaga cccgccctgc gccggacctg ccctcgttct gggcaggcgc
      541 ggtcggctac gccagctacg acctcgtgcg ccagtacgag cgcctgccgg atgccaaccc
      601 cgacgtcatc ggcgcgcccg acatgctctt cgtcgaaccc gagctggtcg tcgtgttcga
      661 ccacctcaag cgccggctgt tcatcgtcgc ccccgcagag gtgggcaacg ccgccgacgc
      721 cgccagggcc tctgcgctcg tcgacgccgc ttacgagcgg cttcgcggac cgctgcccgg
      781 cgtgcctggc gaccgcgccg ggcgcctcgt ggagttcgag tccagcctgt cgcgggaagc
      841 gtacatggac gccgtggcgc gcgccgtcga gtacatccac gccggcgacg tcttccaggt
      901 cgtcccgagc gtccgcctct ccgccgatgt cggtgctcac ccgttcgcga tctaccgcgc
      961 gctgcgcacc atcaacccca gcccgtacct cggctacctc gatctgggcg gtatcaccct
     1021 cgtggccagc agccccgaga gcctcgtgcg ctccgacggc ctgaaggtcg agaccctgcc
     1081 catcgccggt acgcgcccgc gcggcgccga tgccgtccgc gacgaggagc tggcgaccga
     1141 gctgctggcg gacgagaagg agcgcgccga gcacgtcatg ctcgtcgatc tgggccgcaa
     1201 cgatctcggc cgcgtctgcg cccccgggag cgtcgaggtc gtcgacctca tgcgcgtgga
     1261 acggtacagc cacgtcatgc acatcgtctc agaggtcgtc gggcggttgg cggacggccg
     1321 cacgcccctc gacgccctct ccgccaccct gccgatgggt accgcctccg gcgctccgaa
     1381 ggtacgcgcc atggagatca tcgacgagct cgagaccgtg cggcgcggac cgtacgccgg
     1441 cgccttcggt tacctcgcgg cgggcggcgc catggacatg gcgctgaccc tacgcaccat
     1501 ggtcgtcaag gacggccgca tccacctgca ggccggggcc ggcgtggtcg ccgactccga
     1561 ccccgccacc gaatggcagg agtgcatgaa caagctcgcg gcgctcaggc gcgcggtcga
     1621 gatggcgacg aaagggctgg gctgatggcc gccctcaagc tccttgtggt cgataactac
     1681 gactctttca cctacaacct cgtccagtac gttgcggcgc tcggtgccga cgtgaccgtg
     1741 tggcgcaacg acaccttcga ggtggccgac gtcgacgcgc tggccccgga cggggtgatc
     1801 gtctcgcccg gaccgtgcac gccgcgtgaa gccggggtgt cgatcgagct cgtacgagaa
     1861 ctcggcgcgc acctgccgat gttgggtgtg tgcctcggcc atcaggtcat cggcgaggcc
     1921 tacggtggcc gcgtcgtgcg ggccaaccgc ctcgtgcacg gcaagaccgc cgccatcgag
     1981 cacgacggca cgggcgtgtt cgcgggtctc gagagcccgg tcacggccac gcgctaccat
     2041 tcgctcgtgg tcgttgaccc gccaggggag ctcgtgccca acgcatgggc gaccgagccc
     2101 ggtaccggcg agcgcgtgat catgggcttg cgccacgcca cgcacccagt gtggggcgtg
     2161 cagttccacc ctgagagcat cctcacccag a
//
LOCUS       DAOQYE010000324         2190 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_57051_length_2190_cov_2.158314, whole genome shotgun sequence.
ACCESSION   DAOQYE010000324 DAOQYE010000000
VERSION     DAOQYE010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57051_length_2190_cov_2.158314"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..843
                     /locus_tag="PKN52_06050"
     CDS             <1..843
                     /locus_tag="PKN52_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehypoxanthine futalosine cyclase"
                     /protein_id="HNQ99542.1"
                     /translation="CLPFDWYLDLMRHLKERHPTIRVEAFSPEEIKGMAGLTGMSTRE
                     VLAALQAAGLDGLPGGCGEMLVDDIRHAKHVSPGRISADEWIRVMGEAQEFGLYTTAT
                     MVIGFGETHAQRVASLMRVRDQQDRAAATHGNGFSAFISWTLQTEGVRVHGKVPGAGA
                     HEYLQNVAVSRLLLDNVVNFQASWPTMGYKVAQTALYFGCNDFGSTMLEENVVSQAGA
                     KHSNTPESEIVRQIVDAGYRPAQRDSLYNIIRRVDPSEFDLPALPSTPSGQPRAAAEA
                     VTAG"
     gene            843..2027
                     /locus_tag="PKN52_06055"
     CDS             843..2027
                     /locus_tag="PKN52_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNQ99543.1"
                     /translation="MPLRPLTEVVGRQDPAYGRALAHAEVVYDGLGLPRAAAAVTVQL
                     AQGLASVVGVGNFAEARATNGAARVVDCGFAISPPVVNAHTHLDLSGMPHTPGSYADF
                     IAAVVAHARAGHRGSAAAKRGVTELSAAGTRVVGDVVTDAATMEYLLGQAELTGVAYW
                     EVFAPRPEDADAAFEAALAAVARFRTLERPGGMRVGLSPHTPHTVSAPLLVKLAAYAR
                     AERIPLAIHVAESPAELELHLKGTGELAESLARAGFGLAPTGVSPVRYLHDLGVLAAS
                     PAVIHAVQVDDDDARLLARAGAVVVHCPRSNAALECGAFPWTLFAKHGVEVAFGTDSR
                     GSSPDLDVTREVAAALVAQGGKLNPRAAVRAAVKGGHKALGMAPPRVVRGAPAGELVA
                     WL"
BASE COUNT          316 a          804 c          764 g          306 t
ORIGIN      
        1 tgcctgccgt tcgactggta cctcgacctc atgcggcacc tcaaggagcg gcaccctacc
       61 atccgcgtcg aggccttcag ccccgaagag atcaagggca tggccggcct caccggcatg
      121 agcacgcgtg aggtgctggc ggccctgcag gccgcgggcc tcgacggcct gcccggcggc
      181 tgcggcgaga tgctggtcga cgatatccgc cacgccaagc acgtgtcgcc agggcgcatc
      241 tcggccgacg aatggatccg cgtcatgggc gaggcccagg agttcggcct gtacacgacg
      301 gccacgatgg tgatcggctt cggggagacc cacgcccagc gcgtcgcgag cctcatgcgc
      361 gtgcgcgacc aacaggaccg ggccgccgcc acgcacggca acggcttctc cgcgttcatc
      421 tcatggactc tgcagacgga aggggtgcgc gtccacggca aggtccccgg cgccggggct
      481 cacgagtacc tgcagaacgt cgccgtcagc cgactgttgc tggacaacgt cgtcaacttc
      541 caggcttcct ggcccaccat gggctacaag gtggcccaga cggccctcta cttcggctgc
      601 aacgacttcg gctcgaccat gctcgaggag aacgtggtat cgcaggcggg cgccaagcac
      661 agcaacacgc cggagagcga gatcgtcagg cagatcgtcg acgccggcta ccgcccggcc
      721 cagcgcgact cgctctacaa catcatccgg cgcgtcgacc cgagcgagtt cgatctgccc
      781 gccctgccaa gcacgccctc cgggcagccg cgcgcggccg ccgaagccgt cacagcgggc
      841 tgatgccgct ccggccgctg actgaggtcg tcggccggca agaccccgcg tacgggcggg
      901 cgctggccca cgcggaggtc gtttacgacg gcttgggtct gccgcgcgcc gccgcggcgg
      961 tgaccgtgca actcgctcag gggctggcca gcgtcgtcgg cgttggcaac ttcgccgagg
     1021 cgcgtgcgac caacggcgcg gctcgggtcg tcgactgcgg tttcgcgatc tcgccgcccg
     1081 tcgtcaacgc ccacacgcac ctcgacctct caggcatgcc gcataccccc ggctcgtacg
     1141 ccgacttcat agccgccgtg gtggcccacg cgcgcgccgg ccaccgtggt tcggcggccg
     1201 ccaagcgggg cgtgacggag ctcagcgccg ccggcacgcg cgtggtcggc gacgtcgtga
     1261 cggacgccgc caccatggag tacctgctcg gccaggcgga gctcaccggc gtcgcgtact
     1321 gggaagtctt cgcgccgcga ccagaggatg ccgacgccgc gttcgaggcg gcgctcgcgg
     1381 ccgtcgcgcg cttcaggaca ctggagcggc caggcggcat gcgcgttggg ctctcgccgc
     1441 acacgccgca caccgtcagc gcccccctcc ttgtcaagct cgccgcgtac gcccgtgcgg
     1501 agcgcatccc tctcgccatc catgtggccg agagccccgc cgagctcgaa ctccacttga
     1561 agggcacggg cgagttggcc gagtcgttgg cgcgggccgg cttcgggctg gccccgaccg
     1621 gcgtgagccc cgtgcgctac ctgcacgacc tgggtgtcct cgcggcctcg ccggcggtca
     1681 tccacgccgt ccaggtcgac gacgacgacg cgcggttgct ggcgcgcgcc ggcgccgtgg
     1741 tcgttcactg cccccgctcc aacgccgcgc ttgaatgcgg agcgttccca tggacgctct
     1801 tcgccaagca cggcgtggag gtcgcgttcg gcaccgactc gcgcgggtcg agccccgacc
     1861 tcgacgtgac gcgcgaggtg gcggcggcgc tcgttgcgca gggcgggaag ctcaaccccc
     1921 gcgcggccgt gcgggccgcg gtcaagggtg ggcacaaggc gctgggcatg gcgccgccac
     1981 gcgtggtgcg cggcgctccg gccggcgagc tagtggcgtg gctctaggcg tgtcgagccc
     2041 gctacatgcg cgaggcggcg caggcaagta acctacgtgg catgcccggc gcgcgccggg
     2101 ggaggagact ggataacatg gccaactcgc tggaactcac cgacggcaac ttcgcctccg
     2161 agacggactc gggcctcgtc ctagtggact
//
LOCUS       DAOQYE010000325         2177 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_57562_length_2177_cov_2.495759, whole genome shotgun sequence.
ACCESSION   DAOQYE010000325 DAOQYE010000000
VERSION     DAOQYE010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57562_length_2177_cov_2.495759"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..811)
                     /locus_tag="PKN52_06060"
     CDS             complement(<1..811)
                     /locus_tag="PKN52_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[LysW]-aminoadipate kinase"
                     /protein_id="HNQ99544.1"
                     /translation="MSAPVVVKVGGSEGIDLNAVCDDVATMWRGGRRVVLVHGGSAET
                     NRVSTLLGVPPRFVTSPGGHQSRFTDRATLEVFEMVYCGKVNKGLVESLQGRGVNAVG
                     LSGLDGRLLEGRLKSTVRSVEDGRVLVLHGDHTGTVERVNAALLELLLDSGYLPVLTP
                     PAVSFEGVAMNVDGDRAAAAVAVALGAEALVLLSNVPGLLREFPDESTLVERVDAAAP
                     EAAMELAQGRMKKKVMGAVAAVQGGVGRVVLGDARAAEPVSRALRGRGTVVA"
     gene            complement(808..1872)
                     /gene="argC"
                     /locus_tag="PKN52_06065"
     CDS             complement(808..1872)
                     /gene="argC"
                     /locus_tag="PKN52_06065"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HNQ99545.1"
                     /translation="MSPVRVSILGASGYAGGEFLRLALGHPALEVSQVTSREHAGQPV
                     HIVHPNLRGVTDLRFCRPEELSNADVLVCALPHGGLADRVDDVMPLAGRVLVDLSDDF
                     RLTSPEAYARFHSRPHPRPDLLGTFVYANPELHRQELARTAASPAGPTRIAGAGCIAT
                     AAILALRPLVAEGLLDVARDVIADAKIGSSAAGKDVSLASHHPERSGAVRTYAPTGHR
                     HQAEIAQELAGGPTVHLTAAAVERVRGVLVAAHAFLRQGVAEADVLAAFRRAYDAEPF
                     VRLVRVRRGVHRVPDPRILDGSNYCDVGFALDEGTGRVVAMAALDNLVKGTAGHALQS
                     LNLALGFPEVAGLGFTGLHP"
     gene            complement(1970..>2177)
                     /locus_tag="PKN52_06070"
     CDS             complement(1970..>2177)
                     /locus_tag="PKN52_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysine biosynthesis protein LysX"
                     /protein_id="HNQ99546.1"
                     /translation="DELVERCLGAARAVGGGILAIDLVESSRGVLVVEVNHTMEFRNS
                     ITTTGVDIPGRIAAYAARQSELRA"
BASE COUNT          304 a          821 c          770 g          282 t
ORIGIN      
        1 acgccaccac cgtccctcgc ccgcgcagcg cccggctgac gggctcggcc gcgcgcgcat
       61 cgcccagcac gaccctgccg acgcctccct gcacggccgc caccgcaccc atgaccttct
      121 tcttcatccg cccctgcgcc agctccatgg cggcttcggg cgccgcggcg tccacgcgct
      181 ccaccagcgt cgactcgtcc gggaactctc gcagtaggcc gggcacgttg gagaggagca
      241 cgagcgcctc agccccgagc gccacggcga cggccgcggc ggcgcggtcg ccgtccacgt
      301 tcatggcgac cccctcgaag ctgacggcgg gcggcgtgag cactggcagg tagccgctgt
      361 cgaggagcag ctcgaggagg gcggcgttga cccgctcgac cgtgcccgta tggtcgccgt
      421 gcagaacgag cacgcggccg tcctcgacag acctgaccgt gctcttgagg cggccctcga
      481 ggagccgacc gtccagcccg gagagcccga cggcgttgac gccgcggccc tgcaggctct
      541 cgacgaggcc cttgttgacc ttgccgcagt agaccatctc gaagacctcc agggtcgccc
      601 ggtccgtgaa acggctctgg tgtccgcccg ggctcgtcac gaagcgcggg ggcacgccga
      661 gcagcgtcga gacgcggttc gtctcggcgc tgccgccgtg cacgagcacg acgcgccgtc
      721 cgccccgcca catggtggcg acatcgtcgc acacggcgtt caggtcgata ccttccgacc
      781 cgcccacctt cacgaccacc ggggcgctca cgggtgcaac cccgtgaagc cgaggccggc
      841 gacctcgggg aaaccgagcg ctaggttgag ggactgaagg gcgtggcccg ccgtgccctt
      901 cacgaggttg tcgagcgcgg ccatggccac gacgcgcccg gtgccctcgt cgagcgcgaa
      961 gccgacgtcg cagtagttcg acccgtcgag gatccggggg tccggcacgc ggtgcacgcc
     1021 gcggcgcacc cgcactaggc ggacgaacgg ctccgcgtcg taggcgcgcc tgaacgcggc
     1081 gagcacgtcc gcctcggcca cgccctgacg caggaacgca tgggcggcga ccagcacgcc
     1141 ccgcacgcgc tcgacggccg ccgccgtcag gtgcacggtg ggtcctccgg cgagttcctg
     1201 ggcgatctcc gcctgatgcc ggtgcccggt cggcgcgtag gttcgcacgg ctccggagcg
     1261 ctccggatga tgcgaggcaa gcgagacgtc cttgccggcc gcgctgctgc cgatcttggc
     1321 gtcggctatc acgtcgcgcg ccacgtcgag caggccctcc gccaccaacg ggcgcagggc
     1381 gaggatcgcc gcggtcgcga tgcaacccgc gcccgcgatc ctggtggggc cggcgggcga
     1441 agccgccgtc cgcgccagct cctggcggtg cagctccggg ttggcgtaca cgaacgtgcc
     1501 gagcaggtcg ggacgcgggt ggggacggct gtggaacctg gcgtacgctt cgggactcgt
     1561 caggcggaag tcatccgaga ggtcgaccaa cacgcgaccg gccaagggca tgacgtcgtc
     1621 caccctgtcg gcgagcccgc cgtgcggcag agcgcacacg agcacgtcgg cgttggacag
     1681 ctcctccggt cggcagaagc gcagatcggt gacgccgcgc aggttgggat ggacgatgtg
     1741 gaccggctga ccggcgtgct cccggctcgt cacctggctc acctcgaggg ccgggtggcc
     1801 gagcgccaac ctgaggaact ccccgcccgc gtagccggac gccccaagga tgctgacgcg
     1861 caccgggctc atgcgcccgg gtccatgcgc ccggtcgccc ccgccgtccc atcgcgccgc
     1921 gcccaagcgc cgcgcccgcg tgccgggccg tggcgcgttc cccgggggtt cacgcgcgca
     1981 gctccgactg acgggcggcg taggcggcga tccgccctgg gatgtcgacg ccggtggtcg
     2041 tgatggagtt ccggaactcc atcgtgtggt tgacctccac cacgagcact ccgcggctcg
     2101 actccaccag gtcgatggcg aggatgccgc cgccgaccgc acgcgcggcg ccaaggcagc
     2161 gctcgacgag ttcgtcc
//
LOCUS       DAOQYE010000326         2167 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_57957_length_2167_cov_2.652462, whole genome shotgun sequence.
ACCESSION   DAOQYE010000326 DAOQYE010000000
VERSION     DAOQYE010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57957_length_2167_cov_2.652462"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..834
                     /locus_tag="PKN52_06075"
     CDS             <1..834
                     /locus_tag="PKN52_06075"
                     /inference="COORDINATES: protein motif:HMM:NF016952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HNQ99547.1"
                     /translation="AVAEHGRLAGGLSSARPGRGRRVRSRPILAVAALALALLAAGCR
                     GQGVERLQVQVVAQHPHDPQAFTQGLVWSNGTLYESTGLLGRSSLREVKLDGTVLRRR
                     DLAPNLFGEGLELVGDELLQITWQNGVLLRYDVDTFEPKGTQRYEGEGWGLCFDGTDL
                     WMSDGSARLTRRDPVTFAAKGTVDVTSDGKPLTMLNELECVAGTIYANVWTTDEIVRI
                     DPGTGKVTAVIDATELRRAAGVTDRDAVLNGIAYDDASGNFLVTGKLWPALFEVRFVA
                     K"
     gene            complement(877..1188)
                     /locus_tag="PKN52_06080"
     CDS             complement(877..1188)
                     /locus_tag="PKN52_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family transcriptional regulator"
                     /protein_id="HNQ99548.1"
                     /translation="MPTYLYRNLDTGETFEYEQRITDAPLSVHPETGAAVKRLIQPVG
                     IAFKGSGFYVNDSKKSGASKLESKPAGSSDAKAADSATAPASSAPSATPAPAASSAAA
                     D"
     gene            1336..>2167
                     /locus_tag="PKN52_06085"
     CDS             1336..>2167
                     /locus_tag="PKN52_06085"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HNQ99549.1"
                     /translation="MTCAASPGLFTQGPLAVIGAGAWGTVVASLLARNGHEVRLWSHR
                     EALADAINAGGRNEEYVPGLALPAGVRATADLAAAVRGCVLAFMAVPSKAFRQVSERL
                     AHVGAPPALVSCSKGLEVATFKRLSQVAAEYLPGVSIAVVSGPNLATEIAADKPAATT
                     VASPDAGLAKGVQQLLQQRTFRAYTSADLIGVEMAGALKNVIALACGVSDGLGLGDNT
                     KASIITRGLAEIVRFGTAVGGQVATFYGLAGVGDLVATCSSAESRNHKVGVLLADGAT
                     L"
BASE COUNT          322 a          758 c          752 g          335 t
ORIGIN      
        1 gccgtagccg agcacggccg tctggcgggc ggtctctcct ctgctcgtcc cggacgaggt
       61 cgacgcgtga gatcgcgccc gatcctcgcg gtggcggctc tggcactagc actgctcgct
      121 gccggctgcc gcgggcaggg cgtcgagcgg ctgcaggtcc aggtcgtcgc ccagcatccg
      181 cacgacccgc aggcgttcac ccagggcctc gtgtggtcca acgggaccct gtacgagagc
      241 acggggctgc tcggccgctc cagcctgcgc gaggtgaagc tggacggcac ggtgctgcgc
      301 aggcgtgacc tcgcccccaa cctgttcggg gagggtcttg agttggtcgg cgacgagctt
      361 ctccagatca cgtggcagaa cggcgtcctg ctgcggtacg acgtcgatac gttcgagccg
      421 aagggtaccc aacgctacga gggcgagggc tggggcctct gcttcgacgg cacggatctc
      481 tggatgagcg atggcagcgc caggctgacc agacgcgatc cggtcacctt cgcggccaaa
      541 gggaccgtgg acgtgacgag cgacggcaaa cccctgacca tgctgaacga gctcgaatgc
      601 gtggccggca ccatctacgc caacgtctgg acgaccgacg agatcgtcag gatcgacccg
      661 ggcaccggca aagtaacggc tgtgatcgac gcgacggaac tgaggcgcgc ggccggagtg
      721 accgatcgcg acgcggtcct caacggcatc gcctacgacg acgcgagcgg caacttcctg
      781 gttacaggga agctgtggcc ggcgctgttc gaggttcggt tcgtcgccaa gtagccctag
      841 ccagccgcgg ctgccgcggc tgccgcggcg actgcgtcag tcggctgccg cgctgctggc
      901 ggcaggcgcc ggcgtcgcgc tcggcgcgct gctggccggc gcggtcgcgc tgtccgcggc
      961 cttggcatcg gagctgccgg caggcttgga ctcgagcttg ctcgctcccg acttcttgct
     1021 gtcgttgacg tagaaccccg agcccttgaa cgcgatcccg accggctgga tcaggcgctt
     1081 cacggccgcg cccgtctccg ggtggaccga cagcggggcg tcggtgatcc gctgctcgta
     1141 ctcgaacgtc tcaccggtat cgaggttccg ataaaggtaa gtgggcatgg ttcctccgag
     1201 gttctcgcgc cgccatgctg gtgcggcggg catcacttgg cccggcgacg aacgcgccca
     1261 cggcacgtcg tagcggacga gcgaccccat cgcggcgcca aaagccacga tagcaccacc
     1321 tggtacgctt atcacatgac ctgcgctgca tcccccggcc tcttcaccca aggccccttg
     1381 gccgtcatcg gcgcgggcgc ctggggcacc gtcgtcgcct cgctcctcgc ccgcaacgga
     1441 cacgaggtgc ggctctggtc ccaccgggag gcgctggccg acgccatcaa cgccggcggc
     1501 cggaacgagg agtacgtgcc tgggctggcg ctgccggccg gggtgcgcgc gaccgccgat
     1561 ctggccgccg ccgtgcgcgg ctgcgtacta gccttcatgg ccgtgccatc gaaggcgttc
     1621 aggcaggtca gcgaacggct ggcgcacgtc ggtgctccac cggcgctcgt gagctgctcg
     1681 aagggcctgg aggtcgccac gttcaagcgc ctctcccagg tagccgccga gtacctgcct
     1741 ggcgtgtcga tcgccgtcgt ctcggggcct aacctggcca ccgagatcgc cgccgacaag
     1801 cccgccgcca ccacagtcgc cagtcctgac gccgggctgg ccaagggcgt ccagcagctc
     1861 ctccagcagc ggactttcag ggcctacacg agcgccgacc tgatcggcgt cgagatggcc
     1921 ggggcgctca agaacgtcat cgcgctggcc tgcggcgtca gcgacggcct cggacttggc
     1981 gacaacacga aggcgtcgat catcacccgc ggtctggccg agatagtccg tttcggcacg
     2041 gccgtcggcg gacaggtcgc aaccttctac ggcctggcgg gcgtcggcga cctggtcgcc
     2101 acgtgctcca gcgccgagtc gcgcaaccac aaggtcggcg tgttgctcgc ggacggtgcg
     2161 acgctcg
//
LOCUS       DAOQYE010000327         2162 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_58184_length_2162_cov_2.457523, whole genome shotgun sequence.
ACCESSION   DAOQYE010000327 DAOQYE010000000
VERSION     DAOQYE010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58184_length_2162_cov_2.457523"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1599)
                     /gene="secD"
                     /locus_tag="PKN52_06090"
     CDS             complement(<1..1599)
                     /gene="secD"
                     /locus_tag="PKN52_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HNQ99550.1"
                     /translation="MKRNPVTGIIILALIVASILYLWKPWNTTEPALRLGLDLQGGLR
                     VTLVSDTPNPASEDLATARNIVENRVNQFGVSEAVVSTAGNDKVVVELPGLTTDDQQR
                     ALDLIGQQAVLEFRIVKSASNALPTESLILDDLEPVAFTGEIVSTASAQFQQVPGAPT
                     GPVVVFEIRAADAQAFGNFTGGNVGRRMAIVLDGRIITAPTLQSRISDSGQITGIGTL
                     DEASDIAVVLRTGSLPVSLKVDEVRSIGPTLGQDSINKGTTAAIVGSALVILAVLIYY
                     GPLFGGVLAFGVILAMLFIFGVLAGLGAALTLPGLAGLVLTIGSAVDGNVISFERIRE
                     ELREGRGLKTAMKRGFEHSLSAIIDANVTTILAALALYQYTTGPVRGFAITLAIGIVA
                     SVFVNVIVVPYVLGLLVGNGNRTYMWRGFEVRGIRFVENARYFVSITALLAIASLIYV
                     GIVGLKMSTDFTGGSSVQLEVPVSESTADIRAAIDALGFPGVSGESANIVEDADTSVT
                     GTRQVIVRVGLGDESQTSTDDFAER"
     gene            complement(1672..2034)
                     /locus_tag="PKN52_06095"
     CDS             complement(1672..2034)
                     /locus_tag="PKN52_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99551.1"
                     /translation="MRLLGPARYSRAWRLACGRLALACALALLPLPLHALAGRVAATA
                     TDTTTQSMGSPVPLIIVEGSARVPAPTFGLQLMLPPAPSRFAGRRPAAPIAAPPQSPR
                     AHAGDRLIGIGRLLLDGG"
BASE COUNT          350 a          670 c          826 g          316 t
ORIGIN      
        1 gcgctcggcg aaatcgtccg tggaggtctg ggactcgtcg ccgaggccga cacgcacgat
       61 gacctggcgc gtccccgtga ccgaggtgtc ggcgtcttcc actatgttcg ccgactcacc
      121 gctcacgccg gggaacccga gcgcgtcgat ggcggcgcgg atgtcggccg tcgactcgct
      181 gacgggcacc tccaactgca cgctggagcc gcccgtgaag tctgtggaca tcttgagccc
      241 gacgatcccg acgtagatga gcgaggcgat ggcgaggagc gccgtgatgg acacgaagta
      301 gcgcgcgttc tccacgaacc tgatgccgcg cacctcgaag ccgcgccaca tgtaggtgcg
      361 gttgccgttg ccgacgagga gcccgaggac gtacggcacc acgatgacgt tcacgaacac
      421 ggaggccacg atgccgatgg cgagggtgat ggcgaagccg cgcaccgggc cggtcgtgta
      481 ctggtagagg gcgagcgcgg cgaggatggt ggtgacgttg gcgtcgatga tggccgagag
      541 cgagtgctcg aagccgcgct tcatggcggt cttcaggccg cgcccctcgc gcaactcctc
      601 gcggatgcgc tcgaacgaga tcacgttgcc gtcgaccgcc gagccgatgg tgagcacgag
      661 gccggcgaga cccggcagcg tcagggcggc gccgaggccc gcgaggacac cgaagatgaa
      721 gagcatggcg aggatgacgc cgaaggcgag cacgccgccg aacagcgggc cgtagtagat
      781 gaggacggcg aggatcacga gggcgctgcc gacgatggca gcggtcgtgc ccttgttgat
      841 ggaatcctga ccgagcgtcg gcccgatgga gcggacctcg tcgaccttca ggctgaccgg
      901 caggctgcca gtgcggagca cgacggcgat gtcgctcgcc tcgtctaggg tgccgatgcc
      961 cgtgatctgc ccggagtcgg agatacgcga ctggagcgtc ggcgccgtga tgatgcggcc
     1021 gtcgaggacg atggccatgc gcctgccgac gttgccgccg gtgaagttgc cgaacgcctg
     1081 cgcgtcggca gcgcggatct cgaagacgac gaccgggccg gtcggcgcac ccggcacctg
     1141 ctggaactgc gcgctggcgg tggagacgat ctcgccagtg aacgcgacgg gctcgaggtc
     1201 gtcgaggatc agcgactcgg ttggcagggc gttggaggcg gacttgacga tgcgaaactc
     1261 gagcacggcc tgctggccga tgaggtcgag cgcgcgctgc tggtcgtcgg tcgtcaggcc
     1321 agggagctcg acgacgacct tgtcgttgcc ggccgtggag accacggctt cggacacgcc
     1381 gaactggttg acgcggttct cgacgatgtt gcgggcggta gcgaggtcct cgctcgcggg
     1441 gttcggcgtg tcggagacga gggtgacgcg caagccgccc tgcaggtcga ggccgagcct
     1501 gagggcgggc tccgtggtgt tccacggttt ccacaggtag aggatcgagg cgacgatcag
     1561 cgccaggatg atgatgcccg tgacggggtt acgtttcatg cgactccaaa ggttggcttg
     1621 ggtgaggtgg tgggtagggt gcggacgtca ggtcaggtcg ctcgcggggc ctcaaccgcc
     1681 gtccagcagc agcctgccga taccgatgag cctgtcgccc gcgtgtgccc gcggggactg
     1741 tggcggcgcg gcgatgggcg cggcaggtcg gcggccggcg aagcgcgacg gcgcgggcgg
     1801 cagcatgagc tgcagcccga acgtcggagc gggcacccgc gcgctcccct cgacgatgat
     1861 gagggggacg ggcgagccca tgctctgcgt cgtggtgtcg gtagccgtgg ccgcgacccg
     1921 tccggcgagc gcgtgcagcg ggagcggcag gagcgcgagc gcgcacgcga gggccaggcg
     1981 gccgcaagcg agccgccaag ccctcgagta gcgcgccggc ccgagcagcc gcatcagacc
     2041 gcggacacct cgaccatctc gtagacctcg atgaggtcgc cctcctggac gttgtcgtag
     2101 ttctggaggc tgataccgca ctcgtagccg gtgccgacct cgcgcacgtc gtccttgaag
     2161 cg
//
LOCUS       DAOQYE010000328         2161 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_58211_length_2161_cov_3.114435, whole genome shotgun sequence.
ACCESSION   DAOQYE010000328 DAOQYE010000000
VERSION     DAOQYE010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58211_length_2161_cov_3.114435"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="PKN52_06100"
     CDS             <1..367
                     /locus_tag="PKN52_06100"
                     /inference="COORDINATES: protein motif:HMM:NF024732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HNQ99552.1"
                     /translation="SALEIDLTGQVCADSLGTFQYSGVGGQMDFIRGASLSERGKPII
                     ALPSVTSRGESRIVPYLKQGAGVVTTRAHVNYVVTEHGAASLHGKNLRQRARALIGIA
                     HPGHREALEKAAWERFGRL"
     gene            complement(432..1046)
                     /locus_tag="PKN52_06105"
     CDS             complement(432..1046)
                     /locus_tag="PKN52_06105"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HNQ99553.1"
                     /translation="MTSLIAGSPEAYLGADPIVESTHSDVVALATALRSSNPEEEAFA
                     GAAFAWVRDNVAHSWDARDPRVTLTASQVLREKVGLCYAKSHLLAALLRAGGVPAGLC
                     YQRLRDGDGHVLHGLIAVYLRGGWHRQDARGNKPGVEARFSLDGEQLAYVVDADAGEV
                     DYPEVHVSPVRPVVEVLGGADDVLALCEGGLPTRLEAVESGRGA"
     gene            complement(1190..>2161)
                     /locus_tag="PKN52_06110"
     CDS             complement(1190..>2161)
                     /locus_tag="PKN52_06110"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNQ99554.1"
                     /translation="ARLGGAVRFVGRVGADPFGGRLAAALEHDGVDTAALGRSTRPSG
                     VAFIQVDEHGQNTIVVSPGANHDLSVADLGAGEAGASLATATATVLMLQLEIPLDVVL
                     AAAAAGRRAGALVMLNLAPARRLEPSQLADVSLLLVNEHEAAIQLGADAEGKTPEEWA
                     VALCRFVPSAVVTLGGDGAVWACRAGPADADGSRAVSGDGGASSRSEGWSGGVSHGRV
                     PAFEVTVIDTTAAGDAFAGALAYWLAQAGLLAGRVGGVSETSGSAPPGVPSGATHLAE
                     RGGEHPLAAAVRFANAAGALAATRPGAQPSLPTLADVTDMLRRRLLE"
BASE COUNT          321 a          837 c          708 g          295 t
ORIGIN      
        1 ctcggccctc gagatcgacc tgaccgggca ggtctgcgcc gactcgctgg gcaccttcca
       61 gtactcggga gtgggcggcc agatggactt catccgcggc gcctccctgt ccgagcgcgg
      121 caagccgatc atcgcgctgc cgtccgtcac ctcgcgcggg gagagccgga tcgtgcccta
      181 cctaaagcag ggcgccgggg tggtgacgac gcgcgcgcac gtcaactacg tcgtcaccga
      241 gcacggcgcc gcctcgctgc acggcaagaa cctgcggcag cgggcgcgcg cgctcatcgg
      301 catagcgcac ccggggcacc gcgaggcgct ggagaaggcg gcctgggagc gcttcgggcg
      361 gctctaggcc gggggcgctt cggcagctct aggctgcaag cgcgacgctt ccgatctcgt
      421 gccagatgat gtcaggcgcc tcgtcccgac tccaccgcct ctagcctcgt cggcaggcca
      481 ccttcgcata gcgccaacac gtcgtcggcc ccgccgagca cctccacaac ggggcggacc
      541 ggcgacacat gcacctccgg gtagtcgacc tcccccgcat cggcgtcgac cacgtacgcc
      601 aactgctcgc cgtccagcga gaaacgcgcc tctacccccg gcttgttccc tcgggcatcc
      661 tgacggtgcc accctccgcg gaggtacacg gcgatgaggc cgtgcagcac gtgtccgtcg
      721 ccgtcccgca gccgctggta gcacagcccc gccggcacgc ccccggcgcg cagcagcgcc
      781 gccaggaggt gcgacttcgc gtagcagagg cccaccttct ctcgcaacac ctgggaggcg
      841 gtgagcgtca cccgggggtc ccgcgcgtcc cacgaatgcg ccacgttgtc ccgtacccac
      901 gcgaaggccg cgcccgcgaa ggcctcttct tcggggttgg aggagcgcag cgccgttgcg
      961 agggcgacga cgtcggagtg cgtcgactcg actatcgggt cagcccctag gtacgcctcc
     1021 ggcgagccag cgatcagcga tgtcatcgct acttcttacc accaggcttc gcccaagccc
     1081 cgccctccgc tttaccctcg cctcgccgtc cgccatgagg gccgacgcga tccggtaccg
     1141 cggcacccgg tcctcgcggc ccgcaagcca ggcctttcac ttcaggcctt cactccagga
     1201 gccttctccg aagcatgtcg gtcacatctg ccagggtcgg tagcgacggc tgcgcaccag
     1261 ggcgggtagc ggccagggcg ccggcggcgt tggcgaacct gacggctgcc gcaagagggt
     1321 gctcgccgcc gcgctccgcc aagtgcgtcg cgcccgacgg aacgccgggc ggagcagaac
     1381 cgctggtctc agacacgccg ccgaccctcc cggccaagag cccggcctgc gccagccagt
     1441 aggcgagcgc cccggcgaag gcgtcccccg ccgccgtcgt gtcgatcacg gtcacctcga
     1501 acgcgggcac acgaccatgt gatacgcctc ccgaccaccc ttccgaccgg gagctagctc
     1561 ccccgtcccc actcacagca cgacttccgt ctgcgtcggc cgggcctgct cggcaggccc
     1621 acacggcccc gtcgccccct agggtgacaa cggcgcttgg cacgaaccgg cagagggcga
     1681 cggcccactc ctcgggcgtc ttcccttccg catcggcccc cagttggatg gcagcctcat
     1741 gctcgttcac gagcagcaag ctcacgtcgg ccagttgact cggttcgagc cgccttgcag
     1801 gggcgaggtt gagcatgacg agcgcgcccg cccgccgacc cgcggccgcg gcggccagga
     1861 cgacgtcgag cgggatctcc aactgcagca tgaggacggt cgcggtcgcg gttgcgagcg
     1921 acgcgcccgc ctcgcctgcg cccaggtcgg caacgctcaa gtcgtggttc gctccgggtg
     1981 agacgacgat ggtgttctgc ccgtgctcgt caacctggat gaacgccacg ccgctcgggc
     2041 gggtactccg accgagggcg gcggtgtcca cgccgtcatg ctcgagggcc gccgcgagcc
     2101 gcccgccgaa cgggtcagcg ccaacgcgac ccacgaaccg cacggcgccg ccgagccgag
     2161 c
//
LOCUS       DAOQYE010000329         2160 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_58253_length_2160_cov_3.021378, whole genome shotgun sequence.
ACCESSION   DAOQYE010000329 DAOQYE010000000
VERSION     DAOQYE010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58253_length_2160_cov_3.021378"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..778)
                     /locus_tag="PKN52_06115"
     CDS             complement(<1..778)
                     /locus_tag="PKN52_06115"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNQ99555.1"
                     /translation="MKGLILAAGLGTRLRPITSLKPKPTIAVANKPLIHHAVDNLVEA
                     GVREIGVVVSYLTLNSIKETLDGYPGVRFEYIMQNPPQGLAHAVKVSREFLGDEPFVM
                     YLSDNLFEHGITEFVNRFRPGESNAVMALVPVSLEAAKSLGVAVVEGERITKLVEKPS
                     DPPSTLAVAGVYVFDSRIHAMIEGLAPGAKGEYQITDAIQRLIEAGDEVVPVTVRGWW
                     KDTGQPDDILDANRLLLSRLKRDIKGTIENANLIGEVVIGA"
     gene            911..1264
                     /locus_tag="PKN52_06120"
     CDS             911..1264
                     /locus_tag="PKN52_06120"
                     /inference="COORDINATES: protein motif:HMM:NF040082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99556.1"
                     /translation="MLKSSHIRLAVDMDDTGIEAIRWQADDSPEPGENDAGAMILSLW
                     DGAQRNALRIDLWVKEMSVDDMNDFFFQTFISMADTYRKATNDAGLASEIKLFARDFA
                     EKASRAAQAGGAQKA"
     gene            1330..>2160
                     /locus_tag="PKN52_06125"
     CDS             1330..>2160
                     /locus_tag="PKN52_06125"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNQ99557.1"
                     /translation="MVVRTARWTSGPGRLELGRTLPSLLAEAVRERPNERAFNDRPSA
                     VPGKPGGPGTPWTSLSNSEFKLRAEAMAAGLRSMGLERGDRVALLTHSDMSFVLADMA
                     CLIAGLIDVPIYLTHTRDAMRFILQESACAAVLTSDTVLLAQALDVTSDLPGVKLVAV
                     ASGGLASGGEVESADGDGGTLVTDYATVIAMGERRLAERPGLIESFAASQQASDVATI
                     IYTSGTTGSPKGVMLSHENLSSNAIASLTDLPTFLSGDQETVLSFLPLTHIFARTLMY
                     A"
BASE COUNT          345 a          712 c          733 g          370 t
ORIGIN      
        1 ctgccccgat gactacctcg ccgatgaggt tggcgttctc gatggtcccc ttgatgtctc
       61 gcttcaagcg ggagaggagc agccggttgg cgtccaggat gtcgtccggc tgacccgtgt
      121 ccttccacca gccgcgaacg gtgacgggca cgacctcgtc tcccgcctcg atgaggcgct
      181 ggatggcgtc ggtgatctgg tactcgccct tggcgccggg ggccaggcct tcgatcatgg
      241 cgtggatgcg cgagtcgaac acgtacacgc cggccacggc cagggtgctg ggtgggtcgc
      301 tcggcttctc gacgagcttg gtgatgcgct cgccctccac caccgcgacg ccgagcgact
      361 tggcggcctc caggctcacc ggtacgaggg ccatgacggc gttgctctcg cccggcctga
      421 agcggttcac gaactcggtg atgccgtgct cgaagaggtt gtcggagagg tacatgacga
      481 acggctcgtc gccgaggaac tcgcgcgaga ccttgacggc gtgtgccaga ccttgcggcg
      541 ggttctgcat gatgtactcg aagcgcacgc ccgggtagcc gtccagcgtc tccttgatgg
      601 agttgagcgt caggtagctg accacgacgc cgatctcgcg cacgccggcc tcgacgaggt
      661 tgtccacggc gtggtggatg agcggcttgt tggctaccgc gatcgtcggc ttcggcttga
      721 ggctggttat cggacgcagg cgggtgccga gaccggctgc gagaatgagt cccttcaaga
      781 ggccctccat ggcgggcggc gaccgcgggc gtccctagcc gtgtagcgct tcctaccggg
      841 tcgcaccggc gccgggtaac gccatccaag ataccgtgtg caccgccgcc ggtgcagtta
      901 ccatgctcgc atgttgaagt cttcccacat ccgcctcgcg gtcgacatgg acgacacggg
      961 catcgaggcc atccgctggc aggccgacga ctcccccgag ccgggcgaga acgacgccgg
     1021 ggccatgatc ttgtcgctgt gggacggtgc tcagcgcaac gcactgcgca tcgacctctg
     1081 ggtcaaggag atgtcggtcg acgacatgaa cgatttcttc tttcagacgt tcatctccat
     1141 ggcggatact taccgcaagg caacgaacga cgccggcttg gcgagtgaga taaagctgtt
     1201 cgcgagggac ttcgccgaga aggcgtcgcg cgcggcgcaa gccggaggtg cccagaaggc
     1261 ttgagcgctc acgtggtgtg agcgttcacc tggcgtgagc gcctccgagc cgccgccctg
     1321 gaggcaagga tggtcgtgag aacggcacgg tggacgtcag gacctgggcg tctggagttg
     1381 ggccgcacgc tgccgtcgtt gctcgccgag gccgtgcggg agagaccgaa cgagcgcgcg
     1441 ttcaacgacc gcccgtcggc cgtgcccggc aagcccggcg ggccgggcac gccttggacc
     1501 agcctatcga acagcgagtt caagctccgg gccgaggcca tggccgccgg tctacggtcc
     1561 atgggcttgg agcgcggtga ccgcgtcgcg ctgctcacgc actcagacat gagcttcgtg
     1621 ctggccgaca tggcctgcct gatcgcaggg ctcatcgacg tgcccatcta cctaacgcac
     1681 actcgcgacg ccatgcgctt catcctgcag gagagcgcct gcgcggcggt cttgacgtca
     1741 gacaccgtgc tgctcgccca ggcgctcgac gtcacgagcg acctaccggg cgtgaagctc
     1801 gtggcggtgg cgagcggcgg cttggcgtca ggcggcgaag ttgagtccgc ggacggcgac
     1861 ggcggcacgc tggtgaccga ctacgcgacc gtcatcgcca tgggagaacg gcggctggcc
     1921 gaacgccccg ggttgataga gtccttcgcg gcctctcagc aggcgagcga cgtggccacg
     1981 atcatctaca cctccggcac gaccggctcg ccgaagggcg tgatgctcag ccacgagaac
     2041 ctctcgagca acgccatcgc ctcccttacg gacctgccga cgttcctctc cggcgaccag
     2101 gagactgtgc tctccttcct gccgctcacg cacatcttcg cgcgcaccct catgtacgcg
//
LOCUS       DAOQYE010000330         2160 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_58262_length_2160_cov_2.415202, whole genome shotgun sequence.
ACCESSION   DAOQYE010000330 DAOQYE010000000
VERSION     DAOQYE010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58262_length_2160_cov_2.415202"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..968)
                     /locus_tag="PKN52_06130"
     CDS             complement(<1..968)
                     /locus_tag="PKN52_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HNQ99558.1"
                     /translation="MSSDPVDPPSTAKPPPRGLLERIDQPADLKLLQPTQLPELAEEL
                     RSEVIRVCSVAGGHLASSLGAVELTVALHYLFDTRRDRIVWDVGHQTYGHKILTGRKD
                     RIETIRTGGGLAGFTSVSESEHDALTVGHASTSLAAALGMALARDARGDDYEVAAVIG
                     DGALTGGMALAALNQIGHLKPRMLIVLNDNEMSISENVGGINHYMRTLQVQPWFQRAE
                     DRAKARLVRFSEPLAELGSRAKKAARRFFDPASNNPFHAMGLRYVGPIDGHDIPQLLY
                     YLAKIRELDGPTMLHIVTRKGKGYKVAESDPITWHGASAFDPDHPVA"
     gene            complement(981..1736)
                     /locus_tag="PKN52_06135"
     CDS             complement(981..1736)
                     /locus_tag="PKN52_06135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99559.1"
                     /translation="MPSATRFTPGRSERTTGRPPVQVARVGALIGLVALLLAACAPSG
                     ADAPRVGVEQLTEPISFYPNQTGATWEYLPNGARLTDPRTSVQVVGPTIVNGEVWVAW
                     HARGRGLDEMSYRQVRADGVWLKRQERLGTTYTFDPPLQEYPPEGQLRVGGLWTGKTT
                     VHISADGGKQELDMAVDYTYMVVDKRKVTVPAGDIEVYVIDFISRTIDENGAVTEELT
                     QQSWFAPYVGDVRLRSNQVLVSTNVPSVAPEEP"
     gene            complement(1848..>2160)
                     /locus_tag="PKN52_06140"
     CDS             complement(1848..>2160)
                     /locus_tag="PKN52_06140"
                     /inference="COORDINATES: protein motif:HMM:NF014856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylglycinamide synthetase C
                     domain-containing protein"
                     /protein_id="HNQ99560.1"
                     /translation="LGSAVAEWRDAYSACVVMAAPGYPTSPVGGVPVAVPAELPDDVM
                     VFAGGLTPAGRPGEYVTSGGRALNVVGTGATAARARAAAYAAVEQISFPGAQYRTDIG
                     R"
BASE COUNT          308 a          706 c          787 g          359 t
ORIGIN      
        1 gccaccgggt ggtcggggtc gaaggccgag gcgccgtgcc aggtgatggg gtcgctctcc
       61 gccaccttgt agcccttgcc cttgcgcgtg acgatgtgca gcatggtggg cccgtcgagc
      121 tcccttatct tggcgaggta gtagaggagc tgcgggatgt cgtgaccgtc gatggggccg
      181 acgtagcgca ggcccatggc gtggaacggg ttgttgctgg cagggtcgaa gaagcgcctg
      241 gccgccttct tcgcgcggct gccaagctcc gccagcggct cggagaagcg cacgaggcgg
      301 gccttggcgc ggtcctccgc ccgttggaac cagggttgga cctggagggt gcgcatgtag
      361 tggttgatgc cgccgacgtt ctcgctgatc gacatctcgt tgtcgttgag cacgatgagc
      421 atgcgcggct tcaagtggcc gatctggttg agcgccgcga gcgccatacc gccggtcagc
      481 gccccgtcgc ctatgactgc ggcgacttcg taatcgtcgc cacgggcgtc gcgggcgagc
      541 gccatcccca gcgccgcggc gaggctggtc gacgcatggc cgacggtgag cgcgtcgtgc
      601 tccgactcgc tcacggacgt gaagccggcg aggcccccgc ccgtgcggat cgtctcgatc
      661 cggtccttcc tccccgtgag gatcttgtga ccgtacgtct ggtggccgac gtcccacacg
      721 atgcggtcac ggcgcgtgtc gaacaggtag tgcagcgcga cggtcaactc gaccgcccct
      781 aggctcgagg ccagatggcc cccagctaca gagcagacgc ggatgacttc ggagcgcaac
      841 tcctcggcga gttccggcaa ctgggtcggc tggaggagct tcaggtcggc cggctgatct
      901 atccgttcca acaaccccct gggcggaggc ttggcggtgg agggagggtc gacggggtcg
      961 gaactcatgg cgttaccgac tcagggctcc tcaggcgcta cggacggcac gttcgtgctg
     1021 accagcacct ggttggagcg cagccgcacg tcgccgacgt agggggcgaa ccacgactgc
     1081 tgcgtgagct cttccgtgac ggcgccgttc tcgtcgatgg tgcggctgat gaagtcgatg
     1141 acgtagacct cgatgtcgcc ggcaggcacg gtgaccttgc gcttgtccac caccatgtag
     1201 gtgtagtcga cggccatgtc gagttcctgc ttgccgccgt cggcgctgat atggacggtg
     1261 gtcttgccgg tccagaggcc gcccacgcgc agttggccct ccggcgggta ctcctgtagc
     1321 ggcgggtcga acgtgtaggt cgtgccgagg cgctcctggc gcttgagcca cacgccgtcc
     1381 gctcggacct ggcggtagct catctcgtcg agcccgcggc cacgcgcatg ccaggccacc
     1441 cacacctcgc cgttgacgat ggtggggccg acgacctgca ccgacgtgcg agggtcggtg
     1501 aggcgcgcgc cgttcgggag gtactcccag gtggcgcccg tctggttcgg gtagaaggag
     1561 atgggttcgg tcagttgctc gacgccgacg cgcggggcgt ccgcgccgct cggcgcgcag
     1621 gcggctagga ggagcgcgac gaggccgatt agagcaccga cgcgggctac ctgaacgggt
     1681 ggacgaccgg tcgttcgttc gctgcgaccg ggcgtgaacc gagtggcgct aggcatgcgt
     1741 cgagtttacc gcgtcggggg ttcaggaagc gctcgtgaca tcctccacta gcaatttgct
     1801 agtgcgcggg cgcactagca gttggccggc catcttcccg gcggcgctca ccgcccgatg
     1861 tcggtgcggt actgcgctcc cgggaacgag atctgctcga cggccgcgta ggcggccgcg
     1921 cgcgccctag cggccgtggc tccggtgccg acgacgttga gcgcacggcc accggacgtg
     1981 acgtactcgc ccggacggcc cgccggggtg agcccgccgg cgaacaccat gacgtcatcc
     2041 ggcagctcgg cgggcaccgc aacgggcacg cccccgaccg ggctggtcgg gtagccgggt
     2101 gcggccatga ccacgcaggc cgaataggcg tcccgccact cggcgacggc ggaccccagc
//
LOCUS       DAOQYE010000331         2157 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_58387_length_2157_cov_3.429591, whole genome shotgun sequence.
ACCESSION   DAOQYE010000331 DAOQYE010000000
VERSION     DAOQYE010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58387_length_2157_cov_3.429591"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1033)
                     /locus_tag="PKN52_06145"
     CDS             complement(<1..1033)
                     /locus_tag="PKN52_06145"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNQ99561.1"
                     /translation="MKATVTHSRKFRMAGSAHLAVAYELRRLLAARARSLRAALLGAL
                     LGPVLAAFVGGAMAQTTITFWPSSNPEEIQFATQIVSQWNGAHPELQIKMQPLPASRS
                     TEEVLLAAIAARTTPDVAANIYPGAISQFVEAGGLYEHESLPGFMEFMTERSGEDVVA
                     LYTHPSGHVYQIPWKANPVMFAYNVKLLAEAGIEPGDLATYSGFLEAAKKVEDHWGGE
                     KHLYTPTVDVTWWQRFFDFYALYIAASQGQTLLDDNGDVVFDNDAGREVFEFLATFFR
                     EGYAPKGQTAQNRFFQGTTLVEQAGPFTMPFYENNAPEGFEFGLIPVPVPDRLAGQPV
                     YTYGDPKNIA"
     gene            complement(1045..1914)
                     /locus_tag="PKN52_06150"
     CDS             complement(1045..1914)
                     /locus_tag="PKN52_06150"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNQ99562.1"
                     /translation="MSLPTSSYALQRRLKGWPLVFRQLLFYSLLLLGALTFLMPFLYM
                     VSTSLKGVREMHTMNLIPREPTLDHFKAVFTQIPIGRAMLNSFFVYGLQTASVLVFSS
                     IVGYALARLKFFGREFIFNIILLTMMIPGQLLLIPLYILIVQFGWADSYQALIVPGMV
                     SAFGIFLFRQNFKTIPQELIDAAHVDGATELQILFRIVWPLSLPSLITVGIFTFMGGW
                     NDFLWPSIVNRELGVQTLTQMVTLYGIGGQAGGQTGAVMASSLLAVVPMVIVYLVFQR
                     YYLEGIASTGVKG"
     gene            complement(1914..>2157)
                     /locus_tag="PKN52_06155"
     CDS             complement(1914..>2157)
                     /locus_tag="PKN52_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNQ99563.1"
                     /translation="AFILVVVLSTIGGFSLFVEPFVMTAGGPLDSTLSLVLYLYQQAF
                     QFLRMGYAAAIGLVLAVLIFLVTFVQRRYLEKEVGY"
BASE COUNT          410 a          640 c          759 g          348 t
ORIGIN      
        1 tggcgatgtt cttggggtcg ccgtacgtgt acacgggctg gccggcgagg cggtctggca
       61 cagggacggg gatgagaccg aactcgaagc cctcgggcgc gttgttctca tagaacggca
      121 tggtgaaagg gccggcctgc tcgacgagcg tcgtgccctg gaagaagcgg ttctgcgccg
      181 tctgcccctt gggcgcgtag ccctcgcgga agaaggtggc gaggaactcg aacacttcgc
      241 gcccggcatc gttgtcgaag acgacgtcgc cgttatcgtc caggagcgtc tgtccttggc
      301 tagccgcgat gtacagcgcg tagaagtcga agaagcgctg ccaccacgtg acgtcgacgg
      361 tgggagtgta gaggtgcttc tcgccgcccc agtggtcctc gaccttcttg gccgcctcca
      421 ggaagccgga gtaggtggcg aggtcgcccg gctctatgcc cgcctccgcg aggagcttca
      481 cgttgtaggc gaacatgacg gggttggcct tccagggtat ctggtagaca tgccctgacg
      541 gatgcgtgta gagcgccacg acatcctcac cgctgcgctc cgtcatgaac tccatgaagc
      601 cgggcagcga ctcgtgttcg tagagcccgc cggcctcgac gaactggctg atggcaccgg
      661 ggtagatgtt ggcggccacg tccggcgtcg tgcgggcggc gatggcggcc aggagcacct
      721 cctccgtcga gcggctggct ggcagcggct gcatcttgat ctgcagctca gggtgtgcgc
      781 cgttccactg ggagacgatc tgcgtggcga actggatctc ctccgggttg gatgacggcc
      841 agaaggtgat ggtcgtctgg gccatggccc cgcccacgaa cgcggcgagg accgggccga
      901 gcaacgcccc caggagcgcg gctctgaggc tgcgcgccct ggcggctagc aagcgtcgca
      961 gttcataggc gaccgctagg tgagccgagc cggccatccg gaacttgcgc gagtgcgtca
     1021 cggttgcttt cacttcatgc ctcctcatcc tttcaccccc gtggaagcga tgccctcgag
     1081 gtagtagcgc tggaagacca ggtagacgat gaccatcggc accacggcga gcaggctgct
     1141 cgccatgacg gcgcccgtct ggccgccggc ctgaccgccg atgccgtaaa gagtgaccat
     1201 ctgcgtgagc gtctggaccc cgagctcgcg gttgacgatg gacggccaga ggaagtcgtt
     1261 ccatccgccc atgaacgtga agatgccgac cgttatcagg gacggcaggg agagcggcca
     1321 cacgatgcgg aagaggatct gcagctcggt ggcgccgtcg acgtgcgcgg cgtcgatcag
     1381 ctcctgcggg atcgtcttga agttctgccg gaagaggaag atgccgaacg cgctgaccat
     1441 gccgggcacg atgagcgcct ggtacgagtc ggcccacccg aactggacga tcaggatgta
     1501 gagcgggatg aggaggagct ggccggggat catcatcgtc aggaggatga tgttgaagat
     1561 gaactcgcgt ccgaagaact tcaggcgggc cagcgcgtag ccgacgatgg aggagaagac
     1621 gagcacgctg gccgtctgca gcccgtagac gaagaagctg ttgagcatgg cccgcccgat
     1681 ggggatctgc gtgaacacgg ccttgaagtg gtccaaggtc ggctcgcgcg ggatcaggtt
     1741 catcgtgtgc atctcacgca cgcccttgag cgacgtcgag accatgtaca ggaacggcat
     1801 caggaaggtg agcgccccga gcagcagcag ggagtagaag aggagctgcc gaaagacaag
     1861 cggccagccc ttgaggcggc gctgcaacgc gtagctgctg gtgggcaggc tcatcagtac
     1921 ccgacctcct tctccaggta gcggcgctgc acgaacgtga cgaggaagat gagcaccgcc
     1981 agcacgaggc cgatggccgc ggcgtacccc atgcgcagga actggaaggc ctgctggtag
     2041 aggtagagca cgaggctgag tgtcgagtcg agcggcccac ccgccgtcat gacgaacggc
     2101 tccacgaaca gagagaagcc gccgatggtc gatagcacga ccacgaggat gaacgcc
//
LOCUS       DAOQYE010000332         2155 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_58499_length_2155_cov_3.550000, whole genome shotgun sequence.
ACCESSION   DAOQYE010000332 DAOQYE010000000
VERSION     DAOQYE010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58499_length_2155_cov_3.550000"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..1409)
                     /locus_tag="PKN52_06160"
     CDS             complement(66..1409)
                     /locus_tag="PKN52_06160"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HNQ99564.1"
                     /translation="MPESSARLLESGPDATRPLRGVRVLAIEQYGAGPFATLVLADLG
                     AEVIKIELPEQGDVGRGIPPWTDGADSLFFQSLNRGKRSVAVDLKERRGRQLFERLVA
                     NSDAVFANTRGLSPSKLRITYADLARFNPAIVCAFLTGFGRAGPRADEPGYDYIVQAM
                     SGMAALGGEPGGPPTRAGVSVVDFATGLAAALGLLAGLHRARTTGVGGDVDTSLLLTA
                     LNLTNYVSSWVLTRGFQPERLPHGAHPSVVPSQLFEASDGWLMVMSQTDAFFRELVKR
                     MDLPEVLADARFGTMAGRLENREALLGLLEARFRERTVAQWLDRLEGAVPVAPVNDLP
                     TALRERQVVETSMLVSFQHPTFGEVRQVAGPVQVSSPPPPPTPAPALGADTWSVLHAL
                     AGLDEDTFQELLADGVVAQAPSGSGGAGENAEPTLDSDERPVVTGARTDRKRGRG"
     gene            complement(1416..>2155)
                     /locus_tag="PKN52_06165"
     CDS             complement(1416..>2155)
                     /locus_tag="PKN52_06165"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ99565.1"
                     /translation="INSAKDDAAGLQISNRLKPPSSTHPFGTDNFGRDVWARTVFGTR
                     LSLVVGLSTALITAVLGTLLALLAGTYRFLGELIMRVMDGLMALPATLLAIAIMAAVG
                     PRVENMVVALSLVYLPRSTRVARAAVLVVREQVFVEAAIALGAGNRGIMWRHILPGAL
                     SPVIVQATYVFATAVLLEASLSFLGVGAPPEVPSWGIMLSEGRLLMRNAPWMTVFPGT
                     LLALTVLSVNMLGDGLRDALDPKMKRG"
BASE COUNT          371 a          731 c          704 g          349 t
ORIGIN      
        1 gccgtcctcg agtaccttgg ccccgagctc gaccgctacc ggttggggcg caacgactat
       61 tcccttcatc ctcttcctcg cttcctatcg gttcgtgcac cggtgactac cggccgttcg
      121 tctgagtcca acgtcggttc agcgttctcg cccgctccgc cggagcccga gggtgcttga
      181 gcgacgacgc cgtcggccag cagctcttgg aacgtgtcct cgtcgaggcc agccaaggcg
      241 tgcaggacgc tccaggtatc cgccccaagc gccggcgccg gagtcggagg cggcggcggc
      301 gaggacacct gcacgggtcc cgcaacttgc ctgacctccc cgaaggtcgg gtgctggaaa
      361 gacacgagca tggaagtctc gaccacttgc cgctcacgca gggcggtcgg gaggtcgttc
      421 actggtgcca caggcactgc gccctcgagt cggtcaagcc actgggccac ggttcgctcc
      481 cggaacctcg cctcgagcag cccgagcagc gcctctcggt tctcgaggcg tccggccatc
      541 gtcccgaacc gcgcatctgc cagtacctcc gggaggtcca tccgcttgac gagttcgcgg
      601 aagaaggcgt cggtctgact catcaccatg agccacccgt cgctcgcctc gaagagctgg
      661 gagggcacga ccgacgggtg cgctccatgc ggcagtcgct ccggttggaa acctcttgtc
      721 aggacccaac tggagacgta gttcgtgagg ttcagcgccg tgaggagcag gctcgtgtcg
      781 acgtcgccgc ccacccctgt cgttctcgcc ctatgcaggc cggccagcaa ccccaaggcg
      841 gcggcaaggc ccgtggcgaa gtcgacgacg ctaacgcctg ccctcgtggg cggccccccc
      901 ggctccccac ctagcgctgc catgccgctc atcgcttgga cgatgtagtc gtagccaggc
      961 tcatccgcgc gcggtccggc tcgcccgaag cccgtgagga acgcacagac tatcgcgggg
     1021 ttgaagcggg ccaggtcggc gtaggtgatg cggagcttac ttggggaaag gccgcgcgtg
     1081 ttcgcgaaca ccgcgtctga gttggccacc aggcgctcga agagctgcct gccgcgccgc
     1141 tccttcaggt cgacggcgac gctccgcttt ccgcggttga ggctctggaa gaacaagctg
     1201 tccgccccgt ccgtccatgg ggggatgccg cgtcctacgt cgccctgctc gggtagctcg
     1261 atcttgatca cttcggcacc aagatcggcg agcacgagcg ttgcgaaagg gccggcaccg
     1321 tactgctcga tcgccaggac acgaactccg cgtagtggcc tcgttgcgtc aggcccgctc
     1381 tcgagcagcc tggcacttga ctcgggcata tgtcgtcagc ctctcttcat cttgggatcc
     1441 aaagcgtccc gcaggccgtc gccgagcatg ttgacggaca gaacggtcag ggctagaagg
     1501 gttccgggga acaccgtcat ccagggcgcg ttgcgcatga gcaggcgccc ctctgacaac
     1561 atgatccccc agctcggcac ctcaggcggc gcgccgaccc cgaggaagga cagcgaggcc
     1621 tcgagtagga ccgccgtcgc gaagacgtat gtggcctgca cgatcacggg tgagagagca
     1681 ccaggcaaga tgtggcgcca catgatcccg cggttaccgg cgcctagcgc gatggcggcc
     1741 tccacgaaga cctgctctcg caccacaagg acggcagccc gcgcgacgcg ggtggagcgg
     1801 ggcaagtaga ccaggctcaa cgccaccacc atgttctcga ccctcggtcc gacggccgcc
     1861 atgatcgcga tggccaacaa cgtcgccggc aaggccatga gcccgtccat gacacgcatg
     1921 atgagttcgc ccaggaagcg atatgtcccg gcgagcaaag ccaagagcgt gcctagaacg
     1981 gcagtgatca gggcggtact gaggccgacg accaaggaga gtcgcgtgcc gaacacggtc
     2041 ctcgcccaga cgtcacgccc gaagttgtcg gtgccgaaag ggtgggtgct agagggcggc
     2101 ttcaggcggt tgctgatctg caggccggcg gcatcgtcct tggcggagtt gatgc
//
LOCUS       DAOQYE010000333         2153 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_58600_length_2153_cov_2.963775, whole genome shotgun sequence.
ACCESSION   DAOQYE010000333 DAOQYE010000000
VERSION     DAOQYE010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58600_length_2153_cov_2.963775"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="PKN52_06170"
     CDS             <1..493
                     /locus_tag="PKN52_06170"
                     /inference="COORDINATES: protein motif:HMM:NF037182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99566.1"
                     /translation="RAEEEATRTLLRLAELSGGRRPLMVVQTHSPDHELLTALAAPDA
                     AGAIEVLVGKQLARRKRFGYPPFASLAKLQFSAPDRGSALAAAQLAADTLLTGGAGPD
                     EVLGPASAPVERLKGRYLYQLLLRAEDDVRLEELLARTPQRYPGARLAIDVDPSDVGA
                     FLD"
     gene            559..1002
                     /gene="smpB"
                     /locus_tag="PKN52_06175"
     CDS             559..1002
                     /gene="smpB"
                     /locus_tag="PKN52_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HNQ99567.1"
                     /translation="MLRNRRASHDYELLETFEAGMELTGSEVKSLRQGGGSIAEAYAR
                     VVGGEVFVENMTIPIYQEASYNNHEPTRRRRLLLNAKEIEEISKGLERRGLTLVPLKL
                     YFKDGWAKLEVALARGKKLHDKRDDAAKKDAKREIDRQLARSGRK"
     gene            999..>2153
                     /locus_tag="PKN52_06180"
     CDS             999..>2153
                     /locus_tag="PKN52_06180"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNQ99568.1"
                     /translation="MSAVGPGRLARPLIAGLLFLVCALAFAEPRLLVNGRDIAANTTE
                     LVRGTSYAPGAALARALGAELTNDLGRGIASLQVGGRVMQLSLVGEPPTGDAATPAVW
                     LDGRAVAGGPAVMTNGEVFLPVKAVAEALGASVTYLADDDTVMVVQPRARLTAIRVQE
                     RGGERVELSLSALVRYSTFYNEPLATLHVYLERTDVETRLPAVEGQAFSSATAAAAAG
                     ATEVRIQLEPGVAYRVYAVPDGRGFRLNVALGVAAGEVESDRVRVVLDPGHGGSDQGI
                     VTPGFGSEATLTLAFAERLAAALEGRGMRVTLTRTLDAPVEQAERERLGVGADLFLSF
                     HVAPVELGEFRAYYLGAAANVTSLDMAVRENAAAALRDDTTDALRRELLLG"
BASE COUNT          329 a          778 c          763 g          283 t
ORIGIN      
        1 ccgggccgag gaggaggcaa cgcgcacgct cctacggctc gcggaactga gcggcggacg
       61 aaggcccctc atggtcgtgc agacgcactc gcccgaccat gagctgctta ccgccctggc
      121 cgcccctgac gccgccggcg cgatcgaggt cctggtcggc aagcagctcg cgcggcgcaa
      181 gcgtttcggc tacccaccct tcgcgagcct cgccaagctg cagttctcgg ctcccgaccg
      241 cggcagcgct ctcgccgcgg cgcagctcgc ggccgatacc cttctcaccg gcggcgccgg
      301 acccgacgag gtgctcggcc cggcgagcgc gccggtggag cgcctcaagg ggcgctacct
      361 ctaccaactc ctcctgcgcg ccgaggacga cgtcaggctc gaggagctgc tggcccggac
      421 gccgcagcgg tacccgggcg cgaggctggc catcgacgtc gacccgagcg atgtcggcgc
      481 tttcctggat tgaggagcga cgccgggcgc caagctctca cttccgacgg ataagatgtc
      541 gcccggtagg agaccggcat gcttcgcaac aggcgcgctt cccacgacta cgaactcctg
      601 gagaccttcg aggccggcat ggaactgacc ggcagcgagg tgaagtcgct tcggcagggg
      661 ggcggctcca tcgccgaggc ctacgcgcgc gtcgtcggcg gcgaggtctt cgtcgagaac
      721 atgaccatcc cgatctacca ggaagccagc tacaacaacc acgagccgac caggcgcagg
      781 cgcctgctgc tcaacgccaa ggagatcgag gagatctcca aggggctcga gcgcaggggg
      841 ctcacgctcg tcccgctcaa gctctacttc aaggacggct gggcgaagct cgaggtagcc
      901 ctggcgcgcg gcaagaagct gcacgacaaa cgcgacgacg cggcgaagaa ggacgccaag
      961 cgcgagatag acaggcaact cgccaggtcg ggtcggaagt gagcgctgtc gggccaggtc
     1021 gcctggcgcg ccccctgatc gccggcctgc tcttcctggt ctgcgcgctc gcgttcgccg
     1081 agccgcgcct gctcgtcaac ggccgcgaca tagccgcgaa caccaccgag ctggtgaggg
     1141 gcacgagcta cgcgccaggc gccgccctcg cgcgcgcgct cggcgccgag cttacgaacg
     1201 acttggggcg cggcatcgcc tcgcttcagg tgggcggccg cgtcatgcag ctctcgctgg
     1261 tgggcgagcc gccgaccggc gacgccgcga cgccggccgt gtggctcgac ggccgcgccg
     1321 tggccggagg accggccgtc atgacgaacg gcgaggtgtt cctgcccgtg aaggccgtgg
     1381 cggaggcgtt gggagccagc gtcacctacc tggccgacga cgacaccgtc atggtcgtcc
     1441 agccgagggc gcgcctcacg gccatccggg ttcaggagcg cggcggtgag cgcgtggaac
     1501 tgagcctcag cgccctcgtg cgctactcca ccttctacaa cgaaccgctg gccaccctgc
     1561 acgtgtacct ggagcggacc gacgtcgaga cgcgcctgcc ggcggtagag ggccaggcct
     1621 tctcgagcgc gacggccgcc gcggcggcgg gggcgaccga ggtgaggatc cagctggaac
     1681 ccggcgtggc ctatcgcgtg tacgcggtgc cggacggccg cggtttcagg ctcaacgtgg
     1741 cgctgggggt cgccgccggg gaggtcgaat ccgaccgggt ccgcgtcgtg ctcgacccgg
     1801 gccacggggg ctcggaccag gggatcgtca cgcccgggtt cggcagcgag gccacgctga
     1861 ccctcgcctt cgccgagcgt ctcgccgccg cgctcgaggg ccgaggcatg cgcgtcacgc
     1921 tcacgcgcac cctcgacgct cccgtggaac aggccgagcg cgagcggctc ggggtcggcg
     1981 ccgacctctt cctctccttc cacgtggcgc ccgtggaact cggcgagttt cgcgcctact
     2041 accttggcgc ggccgccaac gtgaccagcc tcgacatggc cgtgagggag aacgcggccg
     2101 ccgcgctgcg cgacgacacc acagacgcgc tcaggcgcga gctcctgctc ggc
//
LOCUS       DAOQYE010000334         2152 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_58662_length_2152_cov_1.987601, whole genome shotgun sequence.
ACCESSION   DAOQYE010000334 DAOQYE010000000
VERSION     DAOQYE010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58662_length_2152_cov_1.987601"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..590
                     /locus_tag="PKN52_06185"
     CDS             <1..590
                     /locus_tag="PKN52_06185"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99569.1"
                     /translation="RDGRWVRPDEIRIGGGIRRGRTTQNTSQFIMTMSGHAATLWVGR
                     TIPDPSWLNHVRFRPTVVVMPRGGGAVVIPGNDPEWVWSDVGARLMVLPYYLDNGLID
                     VEIYPEVTYLDGQGRRQAIRTESVSTRLTVRDGQRVSIGGVVDENREFYVNLFGPDLL
                     SRDNSTSILDMYLTCHVMIPRDPASGGGRSSLPGR"
     gene            complement(611..1591)
                     /locus_tag="PKN52_06190"
     CDS             complement(611..1591)
                     /locus_tag="PKN52_06190"
                     /inference="COORDINATES: protein motif:HMM:NF013543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HNQ99570.1"
                     /translation="MFSSLQYLAGLFLGWGLGANDTANVFGTAVSSRMVSYRLAVVLT
                     AVFVLVGALWQGEAGIRTLSEGLSRQHAGEPAGQARQRAVLVSLGAALTVLAMTRMRV
                     PVSTSQAVVGAIIGVGLMERNVDFGGLGKVVACWLGTPVGAALLTLVFHRLLSWPVRR
                     WKPSVFVYDPVVTILLILTGAYGAYALGANNVANVSAMFVGEGMLTVRQAAIFGGLSI
                     VAGTVTYSRPVMMTVGRGIVRLDAFTALVTVLAHAVTVHIYAMVGVPVSTSQAIVGAV
                     LGIGLIKGMQTVNLRMLGRVAAGWTATPFIAAAAAAALFFLTHLRYAPDA"
     gene            complement(1647..>2152)
                     /locus_tag="PKN52_06195"
     CDS             complement(1647..>2152)
                     /locus_tag="PKN52_06195"
                     /inference="COORDINATES: protein motif:HMM:NF013982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HNQ99571.1"
                     /translation="CRRCHQEESNADDVRRQIELDMFTKSILPESREDLLNILDLVDR
                     VPGKAESVLYMFMNQRTELLADIRKDMKELVHLSVETFRYTVDAARDCFGDMARIRDF
                     SRLIDNNESLGDRLERKMIARIFAEKIPAGEKLLQKEFVVATGEICDLCEQAKDRLVI
                     WSVKRQV"
BASE COUNT          339 a          697 c          812 g          304 t
ORIGIN      
        1 tccgcgacgg caggtgggtg cggccggacg agatccgcat cggcggcggc atccggcgcg
       61 ggcggaccac gcagaacacc tcgcagttca tcatgacgat gagcggccac gccgcgacgc
      121 tctgggtggg caggacgatc cccgacccga gctggctgaa ccatgtccgc ttccggccca
      181 cggtggtggt catgccgcgc ggcggcgggg cggtggtcat ccccggcaac gacccggaat
      241 gggtgtggag cgatgtcggc gcgcggctga tggtcctgcc atactacctc gacaacggcc
      301 tgatcgacgt ggagatctac cccgaggtga cctacctcga cgggcagggg cggcgccaag
      361 cgatccgcac cgagagcgtg tcgacgcggc tgaccgtgcg cgacgggcag cgcgtctcga
      421 tcggcggggt ggtcgacgag aaccgcgagt tctacgtcaa cctcttcggc ccggacctgc
      481 tcagccgcga caacagcacg tcgatcctcg acatgtacct gacctgccac gtgatgatcc
      541 cgcgcgaccc ggcctcaggg ggcgggcgct cctcgcttcc cgggcgctga ccgcctgcct
      601 gccgccaact tcaggcgtcc ggggcgtagc gcaggtgggt gaggaagaac agggcggcgg
      661 cggccgccgc cgcgatgaac ggggtggccg tccagccggc cgccacgcgc ccgagcatgc
      721 gcaggttgac ggtctgcatg cccttgatca ggccgatgcc gaggacggcg ccgacgatgg
      781 cctgcgaggt ggagaccggg acgccgacca tggcgtagat gtgcacggtg acggcgtggg
      841 cgaggacggt gaccagggcg gtgaaggcgt cgaggcggac gatgccgcgg ccgacggtca
      901 tcatcaccgg gcggctgtag gtcacggtgc cggcgacgat gctgaggccg ccgaagatgg
      961 ccgcctggcg cacggtgagc atgccctcgc cgacgaacat ggccgagacg ttcgccacgt
     1021 tgttggcgcc gagggcgtag gccccgtagg cgccggtcag gatgaggagg atggtgacga
     1081 cggggtcgta gacgaagacg gagggcttcc agcgccgcac cggccagctc aggaggcgat
     1141 ggaagacgag cgtcagcagg gcggcgccga cgggggtccc gagccagcag gcgacgacct
     1201 tgccgaggcc gccgaagtcg acgttgcgct ccatgaggcc gacgccgatg atggcgccga
     1261 cgacggcctg ggaggtcgac accggcacgc gcatgcgggt catggccagg acggtcaggg
     1321 cggcgccgag gctgaccagg acggcgcgct ggcgggcctg gcctgcgggc tcccccgcgt
     1381 gctggcggct caggccctcg ctgagggtgc ggatgcccgc ctcgccctgc cagagcgccc
     1441 cgacgaggac gaagaccgcc gtcaggacca ccgccagccg gtagctgacc atgcgcgagc
     1501 tgaccgccgt gccgaagacg ttggcggtgt cgttggcgcc caggccccag cccaggaaca
     1561 ggccggcgag atattgcagc gaggagaaca tgccgtggcg tcccttgtcc gtggcggccg
     1621 agacggtggc cgccgggagg ttcaggctag acctggcgct tgacgctcca gatcacgagc
     1681 cggtccttgg cctgctcgca gaggtcgcag atctcgccgg tcgcgacgac gaactccttc
     1741 tgcaggagct tctcgccggc ggggatcttc tccgcgaaga tgcgggcgat catcttgcgc
     1801 tcgaggcggt cgccgaggct ctcgttgttg tcgatcaggc gcgagaagtc gcggatccgc
     1861 gccatgtccc cgaagcagtc gcgggccgcg tccaccgtgt agcggaaggt ctcgacggag
     1921 aggtggacca gctccttcat gtccttgcgg atgtccgcga gcagctcggt gcgctggttc
     1981 atgaacatgt agaggacgga ctcggccttg ccggggacgc ggtcgacgag gtcgaggatg
     2041 ttgagcaggt cctcgcgcga ctcgggcagg atcgacttcg tgaacatgtc cagctcgatc
     2101 tggcggcgca cgtcgtcggc gttcgactcc tcctggtggc agcgccggca ga
//
LOCUS       DAOQYE010000335         2151 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_58681_length_2151_cov_3.136450, whole genome shotgun sequence.
ACCESSION   DAOQYE010000335 DAOQYE010000000
VERSION     DAOQYE010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58681_length_2151_cov_3.136450"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            7..675
                     /locus_tag="PKN52_06200"
     CDS             7..675
                     /locus_tag="PKN52_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNQ99572.1"
                     /translation="MSTKRILIVEDDVDILRLLSRELEDAGFEVLAFDSGMRGLSAVR
                     ESSPDLVILDLGLPDISGQEIARRLRHTGNIPIIILTAADEVGTKVEMLNAGADDYLA
                     KPFHVEELLARVNVQLRKRTLGVTQKIGDLTIDPARRQVFWSAEEVRFSPREYELLHY
                     LAGQPGRVYSRKEIEHNVWGEELPPSSNVVDVHIANIRSKLRDAGGFGVIRTVRGVGY
                     ALKS"
     gene            699..2096
                     /locus_tag="PKN52_06205"
     CDS             699..2096
                     /locus_tag="PKN52_06205"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNQ99573.1"
                     /translation="MPRPTHPRPTHPRAGAPRLTALRLTTPRLALTWRVALLAGAAIA
                     LLAGLTMVVAYQVVRAGLYQELRRTIKEDARVVAALYSSGEGSAHTSLSGPTGGVVLQ
                     VYDPAGNLLVASRPEFERPSAALPPTDVMAGRGVPTTWSGLVDGKKMEGALAPFELGV
                     VVVLTDPAYIGAVLADLSRNLLIAAVLLVVVSLLVGYVVAAASLRPLSRLARLAGTLG
                     PDHLEPIQYDGPRDEVGRLTAALNGLVERLRQALDSQRVFLAETSHELRTPLTSLRGF
                     LDRAVRRAKPEVRDDLTDAQRVAGSMTRLVEDLLQLSRGQLVVDPNPHLVELRSDVLG
                     PVADEFPGVRVASGQPALVLGDPQRLAQMVRNLTANAVRAASGTGAVTLGLESDGGFA
                     TVSVSDDGPGIPAEMLEKIFEKFYKGAGGGSGLGLAIARQVAEHHSGAIEVESWPGST
                     VFRVRLPLADAGEDE"
BASE COUNT          310 a          769 c          739 g          333 t
ORIGIN      
        1 tctagcatgt ctacaaaacg catcctgatc gtcgaagacg acgtggacat cctgcggctg
       61 ctcagccgcg agctggagga cgccggtttc gaggtcttgg cgttcgacag cggcatgcgt
      121 ggcttatccg cggtccgcga gtccagtcct gacctggtca tcctagacct cggcctccct
      181 gacatcagcg gccaggagat cgccagacgc ctgcgtcata ccggcaacat acctatcatc
      241 atcctcacgg ccgccgacga ggtcggcacc aaggtggaga tgctcaacgc cggcgccgac
      301 gactacctgg ccaaaccctt ccacgtcgag gagctgctcg cgcgcgtcaa cgtccagttg
      361 cgtaagcgca ctctgggcgt cacgcagaag atcggcgact tgaccatcga tccggcgcgc
      421 aggcaggtct tctggtcggc ggaggaagtg cgcttcagcc cacgcgagta cgaactcctg
      481 cactacctgg ccggccagcc cggcagggtc tacagccgca aggagatcga gcacaacgtg
      541 tggggggagg aactcccacc ctcgtcgaac gtcgtggacg tgcatatcgc caacatcagg
      601 agcaaactcc gcgatgccgg cggcttcggg gtgattcgca ccgtccgcgg cgtcggctac
      661 gccctcaagt cctgacagga tcggcggctt agctccgcgt gccgcgcccc acccacccgc
      721 gccccaccca cccgcgcgcc ggcgctccgc gcctgacggc gctgcgtttg acgacgccac
      781 gcctggccct cacttggcgc gtggcgctgc tggcgggcgc ggccatcgcg ctcctggccg
      841 ggctcacgat ggtagtcgcg taccaggtcg tgcgcgccgg cctgtatcag gaactgcggc
      901 gcacgatcaa ggaggacgcg cgggtcgtgg ccgcgctcta cagttccggc gagggcagcg
      961 cgcacacgag cctctccggt ccgacgggcg gcgtcgtgct gcaggtgtac gacccggccg
     1021 gcaacctgct cgtggccagc cgcccggagt tcgagcgccc gtcggcggcg ctcccgccta
     1081 ccgacgtcat ggccgggcga ggcgtcccga cgacctggag cggcctggtc gacgggaaga
     1141 agatggaggg cgcgttggcg cccttcgagc ttggcgtcgt cgtcgtgctg accgaccccg
     1201 cctacatcgg cgccgtgctc gccgacctgt cgcgcaacct cctgatcgcc gccgtcctct
     1261 tggtcgtcgt cagcctgctc gtcggctacg tcgtcgcggc agcctctctg cgacccctct
     1321 cccgcttggc gcgcctggca ggtacgctcg gccctgacca cctggagccc atccagtacg
     1381 acggtccgcg cgacgaggtc gggcgcctga cggccgccct caacgggctg gtcgagcggc
     1441 tgcgccaggc gctcgactcc cagcgggtgt tcctggccga gacgagtcat gagctgcgca
     1501 cgccgctcac gagcctgcgg gggttcctcg accgcgccgt caggcgcgcc aagccggagg
     1561 tgcgcgacga cctcacggac gcgcaacgcg tggccggcag catgacgcgc ctcgtcgagg
     1621 acctattgca gctctcgcgc ggtcagttgg tcgtcgaccc caacccccac ctcgtcgagc
     1681 tccgctccga cgtgctcggg ccggtggcgg acgagttccc cggggtcagg gtggcctccg
     1741 ggcagccggc gctggtgctc ggcgatccgc agcggctcgc ccagatggtc cgcaacctga
     1801 cggccaacgc cgtgcgcgcg gcgtccggca cgggggccgt gacccttggg ctcgagtcgg
     1861 acggcggttt cgccaccgtg agcgtgagcg acgacggccc cggcatccct gccgagatgc
     1921 tggagaagat cttcgagaag ttctacaagg gcgccggggg cggctcgggc ctcgggttgg
     1981 ccatcgcccg gcaggtggcg gagcaccact ccggggccat cgaggtggag agctggccgg
     2041 gctcgaccgt cttcagggtg cggttgccgc tcgcggacgc cggcgaggac gagtgacgtc
     2101 gaactaccgg gccttgggcc tctgagggat cagctgaccg ggttggcgcc t
//
LOCUS       DAOQYE010000336         2150 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_58720_length_2150_cov_3.692124, whole genome shotgun sequence.
ACCESSION   DAOQYE010000336 DAOQYE010000000
VERSION     DAOQYE010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58720_length_2150_cov_3.692124"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..754
                     /gene="selD"
                     /locus_tag="PKN52_06210"
     CDS             <1..754
                     /gene="selD"
                     /locus_tag="PKN52_06210"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HNQ99574.1"
                     /translation="LSAMTILAYPLGTLPDSVLTELMQGASEIIAEAGAVLVGGHSIE
                     DDTLKFGFSVAGVAHPERLWTNAGARPGDVAVLTKAVGTGTLSGALKSGEVEALDMAE
                     AVASMRQVNRLELPDDLHVAVHAATDVTGFGLLGHSLQVARASGVAIRLDPAGVPVLA
                     GAREALAAGSLTRAHRSNADYVAASVEGREALDEVTWLTLVDPQTSGGLLLFVAPEQV
                     DAVVAHASKRFPRTSRVGVVEELGAGRPLRLA"
     gene            complement(832..1155)
                     /locus_tag="PKN52_06215"
     CDS             complement(832..1155)
                     /locus_tag="PKN52_06215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99575.1"
                     /translation="MWIRTLDDRVINSQQIESLEVVETYADEVDPQDIEAELVEPEYF
                     EIVAVLASGDEALVHVCEDEQEAFLAYDLLTATLARGTYRDGTRVSEVASLVDLLERE
                     RQSHN"
     gene            complement(1175..1969)
                     /locus_tag="PKN52_06220"
     CDS             complement(1175..1969)
                     /locus_tag="PKN52_06220"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ99576.1"
                     /translation="MNRALFGLVLLGLIALSALFAPQLTGYDPIKPDYRARYVGPGDG
                     HVLGTDGLGRDVLARSLAGGRVSLVVALSATLLTFALGASLGVLAAGVGGATDLVLSR
                     VFDALLAFPGFLLILIVVATLGGGSLQTVMAMGVAGAPVFFRLARSFTRQELRAEYVT
                     AARALGAGRGRQLLRHAVPNFLGSVVVQMASTAAAFLLVEASLSFLGLGVPLPTPSWG
                     NVLQDARSYLTTQPWAAVGPGLFLAGASLSFQFMSDGLREILDRRS"
     gene            complement(1966..>2150)
                     /locus_tag="PKN52_06225"
     CDS             complement(1966..>2150)
                     /locus_tag="PKN52_06225"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNQ99577.1"
                     /translation="IVVEQVFGLPGLGATILGAIGARDYPVVQGVTILAVTVYVLVNW
                     LADLSQVIADPRLRAA"
BASE COUNT          342 a          714 c          806 g          288 t
ORIGIN      
        1 cttgagcgcg atgacgatac tcgcctatcc cttgggaacg ttgcccgact cggtcctcac
       61 ggagctgatg caaggcgcgt ccgagatcat cgcggaggcg ggcgccgtcc tcgtcggcgg
      121 gcactccatc gaggacgaca ccctgaagtt cggcttcagc gtggccggcg tcgcccaccc
      181 cgagaggctg tggacgaacg ccggcgccag gccgggcgac gtcgccgtac tcacgaaggc
      241 cgtcgggact ggcacgctgt ctggcgccct caagtccggc gaggtcgagg cgttggacat
      301 ggccgaggcg gtcgcctcca tgcggcaggt gaaccggctg gagctgccgg acgacctgca
      361 cgtcgccgtc cacgccgcga ccgacgtcac gggtttcggg ttgcttggcc actccctgca
      421 ggtcgcgcgc gcgtccggcg tcgccatccg gctcgaccct gctggcgtcc ccgtgctggc
      481 cggagccagg gaggctctcg cggcgggcag tctgacgcgc gcccaccgtt ccaacgccga
      541 ctacgtggcg gcttccgtcg agggtcgaga ggcgctcgac gaggtcacgt ggctcacgct
      601 ggtcgacccc cagacgagcg gcggcctcct cctcttcgtg gcgcccgaac aggtcgacgc
      661 cgtcgtcgcc cacgcctcca agaggttccc gcgcaccagc cgggtcggcg tggtggagga
      721 gctgggcgcc ggcaggccct tacgcctcgc ctgagggcgg catccggcag gtaggtctgc
      781 aggtcgggcg cctacggcgc cgcggcgggt cgtcagagaa gctttaagaa cttagttgtg
      841 cgactgccgc tcgcgctcga ggaggtcgac caacgaggcc acctcgctca ctctcgtgcc
      901 gtcgcgatac gtgcctcgcg ccagcgtcgc cgtgaggagg tcgtacgcga ggaacgcttc
      961 ctgctcgtcc tcgcagacat gcaccagcgc ctcgtcgccc gaggcgagca cggccacgat
     1021 ctcgaagtac tccggctcca cgagctcggc ctcgatgtcc tgcgggtcga cctcgtcggc
     1081 gtaggtctcg acgacttcga ggctctcgat ctgttgggag ttgatgacgc ggtcgtcgag
     1141 ggtacggatc cacatgggcc gattctacgg ttcctcagga acggcggtcg aggatctcgc
     1201 gcaggccgtc ggacatgaac tggaacgaca gggaggcgcc ggccaggaag aggccagggc
     1261 cgacggcggc ccacggctgg gtggtgaggt aggagcgtgc gtcttgcagc acgttgcccc
     1321 aactcggggt cgggaggggc acgccgagac cgaggaacga gagggacgct tccacgagca
     1381 ggaaggcggc ggcggtgctg gccatctgga cgacgacgct cccgaggaag ttgggcacgg
     1441 cgtggcgcag gagctgccga ccgcggccgg cgcccagcgc ccgggcggcg gtcacgtact
     1501 cggcacggag ttcctgacgc gtgaacgagc gcgccagccg gaagaacacg ggtgcgcccg
     1561 ccacccccat ggccatgacc gtctgaaggg agcccccacc gagggtcgcg acgacgatga
     1621 ggatgaggag gaagccgggg aaggcaagga gcgcgtcgaa cacgcgcgag aggaccaggt
     1681 cggttgcccc gccgacgccg gccgccaaca cgccgagcga ggcgccgagc gcgaaggtga
     1741 ggagggtggc cgagagcgcc accacgagcg acacgcggcc cccggccagg gagcgcgcga
     1801 gcacgtcgcg ccccaggccg tccgtgccga gcacgtgccc gtcgcccgga ccgacgtagc
     1861 gggcgcggta gtcgggcttg atggggtcgt agcccgtgag ctgcggcgcg aagagggccg
     1921 agagggcgat caggccgagg agcaccaggc cgaagagcgc ccggttcatg ctgctctcag
     1981 gcgcgggtcg gcgatgacct gcgagaggtc ggccagccag ttgacgagga cgtagacggt
     2041 cacggcgagg atggttacgc cttggacgac cgggtagtcg cgggcgccga tggcgccgag
     2101 gatggtagcg cccagccccg ggagtccgaa gacctgctcg acgacgatgg
//
LOCUS       DAOQYE010000337         2149 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_58765_length_2149_cov_3.262655, whole genome shotgun sequence.
ACCESSION   DAOQYE010000337 DAOQYE010000000
VERSION     DAOQYE010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58765_length_2149_cov_3.262655"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="PKN52_06230"
     CDS             <1..238
                     /locus_tag="PKN52_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99578.1"
                     /translation="VTDLILITLWTFNTFGPFLLTGGGPAFRTEVVSVLTYRMAFKYF
                     DFGTGSALGMVMMLINLGFALVYLGLLRRQGGSR"
     gene            235..1065
                     /locus_tag="PKN52_06235"
     CDS             235..1065
                     /locus_tag="PKN52_06235"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNQ99579.1"
                     /translation="MKVVSHVLGRMAFYVASAVFAAVFALPLVWLVFAPFGAKAKLSV
                     ALPDPFTLSNFGKVFANTFAVRALFQNSIIQAVGTMLLVTLLATLAAYALSRAPIRGR
                     DALSYGLVLFSSIVTGTAAMVPIFLLIFNMGLIDTHMGVILVFTGGLLPAAIFIMRDF
                     VDSIPRSYEESALVAGASPWGMFRDIALPIVRPGMMVVGVWTFVNVWGGFLVPFILLR
                     SPTKMPAAVAIYSFYTEGGTPIITLTTAYALLYTLPVLALYLFVNWKYGFRFFGGIKS
                     "
     gene            1079..>2149
                     /locus_tag="PKN52_06240"
     CDS             1079..>2149
                     /locus_tag="PKN52_06240"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ99580.1"
                     /translation="MANVTFLSITKQFGSFTAVNDVSFEIQDGEFVCLLGPSGCGKTT
                     TLRMIAGLEQPTSGRILIGQDDVTDSKPKERDIGMVFQDYALFPHMTIEDNIAYPLKV
                     RGVGQAERRRRAAEVAKNLSIDQLLERRPGQISGGQQQRTSVARAVVHKPRVYLFDEP
                     LSNLDAKLRLEARSFLKHLQRELGVTSIYVTHDQAEAMAMADRVAVMDRGQIVQFGPP
                     LTVYREPATTFVANFLGSPPMNLLPVRYGGGVFKAATFELDAGPFADRLRGVAAGSQL
                     TLGVRPEHVRMGGDGDRWRIPGEVYAVEPLGPESLVTLRVAGDLVTARLFGDDPPAAA
                     GATSFAFEPVNLHVFGPDGRRLA"
BASE COUNT          309 a          789 c          663 g          388 t
ORIGIN      
        1 tgtcacggat ctcatcctca tcaccctctg gacgttcaac acgttcgggc cgttcctgct
       61 caccgggggc gggccggcgt tccgcacgga ggtcgtctcc gtactcacct accggatggc
      121 gttcaagtac ttcgacttcg gaacgggcag cgccctcggc atggtgatga tgctcatcaa
      181 cctcggcttc gcgctcgtgt acctcggcct cttacggcgg cagggagggt cgcgatgaag
      241 gtcgtctcac acgtgctcgg gcgcatggcc ttctacgtgg cctcggcggt cttcgcggcg
      301 gtcttcgccc tgccgctggt gtggctcgtc ttcgcgccgt tcggcgccaa ggccaagctc
      361 tcagttgcgc ttcccgaccc gttcactctc tcgaacttcg gcaaggtctt cgcgaacacg
      421 ttcgcggtgc gggcgctctt ccagaacagc atcatccagg ccgtggggac catgctcctc
      481 gtgacgctcc tcgcgaccct cgccgcctac gcgctctcgc gcgcgcccat ccggggccgc
      541 gacgccctca gctacggcct ggtcctcttc tcgtccatcg tgaccggcac cgccgccatg
      601 gtgcccatct tcctgctcat cttcaacatg gggctgatcg acacgcacat gggcgtgata
      661 ctcgtgttca ccggcggcct gctgccggcg gccatcttca tcatgcgcga cttcgttgac
      721 tccatccccc gctcgtacga ggagtcggcc ctcgtggcgg gcgccagtcc gtggggcatg
      781 ttccgcgata tcgccctgcc catcgtgcgg ccagggatga tggtcgtcgg ggtgtggacg
      841 ttcgtgaacg tgtggggcgg cttcctagta cccttcatcc tgctgcgcag cccgaccaag
      901 atgccggccg ccgtcgccat ctactccttc tacacggagg gcggcacgcc catcatcacg
      961 ctcaccacgg cctacgcgct gctctacacg ctgccggtgc tggccctcta cctgttcgtc
     1021 aactggaagt acggtttccg cttcttcggc gggatcaagt cgtgaggggt tgaggacctt
     1081 ggccaacgtc actttcctat cgatcaccaa gcagttcggc agcttcacgg ccgtcaacga
     1141 cgtctcgttc gagatccagg acggcgagtt cgtgtgcctc ctcggcccct ccggatgcgg
     1201 caagacgacc acgctgagga tgatcgccgg actcgagcag cccacgagcg gccgcatcct
     1261 cataggccag gacgacgtga ccgactccaa gccgaaggag cgggacatcg gcatggtctt
     1321 ccaggactac gccctcttcc cgcacatgac catcgaggac aacatcgcct acccgctgaa
     1381 ggtgcgcggg gtcggccagg cggagcggcg caggcgagcg gccgaggtcg cgaagaacct
     1441 ctccatcgac cagctcctcg agcggcgccc cggtcagatc tcgggcggcc aacagcagcg
     1501 cacgtcggtc gctcgcgccg tggtgcacaa gccacgggtg tacctcttcg acgagcccct
     1561 ctccaacctc gacgccaagt tgcgcctcga ggcgcgcagc ttcctcaagc acctgcaacg
     1621 cgagctcggc gtcacctcca tctacgtcac ccacgaccag gcggaggcca tggccatggc
     1681 cgaccgcgtc gccgtgatgg accgcggcca gatcgtccag ttcggcccgc cgctcacggt
     1741 ctaccgggag cccgccacga ccttcgtggc caacttcctc gggagcccgc ccatgaacct
     1801 cctgcccgtc aggtacgggg gcggcgtgtt caaggcggcc acgttcgaac tcgacgccgg
     1861 cccgttcgcc gaccggctcc ggggcgtcgc ggcggggagc cagctcacgc tgggcgtccg
     1921 ccccgagcac gtgcgcatgg gcggcgatgg cgaccgctgg cggatccccg gcgaggtcta
     1981 cgccgtcgag cctctcggcc ccgagtcgct cgtcaccctg agggtcgccg gcgacctcgt
     2041 gaccgcccgg ctgttcggcg acgacccgcc cgcggcggcc ggcgcgacca gcttcgcctt
     2101 cgaaccggtc aacttgcacg tgttcggtcc agacggtcgg cgcctggcc
//
LOCUS       DAOQYE010000338         2148 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_58808_length_2148_cov_2.960822, whole genome shotgun sequence.
ACCESSION   DAOQYE010000338 DAOQYE010000000
VERSION     DAOQYE010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58808_length_2148_cov_2.960822"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="PKN52_06245"
     CDS             complement(<1..345)
                     /locus_tag="PKN52_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99581.1"
                     /translation="MPGGRPTLAKAFLTTLVGIALGVPTIWFVLYLVFADRWWWLFVA
                     NSLAPVLFIPVPFAMLVAWLLRRKRLVVAGLFPVAIAAYLWAGTYFPARADGPAVVDP
                     RETVSVMTFNVKA"
     gene            455..1228
                     /locus_tag="PKN52_06250"
     CDS             455..1228
                     /locus_tag="PKN52_06250"
                     /inference="COORDINATES: protein motif:HMM:NF012350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99582.1"
                     /translation="MQEPAHPKPASGHASALDGSTTSGRTSSELLLDELAAERGRNRL
                     AAVLTGLTGLGLAVGVLAVVIGRAYGAPSTGWLADYLGVGGTPGLIAAVGLAVAFLAG
                     GVPAAAEAIAELTREGKLDIDLLMVLAAVAAALVGEPRDGGVLLFLFSLAGTLEDNAF
                     TNTKGAVAALMQLKPETATLLDDGEARVVPADSVPIGARLLVRPGERVPLDGVLESGA
                     SSVDQSPITGESVPVDKEVGDALFAGSIGRTDLGGGSRT"
     gene            1230..>2148
                     /locus_tag="PKN52_06255"
     CDS             1230..>2148
                     /locus_tag="PKN52_06255"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HNQ99583.1"
                     /translation="MTKDASSSTLARMIELVTVARASRSPSQRFSDWFGQRYTVLVIV
                     GTALALGAFLLLGTEPRAAFYKAATLLVVASPCAIVISVPAAVLSALARAARMGVLFK
                     GGGALERFGAVDTVAFDKTGTLTEGRLHLTDALAFGASEDDALAAAAAIEAASDHPLA
                     KTLCRAATARGLVGPAPSDVVAVPGKGLVGKLAGLPHWAGNRKLAAELGIQLPDEVSQ
                     ALSRLEGQGKTVVLFGDPARVTGLFGLADVVRPSAAGALSRLADAGVKRFAMLTGDHA
                     TVAAGVARTLGLAADSVRSDLLPDEKVAAV"
BASE COUNT          307 a          768 c          780 g          293 t
ORIGIN      
        1 ggccttcacg ttgaacgtca tcaccgacac cgtctcccgc gggtcgacca cggctgggcc
       61 gtcggctcgc gctgggaagt acgtgccagc ccacaggtac gcggcgatgg ccacggggaa
      121 gaggcccgcg acgaccagcc gcttccgccg caacaaccaa gccaccagca tggcgaacgg
      181 caccggtatg aacaggacgg gtgccaagga gttggcgacg aacagccacc accagcggtc
      241 ggcgaacacc aggtaaagga cgaaccagat ggtcgggacg ccgagcgcta taccgacgag
      301 cgtcgtcagg aaggccttcg ctagagtggg tcggccgcct ggcatcgtcg cagcgtaacg
      361 cggtggagga tgagggaaga cctggcttgg agggtgggca gcggccccag gcgaacatgt
      421 tgtaatcgga acgattccgg gttagcctgt cgccatgcaa gagcccgcgc acccgaagcc
      481 ggcaagcggt cacgcaagcg ccctcgacgg aagcaccacc tccggccgga cctcatcgga
      541 gctgctcctg gacgagctgg cggccgagcg cggccgcaac cgcctggccg ccgtcctgac
      601 gggcctcacc gggctcgggt tggcggtagg cgttctcgcg gtggtgatcg gcagggcgta
      661 cggcgccccg tccaccggtt ggctcgccga ctacctcggc gtcggcggca cgccggggct
      721 gatcgcagcg gtgggtctcg ccgtcgcgtt cctcgccggg ggcgtgccgg ccgcggccga
      781 ggccatcgcg gagttgacgc gcgagggcaa gctcgacatc gacctgctga tggtgctcgc
      841 ggccgtggcg gccgcgctcg tcggcgaacc ccgcgacggc ggcgtgctgc tcttcttgtt
      901 cagcctcgcg ggaacgctcg aggacaacgc gttcacgaac acgaaggggg ccgtggcggc
      961 gctgatgcag ttgaagccag agacggccac gctgctcgac gacggcgagg cgcgcgtcgt
     1021 gcccgccgac tccgtgccca tcggagcgcg cctgctcgtg cgcccgggtg agcgcgtgcc
     1081 cctcgacggc gtgctggaga gcggcgcgtc gagcgtcgat cagtcgccca tcaccgggga
     1141 gtcggtgccc gtcgacaagg aggtcggcga cgccctcttc gccggcagca tcggccgcac
     1201 cgacctcggc ggcggctctc gaacttaggg tgacgaagga cgcctccagc tccacgctgg
     1261 cgcgcatgat cgagctcgtc accgtcgcaa gggcgagccg ctcgcccagc cagcgcttca
     1321 gcgactggtt cggccagcgc tacacggtcc tcgtcatcgt cggaacggcg ctcgccctcg
     1381 gagcgttcct cctcctaggg acggagccgc gcgccgcctt ctacaaggcg gccaccctcc
     1441 tcgtggtggc gagcccgtgc gccatcgtga tcagcgttcc ggccgcggtc ctctcggcgt
     1501 tggcgcgcgc agcccgcatg ggcgtgctct tcaagggcgg cggggccctc gagcgcttcg
     1561 gcgccgtgga cacggtcgcg ttcgacaaga cgggcaccct cacggagggt cgcctacacc
     1621 tcaccgacgc gctggcgttc ggcgcgagcg aagacgacgc cctggccgcc gccgcggcca
     1681 tagaggccgc gtcggaccac cccctcgcca agacgctctg ccgggcggcc accgcccgcg
     1741 gcctcgtcgg tccggcgccg tcggacgtgg tggccgtgcc cggcaagggc ctcgtcggca
     1801 agctggccgg gctgccgcac tgggcgggca accgcaagct ggcggccgag ctcggcatcc
     1861 agttgccgga cgaggtctcg caagcgctgt cgcggctcga gggccaaggg aagaccgtgg
     1921 tcctattcgg cgaccccgcg cgggtcaccg gcctcttcgg cctggcggac gtcgtgcgcc
     1981 cgagcgcggc cggcgccttg tcgcgcctcg ccgatgccgg cgtgaagcgc ttcgcgatgc
     2041 tcaccggcga ccatgcgacc gtcgccgccg gggtggcgcg cacgctcggc ctggcggccg
     2101 actcggtgcg ctcggacctc cttccggacg agaaggtcgc ggcggtag
//
LOCUS       DAOQYE010000339         2147 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_58855_length_2147_cov_3.223231, whole genome shotgun sequence.
ACCESSION   DAOQYE010000339 DAOQYE010000000
VERSION     DAOQYE010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58855_length_2147_cov_3.223231"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..>2147)
                     /locus_tag="PKN52_06260"
     CDS             complement(5..>2147)
                     /locus_tag="PKN52_06260"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0032549 - ribonucleoside binding [Evidence
                     IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HNQ99584.1"
                     /translation="QFKDQVNPLSELRHKRRISALGPGGLTRERAGFDVRDVHRTHYG
                     RICPIETPEGPNIGLITSLASFAKVNDRGFIEAPYRRVVNARVTDEVVYINAHDEDKH
                     RIAQANTPLNDDGTFATETIVARDLGDAVFLTPDEVDFMDVSPKQIISVPTSLIPFLE
                     HDDANRALMGSNMQSQAVPLIRAQAPFVGTGVEERVSRDSGTVVLATEPGTVTYVDAN
                     RIVVENDKGIEREYQLTRFQRSNQNTNLNQRPVVDIGDRVVPNQTIADGPASEVGRLA
                     LGQNVLIALMPFDGYNFEDAIVLSENLVRSDAFTSVHIEKFEKEARDTKLGPEKITRD
                     IPGLSEAALRDLDEDGVVRIGAEVRQGDILVGHTSFKGEKDPTPEERLLRSIFGEKAK
                     EVKDTSLKVPPGDGGIVLRTVRFRKGDAGVELKPGVQEMVRVYIAQKRRLIEGDKLAN
                     RHGNKGVVAKILAPEDMPFLADGTPVDMVFNPLGVPSRMNLGQILETHLGLAALKHGV
                     SYVTPVFDGAREGEIKDLLEASAKAEAAADAAAGFEPDARALEVYRRAGKLGIIDRDL
                     TTTEAHVALARSGKTVLFDGRTGEAIDAPIVVGIMYIMKLYHMVEDKMHARSTGPYSL
                     ITQQPLGGKAQFGGQRLGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDG
                     NHQMDAGMPESFNVLVKEIRSLGINMELEQD"
BASE COUNT          297 a          692 c          752 g          406 t
ORIGIN      
        1 ttcattagtc ctgctccagc tccatattga tgcccagcga ccggatttcc ttcaccagca
       61 cgttgaagga ctccggcatg cccgcatcca tctggtgatt gccgtcgacg atgttcttgt
      121 acatcttcgt gcggccgttc acgtcgtccg acttgaccgt cagcatctcc tgcagcgtgt
      181 acgccgcgcc gtaggcctcg agcgcccaga cctccatctc gcccaggcgc tggcccccga
      241 actgggcctt gcctcccagc ggttgctgcg tgatgagcga gtaggggccg gtggagcgcg
      301 cgtgcatctt gtcctcgacc atgtggtaga gcttcatgat gtacatgatc ccgaccacga
      361 tgggcgcgtc gatggcctcg cccgtgcgac cgtcgaagag cacggtcttg cccgagcggg
      421 cgagcgcgac gtgcgcctcg gtggtggtca ggtcgcggtc gatgatgccg agcttgccgg
      481 cgcggcggta gacctcgagg gcgcgagcgt ccggctcgaa gccggccgcg gcgtcggctg
      541 ccgcctcggc cttcgcggac gcctcgagga ggtccttgat ctcgccctcg cgcgcgccgt
      601 cgaacacggg cgtcacgtag gagacgccgt gcttgagggc cgcgaggccc aggtgggtct
      661 cgaggatctg accgaggttc atgcgcgacg gaacgccgag cgggttgaag accatgtcga
      721 ccggggtgcc gtcggctagg aacggcatgt cttccggcgc caggatcttg gcgacgacgc
      781 ccttgttccc gtggcggttg gccagcttgt cgccctcgat caggcgacgc ttctgcgcga
      841 tgtagacgcg gaccatctcc tgcacgcccg gcttgagctc gacgcccgcg tcgcccttgc
      901 ggaaacgcac cgtgcgcagc acgatgccgc cgtcgcccgg cggcaccttg agcgacgtgt
      961 cctttacttc cttggccttc tcaccgaaga tcgagcgcag gagccgctcc tctggcgtcg
     1021 ggtccttctc ccccttgaag ctcgtgtgac cgacgaggat gtcgccttgg cgcacctctg
     1081 cgccgatgcg caccacaccg tcctcgtcga ggtcgcgcag cgcggcctcg gagaggccgg
     1141 ggatgtcgcg ggtgatcttc tccggcccga gcttcgtgtc gcgcgcttcc ttctcgaact
     1201 tctcgatgtg cacgctcgtg aacgcgtcgg agcgcacgag gttctccgag agcacgatgg
     1261 cgtcctcgaa gttgtagccg tcgaacggca tgagcgcgat gaggacgttc tgaccgagcg
     1321 ccagacggcc gacctcggag gcaggcccgt cggcgatggt ctggttgggg acgacgcggt
     1381 cgccgatgtc gacgacgggc cgttggttca ggttcgtgtt ctggttggag cgctggaagc
     1441 gcgtgagctg gtactcgcgc tcgatgccct tgtcgttctc gaccacgatg cggttggcgt
     1501 cgacgtaggt gaccgtgccc ggctcggtcg cgagcacgac ggtgccggag tcgcgcgaca
     1561 cgcgctcctc gacgcccgtg ccgacgaacg gcgcctgggc gcggatgagg ggcaccgcct
     1621 gcgactgcat gttggatccc atgagcgcgc ggttggcatc gtcgtgctcg aggaacggga
     1681 tgagcgaggt cggcaccgag atgatctgct tcggcgagac gtccatgaag tcgacttcgt
     1741 ctggcgtcag gaacacggcg tcgccgagat cgcgggcgac gatcgtctcc gtcgcgaacg
     1801 tgccgtcgtc gttgaggggc gtgttcgcct gagcgatgcg gtgcttgtcc tcgtcgtggg
     1861 cgttgatgta cacgacctcg tccgtgacgc gggcgttcac gacgcggcgg tacggcgcct
     1921 cgatgaagcc gcgatcgttc accttcgcga agctcgccag cgacgtgatg aggccgatgt
     1981 tcgggccttc cggggtctcg atcgggcaga tgcggccgta gtgcgtacgg tgcacgtcgc
     2041 gcacgtcgaa gccggcgcgc tcgcgcgtga gaccgccggg gccgagggcg gagatccggc
     2101 gcttgtggcg cagctcggac agcgggttga cctggtcctt gaactgg
//
LOCUS       DAOQYE010000340         2147 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_58862_length_2147_cov_2.902008, whole genome shotgun sequence.
ACCESSION   DAOQYE010000340 DAOQYE010000000
VERSION     DAOQYE010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58862_length_2147_cov_2.902008"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1384)
                     /locus_tag="PKN52_06265"
     CDS             complement(<1..1384)
                     /locus_tag="PKN52_06265"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HNQ99585.1"
                     /translation="MPASEWVYRGAAEQFQGFLTGELDPVELTEAYLARIGAVDGQLR
                     SFITVLGDKAAEAARRSAERFQAGAPLSRLDGIPFAVKDQMLIEGVRVTGGSRVLADF
                     VGGRDATVVGRLREAGAVLLGTLNTHEFHSGMTRDPLFGKARNPWNLERSPGASSSGS
                     AAAVAAGLCSFSLGGDTGGSIRAPASYCGVVGVKATWSRVSRDGVIPLAYSMDCVGPL
                     ARRVEDTAMVLEHLAGADPLDPTASHLPVPDYAAGLTCDLAGLRIGVITEMLDPAVVQ
                     PAAIAATEAAVTVLRELGASVVDVSIPLLHQTRFVSVALIMAEAASHHRKWLLERYED
                     YDVNTRTNFLTGALLPAGALGQAQRMRSLVARQVVGCLEEVDVLVGPTADAAFPLQPR
                     DPLPLREEVRASLRMPAMASGPQTRVFSLSGHPSLSVPCGFDPDGLPLGLQLATRHFD
                     EATLLRVAHAY"
     gene            complement(1371..1592)
                     /locus_tag="PKN52_06270"
     CDS             complement(1371..1592)
                     /locus_tag="PKN52_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99586.1"
                     /translation="MSGKTEPNPAEFSTADVATMLAAQGYRVPDSDLEEITERLRGLV
                     AGLMAYDAYRPFDVEPWAEWPRVVADAGE"
     gene            complement(1643..>2147)
                     /locus_tag="PKN52_06275"
     CDS             complement(1643..>2147)
                     /locus_tag="PKN52_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99587.1"
                     /translation="PLVYQAKMMSVFPGPRAAQAYTGEILKIPSCGSFHGGAGFDSDL
                     EDSWMDANYRGIRNVMRHLGMEEGAIERPKRYLVYETVQRINPRNGGYLKPERRRDTF
                     GREVQAGELLGKVISPFTFETIEELRAPVGGYLGYWARNYPVHPGDWAFAVVPRDHAG
                     TRWIEAG"
BASE COUNT          343 a          745 c          742 g          317 t
ORIGIN      
        1 cgtaagcgtg cgcgacccgc agcagcgtgg cctcgtcgaa gtgcctggtg gccaactgca
       61 ggcctagcgg aagcccgtcc gggtcgaagc cgcacgggac ggacagcgac ggatggcccg
      121 agaggctgaa caccctggtc tgcggtccgc tcgccatggc cggcatcctc agcgaagcgc
      181 gcacctcctc gcgcagcggc agggggtcac gcggctgcaa cgggaacgct gcgtcggcgg
      241 tcggcccgac gagcacgtcc acctcttcca gacaacccac gacctgccgg gccacgaggg
      301 agcgcatgcg ctgagcctgg cccaaggcac cggcaggcag tagcgcgccc gtcaggaagt
      361 tggtgcgggt gttcacgtcg tagtcctcgt agcgctccag caaccacttg cggtggtggc
      421 tggcggcctc ggccatgatg agggcgacgg agacgaagcg cgtctggtgc agcaacggta
      481 tggatacgtc gaccacggag gcgcccagct ctcgcagcac cgtcaccgcc gcttccgtcg
      541 ccgcgatagc ggcgggttgg acgacggcgg gatcgagcat ctctgtgatg actccgatgc
      601 gcaggcctgc caggtcgcac gtcaggcccg cagcgtaatc gggaacaggg agatggctgg
      661 cggtggggtc gagggggtcg gcgccggcca ggtgctcgag caccatggca gtgtcctcca
      721 cccggcgggc gagagggccg acgcagtcca tcgagtaggc caacggtatg acgccatcgc
      781 ggctgacgcg cgaccaggtc gccttgacgc cgaccacgcc gcagtaggac gccggggcgc
      841 ggatggagcc acccgtgtct ccgcctagcg agaaggagca caaaccggcg gcgacggccg
      901 ccgccgaccc gctggaagac gcgcccggcg agcgctcgag gttccacggg ttcctggcct
      961 tcccgaagag cgggtcgcgg gtcatcccgg agtggaactc gtgggtgttg agggtaccga
     1021 gcaggacggc gcccgcctcg cgcaggcgcc cgacgacggt ggcgtctcgc cctcccacga
     1081 agtcggccaa cacgcgtgac cccccggtca ctcgcacacc ctcgatgagc atctgatcct
     1141 tcaccgcgaa ggggatgccg tcgaggcgcg acaagggcgc accggcctgg aaccgctcgg
     1201 ccgagcgcct ggccgcctcc gccgccttgt cgcccaatac cgtaatgaag ctgcgcagtt
     1261 ggccgtccac cgctccgatc ctcgcgaggt aggcttcggt caactcgacc gggtcgagct
     1321 cgccggtaag gaagccttgg aactgctctg ccgcgccgcg gtagacccac tcactcgccg
     1381 gcatcggcca ccaccctcgg ccactcggcc cacggctcga cgtcgaacgg ccggtaggcg
     1441 tcgtacgcca tcaggcccgc gaccagaccc cgcaggcgtt ccgtgatctc ctccaggtcg
     1501 ctgtcgggaa cccggtagcc ttgcgccgcg agcatggtgg cgacgtcggc ggtggagaac
     1561 tcggcagggt tgggctcagt cttccctgac atcgctcatc cccatccgac cccatgtgaa
     1621 cgtgagcggc tcacttgcgg cgtcaacccg cttcgatcca gcgggttccg gcgtggtcgc
     1681 gcggcacgac ggcgaaagcc cagtcgcccg gatgcacggg gtagttcctc gcccagtagc
     1741 ccaggtagcc gcccacgggc gcgcgcagct cctcgatggt ctcgaaggtg aaagggctga
     1801 tgaccttccc gagcagttcg ccggcctgca cctcgcgacc gaaggtgtcg cggcgccgct
     1861 ccggcttgag gtaaccgccg ttcctcgggt tgatgcgttg gacggtctcg tagacgagat
     1921 agcgcttcgg gcgttctatg gcgccctcct ccatgccgag gtggcgcatg acgttgcgga
     1981 tcccgcggta gttggcgtcc atccagctgt cctcgaggtc cgaatcgaag cccgccccgc
     2041 cgtggaagga gccgcaactg gggatcttca ggatctcgcc cgtataggcc tgagcggcgc
     2101 gcggccccgg gaacacgctc atcatcttcg cctggtacac gaggggg
//
LOCUS       DAOQYE010000341         2142 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_59084_length_2142_cov_3.062770, whole genome shotgun sequence.
ACCESSION   DAOQYE010000341 DAOQYE010000000
VERSION     DAOQYE010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59084_length_2142_cov_3.062770"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1830
                     /locus_tag="PKN52_06280"
     CDS             <1..1830
                     /locus_tag="PKN52_06280"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNQ99588.1"
                     /translation="DLARLGERGVVGEQLELSPEQRRAALASLPPPLTRLLHERIAAE
                     MRSDTNADQGALARHLLAAGEPGEAARRALAAATAALERTQLERATDLLYLALWSAQD
                     EPSLRLEACLALEGVAARRADYALQETLLAEVEQLAWEAQSDQALAEGGVRRARFMLT
                     RGKVGEALEAALKALEIALRVGDPALAARARNAVGAAHFYAGDLDGAAEAFAANLAGD
                     GEERYRAHNNLGSINAMRGRLNEAYQHFAAALTLARRDANRMDVSATLNNLGATAERL
                     GDYGQAIQHFREGIGLARRTSATRREAEMLVNLAVVYARQGQLGPAWNTTLEVEALGA
                     ELGDLRLALRAAEQRGEIERLCGDLERAMEDLAAAERLAEQIGDERKRKALEAQRLVI
                     RARLDPAAVGEAERLLEALESSGLKDVAPWLRLELASWAPDAGVARTQLARLGPDDLQ
                     SAHQHLVRDVALMRSGLLALQGLATTAPEARGDEAMPRPGAGSTLAVEDARRARARLV
                     AAGLLPGEADVLPAPQIVERPKARYLARYLARLLAAPATLAEGGDAVAGAAPAVPADV
                     LDELREQGAGLPRGLVEALLRTPSTWLPNPDDRALTPRALTPR"
     gene            1944..>2142
                     /locus_tag="PKN52_06285"
     CDS             1944..>2142
                     /locus_tag="PKN52_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99589.1"
                     /translation="MSDHSRRFLLKVWNEPGQGAGVERAWRATLRDVVDGSLHEFSAT
                     DELIGYLTSLGEAARVVGSNEE"
BASE COUNT          312 a          751 c          806 g          273 t
ORIGIN      
        1 gacctggcgc ggttgggcga gcgcggcgtc gtcggtgagc agttggagct ctctcccgag
       61 cagcgcagag ccgcgctcgc aagtctgccg ccaccgctca cgcggctcct ccacgagcgc
      121 atagccgcag agatgcgctc ggacaccaac gccgaccagg gcgcgctggc acggcacctg
      181 ttggccgcgg gcgagccggg tgaggcggcg cgccgcgctc tggctgccgc caccgccgcg
      241 ctcgagcgca cccagctcga gcgcgccacg gacctgctct acctggcgct gtggtcggct
      301 caggacgagc cgagcctcag gctcgaggcg tgcctcgcgc tggagggcgt cgcggcgcgc
      361 cgcgccgact acgcgctcca ggaaacgctc cttgcagagg tggagcagct cgcgtgggag
      421 gctcagagcg atcaggcgct ggccgagggc ggcgtgaggc gcgcccgctt catgctcacg
      481 cgcggcaagg tgggggaggc gctcgaggct gccctgaagg cgctcgagat cgccctgcgg
      541 gtgggcgatc cggcgctggc cgccagggcg cgcaacgccg tcggcgcggc gcacttctac
      601 gccggtgacc tcgacggggc cgcggaggcg ttcgccgcca acctggcggg ggacggcgag
      661 gagcgctacc gggcgcacaa caacctgggg agcatcaacg ccatgcgggg tcggctgaac
      721 gaggcttacc agcatttcgc cgccgctctc acgcttgccc ggcgcgacgc caaccgcatg
      781 gacgtgagcg ccacgctcaa caacctcggc gccaccgccg agcggctggg cgattacggg
      841 caggccatcc agcatttccg cgagggcatc gggttggcgc gccgcacgtc ggcgacgcgg
      901 cgcgaggcag agatgctcgt caacctggcg gtcgtctacg ccaggcaagg gcagcttggg
      961 ccggcatgga acacgacgct cgaggtcgag gcgctcgggg cggagctggg tgacctgcgg
     1021 ctggccttgc gggcggcgga gcaacgcggc gagatcgaac ggctctgcgg cgacctggaa
     1081 cgcgccatgg aggacctggc cgccgccgag cgcctggccg aacagatcgg cgacgagcgc
     1141 aagcgtaagg ccctggaggc ccagcggctc gtgatccgcg cgcgcctgga ccccgcggcc
     1201 gtcggtgagg ccgagcgcct gctggaagcg ctggagtcga gcggtctcaa ggacgtcgcg
     1261 ccgtggctgc ggctcgagct cgcgagctgg gcgccggacg ccggcgtggc gcggacccaa
     1321 ctcgcgcgtc tcggcccaga cgacctgcag agcgcgcatc agcacctcgt gcgcgacgtg
     1381 gcgctcatgc ggtccggtct actggcgttg caaggcctgg cgacgaccgc gcccgaagca
     1441 cgcggtgatg aagccatgcc tcgaccgggc gccggcagca cgctcgcagt cgaggacgcc
     1501 cggcgggcgc gggctcgcct agtggcagcg gggctcctgc cgggcgaagc ggacgtgcta
     1561 ccagcgccgc agatcgtcga acggccgaag gcgcgctacc ttgcgcgcta ccttgcgcgc
     1621 ctgctagcag ctcccgctac cctcgccgaa ggcggtgacg cggtcgccgg cgccgcgccg
     1681 gcggtacccg ccgacgtact ggacgagctc agggagcagg gcgctgggct tccgagaggg
     1741 ctggtcgaag cgctgctccg aaccccgtcc acctggctgc caaaccccga tgaccgtgct
     1801 ttgacacccc gtgctttgac accacgttga caccacctcc cctaacctgc tcccgacgtt
     1861 ggggcggcgc ccgctggcgc acccgtcgcc gccgccgcta gtggtggcgg gtgcggaccg
     1921 ccgaggcgtc gtgagggagt agcgtgagcg atcacagccg caggttcctg ctgaaagtct
     1981 ggaacgaacc tggccaagga gccggcgtgg agcgcgcctg gcgagcgact ctccgcgacg
     2041 tcgtggatgg ttcgctgcac gagttctccg ccacggacga gctgatcggg tacctgacca
     2101 gcctgggcga ggcggcacgc gtcgtcggtt cgaacgagga gg
//
LOCUS       DAOQYE010000342         2140 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_59167_length_2140_cov_3.066667, whole genome shotgun sequence.
ACCESSION   DAOQYE010000342 DAOQYE010000000
VERSION     DAOQYE010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59167_length_2140_cov_3.066667"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            486..623
                     /locus_tag="PKN52_06290"
     CDS             486..623
                     /locus_tag="PKN52_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99590.1"
                     /translation="MLGTDSNIYLDPQPTREEKEALYRQVERETIAIATAAVDLLHAE
                     A"
     gene            620..>2140
                     /locus_tag="PKN52_06295"
     CDS             620..>2140
                     /locus_tag="PKN52_06295"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNQ99591.1"
                     /translation="MTPNAPSLTERDLLVTGATLVDVVTQSTYRGWFTVWYGRFVEVE
                     AGEPSEADLAGLSIAERRDLGGAHVQPGMLDVHMHIESSLVTPRRFAEAALPHGTTTI
                     LQDPHEVANVLGDPGIAAMRAAARGLPQRVYTAVSSCVPATSADIETPNAAITPAEVT
                     ELARDPAVIALGEVMDYQGLVAGDPHLRAMVAAAQAAGLSVEGHVPSLAGAALSRYVA
                     HGVRSDHTLATPAKLLEELRKGLWVMIQEKSLTPDVVREIVALPDRSRVILITDDVMP
                     NRLLSGHLSRLVALAVGLGWPTLDAIASATLRPATYLGLGGLGLIAPGAHADYLVTDG
                     LAVYPPLETHVGGRLVARAGETVAACLTAGLPRSGQAYAAANVAREALGFGGHEERAD
                     ARHVAHEPRSTGGERSSAAAARRTLRAIVRARVITVNKVNSFTTLREADVELEDGVPT
                     DPDIALACVVPRAALLPGAGAYAPIVCLVAGTGLRDGGYASTFAHDSHNVFLFGRSP"
BASE COUNT          300 a          826 c          715 g          299 t
ORIGIN      
        1 aagctgatcg cgcgcaaccg cgagtacacg ctcttcacgg gccgcacggc cgccggcacg
       61 cccatcagcg tctgctcgac cggcatcggc ggaccgtcgg cgtccatcgc cgtcgaggag
      121 ctcgtccgga tcggggccac gcacttcatc cgcgtcggct cggcgggcgg caggcagccc
      181 ggcacgccga tcggctcggt ggtagtcgtg acggcggcct tcaggggcga ggggacgtcc
      241 ctcgactacg tcccgctcgg ttaccccgcg gccgccgacc tcgacgtcac gctcgccctg
      301 cgccgcgccg ccgagacgta ccttggcagg cgcgccatcg agggcgtcgt ctacacgcgc
      361 gacgccttct atcgccgcga cgacggcctc aaccagcgcc tcacggaggc cggcgtggtc
      421 gcggccgagc aggagtgctc caccgtcttc gtcgtcgggg ccctcctcgg ggtgaggttg
      481 gcgccgtgct cggcacggac tccaacatct acctcgaccc gcagccgacc cgtgaggaga
      541 aggaagcgct ctaccgccag gtcgagcgcg agacgatcgc catcgctacc gccgccgtgg
      601 acctgctcca cgctgaggcg tgacgcccaa cgcacccagc ctgaccgagc gcgacctgct
      661 cgtgaccggc gcgacgctcg tcgacgtagt cacgcaatcg acctatcgcg gctggttcac
      721 cgtctggtat ggccggttcg tcgaggtcga ggcgggggag ccctccgaag ccgacctagc
      781 cggcttgagc atcgccgagc gccgcgacct cgggggcgcc cacgtgcagc cgggcatgct
      841 cgacgttcac atgcacatcg agagcagcct cgtcacgccc cgacgtttcg ccgaggccgc
      901 cctcccgcac ggcacgacca ccatcctgca ggacccgcac gaggtcgcca acgtgttggg
      961 ggatccaggc atagccgcga tgcgcgcggc cgcgcgcggc cttccacagc gcgtctacac
     1021 ggccgtgtcg agctgcgtcc ctgcgacatc ggccgatatc gagacgccga acgccgccat
     1081 cacgccggcc gaggtgacgg agctggcgcg cgatcccgcg gtcatcgccc tcggggaggt
     1141 catggactac cagggcctag tcgcgggcga ccctcacctc cgggccatgg tcgccgcggc
     1201 gcaggccgcc ggcctcagcg tcgagggcca cgtgccgagc ctcgcgggcg ccgccctctc
     1261 gcgctacgtg gctcacggcg tgcgctccga tcacactctc gccacgcccg ccaagctcct
     1321 cgaggagctc cgcaagggcc tatgggtgat gatccaggag aagtcgctca cgcccgacgt
     1381 cgtgcgggag atcgtcgccc tgcctgatcg cagccgcgtc atcctcatca ccgacgacgt
     1441 catgcccaac cggctcctga gcggccacct gagccgcctc gtggcgctgg ccgtcgggct
     1501 cggctggccg acgctcgacg ccatcgcgtc cgccacgttg cgacccgcca cgtacctggg
     1561 tctgggcggg ctcggcctca tcgcgcccgg cgcccacgcc gactacctcg tcacagacgg
     1621 acttgccgtc tacccaccgc tggagacgca cgtcgggggc cgcctcgtcg ctcgggcggg
     1681 agagaccgtt gcggcctgct tgacggccgg gctgccgagg tccggacagg cgtacgcggc
     1741 cgccaacgtg gcgcgcgagg cgcttggctt cggcgggcac gaggagcgtg cggacgcgcg
     1801 ccatgtcgcc catgagccac gttcgaccgg cggagagcgt tcgtccgccg cggccgcacg
     1861 ccgaacgttg cgcgcaattg tgcgcgcccg cgtcataacg gtcaacaagg tcaacagctt
     1921 cacgacgttg cgggaagcgg acgtcgaact ggaggacggc gtgccgaccg acccagacat
     1981 cgccctcgcc tgcgtcgtcc cccgcgccgc gctgctgccc ggcgccggcg cctacgcgcc
     2041 catcgtctgc ctggtcgctg gcacgggcct gcgcgatggc ggctacgcct cgaccttcgc
     2101 ccacgacagc cacaacgtct tcctcttcgg gcgcagcccc
//
LOCUS       DAOQYE010000343         2139 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_59214_length_2139_cov_2.720250, whole genome shotgun sequence.
ACCESSION   DAOQYE010000343 DAOQYE010000000
VERSION     DAOQYE010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59214_length_2139_cov_2.720250"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1033
                     /locus_tag="PKN52_06300"
     CDS             <1..1033
                     /locus_tag="PKN52_06300"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HNQ99592.1"
                     /translation="AGIALLYSHGQSANDWVTGKLTGMTPAEIKKEHDLKSSRFGGKA
                     AREFTTKYKANTTDFDAKTRQVSSKLAHIHKTRVEPKVKITGPGNKTVTQEAQGAQDA
                     IRAVLAKDLGQSVRISFGFGGTGGGTGAGLGFSGALRGFQAAQLAKQQIGDSYAIGAT
                     GPNVFDCSGLVKYVYNRSGLPGFPTYTGNQWPLGRQVAKDALMPGDQLFMKTGIPEWT
                     DQFGWGHTGIYVGNGMYVHASGKRTGVIMSSVASIPYPWAAKRHFFASGGLIKAQPGG
                     VNAVLGEAGEDELVITKLRSAEPRGSDGASLAGVSYVVHMHNPVFMSGEDAIGEMERA
                     ARRRARVRA"
     gene            1030..>2139
                     /locus_tag="PKN52_06305"
     CDS             1030..>2139
                     /locus_tag="PKN52_06305"
                     /inference="COORDINATES: protein motif:HMM:NF016013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF389 domain-containing protein"
                     /protein_id="HNQ99593.1"
                     /translation="MIERLADLDLHTAPYSVAEREWGPAFGEERQVRYLIEVAGATQD
                     ACTAAITRLLSRLPSIIGPSELVLEPTGGNRPMALTALRTISAAEPYTHAQDIGFRAD
                     VEFALAVTPTTHRANRVYLAAPIGDEFPSHADVARSCNGAGEAARYVYADGGRWVVTA
                     SASDRGILDLYADVVADEQLVVEFDYDVVAAGATLNVTLELPQTGAGTYTWTLLSKAT
                     TGAGRFIAAYRVPAFDAGERAYLRATWSANSASTAYLENVRIGAKTVANERLLPGAPS
                     DAESTLPYGWSAQAAGNVTYSYGSGKFQLAANVASAAACLAYGTQGVTYATHAAAGTA
                     GMLSLTNAKSGALVGVLISVTTIPAAANVAIAAAFS"
BASE COUNT          340 a          786 c          682 g          331 t
ORIGIN      
        1 cgccggaatc gcgctgctct attcacacgg ccagtcggct aacgactggg tcaccggcaa
       61 gctcaccggt atgacgccgg ccgagatcaa gaaggagcac gacctcaagt catcccgctt
      121 cggtggcaag gccgcgcgcg agttcacgac caagtacaag gccaacacaa ccgacttcga
      181 cgccaagacc aggcaggtct ccagcaagct cgcacacatc cacaagactc gggtcgagcc
      241 caaggtcaag atcaccggac ccggcaacaa gaccgtcacc caggaggcgc agggtgcgca
      301 ggatgcgatc cgcgccgtgc tggctaagga tctgggccag tcggtgcgca tctccttcgg
      361 gttcggcggc actggcggcg gcaccggcgc cgggctcggt ttctccggcg cgctgcgcgg
      421 cttccaggcg gcgcagttgg ccaagcagca gatcggcgac tcctacgcga tcggcgccac
      481 cggccccaac gtctttgact gctcgggcct ggtgaagtac gtctataacc gctccggcct
      541 gcccggcttc ccgacctaca ccggcaacca gtggccgctc ggacgtcagg tcgccaagga
      601 tgcgctgatg ccgggcgatc agctgttcat gaagacgggg atcccggagt ggaccgacca
      661 gttcggctgg gggcataccg ggatctacgt cggtaacggc atgtacgtgc atgcctccgg
      721 caagcgcacc ggcgtgatca tgtcctcggt cgcctcgatc ccctacccct gggcggcgaa
      781 gcgccacttc ttcgcctcgg gcggcctgat caaggcccag cccggcggtg tgaacgcggt
      841 gctcggcgag gccggcgagg acgagctggt gatcaccaag ctacgctcgg ccgaaccgcg
      901 cggcagcgac ggtgcttcgc tggccggcgt ctcttacgtc gtgcatatgc acaacccggt
      961 cttcatgagc ggcgaggatg ccatcggtga gatggaacgc gccgcccgcc gccgcgcgag
     1021 ggtgcgggca tgatcgagcg tctcgccgac cttgacctgc acaccgcgcc ctactcggtc
     1081 gctgagcgcg agtggggacc ggccttcggt gaggaacgtc aggtccgcta cctgatcgag
     1141 gtcgccggcg ccacgcagga tgcctgcacg gccgccatca cgcgcctgct ctcgcggctg
     1201 ccgtcaatca tcggcccgtc cgagcttgta ctcgagccga ccggcggcaa ccgcccgatg
     1261 gcgctcaccg cgctgcggac catctcggcc gcggagcctt acacccacgc gcaggacatc
     1321 ggcttccgcg ccgacgtcga gttcgctctc gccgtcacac cgacgaccca ccgcgccaac
     1381 cgtgtctacc tcgccgcccc cattggcgat gagttcccct cccacgccga cgtggcccgc
     1441 tcctgcaacg gcgccggaga ggccgcccgc tacgtctatg ccgacggtgg gcgctgggtc
     1501 gtgaccgcct ccgcctccga ccgcggcatc ttggatctct acgccgatgt cgttgccgat
     1561 gagcagctgg tggttgagtt cgactatgac gtcgtggccg ccggcgcaac cctcaatgtc
     1621 acgctggagc tgccccagac cggcgccggc acctacacct ggacgctgct ctcgaaggcg
     1681 accaccggcg cgggccgctt catcgccgcc taccgcgtgc ccgccttcga tgccggcgag
     1741 cgcgcctatc tgcgcgcgac ctggtccgcc aactcggcct cgacggcgta tctggagaac
     1801 gtgcgcatcg gcgccaagac ggtcgccaac gagcgcctgc tgccgggcgc gccttcggat
     1861 gctgagtcga cgctgcccta cggctggagc gcacaggcgg ccggcaacgt cacctactca
     1921 tacggctccg gcaagtttca gcttgccgcc aacgtggcca gcgctgccgc ctgtcttgcc
     1981 tacggcacgc agggcgtcac ctatgcgacc cacgccgccg ccgggaccgc ggggatgctc
     2041 tcgctcacca acgccaagtc cggcgccctt gtcggggtcc tgatctccgt caccacgatc
     2101 ccggcggcgg ccaacgtcgc gatcgcagcg gcgttcagc
//
LOCUS       DAOQYE010000344         2130 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_59633_length_2130_cov_3.181687, whole genome shotgun sequence.
ACCESSION   DAOQYE010000344 DAOQYE010000000
VERSION     DAOQYE010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59633_length_2130_cov_3.181687"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1534
                     /locus_tag="PKN52_06310"
     CDS             <1..1534
                     /locus_tag="PKN52_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99594.1"
                     /translation="LQRLARRVWWDDLEQYVQRVAAGWRAERDRFTARLVFATLQNLQ
                     RNADRSTFGQDPGPRAFPVIEPVPTMNDPLVSLSDLDSLAEIVRELINVVMTSGKVGN
                     PLPPLERGESGAFAYVKSAALTVANDPYAGRMAPDTNRSVTSTQLRLALQELGKERLP
                     EEERAAQRRDLERRLVEVSAKERSDRQAFQRDAAMFTELVEAFFARLAGYLPTSVGGE
                     APGPQLEGGILFGVNPILRWDRVPPGAAAVTIRMVGPLRLSLAGHDLAIAGTGKARTL
                     YLDGEELELEGSGIIQRDRVRLGAFREGDYVHLRIRNDARSLGSRLADALVVNSVLTS
                     PQRSELLAIFKVIANNVRGEPSEVVDQAVARANVVAARAPDKAKALDGLLRGAARAAG
                     VLVSEPVMRETVARLVGVDGAAVTDVAAIVAGSGATEHQVKTITDEPMTFDLANLKIT
                     VRQYRGRGRTAQESLVAMLPGQVLGSFADYLLVPRPNGTLLFARGEEEVAVLFLPAYV
                     SA"
     gene            complement(1573..>2130)
                     /locus_tag="PKN52_06315"
     CDS             complement(1573..>2130)
                     /locus_tag="PKN52_06315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99595.1"
                     /translation="DAGGPLLLSELAADAAGVWEAAAPGTAVFSQDPAAITVVRYGPD
                     ELLGEALSLTLQRSGRIEVLVSPTAGASVKAALVHELGILLGLPEGGAGIMARALAAD
                     VATPTPAEVAELLGRRAYPPMDLDRSGAVDFYDLAAFGRAYGQTGVNLAADFNGDGKV
                     DGADLELLREAYEFGAPSRTAPPRN"
BASE COUNT          311 a          758 c          759 g          302 t
ORIGIN      
        1 actgcaacgg ctcgccaggc gcgtctggtg ggacgacctc gagcagtacg tccagcgcgt
       61 ggccgccggt tggcgcgccg agcgcgaccg gttcaccgcc aggttggtct tcgcgacgct
      121 tcagaacctc cagcgcaacg ccgaccgcag cacgttcggg caggaccccg gccctcgcgc
      181 gttcccggtc atcgaacccg tcccgaccat gaacgatccg ctcgtgtcgc tttcggacct
      241 ggattccctg gccgagatcg ttcgcgagct catcaacgtc gtcatgacga gcgggaaggt
      301 cggcaacccc ctgccgccgc tcgagcgcgg cgagtcgggg gccttcgcgt acgtcaagag
      361 cgccgccctg acggtggcga acgacccgta cgccggccgc atggctcccg acacgaaccg
      421 gtcggtcacg tcgacgcagc tccgtttggc gctccaggag ctcggcaagg agcgcctccc
      481 ggaggaggag agagcggccc agcgtcgcga cctcgagcgg aggctcgtcg aggtaagcgc
      541 caaggagcgt tccgaccgcc aggcgttcca gcgcgatgcc gccatgttca ccgaactcgt
      601 ggaggcgttc ttcgcgcgtc ttgccggcta ccttccgacg agcgtcggcg gcgaggcccc
      661 ggggccgcaa ctcgagggcg gcatcctgtt cggcgtcaac cccatcctgc gctgggacag
      721 ggtgcccccc ggcgccgcgg ccgtgacgat ccgcatggtc ggcccgctgc gcctctccct
      781 ggccggtcac gacctcgcca tagccggcac aggcaaggcc aggacgctgt acctggacgg
      841 agaggaactc gagctcgaag gctccggcat catccagcgc gaccgcgtgc ggctcggggc
      901 gttcagggag ggggactacg tccacctgcg catccgcaac gacgcgcgtt ccctcggctc
      961 acgccttgcc gacgcgctcg tcgtgaacag cgttctcacg agcccgcagc gaagcgagct
     1021 gctggccatc ttcaaggtca tcgccaacaa cgttcgtggg gagccgagcg aggtcgtcga
     1081 ccaggcggtc gccagggcca acgtcgtggc ggcacgcgcg cccgacaagg cgaaggcgct
     1141 cgacggtctg ctacgcggcg ccgcgcgcgc cgcgggggtc ctggtctccg aacccgtgat
     1201 gcgcgagacg gtcgcgcgcc tggtcggcgt cgacggggct gccgtcacgg acgtggccgc
     1261 catcgtcgcc ggttcaggcg cgacggagca ccaggtcaag acgataaccg acgagcccat
     1321 gaccttcgac ctcgccaacc tcaagatcac cgtcaggcag taccgcggtc gtggccgcac
     1381 ggcacaggag agcttggtgg ccatgctgcc tggacaggtg ctcggttcgt tcgcggacta
     1441 cctgctcgtg ccgcggccga acggcacgct cctgttcgcg cggggcgagg aggaagtggc
     1501 cgtgctcttc ctgcccgcct acgtctcggc ctgagcgctg ccaggaacgg tccgccggac
     1561 gagcgcgctc agttagttgc gtggtggagc cgtgcgcgac ggcgccccga actcgtaggc
     1621 ctctctcagc agctccaggt cggcgccgtc caccttgccg tcgccgttga agtcggccgc
     1681 gaggttgacg cccgtctgcc cgtaggcgcg gccgaaggcc gccaggtcgt agaagtcgac
     1741 cgcgccgctc ctgtccaggt ccatcggggg gtaggcgcgt ctgcccagca gctcggcgac
     1801 ctcggccggg gtaggggtgg cgacatcggc ggccagtgcg cgggccatga tccccgcgcc
     1861 gccctccggc agaccgagga ggatgccgag ttcgtggact agggccgctt tgacgctcgc
     1921 gccagccgtc ggggagacga ggacctcgat gcggccggag cgctggaggg tgagcgacag
     1981 cgcctcccct aggagctcgt cggggccgta gcgcacgacg gtgatggcgg ccgggtcctg
     2041 cgagaagacc gcggtgcccg gcgcggccgc ctcccagacg ccggccgcgt ccgcggccag
     2101 ctcagagagg agcagggggc cgccggcgtc
//
LOCUS       DAOQYE010000345         2128 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_59726_length_2128_cov_2.963338, whole genome shotgun sequence.
ACCESSION   DAOQYE010000345 DAOQYE010000000
VERSION     DAOQYE010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59726_length_2128_cov_2.963338"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="PKN52_06320"
     CDS             <1..311
                     /locus_tag="PKN52_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNQ99596.1"
                     /translation="QSRLADMLSEITRGSLLAYRLGRLKEEGKDHPARVSLAKRDNCR
                     SALAVARAARDLLGGNGITTEYAAIRHMLNLETVATYEGTDTVHTLVLGREITGMNAF
                     "
     gene            complement(358..675)
                     /locus_tag="PKN52_06325"
     CDS             complement(358..675)
                     /locus_tag="PKN52_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99597.1"
                     /translation="MRVVLMLVALGLVVVNTFGAWAVSRRKPSVARLFLLAAMVLTVA
                     LVAYGFADPIAWWVLLAGTTLGFLASFLNARLVIGKVVWPYHLLRAAVLAALLALARV
                     LGG"
     gene            complement(680..1540)
                     /locus_tag="PKN52_06330"
     CDS             complement(680..1540)
                     /locus_tag="PKN52_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99598.1"
                     /translation="MRIGFLTQLSWRRYGRYWVELALAAGLEPAFADPEAVKEAWQDP
                     DLSGVPTLAFRLAAAQALALADCQAIVVPRLARESGVERGSGQDPWVMDLAARLRSAL
                     PGLPTLHAVPAWFDESLESESVTFLQTAIHDAMSVRRALGRVRPLAKAGKAGVPDLAR
                     RGGTRTVAVLGRPWVVDADLARLVQGEGERTVGQHLFDPEELRSEGWRADERLVDGDA
                     EVIGAARLLGRRAAVDALRLVVDEEASSDAWLANRVAKVVHKPLEVVTLQAVLTGRDP
                     VDTLLRGPVD"
     gene            complement(1561..>2128)
                     /locus_tag="PKN52_06335"
     CDS             complement(1561..>2128)
                     /locus_tag="PKN52_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1998 domain-containing protein"
                     /protein_id="HNQ99599.1"
                     /translation="VGAPPPGIHVGRVRVTHTVTGYVRKRFFSEAALDERPLDLPEVA
                     YETHAMWFEATGLVSPPAAADMPAALHALEHTLIGLLPAFVLCERADVGGVSYPVYPA
                     TGAPTIFIYDGHPGGVGYVRAGAAVFSTWLTAAAALLAGCECKGGCPRCVLSPKCGNG
                     NQYLDKAAAEVLARAMLVRLAPVAGAAA"
BASE COUNT          348 a          772 c          756 g          252 t
ORIGIN      
        1 tccagtcgcg cttggcggac atgctctcgg agatcacgcg cggcagcctc ctcgcttacc
       61 gcctcgggcg gctcaaggag gagggcaagg accacccggc gcgcgtctcg ctcgccaagc
      121 gcgacaactg tcgcagcgcg ctcgccgtgg cgcgcgccgc gcgcgacctg ctcggcggga
      181 acggcatcac gacggagtac gcggccatcc gccacatgct gaacctcgaa acggtggcga
      241 cctacgaggg taccgacacg gtccacaccc tcgtgctcgg ccgcgagata acggggatga
      301 acgcgttctg acgaccgtga ccggaggccg gtcccggcgg gacgaccggc cgcggcgtca
      361 gccgccgagt acgcgcgcca gcgcgagcaa cgcggcgagg acggccgctc tcaacagatg
      421 gtacggccag accactttgc cgatcacgag gcgcgcgttc aggaaagacg ccaggaagcc
      481 gagcgtggtg cccgcaagga gcacccacca ggcgatgggg tctgcgaaac cgtaagccac
      541 gagggccacc gtcaggacca tggcggctag gaggaacagt cgcgccacgg agggcttacg
      601 cctgctcacc gcccaggcgc cgaaggtgtt gaccaccacg aggccgagcg ccaccagcat
      661 caggacgacg cgcacgtcgt cagtctacgg gcccgcgcag cagggtgtcg acggggtcgc
      721 ggccggtcag gacggcctgg agcgtcacga cctcgagggg cttatggacg actttcgcca
      781 cgcggttggc caaccaggcg tcgctgctcg cctcctcgtc gaccaccaac ctcagagcgt
      841 ccacggcggc gcggcgacca aggaggcgcg ctgctccgat gacctcggca tcgccgtcca
      901 cgaggcgctc gtctgcgcgc cagccctcgg agcggagctc ctccgggtcg aagaggtgct
      961 gaccgaccgt ccgctcgcct tcgccttgga cgagccgcgc caggtcggcg tcgacgaccc
     1021 acggtcgccc gagcacggcc acggtgcgcg tgccaccgcg tctagccagg tcgggaaccc
     1081 cggccttgcc ggccttggcc agcggcctga cgcgtccgag cgcccgtctg accgacatcg
     1141 cgtcgtggat cgccgtctgg aggaacgtga ccgactcgga ctcgagcgac tcgtcgaacc
     1201 atgccggcac ggcgtgcagc gtaggcaacc ccggcagcgc ggagcggagc cgggcggcga
     1261 gatccataac ccacgggtcc tggccagagc cgcgctccac gccggactcg cgcgcgagcc
     1321 ttggcaccac gatggcctgg cagtccgcca gcgccagcgc ctgggccgcc gcgaggcgga
     1381 acgccagggt agggaccccg ctcagatcgg ggtcctgcca ggcctccttg accgcctccg
     1441 ggtcggcgaa agccggctcc aggcccgcgg ccaacgccag ttccacccag tagcggccgt
     1501 agcggcgcca ggagagctgc gtcaggaatc cgatgcgcac gaggcgcgag tctaccatcc
     1561 ctacgccgcg gcaccggcga cgggcgccag gcgtaccagc atggccctgg ccagtacctc
     1621 ggcggcggcc ttgtcgaggt actggttgcc gttgccgcac ttcggcgaga gcacgcagcg
     1681 cgggcaaccg cccttacact cgcagccggc caggagcgcg gcggcggcgg tcagccaggt
     1741 ggagaagacc gcggcgcccg cgcgcacgta gccgacgccg cccggatggc cgtcgtagat
     1801 gaagatcgtc ggcgctcccg tggccgggta gaccgggtac gacacgccgc cgacgtccgc
     1861 ccgctcgcag agaacgaagg cgggcagaag cccgatcagg gtgtgctcga gcgcatgcag
     1921 cgcggcgggc atgtccgcgg ccgccggtgg cgagacgagg cccgtcgcct cgaaccacat
     1981 ggcgtgagtc tcgtacgcga cctctggcag gtcgagcggg cgctcgtcca gcgcggcctc
     2041 gctgaagaac cgcttgcgca cgtagccggt caccgtgtgc gttacccgca cgcgcccgac
     2101 gtggataccg ggaggcgggg cgccgacg
//
LOCUS       DAOQYE010000346         2124 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_59873_length_2124_cov_3.670855, whole genome shotgun sequence.
ACCESSION   DAOQYE010000346 DAOQYE010000000
VERSION     DAOQYE010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59873_length_2124_cov_3.670855"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1104
                     /locus_tag="PKN52_06340"
     CDS             <1..1104
                     /locus_tag="PKN52_06340"
                     /inference="COORDINATES: protein motif:HMM:TIGR04350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HNQ99600.1"
                     /translation="DVLPLWIAEMDYPLAQPIKDAIAARLDADDLGYPAAAAYPTAAG
                     LLGLREAVVERLASRYGLRVAPEQIEMLSSTSAGFGLAARAVAGPGDEVLLLTPLYPP
                     FKHAIETVGRVPVEVELVRGDDGYTIDFAALEAAVTPATRIIMLCSPHNPIGKVFTRS
                     EVEQLADFARRHNLWVVSDELHADLLFDGKHVAFATVSDDAARRTVTLYGPTKAFNIP
                     GLRISFAASANAGLLKRMKAASEGLSPSPNSLAMAATVAAYRDGGAWLDDTLSYLKGN
                     RDYVVSFVRERLPQVGVHVPAATYLAWLDMRRTGLGDTPGKALMEQAKVGLNEGSSFG
                     KGGAGFVRLNFATSRAILSEAMERIAAVLAPRD"
     gene            1152..>2124
                     /locus_tag="PKN52_06345"
     CDS             1152..>2124
                     /locus_tag="PKN52_06345"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HNQ99601.1"
                     /translation="MSQADIQTLRLAPGLEAAVGSGHPWVYRNHVPANTLRGGEWVRI
                     EAGRAAAYGLYDADGAIAVRLYGAGRPASGWVGARVADALALRDRLPGTGTDAYRLIY
                     GEGDYLPGVVCDRYGRYAVMKRYSASVDPLLTEVAKEVGTRLKLKGVALRSEVEGDGA
                     AAERESHGLSALWGELPPPELTVSENGLRFKMDPWHGQKTGMFLDQRDNRQLVRGLAA
                     GKSVLNLFAYHGGFSVYALAGGAASVVSVDVSRAALEAAEENVRLNAPTGEHSAMVAD
                     IFVSLPVWAEAGPTHDIVVLDPPSLANASTQRRRAQRAYLKLNRDAFR"
BASE COUNT          318 a          761 c          735 g          310 t
ORIGIN      
        1 gacgtgctgc ctctctggat cgccgagatg gactaccccc tcgcccagcc gatcaaggac
       61 gccatcgcgg cccgcctgga cgcggacgac ctcggctacc ccgcggcggc ggcttatccg
      121 accgcggcgg gcctgctagg cctccgcgag gccgtggtcg aacgcttggc ctcccggtac
      181 ggcctaaggg tggcgcccga gcagatcgag atgctttcca gcaccagcgc aggcttcggc
      241 ctcgcggcac gcgccgtggc cgggccggga gacgaggtcc tgctcctcac ccccctctat
      301 ccgccgttca agcacgcgat cgagaccgtc ggtcgggtgc cggtcgaagt cgagctcgta
      361 agaggagacg atggctatac catcgacttc gcggcgctgg aggcggccgt cacccccgcc
      421 acacgcataa tcatgctctg cagcccgcac aaccccatcg gcaaggtgtt cacgcgctcc
      481 gaggtcgagc agctggccga cttcgccagg cggcacaacc tctgggtcgt gtctgacgag
      541 ctgcacgccg acctgctctt cgacggcaaa cacgtcgcct tcgccaccgt gtccgacgac
      601 gcggcccgac gcaccgtcac gctctacgga ccgaccaagg cgttcaacat ccctggcttg
      661 cgcatctcgt tcgccgcctc cgccaacgcg ggcctgctga agcgcatgaa ggcggccagc
      721 gaggggttgt cacccagccc caactcgctc gccatggccg cgaccgtggc ggcctaccgc
      781 gacgggggcg cgtggctgga cgacactctc tcttacctga agggcaaccg cgactacgtc
      841 gtgagcttcg tgcgcgagcg tctcccgcag gtaggcgtgc acgtcccggc ggcgacgtac
      901 ctggcctggc tcgatatgcg gcgcaccggg ttgggcgaca cgcccgggaa ggcgctgatg
      961 gagcaggcca aggtgggtct caacgagggc agcagcttcg ggaagggcgg cgctgggttc
     1021 gtgcgcctca acttcgccac ttcgcgcgcc atcttgagcg aggcgatgga gcgcatcgcc
     1081 gccgtcctcg cgccgcgcga ctgagcggaa ggctcaaggc cggccttggt ccccctcgag
     1141 cgtgtgagca gttgagccag gccgacatcc agacgctccg cctggcgcca ggcctggaag
     1201 ccgcggtcgg ctcaggccac ccgtgggtct accggaacca cgtgccggcg aacacgctgc
     1261 gcggcggcga gtgggttcgg atcgaagccg gccgggccgc ggcgtacggg ctttacgacg
     1321 ccgacggcgc gatcgcggtg cgcctgtacg gagccggtcg gcccgccagc ggttgggtcg
     1381 gcgcccgcgt cgccgacgcc ctcgccctgc gggaccgcct gcctggcacc ggcaccgacg
     1441 cctaccgcct catctacggc gaaggggact acctgccagg cgtcgtgtgc gatcgctacg
     1501 ggcgctacgc cgtcatgaag cgctactccg cctccgtcga tcccttgctg accgaggtgg
     1561 ccaaggaggt cggaacgcgc ctgaagctga agggcgtcgc gctgcgctcg gaggtcgagg
     1621 gcgacggcgc cgctgccgag cgcgagagcc atggcttgag cgcgctctgg ggtgagcttc
     1681 cgcctccgga gctcaccgtc agcgagaacg ggctgcgctt caagatggac ccgtggcacg
     1741 gccagaagac ggggatgttc ctcgaccagc gcgacaaccg ccagctcgtc aggggtctgg
     1801 cggccggcaa gtccgttctc aacctgttcg cctatcacgg ggggttcagc gtctacgcgc
     1861 tggccggagg cgcggcttcc gtcgtcagcg tcgacgtgtc gcgagcggcg ctggaggcgg
     1921 ccgaggagaa cgtgcgcctg aacgcgccga cgggggagca tagcgccatg gtggcggaca
     1981 tcttcgtgtc gctaccggtc tgggccgagg cgggacccac ccacgacatc gtggtgctcg
     2041 acccgccctc cctcgcgaac gcgagcacgc agcggcgccg ggcgcagcgc gcctacctga
     2101 agctcaaccg cgacgccttc cggc
//
LOCUS       DAOQYE010000347         2123 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_59939_length_2123_cov_2.963733, whole genome shotgun sequence.
ACCESSION   DAOQYE010000347 DAOQYE010000000
VERSION     DAOQYE010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59939_length_2123_cov_2.963733"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1029)
                     /locus_tag="PKN52_06350"
     CDS             complement(<1..1029)
                     /locus_tag="PKN52_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99602.1"
                     /translation="MRSLRALLEAAKLGRGALAALAWLVAGSLGRAATTVLAVPLIVG
                     LAGRTPDTWWYLAAFLAALGVGAVFDLRASLAAFDVGLGVAAHHDSVLLGRVRAAPLA
                     WFTPERRQRLEQMFSSGGVELAQGFAHLLAPLFDALFTTTFVALGVFLFAPGLAWVLL
                     GLVALHLGAFVLQVVLTRSSERRWFEAAERFSRALAEFSRLQPLLRLSAQGAGSRLDD
                     AAERQRAAARTLAVTSVAGHQAVTVVRVAALVLIPLQVVVSLASGAISAFEAAALIIV
                     AARFLDPLMQLDRLGATLTGAIAILTRLDEVFEAPATRTVALPVPRPAAAAAAPAVRL
                     DNVGFRYPG"
     gene            complement(1026..>2123)
                     /locus_tag="PKN52_06355"
     CDS             complement(1026..>2123)
                     /locus_tag="PKN52_06355"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ99603.1"
                     /translation="IQVDRLFPTPAFADLLEERQTFFQRLQRPTARLRALTDIVVRPA
                     TLFGMYVLVGVPLVMAGALGLGRLVPFLVLAPAFGTSILGLLYTAAAVREANAAARRI
                     GLLLEEPPLAPPASTAPLPLRSGRLRFESVTFGYHPRRPVVRDLSLDLEPGTVTALVG
                     RSGAGKSTVAALAARLHDPQGGVVTLDGRPIADYPAAELGRRLAFVFQQPQMLDDTVA
                     ANLRLARLDATDAELARVLGAAQLAGVVAALPDGLATRLGGAFRLSGGETQRLAIARL
                     LLTNPDVIVLDEATAYADPESEALMQRAISELVAGRTVLVIAHRLQTIANAHQIVVVE
                     DGVVAERGTHGELLAANGIYARLWGEAGVAA"
BASE COUNT          330 a          749 c          811 g          233 t
ORIGIN      
        1 cccggggtag cggaagccga cgttgtcgag gcggacggcg ggggcggccg ctgccgcggc
       61 cggtcgcggc acgggtagcg ccaccgttcg cgtcgccggg gcctcgaaca cctcgtccaa
      121 ccgcgtgagg atggcgatgg cgcccgtgag cgtcgccccc aagcggtcga gttgcatcaa
      181 cgggtcgagg aaccgggcgg ccacgatgat gagcgccgcc gcctcgaacg ccgagatggc
      241 gccgctcgcc aaggagacga cgacctgcag ggggatcagc acgagcgcgg ccacgcgcac
      301 gaccgtcacg gcctggtggc ctgccacgct cgtgaccgcc aaggtacgtg cggcggcgcg
      361 ctggcgctcg gccgcgtcgt cgaggcgcga ccccgcgcct tgggcggaca ggcgcaggag
      421 cggctggagg cgcgagaact ccgccagggc gcggctgaag cgctcggcgg cctcgaacca
      481 gcggcgctcg ctgctccgcg tgagcaccac ctgcagcacg aaggcgccca ggtggagcgc
      541 caccaacccg agcagcaccc aggcgagccc gggcgcgaag aggaacactc ccagcgcgac
      601 gaacgtcgtc gtgaacagcg catcgaagag cggcgccagg aggtgcgcga aaccctgcgc
      661 cagctcgacg ccgccgctcg agaacatctg ctcgagccgc tggcgcctct ccggcgtgaa
      721 ccaggccaac ggggccgcac ggacgcggcc gagcagcaca gagtcgtggt gagcggccac
      781 cccgaggccg acgtcgaacg cggccaagga agcgcgcagg tcgaagacgg cgccgacgcc
      841 gagcgcggcg aggaaagcgg ctaggtacca ccaggtgtcc ggggtccgcc ccgcgaggcc
      901 gacgatgagc ggtaccgcga gcacggtggt ggcggcgcgc ccgagcgacc ccgccacgag
      961 ccaggcgagc gccgccaacg cgccgcgccc gagcttcgcg gcctccaaga gcgcccgcaa
     1021 gctcctcacg ccgccacccc cgcctcgccc cacaaccgcg cgtagatgcc gttggctgcg
     1081 agcagttcac cgtgtgtgcc gcgctcggcc accaccccgt cctccaccac cacgatttgg
     1141 tgagcgttgg cgatcgtctg gagccggtgc gcgatgacga gcaccgtcct ccccgcaacg
     1201 agctcgctga tggcgcgctg catgagcgcc tccgactcgg ggtcggcgta ggcggtggcc
     1261 tcgtctagca cgatgacgtc cgggttcgtc agcaggagcc tggcgatggc caggcgctgc
     1321 gtctcccctc ccgagagccg gaaggcgccg ccgaggcggg tggcgaggcc gtcgggcaag
     1381 gccgcgacga cccccgccaa ctgcgcggcg cccaggaccc tggccagttc ggcgtccgtg
     1441 gcgtcgagcc gcgcgaggcg gaggttggcg gccacggtgt cgtcgagcat ctggggctgt
     1501 tggaacacga aggccaggcg tctgccgagc tcggcggccg ggtagtcggc gatggggcgt
     1561 ccgtcaagcg tgacgacgcc gccctgcggg tcgtggagcc tggctgcgag ggcggccacg
     1621 gtcgacttgc ccgcccccga ccggccgacc agcgcggtga cggtccccgg ctcgaggtcg
     1681 aggctgaggt cgcgtacgac cggcctgcgt gggtggtagc cgaacgttac ggactcgaag
     1741 cgcagtcggc cgctccgtaa cggcagggga gccgtcgagg cgggcggcgc cagcggcggc
     1801 tcctcgagca gcagtccgat gcgccgcgcc gcggcgttcg cctcgcggac ggcggcggcc
     1861 gtgtagagga gaccgaggat gctcgtcccg aaggccgggg cgagcacgag gaacggcacg
     1921 agtcgcccga ggccgagtgc gccggccatc acgaggggca cgccgaccag gacgtacatg
     1981 ccgaacaacg tcgctggcct cacgacgatg tccgtgaggg ctcgcaggcg ggccgtcggg
     2041 cgctggaggc gctggaagaa cgtttggcgc tcctcgagca ggtcggcgaa cgccggtgta
     2101 gggaagaggc ggtccacctg gat
//
LOCUS       DAOQYE010000348         2120 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_60087_length_2120_cov_2.523971, whole genome shotgun sequence.
ACCESSION   DAOQYE010000348 DAOQYE010000000
VERSION     DAOQYE010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60087_length_2120_cov_2.523971"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1260)
                     /locus_tag="PKN52_06360"
     CDS             complement(<1..1260)
                     /locus_tag="PKN52_06360"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNQ99604.1"
                     /translation="MALSLVLASFTLAFAQPNDDLTVLQQDPGQWLMPNRTYSGWNYS
                     PLDQITKYNVKNLQVAWTFQIGVTDQIESAPLVVGTTMYILTPKPNTLYALDLNQQGY
                     IKWSFAPEMPGLAEAKACCGANSRGFMYADGKIIFNTLDGQLFALDANTGAVIWTTQV
                     ADLSLTETTTMSPLVAGHNVIVGVEGGERGIRGHVSAYDLDTGAQVWKFYNMGPDEEM
                     GIGPDFKPFYPTPDSPGTSTWYGDSWKTGGGAVWGWFTFDPESNLFYYGTGNCAPWNP
                     DYRRDPATAPGLDQYTNAYCASEMARDATTGNLVWAYQATPQDSWDYDLPAGQQLVDL
                     TIDGQTRRALMKPARNGFFYVWDAHTGELLHQPIKYTTVTWADHVDMETGRPVVNPDA
                     VVYTGTETGVVCPFGIGNWHNTTYSPKT"
     gene            complement(1403..>2120)
                     /locus_tag="PKN52_06365"
     CDS             complement(1403..>2120)
                     /locus_tag="PKN52_06365"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNQ99605.1"
                     /translation="LLLVVCAAQATTGATIQTATDATYGLHLTDADGNSLYLYTQDTP
                     QASACVDACATNWPAFTTDGDPVAGAGVDASLLGTLTRADGSVQVTYAGAPLYRYVRD
                     AKPGAINGQRLGGVFFLVSPQGKAIQDAVAQAAPALSDAALAALMSEGQQTFTANCAV
                     CHGDQGQGKVGPAFDKNANLGNTNYVIDTILGGIPPHGMPAWGGVLTDEQIASVATFI
                     RNSWSNAYGPVTQDAVTAQR"
BASE COUNT          285 a          678 c          775 g          382 t
ORIGIN      
        1 cgtcttgggc gagtaggtcg tgttgtgcca gttgccgatg ccgaaggggc agacgacgcc
       61 cgtctcggtg cccgtgtaga cgaccgcgtc cgggttgacg accgggcggc ccgtctccat
      121 gtcgacgtgg tcggcccagg tcaccgtcgt gtacttgatc ggctggtgca ggagctcgcc
      181 ggtgtgcgcg tcccacacgt agaagaagcc gttacgggcg ggcttcatga gggcccggcg
      241 cgtctggccg tcgatcgtca ggtcgacgag ctgctggccg gctggcaggt cgtagtccca
      301 cgagtcctgg ggcgtcgcct ggtaggccca gacgaggttg cccgtcgtgg cgtcgcgcgc
      361 catctcggag gcgcagtaag cgttcgtgta ctggtcgagg ccgggggccg tggcggggtc
      421 gcgccggtag tcggggttcc acggcgcgca gttgcccgtg ccgtaataga acaggttgga
      481 ctcggggtcg aaggtgaacc agccccagac ggcgccgccg cccgtcttcc aggagtcgcc
      541 gtaccaggtc gaggtcccgg ggctgtccgg cgtcgggtag aacggcttga agtcggggcc
      601 gatgcccatc tcctcgtcgg ggcccatgtt gtagaacttc cacacctgcg cgcccgtgtc
      661 gaggtcgtag gcggatacgt gcccgcggat cccgcgctcg ccgccttcca cgcccacgat
      721 cacgttgtgg ccggccacga ggggcgacat ggtcgtcgtc tcggtcaggc tgaggtcggc
      781 gacctgggtc gtccagatga cggcgcccgt gttcgcgtcg agcgcgaaca gctgaccgtc
      841 gagggtgttg aagatgatct tgccgtccgc gtacatgaac ccgcggctgt tcgcgccgca
      901 gcaggccttc gcctcggcca gcccgggcat ctcgggggcg aacgaccact tgatgtagcc
      961 ctgctggttg aggtcgagcg cgtagagcgt gttcggcttc ggggtcagga tgtacatggt
     1021 cgtgcccacg acgagcgggg ccgactcgat ctggtcggtg acgccgatct ggaacgtcca
     1081 ggcgacctgc aggttcttca cgttgtactt ggtgatctgg tcgagcgggc tgtagttcca
     1141 gcccgagtac gtgcggttgg gcatcagcca ctgcccgggg tcctgctgca ggaccgtgag
     1201 atcgtcgttc ggctgggcga aggccagggt gaacgacgcg agcacgaggc tcagggccaa
     1261 ggcggcccgg tggagccgag cgcgtcctgc gggttgttct cgcttgcctg acatcacaat
     1321 gcaccctcca agaggtcttg cggtttccgt atgcacgccc ccacgctccg gcggcggcgg
     1381 cgactgggta tggctccctc cttcacctct gcgcggtcac ggcgtcctgc gtgaccgggc
     1441 cgtacgcgtt ggaccacgag ttgcggatga acgtcgcgac ggaagcgatc tgctcgtcgg
     1501 tcagcacgcc tccccacgcg ggcatgccgt ggggcgggat gccgcccagg atggtgtcga
     1561 tcacgtagtt cgtgttgccg aggtttgcgt tcttgtcgaa ggccgggccg accttgcctt
     1621 ggccctggtc cccgtggcat acggcgcagt tcgccgtgaa ggtctgttga ccctcgctca
     1681 tcagggccgc gagcgcggcg tcgctcaggg ccggggccgc ctgggcgacc gcgtcctgaa
     1741 tcgccttgcc ttgcggagag acgaggaaga acacgcctcc caggcgctgg ccgttgatgg
     1801 cgcccggctt cgcgtcgcgc acgtagcggt acagcggcgc ccccgcgtac gtcacctgga
     1861 cggagccgtc ggcacgggtc agggtgccga gcagggacgc gtcgacgccg gcgccggcca
     1921 ccgggtcgcc gtcggtcgtg aacgccggcc agttcgtggc gcaggcgtcc acgcacgcgc
     1981 tcgcctgcgg cgtgtcctgc gtgtagaggt aaaggctgtt cccatcggca tccgtgaggt
     2041 gaagcccgta ggtggcgtcc gtcgccgtct ggatcgtcgc tcccgtcgtc gcctgagcgg
     2101 cgcacaccac caagaggagg
//
LOCUS       DAOQYE010000349         2119 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_60145_length_2119_cov_2.601260, whole genome shotgun sequence.
ACCESSION   DAOQYE010000349 DAOQYE010000000
VERSION     DAOQYE010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60145_length_2119_cov_2.601260"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1007
                     /locus_tag="PKN52_06370"
     CDS             <1..1007
                     /locus_tag="PKN52_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99606.1"
                     /translation="TVSPTQIVAVDPDVCRRCVHKPCLHGRVSAESEPASATAAAAAA
                     AVRLAPSRTAFIPMSEIVAPNGAGYFPGCESDLLVPTITSNLDCTYCFNCVRACPYDN
                     VALSVRAPGRELAKDPWRKRFGTSAIALGVLLTFWGLVNALAMISPFFAFTERLSEVL
                     GTRNEVVILGLAYLGVTVAGLALAVGAAWLADRLGGKRSSALTAFRRWGYVTVALGFG
                     FWGAHYIFHFLTGALSVVPVFEHFFEYRGFALDPNWRLAQVVPTRWLFPVQALLGAVY
                     AGLGLVMAARIGVRDFGLRRGVLAAWPVALYVLAFLALQLIILAQPMEMRGTLSGPLP
                     "
     gene            1115..2065
                     /locus_tag="PKN52_06375"
     CDS             1115..2065
                     /locus_tag="PKN52_06375"
                     /inference="COORDINATES: protein motif:HMM:NF014600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HNQ99607.1"
                     /translation="MLALVVVWTAVASGSSDHAADGSATELLASSTVGSPTGAPAGPP
                     GDALGSTMNSAVKGAVNGMTDPERTVANPPTTGDPQVSTDTQSSTDTQVSASAPLPDD
                     QLACVYADDPAPRAELAEWQHTVLDTRFKLPPDYAPDDLVELAAALADVAPGAAPAGI
                     QLRAVVLSDLRALLSAADAAGMKLAIQSAYRSYSYQESTFAYWVKQDGYEQALRSSAR
                     PGHSEHQLGTAMDFRSLTGPEAWDLDDWATTPEGAWTSENAWRFGFVMSYPAGAEGVT
                     CYMYEPWHYRYVGRELAAAVRASGLTLREYLLAHLVPDAR"
BASE COUNT          281 a          806 c          710 g          322 t
ORIGIN      
        1 gcacggtctc gcccacccag atcgtggccg tcgaccccga cgtctgccgg cggtgtgtcc
       61 acaagccgtg cctgcacggc cgggtcagcg ccgagagcga gcccgcatcc gcgaccgcgg
      121 cggcggccgc cgccgcggtc cggttggcgc cgtcgcggac cgcgttcatc cccatgagcg
      181 agatcgtggc gccgaacgga gccggctact tccccggctg cgagagcgac ctcctggtgc
      241 ccaccatcac ctcgaacctg gactgcacct actgcttcaa ctgcgtgcgc gcatgcccgt
      301 acgacaacgt ggcgttgagc gtgagggcgc caggacgcga gctggccaag gacccatggc
      361 gcaagcgctt cgggacgagc gctatcgcgc tcggcgtgct gctcacgttc tggggcctgg
      421 tgaacgctct ggccatgatc tcgcccttct tcgccttcac ggagcgactg tcggaggtgc
      481 tcggcacgcg caacgaggtg gtgatcctcg gcctcgcgta cctgggcgtc accgtcgccg
      541 gcttggcgct tgcggtggga gcggcatggc tggcggaccg cctgggcggg aagcgctcgt
      601 ccgccctcac ggcgttccgg cgctggggtt acgtcacggt cgcgctcggc ttcggcttct
      661 ggggcgcgca ctacatcttc catttcttga ccggcgcgct tagcgtggtg cctgtgttcg
      721 agcacttctt cgagtatcga ggcttcgcgc tcgaccccaa ctggcgcctc gcgcaggtcg
      781 tccccaccag atggctcttc cccgttcagg ccctgctcgg cgccgtctac gccggcctcg
      841 gtctggtcat ggccgcgcgc atcggcgtgc gcgatttcgg gctgagacgc ggtgtgctcg
      901 cggcgtggcc ggtcgcgctc tacgtcctcg ccttcctggc cctccagctc atcatcctcg
      961 cgcagcccat ggagatgcgc ggaacactct ccggaccgct cccgtgagcc agccacaccc
     1021 ccaaccgcga cggcccaggt gggcgcccga acgccggaac gcggaccgcc ccacccgccg
     1081 ccgccgcgcg cttcggggtc tcgccgtact ggtcgtgctg gcgctcgtgg tcgtctggac
     1141 ggcggtcgcg tcgggctcat ccgaccatgc ggccgacggc tccgccaccg agctcctcgc
     1201 cagcagtacc gtaggcagcc ctacaggcgc tcccgccggc ccccccggcg acgcgctagg
     1261 cagcactatg aacagcgcag tgaaaggcgc ggtgaacggc atgactgacc ccgaacggac
     1321 ggtcgcgaac ccgccgacca cgggcgaccc gcaggtgtcg accgacacgc agtcgtcgac
     1381 cgatacgcag gtgtcggcat cggcgcccct gccggacgac caactggcgt gcgtgtacgc
     1441 cgacgacccc gcgccgcggg cggagcttgc cgagtggcag cacacggtcc tcgacacgcg
     1501 cttcaagctc cctcccgact acgccccaga cgacctcgtc gagctcgccg cggcgctggc
     1561 ggacgtcgcc cccggcgccg ccccggcggg catccagctc cgggcggtcg tgctgagcga
     1621 cctgcgcgcc ctgctgagcg ccgccgacgc ggccggcatg aagctcgcca tccagtcggc
     1681 ctaccgctcc tactcctacc aggagtcgac cttcgcctac tgggtcaaac aggacggcta
     1741 cgagcaggcg ctgcgctcga gcgcccgtcc cggccactcg gagcaccagc tcggcaccgc
     1801 catggacttc cggagcctca ccggtccgga ggcctgggac ctggacgact gggcgaccac
     1861 cccggagggc gcctggacct cagagaacgc ctggcgcttc ggcttcgtca tgagctaccc
     1921 ggccggcgcg gagggcgtga cctgctacat gtacgagccg tggcactacc gttacgtcgg
     1981 gcgcgagctc gcggccgccg tgcgcgcgtc cggcctcacc ttgcgcgagt acctgctggc
     2041 ccacctggtg ccggatgcgc gctaaggcac gggtgtcgac ggagccccag cgcacgagcg
     2101 gcttcgcgcg cgtggcgac
//
LOCUS       DAOQYE010000350         2115 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_60281_length_2115_cov_3.524757, whole genome shotgun sequence.
ACCESSION   DAOQYE010000350 DAOQYE010000000
VERSION     DAOQYE010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60281_length_2115_cov_3.524757"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="PKN52_06380"
     CDS             complement(<1..259)
                     /locus_tag="PKN52_06380"
                     /inference="COORDINATES: protein motif:HMM:NF015735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HNQ99608.1"
                     /translation="MLYLVVAHDGTDAGAKERRAAARPSHLEGAKARFDAGGMPIGGA
                     ILDAAGAMIGSAMVVEAADEAEARAIVENDPYTKQGVWVRYD"
     gene            578..>2115
                     /locus_tag="PKN52_06385"
     CDS             578..>2115
                     /locus_tag="PKN52_06385"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNQ99609.1"
                     /translation="MPHRRLLLIAAAVLALIGSASAAGTLNYPMKADPEHLDAWRSST
                     TATRQILVNVLEGLTTFDGNSGDVIPALAESWDISDDGLTYTFHLRHGVLFHATPGVN
                     YADREVKADDVLWSLQRFLTEDTSISEHPEYVSSVSGSDAVLAGETDEWAGFKVIDDY
                     TVSMTLDAPNHRFLADLVNAYVVPKEALDALGSDLSNKPVGTGAFMFDRWTRDDQLVL
                     KANPDYWDGRPSLDGVTFWNVPDDTTGLLMYRQGELDVLVSFPDGQLESIKNEFAADY
                     HEGAGLNVAYWGFKMTEPPFADNVKLRQAFNYAVDRNLIWDNLMEGSRVPGNMGVLPP
                     DMPAADVEGYAYDLEKAKELLAEAGYPNGQGLEPITLFYYASSSDAPNIAVQDMLAQI
                     GVQINLQKEDNSTYWSHVGEPDVKLFLSGWSSDFLDPSEVFDFLFAHGRDDTKYDVPE
                     VNDLLDAARAESDPVAREAIYRQAHERIMADAPWIVSGYSKVSYLVKPNIENFQVSAA
                     GTYRS"
BASE COUNT          367 a          778 c          632 g          338 t
ORIGIN      
        1 cgtcgtacct gacccagacg ccctgcttcg tgtacgggtc gttctcgacg atggcacggg
       61 cctcggcctc gtcggccgcc tctacgacca tggcggagcc gatcatcgcg ccggcggcgt
      121 cgaggatggc gccgccgatc ggcatgccgc cggcgtcgaa gcgcgccttg gcgccctcca
      181 gatgcgacgg acgggcggca gcgcggcgct ccttggcgcc ggcgtccgtg ccgtcgtgag
      241 ccactacgag gtaaagcatg ctggacctcc ttggccgcga cctgccgacg ccgctttacg
      301 cgagcttggg tacccaggta ctcgaggccg atcgcgagcc aggataccgc gagcgcgaag
      361 ctcgcgtcct ggcgaaggtc cgttaacgga tgcgaccttt accgcgttca tgcactttgt
      421 tacacttcgc caacttcgtc cgtctcttgc cggcgtcttg aacgtctgtg aggtcgctca
      481 agtccgttgc ccccgcaagc agcgccccgg cggcctggtc tgccgggacg gaacgaaggg
      541 gtgcccgcgc caaaccggac acctgggagg agaagctgtg ccacatcgcc gacttctgct
      601 gatagccgcc gccgtcctcg ctctgatcgg gagcgcctcg gccgccggaa cgctcaacta
      661 cccgatgaaa gccgacccgg agcacctcga cgcgtggcgg tcgtccacga ccgccacgcg
      721 ccagatcctc gtcaacgtcc tagagggcct caccacgttc gacggcaaca gcggcgacgt
      781 aatcccggcc ctcgccgagt cttgggacat ctccgacgac gggctgacct acacgttcca
      841 cctgcggcat ggcgtcctct tccatgccac gccgggcgtc aactacgccg accgcgaggt
      901 caaggccgat gacgtcttgt ggtcactcca gcgcttcctt acggaagaca cctcgatctc
      961 cgagcacccc gagtacgtct cctccgtgag cggctccgac gcggtgctgg caggcgagac
     1021 cgacgagtgg gccggcttca aggtcatcga cgactacacc gtgtcgatga cgctcgacgc
     1081 gccgaaccac cgtttcctcg ccgacctcgt caacgcgtac gtcgttccga aggaagcgct
     1141 cgacgcgctc ggctccgacc tgtccaacaa gcccgtcggc accggcgcgt tcatgttcga
     1201 ccgctggacg agggacgacc agctcgtcct caaggccaac cctgactact gggacggccg
     1261 cccgagcctg gatggcgtga ccttctggaa cgttccggac gacacgaccg gcctcctcat
     1321 gtaccgccag ggcgagctcg acgtcctcgt cagcttcccc gacggccagc tcgagtcgat
     1381 caagaacgag ttcgcggccg actaccacga gggcgccggc ctcaacgtcg cctactgggg
     1441 cttcaagatg acggagccgc cgttcgccga caacgtcaag ctgcgccaag ccttcaacta
     1501 cgccgtcgac cgcaacctca tctgggacaa cctgatggaa ggcagccgcg tgccgggcaa
     1561 catgggcgtc ctgccgcccg acatgcccgc cgccgacgtc gagggctacg cgtatgacct
     1621 cgagaaggcc aaggagctcc tcgccgaggc cggctacccg aacggccagg gcctcgagcc
     1681 gatcacgctc ttctactacg cctcctcctc tgacgcgccc aacatcgcgg tgcaggacat
     1741 gctggcccag atcggcgtgc agatcaacct gcagaaggaa gacaacagca cctactggtc
     1801 gcacgtcggc gagcccgacg tcaagctctt cctctccggc tggagctccg acttcctgga
     1861 cccgagcgag gtcttcgact tcctcttcgc gcatggccgc gacgacacca agtacgacgt
     1921 cccggaagtc aacgacctcc tcgacgccgc cagggccgag agcgaccccg tcgcccgcga
     1981 ggccatctac cgccaggcgc acgagcggat catggccgac gccccatgga tcgtctccgg
     2041 ctactccaag gtctcgtacc tggtcaagcc gaacatcgag aacttccagg tctccgcggc
     2101 cggcacctac cgttc
//
LOCUS       DAOQYE010000351         2113 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_60376_length_2113_cov_2.958212, whole genome shotgun sequence.
ACCESSION   DAOQYE010000351 DAOQYE010000000
VERSION     DAOQYE010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60376_length_2113_cov_2.958212"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            254..1087
                     /locus_tag="PKN52_06390"
     CDS             254..1087
                     /locus_tag="PKN52_06390"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNQ99610.1"
                     /translation="MDEEYAGDGRERGGVAPAVAPNGAPTGAPVDGRNAAPYTDLAGV
                     YDRLMADIEYDEWAAFIVRTAARRGLPSGSAGSGLLDLGCGTGNATLPMWELGYEVEG
                     LDASSAMLAAARKKLPGIVFHLDTFEEFSLGRRFQLVYSVFDALNNLLTEAAFARCLA
                     RVRDHLVPGGVFMFDVNTPTGLRELWLDGVSEGWADDVYYRWTHTYDEESGLATVEAY
                     CETGEGSFVEVHQERGYATPDLVRLLAAAGFADVAVIEYPDGQPARPDAERVWVVASS
                     P"
     gene            complement(1105..1980)
                     /locus_tag="PKN52_06395"
     CDS             complement(1105..1980)
                     /locus_tag="PKN52_06395"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNQ99611.1"
                     /translation="MRIGFIGLGTMGAPMAARLLAAGFEVSVHNRTREREEPLAALGA
                     KRAASPKSCAQDSDVVFTMVSDTPDVQAVVTGDHGAIEGLAEGSVLVDMSTISPATTR
                     RLAAALAQRGVQMRAAPVSGGSEGARNGTLSIMVGGDAVALERVRPVLEHLGRTITHV
                     GPVGSGQVAKAVNQVIIAGTYAAVAEGMALAMAAGIDTDAALTALGGGAAGSWVLANR
                     ARNMLAGDYPLGFRTRLHRKDLGIALASARELGVSMPVAAYVEQVETSLVARGFGDED
                     VSNVARVVREQAGLK"
BASE COUNT          281 a          739 c          759 g          334 t
ORIGIN      
        1 agcgtcttgt cgtgttcggt cgtcgtgtgg gggaggttgg tggttcgtcc ttgatcgtgt
       61 ctcggtcgtg cacgacttct gacgccgtgc cgtcaagagt gaacagaccc tagtggcgtc
      121 gcgccggcta cccggcttgg gctggccgaa gcggttcccg cccgacgcgc tcggcttagg
      181 cggccatgcg cgcggccgtt gaacgccacc ggccgaaccc cggtcggcct aggccggcgg
      241 ttatcatccg cgcgtggacg aggagtacgc gggcgacggt cgtgagcggg gcggagtcgc
      301 gccggccgtc gcgccaaatg gcgcgccaac tggcgcgccg gtcgacggtc gcaacgccgc
      361 cccctacacg gacctggccg gcgtctacga ccgcctcatg gcggacatcg agtacgacga
      421 gtgggccgcc ttcatcgtgc gcacggccgc acgccgcggc ttaccgagcg gttcggccgg
      481 gagcgggctc ctcgacctcg gatgcggcac cggcaacgcg acgctaccga tgtgggagct
      541 ggggtatgag gtcgagggtc tggacgccag ctccgccatg ctcgccgcgg cccgcaagaa
      601 gctgccaggc atcgtgttcc acctcgacac gttcgaggag ttctcgctcg ggcggcgctt
      661 ccagctcgtg tactccgtat tcgacgccct caacaacctg ctgaccgagg cggccttcgc
      721 ccgttgcctc gcccgcgtgc gcgaccacct cgtgcccggc ggcgtcttca tgttcgacgt
      781 gaacacgccc acggggttga gggagttatg gcttgacggc gtgtccgagg ggtgggccga
      841 cgacgtctac taccgctgga cgcatactta cgacgaggag agcgggctcg cgacggtgga
      901 ggcgtactgc gagacgggcg agggctcgtt cgtggaggtg caccaggagc gcggctacgc
      961 gacccccgac ctcgtgcggc tcctggcggc ggcgggcttc gcggacgtcg cggtcatcga
     1021 gtacccggac ggccaaccgg cgcggcccga cgccgaacgc gtctgggtgg tggccagctc
     1081 gccctgacgc cgccaagggt ctggctactt gagcccggcc tgctcgcgca ccacgcgggc
     1141 gacgttcgag acgtcctcgt cgccgaagcc gcgcgccact aggctggtct cgacctgctc
     1201 aacgtacgcc gccaccggca tgctgacgcc gagctcgcgc gccgatgcga gcgcgatgcc
     1261 caggtctttc ctatgcaggc gcgtgcggaa gccgagcggg tagtcgccgg cgagcatgtt
     1321 cctggcccgg ttggccagta cccaggagcc ggccgctccc ccgccgaggg cggtgagcgc
     1381 cgcgtcggtg tcgatcccgg ccgccatcgc caaggccatg ccctcggcga cggcggcgta
     1441 cgtaccagcg atgatgacct ggttgaccgc cttcgccacc tgtccgctgc cgaccgggcc
     1501 gacgtgggtg atggtgcggc ccaagtgctc cagcacgggg cgcacgcgct ccagggccac
     1561 ggcgtcgccg ccgaccatga tggatagcgt gccgttcctg gccccctcgc tgccgccgga
     1621 gaccggcgcg gcgcgcatct gaacgccgcg ctgggcgagt gcggcggcca gacgccgcgt
     1681 cgtggcgggg ctgatggtgc tcatgtcgac gagcaccgag ccttccgcca acccctcgat
     1741 ggcgccgtga tcgccggtga cgacggcctg cacgtccggc gtatcggaga ccatggtgaa
     1801 cacgacgtcg ctgtcctggg cgcagctctt cgggctcgct gcccgcttgg cgccgagggc
     1861 cgccagcggc tcctcgcgct cgcgcgtacg gttgtggacg ctcacctcga agcctgccgc
     1921 caggagcctg gcggccatcg gcgcgcccat ggtgccgagt ccgatgaagc cgatgcgcat
     1981 gtcgttgtcc tccaaagacg gtcgccctgg tcgagcggta ggcgctcgaa ggtctcagga
     2041 cgaggttacc gttccgacga gcccggtgtc ctgccagatg gtgaggagcg tctccgccgc
     2101 cctggtcgcg acg
//
LOCUS       DAOQYE010000352         2112 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_60431_length_2112_cov_2.786096, whole genome shotgun sequence.
ACCESSION   DAOQYE010000352 DAOQYE010000000
VERSION     DAOQYE010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60431_length_2112_cov_2.786096"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="PKN52_06400"
     CDS             complement(<1..367)
                     /locus_tag="PKN52_06400"
                     /inference="COORDINATES: protein motif:HMM:NF013116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HNQ99612.1"
                     /translation="MNPLLRLRQFGQTVYLDEFRRSWLTNGRLQALIDDDGLAGVTSN
                     PAIFQKAIVESHDYDEAIAELAKGGSTPYLAYEELTVADIRAAADLFRPMYDASNGVD
                     GFVSLEVSPHLANDEEGTVA"
     gene            complement(443..1126)
                     /locus_tag="PKN52_06405"
     CDS             complement(443..1126)
                     /locus_tag="PKN52_06405"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HNQ99613.1"
                     /translation="MNALGGAGDPSGKEPAGETPPAQAPVSDREREAPPETLSRRQLI
                     KWLWRVPVLAALIGGGYGVYEAINVHFLKRNPTAAPEFEPVADVAVGTVAAFANVWDE
                     LPFELPTGGPGGGPLPALAVRLPGPIPGGVAVDSGVGTASAYLAAFSRVCTHQHCIVV
                     LNRDVDAINFGFNYETDSPKLTCPCHLSVFDPMGAGRAVSGPAILPLPRVRLELRGAS
                     VVATGIERA"
     gene            complement(1132..>2112)
                     /locus_tag="PKN52_06410"
     CDS             complement(1132..>2112)
                     /locus_tag="PKN52_06410"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNQ99614.1"
                     /translation="EAEATYRRIVDEVSPVPVDAYKRLAILALQSDLAQAQGWLQLAS
                     GVQPDDAETLYLLGEVAFARGDLDRAAEAFSAFAATPDGTSEPTAQRRIALIERVKPL
                     QAAVLADPSEANLLALGDAYWEASEPQKAVESYFRVLTDYDSSQPRALSRTGQLLYVA
                     GRPSDAVGAIERAATAAGGVAELEPDAVMTLADAYSQTGALEDAARTYDLYAELVGPE
                     LGAPAKALAEAARAQASGAADGGGTDAGLTVAGRQVFSTYCVECHGQRGEGGIGAQLA
                     GNQRATSEANVRDAVRFGRGMMPAFQASLEPAQLDAVVAYVTQVLSRSGL"
BASE COUNT          271 a          720 c          794 g          327 t
ORIGIN      
        1 cggcgacggt gccctcctcg tcgttggcga ggtgggggga gacctcgagg gagacgaagc
       61 cgtccacgcc gttcgaggcg tcgtacatgg ggcggaagag gtcggccgcg gcgcggatgt
      121 cggcgaccgt cagctcctcg tacgccaggt agggggtgga gccgcccttg gccagctcgg
      181 cgatggcttc gtcgtagtcg tggctctcga cgatggcctt ctggaagatg gcggggttcg
      241 aggtcacgcc ggcgaggccg tcgtcgtcga tcagggcctg tagcctgccg ttcgtgagcc
      301 acgagcggcg gaactcgtcg agatagaccg tctgtccgaa ctgcctcagg cgcagtagtg
      361 ggttcatgtc cgtcctccgg gcgttgatgg gcgctgacct acttgctgcg tccaggcgcg
      421 ccgcctacgc agatgataag gctcaggccc gctcgatgcc cgtagccacc acgctcgcgc
      481 cgcgcaactc gagtcgcacg cggggcagtg gcaggatggc cggcccgctc acggcgcggc
      541 ctgcgcccat ggggtcgaag acgctcaggt gacatgggca ggtgagcttc gggctgtccg
      601 tctcgtagtt gaagccgaag ttgatggcgt cgacgtcgcg gttgaggacc acgatgcagt
      661 gttggtgcgt gcagacgcgg ctgaacgccg ctaggtaggc gctcgcggta cctaccccgc
      721 tgtcgaccgc cacgccgccc gggatcgggc ctggcaggcg caccgccagc gcgggcaggg
      781 ggccgccgcc cggcccgccc gtaggcagtt cgaacgggag ctcgtcccac acgttcgcga
      841 aggccgctac ggttccgacc gcgacgtcgg caacgggctc gaactccggc gcggccgtcg
      901 ggttgcgctt gaggaagtgg acgttgatgg cctcgtagac gccgtacccg ccgccgatga
      961 gcgcggcaag cacgggcacg cgccacagcc acttgatgag ctggcggcgg gagagcgtct
     1021 cgggaggcgc ttcgcgctcg cggtcgctga ccggcgcctg agcaggtggc gtctcgccgg
     1081 ccggttcctt gccggaaggg tccccagctc cgccaagcgc gttcacggcc gctagagccc
     1141 gctccgggag aggacctggg tcacgtacgc caccacggcg tccagctggg cgggctccag
     1201 gctcgcctgg aaggccggca tcatgccgcg gccgaagcgc acggcgtcgc gcacgttggc
     1261 ctcgctggtg gcccgctggt tgccggcgag ctgggcgccg atgcccccct cgccgcgctg
     1321 gccgtggcac tcgacgcagt aggtggagaa gacctgcctg cccgcgaccg tgaggccggc
     1381 atcggtgccg cctccatccg ctgcgccact cgcttgcgcg cgggcggcct cggccagcgc
     1441 tttcgctggc gcgccgagtt ccgggcccac gagctcggcg tagaggtcgt acgtccgggc
     1501 cgcgtcctcc aacgcgcccg tctgggagta ggcgtccgcg agcgtcatga ctgcgtcggg
     1561 ctccaactcg gccacgccgc cggccgcggt ggccgcgcgc tcgatggccc cgacggcgtc
     1621 gctcggtcgc cccgcgacgt agaggagctg acccgtgcgg gagagcgccc gcggctggct
     1681 cgagtcgtag tcggtcagca cccggaagta gctctccacg gccttctgcg gttcgctggc
     1741 ttcccagtag gcgtcgccga gcgccaggag gttcgcctcg gacgggtccg cgaggacggc
     1801 tgcctgtaga ggcttgacgc gttcgatgag cgcgatgcgg cgctgggcgg tcggctcgct
     1861 ggtcccgtcg ggtgtggcgg cgaaggcgct gaaggcctcc gcggcccggt cgaggtcgcc
     1921 gcgcgcgaac gcgacctcgc ccagcaggta gagcgtctcg gcgtcgtccg gttggacccc
     1981 gctggcgagc tggagccatc cctgggcctg cgcgaggtcg ctctgcaggg cgaggatggc
     2041 aaggcgctta taggcgtcga ccgggaccgg actcacctcg tccacgatgc gtcggtaggt
     2101 cgcctctgcc tc
//
LOCUS       DAOQYE010000353         2101 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_60948_length_2101_cov_1.938905, whole genome shotgun sequence.
ACCESSION   DAOQYE010000353 DAOQYE010000000
VERSION     DAOQYE010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60948_length_2101_cov_1.938905"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..788
                     /locus_tag="PKN52_06415"
     CDS             <1..788
                     /locus_tag="PKN52_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNQ99615.1"
                     /translation="INFTGSVATGVRIFERAAKVHPGQHWLKRTFLEMGGKDALIVDA
                     SADVELAASAAVASAFGFQGQKCSAMSRLIVVDDVYDAVLERVKALTEKLSVGPADEN
                     HDVAAVINERQYQKVLGYIETGKSEARLLTGGGAAGGDGWFIEPTVFADVPVGASIAC
                     DEIFGPVVSVLRARDFDHALEIANGSMYALTGGVISNDRRNLEKARREFQAGNLYLNR
                     KITGALVGVQPFGGFKMSGSNTKAGGPDYLRNFMEMKTVTERF"
     gene            complement(785..1465)
                     /locus_tag="PKN52_06420"
     CDS             complement(785..1465)
                     /locus_tag="PKN52_06420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99616.1"
                     /translation="MKRTLIALALAFAALASTGLAQEYSVVVDGVRSPIAAVTLNGVV
                     YVPLQALTDVGVVATFDGGALSLTLPPKAAGGAGQRESVEGCLRQDLFNGVWRVRVTD
                     VSPLTGDKPGWGVTVEVRNGSTTTLMPTDAGVDGTGEGIQLVLADGTILAADPYDVQE
                     LTFASLPQGGGLTHQLLFYLPSGASLGQPDKFLFEFHADGIGFSEQQAGVAFTVPNPS
                     LRVRLGCG"
     gene            complement(1613..>2101)
                     /locus_tag="PKN52_06425"
     CDS             complement(1613..>2101)
                     /locus_tag="PKN52_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HNQ99617.1"
                     /translation="STLLHAVRAGPTAERWLRRLDHGAIYVLIAGSYTPITLVALQPE
                     RATLGWWLFGLVWLCAAGGLLFKTFWLGAPRWLSTALYLAMGWLVVLAIVPVARSLGT
                     VNTVWLGLGGLFYSVGAVVYALKRPRLWPDVFGYHELWHLFVLAGWGCHLVMMVRLAA
                     PV"
BASE COUNT          355 a          709 c          749 g          288 t
ORIGIN      
        1 tcatcaactt caccggctcg gtggccaccg gcgtgcgcat cttcgaacgc gcggccaagg
       61 tgcacccggg tcagcattgg ctcaagcgca cgttcctcga gatgggcggc aaggacgcgc
      121 tcatcgtcga cgcctccgcc gatgtcgagc tggccgcctc cgccgccgtg gcgagcgcct
      181 tcggcttcca gggccagaag tgctcggcga tgagcaggct catcgtggtc gatgacgtgt
      241 acgacgccgt gttggagcgc gtcaaggcgc tcaccgagaa gttgagcgtc ggcccggccg
      301 acgagaacca cgacgtcgcc gccgtcatca acgagcggca gtaccagaag gtcctcggct
      361 acatcgagac gggtaagtcc gaggcgcgcc tgctcactgg cggcggtgcg gccgggggcg
      421 acggctggtt catcgagccg acggtgttcg ccgacgtgcc ggtcggcgcc agcatcgcgt
      481 gcgacgagat cttcggaccc gtcgtcagcg tgctgcgggc ccgcgacttc gaccacgcgc
      541 tcgagatcgc caacggcagc atgtacgctc tgaccggcgg cgtgatcagc aacgacaggc
      601 gcaacctcga gaaggcgcgc cgcgagttcc aggccggcaa cctctacctc aaccggaaga
      661 tcacgggcgc cctcgtcggc gtccagccgt tcggcggctt caagatgtcc ggctcgaaca
      721 ccaaggcggg cggaccggac tacctgcgca acttcatgga gatgaagacc gtcaccgagc
      781 ggttctagcc gcagcccagc ctcacccgca ggctcgggtt gggcaccgtg aaggccacgc
      841 ccgcctgttg ctcggagaac ccgatgccgt ccgcgtggaa ctcgaagagg aacttgtccg
      901 gctggccgag gctggcgccg ctcgggaggt agaagagcag ttggtgcgtc aggcccccgc
      961 cctgcggcag gctggcgaac gttaattcct gaacgtcgta cgggtccgcc gcgaggatgg
     1021 tgccgtccgc caagacgagt tggatgccct cccccgtgcc gtccacgccg gcgtccgtcg
     1081 gcatgagggt cgtggtggag ccgttgcgca cctcgacggt gacgccccag ccaggcttgt
     1141 cgcccgtcaa cggtgaaacg tcggtcaccc gcacgcgcca gacgccgttg aagaggtcct
     1201 gccgcaggca gccttcgacc gactcgcgct ggccggcgcc gccggccgcc ttcggcggca
     1261 gggtcaagga gagcgctccc ccgtcgaagg tcgccaccac gcccacatcg gtgagggcct
     1321 gaagcggcac gtagacgacg ccgttcaggg tcactgccgc gatgggcgac cgcaccccgt
     1381 caacgacgac cgagtactcc tgtgcgaggc cggtgctcgc gagggccgcg aaggccaacg
     1441 ctagcgcgat gagggtccgt ttcatggtgc acctccgggg cgcggtcgct ggcgggggcg
     1501 cgctacgcgc gcccgaacgg ccgagcctgg catgcgcggc agcctagggc ggggcgggtt
     1561 atacgcgggt tatacgtggg ttatacgggt gttacacgga gtgtggcgcg ggtcagaccg
     1621 gtgcggcgag gcgcaccatc atcacgaggt ggcagcccca acccgcgagc acgaacaggt
     1681 gccagagctc gtggtagccg aacacgtccg gccacaggcg cggacgcttg agggcgtaga
     1741 cgaccgcccc gacgctgtag aagaggccgc ccagccccag ccagacggtg ttgaccgttc
     1801 cgagcgacct ggcgaccggc acgatggcga ggacgacgag ccagcccatg gccaggtaga
     1861 gcgccgtgct gagccagcgc ggcgcgccca accagaacgt cttgaagagc aggccgccgg
     1921 cggcgcagag ccagacgagc ccgaagagcc accaccccaa ggtggcgcgc tccggctgta
     1981 gcgccacgag cgttatgggg gtgtaggagc cggcgatcag gacgtagatg gcgccatgat
     2041 cgagccgcct gagccaccgc tcggccgtgg ggccggcgcg cacggcgtgc aggagggtgc
     2101 t
//
LOCUS       DAOQYE010000354         2099 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_61000_length_2099_cov_3.537671, whole genome shotgun sequence.
ACCESSION   DAOQYE010000354 DAOQYE010000000
VERSION     DAOQYE010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61000_length_2099_cov_3.537671"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            364..831
                     /locus_tag="PKN52_06430"
     CDS             364..831
                     /locus_tag="PKN52_06430"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HNQ99618.1"
                     /translation="MVEKLARLKRTRLITALDDVDPAEDLRRGGSPHWPGYVATVLRG
                     EVRWADLSRGRGSEEDSGSEQDGHRPVLVISHDVFNERSSTVIAVALTSREPRAGFPL
                     TLESHASGLPRRSWVKVSQIRTLAVERMGALIGKATPEEVDLVVEGLNEIVGG"
     gene            complement(900..1841)
                     /locus_tag="PKN52_06435"
     CDS             complement(900..1841)
                     /locus_tag="PKN52_06435"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNQ99619.1"
                     /translation="MSGKGTRLKQVIIDRIRSGDYPVGHRLTSARTAANEFGVHSNTV
                     SRIYKELADDGIVRTVHGSGTFVVSVPGPEHGVGAVDELSSSLAALAEQARYLGLSRG
                     AWDELVAESSSQAFLGSEPGIWMVECSRKDVEELSLRLSTLLRRSVNPLLVDEVPGRL
                     RTCGPGDIFLTTPFHYEEVSAMLEAERSLLNVNVVPTTDTLVAFAQLEPGADINIVSS
                     NRPTLERLVRMVKTYAKATTGCATLIDAPEAAAVVRSARVLVDTQSIHERVMGWRPTG
                     HVLTVRYQIEPTSVAYVREVLRIREAEPGHGELVGQL"
     gene            complement(1879..>2099)
                     /locus_tag="PKN52_06440"
     CDS             complement(1879..>2099)
                     /locus_tag="PKN52_06440"
                     /inference="COORDINATES: protein motif:HMM:NF014063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033735 - aspartate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DUF108 domain-containing protein"
                     /protein_id="HNQ99620.1"
                     /translation="VAVALSLAGIGPDLTQVVVRSDPRAEGVMQLIEASGPLAALEVK
                     IRSQPSPQNPRTSYMAGAAAVSAVRAIG"
BASE COUNT          350 a          648 c          733 g          368 t
ORIGIN      
        1 tgcctcgttg catatcgtgt cgtttttcaa actagacctc ccatgctcgc cgtgctggct
       61 tgggcggtag cgtcttggaa ggtagccgtg ttgtatccgc tgcgctgcac ggttagaacc
      121 caagcagggt gcgcaaacgg ttcttgagtc gtctaggggc gcgtgcccgt agagtgtttt
      181 gttgagttcg gtcgtcgtgt gggggaggtt ggtggttcgt ccctgatcgt gtctcggtcg
      241 tgcatgacgt ctaacgttgt gccgtcaaga gtgaatagaa cctagatcag gcgtacttgg
      301 ttgtggccta gctcagcgaa ccctaggccg ggatctcgcg caaggctggc catcgaggcg
      361 gccgtggtcg agaagctggc gagactcaag cgcacgaggc tcatcacggc actcgacgat
      421 gtcgaccccg ccgaagacct tcgcagagga ggatctccac actggcctgg ctatgtggcc
      481 acggtactga ggggcgaagt ccgttgggcc gacctgagtc gtggccgcgg cagcgaagag
      541 gatagcggca gcgaacagga tgggcatagg cccgtactgg tcatcagcca tgacgtgttc
      601 aacgagcgct ctagcacggt gatagccgtc gcgcttacca gccgggaacc cagagccggg
      661 ttcccgctca ccctggagtc gcacgcctcg gggttgccgc ggcgttcgtg ggtgaaggtg
      721 agccagatcc gcactctcgc ggtggagcgg atgggcgcgc tcatcggcaa ggcgacgccg
      781 gaagaggtcg acttggtcgt ggaggggctg aacgagatcg tgggagggtg aggccgggcg
      841 agcgcagccg cgatgaagtg gcccagttcg aacgctacta gggccgcttg gcgcccaggc
      901 tagagctgtc cgacgagctc cccgtgccca ggctccgcct cgcgtatgcg cagcacttcc
      961 ctgacgtaag cgaccgaggt cggttcgatc tggtagcgga cggtcagcac gtggccggta
     1021 ggccgccagc ccatcacgcg ctcgtggatc gactgcgtgt cgacgagcac ccgcgccgag
     1081 cggacgacgg cggccgcctc gggcgcgtcg atgagggtgg cgcagcctgt ggtggccttg
     1141 gcgtacgtct tcaccatgcg cacaaggcgc tcgagcgtcg ggcggttgga cgacacgatg
     1201 ttgatgtcgg cgccgggttc cagctgggcg aaggccacca gcgtatccgt cgtcggcacc
     1261 acgttgacgt tgaggagcga ccgctccgct tccaacatgg cggagacctc ctcgtagtgg
     1321 aacggcgtgg tcaggaagat gtcgccaggg ccgcaggtgc ggagccgacc agggacctcg
     1381 tcgacgagca gcgggttgac gctgcggcgc agtagcgtcg acagtcggag ggacaactcc
     1441 tcgacgtcct tcctgctgca ctccaccatc cagatgcccg gctcggagcc gaggaacgct
     1501 tgggacgagc tctcggccac gagctcgtcc caagccccgc gcgacaatcc caggtaacgg
     1561 gcctgctcgg ccagcgcggc gagggaggaa gacagctcgt ccaccgcccc tacgccgtgc
     1621 tccggcccgg gcaccgagac cacgaaggtg ccgctgccgt ggacggtccg cacgatgccg
     1681 tcgtccgcca gctccttgta gatgcggctg acggtgttcg aatgcacgcc gaactcgttg
     1741 gcggccgtac gcgcgctggt caaccggtgg ccaacggggt agtcgcccga ccggatgcgg
     1801 tcgatgatca cctgcttcaa acgtgtacct ttgccagaca tcttctagtg tccaccgctc
     1861 atgaggtttg ccactagact acccaatcgc gcgcacggca gatacggcgg ccgcgcccgc
     1921 catgtaagac gtgcgggggt tctgggggct cggctgcgac cggatcttca cctccagtgc
     1981 ggccaagggt ccggaggcct ctatcaactg catgacgcct tccgcgcgcg ggtcggagcg
     2041 cacgaccacc tgggtcaggt cgggtccgat gccggccagg ctgagcgcga cggcgacgt
//
LOCUS       DAOQYE010000355         2096 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_61124_length_2096_cov_3.858893, whole genome shotgun sequence.
ACCESSION   DAOQYE010000355 DAOQYE010000000
VERSION     DAOQYE010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61124_length_2096_cov_3.858893"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..593
                     /locus_tag="PKN52_06445"
     CDS             <1..593
                     /locus_tag="PKN52_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99621.1"
                     /translation="SGFGDPFLEILDTQTNEFVYSGLEGVVHSNLVWTKDLELLFTME
                     FGAQATEEVFAGVVAVPLEEFQERGDTLELKVLEVFNQADWGLGGVYDLALSADETQL
                     AYVRNGDIWVKDLTTTASAKQLTTGPTPIAGPAFSPDSSLIVFVGVRPYGLNDTLVLP
                     NDGTGPYVVNANDRSVTQAYVLDERNLVDVVMVWLP"
     gene            complement(731..1219)
                     /locus_tag="PKN52_06450"
     CDS             complement(731..1219)
                     /locus_tag="PKN52_06450"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HNQ99622.1"
                     /translation="MTPQGNNYKHTSAIIDDRTIRVERVFDAPRDLVWRAYSEPELVA
                     QWWGRGNKVDIEKFEFHRGGHWRFVEHHEGGSDGFEGRFREIEPKTLISQTFEWDGMP
                     GHPIIDTVEFSDANGGGATKISATSHFFNQEERDGMAAAGAEAGMAQSYAALDSLLER
                     MK"
     gene            complement(1216..1557)
                     /locus_tag="PKN52_06455"
     CDS             complement(1216..1557)
                     /locus_tag="PKN52_06455"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNQ99623.1"
                     /translation="MVQYRAQAPIHETFAALADPTRIAFLERLERADATITQLAERAG
                     ISLPGARKHVRVLEGVGLVRTSKQGRARVCTLGPRRLEREAAWLEGYRRTLEERFDHL
                     GKLLDRLKEES"
     gene            1807..>2096
                     /locus_tag="PKN52_06460"
     CDS             1807..>2096
                     /locus_tag="PKN52_06460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99624.1"
                     /translation="MAYAFGFGHGAGLGFGLGFLNLVGTLLFFLFIVFLIKTLARGGW
                     RGGWGGRRGERPDDERWGFRWGPDARNRMEEPLGPGHSRYGHDVPGHDQAWQT"
BASE COUNT          358 a          686 c          673 g          379 t
ORIGIN      
        1 tatcgggctt cggcgatccc ttcctcgaga tcctcgacac gcagacgaac gagttcgtct
       61 attcgggact cgaaggcgtc gtgcacagca acctcgtctg gaccaaggac ctcgagctgc
      121 ttttcaccat ggagttcgga gcgcaagcca ccgaggaggt gttcgcgggc gtggtcgctg
      181 ttccgttgga ggagttccag gagcgcgggg acaccctgga gctcaaggtc ctggaggtct
      241 tcaaccaagc cgactggggc cttggagggg tgtacgacct ggcgctctcg gccgacgaga
      301 cccagctggc gtacgttcgc aacggcgaca tctgggtcaa ggacctgacc accaccgcgt
      361 ccgcgaagca actgaccacg ggtccgactc cgattgcggg tccggctttc tcgccggaca
      421 gctcgttgat cgtgttcgtc ggcgtacgac cctacggtct gaacgacacg ctggtgctgc
      481 cgaacgacgg aacggggcct tacgtcgtga acgccaacga ccgttccgtg acccaggcat
      541 acgtgctgga cgagaggaac ctcgtggatg ttgtcatggt ctggttgcct tagctaccga
      601 ccaggcggag ccggcgcggt ctggtcaagg tcgaagctct ccaacgagcg agcccccttc
      661 tagcgcggcc gaagagggct caccgtagtt ttcgaatagc gacggcgcct actccagctg
      721 ccgcaacggc ctacttcatc cgctccagca aggaatccag cgccgcgtag ctctgggcca
      781 tgccggcctc ggcaccggcc gcggccatgc cgtcgcgctc ctcttggttg aagaagtgcg
      841 acgtggccga gatcttcgtg gcgccgccgc cgttagcgtc gctgaactct accgtgtcga
      901 tgatcgggtg accgggcatg ccatcccact cgaaggtctg ggagatgagc gtcttcggct
      961 cgatctcgcg gaagcgcccc tcgaagccgt cggagccgcc ctcgtggtgc tcgacgaagc
     1021 gccagtggcc gcccctatgg aactcgaact tctcgatgtc gaccttgttg ccgcggcccc
     1081 accactgcgc gacgagttcg ggttcggagt aggcgcgcca cacgaggtcg cgcggggcgt
     1141 cgaagacgcg ctccacacgg atggttcggt cgtcgatgat cgcggacgtg tgcttgtagt
     1201 tgtttccttg cggggtcatg actcctcctt caggcggtcc aacagcttgc cgagatggtc
     1261 gaagcgctct tcgagcgtgc gccggtaacc ctcgagccac gcggcctcgc gctccagccg
     1321 gcgcggcccc aacgtgcaga cccgcgccct accctgcttg ctggtgcgga cgagaccgac
     1381 gccctccagc accctgacgt gcttccttgc gccaggcagc gagatgcccg cccgctcggc
     1441 gagctgggtg atagtcgcgt cggcacgctc caaccgctcc aagaacgcga tgcgggtcgg
     1501 gtccgccaag gcggcgaacg tctcgtggat aggagcttga gcccgatact gaaccatctg
     1561 gttcagtatc ttggtcgtgg ggtgagttgt caagggggac gccacctcga tacaaacaga
     1621 gtccgttggt cgtatctcct acagcaccaa gccctcggct tgcgcggcgg ccaagccgac
     1681 cacacaaaca ccccctccca ttcacatatc gttcacaccg cacccccctg accttcacga
     1741 cccccgccct aacctcgctc cagaccaggg gccaaacggc ccgactggcg aaaggagcga
     1801 ttctctatgg catacgcatt cggattcggc cacggggccg gcttggggtt cggactgggg
     1861 ttcttgaacc tcgtgggtac cctactgttc ttcctcttca tcgtgttcct gatcaagaca
     1921 ctggcgcgcg gtggttggcg gggcggatgg ggcggcaggc gcggcgagcg tcccgacgac
     1981 gagcgctggg ggttcaggtg ggggcccgac gccaggaacc gcatggaaga gccgctcggg
     2041 ccggggcaca gtaggtatgg gcacgacgta cctggacacg accaagcctg gcagac
//
LOCUS       DAOQYE010000356         2094 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_61245_length_2094_cov_2.480628, whole genome shotgun sequence.
ACCESSION   DAOQYE010000356 DAOQYE010000000
VERSION     DAOQYE010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61245_length_2094_cov_2.480628"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..762
                     /locus_tag="PKN52_06465"
     CDS             <1..762
                     /locus_tag="PKN52_06465"
                     /inference="COORDINATES: protein motif:HMM:NF013895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HNQ99625.1"
                     /translation="LRATDDQETAVAVFRKHDRSALPVVDSSGVIVGIVTVDDVLDVA
                     EEEATEDIQMLGGVEALEEPYLSISLARVIRKRAFWLVLLFLGQTLTATAMGFFEAEI
                     ASAVVLALFLPLIISSGGNSGSQAAALVTRAMALGEIGVKDWWAIAKREVLAGLALGV
                     LLGGLGFARVAVWGVVLRAYGDDWFLIGVTVFIALIGVVLWGSLTGSLLPIVLKRLGA
                     DPAASSTPFVATIVDVVGILIFFGLAKLVLTGTLL"
     gene            1177..1341
                     /gene="lysW"
                     /locus_tag="PKN52_06470"
     CDS             1177..1341
                     /gene="lysW"
                     /locus_tag="PKN52_06470"
                     /inference="COORDINATES: protein motif:HMM:TIGR01206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019878 - lysine biosynthetic process via
                     aminoadipic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine biosynthesis protein LysW"
                     /protein_id="HNQ99626.1"
                     /translation="MPMLDTPYGRLDLDPAALMLGELVVTDEGAELEVVALDPLRFEL
                     APEEAEDWGE"
     gene            1338..>2094
                     /locus_tag="PKN52_06475"
     CDS             1338..>2094
                     /locus_tag="PKN52_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RimK family alpha-L-glutamate ligase"
                     /protein_id="HNQ99627.1"
                     /translation="MSPLPSAAADAMVAAAAGAIAPGTTVAPERSPVARQVVASAVAV
                     VYDRLREEERLLFDAFERLGVPFTPVYAPTLALELGAGPLPGVVLERCVSQWRGLSLA
                     HAYEAAGSTVINRPDVIRTCGDKLATSAALVRHGVPTPRTSLAVDAEAALVEADRLGY
                     PVVMKPLVGSWGRLVARAESRAALEALIEYKEVLGGPEHKVHYLQEYVEKPGRDIRAF
                     VVGDEVICAIYRSSEHWITNTARGGKASDCPLTD"
BASE COUNT          302 a          733 c          714 g          345 t
ORIGIN      
        1 ctgcgcgcga ccgacgacca ggagaccgcc gtcgccgtct tccgcaaaca cgaccgctcc
       61 gcgctgcccg tcgtcgactc gtctggcgtc atcgtcggca tcgtcacggt ggacgacgtc
      121 ctcgacgtgg ccgaggagga ggccacggaa gacatacaga tgctcggtgg ggtcgaggcc
      181 ctcgaggagc cgtacctgag catctcgctc gccagggtga tccgcaagcg cgcgttctgg
      241 ctcgtgctgc tcttcctcgg ccagaccctg acggccacgg ccatgggctt cttcgaggca
      301 gagatagcca gcgccgtcgt cctcgcactg ttcctaccgc tcatcatctc gtccggcggc
      361 aactccggct cccaggccgc ggccctcgtg actcgcgcca tggcgctcgg cgagatcggc
      421 gtgaaggatt ggtgggccat cgcgaagcgc gaggtgctgg ccgggctggc gctcggcgtg
      481 ttgctcggcg gcctcggctt cgcgcgcgtc gccgtctggg gcgtggtcct gcgcgcttac
      541 ggcgacgact ggttcctgat cggcgtcacc gtgttcatag cgctcatcgg cgtcgtcctc
      601 tggggctcgc tgaccggctc gctcctgccg atcgtcctca agcgccttgg cgcggacccc
      661 gcggcctcct cgaccccgtt cgtcgcgacc atcgtcgatg tcgtcggcat cctcatcttc
      721 ttcggcctcg ccaagctcgt tctgacgggc accctgctgt agcagcactc ctttcgaatc
      781 gaaagcataa cggggtcggc gggtgggctc gccttgcctc gtacggggtt ggccttggaa
      841 catcaaccgc aacgggccgg cgacgttcga ttcgatgatt tactcgatca ccatcgttaa
      901 actgccgact tgacatggtc gcacggcggc actaaactac tcctcggcct gtgggtcgcg
      961 gcgccctcca acggggcgtt ctactttcga aacgaaagca cggttccgct acatcgaact
     1021 caccggaacg caacaggttc cggccgggcg gaccgttcgc gcgacaggtc gagggccgtc
     1081 cgggtgccaa gcgtctagag cgccaggccg aaggacaggc ggcggaccga cggccggcgc
     1141 tcgacgggtg ccggctcgcg ggccgaggag gaagccatgc cgatgttgga cacgccctac
     1201 ggacgcctag acctagaccc agctgcgctc atgctcggcg agctcgtcgt gacggacgag
     1261 ggggccgaac tcgaggtcgt ggcgctcgac ccgctgcgct tcgagctggc cccagaggaa
     1321 gcggaggact ggggcgagtg agcccgctcc cctcggcagc cgcggacgcc atggtcgccg
     1381 cggcggcggg ggcgatcgcc cctggcacca ccgtcgcccc ggagcggagc ccggttgccc
     1441 gccaggtcgt cgctagcgcc gtcgccgtcg tctacgaccg gctgcgcgag gaggaacggc
     1501 tgctcttcga cgccttcgag cgcctgggag tccccttcac cccggtctac gcccctacgc
     1561 tggcgctgga gctcggcgcg ggtccgctcc ccggtgtcgt gctggagcgc tgcgtgtcgc
     1621 agtggcgcgg actgagcctc gcgcacgcgt acgaggctgc tggtagcacc gtcataaacc
     1681 gccctgacgt gatcaggacg tgcggcgaca agctcgcgac cagcgccgcc ctcgtgcgcc
     1741 acggcgtgcc aacgccccgc accagcctcg ccgtcgacgc ggaggccgcc ctggtcgagg
     1801 ctgaccgcct cggttatccg gtcgtcatga agcccctcgt ggggagctgg ggacggctcg
     1861 tggcgcgcgc cgagagccgg gcggcgttgg aggcactcat cgagtacaag gaggtactcg
     1921 gcggccccga gcacaaggtg cactacctcc aggagtacgt cgagaagccg ggtcgcgaca
     1981 tccgcgcttt cgtggtgggc gacgaggtca tctgtgccat ctaccgctcc tccgagcact
     2041 ggatcacgaa cacggcgcgt ggcggcaagg cgagcgactg cccgctgacg gacg
//
LOCUS       DAOQYE010000357         2091 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_61397_length_2091_cov_2.294695, whole genome shotgun sequence.
ACCESSION   DAOQYE010000357 DAOQYE010000000
VERSION     DAOQYE010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61397_length_2091_cov_2.294695"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..951
                     /locus_tag="PKN52_06480"
     CDS             <1..951
                     /locus_tag="PKN52_06480"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177256.1"
                     /note="catalyzes the interconversion of alpha-D-glucose
                     1-phosphate to alpha-D-glucose 6-phosphate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase, alpha-D-glucose
                     phosphate-specific"
                     /protein_id="HNQ99628.1"
                     /translation="GGASLGYWEPIASRWGLDITVVNDVVDPTFAFMPVDHDGKIRMD
                     CSSPYAMAGLIELEHRFDVAFGNDPDADRHGIVAPSSGLLNPNHYLAVAIDYLFTHRP
                     GWPAGAKVGKTLVSSSLIDRVAAGLGREVAEVPVGFKWFVPGLLGGDFAFGGEESAGA
                     TFLRMDGAPWTTDKDGLILGLLAAEIRAVTGEDPGRRYEALTGRFGAPTYARSDAPAG
                     KERKRVLGNLSPEAVLGAGRKELAGERILRVLSKAPSNGEPIGGIKVVTENGWFAARP
                     SGTEDVYKIYAESFAGPEQVARLQAEARALVQEQFELAGV"
     gene            complement(959..1792)
                     /locus_tag="PKN52_06485"
     CDS             complement(959..1792)
                     /locus_tag="PKN52_06485"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNQ99629.1"
                     /translation="MRILHPTDFSQPAQMALALARDLRRRTNGTLHVVHVQQRFETDG
                     ARLRPQLDSVNMELNRRVEEGRGQEVERLRGMLSHLASPDATSELVWGNPVVELLAMQ
                     DRFDLVVMGAHGANRFDNVFLGGVAGRFVRRSYLPVITVREEADVGRVQRVLVATDFG
                     AASWAAWGLVRGLADQGVELILAHVIDDERVRDDATHIQSITDKLSELSAGAASRIIV
                     RAGNPVTVLPELAVEIGADLIAIGIRQHRAAAGLVFGSRADGLIRSSKVPVLSVPLAD
                     E"
     gene            1974..>2091
                     /locus_tag="PKN52_06490"
     CDS             1974..>2091
                     /locus_tag="PKN52_06490"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNQ99630.1"
                     /translation="MYKKILMPTDGSASSDLAVTQGLELAKQLGAEVTFLHIL"
BASE COUNT          317 a          697 c          749 g          328 t
ORIGIN      
        1 ggtggggcgt cgctcgggta ctgggaaccc atcgcgagtc ggtggggcct cgacatcacg
       61 gtcgtcaacg atgtcgtgga ccccaccttc gcgttcatgc cggtggacca cgacggcaag
      121 atccgcatgg actgctcgtc gccttacgcg atggcaggcc tcatcgagtt ggagcaccgc
      181 ttcgacgtgg ctttcggcaa cgacccggat gccgaccggc acgggatcgt cgcgccgagc
      241 agcgggctcc tgaacccgaa ccactacctg gcggtggcca tcgactacct cttcacgcat
      301 cggcccggct ggccggccgg tgcgaaggtc ggcaagacgc tcgtctccag ctcgctgata
      361 gaccgcgtgg cggccgggct cggacgcgag gtggccgagg tgccggtcgg attcaaatgg
      421 ttcgtccctg gtctcctggg cggcgacttc gccttcggtg gcgaggagag cgccggcgcc
      481 accttcctcc gcatggacgg ggcgccgtgg accaccgaca aggatgggct catcctcggg
      541 ctgttggcgg ccgagatccg ggccgtgacg ggcgaggacc cgggtaggcg ctacgaggcc
      601 ctgacaggac ggttcggcgc cccgacctac gcccgcagcg acgcaccggc gggcaaggag
      661 cgcaagcggg tcctcggcaa cctgagcccg gaagccgtcc tcggggcggg acggaaggag
      721 ctggcggggg agaggatcct gcgggtcctc agcaaggcgc cgtcgaacgg cgagcccatc
      781 ggcggcatca aggtcgtgac cgagaacggt tggttcgccg cccgaccctc aggcacggag
      841 gacgtctaca agatctacgc ggagagcttc gctggcccgg agcaggtggc ccggctgcaa
      901 gccgaggcca gagcgctcgt tcaggagcag ttcgagctgg ccggggtctg agccgctctt
      961 actcgtcggc cagcggcacg ctcagcacgg ggaccttgct cgagcggatg aggccgtccg
     1021 cgcggctccc gaacacgagg ccggccgccg ccctgtgctg cctgatgccg atggcgatca
     1081 ggtccgctcc gatctccacg gcgagttccg gcaggaccgt caccggatta ccggcgcgca
     1141 cgatgatccg ggaggcggct ccggcggaga gctcactgag cttgtcggtg atcgactgga
     1201 tgtgagtggc gtcgtcccgc acgcgctcgt cgtcgatgac gtgcgccagt atcagttcga
     1261 cgccctggtc ggcgagaccg cgcaccagcc cccaggccgc ccaggaggcg gcgccgaagt
     1321 cggtggcaac gagcacgcgc tgcacgcgtc cgacgtcggc ttcctcccgc acggtgatga
     1381 ccggcaggta gctgcggcgc acgaagcgac cggcgacgcc gccgaggaac acgttgtcga
     1441 aacggttggc gccgtgggcg cccatgacca ccaggtcgaa gcggtcctgc atggccagca
     1501 gctcgacgac ggggttgccc cacacgagtt cgctcgtcgc gtccgggctg gcgaggtgcg
     1561 acagcatgcc gcgcagccgc tccacctcct ggccgcggcc ctcctcgacg cgccggttca
     1621 gctccatgtt gacgctgtcg agctgcgggc gcagcctggc gccgtcggtc tcgaagcgtt
     1681 gctgtacgtg cacgacgtgc aacgtgccgt tcgtgcggcg gcgtaggtcg cgcgccaacg
     1741 cgagcgccat ctgcgccggt tgggagaagt ccgtcgggtg caggatccgc atgcgtacct
     1801 ccaggcgttt gtcgcctcat catacgggcc ggggtgcggt gcgccgcctc gggtgctcca
     1861 ctcacgtccg tgtccgcgga actcctacgt gggtatgggc gtgatgacca cgcgctctca
     1921 tgacgtgcgg gattacgctg gtcttgggcg aggtgcccgt aaggaggccg tgtatgtaca
     1981 agaagatcct catgccgacg gacggcagcg ctagttccga cttggccgtg acgcagggtt
     2041 tggaactcgc caagcagctc ggggcggagg tcaccttcct ccacatcctc g
//
LOCUS       DAOQYE010000358         2090 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_61450_length_2090_cov_2.133170, whole genome shotgun sequence.
ACCESSION   DAOQYE010000358 DAOQYE010000000
VERSION     DAOQYE010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61450_length_2090_cov_2.133170"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..736)
                     /locus_tag="PKN52_06495"
     CDS             complement(<1..736)
                     /locus_tag="PKN52_06495"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HNQ99631.1"
                     /translation="MPGAPTPVRELTVDSLPVAVYASTEAMGLAAALAAREALASAIR
                     ERGEANVILATGNSQLAFLHALRELDGVDWRRVRAFHMDEYLGLPAGHPASFPRFLHE
                     HFVDHVPLGEFHPVPGDEDKAEENCRAYEALLRAHPVDLVALGWGENGHLAFNDPPYA
                     FFDDPHWVKVIELAEASRKQQVGEGHFPDLASVPTHAITLTIPALLAPKQVLCIVPEA
                     RKVPAVQACLYGPVSEDHPGSVLRTVQ"
     gene            complement(990..1406)
                     /locus_tag="PKN52_06500"
     CDS             complement(990..1406)
                     /locus_tag="PKN52_06500"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNQ99632.1"
                     /translation="MIVERIPTAWAEFQRHVGGLVRPTTEEEYERIVDIMRHVSDTYG
                     TKSEPYAALFDYLAGLAHTWELENEPALKDPDIEPYKVLAYLMEEHGVSQYQLAREGL
                     VNQGNLSRILSGERGISKALARKLAERFHVGVGVFI"
     gene            complement(1403..1702)
                     /locus_tag="PKN52_06505"
     CDS             complement(1403..1702)
                     /locus_tag="PKN52_06505"
                     /inference="COORDINATES: protein motif:HMM:NF021431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HNQ99633.1"
                     /translation="MNVIAPRTVREFWERHPRAQEPLRIWLKLMQRTDFANWAELKAA
                     FPRADYVSDANLVILNIGGNKYRLIADVSFRQNRVFIKRILTHAEYDKWNHGGRS"
     gene            1848..>2090
                     /locus_tag="PKN52_06510"
     CDS             1848..>2090
                     /locus_tag="PKN52_06510"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNQ99634.1"
                     /translation="MPIPAEVALLLESFDRNARVTRALLDTLSLEDLEYSDGVGGFNI
                     GQHLADIVNFRLSWLKRVSPEHAARVTDVTGETPTWL"
BASE COUNT          325 a          659 c          736 g          370 t
ORIGIN      
        1 gctgcacggt gcgcaggacg gagccggggt ggtcctcgct cacgggcccg tacaggcagg
       61 cctgcacggc ggggaccttg cgcgcttccg gcacgatgca gaggacctgc ttgggggcca
      121 ggagcgccgg gatggtgagg gtgatggcgt gcgtcggcac ggacgcgagg tcggggaagt
      181 gcccctcgcc gacctgctgc ttacggctcg cctcggccag ctcgatgacc ttcacccagt
      241 gggggtcgtc gaagaacgcg tagggcgggt cgttgaacgc gaggtggccg ttctcgcccc
      301 agccgagcgc cacgaggtcg accgggtggg cgcgcaggag cgcttcgtag gcgcggcagt
      361 tctcctcggc cttgtcctcg tcgccaggca cggggtggaa ctcgccgagc ggcacatggt
      421 cgacgaagtg ctcgtgcagg aagcgcggga agctcgccgg atgcccggcg ggcaggccca
      481 gatactcgtc catgtggaac gcccggacgc gccgccagtc gacgccgtcc agctcacgca
      541 gcgcgtgcag gaaggcgagc tgcgagttgc cggtcgcgag gatgacgttc gcctcgccgc
      601 gctcgcggat ggcggaggcg agcgcctcgc gggcagccag cgccgcggcc agacccatgg
      661 cctccgtgct ggcgtagacg gcgaccggga gggagtcgac cgttagttca cgtacgggtg
      721 ttggtgcgcc tggcatgcgg acatcctttc gctcgccgtc gaccgccccg tgagcgcgga
      781 tggcgcctgg gacgacccgt acccgttcaa ctggttcggg cgagtgcgtg ggccgccgcc
      841 gcgacccctg ttcgacacgt taggttaccg ctaacgcaag ctgctcgact gctgccaagg
      901 ataggtaggg gcggttgggg ctgtcaagct agggcgcggg tggacacgac cgcttgagcg
      961 cggccccgac cttcgctaga tgaggctcgt catatgaaca cgcccacgcc cacgtggaag
     1021 cgctccgcga gcttgcgcgc tagggccttg ctgatgccgc gctctcccga gagtatgcgg
     1081 ctcaggttgc cctggttcac gagcccttcc cgggccagct ggtactggct gacgccgtgc
     1141 tcctccatga ggtaggccag taccttgtag ggctcgatgt ccgggtcttt cagcgctggt
     1201 tcgttctcga gttcccaggt gtgcgccagg ccggcgaggt agtcgaagag agcggcgtac
     1261 ggctcgctct tcgtgccgta cgtgtcggaa acgtggcgca tgatgtcgac gatgcgctcg
     1321 tactcttctt cggtggtggg gcgcacgagg ccgccgacgt gccgctggaa ctctgcccag
     1381 gccgtaggta tgcgttcgac gatcatgaac gacctccatg gttccacttg tcgtactccg
     1441 cgtgcgtgag gatccgtttg atgaagacgc ggttctggcg gaagctaacg tcggcgatga
     1501 ggcggtactt gttgccaccg atgttgagga tgacgaggtt cgcatcgctc acatagtcgg
     1561 ctcttgggaa ggccgccttg agttccgccc agttcgcgaa gtcggttctc tgcatcagct
     1621 tgagccagat ccttaggggt tcttgcgcgc gcgggtggcg ctcccagaac tcgcgcacgg
     1681 ttctgggagc gatgacgttc acgcaaacag tgtatgtcag agcgacgtat ggcgctgtga
     1741 tatgggcgtg tgcgctcggc catccacccg ctagagtcat gctgcaagcc cagcaccgaa
     1801 cctacgaggc gcccgtcaag cgcgcgccga agaacggatg gccgaccatg ccgataccag
     1861 ccgaagtcgc actgctcctc gagtccttcg accgtaacgc ccgtgtaacg cgcgccttgc
     1921 tcgacaccct cagtctggag gacctcgagt acagcgacgg cgtgggcggc ttcaacatcg
     1981 gccagcacct ggccgacatc gtcaacttca ggctcagctg gctgaagcgc gtctcgcccg
     2041 agcacgccgc gcgcgtgacg gacgtgacgg gcgagacacc gacctggttg
//
LOCUS       DAOQYE010000359         2082 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_61813_length_2082_cov_2.519487, whole genome shotgun sequence.
ACCESSION   DAOQYE010000359 DAOQYE010000000
VERSION     DAOQYE010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61813_length_2082_cov_2.519487"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..692
                     /locus_tag="PKN52_06515"
     CDS             <1..692
                     /locus_tag="PKN52_06515"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNQ99635.1"
                     /translation="FAAEGAKVLGADWNEEALKAVVDEVTAVGGTMKGVRTNVAERAQ
                     VEAMVEAAVDAFGKLDVLVNNAGVMDLNQGVAELDDDLYRRVMGVNVDGPTFASRAAI
                     HAMGEQGGSILNIASVAGVGGGAAGAAYTMSKHAVIGLTRNTAFMYGPKGINCNAIVV
                     GAVATNIMASIDPTKLDQYGMSRSQRYYGLIPAQLQPDDIANAALFLVSDEAKMVNGA
                     LVTADGGWRAA"
     gene            754..1704
                     /locus_tag="PKN52_06520"
     CDS             754..1704
                     /locus_tag="PKN52_06520"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HNQ99636.1"
                     /translation="MSRTVGKAQRLTLLREELSRRPRRVVELAAQHGCTRRTIERDLA
                     TLAGQLGVDLRKDGSGRYYVPRPASVLNEVEALAVYSATRLLLHTGVGETHYRAALEK
                     LANQVPEPARTSLLRSVDDLKQGPEDRTLDLVAQAWFQGRVLRCEYDSANSGTTSARE
                     LEIYFFELNRRNLEPYVLAFDRTKNKKVVVLKLARMRNVTLKPDTYEIPADFDPKAKL
                     APAFGIVVGEEFELTLRASETVAKRMTENGDRSLRLGLDAGGGKRFVHVTATRDAQGR
                     PLEILPWLLGWGSSIEVVSPEFVRDALRAELALALAAYPA"
     gene            1736..>2082
                     /locus_tag="PKN52_06525"
     CDS             1736..>2082
                     /locus_tag="PKN52_06525"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HNQ99637.1"
                     /translation="MSMEFVCLAAGRGSRLGALSTYLQKCMYPVGLRPFLELTMAQAA
                     AAGGVRVHVVVGHLGEQVRAYFGDEFRGMRIEYVQQGEPLGTGHALGLVAARLGGVVL
                     SVLVWQADVYVPA"
BASE COUNT          338 a          712 c          734 g          298 t
ORIGIN      
        1 ggttcgccgc cgagggcgcc aaggtcctcg gcgccgactg gaacgaggag gccttgaagg
       61 ccgtcgtgga cgaggtgacc gctgtcggcg gcacgatgaa gggtgtaagg acgaacgtcg
      121 cggagcgcgc ccaggtagag gcgatggtcg aggccgccgt cgacgcgttc ggcaagctcg
      181 acgtcctcgt gaacaacgcc ggcgtcatgg acctcaacca gggcgtggcc gagctcgacg
      241 acgacctgta ccgccgcgtc atgggcgtga acgtcgacgg tccgacgttc gcgagcaggg
      301 cggccatcca cgccatgggc gagcagggcg ggagcatcct caacatcgcc agcgtggccg
      361 gcgttggcgg tggggccgcc ggcgcggcgt acaccatgtc aaagcacgcg gtcatcggac
      421 tgacccgcaa cacggccttc atgtacggcc ccaaggggat caactgcaac gcgatcgtgg
      481 taggggccgt ggctaccaac atcatggcga gcatcgaccc gaccaagctc gaccagtacg
      541 gcatgagccg ctcgcagcgc tactacggcc tgatcccggc ccagctgcag ccggacgaca
      601 tcgccaacgc ggccctcttc ctggtctccg acgaggccaa gatggtcaac ggggccctcg
      661 tgacggccga cggaggctgg cgcgcggcct gagcccgacc cacgggttta cggaggcgcc
      721 gcgccttgcc cacacgctgg tacgcttgag tgcatgagcc gtaccgtggg caaggcgcag
      781 cgcctcacgc tcctcaggga ggagctttcc cggcgaccca gacgggtggt cgaactcgcg
      841 gcccagcacg gctgcaccag gaggaccatc gagcgcgacc tcgccacgct cgcagggcag
      901 ctcggggtcg acctgcgcaa ggacggcagc ggccggtact acgtgccgcg gccggcttcg
      961 gtcctgaacg aggtcgaggc gttggccgtg tacagcgcca cccggctgct cctacacacc
     1021 ggggtgggcg agacgcacta ccgcgccgcc ctggagaagc tcgccaacca agtgccggag
     1081 cccgcgcgca cgtcgttgct ccgcagcgtc gacgacctca agcaaggtcc ggaggaccgt
     1141 actctcgacc tcgtcgcgca agcctggttc cagggccgcg tgctccgctg cgagtacgac
     1201 tcggccaact ccggcacgac gagcgcgcgc gagcttgaga tctacttctt cgagctgaac
     1261 cgccgcaacc tcgagccgta cgtgctcgcc ttcgaccgca cgaagaacaa gaaggtcgtg
     1321 gtgctcaagc tcgcgcgcat gcggaacgtc accctcaaac ccgacaccta cgagatccct
     1381 gccgacttcg accccaaggc caagctcgcc ccggcgttcg gcatcgtggt tggggaggag
     1441 ttcgaactca cgctgcgggc cagcgagacg gtggcgaagc gcatgaccga gaacggcgac
     1501 cgcagcctgc gcctcgggct ggacgccggt ggaggcaagc gcttcgtgca cgtgaccgcc
     1561 acgcgcgacg cccagggcag gccgctcgag atcctccctt ggctgctcgg gtggggatcc
     1621 tcgatcgagg tcgtctcgcc cgagttcgtg cgggacgctc tgcgcgccga gctggcgctg
     1681 gcgctcgccg cctacccggc ctgagcgtgg cgccgcggac caggggatgg acggcatgag
     1741 catggagttc gtctgtctcg cggcgggccg cggcagccgc ctgggcgcct tgagcaccta
     1801 cttgcagaag tgcatgtacc cggtcgggtt gcgcccgttc ctcgagctca ccatggcgca
     1861 ggccgcggcc gctgggggag tccgcgtgca cgtcgtggtc ggccaccttg gtgagcaggt
     1921 gcgcgcgtac ttcggggacg agttccgcgg catgcgcatc gagtacgtgc aacagggcga
     1981 accgctcggc acggggcacg ccctcgggct ggtggccgcg cgactcggcg gggtcgttct
     2041 gagtgtgctc gtctggcagg ccgacgtcta cgttccggcg ga
//
LOCUS       DAOQYE010000360         2080 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_61908_length_2080_cov_2.371852, whole genome shotgun sequence.
ACCESSION   DAOQYE010000360 DAOQYE010000000
VERSION     DAOQYE010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61908_length_2080_cov_2.371852"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..697)
                     /locus_tag="PKN52_06530"
     CDS             complement(<1..697)
                     /locus_tag="PKN52_06530"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HNQ99638.1"
                     /translation="MRLNRVLVRGLQEVAGLSETARGGGTGESGGTVVLRGPEAHHLR
                     DVLRLGVGAPVEAFDGLGNVARGEVSRADRDGVALRLGPLERSRSEAGLRLTVAVALL
                     KGDKLSDVVRQCTELGASGFVLTLTRQADVGELSAAKLQRLQRVAQEAARQCGRATVP
                     EIGGPSALAELTWSGAAFIADPRASVPLQDAAAATGHGGEALVLTGPEGGFTAAEVEG
                     LVARGAVAVNLGPR"
     gene            complement(688..1545)
                     /locus_tag="PKN52_06535"
     CDS             complement(688..1545)
                     /locus_tag="PKN52_06535"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HNQ99639.1"
                     /translation="MRAFRAPGITGESPEAALLWDLGCLGLQEEEGIDGVAVLAYFAI
                     EFDLPLAGEWVELPDVDHVGEYLASLESVRCGTLLVTPSHRQAVLERGEGVVWLDPGT
                     AFGTGHHETTAMALEALGGIELTGKTVLDLGSGSGLLAIGADRLGAERVYGLDIDPEA
                     VRVARENARLNRSRARFAIGTLGAAPAGLPGDEWSGPDRPAGRVPGLPGHFDVIAANL
                     FAELHAALFDEYASRLLPGGALVLTGILATHRALVERAKPACFSLAREEGRGDWLLLE
                     YRRTEAPCG"
     gene            complement(1542..>2080)
                     /locus_tag="PKN52_06540"
     CDS             complement(1542..>2080)
                     /locus_tag="PKN52_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HNQ99640.1"
                     /translation="ARMIAPEVGELVLVGRDRERLNRSADSLRKKYPHTRIIATTDVE
                     AVAGSDLIFTATSDPEPVIYAHHVKPGAWVYDLGRPVDVDESVRTVPGVELVPGGVVR
                     PPGNMRSRIDLRFGDGNVPACLAETMILTATRAYDRRSLGSATRSADIEFYLLEGERL
                     GFEIVTRDARLAPVPVTA"
BASE COUNT          284 a          801 c          727 g          268 t
ORIGIN      
        1 ctcgcggtcc taggttcacc gcgacggcgc ccctggccac gaggccctcg acctccgcag
       61 ccgtgaagcc gccttccggg ccggtcagca cgagcgcctc tccgccgtgc cccgtggccg
      121 ccgccgcgtc ctgcaacggc acgctcgcgc gcgggtcggc gatgaacgcc gcgcccgacc
      181 acgtcagttc cgccagggcg gacggcccgc cgatctcggg caccgtcgcc ctgccgcact
      241 gccgcgcggc ttcttgcgcc acgcgctgga ggcgctggag cttggccgcc gacagctcgc
      301 cgacgtcggc ctggcgggtg agagtgagga cgaaaccgga ggcgccgagc tccgtgcact
      361 gcctcaccac gtcgctcagc ttgtcgccct tcagcagcgc gacggcgacg gtcagccgca
      421 agccggcttc cgagcggctc cgctcgagcg gaccgagccg cagggcgacg ccgtcgcggt
      481 cggcccgcga gacctcgccg cgcgccacgt tgccaagccc atcgaaggcc tcgaccggcg
      541 cgcccactcc cagacggagc acgtcgcgta ggtggtgcgc ttccggaccg cgcaacacca
      601 ccgtgcctcc gctctcgccg gtgcccccgc cccgcgcggt ctcggagaga cccgcgactt
      661 cctggaggcc gcgcaccagc acgcggttca gccgcacggt gcctccgtgc gccggtactc
      721 gagcagcagc cagtcgccgc gcccctcctc gcgcgccagg ctgaagcagg cgggcttcgc
      781 acgctcgact agcgcccgat gggtggcgag gatgccggtc agcacgagcg cgccgcccgg
      841 caggagtctc gaggcgtact cgtcgaagag ggcggcgtgc agctcggcga agaggttcgc
      901 ggctatcacg tcgaagtgac cgggcaggcc cggaacgcga ccggccgggc ggtccggtcc
      961 ggaccactcg tcgcccggga gcccggcggg ggcggcgccg agcgtgccga tggcgaaccg
     1021 cgcgcgcgac cggttgaggc gcgcgttctc gcgcgccacg cgcaccgcct ccgggtcgat
     1081 gtccagcccg tacacgcgct ccgcgccgag ccggtcggcg ccgatcgcca gcagcccgct
     1141 ccccgaaccc aggtccagaa cggtcttccc cgtcagctcg atgccgccga gcgcctcaag
     1201 cgccatcgcg gtcgtctcgt gatgacccgt gccgaacgcg gtgccggggt cgagccatac
     1261 gacgccttcg ccgcgctcca gcacggcctg cctgtggctc ggggtcacga gcagcgtacc
     1321 gcagcgcacg ctctcgaggc tcgccaagta ctcgcccaca tggtccacgt cgggcagctc
     1381 gacccactca cccgccaagg gcaggtcgaa ctcgatcgcg aagtacgcga ggacggcgac
     1441 cccgtcgatg ccctcttcct cctgcagccc gaggcagccg aggtcccaca ggagcgcggc
     1501 ctccggcgac tcgcccgtga tacccggcgc cctgaaggcc ctcaagccgt gaccgggacc
     1561 ggcgcgaggc gcgcgtcgcg cgtgacgatc tcgaacccga ggcgctcgcc ctccaacagg
     1621 tagaactcga tgtcggccga gcgggtcgcg ctcccgaggc tccgcctgtc gtaggcgcgc
     1681 gtggcggtga ggatcatggt ctcggcgagg caggccggca cgttgccgtc gccgaagcgc
     1741 aggtcgatgc ggctacgcat gttgccaggc ggccggacga cgccgcccgg tacgagttcg
     1801 accccgggca ccgtgcgcac gctctcgtcc acgtcgacgg gccggcccag gtcgtagacc
     1861 caggcaccgg gcttcacgtg gtgcgcgtag atgaccggct cggggtcaga cgtggccgtg
     1921 aagatcaggt ccgagccggc gacggcctcc acgtcggtcg tcgcgatgat gcgcgtgtgc
     1981 gggtacttct tgcgcaggct gtcggctgag cggttcaggc gctcgcggtc gcgcccgacg
     2041 agcacgagtt ccccgacctc gggcgcgatc atgcgcgcaa
//
LOCUS       DAOQYE010000361         2079 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_61939_length_2079_cov_3.159585, whole genome shotgun sequence.
ACCESSION   DAOQYE010000361 DAOQYE010000000
VERSION     DAOQYE010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61939_length_2079_cov_3.159585"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="PKN52_06545"
     CDS             <1..516
                     /locus_tag="PKN52_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNQ99641.1"
                     /translation="VGPVFHDDFETQLRNEFEGVELGVTLPLATTLLAAAFFFVALQL
                     VGLPPLSGFIAKFGMLAAVVEAGSGGYAHAPASAAWWVVGVIVFSGFCVLVALMRKGV
                     NVFWAEPAGTTRVVRPLEAVPVAFLIALLVALTVLAQPTLRYTAAASAGLEARSSYLS
                     SVLQGPEGEEP"
     gene            513..998
                     /locus_tag="PKN52_06550"
     CDS             513..998
                     /locus_tag="PKN52_06550"
                     /inference="COORDINATES: protein motif:HMM:NF014012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HNQ99642.1"
                     /translation="MRRIVPHPLLAALLAGMWLLLQESLAPGDIALGVALGLVLSRVM
                     VALEQEAPSIKRPFVALGLFLVVSYDVVRSNFAVVAILADRRRKAHSGFVNVPLTIKS
                     PYALAALATIITATPGTFWAAYDSRTNVVTIHVLDLVDPDYWVRTVTQRYERRLRAVF
                     E"
     gene            995..1279
                     /locus_tag="PKN52_06555"
     CDS             995..1279
                     /locus_tag="PKN52_06555"
                     /inference="COORDINATES: protein motif:HMM:NF004812.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K+/H+ antiporter subunit F"
                     /protein_id="HNQ99643.1"
                     /translation="MTAQAILHWGLLAAHGLLGIGLVFAAARLIRGPRAQDRVLAFDA
                     VYLNAALQVVVFGMRAGQSGYLEAALVMTLLGFAGSSALAKFLMRGEVIE"
     gene            1276..1734
                     /gene="mnhG"
                     /locus_tag="PKN52_06560"
     CDS             1276..1734
                     /gene="mnhG"
                     /locus_tag="PKN52_06560"
                     /inference="COORDINATES: protein motif:HMM:TIGR01300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HNQ99644.1"
                     /translation="MTPFVAVIVVLLALFGAALAAVGSYGLLRLRSFYERAHPATLAA
                     TLGATSILLAAILFYSVSAGRFLPKALLLIVFIPLVNPVTTMLLARAALSRDRARRRP
                     TAVVEGSAEAEWLREADELVRAQAEAARQQAIVDDETAPAVATDRSEGEA"
     gene            1749..>2079
                     /locus_tag="PKN52_06565"
     CDS             1749..>2079
                     /locus_tag="PKN52_06565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99645.1"
                     /translation="MGLSNWQLLGLGVGAASLAITWGLYNNFMPLLLRDYVRSSGMRG
                     AIMGLDNVMAMILLPLLGTWSDRIDGPLGKRLPFLIVSMPLAAVLFALLPFAKAAFWT
                     LLAVDVAF"
BASE COUNT          253 a          773 c          677 g          376 t
ORIGIN      
        1 gtcggccccg tcttccatga cgacttcgag acgcagctgc gcaacgagtt cgaaggcgtc
       61 gagttgggcg tcacgctgcc gctcgccacc accctgctcg ccgccgcctt cttcttcgtg
      121 gcgctgcagc tggtcgggct gccgccgctc tccgggttca tcgccaagtt cggcatgctg
      181 gccgccgtcg tcgaggcggg gtccggcggc tacgcgcacg cgcccgcctc cgcggcctgg
      241 tgggtcgtcg gcgtcatagt cttctctggt ttctgcgtcc tggtggcgct catgcgcaag
      301 ggcgtcaacg tgttctgggc ggaaccggcc gggaccactc gcgtggtcag gccgttagag
      361 gccgtccccg tcgcgttcct catcgccctg ctcgtagcgc tgaccgtgct ggcccagccg
      421 acgttgcgtt acacggccgc ggcgagcgcc ggcctcgagg cgcgctccag ttacctgagc
      481 agcgtcctgc agggaccgga gggtgaggag ccgtgaggcg catcgtcccc cacccgctcc
      541 tcgccgcctt gctcgcgggc atgtggctcc tgctccagga gtcgctcgcg cccggcgaca
      601 tcgccctcgg ggtcgcgctc gggctcgtgc tctcgcgcgt gatggtggcc ttggagcagg
      661 aagcgccgag catcaagcgc cccttcgtag ccctcggtct gttcctggtc gtgtcttacg
      721 acgtggtgcg ctccaacttc gccgtcgtcg ccatcctggc ggaccggcgt cgtaaggccc
      781 actccggctt cgtgaacgtg cctctgacca tcaagagccc ttacgcgctc gccgcgctcg
      841 ctacgatcat caccgccacg ccgggcactt tctgggccgc ctacgactcc cgcacgaacg
      901 tcgtgacgat ccacgtcctc gacctcgtcg accccgacta ctgggtgagg acggtaacgc
      961 aacgttacga gcggcgcctc cgggcggtgt tcgaatgacg gcgcaggcca tcctccactg
     1021 gggcctcctg gcggcccacg ggctgctggg catcggcctc gtcttcgccg ccgcacggct
     1081 catcaggggc ccgcgcgcac aggaccgcgt cctggcgttc gacgcggtct acctcaacgc
     1141 ggcgctgcaa gtggtcgtgt tcggcatgcg tgccggccag agcggctacc tcgaggcggc
     1201 cctcgtcatg acgctcctcg gcttcgccgg cagctcagcc ctcgcgaagt tcctcatgcg
     1261 cggggaggtc atcgagtgac ccccttcgtg gccgtcatcg tcgtcctgct cgccctcttc
     1321 ggcgcggcgc tcgcggccgt cggttcgtac ggcttgctgc ggctgcgctc cttctacgag
     1381 cgcgcccacc cggcgacgct ggccgccacc ctcggcgcca cgtccatcct cctcgcagcg
     1441 atcctcttct actccgtctc ggccggcagg ttcctgccca aggccttgct cctgatcgtc
     1501 ttcatccccc tcgtgaaccc ggtcacgacg atgctactcg cgcgcgcggc gctctcgcgc
     1561 gacagggcgc ggcggcgccc gaccgcggtg gtggagggca gcgccgaagc cgagtggctg
     1621 cgcgaggccg acgagctcgt gcgcgcgcag gccgaggcgg ccaggcaaca ggccatcgtc
     1681 gacgacgaga cagcgccggc cgtggcgacc gaccgctccg aaggtgaggc gtgaaccgag
     1741 gcggcagggt gggcttaagc aactggcagc tcctcggcct cggcgtcggc gcagccagtt
     1801 tggcgataac ctggggcctc tacaacaact tcatgccgtt gctcctacgc gactacgtgc
     1861 gttcgagcgg catgcgcggc gccatcatgg gcctcgacaa cgtgatggcc atgatcctcc
     1921 tcccgctact cggcacctgg tcggatagga tcgacggccc gctcggcaaa cgcctgccgt
     1981 tcctgatcgt gagcatgccg ctggccgccg tcctgttcgc gctcctgccc ttcgcgaagg
     2041 ccgccttctg gaccctactg gcggtcgacg tcgcgttcc
//
LOCUS       DAOQYE010000362         2077 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_62039_length_2077_cov_3.961919, whole genome shotgun sequence.
ACCESSION   DAOQYE010000362 DAOQYE010000000
VERSION     DAOQYE010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62039_length_2077_cov_3.961919"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="PKN52_06570"
     CDS             <1..298
                     /locus_tag="PKN52_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99646.1"
                     /translation="HWDDIGDWVALERLLTPEDGPGGANTVVGRHVGLDTSRNIVYTE
                     DPDDVIVTLGVHDLVIVKRGNAVLLVHKTRVQELKALLADERLAALMTRGAEES"
     gene            452..670
                     /locus_tag="PKN52_06575"
     CDS             452..670
                     /locus_tag="PKN52_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99647.1"
                     /translation="MAKRRTTVTLSSDLLKAVKIRAARTGQREDEVIEAALRRGLGLD
                     VFERLWARNDLEEDEAMELALAAQRSVT"
     gene            complement(674..>2077)
                     /locus_tag="PKN52_06580"
     CDS             complement(674..>2077)
                     /locus_tag="PKN52_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNQ99648.1"
                     /translation="AKAWAKTPAPIRGQVIGLVGQAIEREKEPLSRLITREMGKTLKE
                     ARGDVQEAIDTCYFFQSEGRRLYGQTVPSEMPRKDLMTYRRPLGVVGIVTAGNFPVAV
                     PSWKIIPALVTGNTIVWKPSEDVPACSYAFAKILLGAGLPAGVFNVVFGGGKDAAGQY
                     LIELMDDGLVDKLAFTGSSTVGRYVGEVAGRNLQHPTLELGGKNPLVVMRDADLDNAV
                     QGAVFSAFATGGQRCTSAGNVIVDAPVYDEFKKRYLAAVEKIRIGDPLKTEDVLYGPF
                     INERFFRRWEQQYEWGKADGATLLYGKGRITADSKPAGFVGDPNAGFYGWPTVWEGVK
                     PGMKLFQEEIFGPTINLVKVDGIEEAIATANAVAYGLSSSIYTNNREWAYAFKDEIQA
                     GMTSINNTTNGAEAHMPFGGVKASGNGTRESGIWVLDSYTYWHGVNDDISGKLQLAQM
                     DTAYVEPQAAVSVAELF"
BASE COUNT          332 a          655 c          714 g          376 t
ORIGIN      
        1 ccactgggac gacatcggcg actgggtggc tttggagagg ctcctcacgc cggaggacgg
       61 gccgggtggc gccaacaccg tggtggggcg tcacgtcggg ctggatacgt cgcgcaacat
      121 cgtctacacg gaggacccgg acgacgtgat cgtcacgctc ggcgtacacg acctggtgat
      181 cgtcaagcgc gggaacgccg tgctcctggt gcacaagacg cgcgtgcaag agctgaaggc
      241 cctgctcgcc gacgagcggc tggcagcgct gatgacgcgg ggcgcggaag agtcatagca
      301 ctcaagccag gtcagccata cctgccgcct tctgctccat ggatgcggca cccacctgcg
      361 caagctggag cttgcctgaa ggcgtcgtga tgaatgtggt tcatgctttg atgcgattcc
      421 atatgtccat atgtgatgcc aagatgcagt gatggcgaag aggcgcacga ccgtcacgct
      481 gagcagcgac ctcctgaagg ccgtgaagat ccgcgcagct cgcacgggcc agcgtgagga
      541 cgaggtcatc gaggcggcac ttcggcgcgg gctaggtctc gacgtgttcg agaggctatg
      601 ggcgcggaac gacttggaag aagacgaggc gatggaactg gcccttgccg cccagcggtc
      661 ggttacgtag agcctagaac agttccgcca cgctcaccgc cgcctgcggc tccacgtaag
      721 cggtgtccat ctgcgcgagc tggagcttgc ctgagatgtc gtcgttgacg ccgtgccagt
      781 acgtgtagct gtccagcacc cagatgccgc tctcgcgcgt gccgttgccg ctcgccttca
      841 caccaccgaa cggcatgtgg gcttccgccc cgttcgtggt gttgttgatg ctcgtcatgc
      901 cggcctggat ctcgtccttg aaggcgtatg cccattcgcg gttgttcgtg tagatgctgc
      961 tcgagagccc gtaggcgacg gcgttggcgg tggcgatggc ctcctcgatg ccatcgacct
     1021 taacgaggtt gatggttggt ccgaagatct cctcctggaa gagcttcatg ccgggcttga
     1081 cgccctccca cacggtcggc cagccgtaga agccggcgtt cgggtcgccc acgaaccccg
     1141 cgggcttcga gtccgcggtg atgcggccct tgccgtagag gagcgtcgcg ccgtctgcct
     1201 tgccccactc gtactgctgc tcccagcgcc tgaagaagcg ctcgttgatg aacgggccgt
     1261 agaggacgtc ctccgtcttg agcgggtcgc cgatgcggat cttctcgacc gcggcgaggt
     1321 agcgcttctt gaactcgtcg tagacgggcg cgtcgacgat gacgttgccc gcgctcgtgc
     1381 agcgctgccc gcccgtggcg aatgccgaga agacggcgcc ctggacggcg ttgtcgaggt
     1441 cggcgtcgcg catgacgacg agcgggttct tgcccccgag ctcgagggtc gggtgctgca
     1501 ggttgcggcc cgccacctcg ccgacgtacc gcccgaccgt gctggagccc gtgaaggcga
     1561 gcttgtcgac gaggccgtcg tccatgagct ctatcaggta ctgccctgcc gcgtccttgc
     1621 cgccgccgaa cacgacgttg aacacgccgg ccggcagccc ggcgccgagg aggatcttgg
     1681 cgaaggcgta cgaacaggcc ggcacgtcct ccgacggctt ccagacgatc gtgttgccag
     1741 tgacgagggc ggggatgatc ttccaactcg gcacggcgac aggaaagttg ccggcggtga
     1801 cgatgccgac gacgccgagc ggccggcgat aggtcatgag gtccttgcgc ggcatctcac
     1861 tcgggaccgt ctggccgtag aggcgcctgc cctcactctg gaagaagtag caggtgtcga
     1921 tggcctcttg gacgtcgccg cgggcctcct tgagggtctt gcccatctcg cgcgtgatga
     1981 ggcgggagag cggctccttc tctcgctcga tggcctgacc gacgaggccg atgacctgac
     2041 cgcggatggg agcgggcgtc ttggcccacg ccttggc
//
LOCUS       DAOQYE010000363         2074 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_62216_length_2074_cov_1.826152, whole genome shotgun sequence.
ACCESSION   DAOQYE010000363 DAOQYE010000000
VERSION     DAOQYE010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62216_length_2074_cov_1.826152"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="PKN52_06585"
     CDS             <1..252
                     /locus_tag="PKN52_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HNQ99649.1"
                     /translation="DVIRSHHERWDGGGYPDGLANTDIPFSARMFSLVDVYDALLSAR
                     PYKAAWSHERAVAELTEQSGRQFDPELVPTFLEIVARGG"
     gene            complement(291..1424)
                     /locus_tag="PKN52_06590"
     CDS             complement(291..1424)
                     /locus_tag="PKN52_06590"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HNQ99650.1"
                     /translation="MRTFLSRIDRYLLRESLPPFLFGSVLYASLAVVSVTIPRLQWIV
                     GAPAGKLGVWLLVQLPQALVQTLPIAVVLAVLLAFGRLASENELLALQAGAVPVRRVA
                     GVFVGLGLACAVAVLAINQWVLPVTNTLVTKQYWELTSGQTGLFRLAQQALPVDDFTL
                     HFDTAAERGTLMRNVRIERWDGDDLTILRAREGRFEGVNLVLSGYRIDALDHAALDGA
                     TDDPAAALARLIRLRNVPADPDSELTITTSVTLDELVTRFGAGGFEDYRSITRLWQDS
                     HLTSASATERRKSAALMHRKLAEPFTNLALLLVAVPLSLTYARTRGVAFGLSLVVTLL
                     WYLFYTFGQLFAQTGQLPVWLGAWLGNIVFAALGLVLLARRTR"
     gene            complement(1421..>2074)
                     /locus_tag="PKN52_06595"
     CDS             complement(1421..>2074)
                     /locus_tag="PKN52_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99651.1"
                     /translation="VTYDAFPTPTVPVGFKRLRGSGSLTLASDPWTVALTATVETAGL
                     MNALPDVGAFVEVDLSATRHGWPELAAGAPYGALELGVNAQYGLLPASTGLERLELRA
                     GVPLAFETVELRPFVAVDFAGLLHGGSPKLSGYGLDATFITCCGSFTLGALNNRGAWG
                     ASIAVDLERRPGAAGGAATPTQPAPVVDGEENDAQPGTEPVEPDGPDEAPGIIPVAP"
BASE COUNT          327 a          722 c          757 g          268 t
ORIGIN      
        1 gacgtgatcc gtagccacca cgagcggtgg gacggcggcg gctatccaga cgggctggcc
       61 aacaccgaca tcccgttctc tgctcgcatg ttcagcctcg tggacgtcta tgacgccctc
      121 ctcagcgccc ggccgtacaa ggcggcatgg agccacgaac gcgccgtggc ggaactcacg
      181 gagcagtctg gacggcagtt cgacccggag ctggtgccga cgttccttga gatagtcgct
      241 cgcgggggct gatgcgcgcc cgacggcgca cgcccagggc gacgccgtgc tcagcgcgtc
      301 cttcgagcca gcaataccag ccctagcgcg gcgaacacga tgttcccgag ccacgccccc
      361 agccacacgg gcaactggcc cgtctgcgcg aagagctggc cgaaggtgta gaagagatac
      421 cacaggagcg tgacgacgag gctcaggccg aacgccacgc cgcgcgtgcg cgcgtacgtg
      481 aggctgagcg gcaccgcgac gagcagcagc gcgaggttcg tgaacggctc ggcgagcttg
      541 cggtgcatga gggcggccga cttgcgccgc tccgtggccg aggcggacgt caggtgcgag
      601 tcttgccaca gccgggtgat ggagcggtag tcctcgaagc cgcccgcgcc gaagcgggtc
      661 acgagctcgt caagggtcac gctcgtcgtg atggtcagct ccgagtccgg gtcggccggc
      721 acgttgcgca gccttatcag gcgcgccagg gccgccgccg ggtcgtcggt ggcgccgtcc
      781 agcgcggcgt gatcgagggc gtcgatgcgg tagccggaca gcaccaggtt cacgccctcg
      841 aagcggccct cgcgggcccg caggatggtg aggtcgtcgc cgtcccaacg ctcgatgcgc
      901 acgttgcgca tgagggtgcc gcgctcggcg gccgtgtcga agtgcagcgt gaagtcgtcg
      961 acgggcagcg cctgctgcgc caggcggaac agaccggtct gtccggaggt gagctcccag
     1021 tactgcttcg tcacgagggt gttcgtgacc ggcagcaccc actggttgat ggccaatacg
     1081 gccaccgcgc acgccagccc caggcccacg aacacgccgg ccaccctgcg aacgggcacc
     1141 gccccggcct gcagggccag cagctcgttc tcgctcgcca ggcgcccgaa cgccagcagc
     1201 acggccagca cgacggcgat ggggagcgtc tgaacgagcg cctgcggcag ctggacgagc
     1261 agccacacgc cgagcttgcc ggccggcgcc cccacgatcc actggagccg cgggatggtg
     1321 acgctcacca cggcgaggct cgcatagagg accgagccga agaggaacgg cggcaggctc
     1381 tccctgagca ggtagcggtc tatgcgcgag aggaacgttc tcatggggcg accggaatga
     1441 tacccggcgc ctcgtcgggg ccgtctggct cgaccggctc cgtgccgggt tgggcgtcgt
     1501 tctcctcgcc gtccacgacc ggcgcgggct gggtcggggt agccgccccg cccgcggcgc
     1561 ccgggcgccg ctcgaggtcg acggcgatcg acgcgcccca ggccccccgg ttgttgagcg
     1621 cgcccagggt gaacgacccg cagcatgtga tgaaggtggc gtccagcccg tagccgctca
     1681 gcttcggaga gccgccgtgc aagaggcccg cgaagtccac cgccacgaac gggcgcagct
     1741 ccacggtctc gaaggcgagg ggcacgcccg ccctcagctc caggcgctca aggccggtgg
     1801 acgcggggag caggccgtac tgggcgttca ccccgagctc gagcgcgccg tacggcgcgc
     1861 ctgccgccag ctccggccaa ccgtggcgcg tggcgctcag gtcgacctcg acgaaggcgc
     1921 cgacgtctgg gagggcgttc atcaggccgg ccgtctcgac ggtggcggtc agggcgaccg
     1981 tccacgggtc ggacgcgagc gtgaggctgc ccgagccccg cagccgtttg aagccgacgg
     2041 gcacggtcgg cgtagggaac gcgtcgtaag tgac
//
LOCUS       DAOQYE010000364         2073 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_62242_length_2073_cov_2.792369, whole genome shotgun sequence.
ACCESSION   DAOQYE010000364 DAOQYE010000000
VERSION     DAOQYE010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62242_length_2073_cov_2.792369"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1232
                     /gene="thrS"
                     /locus_tag="PKN52_06600"
     CDS             <1..1232
                     /gene="thrS"
                     /locus_tag="PKN52_06600"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HNQ99652.1"
                     /translation="LLDKLGAELDIFVLDEDVGPGLPLWLPRGAIMIEEIERLAKETE
                     EAAGYQRVRSPHLTKEQLFLKSGHLPYYAESMYPPMVFEDGDKYYVKPMNCPFHHKIY
                     AARPHSYRDLPLRLAEYGTCYRYEDSGALMGLMRVRSMQMNDAHIYCTEDQFEEEFLK
                     VVDLYTHYFELFGIDDYQMRLSKHAPEGLGKKYVNEPELWVKTEAMVRDALVSAGVPF
                     VEEEDEAAFYGPKIDVQIKSAIGREFTLATNQVDFAQPARFELTYVDENNERKTPLCL
                     HRAPLSTHERLIGFLIEHFAGHFPVWLAPEQVRIVPIADRHTEYARGLQQRLGAAGLR
                     GQVDDSNERMNAKIRDAELYKVPYIVVVGDKEAAADEVSFRSKQEPDRQGVSAEAFIA
                     HLKDAHARRLLKIEPLG"
     gene            complement(1352..1930)
                     /locus_tag="PKN52_06605"
     CDS             complement(1352..1930)
                     /locus_tag="PKN52_06605"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNQ99653.1"
                     /translation="MSDLLAGFQPVAPLSVERQTRFPAAEQVVDQAGKEYRALIKTNK
                     GDIVLELYADKAPRTVNNFVFLALNGYYDGVVFHRVLEDFMAQTGDPTGTGTGGPGYT
                     FADEFHPDLRHRGAGVLSMANAGPGLRGEGTNGSQFFITFVDTPWLDDKHSVFGKVIG
                     GADVLDRIQRIDPSRRGGAQPDVMEKVEIYAR"
BASE COUNT          363 a          671 c          702 g          337 t
ORIGIN      
        1 cgctgctcga caagctcggc gccgaactcg atatcttcgt gctcgacgag gacgtcggcc
       61 cgggcctgcc gctctggctg ccacgcggcg ccatcatgat cgaggagatc gagcgcctgg
      121 ccaaggagac ggaggaagcg gccggctacc aacgcgtacg cagtccgcac ctcaccaagg
      181 agcagctctt cctgaagtcg ggacacctgc cgtactacgc ggagtccatg tacccgccga
      241 tggtcttcga ggatggcgac aagtactacg tcaagcccat gaactgcccg ttccaccaca
      301 agatctatgc ggccaggccg catagttacc gcgacctacc gctcaggctg gccgagtacg
      361 ggacctgcta tcgctacgag gactcgggcg cgctcatggg tctgatgcgc gtgcgctcca
      421 tgcagatgaa cgacgcgcac atctactgca ccgaggacca gttcgaggag gagttcctca
      481 aggtcgtcga cctctacacc cactacttcg agctcttcgg gatcgacgac taccagatgc
      541 gcctctccaa gcacgcgccg gagggcctcg gcaagaagta cgtcaacgag cctgagctat
      601 gggtcaagac cgaggccatg gtgcgagacg cgctggtgag cgccggagtg ccgttcgtgg
      661 aagaggagga cgaggccgcg ttctacgggc cgaagatcga cgtccagatc aagagcgcca
      721 tcgggcgtga gttcaccctc gccaccaacc aggtcgactt cgcgcagccg gcgcgcttcg
      781 aactcaccta cgtcgacgag aacaacgagc gcaagacccc cctgtgcctg caccgggcgc
      841 cgctgtcgac ccacgagcgc ctcatcggct tcctgatcga gcacttcgcc gggcactttc
      901 ccgtctggct ggcccccgaa caggtgcgca tcgtgccaat cgccgaccgc cacaccgagt
      961 acgcccgcgg gcttcagcag cggctgggtg ccgccgggtt acgcggtcag gtagacgaca
     1021 gcaacgagcg catgaacgcc aagatccgcg acgccgagct ctacaaggtg ccgtacatcg
     1081 tcgtggtggg cgacaaggag gccgcggccg acgaggtgtc gttcaggagc aagcaggagc
     1141 ccgatcgcca aggagtgagc gccgaggcgt tcatcgcgca tctaaaggac gcgcatgcgc
     1201 ggcggctgct gaagatcgag ccgctcggct gaccggctcg cccccggagc cggcgtctgc
     1261 cgtgtacggg tccagggcga tccgagagtt ggggcggcgg tcacgtaacc gccgccccaa
     1321 ctctcgggcg aggtagtgcc agcggccgtc ctcagcgcgc gtagatctcg accttctcca
     1381 tcacgtccgg ctgggcgccg ccgcgcctgc tcgggtcgat gcgctggatg cggtccagga
     1441 cgtcggcgcc gccgatgacc ttgccgaaga cgctgtgctt gtcgtccaac cacggggtgt
     1501 cgacgaaggt gatgaagaac tgcgaaccgt tcgtgccctc gcccctcagg cctgggccgg
     1561 cgttggccat gctgaggacc ccggcgccgc ggtggcgcag atcggggtgg aactcgtctg
     1621 cgaacgtgta gcctggcccg ccggtgccgg tacccgtcgg gtcgccggtc tgcgccatga
     1681 agtcttccag gacacggtgg aacaccacgc cgtcgtagta gccgttgaga gcgaggaaga
     1741 cgaagttgtt gaccgtgcgc ggcgccttgt cggcgtagag ctcgagcacg atgtcgccct
     1801 tgttcgtctt gatgagggcg cgatactcct tgcccgcctg gtcgacgacc tgctcggcgg
     1861 ccgggaagcg cgtctggcgc tcgacggaga ggggggcgac gggttggaag cctgcgagta
     1921 ggtctgacat ctagttccct tcggcggtgc cgttctgttg gatcaggtga ggtggcttct
     1981 ggatcaggtg aggcggcttc ggttcagcgc gggcgcgcgg cgatcactac ggtgaggagt
     2041 tcgtcaggcg ccggccagaa gccgaacgcc ggg
//
LOCUS       DAOQYE010000365         2073 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_62250_length_2073_cov_2.507929, whole genome shotgun sequence.
ACCESSION   DAOQYE010000365 DAOQYE010000000
VERSION     DAOQYE010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62250_length_2073_cov_2.507929"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="PKN52_06610"
     CDS             <1..478
                     /locus_tag="PKN52_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99654.1"
                     /translation="GWWNHTDKDTFDTLDEAVLERDGRFWTGLIHDLVSAEPLPHLAA
                     PAVEELRARFNTMLAEGEDPAELGRVRPLLDRLAERAVWLDGLQAVTEEALLARNEAL
                     LRLSRHVTPLRATVVGKYGQDSYGLSDLKEPVPMLAPLSRYRQLAADDPERHLLRT"
     gene            663..1382
                     /locus_tag="PKN52_06615"
     CDS             663..1382
                     /locus_tag="PKN52_06615"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HNQ99655.1"
                     /translation="MSTTLEKALQLVELVSDGDLSLQELCQASGFSRSTTHRLAASLV
                     KYRYLESKEQRYSLGYRLLELGEKKKASLRFTKVAKPIAHNYCERTGETVHIAVLDGS
                     YSVIVDRIVGSRQLQVNAYIGQRTPAYMTGVGKAMIASQPEHEWLYFLSGIGRRERAR
                     LLIEFSDIKERGYALDLEESASGVACVATTVYSPSGQAIAGVSFNGAIAYLTPERLVE
                     LAPTIRGCAREIEKAMAASGT"
     gene            1492..>2073
                     /locus_tag="PKN52_06620"
     CDS             1492..>2073
                     /locus_tag="PKN52_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99656.1"
                     /translation="MTRRVVFGFVLFLAVLTVPACRPGGGTPDPGALRELTVAVSLPT
                     GVSAGTAAVATPYGVNPLTGGTGKAVVTGAGATLATVQRGSDTLLIGFVSDERATLSV
                     RTTAEALAFYALQGQFLEPAMQASLLDFLSKSAAIEPVAALVQAAVLDDPPRVSASDP
                     GIDAALADMVASFSSTDVAPSVAANPSRSPPRWG"
BASE COUNT          379 a          668 c          659 g          367 t
ORIGIN      
        1 cggctggtgg aaccacaccg acaaggacac gttcgacacg ctcgacgagg ccgtgctgga
       61 gcgcgacggc cgcttctgga caggcctgat acacgacctg gtgagcgccg agcccctgcc
      121 ccatctggcc gcgccggcgg tcgaggagct gagagcgcgc ttcaacacga tgctggcgga
      181 aggcgaggac ccggccgaac tgggccgcgt acggccgctg ctcgaccgac tggcggagcg
      241 cgcggtgtgg ctggatggac tgcaggccgt taccgaggaa gccctgcttg cccgcaacga
      301 ggcactcttg cgcctgagcc gccacgtcac cccgctgcgc gccaccgtgg tgggcaagta
      361 tggccaggac agctacgggc tcagcgacct caaggagccc gtccccatgc tcgccccgct
      421 gagtcgttac cggcagctgg cggccgacga cccggaacgg cacctcctgc gcacgtagct
      481 gctgcgagtc aggcatcgct tcaccgacgc tctcattgcc gccatcgggg tcatagatac
      541 cctcgagtcg aagttgcggt cggcgccagc aggctagaca agccccatcg ctgtatcgcg
      601 gcctcgatgg tcgggcggtc tagggttcca gcagtttggt gctttcgttc taggatatcg
      661 gcgtgagcac aactctagag aaggcgctcc agctcgttga gcttgtgagt gatggggatc
      721 tgtcgcttca ggaactctgc caggcatcag gattctcccg cagcaccact caccgcctgg
      781 ctgcatcgtt ggtcaagtac cgttacttag aaagcaagga gcaacgctat agcctcggct
      841 accgcctcct cgagcttgga gagaagaaga aggccagtct ccggttcacc aaggttgcca
      901 agccgatcgc ccacaactac tgcgagcgga ctggcgaaac tgtgcatatt gccgtcctcg
      961 acggcagtta tagtgtgatt gtcgaccgta tagtcggcag taggcagctt caggtcaacg
     1021 cttacatagg tcagcgtaca cctgcgtaca tgacgggtgt gggtaaagct atgatcgcca
     1081 gtcagccgga acatgaatgg ctttacttcc tgagcggtat cggacgtagg gaacgggcgc
     1141 gtctgctgat cgaattctcc gacatcaaag agcggggcta cgcgctcgac cttgaggaga
     1201 gcgccagcgg ggtcgcctgt gtcgcaacca ctgtatatag cccctccggc caggcaatag
     1261 caggggtgag tttcaacggc gccatcgcct atctaacgcc ggaacggctg gtggagctgg
     1321 caccgacgat cagagggtgc gccagggaga tcgagaaggc catggcagcc tcggggacat
     1381 aaacgctaag cgtaagctgg ctcgccaaca gagacacgcc tgcccaagtg cgttaatctg
     1441 tctttaatct tcttggccta tactctcacc cggggataac ggaggatagc gatgactcgt
     1501 cgtgtcgtct tcgggttcgt cctgtttctt gcggtgctca ccgtgcccgc ctgtcgcccg
     1561 ggcgggggta cgcccgatcc gggggcgctc cgcgaactga cggtggcggt ctcgctaccg
     1621 acgggcgtca gcgctggcac tgcggcagta gccacaccgt acggcgtgaa ccccttgacc
     1681 ggcggcacgg ggaaggcggt cgtcaccggc gccggagcga ccctcgctac ggtgcaacgc
     1741 ggtagcgaca cgctcctcat agggttcgtg tcggacgagc gcgccaccct gagcgtgcgc
     1801 accaccgcgg aggccctcgc cttctacgcc ctccaaggcc agttcctcga accggccatg
     1861 caggctagcc tcctcgattt cttgtccaag agcgccgcca tcgaaccggt ggccgccttg
     1921 gtccaggcag ccgttctcga cgacccgccc agggtaagtg ccagcgatcc gggcatcgac
     1981 gccgccctgg cagacatggt cgcctccttc tcctccacag acgtggcccc cagcgtggcg
     2041 gcgaatccat cgagatcgcc gccacgctgg ggg
//
LOCUS       DAOQYE010000366         2070 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_62399_length_2070_cov_2.329529, whole genome shotgun sequence.
ACCESSION   DAOQYE010000366 DAOQYE010000000
VERSION     DAOQYE010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62399_length_2070_cov_2.329529"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /locus_tag="PKN52_06625"
     CDS             <1..434
                     /locus_tag="PKN52_06625"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNQ99657.1"
                     /translation="RHTQLGTVGIYDSKLTEAQEHFEEAIRIDPRHYRALTNLGNVAL
                     EQGRVDEAIEHYQAALKLNEEFANAHHNLGVAYRRKGHLNKSVKSLRRAQRSQQRHDA
                     AVARESLGKWTGKSGGKYLKWLLWGAAAIGAYFILKSMGYI"
     gene            complement(460..675)
                     /locus_tag="PKN52_06630"
     CDS             complement(460..675)
                     /locus_tag="PKN52_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99658.1"
                     /translation="MPYGFTIQGEAARVRELADIREAIVERVAGCEDAVLLGRIERLL
                     DDADDGERLLELDDATIEEVLRGLLDD"
     gene            complement(706..>2070)
                     /gene="glmS"
                     /locus_tag="PKN52_06635"
     CDS             complement(706..>2070)
                     /gene="glmS"
                     /locus_tag="PKN52_06635"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HNQ99659.1"
                     /translation="RVAGAYALVVAHAEHDLLVAARNTSPLVIGLGTDENYLASDVPA
                     LLPYTRDVIYLLDGDVAELGRDGVVITDLQGVRQARETNRVEWDAEAAEKGGYEHYML
                     KEIYEQPTVLQNTLFGRFTHGGLDVELGLNLDPHAIDRVVITAAGTASYAGEVGRTLL
                     RRMARLDTVVEVASEFRYSEPVVNERTLCVVVSQSGETIDTLEAMREAKRRGARTLAI
                     LNAKGSSISREADDVLYIHAGPEIGVASTKAYLGMVAAFALLSFWFARERGYLDDDAA
                     REYVRALRELPGQVERTLQVRDHVAQIAEQIKDARSSLFLGRGINAATALEGALKLKE
                     ISYVHAEAYPMGEMKHGPIALVDRDLPVVAVATASPLYDKTVSNLQEVRAREGRLIVV
                     ADEDDAKIGQHAEFVIPVPRTLELLSPILNVVPLQLLSYEVARALGRDIDQPRNLAKS
                     VTVE"
BASE COUNT          322 a          686 c          738 g          324 t
ORIGIN      
        1 aacgccacac gcagttgggc acggtcggca tctacgactc gaaactgacc gaggcgcagg
       61 agcacttcga ggaagccatc cggatcgacc cgcgccacta ccgggcgctg acgaacctcg
      121 gcaacgtcgc cctcgagcag ggccgcgtcg acgaggccat cgagcactac caggccgccc
      181 tgaagctgaa cgaggagttc gccaacgccc atcacaacct cggcgtcgcc taccgccgca
      241 aggggcacct gaacaagagc gtgaagtcgc tgcgcagggc gcagcggagc cagcagcgcc
      301 acgacgccgc ggtggcccgc gagagcctag gcaagtggac gggcaagtcg ggcggcaagt
      361 acctgaagtg gctcttgtgg ggcgcggccg ccatcggcgc ctacttcatc ctgaagtcga
      421 tgggctacat ctgagcctca gctgggctac atgtgaggct cagtcgtcga gcagtcctct
      481 gaggacttcc tcgatggtcg cgtcgtcaag ctcgaggagc cgctcgccgt cgtccgcgtc
      541 gtcgagcagc cgttcgatgc ggcccagcag gacggcgtct tcgcagccgg ccacccgctc
      601 gactatcgcc tcccgtatgt cagccagttc cctcacgcgc gccgcctcgc cttggatggt
      661 aaacccgtaa ggcacgcgac cgaccgtcaa acgcacaccg ccgctttact cgacggtgac
      721 gctcttggcg aggttgcgcg gctggtcgat gtcgcgacca agcgcgcgcg ccacctcgta
      781 cgagaggagc tgcagcggca ccacgttcag gatgggcgac aggagttcga gcgtgcgcgg
      841 caccgggatg acgaactcgg cgtgctgccc gatcttggcg tcgtcctcgt cggccaccac
      901 gatcaggcgg ccttcgcgcg cgcgcacttc ctgcaggttg gagacggtct tgtcgtacag
      961 gggcgaggca gtggcgaccg ccacgacggg taggtcgcga tcgacgagcg cgatggggcc
     1021 atgcttcatc tcgcccatcg ggtacgcctc ggcgtgcacg taggagatct ccttcagctt
     1081 cagcgcgccc tcgagggcgg tggcggcgtt gatgccgcgg ccgaggaaga gggagctgcg
     1141 cgcgtccttg atctgctcgg cgatctgcgc cacgtggtcg cggacctgga gcgtgcgctc
     1201 gacctggccg ggcagttcgc gcagcgcgcg cacgtattcg cgggcggcgt cgtcgtccag
     1261 gtacccgcgc tcgcgggcga accaaaacga gaggagcgcg aaggcggcca ccatgccgag
     1321 gtaggccttc gtgctcgcga ccccgatctc cgggccagcg tggatgtaca ggacgtcgtc
     1381 ggcctcgcgg ctgatgctcg accccttggc gttgaggatg gcgagggtgc gggcgccgcg
     1441 gcgcttggcc tcgcgcatgg cttcgagcgt gtcgatggtc tcgcccgact gggagacgac
     1501 gacgcagagc gtgcgctcgt tgaccaccgg ctcggagtag cggaactcgg aggcgacctc
     1561 gacgacggtg tcgaggcgcg ccatgcgcct gagcagcgtg cggccgacct cgccggcgta
     1621 cgaggcggtg ccggcggccg tgatcacgac gcggtcgatg gcgtgcgggt cgaggttcag
     1681 accgagctcg acgtcgagac cgccgtgcgt gaaccgcccg aagagcgtgt tctgcagcac
     1741 ggtcggctgc tcgtagatct ccttgagcat gtagtgctcg tagccgccct tctcggcggc
     1801 ctccgcgtcc cactcgacgc ggttcgtctc gcgcgcttgt ctgacgccct ggaggtcggt
     1861 gatgaccacg ccgtcgcggc ccagctcggc cacgtcgccg tctaggaggt agatgacgtc
     1921 gcgcgtgtac ggcaggaggg ccggcacgtc ggaagccagg tagttctcgt cggttcccag
     1981 gccgatgacc agcgggctcg tgttgcgcgc ggcgacgagg aggtcgtgct cggcgtgggc
     2041 gacgacgagc gcgtaggcgc cggcgacgcg
//
LOCUS       DAOQYE010000367         2068 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_62451_length_2068_cov_5.985097, whole genome shotgun sequence.
ACCESSION   DAOQYE010000367 DAOQYE010000000
VERSION     DAOQYE010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62451_length_2068_cov_5.985097"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..882)
                     /locus_tag="PKN52_06640"
     CDS             complement(<1..882)
                     /locus_tag="PKN52_06640"
                     /inference="COORDINATES: protein motif:HMM:NF039819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HNQ99660.1"
                     /translation="MAKANKIDSNVTGLRYAEEVSLGVLPASPVWKPLEPNSYNDFGG
                     QITTVARNPINPSRQRKKGVVTDLDASGGFNSDLTQENMQDILQGFFFASLRRKGEME
                     YFTFSKNFVATPATDQLAITSHGLKTGQGPLQASSTTTLPAGLAAATDYWPIVVDDDN
                     IKLATSYANAIAGTAIDITDAGTGTHTLASVAGVTAVDDSFEVSDPSKFKANSLVRST
                     GFATEANNGIHLVSAVTGNLVAVGTALTDEVGPAGAKLVVIGHQGASGDLSIDASGSF
                     PALVSVALDFTTLGLIPG"
     gene            complement(1006..1455)
                     /locus_tag="PKN52_06645"
     CDS             complement(1006..1455)
                     /locus_tag="PKN52_06645"
                     /inference="COORDINATES: protein motif:HMM:NF024943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail terminator-like protein"
                     /protein_id="HNQ99661.1"
                     /translation="MTATPEQAENEILDAFKARWDADGPDAAGLLTAPRVEYDNVVKL
                     APPASGSECWVRATVQHNGEGGHTLGEPGTRLWTSTGIVMIQVFVPQDEGKVRALRLC
                     KVARDAFQGAATPSGVWFRNVRIVHVGRSDPWYQMNVVAEFQYDERR"
     gene            complement(1459..>2068)
                     /locus_tag="PKN52_06650"
     CDS             complement(1459..>2068)
                     /locus_tag="PKN52_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99662.1"
                     /translation="VEDSLPISWQIEDFVTLSAEDAFQMSWQILEHVSDSQPISWRID
                     GYAQAEQEISWPINTYVTATVELSWSIIVSLTVPEDEYTNASDLAARLIAKKGRPLIL
                     RRREDAVPSDPARPWAVDRGGVHDHQMYGVVLDYHKKNIDGELIKRGDKRVLVAASEL
                     VIEPAPRDLVFERDGSDEWTVVSVEVLRPGETSILYTLQIRR"
BASE COUNT          306 a          674 c          726 g          362 t
ORIGIN      
        1 gccggggatc aggccgaggg tggtaaagtc cagggcgacc gacaccagcg cggggaagct
       61 gcccgaggcg tcgatcgaga ggtcgcccga ggcgccctgg tggccgatca cgaccagctt
      121 ggcgcccgcc gggccgacct cgtccgtcag cgccgtgccg acggcgacga ggttgccagt
      181 gacagccgag accaggtgga tgccgttgtt cgcctccgtg gcgaagccgg tcgagcggac
      241 cagcgagttg gccttgaact tggacgggtc agagacctcg aagctgtcgt cgacggcggt
      301 cacaccggcg acagaggcca gcgtgtgcgt gccggtgccc gcgtcggtga tgtcaatcgc
      361 ggtgccagcg atcgcgttgg cgtacgaggt cgccagcttg atgttgtcgt cgtcgaccac
      421 gatcggccag tagtccgtcg cagcggcgag gccggcgggc agcgtggtcg tgctggaggc
      481 ctgcagcggc ccctgccccg tcttgaggcc gtggctggtg atggccagct ggtcagtggc
      541 cggggtcgcg acgaagttct tgctgaaggt gaagtactcc atctcgccct tgcggcggag
      601 ggaggcgaag aagaagccct gcaggatgtc ctgcatgttc tcctgggtga ggtccgagtt
      661 gaacccaccg ctcgcgtcga ggtcggtgac gacgcccttc ttgcgctggc gggaggggtt
      721 gatcgggttg cgagcgaccg tggtgatctg gccgccgaag tcgttgtagc tgttcggctc
      781 cagcggcttc cacacagggg aggccgggag cacaccgagg ctcacctcct cggcgtagcg
      841 gaggccggtt acgttgctgt caatcttgtt ggccttggcc atcgtctgtc tccttgcgca
      901 atcggcctcg cgccgccctg cgaggacggc atcgacgcgc catcttacct gtcgtgtctc
      961 tcatgggcaa ccctggagag gggccggaag agagacttgg agaggctacc gccgctcgtc
     1021 gtactggaac tccgccacca cgttcatctg gtaccagggg tcgctccggc cgacgtgcac
     1081 gatccggacg ttcctgaacc acaccccgct gggcgtagcc gctccctgga aggcgtcccg
     1141 tgcgaccttg cacagcctca gcgcgcggac cttgccctcg tcctgcggca cgaagacctg
     1201 gatcatgaca atgccggtgg aggtccagag ccgggtgccc ggctcgccga gggtatggcc
     1261 gccctcgccg ttgtgctgca ccgtcgcacg cacccagcac tcggagccgg aggccggcgg
     1321 ggccagcttg acaacgttgt catactcgac gcggggcgcc gtgagcagcc cggcggcgtc
     1381 cgggccgtcg gcgtcccacc gcgccttgaa ggcgtcgagg atctcgttct cagcctgctc
     1441 aggggtcgcg gtcatggctc accgacggat ctgcagggtg taaaggatgc tggtctcacc
     1501 gggccgcagg acctcgacgc tcacgaccgt ccactcgtcg gagccgtcgc gctcgaacac
     1561 caggtccctg ggcgccggct cgatgaccag ctccgaggcc gcgaccagca cccgcttgtc
     1621 gccgcgcttg atcagctcgc cgtcgatgtt cttcttgtgg tagtccagca ccaccccgta
     1681 catctggtgg tcgtgcaccc cgccccggtc cactgcccag gggcgagcag ggtcggaggg
     1741 caccgcgtcc tcgcgacggc gcaggatgag cggccggccc ttcttggcga tgagccgggc
     1801 ggcgagatcg ctcgcgttgg tgtactcgtc ctccggcacg gtaagagaga cgatgatgga
     1861 ccagctcagc tccaccgtcg cggtgacgta ggtgttgatc ggccagctga tctcctgctc
     1921 cgcctgggcg tagccgtcga tgcgccagct gatgggctga ctgtcgctga catgctccag
     1981 gatctgccag gacatctgga aggcgtcctc ggcggagagc gtgacgaagt cctcgatctg
     2041 ccagctgatc ggcaggctgt cctcgacg
//
LOCUS       DAOQYE010000368         2068 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_62489_length_2068_cov_2.115748, whole genome shotgun sequence.
ACCESSION   DAOQYE010000368 DAOQYE010000000
VERSION     DAOQYE010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62489_length_2068_cov_2.115748"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(218..634)
                     /locus_tag="PKN52_06655"
                     /pseudo
     CDS             complement(218..634)
                     /locus_tag="PKN52_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002525071.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
     gene            1247..>2068
                     /locus_tag="PKN52_06660"
     CDS             1247..>2068
                     /locus_tag="PKN52_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HNQ99663.1"
                     /translation="MHFNDLLRTVLERGASDLHLHVGLPAMARLDGKLVPITEAQLSP
                     KVTAALVDAMCNEAQKATFATKHQVDLAYSVAGLGRFRVNLFRQRGSVSAVLRVINSD
                     EKALSVVNLPRETIEHFRDQEKGLVLVTGPTGSGKSTTLARIIDEINSAHPKMIVTVE
                     DPIEYLHRSKRSVVVQRELGMDAPSFSEALTAAMRQDPDVILIGEIRDHATAAAAISA
                     ALTGHLVFSTLHTQDSVRTINRMIELFPPHERETARILFAESLVGIVSQRLLGRKG"
BASE COUNT          329 a          727 c          694 g          318 t
ORIGIN      
        1 cgaccgccgt ctggcccgcg agggcgaagg tctggcccgc gagcggcatc gcgccgagga
       61 gcgcctcgat cgcatccgag gccttctcgt catcggcgcc cgggatgtcg atcccctggg
      121 cgcgcaactg cgccacggtg tcgtcgaaga acgcgatggc cgacggcgtg ccagggtcaa
      181 tcctcgtgat ctcgtccaag gcgccttcgc cggctgtgac gcggccgaag atcgtgtggt
      241 tgccgtcgag ccacggcgtc ggctcgaagg tgatgaagaa ctgcgagccg ttcgtgccgt
      301 tcccctgggc gtccttaccg gcgttggcca tcgcgaggac gccgcgcgag tcgaagctga
      361 gctccggtac gatctcgtcg tcgaaagcgt agccggggcc accgcggccg gtgccggtcg
      421 ggtcgccggt ctgcgccatg aagccctcca ggacgcggtg gaacggcacc ccgtcgtagt
      481 aacggttgag cgcgaggaag acgaagttgt tgacggtctc gggggtggcg tcctcgaaga
      541 ggtcgaccgt gatgtcgcca cggctcgtgc gcagcacggc cacgtagtcg aggctcgggt
      601 caaggacctg tcggggtgcg tcgaagacct gcaccggcgc ctcgctcaag tagtcgacct
      661 gcgtgtagcc gtccggcagc gtcacgggag aggtgagccc atcggcttcc ccgacagtcg
      721 gggcgccgga ctcgccggct tcggcatccg tcgtagcgga gccgggagac cccgtgtcgt
      781 cggcctcgac gacgccgggc tcggcctctt ccgcccgcga gccggccggc tgcccgtcag
      841 cgacctggga ggcgtcgact gtcccggtgg gttcgtcgga gctttcctcc tcggcgcttt
      901 cgtgcttggc gctttccgcc ccggcggcag aggtcgcccc cgcggccgcc ggggcgggtg
      961 acaccgcgga cgccgccgag ccgccgatat cgcccggcac agaaccgccc gcggacgaac
     1021 ggtcccttgc cagccagaag ccgctcccga cgatgatgac cgccaggact accagcgcga
     1081 ctacgcgcgt catggccgcc tcatcgcgag gcggccgctt ggggtgatgg gtgctccgct
     1141 catgactggg gcagtgtaac ggttacccgc atgagagcct cgtccgccat cggcggacga
     1201 gcatgcaaga acctcgtaag agcggcatga gtaccgtgtg agacatatgc acttcaacga
     1261 cctgctacgc actgtcctgg aacgaggagc ctccgaccta cacctgcacg tcggcctgcc
     1321 cgccatggcg cgcctcgacg ggaaactcgt gcccataacg gaagcgcaac tcagcccgaa
     1381 ggtgacggcg gcgctcgtcg acgccatgtg caacgaggcg cagaaggcga ccttcgcgac
     1441 caagcatcag gtcgacctcg cgtactccgt cgccggcctc gggcgcttcc gcgtcaacct
     1501 cttcaggcag cgcggcagcg tcagcgccgt cttgcgcgtc atcaactccg acgagaaggc
     1561 cctgtcggtc gtgaacctgc cccgtgagac catcgagcac ttccgggacc aggagaaagg
     1621 gctcgtcctc gtcaccggcc cgacgggctc gggcaagtcg acgaccctgg cccggatcat
     1681 cgacgagatc aactcggcgc atccgaagat gatcgtcacg gtcgaagacc cgatcgagta
     1741 cctgcaccgc tcgaagcggt cggtggtagt gcagcgcgag ctcggcatgg acgcaccgtc
     1801 cttctccgag gctctcaccg ccgccatgcg ccaggacccc gacgtcatcc tcatcgggga
     1861 gatccgggac cacgcgacgg cggccgcggc catcagtgcc gcgctgaccg gccacctcgt
     1921 cttcagcacc ctgcacaccc aggactcggt gcgcacgatc aaccggatga tcgagctctt
     1981 cccgccgcac gagcgcgaga ccgcgcgcat cctgttcgcc gagtcgctcg tcgggatcgt
     2041 ctcgcaacgc ctccttgggc gcaagggc
//
LOCUS       DAOQYE010000369         2066 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_62565_length_2066_cov_3.265042, whole genome shotgun sequence.
ACCESSION   DAOQYE010000369 DAOQYE010000000
VERSION     DAOQYE010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62565_length_2066_cov_3.265042"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..589
                     /locus_tag="PKN52_06665"
     CDS             <1..589
                     /locus_tag="PKN52_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99664.1"
                     /translation="AYIDSLLGAQRGARARNILYWQCSLRPLPDVLVAVGRTRLLDLG
                     VVENDGTKRRYAGYLVDALVQVVQRLQHDPDAKTAAVLPEDAEGDHRATRQRYAFELL
                     QAGRDILPEHLDCAFHAARFLALMALGTPVDGLDTRSTAEGAVLMSKWALRLLERFEN
                     REAGVYVTDEDAMSILAPGYVEELHRLLARLQAPE"
     gene            703..1419
                     /locus_tag="PKN52_06670"
     CDS             703..1419
                     /locus_tag="PKN52_06670"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HNQ99665.1"
                     /translation="MKALYGKTVLDRLEERVAPSRAALIVVDMQNDFCSAGGMMDRLG
                     TNVGLVQATVPVLAELLAAARAAGVLVVYLQNRTRSDGRYSAPADLLRKYLTYADEDD
                     LLITLEGSWGERVIAELEPERGDVVIVKHRPDGFERTQLGLILKSNGIESVLITGTST
                     FACVESTARRALMEDYYVTVVEDCVAATDSDLQAASLTVMRAFFGTECVVPARVLAEV
                     WAAAKAGVPEELVGATGGAD"
     gene            1623..1976
                     /locus_tag="PKN52_06675"
     CDS             1623..1976
                     /locus_tag="PKN52_06675"
                     /inference="COORDINATES: protein motif:HMM:NF025120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNQ99666.1"
                     /translation="MYEAVPLKAELDDLLRDSEELRRRVAVKRDFGIGRSLRYAREQA
                     GLAQSAVAETMGIEQSRVSQIEGAEGHVISLGVLARYSKAVGCRVRIDLLDAESRKVV
                     TRVLVSAEDETAPSP"
BASE COUNT          370 a          632 c          722 g          342 t
ORIGIN      
        1 tgcctacatc gacagcctgc tcggcgcaca gcggggcgcg cgggcgagga atatcctcta
       61 ctggcagtgc tcgctgcggc ccctgccgga cgtcctggtc gctgtgggtc ggacgcgcct
      121 gctggacctc ggcgtggtcg agaacgacgg caccaagcgc cggtacgccg gttacctggt
      181 cgatgcgctc gtccaggtcg tccagcgctt gcagcatgac ccggatgcga agaccgccgc
      241 cgtgttgccc gaggacgccg agggggacca tcgggcgacg cgccagaggt acgcgttcga
      301 actgctgcag gccggacggg acatcctgcc ggaacacctc gactgcgcct ttcacgccgc
      361 gcgctttctc gctctcatgg ccctaggcac tccagtcgat gggctcgaca ctcgaagcac
      421 cgcagaaggg gccgtcctga tgagcaagtg ggcgctgagg ttgctcgagc ggttcgagaa
      481 cagggaggcc ggcgtttacg tgaccgatga ggatgcgatg tcgatccttg cccccggcta
      541 tgtcgaagag ctgcacaggc tcttggcgag gttgcaggcg ccggagtaag cagggccatg
      601 gggcagtcgc gttccgccca cacccttagc caggacgcga acggggttcg atgtaaagtc
      661 cgctatgcgt ctggtaaccg cccggtgccc cgctggaggc ccatgaaagc tctgtacggc
      721 aagacagtgc tcgaccgact cgaggagcgc gtggcgccgt ctcgcgcggc gctgatagtc
      781 gtcgacatgc agaacgactt ctgcagtgcc ggcgggatga tggaccgcct tggcaccaac
      841 gtcggactcg tgcaggccac cgtgcccgtc ctcgccgagc tgctcgcggc cgcgcgggcg
      901 gccggggtgc tcgtcgtcta cctccagaac cgcacgcgct ccgacgggcg ctacagcgcc
      961 ccggccgacc tcctgcgcaa gtacctgacg tacgccgacg aggacgacct cctcatcacg
     1021 ctggaagggt catggggcga gcgggtcata gcggagctcg aaccggagcg gggcgacgtc
     1081 gtcatcgtaa agcaccgtcc ggacggcttc gagcgcacgc agttgggcct gatcctgaag
     1141 agcaacggca tcgagagcgt gctcataacg ggcacctcga cgttcgcctg cgtcgagagc
     1201 accgcgcgcc gtgccctgat ggaggactac tacgtgaccg tcgtcgagga ctgcgtggcc
     1261 gccacggaca gcgacctgca ggccgcgtcg ctgacggtca tgcgcgcgtt cttcggcacg
     1321 gagtgtgtcg ttccagcgcg ggtgcttgcg gaggtgtggg cggcggcgaa ggccggcgtg
     1381 ccggaggagc tcgtgggagc aaccggcggg gcggattgag acccatagaa acggtgttct
     1441 cgagctgcgc ggagagtacg accataggga gctgaggttc tattacttct ggtgccgcag
     1501 ggggctactg gcgagcgctg aactcaagaa gggcaaagca gatcagggca taatcaaggc
     1561 cgccggcaag gctggaaagg ctctcttcaa gggaggaagc gctaatggac gaaagtcagc
     1621 ccatgtacga agccgttccg ctcaaggctg aactggacga ccttctcagg gacagtgagg
     1681 agttgcgacg gcgcgtagct gttaagcgcg actttgggat cgggcgctca ctccgatacg
     1741 cccgagagca ggcgggcctg gctcagagcg cggttgcgga gaccatgggg atcgagcaat
     1801 cgcgagtatc ccagatcgaa ggtgcggagg gtcatgtcat ctcactgggt gtgctggccc
     1861 gctattccaa ggcagtcggt tgccgtgtgc gtatcgacct gctggatgca gagtcgcgga
     1921 aggtggtcac tcgcgttctc gtctctgcgg aagacgagac agctccgagc ccttagcgcg
     1981 agtgcgctaa cgaccgccgc tcgacctcct cgacgctgaa gaacgtcatc ggcataacag
     2041 gcacgttgga gatgagcccg tccgtc
//
LOCUS       DAOQYE010000370         2066 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_62574_length_2066_cov_2.647439, whole genome shotgun sequence.
ACCESSION   DAOQYE010000370 DAOQYE010000000
VERSION     DAOQYE010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62574_length_2066_cov_2.647439"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="PKN52_06680"
     CDS             complement(<1..224)
                     /locus_tag="PKN52_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HNQ99667.1"
                     /translation="MTPASQLDELVFGTDGWRDVIAERFTAANLARAAQAYAEHLIED
                     GVGAVLVGYDTRFMGRRFARLVAEVLTGNG"
     gene            443..1222
                     /gene="argB"
                     /locus_tag="PKN52_06685"
     CDS             443..1222
                     /gene="argB"
                     /locus_tag="PKN52_06685"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HNQ99668.1"
                     /translation="MSIVIKYGGNAMTDQATRRAVAAGLRNLAGSNGAGASDAVPPVV
                     VHGGGPYIQAALDEAGLEHRFERGLRVTSPESMAIIERVLTMLSKELAYEIGPAVGVT
                     ARDARTVTAEVRDPALGRVGRVTHVDTRVIRALRDAGLIPVLACLAVDIDGGALNVNG
                     DEAAGAVAGALGEGVVFLTNVPGVLDDPAVPGSLLDSLTRTDALARIADGRIAGGMIP
                     KVEAALDALAAGAPFAVVADGRDPHGVEAALAGGGTRILAG"
     gene            1357..1533
                     /locus_tag="PKN52_06690"
     CDS             1357..1533
                     /locus_tag="PKN52_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99669.1"
                     /translation="MHDDITTLPTPDLIRQATVLELKIIHDTSSAMETERYCLIQVEL
                     GGRLARDLVVDPRD"
     gene            1711..>2066
                     /locus_tag="PKN52_06695"
     CDS             1711..>2066
                     /locus_tag="PKN52_06695"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNQ99670.1"
                     /translation="MRSSSLFRSVLAVVVLGTAAASAKTITVQPGDSLWALARRFDTT
                     VAELRELNGLSSDALRPGQVLTVPGDEEEAAAPATVVVVRAGDTLYDIALANNVSVAD
                     LIAFNDLDGTLIHPGQ"
BASE COUNT          303 a          762 c          699 g          302 t
ORIGIN      
        1 ataccgttgc cggtcagtac ctccgcgaca aggcgcgcga agcgcctacc catgaagcgc
       61 gtgtcgtagc cgacgagcac ggcccccacg ccgtcttcga tgaggtgctc ggcgtaggcc
      121 tgcgccgcgc gggccaggtt ggcggcggtg aaccgctcgg cgatgacgtc gcgccagccg
      181 tcggtaccga acacgagctc gtcgagttgg cttgctggtg tcacctagcc tccactcaca
      241 ccgccaagcg accgcggtgc gtcccgtccc ccacccgccc gcggcgctcc gagatgccgg
      301 agggtaccac ccggcgccct tggcccgatc cgtccgcgct cctgcggacg cggggcccca
      361 cgcacaagtc gctggccggc acgtcccgat ccgtccgcgc tcctgcggac gcgggagcgc
      421 gcgggaccgt agacttgcgc tcgtgagcat cgtcatcaag tacggcggca acgccatgac
      481 cgaccaggcc acgcgccgcg ccgtggccgc cggcctgcgc aacctcgccg gcagcaacgg
      541 agcgggcgcc tccgacgcgg ttccgcccgt ggtggtgcac ggcggcgggc cgtacatcca
      601 ggccgccctc gacgaggcgg gtctggagca ccgcttcgag cgcgggctgc gcgtcaccag
      661 cccggagtcc atggcgataa tcgagcgcgt ccttaccatg ctctcgaagg agctggcgta
      721 cgagatcggc ccggcggtag gcgtcacggc gcgcgacgcg cgcaccgtca ccgccgaggt
      781 ccgcgacccg gcgctcgggc gggtcggacg cgtcacccac gtcgataccc gcgtgatacg
      841 cgctctgcgc gacgccggtc tcatccccgt cctggcctgc ctggccgtcg acatcgacgg
      901 cggtgcgctc aacgtgaacg gcgacgaggc cgccggggcg gtcgcggggg cgctcggcga
      961 gggcgtcgtg ttcctcacca acgtgcccgg ggtcctcgac gacccggccg tgccgggttc
     1021 gctgctcgac agcctcacgc gcaccgacgc gctggcgcgc atcgcggacg gccgcatcgc
     1081 cgggggcatg atccccaagg tcgaagcggc cctcgacgcg ctcgcggccg gggcgccgtt
     1141 cgccgtcgtc gccgacggtc gcgacccgca cggggtcgag gcagcgctgg ccggcggcgg
     1201 gacgcgcatc ctggctggtt gaagcggcgc ctcgcgggtc gaggccgcgc ggtgagattc
     1261 ctgtaagaga cccgaagcta ccttggtgtc ggcaccgccc tgcaggagtt cggggcggca
     1321 ccgtggagac gagctagccg ctctaggagc ctcaagatgc atgacgacat cacaacgctg
     1381 cccacaccgg atctgatccg ccaggccacc gtgctcgagc tgaagatcat ccacgacacc
     1441 agctcggcca tggagaccga gcggtactgc ctgatccaag tggaactcgg cggtcgtctc
     1501 gcccgagacc tggtggtcga tccacgggac tgacgcgatg ggcacccgct ccctccctta
     1561 gcaacggccc ggcgggtgcc gtcgtttaag cgcatgcaac gccccggaac ggggtctggc
     1621 acggcatagc cgacgacgcc tcacgttccg cgatgcgatt gccgggtttt tcctgttgcg
     1681 ggtagcgctc gttctggtac atttgacaac gtgcgctcat ccagcctgtt ccgttccgtc
     1741 ctcgcggtgg tcgtcctggg gaccgcagcg gcatccgcca agaccatcac ggtgcaaccg
     1801 ggcgacagcc tctgggcgct agcacgccgt ttcgatacaa ccgtagccga gctccgcgaa
     1861 ctcaacgggc tctcctccga cgcgctccgc cccggtcagg tcctcaccgt gccgggcgat
     1921 gaagaggagg ctgccgcgcc ggccacggtc gtggtcgtgc gcgccggcga cacgctttac
     1981 gacatcgcgc tggcgaacaa cgtgtctgtc gccgacctca tcgcgttcaa cgacctcgac
     2041 ggcacgctca tccaccccgg ccaaga
//
LOCUS       DAOQYE010000371         2066 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_62575_length_2066_cov_2.601691, whole genome shotgun sequence.
ACCESSION   DAOQYE010000371 DAOQYE010000000
VERSION     DAOQYE010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62575_length_2066_cov_2.601691"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..709
                     /gene="sppA"
                     /locus_tag="PKN52_06700"
     CDS             <1..709
                     /gene="sppA"
                     /locus_tag="PKN52_06700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HNQ99671.1"
                     /translation="LRAAGRDKSTKAVVFHVESGGGSALASDLIGREVELLAKRMPVV
                     AVMGGVAGSGGYYVLTHATRVVAQPTTLTGSIGVVTLKPVLKELYDRYGVNVESLERG
                     RFAEVMASHKPFSPGDRELLERYIGEVYERFVMRVAAGRGLSSERVNEIGRGRIWSGA
                     DAKELGLVDELGGLGLGLRLAKELAGLPHDAGQWRVPAPTKYLLPTADDPTTWLRLLG
                     ANLREAAWLLGEPVTVR"
     gene            784..1536
                     /locus_tag="PKN52_06705"
     CDS             784..1536
                     /locus_tag="PKN52_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNQ99672.1"
                     /translation="MTGKVALVTGAGSGIGRATALEFAARGAAVMVADFNLAGAEETV
                     RQVLANGGRAVASKVDVADEASVKGMVASTVEAFGRVDYLVNNAGIAAAGPNVPLHEV
                     DVEAFERVQAVNVVGTFLGLKYAIPELLKMGGGGIVNLTSTMGDRATAGDPSYPTSKH
                     AVRGLTKSAALTYATQGVRVNCVGPGVVRTGMTEAIFEDEQTTAWLKSVTPMRRFGEP
                     SEVAKLIVFLCSEDASYITGAYYPIDGGWLAG"
     gene            complement(1667..>2066)
                     /locus_tag="PKN52_06710"
     CDS             complement(1667..>2066)
                     /locus_tag="PKN52_06710"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNQ99673.1"
                     /translation="LPAVALESLAGEVVGLSPGQPLVVNEWATWCLPCRRELPQLARA
                     AEEAPGVKFLLVNQGEGRAVVEAFLADYPAVRRGDVLLDRGGVLAAELRGVGLPTTYF
                     FSAAGTLLGTHVGEISGAALERWVRRLQDD"
BASE COUNT          330 a          705 c          744 g          287 t
ORIGIN      
        1 gctgcgcgcc gccggccgcg acaagagcac aaaggccgtc gtgttccacg tcgagtcggg
       61 cggcgggtcg gcgctcgcct ccgacctcat cggacgcgag gtggagctgc tcgcgaagcg
      121 catgcccgtg gtcgccgtca tgggcggcgt ggcggggagc ggcggctact acgtcctcac
      181 ccatgccacg cgtgtcgtcg ctcagccgac gacgctgacg gggtcgatcg gcgtcgtcac
      241 gctcaagccc gtgctgaagg agctgtacga tcgctacggc gtgaacgtcg agagcctcga
      301 gcgcgggcgc ttcgcagaag tcatggcctc tcacaagccg ttctcccccg gcgaccggga
      361 gctgctcgag cgttacatcg gcgaggtgta cgagcgcttc gtcatgcgcg tggccgccgg
      421 acgcgggctg agctcggaac gggtgaacga gatcggccgc ggccgcatct ggtccggggc
      481 ggacgccaag gagctcggtc tcgtcgacga actcggcggc ctggggctcg ggctgcgcct
      541 ggccaaggag ctagcgggcc tcccgcatga cgccgggcag tggcgcgtgc ccgcgcccac
      601 caagtacctc ctcccgacgg cagacgaccc gaccacctgg ctccgcctgc tcggcgcgaa
      661 cctacgcgag gccgcctggc tgttgggtga gcccgtaacc gtgcgctgac agaccgagcg
      721 cggccatttg tcctacgcac cccctcttac gctgtaagag aaaagaggtg ccgcatggac
      781 acgatgacag gcaaagttgc gctggtgacc ggtgcgggtt ccggcatcgg gcgcgctacc
      841 gcgctcgagt tcgctgcgcg aggcgcggcc gtgatggtcg ccgacttcaa cctggccggc
      901 gcggaagaga cggtacgcca ggtgcttgcg aacgggggca gggcggtggc cagcaaggtc
      961 gacgtcgccg acgaggcgtc tgtcaagggg atggtggcca gcacggtgga ggcgttcggg
     1021 cgcgtcgact acctcgtcaa caacgccggc atcgccgccg cagggccgaa cgtgccgctg
     1081 cacgaggtcg acgtcgaggc gttcgagcgg gtgcaggccg tgaacgtggt cggcacgttc
     1141 ctgggtctga agtacgcgat ccccgaactc ctcaagatgg gcggcggcgg catcgtcaac
     1201 ctgacgagca ccatgggcga ccgcgccacg gccggcgacc cgtcctatcc aacgagcaag
     1261 cacgcggtgc gcggcctgac caagtccgcg gccctcacct atgcgaccca gggcgtgcgc
     1321 gtgaactgcg tcggcccagg cgtcgtgcgc acgggcatga cggaagccat cttcgaagac
     1381 gagcagacca cggcgtggct gaagagcgtg acgcccatgc ggcgcttcgg cgagccgtcc
     1441 gaggtcgcca agctcatcgt cttcctgtgc tccgaggacg cctcgtacat caccggcgcg
     1501 tactacccca tcgacggcgg ttggctggcg gggtgaccta gcagtggctt gtggcgtcgt
     1561 cccggggtgc ctggggcgac gcccggacga ccaaggcgac gagacatcca gaccggtccg
     1621 ggtcccgcag ggagcgagct cggcggggcc tcgatcgacg cgcccatcag tcgtcctgga
     1681 gccgccgcac ccagcgctcc agcgctgcgc cggagatctc gcctacgtgg gtgccaagca
     1741 aggtcccggc agcggagaag aagtacgtgg ttgggagccc gacgccgcgc agttcggctg
     1801 cgaggacacc gccgcgatcc aggagcacgt cgccgcgcct cacggccggg tagtcggcca
     1861 ggaacgcctc gacgacggca cggccctcgc cttggttgac gagcaggaac ttgacgccgg
     1921 gggcctcttc ggcggcgcgg gccaactgag ggagctcgcg ccgacacggt aggcaccacg
     1981 tggcccactc gttgacgacc aacggctgcc ccggggacaa acctacgacc tcgccggcga
     2041 ggctctccaa cgccaccgct gggagc
//
LOCUS       DAOQYE010000372         2062 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_62767_length_2062_cov_2.777778, whole genome shotgun sequence.
ACCESSION   DAOQYE010000372 DAOQYE010000000
VERSION     DAOQYE010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62767_length_2062_cov_2.777778"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..774)
                     /locus_tag="PKN52_06715"
     CDS             complement(<1..774)
                     /locus_tag="PKN52_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99674.1"
                     /translation="MFRVGAALLACALLGGTALAQVSYRLGGLVTADFGVTVDGTIPV
                     AVAGLTLKVDGEVGSGLFPDAVFVTELHTSYDAAAEERFAVGLGRAYATVYLGPVDLS
                     VGNQVVAWGSTDAVNPVDVVNPRDLSNPVADPADQRLPTPLLRATVHAQEGLTVDMVV
                     VPVFVPSTLPGERWQPAAELPTLPPGTALVGVLPPTDATPAFELKNVQFGARATLDLD
                     LGGGADVSAVVYRGFRHLPTVTAELEPTGYWPHHAVSHVL"
     gene            complement(984..1784)
                     /locus_tag="PKN52_06720"
     CDS             complement(984..1784)
                     /locus_tag="PKN52_06720"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HNQ99675.1"
                     /translation="MTAHQNDRRAPTHRPPRSALIVLVVVALAGVAFAQSYTTAKEVV
                     DAMEAKPAPSTTIATMSMTITAASGHSLTRTMQIWSADEGRSQLIKFLEPADVRGSGF
                     LSVEKASGETETMIYLPALGRVRRVAGGQKQDAFFGSDFSYEEISSLSGDFGDDLETT
                     LLETLPGPVYVIEGVAKPGSDASYDRVVFQVPEATLLPQRVEFYRSGELVKVLTISGA
                     ATVGGYDLPSVIRMETVATGSYTTIDQSEFEVDEAIPADVFTERFLQR"
     gene            complement(1827..>2062)
                     /locus_tag="PKN52_06725"
     CDS             complement(1827..>2062)
                     /locus_tag="PKN52_06725"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HNQ99676.1"
                     /translation="HALHSSGRSILFTALTLGAGFAAMIFSQFVPVRTLGTLMAFTVV
                     SVGTASLTLLPAACLVFLRNPRGSATTATTADA"
BASE COUNT          302 a          671 c          780 g          309 t
ORIGIN      
        1 gaggacgtgg ctgaccgcat ggtgcggcca gtagccggtc ggctccagct cggcggtcac
       61 cgtcggcagg tgcctgaagc cgcggtagac gacggcgctc acgtcggccc cgccacccag
      121 gtcgaggtcg agggtcgcgc gcgcgccgaa ctgcacgttc ttcagctcga aggccggcgt
      181 cgcgtcggtg ggaggcaaga cccctactag cgccgtcccc ggtgggagcg tcggcagctc
      241 ggcggcgggc tgccagcgct cgcccggcag ggtcgacggc acgaagaccg gtaccactac
      301 catgtcgacg gtcaggccct cctgagcatg cacggtggcg cgcagcagcg gcgtcggcag
      361 ccgctggtcc gcggggtccg cgaccgggtt cgagaggtcg cgggggttca cgacgtcgac
      421 ggggttcacg gcgtcggtcg aaccccacgc gacgacctgg ttgccgacgc tcaggtcgac
      481 cggcccaagg tagacggtcg cgtacgcacg gcccaggccg accgcgaacc gctcctcggc
      541 ggccgcgtcg tagctggtgt gtagctcggt cacgaagacg gcgtcgggga agagccccga
      601 gccgacttcg ccgtcgacct tcagggtcag tccggcgacc gccaccggga tggtgccgtc
      661 gacggtcacc ccgaagtccg cggtcaccag cccgccgaga cggtagctca cctgggcgag
      721 cgccgtgcca ccgaggaggg cgcacgccag gagcgcggcg cccacgcgga acaaggaccg
      781 cctggtccgc ccggttcgcg gcgccagcgc cgaccggcca ccggacgcca tccccgccag
      841 ggcggttcgg ttgctttggt gcaacgatct gtctctcacg atgatcacct cttgcccgtc
      901 aggcgcttcc gcagggggct tcggtaccgg actgggcttg ggtaccgcac tgtgcgcttc
      961 gtcaacggac tgcgcgccgg gtctcagcgc tgcaggaagc gttccgtgaa gacgtcggcc
     1021 gggatggcct cgtcgacttc gaactccgac tggtcgatgg tcgtgtacga cccggtcgcg
     1081 accgtctcca tgcggatgac gctcggcagg tcgtacccgc cgaccgtggc cgcgccgctg
     1141 atcgtcagga ccttgacgag ctcgcccgag cggtagaact ccacgcgttg tggtaggagc
     1201 gtggcctcag ggacttggaa gacgacccgg tcgtagctgg cgtcggagcc gggcttggcg
     1261 accccctcga tgacgtacac ggggcccggg agggtctcga ggagggtcgt ctcgaggtcg
     1321 tcgccgaagt cgccgctcag gctcgagatc tcctcgtacg agaagtcgga gccgaagaag
     1381 gcgtcctgct tctgaccgcc ggccacgcgg cgcacgcggc cgagggcggg caggtagatc
     1441 atggtctccg tctcgccgga ggccttctcg acggagagga agccggagcc gcgcacgtct
     1501 gccggctcta ggaacttgat gagctgcgac cgtccctcgt ccgccgacca gatctgcatg
     1561 gtgcgcgtca gggagtggcc ggaggccgcc gttatggtca tgctcatggt cgcgatggtc
     1621 gtgcttggcg ccggcttggc ctccatggcg tcgacgacct ccttggccgt cgtgtagctc
     1681 tgggcgaagg cgacgccggc gagggctacg accacgagga ctatcagcgc ggatcgcggg
     1741 gggcggtgcg ttggggcgcg gcggtcgttc tgatgggcgg tcatggtgtt acctccggtg
     1801 gtgggcatcg cgggtgcgtt catggctcag gcgtcagccg ttgtcgcggt cgtcgcggag
     1861 ccgcgcgggt tgcgaaggaa gacgaggcag gcggccggca gcagggtgag cgaggccgtg
     1921 ccgacggaga cgacggtgaa ggccatgagc gtgccgagcg tgcgcaccgg cacgaactgg
     1981 ctgaagatca tggccgcgaa gccggcgccg agcgtcaggg ccgtgaagag gatcgaacgg
     2041 ccggacgagt gcagggcgtg gc
//
LOCUS       DAOQYE010000373         2055 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_63149_length_2055_cov_3.238000, whole genome shotgun sequence.
ACCESSION   DAOQYE010000373 DAOQYE010000000
VERSION     DAOQYE010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63149_length_2055_cov_3.238000"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(118..1440)
                     /locus_tag="PKN52_06730"
     CDS             complement(118..1440)
                     /locus_tag="PKN52_06730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99677.1"
                     /translation="MFRLGVVGHRDTLQVVEGIVQEYFDDVAVVPEEFGNDDDIVDAV
                     QRISHLQTRCDGILYSRKDPYLLVTSRLHHNVPVRYVEVDSSDLLISLLAAMVQLGSV
                     PDSISVDSFDAPTVAAALGSVGISDGSVAIRCVSPGQGGDGFVKATLQQHVGNHSAGS
                     KLCITNITDVHHSLLGLGVPSTLISPSLESFVHEIRNLMLRHQLRTQDSTGLAIMHIH
                     LQYKEKYRYHGTMPIREVDDLSKAAKLIAVFAEEVDSAMFQLSRWEYLILCSRSLLEN
                     ATDDFADISIMRDINLETTFDAAVSVGCGQTVKQAEANAFSALNEGHTLTGTYALVVT
                     GSGEPLGPIKPKSQRYPHQSGPEANLTRIAGETGVSTQVLNRLYGLTVARNTNLFTSA
                     ELAGLLGVSTRTVNRIVGRLLPHGYAAVEGKDLTHGFGRPARVLRFRL"
     gene            1581..>2055
                     /locus_tag="PKN52_06735"
     CDS             1581..>2055
                     /locus_tag="PKN52_06735"
                     /inference="COORDINATES: protein motif:HMM:NF014476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99678.1"
                     /translation="MREELPVERVSAVPHARSPEYAAGPLVITGDQVAGLLDMGTCIG
                     LMRTALTALGTDQARQMVRPVLPLHGRNVLGMMPAYDQASEVAGVKVLSVFPDNYRSG
                     RPSHQGLILLFETRGGAVKAVVDAEAVTAIRTAAASAAATDALARQHFRTLRELFK"
BASE COUNT          347 a          685 c          669 g          354 t
ORIGIN      
        1 cgcgcagtat ggcagcacct ggctagggac catgcgcgag atcgtcgacg aagaagaact
       61 gtccagcggc cggttcgagc tgcggcgcgc accctgacgc gcccctcacg atgccgctca
      121 caacctgaac cgcaacaccc gagcaggcct gccgaacccg tgggtcaggt ccttgccctc
      181 aacggccgcg taaccgtgcg gcaacaaccg cccgacgatg cggttcacgg tccgcgtgct
      241 cacgcccaac agccccgcca actcggccga cgtgaacagg ttcgtgtttc gcgccaccgt
      301 gagcccgtag aggcggttca ggacctgcgt gctcacgccg gtctcccccg cgatacgcgt
      361 caggttcgct tccgggccgc tctggtgcgg gtaacgttgg ctcttcggtt tgatcggacc
      421 gagcggctcg cccgagccgg tgaccaccag cgcgtaagta cccgtcaacg tatggccctc
      481 gttcagggcg ctgaacgcgt tcgcctctgc ctgcttgact gtctggccgc aaccgacgct
      541 gaccgccgcg tcgaaggtcg tctccaggtt gatgtcgcgc atgatgctta tgtcggcgaa
      601 gtcgtccgtc gcgttctcga gcagcgaccg gctacacagg atcaggtact cccagcggct
      661 cagttggaac atagcgctgt cgacctcctc cgcgaacacg gcgatgagct tggccgcctt
      721 gctgaggtcg tcgacttccc ggatgggcat ggtgccgtgg tagcggtact tctccttgta
      781 ctgcaggtgg atgtgcatga tggccaggcc cgtcgagtcc tgggtgcgga gctggtgccg
      841 cagcatcagg ttccgtatct cgtgaacgaa ggactcgagc gacgggctga tcagggtgct
      901 aggcacccca agcccgagga gcgagtggtg cacgtccgtg atgttcgtga tgcacagctt
      961 gctgccggcc gagtggttgc cgacgtgctg ctgtagggtc gccttcacga acccgtcccc
     1021 gccctgcccc gggctgacac agcggatcgc gacgctaccg tccgagatac cgacgctacc
     1081 gagggccgcg gcgaccgtgg gggcgtcgaa cgagtcgacg ctgatggagt cgggcacgga
     1141 ccccagctgc accatggcgg cgagaaggct gatcaggagg tcggagctat ccacctctac
     1201 gtagcggacg ggcacgttgt gatggaggcg gctcgtcacg agcaggtacg gatccttgcg
     1261 cgagtagagg atcccgtcgc aacgggtctg caggtgggag atgcgctgca ccgcgtcgac
     1321 gatgtcgtcg tcgttgccga actcctcggg aacgacggct acgtcgtcga agtactcctg
     1381 gacgatacct tcgacgacct gcaaggtatc gcggtggccg accacgccaa gcctgaacat
     1441 gcttgcttta cctcgctccc gatcgagccg cccgaagcgc gggaaacgcg caggcacctc
     1501 ggcacgtcac ttatggacga cttagagtca gtatacagac agccgccttc gcgcttaccc
     1561 tccttcgtat aggaggtccg gtgcgcgaag agctaccagt ggaacgggtt agcgcggtgc
     1621 cgcatgccag aagcccggag tacgctgccg ggccgctcgt catcacgggc gaccaggtcg
     1681 cgggcctgct cgacatgggc acctgcatag gcctgatgcg cacggccctg acggcgctcg
     1741 gcaccgacca ggcccgccag atggtacgcc ccgtccttcc cctccacggc cgcaacgtgc
     1801 tggggatgat gcccgcctac gatcaggcct ccgaggtggc gggcgtgaag gtcctgagcg
     1861 tcttccccga caactaccgc agcggcaggc cgtcgcacca ggggctgatc ctcctgttcg
     1921 agacgcgtgg cggagccgtc aaagcggtgg tcgacgccga ggccgtcacc gccatccgca
     1981 ctgcggcggc gtccgcggct gcgaccgacg ccctggccag gcagcacttt cgcactttac
     2041 gcgaactttt caaag
//
LOCUS       DAOQYE010000374         2054 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_63221_length_2054_cov_2.393697, whole genome shotgun sequence.
ACCESSION   DAOQYE010000374 DAOQYE010000000
VERSION     DAOQYE010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63221_length_2054_cov_2.393697"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..672)
                     /locus_tag="PKN52_06740"
     CDS             complement(<1..672)
                     /locus_tag="PKN52_06740"
                     /inference="COORDINATES: protein motif:HMM:NF036997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99679.1"
                     /translation="MPSAIDVLLPLPIGQLTYLPPVAGRSAQDDDAVMPAPGQRVVVP
                     WQAGVRIGIVIDVRTVDAGRGLELRHAVQTLESRPWLTRAGLATTLQLARHSGVPAGL
                     VLATLNPPGLQQELDHQVRVHAGADDYLSAMATPLKQSVTWQEGWSEWLPAAAVPAKG
                     LEELRRQGLVDERVLPVVPTRTVLVPAREPDDELEGARRLAQRIALQHQHAQAAVGLQ
                     AHVGVQ"
     gene            740..>967
                     /locus_tag="PKN52_06745"
     CDS             740..>967
                     /locus_tag="PKN52_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99680.1"
                     /translation="MAAGDLTDDRSGAYKAAGVDLGAADEALARIGAAVASTYTPGVI
                     RGLGAFGGLFALPGGMREPTLVASTDGVGTKT"
     assembly_gap    968..977
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <978..1792
                     /gene="purM"
                     /locus_tag="PKN52_06750"
     CDS             <978..1792
                     /gene="purM"
                     /locus_tag="PKN52_06750"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HNQ99681.1"
                     /translation="RLRQIGRDLVNHCVNDILVQGAEPLFFLDYVASSRLEPDVVAEV
                     VTGVAEACREAGMALLGGETAEMPGVYVPGQLDVAGTIVGIVERAAIVDGSGVAEGDV
                     LLAFESGGLQTNGFSLARHLVLSGGGLAAPLPGDADERTVGAALMAEHRSFLPAVRPL
                     LAAGLVKAMAHITGGGLPGNLPRSLPTGLGAVVRRGSWPVPAVFEHLVALGDVREDDA
                     YSAFNMGVGFVLVVSPTAVKEVGELVALSPAGAWARLHRIGEVVPDSGVRLE"
     gene            complement(1798..>2054)
                     /locus_tag="PKN52_06755"
     CDS             complement(1798..>2054)
                     /locus_tag="PKN52_06755"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNQ99682.1"
                     /translation="HLFERFFRVDGARSTRGNGSGLGLAIVKWIVQQHGGSVDVESRL
                     GEGTVFTVNLPALEVRNTMDLATNVRATLVENVIARVRPQ"
BASE COUNT          266 a          707 c          771 g          300 t
ORIGIN      
        1 ctgcacaccg acatgcgcct gcaggccgac ggcagcctgc gcatgctggt gctggagggc
       61 gatgcgctgg gccaggcgcc tggcgccctc cagctcgtcg tcgggttcgc gcgcggggac
      121 gagcacggtc ctggtcggca ctacgggcag tacgcgctcg tcgaccaggc cctgccgacg
      181 cagctcctcc aggcccttgg caggcacggc ggcggccggc agccactcgc tccagccttc
      241 ctgccaggtc acggactgct tgagcggcgt tgccatggca ctcaagtagt cgtcagcccc
      301 cgcgtggacg cggacctgat gatccagctc ctgctgcagt cccggtgggt tgagggtcgc
      361 gagcacgaga ccggccggca ccccggagtg ccgcgccagc tgcagggtgg tggccaggcc
      421 ggcccgcgtg agccagggtc gggactccag cgtctggacg gcatgcctga gctccaagcc
      481 cctgccggcg tcgacggtcc tgacgtcgat cactatcccg atgcgcacgc cggcctgcca
      541 tggcaccacc acgcgctggc cgggggcggg catcaccgcg tcgtcgtcct gggccgaccg
      601 ccccgcgacc ggcggcaggt aggtcaactg gcctatcggc agggggagga ggacgtcgat
      661 ggctgacggc acttccccaa gtctagccac cgcccgccgc gccggacggc ggcgggcgac
      721 gggcagttac gatgcgccca tggctgcggg agaccttacc gacgatcgga gcggggcgta
      781 caaggccgcc ggggtggacc tgggggcggc cgacgaggcg ctggcgcgca tcggcgccgc
      841 cgtggcgagc acgtacacgc cgggggtgat acgcggactc ggcgccttcg gcgggctgtt
      901 cgccttgccg ggaggcatgc gcgagccgac gctggtcgcc tcgacggacg gcgtcggtac
      961 caagacgnnn nnnnnnngcc ggttgcggca gatcgggcgc gacctcgtca accactgcgt
     1021 caacgacatc ctcgtccagg gggcggagcc gctcttcttc ctcgattacg tggcgtcctc
     1081 gcgtctcgaa cccgacgtcg tggcggaagt cgttaccggc gtggcggaag cgtgccggga
     1141 ggcgggtatg gccctgctcg ggggcgagac ggccgagatg cccggcgtct acgtacccgg
     1201 ccagctagac gtcgcgggga ccatcgtcgg catcgtcgag cgggcggcca tcgtcgacgg
     1261 ctccggcgtc gcggagggcg acgtgctgct cgccttcgag tccggcgggc tgcagacgaa
     1321 cggtttcagc ctggcgcggc acctcgttct ctcggggggc gggctcgcag ctcccctgcc
     1381 gggcgacgcc gacgagcgca ccgtcggcgc cgcgctcatg gcggagcacc gaagcttcct
     1441 gcccgccgtg cgtccgctgc tagccgcggg cctcgtcaag gccatggcgc atatcacggg
     1501 aggcggcctg ccggggaacc tgccacgcag cctgccgacc gggctcgggg cggtcgtgcg
     1561 caggggcagt tggcccgtgc ccgccgtctt cgagcacctg gtcgccttgg gcgacgtccg
     1621 cgaggacgac gcgtactcgg ccttcaacat gggggtcggc ttcgtgctgg tggtctcccc
     1681 gaccgccgtg aaggaggtcg gggagctggt cgccctatcg cccgccgggg cttgggcacg
     1741 gctgcaccgg atcggcgagg tcgtccccga ctccggggtc cgtctcgagt aagcgggcta
     1801 ctggggcctg acgcgcgcga tgacgttctc tacgagcgtg gcgcgcacgt tggtggcgag
     1861 gtccatggtg ttcctgacct cgagtgcggg caggttgacg gtgaagacgg tgccctcgcc
     1921 gaggcgcgac tcgacgtcga cgctgccccc atgctgctgg acgatccact tgacgatggc
     1981 gagcccgagg ccgctgccgt tgccgcgcgt ggagcgcgcg ccgtcaacgc ggaagaagcg
     2041 ctcgaagagg tgcg
//
LOCUS       DAOQYE010000375         2051 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_63335_length_2051_cov_3.299599, whole genome shotgun sequence.
ACCESSION   DAOQYE010000375 DAOQYE010000000
VERSION     DAOQYE010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63335_length_2051_cov_3.299599"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /gene="pgi"
                     /locus_tag="PKN52_06760"
     CDS             complement(<1..235)
                     /gene="pgi"
                     /locus_tag="PKN52_06760"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012562.2"
                     /note="functions in sugar metabolism in glycolysis and the
                     Embden-Meyerhof pathways (EMP) and in gluconeogenesis;
                     catalyzes reversible isomerization of glucose-6-phosphate
                     to fructose-6-phosphate; member of PGI family; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HNQ99683.1"
                     /translation="MRVADLFAGDAGRFERFSFQAGELFVDLSKNLITPETLALLLRL
                     AEASGVEERKRAMFAGEHVNTSEDRAVLHVALRN"
     gene            complement(406..1467)
                     /locus_tag="PKN52_06765"
     CDS             complement(406..1467)
                     /locus_tag="PKN52_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNQ99684.1"
                     /translation="MTDAPAMTYARSGRSGLLLPKVSLGLWHNFGDDRPLASMSAVLQ
                     RAFELGITHFDLANNYGPPAGAAEKNFGEILATDFAGLRNRLVISTKAGYYMWPGPYG
                     EWGSRKYLLSSLEESLDRMRLDWVDIFYSHRYDPDTPLEETMGALTSAVQQGLALYPG
                     ISSYSAATTREAAGILRDSGTPLLIHQPSYSMFNRWIEDGLLDALGEEGVGCIVFSPL
                     AQGLLTSRYLGVDASDVAGTLPPDSRAAEGGSLKPSALTPEKLERIHALAAIAKRRGQ
                     TLAQLALAWCLRDDRVTSVLVGASSVAQLEENVGTLANLEFTAAELAEIDELAVEGNI
                     NIWRKSSEAGGERPAEARN"
     gene            complement(1491..1979)
                     /locus_tag="PKN52_06770"
     CDS             complement(1491..1979)
                     /locus_tag="PKN52_06770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNQ99685.1"
                     /translation="MAEPIDLSPYRTLLRREESYAIHALIYASENPGAAAARIAGDLK
                     IPPAFLAKVLRRLAQVGYVENRQGRNGGVNLIVDPTSISLLDVIETMSGPLIVDTCQT
                     KTRCATQERKGYCRLNVAWVNASLAIREVLSGVRLSHLIDPPRTGEARKPRALRRMSA
                     DA"
BASE COUNT          294 a          672 c          743 g          342 t
ORIGIN      
        1 ggttcctgag cgccacgtgg aggacggcgc ggtcttccga cgtgttcacg tgctcgcccg
       61 cgaacatggc gcgcttgcgc tcctcaaccc cgctggcctc cgccagacgc aggagcagcg
      121 cgagcgtctc gggcgtgatg aggttcttgc tcaggtcgac gaacagctcg ccggcttgga
      181 aggagaagcg ctcgaagcgc ccggcgtccc cggcgaagag atctgcaaca cgcacgttgc
      241 ataaatgttc ctggtgctca cggaggaggc cccaggcagc cgctgcacct acaggcgcac
      301 cggtcgctgc ctttgcgggg gctccggtcg ccgcccttgc aggcgcaccg gtcgctatcc
      361 ttgcgggggc tccggtcgcc gcctgctccg ggatcccggc cacgctcagt tcctggcttc
      421 cgccgggcgt tcccctccgg cctcgctcga cttccgccag atgttgatgt tcccctctac
      481 ggcgagctcg tcgatctccg cgagctcggc cgcggtgaac tcgaggttgg cgagcgtgcc
      541 gacgttctcc tccagctgcg ccacgctgga cgcgccgacc agcacggacg tgacgcggtc
      601 gtcgcgcaag caccacgcca gggcgagctg ggcgagggtc tggccgcgtc tcttcgctat
      661 cgcggccagt gcgtggatgc gctccagctt ctccggggtg agcgcgctgg gcttgaggga
      721 gccgccctcc gccgcccgcg agtcgggtgg cagggtgccg gccacgtcgg acgcgtccac
      781 accgaggtag cgcgaggtga gcagcccctg cgccagcggc gagaacacga tgcagccgac
      841 gccttcctcg ccgagggcgt cgagcagccc gtcctcgatc cagcggttga acatcgagta
      901 ggagggctgg tggatgagga ggggggtgcc ggagtcgcgc aggataccgg ccgcctcgcg
      961 cgtcgtggcg gccgaatatg aggagatgcc ggggtagagc gcgaggcctt gctggacggc
     1021 gctcgtgagg gcgcccatgg tctcctcgag cggggtgtcc gggtcgtagc gatggctata
     1081 gaagatgtcg acccagtcga gccgcatgcg gtccaagctc tcttccaggc tcgacaggag
     1141 gtacttgcgc gacccccact ccccgtaggg gcccggccac atgtagtaac cggccttcgt
     1201 cgatatgacg agccggttgc gcaggccggc gaagtcggtg gccaggatct cgccgaagtt
     1261 cttctcggcg gcgcctgccg gcggaccgta gttgttggcg aggtcgaagt gcgtgatccc
     1321 gagttcgaag gcgcgttgca agacggcgct catcgaggcc agggggcggt cgtcgccgaa
     1381 gttgtgccag aggcccaggg agaccttagg aaggagcagg ccggagcggc cggagcgggc
     1441 gtacgtcatc gcgggggcgt cggtcacgcc cctaggttag cgcttcgtgc tcaggcgtct
     1501 gcgctcatgc gccgcagtgc gcgtggcttg cgggcctcgc cggtgcgcgg cgggtcgatc
     1561 aggtgcgata ggcgcacccc ggacagcact tcccggatgg cgagcgacgc gttgacccac
     1621 gccacgttca ggcggcagta gcccttgcgc tcctgcgtgg cgcagcgggt cttcgtctgg
     1681 cacgtgtcga cgatgagcgg ccccgacatc gtctcgatca cgtcgagcaa ggagatgctc
     1741 gtggggtcta ctatcaagtt cacgccgccg ttccgccctt ggcggttctc gacgtacccg
     1801 acctgggcga gccgtctcag caccttggcg aggaaggcag gggggatctt caggtcaccg
     1861 gcgatcctgg ccgcggccgc gcccgggttc tccgaggcgt agatgagcgc gtggatggcg
     1921 tagctctcct cgcggcgcag gagggtccgg tacggtgaga ggtcgatggg ctcagccacg
     1981 catccagggt agcaggtgaa tcggacccgc acctcctgga atgcactcta gccgcgcgtg
     2041 ccgtctggag c
//
LOCUS       DAOQYE010000376         2049 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_63421_length_2049_cov_3.197091, whole genome shotgun sequence.
ACCESSION   DAOQYE010000376 DAOQYE010000000
VERSION     DAOQYE010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63421_length_2049_cov_3.197091"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            104..1096
                     /locus_tag="PKN52_06775"
     CDS             104..1096
                     /locus_tag="PKN52_06775"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNQ99686.1"
                     /translation="MPVATLGLHHVTGIATRPQANIEFWVRLLGQRLVKRTVNYDDPQ
                     TYHFYFGGPLGQPGTIASFFPWQGAGPAVRGAGESVAVAYRVPPGSLAAWRERLDDHG
                     LAHTVRERLGEEVISFEDPDGIPLELVEAEPVGPRLNWPGAPLQDAIAPQGLHAVTVT
                     APRLDATVGVLLRALGMEVVAEDDGASGRRVRLAPGSPEAGDIPYPTGGYIDLVEARH
                     ESPGRLGAGGLHHVALRVADADIGEARETLISEGLKPTVVKDRTYFRSVYFKEPGGAI
                     FEVATDGPGFLVDEPPDELGLAFKLPPWLESERDFLRGRLPVTASPEYADRWRH"
     gene            1147..>2049
                     /locus_tag="PKN52_06780"
     CDS             1147..>2049
                     /locus_tag="PKN52_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99687.1"
                     /translation="MRKDGYTAMSGSVLPPEARLRELSAAAAAANHEFNYDEAMRHGR
                     EALAHVGLDPTADPGEWDACVAELDPATAASLANATTTLAISLFRRSDYVASLQVAQL
                     EAHLRRHIGDEIGEAFASIGLGWCHHAVGLYQHALAHHSAALDVLERLAPKSIAGPLN
                     GIARVYLDLGQPGLALEYAQRGLEAASASQHALRDTSTALRIVGLAHQQLNDPVAARS
                     ALEECFRRSDTYGKRFALLGHSMGAGIASLVAAACPGRIERLVAIEALGALPEAPENT
                     VSRLRDAVAAGRALPGKRLRVFADL"
BASE COUNT          306 a          748 c          699 g          296 t
ORIGIN      
        1 ccaggttcaa tagtcgggta gcgccagggc gccacgagga tctagagaac cctggtcaag
       61 agcggtaacg accgttgccg ccgctgctga taggctcttg ggcatgcccg tcgccacgct
      121 tggccttcac cacgtcaccg gcatcgccac gaggccccaa gccaacatcg agttctgggt
      181 gcgcctgctc ggccaacggc tcgtgaaacg caccgtcaac tacgacgacc cccagaccta
      241 ccacttctac ttcggcggcc cgctcggcca accgggcacc atcgcgagtt tcttcccgtg
      301 gcagggggcg ggcccggccg tgcgcggcgc cggcgaaagc gtcgcggtcg cctaccgcgt
      361 tccgcccggg agcctggcgg cttggcgaga acgcttggat gaccacggcc tggcccacac
      421 ggtgcgtgag cggttgggcg aggaggtcat ctccttcgag gaccccgacg gcatcccgct
      481 tgagctcgtc gaggcggagc ccgtcggccc gcgcctcaac tggcccggtg ccccgctgca
      541 ggacgccatc gcgccccagg ggctccacgc ggtgaccgtt accgcgcctc ggctcgacgc
      601 cacggtcggt gtcctactgc gcgcgctcgg catggaagtc gtagccgagg acgacggggc
      661 gagcggaagg cgcgtgcgcc tcgcccccgg ctcgccggag gcaggcgaca tcccctaccc
      721 caccggcggc tacatcgacc tggtggaggc tcgccacgag tcgcccggca ggctcggcgc
      781 cggcggcttg caccacgtcg cgctgcgagt ggccgacgcc gacatcggcg aggcgcgcga
      841 gaccctcatc agcgaagggc tgaagccgac ggtcgtcaag gaccgcacgt acttccgatc
      901 cgtgtacttc aaggagcctg gcggcgccat cttcgaggtc gccaccgacg gtcccggctt
      961 cctcgtcgac gagccgcctg acgaactcgg cctcgccttc aagctgccgc catggctcga
     1021 gagcgagcgc gacttcctca gggggcgcct gcccgtcacg gccagccccg agtacgccga
     1081 caggtggcga cattgacccc gacaggggcg ccgtctggtc aggcataatg agtacagctt
     1141 tgcgagatgc gtaaggacgg gtacacggcg atgtcaggaa gcgtgctccc acccgaggcc
     1201 cgcttgcgcg aactatcggc agcggcggcg gcggccaacc acgagttcaa ctacgacgag
     1261 gccatgcgcc acggtcgcga agcgctggcg catgtaggtc tcgacccgac cgccgacccc
     1321 ggcgagtggg acgcgtgcgt ggccgagctt gacccggcca cggccgccag cctcgcgaac
     1381 gcgacgacca cgctggcgat aagcctgttc cggcgcagcg actacgtggc gtcgctgcag
     1441 gtcgcgcagc tcgaagccca tctgcgccgc cacatcggcg acgagatcgg tgaggcgttc
     1501 gcctccatcg ggctcggttg gtgccaccac gccgtcgggc tctaccagca cgcgctcgcc
     1561 catcattccg cggctctcga cgtcctcgag cggttggcgc cgaagtcgat cgccgggccg
     1621 ttgaacggca tcgcgcgcgt ctacctcgac ttgggccagc cgggcctcgc gctggagtac
     1681 gcccagcgcg gtctggaggc ggcctcggcc tcccagcacg cgctgcgcga cacctcgacc
     1741 gccctcagga tcgtcggact ggcccatcag caactgaacg accccgtggc tgcaaggagc
     1801 gcgctcgagg agtgcttccg gcgttcggac acgtacggta agcgcttcgc cctgctcggt
     1861 cattcgatgg gcgcgggcat cgccagcctg gtcgccgcgg cctgccccgg gcggatcgag
     1921 cgtctggtcg cgatcgaggc cctgggcgcg ctgccggagg cgccggagaa cacggtatcg
     1981 cgtctgcgcg acgcggtcgc ggccggccgc gccctgccgg gcaagcggct gcgcgtgttt
     2041 gccgacctc
//
LOCUS       DAOQYE010000377         2047 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_63538_length_2047_cov_3.133032, whole genome shotgun sequence.
ACCESSION   DAOQYE010000377 DAOQYE010000000
VERSION     DAOQYE010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63538_length_2047_cov_3.133032"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            602..1033
                     /locus_tag="PKN52_06785"
     CDS             602..1033
                     /locus_tag="PKN52_06785"
                     /inference="COORDINATES: protein motif:HMM:NF025178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HNQ99688.1"
                     /translation="MSDLSDPNVPGTRLTSRHVVATVDEIPIGGRKIVTVGNRSIGVF
                     NIGGRYFALRNTCPHQQAPLCEGRVMGTTLPSRPGEYHLGLQGQILRCPWHAWEFDLT
                     TGRSLFDPTKCRVKTFDVAVETVQVETFRTEVVGQVVVIYA"
     gene            1233..>2047
                     /locus_tag="PKN52_06790"
     CDS             1233..>2047
                     /locus_tag="PKN52_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNQ99689.1"
                     /translation="MPNDNATNTAGGRPSVTPEQFAKDFAAMRSAISSVVVGQERVVE
                     ELLVAALARGHVLLEGVPGLGKTRLVHTFADVSSLNFSRIQFTPDLMPADVTGTNVFI
                     EAAAQSRFEFQPGPVFTNVLLADEINRATPKTQSALLEAMQERAVTVAGKRYALDEPF
                     MVLATQNPLEMEGTYPLPEAQLDRFLFKVIIPRPDAATLQRILIVTTGTTEAQAGAVV
                     DKERLLILQAMLRAVPIAEAAISYVVRLVEATHAHPLVRLGASPRGGSHPTGRV"
BASE COUNT          352 a          661 c          664 g          370 t
ORIGIN      
        1 aagccggcca cgacacggcg ctgccgggac gcatcttccg ccagggcatg ggtagccgct
       61 cccagaacgc gtcatcgatg ccttgatgga catctgcgtc tacccgggtg acctgtgtgc
      121 cggcctccgg ctgcgagtca ggggctcctt gggagccatc ggctggagct ttcgtattcg
      181 atgggtcctt gacggcgctt gacacttcga gcctcctgcg gagatcaact catgagcgag
      241 cgaagacgac tgccgcgtca ggctcgttga caatcctatc acaatgttct agtatgctac
      301 cacaaattac gctcggaggt cgccggctgg tggtgcgctt tattggttat agctttgcga
      361 acgcactgtt catgaagcac gcgtatgcct cctcggatcg gctgaacaac ttgccttgtt
      421 gcctcctttg ccttggagtt ctccgccggc tcgcggccga gacgactgac gggaacgggt
      481 gccgcgatcg tgaccgggcg gaccgaagac ctatcaggcg gagactgctg gccccgcgag
      541 tcgctcatac cgaaggaccg gctgaagagg cgttgcggtt ctgcggtccg gtgcctgcgc
      601 catgagcgat ctgtcggacc cgaacgtgcc ggggacgcgc ctgacgtctc gacatgtcgt
      661 cgctacagtc gacgagatac ctatcggtgg caggaagatc gtaactgtgg gcaatcgctc
      721 catcggggtg ttcaacattg gtgggcgata cttcgccctg cgcaacacat gcccccacca
      781 gcaagcaccg ctatgcgaag gaagggtgat gggtacgacg cttccttcgc gcccaggcga
      841 ataccacctt gggcttcagg ggcagatact gagatgtccc tggcatgcgt gggagttcga
      901 tctgactacg ggtaggtctt tattcgaccc gaccaagtgc agggtgaaga cctttgatgt
      961 cgctgtcgag accgtccagg tagagacgtt ccggaccgag gtcgtaggtc aggtggtcgt
     1021 gatatacgcc tgagggagtc gggcatgcac ggggatgtgg cgcatgctcc cggtccactt
     1081 gtagttgccg tggctagagt catgggggcc agactggcct aggagcaccg gggatcaacc
     1141 actaagcggc ctcgggtgtt gttgctacgc ggctatactc cccgtactcc caagcgccgc
     1201 cacggcgcac ccgccaaggc cagaggtgca aggtgccgaa cgacaacgcg accaacacag
     1261 ccggggggcg tccctcagta acgcccgaac agttcgccaa ggacttcgcg gccatgcgct
     1321 cggccatctc gagcgtcgtg gtcggtcagg agcgggtcgt cgaggagctg ctcgtcgcgg
     1381 cgctcgcccg cggtcatgtg ctcctcgagg gcgtgccggg cctcggcaag acgcggctcg
     1441 tgcacacgtt cgccgacgtc tcgtcgctga acttctcgcg catccagttc acgcctgacc
     1501 tcatgccggc cgatgtcacg ggcacgaacg tcttcatcga ggccgcggcg cagagccgct
     1561 tcgagttcca gcccgggccg gtgttcacga acgtgctcct ggccgacgag atcaaccggg
     1621 ccacgcccaa gacgcagtcg gcgctgctcg aggccatgca ggagcgggcc gtaaccgtgg
     1681 cgggcaagcg ctatgcgctc gacgagccgt tcatggtcct cgcgacccag aacccgctgg
     1741 agatggaggg tacctacccg ctgcccgagg cgcagctcga ccggttcctc ttcaaggtca
     1801 tcatcccgcg gccggacgcc gccacgctgc agcgcatcct gatcgtcacg accggcacca
     1861 cggaggcgca ggcgggcgcg gtggtggaca aggagcgcct cctcatcctg caggccatgc
     1921 tccgcgccgt gccgatcgcc gaagccgcca tctcgtacgt cgtgcgcctc gtcgaggcca
     1981 cccacgcgca cccactcgtc cggttgggtg cgagcccccg cgggggctcg cacccaaccg
     2041 gacgagt
//
LOCUS       DAOQYE010000378         2035 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_64129_length_2035_cov_2.788384, whole genome shotgun sequence.
ACCESSION   DAOQYE010000378 DAOQYE010000000
VERSION     DAOQYE010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64129_length_2035_cov_2.788384"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="PKN52_06795"
     CDS             complement(<1..383)
                     /locus_tag="PKN52_06795"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HNQ99690.1"
                     /translation="MKKLIITLLAAITASGAFAQYSFPDIPANHWAGDAVDRISSLGI
                     VIGFPDGTFRGNEAFTRYQAALVVSRLLDVINDNLNSALALTQADVDSLRNAVQELAS
                     DVAAQGVRLSAAESAIAGLSDDVTA"
     gene            793..1674
                     /gene="thyX"
                     /locus_tag="PKN52_06800"
     CDS             793..1674
                     /gene="thyX"
                     /locus_tag="PKN52_06800"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HNQ99691.1"
                     /translation="MARIIVPEAEALIDQELGVLDHGVVRLVDYLGDDARIVQAARVS
                     YGDGTKTVREDGALIDYLLRNRHTSPFEQVILTFHVKLPIFVARQWIRHRTARLNEIS
                     GRYSVMRDEFYVPNAREVRFQDKRNRQGGDGREVPPELRERVISVLRSGQERTYVEYE
                     GLLEDDIARELARVNLPLSLYTEMYWQIDLNNLFHFLRLRLDWHAQYEIRAYGDVMAR
                     IAQAVAPMAYAAFEEHELHGRSFSRTELDVLRAALDPERLEAALAASGLRATRRDDLM
                     RKLALMRKPAAPGEAPA"
     gene            1685..>2035
                     /locus_tag="PKN52_06805"
     CDS             1685..>2035
                     /locus_tag="PKN52_06805"
                     /inference="COORDINATES: protein motif:HMM:NF014629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HNQ99692.1"
                     /translation="MRYRVVAVGKLKSAFYREACEHYLKRLGPLATCEVIEVREAREA
                     DVEATKLAEGAALLAKVQGLSVALDEHGRTLDTRALARRVSALENRGVSQATILVGGA
                     EGHGRDLLGAVDETW"
BASE COUNT          328 a          680 c          704 g          323 t
ORIGIN      
        1 ttggcggtca cgtcgtcgga gaggccggcg atggcgctct cggcggcgga cagcctcacg
       61 ccctgggcag cgacgtcaga ggcgagctct tgcacggcgt tgcgcagcga gtcgacgtca
      121 gcctgggtga gcgctagcgc cgagttgagg ttgtcattga tgacgtcaag caggcggctg
      181 acaacgagag cggcttggta acgcgtgaag gcttcgttac cgcggaaggt tccgtcgggg
      241 aagccgatga cgatgccgag gctggagatg cggtcgacgg cgtcgccggc ccagtggttc
      301 gccgggatgt ccgggaacga gtactgagcg aaggcgccgc tggccgtgat cgccgccagg
      361 agcgtgatga ttagcttctt catgaattcc taccccttga actggtgaag tcttcctgct
      421 cagtgcgctt cacgagccgc cgggcgcagc caccgtgagt gtccgagttt cctcgccggt
      481 cttgcgccac tcgatctcgc gtcgctcact gggttcccga gcttccctgg ccggttcaaa
      541 aggtagcacc tccttccagt gcggccacgg acgacacggg tccaccggga ttagaccacg
      601 cgcgctgcgc gcgtgtcaag gcgcatgtgg catcgatgag aagcctttgg ggccatgcgc
      661 atgggtaaaa cccccatagg tgaagcacgt cactcacggt cgcctcatga gaggacggcg
      721 cgtcgaacgg tcgggcggga ggcgtcccga caccccgagg ccggtcgccc accacctggt
      781 atcgtcgcgc ccatggctcg catcatcgtg cctgaagcgg aagcgctgat cgaccaggag
      841 ttgggggtct tggaccacgg ggtcgtgcgc ctcgtcgact accttggcga cgacgcgcgc
      901 atcgtccagg ccgcccgcgt ctcgtacggg gacggcacga agaccgttcg cgaggacggc
      961 gccctcatcg actaccttct ccggaaccgg cacacgagcc cgttcgagca ggtcatcctc
     1021 accttccacg tcaagttgcc gatcttcgtg gccaggcagt ggatccgtca ccgcacggcg
     1081 cgactgaacg agatctccgg gcggtactcc gtcatgcgcg acgagttcta cgtcccgaac
     1141 gcccgcgagg tgcggttcca ggacaagcgc aaccgccaag gcggcgatgg gcgcgaggta
     1201 ccgccggagc tgcgcgagcg cgtgatctcc gtgctgcgct ccggccagga acgcacctac
     1261 gtcgagtacg aagggttgct ggaagacgac atcgcccgcg aactggccag ggtgaacctg
     1321 ccgctgagcc tctacaccga gatgtactgg cagatcgacc tcaacaacct gttccacttc
     1381 ctgaggttgc gcctggactg gcacgcccag tacgagatca gggcgtacgg cgatgtcatg
     1441 gcgcggatcg cgcaggcggt cgctcccatg gcgtacgccg ccttcgagga gcacgagctc
     1501 cacggccgct ccttctcgcg caccgagctc gacgtcctgc gcgcggcgct cgaccccgag
     1561 cggctcgagg ccgccctggc cgccagcggc ctgcgcgcca cgcgccggga cgatctcatg
     1621 cgcaagctcg cgctcatgcg caagcccgcg gcgcccggcg aggcgcccgc ctgaggcgac
     1681 gcgggtgcgt taccgcgtcg tcgcggtcgg gaagctgaag tccgcgttct accgcgaggc
     1741 ttgcgagcac tacctcaaac gcctcggtcc cctggcgacg tgcgaggtca tcgaggtgcg
     1801 cgaggcgcgc gaggctgacg tcgaggcgac caagctcgcc gagggcgccg ccctcctcgc
     1861 gaaggtccag gggctctccg tcgccttgga cgagcatggc cggacgctcg acacccgggc
     1921 cttggcccgc cgtgtgagcg cgctggagaa tcgcggcgtc agccaggcga ccatcctggt
     1981 cggcggggcg gaagggcacg ggcgcgacct gctcggggcg gtcgacgaga cctgg
//
LOCUS       DAOQYE010000379         2032 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_64269_length_2032_cov_3.421851, whole genome shotgun sequence.
ACCESSION   DAOQYE010000379 DAOQYE010000000
VERSION     DAOQYE010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64269_length_2032_cov_3.421851"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="PKN52_06810"
     CDS             complement(<1..322)
                     /locus_tag="PKN52_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99693.1"
                     /translation="MRRTLERARARLALAALLRSATWAIVAAAVLGAALVAGVRLGAL
                     PFPADGRLFGLPAAVWAIGGALVAGICVVVAAWRRTPGIARTARVVESHFGLSERLST
                     SLELG"
     gene            867..1784
                     /locus_tag="PKN52_06815"
     CDS             867..1784
                     /locus_tag="PKN52_06815"
                     /inference="COORDINATES: protein motif:HMM:NF013341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIO1 family regulatory kinase/ATPase"
                     /protein_id="HNQ99694.1"
                     /translation="MSSKLDDRTDGLESFGFRSGMSDSEYAAGRRKRDRRKPSAKRRA
                     ADLVLTEADRETADTETEFADADMQRLYARGYFDRLIGQVKGGKEATVYLVGLEDELF
                     AAKVYADMDARSFRDDSAYWAGITITDVRIAKAMKHRTRAGQAARAAFWVGREYMYLW
                     HLHRAGLPVPRPAVGPEATAISSAGAVVLMEFIGEGDVPAPRLSDVRLTAAEAQSAYA
                     QSVSILVRLTELGLVHGDFSTYNLLWHGGKVVLIDVPQMLELRNGRTAHELLARDVTS
                     LVTSFRRLRVNADEAELRRTVLAAVAKDS"
     gene            complement(1816..>2032)
                     /locus_tag="PKN52_06820"
     CDS             complement(1816..>2032)
                     /locus_tag="PKN52_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99695.1"
                     /translation="TVHVLQLVGWMPVHTLPLRFPTWAGVWLGTFPTVEGLLAQVMAV
                     ALVVGSYFLAERSKVRTRQVARSARAA"
BASE COUNT          349 a          664 c          743 g          276 t
ORIGIN      
        1 cccccagctc gaggctcgtg ctgagccgct ccgagaggcc gaagtggctc tccacgacgc
       61 gcgccgtccg ggcgatgccg ggcgtgcggc gccatgcggc cacgaccacg catatgccgg
      121 cgacgagggc gccgccgatc gcccaaacgg cagccggcag accgaacaag cgcccgtccg
      181 cagggaacgg cagggcgccg agccgaacgc cggcgaccag cgccgcgccc agcaccgccg
      241 cggccacgat cgcccacgtg gcggagcgga gcagcgccgc gagcgccagg cgtgcccgcg
      301 cgcgctctag ggtgcggcgc atcagtcctg gaccggctgg aacgcgaaga cctcgccggc
      361 gacgccaagt gcgcgaaagg tctggtgcgg cggggcgcga cgaggcatgg cttatactaa
      421 gggctttgca aacctgtttt caaagatgcg gtgcggagcg ggacaccggt tgcgaggcgc
      481 gctcgtgccg acgcgctgga agtaaggcgc ttgacagcgg cctccgcccg gcacatactc
      541 gccacgtagc ccgaaggccg ttctctcgga tctggcacga cgacgtgtgc ggtgggaacg
      601 gcaagtggcg acggaaggcg gtgttcgatg gctcgtgacg atgtctttgt gaccgtaagt
      661 cggcggcagg gccgcgagca acgcttgccg ggcgtgcagc tttaaggcga tgggctgaac
      721 cttccgaaca agcaagctat agcgaagcgc caggtgggcg ccatgtcgga acgtctccgg
      781 tagggcggct cggactggag cgttcgcggc caccacgagc aggatcgccc cgtggcgcca
      841 tggtggcgcg actccggagg aagagcttga gtagcaagct agacgacagg accgacggcc
      901 tggagtcgtt cggtttcaga agcgggatga gcgatagcga gtacgcagcg ggtcggcgca
      961 agcgtgaccg caggaagccg agcgccaagc gtagggccgc ggacctggtg ttgacggagg
     1021 cggaccggga gacggcggat accgagacgg agttcgcgga cgccgacatg cagcggctct
     1081 acgcgcgcgg ttacttcgac aggctgatcg gccaggtcaa gggcgggaag gaggcgaccg
     1141 tctacctggt gggcctcgag gacgagctct tcgcggccaa ggtatacgcc gacatggacg
     1201 cgcgctcgtt ccgcgacgac tccgcgtact gggcaggcat caccatcacg gacgtgcgca
     1261 tcgccaaggc gatgaagcac cggacgaggg ccggccaggc ggctcgggcc gccttctggg
     1321 tcggcaggga gtacatgtac ctctggcacc tccacagggc cgggctgccc gtgccgcggc
     1381 cggccgttgg gccggaggcg acggccatca gctcggcagg cgcggtcgtg ctcatggagt
     1441 tcatcgggga gggtgacgtc ccggcgccac gcctctccga cgtccgcctg accgcggccg
     1501 aggcacaatc ggcttacgcc cagagcgtct cgatactcgt ccgtctcacg gagctcggtc
     1561 tcgtgcatgg agacttctcg acgtacaacc tcctctggca cggcggcaag gtggtcctga
     1621 tcgacgtgcc gcagatgctg gagctccgca acggccgcac ggcccatgaa ctgttggcgc
     1681 gcgacgtcac ctccctcgtc acgtcgttcc gcaggttgcg cgttaacgcc gacgaggccg
     1741 agctgcgacg gaccgtgttg gcggcggtcg ccaaggactc gtgatagtga gacgtggctg
     1801 gcgacggttc gccggctagg ccgccctcgc cgagcgcgcc acctggcgcg tgcgcacctt
     1861 ggagcgctcg gcgaggaagt agctgcccac cacgagcgcg acggccatga cctgagcgag
     1921 caggccctcg accgtaggga acgtccctag ccacacgccg gcccacgtcg ggaaccgtag
     1981 cggcagcgta tggacgggca tccacccgac gagctgcagg acgtgcaccg tg
//
LOCUS       DAOQYE010000380         2032 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_64302_length_2032_cov_1.813859, whole genome shotgun sequence.
ACCESSION   DAOQYE010000380 DAOQYE010000000
VERSION     DAOQYE010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64302_length_2032_cov_1.813859"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2032
                     /gene="nifJ"
                     /locus_tag="PKN52_06825"
     CDS             <1..>2032
                     /gene="nifJ"
                     /locus_tag="PKN52_06825"
                     /inference="COORDINATES: protein motif:HMM:TIGR02176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HNQ99696.1"
                     /translation="PFSVEHFIAALPASVKKIAVLDRTKEPGSIGEPLYEDVRTAIGE
                     AMEAGKAQFKGWPTVIGGRYGLGSAEFTPAMVKAVFDNLGAAKPKAEFTVGIHDDVTG
                     MSLPVDESFQLDAQGVVECLFVGLGADGTVGANKNSIKIIGDETDNSAQGYFVYDSKK
                     SGTYTISHLRFGPRQIQKPYLISKADFLACHKFSFLEKLDVLAWAKDGGTFLLNSPFP
                     ADKVWDELPIEVQKRIIDKKLKFYVIDGMAIAEKAGMGGRINTVMQTAFFKISGVLPE
                     AEAVKLIKKYTEKTYARKGADVVANNLAAIDLALSATQEVKIGQANSKHRMKGTVTAD
                     APAFVKEVLGEIAANRGDKLKVSQMPDDGTFPTATTKYEKRNIAEKIPAWDPELCIQC
                     GQCSVVCPHAVIRLKAYKPEALNGAPATFKSAAAKGKEFEGMKATLQVAPEDCTGCGV
                     CINQCPVKDKAVEGRKAINFVDQIAVREEESKNWDFFLSLPETPGLNLSTIKGISMKR
                     PLFEFSGACAGCGETPYIKMMSQLFGDRAVIANATGCTSIYGGNLPTTPYCTREDGRG
                     PAWSNSLFEDAAEFGMGMRLNADKMGQYARELVGANKAAIGALADKLLSNAQADDEAV
                     EAQRANVAELKKLLGGKADQWAKELVNVADHLVKRSVWIFGGDGWAYDIGFGG"
BASE COUNT          401 a          733 c          613 g          285 t
ORIGIN      
        1 cccttcagcg tggagcactt catcgccgcc ctgccggcga gcgtcaagaa gatcgccgtc
       61 ctcgaccgca ccaaggagcc cggctccatc ggcgagcccc tgtacgagga cgttcgcacc
      121 gccatcggcg aggccatgga ggccggcaag gcccagttca agggctggcc gacggtcata
      181 ggcggccgct acggcctggg ctcagctgag ttcaccccgg cgatggtcaa ggccgtcttc
      241 gacaacctgg gcgccgccaa gcccaaggcc gagttcaccg taggcatcca cgacgacgtg
      301 accggcatga gcctgcccgt cgacgagtcc ttccagctgg acgcccaggg cgtggtcgag
      361 tgcctcttcg tgggcctcgg cgccgacggc accgttggcg ccaacaagaa ctccatcaag
      421 atcatcggcg acgagaccga caacagcgcc cagggctact tcgtgtacga ctccaagaag
      481 tcgggcacct acaccatcag ccacctgcgc ttcgggcccc gccagatcca gaagccctac
      541 ctgatcagca aggccgactt cctggcctgc cacaagttca gcttcctcga gaagctggac
      601 gtcctggcct gggccaagga cggcggcacc ttcctcctca acagcccctt ccccgccgac
      661 aaggtctggg acgagctgcc gatcgaggtg cagaagcgca tcatcgacaa gaagctcaag
      721 ttctacgtga tcgacggcat ggccatcgcc gagaaggccg gcatgggcgg ccgcattaac
      781 accgtcatgc agaccgcgtt cttcaagatc tccggcgtac tgcccgaggc cgaggcggtc
      841 aagctcatca agaagtacac cgagaagacc tacgccagga agggcgccga cgtcgtggcc
      901 aacaacctgg cggccatcga cctcgcgctc tccgccaccc aggaagtcaa gatcggccag
      961 gctaactcca agcaccgcat gaagggcacc gtgaccgccg acgcccccgc cttcgtcaag
     1021 gaggtcctgg gcgagatcgc ggcgaaccgc ggcgacaagc tcaaggtgag ccagatgccc
     1081 gacgacggca ccttccccac cgccaccacc aagtacgaga agcgcaacat cgccgagaag
     1141 atccccgctt gggatcccga gctctgcatc cagtgcggcc agtgctccgt ggtctgcccg
     1201 cacgccgtca tccgcctcaa ggcctacaag cccgaggcct tgaacggcgc gccggcgacc
     1261 ttcaagagcg ccgccgccaa gggcaaggag ttcgagggca tgaaggccac gctccaggtg
     1321 gcccccgagg actgcaccgg ctgcggcgtc tgtatcaacc agtgcccggt caaggacaag
     1381 gccgtagagg gccgcaaggc catcaacttc gtggaccaga tcgccgtccg cgaggaggag
     1441 tccaagaact gggacttctt cctctcgctc cccgagaccc ctggcctcaa cctgtccacc
     1501 atcaagggca tctcgatgaa gcgcccgctc ttcgagttct ccggcgcctg cgccggctgc
     1561 ggcgagaccc cgtacatcaa gatgatgagc cagctcttcg gcgaccgcgc ggtcatcgcc
     1621 aacgccaccg gctgtacctc catctacggc ggtaacctgc ccaccacgcc ctactgcacc
     1681 cgcgaggacg gccgcggccc ggcctggtcc aacagcctct tcgaggacgc cgccgagttc
     1741 ggcatgggca tgcgcctgaa cgccgataag atgggccagt acgcccgcga gctcgtcggc
     1801 gccaacaagg ccgccatcgg cgccctggcc gacaagctcc tctccaacgc ccaggccgac
     1861 gacgaagccg tcgaggccca gagggcgaac gtggccgagc tcaagaagct cctgggaggc
     1921 aaggccgacc agtgggctaa ggagctggtc aacgtggccg atcacctggt caagcgctcc
     1981 gtctggatct tcggcggcga cggctgggcc tatgacatcg gcttcggcgg cc
//
LOCUS       DAOQYE010000381         2031 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_64333_length_2031_cov_3.205466, whole genome shotgun sequence.
ACCESSION   DAOQYE010000381 DAOQYE010000000
VERSION     DAOQYE010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64333_length_2031_cov_3.205466"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..793
                     /locus_tag="PKN52_06830"
     CDS             <1..793
                     /locus_tag="PKN52_06830"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ99697.1"
                     /translation="KAALVGFVIFTLFLICAVFAPALAPANPNRANFSMVLHPPGAAA
                     LLGTDELGRDILSRILFGARASFLVAASTTAIAAVLGVLLGLLAGYYGGLVDAIIMRA
                     ADLLLAFPSFLVALVIVGILGPGLMNVILAVGVLFVPRFVRLMRASVLSVRENEYIEA
                     MAALGSRTPRILFQHVMPNAMAPIIALTAIVSGEAILIGSGLGFLGLGAQPPLAEWGA
                     MLSEGRVYLTTAPHVAAFPGLAIMGLVVALNLIGDGVRDALDVRL"
     gene            complement(878..1270)
                     /locus_tag="PKN52_06835"
     CDS             complement(878..1270)
                     /locus_tag="PKN52_06835"
                     /inference="COORDINATES: protein motif:HMM:NF021221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HNQ99698.1"
                     /translation="MTLPSAPGPTTDPSLTAVRDWAMIAHLSALVGLLGNGIGFVLGP
                     LVVWLWKRDDHEFIREQTIEALNFQITMMLAVVAGVILVFTVIMLVPGFLVIIAAGLM
                     MVIYPIVAATQAKRGMHYRYPLSLRLVK"
     gene            complement(1326..1613)
                     /locus_tag="PKN52_06840"
     CDS             complement(1326..1613)
                     /locus_tag="PKN52_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99699.1"
                     /translation="MLRRFTAYVIALLELAEAEATSAVRAGLVRAGRAVALLVAGIGL
                     LVAAAALLVWAAYQALLPYWGKAGSGAACAVLLALFGAIFLWLAASRDRKR"
     gene            complement(1621..>2031)
                     /locus_tag="PKN52_06845"
     CDS             complement(1621..>2031)
                     /locus_tag="PKN52_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99700.1"
                     /translation="DPRVTLTTELSDPGAIFQVRRKALDLPHRDEKEGYRMATTNKDI
                     EALRKDVEALKKEFKSMASDSGNLLETARSELEAKAEELMESIKSAGSSAIEQGEEVL
                     HKAEEKIAEKPLTSVLVTLGIGVALGWLLRRKMS"
BASE COUNT          322 a          674 c          648 g          387 t
ORIGIN      
        1 caaggcggcg ctggtcggct tcgtcatctt caccctcttc ctgatctgcg ccgtgttcgc
       61 tccggccctg gcaccggcca acccgaacag ggccaacttc tccatggtcc ttcacccgcc
      121 tggcgccgcc gccctcctcg gcaccgacga gctcgggcgt gacatcctca gccgcatcct
      181 tttcggggcg agggcttcct tcctcgtcgc cgcgtcgacc accgccatcg ccgcggtgct
      241 aggggtgtta ctcgggctac tggccggcta ctacggaggt ctggtcgacg cgatcatcat
      301 gcgggccgcc gacctgctgc tcgccttccc gagcttcctc gtcgcgctgg tcatcgtcgg
      361 tatcctcggt cctggcctaa tgaacgtgat cctggccgtc ggcgtcctct tcgtgccgcg
      421 cttcgtgcgg ttgatgcggg cctccgtgct gtccgtgcgg gagaacgagt acatcgaggc
      481 catggcggcc ctcggcagcc gcacgccccg catcctcttc cagcacgtca tgcccaacgc
      541 catggcgccg atcatcgcgt tgacggcgat agtcagcggg gaagccatcc tcataggttc
      601 aggactgggt ttcctcggcc tcggggctca gccgcccttg gccgaatggg gtgcgatgtt
      661 gagcgaaggc cgcgtctact tgacgaccgc gccgcacgtc gccgcctttc ccggtttagc
      721 cattatgggt ctggtggtag cactcaacct cattggtgat ggtgtgagag atgcattgga
      781 cgtgcggctt taagaaggta agagcctcat agccggcagg atggtcggag ctacctcacc
      841 ggttggatga aatgccggta ccgtcaccgg cttacgctta cttgaccaag cggagcgaca
      901 acgggtaacg gtagtgcata ccgcgtttgg cctgggtcgc ggcgacgatg gggtagatga
      961 ccatcatgag ccccgccgcg atgatcacca agaacccggg gacgagcatg atgaccgtga
     1021 agacgagtat cacgcctgca acgaccgcga gcatcatcgt gatctggaag ttgagggcct
     1081 cgatggtctg ctcgcggatg aactcgtggt cgtcgcgttt ccagagccat acgaccagcg
     1141 gcccaagcac gaacccgatg ccgttcccca acaggccgac cagggccgag aggtgggcga
     1201 tcatggccca atcgcgtacc gccgtgagcg agggatcggt ggtggggccg ggggcgctcg
     1261 gaagggtcat cttgtcgcca ttacaccacc ggccggactg accggtcgta gcgggctccg
     1321 ggccgtcagc gtttgcggtc gcgcgaagcg gccagccaga ggaagatggc gccgaagagc
     1381 gcgagcagta ccgcgcatgc ggccccggaa ccggccttgc cccagtaagg tagcagcgcc
     1441 tggtaggcgg cccacacgag cagcgccgcg gcggcgacca gtaggccgat gccggcgacc
     1501 aacagcgcca cggccctgcc ggcgcgcacg aggccagcgc gtaccgcgct ggtcgcctcg
     1561 gcctctgcca actccagcag ggcgatgacg taagcggtga aacgccggag cacggcgcgc
     1621 tcagctcatc ttgcgccgca acagccagcc gagcgccacg cctatgccca gcgtgaccag
     1681 caccgaggtg agcggtttct cggcgatctt ctcctcggcc ttgtgcagga cttcctcgcc
     1741 ctgttcgatg gcggagctgc ccgcgctctt gatgctctcc atgagctctt cggccttggc
     1801 ttccaactcg ctgcgagcgg tctccaacag gttgccgcta tcgctggcca tgctcttgaa
     1861 ctccttcttc agtgcttcaa cgtctttacg gagtgcttcg atgtctttgt tcgtggtagc
     1921 catgcggtag ccctccttct catcacggtg cggcaggtcc agcgccttgc gccgcacttg
     1981 gaagatagca ccggggtctg agagttccgt tgtgagcgtc acgcgcgggt c
//
LOCUS       DAOQYE010000382         2031 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_64335_length_2031_cov_2.886640, whole genome shotgun sequence.
ACCESSION   DAOQYE010000382 DAOQYE010000000
VERSION     DAOQYE010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64335_length_2031_cov_2.886640"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(78..539)
                     /locus_tag="PKN52_06850"
     CDS             complement(78..539)
                     /locus_tag="PKN52_06850"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HNQ99701.1"
                     /translation="MGARERSGADSGGPIALDTSVIIAALLENHEHHERAFDIVRTAL
                     ATGTILPLPALLEAYAVMTRLPPPLRLPPQATMWLLRTTFEATAELVAAARTDAWPLI
                     DGLASSGISGGATFDAHIVLCARLGGASKLATFNARHFRRLDLGGIELLEP"
     gene            complement(529..786)
                     /locus_tag="PKN52_06855"
     CDS             complement(529..786)
                     /locus_tag="PKN52_06855"
                     /inference="COORDINATES: protein motif:HMM:TIGR01439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HNQ99702.1"
                     /translation="MDSAGRLVIPKGLREAAGLRAGVPLEVVARDGRIEIEPAPAAVE
                     LFLKAGIGVARIGAGPEGAEPELTAADVEAVRDDLRAERGR"
     gene            1214..>2031
                     /locus_tag="PKN52_06860"
     CDS             1214..>2031
                     /locus_tag="PKN52_06860"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HNQ99703.1"
                     /translation="MQVKTTNLLRAAAVSLMALLVAFASAQSRTDARVLEQETQIGIL
                     DLYEALTPLKSVASFMTVGAHPDDERSSQIALLSKGLGVRSITVTANRGEGGQNSIGT
                     EYLQALGVLRSREMEQASAAFNVELFFLSESFDDPIFDFRFSKSAVETLEIWGEDVMM
                     EKLVRAIRQSRPDVIFTNFQNVFGQHGNHRAMAYAAEQAFTLAADPNAYPEHFEMGLE
                     PWQAAKFYLPSGRGSGQADFELETTLSIPTGGYDQVYGATYEQLGQQSRAYHRS"
BASE COUNT          350 a          723 c          637 g          321 t
ORIGIN      
        1 tcggacgacg atctggaggc gctcgtgctg aaggactggg cgcggttcgg gctgatccgt
       61 tagcggcgcg gcgccggtca gggttcgagc agttcgatgc cgccaaggtc gaggcgccgg
      121 aagtggcgcg cgttgaaggt ggccagtttg ctcgcaccac cgagacgcgc gcataggacg
      181 atatgggcat cgaaagtggc gccgccggag ataccgctcg aggccaggcc gtcgatgagc
      241 ggccatgcgt ccgtcctggc cgcggccacg agttcggccg tggcctcgaa cgtcgttcga
      301 agcagccaca tggtggcctg cggtggcaag cgtagcggag gtggcagtct ggtcatgacc
      361 gcgtacgcct ctagcaatgc gggcaacggc aggatggtcc cggtcgcgag cgccgtgcgc
      421 acgatgtcga aggcgcgctc atggtgttcg tggttctcca ggagcgcggc gatgatgacg
      481 ctcgtatcca gcgcgatcgg accgcccgag tctgccccgc ttcgctccct agcgcccacg
      541 ctcggctcgc aggtcatccc ttactgcttc cacgtctgcg gccgtcaact ccggttccgc
      601 gccctccggc ccggcgccga tgcgcgccac gccgatgcct gctttaagga acagctccac
      661 cgcggcgggc gcgggctcga tctcgatgcg accgtcgcgc gctaccacct ccaacggaac
      721 gccggcccgt aggccagctg cctcgcgcag ccccttaggg atgaccagcc ttccggcaga
      781 atccatggta acaatcatgg tagcagttta ccatgcagtt cgcttcggca tggcgcgcac
      841 gccacaccgg aagcggcaag cgcgttctcg tgtctgccgc tcgccagggg agccttagcg
      901 gcgctcatga agcgcgtgtg taccacttcg ccgcaccgct cggttgacat ccccgcggca
      961 cccccgtagg ctttcgtagg gacatttaga aaccccattg ctaaaggtgt ttcatagtcc
     1021 gagaacgcgt cgtcggcgaa atcgcgcccg accgccttcg accacacttc agggccgcgg
     1081 aggtgaaggt tcccaagagc gcaggacagg ccgcacgcca ctccaggtca ggtcggctag
     1141 cgccggcagc cgctggacag ggaacgagac cgggccctgg accaacctcg aacacccacg
     1201 gaggcacaac ctgatgcaag tgaagaccac gaaccttctt cgcgcggcgg cagtctcgct
     1261 catggcgctc ctcgtcgcgt tcgcgtcggc ccagagccgc acggacgccc gagtgctcga
     1321 gcaagagacg cagatcggca tcctcgacct ctacgaggcg ctcacgcccc tcaagtccgt
     1381 cgccagcttc atgaccgtcg gcgcccaccc tgacgacgag cgctccagcc agatcgcgct
     1441 cctctcgaag ggcctcggcg tgcgcagcat caccgtcacg gccaaccgcg gcgaaggtgg
     1501 tcagaactcg atcggcaccg agtacctgca ggccctcggc gtgttgcgca gccgggagat
     1561 ggagcaggcc agcgcggcct tcaacgtcga gctgttcttc ctcagcgaga gcttcgacga
     1621 ccccatcttc gacttccgct tctccaagtc cgctgtcgag accctcgaga tctggggcga
     1681 ggacgtcatg atggagaagc tcgtccgcgc catccgccag agccgcccgg acgtcatctt
     1741 caccaacttc cagaacgtct tcggccagca cggtaaccac cgcgccatgg cctacgcggc
     1801 cgagcaggcg ttcaccctcg ccgccgaccc gaacgcctac cccgagcact tcgagatggg
     1861 cctcgagcct tggcaggccg ccaagttcta cctgcccagc ggccgcggct ccggccaggc
     1921 cgacttcgag ctggagacga ccctctccat cccgaccggc ggttacgacc aggtctacgg
     1981 cgccacgtac gagcagctcg gccagcagtc gcgcgcctac caccgcagcc a
//
LOCUS       DAOQYE010000383         2026 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_64601_length_2026_cov_2.658549, whole genome shotgun sequence.
ACCESSION   DAOQYE010000383 DAOQYE010000000
VERSION     DAOQYE010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64601_length_2026_cov_2.658549"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(69..1319)
                     /locus_tag="PKN52_06865"
     CDS             complement(69..1319)
                     /locus_tag="PKN52_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99704.1"
                     /translation="MLIDGVLHRRCHDKGEVGYHSLCGSFPVGRATYRPVGERNAKTV
                     VPLELEAGLVDHATPALAYSIALDWSEVNSRRYVKSQAASHRVPPSRSTVERIGIAIG
                     QEAKRAAPKIEPYLRRSEQVPMGAVAVSVGLDRTTVPYEEEREEGAPPKTRRKKRTKP
                     YVRKAPPPVDVNYKMDYVGTVSLVDEQGEKLLSRKYFATHEDGPDGILKRMMADIRSA
                     LSQRPDLKVGVVQDGAPEMWNLVRSALKEELGVLTPLEGIDRYHLSERLGAVAKVIDR
                     DEGRRKQRLARWADMLDKDDGAIDEIERAIDGRSWAYEGKAQETLDETLTYIKNNKDR
                     MRYVSLREAGLPLGSGVTEGACKSLPARAKRSGQRWHDLGVSSVMELRAIDQSERLPR
                     FWRLLHRRYMARVESADGVMEHAA"
     gene            1700..>2026
                     /locus_tag="PKN52_06870"
     CDS             1700..>2026
                     /locus_tag="PKN52_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99705.1"
                     /translation="MLAPDLGVPWARDPSVLRALPGVEQAFENWATAREALLARGYAQ
                     TTLTNFEREGVRGTDRAFRYEECSFRPELHDALGFGPLSISTFIDPARRRAVKLIRGK
                     TFREEAA"
BASE COUNT          274 a          776 c          624 g          352 t
ORIGIN      
        1 ggcagcggcc cgaggaacgt cttgttcagg ctgggcgacc agtacttcgg gtctggaacg
       61 ctcccggatc acgcggcatg ctccatgacg ccatctgctg actccacgcg agccatgtag
      121 cggcgatgga gaaggcgcca gaacctcggc aggcgttcgc tctggtcgat cgcccgcagc
      181 tccatcaccg aggacacgcc caggtcatgc caccgctgcc cgctccgctt cgcccgcgcc
      241 ggcaggctct tgcacgcgcc ctcggtcacc ccgctgccca gcggcaaccc cgcctcgcgc
      301 aggctcacgt accgcatccg gtccttgttg ttcttgatgt aggtcaacgt ctcgtccagc
      361 gtctcctgcg ccttgccctc gtacgcccac gaccggccat cgatcgcccg ctcgatctca
      421 tcgatcgcgc cgtcgtcctt gtccagcatg tccgcccacc gcgcaagccg ttgcttccgc
      481 cttccctcgt ccctgtcgat caccttggcc acagcgccca gccgctcgga caagtggtag
      541 cggtcgatcc cctccagcgg ggtcagaacg cccagctcct ccttcagcgc agaacgcacc
      601 aagttccaca tctctggcgc cccgtcctgc accacgccca ccttcaaatc cggccgctgc
      661 gagagcgccg accggatgtc ggccatcatc cgcttgagga tgccgtcggg gccgtcctcg
      721 tgcgtcgcga agtacttgcg gctcaaaagc ttctccccct gctcgtccac gaggctgacc
      781 gtgccgacgt agtccatctt gtagttgacg tcgacgggcg gcggcgcctt gcgcacgtac
      841 ggcttcgtcc gcttcttgcg ccgcgtcttg ggcggcgcgc cttcctcgcg ctcctcctcg
      901 tacggcaccg tcgtgcggtc cagccccacc gagaccgcca ccgcgcccat aggcacctgc
      961 tcgcttcgcc gaaggtacgg ctcgatcttc ggcgccgccc gcttggcctc ctgcccgatc
     1021 gcgatgccga tccgctcgac ggtcgagcgc gagggtggaa cgcgatggct cgcggcctgc
     1081 gacttcacgt accttcgcga gttcacctcg ctccagtcca gcgcgatgct gtaggccagt
     1141 gccggggtcg cgtgatcgac caggccagcc tccagctcca gcggcaccac ggtcttggcg
     1201 ttgcgctcgc ccacgggacg atacgtcgct cgccctacgg ggaaggagcc gcagaggctg
     1261 tggtagccga cctcgccctt gtcgtggcag cggcggtgga gcacgccgtc gatgagcaac
     1321 tcgtcgccgt agccgtcgga catctgctgg agggggtctc ggaagtagca gcgcagcgcc
     1381 tcgttggagg cggccatcgc cgcgacctcg cgctgagaga acgtcgcgtt cggctccagc
     1441 tcggcctcga cgacgcggtg gagcgccctg acaaggtcgg ctctggcgac ctctgcctcg
     1501 ttgcccttgc ggttgctcgg tccctgatgc acgctgccca cgggtctcct ccaggcgtgg
     1561 tgtgtaagca gcgtcatgct ggatcggagg agacccacct tcaacccctc accccgtcgt
     1621 cgccggcctc aactcggtct cgccgtgctc cggcagcgat ccacaccccg gcctcgacca
     1681 gatctgcctc taccacctcg tgctggcgcc cgacctgggc gtgccgtggg cgcgcgaccc
     1741 gtcggtgctg cgcgcgctgc ccggcgtcga gcaggcattc gagaactggg cgacggcccg
     1801 cgaggcgctg ctcgcgcgcg ggtatgcgca gaccacgctg acgaacttcg agcgcgaggg
     1861 cgtgcggggc accgaccggg ccttccgcta cgaggagtgc tccttccgcc ccgaactcca
     1921 cgacgcgctc ggcttcgggc cgctctcgat ctcgaccttc atcgaccccg cgcgccgccg
     1981 ggcggtcaag ctcatccgcg gcaagacctt ccgcgaggag gccgcg
//
LOCUS       DAOQYE010000384         2025 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_64679_length_2025_cov_2.114213, whole genome shotgun sequence.
ACCESSION   DAOQYE010000384 DAOQYE010000000
VERSION     DAOQYE010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64679_length_2025_cov_2.114213"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(71..952)
                     /locus_tag="PKN52_06875"
     CDS             complement(71..952)
                     /locus_tag="PKN52_06875"
                     /inference="COORDINATES: protein motif:HMM:TIGR02276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99706.1"
                     /translation="MKHPKRLAAAIRAGSLSLIAGLSLVAGTALAQDSYRLYVANEFS
                     ADITVIDTASGEVIDTLAISGRPGEVRPRGMAVSPDGTIVYASVSDFNPILETPEDKI
                     LAIDVRTNEIVREFRAGGNPERVAVSPDGTQVWAALEAIAQGAGYDAITGEQLATFRV
                     GVEAEGVAVSPDGKWVYITAEATHTVTVVDREALKVVTHVLVGNRPRVVEFSNDGSRA
                     YVTAEIGGTVSVIDTSTHKVIDTINLGLDSRPVEIAVDPTSSLIYVAGGGTSGAPAAL
                     QRREATGCGLIRTAERP"
     gene            complement(1089..>2025)
                     /locus_tag="PKN52_06880"
     CDS             complement(1089..>2025)
                     /locus_tag="PKN52_06880"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNQ99707.1"
                     /translation="EAEALAAWSAEAGVPFINVGASADVLRNEQCRPTLFHVEPSAAM
                     YLDAMARWYVRSGLRDWYFLLDDTAESAAQLDRVKRTLDERHFAANIAGSTVIGSTTD
                     WDDVASRISRSRADFVILFMGADDQLAAVRELDVRGVSAMVTGYPYEDTQTRDFYRAW
                     RAAAPTLGAGHRISAWEATLDAYGARELNARYRMTYGEPMDTSAWAVYQGVKVLYEAA
                     FFGSSADPDDVMTYLTDLQSVFDVWKGIGTSFRPWDRQLRQPLYLVKIDQTEEAAFTL
                     ATLVGELPAIYMPGTEVAERLDQLGELAAASRCGR"
BASE COUNT          304 a          666 c          728 g          327 t
ORIGIN      
        1 gcttctggcc gggccccctg tccatcgtga ccggcgtcca tgcctccatc agtccgctgg
       61 cccgcgacgg tcagggacgt tcggccgtgc ggatgagccc gcaccctgtc gcctcacgcc
      121 tctgcagcgc cgccggcgcg ccactggtgc cgccgccggc cacgtagatg agggagctgg
      181 tcgggtcgac ggcgatctca acgggccgcg agtccaggcc gaggttgatc gtgtcgatga
      241 ccttgtgcgt gctcgtgtcg atgacggaga cggtgccgcc gatctcggcg gtcacgtagg
      301 ctctcgagcc gtcgttggag aactcgacga cgcggggtcg gttaccgacc agcacgtgcg
      361 tgacgacctt cagcgcctcg cggtccacga ccgtgaccgt gtgggtggcc tcggccgtga
      421 tgtagaccca cttgccgtcg gggctgaccg ccacgccctc ggcctcgacg ccgacgcgga
      481 acgtggcgag ctgttcacca gtgatggcgt cgtagccggc cccctgggcg atggcctcga
      541 gcgcggccca gacctgggtg ccgtcggggc tgaccgctac ccgctcgggg ttgccaccgg
      601 cgcggaactc gcggacgatc tcgttggtgc gaacgtcgat ggcgaggatc ttgtcctccg
      661 gcgtctccag gatggggttg aagtcgctga cggaggcgta gacgatcgtg ccgtccgggc
      721 tgaccgccat gccgcgcggc cggacctcgc ctggtctgcc ggagatggcc agcgtgtcga
      781 tgacttcgcc gctcgcggtg tcgatgaccg tgatgtcggc gctgaactcg ttggccacgt
      841 agaggcggta cgagtcctgg gccagcgccg taccggcaac cagagacaac ccggcgatca
      901 gggaaagcga acctgcgcgg atagcggcgg cgagacgttt cgggtgcttc atggtctcct
      961 tccgctcgcg caaacgaacg cgagcctact gctaacgcat cgagcgaccg gaccaacgcg
     1021 aggaaccgtc gttgacgtgc cgcaccacgt tatcgccaac gtgtaaggcg aacgtaagta
     1081 gggcatcgtc acctgccgca gcggctagcc gccgcgagtt cgccgagttg gtcgagccgt
     1141 tcggcgacct cggtgccggg catgtagatg gccggcagct cgccaacgag ggtggcaagg
     1201 gtgaacgcgg cctcctccgt ctggtcgatc ttcacgaggt acaggggctg gcggagctgc
     1261 cggtcccacg gcctgaagct cgtgccgatc cctttccaga cgtcgaacac ggactggagg
     1321 tcggtcaggt aggtcatgac gtcgtcggga tcggcggagc tgccgaagaa ggccgcctcg
     1381 tagaggacct tcacgccctg gtagacggcc caggcgctcg tgtccatcgg ctcgccgtac
     1441 gtcatgcggt agcgggcgtt gagctcgcgc gcgccgtagg cgtcgagcgt cgcctcccac
     1501 gccgagatgc ggtggccggc gccgagcgtc ggggcggcag cgcgccacgc ccggtagaag
     1561 tccctcgtct gggtgtcctc gtaggggtag ccggtgacca tggcggagac gccgcgcacg
     1621 tccagctcgc gcacggcggc gagttgatca tccgcgccca tgaacaggat gacgaagtcg
     1681 gcacgcgagc gcgagatgcg cgaggcgacg tcgtcccagt ccgtggtcga cccgatcacg
     1741 gtgctgcccg cgatgttggc cgcgaagtgg cgctcgtcga gggtgcgctt gacgcggtca
     1801 agttgggccg ccgactcggc ggtgtcgtcc agcaggaagt accagtcgcg caagccagag
     1861 cgcacgtacc agcgggccat ggcgtccagg tacatggccg cgctcggctc cacgtggaag
     1921 agcgtgggcc tgcactgctc gttgcggagc acgtcggccg aggcgccgac gttgatgaag
     1981 ggcacgccgg cctctgccga ccaggcggcc aaagcctccg cttcg
//
LOCUS       DAOQYE010000385         2023 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_64769_length_2023_cov_3.030996, whole genome shotgun sequence.
ACCESSION   DAOQYE010000385 DAOQYE010000000
VERSION     DAOQYE010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64769_length_2023_cov_3.030996"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..783
                     /locus_tag="PKN52_06885"
     CDS             <1..783
                     /locus_tag="PKN52_06885"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99708.1"
                     /translation="RLALAGVHPDLKEVGPATTTEAGRAKRNLEIRIDQLVPREGGDP
                     EPLAPWLLTRPRYRARVGLIDHAEALNASAANAFLKVLEEPPSWALIVLVAAGPEALL
                     PTVASRCVTVRFAAVDAVLARLGEAGAPLPPDVLAGHPAVRLGQPGALLRAAEDVDAT
                     TAARLAMEGFLAGLAGDMLTAMEAAETLAKAVTANMEAGNEPGPLGWLREALRSLPAG
                     SYAAALDAIDDCERAVAAYAQVPLACSVLALRLRGIGVGSGG"
     gene            complement(797..1594)
                     /gene="ricT"
                     /locus_tag="PKN52_06890"
     CDS             complement(797..1594)
                     /gene="ricT"
                     /locus_tag="PKN52_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HNQ99709.1"
                     /translation="MPECIGIKFDNGPKIHYLEAPPSAPSVGTRCVVTTRRGLELGRV
                     RTSVCEVAKPQGHYVRDAVEEDLQRFEELKAKAEDLKWLLKARARQVDPDVKIVALEF
                     NIDETLLVVSYTSERQAPLRVLAQELVEHTEARIEFANVGPRDQARMLGALGVCGDGT
                     CSSTWLQSFNAVSIRMARDQQLPLNPEKISGPCGRLMCCLQYEHDMYRDLLKGLPRKG
                     AKACHDGSGTCGRVVKLNPLKGTVELRTDEGGFGEYPASEITRMRGA"
     gene            complement(1688..>2023)
                     /locus_tag="PKN52_06895"
     CDS             complement(1688..>2023)
                     /locus_tag="PKN52_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99710.1"
                     /translation="LRAVAALAVAPDALVTDYLKLPTALPTVAVAAADAKSYQVAAAS
                     ILAKTTRDAHMAELARRYPGYGLEKHKGYGVASHLRALALRGPTPEHRRSFAPVAQPR
                     LLEADAACR"
BASE COUNT          260 a          713 c          729 g          321 t
ORIGIN      
        1 cggctggcgc tggcgggggt gcacccggac ctcaaggagg tcgggccggc gaccacgacg
       61 gaggcgggcc gcgcgaaacg caacctcgag atcaggatcg accagctggt gccgcgcgag
      121 ggcggcgacc ccgagccgct cgcgccatgg ctcctgaccc gcccgcgcta ccgggcacgc
      181 gtcggcctga tcgaccatgc cgaggccctc aacgcgtcgg ccgccaacgc gttcctcaag
      241 gtcctcgagg agccgccgtc gtgggcgctc atcgtactcg tggcggccgg gccggaggcg
      301 ctcctcccca ccgtggcgtc gcgctgcgtg accgtgcggt tcgcggccgt cgacgccgtg
      361 ctcgcgcgac tgggcgaggc gggggcgccg ctcccgccgg acgtgttggc cggccaccca
      421 gccgtccgcc ttgggcaacc gggggcgctc ctgcgcgctg ccgaggacgt cgacgccact
      481 accgcggcca ggctcgcgat ggaagggttc ctggcgggtc tcgccggcga catgttgacc
      541 gctatggagg ccgccgagac gctcgccaag gccgtgacgg cgaacatgga agccggaaac
      601 gagcccggtc cgctcggctg gctgcgtgag gctctccggt cgttgccggc agggtcctac
      661 gccgcggcgc tagatgcgat cgacgactgc gaacgcgcgg tcgcggcgta cgcccaagtg
      721 ccgctcgcct gttcggtgct ggctctcagg ctgcgcggca tcggagtcgg ctcgggcggc
      781 tgacgcgacc ggcggctcag gcgccgcgca tgcgcgtgat ctcgctcgcg ggatactcac
      841 cgaagccgcc ctcgtcggtc cgcagctcca ccgtgccctt gagcgggttg agcttgacga
      901 cgcggccgca cgtgccgctg ccgtcgtggc aagccttcgc gcccttacga ggtaagccct
      961 tcagaaggtc cctgtacatg tcgtgctcgt actggaggca gcacatcaac cgaccgcacg
     1021 gtccgcttat cttctccggg ttcaggggga gctgttggtc gcgcgccatc ctgatgctca
     1081 cggcgttgaa gctctgcagc caagtgctcg agcaggtccc gtcgccgcag acgcccaggg
     1141 cgcccagcat ccgggcctgg tcgcgcgggc cgacgttagc gaactcgatg cgcgcctcgg
     1201 tgtgctcgac gagctcctgc gcgagcacgc gcaggggcgc ctgccgctcg ctcgtgtagc
     1261 tgaccactag gagcgtctcg tcgatgttga actccaaggc cacgatcttc acgtccgggt
     1321 cgacctggcg ggcccgcgcc ttgaggagcc acttgaggtc ctcggccttg gccttcaact
     1381 cctcgaagcg ctgcaggtcc tcctcgacgg cgtccctcac gtagtgaccc tggggcttgg
     1441 cgacctcgca cacgctcgtg cgcacccggc cgagttccag gccgcgtcgc gtcgtgacga
     1501 cgcaacgcgt gcctacggac ggggcgctcg gcggcgcctc gaggtagtgg atcttcggac
     1561 cgttgtcgaa cttgataccg atgcactccg gcatggtgaa ggctccttct gacacccctt
     1621 ggcgcgtcgg cttgctcggg gcctcaggca gcgcgggacg cccatcgggg ttcggccatt
     1681 ctacgcgtca gcggcacgcc gcgtcggctt cgaggagcct tggctgcgcg acgggggcga
     1741 aactccgccg atgctcgggg gtcggtccac gcagcgcaag ggcgcgcagg tggctcgcca
     1801 ccccgtagcc cttgtgcttc tcgagtccgt acccggggta gcgcctcgcc agctcggcca
     1861 tgtgggcgtc gcgtgtcgtc ttggcgagga tgctggcggc ggccacctgg tagctcttgg
     1921 cgtcggccgc ggccacggcc acggtcggca gggcggtcgg gagcttgaga tagtcggtga
     1981 ccaaggcgtc cggtgcgacc gcgagggcgg cgacggcgcg cag
//
LOCUS       DAOQYE010000386         2023 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_64783_length_2023_cov_2.618394, whole genome shotgun sequence.
ACCESSION   DAOQYE010000386 DAOQYE010000000
VERSION     DAOQYE010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64783_length_2023_cov_2.618394"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="PKN52_06900"
     CDS             complement(<1..391)
                     /locus_tag="PKN52_06900"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNQ99711.1"
                     /translation="MPKVFLIMLDGVGADTFRTYRSRLPFLSHLAAGGYGVERLRSLV
                     PGISLPGRTSILTGRSAADAGVYGNNVWHDTRFSYADPDDVRVPTLPRLALAAGLDVA
                     CIGFGMVRPEDATVFKRPWWADDFVQRA"
     gene            764..>2023
                     /locus_tag="PKN52_06905"
     CDS             764..>2023
                     /locus_tag="PKN52_06905"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNQ99712.1"
                     /translation="MLALSFMFAAAQEGTDGKGLYDANCAVCHGATGQGSVGPALAGN
                     SNLTDPDYHIRQILRGGNSMPSFAKSLSDDDIAAIATFERTSWGNDFDPITSEQVAAV
                     RAGGSASSVKADDTTAAQVSAAEPVVVTADFAVEPARLEDGNGMTLYIFHNDSLSGTS
                     RCAAPCTTHWTPLIAAGAITAGDGIDAADLGTIARGDGTSQVTYRGWPLYLSAADSAP
                     GDTAGAGTSAMWDIATPGMAVIALSMTPTTVPAAAEGGGEAGAPATDNPPVTPIVYQG
                     TYEPVTNERLLDPAPGDWLMYKRTYSLQAYSPLDQINVDNVADLVPVWSFGTGVSDGH
                     EAPPIVNGGLMFATAPFSTLFALDVRTGDLVWQYKRELPSDVAPEVCCGMVNRGVALY
                     GDKVYLATLDAHLLAFEATTGELVWDTT"
BASE COUNT          368 a          695 c          654 g          306 t
ORIGIN      
        1 gcgcccgctg aacgaagtcg tccgcccacc acggccgctt gaagacggtc gcgtcctccg
       61 gccgcaccat gccgaagccg atgcacgcca catcaaggcc cgccgcgagc gccaaccggg
      121 gcaacgtcgg cacccgcacg tcatcggggt cggcgtaact gaagcgcgtg tcgtgccata
      181 cgttgttgcc gtacacgccg gcgtcggccg cgctgcgacc cgtgaggatc gacgtccgcc
      241 ccggcagcga gataccgggg acgagggacc gtaggcgctc gacaccgtag ccgcctgcgg
      301 ccaggtgcga gaggaacggc aggcggcttc ggtaggtacg gaaagtgtcc gcgccgacac
      361 cgtcgagcat gatgaggaag acctttggca tgggccagtg taacgacctg gcagacccag
      421 gcccatgcgc ccctcaagcg caggaaggcg tgccacacac cagatgggcg acccagatgg
      481 gcgaccgcga cgccgccggc gagccgcagg ggcgcagggg cgcaggatcc ttataccgga
      541 gtcagaacat ccctcggcgg tattcatcag catggtcagg cacactagat cctgactcgg
      601 tcatacttcg tgctaccatg gcgcccgtca gatgccaact ctgttcaacg tgacaattcc
      661 atggcatctc tggctcgtcg cgggaactcg cccaggagtc cccgatcggg tcctgctccg
      721 gaggtaatcc gtaatgagac cagcacggtg gctattcacc tcggtgctcg cactgagctt
      781 catgttcgcc gcagcgcagg aagggacgga cggcaagggt ctttacgacg ccaactgcgc
      841 ggtctgccac ggcgccaccg gtcaaggcag cgtagggccc gccctcgccg ggaactcgaa
      901 cctgacggac cccgactatc acatccggca gatcctgcgc ggcggcaact cgatgccgag
      961 cttcgcgaag tccttgagcg atgacgatat cgcggcgatc gccaccttcg agcgcacctc
     1021 atgggggaac gacttcgacc cgatcacgtc cgagcaggta gcggccgtca gggcgggcgg
     1081 ctctgccagt tccgtcaaag cagacgacac gacggccgcg caggtctccg cggcagagcc
     1141 tgtcgtggtc acggccgatt tcgccgtaga gcctgctcgg ctagaagatg gcaacggcat
     1201 gacgctctac atcttccata acgattcgct gtccggcacc agtcggtgcg cggcgccgtg
     1261 caccactcac tggaccccgc tcatcgcggc gggcgccata acggccggcg atgggataga
     1321 cgccgccgac ctcggcacca tcgcgcgtgg cgacggcaca tctcaggtga cgtatcgcgg
     1381 ctggccgctc tacctgtcag ccgcagattc cgctccgggc gacacggcag gtgccggcac
     1441 ctccgccatg tgggacatcg ctacgcccgg catggctgtg atcgcgttga gcatgacgcc
     1501 gaccacggtg cccgctgcag cggaaggcgg gggcgaggcc ggagcaccgg cgaccgacaa
     1561 cccgccggtc acgcccatcg tttaccaagg cacttacgag cccgtaacga acgagcggct
     1621 cctcgacccg gcccccggcg actggctcat gtacaagcgc acgtactcac tgcaggcgta
     1681 cagcccgctc gaccagatca acgtggacaa cgtggccgac ctggtcccgg tatggtcgtt
     1741 cggcaccggc gtgtcagacg ggcatgaggc gccgcccatc gtgaacggcg ggctcatgtt
     1801 cgccacggcg ccgttcagca cgttgttcgc cctcgatgtc cgcacgggtg acctcgtctg
     1861 gcagtacaag cgcgagctgc cgagcgacgt tgcgcccgaa gtgtgctgcg ggatggtcaa
     1921 ccgtggtgtg gcgctctacg gcgacaaggt ctacttggca acgctggacg cccacctcct
     1981 cgccttcgag gccacgacgg gcgaactcgt atgggacacc acg
//
LOCUS       DAOQYE010000387         2023 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_64784_length_2023_cov_2.617378, whole genome shotgun sequence.
ACCESSION   DAOQYE010000387 DAOQYE010000000
VERSION     DAOQYE010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64784_length_2023_cov_2.617378"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="PKN52_06910"
     CDS             complement(<1..324)
                     /locus_tag="PKN52_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018113653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99713.1"
                     /translation="MQVMKSCPAPAKSAPAPKASKAPTKIVSTYDYRNEQGELLMQVV
                     RMEPKTFRQRRPDGERWSWSVKDVRVVPYRLPELAAAPDAVVYLVEGEKDADRLASLD
                     LVATCN"
     gene            456..761
                     /locus_tag="PKN52_06915"
     CDS             456..761
                     /locus_tag="PKN52_06915"
                     /inference="COORDINATES: protein motif:HMM:NF024672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HNQ99714.1"
                     /translation="MTVSRASDEGGPAGEAPAGQLRVDETREEAPAMRLDDLLKFFGV
                     AGTRGHAKHLIQSGAVAVNGVTETRRKHLIRAGDTVAVGGEAFVVEFERTPEPDAPT"
     gene            complement(758..1309)
                     /locus_tag="PKN52_06920"
     CDS             complement(758..1309)
                     /locus_tag="PKN52_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99715.1"
                     /translation="MREGLEDALLTCTAAGSENGPVEGTPYIGRVEVAALPLLRLGPE
                     LVLRPELALLARHRRWGPGPAPGELVSAGAGLAAEGVVLVVELGAHGQSEARRPERGG
                     PGQRSASLEGRLSAYAVAGVREVWSLDVDRTWTVAYRSPWGGAYRSRTLWYPGEAVPV
                     TGLNNVAVETFADAAPAGRRAGG"
     gene            1825..>2023
                     /locus_tag="PKN52_06925"
     CDS             1825..>2023
                     /locus_tag="PKN52_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99716.1"
                     /translation="MPRREDIHKILILGSGPIVIGQAAEFDYSGTQACKALRGAGFEV
                     VLVNSNPATIMTDPEVADRTYI"
BASE COUNT          299 a          744 c          681 g          299 t
ORIGIN      
        1 attgcaggtt gccaccaagt ccagagatgc aaggcggtct gcgtcctttt ctccttccac
       61 caggtacacc accgcatcgg gcgctgccgc cagctcaggc aggcggtagg gcacaacgcg
      121 cacgtccttg acgctccagc tccagcgctc gccatcgggg cgacgctgac ggaatgtctt
      181 gggctccatg cgaacgacct gcatcagcag ttcgccctgt tcattgcggt agtcgtaggt
      241 gcttacgatc ttggtcgggg ctttgctggc cttgggcgca ggcgcactct tggctggcgc
      301 cgggcatgac ttcatgactt gcatgacttc gcgcccggct ggagtgctgg cgcggtcatt
      361 gccgatgaat ccgttttgga ccagccactg atgcgccgcg ccgcgttcgt caagttcctc
      421 ggcagctacc cggccgcgga gcctatcgag gtcaggtgac cgtgagccgc gcttccgacg
      481 agggcggccc ggcgggcgag gcgcccgcgg gtcagctgcg cgttgacgag acccgtgagg
      541 aggcgccggc catgcgcctc gacgacctac tcaagttctt cggggtcgcc gggacacgcg
      601 gtcatgccaa gcacctcatc cagtcgggcg ccgtggcggt caacggcgtg accgagaccc
      661 gccgcaagca cttgatcagg gcgggtgaca cggtcgcggt cggcggggag gcgttcgtgg
      721 tcgagttcga gcggacaccg gagcccgacg cccccaccta gccgccggcc cggcgtcccg
      781 ccggcgcggc gtccgcgaac gtctccaccg ccacgttgtt gagaccggtg accggcaccg
      841 cctcgcccgg gtaccagagc gtgcgcgagc ggtaggcgcc accccacggc gagcggtacg
      901 ccaccgtcca cgtcctgtcg acgtcaagcg accacacctc gcgtacgccc gcgacggcgt
      961 acgcgctcag gcgtccctcc aacgaagccg acctctggcc cggaccaccg cgttcagggc
     1021 gccgggcctc gctctgacca tgggccccaa gctcgacgac gaggacaacc ccttccgccg
     1081 cgagtcccgc gccggcgctc accaactcgc ctggcgccgg ccccggtccc caccggcggt
     1141 gacgcgcgag cagcgccagc tcgggtctga gcacgagttc cggtccgagc ctcagcagcg
     1201 gcagcgcagc cacttcgacg cgaccgatgt acggggtgcc ttcgaccggc ccgttctcac
     1261 tgccggccgc cgtacacgtc agcagggcgt cctccaggcc ctcccgcaag gccgcaagga
     1321 gggaggcctc cgcggaccgg cgcggcggcc tcgccaccac ggcgccgaac cagagctcgg
     1381 acctggcggc gtcggctacg ccgccctccc gcagatggga gaggaacccg gccgcggcga
     1441 gcttcggttc cggcgccccg gcgtgccaac ggccccgtcc ggcccgacca taagcgggcg
     1501 ggtgaccgga accgggcgcg ccgctttctg gtggcgctcc actttgtggt ggcgctccaa
     1561 cgggagcggg cgcgccaccg ggaggtgacg gcaacgaccg gtcggtccgg gcgccgtgcc
     1621 cccgcccgcc ggcgagcggc gacgctgcat caccttcggt tcgtcctcgt gccatgcggc
     1681 caagaatcgc aacgtacgca acttcatgca gcgggggcgc gggcagtcgc cgcagggtgc
     1741 taggcgcgca ccctggtcca gggtgcgcgt cgcctccggc atggtggtac actccgcctt
     1801 tggtgattgc gaggccccca ctccatgcct agacgcgaag atatacataa gatcctcatc
     1861 ctcggctcgg gcccgatagt gatcggccag gcggccgagt tcgactactc cggcacccag
     1921 gcatgcaagg cgctgcgcgg ggccggcttc gaggtcgtgc tcgtcaactc caacccggcc
     1981 acgatcatga cggaccccga ggtggccgac cgtacctaca tcg
//
LOCUS       DAOQYE010000388         2021 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_64898_length_2021_cov_2.708037, whole genome shotgun sequence.
ACCESSION   DAOQYE010000388 DAOQYE010000000
VERSION     DAOQYE010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64898_length_2021_cov_2.708037"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="PKN52_06930"
     CDS             <1..356
                     /locus_tag="PKN52_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99717.1"
                     /translation="FEALDRARLSLTGELTNPGLIGYLAAVPQATLSVGGAAAATIAL
                     APLQVPGGGTVPVVLEFTFAPLTLGAAIAAQVQAASVGAGGLSLQLAGAWRLEAPGIA
                     TMTLGATTFLRDALR"
     gene            complement(383..>2021)
                     /locus_tag="PKN52_06935"
     CDS             complement(383..>2021)
                     /locus_tag="PKN52_06935"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HNQ99718.1"
                     /translation="ARPIRALLLLMGLVSLVGMPYAVLMPIFADRILHGGPKALGLLM
                     GAAGIDYALLIVNRFREELRSTFDTRKAAARTAETAGKAVAFSGLTVIVGLMALLVPP
                     VAFIRSIGVGAMVVLCVSVLVAITAVPATLALLGHRVNWLRVTKREPGLRSRAFWRAQ
                     ATRIMRRPRFWFVTGVLVLVGLALPSLRMQVADPGARGLTSSTDARRTLDALEGLGLE
                     GLLNPFDVVVDFGEEGFYRPANVRKVSLLTQRARELAHVAGVSSPMALESIPKLFLYQ
                     YYATREVALASEIAPLARATVSRDGRYALIKLVPVGALTPAQGAEVHGALEVALLELG
                     LRGSIGGVYVQGTEWTRALYAYFPLALALVAITTALLLGMAFKSLLIPIKAVLLNALT
                     VCAAFGVMTLVHQDGIIHRLFGLGEVLGYIDTSAPLFIFAIVFGLSMDYEVFMVARIH
                     EAHERGMTDHDAVATAISTTGGVITSAAAVMVTVFALLTFSHVELIRTLGLGLSVAVI
                     LDATLVRLALVPSVMALAGGWNWWLPRFRARQPDGRK"
BASE COUNT          337 a          707 c          715 g          262 t
ORIGIN      
        1 ggttcgaggc gctcgaccgc gcgcggctca gcctaaccgg cgagctgacg aacccgggcc
       61 tgatcggcta cctggcggcg gtgccgcaag ccacgctgtc ggtcggtggc gccgcggcgg
      121 cgaccatcgc cctcgcgccc ctccaagtgc ccggaggcgg gaccgtgccc gtcgtcttgg
      181 agttcacgtt cgccccgctg acgcttggcg ctgccatcgc cgcacaggtg caggccgcct
      241 cggtcggagc cggcgggctc agcctgcaac tggccggcgc ctggcgccta gaagcgcccg
      301 gtatcgcgac catgacgctc ggggcgacga cgttcctccg cgacgcgttg cgctagacga
      361 gcgcacctcc gccccggcgt ccctacttgc gcccgtccgg ctgccgcgcc ctgaagcgcg
      421 gcaaccacca gttccatccg cccgccagcg ccatcacgga gggcaccagc gcgagccgca
      481 cgagggtggc gtccagtatc accgccacgc tgaggccgag gccgagcgtg cggatcagtt
      541 cgacgtgcga gaaggtgagc agcgcgaaca ccgtcaccat gacggccgcg gcgctggtta
      601 tgactccacc cgtcgtcgag atggcggtgg cgaccgcgtc atgatccgtc atgccgcgct
      661 cgtgcgcctc gtggatgcgc gcgaccatga acacctcgta gtccatgctc aggccgaaga
      721 cgatggcgaa gatgaacagg ggtgccgacg tgtcgatgta cccgaggacc tcgcccaagc
      781 cgaagagtcg atggatgatc ccgtcctggt ggacgagcgt catgaccccg aaagcggcgc
      841 atacggtcag cgcgttgagc agcacggcct tgatcggtat gagcagggac ttgaaggcca
      901 tgccgagcag cagcgcggtg gtgatggcca cgagcgctag cgccaacggg aagtaggcgt
      961 agagcgcgcg cgtccactcc gtcccctgga catacacccc gcctatcgag ccgcgcaggc
     1021 caagttcgag gagcgcgacc tcgagcgcgc cgtggacctc cgcgccctgc gccggcgtca
     1081 gggcgccgac cggcacgagc ttgatcagcg cgtagcggcc gtcgcgcgac accgtcgccc
     1141 gcgcgagcgg ggcgatctcg ctggccaacg ccacctcccg ggtcgcgtag tactggtaca
     1201 ggaagagctt ggggatgctc tcgagcgcca tcggagacga gacgccggcc acgtgcgcga
     1261 gctcgcgcgc ccgctgcgtg aggagcgaga ccttgcgcac gttcgcgggg cggtagaagc
     1321 cttcctcgcc gaagtcgacg acgacgtcga acgggttgag aagcccctcg aggcccaagc
     1381 cttcgagggc gtcgagggtt cggcgcgcat cggtgctcga ggtcagtccg cgggcgcccg
     1441 gatcagccac ctgcatcctg agcgacggga gcgccaggcc cacgagcacc aacacgcccg
     1501 tgacgaacca gaagcgtggg cgacgcatga tgcgtgtggc ctgggcgcgc cagaacgccc
     1561 gactgcgcag gcccggctcg cgcttcgtca cgcgcagcca gttgacccgg tggccgagga
     1621 gcgccagggt ggccggcacg gccgtgatcg ccacgagcac gctcacgcat agcacgacca
     1681 tggcgccgac gccgatggag cggatgaagg cgacgggcgg gacgaggagc gccatgaggc
     1741 cgacgatgac ggtgagcccg ctgaaggcga cggccttgcc ggccgtctcg gccgtgcggg
     1801 cggcggcctt gcgcgtgtcg aacgtgctgc gcagctcctc cctgaagcga ttgacgatga
     1861 gcagggcgta gtcgatgccc gcggcgccca tcagcagccc aagggccttc ggcccgccat
     1921 gcaggatacg gtcggcaaaa atcggcatga gcaccgcgta gggcatgccc accaggctga
     1981 ccagccccat cagcagcagc agcgcgcgaa tcggccgggc c
//
LOCUS       DAOQYE010000389         2020 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_64932_length_2020_cov_2.715522, whole genome shotgun sequence.
ACCESSION   DAOQYE010000389 DAOQYE010000000
VERSION     DAOQYE010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64932_length_2020_cov_2.715522"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..604
                     /locus_tag="PKN52_06940"
     CDS             <1..604
                     /locus_tag="PKN52_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012536130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HNQ99719.1"
                     /translation="DNDKPTVTLVHKGNIMKFTEGAFRDWGYELADREFGATLLDGGP
                     WRTLKNPRTGRNIVINDVIADAMLQQVLTRASEYSVIATLNLNGDYLSDALAAQVGGI
                     GIAPGANINYLTGHAVFEATHGTAPKYAGQDKVNPSSVILSGEMLLRHLGWNEAADMV
                     LRSVSQTIGQKRVTYDFHRLMEGATLLKCSEFGEALIENL"
     gene            complement(677..1150)
                     /locus_tag="PKN52_06945"
     CDS             complement(677..1150)
                     /locus_tag="PKN52_06945"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNQ99720.1"
                     /translation="MARREFVVAAAILLDPSGRVLLVGNDWQGHGRVRYTLPGGVVER
                     GESTLDALVREVKEETGLEITGVKGLAYAVHVEDRRRNDRAVSFAFLAEYQGLLNPRD
                     PDGFIVEARFFPVEEVAMIVPIPPLRDPLVTYLRERVPGRFYAYGGWDGRALQSA"
     gene            complement(1137..>2020)
                     /locus_tag="PKN52_06950"
     CDS             complement(1137..>2020)
                     /locus_tag="PKN52_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015760833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HNQ99721.1"
                     /translation="ILPADARSHKKLAELLTPRDPFDDKDVIVEIRAAAGGDEASLFA
                     REMLGMYLRYAGDLGFKTELLDSHPTEVGGLSKVSFAVTGAGVYRAFKFESGVHRVQR
                     VPATEAQGRIHTSTVTVAVLPEADEVDVALSPADYRVDVYRSSGPGGQSVNTTDSAVR
                     VTYHPGEPDEIIVTCQDGKSQIKNKERALTVLRSRLLERERERAMSEAREARLVQIGT
                     GDRSERVRTYNFPQSRVTDHRIDFTTHGLAGVLAGGLEELTAALQAEERARLALEAAH
                     AGGDALAERLGGARGAA"
BASE COUNT          314 a          724 c          682 g          300 t
ORIGIN      
        1 cgacaacgac aagccgacgg tgacgctcgt tcacaagggc aacatcatga agttcacgga
       61 gggcgcgttc cgcgactggg gctacgagct cgccgaccgc gagttcgggg cgaccctcct
      121 ggacggcggg ccgtggcgca ccctcaagaa cccccgcacg ggccggaaca tcgtcatcaa
      181 cgacgtcatc gccgacgcca tgctgcagca ggtcctcaca cgcgcgagcg agtactctgt
      241 catcgccacc ctcaacctca acggcgacta cctctccgac gcgctcgccg ctcaggtcgg
      301 cggcatcggc atagctcctg gcgcgaacat caactacctc accggtcacg ccgtcttcga
      361 ggccacccac ggcacggcgc ccaagtacgc cggccaggac aaggtgaacc cgtcctccgt
      421 catcctctct ggcgagatgc tcctgcgtca ccttggctgg aacgaggcgg ccgacatggt
      481 cctccgctcc gtcagccaga cgatcggcca gaagcgcgtc acgtacgact tccaccggct
      541 catggaaggc gccacgctcc tcaagtgcag cgagttcggc gaggcgctca tagagaacct
      601 ctgagacccg ggtcgctaag gacagggcgg cggaagcttc tagttccgcc gccccgtctc
      661 gttgcttccc ggtcggtcag gcgctctgca gcgcccgccc gtcccagccg ccgtaggcgt
      721 agaagcgccc cggcacgcgc tcgcgcaggt aagtgacgag cgggtcgcgt agcggcggta
      781 tcgggacgat catcgcgacc tcctcgaccg ggaagaagcg cgcctcgacg atgaacccgt
      841 ccgggtcgcg cgggttgagg agcccctggt actcggcgag gaacgcgaac gacacggcgc
      901 ggtcgttgcg ccgcctgtcc tcgacgtgga cggcgtacgc caggcccttg acgcccgtta
      961 tctcgaggcc cgtctcctcc ttgacctccc ggacgagcgc gtcgagggtg gactcgccgc
     1021 gctcgaccac gccgccgggc agcgtgtaac ggacccggcc gtgcccttgc cagtcgttgc
     1081 ctacgagcag tacccgaccc gaggggtcga gcaggatggc cgcggcaacg acgaactcac
     1141 gccgcgccac gagcgccgcc cagccgttcg gcgagggcgt ccccacccgc gtgcgcggcc
     1201 tcgagagcga gcctggcccg ctcctcggcc tggagggcgg ccgtgagctc ctcgaggccg
     1261 ccggccagca cacccgcgag gccgtgggtg gtgaagtcga tgcggtggtc ggtgacgcgc
     1321 gactgcggga agttgtacgt ccgcacccgc tcggaccggt cgccggtgcc gatctgcacg
     1381 agccgcgcct cgcgcgcctc gctcatggcg cgctcgcgct cgcgctcgag cagccgcgag
     1441 cgcaggaccg tgagggcgcg ctccttgttc ttgatctgcg acttgccgtc ttggcacgtc
     1501 acgatgatct cgtcgggctc gccggggtga taggtgaccc tgacggccga gtcggtcgtg
     1561 ttcacggact gcccgcccgg tccgctggag cggtagacgt ccacccggta gtcggcgggg
     1621 gagagcgcga cgtccacctc gtcggcctcg gggagtacgg ccaccgtgac ggtgctcgta
     1681 tggatgcgcc cctgcgcctc ggtggcgggc acgcgctgca cgcggtgcac gcccgactcg
     1741 aacttgaacg cgcggtaaac gccggctccg gtgacggcga aggagacctt cgacagcccg
     1801 cccacctcgg tggggtggga gtcgagcaac tcggtcttga aacccaggtc gcccgcgtag
     1861 cgcaagtaca tgccgagcat ctcgcgggcg aacagagagg cctcgtcgcc gcccgccgcc
     1921 gctcggatct cgacgatgac gtccttgtcg tcgaacgggt cgcggggcgt gagcagctcg
     1981 gccagcttct tatgactgcg ggcgtcggca ggcaggatcc
//
LOCUS       DAOQYE010000390         2018 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_65016_length_2018_cov_3.990830, whole genome shotgun sequence.
ACCESSION   DAOQYE010000390 DAOQYE010000000
VERSION     DAOQYE010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65016_length_2018_cov_3.990830"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="PKN52_06955"
     CDS             <1..271
                     /locus_tag="PKN52_06955"
                     /inference="COORDINATES: protein motif:HMM:NF037016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNQ99722.1"
                     /translation="GVSTQNIDIVVRGDTAVEPDAQLIITLTGPVDCVLLVQPAGGTI
                     TQVTDAWAVTYAPRTGFTGSDTFEYTVRDAPNASRTGFITVTVTT"
     gene            283..1593
                     /locus_tag="PKN52_06960"
     CDS             283..1593
                     /locus_tag="PKN52_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99723.1"
                     /translation="MPEPNLNAARRKAEAMGRQAAQLGQRVAETKAAQEARLAELQAA
                     QKANLDQMVANQAEAVAAMVAAQSTRMDELATRTGDLEGMAGEIASLLTAPPEPPPPV
                     RDLFLEPFNEQSAHRARIPPDVEYGIPPGTLNAKVAKPVYDAGKKGERGRLALVRTFR
                     CGATGQGRKYLIKVSPNDPPRRVVWHKAGGSGDGLPITVRMPAFRPGLYPTGDGDNSV
                     LLYDPAADRVYQFNQFSYGTTEATARACFSYPLSGRDVRNRWDDPGMWGPGAIDWRHP
                     GGFLRGREVDLTGAVQIRHVLNATATRHSGAGATAAQHALSKRMAYPAWETDRPLDAN
                     DNLGDLPYGTIVAIRRQDAGLRETLGLSPFGKRLFDTLLAGEGYGCCIGDGQGQIVDN
                     APVLQLRVDSELAENAAKVRAVDEALAKLLPHLWPVFTTRRHDA"
     gene            1603..1740
                     /locus_tag="PKN52_06965"
     CDS             1603..1740
                     /locus_tag="PKN52_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99724.1"
                     /translation="MDWLLIALVVALLVGAVLWMLRGPGDGDDDTTPQRWGGGGGRGR
                     R"
BASE COUNT          299 a          741 c          701 g          277 t
ORIGIN      
        1 cggcgtctcc acccagaaca tcgacatcgt ggtccgtggc gacaccgcgg tcgagcccga
       61 cgcgcagctt atcatcactc tcaccgggcc ggttgactgc gtgctcctgg tgcagccggc
      121 gggcgggacc atcacccagg tcaccgacgc ctgggcggtc acctacgcgc cccggaccgg
      181 cttcaccggc agcgacacgt tcgaatacac ggtccgcgac gcgccgaacg ccagtcgcac
      241 cggcttcatc accgtgaccg tgaccacctg acggagcgac cgatgcccga gccgaacctc
      301 aacgccgccc gccgcaaggc cgaagccatg ggccgccagg cagcccagct cggccagcgg
      361 gtcgccgaga ccaaggcagc ccaggaagcg cggctggcgg agctccaggc cgcgcagaag
      421 gcgaacctgg accagatggt ggccaaccag gccgaggccg tcgccgccat ggtcgcggcg
      481 caaagcacgc gcatggacga gctcgccacc aggaccggcg acctggaagg catggccggc
      541 gagatcgcgt cgctgctgac cgctccgccc gagccaccgc cgccggtccg tgacctgttc
      601 ctcgagccgt tcaatgaaca gagcgcgcac cgcgcccgga tcccgcccga cgtcgagtac
      661 ggcatcccgc cgggcacgct gaacgccaag gtcgccaagc cggtctacga tgccggcaag
      721 aagggcgagc gcgggcggct ggcgctggtc aggacgttcc gctgcggcgc caccggccag
      781 ggccggaagt acctcatcaa ggtctcgccc aacgatccgc cgcggcgcgt cgtgtggcac
      841 aaggccggcg gcagcggcga cgggctcccc atcacggtcc gcatgccggc gttccgcccc
      901 ggcctctacc cgaccggcga cggcgacaac tcggtgctgc tctacgatcc ggccgccgat
      961 cgggtctacc agttcaacca gttctcctac gggacgaccg aggccacggc gcgggcctgc
     1021 ttcagctacc ccctgtccgg ccgcgacgtg cgcaaccgct gggacgatcc ggggatgtgg
     1081 ggcccgggtg cgatcgactg gcggcatccc ggcggtttcc tccgcgggcg cgaggtcgac
     1141 ctcaccggcg cggtgcagat ccgccacgtc ctcaacgcca cggccacccg gcactccggc
     1201 gctggtgcta cggcggccca gcatgcgctc agcaagcgca tggcctatcc cgcatgggag
     1261 accgatcggc cgctcgacgc caacgacaac ctgggcgatc tgccctacgg cacgatcgtc
     1321 gccatccgcc ggcaggacgc cgggctgcgc gagacgctgg gcctatcgcc attcggcaag
     1381 cggctgttcg acacgcttct tgccggggag ggctacggct gctgcatcgg cgacggccag
     1441 gggcagatcg tcgacaacgc cccggtgctt cagcttcggg tggattccga gctggccgag
     1501 aacgcagcca aggtccgcgc ggtcgacgag gcgctggcca agctgctgcc gcacctgtgg
     1561 cccgtgttca ccacccgccg gcatgatgcc taggaggttc cgatggactg gcttctgatc
     1621 gctcttgttg tggcgctcct ggtgggtgcc gtcctgtgga tgctgcgcgg gccgggcgac
     1681 ggcgacgacg acaccacgcc gcagcgctgg ggcggtggtg gcggtcgcgg gcggcgttga
     1741 cagccatgca ggcgcggccg caccctccgg gccatgcgac ggtggtggct cgtcctggcg
     1801 atgctctgcg cgatcttcgc cctgatcggc tggggcgcgg gcggggcacg ccggcgccgc
     1861 aaaccatagc tacgcgcccg cggctggtcc tcgcctggct gtgcctgctc gcggcgggca
     1921 tggccatggc gctcaccggg caggtgacgt ggcacgtctg gggccacgag gtttacggcg
     1981 agtacgtcgt ccagttcctt gccctcggcc tagccggg
//
LOCUS       DAOQYE010000391         2018 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_65035_length_2018_cov_2.911360, whole genome shotgun sequence.
ACCESSION   DAOQYE010000391 DAOQYE010000000
VERSION     DAOQYE010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65035_length_2018_cov_2.911360"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..823)
                     /locus_tag="PKN52_06970"
     CDS             complement(<1..823)
                     /locus_tag="PKN52_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HNQ99725.1"
                     /translation="MRIVSIEELPRHVGERVQVNGWLTGLRSSGKIAFLQLRDGSGFV
                     QGVLAKNEVDEATFELARSLAQESAVAVTGEVRADPRSPNGVELGLASVALIGRSGDY
                     PITPKEHGVDFLFEHRHLYLRHKTPWAIMRVRDEVERGIHDFFSDRGFLRFDAPFFMP
                     TAVEGTTNLFEISLFDEGTAYLSQSGQLYGEAGALALGKIYTFGPTFRAEKSKTRRHL
                     LEFWMVEPEIAYLEHEGNMQLQEDMVAYLVGRVLDKRRPELEILGRDVSKLEPTAN"
     gene            complement(820..1644)
                     /locus_tag="PKN52_06975"
     CDS             complement(820..1644)
                     /locus_tag="PKN52_06975"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNQ99726.1"
                     /translation="MIPLVVLDLDGTIVGASGLVQECVWEAAARARDAGIKIAVCTGR
                     PGFGVAQRIAQRLGPDNPHVFQNGAYLGYPDGRTVKAVALKDDALHQIIEVSRQRGVV
                     LELYTPNSLYVERTTDLSEAHASMIGVTAIVRDLEDVVATEPVVRAQWVLPAGDYPGL
                     AEAAPAGVQLSPATSPALRGIAFVSITRTGVSKASAVKQLAEQMRIDLTDVMGVGDSH
                     NDLPMLGVVGHPRVMANAAPELLELYASVGDVEECGVVPALEEAMGVNGGLLHSHA"
     gene            complement(1641..>2018)
                     /locus_tag="PKN52_06980"
     CDS             complement(1641..>2018)
                     /locus_tag="PKN52_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit C"
                     /protein_id="HNQ99727.1"
                     /translation="DPGSVLAHAYDLVLNGSEVGGGSLRIHRLDVQERMFKALGFTRE
                     EAERRFGFFLEALAHGAPPHGGIAWGLDRLVMLLSGAPSIRDTIAFPKNQRGADPLTG
                     APAAIDERQLRELGLAVVGAVEA"
BASE COUNT          300 a          684 c          717 g          317 t
ORIGIN      
        1 cgttggcggt cggctccagc ttcgagacgt cgcggccgag aatctcgagc tctgggcggc
       61 gcttgtcgag gacgcggccg accaggtacg cgaccatgtc ctcctggagc tgcatgttgc
      121 cctcgtgctc caggtaggcg atctcgggct cgaccatcca gaactcgagg aggtgcctgc
      181 gcgtcttgct cttctccgcg cggaacgtag ggccgaacgt gtagatcttg ccgagggcca
      241 gcgcgccggc ctccccgtag agctggcccg actgcgacag gtaggcggtc ccctcgtcga
      301 agagcgagat ctcgaagagg ttggtcgtac cctccacggc cgtcggcatg aagaaaggcg
      361 cgtcgaagcg caggaagccg cggtcggaga agaagtcgtg tatgccccgc tcgacctcgt
      421 cgcgcacgcg catgatggcc cacggcgtct tgtgcctgag gtagaggtgg cggtgctcga
      481 agaggaagtc gaccccatgc tccttcgggg tgatggggta gtcgccgctg cgcccgatca
      541 gggcgacgct ggcgagcccc agctcgacgc cgttgggcga gcgcgggtcg gccctcacct
      601 cgccggtgac ggcgaccgcc gactcctgcg ccagtgaacg cgcgagctcg aacgtggcct
      661 cgtcgacctc gttcttggcc agtacgccct gcacgaagcc gctgccgtcc ctgagttgca
      721 ggaaggcgat cttgccgctc gaacggaggc ccgtgagcca gccgttcact tgcacccgct
      781 cgccgacgtg acgcggcaac tcctcgatgc tgacgatcct catgcgtgag agtgtaacaa
      841 cccgccgttc acgcccatgg cctcttccag tgccgggacc acgccgcact cctcgacgtc
      901 cccgacggag gcgtagagct cgagcagctc cggcgcggcg ttcgccatga cgcgcggatg
      961 gccgacgacc ccgagcatgg gcaggtcgtt gtggctgtcg ccgactccca tcacgtctgt
     1021 caggtcgatg cgcatctgct cggcgagctg cttgacggcg ctggccttgg agacgcccgt
     1081 cctcgtgatg gagacgaagg cgatgccgcg cagcgcgggc gaggtggcgg gcgagagttg
     1141 cacgccggca ggcgcggcct cggcgaggcc ggggtaatca ccggccggga gcacccactg
     1201 ggcgcgtacc accggctcgg tggcgacgac gtcctcgagg tcgcgcacga tggccgtcac
     1261 gccgatcatg ctggcgtgcg cctcggagag gtccgtcgtg cgctcgacgt agagggagtt
     1321 tggcgtgtag agctccagca cgacgccacg ttgccgcgag acctcgatga tctggtggag
     1381 ggcgtcgtcc ttgagcgcca cggccttcac cgtccggccg tcggggtaac cgaggtaggc
     1441 gccgttctgg aacacgtgcg ggttgtcggg cccgaggcgc tgggcgatgc gttgcgcgac
     1501 cccgaacccg ggcctgccag tgcatacggc gatcttgatc ccggcgtccc tcgccctggc
     1561 ggcggcctcc cacacgcact cctgcacgag gccggaggcg ccgacgatgg tgccgtccag
     1621 gtcgagcacg acgaggggta tcacgcctcg accgccccca cgacggccag gccgagctcg
     1681 cgtagctgac gctcgtcgat ggccgccggc gcgcccgtta gcggatcggc gccgcgctgg
     1741 ttcttcggga aggcgatcgt gtcgcggatg ctcggggcgc cggagagcag catgacgagc
     1801 cgatcgaggc cccaagcgat gccgccgtgc ggcggtgcgc cgtgcgccag ggcttccagg
     1861 aagaagccga agcgccgctc cgcctcctcc cgcgtgaagc cgagcgcctt gaacatgcgt
     1921 tcctgcacgt cgagcctatg gatgcgcaga ctgcccccgc ccacctccga gccgttgagg
     1981 acgaggtcgt aggcgtgagc gaggacgctg ccggggtc
//
LOCUS       DAOQYE010000392         2016 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_65121_length_2016_cov_2.604284, whole genome shotgun sequence.
ACCESSION   DAOQYE010000392 DAOQYE010000000
VERSION     DAOQYE010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65121_length_2016_cov_2.604284"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..514
                     /locus_tag="PKN52_06985"
     CDS             <1..514
                     /locus_tag="PKN52_06985"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HNQ99728.1"
                     /translation="SYLVGRDLVEKLKRLGIYASSFDNSYTQAISSASLGSGDVAVAI
                     TYSGETRGVIENLAMARDQGALTIALTNFRDSTIIQAADIVVPTSVTQHLLPDGSLGG
                     RIAQLLVVDLLFVRLFATDAERFRSAFTKYNRILLQKLGRPDQRFDFAQEPWRQPGGR
                     AEDDTEHDQD"
     gene            534..1583
                     /locus_tag="PKN52_06990"
     CDS             534..1583
                     /locus_tag="PKN52_06990"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HNQ99729.1"
                     /translation="MSDSMRTATFYGAEDIRLEELRRPAAEPGKVLVRVTSTGICTFE
                     RRIYSGAQKHIPMPVIAGHEVAGRVVAAPADSGFSEGDKVALDLVYRCGRCYYCVRGM
                     DNQCVRLFKEGLTWEGRHLMGGGFGEYVSVDPAKVFKVDESVPDHIAALAEPLGCVLH
                     SFGKTTVGLGDTVAVIGAGTMGSLHVALARLQGCKVLVSDPDAERRAFVEERLGATAT
                     VDPTTHDPVQAAKELTDGRGVNAVFVTAGVAAAAEQAIDMTGKYGTVVLYAASYPPAK
                     IAVDWNRIHYNEIRIDGTESRTRDDVRRAVQLLPGLDLEPLVSRRIGLDDLPGELALG
                     VPRGATQRVVVSMAS"
     gene            1733..>2016
                     /locus_tag="PKN52_06995"
     CDS             1733..>2016
                     /locus_tag="PKN52_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99730.1"
                     /translation="MQSKLAKRRRMQRIFRENGRSLIVAMDHGSFMNVLPHSADPGRI
                     LDEVREGGADAVLTTMGIASTFADRFGRMSLIMRVDGAGSNLDNSMVFEQR"
BASE COUNT          341 a          717 c          670 g          288 t
ORIGIN      
        1 gtcctacctg gtcggcaggg acctggtgga gaagctgaag cgcctcggca tctacgcgtc
       61 gagcttcgac aactcctaca cccaggccat ctcctccgcc agcctcggct cgggggacgt
      121 cgcagtggcc atcacgtact cgggcgagac ccgcggcgtt atcgagaacc tggccatggc
      181 gcgagaccag ggggccctca ccatcgccct cacgaacttc cgcgactcca ccatcatcca
      241 ggcggccgac atagtggtcc cgacgtccgt gacgcagcac ctgctgcctg acggcagcct
      301 cggcgggcgc atcgcccagc tgctcgtggt cgacctgctc ttcgtccgcc tcttcgccac
      361 cgacgccgaa cgcttccgct ccgctttcac caagtacaac cgcatcttgc tccagaagct
      421 aggcaggccg gaccagcgct tcgacttcgc tcaggagccg tggcgccagc caggtggccg
      481 agccgaggac gacaccgaac acgaccagga ctgaccccga ggagggacgt caagtgagcg
      541 attcgatgag aactgcgacc ttctacggtg ccgaggacat ccgcctcgag gagctcaggc
      601 ggccagcggc cgaaccgggc aaggtcctcg tcagggtgac gagcacgggc atctgcacgt
      661 tcgagcgccg gatctacagc ggggcccaga agcacatccc catgcccgtg atcgcgggtc
      721 acgaggtcgc gggccgcgtc gtggcggcgc cggccgacag cggcttctcc gagggcgaca
      781 aggtggcgct cgacctcgtc taccggtgcg gccgctgcta ctactgcgtg cgcggcatgg
      841 acaaccagtg cgtgaggctc ttcaaggagg gcctgacctg ggaaggcagg cacttgatgg
      901 gcggaggctt cggcgagtac gtgtccgtcg accccgcgaa ggtcttcaag gtggacgagt
      961 cggtgccaga ccacatcgcc gcgctcgccg agcccctcgg ctgcgtgctg cacagcttcg
     1021 gcaagacgac cgtcgggctc ggcgacaccg tcgccgtcat aggcgccggc accatgggct
     1081 cgctgcacgt cgccctggcc aggctgcagg ggtgcaaggt gctcgtcagc gaccccgacg
     1141 ccgagcgtcg cgcgttcgtc gaggagcgcc tcggcgccac ggcgaccgtc gacccgacca
     1201 cccacgatcc ggtccaggcc gccaaggagt tgacggacgg ccgcggcgtc aacgcggtct
     1261 tcgtcaccgc cggcgtggcc gccgccgcgg agcaggcgat agacatgacc ggcaagtacg
     1321 gcacggtcgt gctgtacgcc gcgagctacc cgcccgccaa gatcgccgtc gactggaacc
     1381 gcatccacta caacgagatc cgcatcgacg gcaccgagtc gcgcacgcgc gacgacgtgc
     1441 gcagggcggt gcagctcctg cccgggctcg atctggagcc gctcgtctcg cgccggatag
     1501 gccttgacga cctccctggc gagctcgcac tcggcgtgcc gagaggcgcc acgcagcgcg
     1561 tcgtcgtgag catggcttct tgaggaggcc ccggcgtcgc gatcgcggag taacgagccc
     1621 gtgagcacgg cgtcctgagc acggcgtaac gagcccaacc catggcggcc ctgccgggac
     1681 cgccttcgag gcccactagc ccgcgggcgt ctcgcccgca ccggaggaca cagtgcagag
     1741 caaactggcc aagagacgta ggatgcagcg gatcttccgc gagaacggcc gttccctgat
     1801 cgtggcgatg gaccacgggt cgttcatgaa cgtgctcccc cattcggccg acccgggacg
     1861 gatcctcgac gaggtgcgcg agggcggcgc cgacgcggtc ctcaccacca tgggcatagc
     1921 gtcgacgttc gccgaccgct tcggacgcat gagcctcatc atgcgcgtgg acggcgcggg
     1981 ctcgaacctc gacaactcca tggtcttcga gcagcg
//
LOCUS       DAOQYE010000393         2010 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_65447_length_2010_cov_2.883887, whole genome shotgun sequence.
ACCESSION   DAOQYE010000393 DAOQYE010000000
VERSION     DAOQYE010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65447_length_2010_cov_2.883887"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1238)
                     /locus_tag="PKN52_07000"
     CDS             complement(<1..1238)
                     /locus_tag="PKN52_07000"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HNQ99731.1"
                     /translation="MTPVFTILVPLVTAALVLLLRNSTPLMRVVALAGAVATLVLAVL
                     LPGSRPFSAEWLPGFGVSFSVDGNGAASVLVFAVALMMIPALLVALTRVEKGAAGFAA
                     LLLAAEAGLAGIFMARDLIVFYVFWEATLVPGLLLLGLYGGEKRREATTKYLVYAVTG
                     SFFMLVSILALKTLSGAASFDVIDLMLVTPDLSVATQTWLFVGLALGMAVKLPIWPLH
                     SWLVDLNEQNHPSGAADVLGSLYKVGAFGFFAWGIPLLPAGASRVAPILIALAAVTAL
                     YGAFGAIGSKHLKRFLAYGSLSHMGIIGVGIFGMHLAGLNGAMYFLAAQMVSTGGLFL
                     VGGMLYHRRRSFDLADYGGIAKSAPALAALGLLTIFAFIGVPGLSNFPGEFMSLMGAF
                     QNAPWPAAIATLAVIAAGVYG"
     gene            complement(1235..>2010)
                     /locus_tag="PKN52_07005"
     CDS             complement(1235..>2010)
                     /locus_tag="PKN52_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HNQ99732.1"
                     /translation="ATLAIAGVPFLSGFFSKDAIVVATFTSGVLAGVGGEVLYALLMV
                     TAGITAFYMFRWYYLVFAGEERLERGVSEKVHESPGVMTVPLTILAIGSVLVGYLGLP
                     SFLADNRIAAWLGHATAAGEFSHPSVATEWLLLIASVVVAFIGLGLGYWVYAVGKGAL
                     AKRLGNAPIAGASRGAFGFDTLYRALFVDTGAGTAEAVTLLDREVVDRGLVGLSGVVP
                     LIGGLLRRWQSGQVRAYAFAMLVGAACLVAAAAIVGVLK"
BASE COUNT          376 a          659 c          754 g          221 t
ORIGIN      
        1 ccgtacacgc ccgccgctat gaccgcgagg gtggcgatgg ccgccggcca cggcgcgttc
       61 tggaaggccc ccatgagcga catgaactcg ccggggaagt tcgagagccc tggcacgccg
      121 atgaacgcga agatggtgag gagcccgagg gctgcgagcg ccggcgcgga cttggcgatg
      181 ccgccgtagt cggcgaggtc gaacgaccgc cgacggtggt agagcatgcc gccgacgagg
      241 aagagcccgc cggtcgagac catctgcgcg gccaggaagt acatggcgcc gttgaggccc
      301 gccaggtgca tgccgaagat gccgaccccg atgatgccca tgtgggagag ggagccgtag
      361 gcgaggaagc gcttgaggtg cttcgagccg atggcgccga acgcgccgta gagcgccgtg
      421 acggcggcca gggcgatgag gatgggcgcg acgcgcgacg ctccggccgg caggagcggg
      481 atgccccacg cgaagaagcc gaacgccccg accttgtaga gcgagccgag cacgtcggcg
      541 gcgccggaag ggtggttctg ctcgttcagg tcgacgagcc aggagtgcag cggccagatg
      601 ggcagcttga cggccatgcc gagcgccagc ccgacgaaca gccacgtctg ggtggctacg
      661 ctcaggtcgg gggtgacgag catgaggtcg atgacgtcga acgacgcggc gccggagagc
      721 gtcttcaggg ccaggatgct gacgagcatg aagaacgagc cggtgacggc gtacacgagg
      781 tacttggtcg tcgcctcgcg gcgcttctcg ccgccgtaga gcccgaggag gaggagcccg
      841 gggacgagcg tggcctccca gaagacgtag aagacgatga ggtcgcgcgc catgaagatg
      901 cccgccagcc ccgcctcggc cgccagtagc agcgcggcga aaccggcggc gcccttctcg
      961 acgcgtgtga gggcgacgag cagggccggg atcatcatca gcgcaacggc gaagacgagc
     1021 accgaggccg cgccgttgcc gtcgacgctg aagctcacgc cgaaccctgg cagccactcg
     1081 gccgagaagg gcctggagcc gggcaggagc acggccagca cgagggtggc gacggcgcct
     1141 gccagcgcga cgacccgcat gaggggcgtc gagttcctca gcaggagcac gagcgcggcc
     1201 gtgacaaggg ggacgaggat ggtgaagacg ggcgtcactt gagcaccccc acgatggcgg
     1261 ccgccgctac caggcaggcc gctccgacga gcatcgcgaa cgcgtaggcc cgcacctgcc
     1321 cggactgcca gcgccgcaac aggccgccga tgagcggcac gacgccgctc aggcccacca
     1381 ggcctcggtc gacgacctcg cggtcgagca gcgtgaccgc ctccgcggtg ccggcgccag
     1441 tgtcgacgaa cagggcccgg tagagggtgt cgaagccgaa ggccccccga gaagccccgg
     1501 cgatcggcgc gttgccgagc cgcttggcca aggccccctt gccgaccgcg tagacccagt
     1561 acccgaggcc gaggccgatg aaggccacga ccacggaggc tatgagaagc agccactcgg
     1621 tcgctacgct cgggtgcgag aactcgcccg cggccgttgc gtggccgagc cacgccgcga
     1681 tgcggttgtc cgcgaggaag gagggtaggc cgaggtagcc gacgaggacg ctgccgatgg
     1741 cgaggatggt cagcggcacg gtcatgacgc cgggcgactc gtggaccttc tcgctcacgc
     1801 cgcgctcgag ccgctcctca cccgcgaaga cgaggtagta ccaccggaac atgtagaacg
     1861 ccgtgatgcc ggccgtgacc atcaggaggg cgtagagcac ctcgccgccc accccggcga
     1921 gcacgccgct cgtgaaggtg gcgaccacga tggcgtcctt gctgaagaag cccgacagga
     1981 acggcacgcc cgcgatggcg agggtagcga
//
LOCUS       DAOQYE010000394         2008 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_65565_length_2008_cov_2.646697, whole genome shotgun sequence.
ACCESSION   DAOQYE010000394 DAOQYE010000000
VERSION     DAOQYE010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65565_length_2008_cov_2.646697"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..490)
                     /locus_tag="PKN52_07010"
     CDS             complement(<1..490)
                     /locus_tag="PKN52_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HNQ99733.1"
                     /translation="MSLRTVKEHLRRYGKADAVLEFAESSATVALAAEKVGTEPARIA
                     KTLAFYDPEDQGRALLVVAAGDARLHNGSFKRRFGGKPKMLAADDVEALTGHPVGGVC
                     PFANPPDVRVYLDESLRRFEVVYPAAGTPASAVPFTPVELEQASGALGWVEVTNGWRG
                     EDT"
     gene            550..1182
                     /gene="trmH"
                     /locus_tag="PKN52_07015"
     CDS             550..1182
                     /gene="trmH"
                     /locus_tag="PKN52_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0009020 - tRNA
                     (guanosine-2'-O-)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HNQ99734.1"
                     /translation="MTLRRRERIHDVLAKRQRDLTVLAEEVHKPHNLSAILRSCDAVG
                     IGTVHAVKPTGGVATFSATSASADKWVELVVHPGVDAAIAAVKAAGMRVYAAHLSSEA
                     VDYRAVDYTVPCAVLLGNERDGVSPGAAALADQHVIIPMLGMVQSLNVSVAAAVILFE
                     AQRQRLAAGKYDHPSLSAEELRELALRWLPRWATSGEVDPPLTGLTEDID"
     gene            1192..>2008
                     /locus_tag="PKN52_07020"
     CDS             1192..>2008
                     /locus_tag="PKN52_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HNQ99735.1"
                     /translation="MTDKPQPPKYRRGPGGYVGAFTALAFTLALAACTQNVTAQRLVP
                     VADGIRQPVVITNAGDERLFVAEQSGAIRVISSGELLRDPFLDLSGELSTGGERGLLG
                     LAFPSDYQETGRFYVYYTGDGGQSVLSRFSVTPGNPNRANPDSEEVLLTQEQPFSNHN
                     GGQIVFGPDGYLYVGLGDGGSGGDPQGNGQNLGTFLGKLLRLDVSGDAGYAVPPSNPF
                     VDRDGARDEIWAYGLRNPWRFSFDRETGDLYIADVGQDAYEEVNFVAAGTGAGL"
BASE COUNT          329 a          724 c          641 g          314 t
ORIGIN      
        1 ccgtgtcctc accgcgccaa ccgttcgtga cctccaccca gccgagcgct cctgaagcct
       61 gctccagttc gaccggcgtg aacgggaccg ccgacgccgg cgtgccggcg gccgggtaga
      121 ccacttcgaa gcgccggagc gactcgtcca ggtagacccg cacatcgggt gggttggcga
      181 acgggcagac gccacccacc gggtgaccgg tcagcgcctc cacgtcgtcg gccgccagca
      241 tcttcggttt cccgccgaaa cggcgtttga acgagccgtt gtgcaacctg gcgtcgcctg
      301 ctgcgaccac gagtagcgcc cgcccttggt cctctgggtc gtagaacgcc agggtcttcg
      361 cgatccgcgc aggctccgtc ccgaccttct cggcggccaa cgccacggtg gcgctggact
      421 ccgcgaactc caggaccgca tccgccttcc cgtaacgcct taggtgctcc ttcaccgttc
      481 gcaaggacat ccgtgtacct taaccgctcc cgcgcctcgg agcaccgacc ggccggttat
      541 cctccccgta tgaccttgcg gcgccgtgag cgcatccacg acgttctcgc gaaacgccag
      601 agagacctga ccgtgctcgc cgaggaggtg cacaagccgc acaacctctc cgccatcctg
      661 agaagttgcg acgccgtcgg catcggcacg gtgcacgccg tcaagccgac ggggggcgtg
      721 gccaccttca gcgcgacgag cgccagcgcc gacaagtggg tggaactggt cgtgcacccc
      781 ggcgtagacg ctgcgatagc cgccgtcaag gcggcgggca tgcgggtata tgccgcgcac
      841 ctctcatccg aggccgtcga ttaccgggcc gtggactaca ccgtgccgtg cgccgttctc
      901 ctaggcaacg agagggatgg cgtcagccca ggcgcggccg cgctcgccga ccagcacgtc
      961 atcatcccga tgctcggcat ggtgcagtcg ctcaacgtca gcgtggccgc cgccgtgatc
     1021 ctgttcgagg cccaacgtca gcgcttggcc gccggcaagt acgaccaccc cagcctgagc
     1081 gccgaggagc tgcgggaact cgcgctcagg tggttgccgc ggtgggccac ctcaggagag
     1141 gttgatcctc cgctcaccgg actcacggag gatatcgact aggctctagg catgaccgac
     1201 aagcctcaac ccccgaagta ccggcgcgga ccaggcggtt acgtcggcgc gttcaccgcc
     1261 ctagccttca cgttggccct ggccgcttgc acccagaacg tgaccgctca acggctcgtg
     1321 cccgtggcgg acgggatcag gcagcccgtc gtcatcacca acgccgggga cgagcgcctg
     1381 ttcgtggcag agcagagcgg cgccatccga gtgatcagca gcggcgagct gctccgcgac
     1441 ccgttcctcg acctttccgg cgagctcagc acggggggcg agcgcggcct gctcggcctc
     1501 gctttcccgt ccgattacca ggagaccgga cgcttctacg tttactacac tggcgacggc
     1561 ggccagagcg tcctctctcg tttcagcgtc acgccgggca accccaaccg cgccaacccc
     1621 gattcggaag aggtcctcct cacgcaggag cagccgttct ccaaccacaa cggcggccag
     1681 atcgtgttcg gaccggacgg ctacctgtac gtggggctcg gcgacggtgg cagcggtggc
     1741 gacccgcaag gcaacggaca gaacctcggt acgttcctcg gcaagctgct gcgcctggac
     1801 gtgtcgggcg acgccggcta cgccgtcccg cccagcaacc ccttcgtcga tcgtgacggc
     1861 gcgcgcgacg agatctgggc gtacggactg cgcaacccgt ggcgtttctc tttcgatagg
     1921 gagacgggcg acctctacat cgcggacgtt ggccaagacg cttatgagga agtcaacttc
     1981 gtggcggcgg gcacgggcgc cgggctca
//
LOCUS       DAOQYE010000395         1995 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_66232_length_1995_cov_3.122680, whole genome shotgun sequence.
ACCESSION   DAOQYE010000395 DAOQYE010000000
VERSION     DAOQYE010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66232_length_1995_cov_3.122680"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..987)
                     /gene="gap"
                     /locus_tag="PKN52_07025"
     CDS             complement(<1..987)
                     /gene="gap"
                     /locus_tag="PKN52_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HNQ99736.1"
                     /translation="MKIGINGFGRIGRQIFRIAHQRGLEVVLVNDLTDTTTLAHLLKY
                     DSNYGRFPGDVSHDDKNIIVDGSKVAVTAHKDPAEIPWGEHGVDIVVESTGIFTKRAQ
                     AAVHLAGGAKKVLISAPSPDPDFDIMLGVNQDEYDPKKHDVVSNASCTTNSLAPVMKV
                     LDDTFGIEQALMTTIHSYTNDQRILDAPHKDLRRARNAASNIIPTSTGAAQAVGKVLP
                     QLDGIFNGSSLRVPTQTGSISDITALLKREVAASDINAALEAAANGPLKGIVKYTTDP
                     IVLADIVGDPHSGIVDSLLTKVQGRLAKVFVWYDNEWGYSNRVVDVITIMGKT"
     gene            complement(1159..>1426)
                     /locus_tag="PKN52_07030"
     CDS             complement(1159..>1426)
                     /locus_tag="PKN52_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5915 domain-containing protein"
                     /protein_id="HNQ99737.1"
                     /translation="RKAAGLDVADRIVLGVAAGGELAEAVREHGATLAREVLARDVLA
                     SGADAAVADATLADTALAGVALAGAYVERHEVDGLPLTIALKKA"
     assembly_gap    1427..1436
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1437..>1995)
                     /locus_tag="PKN52_07035"
     CDS             complement(<1437..>1995)
                     /locus_tag="PKN52_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5915 domain-containing protein"
                     /protein_id="HNQ99738.1"
                     /translation="LMRLVELGRAARAASGVKLRQPLPEVLVRVRSEEELAGVKNLSD
                     QLLDELNVKAVRFLAVTDDFVDYAIKPNLPRLGKRVGKLIPQLRVALERLDGRAVAAA
                     VRAGSAVSVTLADTTLELEPEDLLLDARSPEGYAAQEERGYLVALDTRVSPELRREGL
                     ARDVVRLVQNARKAANREGCNLLILD"
BASE COUNT          260 a          652 c          717 g          356 t
ORIGIN      
        1 ggtcttgccc atgatggtga tgacgtcgac cacgcggttg gagtagcccc actcgttgtc
       61 gtaccagacg aacaccttgg cgaggcgccc ctggaccttg gtgaggaggc tgtcgacgat
      121 ccctgagtgc gggtcgccga cgatgtcggc cagcacgatc gggtccgtcg tgtacttgac
      181 gatgcctttc agcgggccgt tggctgcggc ttcgagggcg gcgttgatgt cgctcgccgc
      241 gacctcgcgc ttgaggaggg ccgtgatgtc cgagatggag cccgtctggg tcggcacgcg
      301 cagcgaggag ccgttgaaga tcccgtcgag ctgcggcagc accttgccaa cggcctgggc
      361 cgcgcccgtg gaggtcggga tgatgttcga ggccgcgttg cgcgcgcgtc gcaggtcctt
      421 gtgcggggcg tcgaggatgc gttggtcgtt cgtgtaggag tggatggtgg tcatgagcgc
      481 ctgctcgatg ccgaacgtgt cgtcgagcac cttcatgacg ggtgcgagcg agttcgtcgt
      541 gcaagaggcg ttggagacca cgtcgtgctt cttggggtcg tactcgtcct ggttgacgcc
      601 cagcatgatg tcgaagtccg ggtccgggct aggtgcgctg atcaggacct tcttggcgcc
      661 gcctgccagg tggacggcag cctgggcgcg cttggtgaag atgcccgtcg actcgaccac
      721 gatgtcgacg ccgtgctcgc cccacgggat ctccgccgga tccttgtgcg ccgtgacggc
      781 gaccttgctg ccgtcgacga tgatgttctt gtcgtcgtgg ctcacgtcgc cggggaagcg
      841 cccgtagttc gagtcgtact tgagcaggtg cgcgagggtc gtcgtatccg tcaggtcgtt
      901 gacgaggacg acttccagac cgcgttgatg cgcgatgcgg aagatctgcc ggccgatgcg
      961 gccgaaaccg ttgatgccta tcttcatgac tgtcgcctcc cagtggcggg tgagtaggag
     1021 ctgtgtcgac gccctcgtta ccggggcgcg tcctggacca cccgattcta tcgcggccgc
     1081 gtgaggcgtt tagtccctgt cgctatagcc gctgtctttc ggaggaccgc ccgccgcggt
     1141 cctccgaaag gcggcagctc aggccttctt gagggcgatg gtgagcggca gaccgtccac
     1201 ctcgtggcgc tcgacgtatg cgcctgccaa ggcgacgcct gccaaggcgg tgtccgctaa
     1261 ggtggcgtcc gccaccgcgg cgtccgcgcc gctggcgagg acgtcgcggg ccaggacctc
     1321 cctggccagc gtcgccccgt gctcgcgcac ggcctcggcc agctcgcccc ctgccgccac
     1381 gccgagcacg atgcggtcgg cgacgtcgag cccggccgcc ttgcggnnnn nnnnnngtcg
     1441 aggatcagga ggttgcagcc ctcgcggttg gccgccttgc gggcgttctg cacgagccgg
     1501 acgacgtcac gcgccaggcc ctcccggcga agctccgggc tgacgcgcgt gtcgagggcc
     1561 acgaggtagc cgcgctcttc ctgcgccgcg tacccctccg ggctcctggc gtcgaggagc
     1621 aggtcctccg gctcgagctc gagcgtggtg tcggccagcg tcacgctcac cgcgctgccg
     1681 gcgcgcacgg cggcggccac ggcccgaccg tccagccgct cgagcgcgac ccgcagctgc
     1741 gggatgagct tgccgacgcg cttgccgagg cgcgggaggt tcggcttgat ggcgtaatcg
     1801 acgaagtcgt ccgtgacggc gaggaagcgc acggccttga cgttcagctc gtccagtagc
     1861 tgatcgctga ggttcttgac gccggccagc tcctcctcgc tgcgcacgcg cacgaggacc
     1921 tcgggcaggg gctggcggag cttgacgccc gaggccgccc gcgccgcccg gcccagctcg
     1981 accaggcgca tgagc
//
LOCUS       DAOQYE010000396         1989 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_66590_length_1989_cov_3.135471, whole genome shotgun sequence.
ACCESSION   DAOQYE010000396 DAOQYE010000000
VERSION     DAOQYE010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1989
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66590_length_1989_cov_3.135471"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..349)
                     /locus_tag="PKN52_07040"
     CDS             complement(5..349)
                     /locus_tag="PKN52_07040"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HNQ99739.1"
                     /translation="MVVHRGEVWWADLGQPRGSAPGFRRPVLIVQSDAFNQSRIRTVL
                     CVMLTSNLALLAAPGNVLVENGRSGLPKDSVANVSQVVTLDRDHLVERVGQVGFDLLA
                     EIDAGLRLVLDL"
     gene            complement(623..1240)
                     /locus_tag="PKN52_07045"
     CDS             complement(623..1240)
                     /locus_tag="PKN52_07045"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HNQ99740.1"
                     /translation="MFDLTTFWVFLTAAVTLAIIPGPGMFYVLTRTLRGGRREGLLST
                     VGTGIAGLVHTFAAALGVSAILATSALAFAVVKWLGAAYLVYLGIKTLLGGSGFSAAD
                     VAQAPSGGALRQGIIAEILNPKTALFFLAFIPQFVHPGGGVFWQFVLLGSITTLLTSG
                     VDLLVVLGASPLSVLMRRNRAVRIAQRYGTGTALVGLGVYVAVER"
     gene            1336..>1989
                     /locus_tag="PKN52_07050"
     CDS             1336..>1989
                     /locus_tag="PKN52_07050"
                     /inference="COORDINATES: protein motif:HMM:NF016207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HNQ99741.1"
                     /translation="MYVPPHNREERPEVMHQLIRTRPLGLLISVGNNGPVANPIPFVL
                     HAEGASLGTLECHLARANPQWRDLQTATDCLIAFQGPQAYVTPSWYPAKREHGKVVPT
                     WNYVIVQVRGRPTIIEDHVWLRRHLEELVDQNEEPFDEPWRMTDAPEEYLAAQMKGIV
                     GLSIEVTELVGKWKLSQNRSVADQQGTALGLLARGGSHADVGDMMADMLEVKGCPVRH
                     "
BASE COUNT          397 a          693 c          604 g          295 t
ORIGIN      
        1 cgtcttacag gtccagcacg agcctcagac ccgcgtcgat ctcggcaagg aggtcgaacc
       61 cgacctggcc gacacgctcg accaagtgat cgcggtccag tgtcactact tgcgacacgt
      121 tcgccaccga gtcctttgga agaccagatc gaccgttctc aaccaacacg ttgccgggag
      181 cagcgagcaa ggcaaggttc gaggtaagca tcacgcatag tacggtgcga atccgactct
      241 ggttgaacgc atcggactga actatcagga cggggcgtcg aaacccagga gccgagcccc
      301 ggggttggcc caggtctgcc caccagacct cgccgcgatg aactaccaat cgtccgtacc
      361 cacactgacg gcttgtgcat cacgtaggcc ggcttccaac ccgctatcct ctcgcgagta
      421 aagctggttg agcttcgcag tcacctcctg gtgccgatgc ttcgccaagt actctctgac
      481 cgccctggag tacaactcgc ttcgagacaa ccccagcatg tcagccaacc ggtcggcctc
      541 ccggtagacg tcctccggca cggagatggc agttttcata ccttcatcct accgtggtta
      601 taccgcggcc gtccgaaccg cctcacctct ccacggccac atagacccca agcccaacga
      661 gcgccgtccc cgtcccgtac cgctgagcga tccgcacggc ccggttccgc ctcatgagca
      721 cgctcaacgg gctggcgcca agcaccacga gcaggtccac cccgctggtg agcagagtcg
      781 tgatggaccc aagcagcacg aactgccaga acaccccgcc tccagggtgc acgaactgcg
      841 ggatgaacgc gaggaagaag agcgccgtct tggggttcaa gatctcggcg atgatgccct
      901 gcctgagcgc cccaccggag ggcgcctgag cgacatcggc ggccgagaac cccgacccgc
      961 cgagcaaggt cttgatccct aggtagacga ggtaggccgc gcccaaccac ttcacgacgg
     1021 cgaacgcgag ggccgacgtc gcgagtatcg cggagacccc gagcgcggcc gcgaacgtat
     1081 gcacgaggcc ggcgatgccg gtcccgactg tcgagagcaa cccttcgcgc cggccacctc
     1141 gcagtgtgcg ggtgagcacg tagaacatcc ccggtccggg gatgatcgcc agcgtgaccg
     1201 ctgctgttag gaacacccag aacgtcgtca ggtcgaacat gcgcgcctcc gttcgcggcg
     1261 ccacgctacc acccgactcg taaggccgcg aggcgcgcca acggatctcc ggtacgtagg
     1321 tcgtacactc ctcacatgta cgtgccgccg cataaccgcg aagagcgccc cgaggtcatg
     1381 catcagctca tccgcacgcg gccgctcggg ctgctcatca gcgtcggcaa caacggaccc
     1441 gtggccaacc cgatcccgtt cgtcctgcac gcggaaggcg cctcgctcgg cacgctcgag
     1501 tgccacctcg cgcgcgccaa cccgcagtgg cgcgacctgc agaccgccac cgactgcctc
     1561 atcgccttcc agggcccaca ggcgtacgtc acgccgtcgt ggtacccagc caagcgcgag
     1621 cacggcaaag tggtgccgac gtggaactac gtgatcgtgc aggtgcgcgg gcgtccgacc
     1681 atcatcgagg accacgtctg gctgcggcgg cacttggaag agctcgtcga ccagaacgag
     1741 gagccgttcg acgagccgtg gcgcatgacc gacgcacccg aggagtacct cgccgcccag
     1801 atgaagggca tcgtgggctt gagcatcgag gtgaccgagt tggtcggtaa gtggaagctg
     1861 agccagaacc gcagcgtggc cgaccagcag ggcactgcgc tgggactgct agcacgcggc
     1921 ggcagccacg ccgacgtcgg cgacatgatg gctgacatgc tggaagttaa aggctgtccg
     1981 gtgcgacac
//
LOCUS       DAOQYE010000397         1989 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_66595_length_1989_cov_3.039297, whole genome shotgun sequence.
ACCESSION   DAOQYE010000397 DAOQYE010000000
VERSION     DAOQYE010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1989
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66595_length_1989_cov_3.039297"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="PKN52_07055"
     CDS             <1..412
                     /locus_tag="PKN52_07055"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNQ99742.1"
                     /translation="GPRWLREVEARTVGWPAYHPRPYLDATAAWSAADLAITRAGVGT
                     LSEAAFHGVPLIMIPLPTAAEDHQSHNARAVAAAGAGLVVKESKAATALPDAWAKALD
                     PAWRRSAAAAAAVRTPAGAARRIVDAVLETGRER"
     gene            409..1857
                     /gene="murC"
                     /locus_tag="PKN52_07060"
     CDS             409..1857
                     /gene="murC"
                     /locus_tag="PKN52_07060"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HNQ99743.1"
                     /translation="MTDLDTGTARGGAGQRIHFMGIGGVSMAALARWCFEEGFEVSGC
                     DASGSPVLGELAALGIRTCAGHDASHAEDVDVVVHSMAVPYDHPELAAARAHGRKVLR
                     RIALLGELFTRRSAVAVTGSHGKSTTTAMVGTLLLALDRETSIQLGASLPSIGGSMRY
                     GRGEWLAAEVDESDPGFADLVSKVAVITNLDDDHIAGEYDERRNYHASFEDLQAAAQR
                     FTLAAERLVYCADWPGLRDLVGGHPRAVNYGTGALCDYRIGPVELTSGGSRFEVTSPD
                     LGTFQVELSVPGMHNVLNATAALAATHVAGFDPRLALGALKDFTGVGRRLQSYGALNG
                     ASIVDDYAVHPTEVAVTLQVARATGLRVRAVLQPHRWVRTARQWPALAKAAALADEVV
                     VLGVYAAGERPIEGVSPELIAERVRSLGVKARVTDLAGAEEYLAATAAPGEMLVMLGA
                     GDVWRVAAGLAARAKAAGSSETSPAGAANPWR"
BASE COUNT          274 a          746 c          713 g          256 t
ORIGIN      
        1 cggaccgcgc tggctgagag aggtcgaggc gcgcacggtc ggctggccgg cctatcaccc
       61 gaggccctac ctcgacgcca ccgccgcctg gtcggccgcc gatctggcca tcacgagggc
      121 gggcgtcggc acgctctccg aggccgcctt ccacggcgtc ccgctgatca tgatcccgtt
      181 gccgacggcg gcggaggacc accagtcgca caacgcccgc gcggtcgccg ccgccggcgc
      241 cggcctggtc gtcaaggaga gcaaggccgc caccgctctg cctgacgcct gggccaaggc
      301 gctcgacccc gcctggcggc gttcggccgc cgcggccgcg gccgtcagga ccccggccgg
      361 cgccgctcgt cgtatcgttg acgccgtttt ggagaccgga agagagagat gacagaccta
      421 gacacaggaa cggcacgcgg cggcgccggc cagcgcatcc acttcatggg cataggcggc
      481 gtgagcatgg ccgccctcgc acgctggtgc ttcgaggagg gcttcgaggt aagcggctgc
      541 gacgcctccg gcagccccgt gctcggcgag ctcgcggcgc tcgggatcag gacctgcgcc
      601 ggccatgacg cttcgcacgc cgaggacgtc gacgtcgtgg tgcacagcat ggccgtgccg
      661 tacgaccatc cggagctcgc ggcagcccgc gcccatggcc gcaaggtact gcgccgcatc
      721 gcgctcctcg gcgagctgtt cactcgccgg agcgctgtgg ccgtcacggg ctcacacggc
      781 aagagcacga caacggccat ggtcgggacg ctcctcctcg cgctcgaccg cgagacctcc
      841 atccaactcg gcgcgtccct tccgtccatc ggcgggagca tgcgctacgg gaggggcgag
      901 tggctcgccg ccgaggtcga cgagtccgac ccgggcttcg ccgacctggt cagcaaggtc
      961 gcggtcatca cgaacctcga cgacgaccac atcgctggcg agtacgacga gcgccgcaac
     1021 taccacgcct cgttcgagga cctgcaagcg gcggcgcagc gcttcacgct ggcggccgag
     1081 cgactcgtgt actgtgccga ctggcccggc ctacgcgacc tggtcggcgg gcacccgcgg
     1141 gccgtcaact acggcacggg cgcgctctgc gactaccgca tcggacccgt cgagctcacg
     1201 tccggcggca gccgcttcga ggtgacctcc ccggacctcg gcaccttcca ggtggagctg
     1261 tcggtgcccg gcatgcacaa cgtcctcaac gccacggcgg cgctggccgc cacccacgtg
     1321 gcgggcttcg acccgcgcct cgcccttggg gccctcaagg acttcaccgg cgtcgggcgc
     1381 cgcctccagt cctacggcgc gctcaacggc gccagcatcg tggacgatta cgccgtccat
     1441 ccgaccgaag tggccgtcac gcttcaagtc gcacgcgcca cgggcctgcg cgtgcgcgcc
     1501 gtcctgcagc cgcaccggtg ggtgcgcacg gcgcgccagt ggccggccct tgccaaggcc
     1561 gccgccctcg cggacgaggt ggtcgtgctc ggcgtctatg ccgccggcga gcggcccatc
     1621 gagggcgtgt cgcccgagtt gatcgcggag cgcgtcaggt cgctcggcgt caaggcgcgc
     1681 gtcacggacc tggccggagc cgaggagtac ctggcggcga cggccgcgcc cggagagatg
     1741 ctcgtgatgc ttggcgcggg cgacgtctgg cgggtggccg ccgggctcgc tgcgcgcgcc
     1801 aaggccgccg gttcctccga gacgagcccc gccggcgcgg cgaacccatg gcgctgacgc
     1861 ccgccaccgc cggcccgcgc gtgaggcggg tgcgcctgcg cgactacacg accctgcgcg
     1921 tgggcgggga ggcggagctg tgggaggtcg agaacgagga ggacctgcgg gaagccacgc
     1981 gcggaccgt
//
LOCUS       DAOQYE010000398         1986 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_66775_length_1986_cov_2.541688, whole genome shotgun sequence.
ACCESSION   DAOQYE010000398 DAOQYE010000000
VERSION     DAOQYE010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1986
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66775_length_1986_cov_2.541688"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..842
                     /locus_tag="PKN52_07065"
     CDS             <1..842
                     /locus_tag="PKN52_07065"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ99744.1"
                     /translation="ARTRRRKVPPLIVACWVILGLLVTLAVFAPLIAPYDLRTQSLLQ
                     RLKPPAFMGGSADHLLGTDQLGRDMLTRLLYGLRTTLGIAAIGTVIGMTLGVTLGVLS
                     GMFGGWLDDAVMFLVDVQMSLPFVLVALTVIAMLDTSLLVLVLVVGIAGWEIHARVVR
                     GQVMAVKGLPFVEATQALGARLQRVALKHVLPNVVSPIIVLTTVNFSYVVLLESALSF
                     LGLGVQPPNTSLGALLGAGRDYMVSNWTLPALPGLLIVMITMVVSLIGDWLRDVLDPR
                     QRD"
     gene            857..>1986
                     /locus_tag="PKN52_07070"
     CDS             857..>1986
                     /locus_tag="PKN52_07070"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HNQ99745.1"
                     /translation="MERPTIAEIAERLRSKAMSPVELFDAHARRLSALEPVLNAFVTT
                     TLDEGRAAAVAAEAALARGTARGPLHGVPVAHKDIYFTRGVRTTAGSRALDDHVPAYD
                     ATVVELLAAAGAVSLGKANCHELACGAMQGFGVTRNPWDPGRAAGGSSSGSAAAVAGR
                     VVMAATASDTGGSVRVPASFTGTVGLKPTFGRVSRHGLFPISYTLDHPALLTNCVADA
                     ATVLGAMSAPDSKDRTTLARHDEARWPAFADDLAGLRLGVPAALLASGLDPEVEAALA
                     AAARRLEELGATLVPVEVAGFDLVAPTHSVIWLAEGASRLRGLIARRRASVGAVAARR
                     MLPGLLLTANDYFLAMHVRERLKESMQKLFATVDVLLLPTTP"
BASE COUNT          255 a          737 c          681 g          313 t
ORIGIN      
        1 ccgcccgaac ccggcggcgg aaggtgccgc cgctcatcgt cgcctgctgg gtcatcctcg
       61 ggctgctcgt caccctcgcc gtcttcgcgc cgctcatcgc cccctacgac ctccgcactc
      121 agagcctcct gcaacgcctc aagccgcccg cgttcatggg cggttcggcg gaccacctcc
      181 tgggcacgga ccagctcggc cgcgacatgc tcacgcggct gctctacggc ctcaggacca
      241 cgctcggcat cgcggccatc ggcaccgtca taggcatgac cctcggcgtc accctcgggg
      301 tgctgtcggg catgttcggc ggctggctcg acgacgccgt catgttcctc gtcgacgtgc
      361 agatgtcgtt gccgttcgtg ctggtcgcgt tgaccgtcat cgccatgctc gacacgagcc
      421 tcctcgtgct cgtgctcgtg gtgggcatag ccggctggga gatccacgcc cgggtggtca
      481 ggggtcaagt catggccgtc aaggggctcc cgttcgtgga ggccacgcag gcgctcgggg
      541 cccggctgca gcgcgtcgcg ctcaagcacg tgctgcccaa cgtcgtgtcc ccgatcatcg
      601 tgctcaccac cgtcaacttc agctacgtcg tgctgctcga gtccgcgctc tcgttccttg
      661 ggctgggggt gcagccgccg aacacgtcgc tcggcgccct gctcggcgcg ggccgcgact
      721 acatggtgtc gaactggacc ctgcccgcgt tgcccgggtt gctgatcgtc atgatcacca
      781 tggtcgtatc gctcatcggc gactggctgc gcgacgtact cgacccgcgc cagcgcgact
      841 aggaggcgcg ggcaacatgg aacgaccgac catcgccgag atagccgaac gcctgcggag
      901 caaggccatg tcgcccgtcg agctcttcga cgctcatgcg cgccgcctat ccgcgctcga
      961 gccggtgctc aacgccttcg tcacgaccac cctcgacgag gggcgcgccg ccgccgtggc
     1021 ggccgaggcg gccctcgcgc gcggcacggc tcgcggcccg ctgcatggcg tgccggtggc
     1081 gcacaaggac atctacttca cgcggggcgt caggaccacc gcgggcagcc gcgcgctgga
     1141 cgaccacgtg cccgcgtacg acgcgaccgt ggtcgagctc ctcgccgccg ccggcgccgt
     1201 cagcctcggc aaggccaact gccacgagct cgcctgcggc gccatgcaag gcttcggcgt
     1261 caccaggaac ccttgggacc cgggccgcgc ggcgggaggc tcgagctcag ggtcggcggc
     1321 cgccgtcgcc ggccgcgtcg tcatggcggc tacggcaagc gacacgggcg gttccgtcag
     1381 ggtgcccgcg tccttcaccg gcaccgtcgg cctgaagccg acgttcgggc gcgtcagccg
     1441 gcacggcctg ttcccgatca gttacacgct cgaccaccct gcgctcctca cgaactgcgt
     1501 ggcggacgcg gcaaccgtgc tcggcgccat gtccgcgccg gattccaagg acaggacgac
     1561 cctggcgagg catgacgagg cgcgctggcc ggccttcgcc gacgacctcg cgggcctgcg
     1621 gttgggcgtc cccgccgccc tcctcgcctc gggcctcgac cccgaggtgg aggcggcgct
     1681 cgccgccgct gcgaggcggc tcgaggagct gggggcgacg ctcgtgccgg tggaagtggc
     1741 ggggttcgac ctcgtcgccc ccacgcactc ggtcatctgg ttggcggaag gggctagccg
     1801 gctgcgcggg ctcatcgcgc gtaggcgggc cagcgtcggg gccgtggcgg ccaggcggat
     1861 gctgcccggc ctcctgctga cggcgaacga ctatttcctg gccatgcacg tgagggagcg
     1921 cctgaaagag tccatgcaga agctgttcgc tacggtggac gtgctcctgc tgccgaccac
     1981 gccgtt
//
LOCUS       DAOQYE010000399         1985 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_66817_length_1985_cov_2.810363, whole genome shotgun sequence.
ACCESSION   DAOQYE010000399 DAOQYE010000000
VERSION     DAOQYE010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66817_length_1985_cov_2.810363"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..645
                     /locus_tag="PKN52_07075"
     CDS             <1..645
                     /locus_tag="PKN52_07075"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNQ99746.1"
                     /translation="FAKATGSPTYTANTTIPFSVSHNVDHPVSLYAATKKANELMAHT
                     YSHLYRLPTTGLRFFTVYGPWGRPDMAYFLFTKAILEGRTIDVYNRGDMRRDFTYVDD
                     VVEGVVRTAQMTATPNPEWNGADPDPGTSNAPYRVYNIGNNQPVQLTRFIGIIEKELG
                     IKAKKNYLPMQPGDVHETFADVDALAKDVGFRPSTPLEVGIRRFVQWYREYYGA"
     gene            780..1985
                     /locus_tag="PKN52_07080"
     CDS             780..1985
                     /locus_tag="PKN52_07080"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNQ99747.1"
                     /translation="MSRETFLPFSPPAIGQAELDEVAATLRSDWITTGPRTKAFEAKF
                     GAYVGAPGGTSLMLNSCTAGLHVALVALGVRPGDEVIVPTHTFAATANVVEHVGATPV
                     LVDVLPDTLCIDPAAVERAITPRTRVVMPVHYAGHPADLDPIFELADRHGFEVVEDAA
                     HAAPTYYKDRMVGSRDNFASFSFYATKNLTTAEGGALTGRPDLLDRARVIGLHGMSRD
                     AWKRFDKSGSWDYDIEMPGFKYNMTGVQAALGLHQLDRLSGFHARRVEVVNNYNEAFG
                     RDERFIVPTEREYAKSAWHLYVLRLNLEELSVDRNEFVAELSRRNIGSSVHYRPLHMM
                     SYYAKKFGFAPEDFPVAHAAFKQIVSLPLHPRLTDSDIADVVEAVRGAARAASMQSPR
                     LAKTNSFGG"
BASE COUNT          385 a          658 c          579 g          363 t
ORIGIN      
        1 ttcgccaaag cgaccggttc gccgacctac accgccaaca ccaccatccc gttctccgtg
       61 tcccacaacg tcgaccaccc cgtgagcctc tacgcggcca cgaagaaggc caacgagctc
      121 atggcgcata cgtactcgca tctctaccga ctccccacca ccgggctgcg gttcttcacc
      181 gtctacggtc catggggaag gccagacatg gcgtacttcc tgttcaccaa ggcgatccta
      241 gagggacgaa ctatcgacgt gtacaaccgt ggcgacatgc ggcgcgactt cacctacgtc
      301 gacgatgtcg tcgaaggggt cgttcgcacg gcgcaaatga ctgccacacc gaatcccgag
      361 tggaacgggg cggatcccga ccccggcacg agcaacgccc cttaccgcgt ctacaacatc
      421 ggcaacaacc agcccgtgca gttgacgcgc ttcatcggca tcatcgagaa ggaactgggc
      481 atcaaggcga agaagaacta cttgcccatg cagcccgggg acgtgcacga gaccttcgct
      541 gacgtcgacg cgttggccaa ggacgtcggg tttaggccga gcactccact tgaggtgggc
      601 atccgtcggt tcgtccagtg gtaccgcgag tactatggcg cgtagtgatg tcggtggtcc
      661 gcccaccgat cgttgatcac ccagcattca gtggctcgta agactcgggc cttggacttg
      721 gctggtagca gcaggtccgg gttcccggcc ctgcgctgca aggctattag gagacagtga
      781 tgtcgcgcga gacgttcctt ccattttcgc cccccgccat cggtcaggct gagctcgacg
      841 aggtggcggc caccctacgg tcggactgga tcacgaccgg accccggacc aaagcgttcg
      901 aggctaagtt cggcgcgtac gtgggggcgc caggcggcac gtctttgatg ctcaactcct
      961 gcactgctgg cctacacgtc gcgttggtgg ctttgggagt taggcccggc gacgaggtca
     1021 tcgtgccgac gcacacgttc gccgccaccg ccaacgtcgt cgagcacgtc ggtgccacac
     1081 ctgttctcgt cgatgtcctg ccagacacct tgtgcatcga ccctgccgcc gtcgagcggg
     1141 cgataacccc acgcacccgc gtggtcatgc cggtccatta cgccggtcac ccggccgatc
     1201 tcgaccctat cttcgagctg gcagacagac acggattcga agtcgtagag gacgctgccc
     1261 acgctgcccc cacctattac aaggacagga tggttggcag ccgcgataac ttcgcatcgt
     1321 tctctttcta cgccaccaag aacctgacca cggccgaggg cggcgctctt acgggcaggc
     1381 ccgacttgct cgaccgtgca cgcgttatcg gcctgcatgg catgtcgcgc gatgcctgga
     1441 agcgcttcga caagtccgga tcctgggatt acgacatcga aatgccgggc ttcaaataca
     1501 acatgaccgg cgtccaagcc gccctcggcc tccaccaact tgaccgcctc tccggtttcc
     1561 acgctcgccg cgtcgaagtc gtgaacaact acaacgaagc gttcggtagg gatgagcgct
     1621 tcatcgtccc taccgaacgc gagtacgcca agagcgcctg gcacctttac gtgctgcggc
     1681 tgaacctaga agagctgagc gtggaccgca acgagttcgt cgcggagcta tcgcgtagga
     1741 acattggttc gagcgttcac tatcggccgc ttcacatgat gagttattac gccaagaagt
     1801 ttggcttcgc gcctgaggac ttcccggtcg ctcacgcggc gttcaagcag atcgtgtcgc
     1861 tgccgctcca cccccggctc acggacagcg atatcgccga tgtcgtggag gcggtcaggg
     1921 gagccgctcg cgccgccagc atgcaaagtc ccagactggc gaagacgaac agcttcggcg
     1981 ggtag
//
LOCUS       DAOQYE010000400         1983 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_66930_length_1983_cov_2.479253, whole genome shotgun sequence.
ACCESSION   DAOQYE010000400 DAOQYE010000000
VERSION     DAOQYE010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1983
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66930_length_1983_cov_2.479253"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..883
                     /locus_tag="PKN52_07085"
     CDS             <1..883
                     /locus_tag="PKN52_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99748.1"
                     /translation="DSGHDGTLGRDETDPGPAPADGLGEQTGDPPADRLDERRAEDVD
                     QAHPADGDDGPTPAPAGDLGEGLAQLADDDDDDLYDPVATAADVLAAEVAAADEAGAV
                     DQELPVDDTDELVLDTGEREAALASLLEGSGGAPPATEPVDASADAPADASLGESGDA
                     PVGEPSDAHVDASVDAPADASGGESGGAPVTEAVAEPAPTAPAATGDPFGTEAGGTFL
                     LPVDDPRAVEDFLDEMGGEESFWHPRARSAFARPPTIETVIATVVEPGSEGSDDAPSD
                     ASDGLALEEPGNDPPVA"
     gene            complement(890..>1983)
                     /locus_tag="PKN52_07090"
     CDS             complement(890..>1983)
                     /locus_tag="PKN52_07090"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HNQ99749.1"
                     /translation="WEGHDLHLHAANLVSHHAPYSLVSKMRASFVIMGALLGRTGEAR
                     VSMPGGCAFGPRPVDRHIAAFEAIGATIQEEDGDFYAFRHGKLAGTVAFEAPTVGGTQ
                     NIILAAALSAGEVVIENAAREPEVPDLANMLRGMGADIEGAGTSTVRVRGVDRLRGVD
                     YRPIADRIEAGTYLLAAAATRGQITVTDVAPEHMSAVLDALERTGVVVTRGQDSVSID
                     ATGPLRPIDIEAREYPGVPTDLQAPFSAYLATLPGLSHVRDHVYPDRFTHVDELARAG
                     ARLFLEDQCLEIRGGPLKGTAMHAADIRAGGALIVAALAADGLSEVSGMRYVDRGYER
                     VAERLRGLGARVELAVPKKALTATGTYGG"
BASE COUNT          268 a          710 c          741 g          264 t
ORIGIN      
        1 cgactcaggg catgacggaa ccttgggtcg tgacgagacc gacccgggcc ccgctcccgc
       61 cgacggtctt ggcgagcaga cgggcgaccc cccagccgat cgactcgacg agcgtcgcgc
      121 cgaagacgtc gatcaggctc atcccgctga cggtgacgac ggccccactc ccgccccggc
      181 cggcgacctc ggcgaaggcc ttgcccagct cgcggatgat gatgatgatg acctctacga
      241 cccggtcgcc acggccgcgg acgtgctggc cgccgaggtc gccgcggccg acgaggccgg
      301 cgccgtggac caggagctgc cggtagacga tacggatgag ctcgtcctcg acacgggcga
      361 gcgtgaggcg gcgctggcgt cgctgctcga ggggtcgggt ggcgccccgc cggccaccga
      421 gcccgtcgac gcgtcagcag acgcgcccgc agacgcttcc ctcggcgagt cgggcgacgc
      481 gcccgtaggc gagcccagcg acgcacacgt cgacgcgtcc gtcgacgccc ccgctgacgc
      541 atccggcggc gagtccggcg gcgcgcccgt taccgaagcc gtggcggagc ccgccccgac
      601 ggcgcccgcc gcgacggggg acccgttcgg cacggaggcg ggcggcacct tcctactgcc
      661 ggtggacgac ccgcgcgcgg tcgaggactt cctagacgag atgggcggcg aggagagctt
      721 ctggcacccg cgggcgcgct ccgcgttcgc ccgccctcct acgatcgaga ccgtgatcgc
      781 gaccgtagtc gagcccggtt cggaagggtc ggatgacgcg ccgtcggacg cgtcggacgg
      841 cctggcgctg gaagagcccg ggaacgatcc cccggtcgct taacggggct cagcccccgt
      901 aagtgccggt cgcggtcaac gccttcttgg ggacggccag ctccacgcgc gcgccaagtc
      961 cgcgcaggcg ctcagcgaca cgctcgtagc cgcggtcgac gtagcgcatg ccgctgacct
     1021 cgctcagccc atcggccgcg agggcggcca cgatcagggc gccgcccgcc cggatgtcgg
     1081 ccgcatgcat cgcggtgccc ttgagcggtc cgccgcggat ctcgaggcac tggtcttcca
     1141 ggaagagccg cgctccggcg cgcgccaact cgtcgacgtg ggtgaaccgg tcagggtaga
     1201 cgtggtcgcg gacgtggctg agcccaggga gcgtggccag gtaggcggag aacggcgcct
     1261 gcaggtcggt ggggacgccg gggtactcgc gagcttcgat gtcgatgggg cgcagcggcc
     1321 ccgtcgcgtc gatgctcacg ctgtcctggc cgcgcgtcac caccacgccg gtgcgctcga
     1381 gcgcgtcgag gacagcgctc atgtgctcgg gagcgacgtc cgtcacggtg atctgccccc
     1441 gcgtcgcggc ggcggcgagg aggtacgtgc cggcctcgat gcgatcggcg atcgggcggt
     1501 aatcgacgcc gcgcagcctg tcgacgcctc gcacgcggac ggtgctcgtg ccggcgccct
     1561 cgatgtctgc gcccatgccg cgcagcatgt tggcgaggtc cgggacctcc ggctcgcggg
     1621 cggcgttctc gatgacgacc tcgcccgccg acagggcggc cgccaggatg atgttctggg
     1681 tccctccgac ggtcggcgcc tcgaacgcga ccgtgccggc gagcttgccg tggcggaagg
     1741 cgtagaagtc gccgtcctcc tcctggatgg tggcgccgat ggcctcgaag gcggcgatgt
     1801 gccggtcgac cggtcggggt ccgaacgcgc agccgccggg catgctcacc cgcgcttcgc
     1861 ccgtgcggcc taggagcgcg cccatgatga cgaacgaggc gcgcatcttg ctcacgagcg
     1921 agtagggggc gtggtgcgag accaggttgg cggcgtgcag gtgcaggtcg tggccttccc
     1981 acc
//
LOCUS       DAOQYE010000401         1982 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_66977_length_1982_cov_2.704203, whole genome shotgun sequence.
ACCESSION   DAOQYE010000401 DAOQYE010000000
VERSION     DAOQYE010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1982
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66977_length_1982_cov_2.704203"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="PKN52_07095"
     CDS             complement(<1..183)
                     /locus_tag="PKN52_07095"
                     /inference="COORDINATES: protein motif:HMM:NF019614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HNQ99750.1"
                     /translation="MRATGPSAPIRYVALLRGINVGGHVVKMDRLRQVFTDLGLANVR
                     SYINSGNVFFDTSDTDR"
     gene            complement(180..509)
                     /locus_tag="PKN52_07100"
     CDS             complement(180..509)
                     /locus_tag="PKN52_07100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99751.1"
                     /translation="MSTSIDARMLAGTWSGRNLLWLGPGEPVRESATEATVTTAAANA
                     FLVLAYPWSDQGKQHDGVIMARVAAEPGPRARRPPPWRRATSAARRVERDARGGPARR
                     LAARWVP"
     gene            627..1262
                     /locus_tag="PKN52_07105"
     CDS             627..1262
                     /locus_tag="PKN52_07105"
                     /inference="COORDINATES: protein motif:HMM:NF038065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HNQ99752.1"
                     /translation="MPATTLLGSCRLVLALAIIVAIAATLLDTLTRGPVNVFNFFGYF
                     TIQSNLLLAGALIAAAAYDLRGRAKPRSLELYMAYTTTYIVIVGLVYALLLAPLGAAG
                     GVPVPWANFVLHYLTPVAAAVVWLLVGDRRGLRWNRLLGVLVYPTIWLTVVLIRGATD
                     GWVPYPFLDPANGYGSVAVTCVFICVAILGVGALVFWASRLRLLAVGATED"
     gene            complement(1344..1787)
                     /locus_tag="PKN52_07110"
     CDS             complement(1344..1787)
                     /locus_tag="PKN52_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99753.1"
                     /translation="MPAPEERPKYSLLEIERRWLVDLARVGSLARLPFKRLEDAYWPG
                     TRLRLRQVIDPDGRTTYKFGIKYGESARGVQPVTNLYLDKAEYEALAAAGGLRVSKRR
                     YLIAGGCLDVFDIPAGTPAYFEVEFGSEQEAQAYEPPPFVTEEIT"
BASE COUNT          276 a          657 c          651 g          398 t
ORIGIN      
        1 cctgtcggta tcggacgtgt cgaagaagac gttcccgctg ttgatgtacg agcgcacgtt
       61 ggctaggccc aggtcggtga agacttggcg cagccggtcc atcttgacca cgtgcccgcc
      121 tacgttgatc ccgcgcagga gcgcgacgta ccggatcggt gcgctggggc cggtcgctct
      181 catgggaccc atcgtgctgc gaggcgtctc gctgggccgc cgcgggcgtc acgttccacg
      241 cgtcgcgccg cgctcgtagc gcgcctccac ggcgggggcc tcctcgccct cgggccgggc
      301 tccgccgcga cccgcgccat gatcacgccg tcgtgctgct tgccctgatc tgaccagggg
      361 taggccaaca ccaagaacgc gttggccgcc gccgtggtca cggtcgcctc tgtcgccgac
      421 tcgcggacgg gctcaccggg tccaagccag agcaggttgc ggcccgacca ggtgccagcg
      481 agcatgcggg cgtcgataga tgtgctcata gcgcctcctt cgcttggtgt tcctaccacg
      541 gccggactcg acagtcgtag gtaggacatg ggatggccag gatgaagtct acgcaccgtg
      601 gtcgatcgct tgctttacca tgcaccatgc cggccactac tctgctcggt tcgtgcaggc
      661 tcgtcctcgc ccttgcgatc atcgtcgcga tagccgcgac gttgctggac acgctgacgc
      721 gcggacccgt gaacgtcttc aacttcttcg gctacttcac tatccagtcg aacctgctac
      781 tcgccggcgc gctgatcgcc gcggcggcgt acgacctacg tgggagagcg aaaccgaggt
      841 cgctcgagct ctacatggcg tatacgacca cttacatagt gatcgtcggc ctcgtctacg
      901 ccttgctcct ggcccccctg ggagctgccg gcggcgtgcc ggtgccgtgg gcgaacttcg
      961 tcttgcacta cctgacgccg gtggctgcgg cggtggtctg gttactggtc ggggacaggc
     1021 gcgggcttcg ctggaaccgc ctactcggcg tgctcgtcta tccgaccata tggttgacgg
     1081 tggtgttgat ccggggagcc accgatgggt gggttcctta cccgttcctt gaccccgcca
     1141 acggctacgg ctccgtcgcc gtcacatgcg tgtttatctg tgtggcgatc cttggtgtcg
     1201 gagccctcgt cttctgggcc agccgtcttc gtctgttggc ggtcggtgcc acggaggact
     1261 agccgccacg ggcgtgcaag gctcggtgag gcgcatctgc ctacctgtgg tgtgccgcgt
     1321 acctgatggc cgcttgcagg gcttcaggtg atctcctccg tcacgaacgg cggcggttca
     1381 taggcctgcg cctcctgctc cgagccgaac tcgacctcga agtaggcggg cgttccggct
     1441 gggatgtcga acacgtccag gcacccaccg gcgatcaggt acctgcgctt gcttaccctc
     1501 aggcctccgg cggcggcgag cgcctcgtac tccgccttgt cgaggtacaa gttcgtcacc
     1561 ggttggaccc cccgcgccga ttcgccgtat ttgatgccga acttgtacgt cgtgcggccg
     1621 tcggggtcga tgacctgcct gaggcgcaga cgggtgccgg gccagtaagc gtcctcgagg
     1681 cgtttgaacg gcagcctggc gagggagccg acgcgggcca ggtcgacgag ccacctgcgc
     1741 tcgatctcca gcagcgagta cttcggtcgc tcttcggggg ccggcatctt ctcactctct
     1801 gatctgccgc ctcggccccg cggttccggg cggggtggcg gggtgcgact ttgtcgcgcc
     1861 gtgagcctac gttggcctga ccgaccgaac aagtcaagct cacttgacat ccagcgctag
     1921 tcaagtatgc ttgacatgtc aaggacgctt gacggcaggt ggtcgcgacc gtatctggcc
     1981 ag
//
LOCUS       DAOQYE010000402         1981 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_67040_length_1981_cov_2.811007, whole genome shotgun sequence.
ACCESSION   DAOQYE010000402 DAOQYE010000000
VERSION     DAOQYE010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1981
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67040_length_1981_cov_2.811007"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1476)
                     /locus_tag="PKN52_07115"
     CDS             complement(<1..1476)
                     /locus_tag="PKN52_07115"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNQ99754.1"
                     /translation="MTWSTVKRALFVFAALLLAVPAAAQETPVKGGTAIVILGSDPEH
                     LNVGISTSYPVAAVGANIYSGLVRFNAEGRPEGDLASSWTVSDDNLTYTFKLRQANFH
                     DGTPVTSADVVYSMLEIEAPNHGRFKTAFDQIASIEAPDAETVVITLKNPYAPLLSLL
                     SVFDAPVLPKHVFEGTDPLTNPANDAPIGSGPFKFANWVRGERVEIVRNDDYFLDEAL
                     LDKIIFRIVPQDVARSTALEVGEADLLWGFYMPPADLPRLEADPNLNTWTGITIPALY
                     FVFINTNTPGLDDVRVRQAIMYAVDREQVVEQAQGGLGQAGSGPFGAGFPYAYDESTD
                     YRTRYPYNPEKAKELLAEAGVSGLQLRFVYDSARGAFGAAGDIMRDQLRQVGIDLVLQ
                     PAERAVMVDRVYSGDYDLTMQSFTSNADPAIGYHRIYASAAPGTPFVNATGYSNADVD
                     TWLRQAGGLADQDARAAYYRQINEVIANDVPVMVMFDELASE"
     gene            complement(1586..>1981)
                     /locus_tag="PKN52_07120"
     CDS             complement(1586..>1981)
                     /locus_tag="PKN52_07120"
                     /inference="COORDINATES: protein motif:HMM:NF018663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNQ99755.1"
                     /translation="FYLAIFDDARQVSMSGRPGSASGVTIELGGQRLVLFNGGPYFRL
                     NEAFSLSIDCDTQAEVDYYWERLTADGGQESQCGWLKDKFGVSWQVVPKVLMRGLSDP
                     DPAVANRVRESMLKMIKLDVAELERAYSG"
BASE COUNT          311 a          610 c          716 g          344 t
ORIGIN      
        1 ctcggaggcc agctcgtcga acatcaccat cacaggcaca tcgttcgcga tgacctcgtt
       61 gatctggcgg tagtacgccg cgcgcgcgtc ctgatcggcg agaccacccg cctgacgcag
      121 ccaggtgtcc acgtcggcgt tggagtagcc ggtggcgttc acgaaggggg tgcccggagc
      181 ggcgctggcg tagatgcggt ggtagccgat cgccgggtcg gcgttcgagg taaacgactg
      241 catggtgagg tcgtagtcgc cgctatacac gcggtcgacc atgacagcgc gctccgcggg
      301 ctgcagcacg aggtcgatgc cgacctggcg cagttggtcg cgcatgatgt cgccggcggc
      361 gccgaacgcc ccgcgggccg agtcgtacac gaagcgcagc tgcagaccgc tcacgccggc
      421 ctcggcgagg agttccttgg ccttctccgg gttgtacggg tagcgggtgc ggtagtcggt
      481 cgactcgtcg taggcgtacg ggaagcctgc gccgaagggg ccggagccgg cctgcccgag
      541 ccccccttgg gcctgctcga cgacctgctc gcggtcgacg gcgtacatga tggcctggcg
      601 gacgcgcacg tcgtcgaggc cgggggtgtt cgtgttgatg aagacgaagt agagcgccgg
      661 gatcgtgatg cccgtccacg tgttcaggtt cgggtcggct tcgagacgcg gcaggtcggc
      721 cggcggcatg tagaagcccc acagcaggtc ggcctcgccg acctcgaggg ccgtggagcg
      781 cgcgacgtcc tgcggcacga tgcggaagat gatcttgtcc aggagggcct cgtcgaggaa
      841 gtagtcgtcg ttgcgcacga tctcgacgcg ctcgccgcgc acccagttcg cgaacttgaa
      901 gggaccgctg ccgatggggg catcgttggc cgggttcgtg agcggatccg tgccctcgaa
      961 gacgtgcttg gggaggaccg gggcgtcgaa cacggacagg aggctcagca acggcgcgta
     1021 agggttcttc agcgtgatga cgacggtctc ggcgtcgggc gcctcgatgg aggcgatctg
     1081 gtcgaaggcc gtcttgaagc gcccgtggtt cggcgcctcg atctcgagca tggagtacac
     1141 gacgtcggcg ctggtgactg gcgtgccgtc gtggaagttc gcctggcgca gcttgaaggt
     1201 gtaggtcagg ttgtcatccg acaccgtcca gctgctcgcg aggtcgcctt cgggacgccc
     1261 ttccgcgttg aagcgcacga ggccgctata gatgttggcg ccgacggcgg ccacggggta
     1321 gctcgtgctg atgccgacgt tgaggtgctc cgggtcggag ccgagtatga cgatggcggt
     1381 gccgcccttg acgggcgtct cctgcgcggc ggccggaacg gctagtagca gcgcagcgaa
     1441 cacgaacagt gcgcgcttga cggtcgacca ggtcatcggg gaacctgcct ttcactagtg
     1501 aggatgctcg gaggggagcg gtagctccct agtgacgaat ctagcatccg ggcgcctcgt
     1561 cgacgtgcgg gcgcgcgggc agtgcctatc cggagtacgc cctctcgagt tcggccacgt
     1621 caagcttgat catcttcagc atgctctccc tgacgcggtt cgccaccgct gggtcggggt
     1681 ccgagaggcc gcgcatgagg accttcggca cgacctgcca ggagaccccg aacttgtcct
     1741 tcagccagcc gcactgcgac tcctgcccac cgtccgccgt caggcgttcc cagtagtagt
     1801 ccacttcggc ctgcgtgtcg cagtcgatcg agagcgagaa ggcctcgttg agtcggaagt
     1861 aggggccgcc gttgaagagg acgaggcgct gcccacccaa ctcgatcgtg acgcccgatg
     1921 cgctgcctgg ccgtccgctc atggatacct ggcgtgcgtc gtcgaagatg gccaagtaga
     1981 a
//
LOCUS       DAOQYE010000403         1977 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_67244_length_1977_cov_3.632674, whole genome shotgun sequence.
ACCESSION   DAOQYE010000403 DAOQYE010000000
VERSION     DAOQYE010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67244_length_1977_cov_3.632674"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..756
                     /locus_tag="PKN52_07125"
     CDS             <1..756
                     /locus_tag="PKN52_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99756.1"
                     /translation="VTVEGHPDSSVGLFSVGVSGGFPSYQTVALAVSVQAQFVGGQLK
                     ASWTPAGVYLGGQLRAYPPIPIPLPVYLGVGGGVYGSQFAYHFALGTHVPLGANLRLD
                     LEGGVANVPSLGERAWVPHLAAGISYAFPVELSPGEPLAASGTVGAPAAGAACTTTRE
                     PDPDGIAGVIRATVNDWILSARATYGSVYTDLSYKYSITSVRRTGTTANVAVAYSGSV
                     REILSGARHSASGTAQVRLVWSGCGWGVTSVEY"
     gene            complement(871..>1977)
                     /locus_tag="PKN52_07130"
     CDS             complement(871..>1977)
                     /locus_tag="PKN52_07130"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNQ99757.1"
                     /translation="PKSHLALALSPKGPIVTTIPTLPGITSTRVATPRLTSHVLTSGP
                     DDGTPVLFVHGNASSSTFWEETMLALPGGYRAIAPDLRGYGDTDDVLVDATRGVGDWV
                     DDLLALKAALGIGRYHAVGHSLGGSVLWGLIAADGANIVSATVIAPGSPFGFGGTKGL
                     EGTPCFPDFAGTGGGSVNPDFAARMGAGDRSSESGTSPRTVMNTFYWKPPFKAEREEE
                     LLSELLSEKVGPQKYPGDSVPSANWPGIAPGVWGPANAISAKYVGDGVERLIALQSKP
                     RVLWVRGVDDQIVSDESLFDLGTLGKLGAVPGWPGEDVFPPQPMVGQTRAVLERYRAA
                     GGAFEEVALADTGHTPYLERPREFMAALVAHLGG"
BASE COUNT          257 a          702 c          725 g          293 t
ORIGIN      
        1 gtcacggtgg agggccaccc cgactcgagc gtcggcctct tctcggtggg cgtctcagga
       61 gggttcccgt cgtatcagac ggtcgccctt gccgtgtccg tgcaggccca gttcgtgggc
      121 ggccagttga aggcgagctg gacgcccgcc ggcgtctacc ttggcggcca gttgcgcgcc
      181 tacccgccga tcccgatccc gctgcccgtc tacctgggcg tcggcggggg cgtgtacggg
      241 tcgcagttcg cctaccactt cgcgctcggc acgcacgtgc cgctcggcgc gaacctccgg
      301 ctcgacctcg agggcggcgt ggcgaacgtg ccgagcctcg gcgagcgcgc ctgggtgccg
      361 cacctcgcgg cgggcatcag ttacgcgttc ccggtcgagc tctcgcccgg cgagcccctc
      421 gccgcctccg gcacggtcgg ggccccggca gccggcgccg cctgcacgac gacgcgcgag
      481 cccgacccgg acggcatcgc gggcgtgata agggcgaccg tgaacgactg gatcctcagc
      541 gcgcgcgcta cctacggcag cgtctacacc gacctgagct acaagtactc gataacgagc
      601 gtcaggcgga ccggcacgac ggccaacgtg gccgtcgcct actcgggcag cgtccgcgag
      661 atcctgagcg gcgcgcggca cagcgcgtcg ggcacggcgc aggtccgcct cgtgtggtcc
      721 gggtgcggct ggggagtgac cagcgtcgag tactgactcg ggcggacgta gcgcgcgtcg
      781 gcctcggcgc cggtgagcgt gagtggcctg ggcctcgtgg tgcgtaacac gcgcggcgtc
      841 ccttcagttg cggcgccccg tccgccgctc ctaccctccc agatgcgcga cgagcgccgc
      901 catgaactcg cgcggccgct cgaggtacgg cgtgtggccg gtgtccgcca gcgcgacctc
      961 ctcgaacgcg ccgcccgcgg cgcggtagcg ctccaggacg gcgcgcgtct gcccgaccat
     1021 gggctggggc gggaacacgt cctcgcccgg ccagcccggc acggcgccga gcttccccag
     1081 cgtgcccagg tcgaagagcg actcgtccga gacgatctga tcgtcgacgc cgcgcaccca
     1141 tagcacgcgc ggcttcgact gcagggcgat gagcctctcg acgccgtctc cgacgtactt
     1201 ggccgagatg gcgttggccg ggccccatac gcccggcgcg atgccgggcc agttggccga
     1261 cggcacggag tcgcccgggt acttctgcgg tccgaccttc tcgctcagca gctcggagag
     1321 gagctcctcc tcacgttcgg ccttgaaggg cggcttccag tagaacgtgt tcatgaccgt
     1381 gcggggcgac gtgccgctct cgctcgagcg gtcgcccgcg cccatgcgcg ccgcgaagtc
     1441 cgggttgacg gacccgccgc ccgttccggc gaagtcgggg aagcacggtg tgccctccag
     1501 gcccttggtg cccccgaagc cgaacggcga gcccggcgcg atgacggtgg cggagacgat
     1561 gttcgcgccg tcggccgcga tgagccccca caggaccgat ccgccgagcg agtgaccgac
     1621 cgcgtggtag cggcctatcc cgagtgccgc cttgagggcg agcaggtcgt ccacccagtc
     1681 gccgacgccg cgggtggcgt ccaccagcac gtcgtcggtg tcgccgtagc cccgtaggtc
     1741 gggggcgatg gcccggtagc cgccgggcag tgccagcatg gtctcctccc agaacgtgct
     1801 tgaggaggcg ttgccgtgca cgaacagcac gggcgtgccg tcgtccgggc cggacgtgag
     1861 gacgtggctg gtgaggcggg gggtggcgac gcgcgtcgac gttatgcctg ggagggtcgg
     1921 gatggtggtc acgatgggtc ctttcggtga tagtgcgagc gctaaatgcg acttcgg
//
LOCUS       DAOQYE010000404         1977 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_67285_length_1977_cov_1.799168, whole genome shotgun sequence.
ACCESSION   DAOQYE010000404 DAOQYE010000000
VERSION     DAOQYE010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67285_length_1977_cov_1.799168"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1501
                     /locus_tag="PKN52_07135"
     CDS             <1..1501
                     /locus_tag="PKN52_07135"
                     /inference="COORDINATES: protein motif:HMM:NF013132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HNQ99758.1"
                     /translation="CTPVSPFQLMLGKLLLPVAYAALVGGMLTLIATPPNLVVAQALE
                     GAGRPPFGFFRFTPLGVAMLAVATGYMALVGRRMLPAAAPSTPGDAGRAGSDDLSVVA
                     ERFGLTRLKRLTLAPGSPVVGSDLGALAWPERHGVRVLAVDTEPAAVRSGRRSRRHLG
                     RSKRLGPDTALSEGDRMLLQGSQEGLAAAAAEAGAPLETVDGLEGTVPPNLAFAEVLP
                     TPRSGWLGRTLAELRFRERFRVQVVALRRGQEQVTEGGGALRLAETRLRFGDTLLVQG
                     TLAAIARLGGERLDAVVLSEAGGEREAPKRRLAPVAAAIMLLMLVSMSAGWLEPVVAV
                     LLAALALVAARCLSAEEAYRSIQWQAVVLITGMLPLATAFELTGGVRLVADGVVAAFG
                     GSGELLLLAGIYLLTATLGQLMSNTATAVLIAPIALSASTSVGVAPESALMMVALASA
                     ASFCTPMATPVNTLVVGPGAYRFRDFVRVGLPLQVLMAAVGLVLIPWLF"
     gene            complement(1535..>1977)
                     /locus_tag="PKN52_07140"
     CDS             complement(1535..>1977)
                     /locus_tag="PKN52_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99759.1"
                     /translation="IAYHHLPVFHTEHCVFCRFLSTGHDFRDCGHPCETHRLALRDAR
                     GRQHPVLADVGCRNTVFSGEAQVAAKHLGAWWDAGIRDYRLEFVHEDAAAVGAVTRAF
                     AAALAATATGGYRPAQLEGRLTDSVGTITEGSLYVPRRANAITI"
BASE COUNT          230 a          699 c          735 g          313 t
ORIGIN      
        1 ttgtacgccg gtgagcccgt tccaactgat gctcggcaag ctcctcctgc ccgtggccta
       61 cgcggccctc gtcggcggca tgctcaccct catcgccacc ccgcccaacc tggtcgtggc
      121 gcaggccctc gagggcgctg gtcggccacc gttcggcttc ttcaggttca cgccgttggg
      181 agtggcgatg ctcgccgtgg cgaccggcta catggcgctc gtggggaggc gcatgctgcc
      241 ggccgccgcg ccgtccacgc ccggcgacgc gggccgcgcg ggaagcgacg acctgagcgt
      301 cgtggcggag cggttcgggc tcacgagact gaaacggctg acgttggcgc ccggctcgcc
      361 cgtggtcggg tccgacttgg gcgccctggc ttggccggag cgccacggcg tgcgcgtgct
      421 cgcggtggac acggaaccgg ccgctgtgcg cagcggcagg cgctcgcgcc gccacctcgg
      481 gcgctccaag cggctcggcc cggacacggc gttgagcgag ggcgaccgta tgctcctgca
      541 ggggagccag gaagggctgg ccgccgccgc tgcggaggcg ggcgcgccgc tcgagacggt
      601 cgacggtctg gaaggtacgg tgccgccgaa cctcgccttc gccgaagtgc tcccgacgcc
      661 ccgctccggc tggctcggac gcaccctggc agagctccgc ttccgcgagc gcttccgggt
      721 gcaggtcgtc gcgttgcgac gcggtcagga acaggtgacc gagggcggcg gcgccctgcg
      781 cctggccgag acgcgactgc gcttcggcga cacgctcctc gtccagggga ccttggcggc
      841 gatcgcgcgc ctgggcggcg agcgcctcga tgccgtggtg ctcagcgagg cgggcggcga
      901 gcgcgaggcc cccaagcgac gcctcgcccc ggtcgcggcc gccatcatgc tgctcatgct
      961 cgtgagcatg tcggcgggct ggctcgagcc cgtcgtggcc gtgctgctgg cggccctggc
     1021 gctcgtcgcc gcgcgctgcc tctcggcgga ggaggcctac cgctccatcc agtggcaggc
     1081 ggtggtcctc ataacgggca tgttgccttt ggcgacggcg ttcgagctga ctggtggggt
     1141 gcggctcgtg gcggacgggg tggtcgcggc gttcggcggc tcgggtgagc tcctgttgtt
     1201 ggccggcatc tacctgctga cggctaccct cggtcagctc atgtccaaca cggccaccgc
     1261 ggtgctgata gcgccgatcg ccctcagcgc ctcgacctcg gtcggcgtcg cgcccgagag
     1321 tgccctgatg atggtggcgc tcgcgtcggc cgcgtccttc tgcaccccga tggcgacgcc
     1381 cgtgaacacc ctggtcgtgg gcccgggcgc ctatcgcttc cgcgacttcg tgagggtggg
     1441 tttaccgctc caggtcctga tggctgccgt aggcctcgtg ctcatcccgt ggctgttcta
     1501 gagcgcccgg ggcctgcctg gagcgtgatc gtgcctagat cgtgatcgcg ttcgccctgc
     1561 gcggcacgta caggctgcct tccgtgatgg tgccgaccga gtcggtcagg cggccctcga
     1621 gctgcgccgg acggtagcca ccggtcgccg tcgccgccag cgccgccgcg aacgcgcgtg
     1681 tcaccgcccc gacggccgcg gcgtcctcat ggacgaactc caggcggtag tcgcggatgc
     1741 ccgcgtccca ccatgcgcca agatgcttcg ccgccacctg cgcctcgccg ctgaagaccg
     1801 tgttgcggca gccgacgtcg gcgagcaccg gatgctggcg gccgcgcgcg tcgcgcaagg
     1861 cgaggcggtg cgtctcgcac gggtggccgc agtcgcggaa gtcgtggccg gtcgacagga
     1921 accggcagaa gacgcaatgc tcggtatgga agacgggcag gtggtgatag gcgatca
//
LOCUS       DAOQYE010000405         1975 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_67381_length_1975_cov_2.644792, whole genome shotgun sequence.
ACCESSION   DAOQYE010000405 DAOQYE010000000
VERSION     DAOQYE010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1975
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67381_length_1975_cov_2.644792"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1975)
                     /locus_tag="PKN52_07145"
     CDS             complement(<1..>1975)
                     /locus_tag="PKN52_07145"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF012532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HNQ99760.1"
                     /translation="RGLDLQLTVTAHPTEVKRYTVRLKLERIGEALRRLHETELAPRA
                     RKVLTDSIYAEIATLWLTREVAMERPSVLDEVKSALYYFRRSILDVVPRLMAELEEAL
                     DAYFPDGGVGRADHRGPPSEAPVPVLRFRSWIGGDRDGNPYVTPEVMREAFALHAAVA
                     NEAYIADVNGLVQRFSQWDGRTMLTQEFRAALAERDDSEGRSPRFEGEPYRRWLEHVY
                     KALVREGADPGTYPGGEPGYRADLARLETALRFGHGGRPADAFVRPTMRRAAAFGFPL
                     APLDIREHSRVHEMAVASVLAAGAAHPDYLSLPEAQRVALLARELGSPRPLLGPRASV
                     AAEAERALAFLAEFRRAADRHGDDAHGSTIVSMTEGASDVLEALLLAKEAGVTEIEAT
                     PLFETLADLRAAPRVMRDLFAVPEYLAHVRRRGVQEVMIGYSDSNKDVGFVSASWALY
                     VAQEGLAEVCREAGVPLRIFHGRGTSIGRGGGPAGAAILAQPPGSLAGRMRLTEQGEA
                     LADRYADPDLAHRHLEQVMHAFILASARDARALPEVEPRYRTAMDAGADAAMARYRAL
                     VQGEGFMEFFKQVTPIEELAQLNLGSRPTRRAGAPSMANLRAIPWVFAFTQCRANLPG
                     WYGLGAALAAIGPELGREMYRAWPFFTTMIDFA"
BASE COUNT          272 a          698 c          734 g          271 t
ORIGIN      
        1 tgggcgaagt cgatcatcgt ggtgaagaac ggccaggcgc ggtacatctc gcgcccgagc
       61 tccggaccga tcgccgcgag cgccgccccg agtccgtacc agcccgggag gttcgccctg
      121 cactgggtga aggcgaacac ccaggggatg gcgcgcaggt tggccatgct cggggcgccg
      181 gcgcggcgcg tggggcgcga gcccaggttg agctgggcga gctcctcgat gggcgtcacc
      241 tgcttgaaga actccatgaa cccctcaccc tgcacgagcg cccggtagcg ggccatcgcc
      301 gcatcggcgc cggcgtccat ggcggtccgg tagcgcggct cgacctcggg gagcgcgcgt
      361 gcgtcgcggg cgctggcgag gatgaaggcg tgcatgacct gctcgaggtg gcgatgcgcc
      421 aggtccggat cggcgtagcg gtcggccagc gcctcgcctt gctccgtgag gcgcatgcgc
      481 ccggccaggc tgccaggggg ttgagcgagg atggccgcgc cagcgggccc ccctcccctc
      541 ccgatgctag tgccgcgccc gtggaagatg cgcagaggca cgccggcctc cctgcacact
      601 tccgccaggc cctcctgcgc cacgtacaag gcccaggagg ccgacacgaa gcccacgtcc
      661 ttgttggagt cggagtagcc gatcatcacc tcctgcacgc cgcggcgccg cacgtgcgcg
      721 aggtattcgg ggaccgcgaa gaggtcgcgc atgacgcggg gcgccgcgcg caggtcggcg
      781 agcgtctcga agaggggcgt agcctcgatc tccgtgaccc ccgcctcctt ggcgagcagc
      841 agggcctcca ggacgtcgga tgccccctcg gtcatgctga cgatcgtgct gccgtgggcg
      901 tcgtcaccgt gccggtcggc cgcgcggcgg aactccgcca ggaaggccag cgcccgttcc
      961 gcctcggcgg cgacgctcgc gcgggggccg agcagcggcc gcggcgagcc cagctcgcgg
     1021 gcgagcaagg cgacccgctg cgcttcgggt agcgacaggt agtcggggtg ggcagcgccg
     1081 gccgccagga cgctggcaac ggccatctcg tgcacacggc tgtgctcgcg gatgtcgagc
     1141 ggggcgagcg gaaagccgaa cgcggccgct cggcgcatgg tcggacgcac gaaggcgtcg
     1201 gccggccggc ccccgtgccc gaagcgcagc gccgtctcga ggcgcgccag gtcggcgcgg
     1261 tagccgggct cgccgcccgg gtaggtgccg gggtcggcgc cttccctgac caacgccttg
     1321 tacacgtgct ccagccaccg acggtacggc tcgccctcga agcgcgggct gcggccctcc
     1381 gagtcgtcgc gctcggcgag cgcggcgcgg aactcctggg tcagcatcgt cctgccgtcc
     1441 cactgcgaga aacgctgcac caggccgttg acatcggcga tatacgcctc gttggcgacg
     1501 gctgcgtgaa gggcgaacgc ctcgcgcatg acctcagggg taacgtaggg gttgccgtcg
     1561 cggtcgccgc cgatccagga gcggaagcgc aggaccggca cgggcgcctc gctcggtggg
     1621 ccacggtggt cggcgcggcc gaccccgcca tccgggaagt aggcgtcgag cgcctcctcg
     1681 agttcggcca tcaggcgcgg caccacgtcg aggatcgagc gcctgaagta gtacagagcg
     1741 ctcttgacct cgtcgaggac gctcggtcgc tccatggcca cctcgcgcgt tagccagagc
     1801 gtggcgatct cggcgtagat gctgtccgtg agcaccttcc tcgcgcgcgg cgccagctcc
     1861 gtttcgtgca ggcgcctgag cgcctcgccg atgcgctcga gcttgaggcg aaccgtgtag
     1921 cgcttcacct ccgtcgggtg cgccgtgacg gtgagctgca ggtcgagccc gcgca
//
LOCUS       DAOQYE010000406         1972 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_67559_length_1972_cov_2.002087, whole genome shotgun sequence.
ACCESSION   DAOQYE010000406 DAOQYE010000000
VERSION     DAOQYE010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1972
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67559_length_1972_cov_2.002087"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="PKN52_07150"
     CDS             complement(<1..225)
                     /locus_tag="PKN52_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HNQ99761.1"
                     /translation="MKRTHNCGDLRASDAGATVTLQGWVNRRRDLGGLVFLDLRGRGG
                     LTQVVVEPDDAAAFAAAEDVRNEYVVEVTGV"
     gene            complement(242..1519)
                     /gene="hisS"
                     /locus_tag="PKN52_07155"
     CDS             complement(242..1519)
                     /gene="hisS"
                     /locus_tag="PKN52_07155"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HNQ99762.1"
                     /translation="MRLQAVKGTQDILPRDQAAWSSVTAAAKAVLEAAGAGALTTPIF
                     EHAEVFEKSVGDSADLVVQKEMYTFEDRGGRRLTLRPEFTAGVMRAFIENGMHTLPHP
                     VKLWSHGPLFRAENVQRGRQRQFHQVNYESIGLDSPLADAEAIELLYRTLRLCGLTRH
                     RVKLGSVGDPEDRQAYNAYLRAQLADVAHQLSPVSQERLRLNPMRVLDSKDANDQKLI
                     AHLQRPLDRLGPEARAHLAKVEEYLTAWGVPFDLEPAIVRGLDYYRRTAFEAHYEGIG
                     AQSALGGGGRYDGLIEMLGGPALPATGWAFGVERVLDALSQEAERPAHDAPRPDLFLV
                     PLDEAAVDEAALLATSLRRAGRWVEYAYQRRNPGKGLRDADRSKARYAAVRGADERQR
                     RAWQLKDLATGEQSEVAETDLADALAERLPEHA"
     gene            complement(1635..>1972)
                     /locus_tag="PKN52_07160"
     CDS             complement(1635..>1972)
                     /locus_tag="PKN52_07160"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HNQ99763.1"
                     /translation="GLVAAVMTSCTPRPSVVTNVPVTLISASTPTTKGGNIVLQGRYF
                     GDGRTGGDEANRVIVGANAEGSGGQTAQVVEWSPTRIVLVAPETGGNGWVFVVAGGIR
                     SNGLPLNLP"
BASE COUNT          265 a          656 c          735 g          316 t
ORIGIN      
        1 gacgccggtc acctcgacga cgtactcgtt gcgcacgtcc tcggccgccg cgaaggccgc
       61 ggcgtcgtcg ggttcgacca ccacctgcgt gaggccgcca cggccgcgga gatcgaggaa
      121 cactaggccg ccgaggtcgc ggcggcggtt cacccaccct tgcagggtga cggtggcgcc
      181 ggcgtcggaa gctcgcaggt ctccgcagtt atgggttctc ttcatgtcct agggtcgcct
      241 ttcaggcgtg ctcgggcagg cgctcggcga gcgcgtccgc caggtcggtt tccgccacct
      301 cggactgctc gccggtggcg aggtccttga gctgccaggc gcggcgctgg cgctcgtccg
      361 cgccgcggac cgcggcgtag cgggccttgc tccggtcggc gtcgcgcagg cccttgcctg
      421 ggttgcggcg ctggtacgcg tactcgaccc atcgacccgc ccgccgcagg gaggtggcca
      481 ggagggcggc ctcgtcgacc gccgcctcat cgagcggcac gaggaagagg tccgggcgcg
      541 gcgcatcatg agcggggcgt tcggcctcct gcgacagcgc gtcgaggacc ctctcgaccc
      601 cgaacgccca gccggtggcg ggcagcgcgg ggccgccgag catctcgatg agcccgtcgt
      661 agcggccgcc gccgccgagc gccgactgcg cgccgatgcc ctcgtaatgg gcctcgaagg
      721 cggtgcgtcg gtagtagtcc agtccccgca ctatggcggg ctcgagatcg aacggcacgc
      781 cccacgcggt gaggtactcc tcgaccttgg cgaggtgggc gcgcgcctcc gggccgagcc
      841 ggtcgagtgg acgctgcagg tgggcgatca gcttctggtc gttggcgtcc ttgctatcga
      901 gcacgcgcat ggggttgagg cgcaggcgct cctggctgac ggggctgagc tgatgcgcga
      961 catcggccag ttgggcgcgc aggtaggcgt tgtaggcctg cctgtcctcc gggtcgccga
     1021 cggagccgag tttcaccctg tggcgcgtga ggccgcagag ccgcaacgtg cggtagagga
     1081 gctcgatggc ctcggcgtcc gccagagggc tgtcgaggcc gatcgactcg tagttgacct
     1141 gatggaactg gcgttggcgt ccgcgctgca cgttctcggc gcggaagagc ggaccatggc
     1201 tccagagctt cacggggtgg gggagcgtgt gcatgccgtt ctcgatgaac gcgcgcatca
     1261 cgccggccgt gaactccggc ctgagggtga ggcgcctgcc cccgcggtcc tcgaaggtgt
     1321 acatctcctt ctggaccacc aggtcggccg agtcgccgac ggacttctcg aacacctcgg
     1381 cgtgctcgaa gatcggggtg gtgagcgcgc cggcgcctgc ggcctcgagc accgccttgg
     1441 ccgcggcggt cacgctcgac caggccgcct ggtctcgcgg aaggatgtct tgggtacctt
     1501 tgactgcctg gagtctcatc ctggcagagt gtacacctgg cgcctggagg cccaagagcg
     1561 cggggctggc agcatccgct gccagccccg cgaagaagct gtggtgagga ggaggcccgg
     1621 aggctactac gaactcaagg gaggttcagt gggaggccgt tgctcctgat accgccggcc
     1681 actacgaaga cccagccgtt gccgcccgtc tcgggcgcga ccagcacgat gcgggtcggg
     1741 gaccattcga cgacctgggc ggtctggccg ccgctgccct cggcgttggc gccgacgatg
     1801 acacggttgg cttcgtcgcc gcccgtgcga ccgtcaccga agtagcgccc ctggaggacg
     1861 atgttgccgc ccttggtggt cggcgtggac gcggagatga gggtgacggg cacgttcgtg
     1921 acgacggacg gccgcggcgt gcaactggtc atgacggccg ctactaggcc ta
//
LOCUS       DAOQYE010000407         1971 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_67588_length_1971_cov_2.844468, whole genome shotgun sequence.
ACCESSION   DAOQYE010000407 DAOQYE010000000
VERSION     DAOQYE010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1971
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67588_length_1971_cov_2.844468"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1296
                     /locus_tag="PKN52_07165"
     CDS             <1..1296
                     /locus_tag="PKN52_07165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99764.1"
                     /translation="AALAAAPPAEDGPTVVQVKGTSGALEATRALTFDVESLALVGHV
                     VSLLGTPLANVTAQSQGKTAVTNAAGNFYLAGLAGPYDLTLSSAADVWTHVYEGLTGD
                     TLTFLPDGAPLPSLGSGSMQFTGSLSGGSIPVPASHQVRVCAEGVDAIVIGCTTVAAG
                     LSAYSLTTYWFDGSPQVRLHAFEIELDGDKPVDYIAYGTATATVVDGVAQVLDLTLGA
                     GLSSFDYSVEATVPTGYPTPTLNVLLRLGDHHWSILQTVASTYASVKVPVLDGVSASV
                     LAGSAVGPNNINFGWGQVTTTQIAIPDTPVLGAPPDAATNVTSDTAFSAGFAPGQIPT
                     YVWGPQTDGAGPTYALTTTRTDVRIPAPDAGPLALPPGIPYEWVAYGVVANDVDHATT
                     SPGRGLLVQALATLSGSSWGFEGDGGLAMGESREFTTAP"
     gene            complement(1402..>1971)
                     /locus_tag="PKN52_07170"
     CDS             complement(1402..>1971)
                     /locus_tag="PKN52_07170"
                     /inference="COORDINATES: protein motif:HMM:NF020939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HNQ99765.1"
                     /translation="LIDVLLGHATERVQSLGHDRLSTFGIGTELDERAWRSVARQLLA
                     NGQLLPDPDGFGGLVLGPGAAAVLRGEVSVELRRDRERAKPSTRRQRGRGLAGGAPGS
                     SSFASADSTDAAGPAEEALFQALRALRLEIAREQGVPPYVVFHDKTLREMVASEPRSL
                     AELALVPGIGVAKLERYGERFLELLVSHR"
BASE COUNT          299 a          730 c          650 g          292 t
ORIGIN      
        1 gcggcgctcg ccgccgcgcc cccggccgag gatgggccaa cggtcgtgca ggtgaaaggt
       61 acttcgggcg ccttggaggc cacgcgggcc ctcaccttcg acgtagagtc cttggcccta
      121 gtgggtcatg tcgtgtccct gcttggcacg ccgctggcca acgtcacggc tcagagccaa
      181 ggcaagaccg ccgtcacgaa cgccgccggt aacttctacc tcgctgggct ggccgggccg
      241 tacgacctga cgttgtccag cgccgccgac gtgtggactc atgtctacga gggcctgacc
      301 ggcgatacgc tcacgttcct gcccgacggc gcgccactgc catcgctggg ctcagggtcc
      361 atgcagttca ccggttccct cagcggcgga tccatcccgg tgcccgcctc gcaccaggtg
      421 cgcgtgtgcg ccgagggggt ggatgccatc gtcatcggct gcaccaccgt cgccgcgggg
      481 ctgtccgcct actcgctgac cacctactgg ttcgacggtt cgccgcaagt gagactgcat
      541 gcgttcgaga tcgagctgga cggcgacaag cccgtcgatt acatcgccta cggcacggcc
      601 acggctacgg tggtcgacgg cgtggcccag gtgcttgacc tgaccctggg cgcgggactc
      661 tcgagcttcg actattccgt cgaggccacc gtgccgaccg gctaccccac accgacgctc
      721 aacgtcttgc ttcggctcgg cgaccaccat tggtcgatcc tgcagacggt cgccagcacg
      781 tacgcgtctg tcaaggtgcc ggtgctggac ggggtctcgg ccagcgtgct ggctggctcc
      841 gccgtggggc cgaacaacat caacttcggt tgggggcagg tcaccaccac gcagatcgcc
      901 atccccgaca ccccagtgct cggagcgccc cccgacgccg cgaccaacgt cactagcgac
      961 accgccttca gcgccggctt cgccccagga cagatcccca cctacgtctg ggggccgcag
     1021 acagacgggg cggggccgac gtacgctctg accaccactc ggacggacgt caggatcccc
     1081 gcgcccgacg ccggtcccct cgcactcccg cccggcatcc cgtacgagtg ggtcgcatac
     1141 ggcgtagtag cgaacgacgt cgaccacgcg acgacctcgc caggccgcgg cctgcttgtc
     1201 caggcgctcg ccaccttgtc cggcagcagc tgggggttcg aaggcgacgg cggtctggcc
     1261 atgggggaaa gccgggagtt caccacggcg ccgtagccgt cgcgtgcgcc gctccaggtc
     1321 tatcagagct catgggcccc gcgcggtggc gacgtggggc cccaaccagc cggccctttg
     1381 ccatcgaccg ccagcaggcg gctaccggtg actcaccagc agttccagga agcgctcgcc
     1441 gtaacgctcg agcttggcga ccccgatccc gggcaccaac gccagctcgg cgagcgagcg
     1501 cggctccgag gccaccatct cccgcagcgt cttgtcgtgg aagacgacgt aaggcggcac
     1561 cccttgctcc ctggcgatct cgaggcgcag cgcccgcaag gcctggaaca gcgcctcctc
     1621 ggcggggccg gcggcgtcgg tggaatcggc cgaggcgaaa ctggacgagc ccggagcccc
     1681 gccagcgagt ccacgaccac gctggcgccg cgtcgaaggc ttcgcgcgct cacggtcgcg
     1741 cctcagctcg acggacacct cgccgcgcag cacggccgcc gcgcccggtc ctagcaccag
     1801 gccaccgaac ccgtcagggt cgggtagcag ctggccgttg gccaggagtt ggcgcgccac
     1861 ggagcgccaa gcgcgctcgt cgagttctgt acctatcccg aacgtgctca ggcggtcgtg
     1921 cccgaggctc tgtacgcgct cggtggcgtg cccgagcagc acgtcgatga g
//
LOCUS       DAOQYE010000408         1968 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_67781_length_1968_cov_2.433873, whole genome shotgun sequence.
ACCESSION   DAOQYE010000408 DAOQYE010000000
VERSION     DAOQYE010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1968
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67781_length_1968_cov_2.433873"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1697)
                     /locus_tag="PKN52_07175"
     CDS             complement(<1..1697)
                     /locus_tag="PKN52_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99766.1"
                     /translation="MLYTKYVVPRYTKVRVPRPRLSGAVARQASQAAVMLVAPPGYGK
                     TVLLAEVAETLARPLLWLQLDEGDNDPVAFVTALAEGARRGFPALTERLDALDLTDAG
                     GREERLLAVVVNAFADALLDDWTLVLDDLHLVANPRVLALVARLVEFPPPGMALLVAS
                     RTRPALPLNRWLARGVVKLVDVDDLRFTQPEAEAWLRRDLPGLDAAVVARLVERTEGW
                     GAGLYLAGGLLSGGTDAAALAERLSGSNPAVGGYLMDEVFERQSPELRRFLLSSAVLP
                     QFDAASCARMLGLGATESERCIELLTREQTFLQPLDADGRWYRYHPLFREFLLERLIR
                     HDGDLAARLRLAAGRAAEEQGDLASAVGFYLDAGERPEAARLLQERGTAMLAQGRGEA
                     LHRWLLRLGEELEAAPELRLLLGRVLRRRGKLHEASAVLRRVGETRAAPTAGAALTEL
                     AIIARSQGDYRRGAEWARQAVAAAGAGAAATEARAVALMELARCRCHAEGMLVGRQVA
                     EEAVEELASATQPGVDRRLRATMLGSLGQICWWQGDVAAAVGYLEEALADLDDVGGLR
                     "
BASE COUNT          295 a          715 c          702 g          256 t
ORIGIN      
        1 cgcaggccgc cgacgtcgtc taggtcggcc aacgcctcct ccaggtaccc cacggcggcg
       61 gcgacatcgc cctgccacca gcagatctgc ccgagcgagc cgagcatggt ggccctgagc
      121 cgcctgtcga cgcctggctg ggtggcggac gccaactcct cgacggcctc ctcggccacc
      181 tggcgtccca cgagcatccc ctcggcgtgg caccggcaac gtgccagttc catcagcgcg
      241 acggcgcgcg cctccgtcgc cgccgccccg gcaccggccg ccgcgaccgc ctgccgcgcc
      301 cactcggcgc cacggcggta gtcgccttgg gagcgcgcga tgatggccag ctccgtgagc
      361 gccgcgccgg cggtgggtgc ggcgcgcgtc tctcccaccc gcctcagcac cgcgctggcc
      421 tcatgcagct tgccgcgccg cctgagcacg cgtcctaaca acaggcgcaa ctccggtgcg
      481 gcttccaact cctcgcccag ccggagcagc cacctgtgta gagcctctcc gcgcccctgc
      541 gcgagcatcg cggtgccgcg ctcttggagc agcctcgccg cctcggggcg ctcaccggcg
      601 tccaggtaga agccgacggc cgaggcgagg tcgccctgct cctccgccgc ccgtccggcc
      661 gccaggcgca gccgcgcggc gaggtcaccg tcatgcctga tgagacgttc gagtaggaac
      721 tcgcggaaca gggggtggta gcggtaccag cgtccatcgg cgtcgagagg ctggaggaaa
      781 gtctgctcgc gtgtcagcag ctcgatgcac ctctcggact cggtggcacc gagcccgagc
      841 atgcgtgcgc aagaggccgc gtcgaactgc ggcaggacgg cgctggacag caggaagcgc
      901 cgcagctccg gactctgacg ctcgaatacc tcgtccatga ggtaaccgcc gacggccggg
      961 ttggagccgg aaagccgctc ggcgagggcg gccgcatcgg tgccgccgga gaggaggccg
     1021 ccggccaggt agagcccggc accccagcct tccgtgcgtt ccaccagccg ggcgacgacg
     1081 gcggcgtcaa gcccgggcag gtcgcgccgg agccaggctt ccgcctccgg ctgcgtgaag
     1141 cgcaggtcgt cgacgtcgac gagcttgacg acgccgcgcg ccagccagcg gttgagaggc
     1201 agcgccgggc gcgtgcgtga ggccaccagc agggccatgc ccggcggcgg gaactcaacg
     1261 aggcgggcca ccaaagcgag cacccgtggg ttggcgacga ggtgcagatc gtccaggacg
     1321 agggtccagt cgtcgagcaa ggcgtcggcg aacgcgttga cgaccacggc taggaggcgt
     1381 tcctcgcgtc cacccgcgtc ggtgaggtcg agcgcgtcca ggcgctcggt cagggccggg
     1441 aaaccgcgcc gcgcgccttc ggccagagcg gtcacgaagg cgacggggtc gttgtcgccc
     1501 tcgtcgagct gcaaccagag gagcggcctg gcgagcgtct cggcgacctc ggcgaggagg
     1561 accgtcttcc cgtaaccggg cggcgcgacg agcatgacgg ccgcctgtga cgcctgccgc
     1621 gcgaccgctc cggagagcct gggtcgcggc acacgcacct tcgtgtagcg tggcaccacg
     1681 tatttcgtgt agagcaccac gttcagaact gtaccagccg gctttccgcg gcctggggag
     1741 ggcctgccaa cgttctgcca acggcggaac gtagactcgc ctcgtaaccg agatagtggg
     1801 ccctgcccga ccggcatgcg catgcggcga ccggtcgtcg cgcggggcac aggagggaaa
     1861 gatgacgaga gcaccgtcgt tcgtccgctt cgcaacgctc gccctgatgc tggcgctcct
     1921 gggggtcgtg tcggcgcagt cgacgaccgc cctgagggtg gccagcac
//
LOCUS       DAOQYE010000409         1962 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_68109_length_1962_cov_2.669114, whole genome shotgun sequence.
ACCESSION   DAOQYE010000409 DAOQYE010000000
VERSION     DAOQYE010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68109_length_1962_cov_2.669114"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="PKN52_07180"
     CDS             complement(<1..452)
                     /locus_tag="PKN52_07180"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HNQ99767.1"
                     /translation="MTVLQALVLGIVQGLTEFLPVSSSGHLVLAHYFLGWGDSLPLYV
                     DIATNTGTLLAVLVFLRRDVWTALGGFARGLGSAEARREPGWRLALLIIVGSIPTAAL
                     ALLVKPVFEHLSSPLPVAIALAVTGLVLWLAPRSGPKHEPRQVTFLDAL"
     gene            complement(613..999)
                     /gene="rpsI"
                     /locus_tag="PKN52_07185"
     CDS             complement(613..999)
                     /gene="rpsI"
                     /locus_tag="PKN52_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001790547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HNQ99768.1"
                     /translation="MEQFYGTGRRKEAVARVFLRPGNGRISVNGREFQEYFRGILVGV
                     QALEPLKDTNTAGRYDAYITVKGGGPSGQADAIKLGVARALLKVDPNYRPVLKDKGHL
                     TRDARVVERKLYGLKKSRRAPQYSKR"
     gene            complement(999..1424)
                     /gene="rplM"
                     /locus_tag="PKN52_07190"
     CDS             complement(999..1424)
                     /gene="rplM"
                     /locus_tag="PKN52_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HNQ99769.1"
                     /translation="MKTYFPNELNHDWVVIDAAGQRVGRLATQIATVLKGKHKPSYTP
                     NQPGGDFVIVINADKVEFTGRKLDQKVYRRYTGYQGGLKENTAREMLEKHPVRVLEHA
                     VWGMLPKTRMGRRLIRRLKVYAGEAHPHHAQQPKKLELA"
     gene            1759..>1962
                     /locus_tag="PKN52_07195"
     CDS             1759..>1962
                     /locus_tag="PKN52_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99770.1"
                     /translation="MLLLVVVAAGVGFIYLRSSLPRVKGDVQVTGVSGPIEIVRDADA
                     VAHIYASSQADAVTQEAADAAALR"
BASE COUNT          305 a          618 c          723 g          316 t
ORIGIN      
        1 agcgcgtcga ggaacgttac ctgccgcggc tcgtgcttcg gcccggagcg cggggccagc
       61 cagagcacga ggccggtgac cgccagcgcg atggcgacgg ggagcggact gctgaggtgc
      121 tcgaacacgg gcttgacgag caaggccagc gccgccgtgg ggatggagcc gacgatgatc
      181 aagagcgcca ggcgccagcc gggctcgcgt cgcgcctcgg cggagccgag gccgcgtgcg
      241 aagccgccca gtgccgtcca gacatcgcgg cgcaggaaca ccagcacggc caggagagtg
      301 cccgtgttgg tggcgatgtc gacgtagagg gggagcgagt cgccccagcc gagaaagtag
      361 tgcgccagca ccaggtggcc ggagctcgag accggcagga actcggtcag accctggacg
      421 atgccgagga cgagcgcttg caagactgtc atccgagcac ctcgttgact tcccggtcag
      481 tcgcaggcgc cagccgtgcg cggggtcggg aaaccgggat gaagaagcga ggcgcttagg
      541 gcgcctcgcc gggggagctt cagcggtgac cggcgtcgca cccgaacggg tccgagcggc
      601 cccacgttcg actcagcgct tgctgtactg aggcgcgcgc ctggacttct tgaggccgta
      661 gagcttgcgc tccacgactc gcgcgtcgcg cgtcagatgg cccttgtcct tgaggacggg
      721 gcggtagttc gggtcgacct tgagcagggc gcgcgccacg cccagcttga tggcgtcggc
      781 ttggccgctg gggccgccac ctttgaccgt gatgtaggca tcgtaacggc cggcggtgtt
      841 ggtgtccttg agtggctcga gcgcctggac gccgacgagg atgccgcgga aatactcctg
      901 gaactcacgg ccgttgacgg agatgcggcc gttgccagga cggaggaaga cgcgtgcgac
      961 ggcctccttg cggcgtccgg taccgtagaa ctgttccatc aagccaactc caacttcttg
     1021 ggctgctgcg catggtgcgg gtgggcctca ccggcgtaga ccttgaggcg gcggatgagg
     1081 cggcgcccca tgcgggtctt ggggagcatg ccccagacgg cgtgctcgag cacgcgcaca
     1141 gggtgcttct ccagcatctc gcgcgccgtg ttctccttga ggccgccctg gtagcccgtg
     1201 tagcggcggt acaccttctg atccagcttg cggccggtga actccacctt gtccgcgttg
     1261 atcacgatga cgaagtcgcc gcccggctgg ttgggcgtgt agctgggctt gtgcttgccc
     1321 ttcaacacgg tggcgatctg ggtagcgagc ctgcccacgc gctgaccggc cgcgtctatc
     1381 acgacccagt cgtggttgag ctcgttggga aagtacgtct tcacgttggt cacgtccttg
     1441 cgggctttgc cggtttgatc ggggcggcaa aacttgaagc caaaagtgag tctagcacgg
     1501 tggcgcggcg cgtgtatagg ccggtggggt cggcgcgccg cctccgaccc caccgccggc
     1561 gccggcgccg cgccggccgt ggtcaggcgg ccaagagcta tgccagggcc aagcggcaaa
     1621 gggccgggcc aggaccaggc gaccaagggc taagatgccc ccaaggaaga gggttctaag
     1681 tcggcgcgcc gggccgggct cttggtcgcg gcgtaacggg agttgtccat gagaacgctt
     1741 ggtcggatac tcggcatctt gctgcttctg gtcgtcgtcg ccgccggcgt cggcttcatc
     1801 tacctccgct cgtcgctgcc ccgcgtcaag ggtgacgtcc aggtgacggg ggtgagcggc
     1861 cccatcgaga tcgtcaggga cgcggacgcc gtcgctcaca tctacgcgtc gagccaggcg
     1921 gacgcggtca cgcaggaagc ggcggacgcg gcggcgctgc gc
//
LOCUS       DAOQYE010000410         1962 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_68110_length_1962_cov_2.658626, whole genome shotgun sequence.
ACCESSION   DAOQYE010000410 DAOQYE010000000
VERSION     DAOQYE010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68110_length_1962_cov_2.658626"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            294..1157
                     /locus_tag="PKN52_07200"
     CDS             294..1157
                     /locus_tag="PKN52_07200"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HNQ99771.1"
                     /translation="MATKAGTLDLLSSAPGIELVRGTLKRGERLNFVPSRPGEAAAHE
                     VCPLTDGVLLAPDTLGAQRLSAGSLITSEGLQEPVSFTAETFVRFVYITSAPMFHHIS
                     RDMTELRRLAVEIEVRDGYTADHCERLQRLSYATGLELGLNPSQLYALDFGSYLHDVG
                     KVRVPVSILTKPSALDAEEWAIIRRHPTFGREMLEPTFMRAASPIVEQHHERSDGSGY
                     PFGLTENEILVESAIVAVADTYDAMTSDRPYRRGRSSAEALAELASLAGTQHPKEVVR
                     AFQAAISQMQE"
     gene            1230..1535
                     /locus_tag="PKN52_07205"
     CDS             1230..1535
                     /locus_tag="PKN52_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99772.1"
                     /translation="MRTEKEQTLRNRLFTVLVATLALASVGGAFAADWHLTANLPLVT
                     AILDELPTAGIGGPLHDMQEGVHAAVTKLTGVGVPHDYLWLHVGKTSIPVDPITFSK"
     gene            complement(1631..>1962)
                     /locus_tag="PKN52_07210"
     CDS             complement(1631..>1962)
                     /locus_tag="PKN52_07210"
                     /inference="COORDINATES: protein motif:HMM:NF015534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HNQ99773.1"
                     /translation="RQSGLADLLPSLDAVYVGLSAGSMVMAPRIGREFVGWTPPTGGD
                     ETLGLVDLAIFPHLDHPMLPSNTLAAAERWAAGVRVPAYAIDDETAIRVVDGAVDVVS
                     EGNWKLF"
BASE COUNT          331 a          702 c          620 g          309 t
ORIGIN      
        1 tcctctcccg taagcggctt gccggcaccc aggtgccgac gcacgcgaac gttgaggaag
       61 aagagccctg ccatgacgaa gcgaactgta gcggaagggt ctggagttgt caagcgcaag
      121 cacggctccg gccacggcgc ccgcttacgg agaaggaccg acctgtaagg ctcccgtcat
      181 gccccgcgtc gcaacctgaa gcccttgtgg gccactcatc cacggttgcc accccgatag
      241 cctcgttccg gacgggcctg agcatcgtcc cggccggggc gagcctggag tcggtggcca
      301 ccaaggccgg caccctcgac ctactctcgt cggctccagg catcgagctg gtgcgcggca
      361 ccttgaagcg cggcgagcgc ctcaacttcg tgccgtcaag acccggcgag gccgcggccc
      421 acgaggtatg tccgctcacg gacggcgtcc tcttggctcc cgatacgctg ggggcccagc
      481 gcctgtccgc cggcagcctc atcacgtccg aagggctgca ggagcccgtg agtttcacgg
      541 cggagacctt cgtgcgcttc gtctacatca cctcggctcc catgttccac cacatcagcc
      601 gcgacatgac cgagttgcgg cggctggccg tcgagatcga ggtgagggac ggctacaccg
      661 ccgaccactg cgagcggttg cagcgcctgt cgtacgccac cggcctcgag ctggggttga
      721 acccgtccca gctctatgcc ctcgacttcg gctcgtacct ccacgacgtc ggcaaggtgc
      781 gcgtgcccgt cagcatcctc acgaagccga gcgccctgga cgcggaggag tgggcgatca
      841 tcaggcggca ccctacgttc ggccgcgaga tgctcgagcc gaccttcatg cgcgcggcca
      901 gcccgatcgt cgagcagcac cacgaacgct ccgacggcag cggctacccc ttcggcctga
      961 ccgagaacga gatcctcgtc gaatccgcca tcgttgccgt cgcagacacc tatgacgcca
     1021 tgacctccga caggccgtat cggcgtggcc gctcgtcggc cgaggccctc gccgagctcg
     1081 cttcgctggc cggcacccaa caccccaagg aggtcgtccg cgctttccag gcagcgatca
     1141 gccagatgca ggagtagacc ggagcctttc agcccagctt tcgcatccca ggtcggcagc
     1201 cgggctgcca accagagaag gagaagacct tgagaacaga gaaggagcag accctgagaa
     1261 accgtctgtt caccgtactc gtcgccacgt tggcgctggc ttcagttggc ggcgccttcg
     1321 cggccgactg gcacctcacc gcgaacctgc ccctggtaac ggccatcctc gacgagttgc
     1381 ctacggccgg catcggcggg ccgctgcacg atatgcagga gggcgtccat gccgccgtga
     1441 ccaagctgac cggcgtagga gtgccgcacg attacctgtg gctgcatgtc ggcaagacgt
     1501 ccataccagt tgatcccatc acgttcagca agtaactgaa cgccggtcgg tcaggatcga
     1561 ccggcccgag tagcgcgcgg cggggccagg cggatatccg cctggccccg ctccctgctc
     1621 gcgtcggctc ctagaagagc ttccagttcc cttcgctgac tacgtcgacc gctccatcga
     1681 ccaccctgat cgccgtctcg tcgtcgatgg cgtacgccgg cacccgcacg cctgcggccc
     1741 agcgttccgc cgcggccagg gtgttcgacg ggagcatcgg gtggtcgagg tgggggaaga
     1801 tggcgaggtc gaccaggccg agcgtctcgt cgccgccggt gggcggcgtc cagcccacga
     1861 actccctgcc gatccttggg gccatcacca tgctgccggc gctcaggcct acgtagaccg
     1921 cgtcgagtga cggcaggagg tccgccaaac cgctctgccg ca
//
LOCUS       DAOQYE010000411         1962 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_68118_length_1962_cov_2.170949, whole genome shotgun sequence.
ACCESSION   DAOQYE010000411 DAOQYE010000000
VERSION     DAOQYE010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68118_length_1962_cov_2.170949"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1929)
                     /locus_tag="PKN52_07215"
     CDS             complement(<1..1929)
                     /locus_tag="PKN52_07215"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HNQ99774.1"
                     /translation="MALQNAPRPAKRAPLAAPSDPTVAAELERRLRASVRGEVRFDAR
                     ARALYATDASPYRMAPVGIVVPLDEADVKAAVKAAHELKVPVLPRGGGTSLAGQTVAA
                     ALVIDFSKHLTGLLELNVSERWVRVQPGLVRDQLNAKLAQHRLQFTPDVATTDRANVG
                     GMVANNSAGTRSIKYGKSVDQVIEARVLLVDGTELELKPLTGADLTAKLAEAGREGEL
                     YRAVHTLVTEHAAEIAERYPKVMRRVGGYNLDELTNAGAHGQRPFNLAKLVSGSEGTL
                     ALILELKLALHPVPKRRIVTMLHFASLTAAFEAVKFVNRHGPSAVEIIDRALIVLGRE
                     NPAMAPLMGWVVGDPEAVLLVEFDGEDLGACRRALAGLEADQDVAALSYGAYLAEDPK
                     QQKEISDFRRDGLGIYATMPGAAKPTPFIEDAAIPVEHLAQYIPEVIAVCERHGTRAV
                     FYAHASVGVIHVRPLLDLKDAAGIEKFRAISEEVFDLVVKYGGSWSGEHGDGLIRSYQ
                     NRRLFGDELYEAFIAVKRAFDPDWLLNPGKIVEAPAMTENLRYGADYPTLELPTVFDF
                     SEQDGFLGAVEACTGVGACRKVGAGTMCPSYMGTRDEDNSTRGRANVLREALTGGLQG
                     GPTSDDVYRVLDLCLECKA"
BASE COUNT          259 a          662 c          725 g          316 t
ORIGIN      
        1 cgccttgcac tcgaggcaga ggtccagcac ccggtagacg tcgtcgctcg tcggtccgcc
       61 ctgcaggccg ccggtgagcg cctcgcggag cacgttcgcg cgtccgcgcg tggagttgtc
      121 ctcgtcgcgc gtgcccatgt aggacgggca catggtcccg gcgcctacct tgcgacacgc
      181 gcctaccccc gtgcaggcct cgacggcgcc caggaagccg tcctgttcgg agaagtcgaa
      241 cacggtcggc agttctaggg tcgggtagtc ggcgccgtag cgcaggttct ccgtcatggc
      301 cggcgcctcg acgatcttgc cggggttcag gagccagtcc gggtcgaacg cgcgcttcac
      361 cgcgatgaac gcctcgtaca gctcgtcgcc gaacaggcgg cggttctggt aggagcggat
      421 gagcccgtcg ccgtgctcgc cgctccacga gccgccgtac ttcacgacga ggtcgaagac
      481 ctcctcgctg atggcccgga acttctcgat gccggcggcg tccttgaggt cgagcagcgg
      541 cctgacgtgg atgacgccga cggacgcgtg cgcgtagaac acggccctgg tgccgtggcg
      601 ctcgcagacc gcgatgacct ccgggatgta ctgcgcgagg tgctcgacgg ggatggcggc
      661 gtcctcgatg aacggcgtcg gcttggccgc acccggcatc gtggcgtaga tgccgaggcc
      721 gtcgcgccgg aagtccgaga tctccttctg ctgcttcggg tcctcggcca ggtaggcgcc
      781 ataggacagc gcggcgacgt cctggtcggc ttccagaccg gcgagggcgc ggcggcacgc
      841 tccaaggtcc tcgccgtcga actccacgag gagcacggcc tccgggtcgc ccaccaccca
      901 gcccatcagc ggggccatcg ccgggttctc gcgccctagg acgatgaggg cgcggtcgat
      961 gatctcgacg gccgaagggc catgccggtt cacgaacttc actgcctcga acgccgccgt
     1021 gaggctggcg aagtggagca tggtcactat ccggcgcttg ggcacggggt gcagcgccag
     1081 cttgagctcg aggatgaggg ccagcgtgcc ctcgctgccg gacacgagct tggcgaggtt
     1141 gaaggggcgt tggccgtggg cgccggcgtt cgtcagctcg tccaggttgt agccgccgac
     1201 gcggcgcatg accttcgggt agcgctcggc gatctcggcg gcgtgctcgg tgacgagcgt
     1261 atggacggcg cggtatagct cgccttcgcg gcccgcctcc gcgagcttgg ccgtcaggtc
     1321 cgcgcccgtc agcggcttca gctcgagctc cgtgccgtcc acgaggagga ccctggcctc
     1381 gatgacctgg tcgacgctct tgccgtactt gatggagcgc gtgcccgccg agttgttggc
     1441 caccatgccg cccacgttgg cgcggtcggt ggttgccacg tcgggcgtga actgcaagcg
     1501 gtgctgtgcc agcttggcgt tgagctggtc gcgcaccagg ccgggctgca cgcgcaccca
     1561 gcgctcggac acgttgagtt ccagcagccc agtgaggtgc ttggagaagt cgatcaccag
     1621 cgcggcggcc acggtctggc cggccaggct ggtgccgccg ccgcgcggga ggacggggac
     1681 cttgagctcg tgcgcggcct tgaccgctgc cttcacgtcg gcctcgtcga gcggcaccac
     1741 gatccccacg ggggccatgc ggtaggggct ggcgtcggtg gcgtagaggg cgcgggcgcg
     1801 ggcgtcgaag cgcacctcgc cgcgcacgct cgcgcgcagg cggcgctcca gttcggcggc
     1861 tacggtcggg tcggaggggg ccgccaacgg ggcccgcttg gccgggcgag gtgcgttctg
     1921 gagtgccacg cgctcagtat agggcgtgtg gcacgcgctc cc
//
LOCUS       DAOQYE010000412         1959 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_68271_length_1959_cov_3.055147, whole genome shotgun sequence.
ACCESSION   DAOQYE010000412 DAOQYE010000000
VERSION     DAOQYE010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1959
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68271_length_1959_cov_3.055147"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..950
                     /locus_tag="PKN52_07220"
     CDS             <1..950
                     /locus_tag="PKN52_07220"
                     /inference="COORDINATES: protein motif:HMM:NF016994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="erythromycin esterase family protein"
                     /protein_id="HNQ99775.1"
                     /translation="YGLESAWQETRTEADVPSLAKHVYGFWRDGPHVSPLWDLVRQRL
                     DSERPLIVTGIDVRHSGGYPKADVAVALETHLARHGVTLDPDWQRFRALLDDLLQKEY
                     GHRVDANDRMRFMEGLVSLRDQVTGSDVGSSFWQQELRNLSWTARNAWGFEGRDEGMG
                     RNLVWLATERYPGKKIVVWAHNFHIVRSAEALGAHHPAYARALEKYPDTPLGEVAVRE
                     LGDAVRSISLVAGRGWHSPYAWRGDTATREELSPPPSYSLEAAALDRDMPCGYLDLTS
                     TTQSFAMNGTEHAALVTAPWGRIFDGAIYIRDMTGLGEK"
     gene            998..1231
                     /locus_tag="PKN52_07225"
     CDS             998..1231
                     /locus_tag="PKN52_07225"
                     /inference="COORDINATES: protein motif:HMM:NF027267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HNQ99776.1"
                     /translation="MCWDVLIVTSRLTSKGQTTIPRSVRQGLQLREGDVLEFELMDGY
                     VILRKAQPSHSEDPFVAFEEWAGDADQRAYGDL"
     gene            complement(1637..>1959)
                     /locus_tag="PKN52_07230"
     CDS             complement(1637..>1959)
                     /locus_tag="PKN52_07230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99777.1"
                     /translation="EDTEAFRMTADELKTRLLEARSSPAAATAETWEMAQVMLAPLVI
                     GHPDPELRDQLVYTTLCDWIMSGVLRPDQLRAVTTTVMDDMHLFYRLGARRRSGRRNA
                     HTRQ"
BASE COUNT          387 a          597 c          607 g          368 t
ORIGIN      
        1 tctacggttt ggaaagtgcc tggcaggaga cacgcacaga agcggacgtt ccttctctgg
       61 caaagcacgt atacggtttc tggcgggatg gcccgcacgt ctcgccgctg tgggacctag
      121 tgcgtcaacg tctcgatagc gagcggcctc taatcgttac gggcatcgac gtccgccatt
      181 ctggaggata ccccaaggca gacgtcgctg tggcgctaga aacccatctc gctagacacg
      241 gcgtgacgct ggatccggac tggcagcggt tccgtgcgct cctagacgat ctgctccaga
      301 aggagtacgg ccatcgagta gacgcgaacg accggatgcg gttcatggaa ggcctggtga
      361 gcctccgcga tcaggtcacc ggttccgatg tcggtagttc cttctggcag caggaactgc
      421 gcaacctctc ttggacagca cgcaacgcat ggggcttcga agggcgcgac gaaggcatgg
      481 gccgcaacct tgtctggttg gcaaccgagc gctatcccgg caagaagatc gtcgtatggg
      541 cccacaactt ccacatcgtc cggagtgccg aggctctcgg ggcccatcat cctgcctacg
      601 cgcgcgcact cgagaagtac cccgacactc cgcttggtga agtcgccgtt cgcgagctgg
      661 gagacgcagt acggtccatc tctctagtcg caggtcgcgg ttggcactcg ccatacgcct
      721 ggagaggcga caccgcaact cgcgaagagc tttccccacc gccttcttac tcattggaag
      781 ctgccgccct cgaccgagac atgccgtgcg ggtacctgga tctcacgagc acgacacagt
      841 cgttcgcgat gaacggaacc gagcatgctg cgctggtcac cgctccgtgg ggccgcatct
      901 tcgacggagc catctacatc cgggacatga caggtcttgg tgagaagtga agaacgtagg
      961 cgatcgactg taggagtcgg caaggaagtt ccttacaatg tgctgggatg tcctcatagt
     1021 caccagtcgc ctcaccagca aggggcagac aaccatcccg cgaagcgtgc gccaagggct
     1081 acaactccgt gaaggcgacg tactcgagtt cgagctgatg gacggatacg tgatcctccg
     1141 caaggcccaa ccgtcacaca gcgaggatcc gttcgtggca ttcgaggagt gggccggtga
     1201 cgccgaccag cgggcctatg gcgatctttg accaaggtga cgtcatacgc gttctgttcc
     1261 ctaccgatag gcccgtccgt cagcaccgtc ctgccctagt gctctcccgg acaggcgtcg
     1321 acgagggcca gaggctctta tgggtgctga tgatcactag cgccgagaac cggcgatggc
     1381 ccggcgacgt ggatgtaggc gagcggtatg aagacgcggg cctgcccgca ccgtcattgg
     1441 tcaggacggc gaagatcgct acgatcgagg gcatacgagt agatcggatc ggtcgtctgc
     1501 ccagagagct cctcgagatg gtgttcacaa ccgtagccac gacgctccgg cagtagtgcg
     1561 gcagtttcta tcgcgggtcc ggcagaccca ccagcccttg agcgttagtc atagcttaga
     1621 gaactcgaac agcaactcat tgacgcgtat gcgcgttgcg gcggcccgag cgacggcgtg
     1681 cacccagccg atagaaaagg tgcatgtcgt ccatcacggt agtcgtgacc gctcgcaatt
     1741 ggtccggtcg cagcactccg ctcatgatcc agtcgcatag ggtcgtgtag acgagctggt
     1801 cgcggagttc aggatcaggg tggccgatga ccagcggcgc cagcatgacc tgggccatct
     1861 cccacgtttc cgctgtcgca gcagcaggcg aactgcgcgc ttccagaagt cttgtcttga
     1921 gctcgtctgc cgtcattcgg aacgcctcgg tgtcttccc
//
LOCUS       DAOQYE010000413         1957 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_68380_length_1957_cov_3.172450, whole genome shotgun sequence.
ACCESSION   DAOQYE010000413 DAOQYE010000000
VERSION     DAOQYE010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1957
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68380_length_1957_cov_3.172450"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..675)
                     /locus_tag="PKN52_07235"
     CDS             complement(<1..675)
                     /locus_tag="PKN52_07235"
                     /inference="COORDINATES: protein motif:HMM:NF021445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HNQ99778.1"
                     /translation="MTALDQTEARVAQVGGRTPARGGDHAAARELILRHGWNTTVYQV
                     LNPDIRHWFTRDGEALVGYVQHAGVRVVAGAPVCDYARLPEVAQEFERDARAAGLTTC
                     YFAVEPRWLELVRRRSEVALIGAQPVWDAATWREAVDAHASVRAQFNRARNKGVGVER
                     WPLARLVAGQPGAAELRGLRERWAAAHGLPPLGFLTGVELLPKDAEPDGPLTLDRRLY
                     VASRLGA"
     gene            889..1362
                     /gene="greA"
                     /locus_tag="PKN52_07240"
     CDS             889..1362
                     /gene="greA"
                     /locus_tag="PKN52_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003711 - transcription elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HNQ99779.1"
                     /translation="MSKEPIQMTARGLEKLKEELRYLKEEKRQELADYMGAALADGDL
                     RESAAYDEARLLQSANEARIADLEELVHRAVVVEHDDGEAVARLGASLTLQSQDGENV
                     VFHLVGTHEADILEGRVSDESPLGRSLVGRRVGDNVTVQMPVGAAVYRVVAVTFD"
     gene            1443..>1957
                     /locus_tag="PKN52_07245"
     CDS             1443..>1957
                     /locus_tag="PKN52_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99780.1"
                     /translation="MVRWAALAFAVVVAALVALALSPQTPPSVPSAVIELSSVNLTLF
                     PQSDPDAVWYFAAPTASYAPDRQETTLDEVSDGRRTLGGETDFTLTADSVTIDRADNL
                     RGDHMHAHLVADDLDLDMLAKGSRTVFIDQRAGRFEVPRAVMTGKDLGESVFEDMRIS
                     FDFTEFEAGGPG"
BASE COUNT          301 a          704 c          666 g          286 t
ORIGIN      
        1 ggcgcccaga cgcgacgcca catagaggcg ccggtccagc gtgaggggtc cgtccggttc
       61 ggcgtccttg ggcagcagct cgacaccggt gaggaacccg agcggcggca acccgtgggc
      121 ggccgcccac cgctcgcgca gtccgcgcaa ctcggcggca cccggttgac ccgctacgag
      181 gcgcgccagc ggccagcgct ctacccccac ccccttgttg cgcgcgcggt tgaactgcgc
      241 ccgcacagac gcgtgcgcgt cgaccgcctc gcgccaggtc gcggcgtccc aaacgggctg
      301 cgcaccgatg agcgctacct ctgatcggcg cctcacgagt tccagccacc gcggctccac
      361 cgcgaagtag caggttgtga ggccggcggc tctagcgtcc cgctcgaact cctgcgcgac
      421 ctcgggcagc cgggcgtagt cgcagacggg agctccggcc accaccctca cgccggcatg
      481 ctggacgtag ccgacgagcg cttcgccgtc gcgcgtgaac cagtgccgga tgtcggggtt
      541 gagtacctgg tagaccgtgg tgttccagcc gtggcggagg atcagctcac gcgcggcagc
      601 gtggtcaccg ccccgtgcag gagtgcggcc accgacttgc gcgacccgcg cttccgtctg
      661 gtccagggcc gtcacgagcg catggtagcg ccgatcaacg gacaccggcc gtgaacgcgg
      721 gtccaccgga agtggcgccg ccccctgact tgcacgcgtg agtccggcat ataatcccca
      781 ccggacgttg cccggcggtc ccattgcttc cgccgactcg cttgccgcca agcccgccca
      841 gggcggcgtt cgcgggcggg agcctacccc cgccaggtga ggagcacagt gtccaaggaa
      901 cccatccaga tgaccgcgcg cgggctggag aagctcaagg aagagcttcg ctacctgaag
      961 gaagagaagc ggcaggagct cgccgactac atgggcgccg cgctggccga cggcgacttg
     1021 cgcgagagcg ccgcttacga cgaagcgcgg ctgctgcaga gcgccaacga ggcgcgcatc
     1081 gccgacctcg aggagctcgt gcaccgcgcc gtggtggtcg agcacgacga cggcgaggcc
     1141 gtggcgcgcc tcggcgcctc cctgaccctg cagtcgcagg acggcgagaa cgtcgtgttc
     1201 cacctcgtgg gcacgcacga ggccgacatc ctcgaagggc gcgtcagcga cgagtcgccc
     1261 ttggggcgca gcctcgtcgg acgccgggtc ggcgacaacg tcaccgtgca gatgccggtc
     1321 ggcgccgccg tctatcgcgt ggtggcggtc accttcgact gaccggcccg taaagcgtag
     1381 ctaagccttc ggttatcgcc gcggtcacct ttctcagccc cacgcgttgg tagtctcgct
     1441 gcatggtgcg ctgggcagcg ctcgctttcg ccgtcgtggt ggcggccctc gtcgctctgg
     1501 cgctgtctcc gcagacaccg ccgagcgtcc cgagcgcggt catcgagttg agttcggtga
     1561 acctcacgtt gttcccgcag tcggacccgg acgccgtctg gtacttcgcc gcgccgacgg
     1621 cgtcttacgc tcccgatcgc caggagacga ccctggacga ggtatcggac gggcgccgca
     1681 cgcttggggg ggagacggac ttcacgctga cggccgacag cgtcacgatc gaccgcgccg
     1741 acaacctgcg cggtgaccat atgcacgcgc acctcgtcgc ggacgacctc gacctcgaca
     1801 tgctcgccaa aggctctcgc accgtgttca tcgaccaacg cgccggacgc ttcgaggtcc
     1861 cacgcgcggt catgaccggc aaggacctcg gcgagagcgt cttcgaggac atgcgcatca
     1921 gtttcgactt cactgagttc gaggcgggcg gcccggg
//
LOCUS       DAOQYE010000414         1956 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_68491_length_1956_cov_1.991057, whole genome shotgun sequence.
ACCESSION   DAOQYE010000414 DAOQYE010000000
VERSION     DAOQYE010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1956
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68491_length_1956_cov_1.991057"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="PKN52_07250"
     CDS             complement(<1..203)
                     /locus_tag="PKN52_07250"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HNQ99781.1"
                     /translation="MKTKAETIDEYLADVDPERRAELERLRALVKRIVPAVQETISYR
                     MPTLLYRGRPLVYFTASKKHMSF"
     gene            391..1032
                     /locus_tag="PKN52_07255"
     CDS             391..1032
                     /locus_tag="PKN52_07255"
                     /inference="COORDINATES: protein motif:HMM:NF012696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HNQ99782.1"
                     /translation="MADPADLLSRNRVWAADMVASDPDFFARLESQQAPRYLWIGCSD
                     SRVSANVIAGLEPGEVFVHRNVGNVMANADLNALTVLDFAVSVLNVEHVIVVGHYGCG
                     GVSAALDGKSHGLLDNWLRHIKDVAAKHATLLDSLPTEARARRLAELNAIEQARNVCH
                     TSIVQEAWAAGHPLTVRAWIYALHDGVLHDLRFIVRGPGDLQPAYRLSLGEAG"
     gene            1414..>1956
                     /locus_tag="PKN52_07260"
     CDS             1414..>1956
                     /locus_tag="PKN52_07260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HNQ99783.1"
                     /translation="MTDASPPPDPHATPTAASAEAIGRNLDSVRDRIAAACRRVGRDP
                     GEVRLLPVSKTVPEERIRMAYAAGCRYLGENKVQEAMRKHDALPDLDDLKWSIIGHLQ
                     TNKAKHVARFASEFQALDSLRVAEALDRRLEAEGRTLDVFVQVNSSDEESKFGLAPAD
                     VPAFVRELPNYPRLRVLGLMT"
BASE COUNT          312 a          693 c          670 g          281 t
ORIGIN      
        1 tagaagctca tgtgcttctt ggaggcggtg aagtaaacga ggggccggcc ccggtacaga
       61 agggtcggca tcctgtagct gatcgtctcc tggacggctg ggacgatccg cttgaccagg
      121 gcgcggagcc gctcgagctc cgctcgacgc tccgggtcaa catcggccag gtattcgtcg
      181 atggtctccg ccttcgtctt catggccaca tgctagcaac ggctcggtcc gccgaccgcg
      241 cgggtggggt ggggcagcaa gccgaccgcg cggtgcaccc gcaggcacgg tcacgcggac
      301 cgccggcggg gcgccgcgcg gtcggtcgcc tcacccgcgg acggcccgtg tcacaccgac
      361 tgcctccgcg gccgctatag actcgcccac gtggcagacc cggcagacct cctctcacgc
      421 aaccgcgtct gggcagccga catggtcgcg agcgacccgg acttcttcgc gcgcctcgag
      481 tcccagcagg caccccgcta cctctggata ggctgctccg actcgcgcgt gagcgcgaac
      541 gtcatcgcag gtctcgagcc gggcgaggtg ttcgtccacc gcaacgtcgg caacgtcatg
      601 gcgaacgcgg acctcaacgc gctcactgtc ctcgacttcg ccgtctccgt tctgaacgtg
      661 gagcacgtga tagtcgtcgg gcattacggc tgcggcggcg tttccgccgc gctcgacggc
      721 aagagccatg ggctactcga caactggctg cggcacatca aggacgtggc tgccaagcac
      781 gccacgctcc tcgactccct cccgacagag gcccgggcca ggcgtctcgc ggagctgaac
      841 gccatcgagc aggcgcgcaa cgtctgccac acgagcatcg tccaggaggc gtgggccgcc
      901 gggcatccgc tcaccgtgcg cgcgtggatc tacgcgctgc atgacggcgt gctgcacgac
      961 ctccgcttca tcgtgcgtgg gcccggggac ctgcagcccg cgtaccggct gtcgctcggg
     1021 gaggccggct agggctaggc gggcgggcgc tcggcgggcg ctcggcgggc gatgccttgg
     1081 tcgagcaggt ggtcggcgga cagctcgagc gggcggccag gttgaccggt tgaggggagc
     1141 gcccgagagg cgctcggtgg gcgggcgagg aggtagtgtc ctcagtagag ggatatgcaa
     1201 cagccaggtt gcatatgggt ccggttcgcg aacaacagac caccatgcgc agccgccgcg
     1261 aacgggccca tcatccaacg acggcagacg atgatcgcgt tggcagcgga gcagtcagca
     1321 agataccgac cggtctagct ggcccgccga ggtggtcgcg cgatcaactt cgccactagc
     1381 ccctcaccgc tcgccgcggg ctatcctgcg cacgtgacag acgcgagccc tcctccagac
     1441 ccccacgcca ccccgaccgc cgcatccgca gaggcgatcg ggcgcaacct cgactccgtg
     1501 cgcgaccgga tcgccgccgc ctgccgccgc gtaggccgcg acccgggcga ggtgcggctc
     1561 ctgcccgtca gcaagacggt gccggaggag cgcatccgca tggcgtacgc ggccggctgc
     1621 cgttacctgg gcgagaacaa ggtgcaggag gccatgcgca agcacgacgc gctcccagat
     1681 ctcgacgacc tcaagtggtc catcatcggc cacctgcaga cgaacaaggc caagcacgtc
     1741 gcgcgcttcg ccagcgagtt ccaggcgctc gacagcctgc gggtcgccga ggcgctcgac
     1801 cggcgcctcg aggccgaggg gcgcacgctc gacgtcttcg tgcaggtcaa cagctccgac
     1861 gaggagagca agttcggcct tgcgccggcc gacgtgcccg ctttcgtgcg cgagctgccc
     1921 aactacccgc gcctgcgcgt gctcgggctg atgacc
//
LOCUS       DAOQYE010000415         1953 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_68658_length_1953_cov_2.250263, whole genome shotgun sequence.
ACCESSION   DAOQYE010000415 DAOQYE010000000
VERSION     DAOQYE010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1953
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68658_length_1953_cov_2.250263"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="PKN52_07265"
     CDS             complement(<1..267)
                     /locus_tag="PKN52_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNQ99784.1"
                     /translation="MNGHENGNGSDYTADSIKVLRGLEGVRKRPAMYVQGGTGVDGYH
                     QLLTEIIDNAIDEFLAGYADTVEVTLNADGSATVTDNGRGLPVDM"
     gene            complement(681..1901)
                     /locus_tag="PKN52_07270"
     CDS             complement(681..1901)
                     /locus_tag="PKN52_07270"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HNQ99785.1"
                     /translation="MLARARAASRALPTAERRGALAAIGAALLIDAPAILTANAADVA
                     AERERGTSAALVDRLRLSPERIAAISHAVEQIADLPDPVGKVLEGWELPNGLRVRRVS
                     VPFGVIGMVYESRPNVTVDAAALALKAGSAAVLRGSSNALASNRAIVASMRSALARAG
                     LPEDAVQLIDSSDRELVTELLRARGKVDLVIPRGGAGLIRHVVDTASVPVIETGVGNC
                     HLYVDAGADLAMAERLAMNAKVQRPGVCNAIETLLVHARVAGVFLPSIATELRAAGVR
                     LYGCARARAIVGDMEAAGEREWETEFLDLELAVRVVDDLDAALDHIARYGTQHTEAIV
                     TQDGTAAERFRREVDAAVVVVNASTRFTDGFEFGFGAEIGISTQKLHARGPLGLEQIV
                     TYKYLVDGVGQTRG"
BASE COUNT          287 a          687 c          660 g          319 t
ORIGIN      
        1 catgtcgaca gggaggccgc gaccgttgtc ggtgacggtg gccgagccgt ccgcgttcag
       61 cgtcacctcg accgtgtcgg cgtatccggc caggaactcg tcgatggcgt tgtcgatgat
      121 ctcggtgagg agctggtggt agccgtccac cccggtgcca ccttgcacgt acatggcggg
      181 gcgcttgcgc acgccctcga gaccgcgcag gaccttgatg ctgtcggcag tgtaatcgct
      241 gccgttaccg ttctcgtgac cgttcaacta gacctcccgg gcgcccgcct gccccgcaca
      301 cttgctaccg cactcgccac ggcgcagtcg ttgcgacgcg ctcgttgcga cgcgctcgct
      361 accgcccgcc ggctgccgcc tcgccgttgc aacctcgctg ttgcgaggcc ctcgctgatg
      421 cgagactctc gctcgtgagc gactctcgct cttcagcgac tctcgctcgt gcgagaccct
      481 agctcgctag gaccccttga tggcgccaga cgaccgcgga cgttgtcgac gtgatggagt
      541 ggagacgagc gcggcgagct gcgtagcagg cactggccca ctaacagccc tattctatcc
      601 gcataacggt aggctggtca aacgttctac gacgtcctgg acggtatttc tgcctagtca
      661 agatgggcag tgagcgcccc tcagccgcgg gtctgaccga caccgtcaac gaggtacttg
      721 tacgtcacga tctgctcgag tccgagcggc cccctcgcat ggagtttctg cgtgctgatg
      781 ccgatctcgg cgccgaaccc gaactcgaag ccgtccgtga agcgcgtgga ggcgttcacc
      841 acgacgacgg ccgcgtccac ctcgcgccgg aaccgttcgg cggccgtccc gtcctgcgtc
      901 acgatcgctt ccgtgtgctg cgtcccgtac cgggcgatgt ggtcaagggc cgcgtcgagg
      961 tcatcgacca cgcgcaccgc cagctcgagg tcgaggaact cggtctccca ctcgcgctca
     1021 cccgcggcct ccatgtcgcc gacgatggcc cgggccctcg cgcaaccgta gagcctgacg
     1081 ccggccgcac gcagctccgt ggcgatgctt gggaggaaca ctccggcgac ccgcgcatgc
     1141 acgagcagcg tctcgatggc gttacagacg ccgggtcgct gcaccttggc gttcatggcg
     1201 aggcgttcgg ccatggcgag gtcggcgcca gcgtcgacgt agaggtggca gttgccgaca
     1261 cccgtctcga tcaccggcac gctggccgtg tcgaccacgt ggcggataag cccggcgccg
     1321 ccgcgcggga tgacgaggtc gaccttaccc ctggctcgca gaagttccgt gacgagctcg
     1381 cgatcggagg agtcgatcag ttggacggcg tcctccggca gcccggctcg ggcgagagcg
     1441 gaacgcatgc tggcgacgat cgccctgttg ctcgccagcg cgttggagga gccccgcagc
     1501 acggcggcgc tgccggcctt gagggccaga gcggccgcat cgacggtcac gttgggccgc
     1561 gactcgtaga ccatcccgat caccccgaac ggcacgctca cgcgccgtac ccgcagaccg
     1621 ttcggcaact cccatccctc caagaccttg cccacggggt cgggcaagtc ggcgatctgc
     1681 tcgaccgcat gcgagatggc cgcgatgcgc tccggcgaca gtctcagccg atcgacgagg
     1741 gcggcgctcg tgccccgctc gcgctcggcg gccacgtcgg ccgcgttggc ggtcaggatg
     1801 gccggtgcgt cgatcagcag ggccgcgccg atggccgcga gcgcgccgcg gcgctcggcc
     1861 gtcggtaggg cgcgagaggc ggccctcgcc ctggcaagca gggcgtcgag gtaaccggca
     1921 tcgtgagggc cggtgggggt cgggaccgac gct
//
LOCUS       DAOQYE010000416         1949 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_68875_length_1949_cov_2.657339, whole genome shotgun sequence.
ACCESSION   DAOQYE010000416 DAOQYE010000000
VERSION     DAOQYE010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68875_length_1949_cov_2.657339"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="PKN52_07275"
     CDS             complement(<1..211)
                     /locus_tag="PKN52_07275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99786.1"
                     /translation="MTLQDYANQVYQKHSLASEKVKSFVSKTEVAGKPNQRRLSVVLA
                     DRRNEILIVEKDSEICEALGPHVTQH"
     gene            233..499
                     /locus_tag="PKN52_07280"
     CDS             233..499
                     /locus_tag="PKN52_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ99787.1"
                     /translation="MPDGDAEVGVVALVEVIGVGFGLLLANVITNGAGALTGFVLQPR
                     LPWYLALLALLASPVVGLVASLFPARRAARMAPTKAFASWSEHV"
     gene            492..1229
                     /locus_tag="PKN52_07285"
     CDS             492..1229
                     /locus_tag="PKN52_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ99788.1"
                     /translation="MSDVPFSISARDLHKRYHLGDETITALAGVTLEVAAGEFVAVMG
                     PSGSGKTTLLHMLGLLDQPDSGVVEVAGRKTSGLSDDELTAVRRDRLGFIFQSFELIP
                     SLSARENILLPAEVAGRLAEGRAKLPGLTRLLGIEDRLDHRPRQLSGGQRQRVALARA
                     LINDPAVVLADEPTGNLDTKTGSEVLALLRRGVDASGWTVVMVTHDPSAAMVADRIVF
                     LRDGRVAAEVSAKGTEVRQAIDMFLAS"
     gene            1243..>1949
                     /locus_tag="PKN52_07290"
     CDS             1243..>1949
                     /locus_tag="PKN52_07290"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HNQ99789.1"
                     /translation="MFKALAVLVARYPRWVLGAWFAVSAVSLPFAARVGDVLSAQPEA
                     PSQGMAAAVQALLASEFQGGEEVTLVAVAHGVSVHAGSPEYAAALDDVRTRLSAIQGT
                     TAVRDYRAATGLDLLDEANDLSVLLIGLDASGLSEGKRVTQSVRAALDEVAELRFDLS
                     GGPATVVELEQVSERDARRAEVFGLPISLLILVVAFGAVVASLLPLLSAMTTIVTSLG
                     LLYFIGHQVEFAVFTET"
BASE COUNT          251 a          680 c          679 g          339 t
ORIGIN      
        1 agtgctgcgt cacgtgcggc ccgagcgcct cgcagatctc gctgtccttc tcgacgatga
       61 ggatttcgtt ccgccggtcg gccaggacga cactcagccg gcgctggttg ggcttgcccg
      121 ccacttccgt cttcgagacg aaggacttga ccttctccga ggccaggctg tgtttctggt
      181 agacttggtt ggcgtagtcc tgcagcgtca tcccggcagg ccgggcggtg aagtgcctga
      241 cggcgatgcg gaggtcggcg tcgtcgcact ggtagaggtc atcggcgtcg gcttcggcct
      301 cctgctagcc aacgtgatca cgaacggcgc cggcgccctc acgggtttcg tgctgcaacc
      361 ccgtctcccg tggtacttgg cgctactcgc cctgctcgcg tcgccggtcg tcgggctggt
      421 cgcgtcgctc ttccccgcca ggcgcgcggc gcgcatggcg ccaaccaagg ccttcgcctc
      481 ctggagtgag catgtctgac gttcccttct ccatctccgc ccgcgacctg cacaagcgct
      541 atcacctggg cgacgagacc atcacggctc tggccggcgt caccctcgag gtggcggcgg
      601 gtgagttcgt cgccgtgatg ggcccgagcg ggagcggcaa gaccacgctg ctgcacatgc
      661 tcgggctcct cgatcagccg gactccggcg tcgtcgaggt ggccgggcgg aagacgtcgg
      721 ggttgagcga cgacgagctg acggccgttc gccgcgaccg tcttgggttc atcttccagt
      781 cgttcgagct catcccgtcc ctgagcgcgc gggagaacat actcctcccg gccgaggtgg
      841 cggggcgctt ggccgaaggg cgggcgaagc tgccgggcct cacgcgcctg ctgggcatcg
      901 aggatcggct cgaccaccgc ccgcggcagc tatcgggcgg gcagcggcag cgggtcgcct
      961 tggcgcgcgc gctcatcaac gaccccgccg tggtgttggc cgacgagccc acgggcaacc
     1021 tggacacgaa gactggctcc gaggtcctcg cgctcctgcg gcgcggggtc gacgcgtcgg
     1081 gttggacggt ggtgatggtc acgcacgacc cgtcggccgc catggtggcc gaccgcatcg
     1141 tcttcctgcg agacggccgc gtggccgcgg aggtgagcgc caagggcacc gaggtccgtc
     1201 aggcgatcga catgttcttg gccagctgag cgcgttaccc tcatgttcaa agccctcgcc
     1261 gtcctcgtag cgcgataccc ccgctgggtg ctgggcgcct ggttcgcggt tagcgccgtc
     1321 tccctgccgt tcgcggcgag ggtcggggac gttctgagcg cgcaaccgga ggcgccgagc
     1381 cagggcatgg ctgccgcggt ccaagctctg ctcgccagcg agttccaggg tggcgaggag
     1441 gtcacgctcg tggcggtcgc ccacggggtc agcgtccacg ccggcagccc ggagtacgcg
     1501 gccgccctcg atgacgtcag gacccgcctc tcggccatcc agggcacgac agcggtgcgc
     1561 gactaccgcg ccgcgacggg cctcgacctg cttgacgagg ccaacgacct ctccgtgctc
     1621 ctcatagggc tcgacgcttc aggcctgagc gagggcaagc gcgtgacgca gagcgtgcgc
     1681 gcggccctcg acgaggtggc cgagttgcgc ttcgacctct cgggcgggcc ggcgaccgtg
     1741 gtggagctcg aacaggtgag cgagcgcgac gcccgccgcg ccgaggtctt cggcctcccg
     1801 atctccctgc tcatcctcgt cgtggcgttc ggcgctgtcg tggcgtccct gctgccgctc
     1861 ctgagcgcca tgaccaccat cgtcacgtcg ctcggccttc tgtacttcat cggccaccag
     1921 gttgagttcg ccgtgttcac cgagacgat
//
LOCUS       DAOQYE010000417         1948 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_68920_length_1948_cov_2.836239, whole genome shotgun sequence.
ACCESSION   DAOQYE010000417 DAOQYE010000000
VERSION     DAOQYE010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1948
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68920_length_1948_cov_2.836239"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(39..1403)
                     /locus_tag="PKN52_07295"
     CDS             complement(39..1403)
                     /locus_tag="PKN52_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNQ99790.1"
                     /translation="MDSDANVTRIAVIGAGPAGFYATEALLKGLGDAVSVDLIERLPT
                     PYGLVRFGVAPDHQKIKSVTRLYDRTLADPRVRYLGNVEFGKHLTIEDLRRHYHAVVV
                     TVGSPTDRRLAIPGEDLLGSMSATEFVAWYNSHPDFRDLEVPLAAKTAVIIGVGNVAI
                     DVTRVLAKTVAELGETDIADHALERLADSTVEDIVIVGRRGPAEAKFTTKELRELGEL
                     ANADIFVREEELEVGEASLAAIAGDVNAMKNLEVLRDFAKQTPEGKPRRVHIRFLLSP
                     LELRGNGHVSSVLFGKNRLEERQGGGLAAVATGETEEFHAELVFRSVGYQGTALSGIP
                     FDERAGVIPNEAGRVLTARGGAPVPGLYVAGWIKRGPSGVIGTNKACAMETVASLLAD
                     ELPVPPEPSVEALDALLADRGARPFTAADWAKLDAFETAAGTASGRPRVKLADVEAML
                     AAVG"
     gene            1519..>1948
                     /locus_tag="PKN52_07300"
     CDS             1519..>1948
                     /locus_tag="PKN52_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HNQ99791.1"
                     /translation="MEVTLPWPVRVQKYGGTSVGDVDRIGKVAARIERSVHASEKVVV
                     TVSAMGRTTDQLIGLAREVTARASRRELDVLLATGEQQSIALVAMALHERGIRARSFT
                     GAQAGFLTDSRHGSARILEVDPRPVLAALEDVDVVVVAGFQ"
BASE COUNT          273 a          680 c          691 g          304 t
ORIGIN      
        1 agccatgcgc cgcctcgtgc cgccggaagc cggtctcgtc agccgacggc cgccagcatc
       61 gcctcgacgt cggccagttt cacgcgcggt cgaccgctgg ccgtgccggc ggccgtctcg
      121 aaggcgtcca gtttcgccca gtcggccgcc gtgaagggtc gcgcgccgcg gtcggccaac
      181 agggcgtcga gcgcctcgac gctcggctcg ggcggcacgg gcagctcgtc ggccaacagg
      241 ctcgcgacgg tctccatggc gcacgccttg ttggtgccga tcacgccgct gggtccgcgc
      301 ttgatccagc cggcgacgta tagccccgga acgggcgctc cgccccgcgc cgtcagcacc
      361 cggccggcct cgttcgggat cacgccggcg cgctcgtcga aagggatgcc cgatagcgcg
      421 gtgccctggt agccgacgga gcggaagacg agctccgcgt ggaactcctc cgtctcgccc
      481 gtcgccacgg cggccagccc cccgccctgg cgctcctcga ggcggttctt gccgaacagg
      541 acgctgctca cgtgcccgtt gccgcgcagc tcgagcggcg agaggaggaa gcgtatgtgc
      601 acgcggcgtg gtttcccctc cggcgtctgt ttggcgaagt cgcgtagcac ctcgaggttc
      661 ttcatggcgt tgacgtcgcc cgcgatggcg gcgaggctgg cctcgcccac ctcgagctcc
      721 tcctcgcgca cgaagatgtc ggcgttggcc aactcgccca gctcgcgcag ctccttggtg
      781 gtgaacttcg cctcggcagg accgcggcga cccacgatca cgatgtcctc gacggtcgag
      841 tcggccaggc gctccagtgc gtggtcggcg atgtccgtct caccgagttc ggccaccgtc
      901 ttcgcgagca cgcgtgtgac gtcgatggcc acgttgccga cgccgatgat cacggccgtc
      961 ttggccgcga ggggcacctc gagatcccgg aagtcggggt gcgagttgta ccaggccacg
     1021 aactcggtgg ccgacatgct cccgaggagg tcctcgccgg gtatggcgag gcgcctgtcc
     1081 gtcggcgagc cgaccgtgac gacgaccgcg tggtagtggc gccgcaggtc ctcgatggtc
     1141 aggtgcttgc cgaactcgac gttgccgagg tacctgacgc gcggatcggc gagggtgcgg
     1201 tcgtagaggc gcgtgacgga cttgatcttc tggtgatcgg gggcgacgcc gaagcgcacg
     1261 aggccgtacg gcgtaggaag acgctcgatc aggtcgaccg acaccgcgtc gcccagcccc
     1321 ttgagtagcg cttccgttgc gtagaagccg gccgggccgg cgccgataac cgctatgcgg
     1381 gtaacgttgg cgtcgctgtc catgcctcga ttatggcagg cgccccaacc tggcaccggg
     1441 acgacgcggc aaggtaagct gcccggctat atggaagtcg ctccagcaca accaaccgag
     1501 gccttgcgcc gggacgccgt ggaggtgacc ctgccgtggc cggtcagggt gcagaagtac
     1561 ggcgggacga gcgtcggcga cgtggacagg atcggcaagg tggctgcccg catcgagagg
     1621 tcggtccatg cgagcgagaa ggtcgtcgtg accgtctcgg ccatggggag gaccaccgac
     1681 cagctcatcg gcctggcgcg cgaggtgacc gcccgcgcct ctcgccgtga gctcgacgtc
     1741 ctcctggcga cgggcgagca gcagtccatc gccctcgtgg ccatggccct gcacgagcgc
     1801 ggcatcaggg cgcgctcgtt cactggcgcg caagcgggct tcctgaccga ctcccgccac
     1861 ggctcggccc gcatcctgga agtcgacccg cgccccgtgc tcgccgccct ggaggacgtc
     1921 gacgtcgtgg tggtcgccgg tttccagg
//
LOCUS       DAOQYE010000418         1947 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_68971_length_1947_cov_2.807611, whole genome shotgun sequence.
ACCESSION   DAOQYE010000418 DAOQYE010000000
VERSION     DAOQYE010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1947
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68971_length_1947_cov_2.807611"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="PKN52_07305"
     CDS             <1..234
                     /locus_tag="PKN52_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99792.1"
                     /translation="RDTVVMNPGSVGMAVRLTAEGRGWYPAVAEYALIEVVGGQPSAH
                     LRKVRYDLDRLRDAVSRASMPRGDAYLARFRPG"
     gene            284..>1947
                     /locus_tag="PKN52_07310"
     CDS             284..>1947
                     /locus_tag="PKN52_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99793.1"
                     /translation="MASLRRNVTLALFLLVMPTLGFAQRPLAALLPQTTVFAVEMRQD
                     GFDPGVFQGLFADLDTSEAESAWEVLSDLFNGRGTDTGVAEPLLGLEGSFRDIFYDEC
                     PDLAAALFEGGADSWSAAVGVSVSRFAPEPELLLAVRGPTRSATARLLAGAVSCFDGR
                     RYGEEGRSAIYLVGDGGDSPALLAESGGVLLVATDPELLRGAIRRSNGAAEPSFLDSR
                     IAVATQGLGQMGWTATVNLAALADTLRLVRGAVPPEAAGLFERLVATLRIVGGFSWGL
                     TLDDAGLLVRTVTAWDEALAKSSGEGALLALLECADCGVGQPPLLPGATVAVEAGAFP
                     FQAFVAWIDSWLSDVAATGAFAEGEPTDVRTAVAEFLGVDLGEALLDWSDGSAYYLTL
                     GVLDTDLRNWVLGLPSVVVVPVTSEDAAWRGVRSWLSAAENLTSLVAEVAGEEEFAGA
                     LEFSQSVSVRESRHAGIDYLRVRSGPTLDVGLAVFGGNLVIGSPVSALLQTIDARDGA
                     PDKPASVTAGLLGLDRDAGRLVGYSVVDSRSFLQGLARIAELASGPA"
BASE COUNT          228 a          666 c          707 g          346 t
ORIGIN      
        1 cgcgacaccg tcgtcatgaa cccgggcagt gttggcatgg ccgtgaggct cacggccgag
       61 ggccggggct ggtacccggc cgtggcggag tacgcgctca tcgaggtcgt cggaggtcag
      121 ccgagcgcgc acctacgcaa ggtccgctac gacctcgacc gcctgcggga cgcggtgtcg
      181 cgagccagca tgccgcgcgg cgacgcctat ctcgcgaggt tccggcccgg ctagcttctc
      241 actcggcact aacttctcac tcggcgtgcg ctagcttgac tgcatggctt cgttacgaag
      301 gaacgtcacg ttggcgctct tcctcctggt catgccgacc ctcggcttcg ctcagcgacc
      361 cctcgccgca ctgctgcccc agacgacggt cttcgcggtc gagatgcggc aggacggctt
      421 cgaccccggc gtgttccaag gcctgttcgc cgatctcgac acgagcgagg ccgagtccgc
      481 gtgggaagtg ctgtccgacc tgttcaacgg acgtggcacg gatacgggcg tcgccgagcc
      541 cctgctcggc ttggagggct ctttccgcga catcttctac gacgagtgcc cggatctggc
      601 cgcggcgctg ttcgaaggcg gtgccgactc ctggtcggcc gcggtcggcg tgagcgtctc
      661 gcgcttcgcc ccggagcccg agttgctgct ggccgtgcgc ggccccacgc gcagcgcaac
      721 ggcacggctg ctggcaggcg ccgtgtcctg cttcgacggc cgtcgctacg gcgaggaggg
      781 tcgatcggcc atctacctcg tcggcgatgg gggagactcc ccggccttgc tggccgagtc
      841 cggaggagtg ctgctcgtcg ccaccgaccc cgagttgctg cgtggtgcca tccgccgttc
      901 caacggcgcc gccgaaccgt cgttcctcga ttcgcgcatc gctgtcgcca cccaggggct
      961 cgggcagatg ggttggacgg cgacggtgaa cctcgcggcg ctcgccgaca ccttgaggct
     1021 ggttcgtggc gccgtgccac cggaggccgc cggcctgttc gagcggctcg tggcgacctt
     1081 gcgcatcgtg gggggcttct cctggggtct gacgctcgac gacgccggct tgctggtgcg
     1141 caccgtcacc gcttgggacg aggcgctcgc caagtcgtcc ggagagggcg cgctcctggc
     1201 gctgctcgag tgcgccgact gcggcgtagg gcagccgccg ctcctgccgg gtgccaccgt
     1261 ggcggtcgag gccggcgcct tcccgttcca ggcgttcgtg gcctggatcg atagctggct
     1321 ctcggacgtg gccgccacgg gtgcgttcgc ggaaggggag ccgaccgacg tgcgcacggc
     1381 ggtggccgag ttcctgggag tggacctcgg cgaggcgctt ctcgactggt ccgacggctc
     1441 cgcctactac ctgaccctcg gggtcctcga caccgatcta cgcaactggg tccttggctt
     1501 gccgagcgtc gtggtcgtcc ccgtcacgtc ggaggacgcc gcctggcgtg gggtgcgttc
     1561 ttggttgtcg gcggccgaga acctcacctc gctcgtggcc gaggtcgcgg gcgaggaaga
     1621 gttcgccggg gcgctcgagt tctcgcagtc ggtgtccgtg cgcgagtcgc ggcatgccgg
     1681 gatcgactac ctgagggtga ggtccggtcc gaccctcgac gtggggctgg cggtgttcgg
     1741 cggcaacctg gtcataggga gcccggtctc cgccctgctg cagaccatcg atgcgcgcga
     1801 cggcgctccg gacaagccgg cgtccgtgac ggcggggctc ctcggtctgg accgcgacgc
     1861 aggtaggctg gttggttact ccgtcgtcga ctcgcgcagc ttcctgcagg gcttggcgcg
     1921 catcgccgag ttggcgtctg ggccggc
//
LOCUS       DAOQYE010000419         1941 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_69340_length_1941_cov_3.151113, whole genome shotgun sequence.
ACCESSION   DAOQYE010000419 DAOQYE010000000
VERSION     DAOQYE010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69340_length_1941_cov_3.151113"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="PKN52_07315"
     CDS             <1..248
                     /locus_tag="PKN52_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99794.1"
                     /translation="QDDSARAFDLFERLLGLPIPPELLAEGSLALIQAALSLGAAERA
                     RAAFEQAEGYLERCAPEARRDLGRVLEQLGHDLALLT"
     gene            complement(324..1118)
                     /locus_tag="PKN52_07320"
     CDS             complement(324..1118)
                     /locus_tag="PKN52_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99795.1"
                     /translation="MTGVYLRPLRDDLLPARHSAGARRRAGQLQAFLRPLHEVARVAL
                     RSEADVTLLVVDSRDWYKLARYPYGLPFARTAIGGAQANVIVAADHQPRLLQQFDGAM
                     LAAARAGVGAPAQLGEFIDLLVGHEWGHAVANLSGLRTRLKWLDELNATYLFVQALRA
                     TSQTDVLVRLAEWSRWQVAGTSTAAGDLAAFEYPRTRAGWDRLLWYQGVFAERAIELS
                     ARRGWDYLTELRSVLPPGDRGELARKLVELEPSFRPWFAVFGRKGK"
     gene            1299..1640
                     /locus_tag="PKN52_07325"
     CDS             1299..1640
                     /locus_tag="PKN52_07325"
                     /inference="COORDINATES: protein motif:HMM:NF018049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HNQ99796.1"
                     /translation="MSVWLWAGVAFLVGVGAGWGLSIYLRGRQPSEQRLRRLQAVLDR
                     FQGEVAQHFQESGQLITRLRADVESLYHHLEDGAARLTTEDAAQARLRQLEETSATQP
                     VPGPVERGTDH"
BASE COUNT          298 a          699 c          666 g          278 t
ORIGIN      
        1 cgcaggacga ctcggcacgc gcgttcgacc tgttcgagcg cctgctcggc ctcccgatac
       61 cgccggagct cctggcggaa ggctccctcg ccctgatcca ggcggcgctc agcctcgggg
      121 ccgccgaacg cgcgcgcgca gcgttcgagc aggcggaggg ctacctcgaa cgctgcgcgc
      181 ccgaagcgcg ccgcgacctt ggcagggtgc tcgaacagct cggtcacgac ctcgcgctcc
      241 tgacgtaggg tcgggtcggc gcatgagggc gagcctggag cgggcgggcc gccacgagcg
      301 cgccgacacc cgaacgcgtg ccctcacttg cccttccgac cgaacactgc gaaccatggt
      361 cggaagctgg gctcgagctc gacgagctta cgggccagtt cccccctgtc cccgggtggg
      421 aggacggacc gcagctccgt caggtagtcc cagccacgac gcgccgacaa ctcgatggcg
      481 cgctcggcga agaccccctg gtaccagagg agccggtccc agccggcccg cgtgcgcggg
      541 tactcgaacg cggcgaggtc gccggccgcg gtgctcgttc ccgccacctg ccacctcgac
      601 cactcggcca ggcgcaccag cacgtcggtc tgcgaggtgg cccgcagcgc ctgcacgaag
      661 aggtacgtcg cgttcaactc gtcaagccac ttgaggcgcg tgcgcaagcc gctcaggttg
      721 gcgacggcgt ggccccattc gtgcccgacc agcaggtcga tgaactcacc gagttgcgcc
      781 ggggcgccta cgcccgcgcg ggccgcggcg agcatggcgc cgtcgaactg ctgcagcagc
      841 cgcggctgat ggtcggcggc cactatcacg ttcgcctgag cgcctccgat ggccgtgcgg
      901 gcgaacggca ggccgtacgg gtaacgcgcc agcttgtacc agtcgcgcga gtcgacgacc
      961 aagagcgtca cgtcggcctc cgaacgtagc gcgacgcggg ccacctcgtg caacggccgc
     1021 aggaacgcct ggagctgccc cgcccgcctg cgcgcgcctg ccgagtggcg agccggcagg
     1081 aggtcgtcgc gcagtggcct caggtaaacg cctgtcaacg ccgttgccgg gttggcggag
     1141 gggctggccg cgggacctcc cgacgtgccg ccagccgccc cgcttggccg accatcgcgt
     1201 ttggctcgtc ctgggttcgc catgctggcc gcagtgtagg ggaaggggcc ctgaggaaga
     1261 cactgggacg atcgtgggtg cttgctatgc tcggcggcat gagcgtgtgg ttatgggccg
     1321 gagtggcctt cctggtcggt gtcggagccg gttgggggct ctcgatctac ctgcgcggcc
     1381 ggcagccgag cgagcaacgc ctccggcggc tgcaggccgt cctggaccgc ttccagggcg
     1441 aggtggcgca gcacttccag gagtcaggac agttgatcac gcggctgagg gccgacgtgg
     1501 agtccctcta ccaccacctc gaggacgggg ccgcgcggct gacgacggaa gacgccgcgc
     1561 aggcgcgctt gcggcagctc gaggagacct cggccacaca gccggtgccc ggccccgtcg
     1621 agcgcggcac cgaccactga ctcgacttcc aacccgacca ccgacccgac cgaatggtcc
     1681 gaccaactcg ctgagcaacg cccaccgtga ggctcattaa gccgctctgg cgcgaatttc
     1741 ctcatgtttc gaccccagtg gcgcgtgata gactccccgg cgacgtgaag cggctgttcc
     1801 taatcctcac cctgctgttg acttgcgtca ccgccgccgc tcaggagcgg cacaactacc
     1861 tcgcggtcaa cggcggcttg ctggatgagg ccggccccta ctacttcatc aaacaaggcg
     1921 acagctccaa cgccttcgcg a
//
LOCUS       DAOQYE010000420         1939 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_69468_length_1939_cov_3.228238, whole genome shotgun sequence.
ACCESSION   DAOQYE010000420 DAOQYE010000000
VERSION     DAOQYE010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1939
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69468_length_1939_cov_3.228238"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            247..585
                     /locus_tag="PKN52_07330"
     CDS             247..585
                     /locus_tag="PKN52_07330"
                     /inference="COORDINATES: protein motif:HMM:NF016147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HNQ99797.1"
                     /translation="MLRVSGSSRPNSVAGAIAALMRSEREVEVQAIGPQAVNQAVKAI
                     AISRSYLENDRLDLYAVPSFVRLELLGEERTAVRFLVTARQLPEREAPSGVDRTAPGG
                     GRQDETDEPD"
     gene            797..1840
                     /locus_tag="PKN52_07335"
     CDS             797..1840
                     /locus_tag="PKN52_07335"
                     /inference="COORDINATES: protein motif:HMM:NF013216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HNQ99798.1"
                     /translation="MARLRGGAFLAPTLHEVLSASPFDRADRALVTDLGYGAARRMLQ
                     LDAVLAPLLKRPDRLPAGVGDALRLGAFEILYRGTPPYAAVDAWVRIVKREAPGLAGL
                     VNAVLRRVRPLPDDAAPAVRASLPDWLFTSIAAAVEEHAQLACEAMLDPEPLWLTAFD
                     GAAARAALESDGASVVEHGLSPRGGPTSLRVMAPVSVDRLAAFTSGLVQPQNPASLRV
                     ALLLNPSPGSRVLDLASGRGVKTAVLAALGADVCAVELDPRRSADAERNLRRLGLAAR
                     HVGADLTGPLDLPPAPYVLLDAPCSGTGTPPVASVAWSAHMALRPLGSKMATRAAAGS
                     CVARMACDHCATR"
BASE COUNT          267 a          700 c          677 g          295 t
ORIGIN      
        1 ccgggaacct ctggtggacg tccgcccacg acgagtggcc cctcggtcct ctagtccagg
       61 acaccggcct gatggtctcc acgctgggcg aggatgaaga cggcgagatc tacgtcgccg
      121 actatgttgg agggcggctc tacaagttga tcgacgattg accaacgcag tgacgccggc
      181 acatgccaac cgcaccttgg aggcgcacaa tcaggtagtc ctgctggcgg gaggttcgtc
      241 atcgaaatcc tacgtgtctc cggctcgtct cgtccgaact ccgtggccgg cgccatcgcg
      301 gcgctgatgc ggagcgaacg cgaggtcgag gtccaggcca tcggccccca ggcagtcaac
      361 caggcagtga aggcgatcgc catctcccgc tcgtacctcg agaacgaccg gctcgacctg
      421 tacgccgtgc cgtcgttcgt gcgcctcgaa ctgctcggcg aggagcgcac ggccgtgcgt
      481 ttcctcgtta cggccaggca gttgccggaa cgggaggcgc cgagcggcgt ggaccggacc
      541 gcgccgggcg gcggacggca ggacgagacc gacgagccag attgacctca ccgtctgttg
      601 acgggcagcc tactgacgga accgactgcg ttgggagcgg tctcgaccac atagacggag
      661 cgcgtgtgac cgcgctgtaa cctggtctcg tgagcggtgc ggagtgagtg gcactaggcc
      721 cggcgcggcg aggccggctg acaggtcgcc cggcgcggct ccgggcgacg gaccgaggcg
      781 tacggcgctc gccgtcatgg cgcgcctgcg tggcggcgcc ttcctcgcgc cgacgctcca
      841 cgaggtactc agcgcttcac ccttcgaccg cgctgacagg gccctcgtga ccgacctcgg
      901 ttatggcgcc gcccggcgca tgttgcagct cgacgcggtg ctggcgccgc tgctcaagcg
      961 accggaccgg ctgcccgccg gggtgggcga cgccctgcgc cttggcgcct tcgagatcct
     1021 ctatcgcggc acgcccccct acgccgccgt cgacgcctgg gtgcgcatcg tcaagcgcga
     1081 ggctccaggg ttggccgggc tcgtgaacgc ggtactcagg cgggtccgcc cgctgcccga
     1141 tgacgccgcc cccgcggtgc gcgcctccct acccgattgg ctgttcacca gcatcgccgc
     1201 tgccgtcgag gagcacgccc agctggcctg cgaggccatg ctcgaccccg agccgctgtg
     1261 gctgacggcc ttcgatggcg cagccgcccg cgccgccctc gagtcggatg gcgccagcgt
     1321 cgtcgaacac ggcttgtcgc cccggggcgg ccccacctct ctacgggtca tggctcccgt
     1381 cagcgtcgac cgccttgccg cgttcacgag cggcctggtt cagccgcaga accccgcttc
     1441 cttgcgggtg gcgctgctgc tgaacccctc ccccggcagc cgcgtgcttg acctggccag
     1501 cggacgtggc gtgaagaccg cggtgctggc cgccttgggc gccgacgtgt gcgccgtaga
     1561 gctcgacccg cgccgttccg ccgacgccga gcgcaacctg cggcggctgg gactggcggc
     1621 caggcacgtc ggcgccgacc tcaccggccc gctcgacctt ccccccgccc cgtacgtgct
     1681 gcttgacgcc ccgtgcagcg gcacgggcac gccaccagtg gccagcgtgg cctggtcggc
     1741 gcacatggca ttgaggccgt tgggcagcaa gatggccacg cgcgcggccg cgggcagctg
     1801 cgtggcacgc atggcttgcg accattgcgc cacccgctga gcggtttgcg cccagctcag
     1861 gtgcacccag tcatgcgtgg cgggatcaaa agcgctgtag gcctgtgctt cgggggtgcg
     1921 ggcaacacgg taggcgagg
//
LOCUS       DAOQYE010000421         1935 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_69696_length_1935_cov_2.664894, whole genome shotgun sequence.
ACCESSION   DAOQYE010000421 DAOQYE010000000
VERSION     DAOQYE010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69696_length_1935_cov_2.664894"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..805)
                     /gene="aroC"
                     /locus_tag="PKN52_07340"
     CDS             complement(<1..805)
                     /gene="aroC"
                     /locus_tag="PKN52_07340"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HNQ99799.1"
                     /translation="MLRYLSAGETHGPALTVLLDGVPAGLPLVATDHIDPWLRRRQAG
                     YGRGQRMLIEQDHATFKGGVRAGRTTGGPLSIEILNRDWANWQDVMGAEPGNEPRKRA
                     LTQPRPGHADLAGGIKYHHKDLRDVLERASARETASRVAAGAVSLRLLEELGVDACAR
                     VMNLGGVECDGGMHWDRVADLDGSPLRTFDQSAEDRIIDLIDSAKENGDTLGGVVEAR
                     FRGVPIGLGSVMQWDRKLDGRLAQAVMSIPAVKGFEIGDGWRASGLPGSQ"
     gene            complement(840..>1935)
                     /locus_tag="PKN52_07345"
     CDS             complement(840..>1935)
                     /locus_tag="PKN52_07345"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNQ99800.1"
                     /translation="LADKLAGDQLPVRDGYHVYTVREPLGVTAHIVPWNGPLWEASRS
                     LAPALAAGNAAIIKPAQDALLGAIRLGEIAKAAGLPDGLVNVLPGPGSTVGEALLAHP
                     DVHGVTFTGSVAVGQLVMERAAKTMKRLVLELGGNAANIVFPDADLEAAVSGSIAAAF
                     SNAGQICVAGPRLLVHSDVLAPFTELFLARVARLSVGPGMEDHDITPVVSERHMRHVL
                     DMIEVGKAEGARLLAGGGRVTEGALANGYFVAPTVFDRVSSTMRIVREEVFGPVVTIQ
                     GFATEAEAVALANDTEYGLANGLWTNDLGRAHRMAARLQSGQVYVNEWFCGDVSCPAG
                     GYKLSGIGREEGQQAVDNYLQLKSVRMRVG"
BASE COUNT          254 a          687 c          704 g          290 t
ORIGIN      
        1 cctggctgcc gggcaggccg gaagcgcgcc agccgtcgcc gatctcgaag cccttgacgg
       61 cggggatgct catgacggcc tgtgcgagcc tgccgtccag cttgcggtcc cactgcatga
      121 cgctccccaa gccgatgggc acgccgcgga agcgcgcctc gacgacgccg cccagcgtgt
      181 cgccgttctc cttggccgag tctatgaggt cgatgatgcg gtcctcggcg ctctggtcga
      241 aggtccgcag cgggctcccg tcgaggtcgg ccacccggtc ccagtgcatg ccgccgtcgc
      301 actcgacgcc gccgaggttc atgacgcgcg cgcaggcgtc gacgccgagc tcctccagga
      361 gccgcagcga gacggcgccc gctgccacgc gcgaggccgt ctcgcgcgcc gaggcgcgct
      421 ccagcacgtc gcgcaggtcc ttgtggtggt acttgatgcc gccggcgagg tcggcgtggc
      481 ccggacgcgg ctgggtgagg gcgcgcttgc gcggctcgtt gcccggttcg gcgcccatga
      541 cgtcttgcca gttggcccag tcgcggttga ggatctcgat ggagaggggc ccgccggtcg
      601 tgcggccggc cctgacgccg cccttgaagg tggcgtggtc ctgctcgatg agcatgcgct
      661 gcccgcggcc gtagccggcc tggcggcggc gcagccaggg gtctatgtgg tcggtcgcca
      721 cgaggggcag cccggcaggc accccgtcca gtaggaccgt gagtgccggg ccgtgggtct
      781 cgcctgcgct taagtacctg agcatcggat tagggtagca cgcgcccaga accgaccgtt
      841 cagccgaccc gcatgcggac gctcttgagc tgcaggtagt tgtcgaccgc ctgctggccc
      901 tcctcgcgtc cgatgccgct gagcttgtag ccgcccgccg ggcacgagac gtcgccgcag
      961 aaccactcgt tgacgtagac ctggccggat tgcagccgcg ccgccatccg gtgggcccgc
     1021 ccgaggtcgt tcgtccagag cccgttggcc aggccgtact ccgtgtcgtt ggccaacgcg
     1081 accgcctcgg cttccgtcgc gaagccctgg atcgtgacca ccggaccgaa gacttcctcg
     1141 cggacgatgc gcatagtgct gctcacgcgg tcgaagacgg tcggcgccac gaagtagccg
     1201 ttcgccaagg cgccctcggt gacgcgcccg ccaccggcca gcagcctggc gccctcggcc
     1261 ttgcccacct cgatcatgtc gagcacgtgg cgcatgtggc gctcggagac caccggggtg
     1321 atgtcgtggt cttccatgcc cggcccgacg ctgagccgcg ccacgcgtgc gaggaacagc
     1381 tccgtgaagg gcgcgaggac gtcggagtga acgagcaggc gcggccccgc cacgcagatc
     1441 tggcccgcgt tggagaaggc cgcggcgatg ctgccgctga cggccgcctc gaggtcggcg
     1501 tcggggaaga cgatgttcgc ggcgttgccg ccgagctcga gcaccaagcg cttcatggtc
     1561 ttggccgcgc gctccatgac cagctgtccc acggccacgg agccggtgaa cgtgaccccg
     1621 tggacgtcgg ggtgggccag cagggcctcc ccgacggtgg agccggggcc gggcagtacg
     1681 ttcacgaggc cgtccggcaa gccggccgcc ttggcgatct cgccgagccg gatggcgccg
     1741 aggagggcgt cctgggcggg cttgatgatc gcggcgttgc cggccgccag cgccggcgcc
     1801 aggctgcgcg aggcctccca gagcggtccg ttccacggca cgatgtgagc ggtcacgccc
     1861 aggggctcac gcaccgtgta gacgtggtag ccgtccctca cgggcagctg gtcgccggcg
     1921 agcttgtccg ccagg
//
LOCUS       DAOQYE010000422         1929 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_70081_length_1929_cov_2.364995, whole genome shotgun sequence.
ACCESSION   DAOQYE010000422 DAOQYE010000000
VERSION     DAOQYE010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70081_length_1929_cov_2.364995"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(361..1431)
                     /locus_tag="PKN52_07350"
     CDS             complement(361..1431)
                     /locus_tag="PKN52_07350"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNQ99801.1"
                     /translation="MLHDLRRSRARRGSNALSEGRQAPSFVPPGYEVLNRLGSGQTAH
                     VYSARHAVFGDVALKLPRPELAHRPVLRRMFENEVAITTKLSSPQVVTAYEGFPTGPG
                     AFLSLEYCRGGTLDQLLLEKGLMPLDRCYRFILDVAAGLAHSHERQVLHRDVKPANVF
                     LTEQGTAKLGDFGTGMFMSDDSSDRVGTAFYMAPEVFEGNQAGVRSDIYSLGVLAYEV
                     IGGERPFTGNSYDALMVAHRTGVPKDLRALRHGLAPEVVRIVALAMMRDPAKRFGSAV
                     EFGQALAAATSTPMPRPDSASRSATPVAEKARVTGRGSRAVGRGSLEEESGGRHESGK
                     GAGERSEQPKRGFFGWFKRARD"
     gene            complement(1380..>1929)
                     /locus_tag="PKN52_07355"
     CDS             complement(1380..>1929)
                     /locus_tag="PKN52_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HNQ99802.1"
                     /translation="AILVTHAHGDHWGDTPELARRSGATVVGTAEVAAYAGKLGLRSH
                     AMNIGGSHAFPFGRVTLTPAWHSSSFPDGTYGGMPTGIVLELGGKRVYHAGDTALFSD
                     MRLIGREPIDLAFVPIGDNFTMGPQDAVEAVKLLNPRYVVPVHYGTFPLIAQDAAAFK
                     REVELSCSTTCVVLAPGEEVTL"
BASE COUNT          303 a          652 c          664 g          310 t
ORIGIN      
        1 ctccgtcgac gatgcgagcg cgcaggcctc ggatctccag ttgcttgtca ctcatacgaa
       61 tacctctcat ggaaggtccg gagctgttcg ttgccaaagt cctcgcgcga ccgccggccg
      121 ccggcaacaa cttggactcg ttccgattct aactcggatc ggttcttgcg gacgccctcg
      181 gaagacctag tgcgccgctt gcctttaagg ctaccaagcc ggcagacggt gcgccggtcg
      241 tcggtaaccg acggggcgcc tcggtcgcgg gcgcgctgtc caacgcgcca tcagtcttgg
      301 gcgcgcgcct tgtcttggga gcgccatcta tcttggacgc gccctcagcc gcacgcgcct
      361 tcagtcgcgc gcgcgcttga accagccgaa gaagcctcgt ttcggctgct cgctccgctc
      421 gccggcgccc ttgccgcttt cgtgccgccc accgctctcc tcctccagcg agccgcgacc
      481 gaccgcgcga ctgccgcgcc ccgtcacgcg cgccttctcc gccacgggcg tcgcggagcg
      541 ggacgccgaa tcgggtcggg gcatgggcgt cgaagtcgcg gccgcaagag cctggccgaa
      601 ctcgaccgcg ctcccgaacc gcttcgcggg gtcgcgcatc atggcgagcg ccacgatgcg
      661 gactacctcg ggagcaagcc cgtgccgcag ggcccggagg tccttcggca cgcccgtccg
      721 atgggcgacc atgagggcgt cgtacgagtt cccggtgaac ggacgctcac cgcctatgac
      781 ctcgtaggcg aggacgccga gggagtagat gtccgacctg accccggcct ggttgccctc
      841 gaacacctcc ggcgccatgt agaaagccgt gccgacccgg tcgctgctgt cgtcgctcat
      901 gaacatgccg gtgccgaagt cgccgagctt ggcggtgccc tgctcggtaa ggaagacgtt
      961 ggccggcttg acgtcgcggt gcaggacctg tcgctcgtgg ctgtgcgcca gccctgcggc
     1021 gacgtcgagg atgaagcggt agcaccggtc gaggggcatc aaccccttct cgaggagaag
     1081 ctggtcgagg gtaccgccgc ggcaatactc gagggagagg aacgcccccg gccccgtcgg
     1141 gaagccctcg tacgccgtga cgacctgcgg agaggagagc ttggttgtga tagcgacctc
     1201 gttctcgaac atacggcgca ggacgggtcg gtgggccagc tccggtcgcg gcagcttgag
     1261 agcgacgtcg ccgaacacgg cgtggcgagc cgagtagaca tgagccgtct gccccgaccc
     1321 gagtctgtta agtacctcgt aaccgggcgg gacgaaggac ggtgcctggc gaccctcgct
     1381 cagagcgtta cttcctcgcc gggcgcgaga acgacgcagg tcgtggagca cgagagctcg
     1441 acctcgcgct tgaacgcggc ggcatcctgg gcgatcagtg gaaaggtacc gtagtgcacg
     1501 ggaacgacgt accgagggtt gagcagcttt accgcctcga ccgcgtcctg cgggcccatg
     1561 gtgaagttgt cgccgatggg cacgaacgcc aggtcgatgg gttcccggcc gatgagccgc
     1621 atgtcggaga agagcgccgt atcgccggca tggtagacgc gcttgccgcc cagctcgagc
     1681 acgatcccgg tcggcatgcc gccataagtc ccatcgggga aggagctgga atgccaagcc
     1741 ggcgtgaggg tgacgcgccc gaacgggaac gcgtggctgc ccccgatgtt catggcgtgg
     1801 ctgcgcaggc ccagtttccc ggcgtaggcg gccacctcgg ccgtaccgac gacggtggcg
     1861 cccgagcgcc tcgcgagttc cggcgtgtcg ccccagtggt cgccgtgggc gtgtgtgacc
     1921 aggatcgcc
//
LOCUS       DAOQYE010000423         1927 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_70203_length_1927_cov_2.712607, whole genome shotgun sequence.
ACCESSION   DAOQYE010000423 DAOQYE010000000
VERSION     DAOQYE010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70203_length_1927_cov_2.712607"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1784
                     /locus_tag="PKN52_07360"
     CDS             <1..1784
                     /locus_tag="PKN52_07360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HNQ99803.1"
                     /translation="LAGILLAMFLAAIDQTIVSTALPKIVEDLGGLDRYAWVFTAYLM
                     VSTVLVPIYGKLADMFDRKKVELWAIGLFLTGSFLCGLAGEFGALPFLGDGMTQLIVF
                     RAFQGLGGAGLLGLAFIIIADLFPPAERGKYQGLVGSVFGIASVLGPLIGGFLTDHGG
                     GLIPGIAGWRWVFYVNLPFGILALWFVITRMPRLVPHGEKGKLDLLSVVLLFGGLGPL
                     VLGLQLDKAEYPWGSPTTLLLLGVGVVVLALFVARSLLAKSPILDMSLFRDDVFRTAN
                     IAVFFLGAAFLSMVAFLPLFMVNVLGVSATRAGLSLVPLSLGVTVGSTIAGQLVSRFG
                     HYRRILLIGGVVLLAGVYLLSSMGPDVSYWRVTLFMVIAGLGLGPTMPLYTLAVQNSV
                     DFRRIGQATSAVQFFRQIGGALGTALLGTVLTTGLVTAFATNMPAGAAPSSLGGPGGI
                     SAEGSTSGGPEAIAQGITEEFERTYDELAAAVRSGDDGQVSRVLAASALPDEAVAGVM
                     AGYQATKSNEAAKAAFVERLREEFTRQEQAVTTQVVRAVRVSFASAVTHVYFIVVFIV
                     FVGWVTTWFLPEKPLRKTHGAVAAVGE"
BASE COUNT          246 a          648 c          656 g          377 t
ORIGIN      
        1 tcctcgccgg catcctcctg gccatgttcc tcgcggccat cgaccagacg atcgtgtcga
       61 ccgcgctccc gaagatcgtg gaggacttgg gcgggctcga ccgctacgcg tgggtcttca
      121 ccgcgtacct catggtctcg acggtgctcg tgcccatcta cggcaagcta gctgacatgt
      181 tcgatcgcaa gaaggtcgag ctctgggcca tcggcctctt cctgacgggc tcgttcctgt
      241 gcggactggc gggcgagttc ggggcgctgc ccttcctcgg cgacggcatg acgcagctga
      301 tcgtcttccg ggccttccag ggcctcggcg gcgcgggcct gctcggcctc gcgttcatca
      361 tcatcgcgga cctgttcccg cccgccgagc gtggcaagta ccaggggctc gtcggcagcg
      421 tgttcgggat cgcgagcgtg ctcggaccgc tgatcggcgg cttcctcact gaccacggtg
      481 gcggcctcat cccggggatc gccggttggc gctgggtctt ctacgtgaac ctgccgttcg
      541 ggatcctagc tctctggttc gtcatcacgc gcatgccccg cctcgtcccg cacggggaga
      601 aggggaagct ggacctgctg tccgtcgtgc tcctcttcgg cggtctcggg ccgctcgtgc
      661 tcgggctcca gctcgacaag gcggagtacc cgtggggctc gccgaccacc ctcctcctgc
      721 tcggcgtagg cgtggtggtc ctcgcgctgt tcgtggctcg ctcgctcttg gcgaagagcc
      781 cgatcctcga catgagcctg ttccgtgacg acgtcttccg cacggccaac atcgccgtgt
      841 tcttcctggg cgcggccttc ctgagcatgg tcgcgttcct gccgctcttc atggtcaacg
      901 tactgggtgt ctcggcaacg cgggcgggcc tgagcctcgt gccgctctcg ctcggcgtga
      961 cggtcggttc gaccatcgca ggtcaactcg tctcgcgctt cgggcactat cggcgcatcc
     1021 tcctcatagg aggcgtcgtg ctgctggccg gcgtctacct gctctcgagc atggggccgg
     1081 acgtgtctta ctggcgggtg accctcttca tggtgatcgc gggcctcggc ctaggaccca
     1141 ccatgccgct gtacacgctg gccgtacaga actcggtcga cttcagacgg atcggccagg
     1201 ccaccagcgc cgttcagttc ttcaggcaga tcggcggcgc cctcggtact gcgctgctcg
     1261 gcaccgtcct gaccaccggc ctcgtgacgg ccttcgccac caacatgccc gccggcgccg
     1321 ctccgagtag cctcggcggg ccgggcggga tctcggccga gggatcgacg tcgggcggcc
     1381 cggaagccat agcgcagggc ataacggagg agttcgagcg gacctacgac gagcttgccg
     1441 cggccgtgag gtccggcgac gacggccagg tctcacgcgt tctcgctgct tcggccctgc
     1501 cggacgaagc ggtggctggg gtcatggccg gctaccaggc cactaagagc aacgaggccg
     1561 cgaaggccgc cttcgtcgaa cggttgcgcg aggagttcac gcgccaggag caggctgtca
     1621 ccacccaggt cgtgcgcgcg gtacgcgtgt cgttcgcatc ggcggtgacg catgtctact
     1681 tcatcgtcgt gttcatcgtc ttcgtcggct gggtcaccac ctggttcctg ccggagaagc
     1741 ccttgcggaa gacgcatggg gcggtggcgg cggtgggtga gtaaggtgct ccccctcgag
     1801 gccggctggc ctcgtgacgg tagtcgttac tgagccgaga tcccggttgg gtggatggta
     1861 ggggcggcca gagtggctag tcgaccgctc gcgggttacg cttgcctcgc gaccgaaggg
     1921 tggctag
//
LOCUS       DAOQYE010000424         1926 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_70260_length_1926_cov_2.672368, whole genome shotgun sequence.
ACCESSION   DAOQYE010000424 DAOQYE010000000
VERSION     DAOQYE010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1926
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70260_length_1926_cov_2.672368"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1820)
                     /locus_tag="PKN52_07365"
     CDS             complement(<1..1820)
                     /locus_tag="PKN52_07365"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HNQ99804.1"
                     /translation="MTVEVVAVEPLAPVPAGVEAVVPEPAANEPALGAPGAVRYEMVV
                     GLPGDAEVRNDSGAATVRVAPAPRLLLVSEDAAWARVLASAIEAQGVEVALTPPGALP
                     IDPAALAEYGAAALMNVPAAAITVDRQLALETAVFDLGLPLLLLGGESAFGPGGYYET
                     PLERLSPTSSLVPREAPELAIAFVLDRSNSMRQYAGDSVRLDIAKVATLKAHELLPEG
                     ARSTLIAFDSTARTLVPLGPAAEAEPFRAAVLELDPRGGTNLYPALVAAYEQLAGAGA
                     AAAHVIVMSDGLSQPGDFPGILGLLREANVTVSTIAIGPEADAEQLREVARLGGGTFH
                     ASADFSALPGIMAHEVLLQSGELTEERDVRPTWQGQRPPYLRAWPDEFPAVAGYVPTS
                     VKPEAKLHLSVTDDKGEAQPLLASWHYGAGEVVAFTAHAAGPWTREWLDEARYPVMWA
                     HLVRQLAKVPSGAATSVRLTRDGDLLEIATPAPDAVLTKPDGAVVPLRTRPAAAGYAA
                     EAFLDQTGVYLVASGGVTVGLEVPYLALLDSARADPDRLAAAALATGGGVVADLAEAA
                     APVGWYWPRSPAWPTAVSAALLVLLLDLTRRYAPDLLNWRV"
BASE COUNT          268 a          688 c          724 g          246 t
ORIGIN      
        1 acccgccagt tgagcaggtc gggcgcgtag cgccgggtga gatcgaggag gagaacgagc
       61 agggcggcgc tcacggcggt gggccatgcg ggcgagcgcg gccagtacca accgaccggc
      121 gcggcggcct ccgccaagtc ggccacgacg ccgcccccgg tggccagagc cgcggcggcg
      181 agcctgtcgg ggtccgcgcg cgcggagtcg agcagcgcca ggtacggtac ttccagcccg
      241 accgtcacac cgccggacgc cacgaggtag acgcccgtct ggtccaggaa cgcttcggcc
      301 gcgtagccgg ccgcggccgg tctggtccgt aggggcacga ccgcgccgtc tggcttggtc
      361 aggaccgcgt ccggagccgg ggtcgctatc tcgagcaggt ccccgtcgcg cgtcaggcgc
      421 acggaggtcg ccgcaccgct gggaaccttg gcgagctgcc tgacgaggtg cgcccacatg
      481 acggggtatc tcgcttcgtc cagccactcg cgggtccacg gtccagcggc gtgggccgtg
      541 aaggccacga cctcgcccgc gccgtagtgc cagctggcga gcaggggttg cgcctcgccc
      601 ttgtcatcgg tgacggagag gtggagcttg gcctccggtt tcacgctcgt cggaacgtac
      661 ccggccaccg cggggaactc gtcgggccag gcgcgcaagt agggcgggcg ctggccctgc
      721 caggtcggcc tgacgtcgcg ctcctcggtg agctcgcccg actgcagaag tacctcatgg
      781 gccatgatgc cgggcagcgc cgagaagtcc gcgctcgcgt ggaacgtgcc gccgccgagg
      841 cgggcgacct cgcggagctg ctccgcgtcc gcctcgggac cgatggcgat ggtggacacg
      901 gtcacgttcg cctcccgcag gagcccgagg atgccaggga agtcgcccgg ctgcgacagg
      961 ccgtcgctca tcacgatcac gtgcgccgcc gcggcgcccg cgccggccaa ctgctcgtag
     1021 gcagccacca gcgccgggta gaggttggtg ccgccccgcg ggtctagctc gagtaccgca
     1081 gcgcgaaacg gctccgcctc ggccgccggc ccaagcggca ccagggtgcg agcggtggag
     1141 tcgaaggcga tgagcgtgct cctggccccc tccgggagga gttcgtgggc cttaagggtg
     1201 gcgaccttcg cgatgtcgag ccgcacggag tcgcccgcgt actggcgcat gctgttggag
     1261 cgatcgagca cgaacgcgat cgccagttcg ggcgcttcgc ggggcactag gctcgacgtc
     1321 ggcgagagcc gctcgagggg cgtctcgtag tagccgcccg gtccgaacgc gctctcgccg
     1381 ccaagcagta ggaggggcag gccgaggtcg aacacggccg tctccagcgc gagctgccgg
     1441 tctacggtga tcgcggccgc tggcacgttc atgagcgccg cggcaccgta ctcggcgagg
     1501 gcggcggggt cgatcggcag cgcccccggc ggcgtgagcg cgacctcgac cccttgcgcc
     1561 tcgatggcgg acgcgaggac gcgcgcccag gcggcatcct cgctcacgag gagcagccgc
     1621 ggggcggggg ccaccctcac ggtcgccgcg cccgagtcgt tgcggacctc cgcatcgcca
     1681 ggcaacccga cgaccatctc gtaacggacg gcgccgggcg cgcccagcgc gggctcgtta
     1741 gcagccggct cggggaccac ggcctcgacc cccgcgggca cgggcgccag aggctcgacc
     1801 gcgacgacct cgacagtcac cttgcgcagt ggcccccggg cccgatcgac gacgagcgtg
     1861 agggcggctg gatgttctac tcgtcgggta ccaccggtcg gcccaagggg atcctcccgc
     1921 cgttgg
//
LOCUS       DAOQYE010000425         1926 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_70261_length_1926_cov_2.671833, whole genome shotgun sequence.
ACCESSION   DAOQYE010000425 DAOQYE010000000
VERSION     DAOQYE010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1926
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70261_length_1926_cov_2.671833"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1261
                     /locus_tag="PKN52_07370"
     CDS             <1..1261
                     /locus_tag="PKN52_07370"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNQ99805.1"
                     /translation="IRSGVKFHDGTPLTASDVAFSINRIKDPAVASPRADDFAVVTSI
                     DVVDDQTVVLHLSQPFSPLLAKLAATLNVVVSEATVAANGDLQSVVNGTGAFKMVEYL
                     PQTRMVFERNPDWWGTDADGNQLPYLDGMIAYFYPDPTARATAIQTGSVDWIEYVPAP
                     DLDVLNAARNVEVVGGLSANFRSLYLNVDVPPFDNALVRQALSYAIDEQAVVDLALFG
                     TGGVPATGTTIPASNFYGVQDSPYVGRDVEKARSLLAEAGYANGFAFDLYVTSTYDFL
                     RTPAEIIQANLADIGVTANIVAEDWSIYLPKVLEGDFTVTLLGESGQSDPDDFLYNVF
                     YTGNGGNLSHFSNAALDELLDRGRQVSDVNERRAVYQEAQHLIFELAPHVFLFHSAQY
                     EAIRPTVHGFEHYPNTSYISFSRTWLD"
     gene            complement(1438..1764)
                     /locus_tag="PKN52_07375"
     CDS             complement(1438..1764)
                     /locus_tag="PKN52_07375"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNQ99806.1"
                     /translation="MKKLDHTITGDGPTKQLLAFANGEVGSVVFGSARFEPGQRLPAH
                     GTGSHTGDEVSFIISGHLTGESGGVPFEIGAGEMSIIPAGEEHWSVAGPEPVTLVYVL
                     VERRGA"
BASE COUNT          322 a          701 c          596 g          307 t
ORIGIN      
        1 catccgctcc ggcgtcaagt tccatgacgg caccccgctg accgccagcg acgtggcgtt
       61 ctcgatcaac cgcatcaagg acccggccgt cgcctcgccg cgtgcggacg acttcgccgt
      121 cgtcacctcc atagacgtcg tagacgacca gaccgtcgtg ctgcacctca gccagccgtt
      181 cagcccgctg ctcgccaagc tcgcggccac gctcaacgtc gtcgtgtcgg aagcgacggt
      241 ggccgccaac ggcgacctcc agagcgtcgt gaacggcacc ggggcgttca agatggtcga
      301 gtacctgccg cagacccgca tggtgttcga gcgcaacccc gactggtggg gcaccgacgc
      361 cgacggcaac cagctcccct acctcgatgg gatgatcgcc tacttctacc cggatcccac
      421 cgcgcgcgcg acggccatcc agacgggtag cgtcgactgg atcgagtacg tgccggctcc
      481 cgacctcgac gtcctcaacg ccgcgaggaa cgtcgaggtc gtgggtgggc tgtccgccaa
      541 cttccgcagc ctctacctga acgtcgacgt gcctccgttc gacaacgccc tcgtgcgcca
      601 ggccctctcg tacgccatcg acgagcaggc cgtcgtcgac ctggccctct tcgggacggg
      661 cggcgttccg gctaccggca ccaccatccc ggccagcaac ttctacggcg tgcaggacag
      721 cccgtacgtg ggccgcgacg tcgagaaggc gcgctcgcta ctcgccgagg ccgggtacgc
      781 caacggcttc gcgttcgacc tctacgtcac gagcacgtac gacttcctcc gcacacccgc
      841 ggagatcatc caggcgaacc tggccgacat cggcgtgacc gccaacatcg tggccgagga
      901 ctggagcatc tacctaccca aggtgctgga gggcgacttc accgtcacgc tgctgggcga
      961 gtcgggccag agcgatccgg acgacttcct ctacaacgtc ttctacacgg gcaacggcgg
     1021 caacctgagt catttcagca acgccgctct cgacgagctc ctcgaccgcg gccgccaggt
     1081 ttccgacgtg aacgagcggc gcgccgtcta ccaggaagcg cagcacctca tcttcgagct
     1141 ggcgccgcac gtgttcctct tccatagcgc ccagtacgag gccatccgcc cgaccgtcca
     1201 tggtttcgag cactacccga acaccagcta catcagcttc agccgcacct ggctcgactg
     1261 agccttcgcc aacacgttct gaagagaggc cggacccgcg atgggtccgg cctctcggct
     1321 ggccggcggc gacgtgccga gtagctgcgg ctggtcaggc cgcgtacggc gttcgggctt
     1381 ggacggccag cctgggcgcg actgctcggg cgtgtgcggc caacccggtg caagcgctca
     1441 agcgccgcgg cgttccacca agacatatac gagggttact ggctccggtc cggctaccga
     1501 ccagtgctcc tcgccggcgg ggatgatgct catctctccc gcgccgatct cgaacggcac
     1561 gccgccgctc tcgccggtca gatggccgct gatgatgaac gagacctcgt cgcctgtgtg
     1621 agagcccgtg ccatgggccg gcagccgctg ccctggctcg aagcgggccg agccgaacac
     1681 cacgctcccc acctcgccgt tggcgaaggc gagtagctgt ttggtcgggc cgtcaccggt
     1741 gatggtgtgg tccagcttct tcaaggcgtt gccccttcct gcaaggtcgc gatcgaaacg
     1801 cggatagctg agcgcgcccc ttggcggggc gccagcaagg cacggagcgt ggcggaccgc
     1861 gccgtccagg catacgatgg ctcctagaga gcggctttgc cgcctacaga gtaaggagcc
     1921 ccacca
//
LOCUS       DAOQYE010000426         1922 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_70459_length_1922_cov_3.209427, whole genome shotgun sequence.
ACCESSION   DAOQYE010000426 DAOQYE010000000
VERSION     DAOQYE010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1922
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70459_length_1922_cov_3.209427"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="PKN52_07380"
     CDS             complement(<1..207)
                     /locus_tag="PKN52_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99807.1"
                     /translation="MSVGGASAERGKLFELAVARAATYLDRLKPGWREAPDPRELRAV
                     LELWYLRTRFAYRILLEDVVTALLA"
     gene            complement(204..1304)
                     /locus_tag="PKN52_07385"
     CDS             complement(204..1304)
                     /locus_tag="PKN52_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HNQ99808.1"
                     /translation="MEEPPLFGTVVVAGVGLIGGSIALGTRRRRLARRVIGFDRDPGV
                     LDAARGLGAIDEAQLAPGPWLREADLLIIAAPARAIVTLGEALLPHLADGAIVTDVGS
                     VKSPVVTALKRARFVGGHPMAGSERGGILNANAALIENAVWVLTPEPGATDPDALAAV
                     RGYVSALGARPIEVSPALHDRLVATVSHLPYLASVALTALVAEGEERSLKMLLAAGGF
                     RDLTRVASGDPLMSRDMVAGNRAAVRDALHAFRAQLDRLEGLLDDPEGLLTAGEAAKL
                     TRDGIPIVRRSLLPARHEAVLAVPDRPGELARITHALGEAGVNVKDIEVLGIRETGGA
                     IRLAFETSEELVRGSEALRAAGYDVHGRNGAA"
     gene            complement(1310..>1922)
                     /gene="aroF"
                     /locus_tag="PKN52_07390"
     CDS             complement(1310..>1922)
                     /gene="aroF"
                     /locus_tag="PKN52_07390"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:TIGR01361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HNQ99809.1"
                     /translation="SPYLFQGLGQEGLRILEDARAATGLPIVTEVTAPELVETVAESA
                     DMLQIGARNTQNFALLAEVGKAGKPVLFKRGMSTSISEFLQAAEYVLSQGNPNVVLCE
                     RGIRTFETSTRFTLDVSAVPVLKELSHLPVWVDPSHAAGRRGLVPSLALASAAAGADG
                     LIVEVHAKPEEAKSDAAQQLDDAEFGQLMTRLRGVVAALGKSL"
BASE COUNT          253 a          694 c          709 g          266 t
ORIGIN      
        1 ggcgagcagc gccgtcacga cgtcctcgag cagtatccgg taggcgaagc gcgtccgaag
       61 gtaccagagc tccaggaccg cgcgcagttc gcgggggtcc ggagcttccc gccagccggg
      121 cttgaggcgg tcgaggtagg tcgccgccct cgccaccgcc agctcgaaca gcttcccccg
      181 ctccgcggac gcgccgccca cgctcaagcc gcgccgttgc ggccgtgcac gtcgtagccg
      241 gcggcgcgca gcgcctcgct gccccgcacg agctcctcgc tggtctcgaa ggcgagccgg
      301 atggccccgc ccgtctcgcg gatgccaagg acctcgatgt ccttgacgtt cacgcccgcc
      361 tcgccgagcg cgtgcgtgat gcgcgccaac tcgcccggcc ggtcgggcac cgccaggacg
      421 gcctcgtgcc tggcgggtag gaggctgcgc cggacgatgg ggatgccgtc acgggtcagc
      481 ttggccgcct cgccggcggt cagcagcccc tccgggtcgt ccagcaaccc ctccaggcgg
      541 tcgagctggg ccctgaaggc gtgcagcgcg tcgcgaacgg ccgcgcggtt gccggccacc
      601 atgtcgcggc tcatcagcgg gtcgccgctt gccacgcgcg tcaggtcgcg gaagccgccg
      661 gccgccagga gcatcttgag tgagcgctcc tcgccctcgg cgacgagcgc agtcagcgcc
      721 acggacgcga ggtaggggag gtggcttacg gtcgcgacga ggcgatcgtg cagggctggg
      781 ctcacctcga tgggcctggc gccgagggcc gagacgtacc cccggacggc cgcgagagca
      841 tccgggtccg tggcgcctgg ttcgggcgtc aggacccaga cggcgttctc gatgagggcg
      901 gcgttggcgt tgaggatacc gccgcgctcg ctgcccgcca tggggtggcc gccgacgaag
      961 cgcgcccgct tcagggcggt gaccaccggg ctcttgacgc tgccgacgtc ggtgacgatg
     1021 gcgccgtcgg ccaggtgggg aagcagcgcc tcgcccaacg tgacgatggc gcgggccggc
     1081 gcggcgatta tcagtaggtc cgcctcgcgt agccacgggc cgggcgcgag ttgggcctcg
     1141 tcgatggcac caaggccgcg ggccgcgtcg agcacgccgg ggtcgcggtc gaagccgatc
     1201 acgcggcggg cgagccgccg ccgccgcgtg ccgagcgcga tggagccgcc gatgaggccg
     1261 accccggcga cgacgacggt cccgaagagc gggggctcct ccatcggcgt tacaggctct
     1321 tgccgagtgc tgcgaccacg ccgcgcaggc gggtcatgag ctgtccgaac tccgcgtcgt
     1381 cgagctgctg cgcggcgtcc gacttcgcct cttccggctt ggcgtgcact tcgacgatga
     1441 ggccgtccgc gccggccgcc gcgctggcga gggctaggga cggcacgagc ccgcgccggc
     1501 cagcggcgtg gctgggatcg acccagacgg gcaggtggga gagctccttc agcaccggca
     1561 cggcgctgac gtcgagggtg aagcgcgtgc tcgtctcgaa cgtccggatg ccccgctcgc
     1621 acagcaccac gttcgggttg ccctggctca acacgtactc ggccgcctgc aggaactcgc
     1681 tgatggacgt gctcatgccg cgcttgaaga gcacgggctt gccggccttg ccgacctcgg
     1741 cgaggagggc gaagttctgc gtgttgcgag cgccgatctg cagcatgtcc gcgctctcgg
     1801 ccaccgtctc gacgagctcc ggcgccgtga cctccgtgac gatcgggagc cccgtcgccg
     1861 ccctggcgtc ctccaggatc cgcaagccct cctggccgag cccctggaac aagtatgggc
     1921 tc
//
LOCUS       DAOQYE010000427         1921 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_70550_length_1921_cov_2.286174, whole genome shotgun sequence.
ACCESSION   DAOQYE010000427 DAOQYE010000000
VERSION     DAOQYE010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70550_length_1921_cov_2.286174"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1217)
                     /locus_tag="PKN52_07395"
     CDS             complement(<1..1217)
                     /locus_tag="PKN52_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99810.1"
                     /translation="MLLLLVYGCAAAQPLAADGGWARPTALPMASTRAPNAAPAAGPT
                     ERAVASVAVVALPDKVELVWADQEGVWVSGSPVAGPSEAHLTGQELLPGQMRMLASAE
                     GVRQVNAGAPAGELAVAWAQRDRLTGRYHHFLWWRGTVSPLLDDPLEVRFDFVSVAGV
                     PYAAAALRSAGAARLTLLPLAGGDRVVLHTTELSVRGVTYAQSDDGGLWLAWLEGKTE
                     RTELGLNAEWDAFALFVAPSGAASSVLKLGSADVSDERQRAAIGAAPGGAVVAWSDED
                     ANLRAALLAIDGDAAEGGATTVLATHASEAGRLPEPGRLLAVAWPYVYLVDGTTFKRL
                     HAPTLLEQPVGAVAVTNLLWSPVTVEGAEFSHAAADAPHAIAWFGREEGGAVRLFASD
                     DRAPMRVTWRDGLA"
     gene            complement(1288..>1921)
                     /locus_tag="PKN52_07400"
     CDS             complement(1288..>1921)
                     /locus_tag="PKN52_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HNQ99811.1"
                     /translation="DAVERQVIIRDLRLGHFDVLVGINLLREGLDLPEVSLVAILDAD
                     KTGFLRSERSLIQTIGRAARNVNGEVYLYADEVSDAMRLAMDETNRRRNKQLAYNEVN
                     GITPESVRKRVHDVIRGTEADDQEEAPAKLDPWERELVFDDLRQELATLETEMWEASE
                     ALDFERAAALRDRIREVEARLQGREVVITTIPGQAARNSASHRKSPKARA"
BASE COUNT          259 a          702 c          691 g          269 t
ORIGIN      
        1 gccaggccgt ctcgccacgt cacccgcatc ggcgcccggt catcgctcgc gaagaggcgc
       61 acggcgccgc cttcctcccg gccgaaccag gcgatggcgt ggggagcgtc cgcggcggcg
      121 tggctgaact ccgccccctc cacggtgacg ggcgaccaga gcaggttcgt gacggcgacc
      181 gcgccgaccg gctgctccag gagcgtgggc gcgtggagcc gcttgaacgt cgtgccgtcg
      241 accaggtaca cgtacggcca ggcgacggcg aggaggcgac cgggctcggg gagccgcccg
      301 gcctcggacg cgtgggtcgc caagacggtg gtggccccac cctctgcagc gtcaccgtcg
      361 atcgcgagca gcgcggcgcg caggttcgcg tcctcgtcgc tccaggccac gaccgcgccg
      421 cccggagccg cgccgatggc cgcccgctga cgctcgtcgc tgacgtcggc cgatcccaac
      481 ttcagcacgg acgaggcggc gccgctgggc gcgacgaaca gcgcgaacgc gtcccactcg
      541 gcgttcaggc ccaactcggt acgctccgtc ttgccctcga gccaggcgag ccagagcccg
      601 ccgtcgtcgg actgggcgta agtcacaccc ctcaccgata gctcggtggt gtgaagcacc
      661 acccggtcgc cgccggcgag cggcaggagg gtcaaccgcg ccgcgcctgc gctacgcagg
      721 gcggccgccg cgtacggcac gccggccacg gagacgaagt cgaagcgcac ctccagcggg
      781 tcatccaaga gcggtgacac ggtgccgcgc caccatagga agtggtgata gcgcccggtg
      841 aggcggtcgc gctgcgccca tgccacggcc aactcgccgg cgggtgcccc cgcgttgacc
      901 tggcgcacgc cctccgcgga cgcgagcatg cgcatctgac cgggcaggag ctcctggccg
      961 gtcagatgcg cctcggacgg ccccgcgacg gggctcccgg agacccatac gccttcctga
     1021 tcggcccaga cgagctcgac cttgtctggc agggccacta cggcgacgct cgcgaccgcg
     1081 cgttcggtcg gtccggccgc cggcgcggcg ttcggcgctc tggtcgacgc catcggcagc
     1141 gccgtcggtc tcgcccagcc gccgtcggcc gccagcggct gggcggccgc gcaaccgtat
     1201 acgaggagga gcagcaacgc gaggtagggc cggcgcgagt tccggtccct gtgccggcac
     1261 gcttctagga acgctcgccg gcgcacgtca ggcgcgagcc ttgggcgact tgcggtggct
     1321 ggcggagttc ctggccgcct gaccggggat ggtcgtgatc acgacctcgc gcccctgcaa
     1381 gcgcgcctcg acctcgcgga tgcggtcgcg cagcgcggcg gcgcgctcga agtcgagcgc
     1441 ctccgacgcc tcccacatct cggtctcgag ggtggccagt tcctgcctga ggtcgtcgaa
     1501 cacgagctcg cgctcccacg ggtcgagctt ggcgggcgcc tcctcctgat cgtccgcctc
     1561 ggtgccgcgg atgacgtcgt ggacgcgctt gcgcacggac tccggcgtga tgccgttgac
     1621 ctcgttgtag gcgagctgct tgttgcgccg cctgttcgtc tcgtccatcg cgagtcgcat
     1681 ggcgtccgag acctcgtcgg cgtacaggta gacctcgccg ttgacgttgc gggcggcgcg
     1741 accgatggtc tggatgaggg agcgctcgct ccgcaggaag cccgtcttgt cggcgtcgag
     1801 gatggccacg aggctgacct cgggcaggtc gagcccctcg cgcaacaggt ttatgcctac
     1861 caggacgtcg aagtggccga gccgcaggtc gcggatgatg acctggcgct ccacggcgtc
     1921 g
//
LOCUS       DAOQYE010000428         1919 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_70674_length_1919_cov_1.782725, whole genome shotgun sequence.
ACCESSION   DAOQYE010000428 DAOQYE010000000
VERSION     DAOQYE010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1919
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70674_length_1919_cov_1.782725"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..740
                     /locus_tag="PKN52_07405"
     CDS             <1..740
                     /locus_tag="PKN52_07405"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNQ99812.1"
                     /translation="RIEASASLRTFFVGMNTWRPPFDNVLVRQAMNYAIDPQLIVDTV
                     LSGQASVHPGVCGVGSFGYCPDCEPYAYDPIKAKELLTQAGYPNGFKVKFWSPRGKYI
                     KDLETSEAIVGQLEEVGIQVELYAPAWAEYWDNWLAGNMDIFYLSAGISFPDCDDLIG
                     YHLDSTRRGIYYNSPISDALIQREQQALDPEERAGIFAELSQFFVEEAPWVFLWDQNL
                     IYGVSSRILEFHALPVEEINYWELVVQ"
     gene            844..1761
                     /locus_tag="PKN52_07410"
     CDS             844..1761
                     /locus_tag="PKN52_07410"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ99813.1"
                     /translation="MAGYVLRRLLQFVLVLLVVTAHVFTLLHLSGNPASLLLPANATP
                     EQVREMSRSLGLEDPIHVQYFRYLANLFRGDLGTSYRMNLPVSRLIRIYLPHTRTLAG
                     LSILFATVISLVRGIAAALKRDTVIDLAATVGATVGQSMPTFWLAILLLLLFGVKLKW
                     LPVSGVGSWAHYVLPTVTMGWYSASFLTRTIRSNMLDVLRADYVQVARSKGLPERAVI
                     LRHAFRNVLLPVVTIWGLQLGTLLMGSVVTETVFAWPGVGRLSAESVLGRDYPVVLGL
                     IVVFGVMFGLLNVCIDISYAVLDPRVTYD"
BASE COUNT          308 a          633 c          599 g          379 t
ORIGIN      
        1 tgcgcatcga ggcctcggct agtctgcgca cgttctttgt cggcatgaac acctggcgtc
       61 cgccgttcga caacgtcctc gtgcgccagg cgatgaacta cgcgatcgat ccgcagctga
      121 tcgtcgacac ggtcctcagc gggcaagcct cggttcaccc tggagtctgc ggcgtgggca
      181 gcttcggcta ctgcccggac tgcgagccgt acgcgtacga tcccatcaaa gcgaaggagc
      241 ttctgaccca agccggctat cctaatgggt tcaaggtcaa gttctggtcg ccacgcggca
      301 agtacatcaa agacctcgaa acctcggagg cgatcgtcgg gcagctggag gaggtgggga
      361 tccaggtcga gctctacgcg ccggcgtggg ccgagtactg ggataactgg ctcgccggga
      421 acatggacat cttctacctc agcgccggca tctccttccc cgactgcgat gacctgatcg
      481 gctaccacct cgacagcacg cgccgcggga tctactacaa ctcaccgatc tcggacgctc
      541 tcatccagcg ggagcagcag gcgcttgatc cggaggaacg agccggcatc ttcgcggagc
      601 tgagccagtt cttcgtcgag gaggccccgt gggtcttcct gtgggaccag aacctgatct
      661 acggcgtaag ctcacgcatc ctggagttcc acgccctccc ggtcgaggag atcaactact
      721 gggagctggt cgttcagtaa cgcaccgacg cgcgagcgtc gtccctggag cgcgaccagc
      781 gcgctccggg ggcgacctcc gtgtcctgtt cggagaggat ctgcgccgcg cgacgggaca
      841 gtcatggcag ggtacgtact gaggcggctt ttgcagtttg tcctcgtgct cttggtggtc
      901 acggcccacg tgttcacgtt gctgcacctg agcgggaatc cggcctcgtt gcttcttccc
      961 gccaacgcca cgccggaaca ggtccgcgag atgagtcgat cgctcgggct ggaggatccg
     1021 atccacgtcc agtacttccg ctacctcgcg aacctattcc gcggcgacct cggaacgtcg
     1081 tatcggatga acctccccgt ctcaagactg atccgcatct acctcccaca cacccgcacg
     1141 ctcgccggcc tgtcgattct tttcgccacg gtgatctctc tcgtccgcgg gatcgcggcc
     1201 gccctgaagc gcgacacggt catcgatctc gcggccacgg tgggcgcgac ggtcggccag
     1261 tccatgccga ccttctggct ggccatcctg ctgctcttgc tcttcggtgt gaagctgaag
     1321 tggctgccgg tttccggggt tgggtcatgg gctcactacg ttctgccgac ggtcacgatg
     1381 gggtggtaca gcgcctcgtt cctcacgcga acgattcgct ccaacatgct cgacgtcctt
     1441 cgcgccgact acgtgcaggt ggctcgcagc aaggggctgc cggagcgcgc ggtgatcctg
     1501 cggcatgcct tccgcaacgt gctcttgccg gtggtcacga tctggggtct gcaactcgga
     1561 acgcttctga tgggatccgt cgtcacggaa acggtgttcg cctggcccgg ggtcggcagg
     1621 ctgagcgcgg agtccgtcct gggacgggac taccccgtgg ttctcggcct gatcgtcgtg
     1681 ttcggagtca tgttcggcct gctcaacgtg tgcatcgaca tcagttacgc cgtcctggac
     1741 ccgcgggtga cgtatgacta gcggagatgc gatggcgccg cggagaggac gagacagcac
     1801 cctgctccgg gtgcttcgca gacccaaagg agcgatcggt ctcctccttc tggtcggtct
     1861 gacgctgctc gcgatcctcg ccccctgcgt cgccacgcgc gatccgctcc agcagaacc
//
LOCUS       DAOQYE010000429         1915 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_70912_length_1915_cov_2.686022, whole genome shotgun sequence.
ACCESSION   DAOQYE010000429 DAOQYE010000000
VERSION     DAOQYE010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1915
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70912_length_1915_cov_2.686022"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            165..1004
                     /locus_tag="PKN52_07415"
     CDS             165..1004
                     /locus_tag="PKN52_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HNQ99814.1"
                     /translation="MKVAVVGAGTMGSGIAQTCALAGHEIVLVDTNPGAIDRAKETMR
                     GSLARMVKKETIEARDADDALGRVAFKHGITGLQDAGVVIEAVFESLPVKREVWTELA
                     SNTSEGALLATNTSSLSVTAIAAFSGRPERFCGMHFFNPVPMMALVEVVRGLRSSEET
                     VEQARAFTEGIGKTPIVCEDKPGFIVNRLLVPYINDAVHALSEGVANADDIDKAMRLG
                     ANMPIGPLALADLVGLDVCLAASESLLDEFGDPKFRVAPLLRQMVRAGKLGRKSGEGF
                     YGY"
     gene            1067..1897
                     /locus_tag="PKN52_07420"
     CDS             1067..1897
                     /locus_tag="PKN52_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNQ99815.1"
                     /translation="MTDGDEFEAGLEGDAPEDVSFEHLRYEVDGEVAIITVDRQEALN
                     ALNQDVLFELGLVFELAAADSAVRALVITGAGRAFVAGADIAALRDLGDAFAGRETSL
                     GGQEVTNTLASLPFPTVAAINGFALGGGLELALAADLRVAATGARLGLPEVGLGLIPG
                     FGGTQRLPRLIGVGRALDLILTGRHVQAEEALQLGLVNRVADDALAAAVALARQAARN
                     APIALGLAKEAVVRGVDVTLQQGLEIEADLFGMVATTADMREGTGAFLEKRAPEWTDS
                     "
BASE COUNT          286 a          634 c          715 g          280 t
ORIGIN      
        1 cgagcaaccg catcggcgac gccacgttcg tggagccggc ggtcctcgac gccgccatcc
       61 tcgacatgat cgaggcgact ctggaagcgt gcgtcggctt tcgggccggc gggtcggatg
      121 cttagcgaga tgggctgccg cgcgagcggc gggaggagtc aggcatgaag gtagccgtcg
      181 tcggcgcggg caccatgggc tccggcatag cgcagacctg cgcgttggcc ggtcacgaga
      241 tcgtgttggt cgatacgaac cctggcgcca tcgacagggc caaggagacg atgcgcggca
      301 gcctcgcgcg catggtcaag aaggagacca tcgaggcgcg cgacgccgac gacgcgctcg
      361 gccgggtggc cttcaagcac ggcatcacgg gtctgcagga cgccggagtc gttatcgagg
      421 ccgtgttcga gtcgctcccc gtcaagcgcg aggtctggac ggagcttgcg agcaacacgt
      481 cggagggcgc gctcctcgcc acgaacacct cgagcctgtc ggtcaccgcg atagcggcat
      541 tcagcggcag gccggagcgc ttctgcggca tgcacttctt caacccggtc cccatgatgg
      601 cgctggtgga ggtcgtacgc ggcctgcgct cgagcgagga gaccgtggag caggccaggg
      661 cgttcacgga ggggatcggc aagaccccca tagtctgcga ggacaagccg gggttcatcg
      721 tcaaccgcct cctcgtcccg tacatcaacg acgccgtgca tgccctgtcg gaaggggtgg
      781 caaacgcaga tgacatcgac aaggccatgc ggctcggcgc caacatgccc atcggcccgc
      841 tcgcgctggc cgacctcgtg gggcttgacg tctgcctggc cgcgagcgag tcgctgctcg
      901 acgagttcgg cgacccgaag ttccgcgtcg cgccgctcct gcgccagatg gtgcgggccg
      961 gcaagttggg ccgcaagagc ggcgaaggtt tctacggcta ctgatctcgt tgggcgccca
     1021 agccggcgcc tggccggggc cgcgcgccct ggcgggaggg agcgacatga cggacggtga
     1081 cgagttcgag gctggcctgg agggcgacgc cccggaggac gtgagcttcg agcacctgcg
     1141 ctacgaggtc gacggcgagg tggccatcat cacggtcgac cgccaggagg cgctgaacgc
     1201 cctcaaccag gacgtgctgt tcgagctcgg cctcgtcttc gagctggcgg cggccgattc
     1261 tgccgtgcgc gcgctggtga tcaccggcgc gggcagggcc ttcgtggcgg gggccgacat
     1321 cgcggccttg cgcgacctgg gcgacgcctt cgcgggtcgt gagacctcgc tcggcggtca
     1381 ggaggtcacc aacacgctcg cttccctgcc cttccccacc gtggcggcga tcaacggctt
     1441 cgcgctcggc ggcgggttgg agctggcctt ggcggccgac ctgcgcgtgg cggccactgg
     1501 cgcccgtctg gggctgccgg aggttgggct cgggctcatc ccgggcttcg gcggcacgca
     1561 gcggctaccg cgcctgatcg gcgtcgggcg cgcgctcgac ctcatcctca cgggtcgcca
     1621 cgtgcaggca gaggaggcgc tgcaactcgg tctcgtgaac cgggtagccg acgacgcgct
     1681 ggcggccgcc gtagcgctcg cgaggcaggc ggcgcggaac gcccccatcg cactcggact
     1741 ggccaaggag gccgtggtca ggggcgtcga cgtcacgttg cagcagggtc tggagatcga
     1801 ggccgacctg ttcggcatgg tcgccacgac ggccgacatg cgcgagggca cgggggcgtt
     1861 cctcgagaag cgcgcgcccg agtggacgga cagctgaacc cgggctgacc cgccg
//
LOCUS       DAOQYE010000430         1915 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_70920_length_1915_cov_2.447312, whole genome shotgun sequence.
ACCESSION   DAOQYE010000430 DAOQYE010000000
VERSION     DAOQYE010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1915
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70920_length_1915_cov_2.447312"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            203..670
                     /locus_tag="PKN52_07425"
     CDS             203..670
                     /locus_tag="PKN52_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99816.1"
                     /translation="MGGGTKFTLFLFTGYAVVSTLSRRRPAEDPTARRYLLAGVTCTA
                     MMLLLIAVTAFGLLIIEARTASPYQPPPTFADALLDASSAAAGGNLSSGILQTVTHRN
                     LSRGIRQHVDLYQYGMVWLMMAMLVGRVLPLVVLCRMATIKPHETPVALPPLV"
     gene            787..1449
                     /locus_tag="PKN52_07430"
     CDS             787..1449
                     /locus_tag="PKN52_07430"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HNQ99817.1"
                     /translation="MGIIPARAGSKRLPNKNLAPLDGRPLLSYTCRAALDSGVLSAVY
                     INTDSRELAAVAAEFGVACPVLRPAQLARDDTPMLDANRFLLQWLARRGETCDAVMIL
                     QPTSPLRTPEDIQQAWATYEANAPCAVVSVSPVRPASWLGRVGKDGRFEPLPGQDVIY
                     GLNGAIYIHDVEDYLADRRPTRTLVHPMPLARGVDIDTAEDLQYAEFLLRHAVPERCS
                     AL"
     gene            1502..>1915
                     /locus_tag="PKN52_07435"
     CDS             1502..>1915
                     /locus_tag="PKN52_07435"
                     /inference="COORDINATES: protein motif:HMM:NF015087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HNQ99818.1"
                     /translation="MRTYVVAEAGVNHDGALERALQLVDVAAAAGADAVKFQVFRAAE
                     LTTAAAATAEYQRQQTGAASQQQMLARLELSDADLARIAAHCRARNIEFLATPFGSAD
                     VDRLTALGVPALKIASTDLNNVPLLRRAAYTGLPLI"
BASE COUNT          314 a          698 c          590 g          313 t
ORIGIN      
        1 ccgcggaggt agcgaatcgc gtcgcccatc ggacaggctg agtacccagt cggcggggca
       61 gcggtttgcc cgcagcctag tgcaggtcgc gtgcgcaagt ggtgcgggaa taccgacaga
      121 agatctctcc gagaaagagg ttacagacgg cactaaggcg gccctagccg tagcgttggc
      181 tgtcggacca ctgggcgggt cggtgggcgg cggcaccaag tttaccctct tccttttcac
      241 cggctacgcc gtcgtctcca ccctttcacg ccggcgaccg gccgaagacc cgaccgcgcg
      301 acgctatctg ctggcgggcg taacctgtac tgccatgatg ctgcttctca tcgccgtaac
      361 cgctttcggc ctcctcatca tcgaggcgcg caccgcctcg ccataccaac caccacccac
      421 cttcgccgac gccctgctcg acgcctcctc cgctgcggcc ggcgggaacc tctcctccgg
      481 catccttcag accgtcactc accgaaacct ctccagagga atccgccagc acgtcgatct
      541 atatcagtac ggaatggtct ggctgatgat ggcgatgctg gtcgggcgcg tgctgccgct
      601 ggtcgtccta tgccgaatgg cgactatcaa gccgcacgag acgccggtgg ctctcccgcc
      661 gctggtctga ccggccaacg cagggccggc cactcacggc gtagcccggg actcgatcgt
      721 cgggcggggc acgccgccat cagcgggtcg actccgcgaa ccaacggtga gtgcaaaatg
      781 gatgtagtgg gaatcatacc ggcgcgagcc ggctcgaaac gcctcccgaa caagaacctc
      841 gccccgctcg acggccggcc gctgctgagc tacacctgcc gggccgccct tgatagcggc
      901 gtgctttccg ccgtatacat caacacggac agccgcgagc ttgccgccgt cgcggccgag
      961 ttcggtgtag cctgccccgt gctgcggccc gcacaactgg cccgcgacga cacgcccatg
     1021 ctcgacgcta accgcttctt gctacaatgg ttagcgcggc gcggcgagac ctgtgacgcc
     1081 gtcatgattc tccagccgac ctccccgctg cgcacacccg aagacattca gcaggcctgg
     1141 gccacctacg aggcgaacgc cccgtgcgcc gtcgtatccg tctcaccggt tcgcccggct
     1201 tcatggctgg gccgcgtcgg caaggacggt cgcttcgaac ctctgccggg acaggacgtt
     1261 atctacggcc tcaacggcgc catctacatc cacgacgttg aggactacct ggccgaccga
     1321 cgccccacgc gcaccctggt tcaccccatg ccactggcgc gcggcgtgga tatcgacacg
     1381 gcggaagacc tgcagtatgc cgagttcctg ctgcgacacg ccgtcccaga gcgctgctca
     1441 gccctctagt cccggtcatg gcgcgaacgt catgatcggc ccgcgggcag ttggtgccgg
     1501 ggtgcgcacg tacgtagtgg cagaagccgg cgtcaaccac gacggcgccc ttgaacgggc
     1561 gctccagctc gtggatgtcg ccgcggcggc cggcgcggac gccgtcaagt tccaagtctt
     1621 tcgggccgcc gagctgacca cggccgcggc ggcgaccgct gagtaccagc ggcaacagac
     1681 cggcgcggcg tcgcagcagc agatgctcgc gcggctcgaa ctgtcggacg ctgacctggc
     1741 tcgcatcgcc gcgcactgcc gcgcccgcaa catcgagttc ctggccaccc ccttcggttc
     1801 ggccgatgtg gatcggctga cggccctcgg agtgccggct ctcaagatcg cctcgacgga
     1861 cctgaacaac gtgccgttgc tacgccgcgc cgcctacacc ggtctgccgc tgatt
//
LOCUS       DAOQYE010000431         1915 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_70930_length_1915_cov_2.026344, whole genome shotgun sequence.
ACCESSION   DAOQYE010000431 DAOQYE010000000
VERSION     DAOQYE010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1915
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70930_length_1915_cov_2.026344"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(89..>1915)
                     /locus_tag="PKN52_07440"
     CDS             complement(89..>1915)
                     /locus_tag="PKN52_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99819.1"
                     /translation="PAELAARFGVDAALVSEAAQLTTERAATLEAAMAERTSAQVAIL
                     SAKLGALAPVGASAADSANQQALLDALADHWWLQYQDESGAWVDLDPSLPTSAPGSAL
                     TTAVETLDALGLTELPADLTHRVSLAVVLECVADGALSETTLVATEPLLPSRLLGQRV
                     SFTVLPGGLTEETAADPDALIEKLMSEQHWYPTINVADVTLTDLEFSPACETSPYSPS
                     SLGTAISDALFGAMDTLFGAGPAEDAVSALRLEFRVQRPGADDVVLARDLFDALGPAK
                     RAAGDLRLDEDDAARLAWRLKAMGETEALVTAGGVSRAFAADLAARALLAGESGLRAF
                     ADGAGASAPARPDAVAPSALLALVLERQKAQPTSLYQADANVFLRHTWLENGELGLAR
                     HQALDIVRADLASYSDAATARAERLAQGVADTNAEALIVRQLCEDDATAEYCLPYGNA
                     AERMAASPAAGPWLLLASPSEVDAALPTWSADARASLAADLRAGFAAVAPAAVEPADR
                     PATWWRVDPATGAVIGVSEDGRGDAAVEYGMLVNLVGMAFCTVGAVQAESVAAVTLCA
                     FGFATGTGALIGSAGPVTHLVGALMLASAVLYGLGGLGIPGR"
BASE COUNT          245 a          694 c          743 g          233 t
ORIGIN      
        1 ccgctaccgg gtggccgccc gcaccttcaa cacgcccgac agcacgacgg gccacatggg
       61 gttcagggta gccaggcccg cgccggcttc accgccccgg gatgcccaac ccgcctagcc
      121 cgtagagcac ggccgacgcg agcatcagcg cgcccacgag gtgggtcacg gggccggcgg
      181 agccgatcag cgccccggtg cccgtggcga agccgaacgc gcacaaggtc acggccgcca
      241 ccgactcggc ttggacggcg ccgaccgtgc agaacgccat gccgaccagg ttgacgagca
      301 tcccgtactc caccgcggcg tcgccgcgcc cgtcctcgct gacgccgatg accgcgccgg
      361 tggccgggtc gacgcgccac caggtggcgg gccggtcggc gggctcgacc gcggcgggcg
      421 ccacggcggc gaagcccgcg cgcaggtccg ctgccaggct cgcgcgggcg tcggccgacc
      481 aggtgggcag ggcggcgtcg acctcggagg gcgaggcgag cagcagccat gggccggcgg
      541 cgggcgaggc cgccatgcgc tccgccgcgt tgccgtaggg gaggcagtac tcggcggtag
      601 cgtcgtcctc gcagagctgc cgcacgatga gggcctcggc gttcgtgtcg gccacgccct
      661 gcgccaagcg ctcggcgcgc gcggtggccg cgtcggagta ggacgccagg tcggccctca
      721 cgatgtcgag ggcctggtgc cgcgccagcc cgagctcccc gttctctagc cacgtgtggc
      781 gcaggaagac gttcgcgtcg gcctggtaga gggaggtcgg ctgcgccttc tgccgctcca
      841 ggacgagcgc gagcagggcg ctcggtgcga cggcgtccgg tcgcgccggc gccgacgcgc
      901 ccgcgccgtc cgcgaacgcc ctcagcccgc tctcgcccgc cagcagcgcc cgcgccgcca
      961 ggtccgcggc gaacgcgcgg ctcacgccgc ccgcggtcac gagcgcctcc gtctcgccca
     1021 tggccttcag gcgccaggcg aggcgcgcgg cgtcgtcctc gtccagccgg aggtcgccgg
     1081 ccgcgcgctt ggcggggccg agggcgtcga agaggtcgcg cgcgagcacg acgtcgtcgg
     1141 cacccggccg ctgcacccgg aactcgagcc gcagcgcgct gacggcgtcc tcggcggggc
     1201 cggcgccgaa gagcgtgtcc atggcgccga agagggcgtc gctgatcgcc gtgccgagcg
     1261 acgaggggga gtacgggctc gtctcgcacg ccggtgagaa ctcgaggtcg gtgagggtca
     1321 cgtctgcgac gttgatggtc gggtaccagt gctgctcgct catgagcttc tcgatcaggg
     1381 cgtcggggtc ggcggccgtc tcctcggtca ggccgccagg gagcaccgtg aacgacacgc
     1441 gctggccgag cagtcgcgag gggaggagcg gctcggtggc cacgagcgtc gtctcgctca
     1501 gggcgccgtc ggccacgcat tcgagcacga cggcgaggct cacgcggtgg gtcaggtcgg
     1561 cgggtagttc cgtaaggccg agggcgtcga gcgtctccac ggcggtcgtg agcgcgctgc
     1621 caggcgcgct ggtcggcagg ctcgggtcga ggtcgaccca ggcgccggac tcgtcctggt
     1681 actgaagcca ccagtggtcg gcgagggcgt ccaggagggc ttgctggtta gccgagtccg
     1741 cggccgatgc accgactggc gcaagcgcgc cgagcttggc gctcaagatc gccacctggg
     1801 ccgacgtgcg ttcggccatg gccgcctcca gggtggcggc gcgttccgtc gtcagctgtg
     1861 cggcctccga gacgagagcc gcgtccacgc cgaagcgcgc cgcgagctcc gcggg
//
LOCUS       DAOQYE010000432         1911 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_71140_length_1911_cov_2.914871, whole genome shotgun sequence.
ACCESSION   DAOQYE010000432 DAOQYE010000000
VERSION     DAOQYE010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71140_length_1911_cov_2.914871"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1911)
                     /gene="pnp"
                     /locus_tag="PKN52_07445"
     CDS             complement(<1..>1911)
                     /gene="pnp"
                     /locus_tag="PKN52_07445"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HNQ99820.1"
                     /translation="QFGGRELVIETGKLAKQVSGSVTVRYGDTLVLVTAQMNDSASPV
                     PFLPLTVEYEERHYAIGKIPGSFMRREGRPGIQGTLNARIVDRQIRPLFPKGLRNEVQ
                     VIVTVLSADQVNDPAPLAAIGASAALAISDIPWNGPTACCTVGYADGEYLPNPSMRLL
                     HEGGSKLELTVAASKDAVLMVEAAANELSEDVMVGAIEFAQAQLAPVVALIERIRNEI
                     GQEKRGFAPAVELSQEALESVRRGSAGKLRDALLTEGKHERSEAVKALRDSIIAGLVP
                     DASTDGAAALVEEYKTAFDKVEKAELRRLILEENLRVDGRRSDQVRPIWIETGVLPMA
                     HGSAVFTRGETQVLGVATLGTGRDNRLVDDLTLDSSEEFMLHYNFPPYSTGEVKRLRG
                     TGRRELGHGNLARRALLDVMPSQEEFPYVIRVVGETLESNGSSSMATVCSGCLALMDA
                     GVPLRAPVAGIAMGLVMEGSSYRILTDILGSEDALGDMDFKVTGTAEGVTALQMDIKI
                     GGITAAVMREALAQARIGRLHILGEMAKTLAAPRTEISARAPRILQVKIPVDKIGTVI
                     GPGGKQIRELEAMGATVEVQEDGTVRIYSEDSTAARAVKAQIEALTASAEVGKEYDGV
                     VAKTTEFGAFIT"
BASE COUNT          262 a          637 c          702 g          310 t
ORIGIN      
        1 gcgtgatgaa ggcgccgaac tcggtcgtct tggcgaccac gccgtcgtac tccttgccga
       61 cctcggccga ggcggtgagc gcctcgatct gggccttgac ggccctcgcg gcggtcgagt
      121 cctcggagta gatgcgcacg gtgccgtcct cctgcacctc gacggtggcg cccatggcct
      181 cgagctcgcg gatctgcttg ccgccgggcc cgatgaccgt gccgatcttg tcgacgggga
      241 tcttgacctg caggatcctc ggcgcgcgcg cggagatctc ggtgcgcggc gccgcgagcg
      301 tcttggccat ctcgccgagg atgtgcagac ggccgatgcg cgcctgcgcg agcgcctcac
      361 gcatgacggc ggccgtgatg ccgccgatct tgatgtccat ctgcagcgcc gtgacgccct
      421 ccgccgtgcc cgtgaccttg aagtccatgt cgccgagcgc gtcctcggag ccgaggatgt
      481 cggtgaggat ccggtacgac gagccttcca tcacaagacc catcgcgatg ccggcgacgg
      541 gcgccctgag gggcacgccg gcgtccatga gggcgaggca gcccgagcag acggtggcca
      601 tggaggacga gccgttcgac tccagggtct cgccgaccac gcggatgacg taggggaact
      661 cctcctggct cggcatgacg tcgagcaggg cgcggcgcgc caggttgccg tggccgagct
      721 cgcgccggcc ggtccccctg aggcgcttga cctcgccggt ggagtacggc gggaagttgt
      781 agtgcagcat gaactcctca ctggagtcca gggtcaggtc gtcgacgaga cggttgtcgc
      841 ggcccgtgcc gagcgtggcc acgccaagga cctgcgtctc gccgcgcgtg aacacggccg
      901 agccgtgggc catcggcagc acgcccgtct cgatccagat cggacgcacc tgatccgagc
      961 gccgaccgtc gacccggagg ttctcctcga ggatgaggcg gcgcagctcg gccttctcga
     1021 ccttgtcgaa cgcggtcttg tactcctcga cgagcgcggc ggccccgtcg gtcgaagcgt
     1081 cagggacgag ccctgcgatg atggagtcgc gcagcgcctt cacggcctcc gagcgctcgt
     1141 gcttgccttc cgtgagcagg gcgtcgcgga gcttgccggc cgagccgcgc cggacgctct
     1201 cgagcgcttc ttgcgagagc tcgaccgccg gggcgaagcc gcgcttctcc tggccgatct
     1261 cgttccggat gcgctcgatg agggccacga cgggcgcgag ctgcgcctgc gcgaactcga
     1321 tggcgccgac catgacgtcc tcggagagct cgttggcggc ggcctcgacc atgagcaccg
     1381 cgtccttgct ggcggcgacg gtcagttcga gcttggagcc gccctcgtgg agcaggcgca
     1441 tgctcgggtt ggggaggtac tcgccgtccg cgtaaccgac cgtgcagcag gccgtcggcc
     1501 cgttccacgg gatgtcggag atggcgaggg ccgccgaggc gccgatggcg gcgagcgggg
     1561 cagggtcgtt gacctggtcg gccgagagca ccgtcacgat gacctggacc tcgttgcgta
     1621 ggcccttggg gaagaggggg cggatctggc ggtcgacgat gcgcgcgttc agggtcccct
     1681 ggatgccggg gcggccttcg cggcgcatga aggagccagg gatcttgcct atggcgtagt
     1741 ggcgctcctc gtactcgacc gtgaggggga ggaacggcac gggcgaggcg ctgtcgttca
     1801 tctgggccgt gaccagcacg agcgtgtcgc cgtagcgcac ggtcacgctg ccggatacct
     1861 gcttggcgag cttgcccgtc tcgatgacca gttcgcggcc gccgaactgg g
//
LOCUS       DAOQYE010000433         1910 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_71210_length_1910_cov_2.325606, whole genome shotgun sequence.
ACCESSION   DAOQYE010000433 DAOQYE010000000
VERSION     DAOQYE010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71210_length_1910_cov_2.325606"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="PKN52_07450"
     CDS             <1..201
                     /locus_tag="PKN52_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99821.1"
                     /translation="IEALTDMHRRKTGALIMAAVKTGALIAGADERKLSALTGYARAS
                     SKRLTAGRCSSTSSASCPSTHR"
     gene            156..836
                     /locus_tag="PKN52_07455"
     CDS             156..836
                     /locus_tag="PKN52_07455"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNQ99822.1"
                     /translation="MFLDELGELPLDAQVKLLRVLQESKVTRVGSTSDIDVDVRVVSA
                     TNRTVIEEVEAGRFREDLFYRLAVAVLKLPPLRERAGDLGLLIDELLKKENQDAEKAE
                     PGYVHKKLSTGARNLLLAHPWPGNVRELQLTLRRAAIWSDAVTISTEDIRESLLAVPH
                     AKRRDVLGRPLGDGLNLQELLKEVARHYLGRALDEAAGNKTRAAELVGAPSYQTLSNW
                     LKKYEVDA"
     gene            933..1280
                     /locus_tag="PKN52_07460"
     CDS             933..1280
                     /locus_tag="PKN52_07460"
                     /inference="COORDINATES: protein motif:HMM:NF025442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4258 domain-containing protein"
                     /protein_id="HNQ99823.1"
                     /translation="MPANDNRFETYRTEGRFTLTNHAKERMATRGLRADAIDAALAYG
                     RVAYVRGADIYAIGRKEIARYAEEGVDLSAYDGVQVVVTADGAILTVYRNRSFNGLRD
                     RRTYRRRPLALVA"
     gene            1362..>1910
                     /locus_tag="PKN52_07465"
     CDS             1362..>1910
                     /locus_tag="PKN52_07465"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNQ99824.1"
                     /translation="MILLADVSGSMAAEGKIEALNQSVREMLATFASTDDLRAEIHVA
                     IVTFGGEARLHTQLQPASAVKWSDMVADGGTPMGQAMTLAADLVDDKDKIPGRAYRPT
                     VVLISDGQPTDSWQTGLDRMTKQGRAQKADRMALAIGGDADTDMLRRFLNDEKKQVFV
                     AADARRIKDFFQFVTMSVTARSR"
BASE COUNT          361 a          677 c          584 g          288 t
ORIGIN      
        1 atcgaggccc tgacggacat gcacaggcgc aaaaccggcg cgctgatcat ggcggcggtc
       61 aaaaccggcg cgctcatcgc aggcgcagac gaacgtaagc tttcggcgct gaccggctac
      121 gcaagggcgt cttcgaagag gctcacggcg ggacgctgtt cctcgacgag ctcggcgagc
      181 tgcccctcga cgcacaggtg aagttgctcc gcgtcctcca ggagtcgaag gtcacgaggg
      241 tcgggtccac gtcggacatc gacgtggacg ttcgcgtggt ctcggcgacc aaccgcaccg
      301 tcatcgagga agtggaggcc gggcgctttc gcgaggacct cttctacagg ctcgccgtcg
      361 cggtcttgaa gctgccacca ctccgcgagc gagccggcga cttgggcctc ctgatcgacg
      421 agctgctgaa gaaggagaac caggacgccg agaaagccga gcctggatac gttcacaaga
      481 aactttctac tggcgcaaga aaccttcttc tcgctcaccc atggccgggc aacgtccgcg
      541 agcttcaact cacgcttcgg cgcgcggcca tctggagcga cgcagtcacc atctcgaccg
      601 aggacatccg cgagtccctg ctcgccgtgc cccacgcgaa gcgccgcgac gtcctcggtc
      661 gtcccctcgg cgacggcctc aacctacaag aattgttgaa ggaagtggcg cgacactact
      721 tgggccgcgc gctcgatgaa gccgccggaa acaagacgcg ggcagcggag ttggtcggcg
      781 ctcctagcta ccagacgctg tcgaactggc tgaagaagta cgaggtcgac gcgtgacctt
      841 ctcctcgccc tcgagtcctg gcacgccggc tgctcatgcg gtcttcgcca acggcaacca
      901 cgggaggttg tcgttgaacc cggagactca gcatgcccgc caacgacaac cgcttcgaga
      961 cctaccgcac cgaaggccgc ttcactctca ccaaccacgc caaggagcgc atggcgacgc
     1021 gcgggctccg cgccgacgcc atcgacgccg cgctggccta cgggcgcgtc gcctacgtcc
     1081 gtggcgccga catctacgcg atcggccgca aggagatcgc tcgctacgcg gaggagggcg
     1141 tcgacctctc cgcatacgac ggcgttcagg tcgtcgtcac cgccgacggc gccattctca
     1201 ccgtgtaccg caaccgctcc ttcaacgggc ttcgcgaccg ccgcacatac cgccgccgcc
     1261 ctctcgccct cgtggcgtag gccgcaccgc gagaccaacc ggagaagacc atgacctcac
     1321 agctcaaaga gttcacctcg tcggccgccc gaccgctccc cgtgatcctc ctcgccgacg
     1381 tcagcggaag catggccgcc gagggaaaga tcgaggctct caaccagtcc gtccgcgaga
     1441 tgctcgcaac gttcgcatcg accgacgatc ttcgcgccga gatccacgtc gccatcgtta
     1501 ccttcggcgg cgaggcgcga cttcacaccc agcttcaacc cgcgagcgcg gtgaagtgga
     1561 gcgacatggt ggccgatggc ggcacgccga tgggccaggc gatgacgctc gcagccgacc
     1621 tcgtcgacga caaggacaag atcccggggc gcgcctaccg ccccaccgtc gttctcatct
     1681 ccgatggtca gcccaccgac tcttggcaga cgggcctcga ccgcatgacc aagcaaggtc
     1741 gcgcgcagaa ggcggaccgc atggcactgg cgatcggcgg cgacgccgac accgacatgc
     1801 tccgacgctt cctgaacgac gagaagaagc aggtcttcgt cgctgcggat gcgcgccgca
     1861 tcaaggactt cttccagttc gtcacgatgt ccgtcacggc gcgttcgcgg
//
LOCUS       DAOQYE010000434         1909 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_71273_length_1909_cov_2.531284, whole genome shotgun sequence.
ACCESSION   DAOQYE010000434 DAOQYE010000000
VERSION     DAOQYE010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71273_length_1909_cov_2.531284"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="PKN52_07470"
     CDS             <1..190
                     /locus_tag="PKN52_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99825.1"
                     /translation="PDGAVQSVTGPARDFCLLVTHRRHRDDLDLVATGADADRWLDIA
                     QAFAGPPGSGRTPTRGAT"
     gene            190..408
                     /locus_tag="PKN52_07475"
     CDS             190..408
                     /locus_tag="PKN52_07475"
                     /inference="COORDINATES: protein motif:HMM:NF018938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HNQ99826.1"
                     /translation="MSDTARGAAGSATEPLIVANCSGFYGDRLSAMREMLDGGPVDVI
                     TGDYLYVYSASNRGGAAATSQYTLLGGL"
     gene            494..1423
                     /locus_tag="PKN52_07480"
     CDS             494..1423
                     /locus_tag="PKN52_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99827.1"
                     /translation="MRVRFRWAFMALAALASPLTTAALAQVCQAELSADFDQASLGRA
                     PTGLDAAAALRRAVELVEPALPPLQYDKPVPVDTSAAGYANVRYLVERKLLPKSWTEG
                     ELTGETWAAMLDAFLAWYQLSPGRFDAPADVAELLADMSEVLGRVSRAIRPAGLLATD
                     PSDGRRTSFWAIIWNWTVYPRLLVVRPDPDVGTRPADALAALSNCAVHVTAYISAPEE
                     TAKSLFLTHNSSRMYVVASQPGKNGFWPYEVAPGDELAAFAFELPDLSGVHVYAAVFD
                     GPEVGFGTLLGLMWRVRTNVAPTALLGFLSTPQ"
     gene            complement(1532..>1909)
                     /locus_tag="PKN52_07485"
     CDS             complement(1532..>1909)
                     /locus_tag="PKN52_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99828.1"
                     /translation="YLRLADALNERAAALPRDAQGYTLIGAVAGATQPNAIRELRATL
                     PSSLLLVPGYGAQGGSAADAALAFDTNGWGAVVNASRSITYPAAAVTAMSLKEVGAAA
                     RAAAAGARADLREALAARSARLE"
BASE COUNT          250 a          700 c          666 g          293 t
ORIGIN      
        1 acccgacggc gccgtgcagt cggtgaccgg accggcgcgc gacttctgcc tgctggtcac
       61 ccaccgccgc caccgcgacg acctggacct ggtcgccacc ggcgcggacg cggaccggtg
      121 gctcgacatc gcccaggcgt tcgccgggcc gcccggctcg ggacgcacac cgacaagagg
      181 ggcgacgtga tgagcgacac cgcgcgcggc gcggccggct cggcgaccga gccgctgatc
      241 gtcgccaact gctccgggtt ctacggcgac cgcctgtcgg ccatgcgcga gatgctcgac
      301 ggcggaccgg tcgacgtgat caccggcgac tacctgtacg tctactcggc gagcaaccga
      361 gggggagcgg cggcgaccag ccagtacacg ctactgggcg gcctgtaggc gacgggcgcg
      421 ccggtcccgc ggctggcgac cggcgacccg gctcctctca ccacccccac gcatgggccg
      481 gtacactcgg cccatgcgtg ttcgtttccg gtgggcgttc atggccctcg cagccctcgc
      541 ctcaccgttg acgaccgcgg cgcttgctca ggtctgccag gccgagctga gcgccgactt
      601 cgaccaggct tcgctcggta gggcgccgac cggcctggac gccgctgcgg cgctgaggcg
      661 cgccgtcgag ctcgtcgaac ccgccttgcc gcccctgcag tacgacaagc ccgttccggt
      721 ggacacctcg gccgccggct acgcgaacgt caggtacctg gtcgagcgca agctgctccc
      781 gaagagttgg acggaagggg agttgacggg cgagacctgg gcggccatgc tcgacgcgtt
      841 cctcgcctgg taccaactgt ctcccggccg cttcgatgcc ccggccgacg tggccgagtt
      901 gttggccgac atgagcgagg tgctcggccg ggtgtcgcgc gcgatccggc cggccggcct
      961 gctcgccacg gatccgtccg acggtcgccg cacgtcgttc tgggccatca tctggaactg
     1021 gaccgtctac ccgcgcctgc tcgtcgtgcg cccggatccc gatgtcggca ctcggccggc
     1081 cgacgccctg gctgcgctct ccaactgcgc cgtgcacgtt accgcctaca tctccgctcc
     1141 ggaggagacg gccaagagcc tgttcctcac gcacaacagc tcgcgcatgt acgtcgtcgc
     1201 cagccaaccg ggcaagaacg gcttctggcc gtacgaggtc gcgcccggcg acgagttggc
     1261 cgcgttcgcg ttcgagctcc ccgacctgag tggcgtgcac gtttacgcgg ccgtcttcga
     1321 cggccctgag gtcggcttcg gaaccctcct cgggctcatg tggcgcgtgc ggaccaacgt
     1381 ggcgcccacg gccttgcttg ggttcctcag caccccgcag tgaagtcagg accacttggc
     1441 cggccgcgag ggcttccttc ggaaagcgcc tcttgcagag gtctcctggt ggaggtctcc
     1501 tggcggaggt ctcttgaagg agtgggctgg cctactctaa gcgcgcgctc ctggccgcca
     1561 acgcctcgcg caggtccgcg cgcgcgccgg cggcggcggc ccgcgccgcc gctccgactt
     1621 ccttcaagct catcgccgtc acggcggcgg ccgggtacgt gatgctccgc gacgcgttga
     1681 cgacggcgcc ccagccgttc gtgtcgaagg cgagggccgc gtcggccgcg ctcccgccct
     1741 gggcgccgta gcccggcacc agtaggaggc tggaaggtag cgtggcgcgc agctcgcgga
     1801 tggcgttcgg ttgagtagct ccagcgacgg ctccgatcag cgtgtagccc tgggcgtcgc
     1861 gcggcagagc ggcggcgcgc tcgttcaaag cgtccgcgag gcggaggta
//
LOCUS       DAOQYE010000435         1902 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_71705_length_1902_cov_2.708717, whole genome shotgun sequence.
ACCESSION   DAOQYE010000435 DAOQYE010000000
VERSION     DAOQYE010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71705_length_1902_cov_2.708717"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="PKN52_07490"
     CDS             <1..271
                     /locus_tag="PKN52_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99829.1"
                     /translation="IAAWNLLYAIAPVHDRSLYIGLSNTVLALPSLAPVAAGILIPFL
                     GTPALLGLAALMAAITVSFSFRFGALRAADARALSAATSGAEAEA"
     gene            complement(282..611)
                     /locus_tag="PKN52_07495"
     CDS             complement(282..611)
                     /locus_tag="PKN52_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HNQ99830.1"
                     /translation="MKLILAIVQDADSSRLQEALGENGFQSTKLASTGGFLREGNTTV
                     LIGVQDHKVPEVKEIIHATCWERTKVVTAGSPIHALEGVFASQPMEVPVGGAIVFVLS
                     TDEFVRL"
     gene            723..>1902
                     /locus_tag="PKN52_07500"
     CDS             723..>1902
                     /locus_tag="PKN52_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99831.1"
                     /translation="MPAADVGGISKRIAERLEMDPQRIHTLLNGRKGPVTKPVLPDKA
                     AAIAEVFAEAGVAVEVVPASDAVVSFVSGGEQGAGAQVEKQFTHDSETRFDADDEPDF
                     GPEPELELEPEFEPEEYSEEYPEAPYPETPRPAWDTSSAWAVEDTGSWSALRPVATER
                     SARDPDPDGSEDRPGGRNDAWSAEPEYTRRTTPGRPGAGWPLPEGSAPEAADDGAGAA
                     DPEEEADRLGPRSPASDISVFTTSTRWVPSPHDGYVSDDVSDDAPGDEGYPAAADPDR
                     GWSGGGPPSAGRGTGVARTSSNAYNGAQWIQPPARAEAPRLRPYLVWSLIASVAVFLL
                     LQYVMAGRMEGAPALGTFELGMNAYRSGDFGAARRSLEPEAEAGNHLANDANENGVTD
                     S"
BASE COUNT          294 a          640 c          679 g          289 t
ORIGIN      
        1 gatcgccgcg tggaacctcc tgtacgccat cgcgcccgtg cacgaccgct cgctgtacat
       61 cggcctctcc aacacggtgc tcgcgctgcc gagccttgcc ccggtcgcgg ccggcatcct
      121 cataccgttc ctcggcacgc cggcgctctt ggggctggca gcgctcatgg ccgccatcac
      181 ggtctcgttc tccttcaggt tcggggcgct acgggcggcc gacgcgcggg cgctctcggc
      241 ggcaacgtct ggcgcggaag ccgaggctta ggacaccgcg cttacagcct gacgaactcg
      301 tcggtggaga gcacgaacac gatggcgccg ccgacgggca cctccattgg ctggctcgcg
      361 aacacgccct cgagcgcgtg gatgggagag ccggccgtga cgaccttggt ccgctcccag
      421 caggtggcgt ggatgatctc cttcacctcg ggaaccttgt ggtcctggac gccgatgagg
      481 acggtcgtgt tgccctctcg taggaagccc ccggtcgaag ccagcttggt ggactggaag
      541 ccgttctcac ccaaggcttc ctggagacgc gaggagtctg cgtcctgcac gatggcaagg
      601 atcagtttca tggtgtgaac atgctagcag acaggggctt aggttggtga gagtacaatg
      661 acgccaggac gggaaggtgc ggtttgagcg aacggcgctt cgttgttgag gtcactcgcc
      721 cggtgcccgc cgccgacgtc ggggggatct ccaagcgtat cgctgagcgc ttggagatgg
      781 atccgcaacg catccatacc ttgctcaacg gccgcaaggg cccggtcacc aagccggtgc
      841 tacccgacaa ggccgccgcc atcgccgagg tgttcgcaga ggccggcgtc gccgtggagg
      901 tggtgcctgc gtcggacgcc gtcgtctcct tcgtgtcggg gggagagcag ggcgccggcg
      961 ctcaggtgga gaagcagttc acccacgata gcgagacgcg cttcgatgcg gatgacgagc
     1021 cggacttcgg gccggagccg gagttggagc tcgagccaga gttcgagccg gaggagtact
     1081 cggaggagta cccggaggcc ccctacccgg agacaccccg cccggcctgg gacacctcgt
     1141 cggcttgggc cgtcgaggac acggggagtt ggagcgcgct gcgccccgtc gcgacggagc
     1201 gttccgcgcg cgatccggat cctgatggtt cggaggaccg gcccgggggt aggaacgacg
     1261 cctggtcggc cgagcctgag tacacgcggc ggacgacgcc gggccgccca ggcgccgggt
     1321 ggccgctccc ggaggggagc gctccggagg cggcggatga cggggcgggg gcagccgacc
     1381 ccgaggagga ggccgacagg ctcggaccga ggagccctgc cagcgacatc tccgtgttca
     1441 ccacctcgac ccgctgggtc ccgtcgccgc acgacggcta tgtttccgac gacgtttccg
     1501 acgacgcgcc gggcgacgag gggtaccctg cggccgccga cccagaccgt ggttggagcg
     1561 gcggcgggcc gccgtcggcg ggtcggggca ccggcgtcgc tcgcacgtcc agcaacgcgt
     1621 acaacggcgc ccagtggatc caaccccccg cgagggcaga agctccccgc ctgcgcccgt
     1681 atctcgtctg gagtctcatc gcctcggtgg cggtcttcct gttgctgcag tacgtgatgg
     1741 ccggccgtat ggaaggagcg cccgcgctcg gtaccttcga actcggcatg aacgcctacc
     1801 gtagcggcga cttcggcgcc gccaggcgct cgctcgagcc ggaggccgaa gccggcaacc
     1861 acctcgccaa cgacgccaac gagaacggtg tcaccgattc cg
//
LOCUS       DAOQYE010000436         1901 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_71755_length_1901_cov_3.044420, whole genome shotgun sequence.
ACCESSION   DAOQYE010000436 DAOQYE010000000
VERSION     DAOQYE010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71755_length_1901_cov_3.044420"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="PKN52_07505"
     CDS             complement(<1..298)
                     /locus_tag="PKN52_07505"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNQ99832.1"
                     /translation="MSPQPGDQGFDTRSVTADLRLAGAGDLGRLFREARESKGLELGD
                     VAELTHVRREYLRALEGGRFADLPEDVYTRNFVRLFAHAVGLPADEAMERYQRER"
     gene            complement(295..1233)
                     /locus_tag="PKN52_07510"
     CDS             complement(295..1233)
                     /locus_tag="PKN52_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HNQ99833.1"
                     /translation="MSLLASLRISAEVADALANRAPVVALESTVITHGLPRPHNLELA
                     TRLEEVVREAGAVPATVGVVAGEVRVGLERSDLERLAAGGADKASLWNLAALVAAGRD
                     AGTTVATTLYAAHGAGIKVFATGGIGGVHDTPFDESADLGALAAYRVLTVCAGPKSIL
                     DAEATTERLETLGVPMVGYRSDRLAGFVVRETSIRLPSRVDDPAAAAAVLRAQEQLGL
                     RSGVLVSNPVSAGLSHDRFDAWLQAARASAAVAGVHGRDTTPYLLDAIAKLSGGETMT
                     VNLRLLEENARLAATIAGAYAMTGANTRALAAVGEP"
     gene            1303..1629
                     /locus_tag="PKN52_07515"
     CDS             1303..1629
                     /locus_tag="PKN52_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUN14 domain-containing protein"
                     /protein_id="HNQ99834.1"
                     /translation="MSVDLSSVMPWVQQLSFGAVAGFVAGFALKKVGKFVALVIGVLF
                     VVIQLLAWSGYLSVDWGRIQESVDPLLEPTRLEQLWQGLLAMLTYNLPFGAAFVTALV
                     IGLRRG"
     gene            1782..>1901
                     /locus_tag="PKN52_07520"
     CDS             1782..>1901
                     /locus_tag="PKN52_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99835.1"
                     /translation="MADKKQTLRPIGDKVVVEVIDEPQTTLSGIVLPDSAKEKS"
BASE COUNT          287 a          624 c          680 g          310 t
ORIGIN      
        1 ggcgttctcg ctggtagcgc tccatcgcct cgtcggccgg gaggccgacg gcgtgcgcga
       61 agagcctgac gaagttgcgg gtgtagacgt cctcgggcag gtccgcgaag cggcctccct
      121 cgagcgcgcg caggtactcg cgcctgacgt gcgtgagttc ggccacgtcg ccgagctcga
      181 gacccttcga ctccctggct tccctgaaga ggcgtccgag gtcgccggcg ccggccaacc
      241 gcaggtccgc ggtcaccgaa cgcgtgtcga agccttgatc gcccggctgc gggctcatgg
      301 ctcacccacc gcggcaagcg cgcgcgtgtt cgcgccggtc atggcgtagg cgcccgctat
      361 ggtggcagcc agcctggcgt tctcctccaa caggcggagg ttgacggtca tcgtctcccc
      421 gccggagagc ttggcgatgg cgtcgagcag gtagggcgtc gtgtcgcgac cgtgcacgcc
      481 ggcaaccgcc gcgctcgccc gggcggcctg gagccaagcg tcgaagcggt catgggagag
      541 gccggcgctg accgggttgc tgacgagcac cccgctgcgc agaccgagct gctcctgggc
      601 gcgcagcacg gcggccgcgg cggccgggtc gtcgacgcga gaggggaggc ggatggacgt
      661 ctccctgacg acgaagccgg ccagccggtc ggagcggtac ccgaccatcg gaacgccgag
      721 ggtctcgagg cgttcagtgg tcgcctcggc gtcgaggatg ctcttggggc cggcgcacac
      781 ggtcagcaca cggtaggcgg ccagcgcgcc gaggtcggcg gactcgtcga acggcgtgtc
      841 gtgcacgccg cctatgccgc ccgtggcgaa caccttgatg cccgctccat gcgcggcgta
      901 gagggtagtg gctacggtcg taccggcgtc gcggccggcg gcgaccaggg cggcgaggtt
      961 ccagagggag gccttgtccg cgccgccggc cgccagtcgc tcaaggtcgg accgttcgag
     1021 gccgaccctc acctcgccgg ccactacgcc gacggtggcg ggcacggctc cagcctcgcg
     1081 cacgacctct tcaaggcgcg tggcgagttc gaggttgtgt ggccggggca aaccgtgcgt
     1141 gatgacggtc gactccagcg ccaccaccgg cgcgcggttg gccagcgcgt ccgctacctc
     1201 ggccgagatg cgcagcgaag cgaggaggct cacgaggtcg atattacccc agccgcagga
     1261 gcgctgctgg ctctggtcgg gccgtgctac ccttctttcg gagtgagcgt cgacctctcc
     1321 agcgtcatgc cttgggtgca acaactgtcc ttcggagcgg tggccgggtt cgtggcgggc
     1381 ttcgccctca agaaggtcgg caagttcgtc gccttggtga taggcgtcct cttcgtggtc
     1441 atccaactcc tggcatggtc gggctacctt agcgtcgact ggggtcgcat ccaggagtcg
     1501 gtcgacccgc tgctcgagcc gaccaggctg gagcagttgt ggcagggcct actggccatg
     1561 ctcacgtaca acctaccgtt cggggccgcc ttcgtcacgg cgctcgtgat cggtctccgc
     1621 cgcggttgac ctgcggcctg atgagcaagt gacatgagtg acatggactc agatggcttg
     1681 atatatccgg cttgacatcc tctatgggcg gtgggtagga tatggccgtt tggcactctc
     1741 atggtgagac tgccaagttc agcccttcaa cggaggaacc catggcggac aagaagcaga
     1801 ccctcaggcc gatcggcgac aaggtcgtgg tcgaagtcat cgacgagccc cagacgaccc
     1861 taagcggcat cgtgttgccg gacagcgcca aggagaagag c
//
LOCUS       DAOQYE010000437         1899 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_71893_length_1899_cov_2.302061, whole genome shotgun sequence.
ACCESSION   DAOQYE010000437 DAOQYE010000000
VERSION     DAOQYE010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71893_length_1899_cov_2.302061"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="PKN52_07525"
     CDS             complement(<1..309)
                     /locus_tag="PKN52_07525"
                     /inference="COORDINATES: protein motif:HMM:NF019533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HNQ99836.1"
                     /translation="MTDPAALTRLEGWRAEKDAFLRDHPRSPLSGAAGFGGLAYYPYD
                     PGALVVARLERAERPARVFMATSSGEERAYLEFGVASFRLYGADAALTLLASVAEMET
                     L"
     gene            complement(311..1366)
                     /locus_tag="PKN52_07530"
     CDS             complement(311..1366)
                     /locus_tag="PKN52_07530"
                     /inference="COORDINATES: protein motif:HMM:NF014447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99837.1"
                     /translation="MTTDVGHEPVALSRVHRPRVLLPWRASSFPADWDEVFGRSPCPV
                     GLTVEVGFGDGRFTVARALAEPAMRFVGLEVSSGSLQRALKRIERSGASNVRLAKAGA
                     HVALQQLFAPASLDALVVNFPCPWPKEKHAKHRLLSRRFLALAGSRLKAAGPADAGLD
                     GAGEVRLATDHPEYLGYALEEAAATGLYDVLRPEAPEGVFETKYALKWRDQGKPLHYV
                     VFRRNAVEAPEHPHLERPHTMPHALLRGQLPLEQPFTKVVLPYADGHVILHEAMRSLG
                     GEDGRGRVLVRATVEEDAMRQQLLVVAQQRGVDEVIVRLETFGDPLVTPTARGCVHAV
                     TEWLLASGELSVKARNY"
     gene            complement(1366..>1899)
                     /locus_tag="PKN52_07535"
     CDS             complement(1366..>1899)
                     /locus_tag="PKN52_07535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99838.1"
                     /translation="EERRSTGREEVPTLSRDAMPALPVVGPYGDRAVTLTAEEQRVLA
                     HVDGHRTVTRIAVDLGIEPEEALVICDRLIRTGVLKLQNRRARTARLVTRLATRRQAA
                     GSVGLDPTIVEAWEKVLGHSVTKVVCRRDDGSVQLFPAFAAKGAGPYLELSRDTLVRA
                     DLRVNEALLVRPYVEDA"
BASE COUNT          274 a          656 c          701 g          268 t
ORIGIN      
        1 cagcgtttcc atttcggcga cggaggcgag cagggtcaga gcggcgtcgg ccccgtacag
       61 acgaaagctc gcgacgccga actccaggta ggcgcgctcc tctccgctgc tcgtagccat
      121 gaacacccgt gccggtcgct cggctcgctc caaccgcgcg accaccagcg cccccggatc
      181 gtacgggtag taggcgaggc cgccgaaacc ggctgcgccg ctcaacggcg agcgcgggtg
      241 gtcgcgcagg aacgcgtcct tctcggcccg ccagccctcc aaccgcgtga gcgccgccgg
      301 atcggtcatc tcagtagttc ctggccttca cgctcagctc accggaggcg agcagccatt
      361 cggtgacggc gtggacgcag ccccgcgccg tcggggtcac gagcgggtcg ccgaaggtct
      421 ccaggcgcac gatgacctcg tcgacgccgc gctgctgggc cacaaccagg agctgctgtc
      481 gcatggcgtc ctcttcgacg gtggcgcgaa ccaggacacg tccgcggccg tcctcgccgc
      541 cgagcgagcg catggcctcg tgcaggatga cgtgcccgtc agcgtacggg agcacgacct
      601 tggtgaacgg ctgttcgagc gggagctggc cccgcaggag cgcgtgtggc atcgtgtgtg
      661 gcctctctag gtgggggtgt tctggcgcct cgacggcgtt gcgcctgaac accacgtagt
      721 gcagtggctt gccctggtct cgccatttga gggcgtactt ggtctcgaag acgccctcgg
      781 gggcctcggg ccggaggaca tcgtagagcc cggtggcggc ggcttcctcg agcgcgtagc
      841 ccaggtactc ggggtggtcg gtcgcgagcc gcacctcgcc ggcgccgtcg agaccggcgt
      901 cggcgggacc cgcggccttg aggcgcgagc cggccagcgc gagaaagcgg cggctgagca
      961 ggcgatgctt ggcgtgcttc tccttcggcc acgggcacgg gaagttgacg accagcgcgt
     1021 cgagggacgc gggggcgaag agctgctgga gcgcgacgtg cgcgccggcc ttggccagcc
     1081 gcacgttgct agcgccggag cgctctatgc gcttgagggc ccgctgcaag ctgccggagg
     1141 agacctcgag gccgacgaag cgcatcgcgg gctcggcgag cgcgcgggcc acggtgaagc
     1201 gaccgtcgcc gaagccgacc tcgacggtca ggccgaccgg gcaggggctt cggccgaaca
     1261 cctcgtccca atccgctggg aagctcgagg cgcgccatgg gaggagcacg cgcggccggt
     1321 ggacacgcga caacgccaca ggctcatggc ccacgtccgt cgtcatcagg cgtcctcgac
     1381 gtacggtctg acgagcaagg cctcgttgac acgcaggtcg gcccgcacga gggtgtcccg
     1441 gctgagctct aggtaggggc cggcgccctt ggcggcgaac gccgggaaga gctgcacgct
     1501 gccatcatcg cgccggcaga cgaccttggt gacggagtgc ccgaggacct tctcccacgc
     1561 ctccacgatc gtcggatcga ggccgacgct gccggccgcc tggcgccggg tcgcgagtct
     1621 ggtcacgagc cgggccgtgc gcgcgcggcg gttctgcaac ttgaggacgc cggtgcggat
     1681 gaggcggtcg cagatcacca acgcctcctc cggctcgatc ccgaggtcga ccgcgatgcg
     1741 cgtcaccgtc ctatgcccgt ccacgtgagc caggacgcgc tgctcctccg cggtgagggt
     1801 gacggcccgg tcaccgtacg ggccgacgac gggcagggcg ggcatggcgt cgcgggagag
     1861 ggtgggcacc tcctcgcggc cggtggagcg ccgctcctc
//
LOCUS       DAOQYE010000438         1894 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_72204_length_1894_cov_2.742251, whole genome shotgun sequence.
ACCESSION   DAOQYE010000438 DAOQYE010000000
VERSION     DAOQYE010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72204_length_1894_cov_2.742251"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..921)
                     /locus_tag="PKN52_07540"
     CDS             complement(<1..921)
                     /locus_tag="PKN52_07540"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNQ99839.1"
                     /translation="MTDRPGRRSDGSRPRTVTNADVAAAAGVSTMTVSRVVNGTGRVS
                     EATRERVRRAIEELGYQPNQLARSLTQGRTRTIGIVVPDITNPFFPEIVRGAEDAAWA
                     AGYTVALANAVEDQARERAALRNLAGHRVDGVIVCSARLPDHELTDLLSAHPASVLIN
                     RKVPGAPAVSLMVDDLLGAHMAVGHLLERGRKNLALLGGPDRSASARYRREGFLAAMR
                     EAGVSVSDALLTHCEPVEDAGYEAASRWVAQANAGGPRFDGIVAYNDLAALGAVRALR
                     AAGLRVPEDVAVVGCDDIRMASAVAPSLTTL"
     gene            complement(929..1747)
                     /locus_tag="PKN52_07545"
     CDS             complement(929..1747)
                     /locus_tag="PKN52_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNQ99840.1"
                     /translation="MSRDRFDLTGRVALVTGATGVLGGEMARGLAAHGAKVAVLGRNA
                     ERAEELAAGLASEGYEALATPADVLDRAALERARAALLERFGRVDILVNAAGGNMPGA
                     TLAPGASFLDLGEEPMRGVIDLNYMGTVLPIQVFGAELAKGGCGSIVNISSMAALRAI
                     TRVVGYSAAKAAIDNLTRWLAMEFGRQYGGGLRVNAMAPGFFIGNQNRRLLLEEDGSL
                     TARGRTILDHTPLGRFGEPEELVGTLVWLCSDASRFVTGVVVPVDGGFAVTSGV"
BASE COUNT          236 a          694 c          716 g          248 t
ORIGIN      
        1 cagcgtcgtc aacgacggcg cgacggcgct ggccatgcgg atgtcgtcgc agccgaccac
       61 cgccacgtcc tcgggcacgc gcaggccggc cgcgcgcagg gcgcgcaccg cgccgagtgc
      121 cgccaggtcg ttgtaggcga cgatgccgtc gaaccttggg ccgccagcgt tcgcctgcgc
      181 cacccagcgg gacgcggcct cgtagccggc gtcctcgacg ggctcacagt gcgtcaggag
      241 cgcgtcgctc acgctcacgc ccgcctcgcg catggcggcg aggaacccct cgcgccggta
      301 cctggcgctg gccgagcggt ccgggccgcc gaggagcgca aggttcttgc ggccgcgttc
      361 cagcaggtgc ccgacggcca tgtgggcgcc gaggaggtcg tcgaccatga ggctcacggc
      421 cggcgccccc ggcaccttgc ggttgatgag cacggaggcc gggtgggccg agagcaggtc
      481 ggtcagctcg tggtccggga ggcgcgcgct gcagacgatc accccgtcga cccggtgccc
      541 ggcgaggttg cgcagggcgg cgcgctcgcg cgcctggtcc tcgacggcgt tggcgagcgc
      601 caccgtgtag ccggcggccc aggcggcgtc ctcggctccg cgaacgatct ctgggaagaa
      661 ggggttcgtg atgtcgggca cgacgatgcc gatggtgcgc gtgcggccct gcgtgaggga
      721 gcgcgcgagc tggttcggct ggtagccgag ctcctcgatg gcccggcgga cgcgctcgcg
      781 cgtcgcctcg ctgacgcggc ccgtgccgtt cacgacgcgc gagactgtca tagtcgacac
      841 gcccgcggcc gccgcgacgt cggcgttcgt gacggtcctc ggcctggagc cgtcgctgcg
      901 tcgcccgggc cggtcggtca cgtccgggtc agaccccgct ggtgacggcg aagccgccgt
      961 ccacgggcac cacgacgccc gtcacgaagc ggctggcgtc cgagcagagc catacgagcg
     1021 tgccgacgag ctcctccggt tcgccgaagc gcccgagggg cgtgtggtcg aggatggtcc
     1081 ggccgcgcgc cgtgagcgac ccgtcctcct cgagcaggag ccggcggttc tggttgccga
     1141 tgaagaaccc gggcgccatg gcgttgacgc gcaggccgcc gccgtactgg cggccgaact
     1201 ccatggcgag ccagcgcgtc aggttgtcga tggcggcctt cgccgccgag tagccgacga
     1261 cgcgcgtgat ggcccgcaac gccgccatcg aggagatgtt cacgatgctc ccgcagccgc
     1321 ccttggcgag ctccgccccg aacacctgga tgggcaggac ggtgcccatg tagttgaggt
     1381 cgatcacacc gcgcatgggc tcctcgccca ggtcgaggaa gctggcgcct ggcgccaggg
     1441 tggcgccggg catgttgccg ccggccgcgt tcacgaggat gtcgacgcgg ccgaagcgct
     1501 ccaggagcgc cgcgcgcgcg cgttcgaggg ccgcgcggtc gaggacgtcg gccggcgtgg
     1561 ccagcgcctc gtacccttcg gaggcgaggc cggcggccag ctcctcggcg cgctcggcgt
     1621 tcctgcccag cacggcgacc ttcgccccgt gcgcggccag gccgcgcgcc atctcgccgc
     1681 ccaggacgcc cgtggcgccc gtgaccagcg ccacgcggcc ggtgaggtcg aagcggtcgc
     1741 ggctcataga tgagccgttg ctcatcgcag cgccgccgct ttcagaggag ccttggcccg
     1801 ccgaggcggc gccactcgcg gccccgccgc tcatcgcagc cccgccgcgg acgccgggtc
     1861 caggtagagc cgcgcgtgct gcacggtgcg cagg
//
LOCUS       DAOQYE010000439         1890 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_72434_length_1890_cov_3.307902, whole genome shotgun sequence.
ACCESSION   DAOQYE010000439 DAOQYE010000000
VERSION     DAOQYE010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72434_length_1890_cov_3.307902"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1483)
                     /locus_tag="PKN52_07550"
     CDS             complement(<1..1483)
                     /locus_tag="PKN52_07550"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HNQ99841.1"
                     /translation="MSLLDGRYEVIAQRALDSWRTEFEATAPDGTPLRIEWFDLKPER
                     EPEFEAYRRLLRRLKAAGLAAVHDVIARPGAHYVAWERPPADSAAVSPAAGNGAATEL
                     RELLQRAGYALESCDMRRQHARQPRTLLYGLPFGAPRRAPQPQGPHVGREPTLASDPP
                     ASYGPAGSSDAVGPHGSGAARGASANTDLAGPLAARPLRHQQSLGRRAAELLERLPER
                     TLSWGVAGLLTLVAVVAALVAVKLAAVDAVVTVPEVIGADATLAAQQLVQLGLVVDAT
                     PLTSSEPPGTVLAIDPPTGSALRPGRTVLMRYALPSGELAPTETPSLLGLSYPEGARA
                     ALEAAGLRLGDAAHIHAPSPAGTVLAQDIDAGNRVANDTAVSVLVSVGPRVPQTFVPN
                     LVGLDLDEAKTLARLAGISEDRVVVDELLATAGANGQVLEQSLAPYVPVARDTAVLRL
                     VVQAGEPAGPAQTGGAPDVVGLDLAAAREVASGWDLEVRVLGNP"
     gene            complement(1544..>1890)
                     /locus_tag="PKN52_07555"
     CDS             complement(1544..>1890)
                     /locus_tag="PKN52_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein FtsH"
                     /protein_id="HNQ99842.1"
                     /translation="EVSSILEAAYRESEAVIANNWVMVEAVATALLKRETLDGKLIHR
                     AFDLAQEGKTAAEVADWIVDTATENDRRAQAAEERERRAAEEEHRRKAAEGGRPPLEP
                     RPEARPAGESGD"
BASE COUNT          242 a          681 c          708 g          259 t
ORIGIN      
        1 ccgggttgcc gagcacgcga acttccaggt cccacccgct cgctacctcg cgcgcggccg
       61 ccaggtcgag gccgacgacg tcgggcgccc cgcccgtctg cgcagggccg gcgggctcgc
      121 cggcctggac gacgaggcgc aggaccgccg tgtcgcgcgc caccggcacg tagggtgcga
      181 gagactgctc caacacctgg ccgttggcac cggccgtcgc aagtagctca tcgaccacta
      241 cccgatcctc gctgatcccg gccaggcgcg ccaacgtctt ggcctcgtcg aggtcgaggc
      301 cgacgaggtt ggggacgaag gtctgcggga cacggggccc gacgctcacc agcacggaga
      361 cggccgtgtc gttcgccacc cggttgccgg cgtcgatgtc ttgcgcgagc acggtgcctg
      421 ccgggctcgg agcgtggatg tgggcggcgt ccccgagccg caggcccgcg gcctcgagcg
      481 cggcgcgcgc gccctccggg taactcaggc ccaggagcga gggcgtttcc gtaggcgcca
      541 gctccccaga ggggagggca tagcgcatga gcaccgtgcg gcccggtctg agggccgagc
      601 cggtcggcgg gtcgatggcg aggaccgtgc cggggggctc agaggacgtg agcggcgtcg
      661 cgtcgaccac gaggccgagc tgcacgagct gctgcgccgc gagggtggcg tcggcgccga
      721 tcacctcggg tacggtaacg acggcgtcga cggcggccag cttgacggcc acgagggccg
      781 ccactacggc cacgagggta agcaggcctg cgacgcccca ggagagcgtc cgttcgggta
      841 gccgctcgag cagttccgcc gctcgtcggc cgagcgactg ctgatggcgg agcggtcggg
      901 ccgcgagcgg cccggcgaga tcggtgttag cggacgcacc gcgcgccgcg ccagagccat
      961 gcggtccgac cgcgtcggaa gacccggccg ggccgtaaga cgcgggcggg tcggaagcca
     1021 gggtgggctc ccgacccacg tgaggtcctt gcggctgggg cgcccgccgg ggcgctccga
     1081 agggcaatcc gtataggagc gtgcggggtt ggcgtgcgtg ctgacgccgc atgtcgcagc
     1141 tctccaaggc gtaaccggcg cgctgcagca actcgcgcag ctccgtcgcg gcgccgttgc
     1201 cggccgccgg ggagacggcg gccgagtcgg ccggcggcct ctcccaggcc acgtaatggg
     1261 cgcctgggcg cgctatcaca tcgtggacgg ccgccaaccc ggcggccttg aggcgccgca
     1321 acaagcgccg ataggcctcg aactccggct cgcgctccgg cttcagatcg aaccattcga
     1381 tgcgcagcgg cgtgccgtcg ggcgccgtgg cctcgaactc cgtgcgccag gagtcgagcg
     1441 cgcgttgcgc tatgacctcg tacctgccgt cgagtaacga cattcaagcc cccgccgtac
     1501 gcgtcggcct aacgctcgtc ggcatggcgc cgcaagcgcc gggtcagtcc ccggactcgc
     1561 cggccggtcg cgcctctggc cgcggctcga gcggcggtct gccgccctcg gccgccttgc
     1621 ggcggtgctc ctcctcggcc gccctgcgct cgcgctcctc ggccgcctgc gcgcgtcggt
     1681 cgttctcggt ggccgtgtcg acgatccagt cggcgacctc ggcggcggtc ttgccctcct
     1741 gggcgaggtc gaacgcccgg tggatgagct tgccatcgag ggtctcgcgc ttgaggagcg
     1801 ccgtggcgac cgcctcgacc atgacccagt tgttcgcgat cacggcctcg ctctcgcggt
     1861 aggcggcctc gaggatgctg gagacctcct
//
LOCUS       DAOQYE010000440         1889 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_72511_length_1889_cov_2.966194, whole genome shotgun sequence.
ACCESSION   DAOQYE010000440 DAOQYE010000000
VERSION     DAOQYE010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72511_length_1889_cov_2.966194"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(181..780)
                     /locus_tag="PKN52_07560"
     CDS             complement(181..780)
                     /locus_tag="PKN52_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99843.1"
                     /translation="MGREARAIAAWRGQTAQVKALLESHEIVLRGGIRARIPRSDVTA
                     VATVGGRLDLLAAGEPLVLTLGEPEAGKWAAALLRPPPSLADKLGVSTSKRAFVTGSL
                     ADGTLRAALAGATTEALSDATLIVAVLERAADLDAAFAVAESAPHLAIWCVYPKGRGS
                     SVGDSQVRNYLRERGYIDSKTSAVSDRLTATRYVLRKNT"
     gene            complement(770..1420)
                     /locus_tag="PKN52_07565"
     CDS             complement(770..1420)
                     /locus_tag="PKN52_07565"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNQ99844.1"
                     /translation="MRIAGLSEASGVSISTLKYYLREGLLHPGDARAVNQAEYGEGHV
                     RRVRLIRALLELGKLQLADIRRVLAAVDDEGVAIHDAFGVAQDAMVPARDRGSPEYGR
                     AFVAVEEFVRRHGMRVRQDAQVRGMLADALVAMWSGGWDLDLRTFDASMPRILAEAGQ
                     ELEGVPDDDRAVQMEVTVIGTIAYEVALAALRRMALEHASAERFALSALPGSAHGT"
     gene            1508..>1889
                     /locus_tag="PKN52_07570"
     CDS             1508..>1889
                     /locus_tag="PKN52_07570"
                     /inference="COORDINATES: protein motif:HMM:NF025196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4188 domain-containing protein"
                     /protein_id="HNQ99845.1"
                     /translation="MRAVPGRYTASIEGDFVVFVIGMRINKLWKPHKWVPVMSAMGPM
                     LRELFTHPEKGLLSAQFGWSGRTITLIQYWRSFEQLERFARDRNDPHLPAWRRFNRRV
                     GTGGDVGIFHETFKVAAGAYETVYN"
BASE COUNT          329 a          648 c          613 g          299 t
ORIGIN      
        1 gagcgcgacc tcgaccgcgg ctccgtcgct cagtacctgc acgctgttca tggcggcaag
       61 atacgcttga caagaaaaat tgtcaaggca gagcgtcgtg aaccggtacg gacaccggag
      121 gccgagggtc ggcggcaacc ggtctcaggt ttcgaccgac ccttcgggta aggcggcgcg
      181 tcaggtgttc ttgcgcagaa cgtaccgtgt cgcggtgagc cggtcggaga ccgcgctggt
      241 cttggagtcg atgtagccgc gctcacgcaa gtagttccgc acctgggagt cgcctaccga
      301 gctgcccctg cctttcgggt agacgcacca tatggcgagg tgcggcgcgg attccgcgac
      361 cgcgaaggcc gcgtcgaggt cggccgcccg ttccagcacg gccacgatga gcgtggcgtc
      421 gctcagcgcc tcggtggtag cgcctgccag cgcggcgcgc aaagttccgt cagccagcga
      481 ccccgtcacg aaggcccgct tcgaagtgga gacgcctaac ttgtcggcga ggctgggcgg
      541 gggcctgagc aaggcggcag cccacttgcc agcctcaggt tcgccaagcg tcaggacgag
      601 cggctcgccg gcagcgagca agtcgagacg gcctcccact gtggccaccg cggtgacatc
      661 ggagcggggg atgcgcgctc ggatacctcc gcgcaggacg atctcgtggg actctagcag
      721 cgccttgact tgcgccgtct ggccacgcca ggccgcgatc gcgcgagcct cacgtcccat
      781 gagcagaccc cggaagcgca ctcagcgcga accgctccgc gctcgcgtgc tctagagcca
      841 tccggcggag cgcggcgagg gccacctcgt aggcgatggt cccgatcacc gtcacctcca
      901 tctgcaccgc gcggtcatcg tcgggcactc cctccaactc ttgtcccgcc tcggcgagga
      961 tcctcggcat gctcgcgtcg aaggtgcgca ggtcgaggtc ccagcccccg gaccacatgg
     1021 caacgagcgc gtcggctagc atccctcgga cctgagcgtc ttggcgcacc cgcatgccgt
     1081 gccgccgcac gaactcctcc accgccacga aggctcgccc gtactcgggg gagccccggt
     1141 cccgagccgg gaccatggcg tcctgagcca ccccgaaggc gtcgtggatc gcgacgcctt
     1201 cgtcatcgac ggcggcgagc acgcgccgga tgtcggccaa ctgcagcttg ccgagttcca
     1261 gcagcgcccg gatcagccgc acacgccgaa catggccctc gccgtactcc gcctggttca
     1321 ccgcgcgcgc atcgcctggg tgaagcagcc cctcgcgtag gtagtacttg agggtcgaga
     1381 tactgacccc gctcgcctcg ctgagcccgg ctatccgcat tcgtgcccgc agtcgatgct
     1441 tgacatctcc catagatact ggcactatct attatggata gtcaagctat ccattcagga
     1501 ggccagcatg agagccgtcc cgggccggta cacagcgagc atagaaggcg acttcgtcgt
     1561 cttcgtgata ggcatgcgca tcaacaagct ctggaagccg cacaagtggg tgccggtcat
     1621 gagcgcgatg ggacccatgt tgagggagct gttcacccac cccgagaaag ggttgctgag
     1681 cgcccagttc ggttggtcgg ggcgcacgat cacgctcatc cagtactggc ggagcttcga
     1741 gcagctggaa cggttcgcgc gcgaccgcaa cgacccccac ctaccggcat ggcggcgctt
     1801 caaccgaagg gtcggcacgg gcggcgacgt gggcatcttc cacgagacct tcaaggtcgc
     1861 cgcaggcgca tatgagaccg tgtacaaca
//
LOCUS       DAOQYE010000441         1885 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_72755_length_1885_cov_2.860656, whole genome shotgun sequence.
ACCESSION   DAOQYE010000441 DAOQYE010000000
VERSION     DAOQYE010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72755_length_1885_cov_2.860656"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            73..222
                     /locus_tag="PKN52_07575"
     CDS             73..222
                     /locus_tag="PKN52_07575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99846.1"
                     /translation="MTAKARDGADCVLTEDGEPESQKVEAGQVAEQKRARTLAEAERR
                     ARLAS"
     gene            250..1479
                     /locus_tag="PKN52_07580"
     CDS             250..1479
                     /locus_tag="PKN52_07580"
                     /inference="COORDINATES: protein motif:HMM:TIGR01554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HNQ99847.1"
                     /translation="MSRVLTLKKQYRDLVGEAKAIRDKYTNTAENMTDEEAATFDSIM
                     EKADRVWSEVEREERAEKSFKMVDEAEEHVHGSGDDEDTKRKKISTLQKKAFDAFLIG
                     GSTDPDYVSKQIELKVLQADLDTSGGYLVAPQAFVQQLLKAVDDQVMIRQYATVYPLG
                     ANESLGVPSLDTDLNDADWTTELATGNQDDATRFGKRELRPHPFAKRVKISNKLLRSA
                     LMDPEAHVRERLAYKFGITEEKAFMTGNGAEKPLGLFTASAQGISTGRDVTIGTTTAV
                     TADGFIDVLHGLKPQYWNRSRWLMHRDVLKQVRKLKDGNGQYLWQAGLAGGTPNTILD
                     RPYMISEFAPNTLTTGQYVALIGDFTFYWIVDALGLTVQRVVELYAESNATGFIGRAE
                     CDGMPVLEEAFSRGKLA"
     gene            1540..>1885
                     /locus_tag="PKN52_07585"
     CDS             1540..>1885
                     /locus_tag="PKN52_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99848.1"
                     /translation="MQNLTNKIQTIIAHAPVAAGTSNITDCVVIDTQGYEGLRFIVGF
                     GTITSSAVTSLKAQQSEVKASATSLTGGADLAGTGITVADTDDNKIFIMDIYRPTKRY
                     VQLFINRGTANAV"
BASE COUNT          404 a          633 c          556 g          292 t
ORIGIN      
        1 ccttgatcat gaatgcgcgg atcgccgccg ggtggatcga agcgccttcc gaggaagtcg
       61 aggaggcgtc cgatgacggc gaaggctcgt gacggcgccg actgcgtact gaccgaagac
      121 ggcgagccgg agtcccagaa agttgaggcc ggccaggtcg ctgagcagaa gcgggcgcgt
      181 acgctcgccg aggccgagcg ccgggcgcga ctcgccagct agactcactc aacaggagcg
      241 agaaaagaaa tgagcagggt tctcaccctc aagaagcagt accgtgacct cgtcggcgag
      301 gctaaggcca tccgcgacaa gtacacgaac accgccgaga acatgaccga tgaggaggcc
      361 gcgaccttcg actcgatcat ggagaaggca gaccgcgtct ggtctgaggt cgagcgcgag
      421 gagcgcgccg agaagtcgtt caagatggtg gacgaggctg aagagcacgt tcacggctcg
      481 ggcgacgacg aggacaccaa gcgcaagaag atctcaaccc tccagaagaa ggctttcgac
      541 gcgttcctca tcggtggtag caccgacccg gactacgtct cgaagcagat cgagctcaag
      601 gtgctccagg ctgaccttga caccagcggc ggttacctcg tggccccgca ggcgttcgtc
      661 cagcagcttc tcaaggctgt cgacgaccag gtcatgatcc gccagtacgc gaccgtctac
      721 ccgctcggcg ccaacgagtc gcttggtgtt ccgtcactgg acaccgacct caacgacgct
      781 gactggacga ccgagctcgc gaccggcaac caggatgacg ccacccgctt cggaaagcgc
      841 gagcttcgcc cgcacccgtt cgcgaagcgc gtgaagatct cgaacaagct tctccgctcg
      901 gccctcatgg acccggaggc tcacgttcgt gagcgtctcg cctacaagtt cggtatcacc
      961 gaagagaagg cgttcatgac cggcaacggc gccgagaagc ctcttgggct cttcaccgcg
     1021 tcggcgcagg gcatcagcac cggacgcgac gtcaccatcg gcacgaccac cgcggtcacc
     1081 gctgacggtt tcatcgacgt gctacacggc ctcaagccgc agtactggaa ccgctcgcgc
     1141 tggctcatgc accgcgacgt tctcaagcag gtccgcaagc tcaaggacgg aaacggccag
     1201 tacctctggc aggccggcct agcgggcgga accccgaaca ccatcctcga ccggccgtac
     1261 atgatcagcg agttcgctcc gaacaccctg accaccggcc agtacgtggc tctcatcgga
     1321 gacttcacct tctactggat cgttgacgcc ctcggcctca ccgttcagcg cgttgttgag
     1381 ctctacgctg agtcgaacgc caccggcttc atcggccggg ccgagtgcga cggcatgccc
     1441 gtcctcgagg aagcgttcag ccgcggcaag ctcgcctaaa acacctgagg agccgagcgg
     1501 aggctcctct accagctttt acgggaaggg aaaaacaccg tgcagaacct caccaacaag
     1561 atccagacca tcatcgccca cgctcctgtc gcggccggca cctcaaacat cactgactgt
     1621 gtcgtcatcg acacccaggg gtacgagggt ctccgcttca tcgtcggctt cggcaccatc
     1681 acctcgtcag ctgtcacctc gttgaaggcc cagcagtcgg aagtcaaggc ctcagccacc
     1741 tccctcaccg gcggtgctga cctcgcggga acgggcatca ccgttgccga cacggacgac
     1801 aacaagatct tcatcatgga catctaccga ccgaccaagc gctacgtgca gttgttcatc
     1861 aaccgcggaa ccgcgaacgc cgtcg
//
LOCUS       DAOQYE010000442         1883 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_72901_length_1883_cov_2.260394, whole genome shotgun sequence.
ACCESSION   DAOQYE010000442 DAOQYE010000000
VERSION     DAOQYE010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1883
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72901_length_1883_cov_2.260394"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="PKN52_07590"
     CDS             complement(<1..200)
                     /locus_tag="PKN52_07590"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNQ99849.1"
                     /translation="MIEVSNLSKVFPDGTQALSEVNVTVPDGDFIAVIGLSGAGKSTF
                     LRCLNRLVDPSEGSITVGSKDVA"
     gene            complement(285..1175)
                     /locus_tag="PKN52_07595"
     CDS             complement(285..1175)
                     /locus_tag="PKN52_07595"
                     /inference="COORDINATES: protein motif:HMM:NF024376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HNQ99850.1"
                     /translation="MKEALVKFSLLVAAIATLFSFAAAQAPITVRIGFNPTQNSDLLT
                     SASEGIANYLESQLGGAVEVQVFIPTDYRGLIEAMGSGNLDFAFFPPDGFVVANSEVG
                     AQVLLKSVRTGNPYYWSAIVVRKDSGIENIGDLEGKSIAWIDPNSAAGYTFPRAELIT
                     AGINPDDFFSNQVYAGGHDAAVLAVLNGSVDAAATFANDDQNISGAWTQFLDADQASQ
                     ITAIFYTRPIPGDTFSVSQQFASEHAALTYQIAAAIATIKAPDNNLLMDLYRIDYMIP
                     ADSSDYAVVRDTRRILGLDQ"
     gene            complement(1245..>1883)
                     /locus_tag="PKN52_07600"
     CDS             complement(1245..>1883)
                     /locus_tag="PKN52_07600"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNQ99851.1"
                     /translation="RILVVDDHELFLEGLTLLVSTMPGCEVVGMAHDGAQAVDLARRL
                     APRVVLMDARMPNLSGPEAIASLVADLPGTAVVMVTMFADDDLVFQAMRAGAKGYVLK
                     GASKDELQRAIMAAANGEALFDHDIVERFARYFSVGQTGGPLAFGLTPREREVLELLA
                     RGLHNHEMAKELRVTAKTVRNHVSNVISKLGVTSRYEAATKAREAGLLRGRA"
BASE COUNT          306 a          579 c          672 g          326 t
ORIGIN      
        1 gcgacgtcct tcgagcccac cgtgatggag ccctcgctag ggtcgaccaa gcggtttagg
       61 cagcgcagga acgtcgactt gcccgcgccg gagaggccga tgacagcgat gaaatcgccg
      121 tcaggcacgg tgacgttcac ctcagacaac gcctgggtgc cgtccgggaa gaccttgcta
      181 aggttcgaga cctcgatcat ctggggccgt cagcctcaag tgtcaggacg ggcaaggggc
      241 cggcagcgcg caaggcggtg ccgacccctc ggttccccgt tcgcttactg gtcgagcccc
      301 aggatgcggc gggtgtcgcg caccacggcg tagtcggagc tgtcggccgg gatcatgtaa
      361 tcgatccggt agaggtccat gagcaggttg ttgtcgggcg ccttgatggt ggcgatggcg
      421 gcggcgatct ggtaggtcag ggccgcgtgc tccgacgcga actgctgcga gacgctgaac
      481 gtgtcgcccg ggatcgggcg agtgtagaag atcgcggtga tctggctggc ctggtcggcg
      541 tcgaggaact gcgtccaggc gccggagatg ttctggtcgt cgttcgcgaa ggtggccgcc
      601 gcgtcgacgg agccgttgag gacggccaga acggcggcgt cgtgcccgcc ggcgtagacc
      661 tggttgctga agaagtcgtc agggttgatg ccggctgtga tcagctcggc gcgcgggaac
      721 gtgtagccgg cggccgagtt cgggtcgatc caggcgatgg acttgccctc gaggtcgccg
      781 atgttctcga tgccgctgtc cttgcgcacg acgatggcgg accagtagta cgggttgccg
      841 gtgcgcacgg acttcaggag gacctgagcg ccgacttcgc tgttggccac aacgaagccg
      901 tcgggtggga agaaggcgaa gtcgaggttg cccgagccca tggcctcgat gaggccgcgg
      961 tagtcggtgg ggatgaagac ctgcacttcg acggccccgc ccagctggga ctcgaggtag
     1021 ttggcgatgc cctcagaggc agacgtcagc agatcggagt tctgggtcgg gttgaagccg
     1081 atcctcaccg tgatgggggc ctgcgcggcc gcgaaggaga acagcgtggc gatcgccgcg
     1141 acgaggagcg aaaacttcac gagggcttct ttcattgaac gcctcccagg gttcaacgtc
     1201 attgcgccta tgggcacttt gcgcatggag actgtagcac gcggctacgc tcggcctctc
     1261 agcaacccgg cctcgcgtgc cttggtcgcc gcctcgtacc tgctggtcac cccgagcttg
     1321 ctgatgacgt tggacacgtg gttcctgacg gtcttggccg tcacgcgcag ctccttggcc
     1381 atctcgtggt tatgcagccc gcgggcgagc agctccaaca cctcgcgctc tcgcggcgtg
     1441 aggccgaacg ccaaggggcc gccggtctgg ccgaccgaga agtagcgggc gaaccgttcc
     1501 acgatgtcgt ggtcgaacag ggcctccccg ttcgcagccg ccatgatcgc gcgctggagt
     1561 tcgtccttcg acgcgccttt gagcacgtag cccttggctc cggcccgcat ggcttggaag
     1621 accaggtcgt catcggcgaa catcgtcacc atcacgacgg ccgtaccagg gaggtccgcc
     1681 accaggctgg cgatggcctc gggcccggag aggttgggca tgcgcgcgtc catcaggacg
     1741 acccgtggcg cgaggcgtct ggccagatcg accgcctgcg cgccgtcgtg cgccatcccg
     1801 acgacctcac agcccggcat ggtggagacg agcagggtga gtccctccag gaagagctcg
     1861 tggtcgtcga ccaccaggat gcg
//
LOCUS       DAOQYE010000443         1882 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_72941_length_1882_cov_2.921730, whole genome shotgun sequence.
ACCESSION   DAOQYE010000443 DAOQYE010000000
VERSION     DAOQYE010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1882
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72941_length_1882_cov_2.921730"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            275..874
                     /locus_tag="PKN52_07605"
     CDS             275..874
                     /locus_tag="PKN52_07605"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HNQ99852.1"
                     /translation="MGKVYCDIAVSADGFATGLNQREEAPFGDIDDSWLHRWMFDTYE
                     ENRAEVDAILDSGATIMGRNMFGPVRGEWDRDWRGWWGKEPPYHGPVFVLTHHAHEPI
                     EMLGGTTFYFVTDGIRSALDQARAAAGERNISIAGGASTVNQYLAAGLLDELRLHITP
                     CILGAGERVFDGVPPQRLERVSVRAASLVTHVTYRPVRQ"
     gene            complement(878..>1882)
                     /locus_tag="PKN52_07610"
     CDS             complement(878..>1882)
                     /locus_tag="PKN52_07610"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HNQ99853.1"
                     /translation="QPEVRSVPEPAVPANGVLVRVIATGLCRSDWHAWAGHEDIAFPH
                     VPGHELAGVIVETGPQVRRWRAGDRVTVPFVCGCGHCEWCESGNAQVCPDQQQPGFTH
                     WGSFAEYVALHAADTNLVAIPAEVEFATAASLGCRFATAFRALSARARVREGEWVTVV
                     GVGGVGLSAVMIANAMGARVIAVDRNAEALAVAKQVGAKHVLLADGADIPAAVFELTG
                     GGGHVAVDAVGNEQACSDSILSLRRRGRHVQVGLLPPVSGHPRVPMARAIAWELDLLG
                     SHGMAAVDYPKMLELIERGVLQPQRLIERTIGLAEAAELLPRFDRATVAGMTMIDPAR
                     "
BASE COUNT          296 a          657 c          644 g          285 t
ORIGIN      
        1 atacttggtg tcggatcgag ctttcacctc cagtggacct ggctacctgg acggcggcgt
       61 ctaggtcccc gacgtagatg aacagttcgt gatgtaagcc atggccgggc tccactctcg
      121 cgagttggcg cggagactct tcggtggtga cccccagctc ggcgccgcca gccagaccac
      181 ctccctacgg ctggactccg gcagcgaagc gaccgcgtca ctgacgttgg cacctgatca
      241 tccgcagact gcgataaggc gaaggaggat ttcgatgggc aaggtgtact gcgacatcgc
      301 cgtctcggcc gacggcttcg cgacggggct gaaccagcgc gaggaggcgc cgttcggcga
      361 catcgacgac agctggttgc accgctggat gttcgatacg tacgaggaga accgcgccga
      421 ggtggacgcg atcctcgact cgggcgcgac catcatgggg cgcaacatgt tcggccccgt
      481 ccgcggcgag tgggaccgtg actggcgcgg ctggtggggc aaggagccgc cgtaccatgg
      541 gcccgtcttc gtgcttaccc atcacgctca cgaacccatc gagatgctgg gcggcaccac
      601 gttctatttc gtcacggacg gcatccgctc cgcgctcgac caggcccgcg ctgcggccgg
      661 cgagcggaac atctccatcg cgggtggggc cagcacagtc aaccagtact tggcagccgg
      721 tctcctcgac gagctgcgcc tccacatcac gccatgcatc ctcggtgccg gcgagcgggt
      781 gttcgacggg gtgcccccgc agaggctaga gcgggtgtcg gtgcgcgcgg cgtcgctcgt
      841 cacgcatgtc acttataggc cagtcaggca gtaggcctca gcgcgccgga tcgatcatcg
      901 tcatcccggc gacggtggcc cggtcgaacc gcggcagcag ctccgcggcc tctgcgaggc
      961 cgatggtgcg ttcgatcagc cgctgcggct gcagcacgcc gcgttctatg agttccagca
     1021 tcttcgggta gtcgaccgcg gccatgccgt ggctgccgag caggtcgagc tcccacgcga
     1081 tggcccgggc catcggcacc cgcgggtggc cggagactgg tggcaggagg ccgacctgga
     1141 cgtgccgacc ccgccggcgc aggctcagga tcgagtcgga gcaggcctgt tcgttgccca
     1201 cggcgtccac tgccacatgg ccaccgccgc ccgtcagctc gaacacagcc gcggggatgt
     1261 cggcgccgtc ggcgaggagc acgtgcttgg cgcccacctg cttggctacc gccagtgcct
     1321 cggcgttgcg gtcgaccgct atcaccctgg cgcccatggc gttggcgatc atcacggcgc
     1381 tgaggcccac cccgccgaca ccgacgacgg tcacccactc accttcacgc acgcgggcac
     1441 gcgcggacaa ggcgcggaag gcggtagcga accgacaccc gaggctggcc gccgtagcga
     1501 actcgacctc cgcagggatg gccaccaggt tcgtgtcggc ggcgtgcagg gcaacgtact
     1561 cggcgaacga gccccagtgg gtgaaccccg gctgctgctg atcggggcag acctgcgcgt
     1621 tgccggactc gcaccattcg cagtggccgc agccgcatac gaaaggcacc gtcacccggt
     1681 cgccggcccg ccaacggcgt acctgcgggc ccgtctcgac gatcaccccg gctagctcgt
     1741 ggccgggaac gtgcgggaac gcgatgtcct catgcccggc ccaggcgtgc cagtcgctgc
     1801 ggcatagccc ggtggcgatg acccgcacga gcacgccgtt ggccggcacg gcgggctcgg
     1861 gcaccgagcg gacctccggt tg
//
LOCUS       DAOQYE010000444         1882 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_72962_length_1882_cov_2.273673, whole genome shotgun sequence.
ACCESSION   DAOQYE010000444 DAOQYE010000000
VERSION     DAOQYE010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1882
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72962_length_1882_cov_2.273673"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..796
                     /locus_tag="PKN52_07615"
     CDS             <1..796
                     /locus_tag="PKN52_07615"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNQ99854.1"
                     /translation="GFLVAMALLLTLNVAAAVRERGGEVASATLGMVTGNLRHLAEAL
                     PGAGQVRVYRAGPVRWLANRLRGPVRVGLQVGHLDAGSQPDELANLRYSTGGHYAGLD
                     EVTVNAAIVDALAVRLRDRGAVVDVLPATIPPRYQADLLIAVHVDSNPDTTRSGYKSA
                     HFWPERNPRELQLKLQVDRAVFAITRMRDDDANVSGNMFEYYAFNDRRFEHAVDRGTP
                     ALLVELGYLSNPGDRGLLETPDVLAAALEEGIARYLSSVGRVPDKW"
     gene            810..1262
                     /locus_tag="PKN52_07620"
     CDS             810..1262
                     /locus_tag="PKN52_07620"
                     /inference="COORDINATES: protein motif:HMM:NF024515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HNQ99855.1"
                     /translation="MVALHQVPPERPPRRPARGQYRPGVVLLVLCALLAVGGCKRPGV
                     AGAAASGSLKVTTRLAGEAVVGVVPVVIEVSDAGVPVLGARVDVEADMTHAGMAPATA
                     TASEVGDGTYRADDLVLGMAGDWIVSVKVTTAGGVRADGELLTTVRAR"
     gene            1259..>1882
                     /locus_tag="PKN52_07625"
     CDS             1259..>1882
                     /locus_tag="PKN52_07625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99856.1"
                     /translation="MTPARHPGAVELPVAAGSYVDQPRGFDLFRLPGLRRFVRWKYAR
                     LTLQLPLLLLALFVIVDGLTGRQLAPRNVATTATWLHYRGLVVLALAIFGNAFCAACP
                     LMLTRGASRALKRFVGADVRFPRALRNKWFVLGLTLAYFIAYEAFDLWASPWLTAWLA
                     IGYFGAALVVDTLFPAGTFCRYVCPLGNFNFALSTVSPTQVVAVDPDV"
BASE COUNT          255 a          709 c          621 g          297 t
ORIGIN      
        1 gggcttcctc gtggccatgg ccctgctcct cacgctcaac gtggccgccg ccgtgcggga
       61 acgcggcggc gaggtcgcga gcgcgacgtt gggcatggtg accggcaacc tcaggcacct
      121 ggccgaagcg ctccccgggg ccggtcaggt gcgcgtctac cgcgccggac cggtgcggtg
      181 gctagccaac cgcctccgcg gccctgtgcg cgtcgggttg caggtcgggc accttgacgc
      241 cggctcccaa ccggacgagc tggcgaacct gcgttacagc acgggcggac attacgccgg
      301 cctcgacgag gtgaccgtga acgccgccat cgtcgatgcc ctcgccgtca ggctccgcga
      361 ccgtggcgcc gtcgtggacg tactgcccgc gaccatcccg ccccgctacc aggcggacct
      421 cctcatcgcg gtacacgtcg actccaaccc agacaccacg cgctccgggt acaagagcgc
      481 ccacttctgg ccggagcgca accctcgcga gctgcaactc aagctccagg tggaccgggc
      541 ggtcttcgcc atcacgcgca tgcgcgacga cgacgcgaac gtctcgggca acatgttcga
      601 gtactacgcc ttcaacgacc ggcggttcga gcatgcggtc gaccgcggca cgccggcgct
      661 gctcgtcgag ctcggctatc tctccaaccc cggcgaccgg gggctgctcg aaacgccgga
      721 cgtgctggcc gccgccctcg aggagggtat agctaggtat ctgagcagcg tgggacgcgt
      781 ccccgacaag tggtagcctg ccagcgcaga tggtcgccct gcaccaagtt ccacccgaac
      841 gcccaccacg cagaccggct cgcggacagt accggcccgg tgtcgtcctg ctcgtactct
      901 gcgcgctgct cgcggtaggc ggctgcaagc gtcccggcgt cgccggcgcg gccgcatctg
      961 gctccctgaa agtgacgacg cgactggccg gcgaggcagt cgtgggagtc gtgccggtcg
     1021 tcatcgaggt gtccgacgcc ggcgtccccg tgctgggcgc gcgggtcgac gtggaggccg
     1081 acatgaccca cgccggcatg gcgcccgcca cggcgacggc gagcgaggtc ggggacggca
     1141 cgtatcgggc ggacgacctc gttctaggca tggcgggcga ttggatcgtg agcgtcaagg
     1201 tgaccaccgc aggcggtgta cgcgccgacg gcgagctcct gactaccgtc cgagcgcgat
     1261 gacgcccgcg cgtcaccccg gtgccgtcga gctgcccgtc gccgccgggt cgtacgtcga
     1321 ccagccgcgc ggcttcgacc tcttccgact acccgggttg cgacgcttcg tccggtggaa
     1381 gtacgcccgg ctcaccctcc aactcccgct cctgctcctc gccctcttcg tgatcgtcga
     1441 cggcctgacc ggccgccagc tcgcgccgcg caacgtggct accaccgcca catggctgca
     1501 ctaccgcggc ctcgtcgtgc tcgccctggc gatcttcggc aacgccttct gcgccgcctg
     1561 cccgctcatg ctgacgcgag gggcgtcgcg tgcgctcaag cgtttcgtcg gggcggacgt
     1621 gcgcttcccc cgcgccctac gcaacaagtg gttcgtgcta ggcctcacgc tcgcgtactt
     1681 catcgcctac gaggcgttcg acctgtgggc cagcccgtgg ctcacggcgt ggttggccat
     1741 cggctacttc ggcgcggcgc tggtcgtcga cacgctcttc ccggccggca cgttctgccg
     1801 ttacgtctgc ccgctcggga acttcaactt cgccctcagc acggtctcgc ccacccaggt
     1861 cgtggccgtc gaccccgacg tc
//
LOCUS       DAOQYE010000445         1881 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_73016_length_1881_cov_2.697152, whole genome shotgun sequence.
ACCESSION   DAOQYE010000445 DAOQYE010000000
VERSION     DAOQYE010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1881
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73016_length_1881_cov_2.697152"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1139)
                     /locus_tag="PKN52_07630"
     CDS             complement(<1..1139)
                     /locus_tag="PKN52_07630"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HNQ99857.1"
                     /translation="MKTVQIGVKGMTCASCVARVERALDRVEGVQDATVNLATERATV
                     SFDPAITAPTRLLESVEKAGYEPIVGEITFGVTGMTCANCSSRVERQLQKADGVLSAS
                     VNLATERATVRYLPGQTDPTRLKHVVEDTGYGVLEADGAAERSDVEREARERELSALR
                     RDLLVAAAFTAPLLLFVMLPMLIPPLEHALMAVVPMQTVYYVSFVLAAVVQFGPGRRF
                     YRPGWRSLRALSPDMNSLVMLGSSAAFGYSVVATFLPGLLPEGTVHVYFEASAAIITL
                     ILVGKYLEAIAKGRTSEAIKKLVGLQSKTARVERDGIEMEIATDRVVPGDIVVVRPGE
                     RLPVDGVVVTGSSYVDESMITGESVPVHKGVGVGVVGGTINRTGS"
     gene            complement(1246..1578)
                     /locus_tag="PKN52_07635"
     CDS             complement(1246..1578)
                     /locus_tag="PKN52_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HNQ99858.1"
                     /translation="MEHAATTPVAGELGASAHDCKHLDQAVRLDAARRLKSAKGHLEG
                     VLRMLEDPSVYCVDVLKQVKAVQGALAKVNEEVLKSHIRDHVATASQRGDTEQIVGEL
                     MEALKYRV"
     gene            complement(1578..1781)
                     /locus_tag="PKN52_07640"
     CDS             complement(1578..1781)
                     /locus_tag="PKN52_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HNQ99859.1"
                     /translation="MTKLNVTGMSCNHCSSAVKSALEEVDGVKAVQVDLVGGTAQVEG
                     VADVDDLVAAVREAGYEASVAAA"
BASE COUNT          304 a          653 c          600 g          324 t
ORIGIN      
        1 aacgagcctg tcctgttgat ggtcccaccg accaccccga cgcccacacc cttgtgcacc
       61 ggcacgctct cgcccgtgat catcgactcg tcgacgtagg aggaaccagt caccacgaca
      121 ccgtcgacag gcagacgctc gccaggcctc accacgacga tgtcgccggg aaccacgcgg
      181 tcggtggcga tctccatctc gatgccatcg cgctcgacac gggcggtctt ggactgcagg
      241 ccgacgagct tcttgatggc ctcgctcgtc ctacccttgg cgatggcctc caggtacttg
      301 ccgacgagga tgagggtgat gatggcggcg gacgcctcga agtagacgtg aaccgtcccc
      361 tccggcagca acccggggag gaaggtggcc accacggagt aaccgaacgc agcgctcgac
      421 cctagcatca cgaggctgtt catgtcggga ctcaaggcgc gcaggctcct ccagccaggc
      481 cggtagaagc gcctgcccgg accgaactgc acgaccgcgg ccaggacgaa cgagacgtag
      541 tagacggtct gcatcggcac gaccgccatg agcgcatgct cgagcggcgg gatgagcatg
      601 gggagcatca cgaagagcag gagcggcgcg gtgaacgcgg cagcgacgag gaggtcgcgc
      661 cgcagcgcgc tcagttcgcg ctcgcgcgcc tcgcgctcga catccgagcg ctcggcggcg
      721 ccatccgctt cgaggacccc atagccggtg tcctccacca cgtgcttgag ccgagttggg
      781 tctgtctgac cggggaggta gcgcaccgtg gcgcgctcgg tagcgaggtt gacgctggcc
      841 gacaggacgc cgtcggcctt ctgcaactgc cgctccactc ggctactgca gttggcgcag
      901 gtcatgccgg tcacgccgaa cgtgatctcg ccgacgatgg gctcgtaacc agccttctcc
      961 acggactcga ggaggcgagt aggcgcggtg atagccgggt cgaagctcac cgtcgcgcgt
     1021 tccgtggcga ggttgacggt agcgtcttgc acgccttcca cccggtcgag cgcgcgctcg
     1081 acccgcgcca cgcagctggc gcaagtcatg cctttcacgc caatctggac cgtcttcatc
     1141 tgcactccca ttgcttttgc ccgtactcct gctcgtacct cggcgccgac cctctcagcg
     1201 ctcgggcggg acgcggagtg gtgggtcggt cgcgaagtgg ccgcgtcaca cgcggtactt
     1261 gagcgcctcc atgagttcgc cgactatctg ctccgtgtcg ccccgctgcg aggcggtagc
     1321 cacgtggtcg cggatgtgcg acttgagtac ctcctcgttg accttggcga gggcgccctg
     1381 tacggccttc acctgtttga ggacgtcgac acagtagacg gatgggtcct ccaacatgcg
     1441 caagacgccc tccaggtgcc ctttggcgct cttgagacgc ctggcggcgt cgagtcgcac
     1501 ggcctggtcg aggtgcttgc agtcgtgcgc cgacgcgccg agctcaccag cgaccggcgt
     1561 ggtcgccgcg tgctccatca ggcggccgcc accgacgcct cgtacccggc ctcgcgcacg
     1621 gcggccacga ggtcatcgac gtcggccacg ccttccacct gagcggtgcc gcccaccagg
     1681 tcgacctgca ctgccttcac gccgtctacc tcctcgagtg cgctcttcac ggcgctgctg
     1741 cagtggttgc acgacatccc ggtgacgttc agcttggtca tactcatcac tccttcgatc
     1801 ccttatggca ccccaccagg ggtggggtaa gctaaagaca gtgtaggcct tcgccgcccg
     1861 gtcaagtcaa gtggttacgc t
//
LOCUS       DAOQYE010000446         1881 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_73025_length_1881_cov_2.584337, whole genome shotgun sequence.
ACCESSION   DAOQYE010000446 DAOQYE010000000
VERSION     DAOQYE010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1881
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73025_length_1881_cov_2.584337"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(101..931)
                     /locus_tag="PKN52_07645"
     CDS             complement(101..931)
                     /locus_tag="PKN52_07645"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HNQ99860.1"
                     /translation="MRDASMLVAVVLAGGGQRDRLARAVGVESKAHVPLGGVPLGEYV
                     LAALRAADCVGRTVFVGDPGGSFAGLFDVRVPAGERLVDSLALGAGAALAQPGPPEWL
                     LVVTADVPWLTGPVVDRFVVAAEDLVGHDGKPARLVYPVVAEADAREQFPHQDRTYAA
                     LADGRFTGGNLVLVRADTVPALLPLMEQAYSNRKNPFALARLVGLGTLVRLLLGRASL
                     PRLERRVSHLLGAPARALVSHDASLAADVDRLEHLPGTIAADLPSLAAPADLAIGRDP
                     "
     gene            complement(1025..1753)
                     /locus_tag="PKN52_07650"
     CDS             complement(1025..1753)
                     /locus_tag="PKN52_07650"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HNQ99861.1"
                     /translation="MRPIPQQDWVTDTALYYVGDRPAQAEYGTIEFRSSPSRASVYVD
                     GSFVGYTPMRFGALAGRHDVRVESDGYEPYTESFTLRGGETKAISAQLQQVQRSGTIS
                     FTSSPNNADVYVDGRYVGTTPTGQLRFEPGTYTARFEREGFQSTSVQFTVRRGEDRTV
                     NASLAALSSSVIVRGNIGGALVFIDGRQVGTLPNGSGRLQVNDVAPGRHELVLVAPGY
                     ATYVTTFDVSAGRDTELSVRQSRF"
BASE COUNT          284 a          612 c          734 g          251 t
ORIGIN      
        1 cgcccgaggc cccagtggac gaggagggcg agcagcaggg cgaacgcgac ggccgacgag
       61 gcgccgagaa gagcggcgat gcggagctgg gctggtcgtc tcaagggtct ctcccgatgg
      121 ctagatccgc cggcgccgcg agggacggca ggtccgcggc gatggtgcct ggcaggtgct
      181 ccaagcggtc gacgtcggcg gccaggctgg cgtcgtggct gacgagcgcc cgcgccggcg
      241 cgccgagcag gtggctgacg cggcgctcca gtcgcggtaa ggacgcccgc ccgagcagca
      301 acctgacgag ggtgccgagg ccgacgaggc gcgcaagggc gaaggggttc ttgcggttgc
      361 tgtacgcctg ctccatgagt gggaggagcg ctggcacggt gtccgcacgc acgaggacca
      421 ggttcccgcc ggtgaagcgg ccgtcggcca gggcggcgta cgtgcggtcc tggtgcggga
      481 actgttcgcg cgcgtccgct tccgcgacga ccgggtagac gaggcgtgct ggcttgccgt
      541 cgtgaccgac gaggtcctcg gcggcgacga cgaagcgatc gacgaccggc cccgtgagcc
      601 acgggacgtc ggcggtgacg acgaggagcc actcaggcgg acccggttgg gcgagggccg
      661 cgcccgcgcc gagcgccagc gagtcgacaa gccgctcacc agccggcacc cgtacgtcga
      721 agaggccggc gaagcttccg ccaggatcgc ctacgaacac ggtgcggccc acgcagtcgg
      781 cggcccgcaa cgcggccaga acgtactccc cgagcggcac gccgccgagg ggcacgtgcg
      841 ctttcgactc aaccccgacg gcgcgcgcca ggcggtcgcg ttgcccgccg ccggctagga
      901 cgacggcgac gagcatgctc gcgtcacgca tgcgcggatt gtacccctca cgtgccggaa
      961 cgagcggcgc cccgccgcgt tatgccgcgg cggggcgcct tgtcggtctt cagggggcgg
     1021 gcgcctagaa gcgcgactga cgcacgctca actcggtgtc gcggccggcg ctgacgtcga
     1081 aagtcgtgac gtacgtggcg tagccggggg cgacgaggac gagctcgtgg cggccggggg
     1141 ccacatcgtt gacctgcagg cggcctgagc cgttcggcag ggtgccgacc tggcgaccgt
     1201 cgatgaacac gagggcgccg ccgatgttgc cgcgcacgat cacggatgag ctgagcgccg
     1261 cgaggctggc attgacggtg cggtcctcgc cgcggcggac cgtgaactgc acgctcgtgg
     1321 actggaagcc ttcgcgctcg aagcgggcgg tgtaggtgcc gggctcgaag cggagctggc
     1381 cggtcggagt ggtgcccacg tagcggccgt cgacgtagac gtcggcgttg ttgggcgagc
     1441 tcgtgaagga gatcgtgccg gaacgctgga cctgctggag ctgggcgctg atcgccttcg
     1501 tctcgccgcc gcgcaaggtg aaggactcgg tgtacggctc gtagccatcg gactccacgc
     1561 gcacgtcgtg gcggccggcc aacgcgccga agcgcatcgg ggtgtagccg acgaaggagc
     1621 cgtctacgta cacggaggcc ctgctcgggc tggagcggaa ctcgatggtg ccgtactcgg
     1681 cctgcgccgg gcggtctccg acgtagtaca gggccgtatc ggtgacccag tcctgctgcg
     1741 ggatgggcct cacgacgatg gcgaagctct gggcgaagcc ctgctcgccg atggtgctct
     1801 gggcgaagat gtcgctctcg cgctggaagc ggacgagcgt cgacgtgtcc agcagcgcct
     1861 agctcgacac ggcgatgacc t
//
LOCUS       DAOQYE010000447         1877 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_73296_length_1877_cov_2.655324, whole genome shotgun sequence.
ACCESSION   DAOQYE010000447 DAOQYE010000000
VERSION     DAOQYE010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73296_length_1877_cov_2.655324"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="PKN52_07655"
     CDS             complement(<1..476)
                     /locus_tag="PKN52_07655"
                     /inference="COORDINATES: protein motif:HMM:NF013768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HNQ99862.1"
                     /translation="MKERRTRVLELVAESYIATAHPVASGHIAARLEVSPATVRYDFG
                     SLESAGLLQQPHTSAGRIPTALGFRTYAASLLPPRPLAAAQQNRLDERLKGAEGDDLF
                     ALASRLAVELSGYAVTISLPADDTLHILEIHLSQLAANRLLAVVVLENGLVRQLGVT"
     gene            complement(622..1083)
                     /locus_tag="PKN52_07660"
     CDS             complement(622..1083)
                     /locus_tag="PKN52_07660"
                     /inference="COORDINATES: protein motif:HMM:NF013995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HNQ99863.1"
                     /translation="MARCWLVKSEPDCYSFDQLVADGRTAWEGVRNYQARNYMRDDMR
                     VGDLVLYYHSSTAVPAVVGIAKVASQPYPDATQFDPDNEYYDPKATEAAPRWFLVDLE
                     PVRSLPRPVTLEELRADPDLAGMQLLARGNRLSVMPVEAEHAARIQELAAA"
     gene            complement(1185..>1877)
                     /gene="guaA"
                     /locus_tag="PKN52_07665"
     CDS             complement(1185..>1877)
                     /gene="guaA"
                     /locus_tag="PKN52_07665"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HNQ99864.1"
                     /translation="ALTGVTHPEEKRKIIGREFVEVFSQEARRQQDAGPIRYLAQGTL
                     YPDVIESAGVPGAANIKSHHNVGGLPKDLGFELIEPFRALFKDEVREVAERLGLPSHL
                     RDRHPFPGPGLAVRILGDVTREKLEVLRRVDDIFISALREFDLYHDTWQAFAVLTPLR
                     SVGVMGDSRTYANTVALRAVSSVDGMTADWTRLPSEFLATVANRIVNGVPEVNRVVYD
                     ITSKPPATIEWE"
BASE COUNT          274 a          613 c          690 g          300 t
ORIGIN      
        1 gtgacaccga gctgccgcac caggccgttc tccaggacga ccaccgccag gagccggttg
       61 gctgcaagct gcgacagatg gatctcgagg atgtgcaggg tgtcgtcggc cggcaggctg
      121 atggtgacgg cgtagccgga cagctccacc gccagacgcg aggcgagcgc gaacaggtcg
      181 tcaccctccg cgcccttgag gcgctcgtcg agcctgttct gctgcgcggc ggccaggggg
      241 cggggcggga gcaagctggc ggcgtacgtg cgaaacccca aagccgtcgg tatgcggccg
      301 gccgacgtgt ggggctgctg aagcagcccg gcgctctcga gcgagccgaa gtcgtagcgg
      361 acggtggcgg ggctgacttc gaggcgcgcg gctatgtggc cggatgcgac cgggtgggcg
      421 gtggcgatgt acgactcggc caccaactcg aggacccgcg tcctacgctc cttcatgctt
      481 gacgatgcta ccaccgcgcg cgaacggctt ggtgtgtcaa gcttcgcgcg cttaccgccg
      541 ttacgaccac ccttgccgtt cgcttatggg cgtcgccgct cgccgcgctc gcgcgccgcc
      601 ggacggtgct cgtaacggcc gtcaggccgc ggccagctcc tggatgcgcg cggcgtgctc
      661 cgcctcgacg ggcatgacgc tgagccggtt gcctcgggcg aggagttgca tgcccgccag
      721 gtcgggatcg gcgcgcagtt cttccagcgt caccgggcgc ggcagggacc tgacgggctc
      781 gaggtcgacg aggaaccacc gcggggcggc ctcggtcgcc ttcgggtcgt agtactcgtt
      841 gtcagggtcg aactgcgtgg cgtccgggta cggctgagag gcgaccttgg cgatcccgac
      901 gacggccggt acggccgtgc tcgagtggta gtagagaacg aggtcgccga cgcgcatgtc
      961 gtcgcgcatg tagttgcggg cctggtagtt ccgaacgcct tcccaggccg tgcgaccgtc
     1021 ggcgacgagt tggtcgaacg agtagcagtc aggttccgac ttgaccagcc agcagcgtgc
     1081 catgccccga ctttaccctc cgcccccgga cgagtgaaga ccgggtcgac acgcgctcgc
     1141 cggacctggg agacgcgcgg cggctcacgt cagcgccggc gcgctcactc ccactcgatg
     1201 gtggccggcg gtttgctcgt gatgtcgtag acgacccggt tgacctccgg cacgccgttg
     1261 acgatccgat tggcgacggt cgccaggaac tcgctcggca gcctcgtcca gtccgccgtc
     1321 atgccgtcga cgctcgatac ggcgcgcagg gcgacggtgt tggcgtaggt gcgcgaatcg
     1381 cccatgacgc caacggagcg gagcggcgtc aggacggcga aggcctgcca ggtgtcgtgg
     1441 tagaggtcga actcgcgcag cgcggagatg aagatgtcgt cgacgcgtcg cagtacctcg
     1501 agcttctcgc gcgtcacgtc cccgaggatg cgaacggcaa ggccggggcc ggggaaaggg
     1561 tggcgatcgc gcaggtggga tggcaggccg agcctctccg ccacctcgcg cacctcgtct
     1621 ttgaagagcg ccctgaacgg ctcgatcagc tcgaagccga ggtccttcgg tagccccccg
     1681 acgttgtggt gcgacttgat gttggcggcg ccggggacgc cggccgactc gatgacgtcg
     1741 gggtagaggg tgccctgggc gaggtagcgg atgggcccgg cgtcctgctg gcgccgcgcc
     1801 tcctgcgaga acacctcgac gaactcccgt ccgatgatct tgcgcttctc ctccgggtgg
     1861 gtgacgccgg tcagcgc
//
LOCUS       DAOQYE010000448         1872 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_73603_length_1872_cov_3.035773, whole genome shotgun sequence.
ACCESSION   DAOQYE010000448 DAOQYE010000000
VERSION     DAOQYE010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1872
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73603_length_1872_cov_3.035773"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /locus_tag="PKN52_07670"
     CDS             complement(<1..551)
                     /locus_tag="PKN52_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNQ99865.1"
                     /translation="MTEASQYPSLVWHLKNTELFEDLTPDEIEQMAKITPYKRFAAGE
                     IIYHMEDPADALYFIRDGMVKISMYFPNGKEMILGLLGKYDIFGELLLLQSERRPNQA
                     EAVMDTTLIVMPEMEFQRLLQQQPRIAMKFIQVMSTRLWQAQQWQAEVGAFDAPGRLA
                     NLLLRLAKDFGTPSERGTVIDLTL"
     gene            complement(667..1731)
                     /locus_tag="PKN52_07675"
     CDS             complement(667..1731)
                     /locus_tag="PKN52_07675"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNQ99866.1"
                     /translation="MSAPTAIRVLALLLVSSFAPLRGVALAQIGEPPTTILAALEDYQ
                     LTPAEDGAGFVTASGFEFGVLASEGVAVEVNGAGLLTDANVRFLGAMVGAATGYGEGI
                     AAPVADFFRTRASELVGVGEVAVEVMEYLMYVDVSAGVIPEATVRFVAQYVDADRFGP
                     PAHVLGPADAQHVVRLFTDLQCPYCAQFEAVGMPIVQNELLPRGDVRLELYHFPLKSI
                     HPNATVAAEASECMAALARERIDFVAGEAAFWTFQNALFTNQDSWASRPDPLPLFIGI
                     AEDAGLSTEGLSECVRSGRYGAVVEESYRVAAQDLRLTGTPTVFLDGLKVGDYRDLND
                     YLRLMRLSSALAGEAEPVHP"
BASE COUNT          318 a          625 c          616 g          313 t
ORIGIN      
        1 agcgtcaagt cgatgaccgt tccgcgctcc gatggcgtgc cgaagtcctt ggcgagcctc
       61 aatagcaggt tggcgagccg ccccggcgca tcgaaggcgc cgacctcggc ctgccactgc
      121 tgagcctgcc agaggcgtgt gctcatgacc tggatgaact tcatagcgat gcgcggctgc
      181 tgttgcagca gtcgctggaa ctccatctcg ggcatgacga tgagcgtcgt gtccatgacc
      241 gcttcggcct ggttgggacg ccgctcgctc tgcaggagca gcagctcgcc gaagatgtcg
      301 tacttgccga gcagcccgag gatcatctcc ttgccgttcg ggaagtacat ggagatcttc
      361 accatgccgt cgcggatgaa gtagagggcg tcggccggat cctccatgtg gtagatgatc
      421 tcgcccgcgg cgaaacgctt gtaaggcgtt atcttcgcca tctgctcgat ctcgtccggc
      481 gtcaggtcct cgaacagctc cgtgttcttg aggtgccaaa ccagactcgg gtactggctc
      541 gcttccgtca tgctcttccc ctccgaccgt gggcgcgtcg cctggcaccg acttcctgcg
      601 ccccgacacg ctcctcatgc atcgtcgttc cgtctctcac tatcgcatac gagaagcgcg
      661 cgtcgctcac ggatgcacgg gctcggcctc gccagccagg gcgctgctga gccgcatgag
      721 ccgaaggtag tcgttcaggt cgcggtagtc accgaccttg agaccgtcga ggaagaccgt
      781 cggtgtgccg gtcaagcgta agtcctgcgc ggcgacgcgg taggactcct ctacgacggc
      841 accgtaacgc ccgctcctga cacactcgga gagcccttcg gtcgatagcc cggcgtcttc
      901 ggcgatgccg atgaacagcg gcaacgggtc aggtcggctc gcccagctgt cctggttcgt
      961 gaacagagcg ttctggaacg tccagaacgc ggcctcgccc gcgacgaagt cgatccgttc
     1021 cctggcgagc gcggccatgc actcgctcgc ctcggccgcg accgtcgcgt tggggtggat
     1081 gctcttcagc gggaagtgat agagctcgag ccgcacatcg ccgcgcggga gcagctcgtt
     1141 ctgcacgatg ggcatgccga ccgcctcgaa ctgggcgcag tacgggcatt gcaagtcggt
     1201 gaacaggcgc accacgtgct gggcatccgc cgggccgagg acgtgagccg gcggaccgaa
     1261 gcggtcagcg tccacgtact gggcaacgaa gcgcacggtg gcctcgggga tgacgcccgc
     1321 ggagacgtcg acgtacatga ggtactccat gacctcgaca gccacctcgc ccacgcccac
     1381 caactccgat gcgcgggtgc ggaagaagtc ggccaccggc gccgcgatgc cttcgccgta
     1441 gccggtggcg gcgccgacca tggcgccaag gaagcgcacg ttcgcgtcgg tcaggagccc
     1501 ggcaccgttc acttccacgg cgacgccctc ggacgcgagc accccgaact cgaagccgct
     1561 cgccgtgacg aagcccgccc cgtcctccgc gggcgtgagc tggtagtcct cgagcgccgc
     1621 caagatcgtg gtgggcggct cgccgatctg ggccaaggca acgccacgca gcggggcgaa
     1681 cgacgagacc aacagtagtg cgagcacgcg gatggcggtc ggtgcgctca tgcgaggtgg
     1741 tagagggcac cgtacttgcc ctgcaagtag tcgaggtagt gacgcgagtc cagcgccgcg
     1801 cccgttgccc gcgcgatcag atcgttcggc aagtagcgcg agccgtgcac gtgcacgttc
     1861 gagcgcaacc aa
//
LOCUS       DAOQYE010000449         1868 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_73891_length_1868_cov_2.828461, whole genome shotgun sequence.
ACCESSION   DAOQYE010000449 DAOQYE010000000
VERSION     DAOQYE010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1868
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73891_length_1868_cov_2.828461"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            21..518
                     /locus_tag="PKN52_07680"
     CDS             21..518
                     /locus_tag="PKN52_07680"
                     /inference="COORDINATES: protein motif:HMM:NF026048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNQ99867.1"
                     /translation="MPYETSPTKRVSRRELFRGGLREVAHGAADALAPLEALLPGPEG
                     EHRGQEPPPEAARRYRLMAAARPARDTLVPWRLPRVADGCIMCPACTRACPTDALERD
                     FSGPQAVLRLKPEACVGCEACVTACPVRVISMDDLITWGELSDGESEAFRSGPDRLGT
                     GALPR"
     gene            586..828
                     /locus_tag="PKN52_07685"
     CDS             586..828
                     /locus_tag="PKN52_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99868.1"
                     /translation="MTVESWVLEAVAAGSSKGSSLRDVQRYIDERHFEELAVDTIEAA
                     LATLLAAGKIYQDGERYHPAKRTSKEDAMKQLFGDG"
     gene            860..>1868
                     /locus_tag="PKN52_07690"
     CDS             860..>1868
                     /locus_tag="PKN52_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNQ99869.1"
                     /translation="MTTPVAIGSALALHDFYDIEALYDHEERLIQGSVREYVRAELLP
                     QVGEWWQEGVFPEDLPRKFGELGALGVTLPERFGGAGASYSAYGLVCKEIEYVDSGMR
                     SFVSVQSSLVMFPINAYAQDHVKAKWLPLLASGEAVGCFGLTEPDAGSDPGAMRTRCR
                     RVGDDWVITGVKRWITSGSRADVAIVWAKDEESGKILGFVVETDRPGFQAVDIKTKAS
                     MRASVTSELYLDEVGVPAGNQLQVTGLKGPLSCLNQARFGIAFGVLGAAQACFDEAAA
                     YVSDRPAFGEPLAAKQLVQSRLADMLSEITRGSLLAYRLGRLKEEGKDHPARVSLAKR
                     DN"
BASE COUNT          301 a          630 c          659 g          278 t
ORIGIN      
        1 cgatcatgtg gctcagatag atgccgtacg agacgtcgcc cacgaagcgc gtcagccgcc
       61 gcgagctgtt caggggcgga ctgcgggagg tggcgcatgg cgcggccgac gcgctcgcac
      121 cgttggaggc gctgctaccc ggcccggagg gtgagcaccg cggtcaggag ccgccgccgg
      181 aggccgccag acgctaccgg ctcatggcgg ccgcgcgccc cgcgcgcgac acgctcgtcc
      241 cttggcgcct cccgcgcgtg gcggacggct gcatcatgtg ccccgcgtgc acccgcgcct
      301 gcccgaccga cgcgctcgag cgcgacttca gcgggccgca ggcggtcctt cggctcaagc
      361 cggaagcgtg cgtcggctgc gaggcgtgcg tgaccgcctg cccggtgcgg gtcatcagca
      421 tggacgacct gatcacgtgg ggggagctga gcgatggcga gagcgaggcg ttcaggtcag
      481 gccccgatag gctcggcacg ggagcgttgc cgcgctgagc ggcgttttcg ctatccgggg
      541 catccgggag cagacctcgg ctcctccgga gcgtacactt gagccatgac cgtcgagtct
      601 tgggtattgg aagcggtcgc agcgggctcc tcgaagggat cgagcttgcg ggacgtgcag
      661 cgctacatcg acgagcgcca cttcgaagag ctcgccgtcg acaccataga agcagcgttg
      721 gctacgcttc tggcggcggg taagatctac caggacggcg aacgctacca cccggccaag
      781 cggacctcga aagaggacgc catgaaacaa ctgttcgggg acggttgagg aaacaacctc
      841 cccacctagg aggcggtcga tgacgacccc ggtggccata ggcagcgcct tggctctgca
      901 cgacttctac gacatcgagg cgctgtacga ccacgaggag cggctgatcc aagggagcgt
      961 acgcgagtac gtgcgcgccg agctcctccc gcaggtaggc gagtggtggc aggaaggcgt
     1021 cttccccgag gacctgcccc gcaagttcgg cgagctcggc gcgctcggcg ttacgctccc
     1081 ggagcgcttc ggcggcgcgg gcgcctcgta cagcgcctac gggctcgtct gcaaggagat
     1141 cgagtacgtc gactccggca tgcgctcgtt cgtgagcgtt cagtcgagcc tcgtcatgtt
     1201 ccctatcaac gcctacgccc aggaccacgt gaaggcgaaa tggttgccgc tgctcgccag
     1261 cggcgaggcc gtcgggtgct tcgggttgac cgagccggac gccggttccg atcccggcgc
     1321 catgcgcacg cgctgccgca gggtgggtga cgactgggtc atcacaggcg tgaagcgctg
     1381 gatcacgagc ggctcgcgcg ccgacgtggc catcgtctgg gccaaggacg aggagtccgg
     1441 gaagatcctg ggcttcgtgg tcgagaccga ccgcccaggc ttccaggccg tcgacatcaa
     1501 gacgaaggcc tccatgcgcg cgtcggtcac gagcgagctc taccttgacg aggtcggtgt
     1561 ccccgccggc aaccagctcc aggtcacggg cctgaaaggc ccactgtcgt gcctgaacca
     1621 ggcgcgcttc ggcatcgcgt tcggcgtgct cggcgccgcc caggcatgct tcgacgaggc
     1681 cgcggcgtac gtctcggacc gccccgcctt cggcgagccg ctggcggcga agcaactcgt
     1741 ccagtcgcgc ctggcggaca tgctctccga gatcacgcgc gggagcctcc tcgcctaccg
     1801 cttggggcgg ctcaaggaag agggcaagga ccaccctgcg cgcgtctcgc tcgccaagcg
     1861 cgacaact
//
LOCUS       DAOQYE010000450         1866 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_74019_length_1866_cov_2.947543, whole genome shotgun sequence.
ACCESSION   DAOQYE010000450 DAOQYE010000000
VERSION     DAOQYE010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1866
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74019_length_1866_cov_2.947543"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="PKN52_07695"
     CDS             complement(<1..294)
                     /locus_tag="PKN52_07695"
                     /inference="COORDINATES: protein motif:HMM:NF015944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HNQ99870.1"
                     /translation="MGGLIVLIVVAALVFYAVSIYNRIVAYEKRYQNAWSQIDVQLKR
                     RADLIPNLVETVKGYAAHESTVFEEVTRARAALMNAQGVNQSAEAANVMSAALG"
     gene            complement(401..>1441)
                     /gene="mnmA"
                     /locus_tag="PKN52_07700"
     CDS             complement(401..>1441)
                     /gene="mnmA"
                     /locus_tag="PKN52_07700"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HNQ99871.1"
                     /translation="DEGPHTLLRHRAEQGFEVVGSMLRFWPDDKPAGAFDLCCSPDAA
                     YDARRIADGLDVPFYLLDFRDTFHEIVIDPFVPTYEAGSTPNPCVWCNRHIKFGAFVA
                     RAQALGCEYMATGHFVRRVDGEHGPELHRGLDDDKDQTYFLWALPRGILKYLLFPLGE
                     LTKAQVRAMAAERSMRTAQKKSSSGLCFVPTTVKAYLDEATRAKPGRILDASNGYAVV
                     GEHRGVVYYTIGQKKGLGLHQSHLERYVVDLRAESNEVVVGTREMCHWRVLEADRRNF
                     LTSDGHVPSRVEAQVRYRQKPEPATLTLLDDDAFRLEFDDPQFAVAKGQSAVIYSGDR
                     LLGGGVIRARAG"
     assembly_gap    1442..1451
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1686..>1866
                     /locus_tag="PKN52_07705"
     CDS             1686..>1866
                     /locus_tag="PKN52_07705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99872.1"
                     /translation="MKSVRIIRLILLVALAAYLWLFHTANRQQVELPIFQSFLPPLPV
                     AYVVGFALVLGWLVGF"
BASE COUNT          295 a          607 c          635 g          319 t
ORIGIN      
        1 gccgagggcg gccgacataa cgttggctgc ctccgccgac tggttgacgc cctgcgcgtt
       61 catgagggca gcgcgcgccc tcgtgacctc ctcgaacacc gtgctctcgt gcgcggcgta
      121 gcccttcacc gtctccacca ggttggggat gaggtcggcg cggcgcttga gttggacgtc
      181 tatctggctc caagcgttct ggtaacgctt ctcgtaagcg actatccggt tgtagatcga
      241 gaccgcgtag aagacgagcg cggcgacaac gatcaggacg atgaggcctc ccatggttga
      301 acctcctgac aacgattgta gcggaaagac ccgaccatga cgtgtcgaac gccccacgcg
      361 cacgttccgg gcacgagctg gagacgaggg cggcgggttc tcagccggcg cgcgccctga
      421 tgacaccccc gccgagcagc ctgtctcccg agtagatgac cgcgctctga cccttggcga
      481 cggcgaactg cgggtcgtcg aactcgagcc ggaaggcgtc gtcgtccagg agcgtgaggg
      541 tggccggctc cggcttctgg cggtaccgga cctgcgcctc cactcggctc ggcacatgcc
      601 cgtcgctcgt caggaagttc cggcggtcgg cctccagcac gcgccagtgg cacatctcgc
      661 gcgtgccgac cacgacctcg ttgctctcgg cgcgcaggtc gacgacgtag cgctccaggt
      721 gcgactggtg gagaccgaga cccttcttct gaccgatcgt gtagtacacg acgccacggt
      781 gctcgcccac cacggcgtag ccgttggagg cgtcgaggat gcgaccgggc ttggccctgg
      841 tggcctcgtc aaggtaggcc ttcacggtgg tgggcacgaa gcagaggccg gacgagctct
      901 tcttctgcgc ggtgcgcatg ctgcgctccg cggccatggc gcggacctga gccttcgtca
      961 actcgccgag cgggaagagg aggtacttga ggatgccgcg cggaagcgcc cagaggaagt
     1021 aggtctggtc cttgtcgtca tcgagcccgc ggtgcaactc ggggccgtgc tcaccgtcga
     1081 cacgcctcac gaagtggcca gtcgccatgt actcgcagcc cagcgcctga gccctggcga
     1141 cgaacgcccc gaacttgatg tgcctgttgc accaaacgca cgggttcggc gtgctgccgg
     1201 cctcgtaggt cggcacgaac gggtcgatga ctatctcatg gaaggtgtcg cggaaatcga
     1261 gcagatagaa cggtacgtcc aagccgtcgg cgatgcgacg cgcgtcgtaa gcagcgtcgg
     1321 ggctgcagca gaggtcgaag gcgcccgccg gcttgtcgtc gggccagaaa cgcagcatgc
     1381 tgcccacgac ctcgaagcct tgctcggcgc ggtgcctcag gagcgtgtgc gggccctcat
     1441 cnnnnnnnnn ngctcggcga ggatggcggc cgccacggac gagtcgacgc caccggacat
     1501 ggccgccatg accttgaccg gtctgacggc ggccggttcg ggcgctgacg gggcgacggc
     1561 ttccacgagg ggagattgta ctttgttggg gtggacgtcg cccgggtcgg ggtgaccaaa
     1621 gcgtccgcga cggcggaacg gtccgccctc gcgcgcaacc tgtagactac ggcccggagg
     1681 tggccgtgaa gagcgtccgc atcatcagac tcatcctcct ggtcgcgttg gctgcgtacc
     1741 tgtggctctt ccacaccgcg aacaggcaac aggtcgagtt gcccatcttc cagagcttcc
     1801 tgccgccgct gcccgtggcg tacgtggtcg gattcgccct cgtgctcggt tggctcgtcg
     1861 ggttcc
//
LOCUS       DAOQYE010000451         1862 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_74311_length_1862_cov_2.434975, whole genome shotgun sequence.
ACCESSION   DAOQYE010000451 DAOQYE010000000
VERSION     DAOQYE010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1862
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74311_length_1862_cov_2.434975"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="PKN52_07710"
     CDS             complement(<1..358)
                     /locus_tag="PKN52_07710"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99873.1"
                     /translation="MTALHLAKAALLAVPLWLLLSSVMRFKPSWLRHLASLAVGFGAA
                     ALITLVMPTGLLSYVTTFILMACVYAVLSVGLNVQWGYTGLFNIGIAAFYAIGAFTSA
                     LVTTKMPTGLNASFTKQ"
     gene            complement(360..1370)
                     /locus_tag="PKN52_07715"
     CDS             complement(360..1370)
                     /locus_tag="PKN52_07715"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNQ99874.1"
                     /translation="MERFRRAAAGRTGMLVALALVLAAIVVVAIVKGVPGTQLLSLFV
                     RGVLLGGTLALGAIGVTLVYAVLKMANFAHGDTMTLGAYLGLVVVTLLPKGAPLAPFS
                     FGYEFLVALLLVIPAVGLIAFGLDQLAFRPLRARRSQSIMFAMAALGLAFGIRSLVYI
                     FWGADFTFFYTGRARPAVELLAGVRVRPDQLFILGLAVVLIVGVYLLLERTKIGKAMR
                     ATADNSDLARISGIDTKRVILWTWLIGGGLAGAGGMLYGLDVQLRPEMGWWLLLPLFA
                     AVILGSIGNAYGALAGAFVIGIVWQMSGAFINPVYSQGIAFLVMIIVLIVRPEGLFGG
                     RK"
     gene            complement(1409..>1862)
                     /locus_tag="PKN52_07720"
     CDS             complement(1409..>1862)
                     /locus_tag="PKN52_07720"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF016362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HNQ99875.1"
                     /translation="GDPANGDGGVIAYRDPLGLEPPIPKVQRKRTLNTIGGPDVQKLM
                     GTLAPGSTEVGLFITLGTFTSDTISLARSRHDLRSVNGRQLVDMIFDKIVEINRTGVA
                     VLMVEQNAKKSLALANRGYVLATGQNQVDGQGRDLLENPEVARLYLGG"
BASE COUNT          331 a          597 c          668 g          266 t
ORIGIN      
        1 gctgtttcgt gaagctggcg ttgaggccgg tcggcatctt ggtcgtgacg agcgcgctcg
       61 tgaacgcgcc gatggcgtag aacgccgcga tgccgatgtt gaagaggccc gtgtatcccc
      121 actgcacgtt gaggccgacc gacagcacgg cgtagacgca cgccatcagg atgaaggtcg
      181 tgacgtaaga gaggaggccg gtcggcatga ccagcgtgat gagcgccgcg gcgccgaagc
      241 cgacggcgag cgacgcgagg tgcctgagcc aggacggctt gaagcgcatg acgctggaga
      301 ggaggagcca caggggcacc gccaggagcg cggccttggc gaggtgaagc gccgtcatgc
      361 tacttcctcc ccccgaacag tccctctggg cggacgatga gcacgatgat catgacgagg
      421 aaggcgatcc cctggctgta gacggggttg atgaacgcgc cgctcatctg ccacacgatg
      481 ccgatcacga acgctccggc gagggccccg taggcgttgc cgatgctgcc gaggatgacg
      541 gccgcgaaga gcggcaggag cagccaccac cccatctcgg gcctgagttg cacgtcgagg
      601 ccgtagagca tgccgcctgc accggccagt ccgccgccga tgagccacgt ccagaggatg
      661 acgcgcttcg tgtctatgcc gctgatgcgc gccaggtccg agttgtcggc ggtggcgcgc
      721 atggccttgc cgatcttggt gcgctcgagc agcaggtaca cgccgacgat gagcacgacg
      781 gccaggccca ggatgaacag ctggtccggt cgcacccgca cgccggcgag gagctcgact
      841 gccggccgcg cgcgccccgt gtagaagaac gtgaaatccg cgccccagaa gatgtagaca
      901 aggctcctga tgccgaacgc gaggccgagg gcggccatcg cgaacatgat cgactgcgag
      961 cgacgggccc gcagcggccg gaaggcgagc tggtcgagcc cgaaggcgat gagcccgacg
     1021 gccgggatga ctaggagcag ggcgacgagg aactcgtacc cgaaggagaa cggcgccagc
     1081 ggcgcccctt tgggcagcag cgtcacgacg acgaggccga ggtacgcgcc gagcgtcatg
     1141 gtgtcgccgt gcgcgaagtt ggccatcttg aggaccgcgt agacgagcgt gacaccgatg
     1201 gcgccgagcg ccagcgtgcc gccgagcagc acccccctca cgaagagcga gaggagctgc
     1261 gtgccgggca cgcccttgac gatcgcgacg accacgatgg cggcgagcac cagcgcgagc
     1321 gccacgagca tgcccgtccg gcccgccgcg gcgcggcgga accgttccat gggtcgcgcg
     1381 cttgaggatc ccgtactctg caccaccgtc aacctcccag gtagaggcgg gcgacctctg
     1441 ggttctcgag gaggtcgcgg ccttgaccgt cgacctggtt ctggccggtc gccaggacgt
     1501 agccccggtt ggccaacgcg agcgacttct tggcgttctg ctcgaccatg aggaccgcca
     1561 cgcccgtgcg gttgatctcg acgatcttgt cgaagatcat gtcgaccagc tgacggccgt
     1621 tcaccgagcg caggtcgtgt cgactgcggg ccagggagat cgtgtcgctc gtgaaggtgc
     1681 cgagggtgat gaacaggccg acctctgtgc tgccgggagc cagagtcccc atgagcttct
     1741 gcacgtcggg cccgccgatc gtgttgagcg tccgcttgcg ctggaccttg gggatcggtg
     1801 gctccaagcc cagcggatcc cggtaggcga tgacgccgcc gtcgccgttg gcggggtcac
     1861 ct
//
LOCUS       DAOQYE010000452         1860 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_74438_length_1860_cov_2.435457, whole genome shotgun sequence.
ACCESSION   DAOQYE010000452 DAOQYE010000000
VERSION     DAOQYE010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74438_length_1860_cov_2.435457"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..354)
                     /locus_tag="PKN52_07725"
     CDS             complement(<1..354)
                     /locus_tag="PKN52_07725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99876.1"
                     /translation="MPRYPLAGWAHIIIFFGFCVLLLNSIMLWVRGYVSTDSAAYDLW
                     IFGVDQPLGMIYAFLRDIFTVLVILGVLVYFYYRLVARLPRLTLNFEGVLILLIIFTM
                     MVADLVYEGVEINKHH"
     gene            complement(587..1285)
                     /locus_tag="PKN52_07730"
     CDS             complement(587..1285)
                     /locus_tag="PKN52_07730"
                     /inference="COORDINATES: protein motif:HMM:NF014630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HNQ99877.1"
                     /translation="MLNQLAEVQAFDLKLDSLREERGQVPPELTATEAKKRDLEARIA
                     LKEAAQDELRKRVNANELELKALQERRRSAADSAVRASTAKEASQYQNQELQFATRLT
                     ELEEDTLPLLESQSALDAEVTALHAELAALLPELDALRQAEADRVAEVDARGVSIAQE
                     RAALAHGITPALLQQYEQVRKSKRGLGLAEVVGGGTCGGCNVRLPIHVVQKVKRAGEV
                     TRCPSCGRILYNKS"
     gene            1414..>1860
                     /locus_tag="PKN52_07735"
     CDS             1414..>1860
                     /locus_tag="PKN52_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99878.1"
                     /translation="MLEDSPEGFASRWSRYAAEVELFPRTEGNPLIECRAGDVMLQLF
                     ERTGPYLSRPGARRVIIHPLTDAVERLEPGAEPTLLANGISSLQAQGTVVATEGEFLV
                     VDAGVPLVVGVRGEPLSGLRVGDSVRVDCPAPVHGFAYSTLQPLERR"
BASE COUNT          286 a          646 c          613 g          315 t
ORIGIN      
        1 gtggtgcttg ttgatctcga cgccctcata caccaggtcc gcgaccatca tcgtgaagat
       61 gatgagcagg atgagcaccc cttcgaagtt cagcgtcagg cgtggcagcc gggcgacgag
      121 gcggtagtag aagtacacga gcacgccgag gatgaccagt acggtgaaga tgtcgcgcag
      181 gaaggcgtag atcatcccga gcggttggtc cacgccgaag atccacaggt cgtacgccgc
      241 gctgtcggtc gagacgtacc cacgcaccca caacatgatc gagttcaaca gcagcacgca
      301 gaagccgaag aagatgatga tgtgtgccca gccggccagc gggtagcgcg gcatccgcac
      361 ctggccgaac acgtacttga tgacgcccgc gacccgctcg ccgtgctccc ctgagcgtca
      421 cggcgcgccc ctgactcaga agcgcgggct aagcggtctc gccgtcgcgt cgaggctcgc
      481 aagctccccg cggcgcgcca ctaggcgagg ttgagcaagc catgcgaacg gtcccgcgac
      541 cacgcggtca cgggaccttc tcgtcggctg acccgctggg gaagcctcag gacttgttgt
      601 agaggatccg gccgcagctc gggcagcgcg tcacctcacc ggcacgcttc accttctgga
      661 cgacgtggat gggcaggcgg acgttgcacc cgccgcacgt gccgccgccc acgacctcgg
      721 cgagcccgag gccgcgcttg gacttgcgga cctgctcgta ctgctggagc agtgcagggg
      781 tgatgccatg cgccaaggcc gcgcgctcct gcgcgatgct gacgccgcgc gcgtccacct
      841 cggccacccg gtcggcctcg gcctgcctca gggcgtccag ctcgggcaac agcgcggcca
      901 actcggcgtg cagcgccgtc acctcggcgt cgagcgccga ctggctctcg agcaacggta
      961 gcgtgtcctc ctcgagctcg gtgagccgcg tggcgaactg cagctcctgg ttctggtact
     1021 gcgacgcttc cttggccgtg gaagcccgca cggcggagtc cgcggcggac cggcgccgct
     1081 cctgcagcgc tttcagctct agttcgttgg cgttgacgcg cttccggagc tcgtcctggg
     1141 ccgcctcctt gagggcgatc cgggcctcca ggtcgcgttt cttggcttct gtggccgtga
     1201 gttccggagg aacctgaccg cgctcttcgc gcaagctgtc cagcttgagg tcgaacgctt
     1261 gtacttcggc cagttggttg agcatcatgc ctccggtaac gaaaaacccc cgcccgtgtg
     1321 cggctgggcg agggtccggt ctacacctag gtacttcggt cgagtcacgc cctggagtgt
     1381 agcacgcgct ctcccgggtt agacttcggc gatatgctcg aggactcgcc tgaaggcttc
     1441 gcttcgcgtt ggtcacgcta tgcggccgag gtcgagctgt tcccgcgcac cgaaggcaac
     1501 ccgctcatcg agtgcagggc aggcgacgtc atgctccaac tcttcgagcg caccggccct
     1561 tacctgtctc gccccggcgc gaggcgcgtg atcatccacc cgctgacgga cgccgtcgag
     1621 cggctcgagc ccggcgccga gccgaccctc ctcgccaacg gcataagcag cctccaggcg
     1681 caaggcacgg tcgtggccac cgaaggcgag ttcctcgtgg tggacgcggg ggtgcctttg
     1741 gtcgtcgggg tgcgcggcga gcccctctcg ggcctgcgcg tcggtgactc cgtacgcgtc
     1801 gactgccccg cgcccgtgca cggcttcgcc tactccactt tgcagccgct cgagcgccgc
//
LOCUS       DAOQYE010000453         1859 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_74512_length_1859_cov_1.952882, whole genome shotgun sequence.
ACCESSION   DAOQYE010000453 DAOQYE010000000
VERSION     DAOQYE010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74512_length_1859_cov_1.952882"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..743
                     /locus_tag="PKN52_07740"
     CDS             <1..743
                     /locus_tag="PKN52_07740"
                     /inference="COORDINATES: protein motif:HMM:NF013263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99879.1"
                     /translation="AGAELLIYPTAIGYESSDSQEEMERQFGAWQTIQRGHAVANGLP
                     VVVVNRTGYEPDWSEVTGGIRFFEFTDRLPGALEVFHGIKRVADAELPGMVIGAGTIT
                     KVERAAAFVDAGAEFLVSPVLAPQVLAWSRERGVPFVPGAASPTEVWRALEDGAGVVK
                     LFPAGPLGPAYLKSLLGPLPCKVMVTGGVSATPEAVRTWLGTGAVAVGLGSDLVPRGR
                     LTSAQLADLTRKCEGLTVAVAALRGPAA"
     gene            740..1801
                     /locus_tag="PKN52_07745"
     CDS             740..1801
                     /locus_tag="PKN52_07745"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HNQ99880.1"
                     /translation="MSKPLVVTFGEAMLRLSAPRAQPLHLTDSLSVHVGGAEANVAAG
                     VASLGLRARWYSRVARGELGERILSDLAQHGVDTACVARGAERTGLYFFEEGSGARPA
                     SVIYDRAGSAFTAVLEADAAAAVEAGLLDGAAAFVTSGITLALGDGPRAAAAYLWRRA
                     GHGGAVRVLDINYRSRLATPAEAAAQAAPLLASADIVVVAERDLGPLFGGLAGLRAAA
                     GTARIVITQGAAGASTVLPGTAAGAHGTLTGAPGTAAGAPDGTTVAQGALPSADEGRI
                     GRGDAFLAGYLTAHLEGGDVREALAFGVASASLKSTQASDLPALDRARVEALAEEALA
                     LAQPDTVRAYEGSAPDVQR"
BASE COUNT          230 a          660 c          720 g          249 t
ORIGIN      
        1 tggccggggc tgaattgctg atctacccta cggcgatcgg ttatgaaagc agcgactcgc
       61 aggaggaaat ggaacgccag ttcggcgcct ggcaaaccat acagcggggc catgccgtgg
      121 ccaacgggct gcctgtcgtc gtcgtcaacc gcaccggcta cgagcccgac tggagcgagg
      181 tgaccggcgg catccgcttc ttcgagttca ccgaccgcct gccgggcgcg ctcgaggtgt
      241 tccacggcat caagcgcgtc gccgacgcgg agctgcccgg catggtcatc ggcgcgggca
      301 ccatcactaa ggtggagcgt gcggccgcgt tcgtggacgc cggcgcggag ttcctcgtga
      361 gccccgtcct cgcgccccag gtcctcgcct ggagccggga gcggggggtg ccgttcgtgc
      421 ccggcgcggc ctcgcccacc gaggtgtggc gggcgttgga ggacggcgcc ggcgtcgtca
      481 agctcttccc cgccggcccg ctcggacctg cctacctcaa gagcctcctc gggccgctgc
      541 cgtgcaaggt catggtcacc ggtggcgtca gcgccacccc cgaggcggtc aggacctggc
      601 tcggcacggg cgccgtcgcg gtcggcctag gctccgacct ggtgccgcgc ggccgcctca
      661 cgtccgcgca gctcgccgac ttgacgcgca agtgcgaggg gctcacggtc gcggtggcgg
      721 cgcttagggg cccggcggcg tgagcaagcc cctcgtcgtc acgttcggcg aggccatgct
      781 gcggctgtcc gcgccgcgcg cgcagccgct gcacctcacc gacagcctga gcgtgcacgt
      841 cggtggcgcg gaggcgaacg tggcggcggg cgtcgcgagc ctcggcctgc gcgcacggtg
      901 gtactcgcgc gtggcgcgcg gtgagctcgg cgagcggatc ctgagcgacc tggcgcagca
      961 cggcgtcgac accgcatgcg tggcgcgcgg cgctgaacga acggggctct acttcttcga
     1021 ggagggctcg ggcgcgcggc cggcgagcgt catctacgac cgggccggct cggccttcac
     1081 ggccgtgctc gaggcggacg ccgcggcggc cgtcgaggcg ggtctgcttg acggggcggc
     1141 ggccttcgtg acgagcggca tcacgctggc cctcggcgac gggccgcgcg cggccgcggc
     1201 gtacctctgg cgccgcgcgg ggcatggtgg ggcggtgcgc gtgctggaca tcaactatcg
     1261 cagccgcctg gccactccgg cggaggctgc ggcacaggcg gcgccgctgc tcgcgtctgc
     1321 cgacatcgtc gtcgtggccg agcgcgacct gggtccgctc ttcggcggcc tggcgggcct
     1381 acgcgcggcg gctggcacgg cgcgcatcgt gatcacgcag ggcgcggcgg gcgcctcgac
     1441 ggtgctcccc ggcacggctg ccggggcgca cggcactctg acaggggcgc ccggtacggc
     1501 tgccggggcg cccgacggca cgacggtcgc gcagggcgcc cttccctcgg ccgacgaggg
     1561 gcggatcggc cgcggcgacg ccttcctggc gggttacctg acggcgcacc tcgaaggggg
     1621 cgacgtccgc gaggccctcg cgttcggcgt ggcgtcagca agtctcaagt cgacgcaagc
     1681 gtccgacctg cccgcgctag accgcgcacg tgtggaggcg ttggccgagg aggccctggc
     1741 actcgcccag ccggacacgg tacgagccta cgagggctcg gcgccggacg tgcagcgcta
     1801 ggcgcacgga ggccccgtga cgagtgcaac gggaaccggg gtactagaga gcacaacgc
//
LOCUS       DAOQYE010000454         1854 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_74809_length_1854_cov_3.633130, whole genome shotgun sequence.
ACCESSION   DAOQYE010000454 DAOQYE010000000
VERSION     DAOQYE010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1854
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74809_length_1854_cov_3.633130"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1203
                     /locus_tag="PKN52_07750"
     CDS             <1..1203
                     /locus_tag="PKN52_07750"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNQ99881.1"
                     /translation="EDYLLGYAMTLAPLAIDGKILVGASGGEYGIRGFVAAYDAETGD
                     LLWKTHTIPGPGEPGNETWPGDTWKTGGASIWVTGSYDPDLNLTYWGTSNAAPWSGAL
                     RPGDNLYTASVVALDPDTGEIRSHYQYVPNDTWDYDEISDQTLVDIVRDGQPLKGLIH
                     AARSGNFYLLDRTNLDFVYGTPYMEAEPMTITGFEADGRPIIPEEHRPDIGKSVDVCP
                     ALGGANNWFGLAYSPDTGYAYVPTSESCMRIEGGEADYMAGQTYMGANFSIYIPDDLD
                     EAGALQAIDVATGEVVWKHRQQMPVRSPAMVTAGGLVFGGDIFDRELRAYDAKTGEIV
                     WRFKTNSGVLGVPVSFELDGVQYIAVEAGHGAIAAGDVALAAATYGVPFNPVSGGAVW
                     VFALKSAR"
     gene            1317..>1854
                     /locus_tag="PKN52_07755"
     CDS             1317..>1854
                     /locus_tag="PKN52_07755"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HNQ99882.1"
                     /translation="MTWELRVCADQDNLPYSNRAGDGFENRLARLVAEELGADLTYAW
                     MPLPRQGTDAELMLRLGECDVLIGAADGQEPFLNTIDYYSNATYFVTRSDGGATIESL
                     DDPRLLGLQIGVDRGGPLDFALRSRVPQSNLHHYLASDGADAILRDVAAGRLDVGIVR
                     GAAAGYFAEAGATPMTVAP"
BASE COUNT          323 a          647 c          593 g          291 t
ORIGIN      
        1 gaggactacc tcctcggcta cgccatgacc ctcgctccgc tggccatcga cggcaagatc
       61 ctggtcggcg cgtcaggcgg ggagtacggc atccgcggct tcgtggccgc ttacgacgcc
      121 gagacgggcg acctcctctg gaagacccac accatcccgg gaccgggcga gccgggcaac
      181 gagacctggc cgggcgatac ctggaagacg ggcggcgcct ccatctgggt cacgggctca
      241 tacgaccccg acctgaacct gacctactgg ggcaccagca acgcggcgcc gtggtcgggc
      301 gctctgcggc ccggcgacaa cctctacacc gcgtcggtcg tggccctcga ccccgatacc
      361 ggcgagatcc gttcgcacta ccagtacgtt cccaacgaca cgtgggacta cgacgagatc
      421 tcggatcaga ccctggtcga catcgtgcgc gacggacagc cgctcaaggg cctcatccac
      481 gcggcgcgta gcggcaactt ctacctactg gaccgcacca acctcgattt cgtgtacggc
      541 acgccgtaca tggaggcgga gcccatgacc atcacaggct tcgaggcgga cggccgaccc
      601 atcatccctg aggagcaccg gcccgacatc ggtaaatccg tggacgtctg cccggcgctt
      661 ggcggcgcga acaactggtt cggactggcc tacagcccgg atacgggtta cgcctacgtg
      721 ccgacctcgg aatcgtgcat gaggatcgag ggcggcgagg ccgattacat ggcgggccag
      781 acctacatgg gcgccaactt cagcatctac atcccggacg acctcgacga ggccggcgcg
      841 ctccaggcca tcgacgtggc gaccggcgag gtcgtctgga agcaccgcca gcagatgccc
      901 gtacgctcgc ctgccatggt cacggcgggg ggcctggtgt tcgggggcga catcttcgac
      961 cgcgaactca gggcctacga cgccaagacc ggcgagatag tctggcgctt caagacgaac
     1021 tcgggcgtcc tcggcgtccc ggtatcgttc gagctcgacg gggtccaata catagccgtc
     1081 gaagccggcc acggcgctat cgccgcgggc gatgtcgccc tggcggcagc cacgtacggc
     1141 gtgccgttca acccggtcag cggcggcgcg gtctgggtgt tcgccctcaa gtctgcgaga
     1201 tgacgtcact tgccaggttt ctcctcaccc acgatcgggt gaggagaaac ctgtgcgtcg
     1261 cgatcgcggc gatcggctta ctagcaaccg ccgcggtgga ggctcaaacc ggtccagtga
     1321 cctgggagct gcgcgtctgc gccgatcagg acaacctccc ttactcgaac cgggcaggcg
     1381 acggcttcga gaaccgcctg gcacggttgg tcgccgagga gctcggcgcc gacctgacct
     1441 acgcttggat gccactgccg aggcagggta ccgacgcgga actcatgctg cgtttgggtg
     1501 aatgcgacgt gctcatcggt gccgccgacg gacaagagcc gttcctcaac accatcgact
     1561 actactccaa cgccacgtac ttcgtcacgc gctcggatgg tggcgctacc atcgagtccc
     1621 tcgacgaccc acgccttcta ggcttgcaga tcggcgtgga ccgtggcggt ccactagact
     1681 tcgcgctcag gtctcgggta ccccagtcga acctccacca ttacctcgcg agcgacggcg
     1741 ccgacgccat cttgcgcgac gtggcggccg gtcgcctcga cgtcggcatc gtgcgcggcg
     1801 ccgccgcggg ttacttcgcc gaagccggag cgacccccat gacggtcgca ccgg
//
LOCUS       DAOQYE010000455         1848 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_75223_length_1848_cov_2.513107, whole genome shotgun sequence.
ACCESSION   DAOQYE010000455 DAOQYE010000000
VERSION     DAOQYE010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1848
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75223_length_1848_cov_2.513107"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="PKN52_07760"
     CDS             complement(<1..283)
                     /locus_tag="PKN52_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99883.1"
                     /translation="MTGSRRFAAGTIALVLIGYVSIVVAWLHLGLPSPDHSIKDHHLQ
                     HLFFLIGGGLWGIALARWLARRHPHGQQSAWLAPALLAPMAAMFLMWPSA"
     gene            complement(280..1797)
                     /locus_tag="PKN52_07765"
     CDS             complement(280..1797)
                     /locus_tag="PKN52_07765"
                     /inference="COORDINATES: protein motif:HMM:NF012343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HNQ99884.1"
                     /translation="MASITGTRNPSHLLAPLALLAVVLLMVVGLLMRMSQAGWIRLTS
                     VDFYALMTLHGAGMIAALVLCGMGGLWYLIRREVAMSASLALWAYGLAVGGVVLVAVA
                     AAFGKFGAAWTFLYPLPFLNPTWPAWSIGAFLVGMTLIVLGWSVWCIQMLEAVLRGFG
                     GFRGVLGWDAVFTPKAFAASGRKLPPPQVLPAFVIALDGLLAAAAASLLGVALLVHWL
                     DPSVPLDALWAKNITYFFGHDLANFIIYMLIAQVYVGLPAYARRPWKNTRVLTLGWWG
                     TLVFLLLAFPHHLYMDFAQPIWIQYLGLGASYVSSIPVAVVTVFGGLLLVWRSGMRWG
                     LGSIFMYTGMAGWILGGIGALLDATIQFNLHLHNTLWVPAHFHTYLLGASFLFAIGWV
                     FAMLEGRSENPTSSAMRWTIGATVLGGIGLLVATFYLAGAASVPRRYDVEPAPGPVFA
                     GWGSVGAIILLIGLAVAFFEGMRIWRGLKQARTHVEWPWSPEPATQTARSGGGAR"
BASE COUNT          374 a          587 c          659 g          228 t
ORIGIN      
        1 aggcggacgg ccacatgagg aacatcgcgg ccatcggcgc cagtagcgcg ggggcgagcc
       61 atgcactctg ctgaccgtga ggatgcctac gcgccaacca ccgcgcaagc gcgatgcccc
      121 acaggccgcc gccgatgagg aagaacaggt gctgcaggtg gtggtccttg atgctgtggt
      181 ctggcgaggg taggcccagg tggagccagg ccacgacgat ggagacgtac ccgatcagga
      241 cgagggcgat ggtgcccgcc gcgaagcgcc tggagcccgt catctggcac ctccaccaga
      301 gcgggcggtt tgcgttgcgg gttctggcga ccagggccac tcgacatgcg tccgggcttg
      361 cttcagcccc cgccagatcc tcatcccctc gaagaacgcg acagccaatc cgatcagcag
      421 gatgatcgcc ccgaccgaac cccagccggc gaacaccgga cccggcgcgg gttcgacgtc
      481 gtagcggcgc ggtacgctcg cggcgcccgc caggtagaac gtggccacga ggagcccgat
      541 gccgcccagc accgtggcac ctatggtcca gcgcatcgct gaggaggtcg ggttctcgct
      601 ccgcccctcg agcatggcga agacccagcc gatggcgaag aggaacgacg cccccagcag
      661 gtacgtatgg aaatgggcag gcacccagag cgtgttgtgc aggtgcaggt tgaactggat
      721 ggtcgcgtcg agcagtgcgc cgatgccgcc caggatccag ccggccatgc ccgtgtacat
      781 gaagatcgag ccgagacccc agcgcatgcc ggagcgccag acgagcagga ggccgccgaa
      841 gacggtgact accgccaccg gtatcgacga cacgtaggag gcccctagtc ccaggtactg
      901 gatccagata ggttgcgcga agtccatgta gagatggtga gggaacgcga gcagcaggaa
      961 caccagcgtg ccccaccatc cgagcgtgag cacccgcgtg ttcttccacg ggcggcgggc
     1021 atacgcgggc agcccgacgt agacctgcgc gatgagcatg tagatgatga agttagcgag
     1081 gtcgtgaccg aagaagtagg tgatgttctt cgcccagagg gcgtcgagcg gcacggacgg
     1141 gtcgagccag tggacgagca gtgccacccc gagcaggctg gcggcggcgg ccgccaggag
     1201 tccgtcgagg gcgatgacga aggcgggcag gacttgcgga ggcggtagtt tgcgtcccga
     1261 cgccgcgaac gccttgggtg tgaagaccgc gtcccagccg aggacgcctc ggaagccccc
     1321 gaagccgcgc agcacggcct cgagcatctg gatgcaccac accgaccagc caaggacgat
     1381 cagggtcatg ccgaccagga aggcgccgat cgaccaggcc ggccaggtcg ggttcaggaa
     1441 cggcagaggg tacaggaagg tccaggctgc gccgaacttg ccgaacgcgg ccgcaaccgc
     1501 gacgagcacc acgccgccga cggcgagccc gtatgcccat agtgcgaggc tcgcggacat
     1561 ggcgacctcg cggcggatca gataccacag cccgcccatg ccgcagagca ccagggctgc
     1621 gatcatgccg gcgccgtgca gggtcatcag cgcgtagaag tcgacagaag tcaggcggat
     1681 ccagccggcc tgggacatgc gcatgaggag gccgacgacc atgagcagca cgaccgccag
     1741 cagcgcgagc ggcgcgagca ggtgcgaagg gttgcgggtt cccgtgatgg aggccatggc
     1801 ggtttagaag acgagctgac ggatgcgcac gaggtaggcg agcacctc
//
LOCUS       DAOQYE010000456         1847 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_75265_length_1847_cov_3.619420, whole genome shotgun sequence.
ACCESSION   DAOQYE010000456 DAOQYE010000000
VERSION     DAOQYE010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75265_length_1847_cov_3.619420"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..718
                     /locus_tag="PKN52_07770"
     CDS             <1..718
                     /locus_tag="PKN52_07770"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ99885.1"
                     /translation="VSDLVQGDLGVSARTSNAVTLEIGVRFPATLKLALVSTALAVLI
                     GIPLGAFAAVRAGRLPDLLVSTVVLFGISMPVYWLGLMLIILFSIRLRWLPAAGADGV
                     LAVILPAVTLAFFSMAFVARITRSSMLEVLHQDYVRTARSKGVSPPAVVWRHAMRNAL
                     APVITVIGLQFGELLGGAILTETVFAWPGLGRLLVDSIFARDYPVVQGLVIVFALLFA
                     LVNLGIDLMYGVIDPRVKYG"
     gene            726..1601
                     /locus_tag="PKN52_07775"
     CDS             726..1601
                     /locus_tag="PKN52_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ99886.1"
                     /translation="MTRFDEVRAGVTVGAGTFKRLLRSPSALVGLVIVVLLLAAALLA
                     PVLTDYDPVKQNLREVLKAPSTAHPLGTDQLGRDILARLLFGARLTLFIGAFAVGVGL
                     AVGVPLGVISGYYRGTLDFVIQRVMDLMLSFTTFLLALTLVALLGVGLTNVIVAVGIS
                     TIPRFARLVRSSVLSIREVTYIEASMALGVPHRRVLWRHVLPNALAPVIVQATLSMGA
                     TILTAAGLGFLGLGVQPPTPEWGAMLGEGRNYIFSQPSVTTFPGLAIFLAVVGFNLLG
                     DGLRDALDPQLRGRR"
BASE COUNT          232 a          672 c          578 g          365 t
ORIGIN      
        1 cgtctccgat ctggtgcagg gcgacctcgg cgtctcggcg cgcacctcca acgccgtcac
       61 cttggagatc ggcgtgcgct tccccgccac gctcaagctc gccctggtca gcaccgcgct
      121 ggccgtgctc atcggcatac cgctcggcgc cttcgcggcc gtgcgggcgg ggcgcctgcc
      181 tgatctcctc gtgtctaccg tggtgctgtt cggcatcagc atgcccgtct actggctcgg
      241 gctgatgctc ataatcctct tctccatacg cctacgctgg ctgcccgcgg ccggggcgga
      301 cggcgtgctg gccgtgatac tcccggccgt tactctggcc ttcttctcga tggcgttcgt
      361 ggcccgcata acaaggtcga gcatgctcga ggtcctccac caggactacg tgcgcacggc
      421 ccgctccaag ggcgtgtctc ctccggccgt cgtgtggcgc cacgccatgc ggaacgcgtt
      481 ggcgcccgtc atcaccgtca tcggcttgca gttcggcgag ttgctggggg gcgccatcct
      541 gaccgagacg gtgttcgcgt ggccggggct cgggcggctc ctcgtcgatt ccatcttcgc
      601 gcgcgactac ccggtcgtgc agggcctcgt catcgtcttc gcgctcctct tcgcacttgt
      661 aaacctgggc atcgacctca tgtacggcgt catcgacccg cgggtcaagt atggctaggg
      721 cgtcggtgac acggttcgat gaggtcaggg ccggcgtgac cgtcggcgcc ggcacgttca
      781 agcggttgct gcggagcccc agcgcgctgg tgggcctcgt catcgtggtc ctcctgctcg
      841 cggcggcgct cctcgctccg gtcctcacgg actacgaccc cgtcaaacag aacctgcggg
      901 aagtcctgaa ggcgccgtca acggcgcacc ctctcggcac ggaccagttg ggccgcgaca
      961 tcctcgcccg gctgctgttc ggtgcgcgcc tgacgctctt catcggggcg ttcgccgtgg
     1021 gcgtcggttt ggccgtgggg gtgcccctcg gagtgatctc cggctactac cgcggcacgc
     1081 tcgacttcgt catccagcgc gtgatggacc tcatgctctc gttcaccacc ttcctgctgg
     1141 ccctcaccct cgtcgcgctg ctcggcgtgg gcctcaccaa cgtgatcgtg gcggtcggca
     1201 tcagcacgat cccgcggttc gccaggctgg tgcgcagctc cgttctctcc atccgcgagg
     1261 tgacctacat cgaggcctcc atggccctcg gcgtgcccca ccggcgcgtg ctctggcgcc
     1321 acgtcctccc gaacgccctc gcgccggtca tcgtccaggc caccctgagc atgggcgcca
     1381 ccatcctcac ggccgccggg ctcggcttcc tcggcctcgg cgtgcagcca ccgacgccgg
     1441 agtggggtgc catgctcggc gaaggccgca actacatctt ctcgcaaccg tcggtgacga
     1501 cattcccggg gttggccata ttcctggcgg tcgtaggttt caacctactc ggcgacggcc
     1561 tgcgcgacgc gctcgacccg cagttgaggg gtagaaggta gccgaacccg gttcgccatt
     1621 cccgatcgtg ttgggcatcg gcactatact tgagcaaatt actcggttat agtcgctcct
     1681 cacgtggtgg cctccaccgc agcccatcag gagagcgacc ggacggccgc gctcctcaga
     1741 aggagccaaa ccgatgcatc tccgtacgtc tttgggccgg tccctagcgc tgctcatctt
     1801 ctccctcttc gcggccgggg ccgcgttcgc gcaaaccgcc gtcgtgg
//
LOCUS       DAOQYE010000457         1846 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_75360_length_1846_cov_2.644891, whole genome shotgun sequence.
ACCESSION   DAOQYE010000457 DAOQYE010000000
VERSION     DAOQYE010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75360_length_1846_cov_2.644891"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..647
                     /locus_tag="PKN52_07780"
     CDS             <1..647
                     /locus_tag="PKN52_07780"
                     /inference="COORDINATES: protein motif:HMM:NF013705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HNQ99887.1"
                     /translation="RNASPEEFAGFSDFFQSLFGGAGGFGGGRAVYSSDFGGRSVRSD
                     PFDFGEPRPQNVEARLDIGVLDAYRGGPTSIRVGDKSLEVTIPKGTRDGAKLRLRGQA
                     PGGGDLILHVRHLPSPTFTVEGDSVRVKLRVPDYLAALGGSVRVPTLDGEVDMTLPPG
                     SSSGRVLRLRGQGWPTADGRGDEFAEVRVSVPDHLSAEQRRLYEELKGLGRDPS"
     gene            756..1751
                     /locus_tag="PKN52_07785"
     CDS             756..1751
                     /locus_tag="PKN52_07785"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNQ99888.1"
                     /translation="MTRSPAFSRVLKTLAVLATSLLLAGAFAQGESDGAQGAAADDPV
                     VLRLGSYEERASDFAWRFGVTLRGVAAQQGQPYSEDFAASLWSLIPYYLDQRTQEVAL
                     VAEARRRGLVPDAAKLESTLDNVKAGLAEDEDFDTVVRQAGFPNEAGLRSMIEESDLI
                     QQLLTQVHDEAAAGVTDDQVRVRYLAERQRFTQPEQYCAKHILVPEESTARDLLTRLS
                     LGSDFGDLAAEFGTDGTKTRGGDLGCFGLGAMVPDFEAAIVAAPVGENVGPVQTQFGY
                     HLILVYDHTPARVRPLSEVEGSARELVVASASDALLNGIIGGAGVVTYPENLPAF"
BASE COUNT          265 a          668 c          628 g          285 t
ORIGIN      
        1 tccgtaacgc ttcgccggag gagttcgccg gcttctccga cttcttccaa tcgctcttcg
       61 gtggtgcggg cggcttcggc gggggccggg ccgtgtactc gagcgacttc ggcgggcgct
      121 ccgtcaggag cgaccccttc gacttcgggg agcctagacc gcagaacgta gaggcgcggc
      181 tggacatagg cgttctcgac gcctaccgcg gcggtccaac ctcgatccgt gtcggtgaca
      241 agagcctgga ggtcacgatc ccgaagggta cgcgagacgg cgccaagcta aggctccgcg
      301 gacaggctcc cggcgggggc gacctcatct tgcacgtgcg ccaccttcct agccccacct
      361 tcacggtcga aggcgactcc gtgcgggtca agcttcgcgt gccggactac ctggcggccc
      421 tgggtggcag cgtcagggtg ccgaccctgg acggcgaggt cgacatgacc ttgccgccgg
      481 gctcctccag cggccgggtc ttgcggttac gcggtcaggg ctggccgacg gccgacggcc
      541 gcggcgacga gttcgccgaa gtgcgagtca gcgtgccgga ccacctgagc gcggagcagc
      601 ggcggctcta cgaggaactc aaagggttgg gtcgagaccc gagctgaggc gttgcgccgc
      661 cgcgccgcgg gcgcgacccg ccggcgagca tgcgcccact gggcgcgcgc tatggtgcgc
      721 accccagcgg cggcgcaggt gttagcctgc ctcccatgac ccgatccccg gccttcagcc
      781 gcgtactcaa gaccctcgcc gtcctggcga cgtccctcct cctagccgga gccttcgccc
      841 agggcgaaag cgacggcgct cagggcgccg cagcggacga ccccgtcgtc ctccgcctcg
      901 gctcctacga ggagcgcgcc agcgacttcg cttggcgctt cggcgtgacg ctgcgcggcg
      961 tcgccgccca gcagggccag ccttactcgg aggacttcgc ggcgagcctc tggagcctca
     1021 tcccctacta cctcgaccag cgtacgcagg aagtcgcgct cgtggccgag gcgcgtcgcc
     1081 gcggcctcgt gcccgacgcc gccaagctcg agtcgacgct tgacaacgtg aaggcgggcc
     1141 tggcggagga cgaggacttc gataccgtcg tccgtcaggc cggcttcccg aacgaggcgg
     1201 ggcttcgttc gatgatcgag gagtccgacc tcatccagca gctcctcacg caagtgcacg
     1261 acgaggccgc cgccggcgtg accgacgacc aggtgagggt acgctacctg gccgagcgtc
     1321 agcgcttcac gcaacccgag cagtactgcg ccaagcacat cctcgtgccg gaggagtcga
     1381 cggcgcgcga cctgctcacc aggctctcgc tcggctccga cttcggcgac ctagccgcgg
     1441 agttcggcac cgacggcacg aagactcgcg gcggcgacct cggttgcttc ggacttggcg
     1501 ccatggtgcc cgacttcgag gcggccatcg tggccgcgcc cgtcggcgag aacgtcggcc
     1561 cggtgcagac ccagttcggt taccacctca tcctcgtcta cgatcacacg ccggcccgcg
     1621 tccggccgct cagcgaggtc gagggctcgg cccgcgagct ggtcgtagct tccgcttccg
     1681 acgccctgct taacggcatc atcggcggcg cgggcgtggt cacctacccg gagaacctgc
     1741 cggcctttta ggtcgcccgc tgtaagaggc ccgtcagcgt cccgctccta atgtgatggg
     1801 gcgcgctgga ggcgagacgc attggccttc cccttcccgc tgcgcc
//
LOCUS       DAOQYE010000458         1844 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_75489_length_1844_cov_2.984908, whole genome shotgun sequence.
ACCESSION   DAOQYE010000458 DAOQYE010000000
VERSION     DAOQYE010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75489_length_1844_cov_2.984908"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(177..1781)
                     /locus_tag="PKN52_07790"
     CDS             complement(177..1781)
                     /locus_tag="PKN52_07790"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNQ99889.1"
                     /translation="MSDDTIGWILLSLVAGLATGDAVTTGSVLSTVGSVFAFMVVSFT
                     LGRWLVRQALAFVQDRVVSPNRLLTLVMVLTLAWAAVSQALHIEAVLGAFVMGVLFGQ
                     MRRLPDEVHDRIEGMSVGIFSPIFLAVAGLKVDLRSLLEPRLLLVVLGVIVIATLGKV
                     AGTYLGARLVGRHGHWTALAYGAGLNARGAMGIIMAAIGLQLGIIGQDMYSIIVVMAM
                     TTSIMAPTALRFVLARVKPDEEEARRLRQEELAEDSLIANVHRVLFPVRGPRPGQSKP
                     APRTIEAFVLDKLGKDLSLTLLNVARPGEKVTAQTFLGELATGFDNGEVTRRVVESEA
                     PVDAILDEAAKDYDLIVLGASEQAGTNEVVFNRVVDQVVRMAPCPTMVVKGAGEPVSW
                     PPRRILVPSDGSAAGRHAAELAFALASDAAVHVALLNVAVQQPGPYRMDASGAAEERQ
                     LAAANQIVDNLRQLGEARGVKPSTEVRVAADPVSAILAVAKQLEYDLIVLGTDVRPGS
                     ERLYLGPRVERILAAAEVPVIVINGS"
BASE COUNT          313 a          629 c          605 g          297 t
ORIGIN      
        1 ccggctcctc gagtacggcg ccgtgcgccg caggcgcgcg cccatcaccg tcaacaccga
       61 gcggctgcag gcgttcctcg acgaggcgag cacctgaggc gctgccgagg atcgctgctc
      121 ggaaggggcg tcgggttttc aggaagcctg ccgcccctct tcagtgcgga ccgctctcag
      181 gagccgttga tgacgatgac cggcacctcc gctgcggcca ggatgcgctc tacgcgcggc
      241 ccgaggtaga gccgctccga gccgggacgc acgtcggttc cgaggacgat caagtcgtat
      301 tcgagctgct tggctacggc gaggatcgcc gagaccgggt ccgccgcgac gcgcacctca
      361 gtgctcggct tcacgccgcg tgcctcaccg agttggcgca ggttgtcgac gatctggttg
      421 gctgccgcta gctgacgctc ctcggcggcg ccgctcgcat ccatgcggta cggtccgggc
      481 tgttgcacgg ccacgttgag tagggctaca tgcaccgctg cgtccgacgc cagcgcgaag
      541 gcgagctcgg cagcatgccg gccggcagcc gagccgtcgc tcggcactag gatgcgtcgc
      601 ggcggccagg agaccggctc cccggcaccc ttcacgacca tggtggggca gggcgccata
      661 cgcacgacct gatcgaccac gcggttgaag acgacctcgt tcgtgccggc ctgctcggaa
      721 gcgccgagta cgatgaggtc gtaatccttc gccgcctcgt ccagtatcgc gtcgacgggc
      781 gcctccgatt cgaccaccct gcgggttacc tcgccgttgt cgaaaccggt ggcgagctcg
      841 ccgaggaacg tctgggccgt gaccttctcg ccaggcctgg cgacgttgag cagtgtcagc
      901 gacaggtcct tgccgagctt gtcaagcacg aaggcctcga tggttctcgg cgcaggcttg
      961 ctttggccgg gtcgtgggcc acgcacggga aacaagacgc ggtggacgtt ggcgatgagg
     1021 ctgtcctccg ccagctcctc ctggcgtagc ctgcgcgcct cctcctcgtc cggcttcaca
     1081 cgggcgagca cgaagcgcag cgcggtcgga gccatgatgg acgttgtcat cgccatgacc
     1141 acgatgatcg aatacatgtc ctggccgatt atgcctagtt ggagcccgat ggcggccatg
     1201 atgatgccca tggccccgcg ggcgttcagc ccagccccat aggcgagcgc cgtccagtga
     1261 ccgtgtcggc ccaccagccg ggcgcccagg taagtaccgg cgaccttgcc aagggtggcg
     1321 atgacgatga ctccgaggac cactaggagc aatctcggtt ccagcagact gcgcaggtct
     1381 accttgagac ctgccacggc caggaagatg ggcgagaaga tccctacaga catgccttcg
     1441 atgcggtcgt gcacctcgtc gggcaggcgt cgcatctgtc cgaacagcac gcccatgacg
     1501 aacgctccga gcaccgcctc gatgtgcagc gcctggctca ccgccgccca cgcgagggtg
     1561 agaaccatga cgagcgtcag gaggcggttc gggctgacca cccggtcctg tacgaaggcg
     1621 agagcctgac gaaccaacca ccgccccaaa gtgaacgaca ccaccatgaa cgcgaacacg
     1681 ctcccaacgg tgctgagaac gctgccagtc gtcacggcgt cgcctgttgc caggccggcc
     1741 accagcgata ggaggatcca gccgatggtg tcatcgctca tgctggatcc tcccaacggt
     1801 gctgagaacg ctgccagtcg tcacggcgtc gcctgttgcc aggc
//
LOCUS       DAOQYE010000459         1844 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_75510_length_1844_cov_2.378983, whole genome shotgun sequence.
ACCESSION   DAOQYE010000459 DAOQYE010000000
VERSION     DAOQYE010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75510_length_1844_cov_2.378983"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="PKN52_07795"
     CDS             complement(<1..236)
                     /locus_tag="PKN52_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HNQ99890.1"
                     /translation="MSFSSTVIDYGAGNLRSIERALGAAGLSPRVVSDPADVGRPDLL
                     VLPGQGHFGQVARAFRASGFEAVVREHVAAGRAFL"
     gene            complement(233..835)
                     /gene="hisB"
                     /locus_tag="PKN52_07800"
     CDS             complement(233..835)
                     /gene="hisB"
                     /locus_tag="PKN52_07800"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HNQ99891.1"
                     /translation="MDQTRTAEVVRETRETRIRVSVDLGATNDASTGPARLGTGHGFL
                     DHLLEQLARHGRFALSLEGTGDLHVDVHHMAEDCGIVIGQAFSEALGSRQGIERYGSA
                     FVPMDETLAHVVVDLSGRPHITFDPGTLHGDSGGFTAYHLREFLRGFANHAGATLHVR
                     LLAADDTHHACEAVMKAFARALRDAVKITHADLPSTKGLL"
     gene            complement(856..>1844)
                     /locus_tag="PKN52_07805"
     CDS             complement(856..>1844)
                     /locus_tag="PKN52_07805"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HNQ99892.1"
                     /translation="PDDVPAALKERVMRALLDVAWNRYPGLGPTDLEARLAEHHGVAT
                     ESVVVANGSNTLIQALTIVSGIGRSVVTVAPTFAVYASQARLLGAELVEVPLGPRFAL
                     PARELRDALTNRSGVLFLANPAAPTGNLFGEAEVEDLIGAAGEFLVVIDEAYSEFAGR
                     NLEALAARQENVVLLRTFSKAFGLAGARVGYALAHAGVAEELRKALLPFSVSAWQTAT
                     AMALLDEREVVAQRVHHVLSERGRVWAALAAMPGVEPFPSEANFILFRVTDPAAWYRS
                     LLEAGVVIRRQDHLPGAEGCLRVSIGTVSENDAFLAACEAASSAARSVAPPA"
BASE COUNT          278 a          631 c          681 g          254 t
ORIGIN      
        1 aggaacgcgc ggccggcagc aacgtgctcg cgtacgaccg cctcgaaccc cgacgccctg
       61 aaggctcgcg ccacctgccc gaagtggcct tggccaggga ggacgagcag gtccggtcgg
      121 ccgacgtcgg ccgggtcgga gaccacccgg ggcgagagcc cagcggcccc gagggcgcgc
      181 tcgatgctcc tgaggttgcc tgccccgtag tcgatgaccg tgctgctgaa gctcacagga
      241 ggcccttggt ggagggtagg tcggcatggg tgatcttgac cgcatcgcgc agggcgcgtg
      301 cgaaggcctt catgaccgcc tcgcacgcgt ggtgcgtgtc gtcggcggcc aggaggcgta
      361 cgtggagcgt cgccccggca tggttggcga agcccctgag gaactcgcgc agatggtagg
      421 cggtgaaccc gcccgagtcg ccgtgcagcg tgccagggtc gaaggtgatg tggggccggc
      481 cggacaggtc gaccacgacg tgggccagcg tctcgtccat cgggacgaac gcgctgccgt
      541 agcgctcgat cccctgacgg cttccgagtg cctccgagaa cgcctgaccg atgacgatac
      601 cgcagtcctc cgccatgtga tggacgtcga cgtgcaggtc gcccgtgccc tcgagggaga
      661 gcgcgaagcg gccgtggcgc gcgagctgct ccaggaggtg gtcgaggaag ccgtggccgg
      721 tgccgaggcg cgccggtcca gtggacgcgt cgttggtcgc gcccaagtca acggacacgc
      781 ggatgcgcgt ctcgcgcgtc tcgcgaacga cctcggcggt gcgtgtctgg tccatctcgt
      841 tcctccaaag gttggtcatg cggggggcgc gacgcttcgc gcggcgctcg aggcggcctc
      901 gcaagcggcc aagaaggcgt cgttctccga cacggtgccg atggataccc ttaggcaacc
      961 ctcggcgcct ggcaggtggt cctggcgcct gatcacgacg ccggcctcga ggaggctgcg
     1021 gtaccaggcc gccggatccg tgacccggaa caggatgaag ttcgcctcgg aggggaaggg
     1081 ctccaccccg ggcatggcgg ccagagcggc ccaaacgcgg ccccgctcgc tcagcacgtg
     1141 gtgcactcgc tgcgccacca cctcgcgctc gtcgagcagc gccatggccg tcgcggtctg
     1201 ccacgcgctg acgctgaacg gcagcaacgc cttgcggagc tcctccgcga cgccagcgtg
     1261 cgccaacgcg taaccgacgc gcgcgccggc gagcccgaac gccttgctga aggtgcgcag
     1321 gagcacgacg ttctcctggc gcgccgccag ggcctcgagg ttcctgcccg cgaactcgga
     1381 gtacgcctcg tctatcacga ccaggaactc acccgccgcc ccgatcaggt cctccacctc
     1441 ggcctcgccg aagaggttgc cggtcggagc ggccgggttg gcgaggaaca gcacgccgga
     1501 gcggttcgtg agcgcgtcgc gcagctcacg ggccggcagc gcgaagcgag ggccgagggg
     1561 cacctcgacg agctcggctc cgaggaggcg cgcttgcgac gcgtagacgg cgaacgtcgg
     1621 cgcgaccgtg acgacgctgc gcccgattcc ggacacgatg gtgagggcct ggatgagggt
     1681 gttcgagccg ttggccacga cgacggactc ggtggccacg ccgtggtgct cggccaggcg
     1741 cgcctcgagg tccgtcgggc ccaaaccggg atagcggttc cacgcgacgt ctaggagcgc
     1801 gcgcatgacg cgctccttga gcgccgcggg cacgtcgtcc gggc
//
LOCUS       DAOQYE010000460         1843 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_75546_length_1843_cov_3.281879, whole genome shotgun sequence.
ACCESSION   DAOQYE010000460 DAOQYE010000000
VERSION     DAOQYE010000460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75546_length_1843_cov_3.281879"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..885)
                     /locus_tag="PKN52_07810"
     CDS             complement(<1..885)
                     /locus_tag="PKN52_07810"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNQ99893.1"
                     /translation="MPRTEEPNPPPQGTRRGVVMAFLMLAMSLAAIEGTIVATAMPSI
                     VASVGGFEYYAWVFAAFLLAQATSTPLYGRLADAHGRKPILLLGIAVFMLGSGLCGFA
                     TTMPLLIAFRVLQGFGAGAVYPMVTTLVGDLYEVRDRGKAQAYLSSVWGVSAIAGPLL
                     GGALVQHAHWGWVFWFNVPFGLAAILGLGAYLRESVRRTESRIDVGSAALMFVAIGGL
                     MLFLTQGALWGTAAAVTLVVIAGAAGAGFVRRQLRSGAPLIGLDLWRDPLILLADATT
                     FMGGMLMIGLITYSPTYVQ"
     gene            complement(914..>1486)
                     /locus_tag="PKN52_07815"
     CDS             complement(914..>1486)
                     /locus_tag="PKN52_07815"
                     /inference="COORDINATES: protein motif:HMM:NF015552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance/C4-dicarboxylate
                     transporter family protein"
                     /protein_id="HNQ99894.1"
                     /translation="DAASLGNTALPDAGAGLLFTGLCLFLVGAVLYLLIVGLIFYRFT
                     FLKLEASQLAAPYWINMGALAITTLTGATLLAQPGPEPLWSMIAPALLGFTLLFWAFG
                     SWWVPLLVVFGVWRHAVAKYPLRYAPEYWGLVFPLGMYSVASRRLADHIRIPVLEHVS
                     SAFAWVALAAWAVAFLGMLGALLPFRRTIT"
     assembly_gap    1487..1496
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1497..>1843)
                     /locus_tag="PKN52_07820"
     CDS             complement(<1497..>1843)
                     /locus_tag="PKN52_07820"
                     /inference="COORDINATES: protein motif:HMM:NF015552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99895.1"
                     /translation="LACAFAARFALARQRMTQEFVDHARGPGYFTWVAATCVLASQVS
                     GFLGADQVARGLLAFAAILWVSVTYGFFAAVTLRRTKPRLESALSGSWLLAVVATQAL
                     GLLAAQVGGSATD"
BASE COUNT          352 a          612 c          657 g          212 t
ORIGIN      
        1 ctggacgtaa gtcggcgagt acgtgatgag gccgatcatg agcatgccgc ccatgaaggt
       61 cgtggcgtcg gccaggagta tgagagggtc gcgccacagg tcgagcccga tgagcggagc
      121 gccgctgcgg agctggcgcc gcacgaaccc ggcgcccgcg gcgccggcga taacgacgag
      181 cgtgaccgcg gccgcggtcc cccacaaggc gccttgcgtc aggaagagca tcaggccgcc
      241 gatggcgacg aacatgagcg cggcactgcc gacatcgatg cggctctcgg tacgcctcac
      301 ggattcgcgc aagtaggcgc caagccccag gatggcggcc agcccgaagg gcacgttgaa
      361 ccagaagacc cagccccaat gcgcgtgctg cacgagcgcg ccgcccagca gcggccccgc
      421 gatggccgag acgccccaca cgctggagag gtaggcctgg gccttacccc ggtcgcggac
      481 ctcgtagagg tcgccgacga gggtggtgac catcgggtag acggcgcccg ccccgaaacc
      541 ctgcaggacc ctgaacgcga tgagcagcgg catggtcgtc gcgaagccgc atagaccgga
      601 gcccagcatg aacacggcta tgccaaggag gagtatgggc ttgcgcccat gggcgtcggc
      661 cagccgaccg tacaggggcg tagacgtcgc ctgggcgaga aggaacgcgg cgaacaccca
      721 ggcgtagtac tcgaagccgc cgacgctcgc gacgatgctt ggcatggccg tcgccacgat
      781 cgtgccctct atggcggcga gcgacatggc gagcatcagg aaggccatca ccacgccgcg
      841 cctcgtgccc tggggcggtg ggttcggctc ctcggttcgc ggcatgtgca tcagatgata
      901 tgccgcgtgg ttctcaggtg atcgtcctcc tgaaaggcag cagcgccccg agcatgccga
      961 ggaacgcgac cgcccacgcc gccagcgcca cccaggcgaa ggcagatgac acgtgctcca
     1021 agacggggat ccggatgtgg tccgcgagtc ggcgcgaggc gacgctgtac atccccagcg
     1081 ggaagacgag cccccagtac tccggggcgt agcgcagggg gtacttggcc acggcgtgac
     1141 gccatacccc gaagacgacc agcaggggca cccaccaact gccgaacgcc cagaagagga
     1201 gggtgaagcc gaggagcgcc ggggcgatca tgctccagag cggctcggga cccggctgcg
     1261 ccagcagcgt cgcgcctgtc agcgtcgtta tggcaagcgc gcccatgttg atccagtacg
     1321 gggcggccag ttggctggcc tcgagcttca ggaaagtgaa ccggtagaag atcaggccga
     1381 cgatgagcag gtataggacg gcgcctacga ggaacaggca taggcccgtg aaaagcaggc
     1441 cggcgcccgc gtccggcagg gccgtgttcc caagcgaagc ggcgtcnnnn nnnnnngtcc
     1501 gtggcactcc cgcccacctg cgccgccagc aggcccaagg cctgcgtggc cacgacggcg
     1561 agcagccaag agccgctcaa cgcactctcg aggcggggct tcgtgcgccg caacgtcacg
     1621 gcggcgaaga agccgtaggt cacgctaacc cacaggatgg ccgcgaacgc cagcaggccg
     1681 cgagcgacct ggtcggcgcc gaggaagccg gagacctggc ttgccaggac gcaggtcgcc
     1741 gccacccacg tgaagtagcc ggggccgcgg gcgtggtcga cgaactcctg ggtcatccgc
     1801 tgccgcgcga gcgcgaaccg cgcggcgaag gcgcaggcca ggg
//
LOCUS       DAOQYE010000461         1841 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_75686_length_1841_cov_3.199888, whole genome shotgun sequence.
ACCESSION   DAOQYE010000461 DAOQYE010000000
VERSION     DAOQYE010000461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1841
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75686_length_1841_cov_3.199888"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1622)
                     /locus_tag="PKN52_07825"
     CDS             complement(<1..1622)
                     /locus_tag="PKN52_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ99896.1"
                     /translation="MGFIMDGLSAEGYDREYTDGQLVRRIVKYFRPYLGIMLVVGLAV
                     LLAAALDAALPVLVSAGIDSLAAAEDMRAELWGRVGWLVAAFAVAGALSWGLNFLRQW
                     LTARAVGDVVLALRYDAFDAVMQRDMSFYDEFSTGRIVSRVTSDTQDFSNVVTLTLNL
                     ISQVLQVVILLGILLYIDARLALIALAIAPVVIAIALAFRRIARVVTVQARRVIAEVN
                     ANVQESLTGISVAKTFRQEHAVYRLFLGVNNQSYRLNLLQGYIFSAIFPVLGIVGGIG
                     TALIVYFGGLRALGGSITAGEWFLFVQVVGLFWFPLTSIASFWSQFQLGLSASERVFA
                     LIDAEARVVQTDSVKVDRVAGRIQFEGVDFGYNDQEPVLQGFDLTIEAGETVALVGHT
                     GAGKSSIGKLVARFYEFQGGRLTIDGQDIRALDLASYRHHLGVVQQTPFLFSGTVRDN
                     IRYGKEDATDEEVLAVAARIGGGDWVDALPNGLDTDVGEGGRGVSMGQRQLVAMARVL
                     LQDPSILILDEATASVDPLTEAQIQEGLDEVLA"
     gene            complement(1622..>1841)
                     /locus_tag="PKN52_07830"
     CDS             complement(1622..>1841)
                     /locus_tag="PKN52_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ99897.1"
                     /translation="SAIDSATEDAIQRAINAVLAGRTTLMITHRLAQIRRADLVLLMH
                     GGALVARGSHHELLASSDLYRRIFARQD"
BASE COUNT          306 a          603 c          654 g          278 t
ORIGIN      
        1 gccaggacct cgtcgaggcc ctcctggatc tgcgcctcgg tcagcgggtc gacgctggcc
       61 gtcgcctcgt cgaggatcag gatgctcggg tcctgcagga gcacccgcgc catggcgacg
      121 agctggcgct gtcccatcga cacgccgcgg ccgccctccc ccacgtccgt gtcgagcccg
      181 ttcggtaggg cgtctaccca gtcgccccca ccgatgcgcg ctgccacggc cagtacctct
      241 tcgtccgtcg cgtcctcctt cccgtaacgg atgttgtcac gcaccgttcc gctgaagagg
      301 aacggcgtct gctggacgac gccgaggtgg tgccggtagc tcgccaggtc gagagcgcgg
      361 atgtcttgcc cgtcgatggt caggcgcccg ccctggaact cgtagaagcg cgccacgagc
      421 ttgccgatgc tcgacttgcc cgcgccggtg tggccgacga gcgcgacggt ctcgcccgcc
      481 tcgatcgtga ggtcgaaccc ctgcaggacg ggctcctggt cgttgtaacc gaagtcgacg
      541 ccctcgaact ggatgcgccc ggcgacccgg tcgaccttga ccgagtccgt ctgcacgacg
      601 cgcgcctcgg cgtcgatgag ggcgaagacg cgttcgctgg cggagaggcc gagttggaac
      661 tggctccaga acgacgcgat cgaggtgagc gggaaccaga agagcccgac gacctgcacg
      721 aacaggaacc attcgcctgc cgtgatgctg ccgccgaggg cgcgcaggcc gccgaagtac
      781 acgatcagag cggtgccgat gccgccgacg atgccgagca ccgggaagat ggccgagaag
      841 atgtagccct gcaggaggtt gagacggtag gactggttgt tgacgcccag gaacaggcgg
      901 tagacggcgt gctcctgcct gaaggtcttg gcgaccgaga tgcccgtgag cgactcctgc
      961 acgttcgcgt tcacctcggc gatcacgcgc cgcgcctgca cggtcacgac gcgcgcgatg
     1021 cgcctgaacg cgagcgcgat ggcgatgacg acgggcgcga tggcgagggc gatgagcgcg
     1081 aggcgcgcgt cgatgtagag caggatgccg agaaggatga caacctgaag gacctggctg
     1141 atgaggttga gggtgagcgt gacgacgttc gagaagtcct gcgtgtcgga cgtgacgcgg
     1201 ctgacgatgc ggccggtaga gaactcgtcg tagaaagaca tgtcgcgttg catgacggcg
     1261 tcgaaggcgt cgtagcgcag cgcgagtacg acgtctccca cggcgcgtgc ggtgagccac
     1321 tgccgtagga agttgaggcc ccacgacagg gcgcccgcca ccgcgaacgc ggccacgagc
     1381 cagcccaccc gcccccatag ctccgcgcgc atgtcctcgg ccgccgccag gctgtctatg
     1441 ccggcggaga cgagcacggg cagggcggcg tcgagcgccg cggccaggag cacggccagt
     1501 ccgaccacca gcatgatgcc gaggtacggc ctgaagtact tgacgatgcg cctcacgagc
     1561 tggccgtccg tgtactcgcg gtcgtacccc tcggccgaga ggccgtccat gatgaagccc
     1621 atcagtcctg cctcgcgaag atgcgccggt agaggtcgct actggccagg agttcgtggt
     1681 ggctgccccg cgccaccagg gcgccgccgt gcatcaggag cacgaggtcg gcgcggcgga
     1741 tctgcgccag gcgatgcgtg atcatcaggg tggtcctgcc tgccagaacg gcgttgatgg
     1801 cgcgctggat ggcgtcttcc gtggcgctgt cgatggcgct g
//
LOCUS       DAOQYE010000462         1839 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_75813_length_1839_cov_3.144058, whole genome shotgun sequence.
ACCESSION   DAOQYE010000462 DAOQYE010000000
VERSION     DAOQYE010000462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1839
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75813_length_1839_cov_3.144058"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1225
                     /locus_tag="PKN52_07835"
     CDS             <1..1225
                     /locus_tag="PKN52_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99898.1"
                     /translation="VLGTFLLFTHGSVLVLRLLRSAKTSYLRGTRMLVVSQLVFRVRD
                     NARLLATIATLSAVVLAAAGSFYVFNRGFEQGLEGMYQQELAFIERSDGRPSLGPSVV
                     DGVFVRNEVAPTVRVSVRIHELRFRDQPAEVGDGWLVLVGASDYAALARAAGFAAGFA
                     AETAAGTAAQAAAPAPTSLRLSDGVAVLGGGGIAVSRVAPPVTMPPSLQGPWYVIDDP
                     ALADLPTGGREYAPAKFWLYDWPESAATRRLESELQEAVLAAAPRQDVDARFLGVRQI
                     RQTLGLSMFAGVFVSLLFFIGAGSLIYFKLFTELGEDRRLHGRLRRIGVTPGESAKVV
                     TAQIAVIYLLPFALGAVHAAFALDALGSLLQQDVTRYSLVVIGLFAAVQGAFFLLTRW
                     TYLRALRPEAVAVRA"
     gene            1351..>1839
                     /locus_tag="PKN52_07840"
     CDS             1351..>1839
                     /locus_tag="PKN52_07840"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: protein motif:HMM:NF015319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HNQ99899.1"
                     /translation="MTIEPGDSSTRPGWAEQDDIMRAYYDDEWGVPVTDERELFEALS
                     LEVFQTGLSWSTVLRKRPAFRAAFADFRPDVVAAYQEVDVVRLLSDAGIVRNEQKIRA
                     TIRNAAATMALRDRGGLVELVWSFRTDPEAAGSEGSRTRSPESEALAKALRGHGFSFV
                     GPT"
BASE COUNT          232 a          621 c          654 g          332 t
ORIGIN      
        1 ggtgctgggc acgttcctcc tcttcacgca tggcagtgtc ctggtgctgc gcctgctgcg
       61 ctccgccaag accagctatt tgaggggcac gcgcatgctg gtcgtgtcgc agctcgtctt
      121 ccgggtgcgc gacaacgccc gtctgctcgc caccatcgcg accttgagtg ccgtcgtgtt
      181 ggccgccgcc gggtcgttct acgtcttcaa ccgcggcttc gagcagggcc tcgaaggcat
      241 gtaccaacag gagctggcgt tcatcgagag gtccgacggc aggccgagcc tcggtccgtc
      301 cgtggtggac ggcgtctttg ttcgcaacga ggtcgcgccg acggtgaggg tttccgtgcg
      361 catccacgag ctgcggttcc gcgaccagcc tgccgaggtc ggcgacggtt ggctcgtgct
      421 cgtcggcgcg agcgactatg cggccctcgc ccgggcggcg gggttcgcgg cggggttcgc
      481 ggccgagacc gctgccggga ccgcggctca ggccgccgct cccgctccca cgtcgctgcg
      541 cttgtccgat ggcgtggcgg tattgggagg cggcggcatc gcggtttccc gcgttgctcc
      601 gccggtgacg atgcccccga gcctgcaggg cccctggtac gtgatagacg accctgcgct
      661 cgcggacctg ccgaccgggg ggcgcgagta cgccccggcc aagttctggc tgtacgactg
      721 gccggagagt gcggcgacgc ggcgcctcga gtctgagctg caggaggcgg tcctggcggc
      781 ggcgccccgc caggacgtcg atgcccgctt ccttggcgtg cgccagatca ggcagacgct
      841 cgggctgtcg atgttcgccg gcgtgttcgt ctccctactc ttcttcatcg gggcgggcag
      901 cctgatctac ttcaagctgt tcaccgaact gggtgaggac cgccgtttgc acgggcgatt
      961 gcgcaggatc ggcgtgacgc cgggcgagtc ggcgaaggtc gtcaccgcgc agatcgcggt
     1021 catctacctg ctgcccttcg cactcggcgc ggtccacgcg gccttcgcgc tcgacgcgct
     1081 cggcagcctc ctgcagcagg acgtcacgcg ctacagcctg gtggtcatcg gcctcttcgc
     1141 cgcggtgcag ggtgccttct tcctgctcac gcgctggacg tacctgcgcg ccctgcggcc
     1201 ggaggccgtg gctgtgcgtg cgtaggcgtg ctcctaggag gagaccctcc gagactgcgg
     1261 catcggccgc ggctgacgcc gaggaagatg ccgagcgcac ataggctccc ggtcatgttc
     1321 gcgcccgccg tccccgtata gccttggcgc atgaccatcg aaccgggcga ctcctcaacc
     1381 agacctggct gggccgagca ggacgacatc atgcgggcgt actacgacga cgaatggggc
     1441 gtgcccgtca cggatgagcg cgagctgttc gaggcgctga gcctcgaggt gtttcagacg
     1501 ggtctctcgt ggtcgacggt cctgcgtaag cgcccggcgt tccgggccgc gttcgccgat
     1561 tttcggcccg acgtggtcgc cgcgtatcag gaagtcgacg tggtccgcct gctttctgac
     1621 gccgggatcg tcaggaacga gcagaagatc cgcgccacga tccgcaacgc ggcggcgacg
     1681 atggcgctac gcgaccgtgg cgggttggtg gaactcgtct ggtcttttcg gaccgaccct
     1741 gaggcggccg gctccgaggg gtcacgcacg cgctcgcccg agtctgaggc gctcgccaag
     1801 gctctgcgcg ggcatggctt cagcttcgtg ggtccgacg
//
LOCUS       DAOQYE010000463         1838 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_75881_length_1838_cov_3.049355, whole genome shotgun sequence.
ACCESSION   DAOQYE010000463 DAOQYE010000000
VERSION     DAOQYE010000463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75881_length_1838_cov_3.049355"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1121)
                     /gene="tig"
                     /locus_tag="PKN52_07845"
     CDS             complement(<1..1121)
                     /gene="tig"
                     /locus_tag="PKN52_07845"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HNQ99900.1"
                     /translation="MEARVVEKQGVNVTLSVRVDAATVDATFERVLAQLSRTVKVPGF
                     RPGKAPRGVLEKRVGVEALAEEVRDALVEEAYPQAVRELELYPIDAHFHADGAVRGQD
                     FEFTVHADVYPDITLPDLEAIKLEAEPKVVSDDDVRSAFEELRRENATLVPVDRPVEA
                     TDWVLVARLGDDDEADESSTFQVHMDRAGEELKSQLIGKSMGDVVTATFTDPAPEGEE
                     GTTTTQIRLKVLDVKGKELAATDEDLALQLGFATGEELTQRVRESLESDARRATLEAR
                     RDEVVEKLVAGSTLDLPGSLVRRRQRSLLQDLAHDLSHQGTTLERYVDRLEQRGKREE
                     FEAELKEAAEKGVRRDLVLERLMEVRGTELSDAEYRSAL"
     gene            complement(1221..1304)
                     /locus_tag="PKN52_07850"
     tRNA            complement(1221..1304)
                     /locus_tag="PKN52_07850"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1268..1270),aa:Leu,seq:tag)
     gene            complement(1409..1485)
                     /locus_tag="PKN52_07855"
     tRNA            complement(1409..1485)
                     /locus_tag="PKN52_07855"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1449..1451),aa:His,seq:gtg)
     gene            complement(1565..>1838)
                     /locus_tag="PKN52_07860"
     CDS             complement(1565..>1838)
                     /locus_tag="PKN52_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99901.1"
                     /translation="AVAFAGILANGGLPLMVLAAQDAAPHAVATASGMMMGLTWGTAG
                     LLYLVVGAVQEAIGMNAAMWLSVVPLLPGALVAYRVLKGLEAGAQA"
BASE COUNT          273 a          646 c          632 g          287 t
ORIGIN      
        1 ttcagggcgg aacggtactc ggcgtccgac agttccgtgc cacggacctc catgaggcgt
       61 tccagcacga ggtcgcgccg cacgcccttc tcggccgctt ccttgagctc cgcctcgaac
      121 tcctcgcgtt tgccgcgctg ctcgaggcgg tcgacgtagc gctccagcgt ggtgccctgg
      181 tggctcaggt cgtgggccag gtcctgcagg aggctgcgct ggcggcgcct gacgaggctg
      241 cccggcaggt cgagggtcga gccggcgacg agcttctcca cgacctcgtc gcggcgagcc
      301 tccaaggtgg cgcgcctggc gtcggactcc aggctctcgc gcacacgctg cgtgagctcc
      361 tcgccggtcg cgaacccgag ctggagcgcc aggtcttcgt ccgtggcggc gagctccttg
      421 cccttgacgt caaggacctt gagccggatc tgcgtggtgg tggtgccctc ctcgccctcc
      481 ggcgcggggt cggtgaacgt ggcggtcacc acgtcgccca tagacttgcc gatgagctgg
      541 ctcttcagct cctcgccggc acggtccatg tgcacttgga aggtcgacga ctcgtcggcc
      601 tcgtcgtcgt cgcccaagcg cgccaccagg acccagtcgg tagcctcgac cggccggtcg
      661 accggcacga gcgtggcgtt ctcccggcgc aactcctcga acgcggagcg cacgtcgtcg
      721 tcgctgacga ccttcggctc cgcctcgagc ttgatcgcct cgaggtcggg gagcgtgatg
      781 tcggggtaga cgtcggcgtg gacggtgaac tcgaagtcct gaccgcgcac ggcgccgtcc
      841 gcgtggaagt gggcgtcgat ggggtagagc tcgagctcgc gcacggcctg cgggtaggcc
      901 tcctcgacga gggcgtcgcg gacctcctcg gccagggcct cgacgccgac gcgcttctcc
      961 agcacgccgc gcggggcctt gcccgggcgg aagccgggca ccttcaccgt ccgcgagagc
     1021 tgcgcgagca cgcgctcgaa ggtggcgtct accgtggcgg cgtcgacgcg cacgctgagc
     1081 gtgacgttga ccccctgctt ctcgactact ctggcttcca tgacactcct aatcggcttg
     1141 ggtagcgccg caccaacgag ccgctgaacg ggcgcccgca gggcgccaaa aggtagcggc
     1201 aagcgcttgg cttgccgcgg tggtgcgagg agcgggactt gaacccgcac ggctaggcca
     1261 ctagatccta agtctagcgc gtctaccagt tccgccatcc tcgcgcgcag atgcccgcgg
     1321 tccccctaca acttcgccag cgcggaaagt aagggagggc gggccgaact gctcgagacc
     1381 cgccctcttc gcgggagacc ccgccggctg gggtggatga tgggatttga acccacgaca
     1441 cccggaacca caatccggtg ctctaaccag ctgagctaca cccaccaagc aggcgcaaac
     1501 gaacccaaga ggttcgcgag gccgaaccga aggttaccgc gtgggaggcg ggcttggcta
     1561 gtcctcaggc ctgtgcgccg gcctccagcc ctttgagcac ccggtacgcc acgagcgctc
     1621 ccggcaggag cggcactacg ctcagccaca tggccgcgtt catgccgatg gcttcctgga
     1681 ctgccccgac gacgaggtag agcaggccgg ccgtacccca cgtgaggccc atcatcatgc
     1741 cggaggccgt ggcgacggcg tgcggcgcgg cgtcctgggc ggccagcacc atgagcggca
     1801 ggccgccgtt cgcgaggatg ccagcgaagg cgacggcc
//
LOCUS       DAOQYE010000464         1838 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_75904_length_1838_cov_2.280987, whole genome shotgun sequence.
ACCESSION   DAOQYE010000464 DAOQYE010000000
VERSION     DAOQYE010000464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75904_length_1838_cov_2.280987"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..563
                     /locus_tag="PKN52_07865"
     CDS             <1..563
                     /locus_tag="PKN52_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99902.1"
                     /translation="LPVTYIAVPGNHARRSPKMQWKLPTETGDWLIAEMVGDLCRENE
                     RIQCVSPKSWSVNVEIRDHVHALNHGTTMAKGGFGGISWYSIQRSDGKLTALESARGR
                     HVNFRWLGHIHQRAEIPAMDGEGELFVVGSLKGGDEYALYGLQSYSGPRQQLVGCHEK
                     TGVSWRYPLFVQFGDERPSRYEDLLS"
     gene            560..811
                     /locus_tag="PKN52_07870"
     CDS             560..811
                     /locus_tag="PKN52_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99903.1"
                     /translation="MSTDAYPEWPCPYCGHEAWSELFAKPMRRCERLNPVICGHCNVL
                     FNANWVDGCWMGRMTEAEWPGSLADFVEMKAEALRLELS"
     gene            808..1578
                     /locus_tag="PKN52_07875"
     CDS             808..1578
                     /locus_tag="PKN52_07875"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HNQ99904.1"
                     /translation="MTYTIHHGDCLEVMAGMPDASVDFIFTDPPYGHNNNNGDLIHRW
                     EAALGRLPDGANPPLARPIANDGAEADEVFAAALPHFRRLLRTGGCCCCCCCCGGGGP
                     DPMFARWSLALDRELEFKQMVVWDKGPMGMGWHYRRSYESVLVAMKPGGPCKWYDDTD
                     RVENIIRPHYKGIRKIIPSADEHPTPKPVGLAAHFIGLHTQPGDVVLDPFMGAGSTGV
                     AAQNLGRDFIGIEIDQHWYGVAEQRLATAAAQLTLATG"
     gene            1588..>1838
                     /locus_tag="PKN52_07880"
     CDS             1588..>1838
                     /locus_tag="PKN52_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99905.1"
                     /translation="MRLITVQERYERGQTFRLVHGDCLEVMAGMEAGSVDAVVTDPPY
                     GLAFMGKHWDHGIPGVDFWTEALDVMKPSAYLLAFGGTRT"
BASE COUNT          307 a          630 c          613 g          288 t
ORIGIN      
        1 acctgcccgt gacctacatc gcggtaccgg ggaaccacgc ccggcgcagc ccgaagatgc
       61 agtggaagct gcccaccgag acgggggact ggctgatcgc ggagatggtc ggcgacctct
      121 gccgggagaa cgagcgcatt cagtgcgtga gcccgaagtc gtggtcggtc aacgtcgaga
      181 tccgcgacca cgtccacgcc ctcaaccacg gcacgacgat ggccaagggc ggcttcggcg
      241 gtatctcctg gtactcgatc cagcgctccg acggcaagct gacggcactg gagtcggccc
      301 gcggccgcca cgtgaacttc cgctggctcg gccacatcca ccagcgcgcc gagatccccg
      361 cgatggacgg cgagggcgag ctcttcgtcg tcggcagcct caagggcggc gacgagtacg
      421 ccctctacgg cctgcagtcc tactcaggcc cgcgccagca gctcgtcggc tgccacgaga
      481 agacgggcgt ctcctggcgc tacccgctct tcgtccagtt cggcgacgag cggccgagcc
      541 gttacgagga tctgctctca tgagcaccga cgcctacccc gagtggccct gcccctactg
      601 cgggcacgag gcgtggagcg agctgttcgc caagccgatg cggcgctgcg agcgcctcaa
      661 ccccgtcata tgcggtcact gcaacgtgct cttcaacgcc aactgggtcg acggctgctg
      721 gatgggccgc atgacggagg ccgagtggcc cggcagcttg gccgacttcg tggagatgaa
      781 ggccgaagcc ttacggttgg agttgtcgtg acctacacca tccaccacgg cgactgcctt
      841 gaagtcatgg ccgggatgcc cgatgcgtct gtggacttta tcttcaccga cccgccttac
      901 ggccacaaca acaacaacgg cgacctcatc caccgttggg aagccgccct cggtcggctt
      961 cccgacggtg cgaatccacc gttggctcgc cctatcgcca acgatggagc cgaggccgac
     1021 gaggtcttcg ctgctgccct gccgcacttc cgtcgcctgt tgcggacagg cggctgctgc
     1081 tgctgctgct gctgctgcgg cggcggcggc cccgacccga tgttcgcccg ctggtcactc
     1141 gccctcgacc gcgaactgga gttcaagcag atggtggtct gggacaaggg gccgatgggc
     1201 atgggctggc actaccggcg cagctacgaa tccgtgctcg tcgccatgaa gccgggcggg
     1261 ccgtgcaagt ggtatgacga cactgaccgc gtcgagaaca tcatccggcc gcactacaag
     1321 ggcatccgca agatcatccc gtccgccgac gagcacccga cgccgaagcc ggtcgggctg
     1381 gcggcgcact tcatcggcct gcacacgcag ccgggcgacg tggtactcga cccgttcatg
     1441 ggcgcgggct caaccggcgt tgccgcgcag aacctcggac gcgacttcat cggcattgag
     1501 attgaccagc actggtacgg cgtcgccgag cagcgcctcg ccacggccgc cgcgcaactg
     1561 acgctggcga caggctaggc ggcggcaatg aggctcatca cggtgcagga gcggtacgag
     1621 cgcgggcaga cgttccggct cgtgcacggc gactgccttg aggtcatggc cgggatggag
     1681 gccgggtcgg tggacgcggt ggtgacggac ccgccgtatg gcttggcgtt catgggcaag
     1741 cactgggacc acggcatccc cggcgtggac ttctggaccg aggcgctgga cgtgatgaag
     1801 ccgagcgcct acctcctcgc cttcggtggc acccgcac
//
LOCUS       DAOQYE010000465         1834 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_76131_length_1834_cov_2.967397, whole genome shotgun sequence.
ACCESSION   DAOQYE010000465 DAOQYE010000000
VERSION     DAOQYE010000465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76131_length_1834_cov_2.967397"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..691)
                     /locus_tag="PKN52_07885"
     CDS             complement(<1..691)
                     /locus_tag="PKN52_07885"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNQ99906.1"
                     /translation="MSPRASAVLLGAFLTIAMASTTTLQPAPATDLRWDQVVWYEVFV
                     RSFQDSNGDGVGDLRGLIDRLPYLSDLGVGGLWLMPTYPSPSYHGYDVTDYTAVNPDY
                     GTIDDMRALVSEAHALGIRVILDFVPNHTSRLHPWFLAALAGDERYRAYYRFESDPPP
                     IRGTRGGSAWHATPGGDYYLGLFGPGMPDLNLANPDVTEALYGAADFWLELGVDGFRV
                     DAIQHLIESEDG"
     gene            complement(688..>1834)
                     /locus_tag="PKN52_07890"
     CDS             complement(688..>1834)
                     /locus_tag="PKN52_07890"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNQ99907.1"
                     /translation="LVVLTALLLSLTVYAALRDTTPPQLYVEVPARLPVGAPLTLFAS
                     ADEPVTLTLEYAGGTVQVVEQETTFDTPAAAGANVAHLIAVDGAGNETTYDALVIGVP
                     ALEPTLSVAEGVVSGDPLGIWVRGLPGAAADPLLEATVTDVSLTVAGENVPLRRLVAE
                     DGTAEYRGVVATVMTVDPTTLDVDMSVVDEFGRAVTERRQVVLEPLPVTVEQLQIAPS
                     VLAVVTPEGQSLEARTIAAAWEHARPDPLWTEPFIAPIEGVTSSGFGDARRYAQGGPV
                     SFHYGLDLAAPQGTPIHATNDGVVVVAGPYPIKGGWTAIDHGDGVFSYYFHQSKILVA
                     VGDVVKRGDVIGEVGTTGLSTGPHLHWEMRVRQAASNPLAWVGKTFP"
BASE COUNT          264 a          601 c          701 g          268 t
ORIGIN      
        1 gaccgtcctc gctctcgatg aggtgctgga tggcgtccac gcggaagccg tcgacgccga
       61 gctccagcca gaagtcggcg gcgccgtaca gcgcctccgt cacgtcgggg ttggccaggt
      121 tcaggtcggg catgcccggg ccgaagagac ccaggtagta gtcgccgccc ggggtggcgt
      181 gccaggcgct gccgccgcgg gtgccgcgga tgggcggcgg gtccgactcg aaccggtagt
      241 aggcgcggta gcgctcgtcg ccggccagcg cggcgaggaa ccatgggtgc agcctcgacg
      301 tgtggttagg tacgaagtcg aggatcacgc gtatcccgag cgcgtgggct tccgagacga
      361 gcgcacgcat gtcgtcgatg gtcccgtagt cggggttgac cgccgtgtag tcggtgacgt
      421 cgtagccgtg gtagctgggg ctggggtagg tgggcatgag ccacagcccg ccgacgccga
      481 ggtcgctgag gtacggcaac cggtctatga ggccgcgcag gtcgccaacg ccgtcgccgt
      541 tcgagtcctg gaaggagcga acgaacacct cgtaccagac gacctggtcc cagcggaggt
      601 cggtggcggg cgccggctgc agcgtcgtgg tcgaggccat ggcgatggtg aggaacgcgc
      661 cgaggaggac ggcggacgcc cgcgggctca cgggaaggtc ttgccgaccc aggcgagcgg
      721 gttgctggca gcctgccgta cgcgcatctc ccagtggagg tgtgggccgg tcgagaggcc
      781 ggtggtgccc acctcgccga tgacgtcgcc gcgcttcacg acgtcgccga ccgccaccag
      841 gatcttcgac tggtggaagt agtagctgaa cacaccgtcg ccgtggtcga tcgcggtcca
      901 gccgcccttg atggggtacg gaccggccac caccacgacg ccgtcgttgg tggcgtggat
      961 gggtgtgccc tgcggcgcag cgaggtcgag accgtagtgg aaggacacgg ggccgccttg
     1021 cgcgtaacgt cgggcgtcac cgaagccgct ggatgtcacg ccctcgatcg gggcgatgaa
     1081 gggctccgtc cacagcgggt cgggtctggc gtgctcccag gcggcggcga tcgtccgcgc
     1141 ctccaggctc tggccttccg gcgtgacgac cgccaagacg gacggggcta tctggagttg
     1201 ctccacggtg acaggcagcg gttcgagcac gacttggcgc ctctccgtga ccgcgcgccc
     1261 gaactcgtcc acgacgctca tgtccacgtc cagcgtcgtc gggtcgacgg tcatcaccgt
     1321 ggccaccacg ccgcggtact cggccgtgcc gtcctccgcc acgagccgtc gcaggggcac
     1381 gttctcgccc gccacggtga ggctgacgtc cgtgacggtc gcctcgagga gcgggtcggc
     1441 ggcggcgccg ggcagcccgc ggacccagat gccgagaggg tcgcccgaca cgaccccttc
     1501 cgcgacggag agcgtcggct cgagcgccgg gaccccgatc acgagggcgt cgtacgtcgt
     1561 ctcgttcccg gcgccgtcca cggcgatcag gtgggcgacg ttggctccgg cggcggcggg
     1621 cgtgtcgaag gtcgtctcct gctccactac ctggacggtg ccgcccgcgt actcgagcgt
     1681 gagcgtgacg ggctcgtcgg cgctggcgaa gagggtcagc ggcgccccga cgggcaggcg
     1741 cgccggcacc tcgacgtaga gttgcggcgg cgtggtgtcg cgcagcgcgg cgtagacggt
     1801 gagactgagc aacaacgcgg tgaggaccac gagc
//
LOCUS       DAOQYE010000466         1831 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_76348_length_1831_cov_2.583896, whole genome shotgun sequence.
ACCESSION   DAOQYE010000466 DAOQYE010000000
VERSION     DAOQYE010000466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76348_length_1831_cov_2.583896"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /locus_tag="PKN52_07895"
     CDS             <1..368
                     /locus_tag="PKN52_07895"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNQ99908.1"
                     /translation="GRNLMAGWMGRVGFHLELGGFDEGANDLRVQGIVEAAQRYFPRL
                     TAAELAEPPRWHGFRPLSPDGLPYLGRSRRVPNLTVASGHGMMGLSLAPVTGKLVGET
                     VAGEEPSLPIQVLAVERFS"
     gene            498..743
                     /locus_tag="PKN52_07900"
     CDS             498..743
                     /locus_tag="PKN52_07900"
                     /inference="COORDINATES: protein motif:HMM:NF015947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HNQ99909.1"
                     /translation="MALVRTATLSSKGQVTIPLAVRKALKLKQGDVVRFVVDGDSATV
                     ELVASEDPFSAWAGAWREGQGKTIEEIVQDERELRGW"
     gene            737..1156
                     /locus_tag="PKN52_07905"
     CDS             737..1156
                     /locus_tag="PKN52_07905"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNQ99910.1"
                     /translation="MVTAVDTNVLSALLIGGPEESAAARDVLTAAARSGELVISPIVY
                     AELRAAPRGTEAEVGVFLSSLRVEVVWTVGEGVWRDAAAAFSAYAARRRVRHDGMPKR
                     FLADFLVGAHACWLKCRLLTFDQGKYRAAFPELELVP"
     gene            complement(1360..1737)
                     /locus_tag="PKN52_07910"
     CDS             complement(1360..1737)
                     /locus_tag="PKN52_07910"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNQ99911.1"
                     /translation="MSVLDASAVLALLNGEPGARAVSAHIANGAMISAVNYAEVATKL
                     ADLALPDAAARGILEALGLLVQPFDDGQALTTGVMRSGTRDAGLSLGDRACLALAHHV
                     GMTAVTADRAWQGLTDVVVEVIR"
BASE COUNT          311 a          563 c          623 g          334 t
ORIGIN      
        1 tcggccgcaa cctgatggcc gggtggatgg gccgggtcgg cttccatctg gagcttggcg
       61 gcttcgacga gggcgccaac gacctgcgtg tccaagggat cgtcgaggcc gcgcagcgct
      121 atttcccgcg cttgaccgcc gccgagttgg ccgaaccgcc gcgctggcac ggcttccgtc
      181 ccctgtcacc tgatgggttg ccgtacctgg gccgctcgcg gcgcgtgccc aacctgaccg
      241 tggcgtcggg ccacgggatg atgggcttga gcctggcgcc cgtaacgggg aagctcgtcg
      301 gggagaccgt ggcgggtgag gagccttcgc tgcctatcca agtgttggcg gtggaacgtt
      361 tttcgtgacg tcgaagggtc cagcgcccac accggctaat gctcgtctgc gggctggaac
      421 gccgtctagg taggaatcca tcgttgttgc accctgtaga cggctaccat atgctgtcct
      481 tactgatgga ggtaaggatg gcattagtaa ggacagctac gctgagcagt aaaggacagg
      541 tgaccatccc actcgcggta cgcaaagcct tgaaactcaa gcaaggcgac gttgtgcgct
      601 tcgttgtcga cggcgatagc gcgaccgtcg agctggttgc ctccgaggat ccattctccg
      661 cctgggcggg cgcttggcga gaaggccagg gcaagaccat cgaagagatc gtgcaggacg
      721 agcgcgagtt gcgtggatgg taaccgcggt agacacgaac gtactttccg ccctcctcat
      781 cggcggcccg gaggagagtg ctgccgcgcg agacgttctc acggccgcgg cacggtcggg
      841 cgagttggtg atctctccga tcgtctacgc ggaattgcgg gccgctccga ggggaacgga
      901 ggctgaagtc ggggtcttcc tctcctcctt gagggtagag gtcgtgtgga cggtgggtga
      961 aggtgtctgg cgtgatgcgg cggctgcttt cagcgcctat gcagcgcgca gacgcgtacg
     1021 ccatgacggt atgcccaagc gcttcctggc ggacttcctg gtaggagcgc acgcgtgttg
     1081 gttgaagtgc cgcctgctca ccttcgacca gggcaagtac cgagcggcat tccctgaact
     1141 cgagttggtt ccctgacggc gccgagcggc tggtcgcagg aaccggagtc gccttgtggg
     1201 gcattgcgca tctggcgacc gaggcgaagg gctgtcgttc atttacgacc ggtccgaata
     1261 gggctgaagc gccaagtggt tgcacctgcc tagcccaaga ccgccccgcc agatcgcttc
     1321 cgcagacccg aacgacggga aatgtcgccc ctccagcgcc tagcggatca cttcgacgac
     1381 gacatccgtg aggccttgcc aggctcggtc ggcggtcacg gccgtcatgc cgacgtgatg
     1441 ggcaagagcc aggcaggcac ggtcccccaa cgaaaggccg gcatcacgag tcccgctgcg
     1501 catgacgcca gtcgtcaggg cctgtccatc atcgaagggc tgcacgagta gaccaagcgc
     1561 ctcaaggatc cctcgcgcgg ccgcgtcggg aagagcgagg tcggccagct tcgtagcgac
     1621 ctcggcgtag ttcactgcgc tgatcatcgc gccattcgcg atatgcgctg atacagcgcg
     1681 cgctccgggc tcgccgttga ggagcgcgag gacggcggac gcatccagta cgctcacgac
     1741 tcttcccgtg ccgcctctcg gcgttcacga aggaagtcct ccaccactgg cgtgccttct
     1801 ggaacgtacg tgcggaacag gtcctgggct t
//
LOCUS       DAOQYE010000467         1831 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_76370_length_1831_cov_2.163851, whole genome shotgun sequence.
ACCESSION   DAOQYE010000467 DAOQYE010000000
VERSION     DAOQYE010000467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76370_length_1831_cov_2.163851"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="PKN52_07915"
     CDS             <1..516
                     /locus_tag="PKN52_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99912.1"
                     /translation="ALFGTDAFDALPGGVLSVSDVLVIEQPAPAPDAAADQPVSADED
                     LAGDGADAAPAQATIDTYVVLIADRVAPRVRPLDDVYAQVEQAVLAQKRQAARTAWLS
                     DQRAAAEIVETSVPDLVLPTDTVQGSDATATPEGVGGEAPAEETPATDEAPATDEAPA
                     GDEAPAPDANN"
     gene            589..>1831
                     /gene="purH"
                     /locus_tag="PKN52_07920"
     CDS             589..>1831
                     /gene="purH"
                     /locus_tag="PKN52_07920"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HNQ99913.1"
                     /translation="MSTQSREGRSRIECGRIERGRAECGRALISVYDKRGLEEFARGL
                     VELGFELVSTGRTLAALTGWGIPAVAVSDVTGFPEILDGRVKTLHPKVHGGILARREP
                     AHLSELAAHDIVPVEVVVSNLYPFRETVADPGVSEAQAMEQVDIGGPAMLRAAAKNHP
                     GVVVVVRPEDYGSTLAALRRGITAEERRELAREAFAHTAAYDAAIVEYLSRGEELPRH
                     LDVALERAEILRYGENPHQQGARYREVGKRSLWDAAEQHSGLALSYLNLFDAEAAWRL
                     AHELVGAAALQGSPGPNRAACVIVKHANPCGAAWGADLADAYTKAFDADPKSAFGGVV
                     ALPGHVDLALAELIVARPKADVLMASGFAPEALELLARKRKNTRVLTLPPPGPAGLQL
                     RRLDGGFLVQHADAVSLSRDGW"
BASE COUNT          276 a          678 c          630 g          247 t
ORIGIN      
        1 gcgctcttcg ggacggacgc gttcgacgcg ctgcctggcg gcgtcctctc ggtgagcgac
       61 gtcctcgtga tcgagcagcc cgccccggct cccgatgcgg cggccgacca gcccgtttcc
      121 gccgacgagg acttggccgg cgacggcgcc gacgccgccc cggctcaggc caccatcgac
      181 acctacgtcg tgctcatcgc cgaccgcgtc gcgccgcgcg tgaggccgct ggacgacgtc
      241 tacgcccagg tcgagcaggc ggtcttggct cagaagcgcc aggcggcccg cacggcgtgg
      301 ctctcagacc agcgcgccgc ggccgagatc gtagagacct cggtgccgga cctcgtgctg
      361 cccaccgata ccgtccaggg ctcagacgcc acggccacac ccgagggggt cggcggcgag
      421 gcgccagccg aagagactcc ggccaccgac gaggcccccg ctaccgacga ggctcccgcc
      481 ggcgatgagg ctccggcgcc cgacgccaac aactgaacct agtcgcacca cctggcgcgg
      541 gcggctggag gtcatggccg cccgcgcccg ttctggaagg gagccagggt gagcacccag
      601 tcgagagaag gacgcagccg catagaatgc gggcgcatag aacgcgggcg cgcagaatgc
      661 gggcgcgcgc tcatcagcgt ctacgacaag cgcggccttg aagagttcgc ccgcggcctc
      721 gtcgagctcg gtttcgagct cgtcagcacc gggcgcacac tcgccgcgct cacaggatgg
      781 ggcataccgg cggtggccgt cagcgacgtc accggcttcc ccgagatcct tgacggtcgc
      841 gtgaagacgc tgcacccgaa ggtccatggg ggcatcctcg cgcgccgcga accggcccac
      901 ctgagtgagc tcgccgctca cgacatcgtc ccggtggaag tagtcgtctc caacctctat
      961 ccgttccggg agactgtcgc cgaccccggc gtgagcgagg cgcaggccat ggaacaggtc
     1021 gacatcggcg gtcccgccat gctccgcgcc gccgccaaga accatccggg tgtcgtcgtg
     1081 gtcgtaaggc ccgaggatta cggctccacc ctggcggcgt tgcggagggg catcacggca
     1141 gaggagcgcc gcgagcttgc gcgcgaggcc ttcgctcaca ccgccgccta cgacgccgcc
     1201 atcgtcgagt acctgagcag gggcgaggag ttgccgcgcc atctggacgt cgcgcttgaa
     1261 cgtgccgaga tcttgcgcta cggcgagaac ccccaccagc agggtgcgcg ctaccgggag
     1321 gtcggcaagc gctcgctctg ggacgccgcc gagcagcact ccgggctcgc gctctcctac
     1381 ctgaacctct tcgacgcgga ggccgcctgg cgcctagcgc acgagctcgt cggcgccgcg
     1441 gcgctgcagg gctcaccagg tccgaaccgg gccgcctgcg tgatcgtcaa gcacgccaac
     1501 ccgtgcggcg cggcttgggg cgctgacctg gcggacgcct acacgaaggc gttcgacgcc
     1561 gacccgaagt ccgccttcgg cggggtcgtg gccttgcctg gtcacgtcga cctggccctg
     1621 gccgagctca tcgtggcgcg gcctaaggcc gacgtgctca tggcctccgg cttcgcgccc
     1681 gaggccctcg agctcctggc ccggaagcgc aagaacacac gcgtgctcac gctgccgcca
     1741 cctggccccg ccggtctgca gttgcggagg ctcgacggcg gcttcctcgt gcaacacgcc
     1801 gatgccgtga gcctgagccg cgacggttgg c
//
LOCUS       DAOQYE010000468         1830 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_76414_length_1830_cov_2.709859, whole genome shotgun sequence.
ACCESSION   DAOQYE010000468 DAOQYE010000000
VERSION     DAOQYE010000468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76414_length_1830_cov_2.709859"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(312..1352)
                     /locus_tag="PKN52_07925"
     CDS             complement(312..1352)
                     /locus_tag="PKN52_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNQ99914.1"
                     /translation="MVQALAALLLLLAPVGLAQNLIPINFQSKWFPQAQFAGYFVAQD
                     KGFYAEEGLDVTVLDGGNVNPTVAVASGNADFGTDWVANILTQRDQGLEVVNIAQIYQ
                     TSGYRLVALKSSGIETIADMAGRKVGVWAFGNEFAAEAVFSAAGLTSNLDPTVTNPDV
                     DAVVYAFDPSLVFPNEVDVASAMTYNELDQIIGLGYDLDTLSILDPASIGADLLEDSL
                     FATPATLASTNFKGSGLTGAEVAQRFLRASIRGWEYAVANQDEAVQIVLSHCGDTCAG
                     SGSRQSPLIHQTWQMAEVAKLVKPTADTQVGALDRAAFERSVNLLVEVGLINQAYTWE
                     QAVDPSIYDAIH"
     gene            complement(1434..>1830)
                     /locus_tag="PKN52_07930"
     CDS             complement(1434..>1830)
                     /locus_tag="PKN52_07930"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNQ99915.1"
                     /translation="VVIVTFFPVVLNTVQGLTLVDPLKLELLRSYGAGPLAVFFKLRV
                     PNALPYLFNALKVAVVVGVVSVIVAEFLIPGPPNGLGQRISLSAHRGAFDIVFAAIVV
                     SSLISMALYWAVGLLERAFTSWHPSTRGS"
BASE COUNT          322 a          541 c          688 g          279 t
ORIGIN      
        1 cgccgagttg acgccggcct accgagccct ggagcgaagg gcacctgcgt agaaacgtga
       61 gttcaaggta gctgccaggc acagtgggtc ggaacgccgc gcgccaggcg ccgaacaatc
      121 gaagtcgagg tgggcgtggc ggcggagggt gcccgaagta ctgccatagc gcagcgtagg
      181 cagtaggtag cggcaccctc cgccgccacg cccaccagac cacgcgacga agccacgaag
      241 ccaacgagaa gcgcgccgac cccgcgaagg gccggcgcgc ctgagagcag ctacgtcagc
      301 gagctagcag ctcagtggat ggcgtcgtag atgctcgggt cgacggcctg ttcccacgtg
      361 taggcctggt tgatgaggcc gacctcgacg aggaggttga cggaacgctc gaacgcggcg
      421 cggtcgaggg cgccgacttg cgtgtcggcg gtcggcttga cgagcttggc gacctcggcc
      481 atctgccagg tctggtggat gagtgggctc tggcggctgc cggagccggc gcacgtgtcg
      541 ccgcagtgcg agaggacgat ctggacggcc tcgtcctggt tcgcgacggc gtactcccag
      601 ccgcggatgg aggcgcgcag gaagcgctgc gccacctcgg cgccggtcag cccggagccc
      661 ttgaagttcg tggaggccag cgtggcgggg gtggcgaaga ggctgtcctc gaggaggtcg
      721 gcgccgatgc tagccgggtc gaggatcgag agcgtgtcga ggtcgtagcc gaggccgatg
      781 atctggtcga gctcgttgta ggtcatggcg ctcgccacgt ccacctcgtt cgggaagacg
      841 agcgaggggt cgaaggcgta gacgaccgcg tcgacgtcgg ggttggtgac ggtcgggtcg
      901 aggttcgagg tcaggccggc ggcggagaac acggcctcgg ccgcgaactc gttgccgaac
      961 gcccacacgc cgaccttgcg gccggccatg tcggcgatcg tctcgatgcc ggagctcttg
     1021 agcgccacga ggcggtagcc ggaggtctgg tagatctggg cgatgttgac gacctcgagg
     1081 ccctggtcgc gctgcgtgag gatgttggcg acccagtcgg tgccgaagtc ggcgttgccg
     1141 gaggcgacgg cgacggtcgg gttcacgttg ccgccgtcga gcaccgtcac gtcgaggcct
     1201 tcttccgcgt agaagccctt gtcctgcgcc acgaagtagc ccgcgaactg cgcctgcggg
     1261 aaccacttgg actggaagtt gatggggatc aggttctggg cgagccccac cggcgcgagc
     1321 aagagtagga gagcggctag tgcttggacc atccgtttca ttcggtacct cccaatggct
     1381 ttgctacgag gttacagcgt gttggacgtc gagggccttg agcgttcaca tactcacgac
     1441 cccctcgtcg atgggtgcca agaggtgaag gcccgctcga gcaggccaac ggcccaatag
     1501 agggccatgg agatgaggct cgacacgacg atggcggcga acacgatgtc gaacgccccg
     1561 cggtgcgccg agaggctgat gcgctgcccg aggccgttgg gcgggcccgg gatgaggaac
     1621 tccgcgacga tgacgctgac gacgccgacc acgaccgcga ccttcagcgc gttgaagagg
     1681 tagggcaggg cgttcgggac gcgcagtttg aagaagaccg cgaggggacc ggcgccgtac
     1741 gaacgtagga gttccagttt cagggggtcg acgagcgtga gcccctgcac cgtgttgagg
     1801 acgaccggga agaaggtgac tatcacgacc
//
LOCUS       DAOQYE010000469         1827 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_76617_length_1827_cov_2.876975, whole genome shotgun sequence.
ACCESSION   DAOQYE010000469 DAOQYE010000000
VERSION     DAOQYE010000469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76617_length_1827_cov_2.876975"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..856
                     /locus_tag="PKN52_07935"
     CDS             <1..856
                     /locus_tag="PKN52_07935"
                     /inference="COORDINATES: protein motif:HMM:NF023242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase C39 family protein"
                     /protein_id="HNQ99916.1"
                     /translation="LLAAAEEGARERGAIVMRLEVREDNAAAIALYSASGYTVSGRTA
                     DYYEDHSTALRMRKRFAKGGATVLGVPYYPQTLDFTCGPSSLMMAMRYHGYPVPLERW
                     LELSLWREATTVFLLSGHGGCSAHGIAVAALRRGFQASVVTSDPGTPFEATVQGEKRE
                     VVRIAHTTFERELRGFGGKVELRDFGHQDVADIIERGAVPIVLVSGHRLYAARVPHWV
                     VVTGFDDDHIYLHDPIVPEGSDRADSVHLPIKRELFDKITRWGRGRHRSMVVVERWGN
                     IRRLLQGN"
     gene            complement(831..1706)
                     /locus_tag="PKN52_07940"
     CDS             complement(831..1706)
                     /locus_tag="PKN52_07940"
                     /inference="COORDINATES: protein motif:HMM:NF020032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99917.1"
                     /translation="MARLAIFTERYTIRSSVELTALTNFRLAAFELGHDLDFLFKNEL
                     KYLRNYDGIFIRALTDPLNTTYVVARQAEILGKRVLDHSSAILICCDKVNMYSRLAAR
                     NVPIPETRFLVESDLNALSASRLFEELGTPLVLKAPNSSFSAYVDKVATPDAFVKVGK
                     RFLRRADRIVVQQYMPSEFDWRVITLNGCVLAVVKYAFAPAQWRLMDRTDGGEWAKTK
                     AIHRDAANPKLTATALAAANAIGTSLYGVDIKEIDGEFFVIEVNDNPTIAAGDEDQEN
                     PEIYAEIISYLAGDV"
BASE COUNT          286 a          583 c          641 g          317 t
ORIGIN      
        1 gctgttggcc gccgccgagg agggcgcgcg cgagcgcggc gccatcgtca tgcggctgga
       61 ggtgcgcgag gacaacgctg ccgccatcgc cctgtactcc gcttctggct acacggtgag
      121 cgggcgcacc gccgactatt acgaggacca ctcgaccgcc ctgcgcatgc gcaagcgctt
      181 cgccaagggc ggggccaccg tgctcggcgt cccttactac ccgcagacgc tcgacttcac
      241 ctgcgggcct tcttcgctca tgatggccat gcgctatcac ggttaccccg tgccgctcga
      301 gcgctggctc gagttgagcc tgtggcgcga ggccaccacg gtcttcctgc tatcgggcca
      361 cggaggttgc agcgcccacg gcatcgccgt ggccgccctg aggcggggct tccaggcgag
      421 cgtggtgacg tccgaccccg gtaccccctt cgaggcgacg gtgcagggcg agaagcggga
      481 ggtcgtccgc atcgcgcata cgaccttcga gcgcgagctg cgcggcttcg gcggcaaggt
      541 cgagctgcgc gacttcggtc accaggacgt ggccgacatc atcgagcgcg gcgccgttcc
      601 catcgtgctg gtgtcgggtc atcgcctcta cgcggcgcgc gtgccgcact gggtcgtcgt
      661 gacgggcttc gacgacgacc atatctacct gcacgacccc atcgtccccg aggggagcga
      721 cagggccgac agcgtccacc tacccatcaa gcgggagctc ttcgacaaga tcacgaggtg
      781 ggggaggggt cgccaccgct ccatggtcgt cgtggagcgg tggggcaaca tcagacgtct
      841 cctgcaaggt aactgatgat ctccgcgtag atctccgggt tctcctggtc ctcgtcgccg
      901 gcggcgatgg tggggttgtc gttgacctcg atgacgaaga actcgccgtc gatctccttg
      961 atgtcgacgc cgtagaggct cgtgccgatg gcgttggccg cggcaagcgc ggtggccgtg
     1021 agcttggggt tggcggcgtc ccggtggatg gccttcgtct tcgcccactc gccgccgtcg
     1081 gtacggtcca tcagccgcca ctgggccggc gcgaacgcgt acttgacgac cgcaaggacg
     1141 cagccgttca acgtgatcac gcgccagtcg aactcgctcg gcatgtactg ctggaccacg
     1201 atgcgatcgg cgcggcggag gaagcgcttg ccgaccttga cgaaggcgtc cggcgtggcc
     1261 accttgtcga cgtaggcgct gaagctcgag ttcggcgcct tgagcacgag cggggtgccg
     1321 agctcctcga agagccgcga ggccgacaag gcgttgaggt cgctttcgac caggaaacgc
     1381 gtctcgggga tgggcacgtt gcgcgcggcc agccgcgagt acatgttgac cttgtcgcag
     1441 cagatgagga tggcagacga gtggtcgagg acgcgcttgc cgaggatctc ggcctggcgc
     1501 gccaccacgt aggtcgtgtt cagggggtcc gtgagggcgc ggatgaagat accgtcgtag
     1561 ttccgcaggt acttcagctc gttcttgaac aggaagtcga ggtcgtggcc gagctcgaac
     1621 gccgccaggc ggaagttcgt cagggccgtg agctccaccg aggaccggat cgtgtagcgc
     1681 tcggtgaaga tagctaggcg cgccatgtgc cgaggccctc caggaccgcc ctttcgttct
     1741 cgtcgaggtc cttgaacggc aagggttcga tggccgagag ctggtaggcc ttgagggtga
     1801 cgatgaccct cacgcgcatc aagggca
//
LOCUS       DAOQYE010000470         1820 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_77120_length_1820_cov_2.908782, whole genome shotgun sequence.
ACCESSION   DAOQYE010000470 DAOQYE010000000
VERSION     DAOQYE010000470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77120_length_1820_cov_2.908782"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="PKN52_07945"
     CDS             <1..255
                     /locus_tag="PKN52_07945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99918.1"
                     /translation="IAMLAVLLTVAFMNAAGGALATLSVLIVMQLFVVFPGLEPFLLT
                     TQLQTYSSPTADLTWAVALIALYSALFAAVAVVLFERKDF"
     gene            255..1607
                     /locus_tag="PKN52_07950"
     CDS             255..1607
                     /locus_tag="PKN52_07950"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNQ99919.1"
                     /translation="MRVRHAWRLVQRPAHLVAALIVAAALVGNASAQTIAYTVQVLAL
                     SDRDAALGVQTDLVRQGYPAYVVRSTSAQGDVFRVRVGAFANRSAAVNYAEAMPTVGG
                     GRPVPALAESIPGGIVPLAPRLLLEEEVSGMEARLLAFPDGALVLRLQWRVPLTQAEY
                     VVIQGGDVERLRAWRLAEGPDGARLRVRELQLWPDTWQQDSQEVRQAFATNLVALVAE
                     RLGVRTSEVDAARYQPRADEAPRLFVVERVAAGGDAPELLGVGLPASGMAAAGPIAYL
                     GVDPSRLPSLPAGARVDLATGSVMGELEVVPWPLSGTDGDQGDAGAPTGAPVGAPAAD
                     TPDATPGAAEDAAEDAAEDAPEDAAEDTTSAEESAAGLGETPSGAESDSRAVTGTVAG
                     DGWVASPDGEFIRLTTVDALTGATVSWRAGVGMPIWSDGHHLLALRSGKLLVYDFLPR
                     "
BASE COUNT          214 a          664 c          662 g          280 t
ORIGIN      
        1 atcgcgatgc tcgcggtgct tctgaccgtc gccttcatga acgcggcggg cggcgcgctc
       61 gccaccctgt ccgtgctcat cgtcatgcag ctcttcgtcg tcttcccggg gctcgagccg
      121 ttcctcctga ccacgcagct gcagacgtac tccagcccga ccgccgacct cacgtgggcc
      181 gtagcgctca tcgccctcta ctccgcgctc ttcgccgcgg ttgccgtggt cctgttcgag
      241 cggaaggact tctagtgcgc gtgcgccacg cttggcgcct cgtgcagcgc cccgcccacc
      301 tcgtcgccgc cctcatcgtg gcggccgcgc tagtgggcaa cgcctcggcc cagaccatcg
      361 cttacacggt ccaggtcctc gctctgtcgg accgcgacgc ggcgctcggg gtgcagaccg
      421 acctcgtgcg tcagggctat ccggcgtacg tcgtgcgctc cacctcggcg cagggcgatg
      481 tcttccgcgt gcgcgtcgga gcgttcgcca accggtccgc cgccgtgaac tacgcggagg
      541 ccatgccgac ggtaggcggc gggcggccgg tgccggccct ggccgagtcg atccctggcg
      601 gcatagtccc gctggcgcct cggctgttgt tggaggaaga ggtcagcggc atggaggcgc
      661 ggctgctggc gttccctgac ggggcgttgg tgctccggtt gcagtggcgg gtcccgctca
      721 cgcaggccga gtacgtcgtg atccagggcg gtgacgtcga gcgcctccgc gcctggcggt
      781 tggccgaggg tccggacggc gcgcggttgc gggtgcgcga gctgcagctc tggccggaca
      841 cttggcagca ggacagccag gaggtgcgcc aggccttcgc caccaacctc gtggcgttgg
      901 tcgccgagcg acttggggtg cggacctcgg aggtggacgc cgcgcgctac caaccgcggg
      961 ccgacgaggc gccgcggctc ttcgtcgtgg aacgggtggc cgcaggcggt gacgctcccg
     1021 agctccttgg cgtcgggctc ccggccagcg gcatggcggc ggccgggccg atcgcctacc
     1081 ttggcgtcga cccgagccgg cttccgagcc tgccggcggg cgcgcgcgtc gaccttgcga
     1141 ccgggagcgt gatgggcgag ctcgaggtcg tcccctggcc cttgagcggg acggacggcg
     1201 accagggcga cgcgggcgcg ccaactggcg cgccagttgg cgcgcccgcc gccgacaccc
     1261 ccgatgccac gcccggcgcc gcggaggacg ctgcagaaga tgccgcggaa gatgccccag
     1321 aagatgccgc ggaagacacc acctccgcgg aggagtccgc cgccggcctg ggtgagaccc
     1381 cgtctggggc ggagtcggac tccagagcgg tcaccggaac ggtggcgggc gacggctggg
     1441 tggcttctcc ggacggcgag ttcatccgcc tcaccaccgt agacgccttg acgggagcga
     1501 ccgtgagctg gcgggccggc gtaggcatgc ccatctggtc ggacgggcac cacctactgg
     1561 ctctccgctc gggcaagctg ctcgtctacg acttcctgcc gcgctgaccg cttgcgtctc
     1621 ggccaccgcc ctggctcgtg gcgttgtcag gcgccaggca ggctagcggc ctgccaagcg
     1681 cgcgttggcc gcccacctgg gccctcctca agcgggcgcc aggccggccg cggctgccgc
     1741 cttcgcgacc ggttcgatgg cgtgttccac cagccacttc tcgaccgcca tggcggcccg
     1801 cgtgccggcg ccgaccgagg
//
LOCUS       DAOQYE010000471         1816 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_77420_length_1816_cov_3.124361, whole genome shotgun sequence.
ACCESSION   DAOQYE010000471 DAOQYE010000000
VERSION     DAOQYE010000471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77420_length_1816_cov_3.124361"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1184
                     /locus_tag="PKN52_07955"
     CDS             <1..1184
                     /locus_tag="PKN52_07955"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNQ99920.1"
                     /translation="SGGAEAVKALWGTDVDVSTTVWSNAFVPATFQPGERIILQRNPY
                     FGEWNVDSAGNALPYLDTYSMTIAGDLDSALNLYLAGDVDQFNPRNLDDIGVINNAVN
                     NGDIDVLVMESVSPVASSQFIVWNWNKASDPDKEALFRNANFRRAMAYVMDRDAIIDL
                     VYNGAGIPMYTNVYPINDYWVNHDAEQYPYNPEKAAELMASIGYDRKNADGILVNAEG
                     KTASFILATNAGNTAREQIAAIFADSAREIGVDVQVQAIDFNLLVDQLLSEGDDRPFD
                     AILIGLTGGSRDWPFGVNVVPCGTNLHSYNTSGECLTAQETLMSQLFNVGRETLDTEA
                     ARDIGYEIQAVEADLAPYLYAVSPMAHYSWSSSLHGYHNEGQINPLLGAYELPLVFMA
                     R"
     gene            1351..>1816
                     /locus_tag="PKN52_07960"
     CDS             1351..>1816
                     /locus_tag="PKN52_07960"
                     /inference="COORDINATES: protein motif:HMM:NF040248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ99921.1"
                     /translation="MFVYILRRLFQLIPTFIGATMLMFLISQLVPGDFFKAKELEPNV
                     RPETIQRMRAEFGLDKPVYVQYVNWMKNLVTGNLGQSFVSNQPVWQRIARPMRNSMYL
                     AILSIILLWLVSIPAGVYSAVRQYSFGDQAVSVVSYLGLATPNFFLAQVIILA"
BASE COUNT          335 a          676 c          501 g          304 t
ORIGIN      
        1 gctccggcgg cgccgaggcc gtcaaggccc tctggggcac cgacgtcgac gtctcgacca
       61 ccgtctggtc gaacgccttc gtccccgcca cgttccagcc gggcgagcgc atcatcctcc
      121 agcgcaaccc gtacttcggc gagtggaacg tcgactccgc cggcaacgcg ctgccctacc
      181 tcgacaccta cagcatgacg atcgccggcg acctcgattc cgccctgaac ctctacctgg
      241 ccggcgacgt cgaccagttc aacccgcgta acctcgacga catcggtgtc atcaacaacg
      301 ccgtcaacaa cggcgacatc gacgtcctcg tgatggagtc ggtcagcccg gtcgcgagca
      361 gccagttcat cgtctggaac tggaacaagg cctccgaccc cgacaaggaa gccctcttcc
      421 gcaacgcgaa cttccgccgc gccatggcgt acgtcatgga ccgcgacgcg atcatcgacc
      481 tcgtctacaa cggcgccggc atcccgatgt acacgaacgt ctacccgatc aacgactact
      541 gggtgaacca cgacgccgag cagtacccgt acaaccccga gaaggccgcc gagctcatgg
      601 cctccatcgg ctacgaccgc aagaacgccg acggcatcct cgtgaacgcc gaaggcaaga
      661 cggcctcctt catcctcgcc acgaacgccg gcaacacggc gcgcgagcag atcgcggcca
      721 tcttcgccga ctccgcccgc gagatcggcg tggacgtcca ggtgcaggcc atcgacttca
      781 acctcctcgt cgaccagctc ctctccgagg gcgacgaccg tccgttcgac gccatcctca
      841 tcggcctcac cggcggctcg cgcgactggc cgttcggcgt gaacgtcgtg ccttgcggca
      901 ccaacctgca cagctacaac acgagcggtg agtgcctcac ggcgcaggag acgctcatga
      961 gccagctctt caacgtcggc cgcgagacgc tcgacaccga agccgctcgc gacatcggct
     1021 acgagatcca ggccgtcgaa gccgacctcg cgccgtacct gtacgccgtc agcccgatgg
     1081 cgcactacag ctggtcctcc agcctccacg gctaccacaa cgagggccag atcaacccgc
     1141 tcctcggcgc ctacgagctc ccactcgtct tcatggcccg gtagtacggg gcaaagggac
     1201 cttcaggtct ttcggagtgg gcggctacac gccgcccact cccaactacg atctatcttg
     1261 agacttatgt tcaacctcaa cgtcgccgag acggtccggg ccggtacggc cgctcctggt
     1321 tcgcgcagcg tcggccggga ggcgtagccg gtgttcgtct atatcctcag gcgcctgttc
     1381 caactgatcc cgacgttcat cggggccacg atgctgatgt tcctcatcag ccagctcgtg
     1441 ccgggcgact tcttcaaggc caaggaactc gagccgaacg tccgccccga gaccatccag
     1501 cgcatgcgcg cggagttcgg gttggacaag cccgtctacg tccagtacgt caactggatg
     1561 aagaacctcg tgacgggcaa cctcggccag tcgttcgtct ccaaccagcc ggtctggcag
     1621 cgtatcgcgc ggcccatgcg caacagcatg tacctcgcca tcctctccat catcctgctc
     1681 tggctcgtgt cgataccggc gggcgtctat tcggcggtga ggcaatactc cttcggagac
     1741 caggccgtga gcgtggtctc ctatctcggc ctcgccacgc cgaacttctt ccttgcccag
     1801 gtgatcatcc tcgccc
//
LOCUS       DAOQYE010000472         1810 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_77849_length_1810_cov_2.494017, whole genome shotgun sequence.
ACCESSION   DAOQYE010000472 DAOQYE010000000
VERSION     DAOQYE010000472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77849_length_1810_cov_2.494017"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..818
                     /locus_tag="PKN52_07965"
     CDS             <1..818
                     /locus_tag="PKN52_07965"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNQ99922.1"
                     /translation="LASAAADGFVAPVDVDAAMNAAKGMPVLYVAEEGGGATGFLYVF
                     APRRDEIEVNALVAPERRRKGVFTALLREAVAHSAPFGYGKGLLVCDARSASGRAVLE
                     RWSVPFEHAEHQMALTLTARPGPVREGLDLSEARLADLPELAALSAAAFGMDLQSTAE
                     VMGMSIRSGERTQWVLRDGGKPVGLCAKAEKDDAYLLYGLAIHPSERRRGYARAMLDL
                     VAGRALDAGVRSVVLDVDSANEAAIALYEGYGFEQTQSALYHAFEFARFGGAQ"
     gene            complement(774..1166)
                     /locus_tag="PKN52_07970"
     CDS             complement(774..1166)
                     /locus_tag="PKN52_07970"
                     /inference="COORDINATES: protein motif:HMM:NF017355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HNQ99923.1"
                     /translation="MTRLDPYKGYTAELEIDVDAGVLDGVVIGLRDVIHFQGSTVREA
                     EQAFHDSVDEYLEWCRELGEAPEKPYSGKVVIRMEPGLHRRLAQRSEQDGISINSLAI
                     GAIEQLLNQAKDAESLRAPEPGELERVI"
     gene            1606..>1810
                     /locus_tag="PKN52_07975"
     CDS             1606..>1810
                     /locus_tag="PKN52_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HNQ99924.1"
                     /translation="MFAVDLRSDTVTVPTSEMRAAMAAAEVGDDVYAEDPTVNRLQET
                     AAELTGFEGALFVPSGTMSNQIAL"
BASE COUNT          274 a          607 c          603 g          326 t
ORIGIN      
        1 cgctcgccag cgccgccgcg gacggcttcg ttgcgcccgt agacgtcgac gccgccatga
       61 acgcggcgaa gggcatgccc gtcctgtacg tcgccgagga aggcggaggc gccaccggct
      121 tcctgtacgt cttcgccccg cggcgcgacg agatagaggt caacgccctc gtggctcccg
      181 agcggcgccg gaagggcgtg ttcacggcgc tcctccgcga ggcggtggcc catagcgctc
      241 cgttcggcta cggcaagggg ttgctcgtct gcgacgcgcg ctcggcgtcc ggtagggcgg
      301 tcctcgagcg ctggagcgtg ccgttcgagc acgctgagca ccagatggcg ctcacgctca
      361 cggcgcgacc tggcccggtc agggaaggcc tggacctgtc cgaggcgcgc ctggccgacc
      421 tgccggaact ggcggccttg agcgctgcag ccttcgggat ggaccttcag tccaccgccg
      481 aggtcatggg gatgagcatc cgctcgggtg agcgcacgca gtgggtcttg cgtgacggtg
      541 gcaagccggt cggcctatgc gccaaggcgg agaaggacga cgcctacctg ctatacgggc
      601 tcgccatcca cccgtccgag cgccggcgcg ggtacgcgcg cgccatgcta gacctggtgg
      661 ccgggcgagc cttggatgcc ggggtcagga gcgtggtcct ggacgtggac agcgccaacg
      721 aggcagccat cgcgctgtac gaggggtacg gcttcgagca gacccagagc gcgctatatc
      781 acgcgttcga gttcgcccgg ttcgggggcg cgcagtgact cggcgtcctt ggcctggttc
      841 aagagctgtt cgatggcgcc gatcgcaagc gagttgatgc tgatcccgtc ctgctcggat
      901 ctctgagcca acctgcggtg caggcccggc tccatccgga tgaccacctt gccggagtac
      961 ggcttctccg gcgcttcccc aagctcgcgg caccactcga ggtactcgtc tacggagtcg
     1021 tggaaggctt gctcggcctc tcggacggtg cttccttgga aatggatcac gtccctgagg
     1081 ccgatgacca ctccgtccag gacgcccgcg tcaacgtcta tctccagttc ggccgtgtag
     1141 cccttgtacg gatcgagcct agtcatcaca gtcctccaga aatgctctca gccggtcgat
     1201 cagcttcttg ccacactcct tgcccgcgtg aggccggtgc acgatgagtt tcctgccgtt
     1261 cggggccgat atcctgaacc tcgatcctct cgtgcctatc actgagcacc ctagtgcctc
     1321 gagcaggccc gcgacctcgc tccaccggat gttcgcagcc gttggcgcgc cggcgatgcg
     1381 ccgcagcgtg tcaaggtgcc tgttgcgcaa cttgttggta gacactcgtt ccaggccctt
     1441 tcatcgatgc tagcacgtgg tgccagcatg tggggctccc tgccggcgga gagccgagtc
     1501 ggtagtctct tgagaacgcg tcctgctggc ttccagagtt atagaacggg gccgcttcag
     1561 cttctatggt cgagagccta gagtgcccag gcatagaatg ccgccatgtt cgctgtcgac
     1621 ctgcgctcag ataccgtcac cgttcccact tccgagatgc gcgccgcgat ggccgccgcg
     1681 gaggtgggcg acgacgtcta cgccgaagac ccgaccgtca accgcctgca ggagacggcg
     1741 gccgagctga cgggcttcga gggcgcgctg ttcgtcccct ccggcacgat gagcaaccag
     1801 atcgcgctgg
//
LOCUS       DAOQYE010000473         1799 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_78595_length_1799_cov_2.591743, whole genome shotgun sequence.
ACCESSION   DAOQYE010000473 DAOQYE010000000
VERSION     DAOQYE010000473.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78595_length_1799_cov_2.591743"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            25..450
                     /locus_tag="PKN52_07980"
     CDS             25..450
                     /locus_tag="PKN52_07980"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNQ99925.1"
                     /translation="MSNVRSPTAAADAVGLLDTSVVIRLPQLVQEDLPERPLISAITL
                     AELSVAPLVATTPQERAARQLVLQQAEADFEPLPFDAGAARAFGQVAASLRRDGRKVK
                     ARSYDAMIAAIALANRLPVYTANPDDFEGIDGLTVRVVQ"
     gene            complement(522..1649)
                     /locus_tag="PKN52_07985"
     CDS             complement(522..1649)
                     /locus_tag="PKN52_07985"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99926.1"
                     /translation="MLIGVIVIAIIFQSLNDRFLSTGNLVNLFVQASVFMLIGMGEVF
                     VLLLGEIDLSLGYVAGIGGVIVTELVQARTGWPWWSAVMTALLATGTIGFLQGSLVTR
                     LRLPSFVVTLAGLLGWQGVMIIILGTGGTIPIPNATINGIANGTIPPLAGWLLVAVVA
                     VVYAAITVRGDANRRLTGVAAPRPSTTVLKVLGVVAAAAVIALLTNANRGVGPYTLRG
                     MPYVIPLVFAVLLLWSTVLTRTRFGRYVYAIGGNAEAARRAGIALGWVRTLAFTFAGM
                     TAGVGGIVYASRLRSMSTSFDGGTIVLYVVATAVIGGTSLFGGRGHPLHAVLGGVVIA
                     AIVNGMALLGLSAAIQLIATAIVLLASIFVDVVIRRRGAAG"
BASE COUNT          311 a          594 c          640 g          254 t
ORIGIN      
        1 cgaccgcgtg ctggatgcgg cgttatgagc aacgtgcgct cgccgaccgc ggccgcagat
       61 gccgttgggc tgcttgacac gagcgtggtc atccggcttc cgcagcttgt gcaagaagac
      121 ctaccggagc gcccgctgat ctcggccatc acgctggccg aactctcggt agcgccgttg
      181 gtggcgacga ccccgcagga gcgcgccgcc aggcagttgg tactgcagca ggccgaggcc
      241 gacttcgagc cgttgccgtt cgatgcgggt gccgccaggg ctttcggtca ggtcgctgcc
      301 tccctgagga gggatggccg taaggtcaaa gcgcggagct acgacgcgat gatcgctgcc
      361 atcgcgctgg ccaatcggtt gcccgtctac accgccaacc ctgacgattt cgagggcatc
      421 gacggcttga ccgtgcgagt ggtccagtag ggcgccggtt ggtcgagtgc cgctcgcgag
      481 gccccgggcc gagaggcgcg cgaagcccat ctgaggaccg ctcaaccggc cgccccgcgg
      541 cgcctgatca cgacgtccac gaagatggac gcgagcagaa cgatggcggt cgcgatgagt
      601 tggatggccg cgctgaggcc aaggagcgcc atgccgttga cgatggccgc gatcaccacc
      661 ccaccgagca cggcgtgcag cgggtgcccg cgccctccga agaggctcgt gcctccgatg
      721 acggccgtgg cgactacgta caggacgatg gtgccgccgt cgaagctcgt cgacatggag
      781 cgcaggcgcg aggcgtacac gatgccgccc accccggccg tcatgccggc gaacgtgaac
      841 gcgagcgtgc ggacccaccc gagcgctatg ccggcgcgcc gagccgcttc ggcgttgccg
      901 ccgatggcgt agacgtagcg cccgaagcgc gtgcgcgtga ggacggtcga ccataacagg
      961 aggaccgcga acaccagggg gatcacgtaa ggcatcccgc gcagggtgta cggtccgacc
     1021 cctcggttcg cgttggtcag tagcgcgatt acggcggcgg cagcgaccac gccgagcacc
     1081 ttcaggaccg tggtcgaagg ccgtggcgcg gctacgcccg tgaggcgccg gttcgcatcg
     1141 cccctgacgg tgatggcggc gtagaccacg gcgacgacgg cgaccaacaa ccaacccgcg
     1201 agcggcggga tggtgccgtt ggcgatgccg ttgatggtcg cgttcgggat ggggatcgtg
     1261 ccgcccgttc cgaggatgat gatcatcacg ccctgccagc cgagcaggcc ggcaagtgtg
     1321 accacgaacg acgggagccg caggcgcgtg acgagactgc cctgcaggaa cccgatggtg
     1381 cccgtggcga ggagcgccgt catgacggcc gaccaccaag gccagcccgt gcgcgcctgg
     1441 acgagctccg tgacgatcac cccgccgata ccggccacgt agccgagcga gaggtcgatc
     1501 tcgccgagga ggagcacgaa cacctcgccc atcccgatga gcatgaagac ggacgcctgc
     1561 acgaagaggt tgacgaggtt gcccgtcgat aggaagcggt cgttgagcga ctggaagatg
     1621 atggcgatga cgatgacgcc gatcagcacg ggcaggacgt tcgtgccgcc agcgcccgtc
     1681 gtgcgcctct gggtacgggt ctcaggcgtg ccggtcgcat cggggacagg gcccctctcg
     1741 agttcggcgg cgtcctggac ggcggccaat taccgccatt acctggccgc ctactacca
//
LOCUS       DAOQYE010000474         1794 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_78974_length_1794_cov_2.543416, whole genome shotgun sequence.
ACCESSION   DAOQYE010000474 DAOQYE010000000
VERSION     DAOQYE010000474.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78974_length_1794_cov_2.543416"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="PKN52_07990"
     CDS             <1..531
                     /locus_tag="PKN52_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99927.1"
                     /translation="ELWVPDVGQNLFEEINVVTAPGNMGWNLNEGLHRFHPDDPNVVP
                     TDAAKTEGYMGEPLVAPIFEYTRERVDPDDFIGITVIAGYVYRGTQYPALQGHYVFGD
                     WGQSFGEPSGTLVVGEPQRAADGAVTGLVVGQVTRLDEFIMGFGEGADGELYVLTSAT
                     TGPQGTTGKVYRVVLD"
     gene            618..1238
                     /locus_tag="PKN52_07995"
     CDS             618..1238
                     /locus_tag="PKN52_07995"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HNQ99928.1"
                     /translation="MKIYTRTGDDGSTALFGGARVGKDDVRVEAYGTVDEANSALGLA
                     RTELVRGTAAGTVGGATGAAALAETLDADLAALQSLLFDLGADLATPMGTKTREFVRP
                     VDAEDVARIEEMIDHYTLELPALTHFILPGGTAAAAALHMARSIIRRAERSAVTLART
                     EEVGEQVLLLLNRLSDLFFTLARVACLRAGVSEVVWEARRPERPRR"
     gene            complement(1251..>1794)
                     /locus_tag="PKN52_08000"
     CDS             complement(1251..>1794)
                     /locus_tag="PKN52_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99929.1"
                     /translation="TIKTFLDQLRGQGVTDLAESYYAFRLSYRPPASVLGYELEFLHD
                     KAYYVTGLDPDHVVQHFELSDGHNVFFGNVTYTVDVGADSELVFRAGAGPAITNPATI
                     VRGEELGTRSEVDPGSRYYFTGVGAQLGVQGRYFLTDWLALSTETRYVFSWVRVPING
                     GDATATFNSVHVDFGLTFRP"
BASE COUNT          303 a          585 c          627 g          279 t
ORIGIN      
        1 gagctctggg tgcctgacgt cggccagaac ctcttcgagg agatcaacgt cgtgaccgcc
       61 cccggcaaca tgggctggaa cctcaacgag ggcctgcacc gcttccaccc ggatgacccg
      121 aacgtggtcc cgaccgacgc ggccaagacg gagggctaca tgggcgagcc gctcgtcgcc
      181 ccgatcttcg agtacacccg cgaacgcgtc gacccggatg acttcatcgg gataaccgtc
      241 atagccggct acgtgtaccg cggaacgcag tacccggcct tgcaaggcca ctacgtgttc
      301 ggcgactggg gccagagctt cggcgagccg agcgggaccc tggtcgtcgg ggagccgcag
      361 cgcgccgccg atggcgccgt caccggcctc gtggtcgggc aagtgacgcg gctggacgag
      421 ttcatcatgg gcttcggcga aggcgcagac ggtgagctct acgtgctgac gagcgcgact
      481 acaggcccgc aaggtacgac cggcaaggtc taccgggtag tgctcgactg agttccgcct
      541 cgttacgcag ttgatctcgt cgcctaccgg ccacttggcg gtgaccggta ggcgacgcga
      601 gcgcgtatcc tactcgcatg aagatctaca cccgcacggg cgatgacggc agcacggccc
      661 tgttcggcgg agcgcgcgtc ggcaaggacg acgtacgcgt cgaggcctac gggaccgtcg
      721 acgaggccaa ctcggcgctc gggttggcgc gtaccgagct cgtccgcggg accgccgccg
      781 gcaccgtcgg cggtgccaca ggtgcggccg cgctagctga gacgctcgat gccgacctcg
      841 ccgcgctgca gagcctgctc ttcgacctgg gcgccgacct cgccacgccc atgggcacca
      901 agacgcgcga gttcgtgcgc ccggtggacg cagaggacgt ggctaggatc gaggagatga
      961 tcgaccacta caccctggaa ctgccggccc tgacgcactt catcctgccg ggcggcaccg
     1021 ccgcggcggc cgcgctgcac atggccagat cgatcatccg acgagccgag cgcagcgctg
     1081 tgacgctggc tcggacggaa gaggtgggcg agcaggtgct gctgctcctc aaccggctct
     1141 cggacctgtt cttcactctg gcgcgcgtgg cctgtctgcg ggccggggtg agcgaggtcg
     1201 tctgggaggc caggcggccc gagagaccgc ggcgctgagg ccttaccgca ctacggtctg
     1261 aacgtcagtc cgaagtcgac gtggacggag ttgaaggtcg cggtcgcgtc accaccgttg
     1321 atgggaaccc ttacccacga gaagacgtag cgcgtctcgg tcgacaacgc gagccagtcc
     1381 gtgaggaagt acctgccctg cacgccgagc tgggcgccga cgcccgtgaa gtagtaccgg
     1441 ctgccggggt ccacctcgct gcgcgtgccc agttcctcgc ccctgacgat ggtggcgggg
     1501 ttggtgatgg ccggtccggc gccggcgcga aagacgagtt cggagtcggc gccgacatcg
     1561 accgtgtagg tcacgttgcc gaagaacacg ttatggccgt ccgagagctc gaagtgctgg
     1621 acgacatgat ccgggtcgag gccggtcacg tagtaggcct tgtcgtgcag gaactccagt
     1681 tcgtagccca gcacgctcgc gggtgggcgg tacgacaggc ggaacgcgta gtagctctct
     1741 gccaggtcgg tgacgccctg gccacgcagc tgatcaagaa aggtcttgat tgtg
//
LOCUS       DAOQYE010000475         1791 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_79181_length_1791_cov_2.628456, whole genome shotgun sequence.
ACCESSION   DAOQYE010000475 DAOQYE010000000
VERSION     DAOQYE010000475.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79181_length_1791_cov_2.628456"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1680
                     /locus_tag="PKN52_08005"
     CDS             <1..1680
                     /locus_tag="PKN52_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNQ99930.1"
                     /translation="RWWDLPTFFGTSYWDVPTRDLQLYRPLTIMSFAVDRAVFGPGPF
                     GVHLMNVLANSLIAALVYGLAWRLTQRRGLALAAALLFGLHPVHTEVVANGVGRAELY
                     AMLAVLGSTHLHLTHVRQTIGPTETGPAGRVGRPGCLVGALALYLVALLFKESAIVLP
                     GLLLLMEWLVLRAGRVRPMLPRIGTYALYALPLGVYLAARLAVVGFGSPAAQEVMADT
                     STLQRVLYVSETTWRYLGQLTLPLWLCAEYANYTKPIQPTLADPVTLAALVAWLGAAA
                     VIVWLIRRRQWLLLFGLAWFGLALLPVSNLLVPIGTVRADRLLFLPSLGFILLVAQLV
                     ADLARRQRVAGVALLVALLAFYGWRTVTRNRVWHDQTTFWTVTARQNPGSALAALMAG
                     AQHEEAGDFAAAEQAYQRAWELRDGAGFFYRDAHLKYAIRLQQRNERQAAAQHYRLVL
                     ERFPNDVTALVNLGQLLFGDDPTRTEAIQLLQRAVAIQPDDHVARFYLGLAYRRDDQR
                     TLALQEVDAAIALHPEQPDYWGAKALLLRELGRESEAAAAQQQAQRLRRRP"
BASE COUNT          252 a          699 c          547 g          293 t
ORIGIN      
        1 cgttggtggg acctgcccac cttcttcggc acgtcgtact gggacgtccc cacgcgcgac
       61 ctccagctct accgccccct gaccatcatg agcttcgccg tcgatcgggc cgtcttcgga
      121 cccggcccgt tcggcgtaca cctgatgaac gtactcgcca actccctgat cgccgcgctc
      181 gtatacggcc tggcctggcg gttgacgcag cggcgcggcc tcgcactggc ggcagcgctg
      241 ctgttcggcc tgcatccggt ccatacggaa gtcgtcgcca acggcgtcgg ccgggccgag
      301 ctgtacgcca tgctcgcggt cctcggcagc acccacctgc acctgacgca cgtccgccaa
      361 accatcggcc ccacggagac ggggccggcc gggcgtgtcg gccgacccgg ctgcctggtc
      421 ggcgcgctcg cgctatatct ggtcgcgctg ctgttcaagg agtccgccat tgtgctgccg
      481 gggctgctgt tgctgatgga gtggctcgtg ctgcgcgccg gccgggtgcg ccccatgctg
      541 ccgcgcatcg gcacctacgc cctctatgcc ctgccgctgg gcgtgtacct ggccgcccgg
      601 ctggccgtgg tgggcttcgg cagcccggcc gcccaggaag tcatggccga caccagcaca
      661 ctgcaacgcg tcctgtacgt cagcgagacc acttggcgct acctcggcca actgacgctg
      721 ccactctggc tatgcgccga gtacgccaac tacaccaaac ccatccaacc caccttggca
      781 gacccggtca cgctggccgc gctcgtggcc tggctcggcg ccgcggccgt tatcgtgtgg
      841 ctgatccgcc gccgccaatg gctgctcctg ttcgggctgg catggttcgg gctcgccctg
      901 ctgccggtca gcaacctgct cgtgcccatc ggcacggtcc gggccgaccg cttgctgttc
      961 ctgccgtcac tcggcttcat actgctcgtc gcccaactcg tggcggacct cgcccggcga
     1021 cagcgcgtcg ccggagtcgc actgctcgtc gcgctcctgg ccttttacgg ctggcgaact
     1081 gtcacgcgca accgcgtgtg gcacgaccag accaccttct ggacggtcac cgcccggcag
     1141 aatcccggca gcgccctggc cgcactgatg gccggcgctc agcatgagga ggccggcgac
     1201 ttcgccgccg ccgaacaggc ctaccaacgc gcctgggagt tgcgcgacgg cgccggtttc
     1261 ttctacaggg acgcgcacct caagtacgcc atccgcctcc aacagcgcaa cgagcgccag
     1321 gccgccgccc aacactaccg cctcgtgctc gaacggttcc ccaacgatgt caccgccctg
     1381 gtgaatctag gccagctcct cttcggcgac gaccccaccc gcacggaggc aatccagttg
     1441 ctccaacggg cggtcgccat ccagcccgac gaccacgtcg cccgcttcta cctcggtctc
     1501 gcctatcggc gcgacgacca gcgcacgttg gccttacagg aagtggacgc ggccattgcc
     1561 ctgcaccctg agcagccgga ctactggggc gccaaggcac tgctcctgcg cgaactcggg
     1621 cgtgagtccg aggccgctgc ggcccaacag caggctcagc ggctgcgccg tcgcccgtag
     1681 cgccgcagac ccgccgaagc cgaccaatcg gaacacctgg ttcagtagtc gcccgcgccg
     1741 cgcttggccc gctccagcgt acccaccact acccggatcg gctccggtgg c
//
LOCUS       DAOQYE010000476         1791 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_79196_length_1791_cov_2.296083, whole genome shotgun sequence.
ACCESSION   DAOQYE010000476 DAOQYE010000000
VERSION     DAOQYE010000476.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79196_length_1791_cov_2.296083"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="PKN52_08010"
     CDS             <1..538
                     /locus_tag="PKN52_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNQ99931.1"
                     /translation="IADLPGRKVLLRGNHDYWWPSISRLRRDLPAGMWALQNDAVLVD
                     GVVVSGTRGWVCPGSHGFTSEDQRIYDREVERLRLSLAHAAKLEGTYHVVMLHFPPTN
                     PRLEPSELTDLIIAAKPDALVFGHVHGEEPQGVLAHLDEVDVHFVAADALRFRPKLIA
                     EVGSAVVGVAALGAGGPA"
     gene            535..1719
                     /locus_tag="PKN52_08015"
     CDS             535..1719
                     /locus_tag="PKN52_08015"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HNQ99932.1"
                     /translation="MSVVAVDQGRRQVLVVNAGSSSLKMKLFPHGASLLVERIGGATA
                     AKATFALLEPPRLVDHAAAFAFALQVFATGVAGFAPAAVGHRVVHGGTRFVAPTLVTP
                     AVEIAIDELSRLAPLHNPGNLAALRAALAAMPDVPHVAVFDTAFHATLPPRAYLYGLP
                     LAYAEAGVRRYGFHGPSHDYVSRRAGELLGRDRSELRIVTLHLGNGASAAAVDRGRSV
                     DTTMGFTPMEGLLMGTRSGDIDPGVVLHLLRQGLSVHEADDLLNKGAGLKGMSGVSND
                     VRDVSAAAEAGSEGARAALDVFAYRVRKVVGAYAAVMGGLDAVVFTGGIGENAAELRA
                     ASLAGLEFLGIVVDDERNARGETSISTDDARVAVLVVKTDEELMIADAAAAVLNEPYE
                     ES"
BASE COUNT          254 a          624 c          630 g          283 t
ORIGIN      
        1 catcgccgac ctgccgggca ggaaggtgct gctgcgcggc aaccacgact attggtggcc
       61 gtccatcagc cgcttgcgtc gcgacttgcc cgcgggcatg tgggcgcttc agaacgacgc
      121 ggtgctcgtg gacggcgtgg tcgtgtccgg aacgcgcggc tgggtctgcc ctggcagcca
      181 cggcttcacc tcggaggacc agcgcatcta cgatcgcgag gtcgaacggt tgcggctctc
      241 gctcgcgcac gccgccaagc tggagggcac ctaccacgtg gtgatgcttc acttcccacc
      301 gaccaacccc cgcctcgagc cgtccgagct gacggacctg atcatcgcgg ccaagccgga
      361 cgccctcgtc ttcggtcatg tgcacggcga ggagccacag ggcgtcctcg cccaccttga
      421 cgaagtggac gtgcatttcg tggcggccga cgctctgcgc ttcaggccga agctcatcgc
      481 cgaggtgggg tccgcggtgg tcggcgtcgc cgcgctgggc gcgggcgggc cggcatgagc
      541 gtcgtggcgg tcgaccaggg tcggcgtcag gtgctcgtcg tcaacgccgg cagttccagc
      601 ttgaagatga agctgttccc gcacggcgcg tcgctgctcg tcgaacgcat cggcggggcc
      661 acggccgcca aggcgacgtt cgcgctcctc gagccgcccc gcctcgtcga tcatgccgcc
      721 gcgttcgcct tcgccctcca ggtcttcgcc accggcgtcg ccgggttcgc ccccgccgcc
      781 gtcggccacc gcgtcgtgca cggcggcacg cgcttcgtgg cgcccaccct cgtgacgcct
      841 gccgtcgaga tcgccatcga cgagctgagc cgcctggccc cactgcataa ccctggcaac
      901 ctcgcggccc tccgtgccgc gctcgccgcc atgcccgacg tgccgcacgt ggccgtgttc
      961 gacacggcct tccacgccac gcttccgccc agggcctacc tctacgggtt gccgctcgcc
     1021 tacgccgagg cgggcgtgcg gcgttacggc ttccacggtc cgagccacga ctacgtgtcg
     1081 cgccgggcgg gcgagctgct cgggcgcgac cgctccgaac tgcgcatcgt cacgcttcac
     1141 ctcggcaacg gcgccagcgc cgcggcagta gatcgcggcc ggtcggtcga cacgaccatg
     1201 ggcttcacgc ccatggaagg gcttctgatg ggcacgcgca gcggcgacat cgaccccggc
     1261 gtcgtgcttc accttctgcg gcaaggcctg agcgtccacg aggcggacga cctcctgaac
     1321 aaaggcgctg gcctcaaggg catgtcgggc gtctcgaacg acgtcaggga cgttagcgcc
     1381 gcggcggagg ccggttcgga gggtgcgcgg gcggcgctcg acgtgttcgc ctaccgagtg
     1441 cgcaaggtcg taggcgccta tgcggccgtg atgggtgggc tagatgcggt cgtgttcacg
     1501 ggcggcatcg gcgagaacgc ggcggagttg cgcgctgcct cgctcgctgg gctggagttc
     1561 ctcggcatag tggtggacga tgagaggaac gcgcgcggcg agacctcgat aagcacggac
     1621 gacgcgcgcg tcgccgtgct ggtcgtgaag accgacgagg aactgatgat cgcagacgcc
     1681 gccgcggcgg tgctgaacga gccatacgag gagagctgaa tggtcacgct aggtcttgac
     1741 ttgggaggaa cgaagatcct cgccgcgatc gtcgacgagg gcagggtgat c
//
LOCUS       DAOQYE010000477         1790 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_79247_length_1790_cov_2.966571, whole genome shotgun sequence.
ACCESSION   DAOQYE010000477 DAOQYE010000000
VERSION     DAOQYE010000477.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79247_length_1790_cov_2.966571"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..853)
                     /gene="trmFO"
                     /locus_tag="PKN52_08020"
     CDS             complement(<1..853)
                     /gene="trmFO"
                     /locus_tag="PKN52_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HNQ99933.1"
                     /translation="MEATHKRITVVGAGMAGSEAALAAARLGVAVDLIEMRPTKLTPA
                     HKTGDFAELVCSNSFGGEGESNAKGLLQAEMLKAGGVVMGSAHANRVPAGAALAVDRE
                     PFSRAVTAAVRTNPLITVHERELTKVPDGIVVLATGPLTADALATDIKDLLGDEFLGF
                     YDAAAPVIAYDSIDMSIAYRKGRYGQAADYINLPFTEADYDAFYAALESARSHTPHDW
                     EKLEFFEGCMPVEEIGRRGRDTLRFGPMKPVGLEHPETGKRPYAAVQLRQEDAEGRMW
                     SLVGFQTG"
     gene            990..>1790
                     /locus_tag="PKN52_08025"
     CDS             990..>1790
                     /locus_tag="PKN52_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99934.1"
                     /translation="MATSLRNVIARPLVAVVLALLAATALAAPQPWRAVYAGQLGGEY
                     VLVDLTRFDDGSAMARVLLHARGLSLTGRGLEAEDGTITIDLRATDQRNSPSFAVLYT
                     AQGDDPAASAGGDAAARLQAVRHDDWTDDGAVLSTSLTFTSATATSSDALEGDLARAA
                     QYAYMSLSEGRIDASTVWPRFSSAALDPIEHELEDDGIYHLRDFVIEGRDNVDALELG
                     WGWTQEVYSDVMGVAGPYVSILKSTTNYTGGAHPNSFYGSILFDTDTRT"
BASE COUNT          267 a          638 c          613 g          272 t
ORIGIN      
        1 ggcccgtctg gaagccgacg agcgaccaca tgcgcccctc ggcgtcctcc tgcctgagtt
       61 gcacggcggc gtaggggcgc ttgcccgtct cggggtgctc gagcccgacg ggcttcatcg
      121 ggccgaagcg gagcgtgtcg cggccgcggc ggccgatctc ctcgacgggc atgcacccct
      181 cgaagaactc gagcttctcc cagtcgtgcg gcgtgtgcga gcgggcggac tccagcgcgg
      241 cgtagaaggc gtcgtaatcg gcttccgtga acgggaggtt gatgtagtcg gcggcctggc
      301 cgtaccgccc cttgcggtag gcgatgctca tgtcgatcga gtcgtaggcg atgacgggcg
      361 cggcagcatc gtagaagccg aggaactcgt cgccgaggag gtccttgatg tcggtcgcca
      421 gcgcatctgc cgtcagcggg ccggtggcga ggacgacgat gccgtcgggc accttagtca
      481 gctcccgctc gtggacggtg atgagggggt tggtgcgcac ggcggctgtc acggcgcggg
      541 agaacggttc acggtcgacc gccagcgcgg caccggccgg caccctgttg gcgtgggcgc
      601 tgcccatcac gacgccgccc gccttgagca tctcggcctg caggagaccc ttggcgttgc
      661 tctcgccctc gcccccgaag gagttcgagc agaccaactc ggcgaagtcg cccgtcttgt
      721 gcgccggggt gagcttcgtc gggcgcatct cgatcaggtc gaccgccacc ccgaggcggg
      781 cggcggccag cgcagcttcg ctgccggcca tgccggcgcc gacgaccgtg atgcgcttgt
      841 gggttgcttc catgcgggtc agcgtagcac cggccggtgc tcgtggccgc agcggcggcc
      901 cgccccgccc cttcacgttc cgtgaaccta ggtagaccgc gcgcctacgg cgtacttggg
      961 ccctcacatg cgcgcattac acttgcccca tggccacttc cctacgcaac gtcatcgcgc
     1021 ggccgctggt agccgtggtc ctcgcccttc tggcagccac ggccctggcc gctccacaac
     1081 cgtggcgcgc cgtgtacgca ggccaactcg ggggcgagta cgtgctggtg gacctcacgc
     1141 gcttcgacga cggctccgcc atggcgcgag tgctgctgca tgcgcgcggc ctctcgctca
     1201 ctggccgcgg cctggaagcg gaagacggca ccatcaccat cgacctgaga gccaccgacc
     1261 agcgcaacag cccgagcttc gccgtcctct acaccgccca aggggacgac ccggcggcga
     1321 gcgccggcgg cgacgcggcg gctcgcctac aggccgtcag gcacgacgat tggacggacg
     1381 atggcgccgt cctgagcacc tcgctgacct tcacatcggc cacggcgacc tccagcgacg
     1441 cgttggaggg cgacctcgcg cgcgccgcgc agtacgccta catgtcgctc tcggagggcc
     1501 ggatagacgc cagcacggta tggccgcggt tctccagcgc cgcgctcgac cccatcgagc
     1561 acgagctcga ggacgacggc atctaccacc tccgggactt cgtgatcgag gggcgcgaca
     1621 acgtggacgc cctcgagctg ggctggggct ggacacagga ggtctacagc gacgtcatgg
     1681 gcgtcgcagg cccctacgtg agcatcctca agagcaccac caactacacg ggcggggcgc
     1741 acccgaactc cttctacggc tcgatcctct tcgacacaga cacgcggacc
//
LOCUS       DAOQYE010000478         1790 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_79268_length_1790_cov_2.204611, whole genome shotgun sequence.
ACCESSION   DAOQYE010000478 DAOQYE010000000
VERSION     DAOQYE010000478.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79268_length_1790_cov_2.204611"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            94..1581
                     /locus_tag="PKN52_08030"
     CDS             94..1581
                     /locus_tag="PKN52_08030"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNQ99935.1"
                     /translation="MRGSRPNLLLVITDQQRADTVTPGGPCLTPNLQRLAAEGARFER
                     CYAANPICSPSRASLFTGHLPHSHGVTDVTHAVPAPEADLRPGMPFWSRALARAGYRT
                     GYFGKWHVERTDRLEEYGFGEYETELRLVGVQEHRGELARSLRVSRSGYRDLLIAGVT
                     EARADQTHEHALVTKGVEFIERAVPGEPWALVVSTEAPHDPYIAPREYFERYDPAAIA
                     EPASFADPMTDKPAIYRRIREAWRDLAWDDYAFATACYLGLCSMLDDQVGRLLAALDA
                     TGQADDTLVVFTSDHGDYLGAHGLLLKGVPAFEEAYRVPLVMRGPGVAPGVSVPGPVS
                     LLDLPRTLVRLLLGEEFDCQGGELTGWLTGERDPGGEVGQLPVGAAPAGAGPVGDTPV
                     RRSASAPGEAFAEFHGQRFRYTQRVLWRGDWKYVMNGFDQDELYDLASDPHELRNLAA
                     DPEHGPVLEDMARRMWRWVKDTGDETLAEAHYGMFRFLPVGPGAG"
BASE COUNT          254 a          652 c          629 g          255 t
ORIGIN      
        1 ggcgttctcg ctcgcgttcg ccgcccaggc gctctggctc cgccaccgca gccagcccct
       61 cgtgcgcgcg gcgctgaggc ggggggcggt gcggtgaggg gctcccgccc caacctcctc
      121 ctcgtcatca cggaccagca gcgcgccgac acggtgacgc ccggcggccc gtgcctcacg
      181 cccaacctcc agcgcctggc cgcggagggc gcgcgcttcg agcgctgcta cgccgcgaac
      241 cccatctgct cgccctcgcg cgcgagcctc ttcaccggcc atctgcccca cagccacggg
      301 gtgacggacg tgacgcacgc cgtgcccgcc cctgaggccg acctgcgccc cggcatgccg
      361 ttctggtcgc gcgcgctggc acgcgccggg tatcgcaccg gttacttcgg caaatggcac
      421 gtggagcgca ccgaccgcct cgaggagtac gggttcggcg agtacgagac ggagctgcgg
      481 ctcgtcgggg tccaggagca ccgcggcgag ctggcgcgct ccctgagggt ctcccggtcc
      541 ggctaccgcg acctcctcat cgccggcgtg acggaggcgc gggccgacca gacgcacgag
      601 cacgcgctcg tcacgaaggg cgtcgagttc atcgagcgag ccgtgccggg cgagccgtgg
      661 gcgctcgtcg tgagcaccga ggcgccgcac gacccgtaca tcgcgccgcg cgagtacttc
      721 gagcgctacg acccggccgc catcgccgag ccggcgagct tcgcggaccc catgacggac
      781 aagcccgcca tctaccgccg catccgcgag gcgtggcgcg acctcgcgtg ggacgactac
      841 gccttcgcca ccgcctgcta cttaggccta tgcagcatgc tcgacgacca ggtcggtcgc
      901 ctcctcgccg cgctcgatgc cacgggccag gcggatgaca cgctcgtcgt cttcacgtcc
      961 gaccacggcg actacctggg cgcgcacggc ctgctcctca agggcgtgcc ggccttcgag
     1021 gaggcgtacc gcgtgcccct cgtcatgcgc ggtcccggcg tcgcccccgg cgtgagcgtt
     1081 cctggcccgg tcagcctcct cgacctgcca cgcacgctcg tgcggctgct cctcggcgag
     1141 gagttcgact gtcagggcgg ggagttgacg gggtggctga cgggcgagcg cgaccccggg
     1201 ggtgaggtcg gccagctacc ggtaggggcg gccccagcag gcgctgggcc ggtcggtgat
     1261 acgccggttc gacggtccgc tagcgccccc ggcgaggcgt tcgccgagtt ccacggccag
     1321 cgcttccggt acacgcagcg cgtcctgtgg cgtggcgact ggaagtacgt catgaacggc
     1381 ttcgaccagg acgagctcta cgacctcgcg agcgacccgc acgagctgcg caacctggca
     1441 gccgaccccg agcacgggcc cgtgctggag gacatggcgc ggcgcatgtg gcgctgggtg
     1501 aaggacaccg gcgacgagac cctggccgag gcgcactacg gcatgttccg gttcctgccg
     1561 gtggggccgg gtgcggggta gccaagccgg ctggaagata gccaagtcgg ctagaacatg
     1621 tacaagacct cggctgtctt gtatcatatc ggtgtgaagg cgatcgatgt cagcacagcc
     1681 cgtgccagcc tagcggaagt tctggacgcc tcccgcgagg gagacgtggt gatcgagcgg
     1741 tatggcaagc ctgccggtgt cctcatcagc cccgagcggc ataagcagtt
//
LOCUS       DAOQYE010000479         1789 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_79300_length_1789_cov_3.464245, whole genome shotgun sequence.
ACCESSION   DAOQYE010000479 DAOQYE010000000
VERSION     DAOQYE010000479.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1789
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79300_length_1789_cov_3.464245"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="PKN52_08035"
     CDS             complement(<1..261)
                     /locus_tag="PKN52_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99936.1"
                     /translation="MTRSSSNSIGREWDLAVRLAEHVGSGGEVPVLASPVLLDPSEVL
                     HADLWADGWRFLAMDVFYDEPRIVAFGGPLLFATTALSAGSAR"
     gene            complement(419..1674)
                     /locus_tag="PKN52_08040"
     CDS             complement(join(419..1369,1372..1674))
                     /locus_tag="PKN52_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611735.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HNQ99937.1"
                     /translation="MPQEKSPGKPTTRRYSDQEKEQAVRLVRQARSEGRGHGAIQRIA
                     LQLGYGTETVRKWVKVADIDEGVKPGRSTADAAKIKELEAQVKELKRANEILRKASAF
                     CPGGARPPVNVLVGFIDEHRDELGVEPICKALQVAPSSYYAAKKRELAPSARAVRDAV
                     MMQVLMVLWVTNRKVYGAHKLWLAARRAGHEIGRDQVARLMRELGIQGVSRRRKKVFS
                     TISDPDATRAADLVKRNFTADRPDALWVTDLTYVPTRSGMAYVCFIVDAFSRRIVGWR
                     AASHMRTDMVLDAVEMARRSRGGGRLVGLVTHSDAGSQFTSVRFTERLDEIGARPSIG
                     TVADSYDNALAETTNGLYKAECVYGPDTTGWADVNELEHATLDWVYWFNEQRLHSHCG
                     HVPPAEFEAAFYAAQQADPVGVGNQ"
     misc_feature    complement(1259..1375)
                     /locus_tag="PKN52_08040"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01497"
BASE COUNT          289 a          620 c          564 g          316 t
ORIGIN      
        1 ccgcgccgaa cccgctgaca gcgctgtcgt ggcgaacaga agcggcccac cgaacgcaac
       61 gatccgtggc tcgtcgtaga agacgtccat cgccaggaac cgccacccat cggcccacag
      121 gtccgcatgg aggacctcgc tgggatcgag gagaaccgga ctggcgagga ccggaacctc
      181 gccaccggaa ccgacgtgct cggcgagtcg aaccgcgagg tcccactctc ggccgatgga
      241 gttgctactg cttcgagtca tgttggtcac ctcctgctgc ggaaccctga agtggcctac
      301 ctgacgcggc ggacccggac tccgcggctc atcccctcaa cgaacagttc gtcacgttcg
      361 aggcgggcct gcgggcggta ggcgtagtga accaccctgg gtctgatgga ggctcgttct
      421 attggtttcc aaccccgacg gggtcggctt gttgggcagc gtagaacgct gcttcgaact
      481 ccgccggggg cacgtggccg cagtggctgt ggagccgctg ttcgttgaac cagtacaccc
      541 agtcgagcgt cgcgtgttcg agctcgttga cgtcggccca gcctgtggtg tcgggcccgt
      601 agacgcactc agctttgtag agaccgttgg ttgtctcggc cagggcgttg tcgtaggagt
      661 cggcgacggt gccgatcgaa gggcgggctc cgatctcgtc gagccgttcg gtgaaccgca
      721 cactggtgaa ctggctgccg gcgtcggagt gggtgacgag cccgacgagg cgccctccgc
      781 ctctggatcg tctggccatc tcgacggcgt cgaggaccat gtcggtacgc atgtgcgacg
      841 cggcgcgcca gccgacgatc cgacgactga acgcgtcgac gatgaaacac acgtacgcca
      901 tcccggaccg ggtcggcacg taggtcaggt ccgtgaccca caacgcgtcg ggccggtccg
      961 cggtgaagtt gcgcttcacc aggtccgctg cccgggtggc gtccgggtca gagatggtgc
     1021 tgaacacctt cttgcgacgc cgagagacgc cctggatgcc cagctcgcgc atcagacggg
     1081 cgacctggtc ccggccgatc tcatggccag cgcgtctggc tgccagccac agcttgtgag
     1141 cgccgtagac cttgcggttc gtcacccaca acaccatcag cacctgcatc atcaccgcgt
     1201 cacgcaccgc tctcgccgag ggggcgagct cgcgcttctt ggcggcgtag tagctgctcg
     1261 gagccacctg cagggccttg cagatgggct cgactccgag ctcgtcacgg tgctcgtcga
     1321 tgaacccgac aagcacgttc acgggcggtc gagctccgcc tgggcaaaat acgccgacgc
     1381 cttccgcaag atctcgttcg cccgcttcag ctccttgacc tgagcctcga gctccttgat
     1441 cttcgccgcg tccgcggtcg accgtcccgg cttcacaccc tcatcgatat cggcgacctt
     1501 gacccacttg cgcaccgtct cggtcccata tccgagctgt agcgcgatcc gctggatcgc
     1561 gccatgacca cgtccctccg aacgggcctg gcgcaccaga cggaccgcct gttccttctc
     1621 ctgatccgag tagcgacgcg tagtcggctt ccccggcgac ttctcctgtg gcatgactcc
     1681 agccttccaa gctcaggagc ctccaacgaa cccagggtgg ttcaccgcgg cagctcgcga
     1741 cggcgtcgct gccgccatcc ggtccgcgct tgcgcaagcc gaccgcgat
//
LOCUS       DAOQYE010000480         1787 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_79468_length_1787_cov_2.791570, whole genome shotgun sequence.
ACCESSION   DAOQYE010000480 DAOQYE010000000
VERSION     DAOQYE010000480.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79468_length_1787_cov_2.791570"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            338..1117
                     /locus_tag="PKN52_08045"
     CDS             338..1117
                     /locus_tag="PKN52_08045"
                     /inference="COORDINATES: protein motif:HMM:NF016836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNQ99938.1"
                     /translation="MWPFGKNAQGRLEDALKDQELTAKLGLKVSVKDKVAAISGQVPN
                     ERYKNLLKALATGINGIEDVDLSGITVADGGQGAPAQTGTMASPIQGTAQAGAAQAGA
                     AQAGAAQAADAVDPSALAKAALTGIKQEPSLQNNPLDVLQKGSTVVLRGAVDDQGEFD
                     KARAIALAVPGVTGVDVSGLQVIANASALNVTDKDGDIVYTVKSGDTLSHIALKYYGS
                     AGRTSYMKIAEANGIADPNKIRVGQVLKIPGTTQGPDQVLA"
     gene            complement(1279..>1787)
                     /locus_tag="PKN52_08050"
     CDS             complement(1279..>1787)
                     /locus_tag="PKN52_08050"
                     /inference="COORDINATES: protein motif:HMM:NF015596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99939.1"
                     /translation="LASLRPANRLRKFAAGALLFGALTLAALAIAQAVAPASLAVATA
                     EDGTVHVVDADGNSLYLFLNDFNNVSSCDAGCAVNWPPALAVDASTLPAVSDELDASL
                     LGTTERADGMIQLTYNGWPLYYFAGDGRPGDATGQGINDVWFLVTPEGTGAGLAADAL
                     GGGAAPMQ"
BASE COUNT          313 a          595 c          610 g          269 t
ORIGIN      
        1 cggttcttgc gacggggcgg tgtgccccgg gaagcggccc tcgacgactt gccggccatg
       61 gccgcctcga acgcgcccta ctggtgtgcg ctgctcgaga tcccgctacg cgacgaggac
      121 ttacgcgtgg actgcccgga ctaccggccg aaatctactt gaacgcagcg ccgacgacgc
      181 ggtcgtggcg cgccggcgtt acggtcgggc tgcgcccgcc gtgtcgaccg gcgggcctcg
      241 tggctttctg ccttgctaaa gtcgagtgtt tgaagtgcca acgccacgac cttgactgca
      301 gggtctgggt tgaacggcgc aaacggaagg gaacgacatg tggccattcg gtaagaacgc
      361 gcagggccgg ctcgaggacg ctctgaaaga ccaggagctc accgcgaagc tcggtctcaa
      421 ggtaagcgtg aaggacaagg tcgccgccat ctcgggccag gtcccgaacg agcggtacaa
      481 gaacctcctc aaggccctgg cgacaggcat caacggcatc gaggacgtcg atctgagcgg
      541 catcacggtg gccgacgggg gtcaaggcgc cccggcccag accggtacca tggcgtcgcc
      601 catccagggc accgcgcaag ctggtgccgc ccaagctggt gccgcccaag ctggtgccgc
      661 ccaagcggca gatgcagttg accccagcgc cctcgccaag gctgccctga ccggcatcaa
      721 gcaggagccc agcctccaga acaacccgct cgacgtcctc cagaagggca gcacggtcgt
      781 cctgcgcggc gcggtcgacg atcagggcga gttcgacaag gctcgcgcca tcgctctcgc
      841 ggttcccggt gtcaccggcg tcgacgtctc gggcctgcag gtgatagcga acgcttccgc
      901 gctcaacgtc accgacaagg acggcgacat tgtctacacg gtcaagtccg gcgataccct
      961 ctcgcacatc gcgctcaagt actacggcag cgcgggccgc acgagctaca tgaagatcgc
     1021 agaggccaac ggcatcgccg acccgaacaa gatccgcgtc ggtcaggtcc tgaagatccc
     1081 cggcaccacc caaggtcccg atcaggtctt ggcctaagcc tgtccgggcc gaactcggac
     1141 cgtaaagagc acggaatatg agaggcgccc gctgtgatgc gggcgcctcg agtctttggg
     1201 ctggccggta gtcgtccgga gcggaacccc tggccccggc taatgccggg gaagaagagg
     1261 acccggcgtg cggagcgctt actgcatcgg tgccgcgcca ccgcccagtg cgtccgcggc
     1321 gaggcccgcg cccgtgcctt cgggggtcac gaggaaccag acgtcgttga tgccctgacc
     1381 ggtcgcgtcg ccgggtcggc cgtcgccggc gaagtagtag agcggccacc cattgtaggt
     1441 gagctggatc atgccgtcgg cgcgctcggt ggtgccgagc aggctggcgt caagctcgtc
     1501 cgagacggcc gggagggtgc tcgcatcgac cgctagcgct ggcggccagt tcacggcgca
     1561 accggcgtcg cagctgctca cgttgttgaa gtcgttcagg aacaggtaga gcgagttgcc
     1621 gtcggcatct accacgtgga cggtcccgtc ctctgccgtc gcgacggcca ggctcgccgg
     1681 cgcgacagct tgggctatcg ccagggccgc gagtgtcaag gccccgaaca gcagggctcc
     1741 agctgcgaac ttcctgaggc ggttggctgg gcggaggctt gctagcg
//
LOCUS       DAOQYE010000481         1780 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_79977_length_1780_cov_2.894493, whole genome shotgun sequence.
ACCESSION   DAOQYE010000481 DAOQYE010000000
VERSION     DAOQYE010000481.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1780
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79977_length_1780_cov_2.894493"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /locus_tag="PKN52_08055"
     CDS             complement(<1..713)
                     /locus_tag="PKN52_08055"
                     /inference="COORDINATES: protein motif:HMM:NF019236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HNQ99940.1"
                     /translation="MRALVPRLMLPLMLLLSACSGGPAGPPGPLFVNPAAGSDAATGT
                     AARPLKTIGRALELVKAGGEVFLQVGTYRGEAWPLAVPAGVTIASVAPGGAVLASSIA
                     GEGKALAFAQGGAATDLTIEDFAVGVSVTGGTVELVGLRFTRIATISVVALGDSDVTL
                     QGCVFQSTGSAVGVDAREDATVSVDGGSVAGGGAAFRVWAQVRFSARNLTVDDSEAGL
                     WNPSGSGSASLTGVAISNTR"
     gene            complement(833..>1780)
                     /locus_tag="PKN52_08060"
     CDS             complement(833..>1780)
                     /locus_tag="PKN52_08060"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNQ99941.1"
                     /translation="TWPTLVGGVLDPGPNVPAGVLVQGQSTARVVLKGPGSAGSPFAV
                     GLAFADAGGAGGLTAESFAAGFFVTAGDVVLQAVRAHGNVVGLAVDGGQVIAHSSEFS
                     ANGEDGVLVVGGAHVELEGGSAHDNAAAGVRLGDGAPTLQTDGFEAYGNLTGVAVAGD
                     ASAVLEDSEFHHNSDHGLEVIESGSAEARGSELHANTQAGLWFGGKSLIVRDTTVHDN
                     GEFGVYVEGEPEKADFGNFSEAGNNLLYDNGPGTADQLLDVRADRPGLGDPEVFTMSA
                     TSLAGETPAPDIYPPDGMWPYFNSPHFSILGENNFIHVY"
BASE COUNT          273 a          634 c          584 g          289 t
ORIGIN      
        1 cgcgtgttgg agatggccac gccggtcagg ctggccgacc cggagccgct cgggttccac
       61 agcccagcct cgctgtcgtc gacggtcagg ttgcgcgcgc tgaaacgcac ctgagcccat
      121 accctgaacg cagcgccgcc acccgccacg ctcccgccgt cgacggacac cgtcgcgtcc
      181 tccctggcgt cgacgcccac ggccgatccc gtgctctgga acacgcaccc ctgcagtgtc
      241 acgtcgctgt cgccaagagc cacgacgctt atggtcgcga tcctggtgaa gcggaggccg
      301 accagttcga cggtgccacc cgtcaccgac acccccacgg cgaagtcctc gatagtcaag
      361 tccgttgccg cccctccttg ggcgaacgcc agggccttgc cttcacctgc gatagagctg
      421 gccaggaccg cgccgccggg cgcgaccgag gcaatggtga ccccggccgg tacggcgagg
      481 ggccacgctt cgccccggta cgtcccgacc tgcaggaaga cctcaccgcc ggccttgacg
      541 agctcgagcg cgcgtccgat ggtcttgagg ggccgcgccg ccgtgccagt tgcagcgtcg
      601 gaacctgccg cggggttgac gaacagcggt ccgggcgggc cagcaggacc gccgctgcac
      661 gcgctcagca gcagcatgag cggcaacatc agacgtggaa cgagggcgcg catcgctgct
      721 ccttactagg tgggggaaac ccagcgaatc ataccgccgc cagcggtctt ggcgttgcct
      781 ggcggggcga cgccaacgag gtggccctca gggcatggcc ctgctgcata tttcaataca
      841 cgtgaatgaa gttgttctcc ccgaggatcg agaagtgggg cgagttgaag tacggccaca
      901 tgccgtctgg cggatagatg tcgggggctg gcgtctcgcc ggccaacgac gtggcgctca
      961 tggtgaagac ctccgggtct ccgagcccag ggcggtcagc gcgtacgtcg aggagttggt
     1021 cggctgtgcc cggcccgttg tcgtagagga ggttgttgcc cgcctccgag aagttcccga
     1081 agtcggcctt ctccggttcg ccttccacgt agacgccgaa ctcaccgttg tcgtgaacgg
     1141 tcgtgtcgcg cacgatcagg ctcttaccgc cgaaccacag cccggcttgg gtgttggcgt
     1201 gtagctcgct tccgcgcgct tcggcgctgc cactctcgat gacctcgagg ccgtgatcgc
     1261 tgttgtggtg gaactcgctg tcttcgagaa cggcgctcgc gtcgcccgcc acggcgactc
     1321 cggtcaggtt gccatatgcc tcgaagccgt cggtctgaag cgtcggggcg ccgtcaccca
     1381 ggcgcacgcc tgcggccgcg ttgtcgtgcg cggagccccc ctcgagctcg acgtgtgctc
     1441 cgcccaccac cagcaccccg tcctcaccgt tggcgctgaa ctcgcttgag tgggcgatca
     1501 cctgtccgcc gtccactgcc aacccgacca cgttgccatg agcccggacc gcttgcagta
     1561 cgacgtcgcc tgcggtcacg aagaagccgg ccgcgaagct ctctgccgtc agaccgccgg
     1621 ccccgccggc gtcggcgaac gctagtccga ccgcgaatgg cgaacccgcg cttcccgggc
     1681 ccttgagaac gacgcgggcc gtgctctggc cctgtacgag cacgcctgct ggaacgttgg
     1741 gcccgggatc gagtacgccg ccaacgaggg tcggccaagt
//
LOCUS       DAOQYE010000482         1779 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_80075_length_1779_cov_2.470418, whole genome shotgun sequence.
ACCESSION   DAOQYE010000482 DAOQYE010000000
VERSION     DAOQYE010000482.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80075_length_1779_cov_2.470418"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1352
                     /locus_tag="PKN52_08065"
     CDS             <1..1352
                     /locus_tag="PKN52_08065"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HNQ99942.1"
                     /translation="GLIDDIWVDRPADAPAAIVDHPVEFAGEAASSKLQRLRHRLAEL
                     GATSMLVTALDEVAWLLNLRGNDIPMNPVALAYCVVGPDNVQLFAHEEQVSPGLRAAL
                     PAEVVIRAYADVAAGLKELPVNTSLLLDPAKTSVLLYEAASHLQRVEGPSPLDAMKAR
                     KNETELRCALLAHVYDGVAVTRLLHWLSTTDLNAETELSVSEKLQYFREGLPDYRGAS
                     FDTIVGFGPNSSVGHYKLNRENPQPLAPESLLLIDCGAQFLTGTTDTTRTVALGTPTA
                     EHKRTYTTVLKSLIALSAARFPKGTTGQKLDAIGRSHIWANDWECRHGIGHGVGSYLH
                     VHEGPQRINKTNDVVFEVGHVNSNEPGVYFEGVFGVRLENVIAVAPAGTSVFGEFLRF
                     DTLTPIPFDRDLIDVDLLTSQEVDWLDAYHHRVEETLSPHLPAGVRGWLAERAAPLR"
     gene            complement(1387..>1779)
                     /locus_tag="PKN52_08070"
     CDS             complement(1387..>1779)
                     /locus_tag="PKN52_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99943.1"
                     /translation="SCGLGKRSPTGSFDYTGTWRGSLTDETSGAGAFMTTLQQVDFVV
                     TGTWHSVMGSDAARQDGGALSGQLFVGKDSDLLEVTLSPAVAGRCSYKLTLSRAQETI
                     SGSYVPVGSTAECGDLTRGTVQVSKQQQ"
BASE COUNT          299 a          644 c          560 g          276 t
ORIGIN      
        1 ccgggctaat cgatgacatc tgggtggacc gccccgccga cgcgccggcg gccatagtcg
       61 accaccctgt cgagttcgcg ggcgaggccg cctcgagtaa gctccagcgc ctgcgtcacc
      121 gccttgccga actgggcgcc acgagcatgc tcgtaacggc cctcgacgaa gtggcgtggc
      181 tactgaacct gcgcggcaac gacataccga tgaacccggt cgcgttggcc tactgcgtcg
      241 tcggaccgga caacgtacag ctcttcgcgc acgaggagca ggtctcgccc gggctgcggg
      301 ctgccctgcc cgccgaggtc gtcatccgcg cgtacgcgga cgtcgccgcc ggactcaagg
      361 agctacccgt caacacgagc ctcctcctcg acccggccaa gacgagcgtt ctcctatacg
      421 aggccgcgag ccacctccag cgagtcgagg gccccagccc gcttgacgcg atgaaggcga
      481 ggaagaacga gacggagctg cgctgcgccc tcctcgccca cgtgtacgac ggcgtcgcgg
      541 tcacgcggct actgcactgg ctgagcacta ccgatctgaa cgccgagacc gagctttccg
      601 tctccgagaa gctccagtac ttccgcgagg gccttcccga ctaccgtggc gcgagcttcg
      661 acaccatcgt gggtttcgga ccaaactcgt ccgtcggtca ctacaagctg aaccgcgaga
      721 acccgcagcc gctagccccc gagtcgctcc tcctgatcga ctgtggcgcc cagttcctca
      781 cgggcaccac cgacacgacc cgcaccgtcg cactcggcac gcccaccgcg gagcacaagc
      841 gcacctacac cacggttctg aagagcctca tcgcgctcag cgccgcacgc ttccccaagg
      901 gcacgaccgg ccagaagctg gacgccatcg ggcgctcgca catctgggcg aacgactggg
      961 agtgccgcca cggcatcggc cacggcgtcg gcagctacct acacgttcat gaggggccgc
     1021 agcggatcaa caagacgaac gacgtcgtct tcgaggtcgg gcacgtgaac tccaacgagc
     1081 cgggcgtcta cttcgagggc gtgttcggcg tgcgcctcga gaacgtgatc gccgtggcgc
     1141 cggccggcac gagcgtcttc ggcgagttcc tgcgcttcga cacgctcacg ccgatcccat
     1201 tcgaccggga cttgatcgac gtcgacctgc tcacgagcca ggaagtggac tggctcgacg
     1261 cgtaccacca ccgggtcgag gagacactaa gccctcatct gccggcgggg gtgcgcggct
     1321 ggttggcgga gcgggcagcg ccgttgcgct aggcgcctac ggtcagccat ctaccgccct
     1381 agccgcctac tgttgctgct tgctgacctg caccgtgccg cgcgtgagat cgccacactc
     1441 ggcggtcgag ccgacgggca cgtaactgcc gctgatcgtc tcttgtgcac gcgacagcgt
     1501 cagcttgtac gagcaccggc cggccacggc cgggctgagg gtgacctcga gcaggtcgct
     1561 gtccttgcct acgaacagct ggccgctcag ggcgccgccg tcctgccgag ccgcgtcgct
     1621 ccccatgacg ctgtgccatg tgcctgtgac gacgaagtcg acctgctgca gcgtggtcat
     1681 gaaagctccg gcgccgctcg tctcgtctgt gaggctgccg cgccaggtgc ccgtgtagtc
     1741 gaacgagccg gtcggggacc tcttgccgag cccacaact
//
LOCUS       DAOQYE010000483         1778 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_80145_length_1778_cov_2.968659, whole genome shotgun sequence.
ACCESSION   DAOQYE010000483 DAOQYE010000000
VERSION     DAOQYE010000483.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1778
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80145_length_1778_cov_2.968659"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="PKN52_08075"
     CDS             <1..286
                     /locus_tag="PKN52_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99944.1"
                     /translation="LGSSVLYFSFEVAANYALLHAVINLVPILPLDMGRAALAWGHPD
                     VRRVIMQVAQFGILGFMVFFFVLNATGVIGRVMFFFDRLFSGIIGLIPGL"
     gene            complement(288..>1778)
                     /locus_tag="PKN52_08080"
     CDS             complement(288..>1778)
                     /locus_tag="PKN52_08080"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNQ99945.1"
                     /translation="EGYIEGHELDIHLSVEIMQRVVRALQHIHSAGIIHRDIKPANVM
                     ITRGGIKPSESGHPEPGRLKPDAVKLMDFGIAGGKVLSRLTMTGARVGTPVYMSPEQA
                     RGLKIDHRSDIYSLGLVFYEMLTGQTAFKGGYEAIVHQQIFQTPPPPRQVDLRIPKAL
                     DQLVMRMIAKDPDERPTLDEVYDTLETVDLEADESANLPTRLLVTVSARQGVLRILDP
                     DGNLHTSIGDIGVGHGNFSAAPLAVSVDSGGDLFAAIFEYRVGQQSHRMIHKLAVDGS
                     VKASFGPYGMQPGEFLYPVALAVAPDDTVYVLDSETHLVSRFTNDGRYLASFGGRGNG
                     HGMFNDPRDVAVDVRGYVYVLDYGNRQVQRLSADGEYQTRWAFKVAADQPGLRLLDGM
                     TVDRDCNVYVSDASAGKIRKVTPDGKVGVSFTLDARQGEATDALVDLGVDDSGYLYAG
                     RRGGHLIRKYDPAGRLLQTFETYAPMVTMVVDVREPSPEPVSTLAG"
BASE COUNT          250 a          590 c          618 g          320 t
ORIGIN      
        1 cctcggcagc tcggtgctct acttctcctt cgaggtggcg gccaactacg ccctgctcca
       61 cgccgttatc aacctcgtcc cgatcctgcc gctcgacatg ggtcgcgccg ccctcgcctg
      121 gggccacccc gacgtgcgcc gcgtgatcat gcaggtcgcc cagttcggca tcctcgggtt
      181 catggtgttc ttcttcgtgc tgaacgccac cggcgtgatc gggcgcgtca tgttcttctt
      241 cgaccggttg ttctccggca tcatcgggtt gatacccggc ctgtaactca acccgccagc
      301 gtgctgaccg gctccggcga cggctcgcgc acgtcgacga ccatggtcac catgggcgcg
      361 tacgtctcga acgtctgtag caggcgcccg gccgggtcgt acttgcggat gaggtgcccg
      421 ccgcgccgac cggcgtacag gtagccggag tcgtcgacgc ccaggtcgac gagggcgtcc
      481 gtcgcctcgc cctggcgggc gtcgagcgtg aacgacacgc cgaccttccc gtctggcgtg
      541 accttgcgga tcttgccggc gctcgcgtcg gagacgtaga cgttgcagtc gcggtcgacg
      601 gtcatgccgt cgagcaggcg cagacccggc tggtcggcgg cgaccttgaa cgcccagcgc
      661 gtctggtact cgccgtcggc gctcaggcgc tgcacctgtc ggttgccgta gtcgaggacg
      721 tagacgtagc cgcgcacgtc gaccgccacg tcgcgcgggt cgttgaacat gccgtggccg
      781 ttgccgcgcc cgccgaaact cgccaagtaa cggccgtcgt tcgtgaagcg cgagaccagg
      841 tgcgtctcgc tgtcaaggac gtagaccgtg tcgtctggcg ccacggcgag ggccaccggg
      901 tagaggaact cgcccggctg catgccgtac gggccgaacg acgccttgac cgagccgtcc
      961 acggccagct tgtggatcat gcggtggctc tgctgaccga cgcggtactc gaagatggcc
     1021 gcgaagaggt cgccgcccga atcgacgctg acggcgaggg gcgccgccga gaagttgccg
     1081 tgcccgaccc cgatgtcgcc gatggacgtg tgcaagttgc cgtcgggatc gaggatgcgc
     1141 agcacgcctt ggcgcgcgct gacggtgacg aggaggcgcg tcgggaggtt cgcgctctcg
     1201 tcggcctcga ggtcgaccgt ctccagggtg tcgtagacct cgtcgagcgt cgggcgctcg
     1261 tccgggtcct tcgcgatcat gcgcatgacg agctggtcga gggccttggg gatgcgcagg
     1321 tccacctggc gcggcggcgg cggcgtctgg aagatctgct ggtggacgat ggcctcgtaa
     1381 ccgcccttga aggcggtctg gcctgtcagc atctcgtaga agacgagccc gagcgagtag
     1441 atgtctgagc ggtggtcgat cttcaggccg cgcgcctgtt cgggcgacat gtagaccggc
     1501 gtcccgacgc gagcgcccgt catggtcaga cgcgacagga ccttcccgcc cgcgatgccg
     1561 aagtccatga gcttgaccgc gtcgggcttc aggcgccctg gctcggggtg gccgctctcg
     1621 gacggcttga tgccgccgcg cgtgatcatc acgttggcgg gcttgatgtc gcggtggatg
     1681 atgccagccg agtggatgtg ctgcaaggcg cgcacgacgc gctgcatgat ctcgaccgac
     1741 aggtgaatgt ctagctcgtg cccctcgatg taaccctc
//
LOCUS       DAOQYE010000484         1778 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_80163_length_1778_cov_2.528729, whole genome shotgun sequence.
ACCESSION   DAOQYE010000484 DAOQYE010000000
VERSION     DAOQYE010000484.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1778
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80163_length_1778_cov_2.528729"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1287
                     /locus_tag="PKN52_08085"
     CDS             <1..1287
                     /locus_tag="PKN52_08085"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HNQ99946.1"
                     /translation="PIRDNGQSEGAVNFMRLFDTLIDVTKQGATRRGSFAAYLPIEHP
                     DAREFLEIRGDGNPIQNLFFAVTVSDEWLESMVNGDRDKRELWARVLQQRSEVGLPYV
                     LFTGNADRGAADVYRDRGLPIRSSNLCSEISLPVSEDESFVCCLSSMNLLHFDEWADT
                     DAVETLVYFLDAVMSDFIEKSEGIPYLDRARRFAMRHRALGLGVLGWHSYLQSRLVSI
                     GSLTAAGLNRRVFKTVREAADGASAELARRYGEPELLEGYGRRNSTLMAIAPTTSSSF
                     ILGQVSQSIEPLKSNYYVRDLAKSVTTFRNPALAELLAEVDRDTPEVWRSILEHDGSV
                     QHLDFLSNEEKSVFATFSEVSQLDLVVQAGQRQEFIDQGQSLNLMVHPATPTKDLNAL
                     HLEAWRRGLKSLYYQHSMNAAQEFNRELLTCSVCEA"
     gene            1431..>1778
                     /locus_tag="PKN52_08090"
     CDS             1431..>1778
                     /locus_tag="PKN52_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HNQ99947.1"
                     /translation="MTPAGRRLTTPRLNLRPVEYPELLAYRDAIRHSYWLHTEYNLTD
                     DVHDFRARVSDVERSAIKNAMLAIAQVEVAVKTFWGDLYKRYPKPEVGAVGYTFAESE
                     VRHQDAYAHLLEIL"
BASE COUNT          305 a          661 c          546 g          266 t
ORIGIN      
        1 cccatccgcg acaacggcca gtcagagggg gcggtcaact tcatgcgcct cttcgacacc
       61 ctcatcgacg tgaccaagca gggcgccacg cgccgcggct ccttcgccgc ctacctcccc
      121 atcgagcacc cggacgcgcg cgagttcctc gagatccgcg gcgacggcaa ccccatccag
      181 aacctcttct tcgccgtgac ggtgagcgac gagtggctcg agagcatggt caacggcgac
      241 cgcgacaagc gcgagctgtg ggcgcgcgtg ttgcagcagc gctccgaggt cgggctgccg
      301 tacgtgctct tcacgggcaa cgccgatcgc ggcgcggccg acgtctaccg cgaccgcggc
      361 ctgcccatcc gctcgagtaa cctttgcagc gagatcagcc tgccggtgag cgaggacgag
      421 tcgttcgtct gctgcctctc gagcatgaac ctgctgcact tcgacgagtg ggccgacacc
      481 gacgccgtgg agacgctcgt ctacttcctc gacgccgtca tgagcgactt catcgagaag
      541 tccgagggca tcccctacct ggacagggcg cgccgcttcg ccatgcggca ccgcgcgctc
      601 ggcctcggcg tgctcggttg gcacagctac ctccagtcgc ggctcgtgtc catcggaagc
      661 ctgaccgccg ccggcctgaa ccgccgcgtg ttcaagaccg tccgcgaggc cgccgatggc
      721 gcctcggccg agctggcgcg ccgctacggc gagccagagc tgctcgaagg gtacggccgg
      781 cgcaactcca ccctcatggc catcgccccg accacgtcca gctcgttcat cctcgggcag
      841 gtatcgcagt cgatcgagcc actcaagtcg aactactacg tgcgcgacct cgccaagtcc
      901 gtcaccacgt tccggaaccc tgcgctcgcc gagttgctgg cggaggtaga ccgcgacacg
      961 ccggaagtct ggcgcagcat cctcgagcat gacggcagcg ttcagcacct cgacttcctc
     1021 tcgaacgagg agaagagcgt gttcgccacg ttctccgagg tcagtcagct cgacctcgtc
     1081 gtgcaggccg ggcagcgcca ggagttcatc gaccaggggc agtcgcttaa cctgatggtg
     1141 cacccggcca cgccgaccaa ggacctgaac gccctgcacc tcgaggcttg gcggcgcggg
     1201 ctcaagagcc tgtactacca acacagcatg aacgcggcgc aggagttcaa ccgcgaactg
     1261 ctcacctgct ccgtctgcga ggcgtgatgg caggcacttc cacgccaagc gtcgacgtgg
     1321 cgtcgaccag ccgttcagcg gcgtccgact ccgggtcctc cagctccgga gcgcccgcga
     1381 gcacgccagc gccggtcgac accgtcgaac cggccgggtc caccggcgcg gtgacccctg
     1441 ccggccggcg cctcacgacg ccgcgcctca accttcgccc tgtggagtac ccggaactcc
     1501 tcgcctaccg cgacgccatc cggcactcgt actggctcca cacggagtac aacctgacgg
     1561 acgacgtgca cgacttccgc gcgcgcgtca gcgacgtaga acgcagcgcc atcaagaacg
     1621 ccatgctcgc catcgctcag gtcgaggtgg ccgtcaagac gttctggggc gacctctaca
     1681 agcgctaccc caagcccgag gtcggagccg tcgggtacac gttcgccgag agcgaggtgc
     1741 gccaccagga cgcctacgcg cacctgctcg agatcctc
//
LOCUS       DAOQYE010000485         1776 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_80275_length_1776_cov_3.323068, whole genome shotgun sequence.
ACCESSION   DAOQYE010000485 DAOQYE010000000
VERSION     DAOQYE010000485.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1776
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80275_length_1776_cov_3.323068"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            96..980
                     /locus_tag="PKN52_08095"
     CDS             96..980
                     /locus_tag="PKN52_08095"
                     /inference="COORDINATES: protein motif:HMM:NF014658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis protein"
                     /protein_id="HNQ99948.1"
                     /translation="MSSRERAARPERVGIVSFTNVAPLNWGLKPWASGDGTAEFAYGV
                     PTKLNAALAAGDIDLTLVSSIEFIRHRDRYRALLDFSIATLGPVYSVMLFHRPAWRDL
                     QGVRIAVTTESATSVELLRVLLEADGVDAELVPTAGGLDDLLAAADAALLIGDAALKG
                     AVKHVPPTHGGPGGRPVSGRSAIDAGLHVTDLGEAWYRLTRLPFTFAVWASRDDARPS
                     EELVAAFREARRSGLGHLSAVAAVEAEKLGLPHAVVQRYLENFRYYLEPPDLDGLYAF
                     ARRSDPSFRDGELRFWEG"
     gene            1006..1203
                     /locus_tag="PKN52_08100"
     CDS             1006..1203
                     /locus_tag="PKN52_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99949.1"
                     /translation="MLDEMKDAEGAKPSLADFLAEVAKIQDFVRSLPDRDTGSPDELL
                     GYDEHGLWGATDSDVRDAGNE"
     gene            1308..>1776
                     /locus_tag="PKN52_08105"
     CDS             1308..>1776
                     /locus_tag="PKN52_08105"
                     /inference="COORDINATES: protein motif:HMM:NF021982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HNQ99950.1"
                     /translation="MQHAGLERTYYLHVPAGVEAREQPPVPLVLVLHGRGGDGPGTAD
                     LTDFDDVADERGFIVAYPTGVDGQWNYVDGIPGYELNVPDVEFLRALVAELSTRYPVD
                     PARVYVAGFSNGGYMAQHLACKATDLFAAFASVSAAGYGGQPTSCGDPDPVSIL"
BASE COUNT          265 a          637 c          595 g          279 t
ORIGIN      
        1 cctcgacccc tcgcttcgca cgccctctga cgccgcgctc gtgcgcacgg ccggccgcgt
       61 gcccgtcagg cgcgacgcgc tgtacaacga gctccatgtc ttcgcgtgag cgcgccgcga
      121 gacccgagcg cgtcggcatc gtctcgttca ccaacgtcgc gcccctcaac tggggtctga
      181 aaccctgggc gtcgggtgac ggcaccgccg agttcgccta cggtgtgccc accaagctca
      241 acgccgccct cgcggccggc gacatagacc tgacactcgt gtcgtcgatc gagttcatca
      301 ggcaccgtga ccgttaccgc gcgctgctcg acttctcgat cgccaccctc gggcccgtgt
      361 acagcgtgat gctcttccac cgaccggcgt ggcgcgacct gcaaggggtc cgcatcgccg
      421 tcaccaccga gtccgccacc agcgtggagc tcctcagggt gctgctggag gccgacggcg
      481 tcgatgccga gctggtgccg accgccggcg gcctcgacga cctcctggca gcggccgacg
      541 ccgcgctgct catcggcgac gcggcgctca agggcgccgt gaagcacgtc ccgcccacgc
      601 acggcggacc gggcgggcgg cccgtcagcg gccgcagcgc catcgacgca ggcctgcacg
      661 tgaccgacct gggcgaggcg tggtaccgcc tcacgcgatt gcccttcacc ttcgcggtct
      721 gggcgtcgcg ggacgacgcg cgcccctctg aggaactcgt cgccgccttc cgcgaggccc
      781 gccgctccgg gctgggacac ctgagcgccg tcgcggccgt ggaggcggag aagctcggcc
      841 tgccccatgc ggtcgtgcag cgctacctcg agaacttcag gtactacctc gagccgcccg
      901 acctggacgg tctgtacgcg ttcgcgcggc gcagcgaccc ctcgttcagg gacggggagc
      961 tgcggttctg ggaggggtga acagttgatt cgtagtggtg gcagaatgct ggacgagatg
     1021 aaagatgccg aaggagcgaa gccgagcctc gcggacttct tggcagaagt cgccaagatc
     1081 caggacttcg tcaggagctt gccggaccgg gataccggca gcccggacga gttgcttggt
     1141 tacgacgagc acggtttgtg gggcgcaacg gactcggacg tgcgtgatgc aggtaatgaa
     1201 tgagcagaaa gcgagttctg cgctttcgat gcggcgcgac cctattgctg cttgcccgcc
     1261 tgacgagcgc gtcacttgcc accgcgcaga cctccactaa cgaggccatg cagcatgccg
     1321 gcctcgagcg cacctactac ctgcatgtcc cggccggtgt cgaagcccgt gagcagcccc
     1381 cggtcccgct ggtcctcgtg ctccacggcc gcggtggcga tgggccgggc accgccgacc
     1441 tgaccgactt tgacgacgtc gccgacgagc gcggcttcat cgtggcttac ccgaccggcg
     1501 tggacggcca gtggaactac gtcgatggca tcccggggta cgagctgaac gtgccggacg
     1561 tcgagttcct gcgcgcacta gtcgcggagc tgtcgacccg ttacccggtc gatcccgccc
     1621 gggtctacgt ggccggcttc tcgaacggcg gctacatggc ccagcacctg gcctgcaagg
     1681 ccacggacct gttcgccgcc ttcgcgagcg tgagcgcggc cggctacggc gggcaaccga
     1741 ccagctgcgg cgaccccgac ccggtctcga tcctgt
//
LOCUS       DAOQYE010000486         1776 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_80299_length_1776_cov_2.596746, whole genome shotgun sequence.
ACCESSION   DAOQYE010000486 DAOQYE010000000
VERSION     DAOQYE010000486.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1776
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80299_length_1776_cov_2.596746"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..670)
                     /locus_tag="PKN52_08110"
     CDS             complement(<1..670)
                     /locus_tag="PKN52_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNQ99951.1"
                     /translation="MLDLVVGDLERFERRLADELRSDVAFIEAIGADLVTAGGKRLRP
                     AVAFLTGRLLRSESEAAMHVALAVELLHSASLLHDDLIDDADTRRGTEAAFRRYGNVV
                     SVMSGDFMLARVLRLLAATGSAAFTDLMAGTAAAICEAEVLQFQVATLQEYSWANYGR
                     IIDGKTAVLLAAAAQGVGLLAGTDEGGLAALGRFGMAYGRAFQLRDDYLDLLGDPAKL
                     GKPVG"
     gene            complement(723..1373)
                     /locus_tag="PKN52_08115"
     CDS             complement(723..1373)
                     /locus_tag="PKN52_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HNQ99952.1"
                     /translation="MQHDLPSVTVSRLVSYLRILADMEAEGVAYVTSERLGGASQVSA
                     HQVRKDLLLTRVTSGTRGRGYTVAILRRELSSTLGLTRSWRVAIVGMGRLGQALADYP
                     DFSGFNFSLLCGFDKDPAKVGRTVGSLPVYGTDELTARARELALDIAFLTVPVGSAQA
                     ATDLIAAAGIRGILNFVPTVLTVPESVRVEPVDFLAGLKRLAFYLQPHAAVAAAAG"
     gene            complement(1373..>1776)
                     /locus_tag="PKN52_08120"
     CDS             complement(1373..>1776)
                     /locus_tag="PKN52_08120"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNQ99953.1"
                     /translation="VADRGKAGNFGVTDATVYRYDPGGAGGTTAATTPATQTAQTTTA
                     PTTPQSDAAAAAPAQAAATSATAKYLQVGAYGSRQSALPQIERLQGLGFQVTERTEGT
                     LLKLLVGPYEGAALNEAKARLDAAGIESFAR"
BASE COUNT          269 a          585 c          661 g          261 t
ORIGIN      
        1 cgcccacggg cttcccgagc ttggccgggt cgccgaggag atcaaggtag tcgtcgcgga
       61 gctggaaagc gcggccgtag gccatgccga agcgtccgag cgctgcgagt ccgccctcgt
      121 cggtcccggc gaggagcccg acgccctgcg ccgccgcggc tagcaggacc gcggtcttgc
      181 cgtcgatgat gcggccgtag ttcgcccacg agtactcctg caacgtcgcc acctggaact
      241 gcaggacctc ggcctcgcag atggccgcgg cggtgccggc catcaggtcg gtgaaagcgg
      301 cgctgccggt ggcggccagg agccgcaaca ccctggcgag catgaaatcg ccagacatga
      361 cgctgacgac gttgccgtag cgccggaagg cggcttccgt gccgcggcgc gtgtcagcgt
      421 cgtctatcag gtcgtcgtgc agcagggagg ccgagtgcag gagctcgacc gcgagcgcca
      481 catgcatggc ggcctccgat tccgagcgga ggagcctgcc ggtgaggaag gcgaccgcgg
      541 gtctgagccg cttgccgccc gccgtcacga ggtcggcgcc gatggcttcg atgaaggcga
      601 cgtcggagcg caactcgtcg gccaggcgcc tttcgaaacg ttccaggtcg cctacgacga
      661 ggtccagcac ggggtcagta tatgcggccc tagtgccaaa cgtcacaagg gacgcttcgc
      721 ggtcagccgg ccgcggccgc caccgcggcg tgaggctgca ggtagaaggc gaggcgtttg
      781 aggccagcca ggaagtcgac gggctccacg cgcacgctct ccggcaccgt cagtaccgtg
      841 ggcacgaagt tgaggatgcc gcgtatgccg gccgcggcga tgaggtccgt cgcggcctgg
      901 gcgctcccga cgggaacggt gaggaacgcg atgtcgagcg ccagctcgcg ggcgcgcgcc
      961 gtgagctcgt ccgtgccgta cacggggagg gagccaacgg tcctgcccac cttggcgggg
     1021 tccttgtcga agccgcaaag gaggctgaag ttgaagccgc tgaagtccgg gtaatcggcc
     1081 agcgcctgcc cgaggcgacc catgccgacg atggcgacgc gccacgagcg ggtcagcccg
     1141 agcgtgctgc tgagctcccg tcgcaggatg gcgaccgtgt agccgcggcc ccgcgtgccg
     1201 gacgtgaccc tggtgagaag gaggtccttg cgcacctggt gggcggagac ctgcgaggcg
     1261 ccgcccaggc gctcggaagt gacgtaagcg acgccctcgg cctccatgtc ggcgaggatc
     1321 cgcaggtagc tgaccaagcg actgaccgtc acagacggca ggtcgtgttg catcaacgag
     1381 cgaagctctc gatgccggcc gcgtctaggc gcgccttggc ttcgttcagg gcggcacctt
     1441 cgtacgggcc gacgaggagc ttgaggaggg ttccctctgt gcgctccgtg acctggaagc
     1501 cgagcccttg caggcgctcg atctgtggca gagcgctctg acggctcccg taggctccta
     1561 cctgcaggta cttggccgta gcggacgtgg cggcggcctg cgcgggcgcg gcggcggccg
     1621 cgtccgactg cggggtggta ggggcggtcg tcgtctgcgc cgtctgagtg gctggcgtag
     1681 tcgccgccgt cgtaccaccc gcacccccgg ggtcgtatcg gtacaccgtg gcgtcggtga
     1741 cgccgaagtt gccagccttg ccacggtcgg cgaccg
//
LOCUS       DAOQYE010000487         1775 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_80355_length_1775_cov_2.871512, whole genome shotgun sequence.
ACCESSION   DAOQYE010000487 DAOQYE010000000
VERSION     DAOQYE010000487.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80355_length_1775_cov_2.871512"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..67)
                     /locus_tag="PKN52_08125"
     CDS             complement(<1..67)
                     /locus_tag="PKN52_08125"
                     /inference="COORDINATES: protein motif:HMM:NF012534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HNQ99954.1"
                     /translation="MATGKVKWFSSEKGFGFIEQGG"
     gene            complement(359..>1775)
                     /locus_tag="PKN52_08130"
     CDS             complement(359..>1775)
                     /locus_tag="PKN52_08130"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ99955.1"
                     /translation="SLGQALLTLGSVSAIMLQLGWAVVPLVLVASVPSVISSSRFGTE
                     GYRMLRRFAEDARRQNYLGSLLTSDQAVKEIRLFDFDDYLMERWQEYYLKFRAQLVSM
                     IRRRSGWGLATSLFSTGMVGTATYLVLRRAAAGLLTVGDFGMFIQGISQLQSQFGNLL
                     SGFSGIYQNLLYMRNLFEFLELPTSGAEAGEEWVGPISEIEFRDMSFRYPLTESDVLH
                     GVSFVVRKGETLALVGENGAGKSTIVKLMTALYRPSRGQILVNGQDSARFSAASLRAE
                     MSTVFQDFGQYQMTVRENIAIGKVARGMPRGGEAAPELDEAVRTAGERAGADGFISKL
                     PAGYDNMLGRWFEGGRQLSGGQWQRIALARLYYRGGSVLIFDEPTSALDADAEFEVIE
                     TLRAQARGRIAVVVSHRFSTVRLAERIIVLTAGHITERGTHEELMRLGGTYARMFTRQ
                     ARGYLDGYGDAVEGGEAGTGA"
BASE COUNT          302 a          614 c          575 g          284 t
ORIGIN      
        1 cgccgccttg ttcgatgaag ccgaagccct tctcgctgct gaaccacttg accttacctg
       61 ttgccatttt accttttccc taccgccgag cttggcttga cgccgagcac ggcacacgat
      121 ttccgggtcc gaggaccctg tcctgaaaca cccttcaggt tgtctcggaa cgccttgaat
      181 agtacgacag tgatcggctc atgacaagtg acgtctacgg catccgcctt gacgacaccg
      241 gttagtgaag gaacggcctg ctcctgctca tgcgacacgc tcagaccgta ggcgtgagcg
      301 acacgctcag gccaatggcg tgagcgacac gctcaggcca atggcgtgag cgacacgctc
      361 aggcgcccgt gccggcctcg cccccctcga ccgcgtcgcc gtaaccgtcc aggtagcccc
      421 gcgcctgccg cgtgaacatg cgcgcgtagg tgccgcccaa ccgcatgagc tcctcgtgcg
      481 tgccgcgctc cgtgatatga ccggcggtca ggacgatgat gcgctccgcg agcctgacgg
      541 tgctgaagcg gtgcgatacg acgaccgcga tgcggccgcg tgcctgagcg cgcagcgtct
      601 cgatgacctc gaactccgcg tccgcgtcga gggcgctggt tggctcgtcg aagatcagca
      661 ccgacccacc gcggtagtag agccgcgcca gggcgatgcg ctgccactgg cctccggaaa
      721 gctgcctccc gccctcgaac caacggccga gcatgttgtc gtagccggcg gggagtttcg
      781 agatgaagcc gtcggctccg gcccgctccc ccgcggtgcg cacggcctcg tcgagctccg
      841 gcgcggcctc gccgccccgc ggcatgccgc gcgccacctt gccgatagcg atgttctcgc
      901 gcacggtcat ctggtactgc ccgaagtcct ggaacaccgt gctcatctcg gcccgcaggc
      961 tggccgcgct gaaacgcgcc gagtcctggc cgttcaccag gatctgcccg cgcgacgggc
     1021 ggtagagggc ggtcatgagc ttcacgatgg tcgacttgcc cgcgccgttc tcgccgacga
     1081 gcgccagcgt ctcgcccttg cgcaccacga aggacacgcc gtgaagcacg tcgctctccg
     1141 tgagcgggta gcggaagctc atgtcgcgga actcgatctc ggagatcgga ccgacccact
     1201 cctcgcccgc ctcggcgccg ctcgtcggga gctcgaggaa ctcgaagagg ttgcgcatgt
     1261 agagcaggtt ctggtagatg ccgctgaagc cggagaggag gttcccaaac tggctctgca
     1321 gctgcgagat gccctggatg aacatgccga agtcgccgac cgtgaggagg ccggccgcgg
     1381 cgcgcctcag cacgaggtag gtggccgtgc ccaccatgcc ggtggagaag agcgacgtcg
     1441 ccaaccccca gccgctccgc cgcctgatca tggagacgag ctgggcgcgg aacttgaggt
     1501 agtactcctg ccaacgctcc atgaggtagt cgtcgaagtc gaagagcctg atctccttga
     1561 cggcctggtc gctcgtgagg agcgagccga ggtagttctg gcgccgcgcg tcctccgcga
     1621 accgcctgag catgcggtac ccctccgtgc cgaagcgcga ggaggagatg acgctcggca
     1681 cggaggcgac gagcaccagc ggcacgaccg cccagccgag ctgcaacatg atggcgctga
     1741 ccgagccgag ggtcagcaac gcctgcccga gcgag
//
LOCUS       DAOQYE010000488         1775 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_80396_length_1775_cov_1.762791, whole genome shotgun sequence.
ACCESSION   DAOQYE010000488 DAOQYE010000000
VERSION     DAOQYE010000488.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80396_length_1775_cov_1.762791"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..571)
                     /locus_tag="PKN52_08135"
     CDS             complement(<1..571)
                     /locus_tag="PKN52_08135"
                     /inference="COORDINATES: protein motif:HMM:NF015147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HNQ99956.1"
                     /translation="MKLRLAAMLLAALVGATLLAACAPGAAVLAPPTFKLDAANSGFV
                     RIDPPGVGDGSALFRLALSVENPNPVGVRLAGLDGDLFLRDARAASSSFRGGIDLPAR
                     ASAPLVLDVKVPLGAAPVLLDTIANFVAGNAVQYRFDAAVTIDVLGAPQRFPTFTVAR
                     GELTRPAGLTPPQFALTGTELRFEALDRAR"
     gene            671..1486
                     /locus_tag="PKN52_08140"
     CDS             671..1486
                     /locus_tag="PKN52_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNQ99957.1"
                     /translation="MSTGTPSDAVRGEARTVSRAVRGHVAVLTLDRPERRNALSRALR
                     ADLTDALAWAEAEPAVRAVVLTGAGKAFCAGLDLAELEGTLTYAEAEHQRDSEALARL
                     YLLLLTLSKPVIAAVNGPAVAGGAGLVTACDLAVMGRGATLGYTEARIGFVAALVGVL
                     LARQVGEKHVRDLLLSARLVGAEEAQRMGLVNAVVADDEVLESACARAAELARNAPGS
                     LALTKRLLLEAPGLPLEEGLARAVAINVSARSGAELAEGVRAFLERREPAWRA"
BASE COUNT          242 a          612 c          688 g          233 t
ORIGIN      
        1 gccgcgcgcg gtcgagcgcc tcgaaccgca gctcggtgcc cgtgagcgcg aactgcggcg
       61 gtgtcagacc ggcgggcctc gtcaactcgc cgcgcgccac cgtgaaggtc gggaaacgct
      121 gtggggcgcc gaggacgtcg atggtgacgg ccgcgtcgaa gcggtactgg acggcgttgc
      181 cggccacgaa gttggcgatg gtgtcgagga ggacgggcgc ggcgccgagc ggcaccttaa
      241 cgtccagtac gaggggggcg ctggcgcggg ccggcagatc gatgccgccg cggaaggacg
      301 aggaggccgc gcgcgcgtcc cggaggaaca ggtcgccgtc gaggccggcg aggcgcacgc
      361 cgacggggtt ggggttctcg acgctgagcg ctaggcggaa gagcgcggag ccgtcgccga
      421 caccgggcgg gtcgatgcgg acgaagccgg agttggcggc gtccagtttg aacgtcggcg
      481 gcgccaggac ggccgcgccc ggggcgcagg ccgcgagcag cgtggcgccg accaaggcgg
      541 cgaggagcat ggcagcgaga cggagcttca ccctcaaagc gtactagcct cgtatgaggg
      601 gcgcagtcgg tttgttcgca ggcccaggcc cgttcgtgag ccgcgcgcca ccctaccggt
      661 atcatcgggc atgtcgaccg gtacgccttc cgatgcggtc cgaggcgagg cgcgcaccgt
      721 ctcccgcgcc gtgcggggcc acgtcgcggt tctgacgctg gatcggcccg agcgacggaa
      781 cgcgctctcg cgcgcgttgc gggccgacct gaccgacgcc ctggcctggg ccgaagcgga
      841 gcccgccgtg cgcgccgtcg tgctgaccgg ggccgggaag gcgttctgcg cggggctgga
      901 cctggccgag cttgaaggca ccctgacgta tgccgaggcg gagcaccaaa gggactcgga
      961 ggcgttggcc cgactctact tgctgctcct caccctctcc aagccggtca tagcggccgt
     1021 gaacggcccc gccgtggcag gcggcgccgg gctcgtgacc gcgtgcgatc tcgccgtcat
     1081 gggacgcggc gccactctcg gctacacgga ggcccgcatc ggcttcgtcg ccgcgctcgt
     1141 cggcgtgctg ctcgcgcgcc aggtgggcga gaaacacgtg cgcgacctgc tcctgagcgc
     1201 gcgcctcgtc ggagcggaag aagcgcagcg catgggcctc gtcaacgccg tggtcgccga
     1261 tgacgaggtc ctcgagtcgg cgtgcgcaag ggcggccgaa ctggcgcgca acgcccccgg
     1321 ctcgctggcc ctcaccaagc ggctgctcct cgaggcgccg gggctgccgc tcgaagaagg
     1381 cctggcgcgg gccgtcgcca tcaacgtgag cgccaggtcg ggcgcggagc tcgccgaagg
     1441 ggtgcgggcg ttcctcgagc ggcgggagcc cgcgtggcgg gcctgacgga gcgggacggc
     1501 tagcggccga gcctggccaa gtcggcctat ccgacgcatg gccggtccgg ctgatcgtcg
     1561 agccgccggt cggcaagccc gcgtgccgcc gagtcttgcc aggcgtcggc cagcgcagta
     1621 ctatgctact catgtgtaac ttacacacgc gctggatggc ctgggcatga cagtcgctac
     1681 gcgcgtcctg ctcctctgcg gtgggcgctc cgcggagcac gaggtctccc tcgcgtccgc
     1741 ccgcgccgtc gctgcggccg ccaccgcggc cgcca
//
LOCUS       DAOQYE010000489         1773 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_80496_length_1773_cov_2.810244, whole genome shotgun sequence.
ACCESSION   DAOQYE010000489 DAOQYE010000000
VERSION     DAOQYE010000489.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80496_length_1773_cov_2.810244"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1057)
                     /gene="glyA"
                     /locus_tag="PKN52_08145"
     CDS             complement(<1..1057)
                     /gene="glyA"
                     /locus_tag="PKN52_08145"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HNQ99958.1"
                     /translation="MTRNAYVPVRDERTFELIGAEYARQAGGLELIASENFVSRQVME
                     AVGSVLTNKYAEGYPGKRYYGGCEVVDQVEQLAIDRAKELFGAAWANVQPHSGSNANF
                     AAYYALLESGDKVLGMDLAHGGHLTHGSPVNFSGRSYEVVGYPVDRRTERIDYDALRR
                     LAHEHRPKMIIAGASAYSRFIDFAAFRAVADEVGAILMADIAHIAGPIAAGLHPHPLP
                     YAHVVTTTTHKTLRGPRGGLIFGNDEEIAKKIDRVIFPGIQGGPLEHVVAGKAVAFFE
                     ALQPSFRDYQALILANAIELAARLGERGYRIVSGGTDNHLFVVDLQGTGINGNTASKR
                     LDKVGITVSKSMVPFDPE"
     gene            1275..>1773
                     /locus_tag="PKN52_08150"
     CDS             1275..>1773
                     /locus_tag="PKN52_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNQ99959.1"
                     /translation="MSGPTRSGLLAGKVAVVTGASSGIGKAIAEDYAREGASVVLADI
                     NDAAGEAAAAAINAAGGVASYCHSDAADPQAADALVRFAQERYGALHVACNNAGISGE
                     SNPVADHSVAGWHKVIDVNLNSVFYGMRAQIPAILAAGGGAIVNVASILGQVGFAGAP
                     AYVAAK"
BASE COUNT          263 a          594 c          635 g          281 t
ORIGIN      
        1 tctcgggatc gaacggcacc atgctcttgc tgacggtgat cccgaccttg tcaagacgct
       61 tgctggcggt gttgccgttg atgcccgtgc cttgcagatc gaccacgaag aggtggttgt
      121 cggtgccgcc cgacacgatg cggtagccgc gctcgccgag gcgcgccgcc agctcgatgg
      181 cgttggcgag gatcagcgcc tggtagtcgc ggaacgaggg ctggagcgcc tcgaagaacg
      241 ctacggcctt gccggccacg acgtgctcga ggggaccgcc ctggatgcca gggaagatga
      301 cgcggtcgat cttcttggcg atctcctcgt cgttgccgaa gatgaggccg ccccgcgggc
      361 cgcgtagcgt cttgtgggtc gtggtcgtca cgacgtgcgc gtagggcagc gggtgcggat
      421 ggagtccggc cgctatcggc cccgcgatgt gcgcgatgtc ggccatgagg atggcgccga
      481 cctcgtccgc caccgccctg aacgcggcga agtcaatgaa gcggctgtac gcgctggcgc
      541 ccgcgatgat catcttcggg cggtgctcgt gcgcgaggcg cctcagggcg tcgtagtcga
      601 tccgctccgt gcgacggtcg accgggtagc ccacgacctc gtacgagcgg ccggagaagt
      661 tcaccggcga cccgtgcgtc aggtggccgc cgtgggccag gtccatgccg aggaccttgt
      721 cacccgactc gagcagggcg tagtaggcgg cgaagttggc gttggagccg gagtgaggct
      781 ggacgttcgc ccaggcggca ccgaacagct ccttggcgcg gtcgatggcg agttgctcga
      841 cttggtcgac gacctcgcag cccccgtagt accgcttgcc cgggtaccct tccgcgtact
      901 tgttggtgag gacggagccg acggcctcca tcacctgcct ggagacgaag ttctcggagg
      961 cgatgagttc gaggccgcca gcctggcggg cgtactctgc gccgatgagc tcgaaggtcc
     1021 gctcgtcacg gacgggaacg tacgcgtttc gcgtcatggc cgcaagtata gcttccatgc
     1081 tccgcgaccc ggcgcccccg gcgcggagcc gcgtgaaccg accggtgccg acccatgcgc
     1141 gcctggcgcg ttctggtcgc gccacctgaa cccggcccgc cgggtctccc gtcccggcgg
     1201 gccggctggg cttgtgaaat cctgaactcg acgggacacc cccttcgggt gcgtccgccg
     1261 atcggagggc gaacatgagc ggtcccacga gatccggact tctggcaggc aaggtagcgg
     1321 tggtgacggg cgcgagttcc ggcatcggca aggccatcgc ggaggattac gcgcgcgagg
     1381 gcgcctccgt cgtcctggcg gacataaacg acgcggcagg cgaggccgcg gcggcggcca
     1441 tcaacgccgc gggcggcgtg gcgagctact gccactccga cgccgccgac ccgcaggcgg
     1501 ccgacgcgct cgtgcggttc gctcaagagc gctacggcgc gctgcacgtg gcttgcaaca
     1561 acgcgggcat ctccggagaa tcgaaccccg tggccgacca ctcggtggcc ggctggcaca
     1621 aggtcatcga cgtcaacctc aacagcgtct tctacggcat gcgcgcgcag atccccgcca
     1681 tcctggccgc cggcggcggc gccatcgtga acgtggcatc gatcctcggg caggtcggct
     1741 tcgcgggcgc cccggcgtac gtggccgcca aac
//
LOCUS       DAOQYE010000490         1773 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_80511_length_1773_cov_2.490687, whole genome shotgun sequence.
ACCESSION   DAOQYE010000490 DAOQYE010000000
VERSION     DAOQYE010000490.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80511_length_1773_cov_2.490687"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(322..>1363)
                     /locus_tag="PKN52_08155"
     CDS             complement(322..>1363)
                     /locus_tag="PKN52_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HNQ99960.1"
                     /translation="DITELADKASYEEVVYLLWNGSLPNRQQLESFKAEIAPHYAVPD
                     AVYDLYRSLPHGHSPMHAIRTAASMLGAYDEDPDGVDLPNVRRIGLKLLAQFQTITAA
                     YERIRRGLEPVAPRPDLSLAGNFLYALTGETPSEAAARVMDVALVLHAEHGSNASTFV
                     ARATASTLTDVYSAITAAIGALKGPLHGGANTAVMQALEGIGDVAGVEPYVLDVLSKP
                     GGRVMGFGHRVYKVLDPRAQVLKEVSRKLAQESGDSKWFDMSLEMERVMDREMAKKGK
                     QVKPNVDFFSASVYRMLGFPSDMYTPIFAVARISGWMAHLFEQYADNRLMRPRLAYSG
                     EMGLEFVPLDRR"
     assembly_gap    1364..1373
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          250 a          543 c          656 g          314 t
ORIGIN      
        1 ggctcttgag tcgtcgagga gcgcgtaccc gtggagcatc ctgtcgagtt cggtcgttgc
       61 gtgggggagg ttggtggttc gtcctcgatg gtgtctcagt cgtgcgtgac gtgtgacgcc
      121 gtgccgtcaa gagtgaacag accctaagcc gagcgacgct cgactgagtg ggccggcgcg
      181 tgactcagcc agcgccatac gagctcggct gctggctgaa cccggctggt ggtcctgagg
      241 gaggcgagcg gccggatcgg ctggcaaagc ccggtccggc cgcgttgatc tggacggaag
      301 ctgatctgga cggaagctag ctcagcgccg gtcgagcggg acgaactcga gccccatctc
      361 gccggagtac gcgaggcgcg gccgcatgag ccggttgtcg gcgtactgct cgaacaggtg
      421 cgccatccag ccgctgatac gggcgacggc gaagataggc gtgtacatgt cggaagggaa
      481 gccgagcatg cggtagacgg aggctgagaa gaagtcaacg ttcggcttga cctgcttgcc
      541 cttcttggcc atctctcggt ccatcacgcg ctccatctcg aggctcatgt cgaaccactt
      601 cgagtcgccc gactcctggg cgagcttgcg gctcacttcc ttcagcacct gggcgcgcgg
      661 gtcgaggacc ttgtatacgc gatgcccgaa gcccatgacg cgcccgcccg gcttcgagag
      721 cacgtccagc acgtacggtt cgacgccggc cacatcgccg atcccttcca gcgcctgcat
      781 gacggccgtg ttggcgccgc catgcagcgg gcccttgagg gcgccgatgg cggccgtgat
      841 ggcgctgtag acgtcggtga gtgtcgaggc ggtggcgcgc gctacgaacg tgctcgcgtt
      901 ggagccgtgc tcagcgtgca gcacgagcgc cacgtccatg acgcgcgcgg cggcttcgga
      961 tggggtctcc ccggtgagcg cgtacaggaa gttgccggcc aggctcaggt cgggtcgcgg
     1021 agcgaccggt tcgaggccgc gccggatgcg ttcgtatgcc gccgtgatgg tctggaactg
     1081 cgcgaggagc ttgaggccga tgcggcggac gttgggcagg tcgacgccgt cggggtcctc
     1141 gtcgtaggcg cccagcatgg acgcggccgt gcggatggcg tgcatgggcg agtggccgtg
     1201 cggcaggctg cggtagaggt cgtagacggc gtccggcacg gcgtagtgcg gcgcgatctc
     1261 ggccttgaag ctctcgagct gctgccggtt gggcaggctg ccgttccaga gcaggtacac
     1321 gacctcttcg tagctggcct tgtcggcgag ctccgtgatg tcgnnnnnnn nnnccgctgt
     1381 agacgagctg gcccttgccg ccgtcgatga aagagatggc gctagtgtcg atgtagacgc
     1441 catctaggcc tcggttgatc gtctgctcca tgaatcctcc gcgtgtccac cgcgaggtgt
     1501 gagttcgcgg ttcgcttccc aaggggtgcg cggtggcgtg acggcgctgg cgcgtaagtg
     1561 ggtgctgcct tcggcttccc cgggatcgcc caactccgtg gtcgcgggcg gggtcgggcc
     1621 catgcacctc gtgatcgccc cacgcgcggc tcgaccgcta ccgcggtctc aagtcggggt
     1681 ccagtgtacc cggcggggac cggcagcgcg cgccgcggtg ggcagaaacg ggcgaacgtc
     1741 gcgcggccgg tctcgggcgg gtgactcgcc ttc
//
LOCUS       DAOQYE010000491         1773 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_80516_length_1773_cov_2.345751, whole genome shotgun sequence.
ACCESSION   DAOQYE010000491 DAOQYE010000000
VERSION     DAOQYE010000491.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80516_length_1773_cov_2.345751"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..745
                     /gene="lnt"
                     /locus_tag="PKN52_08160"
     CDS             <1..745
                     /gene="lnt"
                     /locus_tag="PKN52_08160"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HNQ99961.1"
                     /translation="IGYDLTAGDAGTLRSAITASAPGATFVVGGRVYDGDGSYNSLIA
                     LRADAELGHYDKHYLVPFGERWPLIETLSGLYAAVFRAFGLPMLAGTTPGEVIEPIAS
                     PLGSLGAFVCYESVFPQVQRLLVERGAGVLVLGTNDAWFGVGTGARQHFDMGRLRAIE
                     TRRWLLRAGNDGVTAAVDPWGRVTAEVERGVATTLTVSFDTRDDLTLWTRHGAWTPVA
                     LLAWAALVAAVGLISAGRSGSPVDPAGQA"
     gene            817..>1773
                     /gene="trkA"
                     /locus_tag="PKN52_08165"
     CDS             817..>1773
                     /gene="trkA"
                     /locus_tag="PKN52_08165"
                     /inference="COORDINATES: protein motif:HMM:NF007039.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HNQ99962.1"
                     /translation="MQVVIVGGGEIGALLAAALHRTHGVIVIDQDPEREAAFASLDVQ
                     FVRGNGTDPDDLRTAQVERARAFIACTASDDVNVLACLAAKGLGAKLTLAFVTRQRYV
                     DAFKRDGAFESVGLLIDRVLWPQRILAHQIADIVRAPRAVDSARFANGRITLLEFLLE
                     PHDPFRGENLSSLALPQGVLAVGAIKGEDFIIPTGATVFEAGDRVVFMGTTERMRELQ
                     RRFAPHKRSPRVVIVGGGNVGFMVAQQLMTDRANITIIEEDPERCEKLAQLLPHALVL
                     KGDGTDLELLEQERAEDADVMVAVTDDDAKNLLVSLLSKQLGI"
BASE COUNT          265 a          606 c          611 g          291 t
ORIGIN      
        1 catcggctac gacctgacgg ccggcgacgc gggcacccta cggagcgcga taacagcctc
       61 ggcaccaggt gccacgttcg tggtgggcgg gcgcgtgtac gacggggacg gcagctacaa
      121 ctcgctcatc gcgctgcgcg cggacgcgga gctcggacat tacgacaagc actacctggt
      181 gcccttcggc gagcgctggc cgctcatcga gacgttgagc ggcctctatg cggccgtgtt
      241 cagggcgttc gggctgccga tgctggcggg cacgacgccg ggcgaggtca tcgagcccat
      301 cgcgtccccc cttggatcac tcggcgcctt cgtctgctac gagtccgtgt tcccgcaagt
      361 gcagcgcctg ctcgtcgagc ggggcgccgg cgtgctggtc ctcggcacga acgacgcgtg
      421 gttcggggtc ggcaccggcg cgcgccaaca cttcgacatg ggccgcctgc gcgccatcga
      481 gacgcggcgc tggctgctcc gcgccggcaa cgatggcgtt acggccgccg tcgacccgtg
      541 ggggcgtgtg acggccgagg tcgagcgcgg cgtggcgacg accctgaccg tctcgttcga
      601 tacgcgcgac gacctcacgc tctggacgcg ccacggcgcc tggacgccgg tcgcgctgct
      661 cgcgtgggcg gcgctcgtag ctgcggtggg gctgatctct gccggacgat ccggttcacc
      721 agtcgaccct gccgggcagg cgtgaggctt cacacctcgc ctgggcgaga ccgattccgt
      781 tgcgcccgta gcgctgacct gtagtatgag cgacctatgc aagtagtcat cgtcggtggc
      841 ggcgagatcg gggctctgct cgcggccgct ctgcaccgta cccacggtgt catcgtgatc
      901 gaccaagatc ccgagcgcga ggcagcgttc gcctccctgg acgtgcagtt cgtccgcggc
      961 aacggcaccg acccggacga cctgcgcacc gcccaagtgg aacgcgctag ggctttcatc
     1021 gcctgcacgg cgtctgacga cgtgaacgtc ctggcctgcc tggcggccaa gggtctcggc
     1081 gcgaagctga cgctcgcttt cgtcacccgc cagcgttacg tcgacgcgtt caagcgcgac
     1141 ggcgccttcg agtcggtcgg gttgttgatc gaccgagtac tgtggcccca gcggatcctc
     1201 gcgcaccaga tcgccgacat cgtgcgcgcc cctcgggcag tagacagcgc gcgtttcgcc
     1261 aacgggcgga tcaccctcct cgagttcctc ctcgagcccc acgacccgtt ccgtggcgag
     1321 aacctctctt ccctggctct tccgcagggc gtgctcgccg taggtgccat caagggcgag
     1381 gacttcatca tcccgaccgg cgcgacggtg ttcgaggccg gcgaccgggt cgtgttcatg
     1441 gggacgaccg agcgcatgcg agagttgcag cggcgcttcg ctccgcacaa gcgctcgccg
     1501 agggtggtga tcgtgggcgg cggcaacgtc gggttcatgg tcgctcaaca gctgatgacg
     1561 gacagggcca acatcacgat catcgaggaa gacccggagc gttgcgagaa gctcgcgcaa
     1621 ctgctgccgc atgcgctcgt gctcaagggc gacggcacgg acctggaact cctggagcag
     1681 gagcgtgcgg aggacgcgga cgttatggtc gccgtcacgg acgacgacgc caagaacctg
     1741 ctcgtgtcgc tcctcagcaa gcagctcggc atc
//
LOCUS       DAOQYE010000492         1762 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_81325_length_1762_cov_3.130053, whole genome shotgun sequence.
ACCESSION   DAOQYE010000492 DAOQYE010000000
VERSION     DAOQYE010000492.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81325_length_1762_cov_3.130053"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..909
                     /locus_tag="PKN52_08170"
     CDS             <1..909
                     /locus_tag="PKN52_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99963.1"
                     /translation="AAARLVQHARAAAALQGLRGSVVGLLGYRAPGFYPATFDELLLR
                     RTFGVRVEHLGIQEVTDRVKAGAFREPPVEVFPTIEGGELGPEAVRWLGRYYGALFEA
                     VAAGGLGAVAIKDWPEMAPFVPEIPAGIWPALSWLQDDGVNVAPEGDVNGAVTMELLA
                     RLTGGGMPFFADISAFDASASTLVLWHYGGGTKLARSPSEIRFGADGRELEFSLKPGA
                     GVLARLGYSRGAYRLLLIEVEVLDERLTLRRAAGRVRTTRTNAAAVVEELLSGGWEHH
                     VSFVHGEVAEALGAFAKQAGLPITRL"
     gene            919..>1372
                     /locus_tag="PKN52_08175"
     CDS             919..>1372
                     /locus_tag="PKN52_08175"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNQ99964.1"
                     /translation="MIEGARYAHTNLVARDWRALAAFYTDLFGCRFVPPERDDAGALL
                     EAGTGVAGARMAGVHLRLPGHGDMGPTLEIYAYFPLLEAPGTAVNRPGYGHLAFEVAS
                     VVDARERVLAAGGRSVGEVVTMERKDGSKVTWCYVTDPEGNIVELQAWS"
     assembly_gap    1373..1382
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1427..>1762
                     /locus_tag="PKN52_08180"
     CDS             1427..>1762
                     /locus_tag="PKN52_08180"
                     /inference="COORDINATES: protein motif:HMM:TIGR02293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99965.1"
                     /translation="MAGAMFVPTRSAPPVLGVRALALEADSEFEVAARIKEGLPSSAV
                     HALARQLDRPSKYVLEVAGIPESTFHSHQKTRKPLSADASSRVYQVARVVEAAEEYFG
                     GDKDAARRWL"
BASE COUNT          248 a          567 c          656 g          281 t
ORIGIN      
        1 gccgccgcgc gtctcgtgca gcacgcccgc gcggccgccg cgctgcaggg cctgcgcggc
       61 agcgtcgtcg gtctcctggg ctaccgcgcg cccggcttct atccggccac gttcgacgag
      121 ctgctgctgc ggcgcacgtt cggcgtgcga gtggagcact tgggcatcca ggaagtcacg
      181 gaccgcgtga aggcgggtgc tttcagggag ccgcccgtcg aggtgttccc caccatcgag
      241 gggggagagc taggacccga ggccgtgcgg tggttggggc ggtactacgg cgcactcttc
      301 gaggccgttg cggccggtgg gctcggcgcg gtggccatca aggactggcc cgagatggcg
      361 ccgttcgtcc ccgagatccc ggccggcatc tggccggcgc tcagctggct gcaggacgac
      421 ggcgtgaacg tggcgcccga aggcgacgtc aacggcgccg tgacgatgga gctgctcgcg
      481 cgcctgacag gcggcggcat gccgttcttc gcggacatca gcgcgttcga cgcgagcgct
      541 tccacgctcg tcctctggca ttacgggggc ggtaccaagc tcgcgcgctc cccgagcgag
      601 atcaggttcg gcgcagatgg gcgcgagctc gagttctcgc tcaaaccggg ggcgggagtc
      661 ctggcgcgcc taggctacag ccgcggggct taccggttgc tgctcatcga ggtggaagtc
      721 ctggatgagc gcttgaccct gcggcgggcc gcgggccgcg tgcggaccac gcgcacgaac
      781 gccgccgccg tggtcgagga actgttgagc ggaggctggg agcaccacgt gtcgttcgtg
      841 catggcgagg ttgccgaggc gctcggtgcg ttcgcgaagc aggcggggtt gccgatcacg
      901 cggctgtaat ctggccgcat gatcgaaggt gcccgctacg cgcacacgaa cctcgtcgcc
      961 cgcgactggc gggccttggc cgcgttctac accgacctct tcggctgccg gttcgtgccg
     1021 cccgagcgcg acgacgccgg cgctctcctc gaggccggta cgggcgtggc gggcgccagg
     1081 atggccgggg tgcacctgcg cctgcccgga cacggcgaca tggggccgac gctcgagatc
     1141 tacgcgtact tcccactgtt ggaggcgccc ggcacggccg tcaaccgccc cggctacggg
     1201 cacctcgctt ttgaggtcgc gtccgtggtg gacgcgcggg agcgcgtcct ggccgccggt
     1261 ggtcgcagcg tgggcgaggt cgtcacgatg gagcgcaagg acggctcgaa ggtcacgtgg
     1321 tgttacgtga ccgatccaga agggaacatc gtcgaactgc aggcttggag ttnnnnnnnn
     1381 nnactccata tctggggtag actgaaccca tggatggagg cgtgtgatgg ccggcgccat
     1441 gttcgttcca acccgctccg cgccgcccgt tctgggcgtg cgtgctctgg ccctcgaagc
     1501 ggatagtgag ttcgaggtcg cggcccgcat caaggaaggg cttccgagtt ccgccgtcca
     1561 cgccttggcc aggcagctcg accgcccgag caagtacgtc ctcgaggtcg ccgggatccc
     1621 agagagcacc ttccatagcc atcagaagac gcgcaagccg ttgtcggcgg acgcgtcgag
     1681 cagggtgtac caggtcgcga gagtggtaga ggcggcggag gagtacttcg gtggcgacaa
     1741 ggatgcggcg cggcgctggc tc
//
LOCUS       DAOQYE010000493         1759 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_81535_length_1759_cov_3.029343, whole genome shotgun sequence.
ACCESSION   DAOQYE010000493 DAOQYE010000000
VERSION     DAOQYE010000493.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1759
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81535_length_1759_cov_3.029343"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="PKN52_08185"
     CDS             <1..352
                     /locus_tag="PKN52_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99966.1"
                     /translation="IQPLWFRKVRQSPWALFFIAIIVSVGMWLERFVIFVVSLTKDFL
                     ISSWADYVSTVWDWSLFIGSLGLFSTLFFLFIRLLPSIATAETKETAYHDHHHGSDAF
                     EHARMEYFHAEEPA"
     gene            349..912
                     /locus_tag="PKN52_08190"
     CDS             349..912
                     /locus_tag="PKN52_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HNQ99967.1"
                     /translation="MSSATLAHSASASNLYGLVAQFDSVEAIREASTRVRDAGYTKID
                     AYTPFPVEGLDMDLGMKPTRLGWVVLIMGITGGLGGFFMQWYANTSHYPLVSGGKPLN
                     SWPNWIVIMYECTILFAGLTAAITMLVRNGLPRPYHPIFNTPGFDNAMRDRFFLCIEA
                     RDPKFDLHSTRAFLEGLSPLIISEVQK"
     gene            909..1583
                     /locus_tag="PKN52_08195"
     CDS             909..1583
                     /locus_tag="PKN52_08195"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNQ99968.1"
                     /translation="MTSQSLAASDASRAHAGARRATRGANRRVRVKRGLVAAVVLGTL
                     LLTGCGRNMYDQPKAKAFESSPFFADGSAMRPLPTGTVSREFGALDPAYLTGMGPTGF
                     LSDLPIELTADLLERGRERFNIFCSPCHNFNADGLGATVLKGFPQPTDFTTTQRLLDA
                     PVGYFYNAMTNGFGRMYSYASRIPVADRWAIAAYVKALQLSQNAGVVDIPAGVTLAPS
                     ATEANR"
     gene            1580..>1759
                     /locus_tag="PKN52_08200"
     CDS             1580..>1759
                     /locus_tag="PKN52_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99969.1"
                     /translation="MSSLKLPPINTSKAQVGGLVVGAVALLIAVVLGFAGQAEGFFQS
                     YLFTYLFWLGLSLGSL"
BASE COUNT          268 a          654 c          521 g          316 t
ORIGIN      
        1 catccaaccg ctctggttcc gcaaggtaag gcagagcccg tgggcgctct tcttcatcgc
       61 catcatcgtg agcgtcggca tgtggctcga gcgcttcgtg atcttcgtgg tgtcgctcac
      121 gaaggacttc ctcatctcct cctgggccga ctacgtctcg acggtctggg actggtcgct
      181 cttcatcggc agcctcggcc tcttctcgac cctcttcttc ctcttcatcc ggctgctgcc
      241 ctccatcgcg acggccgaga cgaaggagac ggcgtaccac gaccaccatc acggcagcga
      301 cgccttcgag cacgcccgta tggagtactt ccacgctgag gagccggcat gagcagcgcc
      361 accctcgccc actccgcctc cgcgtcgaac ctgtacgggc tagtcgccca gttcgacagc
      421 gtcgaggcca tcagggaagc ctcgacccgt gtccgcgacg ccggctacac gaagatcgac
      481 gcctacacgc ccttccccgt cgaagggctc gacatggacc tcggcatgaa gccaacgcgg
      541 ctcggctggg tggtgctgat catgggcatc acgggcgggc tcggcgggtt cttcatgcag
      601 tggtacgcga acacgtccca ctacccgctc gtctccggcg gcaagccgct caacagctgg
      661 ccgaactgga tcgtcatcat gtacgagtgc acgatcctct tcgccggcct gacggcggcc
      721 atcaccatgc tggtgcgcaa cggcctgccg cggccgtacc acccgatctt caacaccccg
      781 gggttcgaca acgccatgcg cgaccgcttc ttcctctgca tcgaggcgcg cgacccgaag
      841 ttcgacctgc actccacccg cgccttcctc gaggggctct cgcccctcat catctcggag
      901 gtgcagaagt gacctcgcag tcgctcgccg cctccgatgc gtcccgtgcg cacgccggcg
      961 ccaggcgcgc caccaggggc gccaacaggc gcgtccgcgt gaagcgtggc ctggtggccg
     1021 cggtcgtgct cggcacgctg cttcttacgg gttgcggccg caacatgtac gaccagccca
     1081 aggccaaggc cttcgaatcg tcccccttct tcgccgacgg ctcggctatg cgccccttgc
     1141 cgacgggcac ggtctcacgt gagttcggcg ccctcgaccc ggcgtacctg accgggatgg
     1201 ggccgacggg cttcctctcc gacctgccca tcgagctgac cgcggacctg ctcgagcgcg
     1261 gccgggagcg gttcaacatc ttctgctcgc cgtgccacaa cttcaacgcc gacggcctcg
     1321 gcgctaccgt cctcaagggg ttcccgcagc ctacggactt cactacgacg cagcgcctgc
     1381 tcgacgcccc ggtcggctac ttctacaacg ccatgacgaa cgggttcgga cgcatgtaca
     1441 gctacgcctc gcgcatcccc gtcgcggacc gctgggccat cgccgcctac gtcaaggcgc
     1501 ttcagctcag tcagaacgcg ggcgtagtgg acataccggc cggcgtgacg ctcgctccaa
     1561 gcgcgacgga ggccaaccga tgagttcgct caagctcccc cccatcaaca cgagcaaggc
     1621 gcaggtcggc ggcttggtgg tcggggcggt ggcgctgctg atcgccgtcg tcctcggctt
     1681 cgcgggacag gcagaggggt tcttccagtc gtacctgttc acctacctct tctggctcgg
     1741 cctctcgctc ggctcgctc
//
LOCUS       DAOQYE010000494         1759 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_81551_length_1759_cov_2.593310, whole genome shotgun sequence.
ACCESSION   DAOQYE010000494 DAOQYE010000000
VERSION     DAOQYE010000494.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1759
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81551_length_1759_cov_2.593310"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(13..1395)
                     /locus_tag="PKN52_08205"
     CDS             complement(13..1395)
                     /locus_tag="PKN52_08205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99970.1"
                     /translation="MSVDHAIRLSPSTRAFSPKLLALVAVLILAAAAFGQAVLHDNGP
                     LITNPGEGAGGTDLSVLQTALGLDVYGGGVKPGNGHSLADEFEVTTDGGWQLAGFEIP
                     AYQTNSGLDSTLTGAYFQIWDGPPGEAGSTVLYGDLAVDRLVDSTFSGIYRVRDTDLA
                     NTTRPIMTNVVAADEVYLPRGTYWLEFQVNGDDALAGPWVPPVTVLGETTTGDAYQRT
                     LGAGWNPWVDTGTAATAQGVPFVVLGQDAGAELTPRSSSPLAAEPGDTLVPVLAFSAS
                     TNMAPTVDLTLGIVTLNHQGTGPASVNPGTWASLFLDANGDGVADDPSAPLTAALLAP
                     DGTGTLTVENAGMIAGAGSGFVLAYDLKASLADAALPVVAASLALVLPGWWLARRGKR
                     GLLAALALVALVATACGTPTPPAKKSVQFRAAIASASVTVANGQFAGEVAPVTITGER
                     AVGPVITVEY"
     gene            complement(1504..>1759)
                     /locus_tag="PKN52_08210"
     CDS             complement(1504..>1759)
                     /locus_tag="PKN52_08210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HNQ99971.1"
                     /translation="SVGIVPPLLDDALFDEARAIDEELGRGTARALAREEGLLVGVSS
                     GLNVAAALQLAMELGPECTVVTVAVDTGLKYLAGDLFDVR"
BASE COUNT          290 a          617 c          595 g          257 t
ORIGIN      
        1 gcaggcgtgc gctcagtact cgacggttat aaccggcccg acggcccgct cacccgtgat
       61 ggtgacgggc gccacttctc cagcgaactg accgttcgct accgtcacgc tggccgacgc
      121 gatagcggcc cggaactgca cggacttctt ggcgggcggc gtgggcgtgc cgcaggcggt
      181 agccaccaga gcgactagcg cgagcgcagc caacagcccg cgcttgccac gccgcgccag
      241 ccaccagccg ggcagcacga gcgcgaggct cgctgccacc accggcaagg ccgcgtcggc
      301 cagcgaggcc ttgaggtcgt acgcgagcac gaagccgctg cctgcgcccg cgatcatgcc
      361 tgcgttctcg accgtgaggg tgccggtgcc gtcgggggcc agcagcgcgg cggtcagggg
      421 tgccgacggg tcgtctgcca ctccgtcgcc gttcgcgtcg aggaacaggc tggcccaggt
      481 gcccgggttg acggacgccg gtcccgttcc ctggtggtta agagtcacga tgcccagcgt
      541 gaggtcgacc gtcggcgcca tgttcgtcga ggccgagaag gccagcacgg gcacgagcgt
      601 atcgccgggt tccgccgcga gcggggaact cgacctcggg gtcaactctg cacccgcatc
      661 ctgcccgagt actacgaagg ggacgccttg ggcggtcgcc gccgtcccgg tgtcgaccca
      721 agggttccac ccggctccca gagttcgctg ataggcgtcg ccggtggtcg tctcgcccag
      781 caccgtgacc ggcgggaccc acggcccggc gagggcgtcg tcaccgttga cctggaactc
      841 cagccagtac gtgccgcgcg gcaggtagac ctcgtctgcc gcgaccacgt tggtcatgat
      901 cggacgggtg gtgttcgcaa ggtcggtatc gcgcacgcgg tagataccgg agaaggtgct
      961 gtccaccagc cgatccacgg ccaggtcgcc gtacaggacc gtgctgcccg cttctccagg
     1021 cgggccgtcc cagatctgga agtaggcgcc cgttagggtg gagtcgaggc ccgagttcgt
     1081 ctggtacgcg gggatctcga agcccgccag ttgccagccg ccgtcggtcg tgacctcgaa
     1141 ctcgtcggcc aacgagtggc cgttacccgg cttgacccca ccaccgtaca cgtccaggcc
     1201 caacgcggtc tgaagaacgc tcagatccgt gcccccggcg ccctcgccag ggttcgtgat
     1261 cagcggcccg ttgtcgtgga gtactgcctg accgaacgcg gcggcagcca ggatgagcac
     1321 cgccaccaga gcaaggagct tgggagagaa cgcgcgtgtc gatggtgaga gacgtatggc
     1381 atggtcaacg gacatggcgc cacgatagtt agtcatccac cgatccgtag tgaacaaaac
     1441 ccgccgccgg ccgagcgaac gagccgcggt acctggccca ccttcgcggc gcggcaacgg
     1501 tcgctagcga acgtcgaaca ggtctccggc caagtacttc aacccggtat cgactgccac
     1561 ggtgacgacc gtgcactccg gcccgagctc catcgccagt tggagcgccg ccgccacgtt
     1621 caagccgctc gacacgccga cgaggaggcc ttcctcgcgc gctagcgccc gggccgtacc
     1681 gcggccgagt tcctcatcga tagcgcgcgc ctcgtcgaag agcgcatcgt ccaacagcgg
     1741 cggcacgatg ccgaccgac
//
LOCUS       DAOQYE010000495         1754 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_81936_length_1754_cov_3.078870, whole genome shotgun sequence.
ACCESSION   DAOQYE010000495 DAOQYE010000000
VERSION     DAOQYE010000495.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1754
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81936_length_1754_cov_3.078870"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="PKN52_08215"
     CDS             <1..325
                     /locus_tag="PKN52_08215"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNQ99972.1"
                     /translation="RAREIDPWLEEGLGALKDKHRVIGDVRGVGAFYTIELVKDRDTK
                     EPLVPWYGGDNAPIGKLTGALRNEGVYAFGRYNVLLVTPPLTIKRDELQVGLDGLDRA
                     LKVLA"
     gene            361..1290
                     /locus_tag="PKN52_08220"
     CDS             361..1290
                     /locus_tag="PKN52_08220"
                     /inference="COORDINATES: protein motif:HMM:NF016173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HNQ99973.1"
                     /translation="MQEESLSQPVTSRLTRYALFMVATIADDERAAAAVRSLCPDLSG
                     LVRAVGFRNLDSGLTCMLGIGSAAWDRLFGQPRPMELHPFREINGVHHAVSTPGDLLL
                     HIRGERMDVCFELAMQIVTRLGAAITVVDETHGFRYFDERDLLGFVDGTENPVGRAAD
                     TATMVGAEDPAFAGGSYVIVQKYLHDLDKWNALPVERQEAIIGRKKLSDIELDDSVKQ
                     KYAHNALTSIYDGDRQLEIVRDNMPFGEIGKGEFGTYFIGYACSPQRIERMLDNMYIG
                     NPPGNYDRILDFSRPITGSLFFLPTATFLDGVS"
     gene            complement(1448..>1754)
                     /locus_tag="PKN52_08225"
     CDS             complement(1448..>1754)
                     /locus_tag="PKN52_08225"
                     /inference="COORDINATES: protein motif:HMM:NF015995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HNQ99974.1"
                     /translation="SRGEPIIVTSWRPHWMWAAYDLKYLADPLGVFGDAEQVETAANN
                     DFVANGPAEVIDFLRAFQWTGADVGDVMLKVRGGMEPAAAAAEWISENQELVDSWFN"
BASE COUNT          286 a          633 c          571 g          264 t
ORIGIN      
        1 gcgggcgcgc gagatcgacc cgtggctcga ggaaggcctc ggcgccctga aggacaagca
       61 ccgcgtcatc ggcgacgtgc gcggcgtcgg cgccttctac accatcgagc tggtgaaaga
      121 ccgcgacacg aaggagcccc tcgtgccctg gtacggcggc gacaacgccc ccatcggcaa
      181 gctcacgggc gccctgcgca acgagggcgt ctacgcgttc gggcgctaca acgtcctgct
      241 cgtcaccccg cccctcacca tcaagcgcga cgagctgcag gtcggcctcg acgggctcga
      301 ccgggcgctg aaggtgctcg cctgagcgac catgatcggc agagcgctcc cccgccgagc
      361 gtgcaggagg agagcctcag ccaacccgtc acctccaggc tcacgcgcta cgcgctgttc
      421 atggtggcga cgatcgcaga cgacgaacgg gcggccgcgg ccgtccgttc gctctgcccc
      481 gacctctcag gcctcgtgcg ggcggtgggc ttccgcaacc tcgactcggg cctgacctgc
      541 atgctcggca tcggctccgc ggcgtgggac aggctcttcg ggcagcctcg ccccatggaa
      601 ctccacccct tccgcgagat aaacggcgtg caccacgccg tctccacgcc cggcgacctc
      661 ctcctccaca tccgcgggga gcgcatggac gtctgcttcg agctcgccat gcagatcgtg
      721 acgaggctgg gagcggccat caccgtggtc gacgagaccc acggcttccg gtacttcgac
      781 gagcgcgacc tcctgggctt cgtcgacggg acggagaacc cggtggggcg ggccgccgac
      841 acggccacca tggtcggcgc cgaggacccg gcgttcgccg gcggcagcta cgtgatcgtc
      901 cagaagtacc tgcacgacct cgacaagtgg aacgcgctgc ccgtcgagcg gcaggaggcc
      961 atcatcggcc gcaagaagct ctccgacatc gagctggacg actcggtgaa gcagaagtac
     1021 gcgcataacg cgctcacgag catctacgac ggtgaccggc agctcgagat cgtgcgcgac
     1081 aacatgccgt tcggcgagat cggcaagggc gagttcggca cgtacttcat cggctacgcc
     1141 tgctcgccgc agcggatcga gcggatgctc gacaacatgt acatcggcaa cccgccgggc
     1201 aactacgacc gcatactgga cttcagccgc cccatcacgg gcagcctgtt cttcctaccg
     1261 accgccacgt tcctggacgg cgtctcctag gcgaagcccc cgaggctgct ggggcctcgg
     1321 gggcttcgcc cttgggggat cgtgggtagc tctgcggcgc gcggctagct acggggcccg
     1381 tggctgcctt tagggccacg gctgcctcga ggcccacggc tgccttcggg ccgagccgca
     1441 gtccgcctca gttgaaccag ctatcgacca actcttggtt ctccgagatc cactccgcgg
     1501 ccgccgcggc cggctccatg ccgccgcgga ccttcagcat cacgtcgccg acgtcggcgc
     1561 ccgtccactg gaaggcgcgc aggaagtcga tgacctcggc ggggccgttg gcgacgaagt
     1621 cgttgttggc cgccgtttcg acctgttcgg cgtctccgaa cacgcccaac gggtcggcca
     1681 ggtacttgag gtcgtaagca gcccacatcc agtgcgggcg ccaactcgtg acgatgatgg
     1741 gctcccccct cgag
//
LOCUS       DAOQYE010000496         1754 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_81961_length_1754_cov_2.502060, whole genome shotgun sequence.
ACCESSION   DAOQYE010000496 DAOQYE010000000
VERSION     DAOQYE010000496.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1754
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81961_length_1754_cov_2.502060"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            151..>1754
                     /locus_tag="PKN52_08230"
     CDS             151..>1754
                     /locus_tag="PKN52_08230"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNQ99975.1"
                     /translation="MVSTIHGFMIEGLRLNAPSLGLDPGFGLLGEWEAAAIFEEECTG
                     ILMLAQDPGHPLNAAAAVLGGEVAELLARLFAKRSLAPELTFGPSEREAALARLYRAA
                     LAAYDARLGATSLAPGEVERRAIVMLEREPARRRLVERFPRVLVDEYQDVNPLQGEFF
                     ERLAAAGARLELVGDPKQSIYGFRNADVAVFRRALDAAEAHGEIQPPLRESRRHARAP
                     AAFLNRLTTALGERGRGFTERESPVVVAAGEQASVLGTVELHVVEGAAPLNELRGREA
                     QILAERLRVHNAGGIAYEEMAVIARGHAGLARVGVALRSAGVPFVILQGRGYYERSEI
                     RDVYHALSVGIDPVGTGLLPFLRGPFGGLELGEIAEVALAEPEARLTLIGERWPAVAR
                     RIERLAELARSTPLAAVTGLLREPILGGRRFQEYLPRRGRENVDALLFEVAGQAPRDV
                     ALFLDRLDLLARMGDAGDVPQSGGGVRLLTIHASKGLEFRLAAVFDTGAWRGDGREPF
                     LVEPGTGAVRLAGEPGFEEALVASEA"
BASE COUNT          216 a          651 c          645 g          242 t
ORIGIN      
        1 tcgccgaaac cgttcaccag cccggaaaac gggccgacgt tggcgtccgc aagccagttc
       61 gaaagccgct gctcgtggcc cccgaagaca agcgcgtcag tgatcgctcc ggccgtcttc
      121 gcgcgcgcgc gtgccgagtt ggacggcgcc gtggtcagca ccatccacgg cttcatgatc
      181 gagggcctgc gcctgaacgc gccgagcctc ggcctcgatc cgggcttcgg cctcctcggc
      241 gagtgggagg cggccgccat cttcgaggag gagtgcaccg gcatcctcat gctcgcccag
      301 gaccccggcc accccctcaa cgcggcggcc gcggtgctcg gtggcgaggt ggcggagctc
      361 ctcgcgcggc tgttcgccaa acgctcgctc gcgcccgagc tgaccttcgg cccttcggag
      421 cgtgaagcgg cgctggcccg gctctaccgc gccgcgctgg cggcctacga cgcgcggcta
      481 ggcgctacca gcctcgcgcc gggcgaggtc gagcgccgcg ccatcgtgat gctcgagcgc
      541 gaaccggcgc gtcggcggct cgtagagcgc ttcccgcgcg tgctcgtcga cgagtaccag
      601 gacgtgaacc cgctgcaggg cgagttcttc gagcggttgg ccgccgccgg cgccaggctc
      661 gagctggtgg gcgaccccaa gcagtccatc tacggcttcc gcaacgccga cgttgcagtc
      721 ttccggcggg cactggacgc cgccgaggcg cacggggaga tacaaccgcc cttgcgcgag
      781 agccggcgcc acgcccgcgc tccggcggcg ttcctgaacc gcctcaccac ggccctcggc
      841 gagcggggcc gcggcttcac cgagcgggag tccccagtcg tggtcgcggc cggcgagcag
      901 gcgtccgtgc tcggcaccgt ggaactccac gtcgtggagg gcgccgcgcc gctcaacgaa
      961 ctgcggggcc gcgaggccca gatcctggcc gagcggctgc gcgtccacaa cgcgggcggg
     1021 atcgcgtacg aggagatggc cgtcatcgcc cgcgggcacg ccgggctcgc gcgcgtgggc
     1081 gttgccctgc gctcggccgg cgtgccgttc gtcatcctgc aggggcgcgg ctactacgag
     1141 cgcagcgaga tccgcgacgt ctaccacgcc ctgtccgtgg gcatcgaccc ggtagggacc
     1201 gggctcctgc cgttccttcg cggaccgttc ggcggtctgg agctggggga gatcgcggag
     1261 gtcgcgctgg ccgagccgga ggcgcgcctg accctcatcg gcgagcgctg gcccgcggtg
     1321 gcgcgccgga tcgagcgcct ggccgagttg gcgcgctcca cccccctcgc ggcggtgacg
     1381 gggctcctgc gcgagccgat cctgggcggc aggcgcttcc aggagtacct gccgcgccgc
     1441 ggccgcgaga acgtcgacgc gctcctcttc gaggtggccg gccaggcgcc gcgcgacgtg
     1501 gccctgttcc tcgaccgcct cgatctcctc gccaggatgg gcgacgccgg cgacgtgcca
     1561 cagagcggcg gcggcgtccg gctgctcacc atccacgcct ccaagggcct ggagttccgt
     1621 ctggccgccg tcttcgacac cggggcttgg cgcggcgacg gtcgagagcc gttcctggtc
     1681 gaaccgggta ccggcgccgt gcgcctggcg ggagaaccgg gcttcgagga ggccctggtg
     1741 gcctccgagg ccag
//
LOCUS       DAOQYE010000497         1753 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_82045_length_1753_cov_2.142521, whole genome shotgun sequence.
ACCESSION   DAOQYE010000497 DAOQYE010000000
VERSION     DAOQYE010000497.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82045_length_1753_cov_2.142521"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            184..1245
                     /gene="rodA"
                     /locus_tag="PKN52_08235"
     CDS             184..1245
                     /gene="rodA"
                     /locus_tag="PKN52_08235"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HNQ99976.1"
                     /translation="MTRVDFALPLVTLLLITVGLFTLFSAAPSDEFMRQVVFLGASVV
                     VIGLTLWAGKNRVLRLTPYVYVASLGLLVLTFLIGTEVNGAKSWLYLGPLPGFQPSEL
                     AKLALILVLAQTLHERPIRRLQDYARVAVLALPPIGLVLIEPDLGSALVLVAITAGVV
                     LVRGVPWRHLVLGVLLVGIAVPTVVLPNLKPHQVERLVSFADPYRDPQGTGYQVIQST
                     IAVGSGGLLGKGYRAGTQSQLGFIPYRQTDFIFPVLAEEGGFVASVGLLVLYGLLFWR
                     LLAMAAECPFERDQLLIVGVVVLIGFQVLVNVGVTLGVAPVTGITLPLVSYGGTSLLS
                     TLIALTFAWVVYRDRFADW"
     gene            1330..>1753
                     /locus_tag="PKN52_08240"
     CDS             1330..>1753
                     /locus_tag="PKN52_08240"
                     /inference="COORDINATES: protein motif:HMM:NF013999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNQ99977.1"
                     /translation="MSSSDETTAAPVGSGEATGQALQTAGSEELRAQVLEALKVVHDP
                     EIPVNIYELGLIYKRLGQTDRAATEFQEASATITADTAETHINLGLAYRSQNRLADAE
                     AELGRALELDPQNAEANYCLALLYYDRGQPNMAVVKLEE"
BASE COUNT          252 a          647 c          548 g          306 t
ORIGIN      
        1 aggcgtcgcg ctcgagcagg acgccgccgc gcaggcggtc gactcggagg tgccgggcga
       61 cccagccggc tcggcgtcgc cgacccccct ggtgatcggc ctgctcatcg cggcgttcgc
      121 cgccctgctg gccgagtggt ggctctacgc cggctcacgc aggcggctgg taggctgacg
      181 ggcgtgacga gagtcgactt cgccctgccg ctcgtcaccc tcctcctgat cacggtcggg
      241 ctcttcaccc tgttcagcgc cgctcccagc gacgagttca tgcgtcaggt cgtcttcctc
      301 ggagccagcg tcgtcgtcat agggctgacc ctgtgggcgg gcaagaaccg ggtgttgcgg
      361 ctcaccccct acgtctacgt cgccagcctc ggtctcctgg tcctaacgtt cctgataggc
      421 acggaggtca acggggccaa gtcttggctc tacctcggtc ccctccccgg gttccagcct
      481 tccgaactag ccaagctggc tctcatcctc gtcctcgccc agacgcttca cgagcggccg
      541 atccgccggc tgcaggacta cgcgcgcgtg gcggtactcg cgttgccgcc catcggtctc
      601 gtcctgatag agccggacct cggtagcgcc ctagtgttgg tcgccatcac ggccggcgtg
      661 gttctcgtca ggggcgtgcc gtggcgccac ctcgttctcg gcgtactact ggtaggcatc
      721 gccgtgccga cggtggtgct tccgaacctc aagccccacc aggtcgagcg cctcgtctcc
      781 ttcgcggacc cctaccgcga cccgcagggc accggctatc aggtcatcca gtccaccatc
      841 gcggtcgggt caggcggact gctcggcaag gggtaccgcg cgggcacgca gtcgcagctc
      901 ggcttcatcc cgtaccgcca gacggacttc atcttcccgg tcctagccga ggaaggcggg
      961 ttcgtcgctt ccgtcggcct gctcgtgctc tacggcctgc tcttctggcg gctccttgcc
     1021 atggcggcgg agtgcccgtt cgaacgcgac cagctcctca tcgtgggcgt cgtcgttctc
     1081 atcggcttcc aggtcctcgt aaacgtcggc gtgaccctcg gggtggcgcc cgtcaccggc
     1141 atcaccttgc ccctcgtctc ctacgggggc accagcctcc ttagcaccct catcgccctg
     1201 accttcgctt gggtcgtata ccgcgaccgc ttcgccgact ggtgaacgcc gtggtcgaac
     1261 ggcgaaggcg agcgggctcc gaccgcgggg ctacggcgcg ccgcgtgccg ttctgttaac
     1321 ttcacatgta tgagtagcag cgacgagacg accgcggcgc ccgtcgggtc aggcgaggcg
     1381 accggacagg cgctccagac cgccggcagc gaagagttgc gcgcgcaggt gctggaagcc
     1441 ctcaaggtcg ttcacgaccc cgagatcccc gtcaacatct acgagctcgg cctgatctat
     1501 aaacggctgg gccagaccga ccgggcggcc accgaattcc aggaggcctc tgccaccatc
     1561 acggccgaca ccgccgagac ccacatcaac ctcggcctgg cctaccgcag ccagaaccgc
     1621 ctggccgacg ccgaggccga gctcggccgg gccctcgagc tcgatccgca gaacgccgag
     1681 gccaactact gcctggccct gctctactac gaccgcggcc agccgaatat ggccgtcgtc
     1741 aaactggagg aag
//
LOCUS       DAOQYE010000498         1749 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_82282_length_1749_cov_3.432113, whole genome shotgun sequence.
ACCESSION   DAOQYE010000498 DAOQYE010000000
VERSION     DAOQYE010000498.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82282_length_1749_cov_3.432113"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="PKN52_08245"
     CDS             complement(<1..283)
                     /locus_tag="PKN52_08245"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNQ99978.1"
                     /translation="MGRLKEILTQVPEVADGPETDYGLRTVVGRIEFKDVSVAYGGVK
                     ALDHVSFVIEAGEAVGITGRTGSGKTLIVNLIARVIDPDEGQILIDGIDN"
     gene            complement(271..1209)
                     /locus_tag="PKN52_08250"
     CDS             complement(271..1209)
                     /locus_tag="PKN52_08250"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HNQ99979.1"
                     /translation="MLKTLRDLLPYVRRHRLKLVLGMGLIVTNGFFSTLTPIIPGRAI
                     DEFRNRSMTMDRLWLYALALLGVAVAAGLAMVFVRRIFMYTSWEVQFDVRHDIFKHFT
                     RLDGGYFDRTRVGDLMARLTADLGAVRMLAGVAVFQGLNTLFLFAFTFYRMFNLSVQL
                     SLITLATAPLTSLAFYLILRVVHRRYQYVQEQFSNVSAMAQENFSGIRVVKGFGIEQR
                     EVDTFGRLNNEFVKRNLALVRVDGPLFPMMEFLFGLTMSLLLLVGGRLVLGIGGDLTV
                     CEFSSFVFLYEGIQWPMISLGWIGSLLQRGTTSWGA"
     gene            complement(1371..>1749)
                     /locus_tag="PKN52_08255"
     CDS             complement(1371..>1749)
                     /locus_tag="PKN52_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99980.1"
                     /translation="LGNPGLPEGVVAQDPVPGGPAGAARTLVITVNAHPVRLSTSGVY
                     AVVRGPEPRTVEYAWAIQPGIGLLQAEVWASDLEGRRILVSRASVRGGDILRGEWRST
                     FVGPVRFELLLGGVPYGEPLLVP"
BASE COUNT          340 a          564 c          578 g          267 t
ORIGIN      
        1 tgttgtcgat gccgtcgatg aggatctgac cctcgtccgg gtcgatgacg cgcgcgatca
       61 ggttcactat cagcgtcttg ccagagcccg tgcgacccgt gatgcccacg gcctcgccag
      121 cctcgatgac gaagctgacg tggtccagcg ccttgacccc gccgtatgcc acgctcacgt
      181 ccttgaactc gatgcgccca acgaccgttc ggaggccgta gtcggtctcc ggcccgtcgg
      241 cgacttcagg tacctgggtg aggatctcct tcaggcgccc cacgacgtgg tgccgcgctg
      301 gaggaggctg ccgatccaac cgagcgagat catgggccac tggatgccct cgtaaaggaa
      361 gacgaagctc gagaactcgc agacggtcag gtccccaccg atgccgagga ccaaccgccc
      421 acccaccagc aagaggagcg acatggtgag tccgaagagg aactccatca tggggaagag
      481 cggcccgtcc acgcgaacga gcgccaggtt gcgcttcacg aactcgttgt tgaggcgacc
      541 gaacgtgtct acctcgcgtt gctcgatgcc gaagcccttg acgacacgga tgccggagaa
      601 gttctcctgg gccatggcgg agacgttgga gaactgctcc tggacgtact ggtagcggcg
      661 gtggacgacg cgcaggatga ggtagaaggc cagcgaggtg agcggggcgg tggcgagcgt
      721 tatcagggag agttgaacgc tcaggttgaa catccggtag aacgtgaagg cgaacaggaa
      781 gagagtgttg agcccctgga acacggcgac gccggcgagc atgcggacgg ccccgagatc
      841 ggccgtgagg cgggccatga ggtcgccgac gcgcgtgcgg tcgaagtagc ctccgtccag
      901 cctcgtgaag tgcttgaaga tatcgtgccg gacgtcgaac tggacttccc agctcgtgta
      961 catgaatatg cgcctgacga aaaccatcgc cagcccggcc gccacggcca cgcccaagag
     1021 ggccagggca tagagccata gccggtccat cgtcatactc cggttcctga actcgtcgat
     1081 ggcccgcccg gggattatgg gggtcagggt agagaagaag ccgttggtaa cgataagccc
     1141 catgccaagc acgagcttga ggcggtgcct cctgacgtac ggcagcaggt ctctcaatgt
     1201 cttgagcaca tgactcctcg gtccccctga cgcgcggccg acgaccgccc ccgctgcagc
     1261 gcatggaggc ggtcgtcagg cgaacacttc gttctcgacg cccgctcggt tcacattcat
     1321 aggtggacgc cgaaattacc ggcggcgact ataaccgcca ggtagaaaaa tcatggtacc
     1381 agtaacggct cgccgtaggg aaccccgccg agcaacagct cgaaccggac cggtccgacg
     1441 aaagtcgagc gccactcgcc tcgcaggatg tcgccgccgc gcacggaggc ccggctgacg
     1501 aggatgcggc gcccctcgag gtcggaggcc cacacctcgg cttgcagcaa gccgatgccc
     1561 ggctggatgg cccaggcgta ctcgaccgtg cgcggttccg gcccgcgcac gacggcgtat
     1621 acgccgctcg tggagagccg caccgggtgg gcgttgacag tgatcaccag ggtgcgcgcc
     1681 gccccggccg gcccccccgg cacagggtcc tgggcgacca caccctcggg caaccccggg
     1741 ttgccgagc
//
LOCUS       DAOQYE010000499         1749 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_82305_length_1749_cov_2.801063, whole genome shotgun sequence.
ACCESSION   DAOQYE010000499 DAOQYE010000000
VERSION     DAOQYE010000499.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82305_length_1749_cov_2.801063"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /locus_tag="PKN52_08260"
     CDS             complement(<1..741)
                     /locus_tag="PKN52_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99981.1"
                     /translation="MTEDGSTSSRQRADTSRGLRRSSTLRLSSAGLGLALLTLALTAC
                     PRPEAEKLDFDTDPRILRGPYAGYVDTRRVPHAMAIAGDASLLAMAWPDSLELRNPAT
                     SELVATLDPPSDEELWRVELSIDATGTLVAGILNYDVALWSGHDGQLVRTFDVSGLLT
                     ACEDCGPELLSISPTGGSLAVAGRTPGILVLDTTTGELARELETAGDEVGLVAFSAGG
                     ELLLSASRISTIGYALRVWDASTYEVVFE"
     gene            complement(738..>1749)
                     /locus_tag="PKN52_08265"
     CDS             complement(738..>1749)
                     /locus_tag="PKN52_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HNQ99982.1"
                     /translation="DPGGRLGDCLYCGAGAISLSPSAGLLAVVGDAPVVILVDPSTGT
                     IGDELATEGRRVEMVSFNADGTLLATASYLSDTRYALRVWDTNTYDVVFERTGTVDRR
                     HFPRFAFSADGQRLAVSSDTRLELFDLVGGRQVLQFAESESPWFRALNADGSQVVIGK
                     LTEQGSTLDIIEVATGATLARVKIALRAQSTWSPDGRYLLLGSQLLTADSFSAVRDYT
                     LGQLHDLELTATALYVDTQSYDVSGSVSIDGGPASEFTGTVVGNETQRYVQPQSRAPR
                     PAELELELHAYPWTLHARQLHRGFGTEPSHEGEWWGNMTDDSHEGTAYWYWYPFELRR
                     AE"
BASE COUNT          298 a          566 c          594 g          291 t
ORIGIN      
        1 ctcgaagacg acctcgtagg tggaggcgtc ccagaccctc aacgcgtagc cgatggtgga
       61 gatgcgggac gcggacaaca agagctcgcc gccggcgctg aaggcgacca gcccgacttc
      121 gtctccggcc gtctccagtt cgcgtgcgag ctcgccggtc gtcgtgtcga gcacaaggat
      181 accgggagtc cggccggcca cggccaggga cccgcccgtg gggctgatag acaggagttc
      241 cggcccacaa tcctcacagg ccgtcagaag accgctgaca tcgaaggtcc tcacgagctg
      301 cccgtcatga ccgctccaga gtgcgacgtc gtagttgagg atgcccgcga cgagcgttcc
      361 ggtggcatcg atgctcagtt cgacccgcca gagctcttcg tccgatggcg ggtcgagggt
      421 cgcgactagc tccgaggtcg ccgggttcct cagctcgagc gagtctggcc acgccatggc
      481 gagcagcgag gcgtcgccag cgatggccat ggcgtgcggc acccggcgcg tgtccacgta
      541 gccggcgtac ggaccccgca ggatccgcgg gtccgtgtcg aagtcgagtt tctccgcttc
      601 tggcctcggg caggccgtca gcgccagcgt gaggagcgcg aggccgagtc cggccgagga
      661 aagtcggaga gtcgaggagc gtctgaggcc gcgagaagtg tcagcgcgct gcctgctgct
      721 cgttgatccg tcttcagtca ttcggctctc cgcaactcga acgggtacca gtaccagtac
      781 gcggtgcctt cgtgagagtc gtccgtcatg ttcccccacc attcgccttc gtggctgggc
      841 tccgtgccga acccgcgatg gagctgccgc gcatgcagcg tccacgggta cgcatgtagt
      901 tctagctcga gttccgcggg ccgaggagcg cgcgactgcg gttgcacgta gcgttgcgtc
      961 tcgttgccga ccacggtccc ggtgaactcg ctggccgggc cgccgtcgat gctgaccgag
     1021 ccggagacgt cgtacgactg cgtgtcgacg tacagcgccg tggccgtcaa ctccaggtcg
     1081 tggagctggc cgagggtgta gtcgcgaacg gcgctgaagc tgtccgccgt gagcagctgc
     1141 gagccaagga gcaggtaacg accgtccgga ctccaggtcg attgggcgcg gagtgcgatc
     1201 ttgactcgcg cgagggtggc gcccgtggca acctcgatga tgtcgagagt cgaaccttgc
     1261 tcggtcagct tccctatcac cacctgagaa ccgtcagcgt taagggctcg gaaccaagga
     1321 gactccgact ccgcgaactg aaggacctgc ctgccaccca ccaggtcgaa aagctcgagt
     1381 ctggtgtccg aactgactgc cagccgctgt ccgtcagcgc tgaaggcgaa ccgcgggaag
     1441 tgtctgcggt cgaccgttcc ggtgcgctcg aacacgacgt cataggtgtt cgtgtcccac
     1501 acccgcaggg catagcgggt gtccgacagg tatgacgcgg tggccagcag ggttccgtcg
     1561 gcgttgaagg agaccatctc gacccgcctc ccctcggttg cgagttcgtc accgatggtg
     1621 cctgtagacg ggtctacgag gatgaccacc ggcgcatctc cgaccacggc cagcaagccg
     1681 gcggaagggc tgagggatat ggcgcctgcg ccgcagtaca gacagtcgcc caaccgaccc
     1741 ccgggatcg
//
LOCUS       DAOQYE010000500         1748 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_82381_length_1748_cov_2.834022, whole genome shotgun sequence.
ACCESSION   DAOQYE010000500 DAOQYE010000000
VERSION     DAOQYE010000500.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1748
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82381_length_1748_cov_2.834022"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1012)
                     /locus_tag="PKN52_08270"
     CDS             complement(<1..1012)
                     /locus_tag="PKN52_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99983.1"
                     /translation="MTTSVYQAAVMELESVLSPRIVSRALKDGLRQLGRTPETADLSD
                     IEKVMKSQVFRQLQVMMPVTQAKETVATILEHLTGSSVGAGYSESASEASLEAVGQRL
                     ATIQAELRPFNLYFEWPEVQKLRAQVQLVDSEHGAGRSALAVLQEAEEQLGVVKQKLE
                     DQLVLQARDLGELSEMLEAVRELGGTRVRRLETLVNQVQAAQTQRQLAPAELERAHRQ
                     ARELRRTMEVEAQAGEASAATGPATDAVDDAAGAAFDAASGAEPAASDAAYGAASGLQ
                     VQAMEPDPVEGDAGDLDAATAALVDMLEFGPTTTDQASEQTPPVRDAEFEDRMRLLDI
                     ASE"
     gene            complement(1068..>1748)
                     /locus_tag="PKN52_08275"
     CDS             complement(1068..>1748)
                     /locus_tag="PKN52_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99984.1"
                     /translation="HAALAGFTTTYDSAVAPSLQQVAPDVAQGTRDLLASLADRARLS
                     TTDASLVGARTGIIRAAFAGEPTSVADTVETAVAGTWSGLPREVVYIVLALLAIIPLV
                     LLKMAFGGSNRNWRLVGWALFLLLVPTFYQGIVALVDLLSRYVDMSWLPDLGRWSMFG
                     GITGQAVWAVLVLLALVLAIIGLRGICVQFGLLGGQRRKSVAPAPAAAVAKKSSTGNT
                     TIDWDEEF"
BASE COUNT          258 a          595 c          622 g          273 t
ORIGIN      
        1 gttccgaggc gatgtcgagg agccgcatgc ggtcctcgaa ctccgcgtct cgcacgggcg
       61 gggtctgttc gctcgcctga tccgtggtcg tcggaccgaa ctccagcatg tcgacgagcg
      121 ccgcggtcgc cgcatcgagg tcgccggcat cgccctcgac cgggtccggc tccatggcct
      181 ggacctgcag gccgctcgcg gcgccgtagg cggcgtcaga ggctgccggt tcggcgccgg
      241 aggccgcgtc gaacgctgcg cctgccgcgt catcgaccgc atctgtggcc ggcccggtcg
      301 cggcgctcgc ctcgcccgct tgcgcctcca cctccatggt gcgcctgagc tcgcgcgcct
      361 ggcggtgggc gcgctcgagc tccgcgggcg ccaactggcg ttgggtctgc gcggcctgca
      421 cctggttgac gagcgtctcg aggcgcctta cgcgcgtgcc gccgagttcg cggacggcct
      481 cgagcatctc cgagagctca cccaggtcgc gcgcctgcaa gacgagctgg tcctcgagct
      541 tctgctttac gaccccgagc tgctcctccg cctcctggag caccgcgagc gcggaccgtc
      601 cggcgccgtg ctcgctgtcg accagttgca cctgcgcccg gagcttctgg acctcgggcc
      661 actcgaagta gaggttgaac ggccgcaact cggcctggat ggtggcgagc cgctggccga
      721 ccgcttccag gctcgcctcg gaggcgctct cggagtagcc ggcaccgacc gaggaacccg
      781 tgaggtgctc gaggatggtg gcgacggtct ccttggcctg cgtcacgggc atcatcacct
      841 gcagctggcg gaagacctgg gacttcatga ccttctcgat atcggagagg tcggccgtct
      901 ccggggtgcg cccgagctgt cgcaggccgt ccttgagcgc tcgcgagacg atcctcggtg
      961 agagcaccga ctcgagctcc ataacggcgg cctggtagac ggaagtggtc atgacggtca
     1021 tgtggcgccg ccaccactag accgctcacc gcggcagccg tgcggcatca gaactcctcg
     1081 tcccagtcga tggtggtgtt gccggtactg ctcttcttcg ccaccgcggc ggccggcgcc
     1141 ggggcgacgc tcttcctcct ctgacccccg aggagaccga actgcacgca gatgccgcgc
     1201 aggccgatga tggcgagcac gagcgcgagg aggactagga cggcccatac ggcttggccg
     1261 gttatgccac cgaacatcga ccagcggccg aggtccggga gccaggacat gtcgacgtag
     1321 cgggagagga ggtcgaccag ggcgacgatc ccctgataga aggtgggtac gagcaggagg
     1381 aagagcgccc aaccgacgag ccgccagttg cggttgcttc cgccgaacgc catcttcagg
     1441 agcaccagtg ggattatggc gaggagcgcg agcacgatgt acacgacttc gcggggcagg
     1501 ccggaccatg tgcctgcgac cgccgtctcg accgtatcgg cgacgctcgt cggctcaccg
     1561 gcgaaggccg ccctgatgat cccggtgcga gcgccgacca gcgaggcatc ggtggtagac
     1621 agacgcgccc ggtcggctag cgaggcgagc agatcgcggg tgccttgcgc gacgtcggga
     1681 gcgacctgct gaaggctcgg cgcgacggcg ctgtcgtagg tcgtggtgaa gccggcgaga
     1741 gcggcatg
//
LOCUS       DAOQYE010000501         1748 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_82391_length_1748_cov_2.492026, whole genome shotgun sequence.
ACCESSION   DAOQYE010000501 DAOQYE010000000
VERSION     DAOQYE010000501.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1748
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82391_length_1748_cov_2.492026"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="PKN52_08280"
     CDS             <1..323
                     /locus_tag="PKN52_08280"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNQ99985.1"
                     /translation="AVAFRRSRASIWRRVVFPQLTPYLVGTARGALALTWKMVVLGEL
                     LGRTSGVGYQISFYFQFFNMTGVLAYGVTMMLLLAVIDLGLMGAVQHFAFRWRAPVKV
                     GAVG"
     gene            323..1072
                     /locus_tag="PKN52_08285"
     CDS             323..1072
                     /locus_tag="PKN52_08285"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ99986.1"
                     /translation="MAELTARGITKSYPTGTAGERRAIAGVDLHVASGELVCVIGPSG
                     CGKTTLLNVLAGLDAPDSGTVEIAGGRGPTPVISYVFQEPRLLPWSTVSSNLRFVLDD
                     QKGARALVAAWLERVGLAGRGDDYPRQLSIGQRQRVAVARALVVQPDVLLMDEPFSAL
                     DELTASRMRIELLALWEELRCTVVFVTHNPLEAALLADRILVMSPGPGRIVREIRPAD
                     VLARPRDPDDPRLWELSRQAVAALRGGSEGR"
     gene            complement(1116..>1748)
                     /locus_tag="PKN52_08290"
     CDS             complement(1116..>1748)
                     /locus_tag="PKN52_08290"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ99987.1"
                     /translation="SAAALRPADRPLLREVVVSPAPMFSVTIGKLLGGATISMIQGSI
                     PLLFAPLIGVRLSVLGVLGAWGVMFCLGSFMTAVGVIIATRLKTFEGFGSISNGVIQP
                     LYFLSGSIFPLRGVIGGVGFLDIPDHLRDELRRIGVFSIGGGWVVQLPEWLKVLVYVN
                     PVSYQLDLMRRVLLRFHQLPMVADVLVTVLAPLLAAVVAAALMRRMTAQR"
BASE COUNT          290 a          597 c          599 g          262 t
ORIGIN      
        1 tggccgtggc cttccgccgc tcgcgtgcga gcatctggcg ccgcgtcgtc ttcccgcagc
       61 tgacgcctta cctggtgggc acggcgcgtg gcgcgctggc gctcacgtgg aagatggtcg
      121 tcctcggcga gctgctcgga cgcacgtcgg gggtcggcta ccagatctcg ttctacttcc
      181 agttcttcaa catgaccggt gtcctcgcct acggcgtgac gatgatgctc ctgctcgccg
      241 tcatcgacct tggcctgatg ggggccgtcc aacatttcgc cttccgttgg cgtgcgccag
      301 tcaaggtcgg cgcggtcggc tgatggcgga gctgacggcg cgcggcatca cgaagtcgta
      361 cccgaccggc accgccggcg agcggcgtgc catcgccggc gtggacctcc acgtcgcgag
      421 cggtgagctg gtgtgcgtga tcggaccatc gggctgcggc aagaccacgc tcctcaacgt
      481 gctcgccggg ctggacgcac cggatagcgg gaccgtcgag atcgcgggcg gccgtggccc
      541 gacgcccgtc atcagctacg tgttccagga gccgcggctc ctaccgtggt cgacggtcag
      601 ctccaacctg cgcttcgtcc tggacgacca gaagggcgcg agggcgctcg tagccgcatg
      661 gctcgagcgc gtcgggttgg cgggccgggg cgacgactac cctcgccagc tctccatcgg
      721 acagcgtcag cgcgtcgcag tcgcgcgcgc gctggtcgtg caaccggacg ttctgctcat
      781 ggacgaacct ttctccgccc tcgacgagct cacggcctca cgcatgcgca tcgagctgct
      841 agcgctctgg gaggaactcc gctgcaccgt cgtcttcgtc acccacaacc cgcttgaagc
      901 ggctctgctg gcggatcgga tactcgtcat gagccccgga ccgggacgca tcgtgcggga
      961 gatccgtcca gccgacgtgc tcgcacgccc ccgagacccg gacgacccgc ggctctggga
     1021 gctgtccagg caggcggtag cggccctgcg gggcggatcg gaggggcgct gaccgcctct
     1081 taggcaacac gggcgacccg ccgtgagccc gcgcctcagc gctgagccgt catcctccgc
     1141 atgagcgcgg ccgcgacgac ggcggccagt agcggagcca agacggtgac caggacgtcc
     1201 gccaccatgg gcaactggtg gaaccgcaac aacacgcggc gcatcaggtc gagctggtag
     1261 ctcacggggt tcacgtaaac gaggaccttg agccattccg gcaactgcac gacccaaccg
     1321 ccgccgatcg agaacacgcc gatacgccgc agctcgtcgc gcagatggtc ggggatgtcg
     1381 aggaaaccga cgccgcctat gacgcccctg agcgggaaga tcgagccgga gaggaagtag
     1441 agcggctgga tgacgccgtt cgagatgctg ccgaagccct cgaacgtctt gagtcgcgtt
     1501 gcgatgatga cgccgaccgc ggtcatgaac gagccgaggc agaacatgac gccccaggcg
     1561 cccagtaccc cgaggacgct taggcgcacg ccgatcagcg gagcgaacaa cagcgggatc
     1621 gacccctgga tcatggagat ggtcgcgcca cccaggagct tgccgatggt caccgagaac
     1681 atgggcgccg gcgatacgac gacctcgcgc agcaggggtc ggtcagccgg acgcagggca
     1741 gcggcact
//
LOCUS       DAOQYE010000502         1747 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_82449_length_1747_cov_3.330378, whole genome shotgun sequence.
ACCESSION   DAOQYE010000502 DAOQYE010000000
VERSION     DAOQYE010000502.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82449_length_1747_cov_3.330378"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1256)
                     /locus_tag="PKN52_08295"
     CDS             complement(<1..1256)
                     /locus_tag="PKN52_08295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ99988.1"
                     /translation="MSTAEAASPLTPKTVVGTWLPLALSWLIMTSELVIAAAFVARMA
                     SPELNLAAWGIVFAISVTIQATAQTLLPTGAALASDAPAYARLRTYAMRLLLALTGVQ
                     LLVALTPLYDLVMRGIGAQREIVDIARLGVLLMVPYAFGTGFRRFQQGVLIRYGDARV
                     VVWGSVLRIACLTLVLTVSAVTGAVSGVVAASAAVITGVLAEALFTQVKVVPVLAGPL
                     QRQTVGAAMTFGRFARFVWPLMVMTVLTMLVQSFVSVALARLPLSLESLAVWPVLWGF
                     LMLWQSPGMAFTEVVISLVRRPGAAPLLRRFTLWAAGGFTLALLAVAATPLARVWFTN
                     VSGLQPELAELAGRPLWFGSLLPALRILISWYQGVIMYSEQTRSIMESILVFLAVGVA
                     GLYLGGTFTSVAGIYVGIVAFSLAFAA"
     gene            complement(1291..>1747)
                     /locus_tag="PKN52_08300"
     CDS             complement(1291..>1747)
                     /locus_tag="PKN52_08300"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNQ99989.1"
                     /translation="RAQEGRASNVNYVFKTSNPKRYRFPTHTNLLVMDRAEATTSESF
                     ISVMEPGEAPPLHKHDDTEQVFYVLEGEGRLEIGPEARFVADLVPGDLVRIPPSTLHR
                     VHCTSAVPLAYLVVDCFPGGRPTVEPTWDSHVATVCHENGWDMSEVVEG"
BASE COUNT          300 a          587 c          646 g          214 t
ORIGIN      
        1 gcggcgaacg cgagcgagaa cgccacgatg ccgacgtaga tcccggcgac gctcgtgaag
       61 gtgccgccga ggtacaggcc ggccacgccg acggccagga agacgaggat ggactccatg
      121 atggagcgcg tctgctcgct atacatgatg actccctgat accagctgat gaggatgcgc
      181 agggccggca ggagcgagcc gaaccagagg gggcgcccgg ccagctccgc cagctccggt
      241 tgcaggccgg agacgttcgt gaaccagacg cgcgcgaggg gcgtcgcggc cacggccagc
      301 agcgccagcg tgaacccgcc tgccgcccac agggtgaacc ggcggagtag cggggcggcg
      361 ccaggccggc gcacgaggct gatgacgacc tccgtgaacg ccatgccggg gctctgccag
      421 agcatgagga agccccacag cacgggccac acggcgagtg actcgagcga gagcggcagg
      481 cgcgcgaggg cgactgacac gaacgactgc acgagcatcg tgaggaccgt catgaccatg
      541 agcggccaga cgaacctcgc gaagcgcccg aacgtcatgg ccgcgccgac ggtctggcgc
      601 tgcagcgggc ccgccagcac gggcacgacc ttcacctgcg tgaagagcgc ctcggccagc
      661 acgccggtta tcacggccgc cgacgcggcc acgacgcctg acacggcgcc ggtgacggcg
      721 gagacggtca ggacgagcgt gaggcacgcg atccgcagca cggagcccca cacgaccacg
      781 cgcgcgtcgc cgtagcggat gagcacgccc tgctggaagc gcctgaagcc ggtcccgaac
      841 gcgtacggca ccatcagcag cacgccgagg cgcgcgatgt cgacgatctc ccgctgcgcg
      901 ccgatgccgc gcatcacgag gtcgtacagg ggcgtcagcg ccacgaggag ctgcacgccc
      961 gtcagggcaa ggaggaggcg catggcgtac gtccgcaggc gcgcgtaggc gggcgcgtcg
     1021 gaggcgaggg cggcgccggt cgggaggagc gtctgggccg tggcctggat ggtcacggag
     1081 atcgcgaaga cgatgcccca ggccgccagg ttgagctcgg ggctcgccat gcgcgccacg
     1141 aacgcggccg cgatgacgag ctccgacgtc atgatgagcc agctaagcgc cagcggcagc
     1201 cacgtgccga cgacggtctt cggggtgagg ggtgaggctg cctctgcggt gctcacgcgc
     1261 gtggagcgtc tgccccgtgg gtcaggcggc tcagccctcc actacttcgg acatgtccca
     1321 gccgttctcg tggcagacgg tcgcgacgtg cgagtcccac gtcggctcga cggtcggccg
     1381 gccgccgggg aagcagtcca cgacgaggta cgcgagcggc acggcggacg tgcagtgcac
     1441 gcggtgcaac gtgctcgggg ggatgcgcac gaggtcgccg ggcacgaggt cggccacgaa
     1501 gcgcgcctcc ggcccgatct cgaggcgacc ctcgccctcc agaacgtaga agacctgctc
     1561 cgtgtcgtcg tgcttgtgca ggggcggcgc ctcgcccggc tccatcacgc tgatgaagct
     1621 ctcgctcgtg gtcgcctcgg cccggtccat caccaggagg ttggtgtggg tcgggaagcg
     1681 gtaacgcttc gggttggagg tcttgaagac gtagttcacg ttacttgccc ttccttcctg
     1741 cgcccgc
//
LOCUS       DAOQYE010000503         1744 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_82686_length_1744_cov_3.342806, whole genome shotgun sequence.
ACCESSION   DAOQYE010000503 DAOQYE010000000
VERSION     DAOQYE010000503.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82686_length_1744_cov_3.342806"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="PKN52_08305"
     CDS             complement(<1..344)
                     /locus_tag="PKN52_08305"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNQ99990.1"
                     /translation="MSLDLHANLETLIADLVRIDSVNPSLDPDHSGETDLARFVSKWC
                     EDRGLQVEWLESYAPGRPSVIITARGTGGGRNVLLYAHLDTVGVKGMASPFEPRVEGG
                     RMYGRGTIDMKA"
     gene            604..>1744
                     /locus_tag="PKN52_08310"
     CDS             604..>1744
                     /locus_tag="PKN52_08310"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNQ99991.1"
                     /translation="MIPVVASTFRKSFNRLTDDEQKLVSSTVMELHLGITTPGQNREK
                     LGGADQSMYSMRVNDDIRMILNFQGQYAVICYVDHHDAAYQWAKGRKLEINEDTGAVQ
                     FVVIDEQVIEVVRTKLVEQEPETVDARHSRRPFADLPSSYLGQLGVPKQWRALVREAS
                     EETFLGELGRHLPEEARENLLSIAAGVKPQLSVKIAGRDPFQHPDAKRQFKIMNVDDD
                     ALRLALFEPWEDWEVFLHPTQQDAVDRRHSGPARVSGGPGTGKTVVAVHRAARLARYG
                     RGRVLLTSFSKTLAERLQEQVNSLLRVEPDALARIDVVHLHQVAVRWYNEHAKRPFQA
                     PQGRRLEELLGRAWNEAKGGGQTLAFAEAEWDMVMDPQGIRDLDTY"
BASE COUNT          344 a          571 c          548 g          281 t
ORIGIN      
        1 ctcgccttca tgtcgatggt gccacgaccg tacatgcggc cgccctccac gcgcggctcg
       61 aacggtgagg ccatgccttt cacacccacc gtatccaggt gggcgtagag caggacgttg
      121 cggccgccgc cagtgccgcg ggccgtgatg atcacgcttg ggcgacctgg ggcgtaggac
      181 tcgagccact cgacttgcag accgcggtct tcgcaccact tggagacgaa gcgggccagg
      241 tcggtttcgc cggagtggtc ggggtctagg gaggggttga cggagtcgat gcggacgaga
      301 tccgcgatca gagtttccag gttggcatgg aggtcgagtg acatcgtcag gttcctctct
      361 ggccgcggag acgggctgca ccaaggtaac gcctggccgg caacggcacg gcctcatgcg
      421 atctagaacc ggtgcccgca tccagcggtc cgccttacca gccatcccat ccgggtgtca
      481 tcgacctcga agtcctcgcg accatcactg ctaggcttcc ggcgcgaacc aggggtaggc
      541 tggcaggaaa gcccagtgag caccatgttt ggttcatttg gacccacgga ggttcgtcgt
      601 cgaatgatcc cagtcgtcgc tagcacgttc aggaagtcct tcaaccgact gacggacgac
      661 gagcagaaac tcgttagctc cacggtcatg gagctgcacc tgggcattac gacacccggc
      721 cagaatcgcg agaagcttgg tggcgccgac cagagcatgt actccatgcg ggtcaacgac
      781 gacatccgca tgatcctcaa cttccagggc cagtacgccg tcatctgtta cgtcgaccac
      841 catgacgccg cctaccagtg ggcgaaaggt cgcaagctgg aaatcaacga ggacacgggc
      901 gccgtccagt tcgtggtcat agacgaacag gtgatcgagg tggtcaggac gaagctcgtc
      961 gagcaggagc ctgagacagt agacgcacgt catagccgtc gccccttcgc ggacctaccc
     1021 tcctcctacc tcggccagct cggagtcccg aaacagtggc gcgcccttgt gcgcgaagcc
     1081 tcggaagaga ccttcctcgg cgagcttggc agacacttgc ccgaagaggc acgcgagaac
     1141 ctcctcagca tcgcagcagg cgtcaaaccg caactttccg tcaagatcgc cggccgcgat
     1201 ccgttccaac acccggacgc caagcgccag ttcaagatca tgaatgtcga cgacgacgcc
     1261 ctgcgcctcg ccctcttcga gccttgggag gactgggagg tcttcctgca cccgacccag
     1321 caggacgcgg tcgaccggag acacagcggc cctgctcgcg tgagcggcgg ccccgggacg
     1381 ggcaagaccg tcgtcgcagt acaccgcgcc gcacgcctcg cgcgctacgg acgagggcga
     1441 gtgctactca cctccttctc gaagaccctg gccgagcgct tgcaggagca ggtcaactcg
     1501 ctactgcgcg tagaaccgga cgcgcttgcc cggatcgatg tcgtccacct acaccaagtg
     1561 gccgtgcgtt ggtacaacga gcacgccaag cggccgttcc aggcgccgca gggccgccga
     1621 ctcgaggaac tcttggggcg agcatggaac gaagcgaagg gcggcggtca gaccctagct
     1681 ttcgcggagg ccgagtggga catggtcatg gacccacagg gcatccgcga cctggacacc
     1741 taca
//
LOCUS       DAOQYE010000504         1743 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_82788_length_1743_cov_2.676540, whole genome shotgun sequence.
ACCESSION   DAOQYE010000504 DAOQYE010000000
VERSION     DAOQYE010000504.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82788_length_1743_cov_2.676540"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="PKN52_08315"
     CDS             <1..473
                     /locus_tag="PKN52_08315"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF012532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HNQ99992.1"
                     /translation="FTQCRANLPGWYGLGAALSAIEPELAAEMYRAWPFFTTMIDFAQ
                     MSLAKADMPIFGAYLGLVDEPLRHFGERIERHHAETLGLVEAITGAPLLEQDPVLARA
                     LQLRNPYVDPISRLQVELLRRLRRQPPEGPDREALSYGVMLSLMGVSAGMRNTG"
     gene            568..1284
                     /locus_tag="PKN52_08320"
     CDS             568..1284
                     /locus_tag="PKN52_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNQ99993.1"
                     /translation="MTVTELLLSGGPILVVIILVSIYAVYLFVERLLKLNRERSDTTS
                     LMARVNAAVRERDLDTALDACERHGGPTGRVLKSALERLPYGRPAVEAAFEEATITEE
                     QNLTRGLTPLAIMAKILPMLGLLGTVTGMIISFSEISLHGSGDAGQLAYGIGQALVTT
                     AAGLIVAIPVLVGHGYLNSLVSKLLGDIDKRREELMGSIVRAVADRRDEGGVTTYRQG
                     SEPQAERNVDMRGVRPAPQA"
     gene            1288..1701
                     /locus_tag="PKN52_08325"
     CDS             1288..1701
                     /locus_tag="PKN52_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNQ99994.1"
                     /translation="MSRARLRRGRGLTIDPELDLTPMVDVVFLLIIFFMVSTTFITVE
                     TGLPVDLPDAQMSVAEPSNLPTVTVMKDGAVYFAGARVNESELVELVRTEINRSGNST
                     IVLRADRELPHGTAVRIMDLIKQAGAQRIAISTGG"
BASE COUNT          298 a          575 c          569 g          301 t
ORIGIN      
        1 cgttcacgca gtgccgggcg aacctgcccg gctggtacgg cctcggagcc gcgctgagcg
       61 ccatcgagcc cgagctggcg gccgagatgt accgcgcctg gcccttcttc accaccatga
      121 tcgacttcgc ccagatgagc ctcgccaagg ccgacatgcc gatcttcggc gcctacctcg
      181 gcctggtcga cgagccgcta aggcacttcg gcgagcgcat cgagcgccat cacgccgaga
      241 ccctcgggct ggtcgaggcc atcaccgggg cgccgctgct cgagcaggac ccggtgctcg
      301 cgcgtgccct gcagctacgc aacccctacg tcgaccccat cagccgcctg caggtcgagc
      361 tcctgcgacg tctgaggaga caaccacccg aagggccgga tcgcgaggcg ctctcgtacg
      421 gggtgatgct ctcgttgatg ggcgtgagcg cgggcatgcg caacacgggc tgagctgact
      481 ggtcggacca atagcgtcgg cgctacgcgt ttctcatgtg cggcgcgcta acatggcgca
      541 agctttaaac cgcgcaagga gcgtggaatg actgtaactg aactacttct gagcggtggg
      601 cccatccttg tcgtcatcat cctggtgtcg atctacgccg tctacctctt cgtcgagcgc
      661 ctgctgaagc tcaaccgcga gcgttccgac accaccagtc ttatggcgcg cgtcaacgct
      721 gcggtccgcg agcgggacct cgacacggcg cttgacgcat gcgagcgcca cggtgggccg
      781 accgggcgcg tcctgaagtc tgcgttggag cggttgccgt acggtaggcc tgcggtcgag
      841 gcggccttcg aggaggccac catcaccgag gagcagaacc ttacgcgtgg cctgactccg
      901 ctcgcgatca tggccaagat cctcccgatg cttggcctcc tgggcactgt cacgggcatg
      961 atcatctcct tctccgagat ctcgttgcac ggctcgggtg acgccggtca gctcgcttac
     1021 ggcatcggtc aggcactcgt tacgacggct gccggcctca tcgttgcgat cccagtcctc
     1081 gtcggtcacg gttacctgaa cagcctggtg tccaagctcc tcggcgacat cgacaagcgt
     1141 cgcgaggagt tgatgggcag catcgtgcga gccgtcgctg ataggcgcga cgagggcggc
     1201 gtgaccacgt acaggcaggg cagtgagccg caggccgagc gcaacgtcga catgcgcggc
     1261 gtgcgtccgg cgccgcaggc ctgaaccgtg tcgagagcga ggctcaggcg cggacgcggg
     1321 ctgaccatcg atccggaact cgatctgacg ccgatggtcg acgtcgtgtt cctcctgatc
     1381 atcttcttca tggtgtccac cacgttcatc acggtggaga cgggcctgcc ggtcgatctg
     1441 ccggatgccc agatgagcgt ggccgaaccc tccaacctgc ccaccgtgac agtgatgaag
     1501 gacggcgccg tgtacttcgc gggcgcccgc gtgaacgagt cggaactggt ggaactggtc
     1561 aggacggaga taaaccgctc aggtaactcg accatagtcc ttcgtgccga cagggagttg
     1621 ccgcacggta cggctgtgcg catcatggat ctcatcaagc aggccggcgc ccagcgcata
     1681 gccatctcta ccggagggtg agggggcagg catgacgagc gacgcgcagc atcaggtgcg
     1741 ccc
//
LOCUS       DAOQYE010000505         1739 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_83103_length_1739_cov_3.018409, whole genome shotgun sequence.
ACCESSION   DAOQYE010000505 DAOQYE010000000
VERSION     DAOQYE010000505.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83103_length_1739_cov_3.018409"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(110..1477)
                     /locus_tag="PKN52_08330"
     CDS             complement(110..1477)
                     /locus_tag="PKN52_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HNQ99995.1"
                     /translation="MTTVVQLLYMLAAVLFILGLKMLGRADSARRGNFVSGGGMLVAV
                     VATLFASGLDFGIIVAGIVVGTAVGVYVARTVKMTSMPEMVALLNGTGGAASMLVGWN
                     EYIQRPDSGTVAAISIFAAVVVGAMTTTGSVLAWAKLSEKIDGKPIMFASQQVVNAVI
                     LGATLVLGAIFVINPAGTYALLIVYLVLGLALGVLVVLPIGGADMPIVISLLNSYSGV
                     AAAAAGFAIGNTVLVVAGSLVGASGLILTQIMCKAMNRSLTNVLFSGFGSGTTASSTA
                     VTGEIKPVSVEDAYYILEAASSVIFVPGYGMAVAQAQHVVKELADLLEKNGAEVRYVI
                     HPVAGRMPGHMNVLLAEANVPYEQLVEPGDVNPGMELVDVAVVIGANDVVNPAAREDE
                     ASPLYGMPIIDVDRARTCFVLKRSMKPGFSGVENPLFYKPNTRMLFGDAKASLGALVS
                     EFKDA"
     gene            complement(1484..1738)
                     /locus_tag="PKN52_08335"
     CDS             complement(1484..1738)
                     /locus_tag="PKN52_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HNQ99996.1"
                     /translation="MLAIFLGFELINKVPSQLHTPLMSGSNAISGITIVGAILATQID
                     GTFGQILALLAIIAATVNVVGGYLVTDRMLGMFRAGKKGE"
BASE COUNT          321 a          559 c          585 g          274 t
ORIGIN      
        1 cacccgaagg cgctcatgcg gtcatcgccg acgccacctc agacgacaac ggggtgccgg
       61 cggagtccgc cggcaccccg ttcccatgct ctgagcagcg ctcggcgagc taggcgtcct
      121 tgaactcaga gacgagcgct ccgagcgacg ccttggcgtc gccgaagagc atgcgggtgt
      181 tgggcttgta gaagagcggg ttctcgacgc cggagaaccc cggcttcatg gagcgcttga
      241 gcacgaagca cgtacgcgcc ctgtcgacgt cgatgatggg catgccgtag agcgggctcg
      301 cctcgtcctc gcgcgccgcc gggttgacta cgtcgttcgc tccgatgacg acggcgacgt
      361 cgacgagctc catgcctggg ttgacgtcgc ctggctcgac gagctgctcg taagggacgt
      421 tggcctccgc caggaggacg ttcatgtggc cgggcatgcg accggccacc gggtggatca
      481 cgtacctgac ctcagcgccg ttcttctcca agaggtcggc gagctccttc accacgtgtt
      541 gggcttgggc aacggccatg ccgtaaccgg gcacgaagat gacactgctt gcagcctcga
      601 ggatgtaata ggcgtcctcg acgctcacgg gtttgatctc gcccgtgacc gcggtggagc
      661 tcgccgtcgt gcccgagccg aagccgctga agagcacgtt cgtcaacgag cggttcatgg
      721 ccttgcacat gatctgggtc aggatgagtc cggacgcccc gacgagggag ccagctacga
      781 cgagcaccgt gttgccgatg gcgaagccgg ccgcggctgc agccacgccg gagtagctgt
      841 tcagaagcga gatgacgatg ggcatgtcgg ccccgccgat cggcaggacg accagcacgc
      901 ccagggcaag tcccaacacc agatagacga tcaggagagc ataggtgccg gccgggttga
      961 tcacgaagat ggccccgagc accagggtgg cgccgaggat gaccgcgttg acgacctgct
     1021 gggaagcgaa catgatcggc ttgccgtcga tcttctctga cagcttggcc cacgcgagca
     1081 cggagcccgt cgtcgtcatg gcgccgacga cgacggccgc gaagatcgag atggcggcga
     1141 ccgtcccgga gtcgggccgt tggatgtact cgttccagcc cacgagcata gaggcggcgc
     1201 cgcccgtgcc gttcaggagc gcgaccatct ctggcatgga cgtcatcttc accgtgcgcg
     1261 ccacgtagac gcccaccgcc gtgcccacga ctatcccggc gacgatgatg ccgaagtcga
     1321 gcccggaagc gaacagcgta gccacgacgg cgacgagcat gcccccgccc gacacgaagt
     1381 tgccgcgccg cgcgctatcg gcgcggccga gcatcttgag gccaaggatg aacagcacgg
     1441 cggcgagcat gtagaggagc tgaacgaccg ttgtcaccct gcttcactca cctttcttgc
     1501 cggcccgaaa catgccgagc atgcggtctg tcaccagata gcccccgacg acgttcaccg
     1561 tcgcggcgat gatggcgagc agggccagga tctgaccgaa ggtcccgtct atctgggtgg
     1621 cgaggatggc cccgacgatg gtgatgcccg agatggcgtt gctgccggac atcaggggcg
     1681 tgtggagctg cgaaggcacc ttgttgatca gctcgaagcc gaggaagatc gccagcacg
//
LOCUS       DAOQYE010000506         1735 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_83422_length_1735_cov_3.134524, whole genome shotgun sequence.
ACCESSION   DAOQYE010000506 DAOQYE010000000
VERSION     DAOQYE010000506.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83422_length_1735_cov_3.134524"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            322..570
                     /locus_tag="PKN52_08340"
     CDS             322..570
                     /locus_tag="PKN52_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L28 family ribosomal protein"
                     /protein_id="HNQ99997.1"
                     /translation="MPRVCAVTGKRTQSTTTSIRKGSAKRKGGVGLKQVGVHKRTLKP
                     NLQKRTVWVDGRPMRVWLSAKAIRQLDPTMFVNPNKPA"
     gene            639..1343
                     /locus_tag="PKN52_08345"
     CDS             639..1343
                     /locus_tag="PKN52_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HNQ99998.1"
                     /translation="MTVRFGDFEVLKGVSFDLPAGASLAVVGPNGAGKSTLVKVILGL
                     VAPLEGTVTVFGDRPGANPARVGYVPQLKTFDRTFPATALELVTTGLRRVWPGRVHSG
                     EREQALAALRRVGAEQLAARPLARLSGGELQRTYLARAFVRRPELVLLDEPATGVDYL
                     AERDIYDLLEEYQSDSGASVVMVTHDLTAARYHADRVLVLNRTARGFGLPEDVLSDEC
                     LEDAFGHRHHGHGPRV"
     gene            1435..>1735
                     /locus_tag="PKN52_08350"
     CDS             1435..>1735
                     /locus_tag="PKN52_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HNQ99999.1"
                     /translation="MSLTDALALPFFQRAVLAGLAIAVMSGVLGVFVVQRRLSFLGDG
                     LAHAAFGGMGIGAFVIVTTGLIGSSVALLKHPLWIALPFSVLAALDGRPDIAIGNV"
BASE COUNT          246 a          620 c          598 g          271 t
ORIGIN      
        1 gggtggggcg accgtaagaa cgagctggcg ttcatcggca tcgggatgga cgaggcggcc
       61 atccgcgcgt gtctcgacgc cgcgctcgtc accccgaccg aggcggcggc ggggttgacg
      121 tcctggtcgg tgttcaccga cccgttgccg gcctggggat gacggcccgt cggaccgcct
      181 cactgctccg ccccggtcgg gtctgtgcta accgaaggtc ctatgctaga ctcgattgct
      241 cgcaagcggg gcgcccaggt cgcgtcgcgc cgtcactcat gccctaccgg ggtcttcagc
      301 cccgtagcaa cggaggaagc aatgccgcgc gtctgtgcag taactgggaa gcgcacccag
      361 agcaccacca ccagcatccg caagggttcc gccaagcgca agggcggcgt cggcctcaag
      421 caggtcggcg tgcacaagcg cacgctgaag ccgaacctac agaagcgcac cgtgtgggtc
      481 gacggtcgcc ccatgcgcgt ctggttgagc gcgaaggcca tccgccagct cgacccgacg
      541 atgttcgtga acccgaacaa gccggcctga gagaggcacg catggcaggc gcggcaccgc
      601 tcaaccaggg cggtgccgtt tgcgttcgcc tgtcgggggt gacggtccgc ttcggcgact
      661 tcgaggtgct caagggcgtg tcgttcgacc tgcccgccgg cgcttcgctc gccgtggtcg
      721 gtccgaacgg cgcgggcaag tccaccctcg tcaaggtcat cctcggtctc gtcgccccgc
      781 tcgagggcac cgtgaccgtg ttcggtgacc ggccgggcgc caatccggca cgcgtcggct
      841 acgtgccgca gctcaagacg ttcgatcgca cgttccccgc caccgccctc gaactcgtga
      901 ccacgggcct gcgcagggta tggcccggcc gcgtgcactc gggcgagcgc gagcaggcgc
      961 tcgccgcctt gcggcgcgtc ggggccgagc aactcgccgc tcgaccgctg gcgcggctct
     1021 ccggcggcga gctgcagcgc acgtacctgg cccgggcgtt cgtgcggcga cccgagctcg
     1081 tgctactcga cgagcccgcc acgggcgtcg actacctggc ggagcgcgac atctacgacc
     1141 tcctcgagga gtaccagtcg gacagcggcg cgagcgtggt gatggtcacg cacgacctca
     1201 ccgccgcccg ctaccacgcc gaccgggtgc tggtcctcaa ccgcaccgcg cggggcttcg
     1261 gcctcccgga ggacgtgctg agtgacgaat gcctggagga cgccttcggg cataggcacc
     1321 acgggcacgg tccacgcgta tgagcgcgca tgactcctcg ccgtccgcgt cgtccggaga
     1381 ctgggcaagc tcgcggaccg ccgaccggcg ccccccgaca ggggtcagcg cggcatgagt
     1441 ctcaccgacg ccttggcgct gccgttcttc cagcgcgcgg tcctagccgg gcttgccatc
     1501 gccgtgatgt cgggcgtgct cggggtcttc gtcgtgcagc gccgcctgtc gttcctgggc
     1561 gacggcctgg cccacgccgc cttcggtggc atgggcatcg gcgcgttcgt gatcgtgacc
     1621 acgggcctaa tcggctcaag cgtcgcgctc ctgaagcatc cactatggat agcgttgcct
     1681 ttcagcgtcc tggcggcgct cgacggccgg cccgacattg ccatcggcaa tgtgg
//
LOCUS       DAOQYE010000507         1730 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_83884_length_1730_cov_2.233433, whole genome shotgun sequence.
ACCESSION   DAOQYE010000507 DAOQYE010000000
VERSION     DAOQYE010000507.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83884_length_1730_cov_2.233433"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(140..574)
                     /gene="rplK"
                     /locus_tag="PKN52_08355"
     CDS             complement(140..574)
                     /gene="rplK"
                     /locus_tag="PKN52_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HNR00001.1"
                     /translation="MAKKVAGIVKLQLPAGAATPAPPVGPALGQYGANIMQFVKEYNA
                     ATAGQAGAIVPVEITIYADRSFAFVTKTSPASYLIKKAAGIPKGSAAANKDKVGKLTW
                     AQCLEIGKTKMADLNASDEVAAAKIIAGTARSMGVTVEGQPS"
     gene            complement(722..1279)
                     /gene="nusG"
                     /locus_tag="PKN52_08360"
     CDS             complement(722..1279)
                     /gene="nusG"
                     /locus_tag="PKN52_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HNR00002.1"
                     /translation="MSIEWYAVHTYVGHEEKAKENILSRARSLGMAESIYQVVVPVET
                     TMEHIGGGKKKETTRKLFPGYIFVQMDLGDNPDEPNEAWEVVRYTPGVTGFVGTATHA
                     VPLSPDEEARMLTAIGVTGQREAPKVRITFNVGDMVQVTAGPFADFTGVVSEVNPERG
                     KVKVLVSIFGRETPVELDFSQVIRA"
     gene            complement(1347..1529)
                     /gene="secE"
                     /locus_tag="PKN52_08365"
     CDS             complement(1347..1529)
                     /gene="secE"
                     /locus_tag="PKN52_08365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HNR00003.1"
                     /translation="MFKGLVQYLRNSRAELGRVTWPTRKEVVQATEATLLFVLVTALF
                     LFVADLTLGNLLGLIT"
     gene            complement(1534..1698)
                     /gene="rpmG"
                     /locus_tag="PKN52_08370"
     CDS             complement(1534..1698)
                     /gene="rpmG"
                     /locus_tag="PKN52_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HNR00004.1"
                     /translation="MASDVRIKLLLECTECKRRNYATHKNRRNTQGKMELKMYCPWDR
                     KHTVHKEVKV"
BASE COUNT          256 a          554 c          569 g          351 t
ORIGIN      
        1 cggaagtgcg cctcgacggt ctcgtcgaac ttggcgctcg cgagctcctt gacgagcttg
       61 gccgcctcgt cgaccgagta cgtgcggtcc ttgtcgacct tcttggccaa ctcgcggtaa
      121 cgcttaccgt gctgcgccat cagcttggct gcccttcgac cgtgacgccc atggagcgcg
      181 ccgtgccggc gatgatcttg gccgcggcca cctcatcgga agcgttgagg tcggccatct
      241 tcgtcttgcc gatctcgagg cactgcgccc acgtgagctt gccgaccttg tccttgttgg
      301 cggcggcgga gcccttgggg atgccggccg ccttcttgat gaggtagctg gcaggcgagg
      361 tcttggtcac gaaggcgaag gagcggtcgg cgtagatggt gatctcgacc ggcacgatcg
      421 caccggcctg accagccgtc gcggcgttgt actccttcac gaactgcatg atgttggcac
      481 cgtactggcc cagcgccggc ccgacgggcg gcgcaggcgt ggccgctccg gccggcagct
      541 ggagcttaac gatcccggct actttcttgg ccatgcttcc tcctttggct cccccacgca
      601 catggcgagg cgcggggtgg cgcgccgcgt ctcgcggcgc attgcgctac tcaactactg
      661 cggcgctgct tgggcgccga tgtctacagc gcttggcggc ccggcaggac gccggcggcc
      721 gtcaagcgcg gatgacctgg gagaagtcca gttccacggg cgtctcgcgc ccgaagatcg
      781 agaccaggac cttgaccttg ccccgctccg ggttgacttc ggagacgacg ccggtgaagt
      841 cggcgaacgg accggcggtg acctggacca tgtcgccgac gttgaaggtg atcctgacct
      901 tgggggcctc gcgctggccg gtgacgccga tggcggtgag catgcgggct tcctcgtccg
      961 gggagagggg taccgcgtgg gtggcggtgc ccacgaagcc cgtcacccca ggtgtgtagc
     1021 gcacgacctc ccaagcctcg ttcggctcgt cagggttgtc ccccaagtcc atctggacga
     1081 agatgtagcc ggggaacagt ttgcgggtcg tctccttctt cttgccgccg ccgatgtgtt
     1141 ccatggtcgt ttcgacgggt acgacgacct gatagatcga ctcggccatg ccgagcgacc
     1201 gcgccctgct caggatgttc tccttggctt tctcttcatg ccccacgtag gtgtggacgg
     1261 cataccactc gatgctcata ggggttcctt tgtctggggc cgagctgcct aaggctctgg
     1321 cacccacggc gtgagacgac ctggggtcag gtgatcaggc cgagcaggtt gccaagcgtg
     1381 aggtcggcga cgaacaggaa cagcgcggtg acgaggacga acagcagcgt cgcctcggtg
     1441 gcctggacga cttccttgcg cgtcggccag gtcacgcggc cgagctcggc ccgcgagttc
     1501 ctcaggtact gaacgaggcc cttgaacacg agtctagacc ttcacctctt tgtgcaccgt
     1561 gtgcttgcgg tcccatgggc agtacatctt aagttccatc ttgccttggg tgttgcgccg
     1621 gttcttgtgc gtggcgtagt tgcgccgctt gcactccgtg cactctagaa gcagtttgat
     1681 ccgaacatca ctggccatcc tggcctccca atcagacctg acagtgtaac
//
LOCUS       DAOQYE010000508         1729 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_83980_length_1729_cov_1.847670, whole genome shotgun sequence.
ACCESSION   DAOQYE010000508 DAOQYE010000000
VERSION     DAOQYE010000508.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83980_length_1729_cov_1.847670"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="PKN52_08375"
     CDS             complement(<1..212)
                     /locus_tag="PKN52_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00005.1"
                     /translation="MKLAVTVGPPGSGRTERMLAAARAACDAGKRVWWVGLPAQRAHV
                     LHRLTANGYAALGFEFMSAQQLYYRLL"
     gene            complement(318..746)
                     /locus_tag="PKN52_08380"
     CDS             complement(318..746)
                     /locus_tag="PKN52_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00006.1"
                     /translation="MYDFVLTLHNYLRWALLLVGLAALVLAWSCAASRGRWTPPQVNL
                     GRLFTGLFDLQVLVGILLYAVLSPLTTGAFKNMGAAMKNPDLRFFVAEHLVGMLIAAV
                     LVHIGAARAKRKDSPLQAAIFYTLAFIVVIASIPWERPLF"
     gene            985..>1729
                     /locus_tag="PKN52_08385"
     CDS             985..>1729
                     /locus_tag="PKN52_08385"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNR00007.1"
                     /translation="MTKTASDRVAEAPTAGYDRLGEFHDLFVAAARRRLRPALAAAFG
                     GLRPDAVVLDLGAGTGLGVRQLAQATRARVVAVEPSVTMRAVLLARLADDPELTSRVT
                     VVAGSVPDAFDELPEEVAGFVCAHMLGHVSPADRALTFSALAARLAGGGVGVVTVNAT
                     AEPRSEAVVEERRIGVHRYIARYLPAGADGRPSSEYEVRDADGRLLRAERFPGHWTPI
                     TPEGLRAELVPAGWRVTTHDHAATVLNTAL"
BASE COUNT          246 a          592 c          654 g          237 t
ORIGIN      
        1 agcagccggt agtagagctg ctgggcgctc atgaactcga agccgagggc cgcgtaaccg
       61 ttggccgtga gccggtgcag gacgtgggcg cgctgcgcgg gcagccccac ccaccagacc
      121 cgcttgcctg cgtcgcacgc cgccctggcg gccgcgagca tgcgctccgt gcgacccgag
      181 ccgggtgggc cgaccgtgac ggctagcttc acccgggcat catacgagca gcctgcgacg
      241 cgtcctgacg cgcccccgac gcgctaggcg tggtggggag cgatgagccc gccggggccg
      301 ccgggtccga cttgcggcta gaagaggggg cgctcccagg ggatggaggc gatgacgacg
      361 atgaacgcaa gcgtgtagaa gatggcggcc tgcagcggag agtccttgcg cttcgcgcgc
      421 gccgccccga tatgcacgag caccgctgcg atcagcatgc cgaccaggtg ctccgcgacg
      481 aagaagcgca ggtcggggtt cttcatggcg gcgcccatgt tcttgaacgc gccggtcgtg
      541 agggggctca ggaccgcgta caggaggatg ccgacgagga cctgcaggtc gaagaggccc
      601 gtgaagagcc ggccgaggtt gacctgcggc ggcgtccagc gcccgcgcga ggccgcgcac
      661 gaccaggcga gcaccagggc ggccaggccg accaggagca acgcccagcg caggtagtta
      721 tgtagtgtca gtacgaagtc gtacatgagt tcctccgaga agtcctgtcg gacggcctgg
      781 cagcggcgcc cggcgcggtc tgcgacacgt gtgccacgct cgttcgcgct tctgcgatag
      841 aagggcgccg gccggcgacg gactcaagca tcggccaccc ttggccgggt gtcaactcgg
      901 gtgaggccat cggttacgct gcctgatcgt tcatcacggt ggcgtcacga cgctcccgga
      961 ggaccgggcg ttcgaggaga ccccatgacc aagaccgctt ccgaccgtgt tgccgaagcg
     1021 cctacggccg gctacgaccg gctgggcgag ttccacgacc tgttcgtcgc cgcggcccgc
     1081 cggcgccttc gtccggcgct ggcggccgcg ttcggtggcc ttcggccgga cgccgtcgtg
     1141 ctcgacctcg gtgccggcac ggggctcggc gtgcgccagc tcgctcaggc cacgcgggcg
     1201 cgggtcgtcg cggtggagcc gtcggtgacg atgcgggccg tgctcctcgc caggctagcg
     1261 gacgaccccg agttgacgag cagggtcacg gtcgtcgccg ggtccgtgcc cgacgcgttc
     1321 gacgagctgc cggaggaggt ggctgggttc gtgtgcgcgc acatgctcgg ccatgtgagc
     1381 cccgccgacc gcgcgctgac cttctccgcg ctggcggcgc gcctcgcggg cggcggggtc
     1441 ggggtcgtca ccgtgaacgc gacggcggag ccacggtccg aggcggtcgt ggaggagcgc
     1501 cggatcggcg tgcaccgtta catcgcccgc tacctgcccg ccggggcgga cgggcggccg
     1561 agcagcgagt acgaggtgcg cgacgccgac gggcggctac tgcgcgcgga gcgcttcccc
     1621 ggccactgga cgcccatcac cccagagggg ttgcgcgccg agctcgtccc tgccgggtgg
     1681 cgtgtcacca ctcacgacca cgcggcgacg gtgctcaaca cggcgctgg
//
LOCUS       DAOQYE010000509         1726 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_84185_length_1726_cov_2.501496, whole genome shotgun sequence.
ACCESSION   DAOQYE010000509 DAOQYE010000000
VERSION     DAOQYE010000509.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84185_length_1726_cov_2.501496"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            380..1033
                     /locus_tag="PKN52_08390"
     CDS             380..1033
                     /locus_tag="PKN52_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2847 family protein"
                     /protein_id="HNR00008.1"
                     /translation="MLKDRIVLLNSPEAVEAFFDRYPTSVVFKAGTCHKTMQGFGFVQ
                     EQLEGREDLMCGVIRVVEARPASNLVAERTGIQHESPQVIIFDSGKPVFAVDNWDITP
                     EALTEGFGRAQVGAPVAPPSIKAASDLRPYLDVLERYLSGTMDDSDFEHTYTYMFRGD
                     STLRPGDEVEALNSIFGDVDQHINMHLMMAGRADNSKLRQRAQAAYDRLREIAGATA"
     gene            complement(1037..1618)
                     /locus_tag="PKN52_08395"
     CDS             complement(1037..1618)
                     /locus_tag="PKN52_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00009.1"
                     /translation="MNSLTVLVLLLFIGVVALALTAWSALAVLRRRRVEPSDEPREVR
                     AGEHAHVAQPRPRTEAPVAKRVVVAPPRVAAEPKPHPATPNVTDQPRATVEPAPYLSK
                     PPTEIKPGRSGRTIREPAVAPVGPSVGETGQKAGGTSVRPRVVVTESEQPRAQQQVEQ
                     NAPANETPRVTVKPREVEPNAFDRFIDAERRRN"
BASE COUNT          269 a          589 c          577 g          291 t
ORIGIN      
        1 aaaccactcg gacacctctc cgcgacgctt cggaccctga agtcgggaat ccgaaccgtg
       61 ttgcgcgtcg ataggcttgc ctaccggccg cgacggcaac acggagtgta acaggcgccc
      121 ctgggcccga caagtccgtg tatgcgttca ggtcctatgg ccgctgcgct ctgacgcgac
      181 cggcacccgg ccctgtagcg gtcgctcgca gcgggtcatg ccgacgatct agcaaggcat
      241 gggcgccttt ccatgggttc ctggaagact gccactcccg ccgcgctgcg gatacactct
      301 tcaccagttg tgctcaccag ctgtgttcac agttgtgttc accagttgta acggatcaag
      361 agttgtaacg gaggctgcaa tgctcaaaga ccggatcgtt ctcctcaact cgcccgaggc
      421 cgtcgaggcc ttcttcgacc gctaccccac cagcgtcgtg ttcaaggccg gcacctgcca
      481 caagaccatg cagggcttcg gcttcgtgca ggagcagctc gaggggcgcg aggacctgat
      541 gtgcggcgtc atccgcgtcg tcgaggcgcg tccggccagc aacctcgtcg ccgagcgtac
      601 gggcatccag cacgagtcgc cgcaggtcat catcttcgac tccggcaagc ccgtcttcgc
      661 cgtcgacaac tgggacatca cgccggaggc gctgaccgag ggcttcggtc gcgcccaggt
      721 aggggcgccc gtggcgccgc ccagcatcaa ggccgcctcc gacctgcgcc cctacctaga
      781 cgtgctcgag cgttacctct ccggtaccat ggacgactcg gacttcgagc acacctacac
      841 gtacatgttc cgcggcgact cgacgctccg gcctggcgac gaggtcgagg cgctcaactc
      901 gatcttcggc gacgtcgacc agcacatcaa catgcacctg atgatggccg gcagggccga
      961 caactccaag ctgcgccagc gggcgcaggc ggcctacgac aggcttaggg agatcgcggg
     1021 cgctacggcc tgaggctcag ttacgccggc gttcggcgtc gatgaaacgg tcgaacgcgt
     1081 tgggctccac ctcgcgtggc ttcacggtca cgcgaggtgt ctcgttggcg ggcgcgttct
     1141 gttctacctg ctgctgggct ctgggctgct cggactcggt caccacgacg cgcgggcgca
     1201 cgctggtgcc gccggccttc tgacctgtct cacccaccga tgggccgacc ggcgctaccg
     1261 ccggttcgcg gatcgtcctg ccgctccgtc cgggcttgat ctcggtcggg ggtttggaca
     1321 ggtagggcgc cggctccacc gtcgctcggg gctggtctgt gacgttgggc gtagcagggt
     1381 gaggcttcgg ctcggccgcg acgcgcggcg gcgctaccac gacgcgcttc gcgacgggcg
     1441 cttcggtccg agggcgcggc tgggccacgt gagcgtgctc gccggcgcgc acctcgcgcg
     1501 gttcgtcgga cggctcgacg cggcgccggc gcaacacggc gagcgccgac caggccgtca
     1561 gggcgagcgc gacgacgccg atgaagagca ggagcaccag gacggtgagc gagttcacta
     1621 catgaggata gtctgccgca gacttgctgg caacgcgttt gggccgcgcg ccaccctacg
     1681 tcaggtggtt tagcggaccc ccagcagcgc gcagagcttg gcgggg
//
LOCUS       DAOQYE010000510         1724 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_84347_length_1724_cov_1.910126, whole genome shotgun sequence.
ACCESSION   DAOQYE010000510 DAOQYE010000000
VERSION     DAOQYE010000510.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1724
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84347_length_1724_cov_1.910126"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..967
                     /locus_tag="PKN52_08400"
     CDS             <1..967
                     /locus_tag="PKN52_08400"
                     /inference="COORDINATES: protein motif:HMM:NF033681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ExeM/NucH family extracellular endonuclease"
                     /protein_id="HNR00010.1"
                     /translation="QSDERGHDLGPLPPQFLSRVGADTGEELRRQRAKIVAALVRLDA
                     HVLGLTELENDPAGNALADLVSALNSATSADHYAAVRTSGPVGGDVIRVAILYQPALV
                     RPVAVTAVLDDRSFIDPTRSGEARNRAAVAQTFEEVASGERFTVVVNHLKSKSSTCGR
                     GDDDAVQGSCNGTRTAAVKGLLAWLATDPTGAGDPDVLLLGDYNAYAREDPVRALGAG
                     QDGRTGNADDFTDLLGKFVGEGAYTYVYDAQLGYLDYAFASGTLLPQVTGATAWHINA
                     DEPDILDYDLTFKPSAQAGLFTADPYRSSDHDPVLVGLALGADGR"
     gene            1044..>1724
                     /locus_tag="PKN52_08405"
     CDS             1044..>1724
                     /locus_tag="PKN52_08405"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HNR00011.1"
                     /translation="MDEFAQLTSKRRSSNQTSNRLVLIGIVVVLAAILVFASMFTVDQ
                     GYRGVVLRLGAFERIASPGLGFKLPLVDRVVMLRVQTNSKVYDRMETYSRDQQLAELR
                     VSVTYRLVPEMVHEVYAQFGSEDGVVSRLIDRRVNELVRTVFGQFNAVEAIQQRSSLN
                     QKVADAVMSVIDPNIVVIESVQVEDIAFSSAYEQSVEARMMAEVEVQRRTQELEQQRI
                     QAQIVVTQA"
BASE COUNT          300 a          593 c          564 g          267 t
ORIGIN      
        1 ccagtctgac gagcgcggcc acgatcttgg ccctttgccg ccgcagttcc tctcacgtgt
       61 cggcgccgac acgggagagg aactgcggcg gcaaagggcc aagatcgtgg ccgcgctcgt
      121 cagactggac gcgcacgtgc ttggtctcac cgagctggag aacgacccgg cgggcaacgc
      181 gctcgccgac ctcgtgagcg ccctcaactc cgcgacgtct gccgaccact acgccgcggt
      241 gcggaccagc ggacccgtgg gcggcgacgt cattcgcgtc gcgatcctct accagccggc
      301 gctcgtgcgc ccggtcgccg ttaccgccgt gctggacgat aggagcttca tcgacccgac
      361 gcgctccgga gaggcgcgca accgcgccgc cgtcgcacag acgttcgagg aggtcgcaag
      421 cggcgagcgc ttcacggtgg tggtcaacca cctgaagagc aagagctcga cctgcggccg
      481 cggcgatgac gatgccgtcc agggcagctg caacggcacg cgcacggcgg cggtcaaggg
      541 cctgctggca tggttggcca cggaccccac cggcgccggc gaccccgacg tcctgctcct
      601 cggcgactac aacgcgtacg cgcgggagga cccggtgcgc gcgctcggtg ccggtcaaga
      661 cggccgtacc ggcaacgcgg acgatttcac ggacctgctc ggcaagttcg tcggcgaggg
      721 cgcgtacacc tacgtctacg acgcgcaact cgggtacctc gactacgcct tcgccagtgg
      781 tacgttgctc ccgcaggtga caggcgcgac cgcctggcac atcaacgccg acgagcccga
      841 catcctcgat tacgatctga ccttcaagcc cagtgctcaa gcggggctgt tcacggccga
      901 cccgtatagg tcgtccgacc acgacccggt gctagtcggt ttggcgctgg gcgcggacgg
      961 tcgttgacgg agcacgtcaa gcacggcccc tcgaagcgcc tgcaacgaac agactgcccc
     1021 ggtacggtac catcgacatt accgtggacg aattcgccca gctaacgagc aagcgcaggt
     1081 catccaacca gacttcgaac cgcctggtcc tcataggcat cgtcgtcgta ctggcagcca
     1141 tcctggtgtt cgccagcatg ttcaccgtcg accagggcta ccgcggcgtc gtgctgcgcc
     1201 tcggcgcgtt cgagcgcatc gcatcgcccg gcctcggttt caagctaccc ctcgtcgaca
     1261 gggtcgtgat gctgcgagtg cagaccaact cgaaggtcta cgaccgtatg gagacgtact
     1321 cccgcgacca gcagctcgcc gagctgcgcg tgtcggtcac gtaccggctc gtgccggaga
     1381 tggtgcacga ggtctacgct cagttcggca gcgaggacgg cgtcgtgtcg cgcctcatcg
     1441 accggcgcgt caacgagctg gtgcggaccg tgttcggcca gttcaacgcc gtcgaggcca
     1501 tccagcagcg gagctcgctc aaccagaagg tggccgacgc cgtgatgagc gtcatagatc
     1561 cgaacatcgt cgtgatcgag tccgtccagg tcgaggacat cgcgttcagt tcggcatacg
     1621 agcagtcggt cgaggcgcgc atgatggccg aggtcgaggt gcagcgccgc acgcaggagc
     1681 tcgagcagca gcgcatccag gcgcagatcg tcgtcactca ggcg
//
LOCUS       DAOQYE010000511         1721 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_84521_length_1721_cov_2.974190, whole genome shotgun sequence.
ACCESSION   DAOQYE010000511 DAOQYE010000000
VERSION     DAOQYE010000511.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84521_length_1721_cov_2.974190"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1340
                     /locus_tag="PKN52_08410"
     CDS             <1..1340
                     /locus_tag="PKN52_08410"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HNR00012.1"
                     /translation="HYVQERFGTWSWEQVLAPAIKVAEEGFVADEIYTRRMNATRALL
                     LEHGDPALIEPFEATFFPDGRELRPGELIVQRDMAKTMRRLAAEGPGLFYGGDVSRQI
                     VSWVAEHGGILSDVDFTSYKVESGPLSVTTYRGYEIHGYANCANGSTIAAIMNILESF
                     DLRSMGYLSADLIHVVAEAMKLAFSDRYAYVSDRDQVPVPYDGLMSKSYARERAKLID
                     PQRAKPHRPGNPWRHQASGAKLYEEFGVDLAGLTGGGTAKEPDSDTTFMVAGDSEGNL
                     MLITSSNFSNCKVVVPGLGFQLNNSMEGANPAPGHALSIAPFKRILRNSGPTLVFKDG
                     EPVYALSAPGGRRIITGTVWALIQMIDFGLGPQAALDAPRMHCEARGMQVRIEDGVGE
                     ATLDELRARGHELDVSPMYQEVYSRMQALKIDRTAGLYVGGSEPRSHSGVRGY"
     gene            1343..>1721
                     /locus_tag="PKN52_08415"
     CDS             1343..>1721
                     /locus_tag="PKN52_08415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HNR00013.1"
                     /translation="MITLALAEYISSALSKPLPTAVVLKAKHHVLDTIAAMLSGTQLL
                     PGERALAYARRLGGPAEAFVLGSVIGGMYLCAPSSFEAALCLNWGLTTDVVSGTWSIK
                     RTTKVVSVTPETGYANTVNDPEMD"
BASE COUNT          302 a          573 c          579 g          267 t
ORIGIN      
        1 accactacgt gcaggagcgc ttcgggacgt ggtcgtggga acaggtgctc gcccccgcca
       61 tcaaggtcgc cgaggagggg ttcgtcgccg acgagatcta cacgcggcgc atgaacgcta
      121 cccgggcgct gctgctcgag cacggcgacc ccgccctgat cgaaccgttc gaggccacgt
      181 tcttcccgga cggccgggag ttgcgcccgg gcgaactgat cgtgcagcgc gacatggcga
      241 agacgatgcg ccgcctcgcg gcggaaggcc cggggctctt ctacggcggc gacgtatcgc
      301 ggcagatcgt ctcctgggtc gccgagcacg gcgggatcct cagcgacgtc gacttcacgt
      361 cgtacaaggt cgagtccgga cccttgagcg tgacgaccta ccgaggctac gagatccacg
      421 gctacgccaa ctgcgcgaac ggctccacca tcgccgcgat catgaacatc ctcgagagct
      481 tcgacctgag gtcgatgggc tacctgagcg cggacctgat acacgtcgtt gcggaagcca
      541 tgaagctggc gttctccgat cgctacgcgt acgtgagcga ccgcgaccag gtgccggtgc
      601 cgtacgacgg actgatgtcc aagagctacg cgcgcgagcg ggcgaagctg atcgacccgc
      661 aacgcgcgaa gccgcatagg ccgggcaacc cgtggcggca ccaagccagc ggggccaagc
      721 tctacgagga gttcggcgtc gacctcgcgg gcctcacggg cggcggcacc gccaaggagc
      781 ccgactccga caccacgttc atggtggcgg gcgactccga gggcaacctc atgctcataa
      841 cctcctccaa cttctccaac tgcaaggtag tggttccggg cctcgggttc cagctcaaca
      901 actcgatgga gggggccaac ccggcgcctg ggcatgcgct gtcgatcgcg ccgttcaaac
      961 gcatcctgcg taacagcggg ccgacgctcg tgttcaagga cggcgagccc gtctacgcgc
     1021 tctcggcgcc gggtggccgg cggatcatca caggcaccgt ctgggcgctc atccagatga
     1081 tcgacttcgg cctcgggcca caagcggctc tcgacgctcc gcgtatgcac tgcgaggcgc
     1141 gcggcatgca agtgaggatc gaggacgggg tcggggaggc gacgctcgac gaactgcgcg
     1201 cccgcgggca cgagctcgac gtgtcgccca tgtaccagga ggtctactcc cgcatgcagg
     1261 cgctcaagat cgaccgcacc gccgggctgt acgtaggcgg gtcggaaccg aggagccaca
     1321 gcggggtcag aggctactga tggtgatcac gctcgccctg gccgagtaca tctcatcggc
     1381 gctgagcaag cccctgccga ccgccgtggt gctgaaggcg aagcaccacg tgctcgacac
     1441 catcgccgcc atgctctcgg gtacgcaact acttcccggc gagcgggcac tggcctacgc
     1501 ccggcgcctt ggcgggccgg ccgaggcgtt cgtcctcggc agcgtgatcg gtggcatgta
     1561 tctgtgcgcg ccgtcttctt tcgaagctgc gctgtgcttg aactggggcc tgacgacgga
     1621 tgtcgtttcg ggcacctggt cgatcaagcg caccaccaag gtggtgtcgg tcactcctga
     1681 gacggggtac gcgaacacgg tcaatgaccc ggagatggac g
//
LOCUS       DAOQYE010000512         1715 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_84984_length_1715_cov_3.007831, whole genome shotgun sequence.
ACCESSION   DAOQYE010000512 DAOQYE010000000
VERSION     DAOQYE010000512.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84984_length_1715_cov_3.007831"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..640)
                     /locus_tag="PKN52_08420"
     CDS             complement(<1..640)
                     /locus_tag="PKN52_08420"
                     /inference="COORDINATES: protein motif:HMM:NF024248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNR00014.1"
                     /translation="MNQTDTRRIPTNRHLSEARWRLLGEVLRHRDRGQTVEELSEALS
                     VSRNAVQQHVTSLERDGLLRVLGHRMTGGRPGRAYTLTEAALELFPRSYAKLADDMLR
                     NVRELFGESGLDRMLVKMADEVASGLEPRLAGKKGAARIAEVAAILHELGYEATVDDG
                     GRLRAANCVFHQLARSSAAVCRYDQVLLRRLLGDDVAQLACITDGSAACVFAL"
     gene            771..1358
                     /locus_tag="PKN52_08425"
     CDS             771..1358
                     /locus_tag="PKN52_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00015.1"
                     /translation="MTRTIATQSEAPAAGARATPSPSARPFAARSRNLVLGFVALVLM
                     TAACAPAATGSSSTPIVVGSSAIRVEQGRTYYLRFDHSLAQFGLAPKDLQVNLWVPSG
                     YNASRGDAASTFGLLDARVADRWQVALSSVMVEQRNTTSASFNTTTTDYSLWSVVKVV
                     VPDDIIPGPYRLRATLQARGGKSVPLAATLDVQPK"
     gene            complement(1368..>1715)
                     /locus_tag="PKN52_08430"
     CDS             complement(1368..>1715)
                     /locus_tag="PKN52_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00016.1"
                     /translation="DDATALLAELAAAVGGGMTAAASFYARASDRMRGALGGEAHFAR
                     AFTNDRFAPLAYGSGARLTDIQQLGDSVRATLVIETETGTVAYVVAVAKARFGDRAGT
                     WCLSGVAREGIDL"
BASE COUNT          245 a          633 c          571 g          266 t
ORIGIN      
        1 tgagcgcgaa cacgcaagcc gccgagccgt ccgttatgca cgccagttga gccacgtcgt
       61 cgccgagcaa gcgcctgagc agcacctggt cgtaccggca taccgccgcg ctggagcgcg
      121 ccagctgatg gaacacgcag ttggcggctc gcagccgacc gccgtcatcg acggtcgcct
      181 cgtaaccgag ctcgtgcagg atggccgcga cctcggcgat ccgcgcggcg cccttcttgc
      241 cggccagccg tggctccagc ccgctcgcga cctcgtcggc catcttgacg agcatccggt
      301 caagcccgct ctcgccgaac agttcgcgca cgttgcgcag catgtcgtcc gcgagcttcg
      361 cgtagctcct cgggaacagc tccagcgcgg cctcggtcag cgtgtaggca cgcccaggcc
      421 ggccgcccgt catgcggtgg ccgagcacgc ggagcaggcc gtcgcgctca agggacgtga
      481 cgtgctgctg gacggcgttg cgtgacacgg acagcgcctc ggacagctcc tcgacggtct
      541 gaccgcgatc gcggtggcgc aggacctcgc cgaggagacg ccaccgcgcc tcggagaggt
      601 ggcggttcgt cgggatcctg cgagtgtcgg tctggttcat cgtctggtgg gccacgtcaa
      661 atgccgcgcc gcccgcgcct tcccgggcat catggcatgg gcgcttgccc tcatgtcgcc
      721 gcggtgtacg taccctaccg gccctcatgc gcctctgata gcctcgaacc atgacgcgaa
      781 ccatcgctac ccaatcggag gcccccgcgg ccggcgcgcg ggctacgccc tcgccctcgg
      841 ctcgcccgtt cgcggcccgt tcgcgcaacc tagtgcttgg cttcgtcgcg ctcgtgctga
      901 tgacggcagc gtgcgccccg gcggccacgg gctcgagctc caccccgatc gtcgtcggta
      961 gctcggccat ccgggtcgag cagggccgga cctactactt gcgcttcgac cactcactgg
     1021 cccagttcgg cttggcgccg aaggacctcc aagtgaacct gtgggtgccg tccggttaca
     1081 acgcgtcgcg cggcgacgcc gccagcacgt tcggtctcct cgacgcccgc gtcgcggacc
     1141 gatggcaggt cgcgctgagt agcgtgatgg tcgagcagcg caacacgacc agcgcgtcgt
     1201 tcaacaccac cacgaccgac tactcgctgt ggtccgtcgt gaaggtcgtg gtgcccgacg
     1261 acatcatccc aggcccctac aggctgcgtg ccacgttgca ggcgcgcggc ggcaagagcg
     1321 tcccgttggc cgcgacgctg gacgtgcagc ccaagtagcg cgcaaggcta caggtcgatg
     1381 ccctcgcggg ccacgccgct gaggcaccat gtgccggcgc ggtcgccgaa acgcgccttc
     1441 gccacggcta cgacgtaggc caccgtgccg gtttccgtct cgatcaccag cgtcgccctg
     1501 acgctgtcgc ccagttgctg gatgtcggtc aggcgggcgc cgctcccgta ggcgaggggc
     1561 gcgaagcggt cgttagtgaa agcacgcgcg aagtgggcct cgccaccgag ggctccacgc
     1621 atgcggtcgg acgcgcgcgc gtagaagctg gctgcggccg tcatgccccc gcccaccgcc
     1681 gccgccagct ctgctagcaa cgccgtggcg tcgtc
//
LOCUS       DAOQYE010000513         1715 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_85001_length_1715_cov_2.559036, whole genome shotgun sequence.
ACCESSION   DAOQYE010000513 DAOQYE010000000
VERSION     DAOQYE010000513.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85001_length_1715_cov_2.559036"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..916)
                     /locus_tag="PKN52_08435"
     CDS             complement(<1..916)
                     /locus_tag="PKN52_08435"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNR00017.1"
                     /translation="MLLAAIHSVDKAYGEQVVLDAATLELRDGDRTALIGRNGAGKST
                     LLRLLMGAEAPDAGSVYRAEGTVIAMLEQDPRFEPGETIVGISERAFVELDELERRLA
                     ALEAAGLDDPDRYHLWETVHATFERRGGYARRARRDAVLSALGFKERHHELVASLSGG
                     ERTRLGLARLLMAQPDVLLLDEPTNHLDIEMRAWLEGHLARYPGAALIVSHDRAFLDG
                     ACDRTAEVSRGELRVGAGNPTAFRAYWAEAERIQAQTRANQERELARLDAAAEQMKRW
                     AGQSEKLHRRAKAMERRADRYETDMIDEV"
     gene            complement(1047..>1715)
                     /locus_tag="PKN52_08440"
     CDS             complement(1047..>1715)
                     /locus_tag="PKN52_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HNR00018.1"
                     /translation="LGRAIVREPKVFLMDEPLSNLDAKLRVEMRASITKLHQRLGVTT
                     VYVTHDQVEAMTMGTRIVVMKDGYIQQVDSPIHLYDRPVNKFVAGFIGSPAMNFMVGT
                     VQDGRLKSPQFDLKPNGELAAKLAAYNGKKVYVGIRPEAIDLKGFTDIPEGDNVIRAT
                     VDVVEPLGAETHIIASVGGDQSVVARVDAHANVKPGDSVELLARLDSLHAFDMETEKN
                     IRFE"
BASE COUNT          224 a          562 c          623 g          306 t
ORIGIN      
        1 tcacctcgtc gatcatgtcg gtttcgtagc ggtccgctcg gcgctccatg gccttggcgc
       61 gccggtggag cttctcgctc tggccggccc agcgcttcat ctgctccgcg gcggcgtcca
      121 ggcgggccag ctcgcgctcc tggttggcgc gcgtctgcgc ctggatgcgc tcggcctcgg
      181 cccagtaggc gcggaacgcg gtcgggttgc cggcgccgac gcgcagctcg ccgcggctga
      241 cctcggccgt gcggtcgcag gcgccgtcga ggaaggcgcg gtcgtgcgag acgatgagcg
      301 cggcgcccgg gtagcgtgcc aggtggcctt ccagccaggc gcgcatctcg atgtcgaggt
      361 ggttggtcgg ctcgtctagg agcagcacgt cgggctgcgc catgagcagc cgcgcgaggc
      421 cgagcctggt gcgctcgccg ccggacaggc tggcgacgag ctcgtggtgc cgctccttga
      481 acccgagcgc gctcagcacg gcgtcgcgcc gggcgcgccg cgcgtagccg ccccgccgct
      541 cgaaggtcgc gtggaccgtc tcccataggt ggtagcggtc cgggtcgtcc aggcctgcgg
      601 cctccagggc cgcgaggcgt ctctccaact cgtcgagttc gacgaacgcc cgctcgctga
      661 tgcccacgat ggtctcgccc ggctcgaagc gcgggtcctg ctccagcatg gcgatgacag
      721 tgccttcggc ccggtagacg ctgccggcgt cgggggcctc cgcccccatg agcaggcgca
      781 gcagcgtgct cttgccggcc ccgttgcggc cgatgagggc ggtgcggtcg ccgtcgcgca
      841 actccagcgt ggcggcgtcg agcacgacct gctcgccgta cgccttgtcg actgaatgga
      901 tggcggccag gagcacgacg gtagtctacc gttgccactc ctggccgcta agtcttgcta
      961 gggcgccacc cggtaagtct tgctaatcac cgggcatgta ctgcttaagt cacccggcat
     1021 gtactgctta agcgccgcgt tggcgctcac tcgaagcgga tgttcttctc ggtctccatg
     1081 tcgaaggcgt gcaacgagtc gagccgcgcg aggagctcga cggagtcgcc gggcttgacg
     1141 ttcgcgtgcg cgtcgacgcg cgcgacgacg ctctggtcgc cgccgaccga ggcgatgatg
     1201 tgcgtctcgg caccgagggg ctcgacgacg tcgaccgtgg cgcggatgac gttgtcgcct
     1261 tcggggatgt cggtgaagcc cttgaggtcg atggcctcgg gccggatgcc gacgtagacc
     1321 ttcttgccgt tgtacgcggc gagcttggcg gcgagttcgc cgttgggctt gaggtcgaac
     1381 tgcgggctct tgaggcggcc gtcctggacg gtgccaacca tgaagttcat ggcaggcgag
     1441 ccgatgaacc cggcgacgaa cttgttgacc ggacgatcgt acagatggat ggggctgtcg
     1501 acctgctgga tgtagccgtc cttcatgacc acgatgcgcg tgcccatcgt catggcctcg
     1561 acctggtcgt gggtcacgta gacggtcgtg acaccgaggc gctggtggag cttggtgatg
     1621 ctggcgcgca tctcgacacg cagcttggcg tcgaggttcg agagcggctc gtccatgagg
     1681 aagaccttcg gctcgcgcac gatggcgcgg ccaag
//
LOCUS       DAOQYE010000514         1714 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_85094_length_1714_cov_2.242917, whole genome shotgun sequence.
ACCESSION   DAOQYE010000514 DAOQYE010000000
VERSION     DAOQYE010000514.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1714
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85094_length_1714_cov_2.242917"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(155..1480)
                     /locus_tag="PKN52_08445"
     CDS             complement(155..1480)
                     /locus_tag="PKN52_08445"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HNR00019.1"
                     /translation="MPPDESQQAQEQLVQSTDAPNPGPSNAPGEPVLDLVLVHHQPGV
                     GAGLRKVRAFTWLLLALLALTAVLGSQLRPEPRLAHVAGTPPVIEAPTGALLTAYENT
                     RDATVRIEARCIANARAVLGVGTGFFVSEDGMVLTAYHVVSAASEGQCRAKLVAVTTD
                     RLEFDLHLVGFDAYMDLAALQADVPSLVPYIPLATRLPSPGTGVVAIGNSRNDFMGAR
                     AGRVTRLGVRAGRADFADDTIELTNSLAPGDSGGPVVNARGEAVGVVSYISFNPNAMS
                     SQGFIPPFLQGLAINRDFAGYAIPLTRTSELVEGVLAGGKRDVPVIGFSWAQGLDYDP
                     RTSQHYLGARPGPIVSEVAPGGPAAKAGLRSLSQESVVATDGTVSVTPVADVIVAIDG
                     TPTPTFSDLLAVVRGKNIGDVVTLTVQRGNATFRVELTLGARTSVFAGN"
     gene            1528..>1714
                     /locus_tag="PKN52_08450"
     CDS             1528..>1714
                     /locus_tag="PKN52_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00020.1"
                     /translation="MSPFPDNRGDPAYRLVLAEMADRARAWPGPVVVTAHVDPDGDAL
                     GSCLTLARALRALGKRVT"
BASE COUNT          259 a          593 c          610 g          252 t
ORIGIN      
        1 caccttcggt cgcctaaacg ccgttgtcga gtccactatc gcccaccaga agcagaagga
       61 acacgacaac ggcgtttagg cgaccgaagg tggcctaaac gccgttgtcg cgtcttcgac
      121 acgcccctcc gccgagcggg aacgggcgcg gtcgtcagtt gccggcgaac accgaggtgc
      181 gcgcgccgag cgtcaactcg accctgaacg tggcgttgcc gcgctgaacc gtcaaggtga
      241 ccacgtcgcc gatgttcttc ccccgcacca cggccagcag gtcggagaac gtgggcgtcg
      301 gcgtgccgtc gatcgcgacg atcacgtccg ccaccggcgt cacgctcacc gtgccgtcgg
      361 tcgccacgac gctctcctgg ctgagcgacc tgaggccggc cttggcggca ggcccgccag
      421 gggcgacctc gctgacgatc gggcccggcc gtgcgccgag gtagtgctgg ctggtgcgcg
      481 ggtcgtagtc gagcccttgc gcccacgaga acccgatgac cggcacgtcg cgtttgcccc
      541 cggcgagcac cccctcgacc agctcgctcg tgcgcgtcag cgggatggcg taacccgcga
      601 aatcccggtt gatggccagc ccttggagga agggtgggat gaagccctgc gagctcatgg
      661 cgttcgggtt gaagctgatg tagctcacga cgccaacggc ctcgccgcgc gcgttgacca
      721 cgggtccgcc cgagtcgccc ggcgcgaggg agttggtgag ctcgatcgtg tcatcggcga
      781 agtcggcgcg acccgccctg acgccgaggc gcgtcacgcg tccggccctg gcgcccatga
      841 agtcgttgcg gctgttgccg atggccacga ccccggtgcc ggggctgggc agccgggtgg
      901 cgagcgggat gtaagggacg aggctcggta cgtccgcctg cagcgcggcc aggtccatgt
      961 aagcgtcgaa cccgacgagg tgcaggtcga actcgaggcg gtcggtcgtc actgcgacga
     1021 gcttggcgcg gcactggccc tcggaggcgg cggagaccac gtggtaggcc gtgaggacca
     1081 tgccgtcctc ggacacgaag aagccggtgc cgacaccgag cacggcgcgc gcgttggcga
     1141 tgcaccgcgc ctcgatgcga acggtggcgt cgcgcgtgtt ctcgtaagcc gtgaggagcg
     1201 cgccggtggg cgcctcgatg acgggcgggg tgccggccac gtgcgcgagg cgcggctcag
     1261 gcctgagttg gctgccgagc acagcggtga gcgccagcaa cgcaaggagc agccacgtga
     1321 acgcccttac cttgcgaagg ccggccccca caccgggttg atggtgcacg agcacgaggt
     1381 cgagcaccgg ttcaccgggc gcgttagaag gcccggggtt cggtgcgtcg gttgactgga
     1441 ccaactgctc ctgagcctgc tggctctcgt ctggcggcat acgaccatta tgcggacgga
     1501 aacgctcccg tgcgtatcat ggctctcatg tcgcctttcc cagacaaccg cggcgacccg
     1561 gcgtaccgcc tggtcctcgc ggagatggcc gaccgcgcac gcgcctggcc cggccccgtc
     1621 gtcgtcaccg cccacgtcga ccccgacggg gacgcgctcg gtagctgcct caccctcgcc
     1681 cgggcgctgc gcgcgctcgg caagcgggtc accg
//
LOCUS       DAOQYE010000515         1713 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_85171_length_1713_cov_2.358263, whole genome shotgun sequence.
ACCESSION   DAOQYE010000515 DAOQYE010000000
VERSION     DAOQYE010000515.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85171_length_1713_cov_2.358263"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1099)
                     /locus_tag="PKN52_08455"
     CDS             complement(<1..1099)
                     /locus_tag="PKN52_08455"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNR00021.1"
                     /translation="MLLSSLSSPALAQEEGSAASASFAAAYEQAEQAFVTALRTDLGR
                     APSLDDAVWHDAVTVAEALPDAAADPDDRLSANRFLAHVYGATNWYIRAFAAWDAFLA
                     DGGELSGQRLPLPADVSDKLLPDDAEFHRVVGQLAFARYEAGDPGGARSWYLTLLDMV
                     PDDPEALRWLARIAFEENDTSTAVAIWQRLVEVAPEDEGAKFFLELSQERARFGEEAS
                     EAYRRGLRAYEAGDSTEAFDDFRAAVGSNPEFTDATVWAGRAALDLDLPAQAVGYWER
                     AVTLDPEDARSRWFLEYARTLDRWGVAAGRAYYAGLAAYEAGELDAAAAEFLAAAEAA
                     PEFVDAYVWAARSTQEAGRPAEAIAYWQAVLR"
     gene            complement(1165..>1713)
                     /locus_tag="PKN52_08460"
     CDS             complement(1165..>1713)
                     /locus_tag="PKN52_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00022.1"
                     /translation="AAGLFPNAGRRRVPGWGGRRSSVGDRAKRLARSALTACVLSGAA
                     LLGASAHAQLLADVLMTAAISAQFDSGVSLPRGSMRAVGDGVGALVARLPDSPKWTDW
                     EAYVLGGVAVGLAPGVVHEIATAYAVAGYFETERSEQSVAGPQGPETHTRIVFVGDDG
                     SSRLLYVIRADREVVWLTARAR"
BASE COUNT          270 a          634 c          597 g          212 t
ORIGIN      
        1 gccgcaacac ggcctgccaa taggcgatgg cctcagccgg acgaccggcc tcctgggtgc
       61 tgcgcgcggc ccagacgtag gcgtcgacga actccggcgc cgcctcggcg gctgcgagga
      121 actccgcggc cgcggcgtcg agttcccctg cctcgtaggc agcgagcccg gcgtagtaag
      181 cgcggccggc tgccacgccc catcggtcga gggtccgggc gtactccagg aaccaccgcg
      241 accgggcgtc ctcggggtcg agggtcaccg cccgctccca atacccgacg gcctgagcag
      301 gcaggtccag gtccagcgcc gcgcggccag cccacacggt cgcgtcggtg aactccgggt
      361 tgctgccgac ggccgctcga aagtcgtcga acgcctcggt ggagtcgccg gcctcgtaag
      421 ccctcaaccc gcgccggtag gcctcgctgg cctcctcacc gaagcgcgcc cgctcctgac
      481 tcaactcgag gaagaacttg gcgccctcgt cctccggtgc gacctcgacg aggcgctgcc
      541 agatcgcgac ggccgtcgac gtgtcgttct cctcgaacgc gatgcgagcg agccacctga
      601 gcgcctcggg atcgtcgggc accatgtcga ggagcgtgag gtaccagctc ctcgcgccac
      661 ccgggtcgcc ggcctcgtag cgcgcgaagg caagctgacc gaccacccgg tggaactcgg
      721 cgtcgtcggg caagagcttg tcggagacgt ccgcgggtag agggaggcgc tggcccgaca
      781 gctctccccc gtcggccaag aaggcgtccc acgcggcgaa cgccctgatg taccagttgg
      841 tcgccccgta gacgtgcgcg aggaagcggt tggcgctcag tcggtcgtcc gggtcggcgg
      901 cggcatccgg aagcgcctcg gcgaccgtaa cggcgtcgtg ccatacggca tcgtcaaggc
      961 tgggcgcgcg gccgaggtcg gtgcgcaggg cggtcacgaa cgcctgctcg gcctgctcgt
     1021 aagcggcggc gaacgacgcg ctcgcagcgg agccctcctc ctgagcgagc gccggggaag
     1081 agagcgacga aaggagcacg acaagggcgg gcagccacct ccgccctcca ctacgccagg
     1141 ggtcccgacg gcaggcgaca ggcctcagcg cgctctcgcc gtcaaccaca cgacttcccg
     1201 atcggcccgt atcacgtaca acaggcggct ggaaccgtcg tcgccgacga acacgatgcg
     1261 cgtgtgcgtc tccggaccct gcggaccggc gaccgactgc tcgctgcgct ccgtctcgaa
     1321 gtaaccggcg accgcgtaag ccgtggcgat ctcgtgaacg accccgggcg ccaagccgac
     1381 cgcgacgcca cccagcacgt aggcctccca gtccgtccac ttaggcgagt ccggcaggcg
     1441 tgcgaccagc gcaccgaccc cgtcgccgac cgcgcgcatg ctgccgcgcg gaaggctgac
     1501 gccagagtcg aactgagccg agatggcagc ggtcatcaac acgtccgcga ggagttgggc
     1561 gtgggcgctc gcaccgagga gggccgcgcc gctcagcacg caggcggtca acgcgctcct
     1621 ggcgaggcgc ttggcgcggt ccccgacgct cgagcggcgg ccgccccaac cgggtacgcg
     1681 ccgcctcccg gcgttcggga agagaccagc ggc
//
LOCUS       DAOQYE010000516         1711 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_85330_length_1711_cov_2.594203, whole genome shotgun sequence.
ACCESSION   DAOQYE010000516 DAOQYE010000000
VERSION     DAOQYE010000516.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1711
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85330_length_1711_cov_2.594203"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1670)
                     /locus_tag="PKN52_08465"
     CDS             complement(<1..1670)
                     /locus_tag="PKN52_08465"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNR00023.1"
                     /translation="MSNFEVEAVSADQVDATRLRLAAAVGDDYTVTLPATTGNFTFGI
                     VQTLQSGLSVLAATLLALGAFLAYNSLMATVVERSDEYALLRTICMTRADIRRLAVYE
                     ALVLAVLGVVAGILLGIGLSYLMTYVNAATLGYEFRTLVIPIKNVAAASVLGMVAALV
                     AGVVPALNAGKASPIASLQSAPGSVPRGSLLLGALAILLGVAAALLPWPGTTAIYATT
                     AALGLFFVGVSLTAPAMLPSLSRLARGPLTRLLGAPGRLGADAAQRNAGRNGVAIGTV
                     VVGTGLVIGVGSMVASTNRAISDWIDTTVVGDLFVTSPVTFPDDFAERAGAIEGVDVV
                     SGVKITAVRFKEDGGDSRGRSIALVLVDPERFNPRGGFGRFQYLPGQGDDEAGYQALL
                     TGDQLLAANTLRDRYGVESGASIELRTSDGFEAFRVAGVVVDFTGGGEAVIGSIAAIE
                     RFGGGNPNLYIFTVLPGQTQAAVRERLVAAFPELALDVTLNADYRANILAVTSQAFAT
                     TRILLAIAVLVAALGVANTLGMNLVNKGHEIAVLRTLGLTRGGVRALV"
BASE COUNT          274 a          580 c          622 g          235 t
ORIGIN      
        1 atcacgagcg cccgcacgcc gccgcgggtc aggccgagcg tgcgcagcac cgctatctcg
       61 tgacccttgt tgacgaggtt catgcccagc gtgttggcta caccgagggc cgccacgagc
      121 accgcgatgg cgaggaggat gcgggtggtc gcgaaggcct ggctggtcac ggcgaggatg
      181 ttcgcgcggt aatcggcgtt gagggtgacg tcgagcgcga gttccgggaa ggcggcgacg
      241 aggcgctcgc gcaccgccgc ctgcgtctgg cccggaagga cggtgaagat gtagaggttg
      301 gggttgccgc cgccgaaacg ctcgatggcg gcgatgctgc cgatgacggc ctccccgccg
      361 ccggtgaagt cgacgacgac cccggcgacc cggaacgcct cgaagccgtc cgaggtcctt
      421 agctcgatcg aggcgccgct ctcgacaccg taacggtcgc gcagggtgtt ggcggcgagc
      481 agctggtcgc cggtgagcag agcctggtag cccgcctcgt cgtcgccctg accgggcagg
      541 tactggaagc gtccgaagcc gcccctgggg ttgaagcgtt ccgggtcgac cagcacgagc
      601 gcgatcgagc gaccccggct gtcaccgccg tcctccttga agcgtacggc cgtgatcttc
      661 acgccgctca cgacgtcgac cccttcgatg gcgccggcgc gctccgcgaa gtcgtcgggg
      721 aacgtcacgg ggctggtcac gaacaggtcg cccaccacgg tcgtgtcgat ccaatccgag
      781 atggcgcggt tggtgctcgc caccatactg ccgacgccga tcactagccc cgtgccgacc
      841 acgaccgtgc cgatggcgac gccgttgcga ccggcgttgc gttgcgcggc gtctgcgccg
      901 agccgccccg gcgcgccgag aaggcgggtg agcggtcccc tcgccaggcg gctcagcgaa
      961 ggcagcatgg cgggggcggt cagcgagacg ccgacgaaga acagaccgag cgccgccgtg
     1021 gtggcgtaga tggccgtcgt gccgggccag ggcagcagcg ccgccgccac gccgagcaag
     1081 atggcgagcg caccgagcaa cagtgagccg cgcggcacgg agccgggcgc ggactggagc
     1141 gacgcgatcg gcgaggcctt gccggcgttc agggccggga ccacgcccgc cacgagcgcg
     1201 gccaccatgc cgaggacgct ggccgccgcc acgttcttga tgggtatcac gagcgtcctg
     1261 aactcgtagc cgagcgtggc ggcgttgacg tacgtcatga ggtaggagag gccgatcccc
     1321 aacaggatcc ctgccacgac gcccagtacc gccagcacga gcgcctcgta cacggcgagc
     1381 cgcctgatgt cggccctcgt catgcagatg gtgcgcagga gggcgtactc gtcactccgc
     1441 tcgaccaccg tcgccatcag gctgttgtac gcgaggaacg ctccgagcgc gaggagggtg
     1501 gcggcgagga cgctcaagcc cgactgcagg gtctgcacga tgccgaaggt gaagttgccg
     1561 gtggtggccg gcaacgtgac ggtgtagtcg tcgccgacgg cggcggcgag cctgagcctg
     1621 gtcgcgtcta cctggtcggc gctcaccgcc tcgacctcga agttagacac gcgcccttcc
     1681 aaccgcagca cccgctgaag gtccgccagg t
//
LOCUS       DAOQYE010000517         1710 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_85426_length_1710_cov_2.204230, whole genome shotgun sequence.
ACCESSION   DAOQYE010000517 DAOQYE010000000
VERSION     DAOQYE010000517.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85426_length_1710_cov_2.204230"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            136..861
                     /gene="surE"
                     /locus_tag="PKN52_08470"
     CDS             136..861
                     /gene="surE"
                     /locus_tag="PKN52_08470"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0008254 - 3'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HNR00024.1"
                     /translation="MSNDDGVFSPGIAALARVAAEFGQVRVVAPDVEQSAMSHAITVR
                     RPLHYTRTVIDGIEAFRVDGTPADCVALGAHHWDAVDLVLSGINLGYNIGHNIWHSGT
                     VAAAKQAAFLRIPAMAFSSAHLEAGTDYAKLAPFVGQAIKTFLGMPDMLLLNVNLPED
                     AKGVLWTRQSVRHYEGYVAEGVDPMGRTHYWFAEEPTEQVEEGTDRWAIENGYVSMTP
                     LRLDLTDEVLLARARAASGQAAG"
     gene            complement(869..>1710)
                     /gene="dinB"
                     /locus_tag="PKN52_08475"
     CDS             complement(869..>1710)
                     /gene="dinB"
                     /locus_tag="PKN52_08475"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HNR00025.1"
                     /translation="REASAKVRAVFRRYTELIEPLALDEAYLDVTDPLTGPPSATLIA
                     KAIKVDILRETGLTASAGVAAGKFLAKVASGMSKPDGLTVILPQQAEAFLATLAIERF
                     HGVGPRTAERMRALGILTGADLRARDPEELERHFGKSGRYFHAMACGVDDRPVEPNRE
                     RKSIGSETTLERDVARRADLEPLLVELCHDVARYMERHGLVARTVTVKLRFSDFRTVT
                     RSRTGMEPVPDASTLLAAAVRLAFEVDRPVLPVRLLGVSVSQLYPAGSLVVQERLRFP
                     PPP"
BASE COUNT          267 a          580 c          620 g          243 t
ORIGIN      
        1 cccattgtag atgacccact catgaaccgg atgccaaggg aacctaaagg ttcacggcag
       61 ccggcgcgac agcggcatat gatgctcgga aactttgtgc ccgcgcgcac atggagtggt
      121 gtgatgagga tcttggtgag caacgacgac ggggtgttca gccccggtat cgccgccctg
      181 gcgagggtag cggcagagtt cgggcaggtg cgggtcgtgg cgcccgacgt cgagcagtcc
      241 gccatgagcc acgccatcac cgtcaggcgc ccgcttcact acacgcgcac ggtcatagac
      301 ggcatcgagg ccttcagggt cgatggcacg cccgccgact gcgtggcgct aggcgcccac
      361 cactgggacg ccgtcgacct cgtgctgagc ggcatcaacc tcggctacaa catcggtcac
      421 aacatctggc actccggcac cgtggccgca gccaagcagg ccgcgttcct gcgcatcccg
      481 gccatggcct tcagctcggc ccacctggag gccggcaccg actacgcgaa gctcgccccg
      541 ttcgtcgggc aggccatcaa gaccttcctc ggcatgcccg acatgctgct gctgaacgtc
      601 aacctgccgg aagacgcgaa gggcgtgctg tggacgcgcc agtccgtgcg ccactacgag
      661 ggctacgtcg cggagggcgt cgacccgatg ggccggacgc actactggtt cgcggaggag
      721 ccgactgagc aggtcgagga gggcacggac cgctgggcga tcgagaacgg ctacgtctcc
      781 atgacaccgc tgcggctcga cctcaccgac gaggtgctgc tcgcgcgggc gcgggcggcg
      841 agcgggcagg ctgcgggcta ggcggcgatc atggcggcgg cgggaacctg agccgctcct
      901 gcacgacgag gctgccggct gggtagagct ggctgacgct caccccgagc agccgcacgg
      961 gcagcacggg ccggtcgacc tcgaacgcca gcctgaccgc cgccgccagc agcgtggaag
     1021 cgtcgggcac gggctccatg cccgtgcgac tgcgcgtaac ggtcctgaag tcgctgaacc
     1081 gcaacttgac ggtcacggtg cgggccacga ggccgtggcg ctccatgtac cgcgccacgt
     1141 cgtggcacag ctcgaccagc aggggctcga ggtcggcgcg gcgcgcgacg tcgcgctcga
     1201 gcgtcgtctc ggagccgatg gacttgcgct cccggttggg ctcgaccggc cggtcgtcga
     1261 cgccgcacgc catggcgtgg aagtatcgcc cggacttgcc gaagtggcgc tcgagctcct
     1321 cggggtcgcg cgcgcggagg tcggcgcccg tgaggatgcc gagcgcgcgc atccgctccg
     1381 ccgtgcgcgg gccgacgccg tggaatctct cgatggcgag cgtcgcgagg aacgcctcgg
     1441 cctgctgggg gaggatgacg gtcaggccgt cgggcttcga catgcccgag gccaccttgg
     1501 cgaggaactt gccggccgcc acgccggcgg aggccgtgag gcccgtctcg cgcaggatgt
     1561 ccaccttgat cgccttggcg atgagggtgg cggacggcgg gccggtcaac gggtcggtca
     1621 cgtcgaggta ggcctcgtcg agcgccagcg gctcgatcag ctccgtgtag cgcctgaaca
     1681 cggcgcgcac cttggcggac gcctcgcggt
//
LOCUS       DAOQYE010000518         1709 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_85502_length_1709_cov_2.043531, whole genome shotgun sequence.
ACCESSION   DAOQYE010000518 DAOQYE010000000
VERSION     DAOQYE010000518.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85502_length_1709_cov_2.043531"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1064
                     /gene="hisD"
                     /locus_tag="PKN52_08480"
     CDS             <1..1064
                     /gene="hisD"
                     /locus_tag="PKN52_08480"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HNR00026.1"
                     /translation="HEAQEGARAAQEDAAERVAAYYRLQAEGGFVHSAAGAVLGQLVV
                     PIGSVGCYVPGGTAPLFSTVLMTAVPARVAGVERIVVATPPGRDGRVPAEIRFAAHLA
                     GASELLCVGGPHAIAALAYGTESLARVDKVVGPGNRYVVEAKRQLYGTVGIEALPGPT
                     ETLVVADAEADVGHVVADLLAQAEHAAAQPVLVTPSLALVEAVVVALPGALAALSHPA
                     AAEESFRERGVAVLVDDLAEAIEVANAFAPEHLCLLVSAPWTLVGSVRNAGGLFLGAS
                     SPEALGDYVVGPSHVMPTGATARFSSFVNLRDFQKVIPFTQLTPAVVAELGPAAALMA
                     RAEGLEAHALAVEARLPRR"
     gene            complement(1094..1708)
                     /locus_tag="PKN52_08485"
     CDS             complement(1094..1708)
                     /locus_tag="PKN52_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNR00027.1"
                     /translation="MITVPLGELQANAYLLVEGADVVVVDPGGEGEALAAALRERGLR
                     LQAVWLTHAHFDHVGGLAALLRAAPGVPVHMHRGDEPLLSMAAAAAARWGIEIEQPPT
                     DYVPVDHGQTLRSGAIGATALHTPGHAPGHLAFYFPSAGAVLSGEALFKGSIGRTDLP
                     YCDHEQLLAGIRRELLVLPPETRVYPGHGEPTTVGAEAATNPFL"
BASE COUNT          223 a          603 c          637 g          246 t
ORIGIN      
        1 ggcacgaggc gcaggaaggt gcgcgcgctg cgcaggaaga tgccgcggaa cgcgtcgcgg
       61 cctactaccg gttgcaggcg gagggcggct tcgtacactc ggccgcgggt gcggtgctcg
      121 gccagctcgt agtacccatc ggcagcgtcg ggtgctacgt gccgggcggc acggcgccgt
      181 tgttctccac cgtcctcatg accgccgtgc ccgcccgggt cgccggcgtg gagcgcatcg
      241 tggtcgcgac cccgcctggc cgcgacggtc gcgtgccggc cgagatcagg ttcgccgccc
      301 acctcgcagg cgcgagcgag ctcctatgcg tcggcgggcc gcacgccatc gcggccctcg
      361 cctacggcac cgagagcttg gcccgcgtcg acaaggtcgt cggccccggc aaccgttacg
      421 tggtcgaggc gaagcggcag ctctacggca ccgtcgggat cgaggcgctg cccggtccga
      481 ccgagaccct cgtggtggcc gacgcggagg cggacgttgg ccacgtcgtc gccgacctgc
      541 tggcgcaggc ggagcatgcc gccgcgcagc ccgtcctcgt cacgccgagc ctcgctctcg
      601 tcgaggcggt ggtggtggcg ctcccgggcg ccctggccgc cttgagccat cctgccgcgg
      661 ccgaggagtc gttccgcgag cggggcgtgg cggtcttggt agacgacctt gcggaggcca
      721 tcgaggtcgc caacgctttc gcccccgagc acctctgctt gctggtgagc gccccgtgga
      781 ccttggtggg ctcggtgcgc aacgcgggcg gcctcttcct tggggccagt agcccggagg
      841 cgcttggcga ctacgtcgta ggcccgagcc acgtgatgcc gaccggcgcc acggcgcgct
      901 tcagctcgtt cgtgaaccta cgcgacttcc agaaggtcat ccctttcacc caactgacgc
      961 ccgccgtcgt ggcggaacta ggccctgcgg cggcgctcat ggcgagggcg gaagggctgg
     1021 aggcgcacgc gttggccgtg gaggcccgct tgccgcggcg gtaggcgagc ccctacagga
     1081 ggcgaacgga cccttacagg aacggattgg tggcggcctc ggcgccgacg gtcgtcggct
     1141 cgccgtggcc cgggtacacg cgggtctcgg gtggtaacac gagcaactct ctgcggatgc
     1201 ccgcgagtag ttgctcgtgg tcacagtacg gcaggtcggt acggcctatc gagcccttga
     1261 acagcgcctc gccggagagc accgcgccgg cgctcgggaa gtagaacgcc aggtgtccgg
     1321 gcgcgtggcc gggggtgtga agggccgtgg cgccgatggc cccggacctg agcgtctgcc
     1381 cgtgatcgac gggcacgtag tcggtaggcg gttgctcgat ctcgatgccc caccgggccg
     1441 cggccgccgc ggccatgctc aacaacggtt catcgccccg gtgcatgtgg acaggcacgc
     1501 ccggcgcggc gcgcaggagt gccgccaggc cgcccacgtg gtcgaagtgc gcgtgggtca
     1561 gccacacggc ctggagccgc aggccccgct cgcgcaacgc ggcggccagc gcctcgccct
     1621 ccccgccagg gtccacgacg actacgtcgg cgccctcgac gaggaggtag gcgttcgcct
     1681 gcaactcccc gagcgggacg gtgatcagg
//
LOCUS       DAOQYE010000519         1707 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_85647_length_1707_cov_2.687651, whole genome shotgun sequence.
ACCESSION   DAOQYE010000519 DAOQYE010000000
VERSION     DAOQYE010000519.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85647_length_1707_cov_2.687651"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..1174
                     /locus_tag="PKN52_08490"
                     /pseudo
     CDS             1..1174
                     /locus_tag="PKN52_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016099277.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            1219..>1707
                     /locus_tag="PKN52_08495"
     CDS             1219..>1707
                     /locus_tag="PKN52_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00028.1"
                     /translation="MTRRAPMPQRLFAGIFFVLAAIVCAVAPMPPVFRSLGIVLSAYL
                     GFSAAGMPVAYLCALLAPPFGLIGGDPDWLVMLPIVLSGNLLAMLGLEFGWRYGALVL
                     SPVLLVAPALVSWQLSKKPLFEVSLPWHVSEAAWVALHLLVAALGVLVALFLDRRREA
                     RAA"
BASE COUNT          275 a          637 c          547 g          248 t
ORIGIN      
        1 cgtcgagcac gacgaggaga ccatgcgcgc ggccgactac atcgtcgacc tcggacccgg
       61 cgccggggtc cacggcggca aggtggtcgc cgccggcacg ccagagctgc tcgcgcagga
      121 caggcagtcg ctcacggcca agtacctgcg gggcgagctg aagatcccgg tacctgaccg
      181 acgccgcgaa gggaacggca agaagctcgt catccaaggc gcgcgggagc ataacctccg
      241 tgacatcgac gtcgagatcc ccctcggtac cctcacgtgc gtgaccggcg cctccggctc
      301 gggcaagtcg accctcgtcc acggcatcct ccacgccgcc ctcgccaagc agctctaccg
      361 cgccaaggcc cagccgggcg cgcacaagcg catcaagggc accgagcacg tcgacaaggt
      421 gatcgagatc gaccagtcgc ccatcggccg gacgccccgc tccaaccccg ccacctacac
      481 cggcatcttc accgacatcc gcgacctctt cacgcgcgcg cccgaggcgc gcaagcgcgg
      541 ctacaagcca gggcgtttct ccttcaacgt caagggtgga cgctgcgagg cctgcaaggg
      601 cgacggtacc gtcaaggtcg agatgtactt cctgccggac gtgtacgtgc cgtgcgaagt
      661 gtgcaagggt gcgcgctaca accgcgagac gctcgaggtg aagatccgcg gcaagtccat
      721 agcggaagtg ctcaacatga ccaccgacga ggggctcgag ttcttcgaga acatcccggc
      781 catagcccgt aagctacagc tgatgcaaga cgtcggcctt ggctacctca agatcggcca
      841 gccgtcgccc acgctctcgg gcggcgaggc gcagcgcgtc aagctcgcga gcgagctcgg
      901 gcggcgcggc acgggccaga cgctctacat cctcgacgag cccaccaccg gcctccactt
      961 cgacgacacg cggaagctcc tcgaggtcct ccaccgcctc gtggacgccg gcaacacgct
     1021 cgtcgtgatc gagcacaacc tcgacgtcat caagaccgcc gactggatca tcgacctcgg
     1081 gcccgacgga ggcgcgcgcg gcggcaccat cgtggcggcc ggcacgccgg aagaggtggc
     1141 ggccaacgag gccagctcga cgggccagtt cctcctgcac gtgccagagg tgcgcgcggc
     1201 cgtgaggcgg agcgcggcat gacccgcaga gcgcccatgc cgcaacgcct cttcgccggg
     1261 atcttcttcg tcctggccgc gatcgtctgc gccgtggcgc ccatgccccc cgtgttcagg
     1321 agcctaggga tagtgctctc ggcgtacctg ggcttctccg ccgccggcat gcccgtcgcc
     1381 tacctgtgcg ccctgctcgc tccgccgttc gggctcatcg gcggcgaccc ggactggctc
     1441 gtcatgctcc ccatcgtgct gagcggcaac ctgctcgcca tgctcgggct cgagttcggc
     1501 tggcgctacg gcgcgctcgt cctctccccc gtcctcctcg tcgcgccggc gctcgtgtcg
     1561 tggcagctct ccaagaagcc actcttcgag gtgtcgctgc cctggcacgt cagcgaagcc
     1621 gcctgggtgg cgctgcacct gctcgtggca gcgctcggcg tgctcgtcgc gctcttcctg
     1681 gataggcggc gggaggcccg cgccgcc
//
LOCUS       DAOQYE010000520         1706 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_85738_length_1706_cov_2.568746, whole genome shotgun sequence.
ACCESSION   DAOQYE010000520 DAOQYE010000000
VERSION     DAOQYE010000520.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85738_length_1706_cov_2.568746"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(22..1242)
                     /locus_tag="PKN52_08500"
     CDS             complement(22..1242)
                     /locus_tag="PKN52_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00029.1"
                     /translation="MAGLAAAQTPPEPDVVFGQLEQAAAAVPTAGFDVTAALAALGTP
                     GEVPLETVFAFVRDDIGFTPYRGALKGPVGVLIDRAGNSLDRTLLLKALLAANGDTAI
                     VAHAVLDADTAAKVAAAGRSGAPFGAPAESLTDNAARLADLYGLDVADVMARLEGVAA
                     SKAALDRSIEERTAFQSSALAATLAEAGVQPSAAATGAEAAADHWWVQVQRDGAWVDL
                     DPTLPTSVVGDVVAPAQLRFDLTDVRLLAAVDGSCRDLSCGDRLHRVHVAAVVETWDG
                     ESLTETEVAATDLLAAEGFGRSVAFAAIPSDFPEDLDLYTTERPADAYRQALLEVTEW
                     QPALFVDGQTIGTSKVNADGTVGGGSSAGGAAGNAGMLGGGIGGMFGGFGGAAAGAED
                     DGPGAIRVELRSGL"
BASE COUNT          221 a          593 c          635 g          257 t
ORIGIN      
        1 ggtcatcatc gagcgggtgg cctacaagcc actgcgcaac tccacccgga tcgcacccgg
       61 gccgtcgtcc tccgctccgg cggccgcgcc gccgaagcca ccgaacatgc cgccgatgcc
      121 cccgccgagc atgccggcgt tgccggcggc ccccccggcg ctgctgccgc cacccacggt
      181 accgtcggcg ttcaccttgc tcgtgccgat ggtctggccg tcgacgaaca acgccggctg
      241 ccactccgtc acctcgagta gggcttgccg gtacgcgtcc gccgggcgtt cggtcgtgta
      301 caggtccagg tcctcgggga agtcgctcgg gatggcggcg aacgccacgc tgcggccgaa
      361 cccttcggcg gcgaggaggt cggtggcggc gacctcggtt tcggtgaggg actcgccgtc
      421 ccacgtctcc acgaccgccg ccacgtgcac gcggtgcagc cggtccccgc acgagaggtc
      481 gcggcacgag ccgtcgacgg cggccagcag gcggacgtcc gtcaggtcga agcggagctg
      541 ggccggcgca acgacgtctc cgacgacgct cgtcggcaag gtggggtcga ggtcgaccca
      601 ggcgccgtcg cgttgcactt ggacccacca gtggtcggcg gctgcctcgg cgccggtggc
      661 ggcggccgag ggttgcacgc cggcctccgc cagggtggcg gcgagcgccg agctctggaa
      721 agcggtccgt tcctcgatgc tgcggtccaa ggccgccttg gacgcggcca cgccttcgag
      781 gcgcgccatg acgtccgcga cgtcaaggcc gtagaggtcg gccaggcgcg ccgcgttgtc
      841 ggttagggac tcggccggcg cgccgaacgg cgcgccgctg cgcccggcgg cggcgacttt
      901 cgcggccgtg tcggcgtcga ggaccgcatg cgccactatg gccgtgtcgc cgttggcggc
      961 caggagagct ttgaggagca gggtgcggtc gagggagttg ccggcgcggt ctatcagaac
     1021 gccgaccggg cccttgaggg cgccgcggta cggcgtgaac ccgatgtcgt cgcgcacgaa
     1081 cgcgaagacg gtttccagcg gaacctctcc cggagtgccg agcgcggcca gggcagctgt
     1141 gacgtcgaag cccgccgtcg gtaccgcggc ggcggcctgt tcgagctggc cgaagaccac
     1201 atcgggctcg ggcggggtct gcgcggcggc caatccggcc aacgccaggc ccgctatcag
     1261 ggtgacggcg agacgccgta gccgccggag cggtgagcgg gtcgtcgacg cggtcgtcga
     1321 cgcggttgtt aacgcggtcg tcgacgcggt cgttgaccgg gtcggtgccg ggcgttcgcc
     1381 ggtgacgtcg cagcaggact ctgcgagcgc ttcgtgctgt gcgagacggg tatctgttat
     1441 caagggcgcc ttctcctcca tcgtcgattc ggtaggggta gaccctcact ggggcagacc
     1501 tcaactgtag ccccggtcgg aacgttcggc gggggcattc gtctcacttc tcccgtgcat
     1561 gccgcacgcg ccgcgagcct gccgcgcgta tggctcactg ggcgttgccc gtggtcccgg
     1621 ggcgccgcct cggccgatga cgtgcgtggg tgcccatccc cggcgcgtag gtcttaatcc
     1681 gacaagggcg taggtctaga gccacg
//
LOCUS       DAOQYE010000521         1704 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_85882_length_1704_cov_3.226804, whole genome shotgun sequence.
ACCESSION   DAOQYE010000521 DAOQYE010000000
VERSION     DAOQYE010000521.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85882_length_1704_cov_3.226804"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            114..1250
                     /locus_tag="PKN52_08505"
     CDS             114..1250
                     /locus_tag="PKN52_08505"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNR00030.1"
                     /translation="MFRAVVIAVVIGIGSVGVMFWALGEDISTALHVPFWAYLCGLGL
                     ACLNYLAGALRLRWLARHAGADLTFMSALRAYALGLTSAAITPSSAGQAPAVAFSLVA
                     GGMPAATAWTVNVRVWSLDLVFLGWSFPVSVLVLGRSTRLLGSARPELVAVGAFVFSL
                     LVVAVLIFRLDWVTGLAARALRLPGLRRWRDKTTDFLNRVEAASAGLQRAPLRLQAAL
                     HSATFLVYVTTYFTFFVMVVSMRPGAPLLPTMASAQVPSVASSFFPTPGGVGLLELGT
                     ASLMRLSSRSSVAASAPIAEHEPDRLEDLVGPAGGAAIVAATDDSSKARAPVAAAILA
                     WRIFTFYLRMLVGPVLSGAAWRRGVGGGDGKRKDAKRPEAGPDR"
     gene            complement(1334..>1704)
                     /locus_tag="PKN52_08510"
     CDS             complement(1334..>1704)
                     /locus_tag="PKN52_08510"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNR00031.1"
                     /translation="ISAFFLVALSTMAGVQGIDKVLLQAGSNFGANRWQMLRHVILPG
                     ALPIVFNGLRLALGTALIVIVAVEFVRAKTGVGYLVYYHWQVLGTPKMYAALLVVMGL
                     GVGLTALLQLVERLVMPWRR"
BASE COUNT          235 a          588 c          592 g          289 t
ORIGIN      
        1 cgtccgagga gctggacgat cagtcgaccg tgctgatgcg cgcggacacg aacctctacg
       61 aggccaaacg gctcggcaag aaccgcgtcg gcgcgtaggg gcgctcgggc cgcgtgttca
      121 gagccgtcgt catagccgta gtcatcggca tcggaagcgt cggcgtcatg ttctgggcgc
      181 tcggcgagga catcagcacc gccttgcacg tgccgttctg ggcttaccta tgcggcctcg
      241 gtctcgcctg cctcaactac ctggctgggg cgctgcgctt gcgttggttg gcacgccacg
      301 ccggcgccga cctcaccttc atgtcggcgc tgcgggcgta tgcgctcggc ctgacgagcg
      361 ccgccatcac tcccagctcg gcgggccagg cgcccgccgt cgcgttctcc ctcgttgcgg
      421 gtggcatgcc ggccgccacg gcctggacgg tgaacgtgcg cgtctggtcc ctcgacctgg
      481 tgttcctcgg atggtccttc cccgtgagcg tcctcgtgct cgggcgctcg acgcggctgc
      541 tcggcagcgc gaggccggaa ctcgtggccg tcggcgcgtt cgtcttcagt ctgctagtcg
      601 tggccgtcct gatcttccgg cttgactggg tgaccggtct ggcggcgagg gcgctgcggc
      661 tgcccgggct gaggcggtgg cgagacaaga cgacggactt cctgaaccgg gtagaggccg
      721 cgagcgctgg cctgcagcgc gcgccgctac ggctccaggc ggcgctccac tccgcgacct
      781 tcctcgtata cgtcaccacc tacttcacgt tcttcgtgat ggtggtctcc atgcggccgg
      841 gagccccgct ccttccgacc atggccagtg cccaggtgcc atccgtggcg tcgtcgttct
      901 ttcccacgcc cggcggagtc ggcctcctag aactcggcac cgccagcctc atgcgcttga
      961 gttcccgcag ctcggtcgcg gcgtccgcac cgatcgccga gcacgagccg gaccggctcg
     1021 aggacctcgt tggccccgcc ggcggggcgg cgatcgtggc cgcgacggac gactcgtcga
     1081 aggctcgcgc gcccgtggcg gccgccatcc tcgcgtggcg tatcttcacc ttctacttgc
     1141 gcatgttggt agggcccgtg ctcagcggtg ccgcatggcg ccgaggggtg gggggtgggg
     1201 acgggaagcg caaggacgcg aagcgtcccg aggccggccc cgatcgctag ccgctcggac
     1261 ctcgaaggag ccatcctcac acggcctgtc gtagcacgcg tcggaccaag gtcagccgcg
     1321 cgtcggaccc ggttcagcgc ctccaaggca tgacgaggcg ctcgacgagc tgaagtagcg
     1381 ccgtgaggcc caccccgagt cccatgacga cgagcagcgc ggcgtacatc ttgggtgtac
     1441 cgagcacttg ccagtggtag tagacgaggt agccgacccc ggtcttggcg cgtacgaact
     1501 cgacggccac tatcacgatg agcgccgtgc cgagcgccag cctgaggccg ttgaagacga
     1561 tcggcagggc gccgggcagg atgacgtgcc tgagcatctg ccagcggttg gcgccgaagt
     1621 tgctgccggc ttgcaggagg accttgtcta tcccctgcac gcccgccatg gtcgagagcg
     1681 cgaccaggaa gaacgccgag atgg
//
LOCUS       DAOQYE010000522         1702 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_86075_length_1702_cov_2.471160, whole genome shotgun sequence.
ACCESSION   DAOQYE010000522 DAOQYE010000000
VERSION     DAOQYE010000522.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86075_length_1702_cov_2.471160"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..744)
                     /locus_tag="PKN52_08515"
     CDS             complement(<1..744)
                     /locus_tag="PKN52_08515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00032.1"
                     /translation="MGLLVWVAVAVIVVWLLVRGSQARRDAQEVRDLPQADLLIEALY
                     RDGVRLGYKHAWAHQAEAERQRVAAFVRRRERVVVSVLVGGCALVGAALMGTAGVLPG
                     QSEWAPGVRTWHWLGLAAACAGLGVWLVRAMAPRDYARYSAAPESVATHMAAASHELE
                     EVAKVHAERQAELARSYLTKLIDERFTAGLEQGRRDAARLFNGADVQTRVSSAANAAY
                     VQGRADGANEAQKLAAEAYRINLAHLFDPM"
     gene            870..1058
                     /locus_tag="PKN52_08520"
     CDS             870..1058
                     /locus_tag="PKN52_08520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00033.1"
                     /translation="MAAEQMLLADATDDGAIVLRPMGVYPIEIYNEDRVQEFLDEDTL
                     PRALEELAAEHLSRRHGK"
     gene            complement(1377..1452)
                     /locus_tag="PKN52_08525"
     tRNA            complement(1377..1452)
                     /locus_tag="PKN52_08525"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1417..1419),aa:Lys,seq:ctt)
BASE COUNT          288 a          580 c          571 g          263 t
ORIGIN      
        1 catcggatcg aacagatggg ccagattgat ccggtaggcc tcggcagcga gcttctgcgc
       61 ctcgttggct ccgtccgcgc gcccttgaac gtaggcggcg ttggcggcgg acgagacgcg
      121 cgtctggacg tccgccccgt tgaagagccg cgccgcatcg cggcgaccct gctcgaggcc
      181 ggcggtgaag cgctcgtcga tgagcttcgt caagtacgac ctggccagtt cagcctgtcg
      241 ctccgcatgg actttggcca cctcctccag ttcgtgggag gccgcggcca tgtgggtcgc
      301 tacggactcg ggagccgccg agtatcgcgc gtagtcgcga ggcgccatgg ccctgaccag
      361 ccagacgccg agccccgcgc acgcggccgc cagcccgagc cagtgccacg tcctgacgcc
      421 cggcgcccac tcggattggc ccggcagcac gccggcggtg cccatgagcg cggcgccgac
      481 caaggcgcaa ccgcccacga gcaccgatac gaccacgcgc tcgcgacggc gcacgaaggc
      541 cgccacgcgc tgccgctccg cttcggcctg gtgcgcccac gcgtgcttgt agccgagcct
      601 cacgccgtct cggtagaggg cctctatgag gaggtcagcc tggggcaggt cgcggacttc
      661 ctgcgcgtcg cggcgggcct ggctgccgcg cacgaggagc cacacgacga tcacggcgac
      721 cgccacccat acgaggaggc ccatcagccc gccgcccgcg cgcgaggcac catccggtcg
      781 tagatgacta ggtcggtcac cagcgcctca aagcgtaccg aacagggcag ccgaagcagc
      841 gcaactacca accagcgtgg tagcgaccgt tggcagccga gcagatgcta ctggcggacg
      901 ccaccgacga tggcgccatc gttctacggc cgatgggcgt gtatccgata gagatctaca
      961 acgaggaccg cgtccaggag ttcctcgacg aggacaccct tccacgagcg ctcgaggagt
     1021 tggcggcaga gcacctctcg aggcgccacg gcaagtgagg cgggtcgacc gtgcgcgaac
     1081 ggtcgccggt cagatcgggc tcgtgtccga ggcgggcgaa gcgctcgtcg ctctgtccgc
     1141 gaagcacctt ccgctctagg acgcgcccca tcctggccgc ggccctaagc gccaacgcca
     1201 cggtgctcgt tacgggtgac cggcggcatt tcggcgcatt gttcggcaag acgctgaacc
     1261 gcacggtgac cgtcctcccg cccagggaag cactggagcg tctgctccgc cagtagtcgg
     1321 ggttggggtt cctgcagacg tgctccttta acgtgaagca cgtcctagct tgcgtgtggt
     1381 gggtcgtgta ggactcgaac ctacaacccg ctgattaaga gtcagctgct ctgccagttg
     1441 agctaacgac ccacggtgac gcgcttggcg cgcgaacatc gtgaagtgta cgcaatgggg
     1501 cctggcatgg tcaaccgccg gtcggtgaac cggtggcgag ttcgtgacca tccgatctgg
     1561 accgctcgca tgcgttcagc ggcgctccgc gtcggccgag tcgtctgttg acaacggctg
     1621 ttgcaagttc gtatgtttgt acaacagcac gtgtacggca agcaggcgcc caggcagata
     1681 gcgagggcgg agcagcaccc ca
//
LOCUS       DAOQYE010000523         1700 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_86196_length_1700_cov_3.237082, whole genome shotgun sequence.
ACCESSION   DAOQYE010000523 DAOQYE010000000
VERSION     DAOQYE010000523.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86196_length_1700_cov_3.237082"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            388..870
                     /locus_tag="PKN52_08530"
     CDS             388..870
                     /locus_tag="PKN52_08530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNR00034.1"
                     /translation="MAKKPLDLEREVIEAFSHALEVTEVLVAAVPDEYWHAPPLQKGR
                     TIAGIVAHVYGLRRTFTKMAKAPVGDALPSKTVTKAEALAALGENRLVLVDLFTGAVL
                     RGEGRIPGMPRRTAMMMAYLMQHEAHHRGHITRQLSEYGHKLSGEVTMRIWGWRKLES
                     "
     gene            940..1374
                     /locus_tag="PKN52_08535"
     CDS             940..1374
                     /locus_tag="PKN52_08535"
                     /inference="COORDINATES: protein motif:HMM:NF018663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNR00035.1"
                     /translation="MARFDPYLNFSGDAEEAMKFYQSVFGGELRVSRFSEFPGAADGV
                     PEGERDLLMHASLSLPDGRVLMASDAPSHMGRVQFGTSTHVMITVDSADEARRLYASL
                     GEGAKQVTMPLGDTGFAELYTSFQDRFGILWMIYFAGYTAAN"
     gene            1382..>1700
                     /locus_tag="PKN52_08540"
     CDS             1382..>1700
                     /locus_tag="PKN52_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00036.1"
                     /translation="MITHHEVDYMPPLAEGASKNDRYIELRRYVLALTEGEPDWLANL
                     ANVASAVFEHVPGLNWAGFYLMKGGELVLGPFQGRPACVRIAVGRGVCGAAVTERRTQ
                     VVPD"
BASE COUNT          267 a          552 c          612 g          269 t
ORIGIN      
        1 gggggggggc gcgtgtcggt ctggtggcgg cccgcggccg tcgcgccacg ctccgcggcg
       61 ctcggtaggg ctggctggtt gcgggttcgg ttgcgtgggg gtcggtcggc tcgcgcttga
      121 ctcaggccgc cgcggccagt cggtgacggg acagcggctg tccgttgggg cgagcgctcg
      181 cctagaccgc gactacggcc gctagggcgg cagcgtcgag cggatacgcg tagctatagt
      241 catagccatc gtccacggca ccacctcccg agagcggtct ggtcatggcg gagagtgtaa
      301 caggtgacgg ccgcgtgtca accggggtcg gcgtcctggt cgctgcactt gacggacgtg
      361 ccgaggcgcg tctaccgtac cggcgatatg gcgaagaaac cgctcgacct cgagcgtgag
      421 gtcatcgagg ctttctccca cgccctcgag gtcaccgagg tgctcgtggc ggccgtgccg
      481 gacgagtact ggcacgcccc gcccctccag aaggggcgca ccatcgccgg catagtggcg
      541 cacgtctacg ggctcaggcg cacgttcacg aagatggcga aggcgcccgt cggcgacgct
      601 ctgccgtcca agacggtcac gaaggcggaa gcgctcgccg ccctcggtga gaaccggctg
      661 gtcctcgtcg acctgttcac gggagccgtc ctgcgtggcg aggggcgcat acccggcatg
      721 cctaggcgca cggccatgat gatggcctac ctcatgcagc acgaggcgca tcaccgcggg
      781 cacatcacgc gccagctctc ggagtacggc cacaagctct cgggcgaggt gacgatgagg
      841 atctggggct ggcgcaagct ggagagttga gcccacgtgt atcgtgtgac tccagcgggt
      901 ggtgtcgagt ggcaggacgc ccggaatcga ggaacgaaga tggcgcggtt cgatccttac
      961 ctgaacttct ccggcgacgc cgaagaggcg atgaagttct accagtccgt gttcggcggc
     1021 gagttgcggg tctcacggtt cagcgagttc cccggtgccg ccgacggcgt tcccgagggt
     1081 gagcgcgacc tgctcatgca cgcctcgctc agcctgccgg acggcagggt cctgatggcg
     1141 agcgacgccc cgtctcacat gggcagggtc cagttcggca cctcgaccca cgtcatgatc
     1201 accgtcgaca gcgcggacga ggcgcggcgg ctctacgcga gcctcggcga gggggccaag
     1261 caggtcacga tgccgctcgg cgacaccggg ttcgccgagc tgtacacctc gttccaggac
     1321 cgcttcggca tcctgtggat gatctacttc gccggctaca ccgccgccaa ctgatgagca
     1381 gatgatcacg catcacgagg tcgactacat gccgcccctg gcggaaggcg ccagcaagaa
     1441 cgaccgctac atcgagttgc gccgctacgt gctcgcactt acggagggag agcccgactg
     1501 gctcgccaac ctggccaacg tggcgagcgc ggtcttcgag cacgtgcccg ggttgaactg
     1561 ggccggcttc tacctcatga agggtggcga actcgtgctc ggacccttcc agggcagacc
     1621 ggcgtgcgtg cggatcgcgg tggggcgcgg cgtgtgcggc gcggccgtca cggagcgccg
     1681 cacccaggtc gtgccggacg
//
LOCUS       DAOQYE010000524         1698 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_86393_length_1698_cov_1.878880, whole genome shotgun sequence.
ACCESSION   DAOQYE010000524 DAOQYE010000000
VERSION     DAOQYE010000524.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86393_length_1698_cov_1.878880"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            325..615
                     /locus_tag="PKN52_08545"
     CDS             325..615
                     /locus_tag="PKN52_08545"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HNR00037.1"
                     /translation="MRAIQEYLRFGALDEPIVYDAVRMRLIEVGEAVKSLDPELLADE
                     PGVPWRSIAGMRDRLAHSYFDTLVQVLTVTVTDDLGELEAAVARIAVRAEDG"
     gene            complement(779..1198)
                     /locus_tag="PKN52_08550"
     CDS             complement(779..1198)
                     /locus_tag="PKN52_08550"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNR00038.1"
                     /translation="MYLLDTNVVSELRKAGTGRAAPAVLRWASSADTADLHISVITVM
                     EIEIGVLRLERRDTAQGAAVRRWLEGNVLPAFAGRILPIDLTVVRHCARVLVPDPRPE
                     RDALIAATARAHDLTVVTRNTAGFQLAGIRALDPWKA"
     gene            complement(1199..1447)
                     /locus_tag="PKN52_08555"
     CDS             complement(1199..1447)
                     /locus_tag="PKN52_08555"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HNR00039.1"
                     /translation="MSTTVSSREFNQNISAAKRAADAGPVIITDRGQPAHVLMSFEHY
                     RHLTGARGDIVQLLAMPEDIDFEPARLESVGLAIPDLG"
BASE COUNT          279 a          502 c          582 g          335 t
ORIGIN      
        1 gttgggcctg cttcgaagcc ggcgtgacga gttgctctca gtcgggggtc cgatgggggt
       61 gcgaactatt cacgtgttcg gaagcgtggc acgtggcgag gatcgaccgg atagcgacgt
      121 ggaccttctc gtagagttgt ccagcccact cggtttcctc ggcttggctc ggatcgagga
      181 ggcgttcagt ggaatactcg ggcgtcgcgt cgagattgtt cccacctacg ctctcgtctc
      241 tgaggcaagt aagagccttt ggggcggcgc catccagttg tgagtcgctc ggatagggag
      301 cgactccagg acatactggt cgccgtgagg gcgattcagg agtacctgcg cttcggcgct
      361 ttggacgagc ccatagtcta cgacgcggtt cgcatgcggc tgatcgaggt tggcgaggcg
      421 gtgaagtccc tagaccccga gctgctggcg gatgagcctg gcgtgccttg gcgaagcata
      481 gctggcatgc gagaccggct ggctcacagc tatttcgaca cgttggtcca ggtcctcact
      541 gtgacggtta ccgacgatct cggcgagttg gaagcggccg tcgcgaggat agcggtgcgt
      601 gcggaggatg gttaggagcc gcccagtgcg ggacagcctt gttgtcgact ggtcaactca
      661 ccaaatgccc tgctcgattg ccctgtgagc ctattcgttt tcgacgatgg ggacgctgcc
      721 cgcgctcaga gaacccggtc tgcgcccgga ggaccctgcc cgcgctcggg cgcaacgctt
      781 acgccttcca tgggtccagt gctcggatac cggccagctg gaaacctgct gtgttgcgag
      841 tgaccacggt caagtcgtgg gctcgtgccg tggcggcgat gagggcgtcg cgctcgggcc
      901 tcggatccgg cacgagcaca cgtgcgcaat gtcggacgac ggtcaggtcg atggggagga
      961 tgcgtccggc gaaagcaggg aggacgttcc cctccaacca gcgtctgacg gccgctcctt
     1021 gggccgtgtc gcggcgttcg aggcggagga cgccgatctc gatctccatg accgtgatga
     1081 ccgagatatg caggtcggct gtgtcagctg aactcgccca gcgcaggacg gcgggcgctg
     1141 cgcggccggt gccggccttg cgcaactccg acacgacgtt cgtatccagg agatacacct
     1201 aaccgaggtc cgggatcgcc aagcctaccg actcgaggcg ggcaggctcg aagtcgatgt
     1261 cctccggcat ggcgagtaac tgcacgatgt caccgcgcgc accagtcaag tgccggtaat
     1321 gctcgaagct catcagcacg tgagcgggtt gaccccggtc cgtgatgatc acgggccccg
     1381 cgtctgccgc gcgtttggcg gcgctgatgt tctggttgaa ctctctgctc gatacggtgg
     1441 tcgacatcgt tcccctccga tgtagtaacg atactacatc ccgtcgccag ccagggctgt
     1501 ctggatgccc actcggtctc ctggggctgt tccgaatcga ggaggcgttc agcgacatca
     1561 tcggccgccg cgtcgaggtc gttccaacgt acgccctcgt ttcggagtcg agtcgacttc
     1621 tatgggacga ggctgttcct gtctgaggcg ctaggagggg gcgaggcatg tgagtaagct
     1681 ggccgccacc aagccggc
//
LOCUS       DAOQYE010000525         1697 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_86471_length_1697_cov_2.048112, whole genome shotgun sequence.
ACCESSION   DAOQYE010000525 DAOQYE010000000
VERSION     DAOQYE010000525.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86471_length_1697_cov_2.048112"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="PKN52_08560"
     CDS             <1..351
                     /locus_tag="PKN52_08560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00040.1"
                     /translation="DHRLAFALPVPAHDAWLVALAQGPAPAGGWWTTLQTHLAALSNP
                     ILLDGVLGVPLDEQDASAAGRVLEGAMELLLEERQRSRDRRDFARADTLRDRLAEMGV
                     SIEDTSEGSRWRLE"
     gene            430..1680
                     /gene="dprA"
                     /locus_tag="PKN52_08565"
     CDS             430..1680
                     /gene="dprA"
                     /locus_tag="PKN52_08565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HNR00041.1"
                     /translation="MAGVADELAAQYLAALAMPGVGPVRLGRLVRAYGGLNAVMAGGS
                     PTAETDDGAALARFAEALRDALPSYLPRARREVAEARAGGLSVTCWGEPDYPPALLED
                     ESGLSPVLFVAGELPPQVTLGSRELESCALVGTRRASQHALSFARDLARSAALHGLVV
                     VSGLALGVDSAAHAGALEAPPGAGTTVAVLGGGHDRLHPAGNRGLAERIVRHGGAVVS
                     EWPPAVNPQRHHFLRRNRLIAGLARVVAVVEAGVRSGALNTATHAAELDRPVLAVPGR
                     PHDERSRGALELLREGAAPLIDESDLLHHFGISGKTKGPAAGSSGAGGGIPDGEYVRE
                     PSPGSPGLGGATSRSASGSVAGSATASTTAKLRAAFRGAEATLDQLGRATGLPAGDLL
                     AALALMELDGTVASAPGRRYRWRG"
BASE COUNT          189 a          630 c          643 g          235 t
ORIGIN      
        1 gaccaccgtc tcgcgttcgc gctccccgtg cccgcgcacg acgcgtggct cgtcgcgctg
       61 gcccagggtc cggcgccggc cggcgggtgg tggacgacgc tgcagacgca cctcgccgcg
      121 ctgtcgaatc cgatcctgct cgacggcgtg ctcggtgttc cgctcgacga acaggacgcg
      181 tccgcggccg gtcgcgtgct ggaaggggcc atggagctgc tcctcgagga gcggcagcgc
      241 tcgcgcgacc ggcgtgactt cgcgcgcgcc gacacgctgc gcgaccggct cgccgagatg
      301 ggtgtgagca tcgaggacac gtccgagggg agccgctggc ggttggagtg aggccggtcg
      361 cccagatcgc ctaggctcca ggcctgagcc gcggcgcatg ggccgcgctc cgcgccgcat
      421 gctggaccca tggcgggggt agcggacgaa ctggcggcgc agtacctcgc ggcccttgcc
      481 atgccgggcg taggcccggt acgccttggg cgtctcgtca gggcttacgg gggtctgaac
      541 gctgtcatgg cgggcggctc acccaccgcc gagaccgacg acggcgcggc gctcgcgcgc
      601 ttcgccgagg ccttgcgaga cgcgctgcct tcttaccttc cccgcgcgcg gcgcgaggtc
      661 gccgaggctc gggccggtgg gctctcggtc acgtgctggg gcgagccgga ctacccgccc
      721 gcgctcctcg aggacgagag cggcttgtcg cccgtgctgt tcgtggcggg tgagcttccg
      781 ccgcaggtca ccctcggttc ccgggaactg gagagctgcg ccttggtagg cacccggcgc
      841 gcctcgcagc atgcgttgtc gttcgcgcgc gacctggcgc gttcggcggc gctccacggt
      901 ctcgtcgtgg tctcgggcct cgcgctgggt gtcgactcgg cggcccacgc gggggcgctc
      961 gaggcgcccc cgggcgcggg tacgaccgtg gcggtcctcg ggggcggcca cgaccggctc
     1021 cacccggccg gtaaccgagg cctggccgag cgcatcgtcc gccatggggg cgcggtggta
     1081 tccgaatggc cgcccgccgt caacccgcag cgccaccact tcctgcggcg caaccgcctg
     1141 atcgccggcc tcgctcgcgt agtcgccgtc gtggaggcgg gcgtgcgctc cggcgccctc
     1201 aacacggcca cgcatgccgc cgagctcgac cggcctgttc tggcggtgcc gggtcgtccc
     1261 catgacgaac gcagccgcgg agccctggag ctgctccggg agggggcggc gcccctgata
     1321 gacgagagcg acctcctgca tcacttcggc atctcgggaa agaccaaggg gccggccgcc
     1381 ggctcctcgg gcgcgggcgg agggatcccg gacggcgagt acgttcgcga gccgagcccc
     1441 ggctcaccgg gcctgggcgg agcgacctcc cgctccgcgt ccggttcggt ggccggttcc
     1501 gcgaccgcct cgacgacggc gaagctgcgt gccgcgttcc gcggcgccga ggcgaccctg
     1561 gaccagctcg gccgcgccac cggcctcccc gccggcgacc tgctcgcggc actggcgctc
     1621 atggagctcg acggcacagt ggctagcgcg ccgggccgca ggtaccgctg gcgtggctga
     1681 ggccgggcga ccagacc
//
LOCUS       DAOQYE010000526         1692 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_86844_length_1692_cov_2.824068, whole genome shotgun sequence.
ACCESSION   DAOQYE010000526 DAOQYE010000000
VERSION     DAOQYE010000526.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86844_length_1692_cov_2.824068"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..962)
                     /locus_tag="PKN52_08570"
     CDS             complement(<1..962)
                     /locus_tag="PKN52_08570"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HNR00042.1"
                     /translation="MARELRLPELAESVVEGEIIRWLVAEGDSVALDQPVAEVLTDKA
                     TVELPSPFAGTLLKHVAAEGDIVQVNAVIALIEESDASTAGKAPPTAPASGAGSAGKQ
                     VGSAHAPGGAGPSGGGASGAGASGGGASAAETVDDGDKLSLFKPSAGGDTEALPRMQR
                     PADRSSGTRLGREPARGAFGRVLAVPAARKLARELGVSIEEVLGSGPHGRVRVDDVRG
                     HVPPAAGQGQTGPQGSPLPYKTPAGYEDREERAPMRGLRRLTANQMVTSHLHTVRALH
                     VDEADVTDLVALREKLKPSFAAKGVRLSYLPFVMKAVAVALAEFP"
     gene            complement(1049..>1692)
                     /locus_tag="PKN52_08575"
     CDS             complement(1049..>1692)
                     /locus_tag="PKN52_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HNR00043.1"
                     /translation="LVVRMPAGGGVKGGHHHSQSPEAHFVHTAGLKVVFPSTPSDARG
                     LLKSAIRDDDPVVFMEPKRLYRAVKEELPDDLDALVPIGKAAVRRVGDDVVLVSYGGS
                     MAETARAAEALAEQGVSPHVIDLRSLAPWDEEAVLEAVARVGRVVLVSEAPRTASVMS
                     EVAATITEELLDQLLAPPLRVSGFDTPYPYAQDREYLPGPARIMRAVQRVLDY"
BASE COUNT          215 a          611 c          624 g          242 t
ORIGIN      
        1 gggaactcgg cgagggcgac ggccacggcc ttcatcacga acggcaggta cgagagccgc
       61 acccccttgg cggcgaagct cggcttgagc ttctcgcgca gtgccacgag gtccgtgacg
      121 tccgcctcgt ctacgtgcag cgcgcgcacc gtgtgcaggt gcgacgtgac catctggttg
      181 gccgtcagcc ggcgcaggcc acgcatgggc gcgcgttcct cgcggtcctc gtagccggcg
      241 ggcgtcttgt agggcaacgg gctgccttgt ggcccggttt ggccttgacc ggcagcgggg
      301 ggcacgtgtc ccctcacgtc gtcgacgcgc acccgcccgt gcggcccgct gccgaggacc
      361 tcctcgatgc tcacgccaag ctcgcgcgcg agtttgcgcg ccgcggggac ggcgaggacg
      421 cggccgaacg cgccgcgggc aggctcccga ccaagccgcg tgccgctcga ccggtccgcc
      481 gggcgctgca tccgcgggag cgcctcggtg tcgccccccg cagacggctt gaagaggctg
      541 agcttgtcgc cgtcgtcgac tgtctcggct gctgacgcgc ccccgccgga cgcaccagcg
      601 ccggacgcgc ccccgccgga cggcccggcg ccgcccggcg cgtgggccga gccgacctgc
      661 ttgccagcgg agccggcgcc ggacgcggga gcggtcggcg gcgccttacc cgccgtggag
      721 gcgtccgact cctcgatgag cgcgatcacg gcgttgacct gcacgatgtc gccttcggcg
      781 gccacgtgct tgaggagcgt gccggcgaac gggctgggga gttcgacggt cgccttgtcg
      841 gtgaggactt cggcgacggg ctgatcgagg gccacgctgt cgccttcggc gacgagccaa
      901 cggatgatct ccccctcgac gacggactcg gccagttcgg gtaggcgtag ctctcgcgcc
      961 attctccaac tccttaggtt gcggccggcg ccacggcacg gcgcggcacc cgctcgaccg
     1021 cccggcggcc gggggcggcg gccttcactc agtagtcgag cacgcgctgc acggcccgca
     1081 tgatccgtgc cgggccaggc aggtactcgc ggtcttgcgc gtacgggtag ggggtgtcga
     1141 agccgctgac ccgcaacggg ggcgcaagca gctgatcgag caactcctcg gtgatggtgg
     1201 cggccacctc gctcatcacg ctcgcggttc tgggcgcctc gctcacgagc acgacgcggc
     1261 cgacgcgggc gacggcctcg aggacggcct cctcgtccca tggcgcgagc gagcgcaggt
     1321 cgatgacgtg aggcgagacg ccttgctcag cgagcgcctc cgcggcacgc gccgtctcgg
     1381 ccatggagcc gccgtagctg acgagcacga cgtcgtcgcc aacgcgcctg acagccgcct
     1441 tgcctatcgg cacgagcgcg tcgaggtcgt cgggcagctc ctccttgacg gcgcggtaga
     1501 ggcgcttcgg ctccatgaac acgacggggt cgtcgtcgcg gatggcgctc ttgagcagcc
     1561 cgcgcgcgtc ggacggcgtg ctcgggaaca cgaccttgag gccggcggta tgcacgaagt
     1621 gcgcctcggg gctctgggag tggtggtggc cccccttgac gcccccgccg gccggcatgc
     1681 gcaccacgag gg
//
LOCUS       DAOQYE010000527         1690 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_87031_length_1690_cov_2.477676, whole genome shotgun sequence.
ACCESSION   DAOQYE010000527 DAOQYE010000000
VERSION     DAOQYE010000527.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87031_length_1690_cov_2.477676"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="PKN52_08580"
     CDS             <1..324
                     /locus_tag="PKN52_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNR00044.1"
                     /translation="NVRALLAVPSGPQSVIVKRGANGASVFLRDGTVTDVPGFPVEVH
                     NVLGAGDAFASGFIYGRLKGWDWYRSARMGNACGAIVVTRHGCANFMAYEDEALAFVE
                     KHGGF"
     gene            complement(396..>1690)
                     /locus_tag="PKN52_08585"
     CDS             complement(396..>1690)
                     /locus_tag="PKN52_08585"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HNR00045.1"
                     /translation="VSAAEATPGYIAGPLQAGRWQVVLGLALVDEGGCDYRVAVEVTA
                     ASSGAEHAGPPPDRPTTKGSYSIGLGAGRQVGHANAYPSAHPVSSAASRSWYRGDLQS
                     HTYYSDARGSLPDLVAEARSRALDFLAITDHNTSSHHLELGTWSNSAGLLLVPGEEVT
                     TYRGHANVWGVGGWTDFRIERPGDLASLVRQVNERGGLFSVNHPKVTPGCLGCDWEYE
                     VPDGAASLEAWQGPWLMRNWDSLARYDTLLRAGRKLTLVGGSDRHHPGYPNHDQPFLQ
                     VGSPTTWLELAELSVPAVLDAIRVGRAFVSETPEGPFLELDVGGVGMGGTVATGRAVT
                     AAARVAGAAGDLLTWIGSDGVLREVVIEGDDFTDAWEWLPGGAYLRVEVVARASLPGF
                     LDELARFEAAGRLPPYLKHEEVVANPWRRALSNPVYVA"
BASE COUNT          230 a          610 c          616 g          234 t
ORIGIN      
        1 aacgtgcgcg cgctcctcgc cgtgccgtcg ggcccgcagt ccgtcatcgt gaagcggggg
       61 gccaacggcg cgagcgtgtt cctgcgcgac ggcaccgtga ccgacgtgcc ggggttcccc
      121 gtcgaggtgc acaacgtcct cggcgccggc gacgcgttcg ccagcggctt catatacggt
      181 cgcctcaagg gttgggactg gtaccggagc gcgcgcatgg gtaacgcgtg cggcgccatc
      241 gtcgtcacgc gccacgggtg cgcgaacttc atggcctacg aggacgaggc gctcgcgttc
      301 gtcgagaaac acggggggtt ctaggcccgg cggggtcggt cgagcctgcc cagtgcgatc
      361 ggggacgcgt gggcgccgag accgtcgcca tgcccctacg ctacgtagac cgggttggag
      421 agcgcccgcc gccacgggtt cgcgacgacc tcctcgtgct tcaggtacgg cggcagccgg
      481 cccgccgcct cgaagcgcgc caactcgtcg aggaagccgg gcagggaggc ccgggccacc
      541 acctccacgc ggaggtacgc gccgcctggc aaccactccc aggcgtccgt gaagtcgtcg
      601 ccctcgatga cgacctccct gagcacgcca tcgctgccga tccaggtgag caggtcgccg
      661 gccgcgcccg ccacgcgcgc ggcggccgtg accgcccggc ccgtggcgac tgtgccgccc
      721 atcccgacgc cgcccacgtc gagctcgagg aacggccctt ccggggtctc gcttacgaaa
      781 gcgcggccga cgcggatcgc gtccagaacc gccgggaccg acagctcggc gagctcgagc
      841 cacgtcgtgg gcgagccgac ctgcaggaag ggttggtcgt ggttcgggta gccggggtgg
      901 tgcctgtcgc ttccgccgac cagcgtgagc ttccgaccgg cgcgcaggag cgtgtcgtag
      961 cgggcgaggg agtcccagtt gcgcatcagc cacggcccct gccacgcctc gaggctggcg
     1021 gcgccgtcgg ggacctcgta ctcccagtcg caccctaggc agccgggcgt caccttcggg
     1081 tggttgacgg agaagaggcc gccgcgctcg ttcacctggc gcacgaggct cgccagatcg
     1141 ccgggccgtt cgatccggaa gtcggtccac ccgcctacgc cccacacgtt cgcgtggcca
     1201 cggtaggtag tcacctcctc gccaggcacg aggagcaggc cggccgagtt cgaccacgtc
     1261 ccgagctcga ggtggtggct ggaggtgttg tgatcggtga tggcgaggaa gtccaacgct
     1321 cggctccgcg cctccgccac gaggtcgggc agcgagccgc gggcgtcgct gtagtacgtg
     1381 tggctctgga ggtcgccgcg gtaccagcta cggctcgcgg cggacgagac agggtgtgcg
     1441 gaagggtagg cgttcgcgtg ccccacctgc cgaccggcgc cgaggccgat cgaatagctg
     1501 cccttcgtgg tcggcctgtc cggcggtggc ccggcgtgct cggcccccga cgacgcggcg
     1561 gtcacctcga ccgcgacccg gtagtcgcag ccgccctcgt ccacgagcgc gagcccgagc
     1621 acgacctgcc accgcccggc ctgcagcggc cccgcgatgt agccgggggt ggcttccgcg
     1681 gcgctcacga
//
LOCUS       DAOQYE010000528         1689 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_87093_length_1689_cov_3.219706, whole genome shotgun sequence.
ACCESSION   DAOQYE010000528 DAOQYE010000000
VERSION     DAOQYE010000528.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87093_length_1689_cov_3.219706"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            61..1482
                     /locus_tag="PKN52_08590"
     CDS             61..1482
                     /locus_tag="PKN52_08590"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNR00046.1"
                     /translation="MRNLSIRPPAFRGRAWTLVVIIALPFALLACGPSKREQELTAEN
                     ATLTSRVASLQSDLEQAAAAKTTLEASLATARDALATAEGERDAATASVAELQDTVTG
                     LEARLTEAQTRFDAASARLDAVNAQLASATNELTQAQARVEEVEAQADEARAEADEGQ
                     ARSQEAQDRVDEIAAVRVESATAAARAEAEGLRSEVDQANAELDDLKVVYDAAERELG
                     QLRVARDDLERQVVALAGERDAIEVELQTAQAAATAAKAAVAEAVEAANGAEARVQEA
                     SMSLAIVMQQRDEVVAENARLASERADLQTQRDEALSQRVQMASELADLAAQRDAAVA
                     DLDRLSATVEASQAVRSELQQRLGATATDLDGARSRAADLTRQLEALTDQRSQLEQVS
                     EARQDELARVRSELEAAQQQVATLTGARGIYTVQPLDSLSSIATFFYRQGALWPRVLE
                     ANAHLIDDPNLIFPGMVLIVPQL"
BASE COUNT          290 a          573 c          598 g          228 t
ORIGIN      
        1 atggtcgcgc cggctctcat gagtctcgtc tgaacgggct cgcgccggat atattcagtc
       61 atgcgcaact tgagcatccg gccgccggcc ttccgggggc gagcgtggac cttagtggtg
      121 atcatcgccc tgccgtttgc gttgctcgcg tgcggaccaa gcaagcgcga gcaggagctg
      181 acggctgaga acgccactct caccagtcgc gtggccagcc ttcagtcgga cctcgagcag
      241 gccgctgccg ccaagacgac cctggaagcg agcctcgcca ccgcgcgcga cgctctcgcg
      301 accgcggaag gcgaacgcga cgccgcgacc gcttccgtcg ccgagctcca ggacacggtg
      361 acggggctcg aggctcgact gaccgaggcc cagacgcggt tcgacgcggc ttcagcccga
      421 ctcgacgccg tgaacgccca gctcgcctcc gcgaccaacg aactcactca ggcgcaagcc
      481 agggtcgagg aggtggaggc tcaggccgac gaggcgcgcg ccgaggccga cgagggccag
      541 gcccgctcgc aggaggctca ggaccgagtg gatgagatcg cggccgtccg ggtggagtcc
      601 gccaccgccg cggccagggc ggaggccgag ggcctgaggt ccgaggtcga ccaggccaac
      661 gccgagttgg acgacctgaa agtggtgtac gacgcggccg agcgtgaact ggggcagttg
      721 cgggtcgcta gggacgatct ggagcgccag gtcgttgcgc tcgcaggcga gcgagacgcc
      781 atcgaagtgg agttgcagac ggcacaggcg gccgcgaccg ctgccaaggc cgcggtggcg
      841 gaggcggtgg aggccgccaa cggggcggag gcacgggtgc aggaagccag catgagcctc
      901 gccatagtca tgcagcagcg cgacgaggtc gtcgcggaga acgcccgcct cgcttccgag
      961 cgcgccgacc tgcagaccca gcgcgacgag gcgctgagcc agcgcgtgca gatggcgtcc
     1021 gaactcgcag acctggccgc tcaacgcgat gcggcggtgg cggatctgga ccgcctcagc
     1081 gctacggtcg aggcctcgca ggcggtgcgc tccgagctgc agcagcgcct tggcgctacg
     1141 gccaccgacc tggacggggc tcgttcgcgt gccgccgacc tgacgaggca gctcgaggcc
     1201 ctgaccgacc agcggtccca gttggagcag gtaagcgagg ccaggcaaga tgaactcgcg
     1261 cgcgtcaggt ccgaactcga ggcggcgcaa cagcaggtgg ccaccctcac gggcgcgcgg
     1321 ggcatctaca cggtgcagcc cctcgactcc ctctcctcca tagccacgtt cttctaccgc
     1381 cagggcgcgt tgtggccgcg cgtgctggag gccaacgccc atctgatcga cgacccgaac
     1441 ctgatcttcc ctggcatggt gttgatagtc ccgcaactct agccagcgag tcttgcgact
     1501 ccggccagca agccgtaccg cggagttcgc tcgcggcgag cggggagcat tacgcttgcg
     1561 gcatggtcgc cgagacgatc tacaccatcg gccactccac tcgtccggtg cgggagttca
     1621 tcgacatgct ccatgcgcac ggggtgaagc agctggtcga cgtgcgcacg gtaccgaagt
     1681 cgcgccaca
//
LOCUS       DAOQYE010000529         1689 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_87115_length_1689_cov_2.506120, whole genome shotgun sequence.
ACCESSION   DAOQYE010000529 DAOQYE010000000
VERSION     DAOQYE010000529.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87115_length_1689_cov_2.506120"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="PKN52_08595"
     CDS             <1..478
                     /locus_tag="PKN52_08595"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNR00047.1"
                     /translation="VESGDGPYEIKVPDRCSCLINWHLVPGESADDVVGELRALAESI
                     SPAVDALFEVMPPAYPSYLTAPDHPFLKHFAKVYRREFGHDPAFAYGRGVSDANLLAA
                     SGIPAVMFGPSGANLHAADEWVDVRQIGEAARFYVALATSGQAWTSGTSGHRKDHP"
     gene            475..>1689
                     /locus_tag="PKN52_08600"
     CDS             475..>1689
                     /locus_tag="PKN52_08600"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HNR00048.1"
                     /translation="MTTSALPSILEAFDLDRAMAHVRWLSERTPNRISGGGQDRLAAE
                     YIRDTLASYGLAAGLQEFDTYTSTIGSGSLDVLSPEPWTPVVKPCLHIDPTPPGGVVA
                     ELVAVGPGGREDYVGLDVRGKIVLAEVSYAPATPEKARLAYEQGAIGIVLMNWGTSDQ
                     DNIPWRALKAVWGNPTRATWNEIPRLHALAITRRDGERLKAQCRQGKVEVRLRVTGQR
                     LWARVAQPIAWLHAPESSPEREQFVVVSGHLDAWEPGVTDNASGNGVMIEIARLLAER
                     RVELRRSVVFCFWNGHEIAEAAGSTHFVDTHWEELNRNGVAYTNIDSVGMRGADHLAV
                     NSSPELRDAHQAIAEELFGERLPSKRLERIGDQSFFGVGVPAISARHALPDAMVRAWN
                     GATLGWWNHTDKD"
BASE COUNT          280 a          596 c          568 g          245 t
ORIGIN      
        1 cgtggagagc ggcgacgggc catacgagat caaggtcccc gaccgctgct cctgcctgat
       61 caactggcat ctcgtccccg gcgagagtgc cgacgacgtg gtgggcgagc tgcgggcgct
      121 ggcggagagc atctcacccg cggtggacgc cctgttcgag gtcatgcccc cggcctaccc
      181 cagttacctc accgcgcccg accacccctt cctgaagcac ttcgccaagg tctaccgccg
      241 cgagttcggc cacgacccgg ccttcgccta cggccgcggg gtgagcgacg ctaacctctt
      301 ggccgccagc ggcatcccag cggtcatgtt cggtccgtcc ggggcgaacc tgcacgccgc
      361 cgacgagtgg gtggacgtga ggcagatcgg cgaggcggca cgtttctacg tggcgctggc
      421 gacctccggg caagcctgga cctcagggac atcagggcat agaaaggacc acccatgacg
      481 acttcggctc taccgtcgat cctcgaagct ttcgacctcg accgggccat ggcccatgtc
      541 cgctggctga gcgagcggac ccccaaccgc atctccggcg gggggcagga ccgcttggcc
      601 gccgagtaca tccgtgacac gctggcctcc tacggcctgg cggccgggct ccaggagttc
      661 gacacctaca ccagcaccat cggcagcggc tcgcttgacg tcctctcccc agagccttgg
      721 acgcccgtcg tcaaaccgtg cctgcacatc gacccgaccc cgccgggggg tgtggtggcg
      781 gagctcgtcg cggtaggccc cggcgggcgc gaggattacg ttggcctcga cgtacgcggc
      841 aagatcgtcc tggcggaggt cagctacgcc cccgcgacac ccgagaaagc ccgactggcc
      901 tacgagcagg gagccatcgg catcgtcctt atgaactggg gcacctcgga tcaggacaac
      961 atcccatggc gcgccctgaa ggccgtatgg ggcaacccca ccagggccac ctggaacgag
     1021 atcccgaggc tgcacgccct cgcgatcacc cgtcgcgacg gcgaaaggct caaggcgcag
     1081 tgccggcagg gcaaggtgga ggtaaggctg cgtgtgaccg gccaacgcct ctgggcgcgg
     1141 gtcgcgcagc cgatcgcctg gctgcacgcg cccgagtcca gcccggagcg cgagcagttc
     1201 gtggtcgtct cggggcacct cgacgcctgg gagccaggcg tgaccgacaa cgccagcggc
     1261 aacggcgtga tgatcgagat cgccaggctg ttggcggagc gacgtgtcga gttgcgccgc
     1321 tcggtggtct tctgcttctg gaacggtcat gagatcgccg aggccgccgg ctccacccac
     1381 ttcgtcgata cccactggga ggagctgaac cgtaacggcg tggcgtacac caacatcgac
     1441 tcggtcggca tgcgcggcgc cgaccacctg gccgtcaaca gcagccccga gctgcgcgac
     1501 gcgcatcagg cgatcgcgga ggagctcttc ggcgagcgac tgccgagcaa gcggttggag
     1561 cggattggcg accagtcctt cttcggtgtc ggcgtgccgg ccatctcggc ccgccacgcc
     1621 ctgccggacg ccatggtgcg ggcctggaac ggcgccaccc tcggctggtg gaaccacacc
     1681 gacaaggac
//
LOCUS       DAOQYE010000530         1689 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_87123_length_1689_cov_2.364749, whole genome shotgun sequence.
ACCESSION   DAOQYE010000530 DAOQYE010000000
VERSION     DAOQYE010000530.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87123_length_1689_cov_2.364749"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1147)
                     /gene="bshC"
                     /locus_tag="PKN52_08605"
     CDS             complement(<1..1147)
                     /gene="bshC"
                     /locus_tag="PKN52_08605"
                     /inference="COORDINATES: protein motif:HMM:NF021586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HNR00049.1"
                     /translation="MPPRATPPTDFAHAYSSGSLDDFFEVAPGDVDKALALPRPSERA
                     SLVAALKRHAIALGAPPPVLANLERLSHPEARAVVTGQQTGLLLGPTYSLSKAMTAVA
                     LARRLDTRERPVVPIFWLATQDHDAHEMDNTYMLDASETLRRLSVRLPDGVAVGRVPL
                     TREMVDGVSAGLAALTPRPRFLEPVWELLEGAASSVTTYSDWFAALFTRLLGPAGLVL
                     IDPLRSDVAEHFRPIIERELSDPLVTPAAINAAGRRLKELGFEPQLGRAQGATNLFVE
                     TGGRRLLLRAAGGAFVVEDKRFTRADLQALLDADPTAITPAAGLRPITQDFALPTAVF
                     VLGPGELRYVSQLRDVYRFHGVPMPLAWQRATGLGSARHVSGRNGPNC"
     gene            1413..>1689
                     /locus_tag="PKN52_08610"
     CDS             1413..>1689
                     /locus_tag="PKN52_08610"
                     /inference="COORDINATES: protein motif:HMM:NF015962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0050532 - 2-phosphosulfolactate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HNR00050.1"
                     /translation="MLINTDIVPSGPYSDVVVLIDVLRTCTVAPMLFERGARSLSLTP
                     SMRRARAEAGAGRVLVGERQGVPPEGFNHGNSPANLAQVAVAGLDVVM"
BASE COUNT          265 a          572 c          606 g          246 t
ORIGIN      
        1 agcagttcgg tccgttgcgc cctgaaacgt gccgcgctga ccccaagccc gtcgctctct
       61 gccacgccag cggcatgggc acgccgtgga agcggtagac gtcccgcagt tgcgagacgt
      121 agcgcagctc gcccggaccg agcacgaaca ctgcggtggg cagggcgaag tcctgagtga
      181 tggggcgcag gccggcggcg ggagtgatgg cggtggggtc ggcatcgagg agcgcctgga
      241 ggtccgcgcg cgtgaagcgc ttgtcctcga cgacgaaagc gccgcccgcc gccctcagga
      301 gcagccgcct gccgcccgtc tcgacgaaca ggttggtggc gccctgcgcg cgcccgagct
      361 gcggctcgaa gccgagttcc ttcaggcgcc tgcccgccgc gttgatggcg gccggcgtga
      421 cgagcgggtc ggacaactcg cgctcgatga tcgggcggaa gtgctcggcg acgtcgctcc
      481 tgagcgggtc tatcagcacg aggccggcgg ggccgagcaa gcgcgtgaag agcgctgcga
      541 accagtcgct gtacgtggtc accgagctcg ccgcgccctc cagcagctcc cacacgggct
      601 cgaggaagcg cggtcgtggg gtgagcgccg ccagccccgc ggagacaccg tctaccatct
      661 ccctggtaag ggggacgcgg ccgacggcga caccgtccgg cagccggacg cttagcctgc
      721 gcaaggtctc actcgcgtcc agcatgtacg tgttgtccat ctcgtgagcg tcgtggtcct
      781 gcgtcgccag ccagaagatg ggcacgaccg gccgctcccg cgtgtcgagt cgcctggcca
      841 gcgccacggc cgtcatggcc ttcgacaggc tgtaagtagg ccccagcagc aaaccggtct
      901 gctgccccgt gaccacggcg cgcgcctccg gatgcgagag gcgctcgagg ttggcgagca
      961 ccgggggcgg agcgccgagg gcgatggcgt ggcgcttgag agcggctacg agcgacgcgc
     1021 gctcggacgg tcgcggcagg gccagcgcct tgtctacgtc gcctggcgcc acttcaaaga
     1081 agtcgtcgag cgaaccggag gagtaggcgt gcgcgaagtc tgttggtggc gtcgctcgtg
     1141 gtggcacggc gcagcttatc caaggtgagt gcgcgtggtg gcgcttcgga ctacccgcga
     1201 cgccgcgaac cggtctccat gagagccgcg atcggccgcc cggtacttcg tgatacactc
     1261 acgcggccag aggtgggacc gaaaccacct tcccgtaaga agggggaacc gcgagcatcg
     1321 cggcttaacc gggctcatcg gacctcgggc ggccgtatct catgccggcg aacgcgttca
     1381 agagcgcgcc agccgcgcga atcaagacac ttatgttgat caacacggat atcgttccta
     1441 gcggaccata ctcagacgtc gtcgtactca tagacgtgtt gcggacgtgc accgtcgcac
     1501 cgatgctctt cgagcggggc gcccgcagct tgtcgctcac gccctcgatg cgccgcgcgc
     1561 gcgcggaggc cggcgccggg cgcgtgctcg tcggcgagcg ccagggcgtg ccgcccgagg
     1621 gcttcaacca cgggaactcc ccggccaacc tcgcgcaggt cgccgtagcc ggcctcgacg
     1681 tcgtcatgg
//
LOCUS       DAOQYE010000531         1688 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_87203_length_1688_cov_2.278628, whole genome shotgun sequence.
ACCESSION   DAOQYE010000531 DAOQYE010000000
VERSION     DAOQYE010000531.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87203_length_1688_cov_2.278628"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            164..754
                     /locus_tag="PKN52_08615"
     CDS             164..754
                     /locus_tag="PKN52_08615"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNR00051.1"
                     /translation="MVGKSGSGKSTLMHLLALLDTPDDGRIVVEGTDARSLSADALNK
                     LRNSAFGFVFQQFFMIPNASVLENVVLPLKIAGMPRAERTARGMEVLRQVEMQDKARN
                     MATALSGGQKQRMVIARALANEPRIIFADEPTGNLDSATGAVVEDLLFKLNAEHGITL
                     VVVTHDEELAERCDRRVYIKDGRIVDSAATAVEVRR"
     gene            751..>1688
                     /locus_tag="PKN52_08620"
     CDS             751..>1688
                     /locus_tag="PKN52_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00052.1"
                     /translation="MSLTEIVRTAVGNAFRSKLRTTLTVLAIFVGAFTLTLTNGLGTG
                     ITNYIDSQVSSLGATDIITIGQASLAEGGAFGAATSGPTEYDPDKAVVAGQMGPQFAA
                     LTNADLAAILGIDGILSVEPLRMVSPDYIGGPNGKKYEFTVNPSPTGANVALVAGASL
                     GGSSGGGTGTSEGRQLLLPTAYVEPLGFASPAAAVGATVTIGVSDVFGGKHELEAKVA
                     GVQEDTIFAFGMIVSEGLTEALYAAQTTGLPAAAAEIYPVAVARFSPTASTAQVRAIK
                     ASLEEHGYVGQTVADQLGTVNTVINGIVGVLNAFAV"
BASE COUNT          295 a          590 c          567 g          236 t
ORIGIN      
        1 cctctagccg cccgggccgc agcccgggca gaacccgaaa gcgagcgaga ccattcacat
       61 cctggaagca cacgccatca agaagtcgta cggcagcggg gagagctact tcgaggccct
      121 caagggcgtc tcgctctccg tcgccgccgg cgagtccgtc gccatcgtcg ggaagagcgg
      181 ctcgggcaag tcgacgctca tgcacctcct cgcgctcctc gacacgccgg acgacgggcg
      241 gatcgtggtg gagggcaccg acgcgcgcag cctctccgcc gacgcgctca acaagctgcg
      301 caacagcgcg ttcgggttcg tgttccaaca gttcttcatg atccccaacg cgagcgtgct
      361 ggagaacgtc gtgctgcccc tgaagatcgc tggcatgccg cgcgctgagc ggaccgcgcg
      421 cggcatggag gtcctgcggc aggtcgagat gcaggacaag gccaggaaca tggctaccgc
      481 gctgtccggc ggccagaagc agcggatggt catcgcgagg gcgctcgcca acgagccgcg
      541 gatcatcttc gcggacgagc cgacgggcaa cctcgacagc gccacgggcg ccgtcgtcga
      601 ggacctgctc ttcaagctca acgccgagca tgggataacg ctcgtcgtcg tcacgcacga
      661 cgaggagctg gcggagcgct gcgaccgtcg cgtctacatc aaggacggcc gcatcgtcga
      721 tagcgcagcc accgcggttg aggtgcgccg atgagcttga ccgagatcgt caggaccgcc
      781 gtcggcaacg ccttccgcag caagctcagg accacgctga cggtcctggc catcttcgtc
      841 ggcgccttca ccctcaccct gacgaacggc ctgggtaccg gcataaccaa ctacatcgac
      901 tcccaggtca gcagcctggg cgccaccgac atcatcacga taggtcaggc ctcgctggcc
      961 gagggcggcg cgttcggcgc cgcgacgtcc ggcccgaccg agtacgaccc tgacaaggcg
     1021 gtggtggccg gccagatggg gccgcagttc gcagcgttga ccaacgcgga cctggccgcc
     1081 atcctgggca tagacggcat cctgtctgtc gagccactgc gcatggtctc gcccgactac
     1141 atcggcggac ccaacggcaa gaagtacgag ttcaccgtca acccgtcgcc gaccggcgcg
     1201 aacgtggccc tggtcgccgg cgcgagcctg gggggctcgt ccggcggcgg cacgggcacc
     1261 tcggaggggc ggcagttgct cctgccgacc gcctacgtcg agccgcttgg cttcgccagc
     1321 ccggccgcgg cggtcggggc gaccgtcacg atcggcgtga gcgacgtgtt cggcggcaag
     1381 cacgagctcg aagccaaggt agccggagtg caggaagaca cgatcttcgc cttcgggatg
     1441 atcgtcagcg aagggctgac cgaggcgctc tacgcggcgc agacgacggg cctgcccgcc
     1501 gcggcggccg agatctatcc cgtggccgtc gcgcggttct cgcccaccgc cagcaccgcg
     1561 caggtcaggg ccatcaaggc ctcgctggag gagcacggct acgtcgggca gaccgtcgcc
     1621 gaccagctcg gtaccgtcaa caccgtcata aacggcatcg tcggggtgct gaacgccttc
     1681 gcggtcat
//
LOCUS       DAOQYE010000532         1686 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_87362_length_1686_cov_2.720417, whole genome shotgun sequence.
ACCESSION   DAOQYE010000532 DAOQYE010000000
VERSION     DAOQYE010000532.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87362_length_1686_cov_2.720417"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            68..946
                     /locus_tag="PKN52_08625"
     CDS             68..946
                     /locus_tag="PKN52_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNR00053.1"
                     /translation="MTGAGTEHALARGGGVGVWRRALASLARKPFAVASLVIIVAFVL
                     VAVFAPQIAPYDPTKADFLAVRKAPSAAHLLGTDDVGRDVLSRLIYGARASLMAGALS
                     VVIAMVLGVPLGLIAGFYRGAVSELIMRFTDALLSFPFLILAVALAAAFGPSLTNAMI
                     AIGVASAPTFIRLTRGQVLAVRAEEYVQAASALGAGDARVLWSHVLPNSLAPLLVQGT
                     LTIATSIIAESSLSFLGLGVQPPTPSWGGMLNVAKNFMGQAPWMAIWPGLAIFVSVLA
                     FNLLGDGVRDAFDPRD"
     gene            1006..>1686
                     /locus_tag="PKN52_08630"
     CDS             1006..>1686
                     /locus_tag="PKN52_08630"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HNR00054.1"
                     /translation="MRLDYALPYSSQRPVVMARNVVATSQALAAQAGLAMLQKGGNAV
                     DAAVATAITMTILEPTGNGIGSDAFCILWDGRELHGLNASGRSPAAFEAKHLEAAGGG
                     APGSGGGVPRGWNSVTVPGAVSAWVELSGRFGRLPFETLFEPAIRYASDGFPVQPITA
                     RAWGYAEKSFGAPEFAEFRRVFLPGGRAPRAGERFSMPDHARTLELIAKTRGEAFYRG
                     ELAEAMVED"
BASE COUNT          210 a          643 c          575 g          258 t
ORIGIN      
        1 cctacatcgt catcaacctc ctcgtcgacg tcgcttacgc gttggcgaac ccgaagatcc
       61 aggtcggatg acgggcgccg ggaccgagca cgctttggcc cgaggcggcg gggtcggcgt
      121 gtggcgccgc gccctggcca gcctcgcgcg caagccgttc gccgtggcga gcctcgtgat
      181 catcgtggcg ttcgtgctcg tcgccgtctt cgccccccag atcgccccct acgacccgac
      241 gaaggccgac ttcctcgccg tgcgcaaggc gccgagcgcc gcgcacctgc tcggcacgga
      301 cgacgttggc cgcgacgtgc tctcgcgcct catctacggg gcaagggcgt cgctcatggc
      361 cggcgcgctg tcggtcgtca tcgcgatggt cctcggcgtg cccctcgggc tgatcgccgg
      421 cttctaccgg ggcgccgtga gcgagctcat catgcgcttc acggacgcgc tcctctcgtt
      481 cccgttcctg atcctggcgg tggccttggc ggccgcgttc gggccgagcc tgacgaacgc
      541 gatgatcgcg atcggggtgg cgagcgcgcc caccttcatc cgcctgacgc gcggccaggt
      601 cctcgccgtg cgcgccgagg agtacgtcca ggccgccagc gcgctcgggg cgggcgacgc
      661 gcgcgtgctg tggtcgcacg tgctgcccaa cagcctcgcg ccgctcctcg tgcagggcac
      721 gttgacgatc gccacgtcca tcatcgcgga atcttcgtta tcgttccttg ggctgggcgt
      781 gcaaccgccc acgccgagct ggggcggcat gctcaacgtc gccaagaact tcatggggca
      841 ggccccttgg atggccatct ggcccggcct ggccatcttc gtcagcgtcc tcgccttcaa
      901 cctcctcggc gacggggtca gggacgcgtt cgacccccgc gactgacccg gggcgcccac
      961 gcgggccgcg caggcccgcc gcaccaggaa ggaccacgcc ttaacatgcg gctcgactac
     1021 gcactcccct actcctcgca acggccggtg gtgatggcgc gcaacgtcgt cgccacgtcg
     1081 caggcgctcg ccgcccaggc gggcctcgcc atgctgcaga agggcggcaa cgccgtcgac
     1141 gccgccgtgg caaccgccat caccatgacg atcctcgagc cgaccggcaa cggcatcggc
     1201 tccgacgcct tctgcatcct gtgggacggc cgggaactac acggcctcaa cgcctccggg
     1261 cgctcgccgg cggccttcga ggcgaagcac ctggaggccg cgggcggcgg cgcgccgggg
     1321 tccggcggcg gcgtgccgcg cggctggaac tccgtgaccg tgccgggcgc cgtttccgcc
     1381 tgggtcgagc tctccgggcg cttcgggcgc ctgccgttcg agacgctctt cgagccagcc
     1441 atccggtacg cgtctgacgg cttccccgtc cagccgatca cggcgcgcgc gtgggggtac
     1501 gccgagaagt ccttcggcgc gcccgagttc gccgagttcc gccgcgtctt cctgcccggc
     1561 ggccgcgcac cccgcgcggg cgagcggttc agcatgcccg accacgcgcg caccctcgag
     1621 ctgatcgcca agacgcgcgg cgaggccttc taccggggcg agctcgccga ggcaatggtc
     1681 gaggac
//
LOCUS       DAOQYE010000533         1680 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_87921_length_1680_cov_2.384000, whole genome shotgun sequence.
ACCESSION   DAOQYE010000533 DAOQYE010000000
VERSION     DAOQYE010000533.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87921_length_1680_cov_2.384000"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="PKN52_08635"
     CDS             complement(<1..488)
                     /locus_tag="PKN52_08635"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNR00055.1"
                     /translation="MTVEWLAPFLQNLSGGLLIGGIYALIGVGLSLIFGVMRIINFAH
                     GEFVAVGMYTAIVLFQRLGMDPYVALLVAAPLGFVLGAALQRVVLHRLIDAPADSTLL
                     ATLGLALVLSNVLFLIFGAEPKSIYLPYATATVTLGGVRLPVAQLIAGAITLVVIVGL
                     WL"
     gene            complement(527..>1680)
                     /locus_tag="PKN52_08640"
     CDS             complement(527..>1680)
                     /locus_tag="PKN52_08640"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNR00056.1"
                     /translation="AAAKHALRYALPMMRKAATVHLVSIDEEGAPKSVPSAQQIADYL
                     MEVHCVSVQASGLRAADSPPEQLAEFYAEVGADMLVMGAYSSGITNPLAQYFTRQQRP
                     FLVFTSSDDAITRPGSDWIFRINQPSYAYAQVLFDVFDAINAERGAGTLKTIVTVHGN
                     GAFESAVADAVDLIASERGYTVLQRQDYDQAGADFRPILNRFKTLNPDVLFMVSYAAD
                     SVALMRQVQEVGLDAKVFAGGAAGFALPGFIEGSGPAADYVFTATMWTKDVPYPGAQD
                     LNARLSAILGRTPSYHAAQAYAAVITAVDVLKRAASFAPADVRAALLATNMPDTVYGP
                     IRFEDYQGYKNQAPLPAVAEQVVNGDFVTVYVNGQVVGDLLETPSWNAR"
BASE COUNT          281 a          534 c          617 g          248 t
ORIGIN      
        1 atcagccaga ggcccacgat cacgacgagg gttatggcgc ccgctatcag ctgcgccacc
       61 ggcagccgca ccccgccgag cgtcacggtg gcggtggcgt acggcaggta gatcgacttc
      121 ggctcggcgc cgaagatcag gaacagtacg ttggagagca cgagggccag gccgagcgtg
      181 gccaggagcg tgctgtctgc cggcgcgtcg atcaggcggt gcaggacgac gcgctgcagc
      241 gcggccccga ggacgaagcc cagcggcgcg gccacgagca gcgcgacgta ggggtccatg
      301 ccgaggcgct ggaagagcac gatggccgtg tacatcccga cggccacgaa ctcgccgtgc
      361 gcgaagttga tgatgcgcat gaccccgaag atgagcgaca gccccacgcc gatgagagcg
      421 tagatgccgc ctatcaacag gccgccgctg aggttctgta ggaacggagc cagccactcc
      481 acggtcactg gctccgtcct gaggcgaagt gaggcaagct agcgcgtcag cgcgcgttcc
      541 aagacggggt ttcgagcagg tcccccacga cctgtccgtt cacgtacacg gtcacgaagt
      601 cgccgttcac gacttgctcg gccacggcgg gcagcggggc ctggttcttg tagccctggt
      661 agtcctcgaa ccggatgggg ccgtagaccg tgtccggcat gttcgtcgcg aggagcgcgg
      721 cgcgcacgtc cgccggcgcg aaggaggcgg cgcgcttcaa cacgtcgacg gccgtgatga
      781 ccgcggcgta cgcctgggcg gcgtggtacg agggcgtgcg gcccaggatg gcgctcaggc
      841 gggcgttgag gtcctgcgcg cccgggtacg gcacgtcctt cgtccacatg gtggccgtga
      901 acacgtagtc ggcggccggg ccggagccct cgatgaagcc ggggagcgcg aagccggcgg
      961 cgccgcccgc gaacaccttg gcgtctagac cgacttcctg gacctggcgc atgagggcga
     1021 cggagtcggc cgcgtacgac accatgaaca gcacgtcagg gttcagcgtc ttgaagcggt
     1081 tgaggatcgg acggaagtcg gcgcccgcct ggtcgtagtc ctggcgctgg agcaccgtgt
     1141 agccgcgctc ggaggcgatc aggtcgacgg cgtcggcgac ggcgctctcg aacgcgccgt
     1201 tgccgtgcac cgtgacgatc gtcttgaggg tcccggcgcc gcgctcggcg ttgatggcgt
     1261 cgaagacgtc gaacagcacc tgggcgtagg cgtaggaagg ctggttgatg cggaatatcc
     1321 agtcggagcc gggccgcgtg atggcgtcgt cggaggacgt gaacacgagg aacggccgct
     1381 gctggcgcgt gaagtactgg gccagcgggt tggtgatgcc agaggagtac gcgcccatca
     1441 cgagcatgtc ggcgccgacc tcggcataga actccgcgag ttgttcgggc ggtgagtcgg
     1501 cagcgcgcag gccgctcgcc tgcacgctca cgcagtgcac ttccatcagg taatccgcga
     1561 tctgctgcgc gctcggcacc gacttcggcg caccttcctc gtcgatcgac accaggtgca
     1621 ccgtggccgc cttgcgcatc atcggcagcg cataacggag cgcgtgcttg gcggcggccg
//
LOCUS       DAOQYE010000534         1678 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_88051_length_1678_cov_3.482440, whole genome shotgun sequence.
ACCESSION   DAOQYE010000534 DAOQYE010000000
VERSION     DAOQYE010000534.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88051_length_1678_cov_3.482440"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /gene="tdh"
                     /locus_tag="PKN52_08645"
     CDS             <1..261
                     /gene="tdh"
                     /locus_tag="PKN52_08645"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013343673.1"
                     /note="converts threonine and NAD to
                     1,2-amino-3-oxobutanoate and NADH; functions in threonine
                     catabolism; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HNR00057.1"
                     /translation="GGKVALLGIPAKDIAIDWNKVIFKGLNLQGIYGRQMFETWYKMS
                     NLLQAGLDVTPVITHRFAAEEYQQAFDVMRSGEAGKVILDWS"
     gene            265..1311
                     /locus_tag="PKN52_08650"
     CDS             265..1311
                     /locus_tag="PKN52_08650"
                     /inference="COORDINATES: protein motif:HMM:NF017262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HNR00058.1"
                     /translation="MSVAAALKALAYLGTMLLVGGAVCVVWLAPAMVRGESDPANARR
                     KRLAFAALVALGAAVLAASSVLEVANTLREVLGRVDADLVRRYLASTQHGQVVRERWL
                     EVALVATASIALVSWPSRGEGGRPADSGTARPPGWRLSDYGRLAVGAVAVCSSLLLLY
                     GFARLSHAAAMGGAAPLGADFTHMLAAAVWAGPLGYLALFAPGAGAPREGAPGEGARA
                     VFAAAMRRLSTVGALAVAVLAVTGTYNALGHVTEPAAFATSAYGTALWVKLALFALVV
                     VVAALNRFRKLPRFLAGGPVAPLRRTLRIEAALVGALLLVTGALTTAPLPHSDSGMSD
                     AWTNLLHLIDLLRR"
BASE COUNT          199 a          606 c          603 g          270 t
ORIGIN      
        1 ggcggcaagg tcgcgctcct cggcatcccg gccaaggaca tcgccatcga ctggaacaag
       61 gtgatcttca agggcctcaa cctccagggg atctacggtc gccagatgtt cgagacctgg
      121 tacaagatga gcaacctgct ccaggccggc ctcgacgtca cgcccgtcat cacccaccgc
      181 ttcgctgccg aggagtacca gcaggcgttc gacgtcatgc gctcgggcga ggccggcaag
      241 gtcatcctcg actggagctg aggggtgtcg gtcgcggcgg cgctcaaggc cctggcgtac
      301 ctggggacga tgctgctcgt cggcggcgcc gtgtgcgtag tgtggctggc gccggccatg
      361 gtcaggggcg agagcgaccc ggcgaacgcc aggcgcaagc gcctggcgtt cgccgccctg
      421 gtggcgctcg gagcggcggt cctggcggcc agctccgtgc tggaggtcgc caacactttg
      481 cgcgaggtgc tgggccgggt agacgccgat ctcgtgcgcc ggtacctggc gtcgacgcag
      541 cacggccagg tcgtcaggga gcggtggctg gaggtcgcgc tcgtcgcgac ggccagcatc
      601 gccctggttt cctggccgag ccggggtgag ggcggtcggc ctgccgactc cggaacggct
      661 cgtccgcccg gctggcggct ctcggactac gggcgcctcg cggtcggcgc cgtcgccgtc
      721 tgctcctcgc tcctgctcct ctacggcttc gcgcggttga gccatgcggc ggcgatgggc
      781 ggggccgcgc cgctaggagc ggacttcacg cacatgctgg cggcggccgt ctgggccggc
      841 ccgctcggct acctggcgct gttcgcgcca ggggctggcg cgccccgaga gggcgcgccc
      901 ggggagggcg cgagggcggt gttcgccgcc gccatgcggc gcctgtcgac cgttggcgct
      961 ctcgccgtag ccgtgctggc cgtcaccggc acctacaacg ccctcggtca tgtgacggag
     1021 ccggcggcct tcgcaacctc ggcgtacggg accgcgttgt gggtcaagct cgcgctcttc
     1081 gcgctggtcg tcgtcgtcgc cgccctcaac cgcttccgta agctgccgcg cttcctcgcc
     1141 ggcggcccgg tggcgccgtt gcgccgcacc ctgcggatcg aggccgcgct ggtcggcgcg
     1201 ctcctgctcg tcacgggggc gctgaccacg gcgccgcttc cccattccga ctccggcatg
     1261 agcgacgcct ggacgaacct gctgcacctc atcgacctac tgcggcgctg agcccaggcc
     1321 actcaccggt tcgctccatg accacgcgct tcacgctcgc gccgtggtcg cgcctcatgc
     1381 aggatcatct ccgtgaggtg cgccttacta aggcgccgtg agccctgacc cccgtgcgcc
     1441 gccgaacgag gcctacactg acgccacgcg gcaggccctt ccgaccgcca ccgcgctcgt
     1501 agccccaggt cggggccgct gcgtacggaa ggagggggtc gtggatgttc cgccacctct
     1561 tgttgccgtt gacgggcacg gagttcgacc agccggccat agcccacgcc cgtgtcttgg
     1621 cgcttgcgtc gggcgccacc gtggagttgg tgcgcgtcct cgaagcgccg gcgggcgg
//
LOCUS       DAOQYE010000535         1676 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_88218_length_1676_cov_3.539173, whole genome shotgun sequence.
ACCESSION   DAOQYE010000535 DAOQYE010000000
VERSION     DAOQYE010000535.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88218_length_1676_cov_3.539173"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..599
                     /locus_tag="PKN52_08655"
     CDS             <1..599
                     /locus_tag="PKN52_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00059.1"
                     /translation="IDGVADPTRVVLVGHSHGTVWASLLAMNNLDVTFDSFVSLDAVC
                     WMWWNKHKQYIKETFVDGPWTIPFPLDQGDPCGTLSVPGQKERRNINDVVPANVIYGL
                     EVRTTFRLFSLDPNVLTDDNVNVRINGTYANVWGITATESHSDLGKHYNRSVSWVVAM
                     LESLGIPDHSAYPMSTFVLPEPPEGFTYFGGELTPVAP"
     gene            670..>1676
                     /gene="glpX"
                     /locus_tag="PKN52_08660"
     CDS             670..>1676
                     /gene="glpX"
                     /locus_tag="PKN52_08660"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HNR00060.1"
                     /translation="MTKPTEGELKGADRGKQAGGARVAGEERKPTKSVSRSGGLEAAL
                     VLDTVRVTEQAAIAASRVAGKGDADLVDQAGVDAMRSVLNELPIAGEIVIGEGERDEA
                     PMLYIGEKVGTAGPDDWEVDIAVDPVEGTNITARMINNSVAVIAMAERGGLFQAPDTY
                     MEKLIVGPPAKGRVDLTWPVAANLRGVALSLDRAVDDLTVVILDRPRHEQLVREVREA
                     GARVKLIGDGDVIAALAAAVRGTNVHAVMGIGGAPEGVLAAAALKCLGGEIHGRFRPR
                     NEDERRRLQAAGASEDRVYRTEDLAPGRDIVFSATGITDGDLLRGVKFFWRSLLIGWL
                     LL"
BASE COUNT          278 a          559 c          584 g          255 t
ORIGIN      
        1 ggatcgacgg cgtcgccgac ccgacgaggg tggtcctggt aggtcactcg cacggtacgg
       61 tctgggcgtc gctgttggcc atgaacaacc tggacgtcac gttcgactcc ttcgtctcac
      121 tggacgccgt gtgttggatg tggtggaaca agcacaagca gtacatcaag gagacgttcg
      181 tggacggtcc gtggacgatc ccgttcccgc tcgaccaggg cgacccttgc ggcacgctga
      241 gcgtgcccgg gcagaaggag cggcgaaaca tcaacgatgt cgtgcccgcg aacgtcatct
      301 acggtctcga ggtgcgcacg actttccgcc tcttctcgct cgaccccaac gttctcaccg
      361 acgacaacgt gaacgtgcgc atcaacggca cgtacgcgaa cgtttggggc atcaccgcca
      421 ccgagtcgca ctcggacctc ggcaagcatt acaaccgctc cgtgagctgg gtcgtcgcca
      481 tgctcgaatc gctcggcatc cctgaccact ccgcttaccc gatgtcgact ttcgtgctgc
      541 ccgagccgcc cgaaggcttc acctatttcg gcggcgagct tacgcccgtg gccccctgag
      601 ggccggcggt ctaccgacag tgggggaaaa cgcgtggcgc gctgccatgg tgcgagcgca
      661 tagtggggta tgaccaagcc aacagagggc gagcttaaag gggccgaccg gggtaagcag
      721 gcgggcggcg cgagggtcgc gggcgaggag cggaagccga ccaagagcgt ctctcgtagc
      781 ggaggcctcg aggccgccct cgtgctcgac acggtgaggg tgaccgagca ggccgccatc
      841 gcagccagcc gtgtggcggg gaagggcgat gccgacctcg tcgatcaggc gggcgttgac
      901 gctatgcgct ccgttctcaa cgagctgccc atcgcgggcg agatcgtgat cggcgagggc
      961 gagcgcgacg aggcgcccat gctctacatc ggcgagaagg tgggcacggc cggaccggac
     1021 gactgggagg tcgacatcgc cgtcgacccc gtcgagggca ccaacatcac ggctcgcatg
     1081 atcaacaact ccgtcgccgt gatcgcgatg gccgagcgcg gcggcctctt ccaggcgccg
     1141 gacacctaca tggagaagct catcgttggt ccgccggcca agggcagggt cgacctgacc
     1201 tggcccgtcg cggcgaacct gcgcggcgtc gcgttgtcgc tcgaccgggc cgtcgacgac
     1261 ctgacggtcg tcatcctcga ccgcccgcgc cacgagcagc tcgtgcgcga ggtgcgtgag
     1321 gcgggcgcgc gcgtcaagct catcggcgac ggcgacgtca tcgcggccct ggccgccgct
     1381 gtgcgcggca ccaacgtgca tgcggtcatg ggcatcggcg gagcgccgga aggggtactg
     1441 gcggccgccg cgctcaagtg cctcggcggc gagatccacg gtcgcttccg cccgcgcaac
     1501 gaggacgagc gccggcgcct gcaggcggcc ggcgccagcg aggaccgcgt ctatcgcacg
     1561 gaggacctgg cgccagggcg cgacatcgtc ttcagcgcca cgggcatcac ggacggcgac
     1621 ctcctgcgcg gcgtcaagtt cttctggcgc agcttgctga taggctggct gctcct
//
LOCUS       DAOQYE010000536         1665 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_89229_length_1665_cov_2.610559, whole genome shotgun sequence.
ACCESSION   DAOQYE010000536 DAOQYE010000000
VERSION     DAOQYE010000536.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89229_length_1665_cov_2.610559"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="PKN52_08665"
     CDS             <1..375
                     /locus_tag="PKN52_08665"
                     /inference="COORDINATES: protein motif:HMM:NF017013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOFRL family protein"
                     /protein_id="HNR00061.1"
                     /translation="PRRRARPRGAAASSGRGRATGGPNGEWALAFAAELGRSDYWLLA
                     IDTDGIDGASAAAGALLDPAALGRLTPARIERSLAAHDSHGLLGAVGGLLVTGPTRTN
                     VNALRIVLFPHPHGGGDGTRPA"
     gene            395..1366
                     /locus_tag="PKN52_08670"
     CDS             395..1366
                     /locus_tag="PKN52_08670"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HNR00062.1"
                     /translation="MRVLPPTPDNLSLAAERLAAGRLVAMPTETVYGLAAAARDALAV
                     GRVFELKGRPHDHPLIVHLGPAMDVGEWTVGGSERLASLAAALWPGPLTVVVRKAPWV
                     PDAVTGGQPTVALRVPAHPVALALLRAFGDAVAAPSANRFGRISPTTAEHVAAEFAGS
                     DLVVVDGGPCRIGLESTIVDLTGEVARVLRPGGVPVEVVEAAIGERVELAGGAKTPRV
                     PGSLPSHYAPATPASLLDAVELEARANACPGVGVLARRPAPGWFGGMWVELPDDPVAY
                     GAGLYAALRRLDAAGPSEILIEHVPGAATWLAVRDRLERATARGRGA"
     gene            1377..>1665
                     /locus_tag="PKN52_08675"
     CDS             1377..>1665
                     /locus_tag="PKN52_08675"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034023 - 5-(carboxyamino)imidazole
                     ribonucleotide mutase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HNR00063.1"
                     /translation="MSNPETGSGWTPLVAVVMGSTSDWETLRQAAEVLERLEVPHVCK
                     VVSAHRTPERLSEFAKGAATRGLEVIVAGAGGAAHLPGMIAANTHLPVLGVP"
BASE COUNT          197 a          623 c          621 g          224 t
ORIGIN      
        1 cctcgtcggc gagcacgtcc tcgaggagcc gccgcgtcgt cgggtcgcgg tcgggctact
       61 ggcgggccga acggggagtg ggcgttggcg ttcgcggccg agctcggacg cagcgactac
      121 tggctcctgg ccatcgacac cgacggcata gacggcgcct cggcggcggc gggcgcgctc
      181 ctcgacccgg cggccctcgg gcgcctcacg ccggcacgga tcgagcgctc gctggccgcc
      241 cacgattccc acggcctgct aggcgccgtc ggcggcctcc tcgtgaccgg tcccacccgc
      301 acgaacgtca acgctctgcg catcgtcctg ttcccgcacc cccatggtgg cggcgacggc
      361 acccggccgg cttgagtggg tatggtagtc gccagtgcga gtattgccgc ccacgccgga
      421 caacctcagc ctcgccgcgg aacggctggc cgccggccga ctcgtggcca tgccgaccga
      481 gaccgtctac ggtctcgcgg cggctgcgcg cgacgcgctg gcggtggggc gggtcttcga
      541 gttgaagggg cgaccgcacg accacccgct catcgttcac ctcgggccgg ccatggacgt
      601 aggtgagtgg acggtcggcg ggtccgagcg cctcgcgtcc ctggcggcgg cgctgtggcc
      661 gggaccgctc acggtcgtcg tgcgcaaggc gccctgggtg ccggacgccg tcaccggcgg
      721 ccaaccgacg gtggcgctac gcgtccccgc gcacccggtc gcgttggccc tgctccgcgc
      781 cttcggcgac gccgtggccg cgccgtcggc caaccgcttc ggccgcatca gcccgaccac
      841 ggcggagcac gtcgccgccg agttcgccgg ctctgacctg gtggtcgtcg acggaggccc
      901 gtgccggatc ggcctcgagt cgaccatcgt cgacctgacc ggcgaggtgg cgcgggtgct
      961 gcgtccgggc ggcgtacccg ttgaggtggt cgaggcggcc atcggcgagc gcgtcgaact
     1021 cgcgggtggc gccaagacgc cgcgcgtgcc aggtagcctc cccagtcact acgcccccgc
     1081 gacgccggcc agcctcctgg acgcggtcga gttggaggcg cgcgcgaacg cgtgccccgg
     1141 ggtcggcgtg ctcgcccggc gcccggcgcc aggctggttc ggtggcatgt gggtggaact
     1201 ccccgacgac ccggttgcct acggcgccgg actctacgcg gcgctgcgcc ggctcgacgc
     1261 cgccggaccg tccgagatcc tcatagaaca cgtgcccggc gccgccacct ggttggcggt
     1321 ccgcgaccgc ctggagcgcg cgaccgcgcg ggggcgcggc gcatgagccg gggcgcatga
     1381 gcaaccccga aaccggcagc ggctggaccc cccttgtcgc ggtcgtgatg ggctccacga
     1441 gcgactggga gaccttgcgc caggccgccg aggtcctgga gcgcctcgag gtcccgcacg
     1501 tatgcaaggt cgtctctgcc caccgcaccc ccgagcgcct tagcgagttc gcgaagggcg
     1561 cggcgacgcg agggctcgag gtcatcgtgg cgggcgcggg cggggcggcc cacctgccgg
     1621 ggatgatcgc cgctaacact cacctgccag tcctaggagt gccgg
//
LOCUS       DAOQYE010000537         1664 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_89296_length_1664_cov_2.968303, whole genome shotgun sequence.
ACCESSION   DAOQYE010000537 DAOQYE010000000
VERSION     DAOQYE010000537.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89296_length_1664_cov_2.968303"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            81..431
                     /locus_tag="PKN52_08680"
     CDS             81..431
                     /locus_tag="PKN52_08680"
                     /inference="COORDINATES: protein motif:HMM:NF014132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HNR00064.1"
                     /translation="MNAKDLLMRVPETVRPPDGVRPTKRTVVQYSISEPVYHVLENGE
                     VVAVEGTAPDPDVTIKVGDDDLIDLLGGRLSPAAALFTGRLKVRGDIGLAQRLIGLVD
                     REKLAAVQNELERA"
     gene            complement(433..897)
                     /locus_tag="PKN52_08685"
     CDS             complement(433..897)
                     /locus_tag="PKN52_08685"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HNR00065.1"
                     /translation="MGVRLTTLAYVRSGEKTLMLRRGRAYSGEHGQWNGLGGKLEAGE
                     TPEECMRREVMEESGLEVLEASYKGLLTFPGFDGDTDVLVFVFVVTSFAGEPRASSEG
                     ELFWIDTAGLSALDLWEGDRHFLPWLDEPGTFSAKFVYVDGRYVGHDVVRYP"
     gene            complement(952..1263)
                     /locus_tag="PKN52_08690"
     CDS             complement(952..1263)
                     /locus_tag="PKN52_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00066.1"
                     /translation="MRADRLEQVFLRPSAGRLQALVVLESEAGARERVTLSPPTAGIG
                     PAAELAAARYVAKWLWSNGGRTADLVRVRRERGDALEEAREARSELLAELQRLAGRRE
                     A"
     gene            complement(1279..>1664)
                     /locus_tag="PKN52_08695"
     CDS             complement(1279..>1664)
                     /locus_tag="PKN52_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HNR00067.1"
                     /translation="DVSVDMIIQGVRGASDSRQQMAFIVGKDVVPDALAALEPVLGEM
                     GAHAEVDTDVAKVSIVGIAIGSTPGVAARMFDAVAAANANIDMITTSEVRISVLIPAE
                     RAQAVLEAVHTAFGLDAADGAAADD"
BASE COUNT          250 a          612 c          564 g          238 t
ORIGIN      
        1 cgaagtccaa gggcctctct cctcaagtgg cgagtatagc caaggccgcg gcgccgacct
       61 tcggccgcta gactggacgc atgaacgcca aggacctgct catgcgcgtg ccggagaccg
      121 tgcggccgcc ggacggcgtg cgccccacta agcgcaccgt ggtgcagtac tccatctcgg
      181 agcccgtcta ccacgtcctg gagaacggcg aggtggtcgc tgtcgagggc acggcgcccg
      241 acccagacgt caccatcaag gtaggcgacg acgacctgat cgacctgctg ggcggtcggt
      301 tgagcccggc cgcggcactg ttcacgggtc gtctcaaggt acgtggcgac atcgggctgg
      361 cgcagcgcct catcggcctg gtagaccggg agaagctcgc ggccgtccag aacgagctcg
      421 agcgggcctg actcacggat agcgaacgac gtcgtggccg acgtagcggc cgtccacgta
      481 cacgaacttg gccgagaacg tgcccggctc gtcgagccac ggcaggaagt gcctatcgcc
      541 ctcccagagg tcgagggcgc tcagtccggc cgtgtcgatc cagaagagct cgccctcgct
      601 cgaggcgcgc ggctctcccg cgaagctcgt gaccacgaag acgaacacca ggacatcggt
      661 gtcgccatcg aaacccggga aggtgagcaa ccccttgtag ctcgcctcca gtacctcgag
      721 gccggactcc tccatgacct ccctgcgcat gcactcctcg ggcgtctcgc ccgcctccag
      781 cttgccgccc aggccgttcc actggccgtg ctcgccgctg tatgcccgac ccctgcgcag
      841 catcagggtc ttctcgccgc tgcgcacgta cgccagcgtg gtgagccgta cgcccatggc
      901 gcgcagccta acgccgcgag gcggcggtca gggggacccg gcgtcgcgag ctcacgcctc
      961 gcgccttccc gccagccgct gcagctcggc gagcagctcg gagcgcgcct cgcgcgcctc
     1021 ctctagcgcg tcgccccgct cacgccgcac ccgaacgagg tcggcggtcc tgccaccgtt
     1081 gctccatagc cacttggcca cgtagcgcgc cgcggccagc tcggcggcgg ggccgatccc
     1141 ggccgtcggc ggcgagagcg tgacgcgttc tcgcgccccg gcctcagact cgagcaccac
     1201 cagcgcctgc aaccgtccgg cgctcggccg gaggaacacc tgctcaaggc ggtcggcgcg
     1261 cacgctggcc cgacgcgcct agtcgtccgc ggcggcgccg tccgcggcgt cgaggccgaa
     1321 cgccgtatgc acggcctcga ggacggcctg cgcccgctcg gcggggatga gcacgctgat
     1381 gcgcacctcg ctcgtggtga tcatgtcgat gttggcgttc gccgcggcga cggcgtcgaa
     1441 catgcgggcg gcaacgccgg gggtcgatcc gatggcgatg ccgacgatcg agaccttggc
     1501 gacgtccgtg tcgacctcgg cgtgcgcgcc catctcgccg agcacgggct ccagggccgc
     1561 gagggcgtcg gggaccacgt ccttgccgac gatgaaagcc atctgttggc ggctgtcaga
     1621 ggcgccgcgg acgccctgga tgatcatgtc gacgctcacg tcgg
//
LOCUS       DAOQYE010000538         1662 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_89493_length_1662_cov_2.171749, whole genome shotgun sequence.
ACCESSION   DAOQYE010000538 DAOQYE010000000
VERSION     DAOQYE010000538.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89493_length_1662_cov_2.171749"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            290..1618
                     /gene="aspS"
                     /locus_tag="PKN52_08700"
     CDS             290..1618
                     /gene="aspS"
                     /locus_tag="PKN52_08700"
                     /EC_number="6.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA(Asn) ligase"
                     /protein_id="HNR00068.1"
                     /translation="MSGNAEAVGEGETHKGALEARVLVRDLAAHVGRRVDVAGWILTR
                     RDLGGIRFLVLRDRTGVVQCVLEGIDVPLHESCVRVSGKVVEQPRAPGGLEVQASALE
                     VISAATVPPPVELSKEEWQANPDTLLQYRHVTVRSPKARAVLKIEAELIRGFRAYLDA
                     HDFTEIYTPKLVSAGAEGGANLFEVDYYGRRAYLAQSPQLYKQIMVGVFERVYETAPV
                     YRAEKSHTHRHLSEYLSLDVEFGFIEDDGDVMDLEEALLKSMLNGVAESCSRDLAALG
                     AELPDTSVAFPRIELLAARELVAERYGHVSGGKDLDPEAERLVGRWAKEEHGSDFVFV
                     THYPQAARPFYTFPLDGGLTRGLDLLFRGVEITSGGQRVHRPEVLVEELRKRNMDPEA
                     FADYIEVFRHGMPPHGGFAIGAERLTALLLGIHNVRFARAFPRDAQRLTP"
BASE COUNT          266 a          582 c          567 g          247 t
ORIGIN      
        1 cgtcatcgag gtgggcgtgc aggtagagct ctgacggagg ccgcaggttg actccccctt
       61 accctcctgc taagcttccg cgcatgacga acgcgctcgc cctgcgcacg gacgataatt
      121 cggattcgag gctactcggg gcgtaggcgt cgtaacggct cactgcactc acccccgagg
      181 ccctagaccc tcgggggttt ctcgttatgg gctcgaagcc gagcccgaac ggccgccacc
      241 aaggcgaagc agcgatccgg gaagcagcaa ggcccaagga ggcggtcgga tgtctggcaa
      301 cgcagaagcg gtcggagaag gcgagacgca taagggcgcg ctggaggcgc gggtgctggt
      361 acgcgacctg gccgcgcatg tgggccggcg cgtcgacgtg gctggctgga tcctcactcg
      421 ccgcgacctc ggcggcatcc gcttcctcgt actgcgcgac cgcaccggcg tcgtccagtg
      481 cgtgttggag ggcatcgacg tgcccctgca cgagagctgc gtacgggtga gcggcaaggt
      541 ggtcgagcag ccgcgggcgc cgggcggcct cgaagtacag gcttccgcgc tcgaggtcat
      601 cagcgctgcc accgtcccgc cgccggtcga gctctccaag gaggagtggc aggccaaccc
      661 ggatacgctc ctgcagtacc gccacgtgac cgtgcgcagc ccgaaggcgc gcgccgtcct
      721 caagatcgag gcggaactga tccgcggttt cagggcgtac ctggacgcgc acgacttcac
      781 ggagatctac acgcccaagc tcgtgtcggc gggtgccgag ggcggcgcca acctgttcga
      841 ggtcgactac tacgggcgcc gcgcctacct cgcccagtcg ccgcagctct acaagcagat
      901 catggtcggc gtcttcgagc gcgtgtacga gaccgccccc gtctaccgcg ccgagaagag
      961 ccacacgcat cgccacctct ccgagtacct gtcgctcgac gtcgagttcg gcttcatcga
     1021 ggacgacggc gacgtcatgg acctcgagga ggcgctcctc aagagcatgt tgaacggcgt
     1081 ggcggagagc tgcagccggg acctcgccgc gctcggcgcc gagctgcccg acacgagcgt
     1141 ggccttcccg cgcatcgagc tgctggcggc gcgcgagctc gtcgcggaac ggtacgggca
     1201 cgtctcgggc ggcaaggacc tcgaccccga ggccgagcgc ctcgtcggcc gctgggccaa
     1261 ggaggagcac ggctcggact tcgtgttcgt gacccactac ccgcaagcgg ccaggccgtt
     1321 ctacacgttc ccgctcgacg gcggtctgac ccgcgggctc gacctgctct tcaggggggt
     1381 ggagatcacg tcgggcgggc agcgcgtgca ccgacccgag gtcctcgtcg aggaactccg
     1441 caaacgcaac atggaccccg aggccttcgc cgactacatc gaggtattcc ggcacggcat
     1501 gccgccgcac ggcggcttcg ccatcggcgc ggagcgcctc accgccctgc tgctcggcat
     1561 ccataacgtg cgcttcgcgc gcgccttccc ccgcgatgcc cagcgcttga cgccttgagc
     1621 cttgcgctaa gcgcgagtcg cggcacgagc ccgggccgtc gt
//
LOCUS       DAOQYE010000539         1660 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_89611_length_1660_cov_2.962617, whole genome shotgun sequence.
ACCESSION   DAOQYE010000539 DAOQYE010000000
VERSION     DAOQYE010000539.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1660
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89611_length_1660_cov_2.962617"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="PKN52_08705"
     CDS             <1..274
                     /locus_tag="PKN52_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNR00069.1"
                     /translation="LHMLPNALGPILVSTTLSVGGAILLESALSFFGFGVQPPTPTWG
                     NLLNSSRDWLDIAPWIATFPGLFILVTVLAVNFLGDGLRDAFDPRL"
     gene            330..1427
                     /locus_tag="PKN52_08710"
     CDS             330..1427
                     /locus_tag="PKN52_08710"
                     /inference="COORDINATES: protein motif:HMM:NF014601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase N-terminal
                     domain-containing protein"
                     /protein_id="HNR00070.1"
                     /translation="MKILIVGSGAIGGLAGAHMTRAGYDVTLVDRWREHVEAMNANGL
                     RFDGIRGEFAIPVTAITPDELPEHVATRGPLEAVLIATKSQHTEDAVRMLMPLMGGET
                     FIVSYQNGFNEPMITDLLAKAGIGGEERVMGSIPNYGGALVDPGHIEFVHEGPIQLGE
                     MTRRRTPRLAELAEALSSLTEVQLSDNIWGQIWAKEVYSQQVVFSALADARIHETLGV
                     ERYARVAGAVVREALEIAEACGITVEAFDFFDPANYKPATASDTRRLIDNINHAIWLL
                     KKDQKPSEHVFKKQGSGIWWDIVYRKRASEVGSMSGALVEYGKGAGADTRLVEKLGNM
                     ILEIEAGDRELGFHNYDELEGYVRSIGKELP"
     gene            1424..>1660
                     /locus_tag="PKN52_08715"
     CDS             1424..>1660
                     /locus_tag="PKN52_08715"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNR00071.1"
                     /translation="MNEKRLGVGVLGAHAWAEKAHLPGYAAYDRARLVAICDVVPERA
                     KALAEQFGVQRVYSDPAELIADPEVELVDVCTPTD"
BASE COUNT          305 a          562 c          535 g          258 t
ORIGIN      
        1 cctccacatg ctccccaacg cactcggacc gatcctggtg agcacgaccc tgtcggtcgg
       61 aggcgcgatc ctcctcgagt cggcgctctc cttcttcggc ttcggcgtgc agcccccgac
      121 gccgacctgg ggcaacctcc tcaactcgtc gcgggattgg ctcgacatcg caccgtggat
      181 agccaccttc cccggcctgt tcatcctagt gaccgtccta gccgtcaact tccttggcga
      241 cggcctgcgc gacgcgttcg atccacgtct atgagccctc aagcgcgttc tctccgccca
      301 gacgcggcgg cgagacggaa ggagtaccgg tgaagatact gatcgttggt tccggcgcga
      361 taggcgggct ggccggcgcg cacatgacgc gggccggcta cgacgtcacc ctcgtcgaca
      421 ggtggcggga gcacgtcgag gccatgaacg ccaacggact caggttcgac ggcatccgcg
      481 gcgagttcgc catcccggtc acggccatca cccctgacga gcttcccgag cacgtggcca
      541 cccgcggtcc tctcgaggcc gtgctgatag ccaccaaatc ccagcacacc gaggacgccg
      601 tgaggatgct catgccgctg atgggcggcg agacgttcat cgtctcctac cagaacggtt
      661 tcaacgagcc gatgatcacc gacctccttg ccaaggcggg gatcggcggc gaagagcggg
      721 tgatggggtc gatcccgaac tacggcggcg ccctggtcga cccgggccat atcgagttcg
      781 tccacgaggg ccccatccag ctcggcgaga tgacaaggcg acggacgccg cgactagcgg
      841 aactggcaga ggcgctgtcc tcgctgaccg aggtccagct ttccgacaac atctggggac
      901 agatctgggc gaaggaagtc tactcgcagc aggtcgtctt cagcgcgctg gcggacgccc
      961 gcatccacga gacgctcggc gtcgagcggt acgcccgcgt ggccggagcg gtggtcaggg
     1021 aggctctcga gatcgctgag gcctgcggca taacggtcga ggccttcgac ttcttcgacc
     1081 cggcgaacta caagcccgcc acagcctccg acacccgtcg cctgatcgac aacatcaacc
     1141 acgccatctg gctcctgaag aaggaccaga agccgtccga gcacgtgttc aagaaacaag
     1201 gatcggggat ctggtgggac atcgtctacc ggaagcgcgc ctccgaggtg ggctccatga
     1261 gcggagccct ggtcgagtac gggaagggcg ccggggccga tacgcgcctg gtggagaagc
     1321 tcggcaacat gatcctggag atcgaggcgg gcgaccgtga gctcggcttc cataactacg
     1381 acgagctgga aggctacgtc cgcagcatcg gcaaggagct gccatgaacg agaagagact
     1441 cggagtcggc gtcctcggcg cccacgcctg ggcagagaaa gcgcacctgc ccggctacgc
     1501 cgcctacgac cgggcccggt tggtcgcgat ctgcgacgtt gtgcccgagc gcgccaaggc
     1561 gttggcggag cagttcggcg tccaacgcgt ctactccgac ccagccgagc tgatcgccga
     1621 ccccgaagtg gaactggtcg acgtctgcac gcccactgac
//
LOCUS       DAOQYE010000540         1657 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_89885_length_1657_cov_2.551186, whole genome shotgun sequence.
ACCESSION   DAOQYE010000540 DAOQYE010000000
VERSION     DAOQYE010000540.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89885_length_1657_cov_2.551186"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="PKN52_08720"
     CDS             complement(<1..410)
                     /locus_tag="PKN52_08720"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNR00072.1"
                     /translation="MTISAAAHAEKTAVVTAEERVTYSDLHGRALRFASLLQAVGVRR
                     GDRVAIFLENSVDTVAGIYGALYAGAAFMVVNPQTKEDKLHYMLADSGAAAVLSEGRL
                     ARLIAGLMTDPATVLPNLLALVLAGGGEPVSPAAG"
     gene            complement(523..>1657)
                     /locus_tag="PKN52_08725"
     CDS             complement(523..>1657)
                     /locus_tag="PKN52_08725"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="asparagine synthase C-terminal domain-containing
                     protein"
                     /protein_id="HNR00073.1"
                     /translation="FEDPRFDESAFQRRMADELGVNLRTIRVSDADVAAAFPEVVRFA
                     EKPMLRTAPAPLILLSHSVRDAGFKVVLTGEGSDELLGGYNLFQEAMVRRFWARRPES
                     KLRPRLLGKLYPYLSRDLQQGGGFMAEFFKAGMLDLDDPLYSHRPRFRTTGRNLRFLG
                     QETLSTREQVGDPEARVIDRLPAEYAAMGPLGQAQYLEITTFLTGFLLHSQGDRMLAA
                     NSVEGRFPFLDVHVAEFAAGLPERLRLRDLQEKYLLRKSLAKVLPDEINKRPKRPYRA
                     PILRAFFGPGAPAYVDELLAPERLAATGLFNVANVTKLAEKARRYADVGFSESDEMGL
                     VGVLSTMLLDETTVTSPRLAPAAVPTRVVEGSRVKLDGGVRVS"
BASE COUNT          262 a          569 c          580 g          246 t
ORIGIN      
        1 cccgccgcgg ggctcacggg ctcgccgccg ccagccagca cgagcgccag gaggttcgga
       61 aggaccgtcg ccgggtcggt catcaacccg gcgatcaggc gggccagccg cccctcgctc
      121 agcacggccg ccgcgccgga gtcggcgagc atgtagtgga gcttgtcttc cttggtctgg
      181 gggttgacga ccatgaacgc tgcgccggcg tagagcgcgc cgtagatccc ggcgaccgtg
      241 tcgaccgagt tctcgaggaa gatggcgacc ctgtcgccgc ggcgcacgcc gaccgcctgc
      301 agtaggctcg caaagcgtag cgcccgaccg tgcaggtcgg agtaggtgac gcgctcttca
      361 gcggtgacga ccgccgtctt ctccgcatgt gcggcggcgc tgatggtcaa gctgtcttga
      421 agcagccttg gatacctggg ttgcatcggc ctcgcccttc cagtcggcgt gcccaccaag
      481 cggcagccga ccccttccat agccgagtct atggacggac tctcacgaga cccttacacc
      541 accatccaac ttcactcgag acccctcgac cacgcgggtc ggcacggcag ccggcgctaa
      601 ccgcgggctg gtgaccgtcg tctcgtccag cagcatggtg gagagtacgc cgaccaagcc
      661 catctcgtcg ctctcgctga agccgacgtc ggcgtaacgc cgcgccttct cggcgagctt
      721 cgtgacgttg gcgacgttga ataggccggt ggcggccaag cgctccgggg ccaggagctc
      781 atccacgtac gccggcgccc ccggcccgaa gaaggcgcgc aggatgggcg cccggtaggg
      841 gcgcttgggg cgcttgttaa tctcgtcggg cagcaccttg gcgagcgact tgcggagcag
      901 atacttctcc tgcaggtcgc ggagccgcaa ccgctccggc agaccggcgg cgaactccgc
      961 gacgtgcaca tccaggaacg gaaaacgccc ctcgaccgag ttggccgcga gcatgcggtc
     1021 gccctgcgag tgcagcagga agccggtaag gaaggtggtg atctccaggt actgcgcctg
     1081 gccgagcggt cccatggccg cgtactcggc cggcaggcgg tcgatgacgc gcgcctccgg
     1141 gtcgccgacc tgctccctgg ttgacagggt ctcctggccg aggaaccgca ggttgcgccc
     1201 cgtggtcctg aagcgcgggc ggtggctgta gagggggtcg tccaggtcga gcatgccggc
     1261 cttgaagaac tcggccatga acccgccgcc ctgctgcagg tcgcgggaga ggtacgggta
     1321 gagcttgccg aggagccgcg gcctgagctt cgactccggc cggcgcgccc agaagcgccg
     1381 caccatggcc tcctggaaga ggttgtagcc gccgaggagc tcgtccgagc cctcgccggt
     1441 cagcaccacc ttgaagccgg cgtcgcgcac ggaatgcgag agcaggatga ggggcgccgg
     1501 cgccgtgcgg agcatgggct tctccgcgaa gcggacgacc tccgggaacg ccgcggccac
     1561 gtcggcatcc gagaccctga tggtccgcag gttcactccc agctcgtcgg ccatgcgccg
     1621 ctggaaggcg gactcgtcga agcgcgggtc ctcgaac
//
LOCUS       DAOQYE010000541         1655 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_90021_length_1655_cov_3.409375, whole genome shotgun sequence.
ACCESSION   DAOQYE010000541 DAOQYE010000000
VERSION     DAOQYE010000541.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90021_length_1655_cov_3.409375"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(200..1084)
                     /locus_tag="PKN52_08730"
     CDS             complement(200..1084)
                     /locus_tag="PKN52_08730"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNR00074.1"
                     /translation="MGDGGHSGHRRVVAVTGASTGIGRAVVSRLLESGFTVYAGVRRE
                     EDGVSLRKEADERFGDSGALRILYLDVTDEAQVAAAAERVNAELDGGHFHGVVNNAGI
                     AVAGPMEFLPLEDLRRQLDVNVVGQVAVAQAFLPLVRERPGRLVFVGSVSGLISSRLL
                     GAYAASKFALEAVADAFRRELAPHKVKVSVVEPGRIATPIWEKSLGEGLARMDEMVPK
                     AREYYGWLVKDLAAGAAFAARHGTRPEAVAHAVYRALTERRMRTRYFVGSDAHFINVL
                     RRVLSDPILDRVVNSSGR"
BASE COUNT          258 a          617 c          538 g          242 t
ORIGIN      
        1 accgagaaga cggcggcggc gaggcacgca acggtggttc tacgcatcag tcctccgact
       61 tgcaagcagc gccacgctgg cgcggctcca gttaggcggc aggcactggc ccggagtcgc
      121 tcgaggcccc tgccgaagcg ttcgtatggc gaaggttagc acctgcggcg accagccgcc
      181 atgccgccat ggcggcccgt cagcgaccgg aggagttgac gaccctatcg aggatcgggt
      241 cggacagcac ccgcctgagg acgttgatga agtgcgcgtc cgagccgacg aagtagcggg
      301 tccgcatccg gcgctccgtc agggctcggt acacggcgtg cgccaccgcc tccgggcgcg
      361 taccgtgcct tgcggcgaag gcggccccgg ccgcgaggtc cttgacgagc caaccgtagt
      421 actcgcgcgc cttcggcacc atctcgtcca tgcgcgcgag gccctccccg agcgacttct
      481 cccagatggg cgtggcgatg cggccgggct cgacgacgga caccttgacc ttgtgggggg
      541 cgagctcgcg cctgaaggcg tcggcgacgg cctccagcgc gaacttcgac gccgcgtacg
      601 cgccgaggag ccgcgaggag atgaggccgg agacggagcc cacgaacacg agccgtcccg
      661 gccgctcgcg cacgagcggg aggaacgcct gcgcgacggc cacctgaccg acgacgttca
      721 cgtcgagctg gcgtctgagg tcctcgagcg gcaggaactc catgggaccc gccaccgcta
      781 tcccggcgtt gttgacgaca ccgtggaagt ggcccccgtc gagctcggcg ttcacccgct
      841 cggcggcggc cgcgacctgg gcctcgtccg ttacgtcgag gtagaggatc ctcagcgctc
      901 ccgagtcgcc gaagcgctca tccgcctcct tgcgcaacga cacgccgtct tcctcacggc
      961 gcacgcccgc gtacaccgtg aacccgcttt cgagcaggcg agaaaccacg gcccggccga
     1021 tgccggtgga ggctccggtg acggccacca cacgcctgtg gccggagtga ccaccatctc
     1081 ccacgtgcgt atcctaactg ttgcataaaa aacgagaacg ggcttccggg tccgtggcgc
     1141 cgggtccttc ccggcactgc ttccgaagag cggcacgtat gccacgtaga cccggaagct
     1201 ccccagcggt ctatcaggac tcgagcttgg ggccgcgcct tctcagcccg ggcgctacca
     1261 tcgtccgccg tctggcccgg ggtgaggaac cccttcggtc accggcctcg gcccgcttgg
     1321 cacgtgcctc gagcccctac cgcttcgcca tcaggctaag ccgggccgcg gtggccgacc
     1381 agggacctaa gtcctccatg ctcgctcacc cgtcagttcc atgcccatgc cggcgtggcc
     1441 gcgtcaggcc cggccgctcg cgggcatgac cgttcccgac gagccctacc cctgccagct
     1501 ctcgaacagc tcccgcatct cctgcggcac gcggacgggc cggttcgtct ggtagtcgaa
     1561 cgtgacggcc accgcctcca cctccgccgc gacctcacca ccggcgagca ctacctgacg
     1621 catcgtcagg ctggtgttgc cgatgcgctc caccc
//
LOCUS       DAOQYE010000542         1654 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_90122_length_1654_cov_2.825516, whole genome shotgun sequence.
ACCESSION   DAOQYE010000542 DAOQYE010000000
VERSION     DAOQYE010000542.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90122_length_1654_cov_2.825516"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(164..517)
                     /locus_tag="PKN52_08735"
     CDS             complement(164..517)
                     /locus_tag="PKN52_08735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00075.1"
                     /translation="MASASIKRSSDVDNEALLSLFGIILGSIGSLMIGVFWAMGANLK
                     ATANGGTIVQQQLTGLWNTLFWAYRFVVIGAIIVAVGLYAIKRYKEAAGVAALPIIGV
                     VLYYFALVQFHVGPR"
     gene            710..>1654
                     /locus_tag="PKN52_08740"
     CDS             710..>1654
                     /locus_tag="PKN52_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HNR00076.1"
                     /translation="MQDLVIRGARAHNLKNVDLVLPRNKFIVFTGVSGSGKSTLAFDT
                     IYAEGQRRYVESLSAYARQFLGIMDKPDVDSIDGLSPAISIDQKTTSHNPRSTVGTVT
                     EVHDYLRLLFARVGTPHCPVCGRVIQRQSATEIVDRLIERFSGKRGMLLAPVVRGRKG
                     EYRKLFLDLKKEGFARVRVDGVVQTIEQALEADLERFEKHDIDVVIDRVVIDENDRSR
                     IAESAELALTKGEGLLRAFLPDDNVDELFSEKFACPEHGTFLEELEPRTFSFNAPYGA
                     CSHCSGLGYLQQFDPELIIPDPTLSVAQGAIAPWTGARG"
BASE COUNT          291 a          577 c          517 g          269 t
ORIGIN      
        1 gtgaccagga tcgcctcggg ggctagttcg tccagtttca cgcccgcgcg cgggttctgg
       61 gtcacgaacg ggtcgaacac gacctcgtgg ccgtcgccct tcgcctgcac ggcggagtgg
      121 ccaaggtaac gcagctgcat gccgccgacc tccccttacg tgactagcgc ggacctacgt
      181 ggaactggac gagcgcgaaa tagtagagga cgacaccgat gatcggcagt gcggcgactc
      241 cggcggcctc cttgtagcgc ttgatggcgt acaggccgac ggcgacgatg atggcgccga
      301 tcactacgaa ccggtacgcc cagaacaacg tgttccatag gccggtcagc tgctgctgga
      361 ctatggtgcc gccgttggcg gtcgctttca ggttggcgcc catcgcccag aacacgccga
      421 tcatgaggct gccgatggac ccgaggatga tgccgaacaa gctcagcagc gcctcgttgt
      481 ccacgtcgga tgaacgtttg atgctcgcac ttgccacttc aagcctccta taaagctggc
      541 cttaagctta gcagcaagcg cccgtccggg ccccaccgca tgcgcggcga ggggcgacac
      601 ccggcataca gccgatgcac ggacctctgt tacactgtca cgtttagcgg cgccgcgccc
      661 aagcgtcggc ttcaggccgc tcgcgcccct ggcgcgtcag ggaggcacct tgcaggacct
      721 cgtcatccgt ggggcacgcg ctcacaacct caagaacgtc gacctggtac tgccccgcaa
      781 caagttcatc gtcttcacgg gtgtgtcggg ctccggcaag tccaccctgg ccttcgacac
      841 catctacgcg gaagggcagc ggcgctacgt ggagagcctc tccgcctacg cgcggcagtt
      901 cctcggcatc atggacaagc cggacgtcga ctcgatcgac ggcttgagcc ccgccatcag
      961 catcgaccag aagaccacca gccataaccc gcgctcgacg gtcggcaccg tcaccgaggt
     1021 ccacgactac ttgcgcctcc tcttcgcgcg cgtcggcacc ccgcactgcc ccgtctgcgg
     1081 gcgcgtcatc caacggcagt cggccaccga gatagtcgac cgcctcatcg agcgcttctc
     1141 cggcaagcgc ggcatgctcc tggccccggt ggtgcgaggc cgcaagggcg agtaccggaa
     1201 gctcttcctc gacctgaaga aggagggctt cgcgcgcgtg cgcgtcgacg gcgtcgtgca
     1261 gaccatcgag caggcgctcg aggcggacct ggagcgtttc gagaaacacg acatcgacgt
     1321 ggtcatcgac cgcgtggtga tcgacgagaa cgaccgctcc cgcatcgcag agtcggcgga
     1381 gctggccctc acgaagggcg agggcctgct gcgcgccttc ttgcctgacg acaacgtcga
     1441 cgagctcttc agtgagaagt tcgcgtgccc ggaacacggc acgttcctgg aggagctgga
     1501 gccacgcacg ttctcgttca acgcgccgta cggcgcctgc agccattgca gcgggctcgg
     1561 ctacctccag cagttcgacc ccgagctgat catcccggac ccgacgctct ccgtcgccca
     1621 gggcgccatc gccccgtgga ctggcgcgcg cggc
//
LOCUS       DAOQYE010000543         1647 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_90748_length_1647_cov_3.575377, whole genome shotgun sequence.
ACCESSION   DAOQYE010000543 DAOQYE010000000
VERSION     DAOQYE010000543.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90748_length_1647_cov_3.575377"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="PKN52_08745"
     CDS             <1..272
                     /locus_tag="PKN52_08745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNR00077.1"
                     /translation="EGKATHTVLELLLEHESEEARAILARRASREGDVERMAELAIRS
                     GASEATRREIAAKVAEARAALEAFPASPERSALAELAGREGARVS"
     gene            355..>1647
                     /locus_tag="PKN52_08750"
     CDS             355..>1647
                     /locus_tag="PKN52_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HNR00078.1"
                     /translation="MSELDTDQVMRFFLDHPERPWHVQDIQRRFKVEDRAALRHLLAE
                     LADGGRLIRTRRRTYGLPQEMNMVLGRLQVASGGYGFVIPDAGGKDLYVPADRLGGAW
                     DTDRVMARPNPMKSDDGRPSGEVVRIIERGYQRVVGTLEYARGYAILRPDSVRLRERV
                     LLTPESVGKLAGGSRIVARMVWPEESGEREPFGEVVEFLGAEDDPEVETRAVIVKYDL
                     KAEFDQDTMKEAAAVPDEVTDEMMAGRTDCRDVTTVTIDGEDAKDYDDAISIERIGAG
                     KDGNLRVGIHIADVSYYVAEGTSLDKEAKERATSVYLPGRVLPMLPEELSNGICSLDE
                     AKARLALSLFVDVSRTGEVLSFEFKETVIRSDARLTYRQVQEFADGGRMPEGRRRLER
                     DLKVLLNLTQELRKARIGAGALDFNFTEARVEVDDQGAL"
BASE COUNT          267 a          543 c          608 g          229 t
ORIGIN      
        1 gtgaaggcaa ggcgacgcac acggtgctgg agctgctgct ggagcacgag tcggaggagg
       61 cccgcgccat cctggcgcgc cgcgcctccc gggagggcga cgtcgagcgc atggccgagc
      121 tggcgatccg gtcgggcgcc agcgaggcta cgcgacgtga gatagccgcg aaggtcgccg
      181 aggcgcgcgc cgcgctcgag gctttccctg cctcgcccga gcgctcggcg ctcgccgagc
      241 tggcgggacg agaaggggcg agggtcagct gaggtccccg tttggccggg cctgccgcgc
      301 gcatggcggg cggtggccgg ggcggcggac gttcacgcgc tagactgcaa cgatatgtca
      361 gagctagata ccgaccaggt gatgcgcttc ttcttggatc atccggagcg cccctggcac
      421 gttcaggaca tccaacggcg tttcaaggtc gaggaccgcg ccgccttgcg ccacctcctc
      481 gcggaactcg cggacggcgg ccgcctgatc cgcaccaggc gcaggacgta cggtctgcct
      541 caagagatga acatggtgct cggccgactc caggtggcgt cgggcgggta cggcttcgtc
      601 atccccgacg cgggcggcaa ggacctctac gtccctgccg acaggttggg cggcgcgtgg
      661 gacacggacc gcgtgatggc gcgcccgaac ccgatgaaga gcgacgacgg gcgcccgtcc
      721 ggcgaggtcg tccggatcat cgagcgcggc taccagcggg tcgtcggcac gctcgagtac
      781 gcgcgcggct acgccatcct gcgcccggac tccgtccggc tgcgggagcg cgtgctcctc
      841 acgccggagt ccgtcgggaa gctcgcgggc ggctcgcgca tcgtggcgcg catggtgtgg
      901 ccggaggagt cgggggagcg cgagcccttc ggcgaggtcg tcgagttcct cggtgcggag
      961 gacgacccgg aagtagagac ccgcgccgtc atcgtcaagt acgacctcaa ggctgagttc
     1021 gaccaggaca cgatgaagga ggccgccgcc gtccccgacg aggtgacgga cgagatgatg
     1081 gcggggcgca ccgactgtcg ggacgtcacc acggtgacga tcgacggcga agatgcgaaa
     1141 gattacgacg acgccatcag catcgagcgc atcggggcgg gcaaggacgg caacctgcgg
     1201 gtcggcatcc acatcgccga cgtcagctac tacgtggccg aggggacgag cctcgacaag
     1261 gaggcgaagg agcgcgccac gtccgtctac cttcctggtc gcgtgctccc gatgctgccg
     1321 gaggagctct ccaacggcat ctgctcgctc gacgaggcca aggcccgcct cgccctctcc
     1381 ctgttcgtcg acgtgtccag aacgggcgag gtgctgtcgt tcgagttcaa ggagacggtc
     1441 atccgctccg acgcgcgcct gacctatagg caggtgcagg agttcgccga cggcgggcgc
     1501 atgccggagg ggcgccgcag gttggagcgc gacctcaagg tcctgctcaa cctgacccag
     1561 gagctccgca aagctcgcat cggcgcgggg gcgctcgact tcaacttcac cgaggcgcgc
     1621 gtggaggtcg acgaccaggg cgccctg
//
LOCUS       DAOQYE010000544         1644 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_91037_length_1644_cov_3.576463, whole genome shotgun sequence.
ACCESSION   DAOQYE010000544 DAOQYE010000000
VERSION     DAOQYE010000544.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91037_length_1644_cov_3.576463"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            146..1639
                     /locus_tag="PKN52_08755"
     CDS             146..1639
                     /locus_tag="PKN52_08755"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNR00079.1"
                     /translation="MSAFPTGLAGTLAEVAESVRSESGSPGLGISVFTATDVILERGF
                     GHRDREAGLPVTPETVFGVASITKSFTALTALALQERGLLSIEDEVGRYLPFERLWSA
                     GAPARLHHFLSHSSGLPPTPTMTWLRHASQANDPVSAAPTLAETKLTLVPGVMGMPGT
                     PARSLTDVDAQAAAIPDDDAERRIAALASRVASFGGLSSWLDAHALPLAAPGELYSYS
                     NDAFCLSGGIIEQVAGVPYARAVEREVLTPLGMRRSRFGLEGVLADTDHATVYARDAG
                     GAVVRSPAWQTTGVMQAGGMLKSSLADLRVYVRHLMAGTGRVRELAEPRIASGPGATY
                     GLGLARRDDYHGLTLVSHGGSLKGVSSAIGYSPQLGVGFAILSNLEGVPAEQLFVKLV
                     NACAGLPISTVPYDPAPAAATPEELQPVLGDYLSGEPYGRTRLYLSDAGELRAEVGWP
                     PLDVPAFMAGQDEVALVYPARKVAARVLRDGSGKVWGLQSGRVMRRL"
BASE COUNT          210 a          586 c          596 g          252 t
ORIGIN      
        1 caggtcttca gcttcgtccg gcgggggtgc aacgttcggc gcgttggccg ggcgccggac
       61 gcgggttcgt gcggaccttc gcctggccgc acgtggaggg acgcggcgct agggcagcgc
      121 ggcgggcagt ggtgtaattg gcggcatgtc tgccttcccc accggcctcg ccggaacgtt
      181 ggcggaggtc gccgagtcgg ttcgttccga gtccggcagc cccggcctcg gtatcagcgt
      241 cttcaccgcc accgacgtca tcctcgagcg gggcttcggt caccgcgacc gcgaggccgg
      301 cctgccggtc acgcccgaga ccgtcttcgg agtcgcctcc atcaccaagt cgttcacggc
      361 gctcaccgcg ctggcgttgc aggagcgcgg tctgctgagc atcgaggacg aggtggggcg
      421 ctacctgccg ttcgagcgcc tctggtcggc cggcgcgccg gcgcgcctcc accacttcct
      481 gagccactcg agcggcctgc cgccgacgcc gaccatgacg tggttgcgcc acgccagcca
      541 ggcgaacgac ccggtctccg ccgcgccgac gctggccgag accaagctga cgctcgtgcc
      601 gggcgtcatg ggcatgcccg gaactcccgc caggtcgttg accgacgtcg acgcgcaagc
      661 cgccgccatc ccagacgacg acgctgagcg ccgcatcgcc gcgttggcct cacgggtcgc
      721 ctccttcggc ggcctgagct cgtggcttga cgcgcacgcg ctgcccctgg ccgcgccggg
      781 cgagctctac tcgtacagca acgacgcttt ctgcctgtcg ggcgggatca tcgagcaggt
      841 ggctggtgtg ccttacgcca gggcggtcga acgggaggtc ctgacacccc tcggcatgcg
      901 tcgctcgcgc ttcggcctcg aaggggtgct ggcggatacc gaccacgcca cggtctacgc
      961 gcgcgatgcc ggcggcgcgg tcgtgcgctc gccagcgtgg cagacgacgg gcgtcatgca
     1021 ggcgggcggc atgctcaaga gctccctcgc cgatctgcgc gtctacgtcc gccacctcat
     1081 ggcgggcacc gggagggtgc gcgaactggc cgagccgcgc atcgcgagcg gccccggcgc
     1141 tacctacggc ctaggcctgg cgcgccgcga cgactaccac gggctcacgc tcgtcagcca
     1201 cggcggcagc ctcaaaggcg tctcgagcgc catcggctat tcgccccagc tcggcgtcgg
     1261 gttcgcgatc ctgtcgaacc ttgagggggt gccggcggag cagctcttcg tgaagctcgt
     1321 caacgcctgc gcgggcttgc cgatcagcac ggtgccatac gacccggccc cggcggcagc
     1381 gacgccagag gagttgcaac ctgtcctggg cgactacctc tccggcgagc cgtacgggcg
     1441 gacacgcctc tacctatcgg acgcggggga gctgcgcgcc gaggtcggct ggccgccgct
     1501 cgacgtgccg gcgttcatgg ccggtcagga cgaggtggcg ctcgtctatc cggccaggaa
     1561 ggtggccgcg cgggtgctgc gcgacggctc cgggaaggtg tgggggttgc agtccgggcg
     1621 ggtgatgcgg aggctgtagc ctgt
//
LOCUS       DAOQYE010000545         1642 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_91239_length_1642_cov_2.413359, whole genome shotgun sequence.
ACCESSION   DAOQYE010000545 DAOQYE010000000
VERSION     DAOQYE010000545.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91239_length_1642_cov_2.413359"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(44..541)
                     /gene="dps"
                     /locus_tag="PKN52_08760"
                     /gene_synonym="pexB"
     CDS             complement(44..541)
                     /gene="dps"
                     /locus_tag="PKN52_08760"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: protein motif:HMM:NF006975.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HNR00080.1"
                     /translation="MPTATKTRLIDVKLDIAKADRERLVAILNQHLADTLDLQSQIKQ
                     AHWNVKGLGFQQVHLLFDDQAEVFEGFADMIAERVALLGGVAEGTVRMAAAASKLPEF
                     PTDEHAVPVFLALVRDRVAAYAKSTRQAIEAVSKIDEPTTEDLLTEVSRGTDQQLYFL
                     EAHLF"
     gene            complement(763..1530)
                     /locus_tag="PKN52_08765"
     CDS             complement(763..1530)
                     /locus_tag="PKN52_08765"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HNR00081.1"
                     /translation="MTIANDAARQNHPVTAFEVDESRLGEYALRRPAKVSGRVRLVAV
                     GHYDIVACGGTDMRSSAEALPIKLTGFERVRGGNTRITFRAGEEAVADYRAKHEAASA
                     LVRAFSAPVGELAGRGAQLQEELARSQYEAAGWRGRLARAYLERLAAQATGGVVSAVL
                     EGDDAALLPDLVEACQSLPSTVALLACVDDGQARLAFVAGPGAAIDVRPLLKEALVGL
                     GGRGGGRPDRAQGAAPASAAAVRDVLAQARLTLERPS"
BASE COUNT          228 a          574 c          594 g          246 t
ORIGIN      
        1 ctcgcgccgt cggcggtacg ccccggaccg tcggcggtac gcctcagaac agatgggcct
       61 cgaggaagta gagctgctgg tccgtgccgc gcgagacctc ggtgaggagg tcctcggtgg
      121 tcggctcgtc gatcttcgac acggcctcga tggcctggcg ggtgctcttg gcgtaggccg
      181 cgacgcggtc gcgcaccagc gccaagaaga cgggcacggc gtgttcgtcc gttgggaact
      241 cgggcagctt cgaggcggcg gccgccatgc gcacggtgcc ctccgccacg ccgccgagca
      301 gcgcgacgcg ctcggcgatc atgtcggcga agccctcgaa gacctcggcc tggtcgtcga
      361 agagcaggtg cacctgctgg aacccaaggc ccttcacgtt ccaatgggcc tgcttgatct
      421 ggctctggag atcgagcgtg tcggccaggt gctggttgag gatggccacg aggcgctctc
      481 ggtccgcttt cgcgatgtcg agcttgacgt cgatgagtct ggtcttcgtt gcggtaggca
      541 tacgaaatct cctccgagcc tcagattacc ggttgggagg cgtagcggcc gtgtgcgcgg
      601 gcacgtctgg tacgtgagca tgagggtgcc gcccgcccgg ccgcgcggaa ggacggcgtg
      661 cgcgcccgtg tccggatgaa tgcgcgcgcg gcccgtccgg accagttggg ggcgacccgc
      721 ctgataagcg ggcgcgaccc cgcctataca ggccggggcg gtctagctcg gtcgctccaa
      781 tgtcaggcgc gcctgcgcga gcacgtcgcg cacggccgcg gcggatgccg gagcggcccc
      841 ctgggccctg tccggccgtc cgccgccccg tccgcccagc cccaccaagg cttccttgag
      901 cagcggtctc acatcgatgg cagcgcctgg gccggcgacg aaggcgagcc gcgcctggcc
      961 gtcatcgacg caggccagta gagcgaccgt gctgggcagg gactggcagg cctcgacgag
     1021 atccggcaac agcgcggcgt cgtcgccttc gagcacggcg ctcaccacgc cgccggtggc
     1081 ctgggccgcg agccgctcga ggtaggcgcg agcaaggcgg ccacgccaac ccgcggcttc
     1141 gtactggcta cgggccagct cttcctgcaa ctgcgctccg cgcccggcca actcccccac
     1201 gggcgcgctg aacgcgcgca cgagggccga ggcggcctcg tgcttggcgc ggtagtcggc
     1261 taccgcttcc tcgccggcgc ggaacgtgat acgcgtgttg ccgccgcgca cgcgctcgaa
     1321 gcccgtgagc ttgatgggta gcgcctcggc cgacgagcgc atgtccgttc cgccgcaggc
     1381 cacgatgtcg taatggccga cggccaccag ccgcacgcgc ccggagacct tggcgggccg
     1441 ccggagcgcg tactcgccca ggcgcgactc gtctacctcg aaggccgtga cggggtggtt
     1501 ctgcctggcg gcgtcgttcg cgatggtcac gtccaaggcc ggcagttcgc cgccgtaccg
     1561 ctacgccgcc acgcaggcca cgatggatgt gaacggggcc tggacacagg tcggtggcgg
     1621 cgcggcgtac aacaacgtct tc
//
LOCUS       DAOQYE010000546         1641 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_91357_length_1641_cov_2.261034, whole genome shotgun sequence.
ACCESSION   DAOQYE010000546 DAOQYE010000000
VERSION     DAOQYE010000546.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91357_length_1641_cov_2.261034"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1183
                     /locus_tag="PKN52_08770"
     CDS             <1..1183
                     /locus_tag="PKN52_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNR00082.1"
                     /translation="GQELELSRAAAARYMALHPDVTVNVLETPDLTDNRQALYLQMFE
                     AQSSDVDVFQIDVIWPGDMAEHFVDLYEYGAKDVADEHFPAIIENNTVDGRLVGIPWF
                     TDAGLLYYRTDLLEKYGFDGPPATWDELTSMAQTIQDGERAAGNQDFWGFVFQGNSYE
                     GLTCDALEWVSSYGGGSIVSPDGTITINNAKAAAALDKAASWVGTIAPNGVTSFDEES
                     ARNMFQAGNAAFMRNWPYAYSLGNASDSVIAGKFDVSPLPAGPDGSPAATLGGWQLAV
                     SKYSKNPAVAADVALFLASYDEQKIRAVEGSYNPTIKALYQDEDVLAAVPFFGSLYDV
                     FINAVARPSTATAPDYAAVSRAFYTGVHSVMTGQATGAEAVAEIEADIEDVTGFPTGQ
                     P"
     gene            1392..>1641
                     /locus_tag="PKN52_08775"
     CDS             1392..>1641
                     /locus_tag="PKN52_08775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00083.1"
                     /translation="MTGDSRAGGRQSRDRDRSSDRSSDRSRRGTASLLRAERRTAYGL
                     LLPTFIILAVIAAYPFGRVVYSSLTNAKFASSQPTSFIG"
BASE COUNT          276 a          617 c          522 g          226 t
ORIGIN      
        1 cggccaggaa ctcgagttga gcagggccgc tgcggcgcgc tacatggcac tccacccgga
       61 cgtgaccgtc aacgtcctcg agacccccga cctgacggac aaccgccagg cgctctacct
      121 gcagatgttc gaggcgcagt cctccgacgt agacgtgttc cagatcgacg tcatctggcc
      181 gggcgacatg gccgagcact tcgtcgacct ctacgagtac ggcgccaagg acgtggcaga
      241 cgagcacttc ccggccatca tcgagaacaa caccgtggac ggccgcctcg tcggcatccc
      301 gtggttcacg gacgccggcc tcctctacta ccgcaccgac ctgctggaga agtacggctt
      361 cgacgggccg cccgccacct gggacgagct caccagcatg gcccagacga tccaggacgg
      421 cgagcgcgcc gccggcaacc aggacttctg gggcttcgtc ttccagggca actcttacga
      481 gggcctcacg tgtgacgccc tcgagtgggt ctcctcttac ggcggcggct cgatcgtcag
      541 ccccgacggg accatcacga tcaacaacgc caaggccgcc gcggccctcg acaaggcggc
      601 gagctgggtc ggcaccatag ccccgaacgg cgtgacgagc ttcgacgagg agtcggcccg
      661 caacatgttc caggccggca acgcggcctt catgcgcaac tggccgtacg cctactcgct
      721 cggcaacgcc tccgacagcg tcatcgccgg caagttcgac gtcagcccgc taccggccgg
      781 cccggacggc tcccccgccg cgaccctcgg cggctggcag ctcgcggtct ccaagtacag
      841 caagaacccg gccgtggccg ccgatgtcgc cctcttcctc gcctcgtacg acgagcagaa
      901 gatccgcgcc gtcgaaggca gctacaaccc gaccatcaag gccctgtacc aggacgagga
      961 cgtcctcgcc gccgtgccgt tcttcggctc gctctacgac gtcttcatca acgctgtcgc
     1021 caggccctcc accgccaccg ccccggacta cgcggccgtc tcgcgcgcct tctacacggg
     1081 ggtgcatagc gtgatgacgg gtcaggccac cggcgccgag gccgtcgccg agatcgaggc
     1141 cgacatcgag gacgtgacgg gcttccccac cgggcagccc tgagccccgg gtagggcgga
     1201 ccggcaccgt acccggtccc ttcggcgcga cccccaggtt cgtgcgcgcc gaggtggcgg
     1261 gatcccggtc acttggaagc ggggccgacc ttcatccggt cggccccatc cacacctgcg
     1321 ggtcgccgtt cgcccgtaga cgcaggcggc ggggaccggc tcgcgaggtg actaaggagg
     1381 agagctagtg catgaccggt gacagccggg cgggcgggcg ccagagccga gaccgcgacc
     1441 gcagcagcga ccgcagcagc gaccgcagcc gccgcggcac ggcgagcctg ctgcgggcgg
     1501 agcggcggac ggcctacggc ctcctgctcc ccaccttcat catcctggcg gtcatagccg
     1561 cctacccgtt cgggcgggtc gtgtactcga gcctgaccaa cgccaagttc gccagctcgc
     1621 aacccacgag cttcataggg c
//
LOCUS       DAOQYE010000547         1640 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_91406_length_1640_cov_3.390536, whole genome shotgun sequence.
ACCESSION   DAOQYE010000547 DAOQYE010000000
VERSION     DAOQYE010000547.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91406_length_1640_cov_3.390536"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1640
                     /locus_tag="PKN52_08780"
     CDS             <1..>1640
                     /locus_tag="PKN52_08780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00084.1"
                     /translation="RSNQIAIRDRVSALEASDAAQNAAIADLQGRVAALEENPLGISG
                     SISVTYFVGRVIGQEFDVDRAYGLNNLRNMGASVFSSGAAELDGDRRGTRGYRTEVGE
                     VAQDRHDIEHTPGSATAILDLSFATKFGFDGEGSPNKLNSFSGVLSIAMDTQRVTGPI
                     VSGSEPLLNWFEIRNFMTTFEPIGAVPLTFAFGTNVETSFTPYVVGTDDPGFVATIGA
                     PSFLAFLNPGITAVYVSRNDATDVNYNQEWYGNYLRGARLTVSPLEGLTLGGSFAQYA
                     ERAGDKDDYLADNQQLTVWGVDGSLALSIFDLGFEWANGSGGATAESVLYATLDVDTA
                     GIPILSSLGANYRNISDSWTANGYDLGADADAFPFVEDQSGFGVNAGLSLFIVDLSAY
                     FDSYGTVAGDSAMSFGVDASANLFAGFSLGGWYHSASVNGTTVDDLRTFVDASEAAVA
                     LAGTDIERDNNYNTGFGVTLKHDGAAANALIKGLNISAGYSQTEADFSKTTLFVDADY
                     ALNVSILSLTPYVGYRMVNDADAGTDDTTTLRVGTGLS"
BASE COUNT          286 a          600 c          475 g          279 t
ORIGIN      
        1 tccgcagcaa ccagatcgcc attcgcgacc gcgtctcggc cctggaagct tcggacgccg
       61 cccagaacgc cgccatcgcg gacctgcagg gccgcgtcgc cgcgctcgaa gagaacccgc
      121 tcgggatctc cggcagcatc tcggtcacgt acttcgtcgg ccgtgtcatc ggccaggagt
      181 tcgatgtcga ccgcgcttac ggcctgaaca acctgcgcaa catgggcgcg tcggtgttct
      241 cgtccggagc ggccgaactc gatggtgacc gtaggggcac cagaggctac cgcacggaag
      301 tcggcgaagt cgctcaggat cgtcacgaca tcgagcacac ccccggctcc gccaccgcca
      361 tcctcgacct gagcttcgcc acgaagttcg gcttcgatgg cgaggggagc ccgaacaagc
      421 tcaacagctt cagcggcgtg ctttcgatcg ccatggatac ccagcgcgtc acgggcccca
      481 tcgtctccgg ctccgaaccc ctgctcaact ggttcgagat ccgcaacttc atgacgacgt
      541 tcgaacccat cggcgccgtg ccgcttacgt tcgcgttcgg cacgaacgtc gagacgagct
      601 tcactccgta cgtcgtcggt accgatgacc ctggcttcgt cgccaccatt ggcgctccca
      661 gcttcctcgc cttcctcaac cccgggatca cggccgtcta cgtcagcagg aacgacgcta
      721 cggacgtgaa ctacaaccag gagtggtacg gcaactacct gcgtggcgcc cgactgacgg
      781 tcagccccct cgagggcctg acgctcggtg gcagcttcgc gcagtacgcc gaacgcgccg
      841 gcgacaagga cgactacctc gccgataatc agcagctcac ggtctggggc gtcgacggta
      901 gcctcgcgct gtccatattc gacctcggct tcgagtgggc gaacggctcc ggcggcgcga
      961 ccgccgagtc ggtcctctat gccacgctcg acgttgatac cgcgggtatc cccatcctct
     1021 cgagcctcgg cgccaactac cgcaacatca gtgacagctg gactgccaac ggctacgatc
     1081 tcggcgctga cgccgacgcc ttcccgttcg tggaagacca gagcggcttc ggcgtgaacg
     1141 ctggcctcag cctgttcatc gtcgacctga gcgcgtactt cgactcctac ggcacggtcg
     1201 ccggtgacag cgccatgtcc ttcggcgtcg acgcctcggc caacttgttc gcgggcttca
     1261 gcctcggcgg ctggtaccac tcggcctccg tcaacggtac gaccgtcgac gacctccgca
     1321 cgttcgtcga tgcgagcgag gccgctgtgg cccttgccgg caccgacatc gagcgcgaca
     1381 acaactacaa caccggcttc ggcgtcacgc tcaagcacga cggcgccgct gccaacgccc
     1441 tcatcaaggg cctgaacatc tcggcgggct actcgcagac cgaggcggac ttcagcaaga
     1501 cgaccctgtt cgtcgatgcc gactacgcgc tcaacgtctc gatcctctcg ctgaccccgt
     1561 acgtcggcta ccgcatggtc aacgacgccg atgccggcac ggatgacacc accacgctcc
     1621 gcgttggtac gggtctctcc
//
LOCUS       DAOQYE010000548         1639 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_91512_length_1639_cov_2.976641, whole genome shotgun sequence.
ACCESSION   DAOQYE010000548 DAOQYE010000000
VERSION     DAOQYE010000548.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91512_length_1639_cov_2.976641"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /locus_tag="PKN52_08785"
     CDS             complement(<1..598)
                     /locus_tag="PKN52_08785"
                     /inference="COORDINATES: protein motif:HMM:NF040248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNR00085.1"
                     /translation="MNLTTFIVKRVLAAVPLLLGISVILFGVLHLAPGGPLDVYADIP
                     GVSHEALENIKRELGLDQPLPVQYVKWLGAAARGQWGYSIRTGRPVAKDIRERMPATF
                     LLGGTAFLLALAAALPLGILSAVRQYSVLDYTFTFLSFIGFSMPIFWLALMLQSLFAV
                     HWQLLPSAGMYTIGDGGLADRLKHLALPASILSVAYMAS"
     gene            complement(663..>1639)
                     /locus_tag="PKN52_08790"
     CDS             complement(663..>1639)
                     /locus_tag="PKN52_08790"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNR00086.1"
                     /translation="VIVERNPYYFETDANGVQLPYLDRIVFKIIPDSNTQTTQLRSGE
                     VGFAYNVPYTSLATLRNTPNLEIISAKTLAFRHVTFNLRNELLADLAVRQAFAHAIDR
                     TTLNRALGDYMLPIDTFVVSTFAYASDDVPTYGYDPELARQLLLDAGYAPGRDGVMEK
                     DGKRLSFDFMTQAGRAEYETAQQVLIAQLKAIGIELKPDNKAGAALSEARRNGGFDVW
                     YSGWITPADPIDSYVSFYATNGFNNGGAYSNPAADAALYAAAGSLDPEEVEARMHEAQ
                     QIVLSDLPSIPLFETPSIVVTTNKLQGFVSNPTNQTNFRQVAPWWLAK"
BASE COUNT          275 a          448 c          628 g          288 t
ORIGIN      
        1 aactcgccat gtacgcgacc gagaggatgg aagccggcag cgcgaggtgc ttgagcctgt
       61 cggcgaggcc gccgtctccg atcgtgtaca tcccggcgga gggcagcaac tgccagtgaa
      121 cggcgaacaa cgattgcagc atgagggcga gccagaagat cggcatggag aaaccgatga
      181 aagataggaa cgtgaaggtg tagtcgagaa cgctgtactg ccgtacggcg ctgagtatgc
      241 cgagggggag cgcggccgcg agggccagaa ggaacgccgt gccgccgagc aggaacgtgg
      301 ccggcatgcg ttcgcggatg tccttcgcca cgggccggcc cgtacggatg ctgtaacccc
      361 actgaccgcg ggcggccgcc cccagccact tgacgtactg gacgggcagc ggctggtcga
      421 ggcccagctc gcgcttgatg ttctcaagcg cctcatggct gacgccgggg atgtcggcgt
      481 atacgtcgag tgggccgccc ggcgccagat gcagtactcc gaagaggatg acggagatgc
      541 cgaggagcag cgggacggca gcaaggaccc gtttgacgat gaaggttgtg aggttcacgg
      601 tcgtccttgc ctgagagcgg ctgggagggg cgcatgcgcc cctcccagcg gggtggccgt
      661 tcctacttgg ctagccacca gggcgcgact tgccggaagt tggtctggtt ggtcgggttg
      721 gatacgaacc cctgcagctt gttggtcgtc acgacgatgg acggggtctc gaagaggggg
      781 atggagggca ggtcggagag cacgatctgc tgggcctcgt gcatgcgggc ttcgacctct
      841 tccgggtcga gcgaacctgc cgcggcgtag agggcggcgt cggcggccgg gttgctgtac
      901 gcgccgccgt tgttgaagcc gttggtggcg tagaaggaga cgtaggagtc gatcgggtcg
      961 gcaggcgtga tccagccgga gtaccagacg tcgaacccgc cgttgcgccg ggcttccgag
     1021 agggcggcgc cggccttgtt gtctggcttc agctcgatgc cgatggcctt cagctgggct
     1081 atcaggacct gttgcgccgt ctcgtactcg gcgcggcctg cctgcgtcat gaagtcgaac
     1141 gagagtcgct tgccgtcttt ctccatgacg ccgtcgcggc cgggcgcgta gcccgcgtcg
     1201 aggagcagct ggcgcgcgag ttcggggtcg tagccatagg tgggcacgtc gtcgctggcg
     1261 tacgcgaagg tggagaccac gaacgtgtcg atggggagca tgtagtcgcc gagcgcgcgg
     1321 ttcagcgtgg tgcgatcgat ggcgtgcgcg aacgcctgcc ggacggcgag gtcggcgagt
     1381 agctcgttcc tgaggttgaa cgtgacgtga cggaaggcca gggtcttggc gctgatgatc
     1441 tccaggttgg gcgtgttcct gagcgtcgcc aacgaggtgt aggggacgtt gtaggcgaaa
     1501 ccgacctcgc cggagcggag ctgcgtcgtc tgagtgttcg agtcggggat gatcttgaag
     1561 acgatgcggt ctaggtacgg cagctgaacg ccgttggcgt cggtctcgaa gtagtagggg
     1621 ttgcgctcga cgatgacgt
//
LOCUS       DAOQYE010000549         1639 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_91518_length_1639_cov_2.848485, whole genome shotgun sequence.
ACCESSION   DAOQYE010000549 DAOQYE010000000
VERSION     DAOQYE010000549.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91518_length_1639_cov_2.848485"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            464..1405
                     /gene="argF"
                     /locus_tag="PKN52_08795"
     CDS             464..1405
                     /gene="argF"
                     /locus_tag="PKN52_08795"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HNR00087.1"
                     /translation="MEPTRLPSLAGKDFLSLADLTKEEFAGLIARAIRLKTDWKRGVR
                     PAPMQHKTLAMIFEKQSLRTRTTFDVAMAQLGGHSVLLSQDYIGMGVRETMRDVANNL
                     SRWVDGIMLRTHGHERLLELAEHAAVPVINGLSDYLHPCQLVADYIALTETFGKLNGV
                     KLTYVGDGNNVANSHVNAAALAGVHLTIACPPGYEPDGGVLATARARGGSISISHDPR
                     EAVKGADALYTDVWVSMGMEEDTARRKRDFAGYTITPEWFADLAPRGVFMHDLPAHYG
                     EECTEECVYHERSIVFDQAENRLHAHKAVIFQCLAER"
     gene            1421..>1639
                     /locus_tag="PKN52_08800"
     CDS             1421..>1639
                     /locus_tag="PKN52_08800"
                     /inference="COORDINATES: protein motif:HMM:NF013298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00088.1"
                     /translation="MADGLIRAGVLGASGYGGAGLIERLERHPGVALVAIGSRAYLGK
                     PLAASWPHLTGLGNDLRFSSDGGESWRRQ"
BASE COUNT          252 a          579 c          557 g          251 t
ORIGIN      
        1 tgcgtgctgt tcgccgtcgt gttgggcgca ctgttcggcg tggtcggcag gctggccggc
       61 ggcaagcgcg tgatcccgtt cgggccctac ctgctcgcgg gcggcctcac cgcgctcctc
      121 ttcggcgacg cgactatccg ctggtacctc ggcctgctcg gcgtcggctg accgaccgcg
      181 cggccgtgcc aagtcgcgcg cgaccgcgcg ccgttcccgt gccgttcccg cgcgccggtc
      241 ccgcgctcac ggttgaacac gcagtaacgg gtcgggcccg gccggcaagg gagcgtgcgc
      301 atcccggcag tcctaccagg cgacatccga tgctatatgg tgttccgcgt tggacgcgcc
      361 ccacgcgttc agcgcgcctc acgcgtgaac gcgcctcgtt gatgagggcg ccccgctgcg
      421 tgaatgcgcc tctactcgct tgaggcggcg cgattggagt ggaatggagc caacccgtct
      481 tccgagcttg gcaggcaagg acttcttgtc gctcgccgac ctgaccaagg aggagttcgc
      541 cggcctgatc gccagagcca tccggctcaa gaccgactgg aagcgcggcg tgcgaccggc
      601 gccgatgcag cacaagacgt tggccatgat cttcgagaag cagtcgctca ggacgcggac
      661 caccttcgac gtcgcgatgg cgcaactcgg tggccactcc gtcctgctga gccaggacta
      721 catcggcatg ggggtgcgcg agaccatgcg ggacgtcgcg aacaacctca gccgttgggt
      781 cgacggcatc atgcttcgca cccatgggca cgagcggctc ctcgaactcg ccgagcacgc
      841 cgccgtgccg gtcatcaacg gcctctccga ctacctgcac ccgtgtcagc tcgtggccga
      901 ctacatcgcg ctgaccgaga cgttcggcaa gctgaacggc gtgaagctca cgtacgtcgg
      961 cgacggcaac aacgtcgcca actcgcacgt gaacgccgcc gcgctcgccg gcgtgcacct
     1021 gaccatcgcc tgcccgcccg gctacgagcc cgacggcggg gtgctcgcga cagccagggc
     1081 gcgcggaggc tccatcagca tctcccacga tccgcgcgag gccgtcaagg gcgcggacgc
     1141 gctctacacg gacgtctggg tctccatggg catggaggag gacacggcgc gccgcaagcg
     1201 cgacttcgcc ggctacacca tcacgccgga gtggttcgcc gacctcgccc cccgcggcgt
     1261 cttcatgcac gacctgccgg cgcattacgg cgaagagtgc acggaagagt gcgtctacca
     1321 cgagcgcagc atcgtcttcg accaggcgga gaaccgcctg cacgcccaca aggccgtgat
     1381 cttccagtgc ttggcggagc gctgaggtaa ggaggcaggc atggctgacg gactgatcag
     1441 ggcgggcgtg ctgggcgcca gcggttatgg aggcgccggc ctcatcgagc gcctcgagcg
     1501 ccatccgggc gtggcgcttg tcgccatcgg ctcccgggct tatctcggca agcccctggc
     1561 ggcgagctgg ccgcacctga cgggcctggg caacgacctg cgcttctcga gcgacggcgg
     1621 cgagagctgg cggcgccag
//
LOCUS       DAOQYE010000550         1637 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_91719_length_1637_cov_2.555626, whole genome shotgun sequence.
ACCESSION   DAOQYE010000550 DAOQYE010000000
VERSION     DAOQYE010000550.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91719_length_1637_cov_2.555626"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="PKN52_08805"
     CDS             complement(<1..225)
                     /locus_tag="PKN52_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00089.1"
                     /translation="MSKDDRTTTGARAAHLADSHDLIRVQGARENNLKDVSVEIPKRR
                     LTVFTGVSGSGKSSLVFGTIAAESQRMINET"
     gene            complement(319..729)
                     /locus_tag="PKN52_08810"
     CDS             complement(319..729)
                     /locus_tag="PKN52_08810"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNR00090.1"
                     /translation="MNITIHQAMLPHEDPAASLAFYKDALGFEVRNDVEYAGMHWITL
                     GAPDQQDMAIVLYPPAAAPGLTDDERRMIAEMMAKGTYASINLATRDLDAAFEHIAAS
                     GAEVVQEPTDQPYGIRDCAVRDPAGNLIRIQELR"
     gene            complement(740..1183)
                     /locus_tag="PKN52_08815"
     CDS             complement(740..1183)
                     /locus_tag="PKN52_08815"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNR00091.1"
                     /translation="MSAGSPAGARLAELARLRRVRDRMDREYMLPLNVEALASEVGMS
                     AGHLSRQFRLAYGESPYSYLMTRRIERAMALLRQGQLNVTEVCFEVGCSSLGTFSTRF
                     TELVGMPPSVYRREAAGAMNGMAPCVAKQVTRPIRNREAPSEGDS"
     gene            1314..>1637
                     /locus_tag="PKN52_08820"
     CDS             1314..>1637
                     /locus_tag="PKN52_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HNR00092.1"
                     /translation="MSLVDILVWVGTITFAASGALVAVRRRFDLIGVIVLASVTAIGG
                     GSIRDVMVGALPPASLSNEPLLWAVGATALLVLFVPHRIREESRLLYSLDTFGLALFA
                     ALGAER"
BASE COUNT          212 a          574 c          561 g          290 t
ORIGIN      
        1 cgtctcgttg atcatccgct gcgactcggc ggcgatggtg ccgaacacga gcgagctctt
       61 gcctgagccc gagacgccgg tgaacacggt caaccggcgc ttcgggatct cgacgctcac
      121 gtccttgagg ttgttctcgc gcgcgccctg cacgcggatg aggtcatggc tatccgcaag
      181 gtgcgccgca cgcgcgcccg tggtcgtcct gtcgtccttg ctcactcgcg tctccgttct
      241 cgcacaagca ccaactcggc cccccggcgc caccaggtcg gctagcgcgg aaggccgagc
      301 ttacggcaac tacgggctct agcgcagttc ctggatgcgg atgaggttac cagccgggtc
      361 gcgcaccgcg cagtcgcgga tgccgtacgg ctggtcggtg ggctcctgca ctacctcggc
      421 gccgctggcg gcgatgtgct cgaaggccgc gtcaaggtcg cgggtggcca ggttgatgct
      481 ggcgtaggtg cccttcgcca tcatctccgc gatcatgcgg cgctcgtcgt cggtgagacc
      541 gggcgcggcg gcgggcgggt agagcacgat cgccatgtcc tgctggtcgg gcgcgcctag
      601 cgtgatccag tgcatgcctg cgtactcgac gtcgttgcgg acctcgaagc cgagggcgtc
      661 cttgtagaag gcgagagagg cggcggggtc ttcgtgcgga agcatggctt ggtgaatggt
      721 gatgttcatg ccgcccatgt taggagtcgc cctcgctcgg cgcttctcga ttcctgatcg
      781 gccgcgtcac ctgcttggcg acgcacggcg ccatgccgtt catcgctccc gccgcctcgc
      841 ggcggtacac gctcggcggc ataccgacga gctcggtgaa gcgcgtgctg aaggtaccga
      901 ggctggagca gccgacctcg aagcagactt ccgttacgtt gagctgcccc tggcgcagca
      961 aggccatggc gcgctcgatg cggcgcgtca tgaggtagct gtacggcgac tcgccgtacg
     1021 cgaggcggaa ctgcctgctc aggtggccgg ccgacatgcc gacctcgctg gcgagcgcct
     1081 cgacgttcag cggcagcatg tactcgcggt ccatgcggtc gcgaaccctg cgcagccgcg
     1141 ccagctccgc gaggcgcgct cccgctgggc tgccggcgct catgcgcgac cgccgataag
     1201 cgaagcaccg cccgtcatgg ggcgatgatg ccatgcggcg gcgccctgct ccccgacgcg
     1261 ctcgacaccg cgcgcccctc gccgcgcgtg taagccgctt gtatgctgcc gccatgagct
     1321 tggtcgacat cctcgtctgg gtcggcacca tcacgttcgc ggcgagcggc gcgctcgtcg
     1381 ccgtgcgcag gcgcttcgac ctcatcggcg tgatcgtgct cgcctccgtg accgccatcg
     1441 gtggcggctc catccgcgac gtgatggtgg gcgcgctgcc tccggcctcg ctctcgaacg
     1501 agcccctcct atgggccgtg ggggccacgg cgctgctcgt gctgttcgtg ccgcaccgca
     1561 tccgggagga gagccgcctc ctctactcgc tcgacacgtt cggcctcgcc ctcttcgcgg
     1621 cgctcggggc cgaacgc
//
LOCUS       DAOQYE010000551         1637 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_91738_length_1637_cov_2.128319, whole genome shotgun sequence.
ACCESSION   DAOQYE010000551 DAOQYE010000000
VERSION     DAOQYE010000551.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91738_length_1637_cov_2.128319"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(149..1138)
                     /locus_tag="PKN52_08825"
     CDS             complement(149..1138)
                     /locus_tag="PKN52_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNR00093.1"
                     /translation="MHWFIIKRLLLLPVLLLIFSIVSFALIQAPPGDFLTSYVAELAA
                     GGTSIDQVEIEALRTRYGLDQPIYVQYTRWVGNLLRGDLGVSLEWRRPNSELIGERLV
                     LTLILTTFSLLFTWAVAIPIGIFSATRQYSLLDYVFTILNYVGLATPNFMLALVLMWV
                     AFDKFGVTVTGLFSPQYIDAAWSWARVGDLMSHIWLPMIVLGVAGTAQIARVVRANLL
                     DELSKPYTEMARAKGLPEWRLVIKYPTRVAIAPVVSTLGWYFPQLLSGGLVVSVVMSL
                     PTIGPLLLRALVGQDMYLAGTIILIFCMLAVLGALVSDILLVVLDPRIKLESA"
     gene            complement(1238..>1637)
                     /locus_tag="PKN52_08830"
     CDS             complement(1238..>1637)
                     /locus_tag="PKN52_08830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00094.1"
                     /translation="WNEDTTAFPFTGNPKIDPRSDPATIFAVESRRWYATDGAQGKEP
                     APGIARIVDIIEEAKTVGVEKQIELAQELFRHAVDELYGFGLVGLTPMVQGVVVVNDN
                     LMNVPAVVANDWPLRTPGDSRPEQFFYKTN"
BASE COUNT          290 a          483 c          615 g          249 t
ORIGIN      
        1 gaggcacacg acgccgagct tgtgcttcgt gaagcgcagc cacgtgagct tccacgccgg
       61 ggtgacatag acggagggct ccttggcccg ctccgcggcc ggcaggacaa cgtcgctcat
      121 gcgctctcca gcttcagggc ggcgcctctc atgcgctctc cagcttgatg cgcgggtcga
      181 ggaccacgag gaggatgtcg gagacgagcg cgccgaggac ggcgagcatg cagaagatga
      241 ggatgatggt gccggcgagg tacatgtcct ggccgacgag cgcgcgcagg agcagcgggc
      301 cgatggtcgg caggctcatc acgacggaga ccacgaggcc gcccgacagg agctgcggga
      361 agtaccagcc gagggtgctg acgaccggcg cgatcgcgac gcgcgtgggg tacttgatga
      421 cgaggcgcca ctcgggcagg cccttggccc gcgccatctc cgtgtacggc ttgctcagct
      481 cgtcgaggag gttggcgcgc acgacgcggg cgatctgggc cgtgccggca acgccgagga
      541 cgatcatcgg cagccagatg tggctcataa ggtcgccgac gcgcgcccac gaccacgccg
      601 cgtcgatgta ctggggcgag aagaggcccg tgaccgtgac cccgaacttg tcgaacgcca
      661 cccacatgag cacgagggcg agcatgaagt tgggcgtggc caggccgacg tagttgagga
      721 tcgtgaacac gtagtcgagc agcgaatatt ggcgtgttgc ggagaagata cctatgggga
      781 tggcgacggc ccacgtgaac aggagcgaga aggtcgtcag gatgagggtg agcacgaggc
      841 gctcaccgat gagctcgctg ttcgggcgcc gccactcgag ggagactccg aggtcgcccc
      901 gcagcaggtt gccgacccag cgcgtgtact ggacgtagat gggctggtcg aggccgtagc
      961 gggtccggag cgcctcgatc tcgacctggt cgatggaggt gccgcccgcc gccagctcgg
     1021 cgacgtagct agtgaggaag tcgcctggcg gggcctggat gagcgcgaaa gagacgatcg
     1081 agaagatcag gagcaggacc gggagcagca ggagtcgctt gatgatgaac caatgcaaga
     1141 gacgctctcc taaagcggtt cgtgctcgtt actgcggcgg tgccttgggc cgttcgggcg
     1201 cccctcgcta aggagcgccc gaacgggatg aacgaggtca gttcgtcttg tagaagaact
     1261 gctcggggcg ggagtcgccc ggggtgcgga gcggccagtc gttcgcgacg accgcgggga
     1321 cgttcatgag gttgtcgttg acgacgacga cgccctggac catcggggtc aggccgacga
     1381 ggccgaagcc gtacagctcg tccaccgcgt ggcggaagag ctcctgggcc agctcgatct
     1441 gcttctcgac gccgaccgtc ttggcctctt cgatgatgtc gacgatgcgg gcgatgccgg
     1501 gcgccggctc cttgccctgc gcgccgtcgg tggcgtacca gcgccgcgac tcgaccgcga
     1561 agatggtcgc ggggtcggag cgggggtcga tcttggggtt gcccgtgaag gggaacgccg
     1621 tggtgtcctc gttccag
//
LOCUS       DAOQYE010000552         1636 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_91777_length_1636_cov_3.254269, whole genome shotgun sequence.
ACCESSION   DAOQYE010000552 DAOQYE010000000
VERSION     DAOQYE010000552.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91777_length_1636_cov_3.254269"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            158..748
                     /locus_tag="PKN52_08835"
     CDS             158..748
                     /locus_tag="PKN52_08835"
                     /inference="COORDINATES: protein motif:HMM:NF016471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HNR00095.1"
                     /translation="MEIREGQAIELRDIESKDGAEIGRFAGFSTSYDQPYDIYGGPDR
                     GGFTEIMGVGAWTRTINERVDVNLLENHEGRPFARVGNGTLELSEPSDLGVMNLAALD
                     LRRGDARDLYLTLERGDYCKMSCAFMVLRDKWNPGYTQRTILEVVGFDSSVVTFPANP
                     NTLATVGDGTPTSPKARGASGVGVELARAEWYRLAG"
     gene            871..1053
                     /locus_tag="PKN52_08840"
     CDS             871..1053
                     /locus_tag="PKN52_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00096.1"
                     /translation="MFELPPTDDPKERARWLLGVLLLAERHEACEFLSLGGRRPFWPN
                     HQDEGCPYELVERWDG"
     gene            1046..1210
                     /locus_tag="PKN52_08845"
     CDS             1046..1210
                     /locus_tag="PKN52_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00097.1"
                     /translation="MAKQSPHKRWKGCAMCKWYKFKDNGQAQRQPLAVLRLAGRDRRL
                     KRHEIHQDGW"
     gene            1384..>1636
                     /locus_tag="PKN52_08850"
     CDS             1384..>1636
                     /locus_tag="PKN52_08850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00098.1"
                     /translation="MDFLDLLRERLSGMLAERSKIDEQKLNLDEKFEGLMAEARRDGR
                     NELTDDEAATIEAMKTERKALDERRAKLDQDIADAEQREA"
BASE COUNT          294 a          487 c          578 g          277 t
ORIGIN      
        1 ccggcggcgc cggtggtgac gaatggtggt gggaacgatg ggcagccgtc tgcgactccc
       61 tgacgaggtg gtggcccggc tccaagaggc tggtgtcgac cttggcgtgc gttccatgcc
      121 tgaccgtcgc aggtcgcagg tcggtcggtt cgctgaggtg gagatccgtg agggtcaggc
      181 aatcgagctg cgtgacatcg agtcgaagga cggcgctgag atcggccggt tcgctgggtt
      241 ctcaacctcc tatgaccagc cttatgacat ctacggcggc cccgaccgag gtgggttcac
      301 tgagatcatg ggcgtcggtg cgtggacccg gacgatcaac gagcgggtcg acgtcaacct
      361 gctcgagaac cacgaggggc gcccgttcgc aagggtgggc aacggcaccc tggagttgtc
      421 ggagccgagc gatctcgggg tcatgaacct cgccgcgctc gacctccgcc gtggtgacgc
      481 tcgcgatctg tacctgacgc tggaacgtgg cgactactgc aagatgtctt gcgcgttcat
      541 ggtgttgcgg gacaagtgga accccggtta cacgcaacgc acgatcctgg aggtggtcgg
      601 gttcgattcg agcgtggtga cgttccctgc gaatccgaac acgctggcga cagttggtga
      661 cggtacgccg acatcaccga aggcgcgggg tgcttcgggt gttggtgtcg agctggcgcg
      721 tgcagagtgg taccggctgg ctgggtagcg agtatcgcct acaagccggg ctggcggttc
      781 cgtgtcggcg gccccggcaa ccggttcctg tgcgtgttcg ctgagactcc agacagcttg
      841 caccctgagc ggactctggt aacgcagcac atgttcgagc taccaccgac ggacgacccg
      901 aaggaacgtg cccgttggct gttgggcgtg ttgctgttgg cggagcggca tgaggcgtgc
      961 gagttcctgt cgttgggtgg tcggcgtccg ttctggccga accatcagga cgagggctgt
     1021 ccgtatgagc tggtggagcg gtgggatggc taagcagtct ccgcacaagc gctggaaggg
     1081 ctgcgcgatg tgcaagtggt acaagttcaa ggacaacggc caggcccaac gccagccgct
     1141 cgcagtgctg cggctagcgg gccgtgaccg tcgcctaaag cgccacgaga tccaccaaga
     1201 cggctggtga cgctgccggg tgacgtgcat gtcgctaccc gcccgctaca atccgagcta
     1261 agcgatcgcg caggctgagc gcagctggtt gcgcagcccc acctagcggg gcacccgacc
     1321 ggcacccgaa gccacccgtg agcggatgac aaaccgtttc cgagtagcac aggagtgcca
     1381 gtcgtggatt tcctcgacct gctccgcgag cgcctgtccg gcatgctcgc cgagcgcagc
     1441 aagatcgacg agcagaagct gaacctcgac gagaagttcg agggcctgat ggcggaagcc
     1501 cgccgtgatg gccgcaacga actcaccgat gacgaggccg caaccatcga agccatgaag
     1561 accgagcgca aggccctcga cgagcggcgc gccaagctcg atcaggacat cgccgacgcc
     1621 gagcagcgtg aggccg
//
LOCUS       DAOQYE010000553         1636 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_91823_length_1636_cov_2.177736, whole genome shotgun sequence.
ACCESSION   DAOQYE010000553 DAOQYE010000000
VERSION     DAOQYE010000553.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91823_length_1636_cov_2.177736"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1636
                     /locus_tag="PKN52_08855"
     CDS             <1..>1636
                     /locus_tag="PKN52_08855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00099.1"
                     /translation="VTGGGRASLTTRPLATELVLDLTGRLGDNVLHLAGTAPDDLRLN
                     LSGPAGQLDGRLAWEAAPGSQPSARLAGTFAGAPLDLTVRGDGDGGGTLSATYGGATA
                     TASVRTDGVRATTAGGATGNATSSATIVGVAVSAPPGSLAPFGVEATASAAWQDGGVV
                     LDGLDAAVSGLLPTGDARVTLAGRVLDAEIGLAGLALAGTLSAGPDLEPAELTVGPHE
                     LGLAWRDLTAALSAPAPAGTPQALAAAPDPVALLDLSRLRLALGGSASEDDVAALLTL
                     FPAAAEGLDGLRVAVAAMDLGWSRATGFTGDLSATATHSAVPEARAELSVRGNGRLFA
                     DVEVRHERVAGTAAEARLELSDDPLADPGLGGTLTLGLPVATIAPQVGAFAAELGAAL
                     EVGGTLTNPTLTGTARLAGDVAASGPVTFADGRLAAKLDGPALHAQGTAELGASFAWR
                     GEAALTDLDVSDWLAQVTEPRLSLTATLDDAGVTVSDLRLAAPGSLVTGSASLGFGSG
                     GLRVNANAVLDLADIDLGGVTLTGMVRGPVAISAADP"
BASE COUNT          186 a          656 c          606 g          188 t
ORIGIN      
        1 aggtgacggg cggcggccgg gcgagcctca ccacgcgacc gctcgcgacc gagctggtcc
       61 tcgacctcac ggggcggctg ggcgacaacg tactgcacct ggccggcacg gccccggacg
      121 acctccgcct caacctcagc ggtccggcgg gccagctgga cggccgactc gcctgggaag
      181 ccgcgcccgg ctcgcagccg tccgcacgcc tggcgggcac gttcgcgggc gcgccgctcg
      241 acctcaccgt gcgcggcgac ggcgacggcg gcggcacgct cagcgccacc tacggcggcg
      301 cgacggcgac ggccagcgtc aggaccgacg gcgtacgggc cacgacggcg ggcggtgcca
      361 cgggcaacgc tacgagcagc gccacgatcg tcggcgtcgc cgtctccgcg ccgccgggca
      421 gcctcgcgcc gttcggggtg gaggcgaccg cgtccgccgc ctggcaggac ggaggcgtgg
      481 tcctcgacgg cctcgacgcc gccgtgtcag gcctcctgcc cacgggcgac gcgcgcgtga
      541 ccctcgccgg ccgggtcctc gacgccgaga tcggcctggc gggtctggcg ctcgccggca
      601 cgctcagcgc gggacccgac ctggagccgg ccgagctgac ggtcggaccg cacgagctcg
      661 ggctcgcctg gcgcgacctg acggcagcgc tctcggctcc ggcgcctgcg ggaacccccc
      721 aggccctcgc cgcggccccc gacccggtgg cgctcctcga cctctccagg ctgcgcctgg
      781 cgctcggcgg cagcgcatcg gaggacgacg tggcagccct gctcacgctc ttccccgccg
      841 ccgccgaggg cctagacggg ctgcgggtgg cggtcgccgc catggacctc gggtggtcgc
      901 gcgccacggg cttcaccggc gacctcagcg ccacggccac ccattccgcc gtgccggagg
      961 cgcgcgccga gctatcggtc cgtggcaacg gcaggctgtt cgccgacgtg gaggtgcggc
     1021 atgagcgcgt cgccggtacg gcggccgagg cacgcctgga actctcggac gacccgctcg
     1081 ccgatcctgg cctcgggggc acgctcacgc tcggtctgcc ggtcgccacc atagcgcccc
     1141 aggtcggcgc cttcgcggca gagctcggcg ccgcgctgga ggtcggcggc acgctcacga
     1201 accccaccct gaccggcacg gcgcgcttgg cgggcgacgt cgcggcgtcg ggccctgtca
     1261 ccttcgccga cggtcgcctc gcggccaagc tcgacggccc cgccctgcac gcgcagggca
     1321 cggcggagct cggcgcctcg ttcgcatggc gaggcgaggc cgccctgacc gacctggacg
     1381 tgagcgactg gctcgcccag gtgaccgagc cgcgcctgag cctgacggcg accctagacg
     1441 acgcgggcgt gacggtgagc gacctgcgcc tggccgcccc cggctcgctc gtcaccggct
     1501 cggccagcct cggcttcggc tccggcgggc tgcgcgtcaa cgcgaacgcc gtgttggacc
     1561 tggcggacat cgacctgggc ggcgtgaccc tcaccggcat ggtgcgcggg cccgtcgcca
     1621 tctccgcggc cgaccc
//
LOCUS       DAOQYE010000554         1632 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_92213_length_1632_cov_2.148383, whole genome shotgun sequence.
ACCESSION   DAOQYE010000554 DAOQYE010000000
VERSION     DAOQYE010000554.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1632
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92213_length_1632_cov_2.148383"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            150..374
                     /locus_tag="PKN52_08860"
     CDS             150..374
                     /locus_tag="PKN52_08860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00100.1"
                     /translation="MNPRKLSVSVPESVASFVESYKREHALKTKSEVVQRALTLLREQ
                     ELERTYTETAGEISPNWEVTLSDGLTEEAW"
     gene            complement(502..1422)
                     /locus_tag="PKN52_08865"
     CDS             complement(502..1422)
                     /locus_tag="PKN52_08865"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HNR00101.1"
                     /translation="MTLHADELPIDDALVRSLVDRDLPEYVGMTLTPLGASGSTNALF
                     RLGDDLLVRLPRQPGVGASVLKEARLAPLLARALPVAVPEVVAVGNPGFGYTEHWSVT
                     RWLGGEHPRRAEPGKPAKSERLGLAEDLATVVLALRAIDALDVVGDDLRWYRGGALGD
                     FDAAVRQYLELCRRIPGLDLDLARAETIWDEALMLPGAAEPGPDRWYHGDLVAENLLV
                     ADRRLAAVLDLGSVSIGDPTVDLHGAWEVLDGPARAAFAARLGVSEAEWLRGRAWALG
                     IALGALSYYWDSMPGRRDDRLAMARNVLGD"
     gene            complement(1428..>1632)
                     /locus_tag="PKN52_08870"
     CDS             complement(1428..>1632)
                     /locus_tag="PKN52_08870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00102.1"
                     /translation="KTLRKLRALSWSEVHRDKGLRMESIKSLGVDAYTLRVTQRCRAV
                     VKREGEILRFLSLHPDHDSAYRR"
BASE COUNT          313 a          564 c          518 g          237 t
ORIGIN      
        1 acactaaaga gtacgcacat acgcactttt gtgcacaggc gcgactctca cgccgggagg
       61 gccggccact ggcagcgttg ggaagccttg gcggcgtctt ccgccgccga acacctctgc
      121 gtaatccatt ccgtcgcatg atctagggaa tgaatccacg gaagctctcg gtatcggttc
      181 ctgaatccgt cgccagcttc gtcgagtcat acaaacgcga gcacgccttg aagaccaagt
      241 ccgaggtcgt gcagcgcgcc ttgactttgc tcagggagca agaactcgag agaacctaca
      301 cggaaacggc cggcgagatc agcccgaact gggaggtcac gctttcagac ggactcactg
      361 aagaggcttg gtagtggcaa tttggcagcg tcgcactacc gggctccgtg gagcgtccga
      421 gcagcgctta agccaaccag gcgccaaccg tcagcaagga agctcggtca tagctcggtg
      481 acaagatcac gctgccaggc gtcagtcgcc aagcacgttc cgcgccatcg ccaaccgatc
      541 gtctcgacga ccgggcatgc tgtcccagta gtaagacagc gcaccgagcg cgatccccag
      601 cgcccacgcc cgcccgcgca accactcggc ctcgctgacg cccagccgcg cggcgaacgc
      661 cgcgcgcgcc ggcccgtcaa ggacctccca agcgccatga aggtcgacgg tcgggtcgcc
      721 gatagagacc gagccgaggt ccaacacagc cgctaggcgg cggtcggcca ccagcaggtt
      781 ctccgcgacg aggtcgccgt ggtaccagcg atccgggccg ggctcggcgg cgccgggtag
      841 catcagagcc tcgtcccata tggtctcggc cctagccagg tccaggtcta ggcctgggat
      901 gcgccggcac agttccaggt actggcgcac ggcagcgtcg aagtcgccca gcgcaccgcc
      961 ccgataccag cgcagatcgt ctcccaccac gtcgagagcg tcgatggcgc gtaaggcgag
     1021 gaccacggtc gcgagatctt ccgcgaggcc caagcgctcg ctcttggccg gcttgcccgg
     1081 ctcggctcgg cgcggatgct cgccacccag ccaccgggtc accgaccagt gctccgtata
     1141 accgaagcca gggttgccga cggccaccac ttcgggcacc gccacgggga gggcccgcgc
     1201 cagcagcggc gctaggcggg cttccttgag gacactcgcg ccgacgccgg gctgcctggg
     1261 cagccggacc agcaggtcgt cgcccagccg gaacaacgcg ttggtcgagc cggacgcgcc
     1321 caacggagtc aacgtcatgc ccacgtactc gggcaggtcg cggtcgacca gggaccgcac
     1381 cagcgcgtcg tcgatcggga gttcgtcggc atggagggtc atgaacgcta tcgacggtaa
     1441 gcagagtcgt gatcaggatg cagagacaag aaccgcagga tctcgccctc gcgcttcacg
     1501 acggcgcgac accgctgggt gacccgaagc gtgtaggcgt cgaccccaag ggacttgatg
     1561 ctctccatgc gcaacccctt gtcgcgatgg acctccgacc agctcaacgc cctcagcttg
     1621 cgaagggtct tg
//
LOCUS       DAOQYE010000555         1630 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_92342_length_1630_cov_3.007619, whole genome shotgun sequence.
ACCESSION   DAOQYE010000555 DAOQYE010000000
VERSION     DAOQYE010000555.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92342_length_1630_cov_3.007619"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="PKN52_08875"
     CDS             complement(<1..420)
                     /locus_tag="PKN52_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HNR00103.1"
                     /translation="MSAEASSHAATGAAAHPAPYRSSKLPDVKLLMWVFLASDTMFFG
                     SLIGTYLAYRERSLVGPYPKDLFDIPLTTVSTFVLLMSSFLMVLALHALRQNDIGKFR
                     LWTAGVAFFGAIFLGFQVFEFTHFAHAGLSIHQNLFGS"
     gene            complement(417..>1630)
                     /locus_tag="PKN52_08880"
     CDS             complement(417..>1630)
                     /locus_tag="PKN52_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HNR00104.1"
                     /translation="LELILDRTFGTLFFVQEAGALPILWQHLFWIFGHPEVYIIILPA
                     FGVISEIIPTFSRKSLFGYPVMILSGILIGFMGAAVWTHHMFTTGLGPVVNTAFSLTS
                     FVIGIPTGIKIFNWLGTLWGGSIKFTTPLIYAIAFLITFTLGGITGIMLAMPTFDAQV
                     HDTYFVVAHFHYVMGGGALLSYLGALYYWFPKMTGRMMSERLGKIAAFVTVIGFHGIF
                     FPQHIAGLLGMPRRIQTYPAGIGLELWNAMSTMGSWLMGIGLILIIINVIHGLTRGKR
                     VGNDPWDARTLEWETSSPPPYYDFEVQPVVNSLDPVWARKHPDAMHVTPPAPDAGAKY
                     DAGGVHIPGQSWYPFLASAAIMFGCYALMYDNFILASICGLFFIFTIFGWAFEGVGGH
                     HVHFDGAKEHA"
BASE COUNT          321 a          454 c          600 g          255 t
ORIGIN      
        1 cgagccgaag aggttctggt ggatgctgag gcccgcgtgc gcgaagtgcg tgaactcgaa
       61 gacctggaac ccgaggaaga tcgccccgaa gaaggcgacg ccggcggtcc agaggcggaa
      121 cttgccgatg tcgttctggc gtagcgcgtg cagcgccaag accatcagga agctcgacat
      181 gagcagcacg aacgtgctga ccgtggtcag ggggatgtcg aagaggtctt tcgggtaggg
      241 gccgacgagg ctgcgctcgc ggtaggcgag gtacgtgccg atgagcgacc cgaagaacat
      301 cgtgtcggac gcgaggaaca cccacatcag gagcttgacg tcgggcagct tggaggagcg
      361 gtacggcgcc gggtgggcgg cggctccggt ggcggcgtgg ctggaagctt cggcgctcat
      421 gcgtgctcct tggcgccgtc gaagtgcacg tggtgaccgc cgacgccttc gaacgcccac
      481 ccgaagatcg tgaagatgaa gaacaggccg cagatgctgg cgaggatgaa gttgtcgtac
      541 atgagggcgt agcagccgaa catgatggcg gcgctggcca ggaacgggta ccaggactgg
      601 ccggggatgt ggacgccgcc tgcgtcgtac ttcgctccgg cgtcaggggc cggtggcgtc
      661 acgtgcatgg cgtcggggtg cttgcgagcc cacacggggt cgagcgagtt gacgacaggc
      721 tgcacctcga agtcgtagta gggcgggggg gacgaggtct cccattcgag cgtgcgggcg
      781 tcccatgggt cgttgccgac gcgcttgccg cgcgtgagcc cgtggatgac gttgatgatg
      841 atcaggatta ggccgatgcc catgagccag ctgcccatgg tcgacatggc gttccacaac
      901 tctaggccga tgccggccgg gtaggtctgg atgcgccgcg gcatgccgag gagccccgcg
      961 atgtgctgcg ggaagaagat gccgtggaaa ccgatcacgg tgacgaacgc cgcgatcttg
     1021 ccaaggcgct ccgacatcat cctgccggtc atcttgggga accagtagta cagcgccccg
     1081 aggtacgaga gcagcgcccc accgcccatc acgtagtgga agtgcgcgac gacgaagtag
     1141 gtgtcgtgca cctgcgcgtc gaacgtcggc atggcgagca tgatccccgt gatgccgccc
     1201 agcgtgaacg ttatgaggaa cgcgatcgcg tagatgagcg gcgtggtgaa cttgatactg
     1261 ccgccccaca gcgtgccgag ccagttgaag atcttgatgc cggtcggtat gccgatcacg
     1321 aaggatgtca gggagaacgc cgtgttcacg acggggccga gacccgtggt gaacatgtgg
     1381 tgcgtccaga cggccgcgcc catgaagccg atcaggatgc ccgagaggat catcaccggg
     1441 tagccgaaga gcgacttgcg cgagaacgtc gggatgatct cgctgatgac cccgaacgct
     1501 ggcaggatga tgatgtacac ctcggggtgc ccgaagatcc agaagaggtg ctgccacagg
     1561 atgggcagcg ccccggcctc ctgcacgaag aagagcgtgc cgaaggtgcg gtcgaggatg
     1621 agctcgagga
//
LOCUS       DAOQYE010000556         1630 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_92371_length_1630_cov_2.359365, whole genome shotgun sequence.
ACCESSION   DAOQYE010000556 DAOQYE010000000
VERSION     DAOQYE010000556.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92371_length_1630_cov_2.359365"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="PKN52_08885"
     CDS             <1..448
                     /locus_tag="PKN52_08885"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNR00105.1"
                     /translation="VTIMVPTQTNLMLAIGLSFVPGMARLVRAQVLSVKDLEYVAAVR
                     AQGASAARVLIGHVLPNAIGPVIASFVMYIPGGIMTISGLGFIGLGIQPPTPEWGAML
                     ASGRAYIRDAWHITTLPGLAIVMTIIALTLVGDGLRDAIDSRMSNR"
     gene            535..>1630
                     /locus_tag="PKN52_08890"
     CDS             535..>1630
                     /locus_tag="PKN52_08890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HNR00106.1"
                     /translation="MEAAPKEAPPTLTFNHYRSGVYEGKFVFGGNELLWHGVDPQTRM
                     QLKRVPFEKSMKESLFDLPSLHANFAALSSAAWSGESVTLDNGTRYNRYLAFPGTVDG
                     RGATGQLWAMRGEPPIMDVVTVEGKVVAFVMPGRISSEMLIRDGHESVTPFAKYADPQ
                     LSKVEYGIRPLGNQAVRTRDGVNLATEVFLPAGGQPGQRFPTIVVRTCYGKARDLNRC
                     THWVNRGYAFVIQDVRGRSDSDGELVPFYHEREDARDLFDWIAAQEWSDGNIGMWGAS
                     YLGYTTTAAATTGHPSLKTAISEVNVGSPFYYDTVRRGGAVCSWPLLCWTLGQSVSNR
                     TDFDVFGGKTVDPEKVVRMRPLLDIPTQAIG"
BASE COUNT          278 a          573 c          538 g          241 t
ORIGIN      
        1 cgtgaccatc atggtgccca cccagacgaa cctaatgctc gccatcggcc tgagcttcgt
       61 gcccgggatg gcacgcctcg tgcgggccca ggtgctctca gtgaaggacc tcgagtacgt
      121 ggcggccgtc agggcccagg gcgcgagcgc cgcgcgggtc ctcatcgggc acgttcttcc
      181 caacgccatc ggacccgtga tagccagctt cgtcatgtac atcccgggtg ggatcatgac
      241 catcagcggc ctggggttca tcggcctcgg catccaaccc cccaccccgg agtggggcgc
      301 catgctcgcg agcgggcgcg cctacatccg agacgcatgg cacatcacga ccctgcccgg
      361 cctcgcgatc gtcatgacga tcatcgccct gacgctggtc ggcgatgggc tcagggacgc
      421 catcgactcg cggatgtcca accggtgagc ccgtcgatac ctgccgccac gagcggcgca
      481 tggagcaagc ctgcggccgg gagcaccgaa gccgcacgta tggaggccgc acgcatggaa
      541 gcagcaccga aggaagcccc gcccaccctc acgttcaacc actaccgctc cggcgtctac
      601 gagggcaagt tcgtgttcgg cggcaacgag ctgctctggc acggggtcga cccgcagacg
      661 cgcatgcagc tcaagcgcgt ccccttcgag aagagcatga aggagtcgct cttcgacctc
      721 ccttccctac atgcgaactt cgccgccctc tcgtcggccg cgtggagcgg cgagagcgtg
      781 acgctcgata acggcacccg ctacaaccgc tacctggcgt tcccggggac ggtcgacggg
      841 cggggggcga cggggcagct ctgggcgatg cgcggcgaac cgcccatcat ggacgtcgtc
      901 acggtggaag gcaaggtcgt cgccttcgtg atgccagggc gcatatcgag cgagatgctc
      961 atccgcgacg gccacgagag cgtgacgccg ttcgcgaagt acgccgaccc gcaactgtcg
     1021 aaggtcgagt acgggatccg cccgttgggc aaccaggccg tccggacgcg cgacggtgtg
     1081 aacctcgcca ccgaggtctt cctcccggcc ggcggtcaac cgggccagcg cttcccgacg
     1141 atcgtcgtgc gcacgtgcta cggcaaggcg cgcgacctca accgctgcac ccactgggtg
     1201 aaccgcggct acgccttcgt catccaggac gtgcgggggc gctcagactc ggacggcgag
     1261 ctcgtgccct tctatcacga gcgcgaggac gcccgcgacc tgttcgactg gatcgccgcg
     1321 caggagtgga gcgacggcaa catcggcatg tggggcgcca gctacctcgg ctacacgacg
     1381 acggccgcgg cgacgacagg ccacccgagc ctcaagaccg ccatcagcga ggtgaacgtc
     1441 ggctcgccct tctactacga cacggtgcgc cgcggcggcg ccgtgtgctc gtggccgctg
     1501 ctgtgctgga cgctcggcca gtccgtgtcc aacaggacgg atttcgacgt gttcggcggc
     1561 aagaccgtcg acccggagaa ggtcgtgcgc atgcgaccgc tgttggacat ccccactcag
     1621 gcgatcggga
//
LOCUS       DAOQYE010000557         1628 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_92586_length_1628_cov_1.824539, whole genome shotgun sequence.
ACCESSION   DAOQYE010000557 DAOQYE010000000
VERSION     DAOQYE010000557.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1628
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92586_length_1628_cov_1.824539"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1213)
                     /locus_tag="PKN52_08895"
     CDS             complement(<1..1213)
                     /locus_tag="PKN52_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00107.1"
                     /translation="MARPPKSSDLLTMHTVSDVQVSADGRLAAWVQTDVVPAVGDPTV
                     AGGKPYAPPRYRSRIHLRPLSNLARRQPTEFTRGEYGDRAPRFSPDAAKLAFLRSSEA
                     GSPPQLHVMPVGGGEPERLTSHKAGVFAFAWAPGGERIAYLSLGEHEEVAAKNGQPRR
                     IVRRYWRGDGAGVIAQATAQLYLLDLAKGEPRRVTDLAESPRLLAFAPDGESVWLAVP
                     SGERAAGEFRADIVLFELSTGAASVRAKDLIGLSSLQPSPDGSWLAYTASQRQDDVAA
                     EGGVWLLDARAERAKPRLLTGDVATPPSAGGDSRLGAYPFGPTWSADSSALTVLVNDA
                     GASGLARLTLDGALAPLAAPATIAASGRVVTAFATPAAPGDVTALLISETPDRPGEVS
                     ALLAGGREARLS"
     gene            complement(1262..>1628)
                     /locus_tag="PKN52_08900"
     CDS             complement(1262..>1628)
                     /locus_tag="PKN52_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNR00108.1"
                     /translation="VVMEERRSAPLTAVVVKAAAGATAHVPLVVVTNLPRTIEELKER
                     GVWVYGADAAATSVPGEVDWRRPVALVIGSEGDGMRRLVRERCDELVAIPLSGKVGSL
                     NAGVAAGILLYAIGVGRGR"
BASE COUNT          203 a          581 c          624 g          220 t
ORIGIN      
        1 ggctgagccg cgcttcgcgc ccgccggcga ggagcgcgct cacctcgccg gggcggtcgg
       61 gggtctcgga gatgaggagc gccgtcacgt cgcctggcgc cgccggcgtc gcgaacgccg
      121 tgacgacccg accgctcgcc gcgatggtgg ccggtgccgc gagcggtgcc agcgcgccgt
      181 cgagcgtgag gcgcgccagg ccgctcgcgc cggcgtcgtt gacgagcacg gtgagagccg
      241 agctgtcggc gctccacgtc ggtccgaacg gataggcccc gaggcggctg tcgccgccgg
      301 cgctcggggg cgtcgcgacg tcgcccgtga ggaggcgggg cttggcgcgt tcggcgcggg
      361 cgtccaggag ccagacgccg ccttcggcgg ccacgtcgtc ctgacgttgg ctggccgtat
      421 aggccagcca ggagccgtcc ggcgacggtt gcagggagga cagcccgatg aggtccttgg
      481 cgcggacgct ggcggcgccc gtgctcagct cgaagagcac gatgtcggct cggaactcgc
      541 ccgccgcccg ctcgccggag ggaacggcca gccatacgga ctcgccgtcc ggcgcgaacg
      601 cgagcaggcg cggcgactcg gcgaggtcgg tgacccgacg cggctcgccc ttggctaggt
      661 cgaggaggta gagctgcgcc gtcgcctgcg cgatgacgcc agcgccgtcg ccgcgccagt
      721 agcgcctgac gatgcgccgc ggctggccgt tcttggcggc gacttcctcg tgctcaccga
      781 ggctcaggta ggcgatccgc tctccgcccg gggcccaggc gaacgcgaac acgccggcct
      841 tgtggctggt gagccgctcc ggctcacctc cgccgaccgg catgacgtgc aactgcggcg
      901 gagagccggc ctccgagctg cgcaggaacg cgagcttggc ggcgtcgggg gagaagcgcg
      961 gcgccctgtc gccgtactcg ccgcgcgtga actcggtggg ttggcgcctc gcgaggttac
     1021 tcagcggccg gaggtggatc cggctccggt agcggggcgg ggcgtaaggc ttgccgcccg
     1081 ccacggtcgg gtcgccgacg gcggggacga cgtcggtctg cacccacgcc gccaggcggc
     1141 cgtcggccga gacctgcacg tcggagaccg tgtgcatcgt gaggaggtcg ctgctcttcg
     1201 gtggtcttgc catgtagtta cgaccgttcc gcgccgcgac ggcgcatgtg attcctggcg
     1261 gtcagcgccc gcgaccgacg ccgatcgcgt acaacaggat gccggcagcc acgcccgcgt
     1321 tcagcgagcc gaccttgcct gacaggggga tggccacgag ctcgtcgcac cgctcgcgca
     1381 cgaggcgccg catcccgtcg ccttcggagc cgattaccag ggctacgggg cggcgccaat
     1441 cgacctcgcc gggcacgctc gtggctgccg cgtcggcgcc gtagacccac acgccgcgtt
     1501 ccttgagctc ctcgatcgtg cggggcaggt tcgtcacgac gacgagtggc acgtgcgccg
     1561 tcgcgcccgc ggccgccttg acgacgaccg ccgtgagggg ggcggagcgg cgctcttcca
     1621 tcaccacg
//
LOCUS       DAOQYE010000558         1627 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_92655_length_1627_cov_2.183842, whole genome shotgun sequence.
ACCESSION   DAOQYE010000558 DAOQYE010000000
VERSION     DAOQYE010000558.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92655_length_1627_cov_2.183842"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="PKN52_08905"
     CDS             complement(<1..298)
                     /locus_tag="PKN52_08905"
                     /inference="COORDINATES: protein motif:HMM:NF015427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HNR00109.1"
                     /translation="MTERIQPLMYELETLERSLSDPAILADGTEYRRVLKRYGEVKGI
                     VDAASDVARLEGQLAGLSELLTDPELRSEAEDEAERLEQSVAKRRAELAELLTPR"
     gene            complement(390..>1627)
                     /locus_tag="PKN52_08910"
     CDS             complement(390..>1627)
                     /locus_tag="PKN52_08910"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNR00110.1"
                     /translation="DSCRAHHTPLPPRCTVRRFLVTTSFVVALLAAPPGVALAASELT
                     VGLIASRNQPAGAAQTLVGTAWAAEVRASGGVFGTPVRLLTADDGGDPQRAAELATEQ
                     ISEGALVIVCCTTAAASRLVAAVAESAGVTLLAPSPYEQASRPGYWAFSLAPSDTDAL
                     AAIVADAYKKGRTSLALLAPDLPLAAAASEQLVALGGIVGIRLMADERYRAGQVELRP
                     EALLVASYRPGAVVVLGFADDLENAASALRRRGYEGDVYGRSLPFFPGGELPAAARVA
                     DMLVAAPPVAALAGNDRTGLTYPDGSEALGPCTEVAIRDAARLAVAPGALRNALSTAP
                     VLGGLDLVRSAFEQVLALQIPANDERVLRQAVRDALVGLPATCTGAGSLDLEDGRASA
                     VQPRGLAIVRVTQTGLAIP"
BASE COUNT          228 a          575 c          600 g          224 t
ORIGIN      
        1 cgcggggcgt gagcagctcg gccagctcgg ccctgcgctt ggctacggac tgctccagac
       61 gctcggcctc gtcctcggcc tcgctcctga gctccgggtc tgtcaggagc tcggacaggc
      121 cggcgagctg gccctcgagg cgggcgacgt cggaggcggc atcgacgatg cccttgacct
      181 cgccgtaacg cttgagcacg cgccgatact cggtgccgtc cgccaggatg gcggggtccg
      241 agaggcttcg ctccagcgtc tcgagttcgt acatgagggg ttggatacgc tcggtcattg
      301 tcccaagtgt actctccggg ccgcgccgcg cccggatgcc ttaccgaacc ggtatagata
      361 gaccggtcag tcggctgatc ttccgctggt cacgggatgg caagaccggt ctgtgtcacc
      421 ctcacgatcg cgagtccgcg cggctgcacc gcgctggcgc gcccgtcctc caggtcgagg
      481 ctgccggcgc ccgtgcaggt cgccgggagg cccaccagcg cgtccctgac cgcctggcgt
      541 agcacgcgct cgtcgttggc cgggatctga agggccagga cctgctcgaa cgccgagcgc
      601 accaggtcga ggccgccgag gaccggcgcg gtcgagagcg cgttgcgcag ggcgccgggg
      661 gcgaccgcca gccgggccgc gtcgcggatc gcgacctccg tgcacggtcc aagggcttcc
      721 gaaccgtccg ggtaagtcaa gccggtgcgg tcgttccccg ccaaggcggc gacgggtggg
      781 gccgctacca gcatgtcggc cacccgggcg gcggccggca gctcgccgcc ggggaagaac
      841 ggcaggctcc tgccgtagac gtcgccctcg tagcccctgc gccgtagcgc cgaggccgcg
      901 ttctccagat cgtccgcgaa gcccaagacg accaccgcac caggccggta gctggccacc
      961 aggagcgctt ccggcctgag ctccacttgg ccggcccgat agcgctcgtc ggccatgagc
     1021 ctgatgccta cgatgccgcc aagcgcgacc aactgctcac tcgccgcggc ggccaggggt
     1081 aggtcgggcg ccaggagcgc gagcgacgtg cgccccttct tgtaggcgtc ggccacgatg
     1141 gccgccaggg cgtccgtgtc ggagggggcg agcgagaagg cccagtagcc gggccggctc
     1201 gcctgttcgt aaggcgaggg cgccaacagc gtgacgccgg ccgactccgc gaccgccgcc
     1261 accaggcggc tggcggcggc ggtggtgcag cagacgatga ccaacgcgcc ctcggagatc
     1321 tgctccgtgg cgagttcggc cgcgcgctgc gggtcgccgc cgtcgtcggc cgtcaggagc
     1381 ctgaccgggg tgccgaagac gccgccgctt gcgcgcacct ccgccgccca cgccgttccc
     1441 acgagcgtct gcgcggctcc ggccggttgg ttgcgggagg cgatcagacc gacggtgagc
     1501 tcagaggcgg cgagagccac gccgggcggc gccgcgagga gcgcgactac gaacgaagtt
     1561 gtgaccagga acctgcgcac tgtgcacctt ggtgggagag gagtgtggtg cgcccgacag
     1621 gagtcga
//
LOCUS       DAOQYE010000559         1623 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_92984_length_1623_cov_3.232143, whole genome shotgun sequence.
ACCESSION   DAOQYE010000559 DAOQYE010000000
VERSION     DAOQYE010000559.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92984_length_1623_cov_3.232143"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1441)
                     /locus_tag="PKN52_08915"
     CDS             complement(<1..1441)
                     /locus_tag="PKN52_08915"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HNR00111.1"
                     /translation="MGVTVPVDRPQGERGAGLRVNDFSIVVATANGTGSQTANLTLLR
                     SFFKMGIPVHGKNIFPSNIQGLPTWYHIRVNKDGYIARKPSEFLVAFNPATLHEDVAT
                     LPSGGVVVHNLDLHLKPERADLTYYPVPVKQLLAGVEVKGKIKEYIANMTYVGVVAHL
                     LGVPLPVIEEALEHHFGNRRKLVDSNLEVVKIAHAWAVENLAKNDQFSVEPMNATEGL
                     IMMTGNEAGALGAVFGGASVAGWYPITPSTSFIDAFREYLAKLRHDVDGRPTYTVIQA
                     EDELAAIGMVVGAGWAGARAVTATSGPGISLMAEFLGLAYYAEVPVVVWDIQRVGPST
                     GLPTRTSQGDVAFSYYLGHGDTKQVLLFPSSIEECFEFGYRAHDLADALQTPVLVLSD
                     LDLGMNNWMGEPFNYPDAPLNRGKVLSAEEVAALPDFGRYKDVDGDGVPYRTIPGNEN
                     PRAAWFGRGTGHDENARYSERADHWVQNMD"
BASE COUNT          239 a          516 c          577 g          291 t
ORIGIN      
        1 ggtccatgtt ctgcacccag tggtcggcac gctcggagta cctggcgttc tcgtcgtggc
       61 cggtgccgcg gccgaaccag gcggcgcgcg ggttctcgtt gcccgggatg gtgcggtacg
      121 ggacgccgtc gccatccacg tccttgtagc gaccgaagtc tggcagcgcg gccacctcct
      181 ccgccgagag gaccttgccc cggttcaggg gcgcgtccgg gtagttgaac ggctcgccca
      241 tccagttgtt catgccgagg tcgaggtcgg agagcacgag gacgggggtc tggagcgcgt
      301 ctgccaggtc gtgggcgcgg taaccgaact cgaagcattc ctcgatggac gacgggaaga
      361 ggaggacctg cttcgtgtcg ccgtggccga ggtagtagga gaaggcgacg tcgccttggc
      421 tcgtgcgcgt cggcaggccc gtgctggggc caacgcgctg gatgtcccac acgacgacgg
      481 ggacctcggc gtaataggcg aggcccagga actcggccat cagcgagatg ccggggccgc
      541 tcgtcgccgt gacggcgcgg gcgcccgccc agccggcgcc gacgaccatg ccgatggccg
      601 ccagttcgtc ctcggcctgg atgaccgtgt aagtcgggcg gccgtccacg tcgtggcgca
      661 gcttggctag gtactcgcgg aacgcgtcaa tgaagctcgt ggagggggtg atggggtacc
      721 agccggccac gctcgcgccc ccgaagacgg cgccgagcgc acccgcctcg ttgcccgtca
      781 tcatgatcag cccctcggtc gcgttcatgg gctcgacgct gaactggtcg ttcttggcga
      841 ggttctccac cgcccaggcg tgggctatct tcacgacctc gaggttgctg tcgacgagct
      901 tcctgcggtt gccgaagtgg tgctcgaggg cctcctcgat gacggggagt ggcacgccga
      961 ggaggtgcgc gacgaccccg acgtacgtca tgttggcgat gtactccttg atcttgccct
     1021 tgacctcgac cccggcgagg agttgtttga cgggcaccgg gtagtacgtg aggtcggccc
     1081 gctccggctt gaggtgcagg tccaggttgt ggaccacgac cccgccgctc ggcagggtgg
     1141 ccacgtcctc gtgcagggtg gcggggttga acgcgacgag gaactcggat ggcttgcggg
     1201 cgatgtagcc gtccttgttg acgcggatgt ggtaccaggt tggcaggccc tggatgttcg
     1261 atgggaagat gttcttcccg tggaccggga tgcccatctt gaagaacgac ctcagtagcg
     1321 tgaggttggc ggtctggctg cccgtcccgt tggcggtcgc gacgacgatg gagaaatcgt
     1381 tgactctcag gcccgcgccg cgctctcctt gcgggcggtc cacaggaacg gttacaccca
     1441 tgcgctcacg cacctccagg gaaggtctcg ctcaccgcgc ttcccggtcg ggaccgctta
     1501 cctcccggcg cggaaagccg gcgcgacttc ccgttagtca ggttgacggg ttgccccatc
     1561 tactagcgcc atagtaccgc catacccggc cgcgcggcgc acctggaagg ggctcatacg
     1621 cca
//
LOCUS       DAOQYE010000560         1622 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_93064_length_1622_cov_3.407786, whole genome shotgun sequence.
ACCESSION   DAOQYE010000560 DAOQYE010000000
VERSION     DAOQYE010000560.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93064_length_1622_cov_3.407786"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            367..>1622
                     /locus_tag="PKN52_08920"
     CDS             367..>1622
                     /locus_tag="PKN52_08920"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF006126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside triphosphate reductase"
                     /protein_id="HNR00112.1"
                     /translation="MVDAVSSIDEYLDRTDWRVNANANQGYSLGGLILNISGKVVANY
                     WLTSVYPPEIGNAHREGDIHIHDLDMLAGYCAGWSLRTLMTEGLNGVPGKVEAGPPKH
                     LSSAVGQIVNFLGTLQNEWAGAQAFSSFDTYMAPFVRKDRLDYAAVLQSIQELIYNLN
                     VPSRWGTQTPFTNLTFDWTCPEDLRDDVPVIGGEEMPFTYGDLQTEMDMINRAYMEVM
                     TRGDAKGRVFTFPIPTYNIAPDFPWDSENADRLFDMTAKYGLPYFQNFINSELKPNMI
                     RSMCCRLQLDLTELLKRGNGLFGSAEQTGSIGVVTVNCARLGYLHRGDEAGLFERLDT
                     LLELGRDSLEIKRRVVQRYMDEGLFPYTKRYLGTLRNHFSTLGVNGVNEMIRNFTYDG
                     EDITTPWGHAFAVRLLDHVRARIKSF"
BASE COUNT          303 a          569 c          492 g          258 t
ORIGIN      
        1 cacggctcgc gtgttcggct gggtcgcggg tgacgagcag gcggcgctga aggagcgcgg
       61 cgtcatctga cgcgcacggc tagccgcctc cgagcgatcg ccccttagcg caagggccgc
      121 agcaggccgc cctgctaccc ctgcctgccg agtggaatag cgcgcactcg gccccgttat
      181 acctacgcac taggccaccc attgaccatt cggggccccc agatactata tgtagtatct
      241 agctcctccg gttgcccaag gaccacctac gcagcccgtc gagcaccgat cgccacgcga
      301 atggggttgc atatgccact acgacctgat ctgcctcctg agatgccgct gggcgcgcga
      361 ggggccatgg tcgacgccgt ctcgtccatc gacgagtacc tcgaccgcac agactggcgg
      421 gtcaacgcga acgccaacca gggctactcg ctcggcgggc tcatcctcaa catctccggc
      481 aaggtcgtcg ccaactactg gctgaccagc gtctacccgc ccgagatcgg caacgcccac
      541 cgcgaaggcg acatccacat ccacgacctg gacatgctgg cgggctactg cgccggatgg
      601 tcgctgcgca cgctcatgac cgaggggctc aacggcgtgc cgggcaaggt cgaggccggt
      661 ccacccaagc atctgtcgag cgcggtcggt cagatcgtca acttcctcgg cacgctgcag
      721 aatgagtggg cgggggccca ggccttcagc tcgttcgaca cgtacatggc ccccttcgtg
      781 aggaaggacc ggctcgacta cgccgccgtg ttgcagtcga tccaggagct catctacaac
      841 ctgaacgtgc cgtcgcgctg gggcacgcag acgcccttca ccaacctgac gttcgactgg
      901 acctgcccgg aggacttgcg agacgacgtg ccggtcatcg gcggcgagga gatgcccttc
      961 acctacggcg acctccagac cgagatggac atgatcaacc gcgcctacat ggaggtcatg
     1021 acgcggggcg acgccaaggg gcgcgtcttc accttcccca tccccaccta caacatcgcc
     1081 ccggacttcc cgtgggacag cgagaacgcc gaccgcctgt tcgacatgac cgccaagtac
     1141 ggcctgccgt acttccagaa cttcatcaac tcggaactga agccgaacat gatccgctcg
     1201 atgtgctgcc gcctgcagct agaccttacg gagttgctga agcgcggcaa cggcctgttc
     1261 ggcagcgccg agcagaccgg gtccatcggc gtcgtgacgg tcaactgcgc ccgcctaggc
     1321 tacctgcacc ggggcgacga ggcgggcctg ttcgagcgcc tcgacacgct gctcgaactg
     1381 ggccgcgaca gcctggagat caagcgcagg gtcgtgcagc gctacatgga cgaggggctg
     1441 ttcccctaca ccaagcggta ccttggcacc ctcaggaacc acttctccac cctcggcgtc
     1501 aacggcgtga acgagatgat ccgcaacttc acctacgacg gcgaggacat cacgacgccg
     1561 tggggccacg cgttcgcggt gcggctgctc gaccacgtgc gtgcgcgcat aaagtcgttc
     1621 ca
//
LOCUS       DAOQYE010000561         1620 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_93276_length_1620_cov_3.028115, whole genome shotgun sequence.
ACCESSION   DAOQYE010000561 DAOQYE010000000
VERSION     DAOQYE010000561.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93276_length_1620_cov_3.028115"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="PKN52_08925"
     CDS             <1..288
                     /locus_tag="PKN52_08925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00113.1"
                     /translation="QAAARELAEEVQLGGDLALVSRLYCSPGFCDEQVYLFELTTMRQ
                     AAGTPDEGEVLTLEWRDALDVWNSVRSGAEATSGVTLLGLRHVLARSGTPV"
     gene            285..1190
                     /gene="ribF"
                     /locus_tag="PKN52_08930"
     CDS             285..1190
                     /gene="ribF"
                     /locus_tag="PKN52_08930"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HNR00114.1"
                     /translation="MRRRVTSTYGSVPDVPVRGGVLSIGNFDGVHLGHRLLLDRMRAA
                     ALARGVPSVVVTFFPPARVLFGGGQYLMTPEEKLVALSAYAPDVVVSIPFDRDFANTE
                     KETWLAQLSELAPSLIVVGEDFRFGKARAGGLDDLRGIAAELEAFPLLEVDGAVVSSS
                     RVRELLGMGDVANATRLLGAPYLVRGVVTRGQQRGRTIGFPTANLDVPSGKVLPRGVF
                     AVTVEAAGRALAGMANVGPRPTFPEAPPALEVHVFDFADDLYGAELDVRFVKQLRGQR
                     AFAGVEELRAQLSMDERAAREALGL"
     gene            1279..>1620
                     /locus_tag="PKN52_08935"
     CDS             1279..>1620
                     /locus_tag="PKN52_08935"
                     /inference="COORDINATES: protein motif:HMM:NF019644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase substrate-binding
                     domain-containing protein"
                     /protein_id="HNR00115.1"
                     /translation="MWIYGRNAVLEAMRDAQVEQVAVARGVQPAILKELKRAAAAANV
                     RLDEVPRIQLDQVLKTTQHQGVAALLPEVKTVDMDAVFERAARRGEELLVVLLDHLTD
                     PRNVGAIIRSAE"
BASE COUNT          229 a          578 c          577 g          236 t
ORIGIN      
        1 caggcggccg cgcgggaact cgcggaggag gtccagctcg gcggcgacct ggctctcgtc
       61 tcgcgcctct actgctcacc cggcttctgc gacgagcagg tctacctctt cgaactcacc
      121 accatgcgcc aggccgcggg cacgcccgac gagggcgagg tcctcaccct cgagtggcgc
      181 gacgccctgg acgtatggaa cagcgtgcgg agcggagcgg aggcgacgtc cggcgtcacg
      241 ctcctcggtc tcaggcacgt tctggcgcgc agcggcaccc ctgtgtgagg cgccgggtta
      301 ccagcactta cgggtcggtc ccggacgtgc cggtgcgggg cggcgtgcta agcatcggca
      361 acttcgacgg cgtgcacctc gggcacaggc tcctgctcga ccgcatgcgc gcggcggcgc
      421 tggcgcgcgg cgtaccgagc gtggtcgtca cgttcttccc gccggcgcgg gtgctcttcg
      481 gcggcggcca gtacctcatg acgccggagg agaagctcgt cgcgctgagc gcgtacgcgc
      541 cggacgtagt ggtcagcatc cctttcgacc gcgacttcgc gaacacggag aaagagacgt
      601 ggctggcgca actttccgag ctcgcaccga gcctgatcgt ggtcggagag gacttccggt
      661 tcggtaaggc ccgcgcgggc gggcttgacg acctcagggg catcgccgcc gagctggaag
      721 cgttcccgct cctggaggtc gacggcgcgg tggtcagctc ctccagggtg cgtgagctcc
      781 tcggcatggg cgacgtggcc aacgccacgc ggctcctcgg cgccccctac ctcgtgcggg
      841 gcgtcgtcac gcgcggtcag cagcggggca ggaccatcgg cttcccgacc gcgaacctcg
      901 acgtgccttc cgggaaggtg ctgccgcgcg gcgtcttcgc ggtgacggtc gaggcggcgg
      961 gccgggcgct cgccggcatg gccaacgttg gcccgcgccc gacgttcccg gaggcgccgc
     1021 ccgcgctcga agtgcacgtc ttcgacttcg cagacgacct gtacggcgcc gagctcgacg
     1081 tccgcttcgt caagcagttg cgtggccaac gggccttcgc cggcgtggag gagctgcgcg
     1141 cccagttgag catggacgaa cgtgccgccc gggaggcgct cgggctctga cggtctaccc
     1201 cgtcgccggc accgcctacc cccggcccgg cgaccgaccc gcccgaccaa cgaccatcgc
     1261 gcacgtatat tgggtcgcat gtggatctac ggtcgcaacg ccgtgctcga ggccatgcgc
     1321 gacgcccagg tcgagcaggt ggccgtggcc cgcggtgtac agccagccat cctgaaggag
     1381 ctcaagcggg ccgccgccgc ggctaacgtg cgcctagacg aggtcccgcg catccagctc
     1441 gaccaggtgc tcaagacgac ccagcaccag ggggtggcgg cgctgctgcc tgaggtcaag
     1501 accgtcgaca tggacgccgt gttcgaacgg gcggcgcggc gcggcgagga actcctcgtc
     1561 gtgctcctcg atcacctgac ggacccgcgc aacgtcggcg ccatcatccg cagcgccgag
//
LOCUS       DAOQYE010000562         1618 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_93500_length_1618_cov_2.632758, whole genome shotgun sequence.
ACCESSION   DAOQYE010000562 DAOQYE010000000
VERSION     DAOQYE010000562.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93500_length_1618_cov_2.632758"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..720
                     /locus_tag="PKN52_08940"
     CDS             <1..720
                     /locus_tag="PKN52_08940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00116.1"
                     /translation="STTPVAGNLLFQPAVLDYGATTTELTFELTNTGSANLSWSIDYF
                     YDAVDNPGPVPGGDSGAIFYLAGYPGSGTLAPGASTTTGFAIDRDALTEYGQYQVLIG
                     IFVDGTEYDGPILRFYKLPETPAVAGPTVVEASIYVNDDWLVSGSQNSPSLLTSFDFR
                     ASPGQTVVGAWIDVNSNGVIDGGDYVGFHGNLVQVEAGRQYDVPIVVAPYFEGSPTAW
                     PAEWLDTLGGSRPSGGSPSGE"
     gene            902..>1618
                     /locus_tag="PKN52_08945"
     CDS             902..>1618
                     /locus_tag="PKN52_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00117.1"
                     /translation="MVVSDVAPHGGTFIVNGTNLGTSGTAEIGGVTVPTSAWSAGSVT
                     VTVPSTVPDGPQTLTLTNSGGSATVDVFVGADYPTGTLDELIALNLPKGTAVRLGEGT
                     YSSDAGEVVIDNLSLYGQGKDATIVGVAWPSILMYRADVGEHIVLSRLNLEADAAIFL
                     PSAAVAPAVATEAFSLPASPEEALAQFRARSAALLGRAEVQTTQATSLTFKDMTITQA
                     VAGLFATVNMGGAPVVYPGTV"
BASE COUNT          273 a          553 c          516 g          276 t
ORIGIN      
        1 tcgaccacgc ctgttgccgg taacctcctc ttccagccgg cggtgctgga ctacggcgcc
       61 acgaccaccg agctgacgtt cgagctcacg aacaccggct ccgccaacct cagctggtcc
      121 atcgactact tctacgacgc cgtcgacaac cccggtccgg tgcctggcgg cgatagcggc
      181 gccatcttct acctggccgg ttacccgggt tccggcaccc tcgcgcccgg cgcgagcacc
      241 acgaccggct tcgcgataga ccgcgacgct ctcaccgagt acgggcagta ccaggtgctc
      301 atcggcattt tcgtggacgg cacggagtac gacgggccga tcctgcgctt ctacaagctg
      361 cccgagaccc cggcagtagc cggtcccact gtcgtcgagg cgagcatcta cgtcaacgac
      421 gactggctcg tcagcggcag ccagaactcg cccagcttgc tcacgagctt cgacttcagg
      481 gcctcgccag ggcagactgt cgttggggcg tggatcgacg tgaactcgaa cggcgtcata
      541 gacggcggcg actacgtcgg gttccacggc aacctggtcc aggttgaggc aggcaggcag
      601 tacgacgtgc cgatcgtcgt ggcaccgtac ttcgagggca gtccgaccgc ctggccggct
      661 gaatggctcg acacgctggg tgggagtagg ccgtcgggcg ggagtccgag cggcgagtga
      721 gatagcacgc accttgtgcg agtagtagcc gactagcatc gggtacctgc caaaaggagg
      781 taacccgatg aaagtacgga tcccggcact cgcgcttagt gcgctctctg cttgcatgct
      841 gttattggcc gcgtgcggcg ggggaggcgg aacacctacg cctctcccgc ccagcggctt
      901 gatggttgtt tccgacgtcg ccccgcacgg tggcacgttc atagtcaacg gcaccaactt
      961 gggcaccagc ggcacggccg agatcggcgg cgttaccgtc ccgacctccg cgtggagcgc
     1021 gggctccgtc accgttaccg tgccgtccac agtgcccgac ggtcctcaga cgctcacgct
     1081 gaccaactcc ggtggctcgg cgaccgtgga cgtcttcgtc ggggccgatt acccgacggg
     1141 cacgctcgac gagctcatcg ccctgaacct gccgaagggc accgccgtcc gcctcgggga
     1201 gggaacctac tcatcggatg ccggcgaggt ggtcatcgac aacctcagcc tctacgggca
     1261 gggcaaggac gcgacgatag tcggagtagc ttggcctagc atcctgatgt accgcgcgga
     1321 cgtgggtgag cacatcgtcc tcagccgcct gaaccttgag gcggatgccg cgatcttcct
     1381 tccgagcgcc gccgttgctc cggctgttgc gaccgaagcg ttcagcctcc cagcgagccc
     1441 tgaagaggcg ttggcgcagt tccgggcacg ttcggccgca ctgctcggcc gggccgaggt
     1501 acagaccaca caggctactt ccctgacttt caaggacatg acgatcacgc aggcagttgc
     1561 cggcctcttc gccaccgtga acatgggcgg cgccccggtc gtgtacccgg gtaccgtc
//
LOCUS       DAOQYE010000563         1607 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_94582_length_1607_cov_4.129510, whole genome shotgun sequence.
ACCESSION   DAOQYE010000563 DAOQYE010000000
VERSION     DAOQYE010000563.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94582_length_1607_cov_4.129510"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1409
                     /locus_tag="PKN52_08950"
     CDS             <1..1409
                     /locus_tag="PKN52_08950"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNR00118.1"
                     /translation="VGWVHRERHERLAVAFGFQAGLFNIGAPGQMVMGAIFAMLAGIY
                     MPGPRFIVLPVAVLSAALGGGLWGALPGWLKARFGANEVINTILLNFVAASVLLFILS
                     AGNVFAAAAVRIFVVIGVFVVVLAAALLIPVVRNLVKRAPRAAFAVGAVVLLAGCVVA
                     GLPRADDQPITVNMPFKVPGNEPKSRPLNAGARLPQLPAMLGVTRGGVNVVRIDYASL
                     VALLGAVVAAFRLGRIGRLKGWAARLVGALGVGGALYGLGALVGLTGVATAIPPTKLN
                     LSFLIALLMAVFVQVFLFKTRWGYELRAVGFSPRAAEYGGARIGRNTIMAMAISGALA
                     GLTATHYVLGGALEDYALRQSIPTGDGFDGIAVALLGANQPAFIVASAFLFGVLKYGG
                     SVLNITFPDLTRDVVSMILALVVLFIAAKGFLPERLTNPLKWGVRQEPAAEPAAAAAA
                     AAAATAAQVDPTDRAGEG"
     gene            1413..>1607
                     /locus_tag="PKN52_08955"
     CDS             1413..>1607
                     /locus_tag="PKN52_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNR00119.1"
                     /translation="MDTVILLALASSMLRATTPLLFAALGGLFSERSGIVNIALEGII
                     LFGAMAAAVTTQLVEAPYLRA"
BASE COUNT          189 a          601 c          530 g          287 t
ORIGIN      
        1 cggtcggttg ggtgcatcgc gagcggcacg aacgcctggc ggtcgcgttc ggcttccagg
       61 ccggcctctt caacatcggg gctcccggtc agatggtcat gggcgccatc ttcgcgatgc
      121 tcgccggcat ctacatgccc gggccgcgct tcatcgtcct gccggtcgcg gtgctgtccg
      181 ccgcgctcgg cggcggcctg tggggtgcgt tgccgggctg gttgaaagcc aggttcggtg
      241 ccaacgaggt catcaacacg atcctcctga acttcgtggc cgcgtccgtc ctgctcttca
      301 tcctctcagc cggcaacgtg ttcgccgccg ccgccgtgcg catcttcgtc gtcatcggcg
      361 tcttcgtggt cgttttggcg gccgccctcc tcatcccggt cgtcaggaac ctcgtcaagc
      421 gcgcgccgcg cgccgccttc gccgtcgggg cggtagtgct gctggcaggt tgtgtggtgg
      481 ccggcttgcc gcgcgccgat gaccagccca tcacggtgaa catgccgttc aaggtgccag
      541 gcaacgagcc gaagtcgcgt ccgctcaacg cgggcgcgcg gctaccgcag ctcccggcca
      601 tgctcggcgt gacgcgcggc ggggtcaacg tcgttcgcat cgactacgcc agcctcgtgg
      661 cgctcctcgg cgcggtggtg gccgcgttcc ggctcggccg catcgggcgc ctgaagggtt
      721 gggccgcccg cctcgtcggg gcgctcggcg tcggcggcgc cctctacggg cttggcgccc
      781 tcgtcgggct caccggcgtc gctaccgcca tcccgccgac caagctcaac ctctccttcc
      841 tcatcgccct cctcatggcg gtgttcgtgc aggtgttcct gttcaagacg cgctggggct
      901 acgagctgcg cgccgtcggg ttctcgccgc gcgccgccga gtacggcggg gcgcgcatcg
      961 gccgcaacac gatcatggcc atggccatca gtggagcgct ggcgggcctc acggccaccc
     1021 actacgtcct gggaggcgcc ctcgaggact acgccctgcg ccagtcgatc ccgaccggcg
     1081 acggcttcga cggcatcgca gtggcgctcc tcggcgccaa ccaacctgcg ttcatcgtcg
     1141 cttccgcgtt cctcttcggc gtcctcaagt acggcggctc ggtcctgaac atcacgttcc
     1201 ccgacctcac gcgcgacgtc gtgagcatga tcctcgcgct cgtcgtgctg ttcatagccg
     1261 ccaagggttt cctaccggag cgcctaacga acccgctcaa gtggggcgtg aggcaggaac
     1321 cggcggcaga gcccgcggcg gccgcggcgg cggccgcggc ggcgaccgcg gcgcaagtcg
     1381 acccgaccga ccgcgcaggg gagggctgaa ccgtggacac cgtcatcctt ctagccctcg
     1441 cctcctcgat gctgcgcgcc acgactccgc tcctcttcgc ggcgctcggc gggctcttct
     1501 ccgagcgttc cggcatcgtc aacatcgcgc tcgagggcat catcctcttc ggtgccatgg
     1561 ccgcggccgt cacgacccaa ctcgtcgagg cgccgtacct gcgcgcc
//
LOCUS       DAOQYE010000564         1600 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_95252_length_1600_cov_3.616828, whole genome shotgun sequence.
ACCESSION   DAOQYE010000564 DAOQYE010000000
VERSION     DAOQYE010000564.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95252_length_1600_cov_3.616828"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..632)
                     /locus_tag="PKN52_08960"
     CDS             complement(<1..632)
                     /locus_tag="PKN52_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00120.1"
                     /translation="MKYVVLVREPLPDDMLEVATKVASGFRIPVDKAEKLLTRAPGPV
                     TRPVQEREARTVQSIMEAAGLVVEVREEDQDGPLAAFSTSERDRPATGPAVSIDPSST
                     AVLARSESDVPAYSDGSDAAGTDFSGAGAAGAGVGAAGATDVSVVTSGEGRAWRTADR
                     HPDPAPENQDARFGRSTRHDANQDPVQSADQARSTDPALGAAGERSADTVG"
     gene            complement(629..>1600)
                     /locus_tag="PKN52_08965"
     CDS             complement(629..>1600)
                     /locus_tag="PKN52_08965"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HNR00121.1"
                     /translation="TTLNGRLSEGVVANVLQYLALSQATGCLALVHPERRQGRVYLEN
                     GVIVLVEARPLYDLAAMAAMLEWREGRFTFRPGEAPKRKTMSRNADTLLLEASHLVDT
                     GEVARGPSAAADTVLHVAESRDRDESVMLTLAALHLWRTIDGVSSLRQLAATIGKPVE
                     ETVAAAQELLDNGLAGFATLAVADPRFVREVARETIDLMGPVGSIVVEDALMDLGVGP
                     DTLPVSAAGEFLEEVARAIHSSERRAEFLRRATELRRLFSLDQQARGGRSDVDARKGG
                     RGGSGRNGSAHTDVARESDRTDAAARGEPKDRGPARGDSATRPRRKP"
BASE COUNT          208 a          586 c          556 g          250 t
ORIGIN      
        1 ccgaccgtgt cggcgctccg ctcgccggcg gcgccgagcg cgggatccgt actgcgcgcc
       61 tggtccgcgc tctgcacggg atcctggttc gcgtcgtgac gggtagagcg cccgaagcgc
      121 gcgtcttggt tctccggcgc cgggtcgggg tgccgatccg ccgtccgcca ggcgcgcccc
      181 tcgccactgg tcaccacgct cacgtccgtc gcgccggcgg cgccgactcc cgcgccagcg
      241 gctccagcgc cagagaaatc ggtgccggcg gcatccgagc cgtcggagta ggcggggacg
      301 tcggattcgc tcctggcgag gacggcggtg gaggacgggt cgatcgagac ggccggtccg
      361 gtggccggac ggtcgcgctc gctcgtcgag aaagcggcca gcggaccgtc ttggtcctcc
      421 tcgcgtacct cgacaacgag gccggcggcc tccatgatgc tctgcacggt gcgtgcctcg
      481 cgctcctgca cgggccgagt aacgggtccg ggcgcgcggg tgaggagctt ctccgccttg
      541 tcgacgggga tgcggaagcc ggacgcgacc ttcgtcgcca cctctagcat gtcgtccggc
      601 agcggttctc tgacgagcac cacgtacttc atggcttcct cctgggtcgg gtggcagagt
      661 cgccgcgagc cgggccccta tccttgggct cgccgcgagc cgcggcgtcg gtacggtcgc
      721 tttcgcgagc gacgtccgtg tgagcggagc cgttgcgccc cgagccccct cgaccgccct
      781 tgcgggcatc tacgtcgcta cgtcctccgc gtgcctgctg gtcgagtgag aagagcctac
      841 gcaactcggt ggcgcgacgc aagaactccg cgcggcgctc gctcgaatgg atggcgcgcg
      901 cgacctcctc caagaactcg cctgcggcgc tgacaggcaa cgtgtccggg ccgaccccga
      961 gatccatcag ggcgtcctcg accacgatgc tgccgaccgg tcccatcaga tcgatcgtct
     1021 cgcgcgccac ctccctgacg aaccgcgggt ccgcgacggc cagggtggcg aaacccgcga
     1081 ggccgttgtc gagcagctct tgagccgccg cgacggtctc ctccaccggt ttcccgatgg
     1141 tcgcggccaa ctgccgcagg ctgctgacgc cgtcgatggt gcgccacagg tgcagggccg
     1201 ccagcgttag catcacggac tcgtcgcggt cgcggctctc ggcgacgtgc aggaccgtgt
     1261 cggcggcggc gctcgggccg cgcgctacct cgcccgtgtc aaccagatgc gacgcctcga
     1321 gcaggagggt atccgcgttc cgactcatgg tcttccgctt cggcgcctcg cccgggcgga
     1381 acgtgaagcg cccctcgcgc cactcgagca tggccgccat ggccgccagg tcgtagagcg
     1441 gccgcgcctc cacgagcacg atgacgccgt tctcgaggta cacgcgccct tgacggcgct
     1501 cggggtggac gagcgcaagg cagcccgtcg cctggctgag cgccaggtac tgcagcacgt
     1561 tggcgaccac cccttcactc agccggccgt tcaatgtcgt
//
LOCUS       DAOQYE010000565         1599 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_95365_length_1599_cov_3.030440, whole genome shotgun sequence.
ACCESSION   DAOQYE010000565 DAOQYE010000000
VERSION     DAOQYE010000565.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95365_length_1599_cov_3.030440"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="PKN52_08970"
     CDS             complement(<1..266)
                     /locus_tag="PKN52_08970"
                     /inference="COORDINATES: protein motif:HMM:NF026035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00122.1"
                     /translation="MPRYPQTPRRRLLACCLLVLTLGGPALAADYADRFEAAWNLVAT
                     RYWNIARTGVDWVAVGDEYRPRAIAAADDGEFYEVLEEMYAELG"
     gene            complement(235..1167)
                     /locus_tag="PKN52_08975"
     CDS             complement(235..1167)
                     /locus_tag="PKN52_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HNR00123.1"
                     /translation="MAHFFVVGDATVDQMYFVGEFPDPGGEVSAIRAVMQPGGSGGTV
                     ATVLARLGNDTRIATRVGTGPFSDLALRNLTAAGVDMRLVQRDATLQTSSVTLIITPD
                     AQRTMISAAGASRHLDSAELREDAIADCDALVMSAYSLMGGPQKEYALQALNHKLTTF
                     VDLGTGAVRALQDRLVPTLRGIDYWLMNERELYMLTGRSTISEAVRDLRDDGVEQMVV
                     KVGAMGSIVINAEFTELVEAHEIDNVVDSTGAGDYYTAAFAHAVMAGHDLYSAARLGN
                     VAGALNTTVVGAQSLLLDAITLERHAAIPTDATA"
     gene            complement(1290..>1599)
                     /locus_tag="PKN52_08980"
     CDS             complement(1290..>1599)
                     /locus_tag="PKN52_08980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00124.1"
                     /translation="TFAVWISSKSAGSGVAATSAVVLIDTDPAATPDPALFELIHTAF
                     AHRRKTLVKNLVLAGYERGVSEAALADLGLQPLARAEELDLATFKELEAKLRGRAPPV
                     "
BASE COUNT          235 a          520 c          573 g          271 t
ORIGIN      
        1 tcgccgagct ccgcgtacat ctcttcgagg acctcgtaga actcgccgtc atctgctgct
       61 gcgatggctc ttggccggta ctcgtcgcct accgccaccc agtcgacgcc cgtcctcgcg
      121 atgttccagt agcgcgtggc caccaggttc caggccgcct cgaaacgatc ggcgtagtct
      181 gccgcgaggg cggggccgcc gagagtcagg acgagcaggc agcaggctag gagcctacgc
      241 cgtggcgtct gtgggtatcg cggcatggcg ttccagtgtg atggcgtcca gcaggaggct
      301 ctgagcgcca accaccgtgg tattgagcgc accggcgacg ttgccgaggc gcgcggcgct
      361 gtacaggtcg tggccggcca tgacggcgtg agcgaaggcg gccgtgtagt agtcgcccgc
      421 gccggtcgaa tcgacgacgt tgtcgatctc gtgcgcttcg acgagttccg tgaactcggc
      481 gttgatgacg atggagccca tggccccgac cttgaccacc atctgctcga cgccgtcgtc
      541 gcgcaggtcg cgcacggcct ccgagatggt cgagcggccc gtcagcatgt acagctcgcg
      601 ctcgttcatc agccagtagt cgatgccgcg cagcgtcggt accaggcggt cctgcagcgc
      661 cctgacggcc ccggtgccga ggtcgacgaa cgtggtgagc ttgtggttga gcgcctgcag
      721 ggcgtactcc ttctggggac cgcccatgag gctgtaggcg ctcatgacga gggcgtcgca
      781 gtcggcgatg gcgtcctccc ggagttcggc ggagtcgaga tggcggctcg cgccggcggc
      841 gctgatcatg gtgcgctgcg catccggcgt gatgatgagg gtgacggaac tcgtctgcag
      901 cgtggcgtcg cgctggacga ggcgcatgtc gacgccggcg gccgtgaggt tgcgcagggc
      961 gaggtcggag aacgggcccg tcccgacccg cgtggcgatg cgcgtgtcgt tgccgaggcg
     1021 ggcgaggacg gtggccaccg tgcctccgga gccgccaggc tgcatgacgg ccctgatggc
     1081 ggacacctcg ccacccgggt ccgggaactc cccgacgaag tacatctggt cgacggtcgc
     1141 gtcgccgacg acgaagaagt gggccatgcg cagttactct cctaccggcg tgccccggcg
     1201 cggtgccggg agtcgggcgc tttctttgct taggggagta tacccacgtc attcatgagg
     1261 attggtcgtg ccgtggcgcg tgtccgcttt cacaccggcg gcgcccgccc acgcagcttg
     1321 gcctccaact ccttgaacgt cgcaaggtcg agttcctccg cccgagccag cggctgcagg
     1381 ccaaggtcgg cgagggccgc ctcggagacc ccgcgctcgt agccggccag gacgaggttc
     1441 ttgaccaacg tcttgcgccg atgcgcgaac gccgtatgga tcagctcgaa gagcgctggg
     1501 tcgggtgtgg ccgccgggtc ggtgtcgatc agaacgacgg cggacgtggc ggccacaccc
     1561 gacccagcgc tcttcgagct gatccatacg gcgaacgtg
//
LOCUS       DAOQYE010000566         1599 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_95386_length_1599_cov_2.582902, whole genome shotgun sequence.
ACCESSION   DAOQYE010000566 DAOQYE010000000
VERSION     DAOQYE010000566.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95386_length_1599_cov_2.582902"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1307)
                     /gene="ffh"
                     /locus_tag="PKN52_08985"
     CDS             complement(<1..1307)
                     /gene="ffh"
                     /locus_tag="PKN52_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HNR00125.1"
                     /translation="MFDGLRGRGRLTEADVKAALREVRVALLEADVNLEVAREFTNRV
                     QEKAVGSDVLMSLQPDQRVIAIVHDELKEVLGGKSVLPTLKSDGNVWLLMGLQGAGKT
                     TTAGKLAYRYKSQGRRVLLVAADTQRPAARDQLKVLGEQIGVPVFEVNDGEAPASSAA
                     RLKEHLRKDFRDLVIVDTAGRLQIDDALMDELADLSERVGPSERILVVDAMTGQQSLP
                     VATAFDERIGVTGLIMSKLDGDARGGAALSAKHVTGKPIYFAGMSEKIDGLEPFHPDR
                     VAGRILGMGDVLTLIEKAKALEGDDEDDAQMRNLGDFSLQDMLTQMRRIKQMGSFTDV
                     LKLIPGAGKIVPAGTNIDEREIGRVEAIISSMTEAERHTPRILNASRRKRIARGSGTT
                     VQDVNRLINNYEEMRKLMKRMGKRAPRRGGRPGLGGDAEADGHL"
BASE COUNT          226 a          556 c          541 g          276 t
ORIGIN      
        1 aggtgaccgt cagcctcggc gtcgccaccg aggcccggcc tgccgccgcg acggggcgcg
       61 cgcttgccca tgcgtttcat caacttgcgc atctcctcgt agttgttgat gaggcggttc
      121 acgtcctgca cggtggtgcc ggagccgcgg gcgatgcgct tgcggcgcga ggcgttgagg
      181 atgcgcggcg tgtggcgctc ggcctcggtc atggacgaga tgatcgcttc cacgcgaccg
      241 atctcgcgtt cgtcgatgtt cgtgccggcc ggcacgatct tgccggcgcc ggggatgagc
      301 ttcagcacgt cggtgaacga ccccatctgc ttgatgcgcc gcatctgcgt gagcatgtcc
      361 tggagggaga agtcccccag gttgcgcatc tgggcgtcgt cttcgtcgtc tccctcgagg
      421 gccttggcct tctcgatgag ggtgaggacg tcgcccatgc cgaggatgcg cccggccacg
      481 cggtcgggat ggaacggctc gagcccgtcg atcttctcgc tcatgcccgc gaagtagatg
      541 ggcttgcccg tcacgtgctt cgccgagagg gcggcgccgc cgcgagcgtc gccgtcgagt
      601 ttcgacatga tgaggccggt cacgccgatg cgctcgtcga acgccgttgc gaccggcagc
      661 gactgctgac ccgtcatggc gtccacgacg aggatccgct cgctcgggcc cacgcgctcg
      721 ctcaggtcgg ccaactcgtc catcagcgcg tcgtcgatct gcaagcgacc ggccgtgtcg
      781 acgatcacca ggtcgcggaa gtccttgcgc aggtgctctt tgaggcgcgc ggcgctggag
      841 gcgggcgcct cgccgtcgtt cacctcgaag acagggacgc cgatctgctc gccgaggacc
      901 ttgagttggt cgcgagcggc cggccgttgc gtatcggcgg cgaccagcag cacgcgccgc
      961 ccttgcgact tgtagcggta ggcgagcttg ccggccgtcg tggtcttgcc ggcgccctgc
     1021 aggcccatga ggagccagac gttgccgtcg gacttcagcg tcggcaacac cgacttgccg
     1081 ccgaggacct ccttcagctc gtcgtggact atcgcgatca cgcgttgatc gggctgcagc
     1141 gacatgagga cgtcggagcc gacggccttc tcctgcaccc ggttggtgaa ctcgcgcgct
     1201 acctcgaggt tgacatcggc ttcgaggagc gcgacccgca cctcccgcag cgcggccttg
     1261 acgtcggcct cggtgagccg accacggccc cggaggccgt cgaacacgga ttggagtcga
     1321 tcacccaaag actggaacat gctctaagaa gtccttaccc gtaatggtcg gcctacgcta
     1381 gctctcacac gccggtcgcc gcgcctactg cgtaccatgt gcccggcgcc ccgtggtagg
     1441 ccccggacac gacggccgcg gccaccacaa ggccctgaca ggccccgccg cgctcggccc
     1501 gccccggcga gggaggtcga aagcccctgc gcccggtgct agactcgccg catgacggcg
     1561 accgacgtgg ccaacgacgc tgccgctggt ctcttcccg
//
LOCUS       DAOQYE010000567         1599 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_95388_length_1599_cov_2.561528, whole genome shotgun sequence.
ACCESSION   DAOQYE010000567 DAOQYE010000000
VERSION     DAOQYE010000567.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95388_length_1599_cov_2.561528"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1599
                     /locus_tag="PKN52_08990"
     CDS             <1..>1599
                     /locus_tag="PKN52_08990"
                     /inference="COORDINATES: protein motif:HMM:NF018208.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00126.1"
                     /translation="QLEYRRGNLRLAEERLARLGERMADLTGQTARAGEELARIAAAT
                     PPTEPPESPDAAGRNVAAALAAEREAEAAFASAMALEGSKRQEYERLRAEHAEAERRA
                     AALASTRSSLDAQLAEVAEQLTGLASDAGLADLPAAIARQTAAAAELAGVEAQVETHR
                     TTLTKAHEEHALLSAEAVSRTRAAARSRAAFEARRGFAQGPRNALSSGLAGVIGAVAD
                     LVKVAPAYQEAIAGALGRRAEYVVVTDSDAGEAVIAHVRRSGGFVTVLPLDLLRRQAE
                     RSYPGMAGRRGVVGPALDLVTTAPQYQVLFENLLGGTVVVRAMTDATAIARQEAHRPR
                     LVTLEGDIVEATGAMSGGKRSQHATVLGAAAELEEAEAAATAATAASEASAEGLTTAQ
                     AALRSLLDEQRLAAEENRAAAQALAAAREEHAGHERLVADLQARKERLTADAAELAAR
                     DAGTDQAQSAAQLAAAVTAAEAALAEAAGALAAARERVAERKLASSSARQAAMLVEER
                     WRTYLDAEARFAAGAERARVLRDIL"
BASE COUNT          227 a          596 c          610 g          166 t
ORIGIN      
        1 cagctcgagt accggcgcgg caacctgcgg ctcgccgagg agcgcctcgc gcgcctcggc
       61 gagcgtatgg ccgacctgac cggccagacg gcacgggccg gggaggaact cgcccgcata
      121 gcagccgcca cgccgccgac agagccgccg gagtcgccag acgccgccgg ccggaacgtg
      181 gcagccgcgc tggccgccga acgcgaggcc gaggcggcct tcgcctccgc catggcgctc
      241 gaggggtcga agcggcagga gtacgagcgc ctgcgcgcag agcatgcaga agcggagcgc
      301 agggccgccg ccctcgcctc gacgcgcagt tcgctcgacg cccagttggc ggaggtggcg
      361 gagcagctca cgggactcgc gtcggacgcg ggtctggcgg acctgcccgc cgccatcgcg
      421 cgtcagaccg ccgcggcggc ggagctggcc ggcgtggagg cccaggtcga gacccacagg
      481 accacgttga cgaaggcgca cgaggagcac gcgctcctga gcgccgaggc cgtctcccgg
      541 acgcgcgcgg ccgcgcgcag cagggccgcc ttcgaggccc gtcgcggctt cgcgcaaggc
      601 ccgcgcaacg ccctgagttc cggtctggcc ggggtgatcg gcgccgtggc cgacctcgtg
      661 aaggtcgccc cggcgtacca ggaggccatc gcgggggcgc tcgggcgccg ggcggagtac
      721 gtggtcgtaa cggattccga cgccggcgag gccgtcatag cgcacgtgcg gcgctccggc
      781 ggcttcgtca ccgtcttgcc cttggacctc ctccgtcgtc aggccgagcg cagctacccc
      841 ggcatggcag ggcgccgcgg cgtggtcggg cccgcgctgg acctcgtcac gaccgcgccg
      901 cagtaccagg tcctcttcga gaacctcctc ggcgggaccg tggtggtcag ggctatgacg
      961 gacgcaacgg cgatagcgcg ccaggaagcg caccgccccc gcctggtgac gctggaaggc
     1021 gacatagtcg aggccacggg cgcgatgtcg ggcggcaaac gctcgcagca cgccaccgtg
     1081 cttggggcag ccgccgagct cgaggaggcc gaagccgcgg caaccgctgc gacggccgcc
     1141 agcgaagcaa gcgccgaggg cctgacgacc gcccaggccg ccctgcgttc gttgctggac
     1201 gagcagcgcc tggccgccga ggagaaccgc gccgcggccc aggcactcgc cgccgcccgg
     1261 gaggaacacg ccgggcacga acggctcgtg gcggacctcc aggcgcgcaa ggagcggctg
     1321 accgccgatg ccgccgagct ggccgcccga gacgctggca ccgaccaagc gcagtccgcc
     1381 gcccagctcg ccgcggcggt gaccgctgcc gaggccgcgc tggccgaagc cgccggtgcg
     1441 ttggcggcgg ctcgcgagcg cgtggccgag cggaagctag cctctagttc ggcgcgccag
     1501 gcggccatgc tggtcgaaga gcgctggcgc acgtacctcg acgccgaggc gcgcttcgca
     1561 gccggcgccg agcgggctcg cgtgctgcgc gacatcctc
//
LOCUS       DAOQYE010000568         1597 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_95596_length_1597_cov_2.084955, whole genome shotgun sequence.
ACCESSION   DAOQYE010000568 DAOQYE010000000
VERSION     DAOQYE010000568.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95596_length_1597_cov_2.084955"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="PKN52_08995"
     CDS             complement(<1..341)
                     /locus_tag="PKN52_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HNR00127.1"
                     /translation="MAFRDVLDIEVHGGKGGDGGLSFMRLKYIPKGGPDGGHGGKGGS
                     VLLRAVDDITSLDRLVGRTHFKAPVGQQGEGRNKAGLGGGDLYVDVPVGTLATDRDTG
                     DTRQRVWQVQLR"
     gene            complement(439..723)
                     /gene="rpmA"
                     /locus_tag="PKN52_09000"
     CDS             complement(439..723)
                     /gene="rpmA"
                     /locus_tag="PKN52_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HNR00128.1"
                     /translation="MAHKKGVGSTRNGRDSHSQRLGVKRFDGQVVLAGNILVRQRGTR
                     FHPGRNVGVGSDFTLFALRDGVVRFHDKGAKGRFVSIEADSGGAGAVAAD"
     gene            complement(745..1050)
                     /gene="rplU"
                     /locus_tag="PKN52_09005"
     CDS             complement(745..1050)
                     /gene="rplU"
                     /locus_tag="PKN52_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HNR00129.1"
                     /translation="MFAIVEAGGKQYRVAEGDVLQVEKIAAEPGATVELPVLLIGGET
                     VKVGAPHVAGASVTAEVVGHGRGEKIHIYKFKAKANYRRHTGHRQEFTSVRITGIRG"
     gene            1240..>1597
                     /locus_tag="PKN52_09010"
     CDS             1240..>1597
                     /locus_tag="PKN52_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00130.1"
                     /translation="MVDREPSRSVGTEPGLRPDVPELMSPAGHWPQLRAAVEAGADAV
                     YFGLGHFSARSKVGFALDEVPDVMRELHGRAVRGYVTFNTLVFDNELPAARLALEALG
                     AAGVDAVIVQDVAAAAL"
BASE COUNT          229 a          576 c          524 g          268 t
ORIGIN      
        1 cgcaactgca cctgccagac gcgttgccgg gtgtcgcccg tgtcgcggtc cgtggccagc
       61 gtgccgaccg gcacgtcgac gtacaggtcg ccaccgccga ggccggcctt gttgcgacct
      121 tcgccctgct gaccgaccgg cgccttgaag tgcgttcggc cgacgagacg gtcgagcgac
      181 gtgatgtcgt cgaccgcccg cagcagcaca gagccgccct tgccgccgtg tccgccgtcc
      241 ggaccgccct ttggtatgta cttgaggcgc atgaacgaga gcccgccgtc gccgcccttg
      301 ccgccatgca cctcgatgtc taggacgtcc ctgaacgcca ttccagcctc gttccaacaa
      361 gaacgcggcc ccttcgggag ccgcgcctaa agtccatcgt cttgacctag aaggcgcctt
      421 ggaggcgccc ccaagcgctc agtccgccgc tacggcgccc gcgccgcccg agtcggcttc
      481 gatgctgacg aagcggccct tggcgccctt gtcgtggaaa cgcaccacgc cgtcgcgcag
      541 ggcgaagagc gtgaagtcgg acccgacgcc gacgttacgg ccggggtgga agcgcgtgcc
      601 gcgctgcctc accaggatgt tgcccgcgag gacgacctgg ccgtcgaagc gcttgacgcc
      661 gaggcgctgg gagtgggagt cgcgtccgtt gcgggtgctg ccgacgcctt tcttatgtgc
      721 catctagacc ctctcctagt cgcctcagcc gcggatgccc gtgatccgca cgctggtgaa
      781 ctcttgccga tggcccgtgt ggcgacggta gttcgccttg gccttgaact tgtagatgtg
      841 gatcttctca ccgcgcccgt ggccgaccac ctcggccgtg acgctggcac cggccacatg
      901 cggtgcgccg accttgacgg tctcgccgcc gattagcagg acgggcagct cgacggtggc
      961 gccgggctcg gccgcgatct tctccacttg cagcacgtcg ccttcggcca cgcggtactg
     1021 cttgccaccc gcttcaacta ttgcgaacat ctttcgacct ccgggccgtg gcgcgcctcg
     1081 cgacgcttgc cggcccacgc tacctgaacg cttcgcaccg gacgcgccgc cctcggggtg
     1141 gctgaccgat accagaactg acattgtagc cgatgaaccg gccccgtgac aaccgacccc
     1201 agggagaacg gcgcttgctt gctataccat cgctactcga tggtggaccg agagccaagc
     1261 aggagtgtcg gcacggagcc cggcctcagg cccgacgtgc ccgagctgat gagccccgcc
     1321 ggtcactggc cgcaactgcg cgccgccgtg gaggctgggg cggacgccgt gtacttcggc
     1381 cttggccact tcagcgcacg ctccaaggtg gggttcgccc ttgacgaggt gcccgacgtc
     1441 atgcgcgagc tgcacggccg cgccgtgcgc ggttacgtaa ccttcaacac gctcgtgttc
     1501 gacaacgagc tgccggcggc tcggctcgcc ctcgaggcgc tcggggcggc cggggtggac
     1561 gcggtgatcg tccaggacgt cgccgcggcg gcgctcg
//
LOCUS       DAOQYE010000569         1593 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_95923_length_1593_cov_2.940832, whole genome shotgun sequence.
ACCESSION   DAOQYE010000569 DAOQYE010000000
VERSION     DAOQYE010000569.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95923_length_1593_cov_2.940832"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1077
                     /gene="maeA"
                     /locus_tag="PKN52_09015"
     CDS             <1..1077
                     /gene="maeA"
                     /locus_tag="PKN52_09015"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: protein motif:HMM:NF010052.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate-decarboxylating malate
                     dehydrogenase"
                     /protein_id="HNR00131.1"
                     /translation="LDDPLYLGVRHQRLTGQAYADFIERFVSAFKSRFPGAVLQWEDF
                     SKQKAVDVMNRYRDALPSFNDDIQGTGAVVLAGLLASARKTRRPMVDETYVVHGAGAG
                     GIGVARQIVVGLVRQGLSRAAALARVLMIDSAGLVVADRPGLDDYKRELAQDPSRTAG
                     WQVAGSVPSLLETVINAKATGLIGLSGQAGAFDESVVRAVAENSPAPIVFPLSNPTSN
                     SEAVPEDVYRWTNGRAIVATGSPFADVRLDDQTFVVGQGNNAFIFPGVGLGTLVSGAS
                     KVTDNMLTAAAEALADYTDPSRLMTGAVYPSINAINRASRHVAAAVVKQAVEDGVAHE
                     AVSDPLAAVTDAMWEPRYLPIRRS"
     gene            1101..>1593
                     /locus_tag="PKN52_09020"
     CDS             1101..>1593
                     /locus_tag="PKN52_09020"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HNR00132.1"
                     /translation="MRTVVLRVVALALLLLLALVGAIAVRTMQRVPDTVVYFVRDQGT
                     TFTLEGVNRRLGRSTPAERAAAQVTALAAGPTADEITRGLRSNVPPDTAVLGATFSGG
                     TLTVDLSGAFERGGGTAAMLGRLNQLFYTLTQPADVSAVRLLLDGAAVTVFSADGLLV
                     DDPW"
BASE COUNT          237 a          580 c          542 g          234 t
ORIGIN      
        1 ctcgacgacc ccctctacct cggcgtgcgg caccagcgct tgacgggcca ggcgtacgcc
       61 gacttcatag agcgcttcgt gagcgccttc aagagccgct tcccgggcgc cgtcctgcag
      121 tgggaggact tcagcaagca gaaggccgtc gacgtcatga accgctaccg cgacgcgctg
      181 ccgtccttca acgacgacat ccaggggacg ggcgcagtcg tgctggcggg cctcctcgcc
      241 tcggcccgca agacgcggcg accgatggtc gacgagacgt acgtcgtgca cggtgcgggc
      301 gctggcggta tcggcgtggc caggcagatc gtcgtcggcc tcgtgcggca gggcttgagc
      361 cgcgccgcgg ccctggcccg cgtcctcatg atcgactccg ccggcctggt agtcgccgac
      421 aggccggggc tagacgacta caagcgggag ctcgcgcaag acccgtcgcg caccgcaggc
      481 tggcaggtgg ccggctccgt accctcgctc ctagagacgg tgatcaacgc caaggccacc
      541 ggactgatcg gcctctccgg tcaggcgggc gcgttcgacg agtcggtggt acgggccgtc
      601 gcggagaact ccccggcccc catcgtcttc ccgctctcca acccgacatc gaacagcgag
      661 gccgtgccgg aggacgtcta ccgctggacg aacggcaggg cgatagtggc gaccggtagc
      721 ccgttcgcgg acgtcaggct ggacgaccag accttcgtgg tcggccaagg caacaacgcc
      781 ttcatcttcc cgggggtggg gctgggaacg ctcgtgtcgg gggcgagcaa ggtgaccgac
      841 aacatgctca ccgcggcggc cgaggctctc gcggactaca ccgacccctc ccgcctcatg
      901 accggagccg tgtacccgag catcaacgcc atcaaccgcg ccagccgcca cgtggccgcg
      961 gcggtcgtca aacaggctgt cgaggacggc gtggcgcacg aggccgtgag cgaccccctc
     1021 gcggccgtca ccgacgccat gtgggagccg cgctacctcc cgatcaggcg gagctaggcg
     1081 caggcggcgt cgggtaggcc atgcgcaccg tcgtcctgcg cgtcgtcgcg ctagccctcc
     1141 tgctgctgct ggcgctcgtc ggcgcgatag ccgtgcgcac catgcagcgg gtacccgaca
     1201 cggtcgttta cttcgtcagg gatcagggca ccacgttcac cctcgagggc gtcaaccgcc
     1261 gtctgggtag gtcaacgccg gcagagcgcg ccgccgccca ggtgacggcg ctcgccgccg
     1321 ggccgacggc ggacgagatc acgcgcggtc tgcgctccaa cgtgcctccg gacacggctg
     1381 ttctcggcgc cactttctcg ggcgggacct tgacggtcga cctctcgggc gccttcgagc
     1441 gtggcggtgg caccgccgcc atgctcgggc ggctgaacca gctcttctac accctcaccc
     1501 aacccgccga cgtctccgca gtcaggctcc tactcgacgg cgcggcggtg accgtcttct
     1561 cggccgacgg tctgctggtc gacgacccgt ggg
//
LOCUS       DAOQYE010000570         1593 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_95951_length_1593_cov_2.492848, whole genome shotgun sequence.
ACCESSION   DAOQYE010000570 DAOQYE010000000
VERSION     DAOQYE010000570.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95951_length_1593_cov_2.492848"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1593
                     /locus_tag="PKN52_09025"
     CDS             <1..>1593
                     /locus_tag="PKN52_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase"
                     /protein_id="HNR00133.1"
                     /translation="ADTLHGNVLNCFVQDGSPEEVGTDAWALHLASKLALVTKVGGGN
                     GVNLDPIGQKRRFVGATGRLYLTIDRSHADYRKVATGTFLDLVRGEMVTRGYRAGTFV
                     ERDRLPSGARVRLVGDSVDDIWQAAADAVLALLAGNDIVIDLSDLRAEGTPVAGSGGS
                     SSGPSSFAVEVYDNFAVWASLGGATFAGPVATLRYVFAPTLRAIRQGGTRRGAGMATI
                     SITHPDVDDFITAKDLEREREEGDISTFNISVLVSDEFMRTASGELGRGKLWEIAQHA
                     WGTGEPGLIYVDRINAHNPMRGVLGDIKSTNPCGEIPLYPGEPCDLGALNLAAYVATR
                     DVGLTGYDFDRFATDVATCVRFLDNVLDVNRFALEDNREMSMKLRRLGLGVMGLADAL
                     ILMGYGYDSEEGRDAVSAIVGAMRNAAAKASEALAAERGAFPFFEESDLDTPRRNVAI
                     LTVAPTGTTSMLMGVSSGVEPVFAPFIYRKIGTEYHALIHPLFQELLEEHAPHPDYRK
                     GAKWDWDKVVAAVQANHGSVRG"
BASE COUNT          251 a          587 c          511 g          244 t
ORIGIN      
        1 gcgcggacac cctccacggc aacgttctca actgcttcgt tcaggacggc agcccggaag
       61 aggtcggcac cgacgcgtgg gccttgcatc tcgcgagcaa gctcgcccta gtcaccaagg
      121 ttgggggcgg caacggcgtc aacctcgacc ccatcggtca gaagcgtcgt ttcgtcggcg
      181 ccaccggccg gctctacctc accatcgacc gctcgcatgc cgattaccgg aaggtcgcca
      241 ccggcacctt cctcgacctg gtcaggggcg agatggtgac gcgcggctac cgcgccggca
      301 ccttcgtgga gcgcgatcgc ctgccgtccg gtgcgcgagt ccgcctcgtg ggcgactcgg
      361 tcgacgacat ctggcaggcc gcggccgatg cggtcctggc cctgctggcg ggcaacgata
      421 tcgtgatcga cctctccgac ctgcgcgccg agggcacccc ggtcgccggt tccggtggct
      481 cgtccagcgg cccgtcctcg ttcgcggtcg aggtgtacga caacttcgcc gtgtgggcgt
      541 ccctcggggg cgccacgttc gccgggcccg tcgccaccct gcgctacgtg ttcgcgccga
      601 ccttgagggc gatcaggcag ggcggcacgc ggcgcggcgc cggcatggcc accatctcca
      661 tcacccaccc ggatgtcgac gacttcatca cggccaagga cctggaacgc gaacgcgaag
      721 agggcgacat cagcacgttc aacatctccg tgctcgtctc ggacgagttc atgaggaccg
      781 cgagcggcga gctggggcgg ggcaagctct gggagatagc ccagcacgcc tggggcacgg
      841 gtgagcccgg tctcatctac gtcgatcgca tcaacgccca caaccccatg cgcggcgtgt
      901 tgggcgacat caagagcacg aacccgtgcg gcgagattcc cctctacccg ggtgagccgt
      961 gcgacctcgg cgccctcaac ctcgccgcct acgtcgcgac gcgcgacgtc gggctcacgg
     1021 gctacgactt cgaccggttc gccaccgacg tcgccacgtg cgtgcgcttc ctcgacaacg
     1081 tccttgacgt caaccgcttc gcgctcgagg acaaccgcga gatgagcatg aagctcaggc
     1141 ggctggggct cggcgtcatg ggcctcgccg acgcgctcat cctgatgggc tacggctacg
     1201 actcggagga aggccgcgac gcggttagcg ccatcgtcgg cgccatgcgc aacgccgccg
     1261 ccaaggcctc ggaggccctc gccgcggagc ggggcgcctt ccccttcttc gaggagagcg
     1321 acctcgacac gcctcgtcgc aacgtcgcca tcctgaccgt cgccccgacc ggcaccacga
     1381 gcatgctcat gggcgtctcg tcgggcgtcg aacccgtctt cgcgccgttc atctaccgca
     1441 agatcggcac ggagtaccac gccctcatcc accccctctt ccaggaactc ctcgaggagc
     1501 acgcgcccca ccccgactac cgcaagggcg ccaagtggga ttgggacaag gtcgtagcgg
     1561 ccgttcaggc caaccatggc tcggtgcgcg gtc
//
LOCUS       DAOQYE010000571         1590 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_96186_length_1590_cov_4.074919, whole genome shotgun sequence.
ACCESSION   DAOQYE010000571 DAOQYE010000000
VERSION     DAOQYE010000571.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96186_length_1590_cov_4.074919"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..973
                     /locus_tag="PKN52_09030"
     CDS             <1..973
                     /locus_tag="PKN52_09030"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HNR00134.1"
                     /translation="REEIAARPVDQVVIDEIQKVPALLDEIHWLMENGGPSFALCGSS
                     ARKVKRGAANLLGGRAVRYELFGLSAVEIGAEVDLDRLLNHGYLPRAYLSERPTRLLN
                     SYVSDYLREEVLAEALVRDAPVFAEFLTLAALTDTEQVNFSNIAREAGTSGPTVKAYF
                     EILVDTLLGRWLQPYRRRPKRRVSAAAKFYFADVGVVNYLARRGTLSRRSELYGKAFE
                     NWIHHELTTYAAYSESFTELSYWRLSTGAEVDFIVNDMELAVEAKATAKVTDHHLRGM
                     RELARDHAEVRQRVVVSLEDRSRKTGDGILVVPAGEFVAALWRGEYF"
     gene            complement(1073..1378)
                     /locus_tag="PKN52_09035"
     CDS             complement(1073..1378)
                     /locus_tag="PKN52_09035"
                     /inference="COORDINATES: protein motif:HMM:NF018766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HNR00135.1"
                     /translation="MGLVWLLMLPNASYLITELNQSHRQAEDGVPLWFDIILVISLAM
                     SGVINTVLNVLAAHALVAITLYGDAATDFLRAPVLVAVGAVMILIAFGMYLMPCSLR"
BASE COUNT          301 a          505 c          527 g          257 t
ORIGIN      
        1 ccgcgaggag atcgctgcgc ggccggtcga ccaggtggtc atcgacgaga tccagaaggt
       61 acccgccctg ctcgacgaga tccactggct catggagaac ggcggtccga gcttcgcgct
      121 gtgcggctcg agcgctcgga aggtcaagcg gggagcggcc aacctgctgg gcggcagagc
      181 ggtgcggtac gagctgttcg gcctttcggc cgtcgagatc ggtgccgagg tcgacctcga
      241 ccgtctcctc aaccacggct acctccccag ggcgtacttg tccgagcggc ccaccaggct
      301 gctcaactct tacgtctccg actatctccg cgaagaggtg ttggcagaag ctctcgttcg
      361 cgacgccccg gtcttcgcgg agttcctgac tctcgcggcg ctcacggata ccgagcaggt
      421 caacttcagc aacatcgctc gtgaggccgg cacctcgggc ccgaccgtca aggcgtactt
      481 cgagatcctc gtggacaccc tgcttggccg ctggctgcag ccttaccgtc gcaggccgaa
      541 gcgccgggta tcggcggccg ccaagttcta cttcgccgat gtaggggtgg tgaactacct
      601 ggcccggcgc ggcacgctgt cgcgccgttc ggaactctac ggcaaggcgt tcgagaactg
      661 gatccaccac gaactcacta cctacgccgc ctactcggag tcgttcaccg agctcagcta
      721 ctggcgcctg agcacgggcg cagaggtgga cttcatcgtc aacgacatgg agctggcggt
      781 ggaggcgaag gcgacggcca aggtgacaga ccatcacctc cgcggcatgc gcgagctggc
      841 gagggaccat gccgaagttc gccagcgcgt cgtggtgagc ttagaggaca ggagccgcaa
      901 gacgggcgac gggatcctcg tcgtgcccgc cggtgagttc gtcgccgccc tatggcgagg
      961 agagtacttc taagacgagc ccttcttgag cgcgacccct acagcagtgc ctcccgtagc
     1021 gtctggaacg agatcaacat ctcgatgatc ggccaggccg cgatcaggta gatcagcgca
     1081 aggaacacgg cataaggtac atgccgaaag cgatgaggat catgaccgcg ccgacggcta
     1141 cgagcacggg agcccgaagg aagtccgtgg ccgcatcgcc gtagagcgtt atcgcgacga
     1201 gcgcgtgagc ggcgagcacg ttgaggacgg tgttgatgac gcccgacatc gcgagtgaga
     1261 tcaccaggat gatgtcgaac cagagcggca ccccgtcctc cgcctgtcgg tgcgactggt
     1321 tgagctcggt gatgaggtag ctcgcgttgg gcagcatgag cagccagacg aggcccagcg
     1381 cgggtcatcc tagcgtcagc ccgcgggctc tccgagggcc accccgcggg tcgtctgaaa
     1441 gccgagtcgc ggcaccaaaa caatcttgac taaaagactc aggattaggt acgttgcgcc
     1501 agtgacccca ccacccccga accaggcggc ggcccgtggc tagacgcggc tggcaaggct
     1561 ggttcatgaa cgtcttcggg gcggaggacc
//
LOCUS       DAOQYE010000572         1590 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_96220_length_1590_cov_2.693160, whole genome shotgun sequence.
ACCESSION   DAOQYE010000572 DAOQYE010000000
VERSION     DAOQYE010000572.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96220_length_1590_cov_2.693160"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="PKN52_09040"
     CDS             <1..397
                     /locus_tag="PKN52_09040"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNR00136.1"
                     /translation="GINAELKTEDWTTYGADYRAGKFAMYMIGWNADYADPDNFLLTF
                     FGPAAVGRSGWENADVYAALAQARQIADPDERAALYAKVNDIIADQAMAMPVAHNRSL
                     NATRANIHGWVPSPLGFSSVDLHAVTKTE"
     gene            583..>1590
                     /locus_tag="PKN52_09045"
     CDS             583..>1590
                     /locus_tag="PKN52_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HNR00137.1"
                     /translation="MALADELTLLLGADRVDTTAAVLAQHSRGESYAAGVAPDVVVFP
                     RSTDEVSRVLRFATERGVAVTPVAANSSLEGHTVPVAGGISLDLGQLDRILESRPEDL
                     LVVVQPAVTYPRVNDHVRRHGLFFPIDPGAHASIGGMVSTNASGTAAVRYGTTGDYVL
                     ALEVVTPTGEVLRLGNRARKSSSGYDLCALFTGAEGTLGVITEVTLRLVGLPEAAAAA
                     RLPFPDAFSAASFVTRLIQAGVPVARCELIDPVSIRAVNAYSGTSFPEEMTVFLEFHG
                     NPAGIEAEVEIARELAADSGATAFEAATDAGERARLWEARHTMLFAANAAHPGLDSII
                     TD"
BASE COUNT          239 a          606 c          529 g          216 t
ORIGIN      
        1 cggcatcaac gccgaactga agaccgagga ctggacgacc tacggcgccg actaccgcgc
       61 cggcaagttc gcgatgtaca tgatcggctg gaacgccgac tacgccgacc cggacaactt
      121 cctcctgacg ttcttcggcc ctgcggccgt cggccgcagc ggctgggaga acgccgacgt
      181 ctacgccgcg ctggcgcagg cgcgccagat cgccgatccc gacgagcgcg ccgccctcta
      241 cgccaaggtg aacgacatca tcgcggacca ggccatggcc atgcccgtag cgcacaaccg
      301 ctcgctgaac gccacgcgcg cgaacatcca cggctgggtg ccgagccccc tcggcttcag
      361 ctcggtcgac ctgcacgcgg tcaccaagac cgagtagcgg gtcctcgccg gcgccagcgc
      421 gccggccgac atcaagtaac tccaggtggg gcgtccggag agcatcgggc gccccaccgg
      481 tcgtctgggt ccggtggcgc aggggagctc gggcgtgccc tgggcgccca ccgcggcggc
      541 agccctgaca ggcacgacca cggtcgcatt agagtggggc acatggccct cgccgacgaa
      601 ctcacgctcc tactcggcgc cgaccgcgtc gacacgaccg cggcggtgct cgcccaacac
      661 tcccgcggcg agtcatatgc cgcaggcgtc gcgcctgacg tcgtcgtctt cccccggagc
      721 acggacgagg tgagtcgggt gctgcgcttc gcgacagagc gaggagtggc ggtcaccccg
      781 gtggccgcca actccagcct ggaggggcac acggtgcccg tggcgggcgg catctccctc
      841 gacttggggc agctagaccg catccttgag tctcgccccg aggacctgct cgtcgtcgtg
      901 cagccggccg tcacctaccc gcgggtcaac gaccacgtgc ggcgccacgg cctcttcttc
      961 ccgatcgacc cgggcgctca cgcctccatc ggcggcatgg tcagcaccaa cgcgtccggc
     1021 acggccgccg tccggtacgg gaccacgggc gactacgtgc tcgccctcga ggtcgtgacc
     1081 ccaacgggcg aggtgctgcg cctcggcaac cgcgcccgca agtcctcgag cgggtacgac
     1141 ctctgcgcgc tgttcacggg cgccgagggc accttgggcg tcatcacgga agtgacgttg
     1201 cgcctcgtcg ggctgccgga ggccgccgcg gccgccaggc tgccgttccc tgacgccttc
     1261 agcgccgcct ccttcgtcac caggctcatc caggcaggcg tgcccgtcgc gcgttgcgag
     1321 ttgatcgacc cggtcagcat ccgggccgtc aacgcctact ccggcacgag cttccccgag
     1381 gagatgaccg tcttcctcga gttccacggc aatcccgccg gcatcgaggc tgaggtcgag
     1441 atcgcgcggg agctcgccgc cgactccggc gccaccgcgt tcgaggcggc caccgacgcg
     1501 ggcgagcggg cgcggctgtg ggaggcccgc cacacgatgc tgttcgccgc caacgcggcg
     1561 caccccggcc tggacagcat catcacggac
//
LOCUS       DAOQYE010000573         1577 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_97564_length_1577_cov_3.153745, whole genome shotgun sequence.
ACCESSION   DAOQYE010000573 DAOQYE010000000
VERSION     DAOQYE010000573.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97564_length_1577_cov_3.153745"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..821
                     /locus_tag="PKN52_09050"
     CDS             <1..821
                     /locus_tag="PKN52_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177800.1"
                     /note="back-translocating Elongation Factor EF4; binds to
                     the ribosome on the universally-conserved alpha-sarcin
                     loop; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HNR00138.1"
                     /translation="SFEPETSQALGFGFRCGFLGLLHADIVQARLEREFDLSLIATAP
                     GVVYKVVTTDGSAFDVQNPSELPTPDRLESISEPFVALSVFLPEDYVGAAMGLLQEKR
                     GEMKDMVYHGHRVELRYEVPFGEILYDFHDRLKSLSRGYASMDYHFIEYRAGDLVKID
                     VLVNEEKVDALSIIAHRDKAYDVGRRMVDRMAEVIPRQMFSIPVQAAIGGKILARATV
                     KAFRKDVLAKCYGGDITRKKKLLEKQKKGKARMKQIGTVEVPQEAFLAVLSTEE"
     gene            828..>1577
                     /locus_tag="PKN52_09055"
     CDS             828..>1577
                     /locus_tag="PKN52_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00139.1"
                     /translation="MDGAASATGRTEARRRRGAPLKLRTQVALVIALLSALPNVILIV
                     AVLLPEYRRAGGAWLGTGTLPILLWLVAIVALSALVGYFLSGQLLAPLLKTEREVQAL
                     PRTAERLAAARLPRRPDDPPEVAALKGSFNALLSQVEVQEARRDAFTAALMHDLKTPL
                     IATNNLLSVVRDDDSLDRASRVELVGRLGQEMEALIDLVQRLVDAHRLERSSVPLTRR
                     RASLAELAATVVERLAPLLAERGVMASVRGEG"
BASE COUNT          239 a          569 c          532 g          237 t
ORIGIN      
        1 tctcgttcga gccggagacg tcgcaggccc tcggcttcgg cttccgctgc ggcttcctcg
       61 gcctgcttca cgccgacatc gtgcaggcgc ggctcgagcg cgagttcgac ctgtcgctca
      121 tcgcgacggc gcccggcgtc gtctacaagg tcgtgacgac ggacgggtcc gcgttcgacg
      181 tgcagaaccc cagcgaactg cccactccag acaggctcga gtccatctcg gagccgttcg
      241 tcgccctgtc ggtcttcctg ccggaggact acgtcggcgc cgccatgggc ctcctgcagg
      301 agaagcgtgg cgagatgaag gacatggtct accacggcca ccgcgtggag ctgcgctacg
      361 aggtgccgtt cggcgagatc ctctacgact tccacgaccg cctgaaatcg ctctcgcgcg
      421 gctacgcctc catggactac cacttcatcg agtaccgggc cggcgacctc gtcaagatcg
      481 acgtgctcgt caacgaggag aaggtcgacg cgctctcgat catcgcgcac cgcgacaagg
      541 cgtacgacgt tggcaggcgc atggtcgacc gcatggccga ggtcatcccg cgccagatgt
      601 tctccatccc cgtccaggcc gccatcggcg gcaagatcct ggcgcgcgcg accgtgaagg
      661 cgttccgcaa ggacgtgctc gccaagtgct acggcggcga catcacgcgc aagaagaagc
      721 tcctggagaa gcagaagaag ggcaaggccc gcatgaagca gatcggcacg gtcgaggtgc
      781 cgcaggaagc gttcctcgcc gtcctgagca cggaggagtg acaaggggtg gacggggcgg
      841 ctagcgctac gggtcggacc gaggcgcgcc gccggcgcgg cgcccccctc aagctgcgca
      901 cgcaggtcgc cctcgtcatc gcgctgctgt cggcgttgcc gaacgtcatc ctcatagtcg
      961 ccgtgctcct cccggagtat cggcgcgccg gcggcgcctg gttgggcacc ggtacgctgc
     1021 ccatcctcct ctggctcgtc gccatcgtcg ccctctccgc gctggtgggg tacttcctgt
     1081 cgggccaact cctcgcgccc ctcctcaaga ccgagcgcga ggtgcaggcg ctgccgcgga
     1141 cggccgagcg cctcgccgcc gccaggctgc cgcgccgacc ggacgacccg cccgaggtgg
     1201 cggcgctcaa gggctcgttc aacgcgctgc tctcccaggt ggaggtgcag gaggcgcgcc
     1261 gcgacgcgtt cacggcggcc ctcatgcacg acctcaagac cccgctcatc gcgaccaaca
     1321 acctcctcag cgtcgtccgc gacgacgact cgctcgaccg ggcgagccgc gtcgagctcg
     1381 tcggacgcct cggccaggag atggaggcgc tcatcgacct cgtccagagg ctagtcgacg
     1441 cgcataggct ggagcgctcc agcgtgcccc tcacgcgccg gcgggcgagc ctggcggagc
     1501 tcgccgcgac ggtcgtcgag cgcctcgccc cgctcttggc ggagcgcggc gtgatggcct
     1561 ccgtgagggg ggagggc
//
LOCUS       DAOQYE010000574         1577 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_97588_length_1577_cov_2.639290, whole genome shotgun sequence.
ACCESSION   DAOQYE010000574 DAOQYE010000000
VERSION     DAOQYE010000574.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97588_length_1577_cov_2.639290"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            581..1027
                     /locus_tag="PKN52_09060"
     CDS             581..1027
                     /locus_tag="PKN52_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00140.1"
                     /translation="MRRHRIATALASALAATVLTCGAYAHQSIAVLDSAYTVIVGLLV
                     NPAYSGQLNGIDLAVRDAGGQAVTGLEKSLTAVVIAPDGSELTLTLRANSAKEGWYTG
                     NFIPSVQGNYTFRVGGFIGTESFEAYFDEPAHSDPAVLDASTITVP"
     gene            1024..1482
                     /locus_tag="PKN52_09065"
     CDS             1024..1482
                     /locus_tag="PKN52_09065"
                     /inference="COORDINATES: protein motif:HMM:NF016149.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopC"
                     /protein_id="HNR00141.1"
                     /translation="MRRVALVVAGALLAFAQAHAYLETSTPVAGAVLDPAPAELVLEF
                     SEELEVGFSTFKLFRLDHTLDASDADYAAKLNGLAALLVNEVIGAAGPDDRSVAFDLK
                     PAQGRSAQVTLTPEEALAPGSYVLMWRVLSVDTHVLQDYLTFTVLEPAPR"
BASE COUNT          252 a          572 c          494 g          259 t
ORIGIN      
        1 tcgggtgcct cggtccagtt cgtggttctg gctgcggtgc gttcgctcga ggaaaccttg
       61 cccgtgaacc gcccccgacg cggaaagcac gtatgctccc tagtcggaac atggtgacgt
      121 tcacggcacg acaggtaaga gcgctgcggg cgggcgcgac agcgcacttc gtatgcaccc
      181 tggtgttgct gttcctgagc ctgccgagct gggcgtccca tacgaacttc cctggtcacc
      241 accacccgga aggcaccccc gatcacgttc acggtcttca gcaggtaggc cttgtcagca
      301 cggcgattcc cgccgcggcc gccgacacgc tgcctatgcc gtcgcccgca tactcgctgc
      361 ccatggcgga agagccgcgg ctcgccacgt tcctgttcca accggcctcg gcccgagccc
      421 caccgcgccg gacaccacat ggcccgacca gaccccgttc ggtaagggcc gcgcatagac
      481 gtacccagca cacgctgcgt agccgtaccc ggcacacgct cagtctttcg aggcgcccgc
      541 ggaccttcgg cgcccaccac acacgcattg gagccaccat atgagaagac atcggatcgc
      601 caccgcccta gcgagcgccc tcgccgccac ggtcctgacc tgcggcgcct acgctcacca
      661 atcgatcgcc gtcctggaca gtgcctacac ggtcatcgtc ggccttctcg tcaaccccgc
      721 ctacagtggc cagctgaacg gcatcgacct ggcggtcagg gacgccggcg ggcaggccgt
      781 tacggggctc gagaagagcc tgacggccgt ggtcatagcg ccggacggca gcgaactcac
      841 cctcaccttg cgcgccaact cggccaagga gggttggtac acgggcaact tcatcccgtc
      901 cgtccagggg aactacacgt tcagggtggg cggcttcatc ggtaccgaga gcttcgaggc
      961 ctacttcgac gagccggcgc acagcgatcc ggcggtgctt gacgcgtcca ccatcaccgt
     1021 tccatgaggc gcgtcgcgtt ggttgtcgcc ggcgcgctcc tcgccttcgc gcaggcgcac
     1081 gcctacctcg agacgtcgac gcccgtcgcc ggcgcggtgc ttgaccccgc gccggcggag
     1141 ctggtcctcg agttcagcga ggagttggag gtcggcttct cgacgttcaa gctgttcagg
     1201 ctcgatcaca cgctcgacgc atccgacgcg gactacgccg ccaagctcaa cggcttggca
     1261 gccctgctcg tcaacgaggt catcggagcc gcggggccgg acgaccggtc agtcgcgttc
     1321 gacctgaaac ccgctcaggg caggtcggcg caggtgacgc tgacccccga ggaggcgctg
     1381 gctcccggca gttacgtgct catgtggcgc gtgctctctg tcgacacgca cgtgcttcag
     1441 gactacctga ctttcaccgt gctggagccg gcgccccgct agggcgcctg gagaagggga
     1501 cggtcgccgc gagcgaaccg agcgcgcgag gctcttgcgt gaacctcgcg cgctcactcg
     1561 gccgtctcta tgcccgt
//
LOCUS       DAOQYE010000575         1576 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_97652_length_1576_cov_3.362262, whole genome shotgun sequence.
ACCESSION   DAOQYE010000575 DAOQYE010000000
VERSION     DAOQYE010000575.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97652_length_1576_cov_3.362262"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /locus_tag="PKN52_09070"
     CDS             complement(<1..661)
                     /locus_tag="PKN52_09070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00142.1"
                     /translation="MADETRTPAWRRALSGWVPIVVVLAVVVALMYPVALLLGLPLAR
                     TRMDNVANWVRSTTAPRSLAEAPTDPSAGAPAGAELRFGSAEGLTTTRSVLVYGDRPV
                     DATYELLDGTARLTGPVAGPLIQWGEALAGPLDVAGVAGANGDPGGGADVAALGAGHV
                     FALPAGRWEALYLGGRLLEAGADVSVELPDGLLTTFTFSEAVAGPVLVNGQLLLEGEG
                     FE"
     gene            complement(675..1517)
                     /locus_tag="PKN52_09075"
     CDS             complement(675..1517)
                     /locus_tag="PKN52_09075"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNR00143.1"
                     /translation="MPEAPVIIDVQDVSKLFRVGKVEAVALQDASLQIHDGQFVSLIG
                     PSGCGKTTLMRLVADLIEPTSGRITVAGKSPKEARAAREYGYVFQAPALYDWRTVLTN
                     VMLPLEIMGWPKGERKARAEEMLKLVGLEGFHRSYPWQLSGGMQQRVSIARALAFDPK
                     LLLMDEPFGALDEITREAMNFELLRLWRQTKKTIVFVTHSIAEAVFLSSHIVVMTARP
                     GRIREVIEVDLPYPRDHETRESERFFELTTRVREALRLGKEGEGGYTAGAGQSDKSLA
                     KVGY"
BASE COUNT          236 a          542 c          566 g          232 t
ORIGIN      
        1 cctcgaaccc ctcgccttcg aggagcagtt ggccgttcac gaggaccggg ccggcgaccg
       61 cctcggagaa cgtgaaggtc gtcagcaggc cgtcaggcag ctccaccgag acgtcggcgc
      121 cagcctcgag gaggcggccg cccaggtaga gggcctccca ccttccggcg ggcagggcga
      181 acacgtggcc ggcgccaaga gcggcgacgt ccgcgccgcc gccaggatca ccgttggcgc
      241 ctgcgacgcc ggcgacgtcc agggggccgg cgagcgcctc gccccactgg atgagcggtc
      301 cggccacggg gccggtcagg cgcgccgtgc cgtcgagcag ttcgtaggtg gcatcgaccg
      361 gtcggtcgcc gtagacgagc acggagcgtg tcgtcgtgag gccttctgca ctgccgaagc
      421 gcaactctgc acccgccggc gcgccggcgg acgggtcggt cggcgcctcg gcaagcgacc
      481 tgggcgccgt ggtcgagcgc acccagttgg ccacgttgtc catgcgcgta cgcgccaggg
      541 ggagaccgag gagcagagca acagggtaca tgagcgccac gaccacggcg agcacgacca
      601 cgatgggcac ccagccggag agcgctcggc gccaggcggg cgtcctcgtc tcgtccgcca
      661 cggcacgcgc cacttcagta accgaccttg gccaggctct tgtcggactg gcctgcgccg
      721 gcggtgtagc cgccctcgcc ctccttgccg agccgcaacg cctcgcgcac gcgcgtggtc
      781 agctcgaaga agcgctccga ttcgcgcgtc tcgtggtcgc gcgggtaggg caggtcgacc
      841 tcgatgacct ccctgatgcg tccgggccgg gccgtcatca cgacgatgtg cgacgagagg
      901 aacacggcct cggcgatcga gtgggtgacg aacacgatcg tcttcttcgt ctgccgccac
      961 agcctcagga gctcgaagtt catggcctcg cgcgtgatct cgtcgagcgc gccgaacggc
     1021 tcgtccatga ggaggagctt cgggtcgaag gcgagcgccc tggctatcga gacgcgttgc
     1081 tgcatgccgc ccgagagctg ccacggatag gagcggtgga agccctcgag cccgacgagc
     1141 ttgagcatct cctccgcccg cgccttgcgt tcacctttcg gccagcccat gatctcgagc
     1201 gggagcatga cgttggtaag gacggtgcgc cagtcgtata gggcgggcgc ctggaagacg
     1261 tagccgtact cgcgcgcggc gcgcgcctcc ttgggcgact tgcccgccac ggtgatacgg
     1321 ccgctcgtgg gctcgatgag atcggcgacc aggcgcatca gcgtggtctt gccgcagccg
     1381 gacgggccga tgaggctgac gaactggccg tcgtggatct gcaggctggc gtcttgcagc
     1441 gccaccgcct cgaccttgcc gacgcggaag agcttgctga cgtcctggac gtcgatgatc
     1501 acgggggcct ctggcacttg gggtcctagt tggtcggcgc gcgcctgagg agcttgccgc
     1561 gcccggggtc gccaac
//
LOCUS       DAOQYE010000576         1576 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_97660_length_1576_cov_2.946746, whole genome shotgun sequence.
ACCESSION   DAOQYE010000576 DAOQYE010000000
VERSION     DAOQYE010000576.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97660_length_1576_cov_2.946746"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="PKN52_09080"
     CDS             <1..390
                     /locus_tag="PKN52_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HNR00144.1"
                     /translation="FELAQVIRPRVVEILDMVRSDIEQRMGALELLAGNVVITGGASL
                     MPGVEQVANDRFRLPVRIGKPDGVSGLSDVVASPAHATAVGLVRYGMAHGPVLAPVVR
                     RQRSSASEATETENFFGRVRKILKDFF"
     gene            414..1493
                     /gene="ftsZ"
                     /locus_tag="PKN52_09085"
                     /pseudo
     CDS             414..1493
                     /gene="ftsZ"
                     /locus_tag="PKN52_09085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00065.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
BASE COUNT          272 a          564 c          525 g          215 t
ORIGIN      
        1 ttcgagctgg cgcaggtcat ccgtccgagg gtcgtcgaga tcctcgacat ggtccgctcc
       61 gacatcgagc agcgcatggg cgccctcgaa ctcctcgcgg gcaacgtggt gataacgggc
      121 ggcgcctcgc tcatgccggg cgtcgagcag gtggccaacg atcggttccg tctccccgtc
      181 cgcatcggca agccggacgg cgtcagcggc ctctccgacg tggtggccag tccggcccac
      241 gccacggccg tcggcctggt acggtacggg atggcccacg ggcccgtcct cgccccggtg
      301 gtcaggcggc aacgctcgag cgcgagcgag gcgacggaga ccgagaactt cttcggtcgc
      361 gtacgtaaga tcctcaaaga cttcttctaa ggtcggcaac ggaaaggcgg gctgaagcga
      421 tgagcaacgt ggacaacgcg atcatccgag tgatcggact gggcggcggg ggcaacaacg
      481 ctgtcaaccg catgatcgag accggcctca ggggggtcga gttcgtcgcc gcgaacaccg
      541 acgcgcaggt cctcgcgacg tcgctcgccg acgtgcgcat ccagatgggc gaccacctga
      601 ccaagggcct cggcgccggc gccaaccccg aggtcggcga gaaggcggcc ctcgaggacc
      661 gcgaccgcat cgccgaggcg ctgcgcggct ccgacctcgt cttcatcacg gccggcatgg
      721 gcggcggcac cggcacgggc agcgcgcccg tggtcgccga ggtcgcccga gagctgggcg
      781 ccctggccat cgcggtcgtg acctcgccct tccagatgga ggggccgcgc cgcatggcgc
      841 aggcgcagga aggcctcagg cggctggagg acaaggtcga cgcgctcatc gtcgtcgaga
      901 accagcgcct cctctccgcg ctcgaccgca aggtacgcct gcaggacgcc ttccgcgtcg
      961 ccgatcgtgt cctctaccac ggcgtcaggg gcatctccga cgtcatcaac gtgcccggcg
     1021 agatcaacgt cgacttcgcc gacgtcaagg cgctcctcca gggcgccggc accgtgctca
     1081 tgggcatcgg cgccggtcga ggcgagaacc tcgcagagga ggcggccgag agcgccacgc
     1141 actcccccct cctcgcccgc ggcgtcgagg gtgcgcgcaa cctcctcatc aacatcacag
     1201 gctctgagaa cctcaccctc ttcgacgctc acgagatcgt caacaagatc agcgaggcca
     1261 ccgaggtgga cgagcccaac gtgctcttcg gcctgaccta cgacgaggac gccggcgacg
     1321 aggtgcgcgt caccatcatc gcggcgggct tcgaccaggc gcctcagacc cgcatcctcc
     1381 ggccgacgca cctgcgcgcc ggactgggcg ccggggccaa caagacgtac gacccgagca
     1441 acttcgacat cccggcgttc ctcaggtaca accccgacgc gaacgacaac tgaagggcgc
     1501 gccgctcagg taaccagagg ttcgcatgcg tgacgtgaag ttccgcggca ggctgttcac
     1561 gaccgccgtc ttggac
//
LOCUS       DAOQYE010000577         1576 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_97697_length_1576_cov_2.242604, whole genome shotgun sequence.
ACCESSION   DAOQYE010000577 DAOQYE010000000
VERSION     DAOQYE010000577.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97697_length_1576_cov_2.242604"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(47..1051)
                     /locus_tag="PKN52_09090"
     CDS             complement(47..1051)
                     /locus_tag="PKN52_09090"
                     /inference="COORDINATES: protein motif:HMM:NF027593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4921 family protein"
                     /protein_id="HNR00145.1"
                     /translation="MPLFRKDPFGRAWVLLSPERGLEPSAFGSVPPAPERSPLSPGSK
                     DRVGPEIAAVRPPGSHAGDADWRIRAIAHPAQLVSSRPTEVDVGVDLFQQAAAYGHHE
                     IIVEHPDARQRLETMPIDHLTEVLRFYRERIALAASRPGIAHVQLTRNVGRAAGAIYE
                     HPHAQLLAVPVTNRWVEEEVAVAAAHHAAHGRCLFCHVLEREIAARERVVTQNDAYVA
                     IAPFASKTPFETWILPRHHGSSFATVASNTLPALAELLRAVVRAINDALDYPPYNLML
                     HTVVEEGRPDYHWHLELLPRLTNQAGFDWGTGFFVNPTLPEDAVRFLREALAMQEVRA
                     "
     gene            complement(1115..>1576)
                     /locus_tag="PKN52_09095"
     CDS             complement(1115..>1576)
                     /locus_tag="PKN52_09095"
                     /inference="COORDINATES: protein motif:HMM:NF014048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease NucS"
                     /protein_id="HNR00146.1"
                     /translation="PAELVRVSFEAPALAVALRLEENGNFVLMGSEAEMQRALARDPS
                     VIEPGLTVIDLELPTDVGGIDLFARDSLGRLVVVELKRGKANHEAVHQLDRYVQSVRA
                     RYAGDVRGVLAAPAVTAPALNRLGVLGLEYREVTALPGLGDEPAKQPGLFG"
BASE COUNT          240 a          518 c          590 g          228 t
ORIGIN      
        1 ggtgaagggc gctcgctccg ggggaagcgg gcgtcccggc cgtcgctcac gcgcggacct
       61 cctgcatggc gagcgcctcg cggaggaagc gcactgcgtc ctccggcagg gtcgggttaa
      121 cgaagaagcc cgtgccccag tcgaaaccgg cctggttcgt caggcgcggc agcagctcga
      181 ggtgccagtg gtagtcgggg cgcccctcct ccacgaccgt gtgcagcatc aggttgtatg
      241 gcgggtagtc gagcgcgtcg ttgatggccc gcacgacggc gcgcaggagt tcggccagcg
      301 ccggcaacgt gttcgacgcc acggtcgcga acgagctgcc gtggtggcgc gggaggatcc
      361 aggtctcgaa cggcgtcttc gatgcgaacg gggcgatggc gacgtaggcg tcgttctggg
      421 tgacgacacg ctcgcgcgcc gcgatctcgc gctccagcac gtggcagaag aggcagcgcc
      481 catgcgcggc gtggtgcgcg gcggccaccg ccacctcctc ctcgacccag cggttggtga
      541 ctggcacggc gaggagttgc gcgtgggggt gctcgtagat ggcgccggcg gcacggccga
      601 cgttgcgcgt cagctggaca tgcgcgatgc cggggcgtga ggcggcgagg gcgatgcgct
      661 cacggtagaa gcggaggacc tccgtgaggt gatcgatggg catggtctcc aggcgctgcc
      721 tggcatccgg atgctcgacg atgatctcat ggtggccgta agcggcggct tgttggaaca
      781 ggtcgacgcc cacgtcgacc tccgtgggtc gggaactgac cagctgagcg gggtgagcga
      841 tcgccctgat gcgccagtcg gcgtcgccgg catgcgaacc gggcggcctc acggcggcga
      901 tctccggtcc taccctgtct ttgctccccg gcgacagcgg cgagcgctcc ggcgccggcg
      961 gcacgctccc gaacgccgac ggttccagcc cacgctccgg cgagaggagc acccaggcgc
     1021 gaccgaacgg atccttgcgg aataacggca tgtgagaggc agtttagaac gtctggagcg
     1081 ggagcggcgt taccaacttc atgcgctgga cacctcaacc gaagaggccg ggctgcttgg
     1141 cgggctcgtc accgagaccg ggcagcgccg tcacctcgcg gtactcgagc cccaggacgc
     1201 cgaggcggtt cagcgccggg gccgtgacag ccggcgcggc gagcacgccg cgcacgtcgc
     1261 ccgcatagcg cgccctgacg ctctgcacgt agcggtcgag ctgatggacg gcctcgtggt
     1321 tcgccttgcc gcgcttgagc tcgactacga ccaggcgacc gagactgtct cgtgcgaaga
     1381 ggtcgatgcc cccgacgtcc gtcggcaact cgaggtcgat gacggtcagc cccggctcga
     1441 tgacggaagg gtcgcgggcg agcgcgcgct gcatctcggc ctcgctgccc atgaggacga
     1501 agttgccgtt ctcctcgagc cggagcgcga cggccaaggc cggcgcctcg aaggagacgc
     1561 gcaccaactc ggcggg
//
LOCUS       DAOQYE010000578         1574 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_97908_length_1574_cov_2.349572, whole genome shotgun sequence.
ACCESSION   DAOQYE010000578 DAOQYE010000000
VERSION     DAOQYE010000578.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97908_length_1574_cov_2.349572"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            124..1224
                     /locus_tag="PKN52_09100"
     CDS             124..1224
                     /locus_tag="PKN52_09100"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HNR00147.1"
                     /translation="MRGTLLTFLPLAVAAFAVAFCAVLWLVPRVRAFALRIGAVQVGG
                     SAHGGGVRQHDGRVPNIGGIAILAGFLVAMLAGSLVAPQLIDGYRVELLAIALGGALM
                     TLVGFIDDMWEVPPPLRLATQVVAAGILVVNGVRIGFVTDYFGAGTFLFIPETLSVLV
                     TILWVVGFTNAFNFIDGLDGLSSGIAAISSMSLLAVAVQFDDRGGAVLLLAALAGAAL
                     AFLRYNFGPATITMGDSGAYLLGYVLAAVSVLGALKVTAAISVVAPVLVLALPVVNIT
                     QVTLRRLRRGSSPALATNDHIHDMIRVRSGSKRFTVVLLWTATLILGVVGMLLSSTPP
                     VLTLLTLIVTVVAIAAVSLLRLAEVRRAGAHT"
     gene            1221..>1574
                     /locus_tag="PKN52_09105"
     CDS             1221..>1574
                     /locus_tag="PKN52_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HNR00148.1"
                     /translation="MSGAVADGPDRQLSTPGGRPPKVVAAFGTRPEANKMAPVVNALA
                     ATPGLEVCTLVTGQHREQLASSLAAFGLTPDADLDVMTERQTLADLFGRIVPAAATEL
                     ERLGADYVLVHGDTTT"
BASE COUNT          188 a          613 c          500 g          273 t
ORIGIN      
        1 cacggctaca tcgtgccggg cctcggcgac gcgggcgacc gcctgtacgg gacgcgctga
       61 ccgtcggaac gcttcgttca gcacactcat gaggccctta ccgccctcat tagaatggtc
      121 tacgtgcgcg gtaccctgct caccttcctc ccgctagccg tcgcggcttt cgccgtcgcc
      181 ttctgcgccg tcctgtggct cgtcccgcgc gtgcgggcct tcgccctgcg catcggcgcc
      241 gtgcaggtcg gcggcagcgc gcacgggggc ggagtgcgcc agcacgacgg gcgcgtccct
      301 aacatcggcg ggatcgccat cctggccggc ttcctcgtcg ccatgttggc cggcagcctc
      361 gtcgcgcctc agctcatcga cggctaccgc gtcgagctcc tcgccatcgc gctcggcggc
      421 gcgctcatga cgctcgtcgg cttcatcgac gacatgtggg aggtcccgcc gccgctccgg
      481 ctcgccactc aggtcgtcgc ggccggcatc ctcgtggtca acggcgtgcg catcggcttc
      541 gtgaccgact acttcggggc cggcaccttc ctgttcatcc cggagacgct ctccgtcctc
      601 gtgacgatcc tgtgggtggt ggggttcacc aacgccttca acttcatcga cgggctcgac
      661 gggctctcct ccggcatcgc cgccatctcg agcatgagct tgctggccgt cgccgtgcag
      721 ttcgacgacc gtggcggcgc cgtcctgctc ctcgccgccc tcgccggggc cgccctcgcc
      781 ttcctgcgct acaacttcgg tcctgcgacc atcacgatgg gcgactcggg cgcctacctc
      841 ctgggttacg tgctggccgc cgtcagcgtc ctcggcgcgc tcaaggtgac ggcggcgatc
      901 tccgtcgtgg cacctgtcct cgtcctggcc ctgccggtcg tcaacatcac gcaggtaacg
      961 ctgcggcgcc tcaggcgcgg cagctcgccg gcgttggcga ccaacgacca catccacgac
     1021 atgatccgcg tgcgctcggg ctcgaagcgc ttcaccgtcg tgctcctctg gacggcgacg
     1081 ctgatcctcg gcgtggtcgg catgctcctg tcgagcacgc cgcccgtcct taccttgctc
     1141 acccttatcg tcaccgtcgt agccatagcc gccgtctcgc tcctgcgcct ggccgaggtg
     1201 cgccgcgccg gcgcgcatac gtgagcggag ccgtcgccga cggccctgac cgccagctga
     1261 gcaccccggg ggggcggccg ccgaaggtgg tcgccgcctt cggcacccgc cccgaggcca
     1321 acaagatggc gccggtcgtc aacgccctcg ccgcgacgcc tggcctggag gtctgcacgc
     1381 tcgtgacggg ccagcaccgc gagcaactcg ccagctccct cgccgcgttc ggactcaccc
     1441 ccgacgccga cctggacgtc atgaccgagc gccagacgtt ggcggacctg ttcggccgca
     1501 tcgtcccggc ggccgcgacc gaactcgagc ggctcggggc cgactacgtg ctggttcatg
     1561 gcgacacgac gacg
//
LOCUS       DAOQYE010000579         1570 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_98289_length_1570_cov_3.054125, whole genome shotgun sequence.
ACCESSION   DAOQYE010000579 DAOQYE010000000
VERSION     DAOQYE010000579.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98289_length_1570_cov_3.054125"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="PKN52_09110"
     CDS             <1..433
                     /locus_tag="PKN52_09110"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNR00149.1"
                     /translation="LVDYVELRPHLNKPLRVLSGGTKQKTSAVLTFLFHPKVLILDEP
                     SAGLDPVASSRLKDRILRERDRGATVILTSHVMSELEELTDEVLFLLEGTVRYTGTLD
                     AIRAGTGERRLERAIAKLMTGEVRLDDGFRILMSERRGEVA"
     gene            430..1221
                     /locus_tag="PKN52_09115"
     CDS             430..1221
                     /locus_tag="PKN52_09115"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNR00150.1"
                     /translation="MNTTLKVMKYAFFDLMRSRWVLIYTLFFLAATGGLLYFGDDAAQ
                     AALSSLNLVLLIIPLVALMLGMNHYYYTRDFVQLILTQPVSRPSVFLGQYAGVALPLA
                     GAFVLGTGVPFVVYSLTHRLDAGLVVSLLGAGALISLVFTALAFWIGLANEERARALG
                     LALGVWLALTVVYDGLVLYFIVLAQAYPIEGAVMAVSVLNPVDLSRILVLMRLDAAAL
                     MGYTGALFNAFLGSATGTMVALGALLAWVVAPVGFGLLAFRRKNF"
     gene            complement(1293..>1570)
                     /locus_tag="PKN52_09120"
     CDS             complement(1293..>1570)
                     /locus_tag="PKN52_09120"
                     /inference="COORDINATES: protein motif:HMM:NF021621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="host attachment protein"
                     /protein_id="HNR00151.1"
                     /translation="SDKPGRTFDSAGDQRHGISPPTDYHRLEKERFAEGIAKRIETAA
                     LSNEFEAVGLPVTLFFDARGNHVLSHIGEVTEVEFLNYLTDLKRGRL"
BASE COUNT          224 a          550 c          516 g          280 t
ORIGIN      
        1 gctcgtcgac tacgtcgagc tgcggccgca cctcaacaag ccgctgcgcg tgctctccgg
       61 cggcacgaag cagaagacga gcgccgtgct cacgttcctc ttccacccga aggtcctcat
      121 actcgacgag ccgagcgccg gcctagaccc cgtcgcgagc agccgcctga aggaccgcat
      181 ccttcgggag cgcgaccggg gcgccaccgt catcctgact tcccatgtca tgagcgagct
      241 cgaggagctg accgacgagg tcctcttcct gctcgagggc acggtgcgct acacggggac
      301 cctggacgcc atccgggcag gcacgggcga gcggcgcctc gagcgggcca tcgcgaagct
      361 gatgaccggc gaggtgcggt tggacgacgg ctttcgcatc ctgatgtcgg agcggagggg
      421 cgaagtagcg tgaacacgac cttgaaggtg atgaagtacg cgttcttcga cctcatgcgc
      481 agccgctggg tcctgatcta caccctgttc ttcctcgccg ccaccggcgg cctcctctac
      541 ttcggcgacg acgcggccca ggcggcgctc agctcgctca acctcgtgct gctgatcatc
      601 ccgctcgtcg cactcatgct cggcatgaac cactactact acacgcggga cttcgtgcaa
      661 ctgatcctca cccagcccgt ctcgcggccc agcgtcttcc tcggacagta cgcgggtgtc
      721 gcgctgccgc tcgcgggcgc cttcgtgctc gggaccggcg tgccgttcgt cgtgtactcg
      781 ctcacgcacc gcctcgacgc cggcctggtg gtcagcctgc tcggcgccgg cgccctgatc
      841 agcctcgtct tcacggcgct ggcgttctgg atcggcttgg ccaacgagga gcgcgctcgc
      901 gccctcggcc tggcgttggg cgtctggctg gcgctgaccg tcgtctacga cggcctagtc
      961 ctctacttca tcgtgctcgc gcaggcctac ccgatcgagg gcgcggtcat ggccgtgagc
     1021 gtcctcaacc cggtggacct ctcgcgcatc ctggtgctca tgcgcctgga cgcggccgcc
     1081 ctgatgggct acacgggcgc gctcttcaac gccttcctcg gcagtgcgac ggggaccatg
     1141 gtggcgctag gggcgctcct ggcctgggtg gtcgcgcccg ttggcttcgg gctgctggcg
     1201 ttcaggcgca agaacttcta gacggcaaac gaaacgggcc cggcatgggc cggacctggc
     1261 cagagcggcg cccgcagcga gtgcggcagg ccctacaagc gcccgcgctt gaggtcggtc
     1321 aggtagttga ggaactccac ctcggtcacc tcgccgatgt ggctgagcac gtggttcccg
     1381 cgcgcgtcga agaagagcgt gacgggcagg ccgaccgcct cgaactcgtt cgagagcgcg
     1441 gccgtctcga tccttttggc gatgccttcg gcaaaccgtt ccttttcgag ccggtgatag
     1501 tccgtcggcg gcgaaatgcc atgccgctgg tcgccggcac tgtcgaacgt tcgaccgggc
     1561 ttgtcggagc
//
LOCUS       DAOQYE010000580         1570 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_98292_length_1570_cov_2.888449, whole genome shotgun sequence.
ACCESSION   DAOQYE010000580 DAOQYE010000000
VERSION     DAOQYE010000580.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98292_length_1570_cov_2.888449"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="PKN52_09125"
     CDS             complement(<1..421)
                     /locus_tag="PKN52_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HNR00152.1"
                     /translation="MTEEPTAVRVEIYDTTLRDGTQGQDVHFTEHDKIAIAKRLDAFG
                     VDYIEGGWPGSNPKDARFFEEMKGVRLNRAKLTAFGSTRHKDLAPEDDGNLKALLDAE
                     TEVLAIFGKSWTFHVEHALGATLDQNLEMIRSSVEYLR"
     gene            complement(431..>1570)
                     /locus_tag="PKN52_09130"
     CDS             complement(431..>1570)
                     /locus_tag="PKN52_09130"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HNR00153.1"
                     /translation="QDCMRADPQFVYQLVRAAIAAGATTVNIPDTTGYGTPQDYGALI
                     RSIFENVPEARGVTLSTHCHDDLGLATANTLAAVENGVTQVEVAVNGIGERAGNTSLE
                     EVAMALYTRRDHYGHDIGINTRELYRVSRLVSRYTGMLVQPNKAIVGENAFAHEAGIH
                     QDGVIKNKSTYEIMNAELVGREAGVLVMGKHSGRNAFRQRLGELGYTDLSAEQLNELF
                     RQFKELADLKQSVTTDDLRALVENEIVKVAETYRLDHLQFQSGTGAMTPQALVRLGTP
                     AEVLEATATGSGPVDAAYKALASLAPIPFELESYDLKAIGSGTDALGEVTIRATADGR
                     TLYGRAISTDVVHASVLAYVDVINKLAAGVGRAKVEAKAVTMAVP"
BASE COUNT          216 a          516 c          539 g          299 t
ORIGIN      
        1 agcgtaggta ctcgaccgac gacctgatca tctcgaggtt ctgatctagc gtggccccga
       61 gcgcgtgctc gacgtggaac gtccacgact tgccgaagat ggcgaggact tccgtctcgg
      121 cgtcgagcaa ggccttgagg ttgccgtcgt cctcgggggc gaggtccttg tggcgcgtgc
      181 ttccgaacgc ggtcagcttc gcgcggttga gccgcacgcc cttcatctcc tcgaagaagc
      241 gcgcgtcctt ggggttcgaa cctggccagc cgccctcgat gtagtcgaca ccgaacgcgt
      301 ccaggcgctt cgcgatcgcg atcttgtcgt gctccgtgaa gtggacgtcc tgaccctgcg
      361 tcccgtcacg cagggtggtg tcgtagatct ctaccctcac ggctgtcggc tcctcggtca
      421 tgcgctcacc tcaaggcacg gccatggtga cggctttcgc ctccaccttg gcgcgcccca
      481 cgcccgccgc tagcttgttg atgacgtcca cgtaggcgag caccgaggcg tgaacgacgt
      541 cggtggagat ggcgcgccca tacaacgtgc gcccatcggc cgtcgccctg atcgtgacct
      601 caccgagcgc gtctgtgccg ctgccgatgg ccttgaggtc gtagctctcg agctcgaacg
      661 ggatcggggc gagcgaggcc agcgccttgt aggcggcgtc gacgggcccc gagcccgtgg
      721 cggtcgcctc gagcacctcc gccggcgtgc cgagcctcac gagcgcctgc ggggtcatgg
      781 cgcccgtgcc cgactggaac tggaggtggt caagcctgta ggtctcggcg accttcacga
      841 tctcgttctc gacgagcgcc ctgaggtcgt cggtggtgac gctctgcttg aggtcggcga
      901 gctccttgaa ctgcctgaag agctcgttga gctgctccgc ggacaggtcg gtgtagccca
      961 gctcgccgag gcgctgcctg aaggcgttgc ggccggaatg cttgcccatc acgaggacac
     1021 ccgcctcgcg tccgacgagc tcggcgttca tgatctcata cgtgctcttg ttcttgatca
     1081 cgccgtcctg gtggatgccg gcctcgtgcg cgaacgcgtt ctcaccgacg atggccttgt
     1141 tcggctggac gagcatcccc gtgtagcgcg acacgagccg cgagacgcgg tagagctcgc
     1201 gggtgttgat gccgatgtcg tggccgtaat ggtcgcggcg cgtgtagagc gccatggcga
     1261 cctcctcgag cgacgtgttg ccggcccgct ccccgatgcc gttgacggcc acctcgacct
     1321 gcgtgacgcc gttctcgacg gcggcgagcg tgttggcggt cgccagccca aggtcgtcgt
     1381 ggcagtgcgt cgagagcgtc acgcccctgg cctctggcac gttctcgaag atggagcgga
     1441 tgagcgcccc gtagtcctgc ggggtgccgt aaccggtcgt gtcggggatg ttgacggtgg
     1501 tggcgccggc cgcgatggca gcgcgcacga gctggtacac gaactgcggg tcggcgcgca
     1561 tgcagtcctg
//
LOCUS       DAOQYE010000581         1570 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_98297_length_1570_cov_2.853465, whole genome shotgun sequence.
ACCESSION   DAOQYE010000581 DAOQYE010000000
VERSION     DAOQYE010000581.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98297_length_1570_cov_2.853465"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="PKN52_09135"
     CDS             <1..363
                     /locus_tag="PKN52_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00154.1"
                     /translation="LVGENAIFAETGDTVRIFFGNGGPNLTSSFHVIGEIFDNVYVEA
                     GGLVNHDVQTTLVPAGGATVVEFKVDVPGTYNLVDHAIFRAFNKGAVGQLVVTGDHDH
                     VIFTERTDIRPYVPADGQ"
     gene            503..1282
                     /locus_tag="PKN52_09140"
     CDS             503..1282
                     /locus_tag="PKN52_09140"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HNR00155.1"
                     /translation="MTTIASTCGKLLASLLVASVCAFGGRATAANMVAVEGGLVEPLF
                     PIEGEGPTLVATFRIDVLPVTNGEFLAFVEAHPEWRRGNVPQVFGGASYLRGWASDTD
                     LGELDPNAPVTSVTWFAAAAYCEARGSRLPTEAEWEFVANAGFSSELGRNEPGYNEAV
                     LALTANRSPVPGPVGLGAANYYGVKDMHGLVWEWVFDIGASFNTGDSRSDGDRRLQLV
                     CGGGSAGAVDKSDYAAFLRYAFRSGLSGDYSSGGLGFRCAN"
     gene            1292..>1570
                     /locus_tag="PKN52_09145"
     CDS             1292..>1570
                     /locus_tag="PKN52_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00156.1"
                     /translation="MRHDWDSSGTALRAASARVTKLGAALLLAAAALFTACDGKSGQA
                     GATQAASAHADHAAMAQHQETQPQAPDAHADHAVTAQTQGTQAQAPNAH"
BASE COUNT          256 a          579 c          525 g          210 t
ORIGIN      
        1 ctggtcggtg agaacgccat cttcgccgag accggcgaca ccgtccgcat cttcttcggc
       61 aacggcggcc ccaacctcac gagctccttc cacgtgatcg gcgagatctt cgacaacgtc
      121 tacgtggagg cgggcggcct ggtcaaccac gacgtgcaga ccacgctcgt accggccggc
      181 ggcgcgaccg tcgtggagtt caaggtcgac gtccccggca cgtacaacct cgtcgaccac
      241 gccatcttcc gcgccttcaa caagggcgcc gtcgggcagc tcgtggtgac cggcgatcac
      301 gaccacgtga tcttcaccga gcgcaccgat atccgccctt acgtcccggc ggacggccag
      361 tagggcttcc actcgagcgc cgggtgacac ggccagcggc cggacccggc gctcccatcc
      421 cgccaggcgc ggcaggcgcc agcgggttac caggagcggc gcacccacgg gcgcgccgcc
      481 acgacctcac cggagaagcg agatgaccac aatcgccagc acctgcggca agctcttggc
      541 atcgctgctc gtcgcctccg tatgcgcgtt cggcggcagg gcgacggcgg cgaacatggt
      601 ggccgtcgag ggcggcctcg tcgagcccct cttcccgatc gagggcgaag ggccgacgct
      661 cgttgccacc ttccggatcg acgtcctgcc agtcaccaac ggcgagttcc tcgccttcgt
      721 cgaggctcac ccggaatggc ggcggggcaa cgtgcctcag gtgttcggcg gcgcctccta
      781 cctgcgcggc tgggcgtcag ataccgacct cggtgagctc gatccgaacg ccccggtcac
      841 gagcgtgacg tggttcgccg cggcggcgta ctgcgaggcg agaggctcgc gcctcccgac
      901 cgaggccgag tgggagttcg tcgcgaacgc cggcttctcc agcgagctcg gccgcaacga
      961 accgggctac aacgaggcgg tcctggcgct gacggccaac cgcagccccg tccccggccc
     1021 cgtcgggctc ggcgcggcca actactacgg cgtcaaggac atgcacggcc tcgtctggga
     1081 gtgggtcttc gacatcggcg ccagcttcaa caccggcgac agccgcagcg acggcgaccg
     1141 gcggctgcag ttagtctgcg gcgggggcag cgccggggcc gtggacaaga gcgactacgc
     1201 cgctttccta cgctacgctt tccgtagcgg cctgagcggc gactactcga gcggcggact
     1261 gggcttccgc tgcgccaact gaggaggcgg catgaggcac gactgggata gctcgggtac
     1321 cgcgctgcgc gctgccagcg cgcgcgtgac gaagctcggc gcggccctcc tgctcgcggc
     1381 cgcagccctc ttcaccgctt gcgacggcaa gagcgggcag gctggggcca cgcaagcggc
     1441 ctccgcccac gccgatcacg ccgccatggc gcagcaccag gaaacgcaac cccaagcacc
     1501 cgacgcgcat gccgaccatg ccgtaacggc gcagactcaa gggacgcaag cgcaagcgcc
     1561 gaacgcgcac
//
LOCUS       DAOQYE010000582         1568 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_98536_length_1568_cov_2.414408, whole genome shotgun sequence.
ACCESSION   DAOQYE010000582 DAOQYE010000000
VERSION     DAOQYE010000582.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98536_length_1568_cov_2.414408"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            171..515
                     /locus_tag="PKN52_09150"
     CDS             171..515
                     /locus_tag="PKN52_09150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00157.1"
                     /translation="MKLTSKKVALGLCSLLLVLSATACRNEAQNRIRRSIQEYTAQRM
                     YITVYSYDGKAVFTGLVDGKVTRSGNASEGGVGDESLGAYIYWYDERGRYHQTDLPYL
                     LTSYERNVPSEP"
     gene            610..1116
                     /locus_tag="PKN52_09155"
     CDS             610..1116
                     /locus_tag="PKN52_09155"
                     /inference="COORDINATES: protein motif:HMM:NF025684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HNR00158.1"
                     /translation="MRTKTLRLAFVALAIALLASACTIYVRPGGFVSDEISLSGVIQD
                     FKPTRGSGSTYYAGENVEFRIVTSQSGYVTLTALDPDGRIYVFERNVPVSAGTTYLPV
                     AGARHVYTVNPPRGLQRVRASFTSTATDGSVRYVGRYGDGEWSNAISVELRPAPVRDV
                     AETNFYIR"
     gene            1341..>1568
                     /locus_tag="PKN52_09160"
     CDS             1341..>1568
                     /locus_tag="PKN52_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNR00159.1"
                     /translation="MAGHSKWAQIKRKKGANDKQRGKVISKHVRAIQSAVRSGNSGDA
                     TTNLALKNALSAAKSDNVPIDNIDRAIERALG"
BASE COUNT          275 a          549 c          503 g          241 t
ORIGIN      
        1 aagcgctaaa gtctgggttg cgagcgaggc gatgctatcc tggcgctggc caggtgcctt
       61 gggacgccgt catgctgtcg gccgtctccg gcccgaccgt cgcgctcgtg ctgccgcgcc
      121 tccgcggccg ttctcgtgcg gcgcccctag gccaggaagg gcggtgaggc gtgaagctca
      181 cctcgaagaa ggtcgcgctg ggcttgtgca gcttgctact ggtgctctcc gccacggcat
      241 gccgcaacga agcccagaac cggatccggc gcagcatcca ggagtacacc gcccagcgga
      301 tgtacataac ggtctactca tacgacggca aggcggtgtt caccggcttg gtcgacggta
      361 aggtgacgcg ctccggcaac gcaagcgagg gcggggtggg cgacgagtcg ctcggcgctt
      421 acatctactg gtacgacgag cggggccgct accaccagac cgacctccct tacctgctca
      481 cctcctacga gcgcaacgtc ccgagcgaac cgtgaaaccc tgaatgaccg aagggacggt
      541 taaggccggc cgaccccttt cgacgccggt gcgttgctac actcgccgcg tgaccaccag
      601 gaggcccaca tgagaaccaa gacgcttcgc ctagccttcg tggcgctcgc catcgccctg
      661 ctcgcctccg cctgcacgat ctacgtgcgg cctggcgggt tcgtgagcga cgagatctcc
      721 ttgagcggcg tcatccagga cttcaagccg acccgcgggt ccggctccac ctactacgcc
      781 ggcgagaacg tcgagttccg catcgtgacc agccagagcg gttacgtgac cctcacggct
      841 ctcgaccccg acgggcggat ctacgtcttc gagcgcaacg tccccgtctc ggccggcacc
      901 acgtacctgc cggtcgccgg ggcccgccac gtgtatacgg tgaacccgcc ccgcggcctg
      961 cagcgcgtgc gtgccagctt cacgtcaacg gccaccgacg gcagcgtgcg ctacgtcggg
     1021 cgttacggcg acggcgagtg gtcgaacgcc atctccgtag agctccggcc cgcgcccgtg
     1081 cgcgacgtcg cggagaccaa cttctacatc cgctgaggcc ctggcggcgc agggcgtacg
     1141 ccacgccctt taccgttcgt caaccacggc ccgcgcgaag cgggccgtga cgcttgagct
     1201 atggatgtac aatgagaacg ccgcggcagc cccctgccgc ggcgcgtgcg agagccgtgg
     1261 accggcttcg gcgcgcctct ggcacgcagg cagcggcgct tggtgtcggg gacgccgcgc
     1321 cttggaaagg aactaccgtc ttggcaggac atagcaagtg ggcccagatc aagcgcaaga
     1381 agggtgccaa cgacaagcag cgtggcaagg tcatcagtaa gcatgtccgg gccatccagt
     1441 ctgccgtgcg aagcggcaac agtggcgacg cgaccaccaa cctcgccctc aagaacgctc
     1501 tgtctgccgc caagtccgac aacgtgccca tcgacaacat cgaccgcgct atcgaacggg
     1561 cgctgggt
//
LOCUS       DAOQYE010000583         1568 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_98541_length_1568_cov_2.366821, whole genome shotgun sequence.
ACCESSION   DAOQYE010000583 DAOQYE010000000
VERSION     DAOQYE010000583.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98541_length_1568_cov_2.366821"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..733)
                     /locus_tag="PKN52_09165"
     CDS             complement(<1..733)
                     /locus_tag="PKN52_09165"
                     /inference="COORDINATES: protein motif:HMM:NF015210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HNR00160.1"
                     /translation="MSFQTPLSIQEMLASIHERKYLMPAIQREFVWGADQIIKLVDSL
                     MRGYPVGSFHLWDVRPETAKAYTFYEFLTDYHERSNPYVDKATVPAGSGTTAVLDGQQ
                     RLTALNIALYGSYAEKKKYAWWNSVDAFPVKRLYLNLLDEPADVELGTKHDLRFLTHA
                     EAAATEGEPDKWFPIGKVLQLADSGPAIMQELKRRGIVESADAAFQRLYDLYRAVRDL
                     RPMNYFLVTDQDADKVLEIFVRVNSG"
     gene            complement(1161..>1568)
                     /locus_tag="PKN52_09170"
     CDS             complement(1161..>1568)
                     /locus_tag="PKN52_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00161.1"
                     /translation="APENPADADWLLVFLEGHTKGQAPWSTRLACLAATDIRDLVQEL
                     EARLAGLEPAVRFHPLEPHLDLAVTPKGDGRFEVEVQVGWRALPAESEPPAGGYLTLK
                     STASSGDLEQLVGRARAILDRYPVRDDLTATNA"
BASE COUNT          255 a          486 c          517 g          310 t
ORIGIN      
        1 caccgctgtt cacgcgcacg aagatctcga gtaccttgtc ggcgtcctgg tcggtgacca
       61 ggaagtagtt catgggtctg agatcgcgta cggcgcgata gagatcgtag aggcgttgga
      121 aggccgcgtc ggcggactcg acgatcccgc gccgcttcag ctcctgcatg atcgcggggc
      181 cggagtccgc cagctgcagc actttgccga tggggaacca cttgtcgggc tcaccttcgg
      241 tggccgccgc ttcggcgtgc gtcaggaacc gaagatcgtg cttggttcct agttccacgt
      301 cagccggctc gtcaaggagg ttcaggtaca ggcgcttgac cggaaaggcg tccacgctgt
      361 tccaccaggc gtacttcttc ttctcggcgt agctgccgta cagcgcgatg ttgagggcgg
      421 tgaggcgttg ctgaccgtcg aggacggcgg tggtcccgct gccggctgga accgtcgcct
      481 tgtcgacata cgggttggag cgctcgtggt agtccgtcag gaactcgtag aacgtgtacg
      541 ccttcgccgt ctcaggcctc acgtcccata agtggaacga gccgaccggg taaccgcgca
      601 tgaggctgtc gaccaacttg atgatctgat cggcgcccca cacgaactcg cgctggatgg
      661 cgggcatgag gtacttgcgc tcgtggatgg acgcgagcat ctcctggatg ctcaacgggg
      721 tctggaagga catctgaacg ctcctaccgg tgcaaacttg tatgcctcag cctagtgaaa
      781 cccggtgggc aggtctgtcg aattcgcttc aaggcgccaa gcggttcatt ccgtatccgc
      841 gtcatcatcg ccggccacac ctcgggccta ccagacttcc tcggccgggc cccagccgcc
      901 caaggacgtg tcgcccagat cgccatggac ttactcgaag tcctcatcgt catcgtccgc
      961 gctgtcgccg aacgagaaca tcggtatccc ggaggcacgt agggtcctca tgtggtccgc
     1021 gtacgacggc gcgtagctcg aggtggcgtc ttcgtcgccc gaggtctcgt gggtagaggt
     1081 ctcgtcggcc gatgcttcgt cagcggccgt tcgcgtggtt ctggagtcgg cttcttcttc
     1141 acgcattcgg ttcctcgctt tcacgcgttg gtggcggtca agtcgtcgcg caccgggtac
     1201 cggtcgagga tggcccgcgc gcggccgacg agttgctcga ggtcaccgct tgaggcagtg
     1261 gacttgaggg tcaggtagcc gccagccgga ggctcggact cggcagggag agcgcgccag
     1321 ccgacctgca cctccacctc gaaccgcccg tcacccttgg gggtaaccgc caggtccagg
     1381 tgtggttcaa gggggtggaa acgcaccgca ggctcaagcc ctgctaggcg agcttcgagt
     1441 tcctgaacca ggtcccttat gtcggtggcc gcgaggcatg ccaagcgcgt gctccaagga
     1501 gcctgcccct tggtgtggcc ttccaagaag acgaggagcc aatcggcgtc cgcgggattc
     1561 tcgggtgc
//
LOCUS       DAOQYE010000584         1568 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_98548_length_1568_cov_2.262393, whole genome shotgun sequence.
ACCESSION   DAOQYE010000584 DAOQYE010000000
VERSION     DAOQYE010000584.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98548_length_1568_cov_2.262393"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            66..527
                     /locus_tag="PKN52_09175"
     CDS             66..527
                     /locus_tag="PKN52_09175"
                     /inference="COORDINATES: protein motif:HMM:NF020133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00162.1"
                     /translation="MTIARALGSDVPFLTGASNAALARGRGERLAALDAPRLHLVLAN
                     PGGQVSAADAYANLVGFTPRLRFAQAMENLAKGVEPGWPNALQPGVLRLLPGVRPVLG
                     ALREAGLLGALLSGSGPTCFGVARDEEHARSVAAALAAQQPAWWVRADSTA"
     gene            complement(622..1008)
                     /locus_tag="PKN52_09180"
     CDS             complement(622..1008)
                     /locus_tag="PKN52_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HNR00163.1"
                     /translation="MTLEEFTSLVAEMVDEIPEEFMRGLGGVHVLPEARQEPEFDDVY
                     RMGEYLDPGTPDFLGAGEGLGRQVVLYYGSFAALAEDDPDFDWEDEAWETLTHELRHH
                     VESLAGEDWLVQEDIEDAGAFPRRRD"
     gene            complement(1022..>1568)
                     /locus_tag="PKN52_09185"
     CDS             complement(1022..>1568)
                     /locus_tag="PKN52_09185"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNR00164.1"
                     /translation="DWGGKGVRPSFTTRASAGGGTLLDNGCHLADLARFFGGSVSEVF
                     ARIGTLKYPVEVEDTAHVSMRYADGSLGVIEAAWTATGWEEGFWVYGTDGALECTNRY
                     GPQNLRHAYRASPGSDWDRTDVTTFEFTGASSHTRHVMAFVAAVRGEGPVVCSGVDGR
                     EAVRLILAAYDSAAANAPVAL"
BASE COUNT          222 a          573 c          552 g          221 t
ORIGIN      
        1 ctccaacgag accaaccgga ctgacacatc atccagccac accggccggc gcgggcgtcg
       61 acaccatgac catcgctcgc gccctcggtt ccgacgtccc gttcctgacg ggcgcgtcca
      121 acgccgccct cgcccggggg agaggcgagc ggttggcggc gctggacgcg cctcgcctgc
      181 acctggtgct cgccaacccg ggcggccagg tcagcgccgc cgacgcgtac gccaacctcg
      241 tgggcttcac ccctcgtctg cgcttcgcgc aggccatgga gaacttggcc aagggggtag
      301 agccgggctg gcccaacgcc ctgcagccgg gcgtgctccg cctcctgcca ggcgtcaggc
      361 cggtcctagg cgcgctccgg gaggccggcc tgctcggcgc gttgctctcc gggagcggcc
      421 cgacatgctt cggcgtggcg cgagacgaag agcacgccag gagcgtggca gccgccctag
      481 ccgcgcagca gccggcctgg tgggtgcggg cggacagcac cgcttagcga gcaggaccgc
      541 ctggccaggc gggcggtcaa ggagcgcgcc gcgcgccgcc ctcgccccac tcgggcctgg
      601 tagcagactg ggcttcccgc ctcagtcgcg cctccgcggg aaagcgccgg cgtcctcgat
      661 gtcttcctgc acgagccagt cctcgccggc cagcgattcg acgtggtgcc gcagctcgtg
      721 cgtcagggtc tcccaggcct cgtcttccca atcgaagtct gggtcgtcct cggcgagcgc
      781 cgcgaacgag ccgtagtaga ggacgacctg gcgaccgagc ccctcgccgg cgccgaggaa
      841 gtcgggcgtg ccagggtcga ggtactcgcc catgcggtag acgtcgtcga actcgggctc
      901 ctgccgggct tccggcagca cgtgcacgcc gccgaggccg cgcatgaact cctccgggat
      961 ctcgtcgacc atctcggcga cgagggaagt gaactcctct agcgtcacgg cagcttccgc
     1021 cctacagggc taccggcgcg ttggcggcgg cgctatcgta ggccgccagg atgagcctga
     1081 cggcttcgcg gccgtcgacg ccgctgcaga cgacgggccc ctctccgcgg acggccgcga
     1141 cgaacgccat gacgtgcctg gtatgtgagg acgcgcccgt gaactcgaac gtggtcacgt
     1201 ccgttctgtc ccaatccgag cctggcgaag cgcggtaggc gtgccggagg ttctggggcc
     1261 cgtaacggtt ggtgcactcc agcgccccgt cggtgccgta tacccagaag ccctcctccc
     1321 agccggtggc cgtccaggcc gcctcgatga cgccgagcga gccgtcggcg tagcgcatgc
     1381 tcacatgcgc cgtgtcctcg acctcgacgg ggtacttgag ggtgccgatg cgagcgaaga
     1441 cctcgctgac ggagccgccg aagaacctgg ccaggtcggc gaggtggcag ccgttgtcga
     1501 ggagggtgcc gccgccggcc gaggcgcgcg tggtgaacga ggggcgcacg cccttgccgc
     1561 cccaatcg
//
LOCUS       DAOQYE010000585         1568 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_98556_length_1568_cov_2.169861, whole genome shotgun sequence.
ACCESSION   DAOQYE010000585 DAOQYE010000000
VERSION     DAOQYE010000585.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98556_length_1568_cov_2.169861"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1387)
                     /locus_tag="PKN52_09190"
     CDS             complement(<1..1387)
                     /locus_tag="PKN52_09190"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNR00165.1"
                     /translation="MRRATRLHYLSIIALLVVVVGAIVWLLATAPDAVVRNRMLVVPG
                     AWQRFLAAFVLAVGGVAGTAYLYGLATRPMRASRYAVTAGTHVFIWLVIGAVFYPVVY
                     LLSVSLNRNDTLAGSLPTAGNLLVRAGVLPDPADFSTSQFEKILSQTHVYGYQWLLVA
                     LMLLAVVVLVVGFVLPRFGVTGARAERVRSYAGWTIFVSAAALILSVQPGQFYGIRAS
                     DGARLPASIGGMVPLYIRNTLLVSGVTGVMAVLLSTTAGYAFSRLRFQGRYGTLLAFV
                     FVQMFPTFMALVAIFYLMSRLDLLNTFTGLILAYSGGAIAFSSWIFKGYLDSLSPSLE
                     EAAMVDGATRWGSFWRVILPISVPMLLFIFLQQFIGTYSEFILANTILVGQDLWTVGV
                     GLRNFSTNQFATQWGAMAAAAVLGSIPILVIFFAVMALLEDVGYMARAAFVMDRFMHL
                     VGLHGKSFIPMC"
     gene            complement(1384..>1568)
                     /locus_tag="PKN52_09195"
     CDS             complement(1384..>1568)
                     /locus_tag="PKN52_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNR00166.1"
                     /translation="TARGTDILISWAYNEAFRSQGGYAYGFGSAISLLIFVITLAVSL
                     MNFRVTGALKEEGARA"
BASE COUNT          315 a          487 c          556 g          210 t
ORIGIN      
        1 ggcacatggg gatgaagctc ttgccgtgca gacccaccag gtgcatgaag cggtccatga
       61 cgaaggccgc ccgggccata taccctacgt cttcgagaag cgccatcacg gcgaagaaga
      121 tgacgaggat cgggatgctg ccgagcacgg ccgccgctgc catcgcgccc cactgcgtgg
      181 cgaactggtt ggtcgagaag ttcctcaggc cgacgcccac cgtccagagg tcctggccga
      241 cgaggatcgt gttggcgagg atgaactcgc tgtacgtgcc gatgaactgt tgcaggaaga
      301 tgaacagcag catcgggacg gagatcggta gtatcacgcg ccagaacgag ccccagcgcg
      361 tcgcgccgtc gaccatcgcc gcctcctcca ggctgggact gagcgagtcg aggtagccct
      421 tgaagatcca ggacgagaac gcgatggcgc cgccggagta agccaggatg agaccggtga
      481 aggtgttcag caggtccagc ctgctcatca ggtagaagat cgcgacgagc gccatgaacg
      541 tcgggaacat ctgcacgaac acgaaggcga gcagcgtgcc gtaccgcccc tggaagcgca
      601 ggcgggagaa ggcgtagccg gcggtggtcg agagcaggac ggccatcacg ccggtgaccc
      661 cggacacgag cagcgtgttg cggatatata gcggcaccat gccgccgatg ctggccggta
      721 gcctggcgcc gtcgctcgcg cggataccgt agaactgccc cggctggacc gacaggatca
      781 gcgcggcggc gctcacgaag atcgtccagc ccgcgtagct cctcacccgc tcagcgcggg
      841 cgccggtcac gccgaagcgc ggcaggacga aaccgaccac gaggaccacg acggccagaa
      901 gcatgagcgc gacgagcagc cactggtagc cgtacacgtg ggtctgggag aggatcttct
      961 cgaactgcga cgtcgagaag tcggctgggt cgggcagtac gccggcgcgc acgagcaggt
     1021 tgccggcggt cggcaaggag cccgccaggg tgtcgttgcg gttgagcgag acggagagca
     1081 ggtagacgac ggggtagaac acggcgccga tgacgagcca gatgaacacg tgggtgccgg
     1141 ccgtgacggc gtagcgcgag gcccgcatcg gtcgggtcgc gaggccgtac aggtacgccg
     1201 tgccggccac gcctccgacg gcgagtacga aggcggcgag gaagcgctgc caggcacccg
     1261 gcaccacgag catgcggttc ctgaccacgg cgtcgggcgc tgtcgccaag agccagacga
     1321 tggcgccgac cacgacgacc aggagggcga tgatgctcaa gtagtggagc cgcgtcgccc
     1381 gcctcatgcc ctggccccct cttccttgag cgcgcccgtc accctgaagt tcatcaggct
     1441 gacggccagg gttatcacga agatgagcag cgagatggcc gagccgaagc cgtaggcata
     1501 gccgccctgg cttcggaagg cctcgttgta cgcccaactg atgaggatgt cggtgcctct
     1561 ggcggtcg
//
LOCUS       DAOQYE010000586         1566 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_98699_length_1566_cov_3.628061, whole genome shotgun sequence.
ACCESSION   DAOQYE010000586 DAOQYE010000000
VERSION     DAOQYE010000586.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98699_length_1566_cov_3.628061"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1081)
                     /locus_tag="PKN52_09200"
     CDS             complement(<1..1081)
                     /locus_tag="PKN52_09200"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNR00167.1"
                     /translation="MPDTHVSAHATAAPRGAGGAQDARSVPALLRLRAADPGTALRVK
                     RLGLWERISWTEYAGRVNAIAREMIHLGLEQGERVAILCENRPEWLIADIAIQTAGGA
                     TVGVYTTSSPEQLAYHVNHSDAVGLILEDAEQLEKWLAVREKCPSVRWVLAVEPEEVD
                     GIVAWEAALTSGAERYASDPGPVEARLAGIGPDDVALFIYTSGTTGNPKGAMLSHANL
                     LWAIDSLADAVPMTERDELLSFLPLSHIVERLISVTAPLRFGYTVSFTENLDTVLTNL
                     QEIRPTVFFAVPRIWEKLHSLVELHMKDANPIKRVAYGAALGAARGGNPLGVALAQLA
                     VLRLLRLRLGLDRVRIAISGAAPIAP"
     gene            complement(1218..>1566)
                     /locus_tag="PKN52_09205"
     CDS             complement(1218..>1566)
                     /locus_tag="PKN52_09205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00168.1"
                     /translation="LDLTGKLVALFGTGDARGYPDTYLDALDAIAKAATAAGATLLGE
                     WSTAGYRFTGSLALRGDSFVGLALDQDNDHDLTEARIGVWCAQLAIEAARALGWPEAA
                     RALGRPEPALAPG"
BASE COUNT          214 a          551 c          580 g          221 t
ORIGIN      
        1 ccggcgcgat cggcgcggcg cccgagatgg cgatgcgcac gcggtcgagg ccgagcctca
       61 gcctcagtag ccgcagcacg gcgagctgcg cgagcgccac ccccagcggg ttgcccccgc
      121 gcgcggcccc gagcgccgcg ccgtaggcca cgcgcttgat cgggttcgcg tccttcatgt
      181 gcagctccac gagcgagtgg agcttctccc agatgcgcgg cacggcgaag aagacggtcg
      241 ggcggatctc ctgcaggttc gtgagcaccg tgtcgaggtt ctccgtgaac gacaccgtgt
      301 aaccgaagcg taggggcgcc gtgacgctga tgaggcgctc gacgatgtgc gagaggggca
      361 ggaaggacag gagctcgtcg cgctctgtca tgggcacggc gtcggccagc gagtcgatgg
      421 cccagaggag gttggcgtgc gacaacatgg cgcccttggg gttgccggtg gtgccggacg
      481 tgtagatgaa gagggctacg tcgtcagggc cgatgcccgc caggcgcgcc tcgaccggac
      541 cggggtcgga ggcgtagcgc tcggcgcctg aggtcagggc cgcttcccag gcgacgatgc
      601 cgtccacttc ctcgggttcg accgcgagca cccagcgcac gctcgggcac ttctcccgca
      661 cggccagcca cttctcgagc tgctccgcgt cctcgaggat gagcccgacc gcgtcggagt
      721 ggttgacgtg gtaggcgagc tgctcggggc tgctcgtcgt gtagacgccg accgtagcgc
      781 cgccagccgt ctggatcgcg atgtcggcga tgagccattc cggccggttc tcgcagagga
      841 tggccacgcg ctcgccctgc tcgagcccga ggtggatcat ctcgcgcgcg atggcgttga
      901 cgcgcccggc gtactcggtc cagctgatcc tctcccagag cccgaggcgc ttgacgcgca
      961 gagcggtccc ggggtcggcc gcgcgcaggc gcaggagcgc cgggacgctg cgcgcgtcct
     1021 gggcgccccc tgcgccgcgc ggcgcggcag tagcgtgcgc gctcacgtgg gtgtcaggca
     1081 ttccgacgcg actatacccg tcgtcgtcgc ccgtcgctca cgggagaacc cgtcgtcagg
     1141 agaccgcctc cccgccggcc cgacaagggc gcaaggcgct gccggcatgt tcggcgctaa
     1201 cgagcggggc ccttcggcta accgggcgcc agcgccggct ccggccgccc gagcgcccga
     1261 gccgcctccg gccacccgag cgcgcgcgcc gcctcgatgg cgagctgcgc gcaccagacc
     1321 ccgatgcgcg cttccgtgag gtcgtggtcg ttgtcctggt cgagcgcgag accgacgaag
     1381 ctgtcgccgc gcagcgcgag cgagcctgtg aagcggtagc cggccgtcga ccactcgcct
     1441 agcagagtgg cccccgccgc cgtcgcggcc ttggcgatgg cgtccagcgc gtcgaggtac
     1501 gtgtccggat agccgcgcgc gtcgcccgta ccgaagaggg cgacgagctt gccggtcagg
     1561 tcgagg
//
LOCUS       DAOQYE010000587         1566 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_98728_length_1566_cov_2.706155, whole genome shotgun sequence.
ACCESSION   DAOQYE010000587 DAOQYE010000000
VERSION     DAOQYE010000587.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98728_length_1566_cov_2.706155"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="PKN52_09210"
     CDS             <1..282
                     /locus_tag="PKN52_09210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00169.1"
                     /translation="ALMSSSMRTVIYGLVAFVGLGLFFELFIFESRGAFMRWVLPIIL
                     IAAGAVVLYLNNRNRPQAGVPVSAAPASPSSGAAAKPPAQQITDEGTVD"
     gene            340..>1566
                     /locus_tag="PKN52_09215"
     CDS             340..>1566
                     /locus_tag="PKN52_09215"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNR00170.1"
                     /translation="MRLIVTHEQPDFDALASLALARLLYPGSVATIHGALSRQLSAFL
                     RLYKDELDLTPSDQVDVGAVTELVVLDTNDADRIKPFDALVGRVPVTLYDHHPRTAAS
                     IAAARGISERLGSTAALLTRELMATAITIPPQVATLALLGIHEDTGNLTYDQTTPDDY
                     RAAEHLLTCGANLQLVRRFAHGALDADQTAFREALLANTELVHVAQRPVAAAAFEYPK
                     YVAGVSGTVNDLLDLFAADAAVAAVGMEGRTLVFARSGGRFDCAAALAGTVSGGGHPG
                     AAFGRSDAAPAEALEAVLSALAANATPTILAADLMSSPVRSVTPDAPVAEAADQLLLF
                     GHNGMPVVDGSGRVVGIVSRRDLDRATRHGLSDSRVSGFMSRDVISAPPTASLEELEA
                     LVLEHNIGRIPIVEGDR"
BASE COUNT          230 a          599 c          510 g          227 t
ORIGIN      
        1 gccctgatga gttcgagcat gcgcacggtg atatacggtc tcgtcgcctt cgtgggcctc
       61 gggctcttct tcgagctgtt catcttcgag tcgcgcggcg cgttcatgcg ctgggtgttg
      121 cccataatcc tcatcgccgc cggcgccgtc gtcctctacc tgaacaaccg caaccgtccg
      181 caggccggag tgcctgtcag cgcggcaccc gcgagcccaa gcagtggagc agccgccaaa
      241 ccgccagcgc agcagatcac ggacgagggc acggttgact agtgggctag gcctgcgccg
      301 aggcgccgcg ccacgcaagg ccctcccgcg cggtgccccg tgcgcctgat cgtcacgcac
      361 gagcagcccg acttcgacgc gctcgccagc ctggcgctgg cccggctcct ctacccgggc
      421 tcggtcgcca ccatccatgg tgcgctcagt cgtcagttga gcgcgttcct acgcctctac
      481 aaggacgagc tcgacctcac gccctccgac caggtcgacg tcggcgcggt cacagagctc
      541 gtcgtcctag acacgaacga cgcggaccgc atcaagccct tcgacgccct cgtcgggcgc
      601 gtgcccgtca cgctctacga tcatcacccg cgaacggcgg ccagcatcgc ggcggcgcgt
      661 gggatcagcg agcggctcgg ctccacggcc gccctcctca ctcgcgagct gatggcgacg
      721 gccatcacca tcccgccgca ggtcgccacc ctggcgctcc tcggcatcca cgaggacacg
      781 ggcaacctca cctacgacca gacgacgccg gacgactacc gggccgctga gcacctgttg
      841 acctgcgggg ccaacctgca actcgtcagg cgcttcgccc atggcgcgct cgacgccgac
      901 cagacggcgt tccgcgaggc gctgctggcc aacaccgaac tcgttcacgt cgcgcagcgc
      961 cccgtcgcgg cggcggcctt cgagtacccg aagtacgtcg ccggggtcag cggcacggtc
     1021 aacgacctgc tcgacctgtt cgccgccgac gccgccgtgg cggccgtcgg gatggaaggc
     1081 cgcacgctcg tgttcgctcg cagcggcggc cggttcgact gtgcggccgc gctggccggg
     1141 accgtgagcg gcggcgggca tcctggcgcg gccttcggcc ggtcggacgc cgcgccggcc
     1201 gaggcgctcg aagcggtgct gagcgcgctg gcagccaacg ccaccccaac catcctggcg
     1261 gctgacctca tgagcagccc agtgcgaagc gttacgccag acgctccggt ggcggaagcc
     1321 gccgaccagc tcctgctctt cggccataac ggcatgccgg tcgtcgacgg ttccgggcgg
     1381 gtcgtcggga tcgtctcgcg ccgcgacctc gaccgggcca cgcgtcacgg cctgagcgac
     1441 tcgcgcgtca gcggcttcat gagccgcgac gtcatcagcg cgccgcccac ggcatcgctc
     1501 gaggagctcg aagcgctcgt gctcgagcac aacatcggcc gcatcccgat cgtcgagggc
     1561 gaccgg
//
LOCUS       DAOQYE010000588         1566 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_98734_length_1566_cov_2.603574, whole genome shotgun sequence.
ACCESSION   DAOQYE010000588 DAOQYE010000000
VERSION     DAOQYE010000588.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98734_length_1566_cov_2.603574"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            115..1203
                     /gene="mnmH"
                     /locus_tag="PKN52_09220"
     CDS             115..1203
                     /gene="mnmH"
                     /locus_tag="PKN52_09220"
                     /inference="COORDINATES: protein motif:HMM:TIGR03167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016785 - selenotransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HNR00171.1"
                     /translation="MEIAPQDVYPLGGRVSPYAVVDVRSPIEVGRGALPGAVSLPLMT
                     DTERHLVGIRYKEAGQRAAIELGYELVGDSLAERTSAWREAIDGSQRPSAVMCWRGGL
                     RSKLVVEFADRERAERVAGGYKALRGHLMATLPTALANKRLLVLSGMTGAGKTRLLRR
                     LQAAARVDAAALQVLDLEGLARHRGSAFGHVESPQPGQQTFENALAVELVLDPAQRIV
                     VEDESRYVGVRTVPDPLFAAMCSAHVVMLETSLAERGRAIYDEYVAAPTADRGREAVA
                     ADLAGSSLKLKRRLGAAGAQEVAARIAALAESEAAWADPEAHAGWIGALLEGHYDPLY
                     RRSLVKLARPVAFAGNEEEVSGWLRTEW"
     gene            1182..>1566
                     /locus_tag="PKN52_09225"
     CDS             1182..>1566
                     /locus_tag="PKN52_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HNR00172.1"
                     /translation="MAEDRVVSGHATAIKVSVQGVDEAASERAEQQAPWTPVRLKSTV
                     KKGGCAAKIAAGTLSQLVRSLPVGTHPDLIVGTEHLDDAAVWRMTPDLALIQTLDFFT
                     PILDDPRDFGAVAAANAISDVFAMGG"
BASE COUNT          220 a          546 c          572 g          228 t
ORIGIN      
        1 accgtgctgg tcaccacttc cggcatcgag ctgctcacgg actacccacg cgacctggcg
       61 agcctcacgc tcgacgcctg agcttcccgc cgaccgcagg gctatcctga cggggtggag
      121 atagctcccc aggacgtcta cccgctcggt ggccgcgtct cgccttacgc cgtcgtagat
      181 gtgcgctcgc ccatcgaggt ggggcgcggg gcgttgcctg gtgctgtgtc gctgccgctc
      241 atgacggaca cggagcggca cctggtcggc atccgttaca aggaggccgg ccagcgggcg
      301 gccatcgagc tcggttacga gctggtcggc gactcgctcg ccgagcggac ctccgcttgg
      361 cgcgaggcga tcgacggcag ccagcggccc tcggccgtga tgtgctggcg gggtggactg
      421 cgcagcaagc tcgtagtcga gttcgcggac cgcgagcgcg ccgagcgggt cgcggggggc
      481 tacaaggcgc tccgcggcca cctgatggcc acgttgccta ctgctctggc caacaagcgg
      541 ctgctcgtgc tcagcggcat gacgggggcg gggaagacgc ggctcctcag gcgcctgcaa
      601 gccgccgccc gtgtcgatgc cgctgccttg caggtcctcg atctggaggg cctggcgcgc
      661 caccggggca gcgctttcgg gcacgtggag agcccccaac ccggccagca gacgttcgag
      721 aacgcgctcg ccgtcgaact cgtgctcgac ccggcgcagc gcatcgtcgt cgaggacgag
      781 tcgcgctacg tcggcgtgcg caccgtgccg gacccactgt tcgcggccat gtgctccgcg
      841 cacgtcgtga tgctggagac gagcctggcc gagcgcggta gggcgatcta cgacgagtac
      901 gtcgccgcgc ctaccgctga ccgtgggcgc gaggccgtgg ccgctgacct cgcgggctcg
      961 agcctgaagc tcaagcggcg cctgggcgcg gccggcgcgc aggaggtcgc ggcccggata
     1021 gcggcgttgg ccgagagcga ggccgcctgg gcggaccccg aggctcatgc cggctggatc
     1081 ggcgcgctgt tggagggcca ttacgacccc ctctaccgcc gctcgctcgt taagctggcc
     1141 aggcccgtgg cgttcgcggg caatgaggag gaagtgagcg gatggctgag gaccgagtgg
     1201 tgagcgggca tgcgaccgcg atcaaggtga gcgttcaggg tgtggacgag gcggctagcg
     1261 agcgggcgga gcagcaggcc ccctggacgc cggtgcgcct caagagcacg gtcaagaagg
     1321 gcggctgcgc cgccaagatc gcggcgggca cgctctcgca gctcgtgcgc tccctgccgg
     1381 tcggaacgca ccccgacctc atcgtcggca ccgagcacct ggacgacgcc gcggtctggc
     1441 gcatgacccc cgacctggcc ctgatccaga cgctcgactt cttcacgccc atcctggacg
     1501 acccgcgcga cttcggtgcc gtggcggccg ccaacgccat ctccgacgtc ttcgccatgg
     1561 gcggcc
//
LOCUS       DAOQYE010000589         1559 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_99455_length_1559_cov_3.123005, whole genome shotgun sequence.
ACCESSION   DAOQYE010000589 DAOQYE010000000
VERSION     DAOQYE010000589.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99455_length_1559_cov_3.123005"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="PKN52_09230"
     CDS             complement(<1..388)
                     /locus_tag="PKN52_09230"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00173.1"
                     /translation="MDLSLLPQAVVSGVLASGLYALVSVGLALAIGVIGIVNFAHGEF
                     FMVGAFLAYQLFVSFGFDPLLSLLFVAPALAVIGALIYLSSIRFVLKAPELNQMLLTF
                     GIGIILQNLALLIWGGRNGNGPRSGRA"
     gene            complement(435..>1559)
                     /locus_tag="PKN52_09235"
     CDS             complement(435..>1559)
                     /locus_tag="PKN52_09235"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNR00174.1"
                     /translation="IGVLSPLTGGAAGTGQAQKAGFELALAEINAAGGVLGQPLKIVI
                     EDTKADAATGVAAFEKLMTEDNVEFIAGGYSSGVTLALVESFRTFQPVVSWIGGAVSG
                     VGIDGFDGIEELLGAEPWFFHVHPWDYQNTEASTSFMASTGVKSVALLHEDSAFGGPG
                     AIAAAGLLQNAGMSVPLVEAFKSTLTGGTGDFRATIAKAAASGADALFWIGYDSDVVP
                     LTSQIRELKYDPQYIFGAPPGWPSEFDAAPEAQCVAGLIGYLPNLPNPEAKTFLDSYV
                     AMHGKAPDNYMAALAYAQLWSYADAINAAGSTDQDAVIAALESGTFKSPMGEWSFKPS
                     VIALHQGFGSEMWNVFQFQNGVREIVWPLARATAPLQSCR"
BASE COUNT          271 a          477 c          575 g          236 t
ORIGIN      
        1 aagcgcggcc ggatcggggt ccgtttccat tcctgccgcc ccagatcagg agggccaggt
       61 tctgcaggat gatgccgatg ccgaacgtca ggagcatctg gttgagttcc ggcgccttca
      121 gcacgaagcg gatggaggat agatagatga gcgcgccgat cacggcgagg gccggcgcca
      181 cgaagagaag cgacagcagc gggtcgaacc cgaagctgac gaagagctga taggcgagga
      241 acgcgcctac catgaagaac tcgccgtgcg cgaagttgac tatcccgatg acgccgatgg
      301 ctagcgccag cccgacggac acgagcgcgt agagcccgga ggcgagcacg ccagacacga
      361 cagcttgggg taggagagat aggtccatga gggagagggt gggggcggcc ttgtgccgcc
      421 cccggatgat cagctcagcg gcagctctgc agtggcgccg tggcgcgcgc gagcggccag
      481 acgatctcgc ggacgccgtt ctggaactgg aagacgttcc acatctccga gccgaagccc
      541 tggtgcaggg cgatgacgga cggcttgaag ctccactcgc ccatgggcga cttgaaggtg
      601 ccgctctcga gcgccgcgat cacggcgtcc tggtcggtcg agccggcggc gttgatcgcg
      661 tcggcgtagc tccagagctg ggcgtacgcg agcgcggcca tgtagttgtc tggcgccttg
      721 ccgtgcatgg ctacgtagga gtcgaggaag gtcttggcct cggggttggg gaggttgggc
      781 aggtagccga tcaggccggc gacgcactgc gcttctggcg ccgcgtcgaa ctcgctcggc
      841 catcccggag gggcgccgaa gatgtactgc gggtcgtact tgagttcgcg gatctggctc
      901 gtgaggggca cgacatcgga gtcgtagccg atccagaaga gcgcgtcggc gcccgaggcg
      961 gccgccttgg cgatggtcgc gcggaagtcg ccggtgccgc ccgtgagggt cgacttgaag
     1021 gcctcgacga gcgggacgct catcccggcg ttctgaagca ggccggcggc agcgatggcg
     1081 ccgggaccgc cgaacgcgga gtcttcgtgc agcagggcca cgctcttcac gccggtgctg
     1141 gccatgaagg acgtgctcgc ctcggtgttc tggtagtccc acggatggac gtggaagaac
     1201 caaggctccg cgcccagaag ctcctcgatg ccgtcgaagc cgtcgatgcc gacgccggag
     1261 acggcgcccc cgatccacga cacgacgggc tggaaggtgc ggaaggactc gacgagcgct
     1321 agggtcacgc cggacgagta gccgcccgcg atgaactcca cgttgtcttc cgtcatgagc
     1381 ttctcgaagg ccgcgacacc ggtggcggcg tcggccttcg tgtcctcgat gacgatcttg
     1441 agaggctggc caagcacgcc gccggcagca ttgatctcgg cgagagcgag ctcgaacccg
     1501 gccttctgcg cttggccggt gccggccgcg ccgcccgtga ggggcgagag cacgccgat
//
LOCUS       DAOQYE010000590         1558 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_99587_length_1558_cov_2.681304, whole genome shotgun sequence.
ACCESSION   DAOQYE010000590 DAOQYE010000000
VERSION     DAOQYE010000590.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99587_length_1558_cov_2.681304"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="PKN52_09240"
     CDS             <1..592
                     /locus_tag="PKN52_09240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00175.1"
                     /translation="LDMTDCRYEQYDERTTRVTGPRFVKADKVRVKLEGSGKVGERYM
                     GFAGVRDPYTIARIDEVIDWARQQVRERFGEPETGGYELHYQVYGRDGVMGEMEPLRD
                     SPGHELGIMVFGVAPTAEMAEEVALTGTRQMFYARLPDVKGTAGGVAFPLDEVTRVSP
                     AYRWTLNHTVAVDDPLELFELHLAEAGVAAVGSGGR"
     gene            592..915
                     /locus_tag="PKN52_09245"
     CDS             592..915
                     /locus_tag="PKN52_09245"
                     /inference="COORDINATES: protein motif:HMM:NF025688.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4387 domain-containing protein"
                     /protein_id="HNR00176.1"
                     /translation="MASKTLSELAKTIRSKNAGVDRITFDIIFRDRENYELVKESGVL
                     GRDSVARLYRIDPARITDFVNYDPAFAIKFTILRNRPSGSAGDSDIFGAQQYAPLLGV
                     EVPID"
     gene            984..>1558
                     /locus_tag="PKN52_09250"
     CDS             984..>1558
                     /locus_tag="PKN52_09250"
                     /inference="COORDINATES: protein motif:HMM:NF013577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HNR00177.1"
                     /translation="MSSEQHADGLRGRLDEAASRLTPQERRLAAHLVEQQERWGYLSS
                     TELAKDLGVHRSTIVRFAQGLGFKGFPELQEQARAAYLNTVSLQRELVLSDVGSQESD
                     TVQAVYRREMQNLQQSYQHLDLAALEATAGRLAGARRVLLFGRRFSHPIALHLGLVLR
                     TMREGVEVAPPVSGTAVDLLFDLGPEDFVLVV"
BASE COUNT          262 a          476 c          561 g          259 t
ORIGIN      
        1 gctcgatatg acggactgcc gctacgagca gtacgacgag cgcaccacgc gcgtgaccgg
       61 accgcggttc gtgaaggccg acaaggtgcg ggtcaaactc gaaggctccg gcaaggtggg
      121 cgagcgctac atgggcttcg ccggcgtgcg ggacccgtat accatcgcgc ggatagacga
      181 ggtcatcgac tgggcgaggc agcaggtcag ggagcggttc ggtgagccgg agaccggggg
      241 ctacgagctg cactaccagg tctacggcag ggacggcgtc atgggcgaga tggaaccctt
      301 gcgggacagc ccggggcacg agttggggat catggtgttc ggcgtggcgc cgacggcgga
      361 gatggctgag gaggtcgccc tcaccggcac gcgccagatg ttctatgcgc ggctgccgga
      421 cgtgaagggc acggcgggcg gggtcgcctt cccgcttgac gaggtgacgc gcgtgagccc
      481 cgcgtaccgg tggacgctga accacaccgt ggcggtcgac gatccgctcg agttgttcga
      541 gttgcatttg gccgaggccg gcgtcgcggc ggtagggtca ggcggtcgtt gatggcaagc
      601 aagacgctct cggaactagc gaagacaatc cgtagcaaga acgccggtgt cgaccggata
      661 acgttcgaca tcatcttccg cgaccgcgag aactacgaac tcgtgaagga gagtggggtc
      721 ctgggtcgcg actcggtcgc gaggctctac cggatagacc cggcgcgtat aaccgacttc
      781 gtgaactacg acccggcctt cgccatcaag ttcaccatcc ttcgcaaccg cccgagcggc
      841 agcgccggcg actcggacat cttcggcgcc cagcagtacg cgcccctgtt gggcgtggaa
      901 gtgccgatcg attgagggcc atggccaggt tcttcaggta gcttgcgggc gtccacgggt
      961 cgtggccggg taggctgtgt cgcatgagtt ccgagcagca tgcggacggt ctgcgcggcc
     1021 ggctcgacga ggccgcctca cgcctgacgc cgcaggaacg ccgcctggcg gcccacctgg
     1081 tggagcagca ggaacgttgg ggatacctgt ccagcacgga actcgccaag gaccttggtg
     1141 tgcatcgctc caccatcgtg cggttcgcac aggggcttgg tttcaagggt tttcccgaac
     1201 tgcaggagca ggcgcgggcc gcctacctga acactgtttc gctgcagcgc gagctcgtcc
     1261 tgagcgacgt cggctctcaa gagagcgaca cggtgcaggc cgtctacagg cgcgagatgc
     1321 agaacctgca gcagagctac cagcaccttg acctggccgc gctcgaggcc accgccggtc
     1381 gtttggcagg ggcccgcagg gtgttgctgt tcgggcggcg cttctcccat ccgatcgctt
     1441 tgcaccttgg gctcgtgctg cgcaccatgc gagagggcgt cgaggtggcc ccgccggtca
     1501 gcggcacggc ggtcgacctc ctgttcgacc tgggtccgga ggacttcgtg ctggtggt
//
LOCUS       DAOQYE010000591         1555 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_99887_length_1555_cov_2.592000, whole genome shotgun sequence.
ACCESSION   DAOQYE010000591 DAOQYE010000000
VERSION     DAOQYE010000591.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99887_length_1555_cov_2.592000"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="PKN52_09255"
     CDS             <1..294
                     /locus_tag="PKN52_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00178.1"
                     /translation="AQLFKKVANGVVLVLTEDGIGSGSLITPEGDIITNWHVVGDYKV
                     VGVVFRRRRTRPARRQDDRAGIAPGRLSPLSREPSTEATPPLAWVTVCRFRRA"
     gene            296..>1555
                     /locus_tag="PKN52_09260"
     CDS             296..>1555
                     /locus_tag="PKN52_09260"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HNR00179.1"
                     /translation="MTWLLKLDKTSRLIFLSIVVGFVGAGGAHLFLWMVNVASDLLIV
                     GIAGMPALEPEQAVALGTSPVQPVLNWLIPVSTALGGLLVGLLIVALAPEAEGHGTDA
                     AIRSFHRLGGYVRTRIPIVKTVASAITIGSGGSAGREGPTAQIASGFGAITATWLRLP
                     DDERRTLMLAGMAAGLSAIFRSPLGTAIFAVEVLYSSLAFESAALVFTFIGAATAYAV
                     SGTFTGFAPLFELSAGITFTGVRELGWYALLGVAAGLLAVLVPLVYYAVRDWFSALKL
                     PTFLKPALGGLAMGLIGMVFPQLLGSGYGWMQLALDGHLGLWLMLVLALGKLVTMALT
                     IGSGGSGGTFAPSLYVGVMLGGFMAAALGAVTPSAPPPQAFAVVGMAAVFAGAARVPL
                     ATLIMVAEMTGGYQLILPTMLAVALSFM"
BASE COUNT          193 a          569 c          526 g          267 t
ORIGIN      
        1 gcgcagctct tcaagaaggt cgccaacggc gtcgtcctgg tgctgaccga ggacggcatc
       61 ggctccggct cgctgatcac gccggagggc gacatcatca ccaactggca tgtggtgggc
      121 gactacaagg tcgtcggcgt cgtcttccgc cgcaggcgca cacgtccagc ccggcggcag
      181 gacgaccggg cgggcatcgc gccgggccgg ctctcgcccc tgagccggga gccgagcacc
      241 gaggccacgc cgccgctagc gtgggttact gtatgccggt tccgaagggc ttaacgtgac
      301 ctggctcctc aaactcgaca agacctccag gctgatcttc ctcagcatcg tagtcggctt
      361 cgtcggcgcg ggcggcgcgc atctgttcct ctggatggtc aacgtcgcca gcgacctgct
      421 catcgtcggc atcgcgggca tgcccgccct cgagccggaa caggcggtag cgctcggcac
      481 atccccggtt cagcccgtgc tcaactggtt gatcccggtg tcgacagcac tcggcggcct
      541 gctcgtcggg ctgctgatcg tcgcgctcgc gccggaggcg gaggggcacg gcactgacgc
      601 cgccatccgc tcgttccacc gcctcggcgg ctacgtgagg acgcgcatcc ccatcgtcaa
      661 gaccgtagcg agcgccatca cgatcggctc cggcggctcg gcgggccgcg aggggccgac
      721 ggcgcagatc gcgtccggct tcggcgccat caccgccacc tggctgaggc tccccgacga
      781 cgagcggcgc accctcatgc tcgcaggcat ggcggcgggc ctgtcggcca tcttccgcag
      841 cccgctcggc acggcgatct tcgccgtcga ggtgctgtac agcagcctcg ccttcgagag
      901 cgccgcgctc gtgttcacgt tcatcggcgc ggccacggcc tacgcggtca gcggcacctt
      961 cacgggcttc gctcccttgt tcgagctgag cgccggcatc acgttcaccg gcgtgcgcga
     1021 actcggctgg tacgcccttc tcggcgtggc ggccgggctg ctggccgtgc tcgtgccgct
     1081 cgtctactac gccgtgcgcg actggttctc cgcgctgaag ctgcccacct tcctgaagcc
     1141 ggcgctgggc gggctcgcca tgggactgat cggcatggtg ttcccgcagc tcctcggcag
     1201 cggctacggc tggatgcagc tcgcgctcga cggtcacctg gggctatggc tgatgctcgt
     1261 cctggcgctc ggcaagctcg tcaccatggc cctgaccatc ggttccggcg gctcgggcgg
     1321 caccttcgcc ccgagcctgt acgtgggcgt catgctcggc ggcttcatgg ccgccgccct
     1381 tggcgccgtc acgccctccg cgccgccacc gcaggcgttc gcggtggtcg gcatggcggc
     1441 cgtgttcgcc ggggccgcgc gggtcccgct cgccacgctg atcatggtgg cagagatgac
     1501 gggcggttac cagctcatcc tgcccaccat gctggccgtg gcgctgagct tcatg
//
LOCUS       DAOQYE010000592         1555 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_99935_length_1555_cov_1.546000, whole genome shotgun sequence.
ACCESSION   DAOQYE010000592 DAOQYE010000000
VERSION     DAOQYE010000592.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99935_length_1555_cov_1.546000"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(260..1129)
                     /locus_tag="PKN52_09265"
     CDS             complement(260..1129)
                     /locus_tag="PKN52_09265"
                     /inference="COORDINATES: protein motif:HMM:NF024313.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNR00180.1"
                     /translation="MSEAKTSRRRLTGLRMLLRSRTATAGAVILVCLAVLAALAPQLS
                     PHDPARQDLRARLAPPAWVAKGTNAHVLGTDQLGRDILSRVIYGTRISLLVGVSVVLL
                     AGTLGTLLGLLAGFLGGKVDTVLMRIVDVFLAFPFLLLAIVFMAMLGASLGNIILVLA
                     ITGWVEYARVVRAQVLSVRELEYVTAARALGARNLMIMLKHVLPNVFASVIVIASLQL
                     GTVIISEASLTFLGLGIPPSIPTWGSMLASGREYFVLAWWLPTFPGLAIVLTVLAVNF
                     LGDWLRDVFDPTL"
     gene            complement(1122..>1555)
                     /locus_tag="PKN52_09270"
     CDS             complement(1122..>1555)
                     /locus_tag="PKN52_09270"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNR00181.1"
                     /translation="PSGFGTWKHLVMPAVALSAGLAAMVARLTRSSLLEVMGHDFVRT
                     AKAKGLTESRVLYRHVMRNALIPIATVVGLQAGHLLGGAIITETIFAWPGVGRLLTQS
                     IYNYDYPVVQTSVFVISVTFVFLNLAIDLLYALIDPRIRYE"
BASE COUNT          288 a          453 c          582 g          232 t
ORIGIN      
        1 gtacacgtat tgcggctggc tcaccatcag catcgggccg ggctgggcgc cgtgaagcgt
       61 gtggatggcc aggctgaggg tctcaccgga ggcgcgggtg gtgacgggcg cccagcggtg
      121 ggagcggccg ggtttctggc tcgtaggggt tgcgagtgcc atgcgtactc ctatgactgg
      181 acggcggtgg aagtctcgtg tgagagccgg ccgccagcgt gtggtggtct tgttcgaggg
      241 accgtggtcg agcgttccgc tagagcgtag ggtcgaacac gtcgcgcagc caatctccga
      301 ggaagttgac ggccagcacc gtgaggacga tggcgaggcc ggggaaggtg gggagccacc
      361 aggccaggac gaagtactcc cgacccgacg cgagcatcga gccccacgtg gggatagagg
      421 gcgggatgcc cagtccaagg aacgtgagcg aggcctcgga gatgatgacc gtgcccagtt
      481 gcaggctggc gatgacgatg acggaggcga acacgttggg cagcacgtgc ttgagcatga
      541 tcatcaggtt gcgggcaccg agcgccctgg cggcggtgac gtactcgagc tcgcgcacgg
      601 acaggacctg cgctctgacg acccgggcgt actccaccca cccggtgatg gcgaggacca
      661 ggatgatgtt gcctaggctg gcaccgagca tggccatgaa cacgatcgcc aacaggagga
      721 acgggaacgc gaggaacacg tcgacgatgc gcatcaacac ggtgtcgacc ttgccgccca
      781 ggaagccggc gagcagaccg aggagcgtgc cgagcgtgcc ggcaagcagg acgacggaca
      841 cgcccaccag tagggagatg cgcgtcccgt agatgacgcg ggagaggatg tcccggccca
      901 actgatcggt gccgaggacg tgggcgttgg tgcccttggc gacccacgcc ggcggcgcga
      961 gcctagccct caagtcctgt ctggccggat cgtgaggcga cagctgtggg gccaacgccg
     1021 ccaggacggc caggcatacg aggatcacgg cccctgccgt cgcggtgcgg cttcgcagca
     1081 gcatgcggag gccggtcaga cgacgtctgc tggtcttggc ttcactcata gcggatgcgc
     1141 gggtcgatga gcgcatagag gagatcgatc gccaggttca ggaagacgaa ggtgaccgag
     1201 atgacgaaga cgctcgtctg cacgaccggg tagtcgtagt tgtatatgga ctgcgtgagc
     1261 agcctgccta cgcccggcca cgcgaagatg gtttcggtga tgatcgctcc cccgagcagg
     1321 tggcccgcct gaaggccgac gacagtggcg atggggatga gggcgttgcg catgacgtgc
     1381 cggtagagga cccgactctc cgtgagaccc ttcgcttttg ccgttcgcac gaagtcgtgg
     1441 cccatgacct cgagcaggct cgagcgggtg agcctggcga ccatcgccgc caggccggcg
     1501 ctcagggcga cggcgggcat cacaaggtgc ttccacgtcc cgaagccgga cgggg
//
LOCUS       DAOQYE010000593         1550 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_100397_length_1550_cov_3.024749, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000593 DAOQYE010000000
VERSION     DAOQYE010000593.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100397_length_1550_cov_3.024749"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1222)
                     /locus_tag="PKN52_09275"
     CDS             complement(<1..1222)
                     /locus_tag="PKN52_09275"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNR00182.1"
                     /translation="MKLRLQLTIVFGAFIAAILSAVAVSVYVLTERSLSVGITDRAER
                     ALGELTSGTSTIDQGLQKLPSDTYYQILIIGLAGRVPESITSLRSGVTYKSNTSLIGT
                     LSDAAAQTLLATGEVREVVNSKGERIQVLARLAAITFPAQDSTVQAAFLVGIRASLIA
                     RTLDQLARDLIATVLIAFLVFALGVWLLSERVLGPLKRVTAAASKVSGSELGQRVPVP
                     TNRDEIRELGVTINHMLDRLQESFETQRRFTADASHELRTPVTAIGGHVNYLLRRTGP
                     TPDQVESLEVIRRESERMAKLVNDLLELARADAGFTVHLEPMNLVEVVEAVKQEVAPV
                     AGGSKIHVRSSTPLAEVMGDFTRLKQVVLNLVQNALNAGAKNVTVSLRPERSQVHLEV
                     LDDGAGIPAEALPHL"
     gene            complement(1249..>1550)
                     /locus_tag="PKN52_09280"
     CDS             complement(1249..>1550)
                     /locus_tag="PKN52_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HNR00183.1"
                     /translation="TGEVRVVDLVMNLNGREVFRDGRRIELSAKEFELLELFARNPGK
                     VFSRFEIEEKVWPEYTGGSNVVDVYVGYLRRKLEQEGERRLVHTVRGVGYVLREE"
BASE COUNT          239 a          502 c          546 g          263 t
ORIGIN      
        1 agaggtgcgg caacgcctcg gccgggatgc cggcgccgtc gtcgaggacc tcgaggtgca
       61 cctgggagcg ctcggggcgc aggctcaccg tcacgttctt ggcgccggcg ttgagcgcgt
      121 tctggacgag gttgagtacg acctgcttga ggcgggtgaa gtcgcccatc acctccgcga
      181 gcggggtgga gctgcgcacg tggatcttcg agccgcccgc gacgggtgcg acctcctgct
      241 tgacggcctc cacgacctcg acgaggttca tcggctcgag gtgcacggtg aacccggcgt
      301 cggcgcgggc gagttcgagc aggtcgttga ccagcttcgc catgcgctcg ctctcgcgcc
      361 tgatgacctc gagggactcg acctggtcgg gggtcggccc ggtgcgcctc aggaggtagt
      421 taacgtgacc gccgatggcc gtgacgggcg tgcgcagctc gtggctggcg tcggccgtga
      481 agcgccgctg cgtctcgaac gactcctgta gccggtcgag catgtggttg atggtcacgc
      541 ccagttcacg gatctcgtcg cggttggtgg ggaccggcac gcgttgcccc agctcggagc
      601 cggagacctt cgaggccgcc gcggtgacac gcttgagcgg ccccaggacg cgttccgaga
      661 ggagccacac gcccagagcg aacacgagga aggcgatcag aacggtggcg atgaggtcgc
      721 gcgcgagctg gtcgagggtc ctcgctatca acgacgccct gatgccgacg aggaaggcgg
      781 cctgcacggt gctgtcctgt gccgggaagg tgatggcagc gagcctcgcg agcacctgga
      841 tccgctcacc tttggagttg accacctccc tgacctcgcc ggtggcgagg agggtctggg
      901 cggccgcgtc ggagagcgtg ccgatgaggc tggtgttgga cttgtaggtg acgccgctcc
      961 gcaagctggt gatgctctcg gggacgcggc cggccagccc gatgatgagg atctggtagt
     1021 aggtgtcgct gggcagcttc tgcaggccct gatcgatggt cgaggtcccg ctcgtcagct
     1081 ccccgagcgc gcgctccgcc ctgtcggtga taccgacgct gagggagcgc tccgtgagca
     1141 cgtagaccga caccgccacc gcggacagga tggccgcgat gaaggcgccg aagactatcg
     1201 ttagttgcag ccgcagcttc acttgtcgtt acccgcccaa gagcgcgctc actcctcgcg
     1261 cagcacgtaa ccgacgccac gcaccgtgtg cacgaggcgc cgttcgcctt cctgctcgag
     1321 cttacgccgc aggtagccga cgtacacgtc gacgacattg ctaccgcccg tgtactccgg
     1381 ccagaccttc tcctctatct cgaagcggct gaagaccttg cctgggttcc tggcgaagag
     1441 ctcgaggagc tcgaactcct tggcggacag ctcgatacgg cgcccgtcgc ggaagacctc
     1501 gcggccgttc aggttcatca cgaggtcgac cacccggacc tcgccggtga
//
LOCUS       DAOQYE010000594         1549 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_100541_length_1549_cov_2.560910, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000594 DAOQYE010000000
VERSION     DAOQYE010000594.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100541_length_1549_cov_2.560910"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="PKN52_09285"
     CDS             complement(<1..512)
                     /locus_tag="PKN52_09285"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNR00184.1"
                     /translation="MPVATLGLHHVTGIATRPQANIEFWVRLLGQRLVKRTVNYDDPQ
                     TYHLYFGGPTGQPGTVATFFPWQGAGPAVRGAGESVAVAYRVPPGGLTAWRRRLDDHG
                     LAHATFERFGEEVVAFQDPDGVPLELTEAAGGGPRLVWPDAPLPDGLTLQGLHAVTVA
                     APRLDATASVL"
     gene            733..1146
                     /gene="rplS"
                     /locus_tag="PKN52_09290"
     CDS             733..1146
                     /gene="rplS"
                     /locus_tag="PKN52_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HNR00185.1"
                     /translation="MKYNKGAILRSIEQPYLRNDIPDFDSGDTVRVDYKVVEGNRTRI
                     QAFEGVVIARKNGKGARSTVTVRKVSFGEGVERVFPLHSPLIEKIEVVRRGRTRRAKL
                     YYLRELRGKASRLKTDVARQEQDRAAARAAKGDKQ"
BASE COUNT          270 a          526 c          525 g          228 t
ORIGIN      
        1 aggacgctcg ccgtcgcgtc gagccggggc gcggccacgg tgacggcgtg cagcccctgg
       61 agggtgaggc cgtccggtag aggcgcgtcc ggccacacca ggcgcggtcc gcccccagcg
      121 gcctcggtca gctccagcgg cacgccgtcc gggtcctgga aggccaccac ctcctcgccg
      181 aagcgctcga acgtcgcgtg agccaggccg tgatcgtcca ggcggcgccg ccatgccgtc
      241 aggccgccgg gcggcacgcg gtaggcgacg gctacgctct cgccggcccc ccgaacggcc
      301 gggccggcac cctgccaggg gaagaaggtc gcgacggtgc ccggctgacc cgtggggccg
      361 ccgaagtaga ggtgataggt ctgcgggtcg tcgtagttca cggtgcgctt caccagtcgt
      421 tggccgagca ggcgcaccca gaactcgatg ttggcttgag gcctcgtggc gatgccggtg
      481 acgtggtgga ggccaagcgt ggcgacaggc atgccctaga gcctagcagt aggccggtca
      541 cgcgcccttg ggctattgaa ccttggcgcg ccgttcggct aagatggcaa ggcttgacgc
      601 atggaggccg gctcgcgtcg cgagttggcg ccgacccctg gtctgccgcc ctccgcgacg
      661 cgtccgctgc tacgcgccta ggcgccgcgg cgagaagtcc gaccgcgtaa ggtcgacgac
      721 ctggagacga agatgaagta caacaaaggt gcaatcctgc gctctatcga gcagccctac
      781 ctgcgtaacg acatccccga tttcgattcg ggcgacacgg tccgcgtcga ctacaaggtc
      841 gtcgaaggca accgcacccg tatccaggcg ttcgagggcg tcgtcatcgc ccgcaagaac
      901 ggtaagggcg cccgcagcac cgtcacggtg cgcaaggtgt cgttcggtga gggcgtcgag
      961 cgcgtcttcc ccctccattc gcccctgatc gagaagatcg aggtagtgcg ccgtggccgc
     1021 acgcgccgcg ccaagctcta ctacctgcgc gagctgcgcg gcaaggcttc gcgcctcaag
     1081 acggacgtcg cccgccagga acaggaccgc gccgccgcgc gcgccgccaa gggcgacaag
     1141 cagtaagcgc cggccgcagc gcggccccga tgcaacgaac ggagcccctc gctcgatcga
     1201 gcgaggggct cttcttggtg ccgacgcggc gtcggtctag ggtctgttca ctcttgacgg
     1261 cacgactcac tcttgacggc gcggcgtcag aagttgggta cgactgagac acgatcaagg
     1321 acgaaccagc agcctcctcc cacacgatga tcgaactcga caagacactc cacgggcacg
     1381 cgctcctcga cgactcaaga accgtttgcg caccttgccg gggttctagc cgtgcaacgc
     1441 agcggatgca acacggcttc cttccaatac gctgccgcct gggtcggcgc ggcgagcatg
     1501 ggaggtctag tttgaaaaac gacatgatat gcaacgaggc agttgcgca
//
LOCUS       DAOQYE010000595         1548 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_100631_length_1548_cov_2.772271, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000595 DAOQYE010000000
VERSION     DAOQYE010000595.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100631_length_1548_cov_2.772271"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1206
                     /locus_tag="PKN52_09295"
     CDS             <1..1206
                     /locus_tag="PKN52_09295"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNR00186.1"
                     /translation="QAVLYQHVYGTPTHPQELNPQVPTSLARLILALMEKDPNKRPTS
                     GWAVADTLRAIRSAELQRAPQRAQAGPGRTGAYPTGPTNAASLRRLWTLALDEGPQFP
                     SGLSSSAGFLLVGQRSDSLAVVRPADGGLQATFALHDEVSQPPLVMGDTLYVVSREGA
                     IRAIAWPRGEVAWQRLDEEIVGAAPYGDELILALGDGVARWTPAGEEVWRVPLGAPPA
                     AAPTLHAGLTLVPTRDGWLHAVRTEAGASAFKIEVGAVTTPPSAAGGLLFIPVRQGEV
                     HAFDLAKREVVWSYDLEGEQWSTPVAWQQYVYVVSWGQRLHCLALRSGDDVWTRPLPA
                     PVTAPPVVAAGVLYVVTEGGDLIVLDARSGRVLLLDRVANAAIQAPPLPLGERLFVAA
                     LDGTLAAYG"
     gene            complement(1228..>1548)
                     /locus_tag="PKN52_09300"
     CDS             complement(1228..>1548)
                     /locus_tag="PKN52_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HNR00187.1"
                     /translation="SGDGPVNALDRGLLKALGAFYPALRGLTLRDYKVRVLSGPETGT
                     ASVVRVQVEMGDGTTTWSTVGASTNIIEASYEALLDAYEYKLVTDGVAPVLTDRDRGL
                     AASA"
BASE COUNT          192 a          585 c          555 g          216 t
ORIGIN      
        1 caggccgtcc tctaccagca cgtctacggc acgccgacgc acccgcagga actcaacccg
       61 caggtgccga cctcgctggc gcgcctgatc ctggcgttga tggagaagga ccccaacaag
      121 cgcccgacct ccggctgggc ggtggcggac acgctgcgcg ccatccgctc ggccgagctg
      181 cagcgggccc cgcaacgcgc ccaggcgggc ccaggccgca ccggcgccta ccctaccggt
      241 ccgacgaacg ccgcctctct ccggcggctc tggacgctgg cactcgacga ggggccgcag
      301 ttcccgtcgg gcctctcctc ctccgccggc ttcctcctcg tcgggcagcg ctccgactcc
      361 ctcgcggtcg tgcgcccggc ggacggcggc ctccaggcga cgttcgctct tcacgacgag
      421 gtgagtcagc cgccactcgt catgggcgac acgctctacg tggtgagccg cgagggggcc
      481 atccgggcca tcgcgtggcc ccgcggcgag gtggcgtggc agcgcctcga cgaggagatc
      541 gtcggagcgg ccccctacgg cgacgagctg atcctcgcgc tgggggacgg ggtggcgcgg
      601 tggacgccgg cgggcgaaga ggtctggcgc gttccgctcg gggccccgcc ggcggccgcg
      661 ccaacgttgc acgccggcct gacgctcgtg ccgacgcgcg acggctggct ccacgccgtc
      721 cgtaccgagg ccggggcgtc ggccttcaag atcgaggtcg gggcggtcac caccccgcct
      781 tccgccgccg gcggcctgct gttcatcccg gtcaggcaag gcgaggtgca cgctttcgac
      841 ctcgcgaagc gcgaggtcgt ctggagctac gacctggaag gcgagcagtg gagcacgccg
      901 gtggcttggc aacagtacgt ctacgtcgtc tcctggggcc agcggctgca ctgcctcgcc
      961 ctgcgcagcg gcgatgacgt gtggacgcgc cccctccccg cccccgtcac ggcgccgccg
     1021 gtggtcgccg ccggcgtcct gtacgtggtg acggaagggg gcgacctcat cgtgctcgac
     1081 gcccgcagcg gccgggtcct gctactggac cgggtcgcga acgcggccat ccaggcgccg
     1141 cccttgccgc tcggcgagcg gctcttcgtg gcggcgctcg acggcaccct cgcggcctac
     1201 ggctagcgct cgcggcgccg ccagcgccta ggcgctggcc gccagcccgc gatcgcggtc
     1261 cgtcaggacc ggcgctaccc cgtccgtgac gagcttgtac tcgtaggcgt caaggagggc
     1321 ctcgtaagag gcctcgatga tgttcgtgct cgccccgacc gtgctccagg tagtggtgcc
     1381 gtcgcccatc tcgacctgca cacgtaccac gctcgcggtg cccgtctcgg ggccggagag
     1441 cacgcgcacc ttgtagtcgc ggagcgtgag gccgcgcagc gccgggtaga aggccccgag
     1501 cgccttgagt agaccgcggt cgagggcgtt gacggggccg tcgcccga
//
LOCUS       DAOQYE010000596         1547 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_100713_length_1547_cov_3.541555, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000596 DAOQYE010000000
VERSION     DAOQYE010000596.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100713_length_1547_cov_3.541555"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(12..935)
                     /locus_tag="PKN52_09305"
     CDS             complement(12..935)
                     /locus_tag="PKN52_09305"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: protein motif:HMM:NF003915.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA];
                     GO_process: GO:0097173 - N-acetylmuramic acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HNR00188.1"
                     /translation="MSVDERDGKRASPIATEAAAADYSGLDAWQAGDILTAITASNRR
                     AIEAVERAIPDLERAALGIEERLRTGGRLIYVGAGTSGRLGLQDAAELAPTFGFDDAT
                     TLLAGGDAAQSKAKEGAEDDIAAAVQDVDAAGISARDAVVGIAASGATPFTVAAIERA
                     KELGAFTVGIANNRGTPLLAVPHVGVLLETGPEVLAGSTRLAAGTAQKAALNALSTTA
                     LVRLGGAYENLMVGMRPVNAKLRVRAVKIVAQGAGVSEAVAAAALEAAGGDIRCAIVS
                     AKSGASVEAARAALFAQAGNVRAALKVLSNE"
     gene            complement(932..>1547)
                     /locus_tag="PKN52_09310"
     CDS             complement(932..>1547)
                     /locus_tag="PKN52_09310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00189.1"
                     /translation="LLPGLPQEAPAAAWRLARRVGARFTTTEHDLLLDLRPTLPPAPL
                     PAGWRVRDDDPDGAIAFVARAFPGRWAHEVASYVAAGATILTLVDERAGEAASRGAGG
                     GPPATKGFCAVFTGEERLVGPQLYWRQALEGPGVRVGGMGPLGLDAAARGGGLGLAIV
                     GAGADWLKGRGLTDAVINWTTLTTFYGKLGARVWRTYQRAEGPL"
BASE COUNT          191 a          581 c          563 g          212 t
ORIGIN      
        1 atggtggacg gctactcgtt gctcaagacc ttgagcgcag cgcgcacgtt gccggcctga
       61 gcaaacagcg cggcccgcgc cgcctcgacg ctcgcccccg acttggcgct gacgatggcg
      121 cagcggatgt cgccgccggc ggcctccagc gcggcagccg ccaccgcctc actcaccccc
      181 gccccctggg ccacgatctt caccgcgcgc acgcgcaact tggcgttcac ggggcgcatg
      241 ccgaccatca ggttctcgta ggccccgcca aggcgcacga gcgccgtcgt cgagagggcg
      301 ttgagggcag ccttctgcgc ggtgccggcc gccaagcgcg tgctgccggc gaggacctcc
      361 ggaccggtct cgagcagtac gccgacgtgc ggcacggcca ggagcggcgt gccgcggttg
      421 ttggcgatgc cgacggtgaa cgcgccgagc tccttggccc gctctatggc cgcgaccgtg
      481 aagggcgtcg cgccgcttgc cgcgatgccg accacagcgt cgcgcgcgct gatcccggcg
      541 gcgtcgacgt cttgcacggc cgccgcgatg tcgtcctcgg ccccttcctt cgctttgctc
      601 tgcgcggcgt cgccgccggc cagcaacgta gtggcgtcgt cgaagccgaa cgtcggggcg
      661 agctcggcgg cgtcctggag ccctaagcgg cccgaggtgc cggcgccgac gtagatgagg
      721 cgtccgcctg tccgtaggcg ctcctcgatg ccgagggcgg cccgctccag gtcggggatg
      781 gcgcgctcga cggcctcgat ggcccgtcgg ttcgaggcgg ttatggccgt caggatgtcg
      841 cccgcctgcc aggcgtcgag gccggagtag tcggccgcgg ccgcttccgt cgcgataggg
      901 gacgcccgct ttccgtcgcg ctcgtccacg ctcacagcgg tccttccgcg cgctgatacg
      961 tgcgccagac gcgcgcgccg agcttgccgt agaaggtcgt cagggtcgtc caattgatga
     1021 cggcatcggt caagccccgc cccttgagcc agtcggcgcc ggccccgacg atggccaggc
     1081 cgagtccgcc gccgcgcgcg gcggcgtcca ggccgagcgg tcccatgccg cccacgcgca
     1141 cgccagggcc ttccagcgcc tgccgccagt agagctgggg gccgacgagc cgctcctcgc
     1201 cggtgaagac ggcgcagaac cccttggtgg ccggcggacc gccccccgct ccccggctcg
     1261 ccgcctcccc ggcgcgctca tcgacgagcg tgaggatggt ggcgccggcc gctacgtagg
     1321 acgccacctc gtgcgcccac ctgccgggga acgcgcgcgc gacgaaggcg atggccccgt
     1381 cgggatcgtc gtcgcgcacg cgccagccgg ccggtagtgg cgcgggcggg agcgtcggcc
     1441 tgaggtcaag gaggagatcg tgttcggtcg tcgtgaagcg cgcgccgact cgcctcgcca
     1501 agcgccaagc ggcggcggga gcttcctgcg gcaggcccgg caggagg
//
LOCUS       DAOQYE010000597         1543 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_101203_length_1543_cov_1.756720, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000597 DAOQYE010000000
VERSION     DAOQYE010000597.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101203_length_1543_cov_1.756720"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="PKN52_09315"
     CDS             <1..182
                     /locus_tag="PKN52_09315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00190.1"
                     /translation="KSGVLGDATIADAAQGERMLNEIARAIAAVLVAMAEEPAGTSKT
                     APTPAHAPAPGAGSR"
     gene            475..1275
                     /locus_tag="PKN52_09320"
     CDS             475..1275
                     /locus_tag="PKN52_09320"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNR00191.1"
                     /translation="MNQLEGRRILVTGAGSGIGKAISLKLAARGAEVWLADLNASAAR
                     VSVAEAVAAGGNAHGIEVDVTRAASLAAAAEEVFAAAGRLDVLVANAGVSTMNHFLDV
                     TEEEWDFNFSVNARGTFLTIQTFAKRMITQDLIAGSDVRGKIVATASMASRQAAPLLV
                     PYSASKFAVVGLAQAVAKELAEHKVTVNAVNPGFVKTSMQEREVVWEAKLRGISADEV
                     VAEYLRMTPLGRIQTPEDVAKVVAFLAGPDSDFMTGEVVEVNGGAWVS"
BASE COUNT          294 a          484 c          508 g          257 t
ORIGIN      
        1 gcaagagcgg cgttctcgga gacgccacga tcgcggacgc ggcgcaaggc gagcgcatgc
       61 tgaacgagat cgccagagcg atagccgcgg tactggtcgc gatggcggaa gagccggcgg
      121 gcacgtctaa gaccgcgccg acgccggcac acgcaccggc gcctggagcc gggtcccgat
      181 gacaacggac ctgcgcaggc gcgttgagcg gcgggcatgc gtaaaccttc atgatcgctc
      241 gccgattgag taccgcaggc ggtattcggg gccggttggt ggtgtcttga cgcctggcgt
      301 gggcctgagt aatcttgcac aaacgctcgt gaatgctctg gttcgttcat caggccgaag
      361 ggccgatgtc atgagggcga tggacctgca gccaagtcgc gcggagccgg tagagacttg
      421 gggcgttccg gaatctagga caccacgctc gtctcttcgc ttaggaaggc agacgtgaac
      481 cagctcgaag gcaggcggat cctcgttacc ggagcgggct ccggcatagg caaggccatc
      541 tcgctaaagc tagcagcgcg cggcgcggag gtctggcttg cggacctgaa cgccagcgcc
      601 gcacgcgtgt ccgtcgctga agctgtggcc gctggcggca acgctcatgg catcgaggtc
      661 gacgtcaccc gggccgccag tctggccgca gcggccgaag aggtcttcgc ggctgcaggc
      721 agactcgacg tcctcgttgc gaatgcgggc gtgtccacca tgaaccactt cctggacgtc
      781 acggaggagg agtgggactt caacttctct gtcaacgccc gcggcacctt cctgaccatc
      841 cagaccttcg ccaagcgcat gatcacgcag gacctcatcg ctggtagcga cgtccgcggt
      901 aagatcgtgg ccaccgcctc catggcgtct cggcaagccg ctcctctact cgtaccgtac
      961 tcagcgtcga agttcgccgt cgtcggcctg gcgcaggcgg tcgcgaagga gttggcggag
     1021 cacaaagtga ccgtcaacgc cgtcaacccc gggttcgtga agacatccat gcaggaaaga
     1081 gaggtcgtct gggaagcgaa gctcaggggc atcagcgccg acgaggtcgt cgccgaatac
     1141 cttcgcatga cgcccctcgg ccgcatccag acccccgaag acgttgccaa ggtcgtagcg
     1201 ttcctcgctg gaccggactc cgacttcatg accggtgagg tcgtcgaggt caacgggggt
     1261 gcgtgggtct cgtgaatcgc agcccttcaa gcgtcgagcc gacgcgccga gagcggaggt
     1321 gagtcagcct atcctgtcgt ccacgctgag tagagccgca ttcaagctag accttaggag
     1381 gatcacagca tgaatggaat aaggcgagcc gcaacttaca cggccctggc gctattcgcc
     1441 atggtcctga gcgtagcccc cgcgcagaag agccagtacg caggcacgaa cctcaacttc
     1501 atcgtcgtct ccgaccagtt ctcggctgcc ctcgaggcac tgg
//
LOCUS       DAOQYE010000598         1542 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_101271_length_1542_cov_2.640215, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000598 DAOQYE010000000
VERSION     DAOQYE010000598.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101271_length_1542_cov_2.640215"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            127..1437
                     /locus_tag="PKN52_09325"
     CDS             127..1437
                     /locus_tag="PKN52_09325"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNR00192.1"
                     /translation="MPAVGLLLWLLLLDPPSSLSLGFPVFETLLVLASLPLLGGTMMG
                     GASEGRVLVGLSFYLRALGAGSFAWLAGPTSEVGAQFLWLLSYTMLALGAQLELDDEH
                     TDYLPIAVAITSLQLVSAALLLVLFRLGMLEEPYALVIVSLLTYVQLAVVLLVLVTSR
                     INQVAAERELRAWARLLDPVMDAQPHGAALERVLDETVQLVPGAQGIELHDHGVAGAL
                     TGYPYPLVAGGAEIGRLYLTRRPHRSSVLDTCAPMLASRLLLLGDRDRWAAAALTDPL
                     TGLLNRRGLEARAGLLVGQAKADVRPISVAMLDLDHFKRVNDVYGHGVGDDVLKVLAG
                     VLRAQLRPSDQAVRWGGEEFVVVLPGADADQAVEVLKRVRHELGVALVKPVAWPLAVS
                     VGIAGSVGPPRSEEELGRLVEEADAALVAAKREGRNRIVLSGTL"
BASE COUNT          200 a          546 c          541 g          255 t
ORIGIN      
        1 aagaccagcg gcaagccgct ggtggtggcg atcggttccc cgatggggaa ctggctgcac
       61 ggcaactagg gcggatgggt ccgctaaaac acgcccgccc tagttgtctt cttttagctg
      121 gccgccgtgc cggccgtcgg cctcctcctc tggctcctcc tcctcgaccc gccatccagc
      181 ctctcgctag gcttccccgt gttcgagacc ctgctcgtcc tcgcgtcgtt gccgctgctc
      241 ggcgggacga tgatgggcgg agcctctgaa gggcgtgtgc tcgtcgggct gtccttctac
      301 ctgcgcgccc tcggcgccgg ttcgttcgcg tggcttgccg ggccgacgag cgaggtaggc
      361 gcgcagttcc tctggctcct ctcgtacacg atgctcgcgc tcggcgcgca gctcgagctc
      421 gacgacgagc atacggacta cctgccgatc gccgtagcca tcacgagcct ccagttggtc
      481 agcgccgccc tcctgctcgt cctgttcagg ctgggcatgc tggaggagcc gtacgcgctc
      541 gtgatcgtct cgctcctgac atacgtgcaa ctggccgtcg tactcctcgt cctggtgacg
      601 agccggatca accaggtggc tgccgagcgc gaactgaggg cctgggcgcg gctgctcgac
      661 cccgtcatgg acgcgcagcc gcatggcgcc gcgctcgagc gcgtcctcga tgagaccgtg
      721 cagctcgtgc ccggcgcgca aggcatcgaa ctgcacgacc acggcgtggc cggcgcgctc
      781 acgggctacc cctaccctct cgtggccgga ggggccgaga tcgggcgcct ctacctaacg
      841 cgcaggccgc accgctcctc cgttctcgac acgtgcgcgc ccatgctcgc atcgcgcctg
      901 ctcctcctcg gcgaccgcga caggtgggcc gcggcggcgc tgaccgaccc gctcacgggg
      961 ctgctcaacc ggcgcggcct cgaggcgcgc gccgggctgc tcgtcgggca ggcgaaggca
     1021 gacgtccggc ccatcagtgt ggccatgctc gacctcgacc acttcaagcg tgtcaacgac
     1081 gtgtacggcc acggggtggg cgacgacgtg ctcaaggtgc tggctggcgt cctgcgcgct
     1141 cagttgcgcc cgagcgacca ggccgtccgc tggggcggcg aggagttcgt ggtagtgctg
     1201 cccggcgcgg acgccgatca ggccgtcgag gtcctcaagc gggtgcggca cgagctcggc
     1261 gtcgcgctcg tcaagccggt cgcgtggccg ctggcggtgt cggtcgggat cgccgggagc
     1321 gtagggccgc cgcggtcgga ggaggagctc gggcgtctcg tggaggaggc cgacgccgcg
     1381 ctggtggcgg ccaagcgcga gggtcgcaac cgcatcgtgc tctccggcac cttatgatgc
     1441 cggcatgcgc gagctcctct acaacgccgc caagcacttc aagcgcggca aggcgttgag
     1501 cgagcgaggc gactcgcgcg gcgccatggt cgcgtacgag ga
//
LOCUS       DAOQYE010000599         1539 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_101584_length_1539_cov_2.731132, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000599 DAOQYE010000000
VERSION     DAOQYE010000599.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101584_length_1539_cov_2.731132"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="PKN52_09330"
     CDS             complement(<1..397)
                     /locus_tag="PKN52_09330"
                     /inference="COORDINATES: protein motif:HMM:NF012677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00193.1"
                     /translation="MSQPLDRVLAAVERQASYRAECVGLVPTENLLSPAARSVLASDL
                     GHRYLFQNPQWRYVGGKHLSEVETLAQGLARELFDARHANVRPLSGENCTNIVIHSVL
                     RRGGTFYHLAMEDGGHFAARSVAARLTDDL"
     gene            complement(808..>1539)
                     /locus_tag="PKN52_09335"
     CDS             complement(808..>1539)
                     /locus_tag="PKN52_09335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00194.1"
                     /translation="AVNLYDLQLTDGEITDLSPLATLDSLTWVDLNINDISDIEPLRG
                     RTNIRGLHVCCTEAKITDTSALATLTSMWTMNLGFHGLGDALIWPLLENYPDIHGLWL
                     PGNDLTEFDHLLDYPQLDALDIGWHTIPNLSFLDVMDDLVFLDLRGAVIEDMSALTAK
                     TALTTLTLSTTGISDIAFLEAFTELENLNLDGNNIADLAPLVANPGIGEGDVVDVRFN
                     AFDPEDPAIAEQIQALLDRGVDLRY"
BASE COUNT          264 a          453 c          544 g          278 t
ORIGIN      
        1 gcaggtcgtc ggtgaggcgc gccgccacgc tcctggcggc gaagtgcccg ccgtcctcca
       61 tggcgaggtg gtagaaggtg ccgccccgcc tgagcacgga gtggatcacg atgttggtgc
      121 agttctcgcc cgagagcggg cgcacgttcg catggcgggc gtcgaacagc tcgcgcgcca
      181 gcccctgagc cagcgtctcc acctccgaca ggtgcttgcc gcccacgtag cgccactggg
      241 ggttctggaa caggtagcgg tggccgaggt cggatgccaa cacgctgcgg gccgccgggc
      301 taagcaggtt ctcggttgga acgagcccga cgcactccgc gcggtacgag gcctggcgtt
      361 cgaccgcggc cagaacgcgg tcgagaggct gactcacggg ctgttgagcc gcgatcgccg
      421 cctccgttcg cgctcgggtt ctcgcgtccg cggtaacgga gtcgctcatc taagggtccg
      481 tcgggcgtcg aatcgtggct ggaacggggg tcgtggcatg gcgtctccaa gtgttcaggg
      541 attattgatt tcgaaacgaa agctaggagc cacaactcca gccttctgag gaacgataac
      601 ataagtgagc ggccagcata gcctaagaaa cgaatggccc cctctgggcc agttccgggc
      661 tcatggggcc gctcccgctc acgatcgcgc tgggagacgc aactcgctcg cggctgccga
      721 cgagcgcacg tcgtttggcg gggtgaacga gaccggggtg gagcttgatg gctccacccc
      781 agcgcatgcc gtcgttgaag aagcgggtca gtagcgcagg tcgactcccc ggtcgagcag
      841 tgcctggatc tgctcggcga tggccggatc ctcagggtcg aacgcgttga acctcacgtc
      901 tacgacgtcg ccttcgccga tacccggatt ggcgacgagc ggagcgaggt cggcgatatt
      961 gttgccatcc aggttcaggt tctcgagctc cgtgaacgcc tcgaggaacg ctatgtcgct
     1021 gatccccgtg gtggagagcg tcagggtggt caaagccgtc ttggcggtta gcgcgctcat
     1081 gtcttctatc acggctccac ggaggtcgag gaacaccaag tcgtccatga cgtcaaggaa
     1141 cgacaggttc gggatcgtgt gccagccgat gtcgagggcg tccagctggg ggtagtccaa
     1201 caggtggtcg aactcggtga ggtcgttgcc gggcagccag agcccatgga tgtccgggta
     1261 gttctcgagg agaggccaga tcagcgcgtc accgagcccg tggaacccca ggttcatggt
     1321 ccacatggag gtgagcgtcg caagcgccga cgtgtcggtg atcttggcct cggtgcagca
     1381 cacgtggagg ccccggatgt tcgttcggcc gcgcaggggc tcgatgtccg agatgtcgtt
     1441 gatgttgaga tcgacccacg tgaggctgtc cagcgtcgcg aggggagaga ggtcggtgat
     1501 ctcgccatcg gtcagttgga ggtcgtaaag gttcacggc
//
LOCUS       DAOQYE010000600         1537 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_101758_length_1537_cov_3.748313, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000600 DAOQYE010000000
VERSION     DAOQYE010000600.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101758_length_1537_cov_3.748313"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..843
                     /locus_tag="PKN52_09340"
     CDS             <1..843
                     /locus_tag="PKN52_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNR00195.1"
                     /translation="LSVVSALAGRHDILYYDRENHASLYDAARLSYATLRKYEHGDLA
                     DLERMLEADAGKPGGRIIVTDGVFSMSGHIADLPGIVKLKKRFGARLIVDDAHASGVL
                     GKHGRGTGEHFGLEREVDVIVGTFSKSFASVGGFMAGDRAVVNYVKHHARPFIFTAAL
                     PAMQMAAALEALNIMESEPEHLEKLWRNVRHLRDGMNALGFDTLGSQTPIVPVLIGPD
                     ELAMAFWKALWELDVFTTPALPPGVAPGRSIIRTSVNANHEPEHLDRLLHAFAEVGRR
                     FGVI"
     gene            850..>1537
                     /locus_tag="PKN52_09345"
     CDS             850..>1537
                     /locus_tag="PKN52_09345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00196.1"
                     /translation="MVDVERVNDRRAFREFVLYPYRKYAADRSWVAPLRVSQFELLDE
                     KKNPFFEHARATLYLAREGGRTVGRIALLDDDLHNETHVDDNLAFFGFFEAENAGVAS
                     ALFAAVEAEARSLGRRALRGPVNPTMNDGAGFQVDAFDELPYVMMPQSPAEYLTWATE
                     AGYSKIKDLYAFHLVNEGRVPDRVKRLADRARERYQPVIRTADMKRIHEEVKVLQRIH
                     HAAWEKNWGNV"
BASE COUNT          261 a          542 c          500 g          234 t
ORIGIN      
        1 ctctccgtcg tctccgcctt ggcgggcagg cacgacatcc tctactacga tcgcgagaac
       61 cacgcgtcgc tctacgacgc cgcccgcctc tcctacgcga ccttgcgcaa gtacgagcac
      121 ggcgacctgg cggacctgga gcgcatgctg gaagccgacg ccggcaagcc gggcggcagg
      181 atcatcgtca cggacggcgt attctcgatg agtggccaca tcgcggacct gccgggcatc
      241 gtgaagctca agaagcgctt cggcgcgcgg ctgatcgtcg acgacgccca cgccagcggc
      301 gtcctcggca agcacgggcg tggtaccggc gagcatttcg ggctcgagcg cgaggtcgac
      361 gtcatcgtcg gcacgttctc caagtcgttc gcgagcgtgg gcggcttcat ggccggggac
      421 cgcgcggtgg tcaactacgt caagcaccac gccagacctt tcatcttcac ggcggccctg
      481 ccggccatgc agatggccgc ggccctcgag gccctgaaca tcatggagtc cgagccggag
      541 catctcgaga agctctggag gaacgtgcgg cacctgcgcg acggcatgaa cgccctcggc
      601 ttcgacaccc tcggttcgca gaccccgatc gtacccgtcc tgatcgggcc ggacgagctg
      661 gccatggcgt tctggaaagc cctctgggag ctggacgtct tcacgacgcc cgcgctgccc
      721 ccaggggtgg cgccgggccg gtcgatcatc cgcaccagcg tcaacgccaa ccacgagccc
      781 gagcaccttg accggctcct ccacgcgttc gcggaggtcg gcaggcgttt cggcgtcatc
      841 tagccgcgca tggtcgacgt cgagcgcgtc aacgaccgcc gtgcgttccg agagttcgtc
      901 ctctacccgt accgcaagta cgccgccgac cggagttggg tcgcgcccct gcgcgtctcc
      961 cagttcgagc tcctcgacga gaagaagaac ccgttcttcg agcacgccag agccacgctc
     1021 tacctggcgc gcgagggtgg ccgcacggta gggcgcatcg ccctactcga cgacgacctc
     1081 cataacgaga cccacgtgga cgacaacttg gctttcttcg gcttcttcga ggcggagaac
     1141 gcgggcgtcg cctccgcact gttcgcggcg gtggaggcgg aggcgcgcag ccttggcagg
     1201 cgcgcgctcc gcgggccggt gaacccgacg atgaacgacg gcgccggctt ccaggtcgac
     1261 gcgttcgacg agctgcctta cgtgatgatg ccgcagagcc cggccgagta cctgacctgg
     1321 gccaccgagg ccggctatag caagatcaag gacctctacg cgttccacct ggtcaacgag
     1381 ggtcgggtgc ccgaccgcgt caagcgcctc gccgaccggg cgcgcgaacg ttaccagccc
     1441 gtgatccgca cggccgacat gaagcgcatc cacgaggagg tcaaggtcct ccagcgcatc
     1501 caccatgcgg cgtgggagaa gaactggggc aacgtcc
//
LOCUS       DAOQYE010000601         1537 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_101795_length_1537_cov_2.645074, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000601 DAOQYE010000000
VERSION     DAOQYE010000601.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101795_length_1537_cov_2.645074"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1438
                     /locus_tag="PKN52_09350"
     CDS             <1..1438
                     /locus_tag="PKN52_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00197.1"
                     /translation="ELFRQGMRRVGADTPTDDQLAELVAAVGRHPLAIELAAAWTRLL
                     APAELLERLERHPGFLANTPDGKPQPLTERFIDATRGLMSHAEQQALGTLALIPGGFT
                     AEDGRAASGASSFFLLALLERSLVRREGERYTVHAVVADRFRAGLDDEGAARDAVARA
                     YSEVARRLNELPSAERTARGFKQVDVERANLEWSFAWLASRSEPKAAWPLAELLRGYF
                     DVRGYYRQGAELFQRVTPALAACGDVELLGWALESVALYLMRQDELAKARDKITEAIA
                     LLEPSGRTRTLAMALNTAGIVASYEDRVDDALVHLQAAAELRAAMDDRFGEAQSRGNV
                     AFLLAGTERFADAFHALELAAGRYREVGHESGLALALTGMGRLASEHGLRQHGEALAW
                     VREAQALAERLGFAHGARVGATDAASILLRLGRPGEAVPALERACHWARIEENDAVER
                     SLREQLERARVQAARDQAGRDEREELLI"
BASE COUNT          223 a          533 c          572 g          209 t
ORIGIN      
        1 ggaactcttc aggcagggta tgcggcgcgt cggcgccgat acgccgacgg acgaccagct
       61 cgccgagttg gtcgccgccg taggacgcca cccgctcgcc atagagttgg cggcggcctg
      121 gacgcgcttg ctggctccgg cggagctgtt ggagcgcctg gagcggcacc cgggcttcct
      181 ggcgaacacc ccggatggga agccgcagcc gctcaccgag cggttcatcg acgccacccg
      241 ggggctcatg agccacgccg agcagcaggc cctcgggacc ctcgccctca tccctggcgg
      301 tttcacggcg gaggacgggc gggcggcctc cggcgcctcc tccttcttcc tgctcgcctt
      361 gctggagcgt tcgctcgtgc gccgtgaggg cgaacgttac acggtccacg cggtggtcgc
      421 cgaccggttc agagccggcc tcgatgacga gggggcggcc cgcgacgcgg tggcgcgcgc
      481 ctactccgag gtcgcgcggc gcctcaacga gctcccgagc gccgagcgca ccgcccgagg
      541 cttcaaacag gtggacgtcg agagggcgaa cctggagtgg tcgttcgcgt ggctggcctc
      601 gcgctcggag ccgaaagccg cgtggccgct ggctgaactc ctgcgcggct acttcgacgt
      661 gcggggttac taccggcagg gcgccgaact gttccagcgc gttacgcccg cgctcgcggc
      721 ctgcggcgac gtggagctcc tcggttgggc gctcgagtcc gtcgccctct acctgatgcg
      781 ccaagacgag ctggcgaagg caagggacaa gatcacggag gccatcgcgc tcctggagcc
      841 gagcggtcgc acgcgcacac tggcgatggc gctgaacacc gccggcatcg tggcgagcta
      901 cgaggaccgc gtcgacgacg cccttgtcca cttgcaggct gccgcggaac tgcgcgccgc
      961 catggacgac aggttcggcg aggcgcagtc gcgcggcaac gtcgccttcc tgctggctgg
     1021 aacagagcgt ttcgcggacg ccttccacgc cctcgaacta gcggccgggc gctaccgcga
     1081 ggtgggccac gagagcgggt tggcgttggc gctcactggc atggggaggc tggcgagcga
     1141 gcacggactg cgtcagcacg gggaggccct tgcttgggta agggaggcgc aggcgctggc
     1201 cgagcggctc ggcttcgccc acggcgcgcg cgtcggcgcc acggatgcgg cgagcatcct
     1261 cctgcgcctc ggccggccag gggaagccgt cccggcgctc gagcgagcat gccattgggc
     1321 gcgcatcgaa gagaacgatg ccgtcgagcg cagcctgcgt gaacagctcg agagggcgcg
     1381 cgtacaggcg gcgcgcgatc aggcggggcg cgacgagcgg gaagagctgc tgatctaggc
     1441 gccgaccgcc aagcgcgtgg cggctgccag cgcttcgagg acgtcggcca cgaggtcgtc
     1501 ggccgcctcc actccgaccg acaaccgcac caggccc
//
LOCUS       DAOQYE010000602         1536 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_101964_length_1536_cov_1.870358, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000602 DAOQYE010000000
VERSION     DAOQYE010000602.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101964_length_1536_cov_1.870358"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..733
                     /locus_tag="PKN52_09355"
     CDS             <1..733
                     /locus_tag="PKN52_09355"
                     /inference="COORDINATES: protein motif:HMM:NF014697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HNR00198.1"
                     /translation="PILNMMMLPKKKEDIIEVDPALFADDEEEIPVKARKDMTPAEKQ
                     ENSLILALFVVVIGAFYIVRFFVKGGSLNLDIVNIIFLILGLAMHRTPIAYVHAIDKS
                     IRNCGGIVLQFPFYAGIMGMMVKSGLAATFSNWFVSISTPQTFPFFTYLSAGLVNMFV
                     PSGGGQWAVQGPIMMPAAKALGVSYAQTASAIAWGDAWTNMLQPFWALPLLAVAKLNI
                     KDIMGYTTVILLWAGLITSLGLLLI"
     gene            874..>1536
                     /locus_tag="PKN52_09360"
     CDS             874..>1536
                     /locus_tag="PKN52_09360"
                     /inference="COORDINATES: protein motif:HMM:TIGR02429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HNR00199.1"
                     /translation="MVKKPIVTADEAVKLIKEGDAVMVGGFLGCGSPTGLIAALEKRG
                     TGKLTLICNDCAMYNPKTGDMNGVAPLVAKKRFSVAVVSHIGTNPEIQRQMNEGETEV
                     VLVPQGTLAERIRAAGSGLGGILTPTGVGTEVEEGKRKLLVKDKVYLLEEALGGDVAL
                     IKAAKADKAGNLRYSKTARNFNPLMATACALVIAEVEELVEIGALDPDDVHTPSIFVD
                     FIV"
BASE COUNT          290 a          520 c          469 g          257 t
ORIGIN      
        1 gccgatactc aacatgatga tgctgcccaa gaagaaggaa gacatcatcg aggtcgatcc
       61 cgcgctcttc gcggatgacg aagaggagat tccagtcaag gcgcgcaagg acatgacgcc
      121 ggccgagaag caggagaatt cgctcatcct cgcactcttc gtagtggtta taggcgcctt
      181 ctacatcgtg cgcttcttcg tcaagggcgg ctcgctgaac ctcgacattg tcaacatcat
      241 cttcttgata ttgggactgg ccatgcacag aacgccgatc gcgtatgtgc acgcgatcga
      301 caagtcgatc aggaactgcg gcggcatcgt gctccagttc ccgttctacg cgggcatcat
      361 gggcatgatg gtcaagtccg gcctcgccgc gacgttctcc aactggttcg tcagcatctc
      421 gaccccgcag accttcccgt tcttcaccta cctgtccgcg ggcctcgtca acatgttcgt
      481 gccgtccggc ggcggccagt gggccgtcca gggcccgatc atgatgccgg ccgccaaggc
      541 cctcggcgtc tcgtacgccc agaccgcctc cgcgatcgcg tggggcgacg cgtggaccaa
      601 catgctccag ccgttctggg ccctgccgct gctcgcggtc gccaagctga acatcaagga
      661 catcatgggc tacaccacgg tgatactgct gtgggccggc ctcatcacgt ccctcggact
      721 gctcttgatc tagactagat cgccttacgg gcgctccggc ccaggccggc cggcctgagc
      781 cggagcgccc gattccccga agcctaccat cagacacacg cctcgctacg cgcgcctggg
      841 acgggctcat tccgatcgaa aaggagcgac gatatggtaa agaaacccat cgtcacggcc
      901 gacgaggccg tgaaactcat caaggagggc gacgccgtca tggtcggcgg cttcctcggc
      961 tgcggctcgc cgaccggcct gatcgccgcg ctcgagaagc gcggtaccgg taagctgacc
     1021 ctgatctgca acgactgcgc catgtacaac cccaagaccg gcgacatgaa cggcgtggcc
     1081 ccgctcgtcg ccaagaagcg cttcagcgtc gccgtcgtct cgcacatcgg caccaacccc
     1141 gagatccagc ggcagatgaa cgagggcgag accgaggtgg tgctggtgcc gcagggcacc
     1201 ctggccgagc ggatccgcgc ggcgggctcg gggctcggcg gcatcctgac tccgaccggt
     1261 gtcggcaccg aggtggagga aggcaagcgg aagctcctcg tcaaggacaa ggtgtacctg
     1321 ctcgaggaag ccctgggcgg cgacgtggcc ctgatcaagg cggccaaggc cgacaaggcg
     1381 ggcaacctgc gctactcgaa gaccgcgcgg aacttcaacc ccctcatggc cacggcctgc
     1441 gccctcgtca tcgccgaagt ggaggagctc gtcgagatcg gcgcgctcga ccccgacgac
     1501 gtgcacacgc cgtccatctt cgtcgacttc atcgtc
//
LOCUS       DAOQYE010000603         1535 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_102010_length_1535_cov_2.899324, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000603 DAOQYE010000000
VERSION     DAOQYE010000603.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102010_length_1535_cov_2.899324"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..860)
                     /locus_tag="PKN52_09365"
     CDS             complement(<1..860)
                     /locus_tag="PKN52_09365"
                     /inference="COORDINATES: protein motif:HMM:NF014712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyltransferase domain-containing protein"
                     /protein_id="HNR00200.1"
                     /translation="MHLETVGPGASYLVFDDPPSPELSRRIGAVAEALRSALPRGAFD
                     VVPGYQDILVEGRTALSRARLLALAQEALDAPAARRLPRAFEIPVAYGAKADAAALAD
                     RLGMAWADIAALHAAGKYTVAYVGFTPGFPYLHGLDQRLRTPRRDRPRPDVPAGAVAM
                     AGAQAGIYPHAGPGGWWVLGTTPFPLFMPERAEPTVLRAGDEVRFVAVEEGRGAPATL
                     DQPVYPGGTDLPHVAGVPARERPAGADRLVEPDRADRAVEPDPLVTVLEVWPGSASLQ
                     GRPRVGVGHYG"
     gene            complement(872..>1535)
                     /locus_tag="PKN52_09370"
     CDS             complement(872..>1535)
                     /locus_tag="PKN52_09370"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF003814.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HNR00201.1"
                     /translation="NVACGFHAGDPTIMRETVGLCAAAGVTVGAHPSYPDLRGFGRVP
                     LMLPPERVIDDVLYQVGALVGIAAAQGVAVKHVKPHGALYEAVAVDPGLARALATALT
                     LVSDGLRLVVPASSAAAAALASGGHPHLLEGFADRAYLESGLLVPRRRSGAVLTDAGL
                     AADQALALAQGREFSTIDGTAIALTVDTICVHSDTPGAVTIAAAVRDRLVGAGFTVAA
                     PA"
BASE COUNT          215 a          559 c          588 g          173 t
ORIGIN      
        1 ccgtagtggc cgaccccgac acgcggccgg ccctggagcg acgccgaccc gggccacact
       61 tcgagcacgg tgacgagggg gtcgggctcg acggcacggt cggcacggtc gggctcgacc
      121 agccggtcgg cgccggcggg ccgttctcgt gccgggactc ccgcgacgtg cggcaggtcc
      181 gtgccgcccg gatagaccgg ctggtcgagc gtggccggcg cgccgcggcc ctcctcaacg
      241 gccacgaacc gcacctcgtc acccgcgcgc aacaccgtcg gctccgcccg ttcgggcatg
      301 aagagcggga agggcgtcgt accgagcacc caccaccccc cgggtcccgc atgagggtag
      361 atgccggcct gggcacccgc catcgccacc gcgcccgcgg gcacgtcggg tcgcgggcgg
      421 tcgcgcctgg gcgtccgtag gcgctggtca agaccgtgca ggtaggggaa gcccggcgtg
      481 aagccgacgt aggcgaccgt gtacttcccg gcggcgtgca gcgccgcgat gtcggcccag
      541 gccatgccga ggcggtccgc cagcgccgcg gcgtccgctt tcgccccgta ggcgaccggg
      601 atctcgaagg cgcgtggcaa gcgccgggcg gcgggggcgt ccagcgcttc ttgcgccagc
      661 gctaggagac gggctcgcga gagcgccgtc ctgccctcga ccaagatgtc ctgataaccg
      721 ggcaccacgt cgaaagctcc gcgcggcagg gccgagcgca gcgcctcggc cacggcgccg
      781 atgcgcctcg agagctccgg agagggcggg tcgtcgaaga cgaggtagct ggcgcccggc
      841 ccgacggtct ccaggtgcat gcctgcgccg gtcaggctgg ggctgccacg gtgaacccgg
      901 cgccgacgag ccggtcgcgc acggcggcgg cgatggtgac cgcgccgggc gtgtcggagt
      961 gcacgcagat cgtgtcgacc gttagcgcga tggccgtccc gtcgatggtg gagaactccc
     1021 ggccctgcgc caaggcgagc gcctggtccg ccgccaagcc ggcatccgtc agcacggcgc
     1081 cgctgcgccg ccgcggcact aggaggccgc tttcgaggta agcccgatcg gcgaagcctt
     1141 cgaggaggtg cgggtggccg ccggaggcga gcgcggccgc cgcggcggac gaggccggca
     1201 cgacgaggcg caacccgtca ctcaccaacg tgagggccgt ggccagagcc cgcgcgaggc
     1261 ccgggtcgac cgcgacggcc tcgtagaggg cgccatgcgg cttgacatgc ttgacagcca
     1321 cgccctgggc ggcggcgatc cccacgagcg cgccgacttg gtaaaggacg tcgtctatca
     1381 cgcgctcggg cgggagcatg agcggcacgc ggccgaagcc ccgcaggtcc gggtacgagg
     1441 ggtgcgcccc gacggtcacc ccggccgcgg cgcacaagcc gacggtctca cgcatgatcg
     1501 tcgggtcgcc cgcgtggaag ccgcaggcca cgttg
//
LOCUS       DAOQYE010000604         1534 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_102078_length_1534_cov_4.010142, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000604 DAOQYE010000000
VERSION     DAOQYE010000604.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102078_length_1534_cov_4.010142"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="PKN52_09375"
     CDS             complement(<1..417)
                     /locus_tag="PKN52_09375"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNR00202.1"
                     /translation="MRLTCAGAARTVTGSCHHLDLGDFRLVVDCGLFQGGAEFDALNR
                     APFPFEPQDVNAVLVTHGHLDHVGRLPLLVKAGYQGRFLATKATADVAAIILRDAAKL
                     QEEDFERDLRKARRAGRESELPGPLYTLADVEAALTR"
     gene            complement(414..1277)
                     /locus_tag="PKN52_09380"
     CDS             complement(414..1277)
                     /locus_tag="PKN52_09380"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNR00203.1"
                     /translation="MNCAIVDVGDGRALLVDSGQDKEYGRKVRKALDALGLELTLIVA
                     THSHADHYGGNAYLLRQFPEARVWAPEIEADVIRTPALEPIYLFHGAKPLPELTSKWL
                     QADASPVHRVVAAGDELCGAVGFRLLDVRGHAHRQLAVLVDDVLLAADAVFGAETLEK
                     YPIPFAQDVAGQLAAFETVAGVSARVLLPGHGAPTEDLAGVAESNRRAVLRAADAILA
                     ACAAPGARPGTSMEDVVAGSAETLGVMLDDLARYHLNYCVVSAHLGRLRELGLVACGV
                     EARRLVWRRTA"
BASE COUNT          213 a          544 c          574 g          203 t
ORIGIN      
        1 gcgcgtgagc gccgcctcga cgtcggccag cgtgtagagc ggccccggca gctcggactc
       61 gcggccggcg cggcgggcct tgcggaggtc gcgctcgaag tcctcctcct gcagcttggc
      121 ggcgtcgcgg aggatgatgg ccgcgacatc ggccgtcgcc ttggtcgcca ggaagcgtcc
      181 ctggtagccc gccttgacga gcaggggcag gcgcccgacg tggtcgaggt gcccgtgggt
      241 cacgaggacg gcgttgacgt cctgcggctc gaaagggaag ggggcgcggt tgagcgcgtc
      301 gaactcggcg ccgccctgga agaggccgca gtcgaccacg agccggaagt cgccgaggtc
      361 gaggtggtgg cagctgcccg tcaccgtgcg ggccgcgccg gcgcaggtca gcctcatgcc
      421 gtccgcctcc agacgaggcg gcgggcctcg accccgcacg cgaccagccc gagctcccgc
      481 agcctgccga ggtgggccga cacgacgcag tagttgaggt ggtagcgcgc caggtcgtcc
      541 agcatcacgc cgagcgtctc ggcggagccg gcgacgacgt cctccatgct cgtgccgggg
      601 cgcgcgcccg gcgcggcgca ggccgccagg atggcgtcgg ccgcgcgcaa cacggcgcgg
      661 cggttgctct cggccacgcc cgcaaggtcc tcggtcggag cgccgtgccc aggaaggagg
      721 acgcgggcgc tcacgccggc gaccgtctcg aaagccgcaa gctggcccgc cacgtcctgc
      781 gcgaacggga ttgggtactt ctccaacgtc tcagcaccga acacggcgtc ggccgctagc
      841 agcacgtcgt cgacgagcac ggcgagctgc cggtgggcgt ggccgcgcac gtcgagcagc
      901 cggaaaccga cggccccgca caactcgtcg ccggcggcca ccacgcggtg gacgggggag
      961 gcatcggctt gcagccactt gctcgtgagc tccgggagag gcttggcgcc gtggaagagg
     1021 tagatgggct cgagcgcggg agtgcggatg acgtcggcct cgatctccgg ggcccagacc
     1081 cgggcctcgg ggaactgcct gaggaggtag gcgttgccgc cgtaatggtc ggcgtgcgag
     1141 tgggtggcga cgatgagggt cagctccaac ccgagcgcgt cgagcgcctt acggaccttg
     1201 cgcccgtact ccttgtcctg gccgctgtcg accagcagcg cgcgaccgtc tccgacatcg
     1261 acgatcgcgc agttcacgcc gccgaggacc gcgtggacgc gctcggacag ctgggccgac
     1321 gtcctcttct cttcgagcgg ggctccgttc tcttcgaccg gggctccggc tacggccccg
     1381 gaggccgccc ctggcgttgc gccgccgcag cgctcgcctt cagccggacg ctcccagcta
     1441 ggcgctccta cgcaagcacg ccacgtagaa gccgtcgagc ccgccgagcg gaagcacgta
     1501 ctggcccggg ccagtacgtg cttccgctcg gcgg
//
LOCUS       DAOQYE010000605         1534 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_102094_length_1534_cov_3.150778, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000605 DAOQYE010000000
VERSION     DAOQYE010000605.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102094_length_1534_cov_3.150778"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1291)
                     /locus_tag="PKN52_09385"
     CDS             complement(<1..1291)
                     /locus_tag="PKN52_09385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00204.1"
                     /translation="MIRRQLSLATVVLAVALWSGAAFAQEDSLLQRYDLALENLQIAT
                     ASVPGDGLQARDELERALNALLTLSRDATSSNLVQAMERTFERTRVAVENQSHTDMAV
                     QTTVLAGGFSRLVLDSAFSSASSGDVALARDRLDHLARTLDFGADAISEITNADSAAT
                     LRLAFEAGAADAVAAELEVATQLVDEDRDGAYVSLARAYGSSLLVQDSPRVDATLNRD
                     LVSAAQALVAGDRTELDAATERATTSLTRLAAAARNAAADPGASATVIAVTESVGETT
                     TEPATLLADVLPEVGATAQAAPSDEPTTNEATTETTPEADQPAADEPSGDALSSDVLS
                     SAALDEAVAARLAELAAEEHQARIDGVSLDLVRAGVPRALAGSQAERVVGAGFPDLAA
                     VFADLDAGAGRMLAGVRRGAQDEAHAALAGFTATYDSA"
     gene            complement(1288..>1534)
                     /locus_tag="PKN52_09390"
     CDS             complement(1288..>1534)
                     /locus_tag="PKN52_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00205.1"
                     /translation="RRNAILLQRGEGVDAERLPGLVLSSRHLLARSSDLRALSVEHPA
                     GVLFLFPFGEDSLVVVTQGAVNIGAVLNARAALEEAA"
BASE COUNT          220 a          503 c          588 g          223 t
ORIGIN      
        1 cggcgctgtc gtaggtcgcg gtgaagccgg cgagagcggc atgggcctcg tcctgcgctc
       61 cgcggcgcac gcccgcgagc atgcggcccg cgccggcgtc gagatctgcg aacacggcgg
      121 ccaagtcggg gaagcccgca ccgactaccc gctcggcctg cgagcctgcc agcgcccgcg
      181 ggacgcccgc cctgacgagg tcgaggctca cgccgtcgat cctggcctgg tgctcttcgg
      241 cggccagttc ggccagtctc gcggcgacgg cctcatcgag cgcagcggat gagaggacat
      301 ccgatgagag cgcgtcaccg gagggctcgt cggcggccgg ctggtccgct tccggggtcg
      361 tctcggtcgt ggcctcgttg gtggtcggtt catccgaggg ggccgcctgg gccgtcgcac
      421 cgacttccgg cagcacgtcc gccaagaggg tggcgggctc cgttgtcgtc tcaccgacag
      481 actcggtgac cgcgatgacg gtggcggagg ctccggggtc ggcagcggcg ttgcgcgcgg
      541 cggcggccag tcgcgtgagc gaggtggtcg cgcgttcggt cgcggcgtcg agctcggtgc
      601 ggtcgccggc gaccagcgct tgagccgcgc tcaccaggtc gcggttgagc gtcgcgtcaa
      661 cgcgcggcga gtcctggacg agtaacgagc tgccgtaggc cctggcgagg ctgacgtagg
      721 cgccgtcgcg gtcctcgtcg acgagttgcg tcgcgacctc gagttccgca gctacggcat
      781 cggcggcgcc ggcctcgaag gcgagccgca acgtcgcggc ggagtcggcg ttcgttatct
      841 ccgagatggc gtccgcgccg aagtcgaggg tccgcgcgag gtgatcgaga cggtcgcggg
      901 ctagcgccac gtcgccgctg ctggcgctcg agaacgccga gtcgaggacg aggcgactaa
      961 aaccgcccgc caacacggtc gtctggaccg ccatgtcggt gtggctctgg ttctcgacgg
     1021 ccacgcgcgt gcgttcgaaa gtgcgctcca tggcctgcac gaggttcgag ctggtggcgt
     1081 cacgcgacag ggtcagcagg gcgttcaggg cgcgttccag ctcgtcgcgc gcttggaggc
     1141 cgtcgcccgg caccgaggcg gtggcgatct gcaggttctc gagggcgagg tcgtaccgtt
     1201 ggaggaggga gtcctcctgc gcgaacgcgg ctcccgacca gagcgccacg gccaacacca
     1261 cggtggcgag agagagttga cggcggatca tgcagcctcc tcgagagcgg cgcgcgcgtt
     1321 caggacggca ccgatgttga cggctccttg ggtgacgacg acgaggctgt cctcaccgaa
     1381 agggaacagg aagagcacac cggcggggtg ctcgaccgac agggcgcgca agtcgctcga
     1441 gcgcgccaga aggtgacgag aagaaagtac cagacccggg agacgctccg cgtccacgcc
     1501 ctccccgcgt tggaggagga tggcgttgcg cctg
//
LOCUS       DAOQYE010000606         1532 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_102331_length_1532_cov_2.839540, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000606 DAOQYE010000000
VERSION     DAOQYE010000606.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102331_length_1532_cov_2.839540"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            377..>937
                     /locus_tag="PKN52_09395"
     CDS             377..>937
                     /locus_tag="PKN52_09395"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNR00206.1"
                     /translation="METNAAIETSVVSEGAAKAKVVEATTGTVFTVIAAISFSHFLND
                     LMQALLPAIYPMLKDDHALTFTQVGLLTLTFQFTASLLQPVVGIVADRRPLPYSMAVG
                     MGSTLAGLLLLARATSFPFLVLAAALVGLGSSVFHPEASRVARMAAGMRPGLAQSLFQ
                     VGGNFGSALGPLLAAFIVLPFGQGSIA"
     assembly_gap    938..947
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <948..>1532
                     /locus_tag="PKN52_09400"
     CDS             <948..>1532
                     /locus_tag="PKN52_09400"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNR00207.1"
                     /translation="FSIVAVLAMVVLFRVGKWYASHVGLLYGKSRKVVAVASQKRIVA
                     AVLILIGLTFSKAVYGSSLNSYYTFFLIERFGVSIRDSQLLLFVYMVAVVIGTIIGGP
                     LGDRFGRKIVLWGSVLGALPFTLLLPWVNLTWTLILTALIGIIMASAFPAIVVYAQEL
                     LPRGVGMVAGLLYGLSFGVAGVAGVSFPQAAAAH"
BASE COUNT          209 a          538 c          477 g          298 t
ORIGIN      
        1 cgacggattc gatcttgaag tccttgtgat ccgcggtctg gcgcaggcgc cgttcggcaa
       61 aggcgcgccg ctccccgatc ttcacagcct gatgtgacgg gccgatgagg aacatcgcct
      121 tgccgagctt ctcgtggcct tggagcatcg cgtggtacca gtcctgtcgg ccacggggag
      181 ccgcggcgcc tgaatcccta gtatgttact aggctatagc tgtcggttca acgctcgggc
      241 cccacgcctg gtcacgcgag cttggcgttc ccgccacttc ccgagcccga aagcggtaca
      301 ttctcggggt cttgctagac gcgcccaagg cgcgccgccc gggtcggagc tgagcgagtt
      361 gtcgagcgac gccacagtgg aaaccaacgc cgcgatcgaa acgagtgtcg tatcggaggg
      421 cgctgcgaag gccaaggtcg tcgaagcgac gaccggcacc gtcttcaccg tcatagccgc
      481 catcagcttc tcgcacttcc tcaacgacct catgcaggcg ctgctgcctg ccatctaccc
      541 gatgctaaag gacgaccacg cgctcacgtt cacccaggtc ggcctgctca cgctgacgtt
      601 ccagttcacg gcctcgctgc tccaaccggt ggtcggcatc gtcgccgacc ggcgtccgtt
      661 gccttactcg atggcggtcg gcatgggctc gaccctggcc ggcctgctgc tgctcgcgag
      721 ggcgaccagc ttccccttcc tcgtgctggc cgccgcgctc gtagggctcg gctcctccgt
      781 gttccacccc gaggcctcga gggtcgcccg catggcggcc ggcatgcggc ccggtctagc
      841 ccaatccctg ttccaggtcg gtgggaactt cggctctgcc ctcggtccac tcctggccgc
      901 cttcatcgtc ctgcctttcg gccaggggag catcgccnnn nnnnnnnggt tctccatcgt
      961 ggccgtgctg gccatggtcg tgctcttcag ggtggggaag tggtacgcga gccacgtcgg
     1021 cctgctgtac ggcaagagca ggaaggtcgt cgccgtggcg agccagaagc gcatcgtggc
     1081 cgccgtcctc atcctcatcg gcctgacgtt ctcaaaagcc gtgtacggct cgagcctcaa
     1141 cagctactac acgttcttcc tgatcgagcg cttcggggtc tccatccgcg actcgcagct
     1201 cctcctgttc gtctacatgg tcgccgtcgt catcggcacc atcatcggcg ggccgctcgg
     1261 cgaccgcttc gggcgcaaga tcgtcctgtg ggggagcgtc ctcggcgccc tgccgttcac
     1321 cctcctgctc ccatgggtga acctgacctg gacgctcatc ctgacggcgc tcatcggcat
     1381 catcatggcc tcggccttcc cggccatcgt cgtgtacgcg caggaactcc tgccgagggg
     1441 ggtcggcatg gtagccgggc tcctgtacgg actctcgttc ggcgtggccg gcgtcgcggg
     1501 cgtgtcgttc ccgcaggcgg cggccgcgca cg
//
LOCUS       DAOQYE010000607         1531 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_102473_length_1531_cov_2.315041, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000607 DAOQYE010000000
VERSION     DAOQYE010000607.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102473_length_1531_cov_2.315041"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="PKN52_09405"
     CDS             <1..496
                     /locus_tag="PKN52_09405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00208.1"
                     /translation="ASRVTVVAGSVPEAFDELPDVVAGFVCAHMLGHVGPADRARTFA
                     LLAERLADGGVGAVTVNATAEPRSEVAVEERRIGEHSYIAKYLPSGPDGRPSSEYEVR
                     DADGSLLRAERFAGQWTPITAEGLRAELVNDGWRLTAQDRAATVLVLSREREDDSLAS
                     SQAR"
     gene            complement(447..>1531)
                     /locus_tag="PKN52_09410"
     CDS             complement(447..>1531)
                     /locus_tag="PKN52_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00209.1"
                     /translation="DGAFYSALDAESEGEEGKYYVWTAAELSDVLGGEAARLVPVAGR
                     VDEGLVLAGGLAADARARLLDRRSLRPRPATDDKVLASWNGLAIGALADASLALAEPR
                     LAAVSARAAAAVRERLWSDGRLWHSSCAGQRRVEGLLEDYAYLGLGLIALHRATCDWR
                     HLAWALELAAAVASRFADRAGDGFFSVAEGDRGLIARPKGFLDGATPSENAAAAELTW
                     WAARYQDDPAAAARSERAVRGAAAAAGEAPQALASSARLAWLQASGQREVVIVGASGS
                     ALADLSSALEALVDPGLLVLAVDPGAPAELLRLPLLAGRLGALSGGAPRAYVCQAGAC
                     RLPVSTSDDLASELASSLANRPPARG"
BASE COUNT          235 a          559 c          555 g          182 t
ORIGIN      
        1 cgctagcaga gtcacggtcg tagccggctc cgtgcccgag gcgttcgacg agctgccgga
       61 cgtggtggct gggttcgtgt gcgcgcacat gctcggccat gtcggccccg cggaccgcgc
      121 caggaccttc gccttgctgg ccgagcgcct cgcggacggc ggggtcgggg ccgtcaccgt
      181 caacgcgacg gcggagccac ggagcgaagt agccgtcgag gagcgtcgga tcggcgagca
      241 ctcgtacatc gcgaagtacc tgccgtcggg accggacggt cggcccagca gcgagtacga
      301 ggtgcgagac gcggacgggt ccctgctgcg ggcggaacgc ttcgccgggc agtggacgcc
      361 catcacggcg gaagggttga gggccgaact cgtcaatgac gggtggcgcc tgactgccca
      421 ggaccgagct gcgactgtgc tcgtgctcag ccgcgagcgg gaggacgatt cgctagcgag
      481 ctcgcaagct cgctagccaa gtcgtccgat gtgctcaccg gcaaccggca ggcgccggct
      541 tggcacacgt aggcccgcgg cgcgccgccg gagagcgcgc cgaggcgccc tgccaggagc
      601 ggtaggcgca ggagttccgc gggcgcgcca gggtctactg ccaggaccag caacccggga
      661 tcgacgagcg cttccaaggc gctactgagg tccgccagcg cggagccgga cgcaccaacg
      721 atcacgactt cgcgctgccc gctagcctga agccacgcga gccgtgcgct ggacgcgagg
      781 gcctggggcg cctcgcccgc agcggcggcg gccccgcgaa cggctcgctc cgagcgcgcc
      841 gcggccgccg gatcgtcctg gtagcgggcc gcccaccacg tcaactcggc ggcggcggcg
      901 ttctcgctcg gcgtggcgcc gtcgaggaac cccttcggtc gggcgatcag accccgatcg
      961 ccctccgcca cgctgaagaa gccgtcgccg gcccgatcgg cgaaacgact cgcgacggcg
     1021 gcggccagct cgagggccca cgccaggtgt cgccagtcgc aggtggcgcg gtgcagggcg
     1081 atcagtccaa ggcccaggta ggcgtagtcc tcgagcaggc cctcgacacg gcgttggccg
     1141 gcgcacgagg agtgccacag acgcccgtcc gaccacaggc gctctcgcac cgcggcggcc
     1201 gccctggcgg agaccgccgc cagacggggc tcggccaggg caagtgaggc atcggccaac
     1261 gcaccgatgg ccaagccgtt ccacgacgcg aggaccttgt cgtcggtggc gggcctgggg
     1321 cgaagggacc tgcggtcaag gagcctcgcg cgcgcgtcgg cagccaagcc gcccgccagc
     1381 acgaggccct catccacccg accggccacc ggcactagac gagcggcctc gcccccgagc
     1441 acatcgctca actcagcggc ggtccatacg tagtacttcc cctcctcccc ttccgactcg
     1501 gcatccagcg cgctgtagaa ggcgccgtcc c
//
LOCUS       DAOQYE010000608         1530 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_102570_length_1530_cov_2.499661, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000608 DAOQYE010000000
VERSION     DAOQYE010000608.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102570_length_1530_cov_2.499661"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="PKN52_09415"
     CDS             complement(<1..656)
                     /locus_tag="PKN52_09415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00210.1"
                     /translation="MAAGYIQAALEQVPNSENGSNKVSTTVFYLPGTSIEIDPDMTVL
                     ERGDELRGGFYQAPHAGAAEYAPKHTINSRMYPAVMGLYLCLLCGGVTTTEGDGGSVK
                     DPAGVSIPASAYRHVFSWKTGDVPQTAQLIACAPDGGAFWKLQGVGANQMTFSRDGGA
                     WVCEGELLSLVAKTISDPTLTPAYEAAKPWRAGQMTLSWLGSTAVTEDFEWKIDNGLT
                     T"
     gene            complement(656..898)
                     /locus_tag="PKN52_09420"
     CDS             complement(656..898)
                     /locus_tag="PKN52_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00211.1"
                     /translation="MKAVRSSGPPTEPREFPDFPGLYRSGVSLPLSELGLSEEEAAAR
                     LPDGLEIVDLPGLESAAAGRQEPGRSKDARVKGGED"
     gene            complement(912..1406)
                     /locus_tag="PKN52_09425"
     CDS             complement(912..1406)
                     /locus_tag="PKN52_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00212.1"
                     /translation="MSWQGMEPVVSAVIEALQDGLAGAAAAINAGLPDTIRIRAPHES
                     AYLLGIDPGELESETLQLADPSVIVRGEDTTPKPDSGNPALGGEYALDNAVVVSVLFQ
                     APTLEERVKLTWRYQRAVCDVLLPDPDLGATFLGSGYLERTIPSGVWRDAVCLFNVDS
                     FETP"
BASE COUNT          209 a          547 c          530 g          244 t
ORIGIN      
        1 gtcgtgagcc cgttgtcgat cttccactcg aagtcctcgg tcaccgccgt gctgcccagc
       61 caggagagcg tcatctggcc agcgcgccag ggcttggcgg cctcgtaggc gggcgtcaac
      121 gtggggtcgg agatcgtctt cgccaccagg ctgagcagct cgccctcgca gacccaggcg
      181 ccgccgtcac gcgagaaggt catctggttg gcgcccacgc cctgcagctt ccagaaggcg
      241 ccgccgtccg gagcgcaggc gatcagttgg gcggtctgcg gcacgtcacc ggtcttccag
      301 ctgaacacgt gccggtaggc gctggccggg atcgagacgc cggccgggtc cttgaccgag
      361 ccgccgtcgc cttccgtggt agtgacaccc ccgcagagca ggcagaggta gagccccatc
      421 acggccgggt acatgcgact gttgatggtg tgcttgggcg cgtactcggc cgccccggca
      481 tggggcgcct ggtagaagcc gccgcgcagc tcgtcgccgc gctccagcac ggtcatgtcg
      541 gggtcgatct cgatgctcgt gccgggcagg tagaacaccg tcgtactgac cttgttggag
      601 ccgttctcgg agttcggcac ctgctccagg gccgcctgga tataacccgc agccatcagt
      661 cctcaccgcc cttcacccgg gcgtccttgg accggcccgg ttcctgtcgc cccgccgcgg
      721 ccgactccag ccccggcagg tcgacgatct cgaggccgtc gggcaggcgc gccgcagcct
      781 cctcctcact cagtcccagc tcggacagcg gcagactgac gccggagcgg tagagccctg
      841 ggaagtccgg gaactcgcgc ggctccgtcg gcgggccgct gcttctcacg gccttcatcg
      901 gggcgctcct ttcatggggt ctcgaacgag tcgacgttga acaggcagac ggcgtcgcgc
      961 cagacgccgc tcgggatggt gcgctccagg tatcccgagc ccaggaaggt ggcgccgagg
     1021 tcgggatcgg gcagcagcac gtcgcagacg gcgcgctggt aacgccaggt cagcttgacc
     1081 cgctcctcca gggtgggcgc ctggaagagc acgctcacca cgacggcgtt gtcgagcgca
     1141 tactcgccgc cgagcgccgg gttgccggag tcgggcttgg gcgtcgtgtc ctcaccgcgc
     1201 acgatcacgc tcgggtcggc cagctgcagg gtctcactct cgagctcgcc cgggtcgatg
     1261 ccgagcaggt aggcggactc gtgcggcgcc ctgatcctga tcgtgtcggg caggccggcg
     1321 ttgatcgccg cggccgcgcc ggcgaggccg tcctggagcg cctcgatcac ggcgctgacg
     1381 accggctcca tgccctgcca gctcacggca cgcctgccag cacggagtgg gtcgcgacgc
     1441 cgccgccgag ccggatcgcc tcgggcaggc tctcgttgcc gacacctgcc ttgcgcgcct
     1501 cggcgatgac ccactgcttg gtcagctcga
//
LOCUS       DAOQYE010000609         1530 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_102606_length_1530_cov_1.879322, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000609 DAOQYE010000000
VERSION     DAOQYE010000609.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102606_length_1530_cov_1.879322"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..673)
                     /locus_tag="PKN52_09430"
     CDS             complement(<1..673)
                     /locus_tag="PKN52_09430"
                     /inference="COORDINATES: protein motif:HMM:NF037997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015114 - phosphate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi symporter"
                     /protein_id="HNR00213.1"
                     /translation="MLLTLLGGLALFLTGIYRISGALQDMLGPASRRWMAFATRSPLV
                     ALLMGTGVAAATQSATATAITILGLVGGGLVAVKEGIALLLGAKLGATLAMQLAAFHL
                     SDYALPMVGVGYVLAMWRRAAAVGGFLFGAGLLFFGLDITVQSVSGLSSSDAFNVLVA
                     AAEQQPLAVLVLGAALGMMLSSSNAATAVALGLHVAGAISLETAVALVVGGNAGSAFM
                     PLMVSR"
     gene            915..>1530
                     /locus_tag="PKN52_09435"
     CDS             915..>1530
                     /locus_tag="PKN52_09435"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNR00214.1"
                     /translation="MKRALVVAFVAALALTAGAALAQDTVKIGVITSITGRFAEFGEQ
                     HQAGIKAALEDVNAAGGVNGRAVEVVYEDDTSEVNAALTAGEKLVNQGLPLVMGAYSS
                     SITGPLAQYFTRQKRPFVVYTSADDAITKPGSDWVFRINQPSFAYATTLFDVFDQINA
                     ERGAGTLKTVVTIHGNGAFETSVADAVDEMAKARGYTVLQRQDYD"
BASE COUNT          258 a          537 c          550 g          185 t
ORIGIN      
        1 tgcgcgacac catgagcggc atgaacgccg agccggcgtt gccgccgacg acgagcgcca
       61 cggccgtctc gagggagatg gccccggcga cgtgcaggcc gagggccacg gccgtggcgg
      121 cgttcgagct cgacagcatc atgccgagcg ccgcgccgag cacgagcacg gcgagcggct
      181 gctgctccgc ggccgccacc agcacgttga aggcgtcgct gctgctgagc ccggacaccg
      241 actgcaccgt gatgtcgagg ccgaagaaga gcaggccggc gccgaacagg aagccgccga
      301 cggcggcggc gcgacgccac atggccagga cgtagccgac gcctaccatg ggcagcgcgt
      361 agtcggagag gtggaaggcg gcgagctgca tggcgagcgt ggcgccgagc ttcgcgccga
      421 ggaggagggc gatcccctcc ttcacggcca ccagcccgcc cccgacgagg ccgaggatgg
      481 tgatggcggt ggcggtggcg ctctgcgtgg cggccgccac gccggtgccc atgagcagcg
      541 ccacgagggg ggagcgcgtc gcgaacgcca tccagcgccg cgacgccggg ccgagcatgt
      601 cctgcagcgc gccggagatc cggtatatgc ccgtcaggaa cagcgcgagc ccgccaagca
      661 gggtcagcag cacggagacc ccccgcctcg ccggcgcggg gcgaccggcc gggtccgaag
      721 tggcggtggt gtgggtgagg ctggctgcaa cacgctgggt ccggtcctac ggatgaatac
      781 ggagtctacc cctccgggca ccgcgcaagc tcaccggccc cgccgatttg tacgcccaac
      841 cgtccctgcg cgcccgccga ccgccgctca ccctggtaac ttgccggtga cagcacagca
      901 gggaggggcg aagcatgaag cgagcactcg tcgtcgcgtt cgttgcggcg ctggccttga
      961 cggccggcgc ggcgttggcg caggacaccg tcaagatcgg ggtgatcacg agcatcaccg
     1021 gccggttcgc ggagttcggg gagcagcacc aggccggcat caaggcggcg ctggaggacg
     1081 tgaacgccgc cgggggcgtg aacgggcgcg ccgtcgaggt cgtctacgag gacgacacct
     1141 ccgaggtcaa cgccgccctc acggccggcg agaagctcgt gaaccagggg ctgccgctcg
     1201 tcatgggggc gtactcctcc tccatcacgg gtccgcttgc gcagtacttc acgcgccaga
     1261 agcgaccgtt cgtcgtctac acgtcggcag acgacgccat caccaagccc ggctccgact
     1321 gggtgttccg catcaaccag ccgtcgttcg cctacgcgac caccctgttc gacgtgttcg
     1381 accagataaa cgccgagcgc ggcgccggca ccctcaagac ggtcgtgacc atccacggca
     1441 acggcgcctt cgagacctcc gtcgcggacg ccgtcgacga gatggccaag gcgcgcggct
     1501 acacggtgct gcagcgtcag gactacgacc
//
LOCUS       DAOQYE010000610         1525 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_103112_length_1525_cov_3.400680, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000610 DAOQYE010000000
VERSION     DAOQYE010000610.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103112_length_1525_cov_3.400680"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..746)
                     /locus_tag="PKN52_09440"
     CDS             complement(<1..746)
                     /locus_tag="PKN52_09440"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HNR00215.1"
                     /translation="MPTFGTRGDVPDALVRSFMPAFRSAVGAATGLEVRNGELITPGI
                     AGSLEPEFAMLIAELDQTRYAVSGEVARSSASGDDVYAVNMIVVDSERDRATDLISLP
                     LDPDDQRTAIAQLAAAVATFTSAIVDLQRGDAGLFVSSEPSDAQVFLDGVALGRTSHL
                     DVAMVVPGRYQLEVRKEGFLPDVRMVELRSADTAFVHVVLTAISGGSIQVIANPRAHV
                     FLDGVEVGRSPLTVAALPGTHRLTLTREGF"
     gene            989..>1525
                     /locus_tag="PKN52_09445"
     CDS             989..>1525
                     /locus_tag="PKN52_09445"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNR00216.1"
                     /translation="MRYLVLSDVHANLPALEAVTTHAKKQGYDHVMFLGDAVGYYPHA
                     EEVVQRLIALEPTVRILGNHDSALLELVNGDTGDHWAAGVVMNVLERQLVTLSGDSLR
                     FLNALVPRHSQRGFEAAHGALAQQWDYLSTVTVAQTNVGLMTERVLFVGHTHVPKVFA
                     TVTKGPQELWRTVTFKEAS"
BASE COUNT          255 a          510 c          536 g          224 t
ORIGIN      
        1 tggaagccct cgcgcgtgag cgtgagccgg tgggtgccgg gcagggccgc gacggtaagc
       61 ggactcctgc ccacctccac gccgtcgagg aagacgtgcg cccgcgggtt ggcgatgacc
      121 tggatgctgc cgcccgagat ggccgtgagg acgacgtgca cgaacgccgt atcggccgaa
      181 cgcagctcga ccatgcggac gtccggtagg aagccctcct tgcgcacctc gagctggtag
      241 cgccccggca cgaccatggc gacgtcgagg tggctggtcc tgcccaaggc gaccccatcg
      301 aggaacacct gggcgtcgct aggctcgctc gaaacgaaca gcccggcgtc gcccctttgc
      361 aggtcgacga tggcgctcgt gaacgtcgcg accgccgcgg cgagttgggc tatcgccgtg
      421 cgctggtcgt cggggtcgag cggcaggctg atcaggtcgg tggcccggtc gcgttcagag
      481 tcgaccacga tcatattgac ggcgtagacg tcgtcgccgg aggcgctgga gcgtgccacc
      541 tcgccgctga cggcgtagcg ggtctgatcg agctcggcta tcagcatggc gaactcgggc
      601 tcgaggctgc cggcgatgcc cggggtgatc agttcgccgt tccgcacctc taggcccgtg
      661 gccgccccga cggccgagcg gaacgccggc atgaagctac gcaccaacgc gtcggggacg
      721 tcgccgcgcg tgccgaacgt cggcacggaa acctgcgcat gtgccaaggt gaacaagcac
      781 gcaagtagca cgacgcccca tctgagcagg ggggagagac gacgcggccg gcgcacacag
      841 caagtatagc cgcaagcgcg ctctttctct tgaggcgtcg gggcgcacgg taggccgact
      901 ggcgggcgtg acgcaccggg cgacccggcc ctcgggcgcg gccaccggat ggggggcggg
      961 cggaacccgc cctggtatcg ttgcccccat gcggtaccta gtgctgagcg acgtccacgc
     1021 caatctcccg gcgctggagg ccgttaccac acacgcgaag aagcaaggct acgaccatgt
     1081 gatgttcctc ggcgacgccg tcggctacta cccccacgcc gaagaggtcg tccagcgcct
     1141 gatcgccctc gaaccgaccg tccgcatcct cggcaaccac gactcggccc tgctggaact
     1201 ggtcaacggc gacacgggcg accattgggc cgcgggggtc gtgatgaacg tcctggagcg
     1261 ccaactcgtc acgttgagcg gcgactccct caggttcctg aacgccctgg tgccgcgcca
     1321 ctctcaacgc ggcttcgagg cggcccacgg cgcgctcgcc cagcagtggg actacctgtc
     1381 gacggttacg gtcgctcaga cgaacgtggg gctgatgacg gagagggtcc tgttcgtcgg
     1441 tcatacgcac gtgcctaagg tgttcgcgac cgtgacgaag ggtccgcagg aactttggcg
     1501 cacggtgacg ttcaaggagg cgagc
//
LOCUS       DAOQYE010000611         1523 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_103394_length_1523_cov_2.270436, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000611 DAOQYE010000000
VERSION     DAOQYE010000611.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103394_length_1523_cov_2.270436"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="PKN52_09450"
     CDS             complement(<1..476)
                     /locus_tag="PKN52_09450"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HNR00217.1"
                     /translation="MPSSPWRAPQAVEGRLRAPIAGGAPSGASESDAAVGLDARVPTG
                     PHAELERRLRDVVKGEVRFDALARALYSTDASPYRIAPIGVVVPLDEDDVKAAVRVAH
                     DLEVPILPRGGGTSLAGQTVAAALVIDMSKHLTALLELHVEERWVRVQPGLVRDQL"
     gene            complement(439..>1523)
                     /locus_tag="PKN52_09455"
     CDS             complement(439..>1523)
                     /locus_tag="PKN52_09455"
                     /inference="COORDINATES: protein motif:HMM:NF037628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00218.1"
                     /translation="STPGISAVAGLRAASEHLLRYGGHKQAAGFALDMENFGAFQDAI
                     YDYARRFPRPAPEVVADAIIGGDEIEGGLWRAVQELEPFGEGHPAPLFALSDTLDTAR
                     AVGQEGSTLQLRIGGVKGVAWRMGELAASLPVGALVNAAITLEESEFREKRTLEFRAA
                     AVRAAAPLVLAPDDEREPDSTGAPRVRRSAAAQATDPYAVTDLGPDPVIALERLAGSD
                     AEVSLALTPDAIHALERMAKEYPTVSELRRGLSALMRGAPLPFLEPKVERIRTALAEL
                     GLIDDQARAVRLPPGTRVSPFESPSLMEGLLRRYRLRTFVHAYRHLDDAGLAMTLKRL
                     FGPEAGRAPDVYLPDAKQPVESATSR"
BASE COUNT          205 a          530 c          555 g          233 t
ORIGIN      
        1 ttgagttggt cgcggacgag accgggctgc acgcgtaccc agcgctcctc gacgtgcagc
       61 tcgagcagcg ccgtgaggtg tttcgacatg tcgatgacga gagccgccgc cacggtctga
      121 cctgcgagac tagtgccgcc gccgcgcggg aggatgggga cttcgaggtc gtgagcgacg
      181 cggaccgcgg ctttgacgtc gtcctcgtcg agcggcacca ccaccccgat cggggcgatg
      241 cggtacgggc tggcgtcggt ggagtagagc gcccgcgcga gggcgtcgaa gcgtacctcg
      301 cccttgacca cgtccctcag gcgccgctcc agttcggcgt gagggccggt cgggacgcgc
      361 gcgtctagcc cgacggctgc gtcggactcg ctggccccgc tcggcgcccc gcctgcgatc
      421 ggcgcccgca ggcgcccttc aacggcttgt ggcgctctcc acgggctgct tggcatcggg
      481 caagtatacg tctggcgcgc gcccggcctc ggggccgaac aggcgcttga gggtcatggc
      541 gaggccggcg tcgtcgagat gccggtaggc gtgcacgaag gtgcggagac ggtagcgtcg
      601 gagcagcccc tccatgaggc tcggggactc gaacggcgac acgcgcgtgc ccggcggtag
      661 ccgcacggct cgcgcctggt cgtcgatgag ccccagttcc gccagcgccg tcctgatgcg
      721 ctctaccttc ggctcgagga aaggcagcgg agcgccccgc atgagcgcgc tcagcccccg
      781 cctgagctcg gagacggtgg ggtactcctt ggccatgcgc tcgagcgcgt gtatggcatc
      841 gggggttagg gcgagcgaga cttcggcgtc gctgccggcg aggcgctcca gagcgatgac
      901 ggggtccggc ccgaggtcgg tcacggcgta ggggtcggtc gcttgcgctg cagctgatcg
      961 ccttacgcgg ggcgcaccgg tgctatccgg ctctcgctcg tcgtctgggg ccaagactaa
     1021 cggcgcggcc gcccgcaccg ccgccgcgcg gaactcgagc gtgcgcttct cgcggaactc
     1081 gctctcctcc agggttatcg cagcgttgac gagcgccccg acgggcaggc tggcggccag
     1141 ttcgcccatg cgccaggcca cgcccttcac gccgccgatc ctgagctgca gcgtgctgcc
     1201 ctcctgaccg accgcgcgtg ccgtgtccag agtgtcagac agtgcgaaga gcggcgccgg
     1261 gtgaccctct ccgaacggct ccagttcctg gacggcgcgc cacaagccac cttcgatctc
     1321 gtcgccgccg atgatcgcgt cggccactac ctcgggtgcc ggcctgggga agcgtcgcgc
     1381 gtagtcgtag atggcgtcct ggaaggcccc gaagttctcc atgtcgagcg cgaagccggc
     1441 agcctgcttg tgaccgccgt acctgaggag gtgctccgac gccgcgcgca ggcccgccac
     1501 ggccgagatg ccaggggtcg agc
//
LOCUS       DAOQYE010000612         1522 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_103477_length_1522_cov_2.965235, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000612 DAOQYE010000000
VERSION     DAOQYE010000612.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103477_length_1522_cov_2.965235"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /locus_tag="PKN52_09460"
     CDS             complement(<1..573)
                     /locus_tag="PKN52_09460"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNR00219.1"
                     /translation="MRSPTRGARTALRLVSYASILALWIVGARLLGSNVLPGPGETFA
                     FILREADRGVLLPHLWITAQRVLAAFVVGMLLGTVLGAAMGLSRRADDLLQGWLVVTL
                     TVPRIVLFVVAYLSIGLSDRALILALVITIIPTIVASVREGARALDGGLIEMAVAFRR
                     SRSSIWRRIVFPQLTPYLVGTARGALALTWK"
     gene            complement(586..>1522)
                     /locus_tag="PKN52_09465"
     CDS             complement(586..>1522)
                     /locus_tag="PKN52_09465"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNR00220.1"
                     /translation="AQGGTSSPPVVNAAMEATGTFSWVLHGMEHFGTAEANGIRVVAT
                     PYASKQATEIALRSGEADVKVDDFLGPVLLRDAGIMASGIYPYATSVGGLVVPAGSEI
                     TSIADLAGKTIAAGSLRDKSLLILRALAVSQYGFDPQVDAEVVAAAPPLMAELTARGE
                     VDAALPLWHWVPRMEATGAREVMSVADMLGGLGLPTDLPNLVVVARDDMPAELKTAFL
                     ATLRDTFDIMLATPSDDPFWQEILDLELYSLPDRSQFPSVVERWKMGTTTTWTQESID
                     GLTAMVERLVELAGPEVVGVDGVDPAAYTLEFLPD"
BASE COUNT          270 a          511 c          520 g          221 t
ORIGIN      
        1 cttccacgtg agcgccagcg cgccacgcgc cgtgcccacc aggtaaggcg tcagttgcgg
       61 gaagacgatc cggcgccaga tgctagagcg ggagcggcgg aaggccaccg ccatctcgat
      121 gaggccgccg tccagcgcgc gtgcgccctc gcgcactgac gcgacgatgg tcggaatgat
      181 cgtgatgacg agggctagga tcagcgcgcg gtcggacagg ccgatgctca agtaggccac
      241 gacgaacagg acgatgcgcg ggactgtgag ggtgacgacg agccagccct gcaacaggtc
      301 gtcggcccgg cgcgacaggc ccatggcggc accgagcacc gtgccgagaa gcatgcctac
      361 gacgaacgcc gcgagtaccc tctgggccgt gatccagagg tgggggagca gcacgcccct
      421 gtccgcctcg cgcaggatga aggcgaaggt ctcgccgggg ccgggcagga cgttcgaccc
      481 gaggagccgt gcgccgacga tccacaaggc gaggatggag gcgtacgaga cgaggcggag
      541 ggcggtccgc gcgccccggg taggcgaacg caccgctagt ccgactcagt ccggcaggaa
      601 ctcgagggta taagcggcag gatcgacccc gtccacgccc actacctcag gaccggcgag
      661 ttcgactagg cgctcgacca tggccgtcag accgtcgatc gactcttgcg tccacgtggt
      721 cgttgtaccc atcttccaac gctccaccac gctcgggaac tgactccgat cgggcagcga
      781 gtagagttcc aggtctagga tctcttgcca gaaggggtcg tcggagggcg tcgccaacat
      841 gatgtcgaag gtgtcgcgca gggtcgcgag gaaggcggtc ttgagctcgg cgggcatgtc
      901 gtcgcgcgcc acgaccacca ggttggggag atccgttggt agcccgaggc cacccaacat
      961 gtcggcgacg ctcatgacct cacgtgcgcc cgtcgcctcc atccttggta cccaatgcca
     1021 tagcggcagc gcggcgtcca cctcgcccct cgcggtcagc tccgccatga gcggtggcgc
     1081 ggccgccacg acctccgcgt cgacttgcgg gtcgaaaccg tactgagaca cggccagcgc
     1141 cctcaggatg agcaagctct tgtcgcgcag actgccggcg gcgatcgtct tgcccgcgag
     1201 gtcggcgatg ctcgttatct cggaaccggc cggcaccacg agccccccta ctgaggtcgc
     1261 gtacgggtag atgccgctgg ccatgatgcc cgcgtcgcgc agtagtaccg gcccgaggaa
     1321 gtcgtccacc ttgacgtcag cctcccccga gcgcagggct atctcggtcg cctgcttcga
     1381 ggcgtaaggc gtggcaacga cgcggatacc gttggcctcg gccgttccga agtgctccat
     1441 gccgtggagg acccaggaga acgtccccgt tgcctccatg gccgcgttaa ccaccggtgg
     1501 agaagacgta cccccctggg cg
//
LOCUS       DAOQYE010000613         1519 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_103806_length_1519_cov_3.303279, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000613 DAOQYE010000000
VERSION     DAOQYE010000613.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103806_length_1519_cov_3.303279"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="PKN52_09470"
     CDS             complement(<1..464)
                     /locus_tag="PKN52_09470"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HNR00221.1"
                     /translation="MLKGSLKSAPLSDVFQLIALSQKSGILTVERKDRHARIYFTLGK
                     VAYAHMRSGAHLGEVLVRMDLVTALEVQEILMTQRRENPGTLLGRMAVERGILGEEDL
                     ARAIERQAFDVVGEVLTWSDGAFEFHDADPNASQVPFGSGIDALVLLMRVIQ"
     gene            complement(517..1518)
                     /gene="ruvB"
                     /locus_tag="PKN52_09475"
     CDS             complement(517..1518)
                     /gene="ruvB"
                     /locus_tag="PKN52_09475"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HNR00222.1"
                     /translation="MDEVRMRPASLADYVGQRRLKRKLEVYLGAARDRGEALDHVLLY
                     GPPGLGKTTLAHIIAAELGVGIRVTSGPAIEKPGDLAAILTNSLEPGDVLFIDEIHRL
                     GRVAEEHLYPAMEEFKIDIVLGQGPAARSIRLELPRFTLIGATTRPGLISGPLRSRFG
                     IIEHLDYYSDADLAQSVKADARQLGYALNDDAALEIGRRSRGTMRIAKRLLRRVRDYA
                     QVAGEDAIDVRRAKLALDALDIDDAGLDRRDRAILGALVERFAGGPVGLDTLATAVAE
                     DRATLEEVYEPFLIQRGLLVRTPRGRMATPATYGHLGLPVPQAQAAPLFTGDQEGRA"
BASE COUNT          214 a          520 c          554 g          231 t
ORIGIN      
        1 ctctggatga cgcgcatgag caggacgagc gcatcgatgc cgctgccgaa cggcacctgg
       61 gacgcgttcg ggtccgcgtc gtggaactcg aaggcgccgt ccgaccacgt gagcacctcg
      121 cccacgacgt cgaacgcctg acgctctatg gcgcgcgcca ggtcctcctc gccgaggatg
      181 ccccgttcga cggccatgcg accgagcagc gtgcccgggt tctcgcgccg ctgcgtcatc
      241 aggatctcct gcacctctag ggcggtgacg aggtccatgc gcacgagcac ctcgcccaag
      301 tgggcgccgg agcgcatatg ggcgtatgcg accttgccga gggtgaagta gatgcgcgcg
      361 tgcctgtcct tgcgctccac cgtgaggatg ccggacttct ggctcagggc gatgagctgg
      421 aacacgtctg acaggggcgc gctcttgaga ctgcctttca gcatggcgcc cccgaggccg
      481 gcggccggcg gccaggaccc gccttcgccg ccgtgctcac gcccgcccct cttggtcgcc
      541 ggtgaacagc ggcgccgcct gggcttgcgg caccggcagc cccaggtggc cgtaagtggc
      601 cggggtggcc atgcgaccgc gcggcgtgcg cacgagcagc ccgcgttgga tgaggaacgg
      661 ctcgtagacc tcctcgagcg tggcccggtc ctccgccacg gcagtagcga gcgtgtcgag
      721 gccgacgggt ccgccggcga accgctccac cagggcgccg aggatggccc tgtcccgccg
      781 gtcaaggccg gcgtcgtcga tgtcgagggc gtcgagggca agcttggccc gcctcacgtc
      841 gatggcgtcc tccccggcca cctgggcgta gtcgcggacg cgcctgagca gacgcttggc
      901 gatacgcatc gttccccgcg agcgcctgcc gatctcgagc gcggcgtcgt cgttgagcgc
      961 gtaacccagc tgccgggcgt ccgccttgac ggactgcgcc aggtcggcgt cggagtagta
     1021 gtcgaggtgc tcgatgatgc cgaagcggct gcgcaaggga ccggagatga gcccggggcg
     1081 tgtcgtcgcc ccgatcaggg tgaagcgcgg cagctccagg cggatggacc gcgccgccgg
     1141 cccctggccg agcacgatgt cgatcttgaa ctcctccatc gcagggtaga ggtgctcctc
     1201 cgcgacgcgg ccgaggcggt ggatctcgtc gatgaagagg acgtcgcccg gctcgaggga
     1261 gttggtgagg atggccgcca ggtcgccggg cttctcgatg gccggtcctg acgtgacccg
     1321 gatgccgact ccgagctcgg cggcgatgat gtgggcaagc gtcgtcttgc cgaggccggg
     1381 tgggccgtac agcaggacgt ggtcgagggc ctcgccgcgg tcgcgggctg cgccgaggta
     1441 cacctcgagc ttgcgtttca gccgccgctg accgacgtag tcggccaaag acgccggccg
     1501 catgcgcacc tcgtccacg
//
LOCUS       DAOQYE010000614         1518 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_103953_length_1518_cov_2.414901, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000614 DAOQYE010000000
VERSION     DAOQYE010000614.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103953_length_1518_cov_2.414901"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..674
                     /locus_tag="PKN52_09480"
     CDS             <1..674
                     /locus_tag="PKN52_09480"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HNR00223.1"
                     /translation="HAALTADAAPLPEAETVFDRVLAGTATDAERRAFAANMLMVMAR
                     MSAEDGLVMQLHVGSLRDHHRPTFERFGRDKGADMPIAGGWTRQLAPLLNELGAHERF
                     RLVLYTLDETTYSRELAPLAGFYPALRLGAPWWFFDSVLGMERYLDAVAETAGIYNLA
                     GFVDDTRAYPSIPARHDVWRRVTCDWLAGRVVRGLIDEDQAPDLAHALAYDLTKETYR
                     LGAAG"
     gene            691..>1518
                     /locus_tag="PKN52_09485"
     CDS             691..>1518
                     /locus_tag="PKN52_09485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00224.1"
                     /translation="MTPYLHFSVDDVFGCLHDAFTGAYASPFAQPTFAYLRDLHERYG
                     LCVSLYCFAEDAAVTGWRLAHADERFAAEFAAAASWLRFGFHALNAGTRYSAGATSAE
                     AARAHHDEVTAATARFATPAALDRAPRLHYYTGSLAAVRAMADGPHGLLGLLTADDDR
                     AEVYYLDDETRRLVREEGSLRDPTTGLELFSTDLRLERLEQDVGEIVAVAGRRAAVGE
                     GPLSDTPEVRPGPNTHAGSDAHAKPGVRPGPGVHAKPDAQAGPGGIGSFLKIEGYATS
                     "
BASE COUNT          207 a          584 c          525 g          202 t
ORIGIN      
        1 accatgcggc cctcacggcc gacgcggcgc ccctccccga ggcggaaacc gtcttcgacc
       61 gcgtgctggc cggcacggcc accgacgccg agcgccgcgc cttcgccgcc aacatgctca
      121 tggtcatggc ccgcatgagc gccgaggacg gcctcgtcat gcagcttcac gtcggctccc
      181 tgcgcgacca ccaccgcccg accttcgagc gcttcgggcg cgacaagggc gccgacatgc
      241 cgatcgccgg cggctggacg cgccagctcg cgcccctcct gaacgagctc ggcgcgcacg
      301 agcgcttccg gctggtcctg tacaccctcg acgagacgac gtactcgcgc gagctggcgc
      361 cgctggccgg cttctacccg gccctgcgcc tcggtgcgcc gtggtggttc ttcgactcgg
      421 tcctcggcat ggagcgctac ctggacgccg tggccgagac ggccggcatc tacaacctcg
      481 cgggtttcgt cgacgacacg cgcgcctacc cctccatccc cgcgcgccac gacgtgtggc
      541 ggcgcgtgac gtgcgactgg ctggccggcc gcgtcgtgcg gggcctcatc gacgaggacc
      601 aggcccccga cctcgcccac gcgctcgcgt acgacctcac gaaggagacc taccgcctgg
      661 gcgccgccgg ctaacgcgac accggccgac gtgacgccgt acctccactt cagcgtcgac
      721 gacgtcttcg gctgcctgca tgacgccttc acgggcgcgt acgccagccc gttcgcgcag
      781 cccacgttcg cctacctgcg cgacctgcac gagcggtacg ggttgtgcgt gagcctttac
      841 tgcttcgcgg aggacgcggc ggtgaccggc tggcgcctgg cgcacgccga cgagcgcttc
      901 gcggccgagt tcgccgctgc cgcatcgtgg ctgcggttcg gcttccacgc cctgaacgcc
      961 ggcacgcgct actcggccgg ggcgacgtcg gcggaggccg cccgcgctca ccacgacgag
     1021 gtgacggccg ccaccgcgcg cttcgcgacg cccgccgcgc tcgaccgggc gccgcgcctg
     1081 cactactaca cggggtcgct ggcagccgtg cgggccatgg ccgacggacc ccacggcctc
     1141 ctcggcctcc tgacggcaga cgacgaccgc gcagaggtct actacctcga tgacgagacg
     1201 cggcgcctgg tgcgggagga gggcagcctg cgcgacccga cgaccggact ggagctcttc
     1261 tcgacggacc tgcgcctcga gcggctcgag caggacgtgg gtgagatcgt cgcggtggcg
     1321 gggcggcgcg cggcggtcgg ggagggaccg ctctccgata cgccggaagt gcgccccggg
     1381 ccgaacacgc acgccggatc ggacgcgcac gccaagccgg gtgtgcgccc cgggccgggt
     1441 gtgcacgcca agccagatgc gcaagccggg cctgggggca tagggtcgtt cctgaaaatc
     1501 gagggatatg caacatcc
//
LOCUS       DAOQYE010000615         1515 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_104338_length_1515_cov_2.213014, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000615 DAOQYE010000000
VERSION     DAOQYE010000615.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104338_length_1515_cov_2.213014"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(119..1462)
                     /locus_tag="PKN52_09490"
     CDS             complement(119..1462)
                     /locus_tag="PKN52_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HNR00225.1"
                     /translation="MVAGSAAAKAGVAPGWELLSVNGQPIPDVLAYRRELQAGAAELL
                     VRAPEGAEVAFGVSWEEPGLEFDDVIFDGIRLCANHCDFCYIHQMPKGMRKSLYIMDD
                     DFRTSFLYGSFVTLTNLREGDLRRIVDEHLSPLYVSVHTANEAKRADLMKWWPNKVAT
                     EEALSVRNMLERLESIDIYTQMVLVPGRNDGDDLDETLAYLASRPNVLAAAAVPVGLT
                     GHRSHLAALEPYTPEQASDVVARVHRFQQRMLAERGTRFVFASDEFYIRAGLPLPEDE
                     AYEGYTMLENGVGMVRDFLSSGIPNLPDGLDCPRRVLLATGRLFAPVLRDALEPLAGV
                     DGLELEVRAITNRTFGEVTTVAGLLAGRDILGGVERGEADLLLLSPNMFKYGTETMLD
                     DLTRSDLQAELGMRIAVGGTNLAELVDTVLTGEVHDHLPSFGFSTHAIKEAAKQH"
BASE COUNT          242 a          497 c          530 g          246 t
ORIGIN      
        1 cagcatgccg ccgtaggcga acggcagggc gaacagggtg tgctcgagct tgacgaacga
       61 cgcgagcgtc gcgaacgtca acctcccccc gcctcccgtc cgcacgccgc ccgtcacgtc
      121 agtgctgttt ggcagcttcc ttgatggcat gggtcgagaa gccaaagctc ggcaggtggt
      181 cgtgcacctc gccggtgagg accgtgtcga cgagctcggc caggttcgtg ccgccaacgg
      241 cgatccgcat gccgagctcg gcctggaggt cgctgcgggt caggtcgtcg agcatcgtct
      301 cggtgccgta cttgaacatg ttgggggaca ggagcaggag gtcggcctcc ccgcgctcga
      361 cgccgcccag gatgtcccgt ccggccagca ggccggccac cgtggtgacc tcgccaaacg
      421 tgcggttcgt gatcgcgcgc acttcgagct cgagcccgtc cactccggcg agcggctcga
      481 gggcgtctct cagcacgggg gcgaagaggc ggcccgtcgc cagaagtacg cgcctcggac
      541 agtcgagtcc gtcgggtagg ttggggatgc cgctcgacag gaagtcgcgg accatgccga
      601 cgccgttctc gagcatggtg tagccctcgt acgcctcgtc ctcgggcaac ggaaggccgg
      661 cgcggatata gaactcgtcc gaggcgaaca cgaagcgggt cccgcgctcc gccagcatcc
      721 gctgctggaa ccggtgcacg cgcgcgacga cgtcggaggc ctgctcaggc gtgtacggct
      781 ccagagcggc gaggtgggaa cggtggccag tgaggccgac cggcacggcc gccgcggcca
      841 gtacgttcgg gcgcgaggcg aggtaagcga gcgtctcgtc cagatcgtca ccgtcgttgc
      901 ggccggggac cagaaccatc tgagtgtaga tgtcgatgct ttcgaggcgc tcgagcatgt
      961 tgcggacgct gagggcctcc tccgtggcga ccttgttcgg ccaccacttc atgaggtcgg
     1021 cgcgcttggc ctcgttggcg gtatgaacgc tcacgtagag cgggctcaag tgctcgtcga
     1081 cgatgcgccg caggtcgccc tcgcgcaagt tcgtcagggt gacgaaggag ccgtagagga
     1141 acgacgtgcg aaagtcgtcg tccatgatgt agaggctctt acgcatgccc ttcggcatct
     1201 ggtggatgta gcagaagtcg cagtggttgg cgcagagtcg gataccgtcg aagatgacgt
     1261 cgtcgaactc caaccctggc tcctcccacg agacgccgaa cgcgacctcg gcgccctccg
     1321 gcgccctcac gagcagctcg gccgctcctg cttggagttc acggcgatag gcgagcacgt
     1381 cggggatggg ctggccgttg acgctcagga gctcccaccc gggcgccact cccgccttgg
     1441 ccgccgcgga acccgccacc accgcgcgaa tgcgggccgg cgtgaccttg accggctgcg
     1501 gttctttgcg gctca
//
LOCUS       DAOQYE010000616         1513 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_104551_length_1513_cov_2.384088, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000616 DAOQYE010000000
VERSION     DAOQYE010000616.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104551_length_1513_cov_2.384088"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="PKN52_09495"
     CDS             complement(<1..586)
                     /locus_tag="PKN52_09495"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HNR00226.1"
                     /translation="MVVLPLGSTEQHGPHLPLATDALLADRLLDAALALLPEQVRVWR
                     LPTLPYGKSNEHTGFAGTLTLSHQTLAGVLHDVARGVAEAGFTRLALFSGHGGNTPTL
                     DVVARDVRANTGLLCFVIDPGIQFSAVTGLSERERDLGYHAGQVETSLMLALAPELVR
                     MERAVAEFPPASPSRIGLAGPARKAWLTSDVRKSS"
     gene            complement(710..>1513)
                     /locus_tag="PKN52_09500"
     CDS             complement(710..>1513)
                     /locus_tag="PKN52_09500"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNR00227.1"
                     /translation="RYGATMNLQICAFPQEGLRTNEESFELMERALVSGADVVGGLPW
                     VEWTDAAASEHVDFCFDLAMRFDKDIHFLCDDAPDPMSRTLEMVAAKTIATGFKGRVS
                     SSHNGALRHYPDAHAVRVINMLKQAGVNMVANANVNSLGTYTRVPELLAAGVNVSFGQ
                     DDLDNFYYPLGRADMLDVMNFTVHMAHLATPRGFETAFDIGTFNGARTLRLDRYGIAV
                     GNPANMLVFAGRTAHEVIQMQSDRNYVISKGQVVCETNRTSTLRLIGAQ"
BASE COUNT          242 a          483 c          536 g          252 t
ORIGIN      
        1 cgctgctctt gcggacgtcc gacgtcagcc aggccttccg cgccggcccc gcgaggccga
       61 ttcgactcgg cgaggccgga gggaactcgg ccacggcgcg ctccatacgg acgagttctg
      121 gcgccagcgc gagcatgagc gaagtctcca cctgaccggc gtgatagccg aggtcacgct
      181 cgcgctccga gagcccggtg acggcgctga actggatgcc ggggtcgatc acgaagcaga
      241 ggagtcccgt gttggcgcgc acgtcgcgcg ccacgacgtc cagcgtcggc gtgttgccgc
      301 catggccgct gaagagcgcg aggcgcgtga agccggcctc cgcgaccccg cgcgccacgt
      361 cgtgcaggac gccggctagc gtctggtgcg atagcgtcaa cgtcccggcg aagccggtgt
      421 gctcgttgct cttcccataa gggagcgtcg ggaggcgcca gacgcggact tgctcgggca
      481 ggagcgccaa agccgcgtcc aataggcggt ccgcgagcag ggcgtcggtc gccagcggta
      541 gatgcgggcc gtgctgctcg gtcgaaccga ggggcaggac gaccaagccg ccctccttgt
      601 ccagggacgc gacctcaacg tatgtgagtt cggccagatc ccgcctaggc ggcatgcggg
      661 ccggcgacca gcaccggttt gggcctcgcg tggtcatgaa ccgcctgcat cactgcgcac
      721 cgatgagccg cagggtgctg gtgcggttcg tttcgcacac gacctggccc ttcgagatca
      781 cgtagttcct gtcggactgc atctgtatga cctcgtgggc agtgcggccc gcgaagacga
      841 gcatattcgc ggggttgccg accgcgatgc cgtagcggtc gagccgcagg gtgcgggcgc
      901 cgttgaacgt accgatgtcg aaggcggtct cgaaaccgcg cggcgtcgcg agatgcgcca
      961 tgtggacggt gaagttcatg acgtcgagca tgtcggcgcg ccccaggggg tagtagaagt
     1021 tgtccaggtc gtcctggccg aagctcacgt tcacacccgc cgccaagagc tccgggacgc
     1081 gcgtgtaagt gccgagcgag ttgacgttcg cgttggcgac catgttgacg ccggcctgct
     1141 tgagcatgtt tatgacccgc accgcgtgcg cgtcggggta atgcctgagc gcaccgttgt
     1201 ggctcgagga cacgcggcct ttgaagccgg tggcgatggt cttggccgcc accatctcca
     1261 aagtgcgcga catggggtcg ggagcgtcgt cgcagaggaa gtggatgtcc ttgtcgaagc
     1321 gcatggcaag gtcgaagcag aagtcgacgt gctcgctcgc cgcagcgtcc gtccactcga
     1381 cccatggaag cccgcccacc acgtccgcgc cggataccag ggcgcgttcc atgagctcga
     1441 agctctcctc gttcgtcctg aggccttctt gcgggaatgc gcagatctgg aggttcatgg
     1501 tggcgccgta gcg
//
LOCUS       DAOQYE010000617         1513 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_104552_length_1513_cov_2.383402, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000617 DAOQYE010000000
VERSION     DAOQYE010000617.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104552_length_1513_cov_2.383402"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            118..>1513
                     /locus_tag="PKN52_09505"
     CDS             118..>1513
                     /locus_tag="PKN52_09505"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNR00228.1"
                     /translation="MTYTQPARRNGRRSIALGCALAVLLTVGAAFANAELIRIQRDAG
                     QWVMANGNYQGWNYSTLNQITNYNVKNLQVKWTLQLGVTDSLEAPPIVVGNTMYILTP
                     KPNTVYALDLTREGFIKWSYRVDQPNLAQAKACCGAQSRGISYADGKIVLNSLDGQLM
                     ALDANTGAVLWNTQVTDLSITETTTNAPLIVGNNILIGNEGGERGVRGWVAAYDLQTG
                     DQVWKYYNTGPNADMGIGDRWNPFYEVDKRWTEPGVDTWYGDSWKLGGGTAWGYFTYD
                     PESNLFYYGTANCGPWNPDYRRDPATAPGLDQYLSKYCASLIARDATTGDMVWAYSTT
                     PQDQWDFDEPGQNFVADLTIDGQVVKALIKPARNGFFYVFDRLTGELVRDVFAYTNVN
                     WATGVDMETGVPIYNEDALTFTDIPTPISVCPFIAGNNWYNDAYSPSTGLVYFQAENR
                     CATFTGTEAEYTPGE"
BASE COUNT          303 a          544 c          449 g          217 t
ORIGIN      
        1 cgcagaggag gtgagcactc ggcggtaagg gcctgacgca ccaggcccaa gcagcgaagt
       61 gtcgcagatc tcgcccgacc ttccacgtac tctagaaagg tattggtggc aaccagaatg
      121 acgtatacgc aacccgccag acgcaacggc aggcgctcca tcgcgctcgg ctgcgcgctc
      181 gccgtcctcc tcacggtcgg tgcggccttc gccaacgccg agctcatccg gatccagcgc
      241 gatgccggcc agtgggtgat ggcgaacggc aactaccaag gctggaacta cagcacgctc
      301 aaccagatca cgaactacaa cgtcaagaac ctgcaggtga agtggacgct gcagctcggc
      361 gttacggact ccctcgaggc gccgcccatc gtcgtcggca acaccatgta catcctgacg
      421 ccgaagccga acacggtgta cgcgctcgac ctgacgcgcg aaggcttcat caagtggtcc
      481 taccgcgtcg accagccgaa cctcgctcag gccaaggcgt gttgcggcgc ccagtcgcgc
      541 ggcatcagct acgccgacgg caagatcgtc ctcaactcgc tcgacggcca gctcatggcg
      601 ctcgatgcga acacgggcgc cgtgctctgg aacacgcagg tcacggacct gagcatcacc
      661 gagaccacga ccaacgcccc cctcatcgtc ggcaacaaca tcctgatcgg caacgaaggc
      721 ggcgagcgcg gcgtgcgcgg ctgggtggcg gcctacgacc tccagacggg cgaccaggtc
      781 tggaagtact acaacacggg ccccaacgcc gacatgggca tcggcgaccg ctggaacccc
      841 ttctacgaag tggacaagcg ctggaccgag ccgggcgtcg acacgtggta cggcgactcc
      901 tggaagctcg gcggcggcac ggcttggggc tacttcacct acgaccccga gtcgaacctc
      961 ttctactacg gcaccgccaa ctgcggccct tggaacccgg actaccgccg cgacccggcc
     1021 accgcgccgg gcctcgacca gtacctctcc aagtactgcg cctcgctcat cgcgcgtgac
     1081 gcgacgacgg gcgacatggt ctgggcgtac tccaccacgc cgcaggacca gtgggacttc
     1141 gacgagccgg gccagaactt cgtggccgac ctcaccatcg acggccaggt cgtcaaggcc
     1201 ctcatcaagc ccgctcgcaa cggcttcttc tacgtgttcg atcgcctgac gggcgagctg
     1261 gtgcgcgacg tcttcgcgta cacgaacgtc aactgggcga cgggcgtcga catggagacc
     1321 ggcgtcccca tctacaacga ggacgcgctc accttcaccg acatccccac cccgatctcg
     1381 gtctgcccgt tcatcgcggg gaacaactgg tacaacgacg cctactcgcc cagcacgggc
     1441 ctcgtctact tccaggccga gaaccgctgc gccaccttca cggggacaga ggctgagtac
     1501 acgcccggcg aga
//
LOCUS       DAOQYE010000618         1512 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_104606_length_1512_cov_4.317090, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000618 DAOQYE010000000
VERSION     DAOQYE010000618.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104606_length_1512_cov_4.317090"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1013
                     /locus_tag="PKN52_09510"
     CDS             <1..1013
                     /locus_tag="PKN52_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNR00229.1"
                     /translation="TITAWNFPVYNIVRTWAAALAAGNTVVGRPSEFTPRSAMLLAQA
                     LQEGGAPAGVVNVVNGDPEAMGQAFLDDARVRKIAFTGSPRVGRLLMDGASRTLTRLA
                     LELGGNAPVIVFPDVPDLGRVAALAARFKARNAGQVCIAPQRYFVHASVAEEFTARVV
                     DAMRDLKVGHGLEDGVQVGPLINARQLGRVEELVASATADGARVALGGARLQRSGYFY
                     APTVVTDVTPGSRLYEEEIFGPVLPITTFTTASEVLALANATEYGLAAYLFTADLNTA
                     IAMAERLEFGMVGVNDWMPVSPEAPFGGVKGSGIGRETGSEGIQEYMEQKAVFIGGVE
                     LP"
BASE COUNT          206 a          540 c          536 g          230 t
ORIGIN      
        1 ccaccatcac ggcctggaac ttccccgtct acaacatcgt gaggacctgg gcggccgcgc
       61 tcgccgccgg caacacggtg gtcggccggc cgtcggagtt cacgccgcgc tcggccatgc
      121 tcctcgccca ggctttgcag gagggcgggg cgccggccgg cgtcgtgaac gtcgtcaacg
      181 gcgaccccga ggccatgggt caggccttcc tcgacgacgc gcgcgtgcgc aagatcgcct
      241 tcaccggcag cccgcgcgtc ggcaggctcc tcatggacgg cgcctcgcgc accctgacgc
      301 gcctcgccct cgagctcggc ggcaacgctc ccgtcatcgt cttccccgac gtgccggacc
      361 tgggccgcgt cgcggcgctt gccgcgcgct tcaaggcgcg caacgccggg caggtctgca
      421 tcgcgccgca gcgctacttc gtgcacgcga gcgtggccga ggagttcacg gcgcgcgtcg
      481 tcgacgccat gcgcgacctc aaggtcgggc acgggctcga ggacggcgtg caggtcgggc
      541 cgctcatcaa cgccaggcag ttgggcaggg tcgaggagct cgtcgcttcc gccaccgccg
      601 acggggcccg ggtggcgctt ggcggggcga ggctccagcg ctccggctac ttctacgcgc
      661 cgacggtcgt cacggacgtg acgcccggca gccggctgta cgaggaggag atcttcggtc
      721 cggtgctgcc catcacgacg ttcaccaccg cttccgaggt gctcgcgctc gccaacgcca
      781 cggagtacgg gctggccgcc tacctcttca ccgccgacct caacacggcc atcgccatgg
      841 ccgagcggct ggagttcggc atggtcggcg tgaacgactg gatgcccgtc agcccggagg
      901 cgcccttcgg cggggtcaag gggagcggca tcgggcgcga gacgggctcg gagggcatcc
      961 aggagtacat ggagcagaag gcggtgttca taggaggcgt cgaactgccc tagggtcagg
     1021 cgtccgcgtc tacgtggcgt ggttcggtcc gacccggttc cggtttgccg gagactccct
     1081 tccagagtga gccaccgcga gcgtagagtg tgacgagcag taggccaccg tgtctccgcc
     1141 tgatccgacc tcatcatcac agcccgaccc catcgcgcag tccgtttccg cgggcgagga
     1201 cgttctcgtg cctggcgccg tcggtggggc tcgtgagggt gcgttggtcg gcagcgcggc
     1261 cgtggccgtt cgaggcacgg gcgagaccgc gtcaggctcg gaggctgaag gtggggtcgc
     1321 atccgctcca agcgcggccc ccctcgttcg tcgcacgatg cgcctctcag tcgtcgaggg
     1381 cagcctcacg caggtcctgc tcaactggac gacgggcagc gtcctcgtcg ggtacctgct
     1441 gcacctcggc gccacgccga cgcagatagc cctggcgggc agcatgccgc tcctcgcgca
     1501 ggtggcgagc cc
//
LOCUS       DAOQYE010000619         1511 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_104768_length_1511_cov_2.480769, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000619 DAOQYE010000000
VERSION     DAOQYE010000619.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104768_length_1511_cov_2.480769"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1492)
                     /locus_tag="PKN52_09515"
     CDS             complement(<1..1492)
                     /locus_tag="PKN52_09515"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNR00230.1"
                     /translation="MVHASDKLVELTGYSFEEFQGRSLGFLMRNDTDQEGGRMYDATT
                     ADEPSTSVVRIYRADGSLLWVEQRHYPVHAVDGDAATHVLTLFRDVTAEVHAGGAQAM
                     QLELSGSLEGDGRFFSYALLLHDDGRNEVVWVSDAWHQLTGYAVEHLKTQGFRRFIHA
                     EDRAQLEERLQGLRDQERRSDQYRLVTYGGKVMWVEDFASRRWRSDEAGITAVYGMVK
                     DITNARRETANLWRLAHIDSLTGLPNAHLLEDRVQQAQLHARRNGTGIALAILDLDNF
                     RFVNQTFSQRHGDRLIMEVSRRLRRTLRRTDTLARLGGDSFALLLGDLPTQRSVLPAL
                     EKVLAAVREPYADGSLTLQLAASVGVDVLHDGARAANHMLERATAALVRAKETNRGGF
                     RFYDDEIDLAMRTRIATERELRRAIAEDQLVLHYQPRVQLDDGSINSVEALVRWVHPV
                     RGLLKPMDFVPLVEESQLGPRLFEWVVERACRQAKRWQSQRTPRRVA"
BASE COUNT          220 a          513 c          527 g          251 t
ORIGIN      
        1 cggccacgcg ccttggcgtg cgctggctct gccaccgctt ggcctggcgg caggcccgct
       61 cgaccaccca ctcgaacagc cgggggccga gctgcgactc ctcgacgagc ggcacgaagt
      121 ccatcggctt gagtaggccc cgaacggggt gcacccagcg cacgagcgcc tcgacggagt
      181 tgatgctccc gtcgtcgagc tgcacgcgcg gttggtagtg cagcacgagc tggtcctccg
      241 cgatggcgcg ccggagctcc cgctccgtcg cgatacgcgt acgcatggcc aggtcgatct
      301 cgtcgtcgta gaagcggaag ccgccgcggt tcgtctcctt ggcgcgtacg agcgccgccg
      361 tcgcccgctc cagcatgtga ttggcggcgc gagcaccgtc gtgcagaacg tcgaccccga
      421 cgctcgcagc cagctgcaac gtgaggctcc cgtcggcgta cggctcgcgc acggcggcca
      481 gcaccttctc gagcgcgggt agcacggagc gctgtgtcgg caggtcgccg agcagcagag
      541 cgaacgagtc gccgcccaac cgcgcgagcg tgtcggtgcg gcgcagggta cgcctgaggc
      601 gcctgctcac ctccatgatg agccggtcgc cgtgccgctg gctgaacgtc tggttcacga
      661 agcggaagtt gtcgaggtcc aggatggcga gtgcgatgcc cgtgccgttg cgccgcgcgt
      721 gcagctgagc ctgttggacg cggtcctcga gcagatgggc gttcggcagc ccggtgagtg
      781 agtcgatgtg cgcgaggcgc cagaggttgg ccgtctcgcg tctggcgttg gttatgtcct
      841 tcaccatccc gtagacggcg gtgatgccgg cctcgtcgct gcgccagcgc cgggaggcga
      901 agtcctcgac ccacatgacc tttccgccgt acgttacgag gcggtactgg tccgaacggc
      961 gctcctggtc tcgcaggcct tgcaggcgct cttcgagctg cgccctgtcc tcggcgtgta
     1021 tgaagcgcct gaacccttgc gtcttgagat gctcgaccgc gtagccggtc aactggtgcc
     1081 aggcgtccga cacccacacg acctcgttgc gaccgtcgtc gtgcaagagc agcgcgtaac
     1141 tgaagaagcg gccgtcgccc tccagggacc ccgacagttc gagttgcatg gcctgcgccc
     1201 cgcctgcgtg gacttccgcc gtgacgtcgc ggaagagcgt caggacgtgg gtggccgcgt
     1261 cgccatcgac ggcgtggacg gggtagtggc gctgctcgac ccagagcagg ctaccgtccg
     1321 cccggtagat gcgcacgacg gacgtggacg gctcatcagc cgtggtcgcg tcgtacatac
     1381 gcccaccctc ctggtccgtg tcgttgcgca tgaggaaccc gaggctgcgg ccttggaact
     1441 cctcgaagga gtaacccgtc agctcgacca gcttgtcgga ggcgtgcacc acggggtgac
     1501 cgacggtcgc g
//
LOCUS       DAOQYE010000620         1510 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_104832_length_1510_cov_3.583505, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000620 DAOQYE010000000
VERSION     DAOQYE010000620.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104832_length_1510_cov_3.583505"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1190
                     /locus_tag="PKN52_09520"
     CDS             <1..1190
                     /locus_tag="PKN52_09520"
                     /inference="COORDINATES: protein motif:HMM:NF015185.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNR00231.1"
                     /translation="TQIALAGSMPLLAQVASPFGAWVAEVIGRRKVVAATLAAIGRAS
                     WIIAALLPTLAVPDAARPALLVALVFFAMTFQAATATVWTAWMGDVVPEERRGSYFGM
                     RAGVLGVVGMAANLAAGAFLDRVAAPLSFQVVMGVAILSSLIAVVLLLFHYDPPTERR
                     AVGLGELFRVPLADKNFRRFLMFAIYWQFAVMLGGPFVVPYWLEDLHMTFTQIAYWST
                     INAVTALLSTSQWGRVADRIGNKPVLAVGTFLAGALLPTTWILAGLTGDLTYIWLSAF
                     FDAIAWGAIGPSVFSLALGAAPRSRRVMFIAMYSLVQGVAGFVGGALAGPLLGFLQGL
                     PAPGWLPAWSGYHSLFVVTGVGRMLAWIFVRRVAEPRAWRTRDLLRAGRPVGAHGAGR
                     RRP"
BASE COUNT          178 a          546 c          512 g          274 t
ORIGIN      
        1 cgacgcagat agccctggcg ggcagcatgc cgctcctcgc gcaggtggcg agcccattcg
       61 gcgcctgggt agccgaggtc atcggcaggc gcaaggtcgt cgctgcgacc ctggcggcca
      121 tcggcagggc gagttggatc atcgcggcac tcctgcccac tctcgccgtg ccggacgcgg
      181 cccgccccgc tctgctcgtc gccctcgtct tcttcgccat gaccttccag gccgccaccg
      241 cgacggtgtg gacggcgtgg atgggcgacg tcgtgccgga ggagcggcgc ggcagctact
      301 tcggcatgcg cgccggcgtc ctcggcgtgg tcggcatggc cgccaacttg gccgctggcg
      361 cgttcctcga ccgggtggcg gccccgctct ccttccaggt cgtcatgggc gtggcgatcc
      421 tctcctcgct catcgccgtc gtcctcctcc ttttccacta cgacccgccg actgagcgcc
      481 gcgcggtggg gctcggcgaa ctgttccgag tgcccctcgc ggacaagaac ttccggcgtt
      541 tcctcatgtt cgcgatctac tggcagttcg ccgtgatgct cggcggaccg ttcgtggtgc
      601 cgtactggct cgaagacctg cacatgacgt tcacgcagat cgcctactgg agcaccatca
      661 acgccgtcac cgcgctgctg agcacgtcgc agtggggtcg tgtggccgac cgcatcggca
      721 acaagccggt cctcgccgtc ggaacgttcc tcgccggtgc gctcctgccg acgacctgga
      781 tcctcgcagg gttgacgggc gacctgacct acatctggct ctccgccttc ttcgacgcca
      841 tcgcttgggg cgccatcggc ccgtccgtct tcagcctggc gctcggcgcc gccccgaggt
      901 cgcgccgcgt gatgttcatc gccatgtact cgctcgtgca aggggtggcc ggcttcgtgg
      961 gcggcgcgct cgccggtccg ctcctcggtt tcctgcaagg cctcccggct ccgggctggc
     1021 tgccggcgtg gtctggatat cacagcctgt tcgtggtgac cggcgtcgga cggatgctcg
     1081 cgtggatctt cgtgcgccgc gtggcggagc cacgcgcttg gcgcacccgc gacctcctcc
     1141 gcgccggacg tccggtcggg gcgcacggcg ccggcaggag aaggccgtag tcgtcggact
     1201 cggtacgccg gcggcaccgg ggtagggccg ggggcgaacg ggtttggcgt tctcaggcca
     1261 tgtccgcgcc ggtggcccgc tgtgtacctg ccgtttccgg ccgaggcttg actagcgccg
     1321 gtcctcgcca taacctgctt acgtaaggac tttgcaaaac cggctttagg aggcggggtg
     1381 gaactcagga gcgtgcaaca cgacaccgtg cgcgagcaca accgcctcgc cgtgctcgag
     1441 gcgctcctgt cgcgccgtgt ggccgcgcgc tcggaactct ccagggcgac ccgcctcagc
     1501 gtccccaccg
//
LOCUS       DAOQYE010000621         1510 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_104897_length_1510_cov_2.021993, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000621 DAOQYE010000000
VERSION     DAOQYE010000621.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104897_length_1510_cov_2.021993"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="PKN52_09525"
     CDS             <1..556
                     /locus_tag="PKN52_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HNR00232.1"
                     /translation="AWAHNRLAARGEQRRILMVISDGAPVDDSTLSVNPGNYLEQHLR
                     EVNPGRRVIQDRTVYEDAAIFARLLHDDGVMDDRDHASYRTLYHAVLAALRPPDLLIY
                     LRAGLGTLKRHIAQRGRAYEANIEDGYLLRLGDLYERWVEDYSLSPVVVIPADDLDFV
                     NDQADLKKVLDLLERSGLTAPVVR"
     gene            688..>1510
                     /locus_tag="PKN52_09530"
     CDS             688..>1510
                     /locus_tag="PKN52_09530"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HNR00233.1"
                     /translation="MPTVEATPITAVVQDHRLVRPGAMFVARRGAKFDAHALLPAAAR
                     GGAGVLVGELPWAELPLADPAEQPAAPYLRVPNTRAALPHLAAAFERHPSSRLRVLGV
                     TGTDGKTTTSYLLHWLLLEDGAAALLSTAGIRLGEAELEPLSGHFTTPEATEVQALLG
                     RFLRGGASRVVLESSSHGFSLHRLDAVEYTVGVVTNLTPEHLDHHGTLDEYRDAKATL
                     VRRAATAVVNLDDEHAPYFLAEARAAGRKTVTFGASQGADVRIGRVTSMPGRLEFE"
BASE COUNT          223 a          563 c          507 g          217 t
ORIGIN      
        1 cgcctgggcg cacaaccgtc tggccgcacg cggcgagcag cggcgcatcc tgatggtgat
       61 ctccgacggc gcgccggtcg acgattcaac gctgtcggtg aatccgggca actacctcga
      121 gcagcacctg cgcgaggtca acccgggcag gcgcgtgatc caggacagga ccgtgtacga
      181 ggacgcggcc atcttcgcca ggctgctcca cgacgacggc gtgatggacg accgcgacca
      241 cgcctcttat cgcaccctct accacgcggt gctggccgcc ctccgccctc ccgacctcct
      301 catctacctg cgggccggct tgggcacgct caagcgccat atcgcgcagc gggggcgggc
      361 ttacgaggcg aacatcgagg acggctacct gttgcgcctg ggcgacctct acgagcgctg
      421 ggtggaggac tactccctct cgcccgtcgt ggtgatcccg gcagacgatc tcgacttcgt
      481 caacgaccag gccgacctca agaaggtgct cgacctgctg gagcggagcg ggctgacggc
      541 cccggtcgtc aggtgaaccg gtcgcccctc tgggacgacg gtcccggacc cctctccctc
      601 ggcgagttgt ggcgcggcgc gctcgcgacg ggcccctcgt cggttccgcc gctggaatcg
      661 gctatacaga ccgataagtc tattgatatg ccgacggtcg aggcgacgcc gatcaccgcc
      721 gtcgtccaag atcaccgcct ggtgaggccc ggcgccatgt tcgtggcgcg gcgcggggcc
      781 aagttcgacg cccacgccct actgcccgcg gcggcccgcg gcggcgcggg cgtgctcgta
      841 ggcgagctgc cgtgggcgga gctacctttg gcagaccccg ccgaacagcc ggccgcccct
      901 tacctcaggg ttcccaacac gcgcgccgcg ctcccccacc tggccgcggc gttcgagcgc
      961 cacccctcct cgcgcctacg ggtgctcggc gtgacgggga ccgacggcaa gacgacgacg
     1021 agctacctcc tgcactggct cctgctcgaa gacggcgcgg ccgcgctcct cagcacggcc
     1081 ggaatacgcc ttggcgaggc ggagctggag ccgctgagcg gccacttcac caccccggag
     1141 gccaccgagg tgcaggcgct cctcgggcgt ttcctcaggg gcggtgcgag ccgcgtcgtg
     1201 ttggagtcga gttcgcacgg cttctcgctc caccggctcg acgccgtgga gtacaccgtc
     1261 ggcgtggtca ccaacctgac ccccgagcac ctcgaccacc acggcacctt ggacgagtac
     1321 cgcgacgcga aggccacgct cgtgcgccgc gcggcaacgg ccgtggtgaa cctcgacgac
     1381 gagcacgccc cctacttcct cgcggaggcg cgcgcggccg gccgcaagac ggtcacgttc
     1441 ggcgcttcgc agggcgcgga cgtgcgcatc ggccgggtga ccagcatgcc cggcaggctc
     1501 gagttcgagc
//
LOCUS       DAOQYE010000622         1507 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_105197_length_1507_cov_2.679063, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000622 DAOQYE010000000
VERSION     DAOQYE010000622.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105197_length_1507_cov_2.679063"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1018)
                     /locus_tag="PKN52_09535"
     CDS             complement(<1..1018)
                     /locus_tag="PKN52_09535"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNR00234.1"
                     /translation="MKKAALALALILAFGTALAQTLVYGVSGYPTSLDAIDTTDGNSL
                     VVSSQISERLIDFAPGSTELVPALATSWSANDDATVWTFELRQGVKFHDGTPFNAEAV
                     KFNIDRLNHADNPAGNRAEGKTFVMWDDTFGGTIEGGSNLVADVEADGEYTVRMNLTR
                     PAPFLPALWAAVYFQFGSPAAIQAAGVGYGTPGYGAVGTGPFKFVEWVEGERVVLERN
                     DDYWNGPAGVERVIFRGVQEPTARLAELQAGTIDVAVLLTSDDLATVQADPNLVVQTA
                     DSELNVGYVAIHIDHSPLDNVLVRRAIMHAVDRDAIVDAFYEGLGVTASDLLPPVMFG
                     HGEPW"
     gene            1213..>1507
                     /locus_tag="PKN52_09540"
     CDS             1213..>1507
                     /locus_tag="PKN52_09540"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNR00235.1"
                     /translation="MGTEQMVELWLIRHGETEWNRQGRIQGHSDNPLNDLGRAQARAL
                     GGRLAVVRFDRIYSSDLGRAIETARLVFPDRAIETDVRLREVSAGVLEGKVSSE"
BASE COUNT          225 a          479 c          568 g          235 t
ORIGIN      
        1 gccacggctc gccgtgaccg aacatgacgg gcgggaggag gtcggacgcc gtgacgccca
       61 ggccctcgta gaaggcgtcg acgatggcgt cgcggtcgac cgcgtgcatg atggcgcggc
      121 gcacgagcac gttgtcgagg ggcgagtggt cgatatggat ggccacgtag cccacgttca
      181 gctcggagtc ggccgtctgg acgacgaggt tggggtcggc ctgaacggtg gcgaggtcgt
      241 ccgaggtcag gaggacggcg acgtcgatgg tgccggcctg cagctcggcg aggcgggcgg
      301 tcggctcctg cacgccgcgg aagatgacgc gctcgacgcc ggccggcccg ttccagtagt
      361 cgtcgttgcg ctcgagcacg acgcgctcac cctcgaccca ctcgacgaac ttgaagggcc
      421 cggtgccgac ggcaccgtag ccgggcgtgc cgtagccgac gccggcggcc tggatggcgg
      481 ccgggctgcc gaactggaag tagacggccg cccacagcgc gggcaggaac ggcgccgggc
      541 gggtgaggtt catgcggacc gtgtactcgc catcggcctc cacgtcggcg acgaggttgg
      601 agccgccctc gatggtgccg ccgaacgtgt cgtcccacat gacgaacgtc ttgccctcgg
      661 cgcggttgcc ggccgggttg tccgcgtggt tgaggcggtc gatgttgaac ttgacggcct
      721 cggcgttgaa cggcgtgccg tcgtggaact tgacgccctg acggagctcg aacgtccaga
      781 ccgtggcgtc gtcgttggca ctccacgagg tggcgagggc cggcaccagt tcggtggagc
      841 ccggcgcgaa gtcgatgagg cgctcggaga tctgtgagct gaccaccagc gagttgccgt
      901 cggtcgtgtc gatggcgtcg agcgacgtcg ggtagccgga gacgccgtac acgagcgtct
      961 gcgcgagcgc cgttccgaac gccagtatga gtgccagtgc caaagctgct ttcttcatcc
     1021 tgcctcctgc ggtgggtggc ttggagcagt gacccccgac catcgggtcg gatgtcgccg
     1081 gccaggtgct cgaagtcgcc ttcacctgtt ggttaggcac tcaggatacc acggcgtttc
     1141 ccgcgccgcc ggcgcccgcc gcgcgacggg gcacaaggtg ggcgccggct cgggcgcgtt
     1201 acactgcgcc cggtgggaac cgaacagatg gtggagctgt ggctgatacg ccacggcgag
     1261 acggagtgga accggcaggg gcggatccag gggcatagcg acaacccgct caacgacctc
     1321 ggaagggctc aggctcgcgc cttgggcggc aggctggccg tcgtgcggtt cgaccgcatc
     1381 tactcctccg accttggcag agcgatcgag actgccaggc tcgtcttccc cgaccgcgcc
     1441 atcgagaccg acgtgcggct gcgcgaggtg tcggccgggg tgctcgaggg caaggtctcc
     1501 tccgagc
//
LOCUS       DAOQYE010000623         1506 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_105304_length_1506_cov_2.889042, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000623 DAOQYE010000000
VERSION     DAOQYE010000623.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105304_length_1506_cov_2.889042"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(109..>1506)
                     /locus_tag="PKN52_09545"
     CDS             complement(109..>1506)
                     /locus_tag="PKN52_09545"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNR00236.1"
                     /translation="AEALKLTVSYVADEKVLVFSDGLRTARFKATSDIAAGLIKAPGT
                     VTLSPPVGGQTTLASPLAILVDGVAYVAITPLVAAFEGVSSWNAERHIITIDTADRLG
                     YTLARARTGLTDGVSRVAIDIPENADYQVAAGGKAFVIVLPGARSESYDVAVDDPNLS
                     AVGVTAEAGRVTIGVHTKFDLEASGSGFRVGIVPKATGRTLYVDFAPSVRGNAVTALQ
                     PAPGAGQTEPAIQEALQVVPEHRQVVVIDAGHGGHDPGATGFAIEKQIVLSVAMKLKQ
                     LLEAEGVDVILTRGDDTFLTLQQRSLFATPERNVFVSIHANSAANASASGIETWVFGE
                     PLNPSLIDRAIEENGGGTEGQALTEEARASARDLAADILREAQLNYSLTLAETVQRNL
                     IDATGARDRGVRANLFYVIRTARIPAILVELGFVSNPEEGRDLATDSYQGKLAKALAD
                     GLLEFLHGGGLVARR"
BASE COUNT          226 a          488 c          543 g          249 t
ORIGIN      
        1 gtcgtgatag tggcgggaca tgccccaaca tgctacaccg ctaccagaaa ggcgctcagg
       61 gagccggtcg gggtccgcga cgcccgcacc ccgggccccg tcagggcatc agcgcctggc
      121 gaccagcccg ccgccgtgta agaactcgag gaggccatcg gccagcgctt tggcgagctt
      181 gccctggtaa ctgtccgtgg ccaagtcgcg accttcctcg gggttgctga cgaaaccgag
      241 ctccacgagg atggccggga tgcgcgccgt gcggatcacg tagaacaggt tggctctcac
      301 gccgcggtcg cgggctcccg tggcgtctat gaggttgcgt tgcacggtct cggccagagt
      361 gagcgagtag ttgagttgcg cctcgcgcag gatgtccgcc gcgaggtcgc gtgcgctggc
      421 gcgcgcctcc tccgtgagag cctggccctc ggtgccgccg ccgttctcct cgatggccct
      481 gtcgatcagg gaaggattca gcggctctcc gaacacccag gtctcgatgc cggaagccga
      541 ggcgttggcg gccgagttcg cgtggatcga gacgaagacg ttgcgttccg gcgtggcgaa
      601 gagggagcgc tgttggaggg tgaggaacgt gtcgtcaccc cgcgtgagga tgacgtcgac
      661 cccctcggcc tcgaggagct gcttgagctt catcgcgacg gagagcacga tctgtttctc
      721 gatggcgaag ccagtcgccc ccggatcgtg gccgccgtgg ccggcgtcga tgaccacgac
      781 ctgccggtgc tccggcacga cctggagcgc ctcctgtata gccggctcgg tctgcccggc
      841 tccaggggcc ggctggagcg ccgtcacggc gttgccgcgt acgctcggcg cgaagtcgac
      901 gtacaacgtg cggccggtcg ctttcggcac gatgccgacg cggaagccgc taccggaagc
      961 ctcgaggtcg aacttggtgt ggacgccgat cgtgacgcgc ccagcctccg ccgtgacgcc
     1021 gaccgcgctg aggttgggat cgtcgaccgc cacgtcgtag gattccgagc gcgcgcccgg
     1081 caggacgatc acgaaggcct tcccgccggc cgcgacctgg tagtcggcgt tctccgggat
     1141 gtcgatggcg acgcgcgaga cgccgtcggt caggccggtc cgcgcccgtg cgagcgtgta
     1201 gccgagacgg tcggccgtgt cgatggtgat gatgtgacgt tcggcgttcc acgagctcac
     1261 gccctcgaag gccgcgacga gcggagttat ggccacgtac gcgaccccgt cgacgaggat
     1321 ggcaagcggg ctcgccagag tggtctgacc gccgaccggc ggggagaggg tgacggtgcc
     1381 cggcgccttg atcaagccgg ccgcgatatc ggacgtggcc ttgaagcgcg ccgtcctgag
     1441 gccgtctgag aagacgagga ctttctcgtc tgcgacgtag ctgacggtta gcttcaaggc
     1501 ttctgc
//
LOCUS       DAOQYE010000624         1505 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_105460_length_1505_cov_2.542759, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000624 DAOQYE010000000
VERSION     DAOQYE010000624.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105460_length_1505_cov_2.542759"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            64..534
                     /gene="rpsE"
                     /locus_tag="PKN52_09550"
     CDS             64..534
                     /gene="rpsE"
                     /locus_tag="PKN52_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HNR00237.1"
                     /translation="MADTDFEDKVIFIRGTAKTYKGGRRFRFGAMVVIGDRQGRVGVG
                     LGKAREVPVAVQKGQFVARRNIINVPIQEPGTVPQVLGEHGTSRVMLKPAGAGTGVIA
                     GSVPRAIVELAGYSNLLTKELGSRNQTNVAYAVIDGLKQLRTFAEAKRVRESAE"
     gene            531..707
                     /gene="rpmD"
                     /locus_tag="PKN52_09555"
     CDS             531..707
                     /gene="rpmD"
                     /locus_tag="PKN52_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HNR00238.1"
                     /translation="MKVKLVRSLIGTTKVQRGTVNALGLKKIGDVREVVDNESTRGML
                     AKVKHLLTIEGEKR"
     gene            704..1162
                     /gene="rplO"
                     /locus_tag="PKN52_09560"
     CDS             704..1162
                     /gene="rplO"
                     /locus_tag="PKN52_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015539819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HNR00239.1"
                     /translation="MKLNELSPAPGSKKTRKRVGRGLGSGRGKTAGRGQKGQTSRSGH
                     SQGAGWEGGRSRLIMRLPKRGFTRVKEPLQLVNLRDLNAFASGEAIDAAKLVANGLIR
                     RVDHRVKLLADGDLEVKGLTITVDAASKGALAAVEAAGGKVVLTSAGEGA"
     gene            1165..>1505
                     /locus_tag="PKN52_09565"
     CDS             1165..>1505
                     /locus_tag="PKN52_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HNR00240.1"
                     /translation="MLKAFRNALVIPDLRAKLLVTVGLLAAYRLMVHIPTPGVDVQAL
                     KAGALGGGVFSLLNFISGGNFEVFSIAALGVIPYITASIIIQLLQSTYPPLEKLSKEG
                     EEGRRKITQYTR"
BASE COUNT          271 a          530 c          498 g          206 t
ORIGIN      
        1 caggtaccac ggtcgcgtca aggccctcgc tgaaggggcc cgtgaaggag gcctcgagtt
       61 ctgatggctg ataccgactt cgaagacaag gtcatcttca tccggggcac cgccaagacg
      121 tacaagggcg gcaggcgctt ccggttcggg gccatggtcg tcatcggcga ccgtcaggga
      181 cgcgtcggcg tcggcctcgg caaggctcgc gaggtccccg tggccgtgca gaagggccag
      241 ttcgtcgcgc gccgcaacat catcaacgtg cccatccagg agcccggcac cgtcccccag
      301 gtcctgggcg agcacggcac cagccgcgtg atgctcaagc cggccggcgc cggcaccggc
      361 gtcatcgccg gcagcgtgcc gcgcgccatc gtcgagctgg ccggctacag caacctcctc
      421 accaaggagc tcggcagccg caaccagacg aacgtggcct acgcggtcat cgacggcctc
      481 aagcagctcc gcaccttcgc cgaagccaag cgagtgaggg agtcggcgga atgaaggtca
      541 agctcgtccg cagcctgatc ggcacgacca aggtccagcg cggcacggtc aacgccctcg
      601 ggctcaagaa gatcggcgac gtgcgcgagg tcgtcgacaa cgagtcgacg cgcggcatgc
      661 tcgcgaaggt caagcacctg ctcaccatcg aaggggagaa gcggtgaagc tcaacgaact
      721 aagcccggca ccgggctcga agaagacgcg caagcgcgtc ggccgcggcc tcgggagcgg
      781 tcgcggcaag acggcgggcc ggggtcagaa gggccagacc agccgttcag gccatagcca
      841 aggggccggc tgggagggcg gacgctcccg cctgatcatg cgcctgccga agcgcggctt
      901 cacgcgcgtc aaggagccct tgcaactggt caacctgcgt gacctcaacg ccttcgcctc
      961 cggcgaggcc atcgacgccg ccaagctcgt ggccaacggc ctgatccgcc gggtcgacca
     1021 ccgcgtcaag ctgctggccg acggcgacct cgaggtgaag ggcctcacca tcaccgtcga
     1081 tgccgcctcc aagggcgccc tcgccgcggt ggaagccgcc ggcggcaagg tcgtcctgac
     1141 ctcggccggc gagggagcct aagcatgctt aaggccttcc gcaatgctct ggtcatcccg
     1201 gacttgcggg ccaagctgct cgtcaccgtc ggcctgctgg ccgcctaccg cctgatggtc
     1261 cacatcccga caccgggcgt ggacgtccag gcgttgaagg ccggcgccct cggtggcggc
     1321 gtcttctccc tgctcaactt catctcgggc ggcaacttcg aggtcttctc gatcgccgcg
     1381 ctcggcgtga tcccgtacat cacggcgagc atcatcatcc agctcctgca gagcacctac
     1441 ccgcccctcg agaagctctc gaaggaaggc gaggagggcc ggcgcaagat cacgcagtac
     1501 acgcg
//
LOCUS       DAOQYE010000625         1503 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_105663_length_1503_cov_3.081492, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000625 DAOQYE010000000
VERSION     DAOQYE010000625.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105663_length_1503_cov_3.081492"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1044
                     /locus_tag="PKN52_09570"
     CDS             <1..1044
                     /locus_tag="PKN52_09570"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HNR00241.1"
                     /translation="YCTSIAPGDQVFVQVDAGALELARAITRRVLRAGAEPHLRLLYP
                     EQTADLLELATDELLASEPATLLAEMKRMDAYIRVNAPTNAYELEGADQARQAALGKR
                     LRDVTNQRTRHTRWVGTLYPTPGAAQVAGMSSEAYERFVFGAMFLDDPDPAARWRELG
                     AAQQRWADRLSQAREVRITGPGTDLRLDVTGRKWVNSDGKRNMPSGEVFTGPIESSAE
                     GVITFEVPSSVAGTIVEGVTLRFEGGRVVEASARRGQELLDARLEADAGARYLGELGI
                     GTNPHITMPTLQTLFDEKILGTVHLALGSSYPETGGKNESSIHWDLICDLRGGGEVTL
                     DGETFLQDGKLLG"
     gene            1149..1235
                     /locus_tag="PKN52_09575"
     tRNA            1149..1235
                     /locus_tag="PKN52_09575"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1183..1185,aa:Leu,seq:cag)
     gene            complement(1322..>1503)
                     /locus_tag="PKN52_09580"
     CDS             complement(1322..>1503)
                     /locus_tag="PKN52_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00242.1"
                     /translation="GLEGAMQEVVSLKAPLYEFLDSVHVLSDDLDVRRNRLTLLGEVV
                     RILRQLGSLEQLAVG"
BASE COUNT          234 a          541 c          522 g          206 t
ORIGIN      
        1 tactgcacga gcatcgcccc tggggaccag gtgttcgtgc aggtcgacgc cggcgcgctc
       61 gaactggcgc gcgccatcac gcggcgcgtc ctgcgcgccg gggccgagcc gcacctccgc
      121 ctcctctacc ccgagcagac cgccgacctg ctcgagttgg ccactgacga gctcctggcc
      181 tccgagcccg ccaccctcct agccgagatg aagcgtatgg acgcctacat ccgggtgaac
      241 gccccgacga acgcctacga gttggagggt gcggaccagg cccgtcaggc ggcgctcggc
      301 aagcggctgc gcgacgtcac caaccagcgt acgcgccaca cgcgctgggt cggcaccctc
      361 tatccgacgc cgggcgccgc gcaggtggcc ggtatgagca gcgaggcgta cgagcggttc
      421 gtcttcggcg ccatgttcct tgacgacccc gatccggccg cgcggtggcg ggaactcggc
      481 gcggcgcagc agcgctgggc ggaccgcctc tcgcaagcgc gcgaggtgcg catcaccggt
      541 cccggcacgg acttgcgcct ggacgtgacg ggccgcaagt gggtcaactc cgacggcaag
      601 cgcaacatgc cttccggcga ggtgttcacg gggcccatcg agtcctccgc cgagggcgtg
      661 atcacgttcg aggtcccgtc gagcgtggcc ggcacgatcg tcgagggcgt cacgctgcgc
      721 ttcgagggcg gtcgggtcgt ggaagcgagc gcgcggcgtg gccaggagct gctcgacgcg
      781 cggctcgagg ccgacgccgg ggcgcgttac ctgggcgagc tcggcatcgg caccaacccg
      841 cacatcacca tgcccaccct acagacgctc ttcgacgaga agatcctcgg gacggtacac
      901 ctggccctcg gctcctcata tccggagacg ggcggcaaga acgagagcag catccactgg
      961 gacctgatct gcgacctgcg gggcggcggc gaggtgacgc tcgacggcga gacgttcctg
     1021 caggacggca agctgctggg ctgaacgccg cccgccccac cccttgcgcg ccgtctcctg
     1081 cgtgttacta tcagcacccg cggcggctcg aagccgcggc tggtcaaagc cgcgccggct
     1141 gggcgcgcgc cgaagtggcg gaatcggtag acgcgcatga ttcagggtca tgtgtccgca
     1201 aggacgtgtg ggttcaagtc ccaccttcgg caccaaccgc tgcgcccagc acctcgaacg
     1261 ccatatggca ggccccgggt tagcgcccgg ggcctgcgca tgcgcttgga cgccgcgcgg
     1321 ctcagccgac cgcgagctgc tccaggctcc cgagctgacg caggatccgc acgacctcgc
     1381 cgagcagggt caagcggttg cggcgcacgt ccaggtcgtc cgagagcacg tgcaccgagt
     1441 cgaggaactc gtagagcggc gccttgaggc tcacgacctc ctgcatggcg ccctcgaggc
     1501 cgc
//
LOCUS       DAOQYE010000626         1502 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_105798_length_1502_cov_2.432619, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000626 DAOQYE010000000
VERSION     DAOQYE010000626.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105798_length_1502_cov_2.432619"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1450
                     /locus_tag="PKN52_09585"
     CDS             <1..1450
                     /locus_tag="PKN52_09585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNR00243.1"
                     /translation="ANALYEKAARGLVKELNDPYTELFSPQQMAEFTRNTNGRYAGLG
                     MEITPIGAYVTVGKVFPETPAERGGVMEGDRILQIDTFPARGWTTQQVQNKLLGLPGT
                     QVRVTFGRPGVAEPITLNFRRAEVKVPAVRYTLMIDERTGYIPIERFSENTTDDVAKA
                     VRTLQAQGAKGIILDVRGNPGGILDQAIATANLFLPAGKEVASVRGRGDEQHFTTDRD
                     PLAADIPLVVLTDGGSASASEIVAGALQDYDRALVLGTTSFGKGLVQSLYPLDGGYAL
                     KITTGKWFTPSGRSIQRDRKQGDAFVLEAVADTAVVDTAVSHRPTYKSVSGRTLYGGG
                     GITPDVMVRPDTISSDEQAVARLILPKTGQFFSVLGELAIELRDKVQPDFELQSAWVE
                     DFYTRLDTAGVKVDREVWDKGRRWTDRYLENRIARMAYGDTLAKRREIRDDNQLRKAV
                     ELLRKGNTQSELFAAARAEAARPARGGANRGN"
BASE COUNT          253 a          581 c          467 g          201 t
ORIGIN      
        1 cgccaacgcg ctgtacgaga aggccgcccg cggcctcgtg aaggagctca acgacccgta
       61 caccgagctc ttctcgccgc agcagatggc ggagttcacg cgcaacacca acggtcgcta
      121 cgccggcctg ggcatggaga tcacgcccat cggcgcttac gtcaccgtgg gcaaggtctt
      181 ccccgagacg cccgccgagc gcggcggcgt catggagggc gaccgcatcc tgcagatcga
      241 caccttcccg gcccgcggct ggaccacgca gcaggtgcag aacaagctgc tcggcctccc
      301 cggcacccag gtgcgcgtca ccttcggccg ccccggcgtc gccgagccca tcacgctcaa
      361 cttccgccgc gccgaagtga aggtgcccgc cgtgcgctac acgctcatga tcgacgagcg
      421 caccggctac atcccgatcg aacgcttcag cgagaacacc accgacgacg ttgccaaggc
      481 cgtccgtacc ttgcaggcgc agggtgcgaa gggcatcatc ctcgacgtgc gcggcaaccc
      541 ggggggcatc ctcgaccagg ccatcgccac cgccaacctc ttcctgcccg ccggcaagga
      601 agtggccagc gtgcgcggcc gcggcgacga acagcacttc accaccgacc gcgatcccct
      661 cgccgccgac atcccgctcg tcgtgctcac cgacggcggt tccgcctccg cgtccgagat
      721 cgtggccggc gcgctgcagg actacgaccg cgccctcgtg cttggcacca cctcgttcgg
      781 caagggactc gtccagtccc tctatccgct cgacggcggc tacgccctca agatcaccac
      841 gggcaagtgg ttcacgccga gtggtcgctc catccagagg gatcgcaagc agggcgacgc
      901 gttcgtcctc gaggcggtcg ccgacacggc cgtggtcgat acggcggtct cccatcgccc
      961 gacctacaag tcggtcagcg gacgcaccct gtacggcggc ggtggcatca cccccgacgt
     1021 catggtgcgc cccgacacca tctccagcga tgaacaggcg gtcgcccgcc tcatcctgcc
     1081 caagacgggc cagttcttct ccgtgctcgg cgaactcgcc atcgagctgc gcgacaaggt
     1141 gcagcccgac ttcgagctgc agagcgcctg ggtcgaggac ttctacacgc gcctcgacac
     1201 ggccggcgtg aaggtcgacc gtgaggtctg ggacaagggc cggcgctgga ccgaccgcta
     1261 cctcgagaac cgcatcgccc gcatggccta cggcgacacc ctggccaagc gccgcgagat
     1321 ccgcgacgac aaccagctcc gcaaggccgt cgagctgctc cgcaagggca acacccagtc
     1381 ggagctcttt gccgccgccc gggcggaggc cgcgcgcccc gcgcgcggcg gcgccaaccg
     1441 cggcaactga ggccctcggg tccggaattg tacggactgc cccgggcgtc ccgtcccggc
     1501 ta
//
LOCUS       DAOQYE010000627         1499 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_106137_length_1499_cov_3.347645, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000627 DAOQYE010000000
VERSION     DAOQYE010000627.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1499
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106137_length_1499_cov_3.347645"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(43..879)
                     /locus_tag="PKN52_09590"
     CDS             complement(43..879)
                     /locus_tag="PKN52_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00244.1"
                     /translation="MAEFSATEERVVEERPTEEQFLVAQEHFSGSLGELAHALRTGSL
                     EPRRIDLYRLVRDYLVYFERHAEADLELATEALPRLAQVIELKTRLLLPKPPKVPEEE
                     DEEAVAIALEAVALLEELEEAILFLRRRREERRLVVPARAPAPDYPRKERPLRVTPRD
                     LARLAGRYRVGGYFELALERLTLASMGRFILRTVRGLGSGRLWDILGARDWPTRTVGL
                     AAVLELVREGRLSASQEEPFGPIVVTAARGGVDVGRAAAEDDWTEPESDLAIGSFEAP
                     AA"
     gene            1001..>1499
                     /locus_tag="PKN52_09595"
     CDS             1001..>1499
                     /locus_tag="PKN52_09595"
                     /inference="COORDINATES: protein motif:HMM:NF013355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase (quinone)"
                     /protein_id="HNR00245.1"
                     /translation="MNAYDLAKPYLFRLDPERAHDLVIRALALASRSAVAPRLLEAFA
                     PPHDPRLEVELFGLKFPNPLGLAAGLDKNGVAFPALAALGFGAVEVGSVTALAQPGNP
                     QPRLFRLVEDEALVNRMGFNNAGAAAMAARLKRLDRGELRGRPARAVLGINVGKSRAV
                     DVAAAG"
BASE COUNT          215 a          576 c          506 g          202 t
ORIGIN      
        1 acatgcgcaa gcgtgagcgg ggcaaggccc tggcctgagc cttcaggcag ctggcgcctc
       61 gaacgacccg atcgcgaggt cgctttccgg ctccgtccaa tcgtcttccg ccgcagcccg
      121 cccgacgtct acgccgcccc gcgccgccgt gaccacgatc ggcccgaacg gctcctcctg
      181 cgaggcgctc agtcggccct cgcgcacgag ttcgaggacg gcggccagac cgaccgtccg
      241 cgtcggccag tcgcgggcgc ccaggatgtc ccagaggcgg ccgctcccga ggcccctcac
      301 ggtccggagg atgaaacgcc ccatgctggc gagggtcagg cgctccagcg cgagttcgaa
      361 gtagccgccg acacggtagc ggcccgcgag gcgggcgagg tcgcgcgggg tgacccgcag
      421 gggccgttcc ttgcgcgggt agtcgggtgc cggcgcgcgc gccggcacca ccagccggcg
      481 ctcctcgcgg cggcggcgca ggaagaggat cgcctcttcc aactcctcca ggagcgccac
      541 ggcctccagc gcgatggcca cggcttcctc gtcttcctcc tccggcacct tcggcggctt
      601 cgggagcagc aggcgggtct tgagctcgat gacctgcgcc aggcgcggca acgcctcggt
      661 agccaactcc aggtcggcct cggcgtgacg ctcgaagtac accaggtagt cgcggaccag
      721 tcggtagagg tctatgcggc ggggctcgag cgagccggtc ctaagcgcgt gggcgagctc
      781 gccgagcgag ccggagaagt gttcctgggc cacgaggaac tgctcctcgg ttggcctctc
      841 ttcgacgacc ctctcctctg tagccgagaa ctccgccacc ggccgacgtt accacggcac
      901 caaaaacgcc ccgctcgggc gtacggtccc atcaacgtcc accaaccgtc gccatgacgg
      961 cgctgcccca agcggcacac ccgtgcgaga ctgaaggcgc gtgaacgcct acgacctcgc
     1021 caagccctac ctgttccgcc tcgacccgga gagagcgcac gacctggtca tccgcgccct
     1081 ggcgctggcc agccgcagcg cggtcgcccc gcgcctcctg gaagcgttcg ccccgcccca
     1141 cgatccgcgc ctggaggtag agctcttcgg cctcaagttc ccgaacccgc tggggctggc
     1201 cgccggcctc gacaagaacg gcgtggcctt ccccgccctt gcggcgctcg gcttcggcgc
     1261 cgtcgaggtc ggcagcgtca ccgcgctcgc ccagccgggc aacccgcaac ccaggctctt
     1321 ccgcctcgtc gaggacgagg ccctggtaaa ccgcatgggc ttcaacaacg ccggggcggc
     1381 cgccatggcc gcgcggttga aacggctgga ccgcggcgag ctgcgcggcc ggccggcgcg
     1441 cgccgtcctc ggcatcaacg tcggcaagtc gcgtgccgtg gatgtcgccg cggccggcg
//
LOCUS       DAOQYE010000628         1497 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_106408_length_1497_cov_2.776699, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000628 DAOQYE010000000
VERSION     DAOQYE010000628.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1497
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106408_length_1497_cov_2.776699"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..519)
                     /locus_tag="PKN52_09600"
     CDS             complement(<1..519)
                     /locus_tag="PKN52_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase beta-grasp
                     domain-containing protein"
                     /protein_id="HNR00246.1"
                     /translation="MAKSRADIKQLLKEHDVRFLRLQFTDIHGRNKNVEVPTSQFDKA
                     LDGEIMFDGSSIKGFTRIEESDMLLSPDYDTFLLLPPAVEGSVPGQVARLICDINEPD
                     GTPFDGDPRGVLKRQLAKLRELGYDDLFVGVEPEFYLFKRGVDGSPTTITNDGAGYFD
                     LAPVDRGEDARRD"
     gene            834..>1497
                     /locus_tag="PKN52_09605"
     CDS             834..>1497
                     /locus_tag="PKN52_09605"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNR00247.1"
                     /translation="MHKKLVWLLVAALIGLAGAQTPPVSKVGVLLSYTGPLAEFGPAI
                     GNGVKLAADQINAAATAVFGGPIVEIVTEDDATAAAQGVERARKLVNADGVVAIVGSL
                     SSGVSVAVAESVAIPSNVVLISPASTSPLLAMLPDDGDVVYRTVASDALQGVVGAMLA
                     RGEIFSDHSYNTAAILYDNNPYGQGLADAFEAAFTKRGGTITAKVAHPDEPQPTYAAL
                     LEQ"
BASE COUNT          243 a          507 c          479 g          268 t
ORIGIN      
        1 gtcgcggcgc gcgtcctcgc cccggtcgac cggggcgagg tcgaagtagc ccgcgccgtc
       61 gttcgtgatg gtggtggggc tgccgtcgac gccgcgcttg aacaggtaga actccggctc
      121 gacgccgacg aacaggtcgt cgtacccgag ctcgcggagc ttcgcgagct ggcgcttcag
      181 caccccgcgc gggtcgccat cgaagggcgt gccgtccggt tcgttgatgt cgcagatgag
      241 gcgcgccacc tggccgggca cactgccctc caccgcgggc gggaggagga ggaacgtgtc
      301 gtaatcgggg ctgaggagca tgtctgactc ctcgatgcgc gtgaagccct tgatcgacga
      361 gccgtcgaac atgatctcgc cgtcgagcgc cttgtcgaac tgggaggttg gcacctctac
      421 gttcttgttg cgcccatgga tgtcggtgaa ctggagcctg aggaaccgga cgtcgtgttc
      481 cttcaacagc tgtttgatgt cggcgcggga cttggccaca cttcctcctg ggaaccgaaa
      541 agctcgggtc tcgatcgcta ccggacgacc gttgggtcga tcggcgcatc gcaacgaccg
      601 ggagaatagc acaatcctgc aggtcgtgat gaggaaacaa gacgaatgtg atgtttccgc
      661 atgacacttg gcaccgcccc gtggtcgcgg ccggtcgtaa ggcgtacgtg aggcccttaa
      721 cctttccttt ctccctgcca ccgtcgacgt tgacagccta tgtggggttt cttacgatgc
      781 aaccgaccgc cgcacgtaga catcgtcgcg tttttcgaac ctaggaggag aacgtgcata
      841 agaagctagt ttggttgttg gtggctgcgt tgatcggctt ggccggcgcg cagaccccgc
      901 ccgtctctaa ggtcggtgtg ctcctgtctt acaccggtcc gctggccgag ttcggtccgg
      961 ccatcggcaa cggcgtcaag ctcgccgccg accagatcaa cgccgccgca acggcggtct
     1021 tcggcggtcc gatcgtcgag atcgtcaccg aggacgacgc caccgctgcc gcgcagggcg
     1081 tcgaacgcgc ccgcaagctc gtcaacgccg acggcgtcgt cgccatcgtc ggctcgctgt
     1141 cgagcggcgt cagcgtggcg gtcgccgagt ccgtggccat cccgagcaac gtagtcctga
     1201 tctcgccggc ctccacctcg ccgctactcg cgatgctccc ggacgacggc gacgtcgtct
     1261 accgcaccgt ggcctccgac gcgctccagg gcgtcgtcgg cgccatgctc gcgcgcggtg
     1321 agatcttctc cgatcacagc tacaacaccg ccgccatcct gtacgacaac aacccgtacg
     1381 gccaaggctt ggccgacgcc ttcgaggccg ccttcaccaa gcgcggcggc accatcacgg
     1441 ccaaggtcgc ccacccggac gagccgcagc ccacgtacgc cgccctcctc gagcagg
//
LOCUS       DAOQYE010000629         1496 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_106545_length_1496_cov_2.231090, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000629 DAOQYE010000000
VERSION     DAOQYE010000629.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1496
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106545_length_1496_cov_2.231090"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(79..154)
                     /locus_tag="PKN52_09610"
     tRNA            complement(79..154)
                     /locus_tag="PKN52_09610"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(119..121),aa:Thr,seq:tgt)
     gene            348..611
                     /locus_tag="PKN52_09615"
     CDS             348..611
                     /locus_tag="PKN52_09615"
                     /inference="COORDINATES: protein motif:HMM:NF011430.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HNR00248.1"
                     /translation="MGRIGPIGIPELLIILALVLLIFGPKRLPEMAKGLGQSVREFRK
                     GLRDMKKDLDAESDDDSGKAAPVSAASAGVPAAKPAVGSEDAQ"
     gene            662..1414
                     /gene="tatC"
                     /locus_tag="PKN52_09620"
     CDS             662..1414
                     /gene="tatC"
                     /locus_tag="PKN52_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HNR00249.1"
                     /translation="MTLIEHFEELRKRLFIALVAWLVASGVAFVFRFDVLAWLKAPLP
                     PSMTLNYFAVLEPFTVSMQVASFFGLVLASPVIIGQVWGFVAPGLYAEERRYAVPFIL
                     FAALAFAAGVAFAYYVVLPFSIPILLSFLGDEAQGLLSIGRYISTLLMLMAMFGIMFE
                     LPVLGFLLARIGILRHAPLVRYRRWAIVGGVSAAAIITPTGDPFNLALVAVPFLVLYE
                     LTVIVVRISERRGDSQRRMRHAREDPEPTRSY"
     gene            1377..>1496
                     /locus_tag="PKN52_09625"
     CDS             1377..>1496
                     /locus_tag="PKN52_09625"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: protein motif:HMM:NF013939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HNR00250.1"
                     /translation="MQEKIQSLRARIDALNLKILELVSERGAVAAEIGALQSAM"
BASE COUNT          225 a          541 c          449 g          281 t
ORIGIN      
        1 gtccgtcccc tttaaccact cgggcaccta cccgcgccac taccggagtc tggcggcgct
       61 acccttcggc ccaaacagtg gagccaccca tcggaatcga accgacaacc ttccgattac
      121 aagtcgggtg ctctaccgat tgagctaggg tggccaaccg cgccacagac ccgaggtaac
      181 gtagcactag gtcgccagcc ctgtcaacta ccgtcctgga cgcgtcttgc gcctccccga
      241 cctgccctcg cgggggccgc gcagaacgtc ggtcgaccgg ccgggtccgt ccgtgcaccc
      301 gccccgtgcc cgcgcctgct aacatgctgg cgtttggagg cgccactatg ggacgcatag
      361 gacccatcgg gatcccggaa ctcctcatca tcctcgcgct agtactgctc atcttcggac
      421 ccaagcgctt gccagagatg gcgaagggct tgggccagtc cgtgcgcgag ttccgcaagg
      481 gcctgcgcga catgaagaag gacctcgacg ccgagagcga cgacgactcc ggcaaggccg
      541 cgcccgtctc ggccgccagc gccggcgtcc cggccgccaa gccggcggta ggcagcgagg
      601 atgcgcaata gctggcgcgg tagcccgcta gctagccctt agggacgacc agaggcttcg
      661 cttgaccctg atcgaacatt tcgaggagct acgcaagcgg ctcttcatcg ctctggtggc
      721 atggctcgtc gcttccggcg tcgctttcgt gttccggttc gacgtgttgg catggctcaa
      781 ggcgccgctc ccgccgtcca tgacgctcaa ctacttcgcc gtgctggagc cgttcaccgt
      841 ctccatgcag gtcgcgtcgt tcttcgggct ggtgctcgcc tcgccggtga tcatcgggca
      901 ggtctggggc ttcgtggcgc cgggactata cgcggaggaa cgccgctacg cagtgccgtt
      961 catcctgttc gcggccctcg cgttcgctgc cggcgtcgcc ttcgcgtact acgtcgtcct
     1021 ccccttcagc atccccatcc tcctatcgtt cctcggcgac gaggcgcagg ggctgctctc
     1081 gatcggacgc tacatctcca cgctcctgat gctgatggcg atgttcggga tcatgttcga
     1141 gctgcccgtg ctcggcttcc tgctcgcgcg catcgggatc ctgcggcacg cgccccttgt
     1201 caggtaccgc cgctgggcca tcgtcggcgg cgtgagcgcg gcggcgatca tcacccccac
     1261 cggcgacccc ttcaacctcg cgctcgtggc cgtacccttc cttgtcctct acgagctgac
     1321 ggtcatcgtc gtccgcatct cggaacgccg cggtgactcc cagcgtagga tgcgccatgc
     1381 aagagaagat ccagagccta cgcgctcgta ttgacgccct caacctgaag atccttgaac
     1441 tcgtctccga gcgcggcgcc gtagccgccg agatcggcgc cctgcagtcg gccatg
//
LOCUS       DAOQYE010000630         1495 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_106617_length_1495_cov_3.277083, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000630 DAOQYE010000000
VERSION     DAOQYE010000630.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1495
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106617_length_1495_cov_3.277083"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /locus_tag="PKN52_09630"
     CDS             complement(<1..625)
                     /locus_tag="PKN52_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013343673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HNR00251.1"
                     /translation="MRALSKLEPREGIWRVDVPEPTPGPNDLLIRIAKTSLCGTDVHI
                     YNWDAWSQRTVPVPLVTGHEWVGVVAGVGSEVSGFAVGDRVSGEGHITCGHCRNCRAG
                     RRHLCRNTVGVGVQRQGAFADYLVLPAFNAFRLPDAIPDDVGAILDPLGNAVHTALTY
                     DLVGEDVLITGAGPIGLMAAAVARFVGARNIVITDVRPARLSMAQRMG"
     gene            complement(622..>1495)
                     /locus_tag="PKN52_09635"
     CDS             complement(622..>1495)
                     /locus_tag="PKN52_09635"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF005394.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HNR00252.1"
                     /translation="FDANGGLFETLLGEEDAIISDALNHASIIDGVRLSKAARFRYAN
                     NDMADLERQLQAASGARFRLIATDGVFSMDGIIADLRAICDLADRYDALVMVDDSHAV
                     GFVGEGGRGSHEARGVMDRVDILTGTLGKALGGASGGYTSGRREIIDWLRQRSRPYLF
                     SNSIPPVVAATSLRVLDLLEDDSSSGGAELRARLRANSDHFRGAMTAAGFDLAGAGHP
                     IIPVMVYDAALAGRMADRLLEEGVYVVGFSFPVVPHGKARIRTQMSAAHTAEQLDMAV
                     EAFTRVGRELGVIA"
BASE COUNT          216 a          523 c          524 g          232 t
ORIGIN      
        1 cgcccatgcg ctgcgccatg gacagccggg ccggccttac gtccgtgatg acgatgttgc
       61 gcgcgccgac gaagcgcgcc accgcggcgg ccatcaggcc gatgggcccg gcgcccgtga
      121 tgagcacgtc ctcgccgacg aggtcgtacg tgagggccgt atggacggcg ttcccgagcg
      181 ggtcgaggat ggcgccgacg tcgtctggga tggcgtccgg gagcctgaag gcgttgaacg
      241 ccggcaggac gaggtagtcg gcgaacgccc cctggcgctg cacgcccacg cccacggtgt
      301 tgcggcagag atgcctgcgg ccggcccggc agttgcggca gtgaccgcat gtgatgtgcc
      361 cctcgccgct gacgcggtcg cccacggcga agccgctcac ttccgatccg acgcccgcga
      421 ccaccccgac ccactcgtgc ccggtgacga gcggcacggg caccgtccgc tgcgaccagg
      481 cgtcccagtt gtagatgtgg acgtcggtgc cgcagagact cgtcttggct atgcgtatca
      541 gcaggtcgtt tggtccaggc gtcggctccg ggacgtcgac ccgccagatg ccctcgcggg
      601 gctccagctt ggagagcgcc ctcacgcgat cacacccagt tcgcgcccga ccctggtgaa
      661 ggcctctacg gccatgtcga gctgttcggc ggtgtgcgcg gccgacatct gcgtccggat
      721 gcgtgccttg ccgtgcggta ccacggggaa cgagaagccc accacgtaca cgccttcctc
      781 cagtagccgg tcggccatgc gtccagccaa cgcggcgtcg tagaccatca ccgggattat
      841 cggatgtccg gcgcccgcca gatcgaagcc cgccgccgtc atggcgccgc ggaagtggtc
      901 gctgttggcc cgcagcctgg cgcgcagctc ggcgccgccg gacgacgagt cgtcctccag
      961 caggtcgagg acccgcagcg aggtggcggc gactaccggc gggatcgagt tcgagaacag
     1021 gtagggccgg ctgcgctggc gcaaccagtc gatgatctcg cgccggccgc tcgtgtagcc
     1081 gcccgacgcc ccgcctagcg ccttccccag cgtgcccgtg aggatgtcga ccctgtccat
     1141 gacgccgcga gcctcgtggc tgccgcgacc tccttcgccg acgaagccga cggcgtggct
     1201 gtcgtcgacc atgacgagcg cgtcgtagcg gtcggccagg tcacagatgg cgcgcaggtc
     1261 ggcgatgatc ccgtccatcg agaagacgcc gtcggtggct atgagtcgga accgcgcgcc
     1321 tgacgccgcc tgcagctgcc gctccaggtc ggccatgtcg ttgttggcgt agcggaacct
     1381 ggcggccttg ctgagcctga ccccgtcgat gatgctggcg tggttgaggg cgtcggagat
     1441 gatcgcgtct tcctcgccga gcagcgtctc gaacaggccg ccgttggcat cgaag
//
LOCUS       DAOQYE010000631         1495 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_106645_length_1495_cov_2.700000, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000631 DAOQYE010000000
VERSION     DAOQYE010000631.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1495
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106645_length_1495_cov_2.700000"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="PKN52_09640"
     CDS             <1..189
                     /locus_tag="PKN52_09640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00253.1"
                     /translation="VKANVFCTPGAGALDFDPVMAALQGVGYAGWYIVEAEQDPAVHD
                     PYRVSGAAREFLRDEYGL"
     gene            233..1264
                     /gene="iolG"
                     /locus_tag="PKN52_09645"
     CDS             233..1264
                     /gene="iolG"
                     /locus_tag="PKN52_09645"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050112 - inositol 2-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HNR00254.1"
                     /translation="MSVGKPVVEGSIGFAVIGAGRMGKLHARHLAGAVDGAHLVRVVD
                     ADGAAAARAAYGGAESGTDLAAALADPAVRAVVIASPTTLHAEHLMAAARAGKAIFCE
                     KPVAQDLESTAAALAVVAEAGVPFQIGFNRRYDPGYAEVARSVHAGELGRVEMFRSQS
                     SDPTPPPEEYVATSGGIYVDSVIHDIDMARFVAGEVTRVTALGRVLVAPYLEKHGDVD
                     TSILTLEFASGAIGVLQNTRRTLYGYDLRVEVHCERGKVVSEAERATNVWRYGEGGIR
                     GDYVYYFLERFRDAYLVEVQAFVSAVLAGERPTPGPEDAIGSLRVAVAARASLQRGEP
                     VAVSEFGPG"
BASE COUNT          206 a          514 c          557 g          218 t
ORIGIN      
        1 gtgaaggcca acgtcttctg cacgcccgga gcgggggcgc tcgacttcga ccccgtgatg
       61 gccgcgctgc agggcgtcgg ctacgcgggc tggtacatcg tggaagcgga gcaggacccg
      121 gcggtgcacg acccctaccg cgtgtcgggg gcggcccgcg agttcctccg cgacgagtac
      181 gggttgtgag cgtgccgctc gtctcgctgc tggtcgcgcc aaggagcttc ggatgagcgt
      241 gggcaaacca gtcgttgaag gtagcatcgg tttcgccgtc atcggggcgg gcaggatggg
      301 caagctgcac gcccggcatc tagcgggggc cgtcgacggg gcccatctcg tgcgcgtcgt
      361 cgacgcggac ggggccgcgg ccgcgcgcgc ggcttacggc ggtgcggagt cggggaccga
      421 cctcgccgct gccctcgccg acccggccgt gcgggccgtg gtcatcgcct ccccgacgac
      481 gttgcacgcg gagcacctca tggccgccgc ccgcgccggc aaggcgatct tctgcgagaa
      541 gcccgtcgcc caggacctgg agagcaccgc ggcggcgctc gcggtcgtgg ctgaagccgg
      601 ggtgccgttc cagatcggtt tcaaccggcg ctacgaccct ggctacgccg aggtggcgcg
      661 cagcgtgcac gccggcgaac tcggacgcgt cgagatgttc cgtagccagt cctctgaccc
      721 gacaccgccc cccgaggagt acgtggccac ctcgggcggc atctacgtcg actcggtcat
      781 ccacgacatc gacatggcgc gcttcgtggc cggcgaggtg acgcgcgtga cggccctcgg
      841 gcgcgtgctc gtcgccccct acctggagaa gcacggcgac gtcgacacga gcatcctcac
      901 cctcgagttc gcctccggcg ccatcggcgt gctgcagaac acgcgccgca cgctctacgg
      961 ctacgacctg cgagtcgagg tgcactgcga gcgtggcaag gtcgtctcgg aggccgagcg
     1021 ggccacgaac gtgtggcgct acggcgaggg cggcatccgg ggcgactacg tctactactt
     1081 cctggagcgc ttccgcgacg cctacctcgt cgaggtgcag gcgttcgtga gcgcagtgct
     1141 ggcgggcgag cggccgacgc ccggtccgga ggatgccatc gggtcgttgc gcgtggccgt
     1201 ggcggcgcga gccagcctcc agcgcggcga gccggtcgct gtgagcgagt tcggcccggg
     1261 ctgacgttga tgcgcgattc catccggcga cacccgccta ccgggcgcac cgcgccacgg
     1321 gcccatgttc tgggttctgc cctgccaagg tgggcatagc gaggagtgag cgacatggag
     1381 cacaaggtcc cttaccagcc gtccagcacg aggggcgtcg agatcgtgcc ggcgcgcgcc
     1441 ggctggaagt acctggggtt cgagacgcgc cgcgccgcgg cgggggagac gctcg
//
LOCUS       DAOQYE010000632         1494 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_106746_length_1494_cov_2.652536, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000632 DAOQYE010000000
VERSION     DAOQYE010000632.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1494
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106746_length_1494_cov_2.652536"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..874)
                     /locus_tag="PKN52_09650"
     CDS             complement(<1..874)
                     /locus_tag="PKN52_09650"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNR00255.1"
                     /translation="MPRGRRGRAAWVSLTGAVLILGLKFSAYLATGSVGFLSDAAESL
                     VNLVAAAVLLVALGVSVAPPDYRHPYGHAKAEYLSSVLEAALIIVAAGAIAWSAVGRL
                     LRPEALTNVAMGVGVSLLAALLNGLLAFYLFSVARRERSDALAANARHILTDVYTSLG
                     VVVGVLLVRFTGWQPLDPLVALVVAANIVVVGVRVMRQALSRLLDERLPEREEAKILA
                     VLEADPRVLGFHRLRTRRSGHASFAEVDVFVDPALTVKEAHDLVGRVEDAIHTELEDL
                     VTTIHVEPYVAGVRD"
     gene            complement(933..1379)
                     /locus_tag="PKN52_09655"
     CDS             complement(933..1379)
                     /locus_tag="PKN52_09655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00256.1"
                     /translation="MKGEKVLINGDLWMIVDVAPAMPLAEMVATILEDEGMITIVRGA
                     ENMADALSHLGLTSAQATYVLVPEADGARALAVIAETVTDFEGDELDELLERMEHGDI
                     PEELRDAWVDDGADGDLEGEAEARYEDAGWTSGATSRPDDAGEADT"
BASE COUNT          231 a          538 c          520 g          205 t
ORIGIN      
        1 ggtcgcgcac gcccgccacg tacggctcga cgtggatcgt ggtgacgagg tcctccagct
       61 cggtgtggat ggcgtcctcc acccggccga cgaggtcgtg cgcctccttg accgtcaacg
      121 ccgggtcgac gaagacgtcg acctcggcga agctcgcgtg ccctgagcgc cgcgtgcgca
      181 ggcggtggaa gccgagcacg cgcgggtcgg cctccagcac agccaggatc ttggcctctt
      241 cgcgctcggg taggcgctcg tccagaagcc gcgagagggc ctggcgcatg acgcggaccc
      301 ccacgaccac gatgttggcg gcgactacca aggccacgag cgggtcgagc ggttgccagc
      361 ccgtgaacct tacgagcagc acccctacca ccacgccgag cgaggtgtag acgtcggtga
      421 ggatgtggcg ggcgttggcc gccagggcgt ccgagcgctc cctgcgcgcc acggagaaga
      481 ggtagaaggc gaggaggccg ttgagcagcg ccgccagcag cgagacgccg acgcccatgg
      541 cgacgttggt cagcgcctcc ggtcgcagca gacgccccac cgcgctccac gcgatggccc
      601 ctgccgcgac gatgatgagg gcggcctcca acacgctcga gaggtactcg gccttggcat
      661 ggccgtacgg gtgccggtag tcgggcggcg ccacggagac gccgagagcc accaggagaa
      721 ccgccgccgc cacgaggttg acaagcgact cggcagcatc ggagaggaac ccgaccgagc
      781 cggtcgccag gtaggcggag aacttcaagc cgaggatgag caccgcgccg gtcaggctga
      841 cccacgccgc acggccgcga cgcccgcggg gcacctcgag cgctcctggc accgcgtgcg
      901 gtccggcgcc ggtgggagcg tccggactca tgtcaggtgt cggcttctcc ggcgtcgtcc
      961 ggccgtgacg tggcccccga ggtccaaccc gcgtcctcgt agcgcgcctc cgcctcacct
     1021 tcgaggtcgc cgtcggctcc atcgtcaacc cacgcgtccc tgagctcctc cgggatgtcg
     1081 ccgtgttcca tgcgctcaag gagctcgtcc agctcgtcgc cctcgaagtc cgtgaccgtc
     1141 tcggcgatca cggccagcgc gcgcgcgccg tcggcctcag ggaccaacac gtacgtcgcc
     1201 tgagcggacg tgagccccag gtgggagagc gcgtccgcca tgttctccgc accgcggacg
     1261 atggtgatca tgccctcgtc ctcgaggatg gtggcgacca tctccgccaa aggcatggca
     1321 ggagccacgt cgacgatcat ccagagatcg ccgttgatca gtaccttctc gcccttcata
     1381 ccgcctcccc gtgccccgct cgtgcgaggt atgccgtggc gaacccggcg taggcgcggc
     1441 gggccgcgtg caggtcctcg ccctgcgcgt agagcgcggc gcgcgggagc ggca
//
LOCUS       DAOQYE010000633         1494 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_106748_length_1494_cov_2.620570, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000633 DAOQYE010000000
VERSION     DAOQYE010000633.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1494
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106748_length_1494_cov_2.620570"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1494)
                     /locus_tag="PKN52_09660"
     CDS             complement(<1..>1494)
                     /locus_tag="PKN52_09660"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNR00257.1"
                     /translation="RTWTFHLRSGVKWSDGAPFNASALTFWYTRVILNKDLTPSVPAW
                     FANRDGTAAKLEAVDDYTVRITFDFPNTLFLTELTFRDGGDRTLAAFLPGHYLQQFHP
                     DFQSAEELDAKVKAAGLSNWTDLFMTKAMPTENPERPTMAAWVPFNSTVSDQVFTLRR
                     NPYYIGVDPAGNQLPYIDEVQFRYFADVQALNFAAVAGELDFQARHIQMPNYPVLVEN
                     SDRSNYRVMLWPSFGGSDAAVWFNQEYEIDPELGSILANHDFRVALSYATNRDEIRES
                     AFLGLGEIRQNVPAPWHPYYPGDEYAHKYTEYNPDLANQMLDAIGLTKDANGNRLMPS
                     GKPLRVELSVVPAFGPWPDVAQLVAADWAAVGVPTDVQVRERNAHFTMRDANELQVEI
                     WNEDTTAFPFTGNPKIDPRSNPATIFAVESRRWYESGGTAGREPAPGIARIVDIIEEA
                     KTVGVEQQIELAKELFRHAVDELYGFGLVGLTPMVQGVVVVNDDLMNV"
BASE COUNT          220 a          438 c          572 g          264 t
ORIGIN      
        1 gacgttcatg aggtcgtcgt tgacgacgac gacgccctgg accatcggcg tgaggccgac
       61 gaggccgaag ccgtacagct cgtcgacggc gtggcggaag agctccttcg ccagctcgat
      121 ctgctgctcg acaccgacgg tcttggcctc ttcgatgatg tcgacgatgc gggcgatgcc
      181 cggagcgggc tccctgccgg cggtgccgcc ggactcgtac cagcggcgcg actcgacggc
      241 gaagatggtc gccgggttgc tacgcgggtc gatcttgggg ttgcccgtga aggggaacgc
      301 cgtggtgtcc tcgttccaga tctcgacctg gagctcgttg gcgtcgcgca tcgtgaagtg
      361 ggcgttgcgc tcgcgcacct ggacatcggt cggcacgcct accgcggccc agtcggcggc
      421 cacgagctga gccacgtcgg gccacgggcc gaaggccggg acgacggaga gctccacgcg
      481 gagcggcttg ccgctcggca tgaggcggtt gccgttggcg tccttcgtga ggccgatggc
      541 gtcgagcatc tggttggcga ggtcggggtt gtactccgtg tacttgtggg cgtactcgtc
      601 gcccgggtag tacgggtgcc acggcgccgg gacgttctgg cggatctcgc cgaggccgag
      661 gaacgcggac tcgcggatct cgtcgcggtt ggtggcgtac gagagggcca cgcggaagtc
      721 gtggttggcg aggatgctcc cgagctctgg gtcgatctcg tactcctggt tgaaccagac
      781 ggcggcgtcg gagccgccga agctcggcca cagcatgacg cggtagttcg agcggtcgga
      841 gttctcgacc agcacggggt agttgggcat ctggatgtgg cgggcctgga agtcgagctc
      901 accggccacg gccgcgaagt tcagggcctg cacgtcggcg aagtagcgga actgcacctc
      961 gtcgatgtac gggagctggt taccggccgg gtcgacgccg atgtagtacg ggttgcggcg
     1021 cagcgtgaac acctggtcgg agaccgtcga gttgaagggc acccaggcgg ccatggtcgg
     1081 gcgctcgggg ttctcggtcg gcatggcctt cgtcatgaac aggtcggtcc agttggagag
     1141 accggcggcc ttgaccttcg cgtcgagctc ctcggcgctc tggaagtcgg ggtggaactg
     1201 ctgcaggtag tggccgggga ggaaggcggc cagggtcctg tcgccgccgt cgcggaacgt
     1261 gagctccgtc aggaacagcg tgttcgggaa gtcgaacgtg atgcggaccg tgtagtcgtc
     1321 gacggcctcg agcttggcag ccgtgccgtc gcggttcgcg aaccaggccg gcacgctcgg
     1381 ggtcaggtcc ttgttcagga tgacgcgggt gtaccagaac gtgagggcgc tggcgttgaa
     1441 cggcgcgccg tcggaccact tgactcctga gcgcagatgg aacgtccagg tcct
//
LOCUS       DAOQYE010000634         1494 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_106762_length_1494_cov_2.347464, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000634 DAOQYE010000000
VERSION     DAOQYE010000634.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1494
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106762_length_1494_cov_2.347464"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="PKN52_09665"
     CDS             <1..281
                     /locus_tag="PKN52_09665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00258.1"
                     /translation="IFALVDDRDRAYFGRDGDDRDQIGYIDEQTRAVFGRSYAAEPEV
                     LVKQLAEDEAIAEADTLLLTVPNQLGVEYNAHVIEAILKHVAPELGWR"
     gene            272..805
                     /locus_tag="PKN52_09670"
     CDS             272..805
                     /locus_tag="PKN52_09670"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:TIGR00589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HNR00259.1"
                     /translation="MAVTLTSSRFTTPLGSMLGVATEEAVVLLEFEERRDLARELEDL
                     ARDAAIVDGTNAPLERLKRELDGYFAGELAEFKTPLELRGTPFQMRAWQELLKIPAGA
                     TITYLQLAQAVGNPNSFRAVAQANGANRLAVVVPCHRVVNTNGGLGGYAGGLERKRWL
                     LEHERTVFGGRVEGRLF"
BASE COUNT          238 a          455 c          519 g          282 t
ORIGIN      
        1 gcatcttcgc cctcgtggac gaccgtgacc gcgcctactt cggccgcgac ggcgacgacc
       61 gcgaccagat cggctacatc gacgagcaga cacgcgccgt cttcggccgc agctacgccg
      121 ccgagccgga agtactcgtc aagcagttgg cggaggacga ggcgatcgcg gaggcagaca
      181 cgctgctcct gaccgtgccc aaccagctgg gagtggagta caacgcgcac gtcatcgagg
      241 cgatcctcaa gcacgtggcg ccggagttgg ggtggcggtg acgctgacct cctcccgctt
      301 cacgacgccg ctaggctcca tgctcggcgt ggccaccgag gaagccgtcg tgctgctcga
      361 gttcgaggag cgccgcgacc tggcgcggga actcgaggat ctcgcgcgag acgccgccat
      421 cgtcgacggg acgaacgcgc cgcttgagcg cctgaagcgt gagttggacg gttacttcgc
      481 aggcgagctc gccgagttca agacaccgct ggagctgcgc gggacgccgt tccagatgcg
      541 cgcctggcag gagctcctca agatccccgc cggggcgacc atcacgtacc tgcaactggc
      601 gcaggccgtg ggcaacccga actcgttcag ggcagtggcg caggccaacg gcgccaaccg
      661 ccttgccgtg gtcgtgccgt gccaccgcgt cgtcaacacg aacggcggtc tcggcgggta
      721 cgccgggggg ctcgagagga agcggtggct gctggagcac gagcgcacgg tgttcggcgg
      781 acgggtcgag ggcaggctgt tctagggcgt gttcgcacta tgttgtgtgg tgcttctgat
      841 gggtaggcag gggtggtgag ggcggggtaa tgggggccgg ttggtggcgt cttcttgagc
      901 gtcgcgttcg tcactgacta actacgaagt gtgagcacgc cccaactacg gagtgcgggt
      961 ttctccccca atgaggtacc tgagtgcggt ctaggcgctc gggtcgagcg ttgcgcttct
     1021 cgctctcgag gtccgggggt cgggggttgg ggttcttctt gagaatcggg tactcgtgca
     1081 accatctgat tgcgcaactg cctcgttgca tatcatgtcg tttttcaaac tagacctccc
     1141 ttggtcgccg acctcccctg gtcgccgact tcccctggtc gccgtgctgg cttgggggcc
     1201 atgttcgcca tgctggcttg gacagcagcg tcttggaagg tagccccgtg ttgcatccca
     1261 tgcgctgtac gcttagaacc caagcagggt gcgcaaacgg ttcttgagtc gtcgaggagc
     1321 gcgtgcccat ggagcgtctt gtcgagttcg atcatcgcgt gggaggagat tcgtggttcg
     1381 tccttgatcg tgtctcagtg atgcgtgact cctgacgttg tgccgtcaac agtgaccaga
     1441 ccctagtcgc gcacgccgag cgcaacagcc gagtcgcata cgccaggcgc ggcg
//
LOCUS       DAOQYE010000635         1489 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_107385_length_1489_cov_2.266388, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000635 DAOQYE010000000
VERSION     DAOQYE010000635.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1489
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107385_length_1489_cov_2.266388"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /gene="rpsD"
                     /locus_tag="PKN52_09675"
     CDS             <1..364
                     /gene="rpsD"
                     /locus_tag="PKN52_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HNR00260.1"
                     /translation="GTVFLQLLESRLDNVVYRLGIAYTRRQARQFVAHGHILVNGKRV
                     DVPSYSVRPGDTIEVSANAKKNSVIQANIEDRKRGKLNPWLEFDSETMKGRFLHRPSR
                     EDIMVPVNELQVIEYYSR"
     gene            406..1344
                     /locus_tag="PKN52_09680"
     CDS             406..1344
                     /locus_tag="PKN52_09680"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HNR00261.1"
                     /translation="MHIIPEFKAKVSGSYGEFVVEPLPRGYGVTLGNPLRRILLSSVP
                     GTAVTSVYIEDVLHEFSTIDGVKEDVMQIILNLKDLVVKMHDEDPVTLTLRADQPGEV
                     TAAMFDVPSTAEVVNPGLHIATLSKGGRLVMEVRVERGVGYVPAEIHGTKDRINSIPV
                     DAIYTPVRRVAYRVEDTRVGQKTDLDRLTLRVWTDGSIDPRDALDIAVDTLRGQLNVF
                     SGDGGLVEEPTPVLVIPNGKVEETSAPVEEVTLSLESLDLSSRVLHSLQEEGIDSVNA
                     LLALSERDLKKVGGVGEKSLEEIKERLAARGYNLKE"
     gene            1362..>1489
                     /locus_tag="PKN52_09685"
     CDS             1362..>1489
                     /locus_tag="PKN52_09685"
                     /inference="COORDINATES: protein motif:HMM:NF013369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L17 family ribosomal protein"
                     /protein_id="HNR00262.1"
                     /translation="MRGRKLSRSSSDRSALARSQATALLRHGRIKTTLPKAKSLQP"
BASE COUNT          283 a          529 c          466 g          211 t
ORIGIN      
        1 gggcaccgtc ttcctccagc tcctcgagtc gcgcctcgac aacgtggtct accgcctcgg
       61 catcgcctac acgcggcgcc aggcgcgcca gttcgtcgct cacggtcaca tcctcgtgaa
      121 cggcaagcgc gtcgacgtcc cgtcgtacag cgtgcgcccc ggcgacacca tcgaggtgtc
      181 cgccaacgcc aagaagaaca gcgtcatcca ggccaacatc gaggaccgca agcggggcaa
      241 gctgaacccc tggctggagt tcgactcgga gaccatgaag ggccgcttcc tgcaccgccc
      301 ttcccgcgag gacatcatgg tcccggtcaa cgagctccag gtcatcgagt actactcgcg
      361 ctgagcggcg agcgcaccaa ctaagtcgct accgaggagg taccggtgca catcatccca
      421 gagttcaaag ccaaggtctc gggcagctac ggcgagttcg tcgtcgagcc gctaccgcgc
      481 ggctacggcg tcacgctcgg caacccgctc aggcgcatcc tgctgtcctc cgtgcccggc
      541 acggcggtca cgagcgtgta catcgaggac gtcctgcacg agttctcgac catcgacggg
      601 gtcaaggagg atgtcatgca gatcatcctc aacctcaagg acctggtcgt gaagatgcac
      661 gacgaggacc ccgtcacgct caccctgcgc gccgatcagc ccggcgaggt aacggccgcc
      721 atgttcgacg tcccgtcgac ggcggaggtc gtgaaccccg ggctgcacat cgccaccctc
      781 tccaagggcg gcaggctcgt catggaagtg cgcgtggaac gcggcgtcgg ctacgtcccc
      841 gccgagatcc acggcacgaa ggaccgcatc aactccatcc cagtcgacgc catctacacg
      901 ccggtgaggc gcgtggccta ccgcgtcgag gacacccgcg tcgggcagaa gaccgacctc
      961 gaccgcctca cgttgcgcgt ttggacggac ggcagcatcg acccgcgcga cgcgctcgac
     1021 atcgcggtcg acacgctgcg cggccagctc aacgtcttca gcggcgacgg cggcctcgtt
     1081 gaggagccca cgccggtcct cgtcatcccc aacggcaagg tcgaggagac gagcgcgccc
     1141 gtcgaagagg tcacgctcag cctggaaagc ctcgacctgt ccagccgcgt gctgcactcg
     1201 ctgcaagaag aggggatcga ctccgtcaac gccctgctcg cgctctccga gcgcgacctc
     1261 aagaaggtcg gcggggtcgg cgagaagtcc ctcgaagaga tcaaggagcg cctcgcggcg
     1321 cgcggataca acctaaagga gtgacgtaga tgcgccatat gatgcgcggc cgcaagttga
     1381 gccgctccag cagcgaccgt tccgcgctgg cccgcagcca ggcgacggcg ctgctgcggc
     1441 acggccggat caagaccacg ctgccgaagg cgaagagcct gcagccgtt
//
LOCUS       DAOQYE010000636         1488 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_107481_length_1488_cov_2.295883, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000636 DAOQYE010000000
VERSION     DAOQYE010000636.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1488
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107481_length_1488_cov_2.295883"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="PKN52_09690"
     CDS             complement(<1..427)
                     /locus_tag="PKN52_09690"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNR00263.1"
                     /translation="MYSGTMPEYFVPNAKRRRGRPKGGATDARSRIVAAAAEEFGEFG
                     YDAATIRGIAARAGVDSALVHHYFGTKADLFAETIEFPLRPDLAVPGILAGPREEIGE
                     GIVRFVLGRLEDVDARKRAVALLRSSLGNRLATPLVAGFL"
     gene            468..1379
                     /locus_tag="PKN52_09695"
     CDS             468..1379
                     /locus_tag="PKN52_09695"
                     /inference="COORDINATES: protein motif:HMM:NF012703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HNR00264.1"
                     /translation="MPYTVIVSQGRVADRTPGAIRGAALLGAALAERLGLSVTVCGTP
                     SLPAADAWPEALAGAEPTLVALADAVKSALDGGSTPLLALNKCPASLATLPAAAAHWP
                     GPNRTGAPEPGSTAPDLSGPGLKVLWIDAHGDFNTPTTTATGYLGGMVLAAACGLWDS
                     GHGAGVAGENVVILGGHDLDEAEAALLAAAGATVLLPTLGSDEAVARAVNAALGDGPI
                     WVHLDCDALEPGHVPADYVVPGGFSPATLKTVLAALPVERVVGIEIAEFEASPDAAES
                     VEEPGPTTRQGLETLLDIVAPLLANLR"
BASE COUNT          216 a          537 c          539 g          196 t
ORIGIN      
        1 gcaggaagcc ggccaccagc ggcgtggcga gccggttgcc gaggctcgac cgcagtagcg
       61 ccaccgcgcg cttgcgcgcg tcgacgtcct ccagccggcc gagcacgaag cgcacgatgc
      121 cctcgccgat ctcctcgcgg ggaccggcga ggatgccggg aacggcaagg tccggccgca
      181 gcgggaactc gatcgtctcg gcgaagaggt cggccttggt gccgaagtag tggtgaacga
      241 gcgccgagtc gacgccggcg cgtgccgcga tgccgcggat cgtcgccgcg tcgtacccga
      301 actcgccgaa ctcctcggcc gcggcggcga cgatgcggct gcgcgcgtcc gtcgccccgc
      361 ccttcggacg accccggcgg cgcttcgcgt tcggcacgaa gtactcgggc atggttccag
      421 agtacatgcg tggaggctcg acgcccggcc acgctagtct ggagcccatg ccctacacgg
      481 tgatcgtgtc gcaaggccgc gtagccgaca ggaccccggg ggccatccgg ggtgccgccc
      541 tcctaggcgc ggcattggcc gagaggctag gtctgagcgt cacggtctgc ggcacgccat
      601 cactgcccgc cgccgacgcc tggccggaag cgctggcagg cgccgagccc acgctggtcg
      661 cgctggccga cgcggtcaag tcggcgctcg acggcggctc aacgccactg ctcgccctca
      721 acaagtgccc cgccagcctc gccacgctgc cggcggcggc cgctcactgg ccgggtccga
      781 accggacggg cgctcccgaa cccggctcca ccgcacccga cctgagcggt cccggtctca
      841 aggtcctgtg gatcgacgct cacggcgact tcaacacgcc gaccaccacg gccaccggct
      901 acctcggcgg catggtgctg gcagccgctt gcggcttgtg ggacagcggg catggcgccg
      961 gtgtcgcggg agagaacgtg gtgatcctcg gcggacacga cctggacgag gcggaggccg
     1021 cgctgctcgc ggcggcggga gcgaccgtcc tactccctac gctcggctcc gacgaagccg
     1081 tcgcccgggc ggtcaacgct gcactaggcg acggaccaat atgggtccac ctcgactgcg
     1141 acgcgctcga gccgggccat gtgcccgcgg actacgtggt gcccgggggc ttctcgccgg
     1201 ccacactcaa gacggtgctg gccgcgctgc ccgtggagcg cgtcgtgggc atcgagatcg
     1261 cggagttcga ggcctcgccc gatgctgcgg agagcgtaga ggagcctggc cccacaacgc
     1321 ggcaaggtct cgagaccctg ctggatatcg tggctcccct gttggcgaac ctgcgctgac
     1381 ggaagctaac tgtcgatgaa cgagggagcg cgccgaacgc cggcctgacg gagccgtcgg
     1441 caggcgacag gtcaggcccg ggggttggtc gcccgggtgc cggccgcg
//
LOCUS       DAOQYE010000637         1487 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_107585_length_1487_cov_2.510475, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000637 DAOQYE010000000
VERSION     DAOQYE010000637.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1487
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107585_length_1487_cov_2.510475"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..658)
                     /locus_tag="PKN52_09700"
     CDS             complement(<1..658)
                     /locus_tag="PKN52_09700"
                     /inference="COORDINATES: protein motif:HMM:NF016817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HNR00265.1"
                     /translation="MSAFRHLGLEVAPGARGMTRLPVSDVAGGYSLGVPLHVLNGAHP
                     GPTLLVVGVVHGEEIFAIDAIRTALASVDLARLHGTVIAVPVANPPALAANTRNNPLD
                     MLDLNRQFPGSEDGWLSQRIAAAISPLIDRSDCLLHIDGGAIERVIHYVFVKSDGAGN
                     GVTERLSRAFGLKLLYRGSHSGGSITSYAAGRGIPAVLAEIGGSTLYTDPRYLQRAST
                     G"
     gene            complement(655..>1487)
                     /locus_tag="PKN52_09705"
     CDS             complement(655..>1487)
                     /locus_tag="PKN52_09705"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HNR00266.1"
                     /translation="DYLCLDEVARGRVRRARSVADVWNADADGLLSLVYGLQAADPFE
                     GDTYLVRIFQELGVRVANLVYNQRNRLGDGCMERVDQGLTAFGRSVVLEMNRVGMTVD
                     LSHTGERTSLDAAGCSRAPVVFSHSCAKALTDHPRNLTDEQIRAAAATGGLVGLCPHS
                     QFCEKERGVRPTLNDFLDHLVYVAELVGVEHVGIGTDLFGGKTLGESVFRFQFGSLVP
                     GAWGGYGIDEKYVAGFDTVFEWPNVTRGLVARGFGDEEIIAILGGNWLRVFEQTWSGR
                     "
BASE COUNT          245 a          503 c          503 g          236 t
ORIGIN      
        1 cgccggtaga agcgcgttgc aggtacctcg ggtcggtata gagggtgctg ccgccgatct
       61 cggccagcac cgccgggatc ccgcgccccg ccgcgtaact ggttatcgag ccaccagagt
      121 gtgagccgcg atacaggagc ttcagtccga acgccctcga gaggcgttcc gtcacgccgt
      181 tccccgcacc gtcggacttc acgaagacgt aatggatgac gcgctctatg gctccgccgt
      241 cgatatggag gaggcagtcg ctgcggtcta tcaaagggct gatggcggcg gcgatgcgtt
      301 ggctcaacca cccatcctcg ctgcccggga actggcggtt gaggtcgagc atgtcgaggg
      361 ggttgttacg ggtgttggcc gccaacgcgg gcgggttcgc caccggaacg gctatgaccg
      421 tcccgtgcag acgggccagg tcgacgctcg ccaacgccgt gcggatggcg tcgatcgcga
      481 agatctcctc gccgtggacg acgccgacga ccagcagcgt cggccccgga tgcgcaccgt
      541 tgagcacgtg cagcggaacg ccgagggagt agccgcccgc cacgtcggac acgggcaggc
      601 gggtcatgcc gcgcgcgccc ggtgcgacct cgagccccag atgacggaag gcgctcatct
      661 gccgctccag gtctgttcga agaccctcaa ccagttgccg cccaggatgg cgatgatctc
      721 ttcatcgccg aacccgcgcg ccaccaagcc gcgcgtgacg ttgggccact cgaacaccgt
      781 gtcgaagccg gcgacgtact tctcgtcgat gccgtacccg ccccaggcgc ccggcacgag
      841 gctcccgaac tggaagcgga agacggactc gccaagggtc ttgccgccga acaggtcggt
      901 gccgatgccg acgtgctcta cgccgactag ttcggccacg tagacgaggt ggtcgaggaa
      961 gtcgttcagg gtggggcgca cgccgcgctc cttctcgcag aactgcgagt gtgggcacag
     1021 gccgacgagc ccacccgtgg ccgctgcggc ccggatctgc tcgtccgtca ggttgcgtgg
     1081 gtggtcggtt aaggccttgg cgcaggagtg cgagaacacg acgggcgcgc gcgagcatcc
     1141 ggccgcgtca agagacgtcc gctcgcccgt atgggagagg tcgaccgtca tacctacccg
     1201 gttcatctcg aggaccacgc tgcgcccgaa cgctgtgagt ccctggtcga cgcgttccat
     1261 gcacccgtcg cccaagcggt tgcgctggtt gtacacgagg ttggctaccc gcacacccag
     1321 ttcttggaag atcctcacga ggtacgtgtc gccctcgaag gggtcggcgg cctgcagccc
     1381 gtagacgagg ctaagcagcc cgtccgcgtc ggcgttccat acgtccgcca cgctccgggc
     1441 gcggcgcacc ctgcccctgg cgacctcgtc gagacacagg tagtcgt
//
LOCUS       DAOQYE010000638         1487 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_107613_length_1487_cov_2.024441, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000638 DAOQYE010000000
VERSION     DAOQYE010000638.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1487
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107613_length_1487_cov_2.024441"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(113..>1487)
                     /locus_tag="PKN52_09710"
     CDS             complement(113..>1487)
                     /locus_tag="PKN52_09710"
                     /inference="COORDINATES: protein motif:HMM:NF014757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HNR00267.1"
                     /translation="LQRYRAFVPDERIHLIFLEDYAADARATLDGVCRFLGVDPLSEA
                     LPVEAVAVLGAGLMGSGIAEVSVAGGMRVLLKDRDLDLALKGKVNVYRGLSSRVGKGR
                     TAFERDQLVERVRPVDDYAKVAGAQLTIEAVLEEPALKRTVLADVERATAGERHVFAS
                     NTSAIRIAEIAEGALRPEAVIGMHYFSPVPKMPLLEIVVTDRTADWATATAFEVGARQ
                     GKTTIVVNDGPGFYTTRVLSFYVAEAMRVLEEGADPRALEAAMVSYGFPLGPLALLDD
                     VGIDVGAKIEKVLEPLMTARGLVRSRASAELVAAGYLGRKAGKGFYRYEDGRRTKELD
                     TEALRIAGFRGGDRRGAEAATRSGSDGTSAELTQRLVLTFVREAVLCLEEGVLRSARD
                     GDVGAVFGLGFPPFRGGPFFLIDREGAASVVARLSALAQRHGERFAPPRSLVELAESG
                     GRFHGGA"
BASE COUNT          228 a          575 c          484 g          200 t
ORIGIN      
        1 gcgtccaccc atagcaaccc ggtcttgccc tgcgccttct tcgcgcgcgc cagggcggcg
       61 gacaggctcc ccgcggacgg gtgcgcgagg accagcgtaa ctaccggggc gctcatgcgc
      121 ccccatggaa acgtccgccg gactccgcca gctcgacgag cgatcgcggg ggcgcgaacc
      181 gctcgccgtg cctctgggcg agcgcgctca agcgcgccac cacgctcgcc gcaccctcgc
      241 ggtcgatcag gaagaacgga ccgccgcgga acggcgggaa gccaaggccg aacacggcgc
      301 cgacatcgcc gtcgcgcgcc gagcgcagta cgccctcctc caggcagagc accgcctcgc
      361 gcacgaacgt caggaccagg cgttgggtca gctccgcgct cgtgccgtca gacccgcttc
      421 gcgtcgcggc ctccgctccc cgcctatcac ccccgcggaa ccccgcgatc ctgagcgcct
      481 cggtgtccag ctccttcgtg cgccgcccgt cctcgtagcg gtagaacccc ttgcccgcct
      541 tgcgccccag gtaaccggcg gcgacgagct ccgcgctggc gcggctccgc acgaggccgc
      601 gcgcggtcat gagcggctcc aggaccttct cgatcttggc cccaacgtct atcccgacgt
      661 cgtcgaggag agccagcggc ccgagcggga agccgtagct caccatcgcc gcctcgaggg
      721 cgcgcgggtc cgcaccttcc tccagcacac gcatggcctc ggcgacgtag aacgacagga
      781 cgcgcgtcgt gtagaagccg gggccatcgt tgacgacgat ggtcgtcttg ccctggcgcg
      841 cgccgacctc gaaggccgtg gcggtggccc agtcggccgt gcggtcggtg acgacgatct
      901 cgagcagcgg catcttcggc acgggcgaga agtagtgcat gccgatcacc gcctctggcc
      961 gcagcgcgcc ctcggcgatc tcggcgatgc ggatggcgga cgtgttggag gcgaacacgt
     1021 gccgctcccc ggcggtcgcg cgctcgacgt cggccagcac cgtccgtttc aaggccggct
     1081 cctcgagcac ggcctcgatg gtcagttgcg cccccgcgac cttcgcgtag tcgtcgaccg
     1141 gcctcacccg ctccacgagc tggtcgcgct cgaacgcggt ccgccccttc ccgacgcgcg
     1201 acgacaggcc gcgatagacg ttcaccttcc ccttgagggc gagatcgagg tcgcggtcct
     1261 tcaggagcac ccgcatgccc cccgcgacag atacctccgc gatgccggag cccatgaggc
     1321 cagcaccgag cacggccaca gcctcgacgg ggagcgcctc gctcagcgga tcaacgccca
     1381 ggaaccggca caccccgtcc agcgtcgcgc gcgcgtcggc ggcatagtct tccagaaaga
     1441 tcaggtgaat gcgttcatcc ggcacgaacg cgcgatagcg ctgcaac
//
LOCUS       DAOQYE010000639         1486 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_107650_length_1486_cov_3.991614, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000639 DAOQYE010000000
VERSION     DAOQYE010000639.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1486
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107650_length_1486_cov_3.991614"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(202..1107)
                     /locus_tag="PKN52_09715"
     CDS             complement(202..1107)
                     /locus_tag="PKN52_09715"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNR00268.1"
                     /translation="MDTAAAPADSRSDGNEGSGGSGRSNASAAWRRFRRSSSGVFGLT
                     LVVLLALVALLAPVIAPYDPHSTSRAAFAPPLSAGHVLGTDNLGRDVISQVIWGSRVS
                     LMVGLAAAATATLIGVIVGAFAGYLGGRFDELLMRVTEFFQVIPRFVLALIVVAFFGS
                     GLVNLILVIGVLSWPQTARLVRSQYLSHKTLPYVDAGRAIGMRSFRIMFMQILPNAMG
                     PVLVVASLDVAQAILLEASLGFFGLGDPNLTSWGGMLNTAQTFIRRAWWMSVFPGIGI
                     TLAVLGFNLFGDGLTEAYDPRMAPR"
     gene            complement(1108..>1486)
                     /locus_tag="PKN52_09720"
     CDS             complement(1108..>1486)
                     /locus_tag="PKN52_09720"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNR00269.1"
                     /translation="FDDEPNVDWNATRASLLEVMSAQYIVAARGRGLSENTILWRHAL
                     RAAALPVVTVIGYNFGFIVAGSALVETVFGWPGIGRLMYDSIYTRDYPVLLGILLFVS
                     ITVIVVNLITDAFYAVLDPRVRY"
BASE COUNT          263 a          471 c          543 g          209 t
ORIGIN      
        1 cggccgtgcg gcccgtgaag agcgtgtact cgcggttgcg cgcgatcagc ttcggctcga
       61 cgaaggactc ggcgatgcgc tcggcgcggc ccgggtcgcc ggggatgagg acgtaagggg
      121 cgacgtcgcc cgtcttggcg cgcaggtggc gttggatgtc tgacatgtcg ctcttctcct
      181 aaaggtgcgg gcgccgggtc ttcaccgcgg cgccatgcgc gggtcgtacg cttccgtgag
      241 tccgtcgccg aacaggttga agccgagcac ggccagcgtg atgccgatgc cggggaacac
      301 gctcatccac caggcgcggc ggatgaaggt ctgggcggtg ttgagcatgc cgccccagct
      361 cgtcaggttc gggtcgccga ggccgaagaa gccgaggctg gcctcgagga ggatcgcctg
      421 cgccacgtca agcgaggcga cgacgagcac gggccccatg gcgttaggca ggatctgcat
      481 gaacatgatg cggaagctgc gcatgccgat ggcgcgcccg gcgtcgacgt acggcagcgt
      541 cttgtggcta aggtactggc tgcgcaccag ccgcgccgtc tgcggccagc tcagcacccc
      601 gatgacgagg atgaggttca cgagcccgga accgaagaac gccaccacga tgagggccag
      661 cacgaagcgc gggatcacct ggaagaactc cgtgacccgc atgaggagct cgtcgaaccg
      721 gccgccgagg tagcccgcga aggcgccgac gatgacgccg atcaacgtcg cggtggcggc
      781 cgccgcgagc ccgaccatca gcgagacccg ggagccccag atgacttggc tgatcacgtc
      841 acgcccgagg ttgtccgtgc cgaggacatg gccggcggag agtggcggcg cgaaggctgc
      901 gcgcgacgtg ctatgcgggt cgtacggtgc gatgacgggc gccaggaggg cgacgagcgc
      961 gagcagcacg acgagcgtca ggccgaagac gccggaactg ctccgcctga agcggcgcca
     1021 cgcggcgctg gcgttagagc gcccgctgcc gccggagcct tcgttcccgt cggagcggct
     1081 gtccgctgga gcggctgccg tgtccactca gtacctcact cgcgggtcga gaacggcgta
     1141 gaacgcgtcg gtgatgagat ttaccacgat gacagtgatg gatacgaaca gcaagatacc
     1201 gagcaacacg gggtagtcgc gcgtgtagat ggagtcgtac atgagccggc ctatgcccgg
     1261 ccaaccgaac accgtctcca ccagggcgga gcccgccacg atgaagccga agttgtagcc
     1321 gatgaccgtc acgacaggca gcgcggcggc cctgagggcg tgccgccaga ggatggtgtt
     1381 ctccgacagc ccgcgccccc gcgcggccac gatgtactgg gcgctcatca cctcgaggag
     1441 cgacgcgcgg gtggcgttcc agtcgacgtt cggttcgtcg tcgaag
//
LOCUS       DAOQYE010000640         1486 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_107674_length_1486_cov_3.033543, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000640 DAOQYE010000000
VERSION     DAOQYE010000640.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1486
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107674_length_1486_cov_3.033543"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..711
                     /locus_tag="PKN52_09725"
     CDS             <1..711
                     /locus_tag="PKN52_09725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00270.1"
                     /translation="PVGVRAWLQTPAAAWVFAAFSLLVLFALTRRGSTLRRWLAAGMG
                     AIREHRRLVVGTLVALYALFGLGAYVGSTLPQECEQAVLDVLNADIGSVGATQAYGSG
                     NVVRAAATTYFQNFAVVSTSMLLPLAALFALPAYLFAGLSFFAQGIPFGLLASGGVGY
                     TVLLVLLLVIELTSYFLVVAGGGMFLATLFRGRKTTAPGGPFTRGFRKLLLMLPITGL
                     LLLVGAWYEALIIILGLA"
     gene            complement(813..>1486)
                     /locus_tag="PKN52_09730"
     CDS             complement(813..>1486)
                     /locus_tag="PKN52_09730"
                     /inference="COORDINATES: protein motif:HMM:NF013811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00271.1"
                     /translation="LTGANAAAVMGRHYGPWPGPTLQRLMEERGTLFTEASEFGGAVL
                     ANAYPPAYFAAMGGRRHRPNAPAFAALAAGLELRDLDAYRAGGAAAADLTGERFAAMD
                     PSLSAHGAAGTAAALLGLASAAAFTFFDVWPTDALGHAQDFTGATRLLGLLDDVLSRL
                     GGRGVTVVVTSDHGNLEDVGSGQHTDAEVPLLVLGPHAEEFAGATSLVDVAPAIRGAW
                     RRTGR"
BASE COUNT          183 a          550 c          518 g          235 t
ORIGIN      
        1 ccagtcgggg ttcgcgcctg gctccagacg cccgcggcgg cgtgggtgtt cgctgccttc
       61 tcgctcctcg tgctgttcgc cctgacgcgt cgcggctcga ccctgcgcag atggctcgcc
      121 gccggcatgg gcgcgatccg cgagcaccgg cgcctcgtcg tcggcaccct cgtcgcgctc
      181 tacgctctct tcggtctcgg agcctacgtc ggctcgacgc tccctcagga gtgcgagcag
      241 gcggtgctag acgtgcttaa cgccgacatc ggcagcgtcg gggccacgca ggcttacggg
      301 agcggcaacg tcgtgcgcgc cgccgccacc acctacttcc agaacttcgc cgtcgtctcg
      361 acatccatgc tgctgccgct ggccgcgctc ttcgccctgc ccgcctacct cttcgccggc
      421 ctgtcgttct tcgcgcaagg catcccgttc ggcctactgg cgagcggcgg cgtaggttac
      481 accgtcctgc tcgtcctgct gctcgtgatc gagctcacgt cgtacttcct ggtcgtcgcg
      541 ggcggcggca tgttcctagc caccctgttc agggggcgca agacgacggc gccgggcggg
      601 ccgttcacgc ggggcttccg caagctgctc ctgatgctgc ccataacggg cctcctgctg
      661 ctggtcggcg cctggtacga agccctgatc atcatcctcg ggttggcgta gcccggcgca
      721 gccgtgcggc ggcgcgtaga ccgtcggaca gcgccgtact cgacgccgta ttcggcgcac
      781 cgtgctcggc gccccgtctt cagcgcacgt cttcagcgcc ccgtgcggcg ccacgctccg
      841 cggatggccg gggccacgtc gacgagcgag gtcgctccag cgaactcctc cgcgtgcggg
      901 cccaagacca gcagcggcac ctcagcgtcg gtgtgttgcc cggagccgac gtcctcgagg
      961 ttgccgtggt cggaggtgac caccaccgtc acgccacgtc cgccgagccg cgacagcacg
     1021 tcgtcgagca gcccgagcag gcgagtggcg ccggtgaagt cctgagcgtg gcccagggcg
     1081 tcggtcggcc aaacgtcgaa gaacgtgaaa gcggccgcgc tggccagccc gagcagcgcc
     1141 gcggccgtcc ccgcggcgcc gtgggcggag aggctgggat ccatcgctgc gaaccgctcg
     1201 ccggtcagat cggcggcggc cgctccgccc gcgcggtagg cgtcgaggtc gcggagctcg
     1261 agaccggcgg ccagcgcggc gaacgcgggg gcgttcgggc ggtggcgtct gccgcccatg
     1321 gcggcgaagt aggccggcgg gtaggcgttg gccagcacgg ccccgccgaa ctccgatgcc
     1381 tcggtgaaca gggtgccacg ctcctccatc agccgctgca gtgtcgggcc aggccacgga
     1441 ccgtaatgcc ggcccatgac tgccgcggcg ttggcacccg tcagca
//
LOCUS       DAOQYE010000641         1486 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_107676_length_1486_cov_2.993012, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000641 DAOQYE010000000
VERSION     DAOQYE010000641.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1486
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107676_length_1486_cov_2.993012"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..915
                     /locus_tag="PKN52_09735"
     CDS             <1..915
                     /locus_tag="PKN52_09735"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HNR00272.1"
                     /translation="VGARDGRLVVDDHDIGASLVLDAEGGTFEVLGTHYRGAVRLSAV
                     GDTVEVVNVLDLEAYLRGVVPAEMAASWPMEALKAQAVASRSYTLVSLDPEARYDLCA
                     TTDCQVYGGVEAEQARTDDAIARTTGVVVTYQGQTARTYYHSDSGGTVASSREVWGTS
                     YPYLVAVRDAASNTPHRAWTARLDAAVVSASLAAIGRGVGTVTSLRVLELSESGRVAA
                     LEVTGTGGATVLRGSQLTSLARGWGLKSMRFSVQSALSVRGDGWGHGVGMSQYGARAL
                     AQAGYDYGRILAYFYPTTTLTRFVAQTP"
     gene            933..1184
                     /locus_tag="PKN52_09740"
     CDS             933..1184
                     /locus_tag="PKN52_09740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00273.1"
                     /translation="MTHENRSARVAPCGRVGVNYAALLGLLVAVTFSLPMLARLAEYL
                     GLHRDAGVAVSLAIAVVAALGWFARARAATRKRSGEQDA"
     gene            1181..>1486
                     /locus_tag="PKN52_09745"
     CDS             1181..>1486
                     /locus_tag="PKN52_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNR00274.1"
                     /translation="MNKAVATGARLTLVPTPIGNLSDMTQRAVEALRGAQVVAAEDTR
                     RSRVLLDRYGITTPLERLDAHTIANRAPALLERFERVAFVTDAGTPGISDPGAELVRL
                     "
BASE COUNT          200 a          538 c          529 g          219 t
ORIGIN      
        1 gtcggcgcca gggacggcag gctcgtcgtc gacgatcacg acatcggggc ctcgctcgtc
       61 ctggacgccg agggcggcac gttcgaggtg ttgggtaccc actaccgagg agccgtgcgc
      121 ctcagcgccg ttggcgacac ggtcgaggtc gtcaacgttc tcgacctcga ggcgtacttg
      181 cggggcgtcg tgcccgccga gatggccgcg tcttggccga tggaggcgct caaggcgcag
      241 gcggtggcct cgcgctccta cacgctcgtc tcgctcgatc ccgaggcccg ctacgacctc
      301 tgcgccacga ccgactgtca ggtgtacggc ggcgtcgagg ccgagcaggc ccgcaccgac
      361 gacgcgatag cgaggacgac cggcgtcgtg gtcacgtacc aggggcagac ggcgcgcacc
      421 tactaccact ccgattccgg cggcacggtc gcttcgagca gggaagtctg gggcacgagc
      481 tacccctacc tggtggcggt acgcgacgcg gcttcgaaca cgccgcaccg ggcgtggacg
      541 gcgcggctcg acgccgccgt cgtctcggcg agcctggccg ccatcggtcg cggcgttggg
      601 acggtcacgt cgttgcgcgt cctggaactc agcgagtcgg ggcgggtcgc cgcgttggaa
      661 gtcacgggca ccggcggcgc caccgtcctg cgcggcagcc agctgacgag cctggcgcgc
      721 ggctgggggc tcaagtccat gcgcttctcc gtgcagagcg ccctgagcgt tcgcggcgac
      781 ggttggggtc acggagtcgg catgagccag tacggagcca gggccctcgc ccaggccggc
      841 tacgactacg gtcgcatcct ggcgtacttc taccccacga cgaccttgac gcgcttcgta
      901 gcccagacgc cctgagcccg accctggtag ccttgaccca tgagaaccgg agcgcccgcg
      961 tggcgccgtg tggcagggtg ggcgtgaact acgcggcgct cctcggtctg ctcgtggccg
     1021 tcaccttctc cctgcccatg ctcgcccgcc tggcggagta cctagggctg caccgcgacg
     1081 ccggcgtcgc ggtgagcctc gccatcgccg tggtggccgc gctcggctgg ttcgcccgcg
     1141 ctcgcgccgc gacgcgcaag cgttctggcg agcaggacgc gtgaacaagg ccgttgccac
     1201 gggcgcgcgg ctcacgctgg tgccgacgcc catcggcaac ctgtccgaca tgacgcagcg
     1261 cgccgtcgag gccctgcgcg gcgcgcaggt cgtggcggcg gaggacacgc gcaggagccg
     1321 agtgctcctc gaccgctacg ggataacgac ccccctcgag cgcctcgatg cccacaccat
     1381 cgccaaccgc gcccccgccc ttctggagcg cttcgagcgg gttgctttcg tgacggacgc
     1441 cggcacgccc gggatctccg accccggagc cgagctcgtg cggttg
//
LOCUS       DAOQYE010000642         1483 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_108051_length_1483_cov_2.640756, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000642 DAOQYE010000000
VERSION     DAOQYE010000642.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108051_length_1483_cov_2.640756"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="PKN52_09750"
     CDS             <1..379
                     /locus_tag="PKN52_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HNR00275.1"
                     /translation="SFLMVLSLHALRQNDLSKFRLWTVGVAVFGAIFLGFQVYEFTHF
                     AHAGLSVHQNLFGTTFFILTGTHGIHVTVGVIWLIGLVIASYKRPTSAKDAVYLEIAG
                     LYWHFVDIVWIVIFTVVYLVEFV"
     gene            431..>1483
                     /locus_tag="PKN52_09755"
     CDS             431..>1483
                     /locus_tag="PKN52_09755"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HNR00276.1"
                     /translation="MIVALILGVITYMEFALVEYPQEWLGRGWTLFLLVALSIVKFIM
                     VVLFFMHLKDDDRTYSGFFSSGMFIALATFVGLTAMFILPKAVASTRPDNQAAHEASH
                     AVPHELLENVETRGASRAPAEIADTPAVADRSVSVEAPTAPNDASTYSVTTPEGAPQG
                     VPADNAAAATEEATADTGAAAETAAGETTGTEEAAAEAATEPAATDETAAEEAPAQAG
                     QASAEAPATTEPPAASISWDKDHGAAVFNANCMACHQMTGQGIPGAFPPLADHAVDVY
                     AAPGGREYLADAVLFGLQGAINVNGMTYNGVMPAWGHLADADLADALNHVLTAWDDEP
                     AGFQPYTAADIAARRPD"
BASE COUNT          249 a          546 c          468 g          220 t
ORIGIN      
        1 gagcttcctg atggtgctca gcctccacgc gctcaggcag aacgacctga gcaagttcag
       61 gctctggacg gtgggcgtgg ccgtgttcgg ggccatcttc ctcggcttcc aggtctacga
      121 gttcacgcac ttcgcgcacg ccggcctgag cgtgcaccag aacctgttcg gcacgacgtt
      181 cttcatcctg acgggcacgc acggcataca cgtgacggtc ggggtgatat ggctcatcgg
      241 gctggtgatc gcgtcgtaca agcgcccgac cagcgccaag gacgccgtct acctggagat
      301 cgcgggcctc tactggcact tcgtcgacat cgtgtggatc gtgatcttca cggtcgtcta
      361 cctcgtggaa ttcgtgtgag cggggcgcga gagaatgtct gaaggcaagc acacctccgt
      421 aacctcctac gtgatcgtcg ctctgatcct cggcgtcatc acctacatgg agttcgcgct
      481 cgtcgagtac ccccaggagt ggctggggag gggctggacc ctcttcctcc tcgtggcgct
      541 ctcgatcgtc aagttcatca tggtcgtgtt gttcttcatg cacctgaagg acgacgaccg
      601 cacctactcg ggcttcttct ccagcggcat gttcatagcc ttggccacgt tcgtcggcct
      661 gacggccatg ttcatactgc cgaaggcggt cgcctcgacc agaccggaca accaggccgc
      721 gcatgaggcc agccacgccg tgccgcatga gctgctcgag aacgtcgaga ctcgcggcgc
      781 ctcgcgcgcg ccggccgaga tcgccgacac gccggccgtc gccgaccgca gcgtgagcgt
      841 cgaagcgccg accgctccga acgacgcgtc gacttacagc gtcaccacgc cggagggcgc
      901 gccccagggc gtgccggccg acaacgccgc cgccgcgacc gaggaagcca ccgccgatac
      961 gggcgcggcg gccgagaccg ctgccggcga gaccacgggt accgaggaag ccgcggctga
     1021 ggccgcgacc gaaccggcgg cgaccgacga gaccgccgcc gaggaggcgc ccgcccaggc
     1081 cgggcaggcc agcgccgagg cacccgccac cacggagccg ccagcggcct ccatcagctg
     1141 ggacaaggac cacggcgccg ccgtgttcaa cgccaactgc atggcgtgcc accagatgac
     1201 cggccagggc atcccgggag cgttcccacc cctcgccgac cacgccgtcg acgtttacgc
     1261 cgccccgggc ggccgcgagt acctggccga cgccgtcctc ttcggcctac agggtgccat
     1321 caacgtcaac ggcatgacct acaacggcgt catgccagcg tggggccacc tcgccgacgc
     1381 cgacctcgcc gacgccctca accacgtcct cacggcctgg gacgacgagc ccgccggctt
     1441 ccagccgtac acggccgccg acatcgccgc aaggcgcccc gac
//
LOCUS       DAOQYE010000643         1482 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_108190_length_1482_cov_2.562018, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000643 DAOQYE010000000
VERSION     DAOQYE010000643.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108190_length_1482_cov_2.562018"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1482
                     /locus_tag="PKN52_09760"
     CDS             <1..>1482
                     /locus_tag="PKN52_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00277.1"
                     /translation="AGGPWTENEFIQASTSIGIDLVACDQMTTLTTGAGLKANATHFI
                     DQTMDWDAGNQKYVQSVTYSDPLELDVPGSYSMSFQKTSRYEDRLVPNSADDVIRVEL
                     TSASLGALDLPIADHVMTLHDRALDAIGIDTADWGDHDEHGGPYYGLLLIWVQSPLSV
                     QWAQDDRGEAAPPSWWYDTFTTSPLTRQATIQAVASFGGGPSNPIRRDLAWHPTRRDE
                     NWFGEIKPGIAEADLWKWVGDWQRLSATKHLLTGEVIGGPFDMTDGDVTCGEEDPVDH
                     TAYGIMPWSEDVTDWRLYKTLRFVISSDKTEAWTLHVRLNYEYDTVTDNYMMDTEDRR
                     DGFLCEVHGNSVAYTVNVPANASSENCDIDLIEDGMPYLERVTSIELSGLPGGEDQTT
                     IHDIQLLAPTAGVWVESTDQRPVSSGGEPCYTCGSEPEFLGVAKTPEEYGGFWAMGGH
                     TIARGVRDPGRSVDVAPNWGMPFVIRRNSDCGALQDELMDFSA"
BASE COUNT          291 a          422 c          490 g          279 t
ORIGIN      
        1 atgccggggg gccgtggact gagaacgagt tcattcaggc gtccacttcc atcggcatcg
       61 acctcgtggc ctgcgaccag atgaccacgc tcacgactgg tgccggcctg aaggcgaacg
      121 cgacgcactt catcgaccag acgatggact gggacgcggg aaaccagaag tacgtgcaga
      181 gcgtgaccta ctccgacccg ctggagttgg acgtgcccgg cagctactcg atgagtttcc
      241 agaagacctc gcgctacgag gatcgactcg tgccgaacag tgcggacgat gtgatccgcg
      301 tcgagttgac gagcgccagt ctcggcgcgt tggacctgcc gatcgcggac catgtgatga
      361 ccctgcacga tcgtgccctc gacgccatcg ggatcgacac tgccgactgg ggcgaccacg
      421 acgaacacgg cgggccatac tatggcctgt tgctgatatg ggtgcagtcg ccgctctcgg
      481 ttcagtgggc acaggatgat cggggcgagg cagcgccgcc ctcgtggtgg tacgacacgt
      541 tcacgacttc tccgctcacg cggcaggcta ccatccaggc ggtcgcgtcg ttcggtggcg
      601 gtccgagcaa cccgatccgg cgcgatctcg catggcatcc aacgagacgt gacgagaact
      661 ggttcggcga gatcaagcct ggtatcgcgg aggcggacct ctggaagtgg gtcggcgact
      721 ggcagcggct cagcgcgacg aagcacctgc tcaccggcga agtgatcggc ggaccgttcg
      781 acatgacgga cggcgacgtg acctgcggcg aggaggaccc ggtagaccac acggcctacg
      841 gcatcatgcc ctggtctgag gacgtgacgg actggcgact ctacaagacg ctcaggttcg
      901 ttatctcgtc cgataagacc gaggcgtgga ccttgcatgt gcggttgaac tacgagtatg
      961 acaccgtgac cgataactac atgatggaca ctgaggaccg acgtgacggc ttcctgtgcg
     1021 aagtgcatgg taactcggta gcgtatacgg tgaatgtgcc ggcgaacgca tcatctgaga
     1081 actgtgacat cgaccttatc gaagacggga tgccatactt agagcgtgtc acgtcgattg
     1141 agttgtccgg cttgccgggc ggtgaggacc agacaaccat ccacgacatc caactgcttg
     1201 caccgacggc tggtgtgtgg gtcgagtcaa ccgaccagcg tcctgtttct tcgggcggtg
     1261 agccatgtta cacctgtggg tccgagcccg agttcttagg cgttgctaag acgcctgaag
     1321 agtatggtgg gttctgggcg atgggtgggc ataccatagc gcgtggtgtg cgtgatcctg
     1381 gccggagtgt ggacgtagcg cccaactggg gtatgccgtt cgtgatacgc cgtaatagcg
     1441 actgtggtgc gctccaggac gagctaatgg acttctccgc at
//
LOCUS       DAOQYE010000644         1482 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_108235_length_1482_cov_1.637701, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000644 DAOQYE010000000
VERSION     DAOQYE010000644.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108235_length_1482_cov_1.637701"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1021
                     /locus_tag="PKN52_09765"
     CDS             <1..1021
                     /locus_tag="PKN52_09765"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HNR00278.1"
                     /translation="ALSAWRTRDRQSEEAGMKYFLLGAFGSAMLIYGAALLYGASGSF
                     VYGDVAAALVGGNLALLATAGGALVLAGLGFKAALAPFHQWAPDVYTGAPTVVVTFMS
                     VVIKVGAFAALLRIAATVFPALQPWLLTALAVIVALTLVVGNFTALMQRGVKRMLAYS
                     SVAHAGYLGLALLAANHGGVPAAAFYLSVYALMNAGAFAVLTLVMDAGDHGDDLERFA
                     GLGKTRPGLAAAMTVFMLSLGGIPFLGGFTGKIMVFQAAIDAGHVWLAVLGIATSVVA
                     LFYYFRVVAYMYFRESAYAPHTFRSTPARVAIAVALVGTVLLGILPGWWHTLLSVGPR
                     LVAGS"
     gene            1231..>1482
                     /locus_tag="PKN52_09770"
     CDS             1231..>1482
                     /locus_tag="PKN52_09770"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNR00279.1"
                     /translation="MDLTGMRILLVDDEESALVLVRGLLTRAGYSDVRTCESPFEAAA
                     RFVEEQPDLLVIDQHMPGKDGLSVLAELGTRLPEAFPVLM"
BASE COUNT          180 a          545 c          498 g          259 t
ORIGIN      
        1 cgccctgtcc gcctggcgga cgcgcgacag gcagtccgag gaggccggca tgaagtactt
       61 cctgctcggc gccttcggct cggccatgct catctacggc gcggccctcc tgtacggggc
      121 gagcggctcc ttcgtgtacg gcgacgtggc cgccgcgttg gtgggcggca acctggcgct
      181 cctggcgacg gcgggcggcg cgctcgtcct ggccggcctc gggttcaagg ccgccctggc
      241 gcccttccac caatgggcgc ccgacgtgta cacgggcgcc cccaccgtgg tcgtcacctt
      301 catgagcgtc gtcatcaagg tcggcgcctt cgccgccctc ctgcgcatcg ccgcgaccgt
      361 gttcccggcc ctgcagccct ggctcctcac agccctggcc gtcatcgtcg ccctgacgct
      421 cgtggtgggc aacttcacgg ccctcatgca gcgcggtgtg aagcgcatgc tcgcctactc
      481 ctccgtggcg cacgccggct acctcggcct cgcgctcctg gccgccaacc acggcggcgt
      541 gccggcggcg gccttctacc tgagcgtcta cgccctcatg aacgcgggcg ccttcgccgt
      601 cctcaccctg gtgatggacg cgggcgacca cggcgacgac ctggagcgct tcgccggcct
      661 cggcaagacc aggccggggc tcgccgcggc gatgacggtg ttcatgctca gcctcggcgg
      721 catcccgttc ctcggcgggt tcacgggcaa gatcatggtg ttccaggccg ccatcgacgc
      781 cggtcacgtg tggctcgccg tgctcggcat cgccacgagc gtcgtcgcgc tcttctacta
      841 cttccgcgtc gtcgcgtaca tgtacttcag ggagagcgcc tacgcaccgc acactttccg
      901 gtcgaccccg gcgcgcgtcg ccatcgccgt cgctctggta ggtaccgtgc tgctcggcat
      961 cctgccgggc tggtggcaca cgctgctcag cgtaggcccg cgcctcgtcg caggttcctg
     1021 agctccggat ttgtgtggcc ggctggcagt ccgacagggc cgccagccgg cccgctcggc
     1081 tcggccgcgc cccgtgacgt aagaaccccg tcagcccttc gctgctaccc tcgccctgag
     1141 aggtcacgct gcccactcgt gcgcgcgtgc gggggagatg gggagtttta agcagtgtta
     1201 gcgactacag cgccatatca tgcgggggca gtggatctga cgggcatgcg catccttctc
     1261 gtggacgacg aggagtccgc gctggtgctc gtgcgcgggc tactcacgcg ggcgggctac
     1321 agcgacgtga ggacgtgcga gtcgccgttc gaggcggccg cccgcttcgt cgaggagcag
     1381 ccggacctgc tggtcatcga ccaacacatg cccggcaagg acggcctctc cgtgctagcg
     1441 gaactcggca cgcggctgcc cgaggcgttt ccggtgctga tg
//
LOCUS       DAOQYE010000645         1480 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_108431_length_1480_cov_2.891930, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000645 DAOQYE010000000
VERSION     DAOQYE010000645.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1480
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108431_length_1480_cov_2.891930"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..724
                     /gene="pgeF"
                     /locus_tag="PKN52_09775"
     CDS             <1..724
                     /gene="pgeF"
                     /locus_tag="PKN52_09775"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HNR00280.1"
                     /translation="PHGFTTRSGGASAGHYAALNLGLSSGDDRATVEGNRDTLLAHLG
                     FARGEVCCFDQVHGDRVSAGDPGWFVEEADAAVTARDDVLLVVSNADCVPLLFHDPVR
                     RVVGAAHCGWKGTAAKLAAKVVRTMAERHGSDPADVRVALGPCIRGDCYQVGEEVIAR
                     FTGGFPETVWRPDPTAGGRYLLDLPAANAFALVETGVSPEHVLDLGRCTHCEPDVFYS
                     HRRDRGLTGRHWAYVSLRGARD"
     gene            735..1361
                     /locus_tag="PKN52_09780"
     CDS             735..1361
                     /locus_tag="PKN52_09780"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HNR00281.1"
                     /translation="MPQPPTGRTSAPPVITGAELAARLDARAPRRLEIAGFRRAAVLV
                     PVLDGEAGLELLLTVRAGHLRTHAGQIAFPGGRLEEGEDVLSAALRETREEVGLDVRP
                     EQVVGVLSDHPSPAGYVATPVVALLPWPQRLAPDPSEVAEAFTVPVADLQAVAPTHRV
                     GELLRYRRRIYTYQWRDREIWGFTGNVVHDLLLALAGARAGQGDPFEP"
BASE COUNT          192 a          557 c          523 g          208 t
ORIGIN      
        1 gccgcacggc ttcacgacca ggagcggcgg cgcgagcgcg ggtcactacg ctgccctcaa
       61 cctcggcctc agctctggcg acgaccgcgc caccgtcgag ggcaaccgcg acacgctcct
      121 cgcccacctc gggttcgcgc gcggcgaggt ctgctgcttc gaccaggtgc acggggaccg
      181 cgtcagcgcg ggcgacccgg gctggttcgt cgaggaggcc gacgcggcgg tcacggcgcg
      241 cgacgacgtc ctcctggtcg tcagcaacgc agactgcgtg ccgctcctct tccacgatcc
      301 cgtccggcgc gtggtgggcg cggcgcactg cggttggaaa ggcaccgccg cgaagctcgc
      361 cgccaaggtg gtgcggacca tggccgagcg ccacggttcc gaccccgccg acgtgcgcgt
      421 cgcgctcggc ccctgcatcc gtggcgactg ctatcaggtg ggcgaggagg tcatcgcacg
      481 tttcaccggc ggtttcccgg agaccgtctg gcggcccgat ccgacggccg gcggccggta
      541 cctgctcgac cttcccgccg ccaacgcgtt cgccctcgtc gagaccgggg tctcgcccga
      601 gcacgtgctg gatctaggca gatgcacgca ctgcgagccc gacgtcttct acagccaccg
      661 gcgcgaccgc gggctcacgg gaaggcactg ggcctacgtg tcgctccgcg gtgcgcggga
      721 ctaacatggc ggtcatgccg caaccgccca ccggtcggac gagcgctcct cccgtcatca
      781 ccggagcgga gctcgccgcc cgcttggacg cccgcgcacc gcgtcgcttg gagatcgccg
      841 ggttcaggcg cgccgccgtc ctggtgcccg tcctcgacgg cgaggccggc ctcgaactgc
      901 tgctgaccgt acgtgccggc cacctgcgca cccatgccgg ccagatcgcg ttcccgggcg
      961 ggcgcctcga ggagggcgag gacgtgctgt cggcggcctt gcgcgagacg cgcgaggagg
     1021 tcggcctcga cgtgcgcccg gagcaggtgg tcggggtgct ctcggatcac ccgtcgccag
     1081 ccggctacgt cgccacgccc gtcgtcgcgc tcctcccgtg gccgcagcgc ctcgcacccg
     1141 acccgtcgga ggtcgccgag gcgttcaccg tgcccgttgc cgacctccag gccgtcgccc
     1201 cgacccaccg cgtcggggag ctgctgaggt acaggcgcag gatctacacc taccagtggc
     1261 gcgaccgtga gatatggggg ttcacgggca acgtcgtgca cgacctgcta ctggccttgg
     1321 caggagcgcg ggcggggcag ggcgacccct tcgagccgtg aagacccgtg agctcagggt
     1381 cgtcaggggc gaggagcgct actccttctc caccggcgct ctggagggcc atcgacctcg
     1441 cgctcgacgt cgaggcgcag ctgcgcgaca gcccctcgcg
//
LOCUS       DAOQYE010000646         1479 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_108557_length_1479_cov_2.888343, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000646 DAOQYE010000000
VERSION     DAOQYE010000646.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1479
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108557_length_1479_cov_2.888343"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(135..773)
                     /locus_tag="PKN52_09785"
     CDS             complement(135..773)
                     /locus_tag="PKN52_09785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00282.1"
                     /translation="MARVLAIADAVSPVVYSSNFPANLAPFDLVLSAGDMSGAVLEFV
                     ATKTSTPLLYVFGNHAVGYIRDDVTDEARPPGGCTNVHMRLVKAAGLLVVGFEGSVRY
                     RPGPHQYSQLVYYLMVWRMAPRLLWSRLRNGRAVDVLLTHAAPVGPNAGSDHVHVGVP
                     AFNLFHRLFRPRVHVHGHVHLSGANAPRSYVDPTGVRVVNAFEFARLDVEGG"
     gene            complement(781..>1479)
                     /locus_tag="PKN52_09790"
     CDS             complement(781..>1479)
                     /locus_tag="PKN52_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HNR00283.1"
                     /translation="AGMLLRMYRRFAERHGMQVELLDLVPNHDAPNGIDYAQLIVRGE
                     RAFGMLRMEGGVHRLVRVSPFDSQNRRHTSFASVEIMPEIDDTFELAIDQKDVRVDVY
                     RSSGPGGQSVNTTDSAVRVVYKGGTPEEVVVTIQDGKSQIKNREKAMTVLRSRLFERE
                     EQRRLDEQLKARGEQKAIEWGSQIRSYVLDKQYVKDHRTGGMRHDPDNVLDGDIEDLV
                     WAGLEWAAKNELVA"
BASE COUNT          235 a          497 c          507 g          240 t
ORIGIN      
        1 cgaccggcaa cccggccacg tcgttggcta gctggccctg caggaagccg gtggcgtccg
       61 gaccctcgac gctcagcgtc acgacccccg cgacctcacc cgccaccggt ggcgatgttc
      121 gactcatgcc aacatcatcc cccttccacg tcgaggcggg cgaactcgaa cgcgttgacg
      181 acccggactc ccgttggatc gacgtagctg cgcggggcgt tggcccccga aaggtggacg
      241 tgcccatgaa cgtgcaccct cggcctgaac aggcggtgga agaggttgaa agccggtacg
      301 ccgacgtgga cgtggtcgct gccggcgttc gggcccacgg gcgcggcatg ggtcagtagg
      361 acatcgacgg cgcggccgtt gcgcaaccgg ctccagagga ggcgcggtgc catccgccac
      421 accatcaggt agtagacgag ctgagagtac tggtgcgggc cgggtcggta ccgaacgctg
      481 ccctcgaagc cgacgacgag gagcccggcc gccttcacga ggcgcatgtg cacgttggtg
      541 cagccgccgg gcggccttgc ctcatccgtg acgtcgtccc tgatgtagcc gacggcgtgg
      601 ttcccgaaga cgtagagcag cggggtgctc gtcttggtcg ccacgaactc gagcacggca
      661 ccggacatgt cgccggcgct gaggacgagg tcgaacggcg ccaggttggc ggggaagttg
      721 ctcgaataga cgaccggtga gacggcgtcg gcgatggcca gtacccgggc catcgccgcg
      781 ctaggccacg agctcgttct tggccgccca ctcgaggccc gcccacacga ggtcctcgat
      841 gtcgccgtcc aagacgttgt ccgggtcgtg gcgcatgccg cccgtgcggt ggtccttcac
      901 gtactgcttg tcgagcacgt aggagcggat ctgcgagccc cactcgatgg ccttctgctc
      961 gccgcgcgcc ttgagctgct cgtcaaggcg ccgctgctcc tcgcgctcga acaggcgcga
     1021 ccgcaacacg gtcatggcct tctcgcggtt cttgatctgc gacttgccgt cctggatggt
     1081 gacgacgacc tcctcgggcg tgccgccctt gtagacgacg cgcaccgcgg agtcggtggt
     1141 gttcacggac tgcccaccgg ggccgctgga gcggtagacg tcgacgcgca cgtccttctg
     1201 gtcgatggct agctcgaacg tgtcatcgat ctccggcatg atctcgacag acgcgaacga
     1261 cgtatgccgg cggttctgcg aatcgaacgg gctcacgcgc acgaggcgat ggacgccacc
     1321 ctccatgcgc agcatgccga acgcgcgctc gccgcgcacg atgagctggg cgtagtcgat
     1381 gccgttgggc gcgtcatggt tgggcaccag atcgaggagt tccacctgca tgccgtggcg
     1441 ctcggcgaag cgccggtaca tgcgcagcaa catgccggc
//
LOCUS       DAOQYE010000647         1478 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_108714_length_1478_cov_2.042867, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000647 DAOQYE010000000
VERSION     DAOQYE010000647.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1478
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108714_length_1478_cov_2.042867"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..683)
                     /locus_tag="PKN52_09795"
     CDS             complement(84..683)
                     /locus_tag="PKN52_09795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00284.1"
                     /translation="MRRLLRTALACIAVAALAVAAAQSGAVMSGIVKSDEPLADNTRV
                     AIHVVDSDNVWGLEVATVAPVGGTFRVSPGPVPADQLRPFRSGSVILPGLQNEYRVSP
                     EGVNVAVARFNMYVDQNGNDVFDRVVDRFYIGVASLEAPVGFFTLLYVDQAATLTGGG
                     VELPLRQGWNVFTVRFPGDKAEYAIVPAVEDIVLDVVLQ"
     gene            complement(835..>1478)
                     /locus_tag="PKN52_09800"
     CDS             complement(835..>1478)
                     /locus_tag="PKN52_09800"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNR00285.1"
                     /translation="NEEAVIEGVVRTLVDQNYPPELFDIWVVPNGCTDDTERVAREAG
                     ATGLASAALRYFNAAGATASLGEDHRPESHLIPIVLQVAAGKREQAQVFGTDYDTPDG
                     SAVRDYVHVSDLAEAHALALEAMRPGEARAYNLGSGSGYSVREVIDVCRAVTGHAIPT
                     AEAPRRAGDPPRLVADSSLARSELGWKPRFDDLGTLVASAWEWHSAHPHGYAD"
BASE COUNT          208 a          510 c          526 g          234 t
ORIGIN      
        1 ggcggaactg gcgctactcc gctacgccgc cgcccagggc tggacgcgcg agcgcgaggc
       61 gtgagggccg ggaaggcccg cgcttactga agtacgacgt ccaggacgat gtcttctacg
      121 gccggcacga tggcgtactc ggctttgtcg ccggggaagc gcaccgtgaa cacgttccac
      181 ccctggcgaa gtggcagttc gaccccgccg cccgtgagag tggcggcctg gtcgacgtag
      241 aggagcgtga agaagccgac gggcgcctcg aggctcgcta cgcctatgta gaagcggtcg
      301 acgacccggt cgaacacgtc gttgccgttc tgatcgacgt acatgttgaa gcgggcgacc
      361 gcgacgttga cgccttccgg gctgacccgg tactcgttct gcagacccgg caggatgacg
      421 ctgcctgagc ggaacggccg gagctggtcg gccggcaccg ggccggggct cactcggaac
      481 gtgcccccga ccggcgctac ggtcgcgacc tcgagccccc aaacgttatc gctgtcgacg
      541 acgtggatgg ccacacgggt gttgtctgcg agcggctcgt cgctcttcac gatgccgctc
      601 atcaccgcgc cagactgcgc tgccgccacg gccagggcgg ccacggcgat gcaggcaagg
      661 gcggtgcgga gtaatcgtct catgacagtc tccaaatcgg aggcagcggg cttcctgggc
      721 gacgcaagtt cgcctccatg tcgcaacagg tcatatcaca tcttcggcgc acgtcggtgc
      781 ctggtggatg agcgcgggga tcggggcgtt caccgctcgc tcatcacgca cgcgtcagtc
      841 cgcgtacccg tgggggtggg ccgagtgcca ctcccacgcg ctcgccacca gcgtcccgag
      901 atcgtcgaac cgcggcttcc agccgagttc gctgcgggcg agcgagctgt cggccacgag
      961 ccgcgggggg tcgcccgccc ggcgcggggc ctccgcggtc gggatggcgt ggcccgtgac
     1021 ggcgcggcac acgtcgatca cctctcgcac cgagtaaccg ctgccgctgc ccaggttgta
     1081 ggcgcgcgcc tcgcccggtc gcatggcctc gagggcgagg gcgtgggctt ccgccaggtc
     1141 gctcacgtgg acgtagtccc gcacggcgct gccgtcgggc gtgtcgtagt cggtgccgaa
     1201 cacctgggcc tgctcgcgct tgcctgcggc cacctgcagc acgatgggga tcagatggct
     1261 ctcgggccgg tggtcctcgc cgaggctcgc ggtcgctccc gccgcgttga agtacctgag
     1321 ggcggccgag gcgaggccgg tcgccccggc ctcgcgcgcg accctttcgg tgtcgtccgt
     1381 gcagccgttc ggcacgaccc agatgtcgaa cagctccggc gggtagttct ggtcgacgag
     1441 cgtccggacg acgccctcga tgaccgcctc ctcgttgc
//
LOCUS       DAOQYE010000648         1473 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_109282_length_1473_cov_2.536671, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000648 DAOQYE010000000
VERSION     DAOQYE010000648.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1473
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109282_length_1473_cov_2.536671"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            215..649
                     /locus_tag="PKN52_09805"
     CDS             215..649
                     /locus_tag="PKN52_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNR00286.1"
                     /translation="MVSRSEAVGGRAADASAGRGASIRRCAAGSAIEILQEKWVLHIV
                     HALLGGSRCFNELGRDVGGCNPTTLAHRLARLEGVGVIRKEVLHDDAHAAGLPRCSYE
                     LTDAGRELQEVIDAIRVWAVAHLGTSEPNGDPGYFDGDAGRT"
     gene            complement(790..>1473)
                     /locus_tag="PKN52_09810"
     CDS             complement(790..>1473)
                     /locus_tag="PKN52_09810"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNR00287.1"
                     /translation="DDLQLQLVDVLLSDPRFHLARAASARAALEYLKANTPDLIIAAA
                     DLPDLDGYALVSRVKRVVRLAHVPVILTTEADAGLGLSQRTRRRAEEAQVDLLLPRPL
                     GDKNLKERALRLLQQGGSGRKRADQPRLATTLVLEEALERLPLPPPHPTRPAAPGSSD
                     VVAGGAAAQPNAAQAQVAQAHLARAERESLIRENEALRNENRELRASIERLRRELNEA
                     RQVGRPGKR"
BASE COUNT          186 a          518 c          525 g          244 t
ORIGIN      
        1 atgtagctcc cttcgggcga acctaccgct cctggcagta gccttcaggc cgccttaccc
       61 cctggacgcc ggtggtggct gccgcagccg cgctagcgcc cgggaagtca ttttaccatc
      121 acgccgcgga tcggccggac gtggctaggt ggaggccggt gtcgtgtagg ccgcgctaaa
      181 ccccacaacc ccggacggtg gtatcgtcgt tgacgtggtg agtcggagcg aggctgtcgg
      241 cggtcgtgcc gccgatgcga gtgcagggcg cggcgcgtct atcaggcgct gcgcggccgg
      301 ttccgccatc gagatccttc aggagaagtg ggtcctgcac atagtgcacg cgttgctcgg
      361 tggctcgcgc tgcttcaacg agctggggcg cgatgtcgga ggctgcaacc cgaccaccct
      421 ggcgcaccgc ctcgcccgtc tcgagggcgt cggggtgatc cgcaaggaag tgctccacga
      481 cgacgctcac gccgccggcc tgccgcgctg cagctacgag ctcaccgatg ccggtaggga
      541 gctgcaggaa gtcatagacg ccatccgggt atgggccgtc gcccatctcg gaacgtctga
      601 gccgaacggc gatcccggtt acttcgacgg cgacgccggc cgcacctgac gctgccgatt
      661 gtaggcggcc gctccggccc gcgccccgcc tgtgctttgc tctgagccgc gcttgcccgg
      721 actagcgttt ccgcggcctg agccccctta cgacccctgg cgcttactta tccgtctaac
      781 ccttgccgcc tagcgcttgc cgggcctgcc gacctgccgc gcctcgttca actcgcgcct
      841 caggcgttcg atgctcgcgc gcagctcgcg gttctcgttg cgcagcgcct cgttctcgcg
      901 gatcagcgac tcgcgctcgg ccctggcgag gtgggcctgc gccacctggg cctgcgcggc
      961 gttgggctgc gcggcggccc cgcccgccac cacgtccgag gaccccggcg cggcgggtcg
     1021 cgtggggtgg ggcggtggaa ggggcagacg ctccagcgcc tcctccaaca cgagggtcgt
     1081 ggcgagccgg ggctggtcgg cccgcttccg gccagaaccg ccctgctgca gtagtcgcag
     1141 cgcgcgctcc ttcaggttct tgtcgcccag cggccggggc aggagcaggt cgacctgtgc
     1201 ctcttccgct cgccgcctcg tgcgttgcga gaggccaagg cccgcgtcag cttccgtcgt
     1261 caggatgacc gggacgtgcg cgaggcgcac gacccgcttg acgcgcgaga cgagcgcgta
     1321 gccgtccagg tcgggcaggt cggcggccgc gatgatcagg tccggggtgt tggccttcag
     1381 gtactcgagc gcggccctgg cgctggccgc gcgcgccagg tggaagcggg ggtccgagag
     1441 gaggacatcc acgagttgga gctgcaggtc gtc
//
LOCUS       DAOQYE010000649         1472 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_109388_length_1472_cov_3.215243, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000649 DAOQYE010000000
VERSION     DAOQYE010000649.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1472
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109388_length_1472_cov_3.215243"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            273..>1472
                     /locus_tag="PKN52_09815"
     CDS             273..>1472
                     /locus_tag="PKN52_09815"
                     /inference="COORDINATES: protein motif:HMM:NF039484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00288.1"
                     /translation="MALLAVASRPWAASPALAWVSFLPVFRLVRVLAARRRFLSAAAC
                     GAFTATGVTAVAYEAALGVSVPAYLVAVVAAALPFGAACGMAAFVLARLRGAGRRLAW
                     LVPPVLWCCAELSIRQEWLAGAWAPALAVIGYSQVGTPAAQLARIGSVTAVSLAVLLA
                     NALALALLEAIRLAVASVGSGGSSLLGRARPVLPPVAGLGLLWLVIWWAAAAAPAVAA
                     AQTAHGAGTRIVVVQPNLPSAVRAAAAADAALAAELLAAMAALTGPGGAAVPALAVWP
                     EGVWPGRLSHAPDAADAASVARPGSYSRTALPGLPPLLLGAASRDESTGAYGNSAFAF
                     TGSDLVHVSDKSRLVPFAEAGMAPGATPGPVELGGVLAAPVICYDVAFPATVRAAARS
                     GAELLAVM"
BASE COUNT          122 a          561 c          541 g          248 t
ORIGIN      
        1 gacccggcca tcggcgccta cgtgatcccg ctggaagcct tcgcgcgccg cttcagcggc
       61 cgcgtgttcc tgccggaggc ggcgtcgtga cgctcgttcc ttccaccctg ccggtgcgcc
      121 gagctctacg gtcgcgcgtc gccgcaccgc gcgttgcgtc ggcattcggc gcaccggcac
      181 ccgacgcacg ggcttcggcg ccgaaccgct catcaccgaa gggcgcgccg gccggcatcg
      241 gggcggtcga gtacctaact tgcctcgccg gcgtggcgct cctagccgtc gcctctcggc
      301 cgtgggccgc gtcgcccgcg ctggcctggg tctccttcct gcccgtcttc cgccttgtgc
      361 gggtcctggc ggcgcgccgc cgcttcctga gcgcggcggc ctgtggcgcg ttcaccgcga
      421 ccggcgtcac cgccgtggcg tacgaggcgg cgttgggcgt ctccgtgccc gcatacctcg
      481 tagcggtcgt ggcagcggcg cttccgttcg gagcggcctg cggcatggcg gctttcgtcc
      541 tcgcgcgctt gcgtggggct ggtcggcgcc tagcgtggct cgtcccgccc gtgctctggt
      601 gctgcgctga gctgtctatc cgacaggagt ggttggcggg ggcatgggcg ccggcactgg
      661 ccgtgatcgg ctactcccag gtcgggacgc ccgccgccca gctggcgcgc atcggctcgg
      721 tgacggcggt gagcctggcc gtcctgctcg ccaacgccct ggctttggcg ctgctcgaag
      781 ccatccgctt ggcggtggcg tccgtcggct ccggtgggtc gtcgctcctc ggccgggcgc
      841 ggcccgtgct cccgccggtc gcgggtctcg ggctcctctg gctcgtcatc tggtgggcgg
      901 cggccgccgc gccggcggtc gcggcagcgc agacggccca cggcgccggc acgaggatcg
      961 tcgtggttca gccgaacttg ccgtccgccg tgcgagccgc ggccgcggca gacgcagcct
     1021 tggccgccga gctccttgcc gccatggccg cgctgaccgg cccgggcggc gcggcggtgc
     1081 cggcgctagc cgtgtggccg gaaggggtct ggcccgggcg gctaagtcat gcgcccgacg
     1141 ctgcggatgc ggcctccgta gctcggccag ggagctactc gcgcacggcg ttgcccggtc
     1201 tgcccccgct gctgctcggc gcggcctctc gcgacgagtc gaccggagcg tacggcaact
     1261 ccgccttcgc cttcaccggt tccgacctgg tccacgtctc agacaagagc cgcctggtcc
     1321 cgttcgcgga agccggtatg gcgccaggcg ctactcccgg ccctgtcgag ctaggcggcg
     1381 tcctggccgc cccggtgatc tgctacgacg tcgccttccc ggccacggtg cgcgccgccg
     1441 cgcgttccgg cgcggagctg ttggccgtca tg
//
LOCUS       DAOQYE010000650         1470 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_109644_length_1470_cov_3.120848, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000650 DAOQYE010000000
VERSION     DAOQYE010000650.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1470
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109644_length_1470_cov_3.120848"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..804)
                     /gene="pyk"
                     /locus_tag="PKN52_09820"
     CDS             complement(<1..804)
                     /gene="pyk"
                     /locus_tag="PKN52_09820"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HNR00289.1"
                     /translation="MKQRRCTKIVATLGPVSSSPDVIQNLLEAGVNVFRLNFSHGAQE
                     VHRATVDTIRTTAEKLGVSVGILQDLQGPKIRVARFKDDHVTLVPGQEFTLTCGDSSP
                     GDGTRVGVTYTNLVNDLKVGDMLLLDDGRLELRVTGIQDDDIHTEVVVGGVLSNNKGI
                     NIPGAHLSIPALTDKDVEDLRFGAELDVDWVAMSFVRTRDDLLLARHYLARAGSQARL
                     MAKIEKPSAVERFSEILQEVDGIMVARGDLGVEMPAERVPLIQKQLIREC"
     gene            complement(801..>1470)
                     /gene="eno"
                     /locus_tag="PKN52_09825"
     CDS             complement(801..>1470)
                     /gene="eno"
                     /locus_tag="PKN52_09825"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176526.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HNR00290.1"
                     /translation="DEGGFAPNLKSNDEAFEVLLEAIEKAGYTPGDEIGIALDPASTE
                     FFADGRYVLKAEGQEFDSDGMIAYWLDWIERYPIVSIEDGLAEDDWVGWAKLTQAAGT
                     RVQLVGDDLFVTNSARLQRGIDEAVGNAILVKVNQIGTLTESMDAMELAKTYGYRNVV
                     SHRSGETEDAFIADLAVAVNAGQIKTGSASRSDRIAKYNRLLAIESELGDAARYLGKA
                     AFKR"
BASE COUNT          217 a          481 c          492 g          280 t
ORIGIN      
        1 gcactcgcgg atgagctgct tctggatgag cggcacccgt tccgccggca tctcgacgcc
       61 aaggtcgccg cgggccacca tgatgccgtc cacctcttgc aggatctcgc tgaagcgctc
      121 gacggcgctc ggcttctcga tcttggccat gaggcgcgcc tgggaacccg cgcgggccag
      181 gtagtgccgc gccaggagga ggtcgtcgcg ggtgcgcacg aagctcatgg ccacccagtc
      241 gacgtccaac tccgccccga agcggaggtc ctccacgtcc ttgtctgtca aggcggggat
      301 gctgaggtgg gcgccaggga tgttgatgcc cttgttgttg ctgagcaccc ccccgacgac
      361 cacctcggta tggatgtcgt cgtcctgaat gcccgtgacg cgcaactcca ggcgaccgtc
      421 gtcgagcagg agcatgtcgc cgaccttgag gtcgttgacc aggttcgtgt aggtgacgcc
      481 gacccgcgtc ccgtcgccgg ggctcgaatc gccgcacgtg agggtgaact cctggcccgg
      541 aacgagggtg acgtggtcgt ccttgaagcg ggccacgcgg atcttcggac cttggaggtc
      601 ctgcaggatg ccgacggaca caccgagctt ctcggccgtg gtgcggatgg tgtcgaccgt
      661 cgcgcggtgt acttcctggg cgccgtgcga gaagttgagg cggaagacgt tgacgcccgc
      721 ctcgagcagg ttctggatca cgtccggcga gctcgacacg gggcccaagg tagctacgat
      781 cttggtgcag cgcctctgct tcatcgcttg aaggccgcct taccgagata ccgcgccgcg
      841 tcccctagtt cgctctcgat ggcgagtagc cggttgtact tggcgatacg gtcggagcgg
      901 ctcgcggacc ccgtcttgat ctggcccgcg ttgactgcca cggcgaggtc ggcgatgaag
      961 gcgtcctccg tctcacccga ccggtggcta acgacgttcc ggtaaccgta ggtcttggcg
     1021 agttccatgg cgtccatgga ctccgtgagc gtcccgatct ggttgacctt cacgaggatg
     1081 gcgttgccga cggcctcgtc gatgccgcgc tgcagacggg cgctgttcgt cacgaacagg
     1141 tcgtcgccga cgagctgcac gcgcgtgccg gctgcctgcg tcagcttcgc ccagcccacc
     1201 cagtcgtcct ccgcgagtcc gtcctctatg gagacgatgg ggtagcgctc gatccagtcg
     1261 agccagtagg cgatcatgcc gtcggagtcg aactcctgac cctcggcctt gagtacgtac
     1321 ctgccgtcgg cgaagaactc cgtgctggcg gggtcgaggg cgatgcctat ctcgtcgccc
     1381 ggcgtgtaac cggccttctc gatggcctcc aagaggacct cgaaggcctc gtcgttgctc
     1441 ttgaggttcg gcgcgaagcc accctcgtcg
//
LOCUS       DAOQYE010000651         1468 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_109885_length_1468_cov_2.542109, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000651 DAOQYE010000000
VERSION     DAOQYE010000651.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1468
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109885_length_1468_cov_2.542109"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="PKN52_09830"
     CDS             complement(<1..366)
                     /locus_tag="PKN52_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HNR00291.1"
                     /translation="MSESIFHLGTGPVHVSAEQIAGRVKELGAEIAADHPTGELHLIC
                     VLNGAFMFMADLVRAIDRPLTVDFLSVSSYGSRTESSGEVKLVKDLDQSLKDRQVVLV
                     EDIVDTGLTMQYLLTYLKGR"
     gene            complement(518..>1203)
                     /locus_tag="PKN52_09835"
     CDS             complement(518..>1203)
                     /locus_tag="PKN52_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNR00292.1"
                     /translation="ARAGIASRRAAEDLIRRGRVRINGRVAQLGDRVEPGDDVRVDGK
                     QVSEPQDHVTYVLNKPAGYVTTARDERGRATVFDLLPPTPGLHAVGRLDRDSEGLLLL
                     TTDGDLTLRLTHPRYELEKEYRVWTKEGRLDGITLKKLLAGIELDDGIAAARSARPAP
                     GGAVVVLTEGRKREVRRLFKVLGYPVERLVRVRVGTLKLGDLAPGAFRELSEKELRSL
                     GHVPMPRGA"
     assembly_gap    1204..1213
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          179 a          529 c          500 g          250 t
ORIGIN      
        1 gcgacccttg aggtaggtga gaaggtactg catggtcagg cccgtgtcga cgatgtcctc
       61 gaccagcacg acctgccggt ccttgaggct ctggtcgagg tccttgacca gcttcacctc
      121 gccgctcgac tccgtgcgcg agccgtacga ggagacggac aggaagtcga cggtcagggg
      181 tcggtcgatg gcgcgcacca ggtcggccat gaacatgaag gcgccgttca ggacgcagat
      241 caggtgcagc tcgcccgtag ggtggtcggc cgcgatctcg gcgccaagct ccttgacgcg
      301 cccggcgatc tgctcggcgc tcacgtgcac gggacccgtc cccaagtgga agatgctctc
      361 gctcacctag aactccagtt ccttgggggc gacgggcgcc cccaagcgcg gctacggccg
      421 cgcgcgaccc cataggctac cgccccgcgc cgaccgtggt gccggatggg ccgccccatg
      481 gggcggcgtg cggtccccgg ttcaggagcc gatccggtca ggcgccgcgc ggcatcggaa
      541 cgtgaccgag cgagcgtagc tccttctcgc tcagctcccg gaacgccccc ggggcaaggt
      601 cgccgagctt cagggtcccg acacggaccc tgacgagccg ctccaccggg tagccgagca
      661 ctttgaacag gcgcctcacc tcgcgtttac gcccctcggt caggaccacg acggccccgc
      721 ccggcgccgg gcgggcgctg cgggcggcgg cgatgccgtc gtcgagctcg atgccggcga
      781 ggagcttctt caacgtgatg ccgtcgagcc gcccctcctt cgtccacacc cggtactcct
      841 tctcaagctc gtagcgcggg tgcgtgaggc ggagcgtgag gtcgccgtcc gtcgtgagca
      901 gcaacaaccc ctccgagtcg cggtccagtc ggcctaccgc gtgcagcccg ggcgtcggcg
      961 ggaggaggtc gaagacggtg gcgcgcccgc gttcgtcgcg cgccgtcgtg acgtagcccg
     1021 ccggcttgtt gaggacgtag gtgacgtgat cctgcggctc gctcacctgt ttgccatcga
     1081 cgcgcacgtc gtcaccgggc tcgacgcggt cgccgagctg ggccacgcgg ccgttgatgc
     1141 gcacgcgtcc gcgccggatg aggtcctccg ccgcgcgccg gctggctatg ccggctctgg
     1201 ctannnnnnn nnncccgttg cgcatgtcct cggccggggg tccgctgggc cgttggcccg
     1261 gcacccgtcg gcctcttgcc cgtcggtcgg ttacgtgtcg gcccgttgcc tgccggtctg
     1321 ttgcccgccg gtcggttacc tgccgacctc ttgcccgccg gtccgttgcc tgccggcttc
     1381 ttgcccgccg gcttcttgcc cgccggttta ctactcgctg gtctcggccg gcgcttctgg
     1441 tctcgatcgt cgctcatccc agagcatg
//
LOCUS       DAOQYE010000652         1468 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_109897_length_1468_cov_2.337580, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000652 DAOQYE010000000
VERSION     DAOQYE010000652.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1468
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109897_length_1468_cov_2.337580"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="PKN52_09840"
     CDS             complement(<1..315)
                     /locus_tag="PKN52_09840"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HNR00293.1"
                     /translation="MKAIVVDRNRPGRPLLWQEVADPSCGEDDVIVDVQATALNRADL
                     LHREGNFPTTAGRSDLLGGEDAGRIREVGNAVQGWSIGDSVVCLTLGGGYAEQVRVPQ
                     RLL"
     gene            590..>1468
                     /locus_tag="PKN52_09845"
     CDS             590..>1468
                     /locus_tag="PKN52_09845"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNR00294.1"
                     /translation="MKSLRFAVSYRLLVAVGLALCCLLPSAAAQQAGGKLTVAIGVPA
                     VTLAPGKSIASIVNSITEGIYEGLVERDASGAVLPGLATSWSLDADNVTWTLTLRQGV
                     RFHDGTEFSADDVKATFDRILNPAFELPLSSVLKVVSRVEVVDPYTVKIVTGSPTPDF
                     LNRLAYGVAVIISSDAVEKYGADIDWTPVGTGPYRLESYVPNESVTLVAFPDYWGGTP
                     LLDRVEFRTVREPGTRVALLEAGEVDIIVDVPATDLPGLKAQADKAVLLAPSTRVMHI
                     GVNTQVAPFDNVLVRQA"
BASE COUNT          235 a          503 c          470 g          260 t
ORIGIN      
        1 gagcagccgc tggggcacac ggacctgctc ggcgtagccc ccgcccaggg tcaggcagac
       61 cacgctgtcg cctatcgacc atccctggac ggcgttgccc acttccctga tgcgcccggc
      121 gtcttcccca cccaggaggt cggagcgccc ggcggtcgtc gggaagttac cttcgcggtg
      181 cagcaggtcg gctcggttga gcgcggtggc ttggacgtcg acgatcacgt cgtcctcgcc
      241 gcacgaggga tcggccacct cttgccatag gagggggcgg ccaggccggt tccggtccac
      301 gacgatcgcc ttcatctgcc tctgaacctc gctctccgct tatgcggcct ggatgctagc
      361 acaggctgac ggaccgacga ctcggtcaga gagaaacgtt gttctgtacg gaatatctcc
      421 gatcggaagg ggcttggggc gccgccattc tctcccatat gttgggacta tcatccttgt
      481 agttgacatg catgatgggc gcctctatag tggcgcgcac gtacagcgga gcaagccggc
      541 gccgcggcgt actgtgacca ttcggttcag caacgcaagg gaggaacgga tgaagtccct
      601 caggttcgcc gtctcgtatc gcctattggt ggcagtgggg ctcgcgctgt gctgcctcct
      661 tccgtctgcc gccgcgcaac aggcgggcgg gaagttgacc gtggccatcg gcgtgccggc
      721 cgtgaccctt gccccaggca aatcgatcgc gtcgatcgtc aacagcatca cggaaggcat
      781 ctacgaaggc ctggtggagc gggacgccag cggcgcggtc ctccccggtc tggcgacctc
      841 gtggagcctc gacgcggata acgtcacctg gaccctcacc ctgcggcaag gcgtccggtt
      901 ccacgacggc accgagttct ccgccgatga cgtcaaggcc accttcgacc gcattctgaa
      961 ccccgcgttc gagctgccgc tcagctccgt cttgaaggtc gtctcgcgcg tcgaggtagt
     1021 ggacccttac acggtcaaga tcgtcaccgg cagtcccacg ccggacttcc tgaaccgcct
     1081 ggcctacggc gtagccgtca tcatcagcag cgacgcggtc gagaagtacg gagccgacat
     1141 cgactggacg ccggtcggca ccggtccgta ccgcctcgag tcctacgttc ccaacgagag
     1201 cgtcacgctc gtcgcgttcc ccgactactg gggcggcacc ccgttgctcg atcgcgtcga
     1261 gttccgcacg gttcgcgagc cgggcacgcg cgtcgcgctg ctcgaggcgg gcgaggtcga
     1321 catcatcgtc gacgtgccgg ccaccgacct gcctggcctg aaggcgcagg ccgacaaggc
     1381 cgtgctgttg gcgccgagca cgcgagtcat gcacatcggc gtcaacaccc aggtggcgcc
     1441 tttcgacaac gtcctcgttc gccaggcg
//
LOCUS       DAOQYE010000653         1466 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_110113_length_1466_cov_2.830617, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000653 DAOQYE010000000
VERSION     DAOQYE010000653.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1466
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110113_length_1466_cov_2.830617"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            150..608
                     /locus_tag="PKN52_09850"
     CDS             150..608
                     /locus_tag="PKN52_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00295.1"
                     /translation="MRKFLVSLIAVAALGAGAAFAQGGMWAGVSAGYPGAALHFGIDD
                     VAPNLAVRLNAGYNYIGTAGFALGADVLYDLDVDLGDTPIQVYVGGGVGLGIGSGIAL
                     GINAFVGGEFSLASAGLPQGGVFLEVGPAIAVVPSFGFGVTGRLGFNYHF"
     gene            complement(734..>1466)
                     /locus_tag="PKN52_09855"
     CDS             complement(734..>1466)
                     /locus_tag="PKN52_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HNR00296.1"
                     /translation="QLYQLRGRVGRRQTEAWAYMLYPGRLTEQAQRRLYAIAELNDLG
                     SGHLLAEKDMEIRGVGNLLGPEQHGHISAVSIAVYTEMLAEEVAKLKGELRAAEVPQV
                     TVDLAVDARLSPAYIQDDAVRINYYGRLASTGGLAEVNRIAKELREAYGPFPPEVRAF
                     IELTRLRLVAGAKGVATIKEHMTDVQVALAADVEAIDYDAKRLKALPFQVEPTRYPPG
                     FSVKKRGLKDFEVPGALLELLYLVG"
BASE COUNT          204 a          527 c          479 g          256 t
ORIGIN      
        1 gcgcgttcgc ggagcgcagc gacggaaaac ccgatgccca tcggatatga gtgaaaaccc
       61 cgacttgtcg cccagaaaag cccgtgatat aaccgggcag gcgaggagaa cgtaatccta
      121 gtcttctcag atcggtttgg agggttcgaa tgcgcaagtt cttggttagc ctgatcgccg
      181 tcgccgccct gggtgccggt gcagccttcg ctcaaggcgg tatgtgggcc ggcgtcagcg
      241 ccgggtaccc aggtgccgcg cttcacttcg gtatcgacga cgtcgcgccg aacttggctg
      301 tacgactcaa cgcgggctac aactacatcg gaaccgccgg cttcgcgctc ggcgccgatg
      361 tcctttacga cctcgacgtc gacctgggcg acacgcccat ccaggtctac gtcggcggcg
      421 gtgtcggcct cggcatcggc tcgggcatcg cgctcggcat caacgccttc gttggtggcg
      481 agttcagcct ggccagcgcc ggcctcccgc agggcggcgt tttcctcgaa gtcggccccg
      541 ccatcgccgt cgtgccgtcc ttcggcttcg gcgtcaccgg ccgcctcggc ttcaactacc
      601 acttctaagc accagcttcg cgcttagtcg cggcgccccg cggcccatcc ggccgcgggg
      661 cgcgctcttt ctcggcgggc tggccggacg cggggcgagc ctcgacgtcc gaccgacgcg
      721 ccgcggccag ccctcagccg accaggtaca gcagctctag cagcgcccct ggcacctcga
      781 agtccttcag cccgcgcttc ttgacgctga agccgggagg gtagcgcgtc ggctcgacct
      841 ggaacgggag cgccttcagg cgcttggcgt cgtagtcgat ggcctcgacg tcggccgcca
      901 gggcgacctg aacgtccgtc atgtgctcct tgatcgtcgc tacgcccttg gcccccgcca
      961 ccaggcgcag gcgcgtgagc tcgatgaacg cccgcacctc gggcgggaac ggcccgtacg
     1021 cctccctcag ctccttcgcg atgcggttga cctcggcgag gccgccggtg ctggccaggc
     1081 gaccgtaata gttgatgcgc acggcgtcgt cctggatgta ggcgggactg aggcgcgcgt
     1141 cgacggccag gtccaccgtc acctgcggca cctccgcggc gcgcagctcg cccttgagct
     1201 tggcgacctc ctccgcgagc atctccgtgt agaccgcgat cgacacggcc gagatgtggc
     1261 cgtgctgctc cggccccagc aggttgccga cgccgcggat ctccatgtcc ttctcggcca
     1321 gtaggtggcc ggagccgagg tcgttcagct ccgcgatggc gtacaggcgc cgttgcgcct
     1381 gctccgtgag cctgccgggg tagagcatgt acgcccatgc ctccgtctgg cgcctcccga
     1441 cgcgccccct cagctggtag agctgc
//
LOCUS       DAOQYE010000654         1465 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_110280_length_1465_cov_2.459574, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000654 DAOQYE010000000
VERSION     DAOQYE010000654.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1465
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110280_length_1465_cov_2.459574"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..750)
                     /locus_tag="PKN52_09860"
     CDS             complement(<1..750)
                     /locus_tag="PKN52_09860"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNR00297.1"
                     /translation="MPDRRTDDHRGSHTGERLEQLLARLRPIVSRRKGVAIVLVGPPG
                     IGKTTLAAQALTALGCRCLSLPASVGLARLVREVPSPATLPAWARLGLERAAAGEVVE
                     QTALVGAVSAALQRSAPFVLHVDDLHEAQPELVVMLEALAGACRRTPGVGLLVASRQH
                     HPQPFEELWLEPFEQADSIALLESVIGGPVPPAAAEWVYARARGNPLFTLEYQRQLAR
                     GGFLWSDGQRWNWREPQSGVRPARVEAVIEHV"
     gene            812..991
                     /locus_tag="PKN52_09865"
     CDS             812..991
                     /locus_tag="PKN52_09865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00298.1"
                     /translation="MASQCDLAGAPPLPLPEEPQLYSSNNRPPHVEQNSPNGEPIGAP
                     QLGQAFSNSAPSFGQ"
BASE COUNT          242 a          513 c          509 g          201 t
ORIGIN      
        1 cacgtgctcg atgacggcct cgacgcgagc aggtcgcact cccgactgcg gctcgcgcca
       61 gttccagcgt tgcccgtcgc tccagaggaa gccgccgcgt gccagctgcc gttggtactc
      121 gagtgtgaag agcgggttgc ctcgggcacg ggcgtagacc cactcggcgg cggccggtgg
      181 taccggacca cctatgacgg attccaggag cgcgatcgag tccgcctgtt caaagggctc
      241 gagccagagt tcctcgaacg gctggggatg gtgctgccgg ctggctacga ggaggccgac
      301 gccaggggtc cgccgacacg cgccggccag ggcctcgagc atgacaacca gctccggctg
      361 cgcctcgtgg aggtcgtcga cgtgcaggac gaagggggcg ctgcgctgca gcgcggccga
      421 gacggccccg accagggcgg tctgctcgac gacctcgcca gccgcggccc gctccaagcc
      481 gagccgagcc catgccggca gggtggccgg gctaggcacc tctcgtacca gacgcgcgag
      541 accgacgctg gccggcaggc tcaggcacct gcagccgagc gcggtcagcg cctgggccgc
      601 cagggtcgtc ttccctatcc cgggcggtcc gaccagaacg atggccacac ccttgcgacg
      661 cgagacgatg ggccgcaggc gcgccaagag ctgctccagg cgctcacctg tgtgactgcc
      721 tcgatggtca tccgtgcgac ggtctggcaa cgggtggtcc ttaagcggtc tgggccaacc
      781 ccaggctcat cgcaatgata ggccggcggc ggtggcgagt cagtgcgacc tcgcgggcgc
      841 cccgccgctc ccgctgcctg aggaacccca gctctactcg tcgaacaacc ggccaccgca
      901 cgtcgagcag aactcgccga acggcgagcc gatcggcgcc ccgcagctcg ggcaagcctt
      961 cagcaactcg gcgccgtcgt tcgggcagta gttctcaccg gccgaactcg gagtggctcg
     1021 cgagcagacg gggcagacgc ggtacgaaac gacctgcccc gccgagcccg gcggtgtcgg
     1081 cgggaccgac ggttccgagg aaccgccagt cccgaccgag gcggccgaca cgcgggcgtc
     1141 gggtgcgtcc tcggtgcggc gcgcgcttgg cgcgcgggtc ttctccgaca tgcctagatg
     1201 gtcgcccccc gtcggttatg cgccggttat agccgggtgg acctgccgtc agaacgggca
     1261 cctggtcacg acggaccaga tcgccgaacc ggcagccgcc cgccttgaca gcggccgcgc
     1321 aggcatgcga tcgtccgatc aattcatagt aatatgctat ggattggttc tgccatccgc
     1381 gcggggctag aagatcatca cacgcgaacg cttccacccg accggggaag cggcgccagg
     1441 aggaaaacat gcgcaacatc agcag
//
LOCUS       DAOQYE010000655         1464 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_110408_length_1464_cov_2.594748, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000655 DAOQYE010000000
VERSION     DAOQYE010000655.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1464
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110408_length_1464_cov_2.594748"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            231..452
                     /locus_tag="PKN52_09870"
     CDS             231..452
                     /locus_tag="PKN52_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HNR00299.1"
                     /translation="MNLNWDTVRGNWKQVKGEAKRQWGKLTDDDMLQLEGSREKLVGK
                     IQERYGLTKEAAERQVEDWARGLPEFPRA"
     gene            539..787
                     /gene="chaB"
                     /locus_tag="PKN52_09875"
     CDS             539..787
                     /gene="chaB"
                     /locus_tag="PKN52_09875"
                     /inference="COORDINATES: protein motif:HMM:NF007136.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HNR00300.1"
                     /translation="MPYDTLKELPESVRNVLPKHAQEIYQSAFNSAWEQYDKPSERRG
                     GSSREETAHAVAWAAVKKTYEKGSDDKWHKKEAVGAGR"
     gene            complement(858..1238)
                     /locus_tag="PKN52_09880"
     CDS             complement(858..1238)
                     /locus_tag="PKN52_09880"
                     /inference="COORDINATES: protein motif:HMM:TIGR02684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative addiction module antidote protein"
                     /protein_id="HNR00301.1"
                     /translation="METRSKESAPGLETFPYDVVDFLGTPEDRAEYLDVWFEESPEDT
                     RGLSKAIGDIARASGLAEVAAATGLSERGLYRSLNARRNPSFATVMKVLNALGIRLRA
                     ELVNQEGAGASNDESDAEDGSASA"
BASE COUNT          260 a          478 c          486 g          240 t
ORIGIN      
        1 ggggggctcc aacgtcggct ggaacgatgt cgagctctag gccgacccag ggtcgtctgg
       61 cgggagtgtt gccagcgcgg cgctcaaacc tgagttccgc atgagcgtcg cacagcgctt
      121 cacacgccca cctcacgcct cacaactcct ttgggcgcac actgcggtcg cgtcgccggg
      181 gcgccgccac aggggtcgcg cgttaggcgg cgatgcagga ggaggaagcc atgaacctca
      241 actgggatac cgtccgcggg aactggaagc aggtcaaggg cgaagccaag cgccagtggg
      301 gcaagctgac cgacgacgac atgctgcagc tcgagggcag ccgcgagaag ctcgtcggga
      361 agatccagga gcgctacggt ctcacgaagg aggcagcaga gcgccaggtg gaggactggg
      421 cgcgcggcct gcccgagttt cctcgcgcct gaaccagatc ggtgagccta cttaggggtg
      481 cgcgcttgcc cacaaagcgc gcaccccgca ttcgcttagc acgcaggagg tttacaccat
      541 gccgtacgac accctcaaag agttgcccga gtcggtccgc aacgttctgc cgaagcatgc
      601 gcaggagatc taccagtccg ccttcaacag cgcctgggag cagtacgaca agcccagcga
      661 gcggcgcggc gggtcctcgc gcgaggagac cgcccacgcg gtggcatggg cggccgtcaa
      721 gaagacctac gagaaggggt ctgatgacaa gtggcacaag aaggaagcgg tcggcgcagg
      781 caggtgagtg acgccggctc cgcgcgttgc ccctggtcaa tgctgcggcg caactccttc
      841 tcgctgtgcc ggttgggcta cgccgaggcg ctcccgtcct cggcgtctga ctcgtcgttg
      901 gaggctcccg cgccctcctg gttcacgagt tcggccctca ggcggatgcc gagcgcgttc
      961 aggactttca tgacggtcgc gaagcttggg ttccttcgcg cgttcaatga gcggtagagg
     1021 ccccgttcac ttaggccggt tgcggcggcg acttcagcta ggccgcttgc gcgggcgatg
     1081 tccccgatgg ccttggagag cccccgagtg tcctcagggg attcctcgaa ccagacgtcc
     1141 aggtattccg cccgatcttc cggcgtccct aggaagtcca cgacgtcgta cgggaaagtc
     1201 tcgagccctg gtgccgactc cttgctccga gtctccatgt ccacctctgc tggtgtcgac
     1261 cggccacccc ggcgctacgt gcgttctccc ggctcgaggc cgctcctcgg cgccgtcaac
     1321 gacggttgaa ctgggcgcac ccagcgaatg tggctcgtaa gctacgacaa tgccgctcgg
     1381 gcgtccgtga ggatcgagcg aacacaagta cacgagtgac gacgccactc acatggcgcc
     1441 atccctggtc aagcgcctag ttcg
//
LOCUS       DAOQYE010000656         1463 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_110545_length_1463_cov_2.346591, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000656 DAOQYE010000000
VERSION     DAOQYE010000656.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1463
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110545_length_1463_cov_2.346591"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="PKN52_09885"
     CDS             <1..371
                     /locus_tag="PKN52_09885"
                     /inference="COORDINATES: protein motif:HMM:NF023431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3500 domain-containing protein"
                     /protein_id="HNR00302.1"
                     /translation="TDVLLGPDADGGFPQARSGIAVSTLSDDQKALVLAAMKPWVSIV
                     DEQAAEDLLAIYEAELDDTYISYSGATALTSQGDYIRIDGPSVWIEFVCQNGVVFSDQ
                     IHYHTVYRDRVRDYGGELSF"
     gene            374..>1463
                     /locus_tag="PKN52_09890"
     CDS             374..>1463
                     /locus_tag="PKN52_09890"
                     /inference="COORDINATES: protein motif:HMM:NF025167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HNR00303.1"
                     /translation="MRTRPAIHTLWITLLAAGALSFMAHARAHPMPHSVVALTVGADQ
                     VSARVELPLDDLVLALGSDPLSDSLEDVGQRVAAYLADHVTAVSPDGTAWRTTITSVA
                     ASETEQTDAGVYQELVAQLTLTPPSGESTRRFIFDYDAIIHQVITHKILVTVESDWAG
                     GNLGDVYEVGVIRVDTGTGQISPLAVDLGNGSGWTGFTAMVGLGVSHILEGTDHLLFL
                     LVLLLPAPLMALPRRWGGPIPSRRAVGRILAITAAFTIGHSATLILAALTRLELPAGP
                     IEALIAVSILVGAVHALRPLFPGRETLIAAGFGLLHGMAFSFTLRELDLSTNQLVLSL
                     LGFNLGIEAMQLLVVAAVLPALLLASRSG"
BASE COUNT          226 a          510 c          458 g          269 t
ORIGIN      
        1 tcactgacgt gctgctcggc ccggacgccg acggcggttt cccgcaggcc cgcagcggga
       61 tagcggtctc cacgctgtcc gacgatcaga aggcgctcgt gttggcggcc atgaagccgt
      121 gggtgtcgat cgtcgacgag caggcagccg aggacctgct cgccatctac gaggcggaac
      181 tcgacgacac gtacatctcc tactccggcg ccacggcgtt gacgtcgcaa ggcgactaca
      241 tccgcatcga tggaccgagc gtgtggatcg agttcgtctg ccagaacggc gtcgtcttct
      301 ccgatcagat ccactatcac acggtctacc gcgaccgcgt gcgcgactac ggtggcgagc
      361 tgtcgttcta gccatgagga cgcgtcctgc catccatacc ttgtggatca ctctgctcgc
      421 ggctggtgcc ctgtcgttca tggcccacgc ccgagctcac ccgatgcctc actcggtcgt
      481 tgccctgacc gttggcgccg atcaggtgag tgcgcgcgtc gaactcccgc tggacgacct
      541 agtgctggcg ctgggttcgg accccctgag cgacagcctg gaggacgtcg gccagcgggt
      601 ggcggcttac ctggccgacc acgtcaccgc cgtctcgccc gacggcaccg cttggcgcac
      661 gacgatcacg agcgtcgcag cgagcgagac ggagcagacc gatgccggcg tgtaccagga
      721 actcgtggcg cagctgacgc tcaccccgcc gagcggagag tccaccaggc ggttcatctt
      781 cgactacgac gccatcatcc atcaggtgat cacgcataag attcttgtca ccgtcgaaag
      841 cgattgggcc ggcggcaacc tcggcgatgt gtacgaggta ggggtgatcc gcgtcgacac
      901 cggcacgggg cagatctctc cgctggcggt cgacctcggg aacggcagcg gctggacggg
      961 cttcacggcg atggtcggac tcggcgtgtc gcacatcctc gagggcaccg accacctact
     1021 gttcctgttg gtgctcctct tgccggcgcc tctgatggcg ctgcctcgcc gctggggtgg
     1081 accgatcccg tctcgccggg ccgtcggacg catcctggcc atcacggccg ccttcaccat
     1141 cggacactcc gcgacgctca tcctcgcggc acttacccga ctcgagctgc cagcaggacc
     1201 gatagaggct ctgatcgccg tgagcatcct ggttggtgcc gttcacgcct tgcgccccct
     1261 cttcccagga cgcgagaccc tgatcgcggc cggcttcggc ctactgcatg gcatggcctt
     1321 ctcgttcacc cttcgcgaac tcgacctgag caccaaccaa ctcgtgctga gcctgctagg
     1381 gttcaacctc gggatcgagg cgatgcaact gctcgtcgtg gccgccgtgt tgccggcgct
     1441 gctcctcgct tcgcgctctg gag
//
LOCUS       DAOQYE010000657         1462 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_110631_length_1462_cov_2.810945, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000657 DAOQYE010000000
VERSION     DAOQYE010000657.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1462
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110631_length_1462_cov_2.810945"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1010)
                     /locus_tag="PKN52_09895"
     CDS             complement(<1..1010)
                     /locus_tag="PKN52_09895"
                     /inference="COORDINATES: protein motif:HMM:NF024941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4127 family protein"
                     /protein_id="HNR00304.1"
                     /translation="MKGPTTIDSLLLIPLDERPINAAYPRLLTDALGWNLACPTALLG
                     GRKTPADVAGLADWLLAAAGGADPSGRPLGAVIALDTLAWGGLIPSRQSGNDLGAALE
                     RLAVLRRVRELAPSLPILAFSSIQRVSREDDDAEEPSYYRSHGRAIFRRSVLEHRAVA
                     AELTNDEERELAGLRVGVPADVWQDQLDIRRRTLAVNLAALDLVADGVIDTLVLNQDD
                     TAEWGLNVMNRRRFEAEVRRRDLEDRVLVYPGADEVAQALIARLASIVLGRRARVSAF
                     YASRLGAGVRTAYEDRPLGDLVTVHLRAAGAVQAPPGTPVDLWLGVNSPSRSQGQGGV
                     SHA"
     gene            complement(1031..>1462)
                     /locus_tag="PKN52_09900"
     CDS             complement(1031..>1462)
                     /locus_tag="PKN52_09900"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNR00305.1"
                     /translation="GWVGTFLPLIVPALTGSAFAVFLLRQFYSAIPNDISDSARIDGA
                     SEWQIFARIILPLAKPALATASLFIFIWTWTDFLNPLIYLTDDRLYTLAIGLQQLAST
                     RAAAWPLLMAGSLLMSVPIILLFFFAQKTFIAGVSTAAVKG"
BASE COUNT          226 a          472 c          569 g          195 t
ORIGIN      
        1 gcgtgcgaca cgcccccctg gccttgcgac cgcgaggggc tgttgacgcc cagccagagg
       61 tcgacgggcg tgccgggtgg cgcctgcacg gcgcccgcag cgcggaggtg aaccgtgacg
      121 agatcgccga ggggtcggtc ctcgtacgcc gtccgcacgc cagcgccaag gcgcgacgca
      181 tagaacgcgc tcacgcgcgc ccggcgcccg aggacgatgg aggcgaggcg cgcgatgagc
      241 gcctgggcga cctcgtcggc gccggggtag acgagcacgc ggtcctccaa gtcgcggcgg
      301 cgcacctccg cctcgaaccg gcgccggttc atgacgttga ggccccactc cgccgtgtcg
      361 tcctggttga gcacgagcgt gtcgatgacg ccgtccgcca cgaggtcgag cgccgccagg
      421 ttgaccgcca gcgtgcgccg cctgatgtca agctggtcct gccagacgtc ggccggcaca
      481 cccacgcgta ggccggccag ttcgcgctcc tcgtcgttgg tcaactccgc ggcgacggcg
      541 cggtgctcca gcacggagcg ccggaagatg gcgcgcccat gcgagcggta gtagctcggc
      601 tcctccgcgt cgtcgtcctc gcggctgacg cgctggatgc tgctgaaagc gaggatgggc
      661 aggctcgggg cgagttcccg cacgcgccgc aacacggcga ggcgctcgag ggcggcgccg
      721 aggtcgttgc cggactgccg cgaggggatg aggccgcccc atgcgagggt gtcgagcgcg
      781 atgacggcgc ctaggggccg tccggagggg tcggcgcccc cggcggcggc gagcagccag
      841 tcggccagtc cggccacgtc ggcgggcgtc ttgcgcccgc ccagcaaggc cgtcgggcac
      901 gcaaggttcc aaccgagagc gtccgtaagc aggcgcgggt aggcggcgtt tatggggcgc
      961 tcgtcgagcg ggatgagtag gagcgagtcg atggtcgtag gtcccttcat gggcgttggc
     1021 gcctgggcgg tcaacccttc acggccgctg tcgagacgcc cgctatgaac gtcttctggg
     1081 cgaagaagaa gaggaggatg atgggcacgc tcatgaggag cgagccggcc atcaggagcg
     1141 gccaggcggc ggcgcgggtg ctcgcgagct gctgcaggcc gatggcgagc gtgtagaggc
     1201 ggtcgtcggt cagatagatg agcgggttga ggaaatccgt ccaggtccag atgaatatga
     1261 acagcgacgc cgttgccagc gcgggcttgg cgagggggag gatgatgcgc gcgaagatct
     1321 gccactcgct cgccccgtcg atccgcgccg agtcggagat gtcgttcggg atggccgagt
     1381 agaactggcg cagcaggaac accgcgaagg cgctgcccgt gagcgccggc acgatgagcg
     1441 gcaggaaggt gccgacccag cc
//
LOCUS       DAOQYE010000658         1462 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_110669_length_1462_cov_2.181947, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000658 DAOQYE010000000
VERSION     DAOQYE010000658.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1462
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110669_length_1462_cov_2.181947"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            200..>1462
                     /locus_tag="PKN52_09905"
     CDS             200..>1462
                     /locus_tag="PKN52_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HNR00306.1"
                     /translation="MREIRNFGRIKEVIELPNLTNIQITSYNNLLQLGKSAAERDDTV
                     GLQAAFREVFPIDETERGRQTDLVIDFLEYRLDPPEYGPDECREKDLTYHAGLFAKLQ
                     LVHKDTGLIKEDEVFLGDLPLMTDDGSFIINGADRVIVSQIHRSPGVYFTESIVGVNQ
                     KRYTASIIPMPKRGPWIEVEFDNSGVLWMKVNKRKFQFTFLLRVLGYSDVQIRELFAG
                     HESQIEPTLESEGAAGITPDEALLRLFTVLRPGDPPKVDKATQYLFGLLADPRRYDLG
                     AAGRFKMNEKLKLDYPSNTLLGFQDGRFVDYGLVPTLQYLVRLQAGEEGYQVDDIDDL
                     GNRRIRTVGELVADQIRVGLGRMARGVRERMLLGNPDAATPQKLVNSRPIVAALREFF
                     GRSQLSQFKDQVNPLSELRHKRRISALGP"
BASE COUNT          271 a          552 c          429 g          210 t
ORIGIN      
        1 atgctctcgc cacctcccgc gccagacggg cgcgggcccg cacaggacca gtgcggggtt
       61 cggggggcct gaagcgcata ttaaccacat tcggaccaca ccaggcaacg cccgagtcct
      121 cggctgagcg cgagacgcgc gccggccgcg gcacctgggc gccgccacgc cagactcggc
      181 ttctagcgaa gggatgaaga tgcgcgagat ccggaacttc ggcaggatca aggaagtcat
      241 cgagcttccg aacctgacca acatccagat cacctcgtac aacaacctgc tccaactcgg
      301 gaagagcgcc gcagagcgcg acgacaccgt tggcctgcag gctgctttcc gcgaggtgtt
      361 cccgatagac gagaccgaac gcggtcgcca gaccgacctc gtcatcgatt tcctagagta
      421 ccgcctcgac ccgccggagt acggcccgga cgagtgccgc gagaaggacc tcacctacca
      481 cgccggcctg ttcgccaagc tccaactcgt ccacaaggac acgggcctca tcaaggaaga
      541 cgaggtcttc ctcggcgacc tgcccctcat gacggacgac ggctcgttca tcatcaacgg
      601 tgccgaccgc gtcatcgtct cccagatcca ccgcagcccc ggcgtctact tcaccgagtc
      661 gatcgtgggc gtcaaccaga agcgctacac ggcgagcatc atccccatgc cgaagcgcgg
      721 cccgtggatc gaggtcgagt tcgacaactc cggcgtgctg tggatgaagg tcaacaagcg
      781 caagttccag ttcaccttcc tcctgcgcgt cctcggctat tccgacgtcc agatccgcga
      841 gctgttcgcc ggccacgagt cccagatcga gccgacgctc gagagcgagg gggccgccgg
      901 catcaccccg gacgaggccc tgctccggct cttcacggtc ctgcgccccg gcgacccgcc
      961 caaggtcgac aaggcgaccc agtacctctt cgggctcctc gcggaccccc gccgctacga
     1021 cctcggcgcg gccgggcgct tcaagatgaa cgagaagctc aagctcgact acccgtccaa
     1081 cacgctcctc gggttccagg acggtcgctt cgtcgactac ggactcgtgc cgaccctcca
     1141 gtacctcgtg cgcctgcagg ccggcgaaga gggctaccag gtcgacgaca tcgacgacct
     1201 cggcaaccgc cgcatccgca ccgtaggcga gctcgtcgcc gaccagatcc gcgtcggcct
     1261 cgggcgcatg gcgcgcggcg tgcgcgagcg catgctgctc ggcaaccccg acgccgccac
     1321 gccccagaag ctcgtcaaca gccggccgat cgtcgccgcg ctgcgcgagt tcttcggccg
     1381 cagccagctc agccagttca aggaccaggt caacccgctc tccgagctcc gtcacaagcg
     1441 ccgcatctcc gccctcggcc cg
//
LOCUS       DAOQYE010000659         1460 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_110872_length_1460_cov_2.817082, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000659 DAOQYE010000000
VERSION     DAOQYE010000659.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1460
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110872_length_1460_cov_2.817082"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1096
                     /locus_tag="PKN52_09910"
     CDS             <1..1096
                     /locus_tag="PKN52_09910"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNR00307.1"
                     /translation="REHNRLAVLEALLARRVAARSELSRATRLSVPTVTTIGQELVAL
                     GIVDEGGVGPSGGGRPARLLRLVPSARNVLSVDLSGGRVRAARIDLSGELFELEPGPS
                     LAPGLEGELAGWLRAAVAEAAAAGTPYAWLAVAIPGVIGQDGSSVRLAPSLGWHEFDA
                     GDLLRKASGLRVLLENDVNALAIGELHHGVGQGHKDVVYLTITGGVGAGIVLGGQLYR
                     GSHRAAGEVGYSLLPGLPEEGLELGAPGPLERHLLSIVADVVVNGELDLEAPERAAAF
                     DRLAAGVRLVLHNLACVFDPELVVVAWAADPEGRLADELARRWSLPQATSIRAGSLGP
                     IAALHGLGQLALERLKSEVASLEQHVGSQS"
     gene            1093..>1460
                     /locus_tag="PKN52_09915"
     CDS             1093..>1460
                     /locus_tag="PKN52_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00308.1"
                     /translation="MSYRHLRVIDMHVHFPIANTLGLPPDPKQQHPLLQAYARERGER
                     MAREWNTEPTEPAARTDAEVDAYLARWVGEVERYGLRKVVFVSANTNDRLADIVARHP
                     DKFIGFAHHALRPGAAAELRR"
BASE COUNT          199 a          570 c          485 g          206 t
ORIGIN      
        1 gcgcgagcac aaccgcctgg cggtgctcga agcgctcctc gcgcgccggg tggccgcgcg
       61 ctccgaactc tcccgcgcca cccgtctcag cgtccccacc gtcaccacca tcggccagga
      121 actcgtggcg ctcggcatcg tcgacgaggg cggggtcggt ccttccggcg gcgggcgccc
      181 ggcgcggctc ctacgcctgg tgccttcggc acggaacgtc ctgtccgtcg acctctccgg
      241 cggtcgcgtg cgcgccgcgc gcatcgacct gtccggcgag ctcttcgagc tcgagcccgg
      301 cccaagcctc gcccccggcc tggaaggcga actcgccggc tggctccgcg ccgccgtcgc
      361 ggaggccgcc gccgccggca ccccgtacgc ctggttggcc gtcgccatcc ccggcgtcat
      421 cggtcaggac ggctctagcg ttcgcctcgc gcctagcctc ggctggcacg agttcgacgc
      481 cggcgacctc ctccgcaagg ccagcgggct gcgcgtgctg ctcgagaacg acgtcaacgc
      541 actcgccatc ggcgagctgc accatggcgt cggtcagggc cacaaggacg tcgtctacct
      601 caccatcact ggcggcgtcg gcgccggcat cgtgcttggc ggccagctct accgcggttc
      661 ccaccgggcg gcgggggaag tcggctacag cctcctcccc ggcctgcccg aagagggtct
      721 cgaactcggc gcaccaggcc cgctcgagag gcacctgctc agcatcgtcg ccgacgtcgt
      781 cgtgaacggc gaactcgatc tggaggcgcc tgagcgcgcc gccgccttcg accgccttgc
      841 cgcgggcgtg aggctcgtcc tacacaacct ggcctgcgtc ttcgaccccg aactcgtcgt
      901 ggtggcgtgg gccgcggatc ccgaaggtcg cctggcagac gagctcgcgc gccggtggtc
      961 gttgccccag gccacctcga taagggccgg ttcgctcgga ccgatcgcgg cgctgcacgg
     1021 tctgggccag ctcgccctcg agcgcctgaa gtccgaggtc gcgagcctag agcagcacgt
     1081 tgggagccag tcatgagcta ccgtcacctc cgcgtcatcg acatgcacgt gcacttcccc
     1141 atcgccaaca cgctgggcct gccgccagac cccaagcagc agcacccgct cctacaggcg
     1201 tacgcgcggg agcgcgggga gcgcatggcg cgcgagtgga acactgaacc gaccgagccg
     1261 gccgcgcgca ccgacgcgga ggtcgacgcc tacctagcgc gctgggtcgg cgaggtggag
     1321 cgctacggcc tgcgcaaggt cgtgttcgtg agcgccaaca ccaacgaccg cctcgctgac
     1381 atcgtggccc gtcaccccga caagttcatc ggcttcgccc accacgccct gcggcccggc
     1441 gccgccgccg agttgagacg
//
LOCUS       DAOQYE010000660         1460 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_110906_length_1460_cov_2.281851, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000660 DAOQYE010000000
VERSION     DAOQYE010000660.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1460
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110906_length_1460_cov_2.281851"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="PKN52_09920"
     CDS             <1..385
                     /locus_tag="PKN52_09920"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178595.1"
                     /note="E3 component of pyruvate and 2-oxoglutarate
                     dehydrogenase complex; catalyzes the oxidation of
                     dihydrolipoamide to lipoamide; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HNR00309.1"
                     /translation="TVVPSVIYTEPELASVGMTEAEAKEAGFEVRVGRFPLSASGRAS
                     TLGVSGGLVKLIGDAKTDILLGFHMVGPNAGDIVSEAALAIEMGATLEDIALTQHPHP
                     TISEGVMEAAEHAHGKAIHIANRRR"
     gene            complement(488..>1460)
                     /gene="argJ"
                     /locus_tag="PKN52_09925"
     CDS             complement(488..>1460)
                     /gene="argJ"
                     /locus_tag="PKN52_09925"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HNR00310.1"
                     /translation="KPVRAVIVNSGNANCATGEQGVRDDLAFAAAAAVALGLAGPDEV
                     VSARTGVIGHKLPLDPLVAAMPQLAAERSESVEGFARAILTTDLTEKVAAAEVRGGAR
                     VLGVAKGSGMIHPNMATMFGFLMTDALIDQATLRAAWQRVVDRTFNQLTVDGDTSTND
                     MAVVLASNLVPADAYELELALTEVATELAKHIARDGEGATKLITVRVRGALTDVEARR
                     AARTVAGSSLVKTAVHGADPNWGRILAAAGRADVALYMARAAVFAQGTALYRGAPLAY
                     DARAVSHALREPDVLLEVDLGVGSAEGTAWGCDLTEGYVRINADYTT"
BASE COUNT          209 a          533 c          494 g          224 t
ORIGIN      
        1 cacggtcgtg cccagcgtca tctacaccga gcccgagctg gccagcgtgg gcatgaccga
       61 ggcggaggcc aaggaggccg gcttcgaggt gcgcgtcggg cgcttcccgc tctccgcctc
      121 gggtcgggcg tcgaccctcg gcgtgtcagg tgggctcgtg aagctcatcg gggacgccaa
      181 gaccgacatc ctgctcggct tccacatggt cggccccaac gcgggcgaca tcgtgtcgga
      241 agcggcgctc gccatcgaga tgggtgccac gctcgaggac atcgccctca cccagcaccc
      301 gcacccgacg atctcggaag gggtcatgga ggcggccgag cacgcgcacg gcaaggccat
      361 ccacatagcc aaccgcaggc gctgatcggg cgccgcgccc cgacgcgacg atgcccagac
      421 agcgcgttgt gccggtctgg cgaccggcac aacgcttctc agggttcggg ctaccgcgga
      481 ccgggagtca ggtcgtgtag tccgcgttga tgcggacgta gccctccgtc aggtcgcagc
      541 cccaagccgt gccttcggca gaaccgactc cgagatccac ttccaatagc acgtcgggct
      601 cccgaagcgc gtgcgacact gcgcgcgcgt cgtaggctag tggcgccccc cgatagagcg
      661 ccgttccctg ggcgaacacc gccgcgcgcg ccatgtagag cgctacgtcc gcccgcccgg
      721 ccgcggccag gatgcgcccc cagttcgggt cggcgccgtg cacggccgtc ttgaccaggc
      781 tgctccccgc caccgtgcgg gccgcacgtc gcgcctccac gtcggtcagc gcgccccgca
      841 cgcgtaccgt gatgagcttg gtggcgccct cgccgtcgcg cgcgatgtgc ttggccagtt
      901 cggtggccac ttccgtcaac gcgagctcaa gctcgtacgc gtcggcaggg accaggttcg
      961 acgcgagtac taccgccatg tcgttggtcg aagtgtcgcc gtcgaccgtc aactggttga
     1021 aggtccggtc cacgaccctt tgccacgcgg cgcgcagcgt cgcctggtct atcagggcat
     1081 ccgtcatcag gaacccgaac atggtggcca tgttcgggtg gatcatcccg gagcccttcg
     1141 cgacgccgag cacgcgcgcc ccgcccctca cctcggcggc cgccaccttc tcggtgaggt
     1201 ccgtcgtcag gatggcgcgc gcgaagcctt cgacgctctc ggagcgctcc gcggccagtt
     1261 gcggcatggc cgcgacgagc ggatctaggg gtagcttgtg gccgatgaca cccgttctgg
     1321 cgctgacgac ctcgtcgggg ccggccaggc caagggcgac ggccgctgcc gcggcgaacg
     1381 ccagatcgtc gcgcacgccc tgctcgccgg tggcgcagtt cgcgttgccg ctgttcacga
     1441 tcacggcgcg caccggtttg
//
LOCUS       DAOQYE010000661         1459 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_111021_length_1459_cov_2.348291, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000661 DAOQYE010000000
VERSION     DAOQYE010000661.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1459
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111021_length_1459_cov_2.348291"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            61..>1459
                     /locus_tag="PKN52_09930"
     CDS             61..>1459
                     /locus_tag="PKN52_09930"
                     /inference="COORDINATES: protein motif:HMM:NF017539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NACHT domain-containing protein"
                     /protein_id="HNR00311.1"
                     /translation="MAKLTTLGGLALHPGPFTQPKPLAMLAYLALEGRKPRRHLAELL
                     WPDGNGLKSLSMAITRLRKADQLACVADTRLVWSELECDATRLLTAIEADDLAAALTL
                     YEGAFLEGATFQGASAELEEWVLETRERLAEGFRALLIRHAETALDTGKPEHAAELAA
                     SAYRVAGAAPASPESLQRLHRLFALSHHELANAVMAELEELGADRAPGQESPLARQAR
                     ALFSTPFVGREAELAAVVGALGRRETRVLTLLGPAGSGKTRLAAQAALQAEEHGPFAG
                     AVRLALLDDVDQPQLLPSRLSQVLGFQTPPSDDPWQQLAGAIGDRKLLLVLDNMEQLP
                     RVADDLGRLVTAAPGLTLLLTSRALLGVPGEEALAVGGLPAPEAGTAWDEALRYPAVR
                     LLNDLALALKPNLDLRSQAAGVLRVCQELGGLPLGLELAAAWLRILPAAALAERLASD
                     PASLDGAGPRAQGDDG"
BASE COUNT          216 a          525 c          515 g          203 t
ORIGIN      
        1 gccacgatca cacggcgacc gagccgacgg cactagactt acgcctacgg gagttggcgc
       61 atggcgaagc tgaccacgct cggcggtttg gcgctacatc ctggcccctt cacgcaaccg
      121 aaaccgctgg ccatgctcgc ctacctggcc ttggagggca ggaagccacg ccgtcacctg
      181 gcagagctgt tgtggccgga cggcaacggg ctgaagagcc tctcgatggc catcacgcgt
      241 ctgcgcaagg ccgaccaatt ggcctgcgta gcagacacga ggctcgtctg gagcgagctg
      301 gaatgcgacg ccacgcgctt gttgacagcc atcgaagccg acgacctcgc tgccgcgttg
      361 accctttacg aaggggcctt cctcgagggg gcgacgttcc agggtgccag cgccgaactc
      421 gaggagtggg tacttgagac ccgcgaacgc ttggccgagg gcttccgcgc gctcctcata
      481 cgccacgccg agacggcgtt ggacaccggg aagccagagc acgccgcaga gctggccgcc
      541 agcgcctacc gggtggccgg agcggcgccg gccagcccgg agagcctgca gcggctccac
      601 cgcctgttcg cgctgagcca ccacgagctg gcgaacgcgg tcatggcgga actcgaggag
      661 ctgggcgccg accgggcccc gggccaggag tcgccgctgg cccggcaggc gcgcgcgctg
      721 ttctccaccc cgttcgtcgg ccgcgaggcg gagcttgccg ccgtcgtggg cgctttgggg
      781 aggcgggaga cgcgggtcct cactctcctt ggcccggccg ggagcggcaa gacgcgtctg
      841 gcagcgcaag ctgccctaca agccgaggag cacggtccgt tcgccggcgc cgtccgcctg
      901 gcgctcctcg acgacgtgga ccagccgcag ttgctccctt cgcgcctcag ccaagtgctg
      961 ggtttccaga ctcctccaag tgacgacccg tggcagcaac tggcgggcgc catcggcgac
     1021 cggaagcttc tgctcgtact ggacaacatg gagcagttac caagggtggc ggacgacctc
     1081 ggccgcctcg tgaccgccgc gccgggcctc accctgctgc tgacgagccg ggcgctgctc
     1141 ggcgtgccgg gtgaggaggc gctggccgtc gggggcctac ccgccccgga ggcagggacc
     1201 gcctgggacg aggcgctacg ctacccggcc gtccggctgc tgaacgacct cgctcttgcc
     1261 ctgaaaccga acctcgacct gcggtcccag gccgccggcg tgctgcgcgt ctgccaagag
     1321 ctcggcggcc tgcccctcgg actggagctg gcggcggcct ggctccgcat cctgcccgcc
     1381 gccgcgctgg cggagcggct cgcctccgac cccgcctcgc tcgatggggc aggaccgaga
     1441 gcgcagggtg acgacggtg
//
LOCUS       DAOQYE010000662         1458 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_111129_length_1458_cov_2.576622, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000662 DAOQYE010000000
VERSION     DAOQYE010000662.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1458
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111129_length_1458_cov_2.576622"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="PKN52_09935"
     CDS             complement(<1..246)
                     /locus_tag="PKN52_09935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNR00312.1"
                     /translation="MKKLALLLGLLLTLGLTSAQTLVFGSSGFPASLDAVDSQDGNSL
                     VVSGQVTERLVDFAPGKTDLIPGLAASWSANDDATVWT"
     gene            complement(403..1101)
                     /locus_tag="PKN52_09940"
     CDS             complement(403..1101)
                     /locus_tag="PKN52_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNR00313.1"
                     /translation="MTPILASRDLERRFDSPGGAIEVLSDATLTLGAGEVVAILGPSG
                     SGKSTLLHLLGGLDRPDGGEIYWGDFPVHEHAPRQLAARRARYVGLVFQNHYLLQDFT
                     LLENVAMPSRILGAPDEQRAAALLERVGLAGRLHHSPSKVSGGERQRAAVARALALRP
                     KVILADEPTGSLDHVRAEEVFDLLVALAAEEGTAVVAVTHDERLVDRPGVRKLRLVDG
                     RLVPAEVTAGEVRG"
     gene            1189..>1458
                     /locus_tag="PKN52_09945"
     CDS             1189..>1458
                     /locus_tag="PKN52_09945"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNR00314.1"
                     /translation="MNSDAERLEILELLRRVPLFIGAPDRALDIAAAAVRKRAYEAGA
                     TMFQEGDKGEALYIIASGLVKLSKVDLGGHEKTLAILQCPEYFGEM"
BASE COUNT          230 a          501 c          501 g          226 t
ORIGIN      
        1 cgtccagacg gtggcgtcgt cgttggcgct ccacgaggcg gccaggccgg ggatgaggtc
       61 ggtcttgccg ggcgcgaagt cgacgaggcg ctccgtgacc tgtccgctga cgacgaggga
      121 gttgccgtct tgcgaatcga ccgcgtcaag cgaggcgggg aagccgctcg acccgaacac
      181 gagggtctgt gcgctcgtca acccgagcgt gaggagtaga ccgagcagca gtgccagttt
      241 cttcatgtag cctccagttt ccacgacgtg gtaggcatgg ggctgcctcg gcgctttcgt
      301 atgggtgacg cgctcgcgct gcccttgctg taccaacgtt cgacgcctgc gatgctagca
      361 cgtgaagtac cgcacacgag gagtccgtgc ttcaggttcc gttcacccgc gcacctcccc
      421 cgccgtgacc tcggcgggga ccagccgccc atcgacgagc ctgagtttgc gcacgccggg
      481 ccggtcgacc aggcgctcgt cgtgcgtcac ggctaccacg gccgtgccct cctcggccgc
      541 caacgcgacg agcaggtcga acacctcctc cgcccggacg tggtcgaggg agccggtcgg
      601 ctcgtcggcc aggatcacct tgggccgcaa ggcgagcgcg cgcgccacgg ccgccctctg
      661 ccgctcgccg cccgatacct tcgacggcga gtggtgaagg cgcccggcca agcccacgcg
      721 ctccagcagc gcggcggcgc gctgctcgtc cggcgcgccc agtatgcgcg atggcatggc
      781 gacgttctcg agcagcgtga agtcctgcag caggtagtgg ttctggaaca cgagcccgac
      841 gtaacgggcc ctgcgggcgg cgagttgcct cggagcgtgc tcatggaccg ggaagtcgcc
      901 ccagtagatc tcgccgccgt ccggtcggtc gagcccaccg aggaggtgga gcagcgtgct
      961 cttccccgag ccggacggac cgaggatggc gacgacctcg cccgccccca acgtgagcgt
     1021 cgcgtccgag aggacctcga tcgctccgcc cggcgagtcg aaccgacgtt ccaggtcgcg
     1081 actcgcaagg atgggcgtca cagggttact atacgggccg gtcgcgagaa cgttgctcca
     1141 caccgcgccc cggctatccc cgggccgtca agccacttgg agttctttgt gaacagcgat
     1201 gcggaacgac tcgagatcct ggaactgctg aggcgggtcc cactcttcat cggggcgccc
     1261 gacagggcgc tcgacatcgc cgcggcggcc gtgcgcaaac gggcctatga ggccggcgcc
     1321 acgatgttcc aggagggcga caagggcgag gccctctaca tcatcgcctc cgggctggtg
     1381 aagctctcca aggtcgacct cggtggtcac gagaagacgc tcgccatcct gcagtgcccg
     1441 gagtacttcg gcgagatg
//
LOCUS       DAOQYE010000663         1458 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_111177_length_1458_cov_1.925873, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000663 DAOQYE010000000
VERSION     DAOQYE010000663.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1458
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111177_length_1458_cov_1.925873"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1437)
                     /locus_tag="PKN52_09950"
     CDS             complement(<1..1437)
                     /locus_tag="PKN52_09950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00315.1"
                     /translation="MAGADLVERRGVRAWRLGGRLLGVVLVLVLASCQTAPPPPAPAA
                     DPDFDQAAELEALNLPVNESLRVRFLPPIAKKNPTGSFVSGLDLEIKFFNVNPVTGVA
                     SGSALSGTLSTAKRTIVSQRDTYATIWLALGTTLDRKATQYLRAEIRTAGAGNAPVCN
                     GTSQDCLGYLDIFAFKGLFLGRQNVPDGFVPVPSLLGVLPIGFKVLAAPVVPEEPVGG
                     PPPATLGELTGLSGGTLDEQAGNCASSFLTRPGQGLQAVGAGLQAVGAVGGLFQGQTS
                     SFNGTLVTPAQIGDELYGQFLNTGNSKRSVIFVVDDFSNGFELPAAALQPGTDLATLT
                     GQISHGTLVLHHVLEILASMDHGTGNDVYQISQGAFDGQPYYEYSYLGTEFFVQAVNV
                     GQFDTDTIADRLREAMLHYGEVEGASIMVVNMSFAIVPCSVLSDYSSTSGLATFEAYL
                     GALAAHNHVGSEYLDQLDELVSTPVGLGS"
BASE COUNT          263 a          466 c          502 g          227 t
ORIGIN      
        1 gctcccgagt ccgacgggag tggagacgag ctcgtccagc tggtcaaggt actcggaccc
       61 gacgtgattg tgcgccgcca gcgccccgag gtacgcctcg aaggtcgcga gcccggacgt
      121 cgaggagtaa tcgctcagaa cgctgcaggg cacgatggcg aagctcatgt tcacgaccat
      181 gatgctcgcg ccctccactt cgccgtagtg aagcatggcc tcccggagcc tgtcggcgat
      241 cgtgtcggtg tcgaactgac caacgttaac ggcctggacg aagaactccg ttcccaggta
      301 gctgtactcg taataaggct ggccgtcgaa ggcgccttgg gatatctggt acacgtcgtt
      361 gccagtgccg tgatccatgg acgccaggat ctcgagaacg tgatggagaa cgagggtacc
      421 gtgcgagatc tgccccgtca gggtggcgag gtccgtcccg ggttgcagcg cggcagccgg
      481 cagctcgaag ccgttgctga aatcgtccac gacgaagatg acggaccgct tgctgttccc
      541 cgtgttgagg aactgcccgt acagctcatc gcctatctgc gccggcgtga cgagcgtgcc
      601 gttgaagctg ctcgtctgtc cctggaacag ccctccgacg gcgccgacgg cctggagccc
      661 agctccaacg gcctgcaggc cctgccccgg ccgcgtgagg aacgaggaag cgcagttacc
      721 ggcctgctcg tcgagcgtgc cgcccgataa gccggtcagc tcgccgagcg tcgccggcgg
      781 cggcccgcca acgggctcct ccggcactac cggcgccgcg agcaccttga acccgatagg
      841 cagcaccccg agtagcgacg gaacgggcac gaagccgtcc ggcacgttct gccggccgag
      901 gaagaggccc ttgaacgcga agatgtcgag gtaaccgagg cagtcctgcg acgtcccgtt
      961 acacacgggc gcgttaccgg cgccggccgt ccggatctcg gcgcgcaggt actgggtcgc
     1021 cttgcgatcg agcgtggtcc cgagggcgag ccagatggtg gcgtaggtgt cacgttggga
     1081 gacgatcgtg cgcttggcgg tggacaacgt gccgctgagg gccgagccgc ttgcgacgcc
     1141 ggtgaccggg ttgacgttga agaacttgat ctccaggtcg agaccgctga cgaagctgcc
     1201 cgtcgggttc ttcttggcga tcgggggaag gaagcgcacg cgcaaggact cgttcacggg
     1261 aaggttcagg gcctctagtt cggcggcctg gtcgaagtcc gggtcggcag ccggggcggg
     1321 aggaggtgga gcggtttggc acgaggcaag gactagcacc aacaccacgc cgagcaggcg
     1381 tccgccaaga cgccacgccc ggacaccacg tcgctccacg agatccgcac cagccatcaa
     1441 gagccctcca gccgacag
//
LOCUS       DAOQYE010000664         1457 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_111251_length_1457_cov_2.582026, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000664 DAOQYE010000000
VERSION     DAOQYE010000664.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1457
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111251_length_1457_cov_2.582026"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="PKN52_09955"
     CDS             complement(<1..209)
                     /locus_tag="PKN52_09955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00316.1"
                     /translation="MTNSFTSSTRRRPGWRRPTLLILAALIWGIASAQTYHEAPALAD
                     LVAKGQLPPVEERLPKNPLVVNVVD"
     gene            759..>1457
                     /locus_tag="PKN52_09960"
     CDS             759..>1457
                     /locus_tag="PKN52_09960"
                     /inference="COORDINATES: protein motif:HMM:NF024376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HNR00317.1"
                     /translation="MRPLLIATALGALILAACSPPQPDPTPDPLVFGVPSVLTANPGQ
                     AAQFAAELERRLDRPVTVQTVDRAVDLTQSLEDGTFDFAHLPPSMTAQTIAALELPLV
                     AIVSRGGGTTYRAQYNARCDLGLTGLGDLAGLRFGFVSNRSASGHLVPYLELTDAGID
                     PEVDLTTSFAGSHDAVILGIYANDLDAGVSWEDARVAVSGDHPDVNDVVCVIGYSTPL
                     PNDAVLARAGLGADV"
BASE COUNT          221 a          511 c          492 g          233 t
ORIGIN      
        1 tcgtcgacga cgttgacgac cagcgggttc ttgggcaggc gttcttcgac gggcgggagc
       61 tggcccttgg cgacgaggtc ggccagggcg ggggcctcgt ggtacgtctg ggcggacgcg
      121 atcccccaga tgagggcggc caggatgagg agcgtcggcc gcctccaccc cgggcgcctc
      181 cgagtgctcg acgtgaagct gttggtcatc tgaactcctt ccggtctcta gctgcgcgcc
      241 atccgaagcg ctccctccga ttcggaggga caccctcgtt tcgaggggtt actgcccagg
      301 agatcgccct cgttggcccg gcgccgtgcc atttcgtcac cttcgccggt tgcgttagcg
      361 ataacgcgaa ttgctagtgc ttggaggcta gcacgggtcg ggagggtggt caaggtcgag
      421 ccgaggcgag gccggacacc gtatcaccct ccacaacgcc cctcccgctc gcgtcctagg
      481 gacgtcctgg ttgacgggga ccggctgcgg gcgggccgcg gacgggccgc ggacggccgg
      541 ccatgtttag cagggctctg cgtgctcggc agggctctgc ttgctcgggg acctgctgcg
      601 gacgggctgc ggacggactc cggacgggcc tggtcaccta gcctcataca acgaggccga
      661 cccgggctga gcgcctgtag gcctctcacc cccaacgccg cgccgctcgc gtcggcgtca
      721 gaaggcaggg aaccgacgtg agaaagcagg gaaccgacgt gagaccactt ctgatcgcga
      781 cagccctcgg agcactgatc ctcgccgcct gcagcccgcc gcagccggac ccgacaccgg
      841 acccgctggt gttcggggtc ccgtccgttc tgacggccaa ccccggccaa gcagcgcagt
      901 tcgcggcgga gctcgagcgg cgcctcgacc gtcccgtcac cgtgcagacc gtcgaccggg
      961 ccgtcgatct gacgcagtcg ctggaggacg gcaccttcga cttcgcccac ctcccaccct
     1021 cgatgaccgc gcagaccatc gccgccctgg agctccccct ggtcgccatc gtctctcgcg
     1081 gaggcggaac cacctacagg gcgcagtaca acgccaggtg cgacctaggg ctgaccggtc
     1141 tgggcgatct ggcgggccta cggttcggct tcgtatccaa ccgctcggcc tcgggtcatc
     1201 tcgttccata cctggagctg accgacgcgg gcatcgaccc ggaagtggac ctgaccacct
     1261 ccttcgcagg cagccacgac gcggtgatac ttgggatcta cgcgaacgat ctggacgcgg
     1321 gcgtgtcctg ggaagacgca cgcgtggcgg tctccggtga ccaccccgac gtgaacgacg
     1381 tggtgtgcgt catcggctac tccacgccac tccccaacga cgcggtgctc gccagagccg
     1441 gcctaggcgc agacgtg
//
LOCUS       DAOQYE010000665         1455 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_111487_length_1455_cov_3.343571, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000665 DAOQYE010000000
VERSION     DAOQYE010000665.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111487_length_1455_cov_3.343571"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="PKN52_09965"
     CDS             complement(<1..415)
                     /locus_tag="PKN52_09965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00318.1"
                     /translation="MSAAVRAAFTGGGVPTSPYLAHAKVNLGLAVIALRGDGYHELET
                     VMARIGLADELTVVVAEAGESHDSVRLSVEVAPGLPGSGAIGSAEDNLATRAAYAYLR
                     ARRTWTPAAPFVRIDLRLVKRIPIAAGLGGGSADAG"
     gene            complement(412..1080)
                     /locus_tag="PKN52_09970"
     CDS             complement(412..1080)
                     /locus_tag="PKN52_09970"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IspD/TarI family cytidylyltransferase"
                     /protein_id="HNR00319.1"
                     /translation="MSRKAGLVAAAGSGTRMGLGPKAFVELGGATLLELAVANVRAVV
                     DEVVVAVPAALLGEARALLGASVALVSGGDSRQATVRAMLAEAAGDVVLVHDAARPFL
                     PREVGERVLAAAVETGAASACLRVTDTVVNIVDGRALARDELLRVQTPQAFARDLLER
                     AHAVAAADGVEATDDAALVRRLGVDVAWVEGSPLLHKLTTVEDLELGLALLHVWRSRG
                     GGGA"
     gene            complement(1077..>1455)
                     /locus_tag="PKN52_09975"
     CDS             complement(1077..>1455)
                     /locus_tag="PKN52_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HNR00320.1"
                     /translation="DSDLGAKISSGSHPTAGMVIVPCSASTLAKVSQGHADTLITRAA
                     HVTLKERRPLVLVLREAPYSRPMLVNMLAAHDAGATILPAAPGFYSGPTDIAALVAGV
                     TSRALDLLGVANDHAPRWRAGDA"
BASE COUNT          198 a          555 c          517 g          185 t
ORIGIN      
        1 tcccggcgtc ggccgagccg cctcccaggc ccgctgcgat cgggatgcgc ttcacgaggc
       61 ggagatctat gcgcacgaac ggcgccgccg gcgtccaggt ccgccgtgcc cgcaggtacg
      121 cgtacgccgc ccgggtcgcc aggttgtcct cggccgaccc gatggcgccg ctgccaggca
      181 ggccaggcgc tacctccacg ctcaaccgca ccgagtcgtg gctctcgccg gcctccgcga
      241 ccacgaccgt gagttcgtca gcgaggccga tcctggccat caccgtctcc agctcgtggt
      301 agccgtcgcc ccgcaaggct atgacggcca gaccgaggtt gaccttggcg tgcgccaggt
      361 aagggctggt agggacgccg cctccggtga aggcggcccg gaccgccgcg ctcacgcgcc
      421 tcccccgcct ctggaacgcc acacgtgcaa cagcgcaagc cccagctcca ggtcctcgac
      481 cgtcgtcagc ttgtgaagca gcgggctccc ctcgacccat gcgacgtcga cgcccagccg
      541 cctcacgagg gcggcgtcgt cggtcgcctc caccccgtcg gcggccgcga cggcgtgggc
      601 acgctccagg aggtcgcgcg cgaacgcctg cggggtctgg acgcgcagca gctcgtcgcg
      661 cgccagggcc cgaccgtcga cgatgttgac gaccgtgtct gtgacgcgca ggcacgcgct
      721 ggccgcgccc gtctcgacgg cggcggccaa cactcgctcg ccgacctccc gcgggaggaa
      781 ggggcgggcg gcgtcgtgta cgaggacgac gtcgccggcg gcctcggcca acatggcgcg
      841 cacggtggcc tgccgcgagt cgccacccga caccagcgcg accgacgcgc cgaggagcgc
      901 ccgcgcctcg cccagcagcg cggccggcac ggccaccacg acctcgtcga ccacggcgcg
      961 cacgttggcg acggcgagct cgaggagggt ggctcccccc aactcgacga acgccttcgg
     1021 tccgagcccc atgcgggttc cgcttccggc cgccgcgacg aggcccgcct ttcggctcat
     1081 gcgtcccccg cgcgccagcg tggcgcatgg tcgttggcca cgccgagcag gtcgagcgcg
     1141 cggctggtga ccccggccac gagcgcggcg atgtctgtcg ggccgctata gaagccgggc
     1201 gcggccggaa ggatggtggc gcccgcgtcg tgcgccgcaa gcatgttcac cagcatcggc
     1261 cgcgagtacg gagcctcgcg cagtaccagc accagcggcc ggcgctcctt gagcgtcacg
     1321 tgagccgcgc gcgtgatgag agtgtcggcg tggccctggg agaccttggc gagggtgctg
     1381 gcgctgcacg gcacgatgac catgcccgca gtcgggtggc tcccagagga gatctttgcg
     1441 ccgaggtcgg agtcc
//
LOCUS       DAOQYE010000666         1455 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_111547_length_1455_cov_2.177143, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000666 DAOQYE010000000
VERSION     DAOQYE010000666.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111547_length_1455_cov_2.177143"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="PKN52_09980"
     CDS             complement(<1..453)
                     /locus_tag="PKN52_09980"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177567.1"
                     /note="catalyzes the transfer of phosphate between the C1
                     and C5 carbons of pentose; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HNR00321.1"
                     /translation="MTVTLVVLDSVGVGALPDAADFGSAGGNDAGSHTLDHVVATTGV
                     ELPHLAELGLANIEGVRSLPRSTSPAASYGRLAELSVGKDTTTGHWEFMGTVVEHPFR
                     TFERFPEAVMREFDAATGRGHLGNYPASGTTILDELGPEHLRTGYPIVY"
     gene            548..>1455
                     /gene="tilS"
                     /locus_tag="PKN52_09985"
     CDS             548..>1455
                     /gene="tilS"
                     /locus_tag="PKN52_09985"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HNR00322.1"
                     /translation="MAKPVDLPANVDAELTRLAPGAGRVVVAVSGGADSVAAACLLAV
                     AGRDLVIAHFDHQLRPASGQDAEFVRDLSARLGAEFRGGAADVAAVARAKRWNLEDAA
                     RRLRYAFLHRVLREVAARDGVIVVAHTRDDQAETFLLQLLRGAAFPGGMADRRGSVVR
                     PLLGTGRDELRAYLRRAAQTWREDATNADVSRNRAWVRAELLPRLEGRFPGVTPRIAH
                     TASQLAAARTTLERVARERFGEGDVAVVRLAGAEPALRRAAVAERLRALGVPVTRAAM
                     ADIEDALAAAARLGVGAPPWRRDLPRG"
BASE COUNT          182 a          498 c          580 g          195 t
ORIGIN      
        1 gtagacgatg gggtagccgg tgcgcaggtg ctcggggccg agctcgtcta ggatggtcgt
       61 gccggaggcc gggtagttgc caaggtgacc gcggccggtg gccgcgtcga actcgcgcat
      121 cacggcctca gggaagcgct cgaacgtgcg gaacggatgc tctacgaccg tgcccatgaa
      181 ctcccaatgc ccggtggtcg tgtccttgcc gacggagagc tctgccagcc ggccgtagct
      241 ggcggcgggc gaggtcgagc gcggcaggct cctgacgccc tcgatgttgg cgaggcccag
      301 ttcggccagg tgcggcagct cgacccccgt cgttgcgacc acgtgatcga gggtgtggga
      361 accggcgtcg ttcccgccgg cggagccgaa gtcggccgcg tcgggtaggg cgccgacccc
      421 gacggagtcg agtacgacta acgtcacggt catggcgcga gtataagagc ccgcccggct
      481 cccgcatacc acgtgccgtg gggaggagcc gagttcccca agccgcgggc cgggcgataa
      541 tgcgaccgtg gcgaaaccgg tggacctccc agccaacgtg gacgcggagc tgacgcgctt
      601 ggcgcccggc gccgggcgcg tggtcgtggc cgtgtcgggc ggggcggact ccgtcgcggc
      661 ggcgtgcctc ctagccgtgg ccggccgcga cctggtgatc gcccacttcg accatcaact
      721 gagacccgcc tctgggcagg acgccgagtt cgtgagggac ctgagcgcgc ggctcggggc
      781 ggagttccgc ggcggggctg ctgacgtcgc ggcggtggcg cgtgccaagc gctggaacct
      841 ggaggacgcc gcccgtcggc tgcgctacgc gttcctgcac agggtcctgc gcgaggtggc
      901 cgctcgcgac ggcgtgatcg tggtcgcgca cactcgtgat gaccaggcgg agacgttcct
      961 gctccagctc ctcaggggcg ccgcgttccc gggcgggatg gcggaccggc gggggagcgt
     1021 cgtcaggccg ctccttggga caggccgcga tgagctccgc gcctacctgc gccgcgcggc
     1081 gcagacatgg cgcgaggacg ccaccaacgc cgacgtcagc cgcaaccgcg cctgggtccg
     1141 cgccgagctg ctgcccaggc tcgaggggcg tttcccgggc gtgaccccgc gcatcgcgca
     1201 tacggcctcg cagctcgccg cggcgagaac gacgctggag cgcgtggcgc gcgagcggtt
     1261 cggggagggc gacgtggccg tcgtccgcct ggccggggcc gagccggcgt tgcggcgcgc
     1321 ggccgtcgcg gagcggttgc gcgcgctcgg cgtgccggtc acgcgggcgg ccatggccga
     1381 catcgaggac gcgctggccg cggcagctcg gctcggcgtc ggcgccccgc cgtggcgccg
     1441 ggacctgccg agggg
//
LOCUS       DAOQYE010000667         1454 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_111648_length_1454_cov_2.628306, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000667 DAOQYE010000000
VERSION     DAOQYE010000667.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1454
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111648_length_1454_cov_2.628306"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..788)
                     /locus_tag="PKN52_09990"
     CDS             complement(<1..788)
                     /locus_tag="PKN52_09990"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HemK/PrmC family methyltransferase"
                     /protein_id="HNR00323.1"
                     /translation="MASRTVPEAHTAAAKQLAAAGVPSPEADAWRLLEAATGWSRSAL
                     LLNGAKPLDDAAVERLTLLVERRAARVPLQHILGSADFYGLELAVDGRVLVPRPETER
                     LVELVLHELARQDSARHDSARHDSARHALARSAGSTPQLMIDVGTGSGAVALAVKAER
                     PGTQVVGTDISEDALTVARGNAARLGLEVGFARSDLLREEAVAALARRAAVLAANLPY
                     LPETDRGSLQPEAAADPPGALFAADGGLALAARLAEQAWSTLPRG"
     gene            complement(793..1383)
                     /locus_tag="PKN52_09995"
     CDS             complement(793..1383)
                     /locus_tag="PKN52_09995"
                     /inference="COORDINATES: protein motif:HMM:NF024483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HNR00324.1"
                     /translation="MSDSDLDKYLEGIGIDVDGEDAEEAPVTRFAAEEEYEQVVDAGP
                     IVQGEPVERAEAFLVNLLLNIDPAYAVEVEQASDDEIQAEVYGGDPGKIIGRNGRTLA
                     ALEYLTNAVVNRDESGRIRVNVDVGGYKRRRDERLRKEAMQAAARVRKGGMPVELEPM
                     SAAERRVVHMCLAEEPGVSTESRGEGSDRRVVVRPA"
BASE COUNT          198 a          539 c          501 g          216 t
ORIGIN      
        1 ccgcgcggca aggtcgacca ggcctgctca gcgagtcgcg ccgccagggc caggccgcca
       61 tcggcggcga agagcgcgcc cggcgggtcg gcggccgcct ccggctgcag gctacccctg
      121 tcggtctcgg ggaggtaggg caggttggcc gcgagcacgg cggcgcgccg ggcgagagcg
      181 gcaacggctt cctctcgcag gaggtcggag cgcgcgaagc cgacctcgag gcctagcctg
      241 gccgcgttgc cgcgcgccac agtgagcgcg tcctccgaga tatcggtgcc caccacctgg
      301 gtgcccgggc gctcagcttt cacggcgagc gcgacggcgc cgctgcccgt gccgacgtcg
      361 atcatcagtt gcggagtcga gcccgcagag cgcgccaaag catggcgcgc cgaatcatgg
      421 cgcgccgaat catggcgagc cgagtcttgg cgcgccaact catgcagtac gagttccacg
      481 agccgctccg tctcgggccg cggcacgagc acgcgcccgt cgacggccaa ctcgagcccg
      541 tagaagtcgg cgctgccgag gatgtgctgc agcggcacgc gcgcggcgcg cctctcgacg
      601 agaagcgtca gccgctcgac agccgcgtcg tcgaggggct tcgcaccgtt caggaggagc
      661 gcgctacgac tccaccccgt ggcggcctcc aagagccgcc aggcatctgc ttccggactg
      721 ggtacgcccg ccgccgcgag ctgcttggcc gcggccgtgt gggcttcggg gacggtgcgc
      781 gaggccacgc ccctaagcgg gcctgaccac cacgcgcctg tcgctgccct cgccgcgcga
      841 ctccgtgctc accccgggct cctctgccag gcacatgtgc acgacgcgcc gctcggccgc
      901 gctcatcggc tcgagttcga cgggcatgcc gcccttgcga acccgggcag cggcctgcat
      961 ggcttccttg cgcagtcgct cgtcacggcg gcgcttgtag cccccgacat cgacgttgac
     1021 gcggatccga ccgctctcgt cgcggttcac gacggcgttc gtcaggtact cgagcgccgc
     1081 caacgtgcgc ccgttgcgcc cgatgatctt gccagggtcg ccgccgtaga cctccgcctg
     1141 gatctcatcg tccgacgctt gctcgacctc gacggcgtac gccgggtcga tgttcaggag
     1201 caagttgacc aggaacgcct cggcgcgctc gacaggctcg ccctggacga tcgggccggc
     1261 atcgacgacc tgctcgtact cctcttcggc ggcgaagcgg gttaccggcg cctcttccgc
     1321 gtcctccccg tcgacatcga tgccgatgcc ctctaggtac ttgtcgagat ccgagtcact
     1381 catctatggc ctcacttgga gcgcttggcc ggcgcggcga caggcaatcg gagccggcgg
     1441 gcgcgggcac cggt
//
LOCUS       DAOQYE010000668         1453 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_111829_length_1453_cov_2.072961, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000668 DAOQYE010000000
VERSION     DAOQYE010000668.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1453
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111829_length_1453_cov_2.072961"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..787)
                     /locus_tag="PKN52_10000"
     CDS             complement(<1..787)
                     /locus_tag="PKN52_10000"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNR00325.1"
                     /translation="MEANQQAESPVDAVHAPECVICGTSLAGPLGFLFRLVGIRRSTR
                     NPNLCSRCNAHVEEGRVVELTVLFADLSSFTELTQQLGPQQTHAVVDAFLKTATYVLV
                     RHGAFIDKYVGDAVMAFFNAPIRYPDHAARATAAALEIQAELPALGERFGLDLRASIG
                     VASGFAHVGRLGSGDGKDYTAIGDVVNLAARLQSAAGAGEVVVDAPVFAQVADQFPAV
                     VAERIPLKGFAAPVDAHRLGNAEVVAQPNAGRRYDIGTERPNRA"
     gene            977..1327
                     /locus_tag="PKN52_10005"
     CDS             977..1327
                     /locus_tag="PKN52_10005"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNR00326.1"
                     /translation="MGSSAVLDATFAALADPTRRAILARLAAGEASVAELAAPFAMSQ
                     PAVSRHLKVLERAGLISQGRDAQRRPRRLEAAPLAEATAWLENYRQFWEGQYQRLDAL
                     LDELQGLTPEQPGS"
BASE COUNT          218 a          516 c          497 g          222 t
ORIGIN      
        1 cggcgcggtt ggggcgttcg gtgccgatgt cgtagcgccg gccggcgttg ggctgggcga
       61 cgacttcggc attgcccagc cggtgcgcat cgaccggcgc ggcgaacccc ttgagcggga
      121 tccgttcggc gacgacggcc gggaactggt cggccacctg ggcgaagacc ggggcatcga
      181 ccacgacctc gccggccccg gccgcgctct ggagccgcgc cgcgaggttc accacgtcgc
      241 cgatggccgt gtagtccttg ccgtcgccgg aaccgaggcg ccccacgtgc gcgaagcccg
      301 acgccacgcc gatggaggcc cgcagatcca gcccgaaccg ctccccgagc gccggtagct
      361 cggcctggat ctccagtgcc gccgccgtcg cccgcgccgc gtggtccgga tagcggatgg
      421 gcgcgttgaa gaacgccatc acggcgtcgc ccacgtactt gtcgatgaac gcgccgtggc
      481 gcacgagcac gtacgtggcc gtcttcaaga aggcatccac gacggcgtgt gtctgctgcg
      541 gcccaagctg ctgggtgagt tcggtgaacg aactgaggtc ggcgaacagc accgtcagtt
      601 ccacgacccg cccttcctcg acgtgggcgt tgcagcggct gcacaggttc gggttgcgcg
      661 tgctgcgcct gatgccgacg aggcggaaca ggaagcccag cggcccggcg agcgaggtgc
      721 cgcagatgac gcactcgggg gcgtggaccg catcgacggg cgactcggct tgctggttgg
      781 cctccatctg cgcacctccc gcactgcgga cgagcgtacc acgcgcgggg tgacgaatcc
      841 cttacgacca tctccaaaga gactggaacg gccagcagca gcggacaggc gcaggctgcc
      901 gtaaggacgc cggagttgac ggcggccggc gccagcatgt atataatgca tgcgttatag
      961 aaagagagtg ttatgcatgg gatcgtccgc cgtccttgac gccacctttg ccgctctggc
     1021 cgacccgacg cgccgggcga tcctcgcgcg gctcgcggcc ggtgaggcgt ccgttgcgga
     1081 gcttgccgcg cccttcgcga tgagccagcc cgccgtctcc cgccacctga aagtgctcga
     1141 acgggccggc ctgatctcgc aagggcggga tgcccagcgc cggccgcgcc ggttggaggc
     1201 cgcgccactc gccgaggcga cggcctggct cgagaactac cgccagttct gggaaggtca
     1261 gtaccagcgg ctcgacgccc tgctggacga gctccagggg ctgaccccgg agcagccggg
     1321 ttcctgatcg tcccgccggc cccctccttc gcctccgcgc ggacctgctg gctgctcgtc
     1381 ccaccttcgt ctcgtcattc aacgtctcgc catccgacaa cctatctctc gccccgagga
     1441 gcatcttcga tga
//
LOCUS       DAOQYE010000669         1452 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_111918_length_1452_cov_2.566929, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000669 DAOQYE010000000
VERSION     DAOQYE010000669.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1452
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111918_length_1452_cov_2.566929"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..276
                     /gene="rpsB"
                     /locus_tag="PKN52_10010"
                     /pseudo
     CDS             1..276
                     /gene="rpsB"
                     /locus_tag="PKN52_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285255.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
     gene            597..1190
                     /gene="tsf"
                     /locus_tag="PKN52_10015"
     CDS             597..1190
                     /gene="tsf"
                     /locus_tag="PKN52_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HNR00327.1"
                     /translation="MAVSTEMIKQLRAMTGAGMMDVKKALEDADADLNKAAELLRERG
                     IAKADKKADRAAKEGLVGHYLHHNGKIAVLVEVNCETDFVARNERFQELARNLAMHIA
                     MASPAYTRRDDVPEEVIAAEKHALQRQVEEEGKPANVAEKIVEGRLNKYLGEIVLLDQ
                     AYIKDDKKTIDTVVKEVAATLGENIQVGRFARIAIGE"
     gene            1200..>1452
                     /gene="pyrH"
                     /locus_tag="PKN52_10020"
     CDS             1200..>1452
                     /gene="pyrH"
                     /locus_tag="PKN52_10020"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179247.1"
                     /note="Catalyzes the phosphorylation of UMP to UDP;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HNR00328.1"
                     /translation="MRRVLLKLSGEFLAGPGGFGVSPEATAALAGEIAAALGRGVQLA
                     VVVGGGNFWRGAQHGKHMDPATADYVGMLGTVMNAIALQD"
BASE COUNT          282 a          489 c          503 g          178 t
ORIGIN      
        1 ctccaccgcc tgcagcgtta cctcggcggc atccgcgacc tgaaccgcct gccggacgcg
       61 ctgttcatca tcgacccggc caaggaggcc atcgcggtca aggaggccaa caagctcggc
      121 atcccggtcg tggcgctggc cgacacggac tccgaccccg acgtcctcga cttcatcatc
      181 ccgggcaacg acgacgccat ccgctccatc cagctcgtca cggcccgcct cgcggacgtg
      241 atcatcgaga tgcgcggcgg cgaggacgct ggggtgacga cagcctcccc ggaccacgag
      301 acgctccgca tgccgatgtc ggcctccgag cgcgccatca aggaagccgc ggccgtggcg
      361 gagggcgatt cgccagaagc gccgctggac gacgactcgg acgccgagta agcgcgttac
      421 ccgccgccgg ccctgagcgt cgcaggggcc ggagacgacc cgcctaggcg gcgaagagtg
      481 atggagtggg cggacggccg cgagaggccg ggcgctctag cgaggcccgt ccgggcggtg
      541 agcccacccg gacgtgggcg ccgccctagc gggcggcaac gaagggaaga tgcgagatgg
      601 cagtatcgac ggaaatgatc aagcagctca gggccatgac cggcgcgggc atgatggacg
      661 tcaagaaggc cctcgaggac gccgacgccg acctgaacaa ggcggccgag ctcctgcgcg
      721 agcgcggcat agcgaaggcc gacaagaagg ccgaccgcgc cgccaaggaa ggcctggtgg
      781 gccactacct ccaccacaac ggcaagatcg ccgttctcgt cgaggtcaac tgcgagaccg
      841 acttcgtggc gcgtaacgag cgcttccagg agctcgcgcg caacctggcg atgcacatcg
      901 ccatggcgag ccctgcttac acgcgccgcg acgacgtgcc agaagaggtc atcgcggccg
      961 agaagcacgc gctccagcgt caggtggagg aagagggcaa gcccgccaac gtggccgaga
     1021 agatcgtgga ggggcgcctc aacaagtacc tcggcgagat cgttctcctc gaccaggcgt
     1081 acatcaagga cgacaagaag accatcgaca cggtggtcaa ggaagtcgcc gccaccctgg
     1141 gcgagaacat ccaggtcggt cgcttcgccc gcatcgcgat aggcgagtaa gccaagcgca
     1201 tgcgacgagt actgctgaag ctctccggcg agttcctagc gggtccgggc ggcttcgggg
     1261 tgtcgccgga agcgacagca gctttggcag gagagatcgc cgcggccttg ggccgcggcg
     1321 ttcaactggc cgtcgtcgtg ggcggcggca acttctggcg gggcgcccag cacggcaagc
     1381 acatggaccc ggccacggcc gactacgtcg gcatgttggg caccgtcatg aacgccatcg
     1441 ccctgcagga cg
//
LOCUS       DAOQYE010000670         1452 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_111957_length_1452_cov_2.011453, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000670 DAOQYE010000000
VERSION     DAOQYE010000670.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1452
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111957_length_1452_cov_2.011453"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1054)
                     /locus_tag="PKN52_10025"
     CDS             complement(<1..1054)
                     /locus_tag="PKN52_10025"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNR00329.1"
                     /translation="MPPTVRFPMVDLQSEVAELWSELSPAVERVLRSGQFIGGPEVEA
                     LEREVAEYMGVRHVLGLNSGTDALLLGLEALGVGPGDEVITSPFSFFATSEAILRLGA
                     KPVFVDIDEATLNLDPALLDQAITKRTRVILPVHIFGLPADMSAIMAVAHQRAIPVLE
                     DCAQAFGARIGLGDGRGGGDRSVTPDTGQRVGRSVGSFGALGAFSLYPTKNFGAYGDG
                     GLVSTGSDDLAARLRSLRNHGSTPQEKYLHEGLGYNSRLDALQAAVLRVKLRRLEEWQ
                     ARRLERVAAYRAALAGLPGGDDGLVLPPDAPEHRYHQFTLRLLPERRTRFEDGLRARG
                     VAFTRFYPIPLTRQPLG"
     gene            1200..>1452
                     /locus_tag="PKN52_10030"
     CDS             1200..>1452
                     /locus_tag="PKN52_10030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00330.1"
                     /translation="MSTVRPNTVEKTASPRRVRGLVRILTLIAATLPAVAFAQKPLAL
                     TGTIPNPTQREGTVVAWLGHLDVSASGVVAYGTVGVAGGF"
BASE COUNT          230 a          499 c          512 g          211 t
ORIGIN      
        1 ccccaagcgg ctgccgcgtg agaggtatag gatagaagcg cgtgaacgcc acgccacgcg
       61 cacgtagacc gtcctcgaag cgcgtccggc gctcgggcag caggcgcagc gtgaactggt
      121 ggtagcggtg ctcgggagcg tcgggcggca agacgagccc gtcgtcgccc cctggcagcc
      181 ccgccagcgc cgcgcggtag gcggccacgc gctccagccg gcgggcctgc cactcctcaa
      241 ggcgccggag cttcacccgt aggacggctg cctgcagcgc gtcgaggcgc gagttgtagc
      301 cgaggccctc gtgcaggtac ttctcctgtg gcgtcgagcc gtggttgcgt aggctgcgaa
      361 gtcgcgccgc caagtcgtcc gacccggtgg agacgaggcc gccgtcgccg taggcgccga
      421 agttcttggt ggggtagagg ctgaaggccc cgagggcgcc gaaggagccg acgcttcggc
      481 cgacacgctg accggtgtct ggggtgacgc tccggtcacc gccgccgcgg ccgtcgccga
      541 gaccgatgcg ggctccgaac gcctgggcgc agtcttccag cacggggatc gcgcgctggt
      601 gcgccaccgc catgatcgcg ctcatgtccg ccggtaggcc gaagatatgc acggggagga
      661 tgacccgcgt gcgctttgtg atggcctggt ccagcagcgc aggatcgagg ttgagcgttg
      721 cctcgtctat gtcgacgaac accggcttgg cgccaaggcg caggatggcc tcgctcgtgg
      781 cgaagaagct gaagggggag gtgatgacct cgtcgcctgg accgacacct agcgcctcta
      841 ggcccaagag cagggcgtcc gtgcccgagt tgaggcccag cacgtggcgg acgcccatgt
      901 actcggcgac ctcgcgctcc agagcctcga cctccgggcc gccgatgaac tgtccgctcc
      961 gcaggacgcg ttcgacggcg ggggagagct cgctccacag ctccgccacc tcgctctgca
     1021 ggtcgaccat ggggaatctg acggtgggcg gcacgcgcac aagcataggg ccaaagcgcg
     1081 ccggcggtca ctagttggct cggaggtccg ctaatctgag gcccagccac tgaagaaacg
     1141 gtccatcgaa ggaccaatgc cccatcccgg ggccgagcgc tcctccctat actcgtcgca
     1201 tgtccaccgt ccggcccaat accgtcgaga agaccgcgtc gcctaggcgc gtacgcggtc
     1261 tcgtgcgcat cctcaccctg atcgccgcaa cgctaccggc ggtggcgttt gcccagaaac
     1321 cgctagccct gaccggtacc atcccgaacc ccacccagcg ggagggcacg gtcgtggcgt
     1381 ggctcggcca cctcgacgtg tcggcgagcg gcgtagtcgc ctacggcacg gtcggcgtag
     1441 cgggcgggtt cg
//
LOCUS       DAOQYE010000671         1451 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_112055_length_1451_cov_2.651146, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000671 DAOQYE010000000
VERSION     DAOQYE010000671.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112055_length_1451_cov_2.651146"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            137..1015
                     /locus_tag="PKN52_10035"
     CDS             137..1015
                     /locus_tag="PKN52_10035"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNR00331.1"
                     /translation="MDTIIALQRLASPALDRAMLLITDICSQEAFTVLLVLAFLAVDA
                     TFGRRLGIVFLAGAYVNDAIKTLVDAPRPFQVRPEVLRGSAADTAPGSSFPSGHAQSA
                     LTFWTLSALRFKRAWFWALAVLIIAAVALSRVYLGVHYPADVLGGLGVGLLVVIVAQF
                     AGRLSFAPGKAVVVTLGLLVPLVVHLVFTTEASHVFLAVASAFIVGPELVSHRASGGV
                     VTRLAMGVVGVALVAAVLSATSALMPEEVKHAPLPGYLRYLAIGFTGTVLAPLVCRWA
                     RLSGGAVVATGSAKRA"
     gene            1017..>1451
                     /locus_tag="PKN52_10040"
     CDS             1017..>1451
                     /locus_tag="PKN52_10040"
                     /inference="COORDINATES: protein motif:HMM:NF013575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00332.1"
                     /translation="MTDAPPLGRPEPGGTAKKRLRLTLEFDGSDFLGWQRQAGSERTV
                     QATIESAFAALPGEHSSVLGAGRTDAGVHALALVAHVDTTWDQPNEKLRLALNAHLPP
                     DVKVVAVADAAPDFQAQFDCLYRRYLYRMRVMRDDPRGMAIQR"
BASE COUNT          181 a          537 c          493 g          240 t
ORIGIN      
        1 gaggcgcgcc accgctccat gctcgcactc tcgcggcgtc ggtggcgtaa cgcgccgcgc
       61 ggcgccggcc cccgccggcg gcggcgttgg taaaatcgcg ccctagccgc gcgaacccgc
      121 gcactaggga ggcatagtgg acacgatcat cgccctgcaa cgactcgcgt cgccggccct
      181 cgacagggcg atgctcctga tcaccgacat ctgctcccaa gaagcgttca ccgtgctcct
      241 cgtcctcgcg ttcctggcgg tagacgcgac gttcgggcgg cgcctcggca tcgtgttcct
      301 ggccggcgcg tacgtgaacg acgcgatcaa gacgctcgtc gacgccccac gaccgttcca
      361 ggtgaggccg gaggtgctac gcggcagcgc ggccgacacc gcgccaggca gttcgttccc
      421 ctccggtcat gctcagtcgg cgctgacctt ctggacgctc tcggccctgc gtttcaaacg
      481 cgcgtggttc tgggcgttgg ccgtcctgat aatcgccgcg gtcgccctgt cgcgcgtcta
      541 ccttggcgtt cactacccag ccgacgtcct tggcggcctg ggcgtcggtc tactcgtggt
      601 gatcgtcgcc cagttcgcgg ggcgcctgtc cttcgcgcca ggcaaggctg tcgtggtgac
      661 gctcggtctg ctcgttccac tggtcgtcca cctcgtcttc acgacggagg cgtcgcacgt
      721 cttcctagcg gtcgcctcgg cgttcatcgt cggccccgag ctcgtgtcgc accgcgccag
      781 cggcggggtc gtgacgcgtc tggcgatggg cgtcgtcggg gtggcgctgg tggccgcggt
      841 gctgagcgcc acatcggcgc tcatgcctga ggaggtgaag catgcgccgc taccgggtta
      901 cctgcggtac ctggcgatcg gcttcacagg caccgtcctg gccccgctcg tgtgtcgctg
      961 ggcgcggctg agcggcggcg ccgtcgtggc gaccggcagc gcgaagcgcg cctgacgtga
     1021 ccgacgcgcc gcccctgggg cgcccggagc ctggcggaac ggccaagaag cgcctgcgac
     1081 tcaccctcga gttcgacggc agcgacttcc ttggctggca gcgccaggcc gggagcgagc
     1141 gcacggtgca ggccacgatc gagtccgcct tcgccgccct gcccggcgag cactcgagcg
     1201 tgctcggcgc cggtcgtacc gacgccggcg tgcatgccct cgcgctggtg gcgcacgtcg
     1261 acaccacctg ggaccagccc aacgagaagc tgcgcctggc actcaacgcc cacctgccgc
     1321 ccgacgtcaa ggtcgtcgcg gtcgccgacg ccgccccgga cttccaggcg cagttcgact
     1381 gcctctatcg gcgctacctc taccggatgc gcgtgatgcg cgacgacccg cgcggcatgg
     1441 ccattcagcg c
//
LOCUS       DAOQYE010000672         1450 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_112191_length_1450_cov_2.510394, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000672 DAOQYE010000000
VERSION     DAOQYE010000672.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1450
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112191_length_1450_cov_2.510394"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /gene="ansA"
                     /locus_tag="PKN52_10045"
     CDS             <1..193
                     /gene="ansA"
                     /locus_tag="PKN52_10045"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178244.1"
                     /note="converts asparagine to aspartate and ammonia;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HNR00333.1"
                     /translation="LRGRVDMRGYATVAALAEAGVTGGADMTPEAALTKLIYLLGSRM
                     TPAQARAAMARDLRGELSA"
     gene            190..597
                     /locus_tag="PKN52_10050"
     CDS             190..597
                     /locus_tag="PKN52_10050"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HNR00334.1"
                     /translation="MSAAARAPWRERPRWLRWLGWGLAVAWAGFLFMESSSSTAGSFL
                     SNFPPGSDKVVHAGAFGMLAALVTLGSGRPLLGVLAALLYGISDEIHQWFVPERATEV
                     LDVVADTVGGLLGAFAVDFLARRRHRNSVGRSV"
     gene            606..1433
                     /gene="proC"
                     /locus_tag="PKN52_10055"
     CDS             606..1433
                     /gene="proC"
                     /locus_tag="PKN52_10055"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HNR00335.1"
                     /translation="MSGTSPESRARTGPRLVIVGAGKMGGAVLQGAIAAGALERSDVA
                     IFHPNERRLAELSERFGVAGVDVAGLHEAEYVLLGTKPQSFATVAPVVATQNAAFISL
                     MAGVTLETIARLVGSKRVIRAMPNLGASLGVGATALAWLPESTSEDVALARRLFGAVG
                     RVYDLREELFDAFTGMAGSGPAYVAVVAEALADGGVRVGLDRSLARDVARQLLLASAR
                     LLETKYPSELKDEVSSAGGTAIAGVRTLERHRLRFALMDAIEEATRRAGELSRQEGK"
BASE COUNT          181 a          516 c          527 g          226 t
ORIGIN      
        1 cctccgtggc cgcgtcgaca tgcgtggcta cgccacggtc gcggcgctcg ccgaggcggg
       61 cgtgacgggc ggcgccgaca tgacgccaga ggccgcgctc accaagctga tctacctgtt
      121 ggggagccgc atgacacctg cccaggcgcg cgccgcgatg gcccgcgacc tgcgcggcga
      181 gctgagcgcg tgagcgccgc cgcacgcgcc ccgtggcgcg agcgcccgcg ctggctgcgg
      241 tggctcggct ggggcctcgc tgtcgcctgg gcgggcttcc tcttcatgga gtcgagttcg
      301 tctaccgccg ggtcgttcct gtccaacttc ccgcccggct cggacaaggt cgttcacgcg
      361 ggggcgttcg gcatgttggc cgccctcgtc accctgggga gcggcaggcc gctactgggc
      421 gtgctcgcgg ccctcctgta cggcatctcg gacgagatcc accaatggtt cgtacccgag
      481 cgcgccacgg aggtgctcga cgtcgtggct gacacggtgg gtggcctgct tggcgccttc
      541 gccgtcgact tcctagcccg ccggcggcac cgcaactcgg taggcaggtc agtatgattg
      601 acgccatgtc aggaaccagc cccgaatcgc gcgcccgcac gggcccgcgg ttggtcatag
      661 tcggtgccgg caagatgggc ggggccgtgc tgcagggcgc catcgccgct ggggcgctcg
      721 agcggtccga tgtagccatc ttccacccga acgaacggcg cctcgccgaa ctatcggagc
      781 gcttcggggt agcgggtgtc gacgtcgccg ggctgcacga ggctgagtac gtgctccttg
      841 gtaccaagcc gcagtcgttc gcgaccgtgg cgcccgtggt cgccacgcag aacgcggcgt
      901 tcatctcgct gatggccggg gtgaccctcg agaccatcgc gcgtctggtc ggctcgaagc
      961 gcgtcatccg cgccatgccc aacctgggcg cgagcctcgg agtcggcgcc acggcgctcg
     1021 cctggttgcc cgaatcgacc tcggaggacg tggccctcgc gcgccgcctg ttcggggccg
     1081 tcggccgcgt ctacgacctg cgcgaggagc tgttcgacgc cttcaccggg atggccggct
     1141 ccggccccgc ttacgtcgct gtcgtcgccg aggcgctagc ggacggcggc gtgcgcgttg
     1201 gcctcgaccg ctccctcgcc cgcgacgtgg cgcgccagtt gctcctggcg tccgcgcgcc
     1261 tcctggagac gaagtacccg agcgagttga aggacgaggt gtcgtccgcc ggcggaacgg
     1321 ccatcgcggg ggtgcgcacg ctcgaacgcc accgcctgcg cttcgccctc atggacgcca
     1381 tcgaggaagc gacgcgccgg gccggagagc tgagccgcca ggagggcaag tagatggcgc
     1441 ggcttcgagg
//
LOCUS       DAOQYE010000673         1448 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_112503_length_1448_cov_1.987796, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000673 DAOQYE010000000
VERSION     DAOQYE010000673.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1448
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112503_length_1448_cov_1.987796"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="PKN52_10060"
     CDS             complement(<1..274)
                     /locus_tag="PKN52_10060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00336.1"
                     /translation="MPLSRRAALHALTTVGAGLAASPALRALRALAHETADRAPHDLA
                     ADQDYWDTIRRAYDITPDFVQLENGYFSPCAREVHERYLAHLRMVNA"
     gene            complement(278..640)
                     /locus_tag="PKN52_10065"
     CDS             complement(278..640)
                     /locus_tag="PKN52_10065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00337.1"
                     /translation="MPLLVLVVALIAAAAMAGAWYGRLQERAWWTRRLLERGVNPDTL
                     YPARVQTPLEPLTPETDAMREAMEDMQREVEQLAEGQRFLTQILTERRQLPTPPASPP
                     ATPPGMGDERPSPADRRS"
     gene            824..>1448
                     /locus_tag="PKN52_10070"
     CDS             824..>1448
                     /locus_tag="PKN52_10070"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HNR00338.1"
                     /translation="MPLLLRAIPFPLAFAPAHRAGGAVARQREGRGRTHWGWLALLLA
                     VSAAVVATPAAAQSRHIHGVLEQAGTGTPVDDAEVTVTWVIGRGQQQRRTRSDDAGRF
                     DFRDLPAQRVVLRVRRLGFVPLERTVDLAGDDALLKLLLTESPVRLSDVTIQADTVVE
                     ALERVAAVSTLDREALAATRGQTLGETLKHLPGVSVIQLGPSIAKPVI"
BASE COUNT          196 a          494 c          558 g          200 t
ORIGIN      
        1 gtgcgttgac catgcgcagg tgcgcgaggt agcgctcgtg cacttcgcgc gcgcacggcg
       61 agaagtagcc gttctccagc tggacgaagt cgggcgtgat gtcgtaggcg cggcggatcg
      121 tgtcccagta gtcctggtcg gcggcgaggt cgtgcggagc gcggtcggcc gtctcgtggg
      181 cgagcgcgcg cagggcgcgt aacgcgggcg aagccgcgag gccggcgccg acggtggtca
      241 gcgcgtgcag ggcggcgcga cgcgagaggg gcatgcctca gctccggcga tcggcggggg
      301 aggggcgctc gtcgcccatg cccggcgggg tggcgggcgg cgacgcgggc ggcgtgggga
      361 gctggcggcg ctccgtgagg atctgcgtga ggaagcgctg cccctcggcc aactgctcga
      421 cctcgcgctg catgtcctcc atggcctcgc gcatcgcgtc cgtctcgggc gtgagcggct
      481 cgagcggcgt ttgcacccgg gcggggtaca aggtgtcggg gttcacgcca cgttcgagca
      541 ggcgccgagt ccaccaggcg cgctcctgca ggcgaccgta ccaggcgccg gccatggcgg
      601 ccgcggcgat gagcgcaacg acgaggacga gcaggggcat ggggccagac gggcgaaggc
      661 gcagatcggt ccgcgcgcgt cagcgcggcg cgggcgtcaa ccgtttcccg aacctctgga
      721 agctacaccg ccacccagcg gaggcctagt aatgattacg gcaaatcatt atcctgcatc
      781 atatacaccg tacccctcag gtgcggtcga ccgacgactc ccgatgccgc tcctgctccg
      841 cgccataccg ttcccgctcg cgttcgcgcc cgctcatcga gcgggcggtg ccgtcgcgcg
      901 gcagcgcgag ggacgcgggc ggacgcattg ggggtggctg gcgctcctgc tggcggtgag
      961 tgccgccgtc gtggccacgc ccgcagctgc ccagagccgg cacatccacg gcgtgctcga
     1021 gcaggccggc accggcacgc cggtggacga cgccgaggtg accgtgacgt gggtgatcgg
     1081 gcgcgggcag cagcagcggc gcacccggtc ggacgacgcc ggccgtttcg actttcgtga
     1141 cctgcccgcg cagcgcgtgg tcctgcgagt gcgccggctc ggcttcgtgc cgctcgagcg
     1201 cacggtcgac ctcgcgggcg acgatgcgct gctcaagctc ctgctcaccg agagcccggt
     1261 gcgcctgtcg gacgtcacca tccaggcgga caccgtcgtg gaggcgctcg agcgtgtcgc
     1321 ggcggtgagc acgctcgacc gcgaggcgct cgcggccacg cgcggccaga cgctcggcga
     1381 aacgctcaag cacctgccag gcgtgtcggt catccagctc gggccgagca tcgcgaagcc
     1441 ggtcattc
//
LOCUS       DAOQYE010000674         1446 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_112733_length_1446_cov_2.469446, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000674 DAOQYE010000000
VERSION     DAOQYE010000674.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1446
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112733_length_1446_cov_2.469446"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..636
                     /locus_tag="PKN52_10075"
     CDS             <1..636
                     /locus_tag="PKN52_10075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00339.1"
                     /translation="RTLIVTDERAAEVLTDPTTLRQIAPFLGRTLSVGEAARETGEKA
                     NTTLKRVQRFEQLGLLEVAEAVPRAGRAVKRYRSTADVFFVPFEATHAESLEAALAER
                     DAYWERLLRRNVVRGRREALGDWGTRFYRDERGHLQVQTAVTPEANATTLDADAPATL
                     SLWRDQLQLDFADAKELQREMFALVQRYQRRSGAQRYLVRMGLAPVLDAPA"
     gene            751..1098
                     /locus_tag="PKN52_10080"
     CDS             751..1098
                     /locus_tag="PKN52_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00340.1"
                     /translation="MLAALTRSVLLMALVAVLGACAPGAFGPAASTDPVATELERTAQ
                     VLYHRMELGRLETGVYTTNALIDVTLPEGARWTLQEFATDGSSYVLVLRSSRLPSMAW
                     RVSPRGVSRFDPG"
     gene            complement(1192..>1446)
                     /gene="trxA"
                     /locus_tag="PKN52_10085"
     CDS             complement(1192..>1446)
                     /gene="trxA"
                     /locus_tag="PKN52_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNR00341.1"
                     /translation="WAPWCGPCRMVGPVIEELASDYAGKVKVGKLNVDDNMRVAQEYR
                     VMSIPTVMLFKDGKPVEVMVGAQSKANYEARLKKHVPATA"
BASE COUNT          216 a          523 c          493 g          214 t
ORIGIN      
        1 cgcaccctga tcgtcacgga cgaacgcgcc gcggaggtcc tcaccgaccc gacgaccctc
       61 aggcagatcg cgcccttcct tggccgcacg ctgagcgtcg gcgaggcggc gcgagagacg
      121 ggcgagaagg ccaacacgac gctgaaacgc gtgcaacgct tcgagcagct cgggctcctc
      181 gaggtggcgg aggcggtgcc gcgggccggg cgcgccgtca agcgttaccg ctccactgcc
      241 gatgtcttct tcgtcccctt cgaggcaacg cacgccgagt cgctggaggc agcgcttgcc
      301 gagcgcgacg cgtactggga gcgcctgcta cggcgcaacg tcgtgcgcgg caggcgcgag
      361 gcgctcggcg attggggcac gcgcttctac cgagacgagc gcgggcacct gcaggtccag
      421 acggccgtga cgccggaggc caacgccacg acgctcgacg ccgacgcccc cgccacgttg
      481 tcgctctggc gcgaccagct ccaactcgat ttcgcggacg ccaaggagtt gcagcgcgag
      541 atgttcgcgc tcgtgcagcg ctaccagcgc cgatcgggcg cgcagcgcta cctggtgcgc
      601 atgggtctcg cgccggtcct cgacgcgccg gcttgagcgc cacggcgctc accctggtcc
      661 cacgcgcacc gttaacatga gggcatggga gcgcttactg caaccgcacg agccaccggc
      721 cgcggcgcga cgacctcaca agcccggccg atgctagccg ccctgacaag gtcggtcctc
      781 ctcatggcgc tggtggcggt gctcggcgcc tgcgcccctg gcgcgttcgg ccccgccgcc
      841 tccaccgacc cggtggcgac ggaactcgaa cgcaccgccc aggtcctcta ccaccgcatg
      901 gagctcgggc gcctggagac cggcgtctac acgaccaacg cgctgataga cgtcacgctg
      961 ccggaagggg cacggtggac tttgcaggag ttcgccaccg acggctccag ttacgtgctc
     1021 gtgctgcgct cctcgcgcct gccgagcatg gcgtggcgcg tgtcgccgag gggcgtgagc
     1081 cgcttcgatc cgggttgagg gcgtcggccg cccaaccgaa gcggaagggc ccggagagat
     1141 ccgggccctt cctccatctg cgccgttcac ggccgcagcc gcagcggcta ctcaggcggt
     1201 ggcgggcacg tgcttcttga gccgagcctc gtagttcgcc ttcgattgcg caccgaccat
     1261 gacctcgacg ggcttgccgt ccttgaacag catgaccgtt gggatgctca tcacgcggta
     1321 ctcctgcgcc acgcgcatgt tgtcgtcgac gttgagcttg ccgaccttga ccttgccggc
     1381 gtagtcggaa gcgagctcct cgatgacggg tccgaccatg cggcaggggc cgcaccaggg
     1441 ggccca
//
LOCUS       DAOQYE010000675         1444 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_113037_length_1444_cov_1.904968, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000675 DAOQYE010000000
VERSION     DAOQYE010000675.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1444
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113037_length_1444_cov_1.904968"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1444
                     /locus_tag="PKN52_10090"
     CDS             <1..>1444
                     /locus_tag="PKN52_10090"
                     /inference="COORDINATES: protein motif:HMM:NF024821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNR00342.1"
                     /translation="HHPPQPTDAVTLERELLESALSIAPNDIDVLARLASVNVNLRNY
                     QVAFDLLNRALDLRPDIEAIKDMRDTVASLVDASKETDPVKRALAEAQQAQDKGQLDQ
                     AIDIIRKGLATNPDDVRLISGLAGMLYEAKRWDELRETVARGLAIDPENRNLQTMQTL
                     ADLGDDVEARIAAVENKADIPELDRQIALHQLYLASKDPEAAKAALAAARAIDPQDRR
                     VVIHSFDEAIRARDAAEAERIYQANKDRDIDGADGLAMRARVELAKGDKESAKRTLEA
                     AVTRGSLNPVTMRLLADVQLDMGETFKALDNYKRAIAADPSDVDLLKAYIVVLVRLDR
                     VSEALDTARTSLAIAQRDEQFREMWLSLEGQVGNKQLAYDRRIEIAEASPDDANNSAQ
                     LIALALDLRKFDEARQRLDAARAKKDSLLLASLDARYHADRSDMNAAMNAYTSFMASP
                     ANDLSRPDAYLAFGQFLIERGQIDRGLTTLR"
BASE COUNT          286 a          534 c          424 g          200 t
ORIGIN      
        1 tcatcatccc ccacagccaa ctgatgcggt aacgctcgag cgcgagctcc tcgagagcgc
       61 gctctccatc gcccccaacg acatcgacgt cctggcgcgc ctggcaagtg tgaacgtcaa
      121 ccttcgcaac taccaagtgg ccttcgacct gctcaaccgg gcactcgacc tccgccctga
      181 tatcgaggcc atcaaggaca tgcgcgacac ggtcgcttcg ctcgtggacg cctcgaagga
      241 aacggacccc gtcaagcgcg ctctcgcgga ggcgcagcaa gcccaggata agggccagct
      301 cgatcaggcc atcgatatca tccgcaaggg tctcgccacg aaccccgacg acgtgcgtct
      361 gatctccggg ctcgccggga tgctctacga ggcgaagcgg tgggacgaac tccgcgagac
      421 cgtcgctcgc gggctcgcga tcgaccccga gaaccgcaac ctacagacca tgcaaacgct
      481 cgcggacctt ggcgacgacg ttgaggcccg gattgccgcc gtggagaaca aggcggacat
      541 ccccgagctc gatcggcaga tcgcgcttca ccagctctat ctcgcgagca aggatcccga
      601 agcagccaag gccgccctcg ccgctgcgcg ggccatcgac ccgcaggacc ggcgcgtggt
      661 catccactcc ttcgacgagg ccatccgcgc ccgcgacgcc gccgaggccg agcggatcta
      721 ccaggccaac aaggaccgcg acatcgacgg agccgatggc cttgcgatgc gagcccgggt
      781 cgagctcgcc aagggggaca aggaatccgc caagcggaca cttgaggccg ccgtcaccag
      841 gggcagcctc aacccagtca ccatgcgcct gctcgcggac gtgcagctag acatgggcga
      901 gaccttcaag gccctcgaca actacaagcg cgccatcgcc gctgacccat ccgacgtgga
      961 tcttctaaag gcgtacatcg tcgttctcgt ccgtctcgat cgcgtgagcg aggcgctcga
     1021 caccgcccgc acctcgctcg cgatcgctca gcgcgacgag cagttccgcg agatgtggct
     1081 cagcctcgag ggacaggtcg gcaacaagca gctcgcctac gaccgccgca tcgagatcgc
     1141 ggaggccagc cccgacgacg ccaacaactc ggcccagctc atcgcgctcg cgctcgacct
     1201 gcgcaagttc gacgaggccc gccagcggct cgacgcggcc cgtgcgaaga aagactcgct
     1261 cctgctcgcc tcactcgacg ctcgctacca cgccgaccgg agcgacatga acgccgcgat
     1321 gaacgcgtac acgtcgttca tggcctcccc cgccaacgat ctgagcaggc ccgacgccta
     1381 ccttgccttc ggccagttcc tgatcgagag ggggcagatc gaccgcggtc ttaccacact
     1441 cagg
//
LOCUS       DAOQYE010000676         1441 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_113406_length_1441_cov_2.430014, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000676 DAOQYE010000000
VERSION     DAOQYE010000676.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1441
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113406_length_1441_cov_2.430014"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..701)
                     /locus_tag="PKN52_10095"
     CDS             complement(<1..701)
                     /locus_tag="PKN52_10095"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNR00343.1"
                     /translation="MASKGAGERATAQHDIVIIGAGPGGYVAAIRGAQLGFDVAVVER
                     ESALGGTCVRVGCIPSKALLESSFKYVEAKDDLAEHGVIVDGVRADLAAMQARRAKVV
                     KQNTDGVEYLFKKNKITRYTGTGRLTGDRTVTVTAGDGGMTEIVAEHVVLATGSVTSQ
                     LRGVEVDGEVVVTSTEALEFAEVPSSLVVIGAGYIGLELGSVWARLGAQVTVLEYLER
                     ILPGMDLEIAREALRV"
     gene            complement(703..>1441)
                     /gene="sucB"
                     /locus_tag="PKN52_10100"
     CDS             complement(703..>1441)
                     /gene="sucB"
                     /locus_tag="PKN52_10100"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006554 - lysine catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HNR00344.1"
                     /translation="AEPERPLGAGGYEREEEIKPMSLLRRRIAERLVSAQQTAALLTT
                     FNEVDMSAVMQLRSEYKDLFEKRYGVRLGFMSFFVKAAIDALKTVPQLNAEIRGTDVV
                     YKNYYDIGVAVSTGRGLVVPVIRDAERLSFAEVEQAIADFGTRANANKLAPEELQGGT
                     FTISNGGVFGSLLSTPIVNPPQSGVLGMHSIQERPVAVNGQVVIRPMMYLALTYDHRM
                     VDGREAVTFLKRIKDTVEKPARMLLEI"
BASE COUNT          208 a          499 c          491 g          243 t
ORIGIN      
        1 acgcgcaggg cctcgcgcgc gatctccagg tccatgcccg ggaggatgcg ctccaggtac
       61 tcgagcacgg tcacctgcgc gccgagccgc gcccacacgg agccgagctc cagaccgatg
      121 tagccggcgc cgatcacgac gaggctgctc ggcacctcgg cgaactcgag cgcttccgtg
      181 ctggtgacga cgacctcgcc gtcgacttcc acgccccgca gttggctcgt caccgagccc
      241 gtggccagca cgacgtgctc ggccacgatc tccgtcatgc cgccatcgcc ggcggtcacc
      301 gtcacggtgc ggtcgcccgt tagcctgccc gtgcccgtgt accgcgtgat cttgttcttc
      361 ttgaacaggt actcgacgcc gtcggtgttc tgcttgacga ccttggccct gcgggcctgc
      421 atggccgcca ggtcggcgcg cacgccgtcg acgatcacgc cgtgctcggc caagtcgtcc
      481 ttggcctcaa cgtacttgaa gctggactcg aggagcgcct tgctcggtat gcagcctacc
      541 cgcacgcacg tcccgcccag ggcggactcg cgctcgacca cggccacgtc gaaaccgagc
      601 tgcgcgccgc ggatggcggc cacgtaaccg ccaggccccg cgccgatgat cacgatgtcg
      661 tgctgggcgg tggccctctc ccctgctccc ttggacgcca tctcagatct ccaggagcat
      721 ccgggccggc ttctcgacgg tgtccttgat gcgcttgagg aaggtgacgg cctcgcgccc
      781 gtcgaccatg cggtggtcgt acgtgagggc gaggtacatc atggggcgga tcacgacctg
      841 gccgttcacg gccaccgggc gctcctggat ggagtgcatg ccgagcacgc ccgattgggg
      901 cgggttgacg atcggcgtgg agaggagcga gccgaagacc ccgccgttgc tgatggtgaa
      961 ggtgccgccc tgcagttcct cgggcgcgag cttgttggcg ttggcccgcg tgccgaagtc
     1021 cgcgatggcc tgctcgacct cggcgaacga caggcgctcc gcgtcgcgga tgacggggac
     1081 cacgagccca cggcccgtcg agacggccac gccgatgtcg tagtagttct tgtagacgac
     1141 gtcggtgccg cggatctcgg cgttgagctg gggcacggtc ttgagcgcgt cgatggccgc
     1201 cttgacgaag aacgacatga agccgaggcg cacgccgtag cgcttctcga agaggtcctt
     1261 gtactcgctg cgcagctgca tgacggcgct catgtcgacc tcgttgaagg tcgtgaggag
     1321 ggcggccgtc tgctgggccg aaacgaggcg ctccgcgatg cggcgccgga ggaggctcat
     1381 cggcttgatc tcctcctcac gctcgtaacc gccggcgcca aggggccgct cgggctccgc
     1441 g
//
LOCUS       DAOQYE010000677         1437 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_113932_length_1437_cov_2.217800, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000677 DAOQYE010000000
VERSION     DAOQYE010000677.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1437
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113932_length_1437_cov_2.217800"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1071
                     /locus_tag="PKN52_10105"
     CDS             <1..1071
                     /locus_tag="PKN52_10105"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNR00345.1"
                     /translation="AERAAERLLAVNGAAALVGGLGDGQADVLSNVAEGAGVPFLNIG
                     SPASALRTGCLRSTFNIEAGAGMYLGAMASWFTDHGHERWFVVYEETGEGTELRDAAL
                     AALAATPDAVLAGSTGVMTGQPDYGPVLEEVRRSGADLILVLVNAPDQIAFLAQEESV
                     GPHVAALPYPFPVTQTRDFLGAMRVRADAQGLGFRVALWDATLGGGDGADDGGPTGRS
                     GSAAAGDALNDRFVARWGQPMDPAAWAAYAAITILHDAAAAAGSTLPEAIAAYLASPE
                     ARFEVGKSTPLAFDPSSRQLQQPLYVIELDPDALLTGKLNDQRGLARLVAVVDGAVQA
                     GVMDADGSEGGGAPLDTSRCQR"
BASE COUNT          198 a          466 c          553 g          220 t
ORIGIN      
        1 gccgagcggg ccgcggagcg gctcctggcc gtcaacggcg ccgccgcgct cgtcgggggc
       61 ctgggcgacg ggcaggcgga cgtgctctcc aacgtggcgg aaggcgcggg cgtgcccttc
      121 ctcaacatcg gctcccccgc gagcgcgctg aggaccgggt gcctcaggag cacgttcaac
      181 atcgaggccg gcgcgggcat gtacttgggc gccatggcga gttggttcac ggaccacggg
      241 cacgagcggt ggttcgtcgt gtacgaggag acgggggagg gcaccgagct gcgcgacgcg
      301 gcgctggctg ccctcgcggc cacccccgac gcggttctcg ctggctcgac gggtgtgatg
      361 acgggccagc cggactacgg cccggtgctc gaggaggtgc gtcgcagcgg cgccgacctg
      421 atcctggtgc tcgtgaacgc gcccgatcag atcgcgttcc tggcgcagga ggagtccgtc
      481 gggccgcacg tggcggcgct cccgtacccg ttccccgtca cgcagacgcg tgacttcctg
      541 ggggcgatgc gcgtgcgggc ggacgcgcag ggcctcgggt tccgggtggc gctatgggac
      601 gcgaccctcg gcgggggtga cggcgcggac gacggcgggc ccaccggccg gtctggcagc
      661 gcggctgccg gcgacgcgct caacgaccgc ttcgtggcgc gctggggtca gccgatggac
      721 ccggcagcgt gggcggccta cgccgccatc acgatcttgc acgacgccgc ggccgcggcc
      781 ggctcgacgc tcccggaggc catcgccgcg tacctggcga gcccggaggc gcggttcgag
      841 gtcgggaagt cgacgccctt ggcgttcgac ccgtccagcc ggcagctgca acagcccctt
      901 tacgtgatcg agctggaccc cgacgcgtta ctcacgggga agttgaacga tcagcgtggg
      961 cttgcgcggc tggtggctgt cgtggatggc gccgtacaag cgggggtgat ggatgctgac
     1021 ggtagtgaag ggggcggtgc gccgttggac acttcgcggt gtcagcgatg acggtccact
     1081 agtaaccggt cagattggtg gccgttcgag cggacgagga acgtttctga ccggtttccg
     1141 ccccgagcgc tccgactcga gcggtcgcgc caacggaagc tgggagttcc cgttcccgac
     1201 gtagaggcta tagagacgta caggcaaagg aaagccccga gcatcttcgc ggctgcgcgc
     1261 tggagaggac gggttcgggg tctatggcga aagcctagga cgctcttgcg tcgcggtcaa
     1321 gtcgatcagg cgcactcatc gcgcccgatc ttgaagcggc gctgtcgcgt tctcgcctgg
     1381 ccccgtaccc tggtgtgacg cccgacccac ggaggtcgcc ggggcttgtg gacctgg
//
LOCUS       DAOQYE010000678         1435 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_114166_length_1435_cov_3.047101, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000678 DAOQYE010000000
VERSION     DAOQYE010000678.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1435
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114166_length_1435_cov_3.047101"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(157..>1435)
                     /locus_tag="PKN52_10110"
     CDS             complement(157..>1435)
                     /locus_tag="PKN52_10110"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030729 - acetoacetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HNR00346.1"
                     /translation="PYPGEELRLAGAPTWRAWLEPHRGREAPDYSALPFDHPLYVLYS
                     SGTTGLPKAMVHRAGGALLTHRKEHALHSDIRPGDVVFYYTTTGWMMWNWLVSALASG
                     ATIVLYDGSPAYPDTLATFALAERLGVTFFGTSARFLQELAGAGARPGRERDLGTIRT
                     IASTGSPLSPRGFEYVYDAVKADVHLASISGGTDIVGCFMLGVPTLPVFAGEIQRPGL
                     GVDLRVLDASGAEVRQVHGELVCASPLPSMPLRFVGDDDFARYASAYFSTYAGVWRHG
                     DLVERTVNGGIIVYGRSDATLNPGGVRIGTAEIYRPLEGVGEVREAVAVAKRVGTDGE
                     DQEIWLLVVLREGERLDARLEARIKRAVREGASPRHVPRRVLDVPELPRTRSGKVMEL
                     AVTHLVNGESVGNREVVANPGSLDAIARRLSAG"
BASE COUNT          215 a          505 c          515 g          200 t
ORIGIN      
        1 cgtcgccatc gcgctgttca cgggcgagat cgcccgccag gagccgttcg gcgagatcat
       61 ggccgccgcc gtgctcgtca cgatcccgtt ggtggtcctc gtgctcgtgt tccagcagcg
      121 catcgtggcc gggctcacgg ccggggccgt caagggctag ccggcgctca aacgcctcgc
      181 gatggcgtcg agcgacccgg ggttggccac cacctcgcgg ttgccgacgc tctcgccgtt
      241 gacgagatgc gtaaccgcca gctccatgac tttgccagag cgcgtgcgag gcagctcggg
      301 cacgtcgagg acgcgccttg gcacgtggcg gggcgacgcg ccctcgcgga ccgcacgctt
      361 gatgcgcgcc tcgagccggg cgtccagcct ctcgccttcc cggagcacga cgagcagcca
      421 gatctcctga tcctcgccgt cggtccccac gcgcttcgcg acagcgacgg cctcgcgcac
      481 ctcaccgacc ccctcgagcg ggcggtagat ctccgccgtg cctatccgca cgccgccagg
      541 gttcagggtg gcgtcagacc ggccgtacac gatgatcccg ccgttgacgg tgcgctccac
      601 caggtcgccg tgacgccata cccctgcgta cgtcgagaag taggcagagg catagcgggc
      661 gaagtcgtcg tcacccacga agcggagcgg catcgagggg agcggcgagg cgcacacgag
      721 ctcgccatgg acctggcgca cttcggcgcc gctcgcgtcg aggactcgca gatcgacgcc
      781 aagcccgggt cgctggatct cgccggcgaa cacgggcagc gtcggcacgc cgagcatgaa
      841 gcagcccacg atgtcggtgc cgccggagat gctcgcaagg tgaacgtccg ccttgacggc
      901 gtcgtaaacg tactcgaagc cgcgcggtga cagcggcgag cccgtcgacg ctatggtcct
      961 gatggtgcca aggtcgcgct cgcgccccgg acgcgctccc gcgccggcga gctcctgcag
     1021 gaagcgcgcg ctcgtgccga agaacgtcac gccgagccgc tcggccaatg cgaacgtggc
     1081 cagcgtgtcg gggtaggcgg gcgagccgtc gtacaggacg atggtggcgc ccgacgccag
     1141 ggcggagacg agccagttcc acatcatcca gccggtcgtc gtgtagtaga agacgacgtc
     1201 gcccgggcgg atgtcggagt gcagcgcgtg ctccttgcgg tgggtgagga gcgcaccgcc
     1261 cgcccggtgc accatggcct tcggcaggcc ggtcgtgccg gagctgtaga gcacgtacag
     1321 ggggtggtcg aacggcaggg cggagtagtc cggggcctcg cgcccgcggt gcggctccag
     1381 ccaggctcgc caggtcgggg ccccggcgag acgcagctcc tcgcccgggt acggc
//
LOCUS       DAOQYE010000679         1434 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_114290_length_1434_cov_3.285714, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000679 DAOQYE010000000
VERSION     DAOQYE010000679.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1434
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114290_length_1434_cov_3.285714"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /locus_tag="PKN52_10115"
     CDS             <1..667
                     /locus_tag="PKN52_10115"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNR00347.1"
                     /translation="TFYPYLSGRANLRHAALLHGDVPDGRVDEALAFVRMQDAASKKV
                     KAYSQGMRQRLALARALLWRPQVLLLDEPTNGLDPTGIAEFRESLKEVAAEGTTVLVS
                     SHILAEVEKFVDRVLAIDKGVLRFDGRLGDLLARMGEQQVRYTVRAADQAALFAALER
                     LGLRHTPNGHDGADVMVARDRSDDLVGDLVAQGVRVLAASVHGDDLEAAYLKLVNGQG
                     RPS"
     gene            664..>1434
                     /locus_tag="PKN52_10120"
     CDS             664..>1434
                     /locus_tag="PKN52_10120"
                     /inference="COORDINATES: protein motif:HMM:NF024141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNR00348.1"
                     /translation="MNALANVIRAETFKVLRKRRLYILAALYWFLLPVLVLVVARVVH
                     VNLAGSFANDGGTVDQGLQLFASPVGIARLALAGPAFTSPTLYIVAVTLLAALLIGDE
                     RSQNMWKTVLVVQPKRWAVLAGKVVVTMGALAALMLGAAVFGFLFGAIGTTFLPTDFS
                     GAWPPLIGLYLLQLAQLLAPVLLAFLLITLVRSGVLGVVMVLFLPSLIETLYTVLNTI
                     AQLQPLNRINAIFQALRIQNTWQALPKYFFTSNLYNPGR"
BASE COUNT          197 a          532 c          457 g          248 t
ORIGIN      
        1 gaccttctac ccgtacctgt cggggcgcgc caacctgcgc catgccgcct tgctgcacgg
       61 cgacgtgccc gatgggcgtg tcgacgaggc gctcgccttc gtccgcatgc aggacgccgc
      121 ctccaagaag gtcaaggcct actcgcaggg catgcgccaa cgcctggcgc tcgcccgtgc
      181 gctcctctgg cgcccccagg tcctgctcct cgacgagccg accaacgggc tcgacccgac
      241 gggcatcgcc gagttccgcg agtccctcaa ggaggtggcg gccgaaggga ccacggtgct
      301 cgtctcgagc cacatcctcg ccgaggtcga gaagttcgtc gaccgcgtcc tcgccatcga
      361 caagggcgtc ctgaggttcg acggcaggct cggcgacctg ttggcgcgga tgggcgagca
      421 gcaggtgcgc tacacggtac gcgcggccga ccaggcggct ctcttcgccg ccttggagcg
      481 cctcggcctt cgtcacaccc ccaacggcca cgacggcgcc gacgtcatgg tcgcccgcga
      541 tcgctccgac gacctcgtcg gcgacctggt ggcccagggc gtcagggtgc tcgccgcgag
      601 cgtgcacggt gatgacctcg aggccgccta tctcaagctc gtcaacggtc aagggaggcc
      661 atcgtgaacg ctctggccaa cgtcatccgg gcggagacgt tcaaggtgct gcgcaagcgg
      721 cgcctgtaca tcctcgccgc cctctactgg ttcctgctgc cggtgctcgt cctggtcgtg
      781 gcgcgcgtcg tccacgtgaa cctcgccggc tccttcgcca acgacggtgg cacggtggac
      841 caggggctcc agctcttcgc gtcgcccgtc ggcatcgcgc gcctcgccct cgccgggccg
      901 gcgttcacca gcccgaccct gtacatcgtg gccgtcacgc tcctggccgc gctgctgatc
      961 ggtgacgagc gctcgcagaa catgtggaag accgtgctcg tcgtgcagcc caagcgctgg
     1021 gccgtgctcg ccggcaaggt cgtcgtgacc atgggggcgc tcgccgccct gatgctcgga
     1081 gccgccgtgt tcggcttcct cttcggggcc atcggcacga cgttcctgcc gaccgacttc
     1141 agcggcgcct ggccgcccct catcgggctc tacctcctgc agttggccca gttgctcgcg
     1201 ccggtgctgc tcgcgttcct gctcatcacg ctcgtgcgtt cgggggtgct cggggtggtg
     1261 atggtcctct tcctcccgag cctgatcgag acgctctaca cggtgctgaa cacgatcgcc
     1321 cagctccagc cgctgaacag gatcaacgcc atcttccagg cgctgcgcat ccagaacacc
     1381 tggcaggcgc tgcccaagta cttcttcacg agtaacctgt acaaccccgg cagg
//
LOCUS       DAOQYE010000680         1433 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_114426_length_1433_cov_3.379536, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000680 DAOQYE010000000
VERSION     DAOQYE010000680.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1433
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114426_length_1433_cov_3.379536"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1317)
                     /locus_tag="PKN52_10125"
     CDS             complement(<1..1317)
                     /locus_tag="PKN52_10125"
                     /inference="COORDINATES: protein motif:HMM:NF013079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family peptidase N-terminal
                     domain-containing protein"
                     /protein_id="HNR00349.1"
                     /translation="MTNRFDDRGAPRLGFPTLPTSEVLGGQIQDTAVDAIVVGLLAGV
                     TSPGGAAGAVDAAMGGAISRLIAAGDLTGKLGEVTVLYPASGVVAPRVLVVGLGSKDA
                     FGLEAVRRASGTAAARARELGATTLASAVHGAGVGGLATSEAAQATLEGALLASYRFG
                     GWHARGSGQERALSKVALVELDGNRLPQVREGATAAAAIARGVALARDLVNLPPVYAT
                     PVYLADAAAAMAERTGLRVTIGDRAWAEERAMGAFLAVTKGSALEPRFIELGHNEHRT
                     DLPLVVLVGKGVTFDSGGLSLKTRDGMVPMKSDMSGAAAVIGAMQVVAELGLNARVIG
                     LAPCVENMPDGAAYRPSDVLVAGNGLSIEVISTDAEGRLALADALVHAKGYAPAAVID
                     IATLTGASVTALGEGVAASLFFNDPTLRARLETAAEIRRLQRDGCDV"
BASE COUNT          195 a          525 c          516 g          197 t
ORIGIN      
        1 cacgtcgcag ccatcgcgct gcagtcggcg aatctccgcc gcggtctcga gacgggcccg
       61 caacgtcggg tcgttgaaga agagcgaggc ggccacgccc tcgcctagcg ccgtgacgct
      121 ggccccggtg agcgttgcga tgtcgatgac ggccgcgggc gcgtagccct tggcgtgcac
      181 gagcgcgtcg gcgagggcga ggcgcccctc ggcgtcggtc gagatgacct cgatggagag
      241 cccgttgccc gccacgagca cgtcggatgg tcggtacgct gcgccgtccg gcatgttctc
      301 gacgcagggg gccaagccga tgacacgcgc gttcagcccg agttcggcca ccacctgcat
      361 ggcgccgatg accgcggcgg cgcccgacat gtccgacttc atcgggacca tgccgtcgcg
      421 cgtcttgagc gagaggccgc cggagtcgaa cgtgacgccc ttgccgacga ggacgacgag
      481 gggcaggtcc gtgcggtgct cgttgtgccc gagctcgatg aaccgcggtt cgagggcgct
      541 gcccttggta acggccagga acgcgcccat ggcgcgctcc tcggcccatg cgcggtcacc
      601 gatcgtcacg cgcaggccgg tgcgctcggc catggcggcc gccgcgtcgg ccaggtacac
      661 gggggtcgcg tacacgggcg gcaggttgac caggtcgcgc gctagagcga cgccgcgggc
      721 gatcgcggcc gcggccgtcg cgccttcgcg gacctgcggc aggcggttgc cgtccagctc
      781 gacgagggcg accttcgaca gcgcgcgctc ctgtccggag ccccgcgcgt gccacccgcc
      841 gaagcggtaa ctggcgagca gcgcgccctc caacgtggct tgagccgctt cgctcgtggc
      901 caggccgccg acgccggcgc cgtggaccgc cgaggccagc gtagtggcgc ccaactcgcg
      961 cgcccgcgcc gccgcggtcc cggaagcgcg ccgcaccgcc tcgaggccga aggcatcctt
     1021 gcttcccaac cccacgacga ggacgcgcgg cgcgacgacc ccgcttgccg gatagaggac
     1081 cgtcacctcg ccgagcttgc cggtcaggtc gccggccgcg atcaggcgcg agatcgcgcc
     1141 gcccatggcc gcgtctacgg cgcccgcggc accgcctggg ctcgtcaccc cggcgaggag
     1201 cccgaccacg atggcgtcca cggcggtgtc ctggatctga ccgccgagca cctcgctggt
     1261 cggaagcgtc gggaagccga ggcgcggcgc gccccggtcg tcgaagcggt tcgtcatgtt
     1321 ccctcctggg ggcgctttcg cgccgatgcc ggccatgcta acgccgttgg tcgtcgaccg
     1381 tttgcaatct catcggcgtc cgtctataga cggacgccga tgagattgca aac
//
LOCUS       DAOQYE010000681         1433 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_114487_length_1433_cov_2.275762, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000681 DAOQYE010000000
VERSION     DAOQYE010000681.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1433
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114487_length_1433_cov_2.275762"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(70..489)
                     /locus_tag="PKN52_10130"
     CDS             complement(70..489)
                     /locus_tag="PKN52_10130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00350.1"
                     /translation="MRGFIGFIVVLVIVFFAVGETRGWYLGIPTQTPVFVYKKDFTST
                     TSRRTTQRTDMPVRFTGDVKRGSVTIEVRYQRPTSFQTSQAAGPETKVYEVTYSAGQR
                     AALDRVFENGGGVYTVLVRYQDATGLFTLTLPGGTDL"
     gene            complement(567..>1433)
                     /locus_tag="PKN52_10135"
     CDS             complement(567..>1433)
                     /locus_tag="PKN52_10135"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176669.1"
                     /note="Catalyzes the conversion of citrate to isocitrate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HNR00351.1"
                     /translation="DGIERSNEAWNAIPVKGGQLFDWDDSSTYIQEPPFFIDMPEHPT
                     GIAPIRGARALVKVGDSVTTDHISPAGSIALNSPAGKFLVEHGVTKADFNSYGSRRGN
                     DRVMTRGTFANIRLKNQLASGTEGGVTTDFTTGEVASIYDASRNYIAMGIPTVVLAGN
                     DYGMGSSRDWAAKGTYLLGVKAVIAKSYERIHRSNLVGMGVLPLQFLDGQDPDTLGLD
                     GSETFDIGIDESVAPRARLKVTATKADGRRVELEVACRLDTPVEVEYYRNGGILHTVL
                     RNLHRSATRATA"
BASE COUNT          208 a          464 c          522 g          239 t
ORIGIN      
        1 gacggtcgcc tgggagcgga cggccggctc aggcgcgtgt tcgcccggcg cggcgagagc
       61 cggccgaccc tagaggtcgg tgccccccgg caacgtgagg gtgaacaggc ccgtcgcgtc
      121 ctggtagcgg accaagacgg tgtacacacc gccgccgttc tcgaacacgc ggtcgagcgc
      181 cgcccgctgg ccggctgagt aggtcacctc gtagaccttc gtctccggac cggcggcctg
      241 cgaggtctgg aagctggtcg ggcgctggta gcgcacctcg atggtcacgg agccgcgctt
      301 cacgtcgccc gtgaagcgta ccggcatgtc ggtgcgctgc gtcgtcctgc gggacgtcgt
      361 ggacgtgaag tccttcttgt acacgaacac tggcgtctgg gtcgggatgc cgaggtacca
      421 gccccgcgtc tcacctacgg cgaagaagac gatgaccagc acgacgatga acccgatgaa
      481 accgcgcatg gcgagagtat accggcaagc ggcggcggcg ctgcgggctt ctgcccaggc
      541 gccgccgccc cagacggggg ccgccgctag gcggtcgcgc gcgtggcgga gcggtgcagg
      601 ttacgcagca ccgtgtgcag gatgccgccg ttgcggtagt actcgacctc caccggcgtg
      661 tcgagccggc aggccacctc gagctcgacc ctgcggccgt ccgccttcgt ggccgtgacc
      721 ttcagcctgg cccgaggcgc cacgctctcg tctatgccga tgtcgaacgt ctcgctgccg
      781 tccagcccga gcgtatcggg gtcctgacca tcgaggaact ggagcgggag aacgcccatg
      841 ccgacgaggt tggagcggtg gatgcgctcg tagctcttcg ctatgaccgc cttgacgccg
      901 agcaggtagg tgcccttggc cgcccagtcg cggctcgagc ccatgccgta gtcgttgccg
      961 gccaggacga cggtcggtat gcccatggcg atgtagttgc gactggcgtc gtagatgctg
     1021 gcgacctcgc cggtcgtgaa gtcggtcgtc acgccgccct cggtgccgga ggcgagctgg
     1081 ttcttgaggc ggatgttggc gaacgtgccg cgcgtcatga ccctgtcgtt gccgcgccgc
     1141 gagccgtacg agttgaaatc ggccttggtc acgccgtgct cgacgaggaa cttgccggcg
     1201 gggctgttga gcgctatcga gccggccggc gagatgtggt cggtcgtcac ggagtcgccg
     1261 accttgacga gcgcgcgggc accccggatg ggggcgatgc ccgtggggtg ctcgggcatg
     1321 tcgatgaaga acggcggctc ctggatgtag gtcgaggaat cgtcccagtc gaagagctgg
     1381 ccacccttca cggggatggc gttccacgcc tcgttggagc gctcgatgcc gtc
//
LOCUS       DAOQYE010000682         1431 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_114747_length_1431_cov_2.486192, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000682 DAOQYE010000000
VERSION     DAOQYE010000682.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1431
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114747_length_1431_cov_2.486192"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..869)
                     /locus_tag="PKN52_10140"
     CDS             complement(<1..869)
                     /locus_tag="PKN52_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNR00352.1"
                     /translation="MLPYILRRVLTAVPTLLIVTVMVFAVQRMLPGDPAVVLAGEERD
                     PEVIAFIRDRYRLNDPVPVQYVAWLGQVTQGNLGQSFRTRQPVAQLLLEKLPVTVELA
                     ILSLLVALAIAIPTGVLAAVRKGTAVDYASTVAALSGISIPNFWLGIMLILLVSVRWK
                     LLPASGFVPLSDGVWPNLQRMIMPALVLGTGLAGVLMRQMRSSMLEVLKQDFVRTARA
                     KGLAPFLVVVRHALRNALIPVITIIGLQLGALLSGAVLTEQVFTIPGFGKLVIDFDPA
                     ALGQAVDRHAELP"
     gene            complement(899..>1431)
                     /locus_tag="PKN52_10145"
     CDS             complement(899..>1431)
                     /locus_tag="PKN52_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNR00353.1"
                     /translation="PIPERDVDRAKALIAEAGYTRVPVELIVANSPDSVRVGEVVQAM
                     AGEAGFDVSLRATEFATALDLQDAGDYDAFAVGWSGRLDPDGNIHSFVTCEGALNVSG
                     YCDPEVDEWMNESRAVSDFAARKALFDKAAEHYIPDRHIIYLYHTQLFFPHTTRLHGF
                     VAFPDGMIRLSGVSLE"
BASE COUNT          242 a          457 c          536 g          196 t
ORIGIN      
        1 atcggcagct cggcgtgacg gtccaccgcc tgcccgagcg ccgcggggtc gaagtcgatg
       61 acgagcttcc cgaagccggg gatggtgaac acctgctccg tgagcaccgc gccggagagg
      121 agcgccccga gctgcaggcc gatgatcgtg atgacgggga tgagggcgtt gcgcagcgcg
      181 tgcctcacga cgacgaggaa cggcgcgagg cccttggcgc gcgccgtgcg gacgaagtcc
      241 tgcttcagga cctccagcat ggaggagcgc atctggcgca tcagcacccc ggccaggccc
      301 gtgccgagca cgagcgcggg catgatcatg cgctggaggt taggccacac gccgtcggag
      361 aggggcacga agcccgacgc aggcaggagc ttccagcgca cggacacgag caggatgagc
      421 atgatgccga gccagaagtt ggggatggag atgccggaga gcgccgccac ggtgctcgcg
      481 tagtcgaccg ccgtgccttt gcgcacggcg gccaggaccc cggtggggat ggcgatggcg
      541 agcgccacga ggaggctcag gatcgccagc tcgacggtga cgggcagctt ctcgaggagg
      601 agctgcgcca cgggttgccg cgtccggaac gactggccca ggttcccctg cgtcacctga
      661 ccaagccagg cgacgtactg gacgggcacg gggtcgttga gccggtagcg gtccctgatg
      721 aaggcgatga cctccgggtc acgctcctca ccggcaagca ccacggcggg gtcgcccggc
      781 agcatgcgct ggacggcgaa caccatgacg gtgacgatga ggagcgtggg gaccgcggtc
      841 aggacgcggc gcaggatgta aggcagcacc ctacggcccc gccccggtca gtctcttgtc
      901 actcgagcga caccccgctc aggcggatca tcccgtccgg gaacgcgacg aagccgtgca
      961 gcctcgtggt gtgcgggaag aacagctgcg tgtgatagag gtagatgatg tgccgatcgg
     1021 ggatgtagtg ctcggcggcc ttgtcgaaca gcgccttgcg ggcggcgaag tcgctgaccg
     1081 cgcgggactc gttcatccat tcgtccactt cggggtcgca gtagccggac acgttgagcg
     1141 cgccctcgca cgtgacgaag gagtggatgt tcccgtcggg gtcgagcctg cccgaccagc
     1201 cgacggcgaa ggcgtcgtag tcgcccgcgt cctgcaggtc gagggccgtg gcgaactcgg
     1261 tcgcgcgcaa cgacacgtcg aagcctgcct cgccggccat ggcctgcacg acctcgccga
     1321 cgcgcacgga gtcggggctg ttcgcgacga tcagctcgac gggcacgcgc gtgtagccgg
     1381 cctcggcgat gagcgccttg gcgcggtcga cgtcgcgctc ggggatgggc a
//
LOCUS       DAOQYE010000683         1431 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_114797_length_1431_cov_1.771076, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000683 DAOQYE010000000
VERSION     DAOQYE010000683.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1431
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114797_length_1431_cov_1.771076"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1084
                     /locus_tag="PKN52_10150"
     CDS             <1..1084
                     /locus_tag="PKN52_10150"
                     /inference="COORDINATES: protein motif:HMM:NF027579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4910 domain-containing protein"
                     /protein_id="HNR00354.1"
                     /translation="VGPDGTRVVDHADSNLHLLGYSTAFRGRLTRAELDRHLYSLPDR
                     PDAVPYATSYYSPRWGFCLTQRRRDALPEGEYEVVVDTETFRGSMTLSDCLLPGASGR
                     EVLLSTYTCHPSLANNELSGPLVAAALYARLAAWPTRRLGYRFVLGPETIGALAYLSL
                     HGEHLVAHVDAGLVLTCLGDRAPLTYKRSRQGDALGDRAARVALPAFGGTRWRDFSPL
                     GSDERQYSSPGFALPVGSLMRSAYGTYPEYHTSDDDKAFISFERLQESVDACEAWCRA
                     VDIGGASYRSLAPYGEPQLGRRGLYPDLTVGASNVGAQEAIFWVLNFADGEHDLLAVH
                     ERSGIALGELHAAAARCVGAGLIEAA"
     gene            complement(1183..>1431)
                     /locus_tag="PKN52_10155"
     CDS             complement(1183..>1431)
                     /locus_tag="PKN52_10155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00355.1"
                     /translation="ETGAGSARVDVAANHAIFGPQPFFAVRTWGGGYAFDNLDVQEPF
                     RAWSYVFDEQTFPPRAVEAFGFGAADAYGQTVAVRATL"
BASE COUNT          179 a          552 c          502 g          198 t
ORIGIN      
        1 cgtcgggccg gacggcacca gggtcgtcga tcacgccgac tcgaacctgc acctgctcgg
       61 ctactcgacg gcgttcaggg gcaggttgac gcgcgcggag ctcgaccgcc acctctactc
      121 gctgcccgat cgcccggacg ccgtccccta cgccacctcc tactactcgc cgcgctgggg
      181 cttctgcctc acgcagcggc gccgcgacgc cctgcctgaa ggggagtacg aggtggtcgt
      241 cgacacggag acgttccgcg ggtccatgac gctgtccgac tgcctgctgc cgggcgcgtc
      301 agggcgggag gtgctcctgt cgacgtacac ctgccacccg tcgctcgcca acaacgagct
      361 ctccggaccg ctcgtggccg cggccctgta cgcgcggctg gccgcctggc ccacccgccg
      421 cctcggctac cgcttcgtgc tcggccccga gacgataggc gcgctggcct acctgagcct
      481 gcacggcgag cacctcgtgg cgcacgtcga cgcgggcctc gtcctcacct gcctcggcga
      541 ccgcgcgccc ctgacctaca agcgctcgcg gcagggcgac gccctcggcg accgcgcggc
      601 gcgggtcgcg ctgcccgcct tcggcggaac caggtggcgc gacttctcac cgctcggctc
      661 cgacgaacgc cagtactcct cccccggctt cgcgctgccg gtcgggagcc tgatgcggag
      721 cgcgtacggc acctaccccg agtaccacac gtcggacgac gacaaggcct tcatctcgtt
      781 cgagcggctg caggagtcgg tggacgcctg cgaggcgtgg tgccgggccg tcgacatcgg
      841 cggcgccagc taccgcagcc tggcgcctta cggcgagccg caactggggc ggcgcggact
      901 ctacccggac cttacggtcg gcgcgagcaa cgtgggcgcc caggaggcca tcttctgggt
      961 gctcaacttc gccgacggcg agcacgacct gctggcggtg cacgagcgca gcggcatcgc
     1021 cctcggggag ctgcacgcgg cggcggcgcg ctgtgtaggc gccgggctca tcgaggccgc
     1081 ctgagagggt ccggcgtccg ccggggtccg gcgcccgccg gggtccggcg tccgccgggg
     1141 tccggcgtcc gccgggccgt cctcggtctg gcttcgtgcc cgtcagaggg tcgcgcgcac
     1201 cgccacggtc tggccgtagg cgtccgcggc gccgaacccg aacgcctcca ccgcgcgcgg
     1261 cgggaaggtc tgctcgtcga acacgtagct ccaggcacgg aacggctcct gcacgtcgag
     1321 gttgtcgaag gcgtagccgc cgccccacgt gcgcaccgcg aagaacggct gcggcccgaa
     1381 gatggcgtga ttggcggcca cgtcgacgcg cgccgatccc gccccggtct c
//
LOCUS       DAOQYE010000684         1428 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_115190_length_1428_cov_2.175528, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000684 DAOQYE010000000
VERSION     DAOQYE010000684.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1428
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115190_length_1428_cov_2.175528"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(330..962)
                     /locus_tag="PKN52_10160"
     CDS             complement(330..962)
                     /locus_tag="PKN52_10160"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNR00356.1"
                     /translation="MRETRFALESAAVELALELGVEQVTVEQIVEQANVSSRTFFNYF
                     AAKEDALLGHSLRDTDARFLASFPTESSGSSVYEDLKSFVVNRTSEQIFSDELLEKRM
                     RVLATSPNLAKRQMNQMEDFLDRLTVRVARRLAAEAGVADAEPGRELVAQANMLLRMC
                     AAVISYTLESWRREGGTSEPESAILGSFGLLERTIETQVRRQGVESVAGT"
     gene            1107..1373
                     /locus_tag="PKN52_10165"
     CDS             1107..1373
                     /locus_tag="PKN52_10165"
                     /inference="COORDINATES: protein motif:HMM:NF037716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclophilin-like fold protein"
                     /protein_id="HNR00357.1"
                     /translation="MRHTRDCAYRDCAYPDRAYPVERAVSGSPDGVDPDVGDITYYAP
                     WGNLAIFYRDFGYSRGLIKLGSFDGDIAILRQSGELSTTIELVE"
BASE COUNT          271 a          466 c          435 g          256 t
ORIGIN      
        1 ccggccaacc gccggccacc acgaacatgg cacccgcggt cgtcacgagc aacagcaaga
       61 tgaccttcgg cttggtgagc gtcaggtagt cgcgccaggt ggcccggggc ggagccggcg
      121 tcggttctaa ggggctgctg tcagtggtag cggggctcga agcgtctgtc acggtcggat
      181 gttctccaaa atggcgccga acttggcgac ttcatgggcc gctagacgcg acccctaagc
      241 gttgcgggac tgggcgacat aggccgggga tcgaggcgct caagaaccga gctgcgacct
      301 agggaagcag gagcggactc ggcgtcgccc tacgtcccgg ccactgactc aaccccttgc
      361 ctccggactt gcgtctctat ggtcctctca agcagcccga aggacccgag gatcgccgac
      421 tcgggttcac tcgtacctcc ttcacgacgc caagattcaa gcgtgtacga gatgacggcc
      481 gcacacatcc gaagcagcat gttcgcttga gcgaccagct cccgccccgg ctcggcgtcg
      541 gcaacaccgg cctcagccgc cagacgccgg gctacgcgca ccgtgagcct gtcgaggaag
      601 tcctccatct gattcatctg gcgcttcgca aggttggggc ttgtcgccag cacccgcata
      661 cgcttctcca acagctcatc ggagaagatc tgctcgctgg tccgattcac gacgaacgac
      721 ttcaggtcct cgtagacgct ggacccactc gattcggtgg ggaagcttgc caggaaccgc
      781 gcatcggtgt cccgcaagga gtgccccaac agcgcgtcct ctttggcggc gaaatagttg
      841 aagaaggtcc gggacgacac gttcgcctgc tctacgatct gttcgaccgt cacctgctcg
      901 accccaagtt cgagcgccag ctcgacggcg gcgctctcaa gcgcgaagcg cgtttcgcgc
      961 acctttctct ctcgcagacc cgggaggccc gacctatcca cgagcgatat agttgcatat
     1021 tctgcatgtt tacacaacgg gtaaaagtgt agaacgtgca ccttcgggtc gtcggcgaaa
     1081 cagccaccgg cccctcgcgg tagtccgtgc ggcacacgcg agactgcgcc tatcgagact
     1141 gcgcttatcc agaccgtgcc tatccggtcg agagggccgt ctcaggctcg cctgacggcg
     1201 tggatcccga tgtcggcgac atcacctact acgccccatg gggaaacttg gccatcttct
     1261 accgcgactt cggatactcc cggggcctga tcaagctggg gtccttcgac ggggacatcg
     1321 ctatcctgag acagtcggga gaactgagca cgaccatcga gctagtcgag taagaggcgt
     1381 cgaggaggta ggcccgcttc ctcagctgtg aaccgtgact ccgggggg
//
LOCUS       DAOQYE010000685         1425 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_115610_length_1425_cov_2.187591, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000685 DAOQYE010000000
VERSION     DAOQYE010000685.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115610_length_1425_cov_2.187591"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="PKN52_10170"
     CDS             complement(<1..346)
                     /locus_tag="PKN52_10170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00358.1"
                     /translation="MFDPAELFDTTRLPSELAELLIGGMVWETLARLDVFGAAIRNEL
                     RGSVHPTAIVDGRLTVMEGAEVGPHAYLTGPVYLFPGAKVGHGAYLRGPVVLGPGAHV
                     GHATEVKRSVLLG"
     gene            402..1295
                     /locus_tag="PKN52_10175"
     CDS             402..1295
                     /locus_tag="PKN52_10175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HNR00359.1"
                     /translation="MLQLVFNPAAGKGRAKAALATALAFFDERQVPYALHTTEGAGDA
                     TRIASATPPGSTVVAIGGDGTVHEVAKGLLQTGTADGRTAGRSLGILPLGSGDDFAFS
                     LGLARDDLRSALERLLTAEPRLVDFGTVNSEPFFNAVGTGLDAEVAERVGASPRFLKG
                     VSGYLYSVLGALVHLREPHARVLVDGALVHDGPCLLVTSQNGPRSGGSFVFALEARPD
                     DGMLDVVIAGKVGRLGALGLLPKVMQAKHLTHPAVQLRRGRNVRVEWSEPRPAHADGE
                     LLATDAAYEVALHPRALPVLA"
BASE COUNT          216 a          524 c          470 g          215 t
ORIGIN      
        1 cgccgaggag gaccgaccgc ttcacttccg tagcatgccc gacgtgcgca cccggaccca
       61 gcaccacggg tcctcggagg taggcgccgt ggcccacctt cgccccaggg aacaggtaga
      121 ccggccccgt caggtaggcg tgcgggccga cctcggcccc ctccatgacc gtcagccggc
      181 catccacgat ggccgtcggg tggacgctac cgcggagctc gttccggatg gccgctccga
      241 agacgtcaag gcgcgcgagg gtctcccaga ccatcccgcc gatcagtagt tcggccagct
      301 cggatggtag gcgcgtcgta tcgaagagct cggcggggtc gaacatgcgg cgagtctacc
      361 gccgttcgag ccctacacga cgtgtacaat cgtgcgcgta cgtgctgcaa ctcgtcttca
      421 accccgccgc gggcaaaggc cgcgccaagg ccgccctcgc gaccgccctg gccttcttcg
      481 acgagcggca ggttccgtac gcgctgcaca cgaccgaggg cgcaggggac gcgacgagga
      541 tcgcgtcggc cacgcctccc ggttcgaccg tcgtcgccat cggcggcgac ggaaccgtgc
      601 acgaggtggc caagggactg ttgcaaaccg gcaccgcgga cggtcgaacg gccggccggt
      661 cgctaggcat actgcccttg ggatcaggcg atgacttcgc cttcagcctc ggcttggcgc
      721 gcgacgacct tcggagcgca ctcgagcggc tgctgaccgc ggagccgcgg ctggtcgatt
      781 tcggaaccgt caacagtgag ccgttcttca acgccgtcgg gaccggcttg gacgccgagg
      841 tcgccgagcg cgtcggagcc tccccacgtt tcctcaaggg tgtctccggc tacctgtact
      901 cggtactcgg cgccttggtg cacttgcgcg agccacacgc gcgcgtgctc gtcgatggcg
      961 cgctcgtcca cgacgggccc tgcctactcg tgacctcgca gaacgggccc cgttcgggcg
     1021 gcagcttcgt gttcgcgctc gaagcccggc cggacgacgg catgctcgac gtcgtgatcg
     1081 cggggaaggt cggccgcctc ggcgccctcg ggctgctgcc caaggtcatg caggccaagc
     1141 accttaccca tcccgccgtg caactgcgcc gggggcgcaa cgtgcgcgtg gagtggtccg
     1201 aaccccgccc cgcccacgcc gatggcgagc tcctcgccac ggacgccgca tacgaggtgg
     1261 cgctgcaccc acgcgccctg cccgtgctcg cctgaccacc cgctcggccg ggaacgccaa
     1321 taccaacgaa agggaggccc cgcttgcgcg gggcctcctg ctactacttc cggctcacgc
     1381 cggtactgcc aaccgttcct tagaaggtga cggtgtagct gatcc
//
LOCUS       DAOQYE010000686         1420 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_116253_length_1420_cov_2.663004, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000686 DAOQYE010000000
VERSION     DAOQYE010000686.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1420
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116253_length_1420_cov_2.663004"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            203..1267
                     /locus_tag="PKN52_10180"
     CDS             203..1267
                     /locus_tag="PKN52_10180"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HNR00360.1"
                     /translation="MRRTIAACLVAVVVSAAAAQTDWTCPDGFAGQTLSVYNWATYVA
                     DDTISNFEAACGVRVIYDTYGSDEDMVVRLRQGNPGYDIVVPTNANVPTMIREGLLEK
                     VDHSKLANLGNLDATFMNLPFDPGNEYTIPYQWGTVGIGYNVDKVGEITSWADLFAYD
                     GPVAWLQDTTAMLGMGLLMVGKDPNSESAADIDEAAQFLIDHGSNVIYIAQDDGQEIL
                     ARGEADIVVEYSGDIFQIMDECECDTYAYVIPEEGTNFWVDSLAIPKGAPNKALAEAF
                     IDYVLWPQVAADISNYTAFGTPNLVALEEGLIDEDLAEDPAIYPSDETKARLFFAQQS
                     EAREPLLNDAWDMVKIFVGR"
BASE COUNT          266 a          507 c          431 g          216 t
ORIGIN      
        1 tgctgtccga cccgatcctc gacagggtcg tcaactcctc gggccgctga cggagcgcct
       61 ggcgcggcac gacgagccgt tgccgcaggt gctaaccttc cctctacgaa cgcgtcggca
      121 ggggtctcga agcactcgtg gccagcgcct gccgtccact tgagccgcgc tagcgcggag
      181 ctgcttgcaa gtcggaggac agatgcgtag aaccatcgct gcctgcctag tggccgtcgt
      241 cgtctcggcc gccgccgctc agacggactg gacctgcccg gacggcttcg cgggccagac
      301 cctgagcgtc tacaactggg ccacctacgt ggccgacgac accatctcca acttcgaggc
      361 tgcctgcggc gtgcgcgtca tctacgacac ctacggctcc gacgaggaca tggtggtgcg
      421 cctgcgccag gggaaccccg gctacgacat cgtcgtgccg accaacgcca acgtccccac
      481 catgatccgg gaagggctcc tggagaaggt cgaccacagc aagctcgcga acctcggcaa
      541 cctcgacgcc acgttcatga acctgccgtt cgacccgggc aacgagtaca cgatccccta
      601 ccagtggggc acggtcggca tcggctacaa cgtcgacaag gtgggcgaga tcacctcgtg
      661 ggccgacctg ttcgcctacg acggtccggt cgcgtggctg caggacacca cggccatgct
      721 cggcatgggc ctgctgatgg tcggcaagga ccccaacagc gagagcgccg ccgacatcga
      781 cgaggctgcc cagttcctca tcgaccacgg cagcaacgtc atctacatcg cccaggacga
      841 cggccaggag atcctggccc gcggcgaggc cgacatcgtg gtcgagtact ctggcgacat
      901 cttccagatc atggacgagt gcgagtgcga cacgtacgcc tacgtcatcc ccgaggaggg
      961 cacgaacttc tgggtagaca gcctcgccat ccccaagggc gcccccaaca aggccctcgc
     1021 cgaggccttc atcgactacg tcctctggcc gcaggtggcc gccgacatct cgaactacac
     1081 ggccttcggg acccccaacc tcgtggcgct cgaggagggc ctcatcgacg aggaccttgc
     1141 cgaggacccc gccatctacc cgagcgacga gacgaaggcg cgcctcttct tcgcccaaca
     1201 gtccgaggcg cgcgagccgc tcctcaacga cgcctgggac atggtcaaga tcttcgtagg
     1261 acgctgacct cagcgccgaa ccatgacgcc ggcgccagct gtacagatcc gcaacctggt
     1321 caaggagttc atcgaccctc gcaccaaggc ttccgttagg gcggtgaacg acatcgacct
     1381 ggagatccgc gagggcgagt tcttcgcgat gctcggcccg
//
LOCUS       DAOQYE010000687         1418 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_116590_length_1418_cov_1.796772, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000687 DAOQYE010000000
VERSION     DAOQYE010000687.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1418
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116590_length_1418_cov_1.796772"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="PKN52_10185"
     CDS             <1..411
                     /locus_tag="PKN52_10185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00361.1"
                     /translation="EGALFVLAYSGALIYVRSALRLPSPTLLSFGLLAPPTTGRSAAK
                     VPVVDAAQVAQTMMANRIVIVQDAGVPVGVTGLHHDRITSWEELVKVEADMAVTDLRR
                     LLAHEQLVVVLEGAEVRGIVTQEMYLAGLWGSVR"
     gene            complement(488..1414)
                     /gene="tsaD"
                     /locus_tag="PKN52_10190"
     CDS             complement(488..1414)
                     /gene="tsaD"
                     /locus_tag="PKN52_10190"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HNR00362.1"
                     /translation="MACGGEVLANAVESQTALHARFGGVMPELASREHLEVIDDVVSR
                     ALAQAGVGLADLDAVAATRGPGLVGALLVGLGYGKALAWGLNVPFVPVHHLEGHLAAA
                     GSEPPFVALLASGGHTVLFAVRDWDAIEELGRSRDDAAGEAFDKVARLLGLGYPGGAA
                     LARLAATGDDTAVALPFPMQGDKGLEFSFSGLKTAVALLLERDPGLTPADVAASFERV
                     VVGSLVSVTLRALERTGLPRLVVAGGVAANARLRSEFAAAGVDVSFPPPALATDNGAM
                     IALAGARRLSSGRVGERPLRTDAEPYLPLVGA"
BASE COUNT          203 a          518 c          520 g          177 t
ORIGIN      
        1 gagggcgccc tgttcgtgct ggcgtactcg ggagccttga tctacgtccg cagcgcgctg
       61 cgcctgccga gccccaccct cttgagcttc gggctgctcg ccccgcccac cacggggcgg
      121 tcggcggcga aggtgcccgt ggtcgacgcc gcgcaggtgg cgcagacgat gatggcgaac
      181 cggatcgtga tcgtgcagga cgccggcgtg cccgtcggcg tcacgggcct gcaccacgac
      241 cgcatcacct cgtgggagga gctcgtcaag gtcgaggccg acatggccgt caccgacctg
      301 aggcggctgc tggcgcacga gcagctcgtg gtggtgctcg agggcgccga ggtgagaggg
      361 atagtcacgc aggagatgta cctggcgggg ctctggggga gcgtgcgctg aagacgcggg
      421 aacgcggcac gcgacgcgac ttggcccgct cagcctgcca gccgccgtcg ctaccggtcg
      481 gtgctcgtca cgcgccgacc aagggtaggt agggctcggc atcggttcgc agcgggcgct
      541 ccccgacgcg gccggaactc agccgcctgg cgcccgccaa agcgatcatc gccccgttgt
      601 cggtcgccaa cgccggtggt gggaacgaca cgtcgacgcc cgccgcggcg aactcgctgc
      661 gcaacctcgc gttcgccgcc acccctccgg ccaccacgag gcgcggcagc cccgtccgtt
      721 ccagcgcgcg cagcgtcacg ctcacgagcg acccgaccac cacgcgctcg aacgaggcgg
      781 ccacgtcggc cggcgtcagg ccagggtccc gttccagcag gagtgccacg gcggtcttca
      841 ggccgctgaa ggagaactcc aggcccttgt cgccctgcat ggggaagggg agggcgacgg
      901 ccgtgtcgtc gccggtggcc gccaggcgcg cgagcgccgc tccgcccggg taaccgaggc
      961 ccaggaggcg cgccaccttg tcgaaggcct cgccggcggc gtcgtcccga ctgcggccga
     1021 gctcctcgat ggcgtcccag tcgcgcacgg cgaagaggac agtgtgaccg cccgaggcga
     1081 gcagggcgac gaagggcggc tccgagccgg cggccgcgag gtgcccctcg aggtggtgga
     1141 caggcacgaa cggtacgttc agaccccagg cgagcgcctt gccgtagccg agtccgacca
     1201 ggagggcgcc gaccagccca gggccgcgcg tggccgcgac ggcgtcgagg tccgcgagcc
     1261 ccactccagc ttgcgccagc gcgcggctca ccacgtcgtc gatgacctcg aggtgctcac
     1321 ggctcgcgag ctcgggcatc actccgccga agcgggcgtg cagggccgtc tgcgactcga
     1381 cggcgttcgc caggacctcg cctccgcacg ctatcccg
//
LOCUS       DAOQYE010000688         1417 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_116709_length_1417_cov_1.823789, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000688 DAOQYE010000000
VERSION     DAOQYE010000688.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116709_length_1417_cov_1.823789"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(126..1142)
                     /locus_tag="PKN52_10195"
     CDS             complement(126..1142)
                     /locus_tag="PKN52_10195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00363.1"
                     /translation="MYVEAFTQAKDPNAPAANEDRWLAHESRLFAVIDGVTDKSGEPL
                     PDGTTRGQAAGRLLDAQLRELAAGPLPAESSALVATLTDAIANAYKRAGLEGVVEASP
                     NLRFGAQLVAAFVTAEGWRLVAVGDCGARVTRCAGPPLLFGAATPHDDIAAIWRANVF
                     EHVLRRTGDVELALEAGRAYALAGNRQHLAAYRAVISERDQERLGLAAAHEAHAAHPD
                     LDRAAVDEVLRLGIARLGRYRNQSGPLGFAVLDGTAVHPAGVTDVRFERAEVESIELF
                     SDGYFGPPPGTAATVAAWERHIRHVEATDPHKVGAYRSTKGSSEGKFADDRTVMIVRQ
                     EAGA"
BASE COUNT          192 a          484 c          513 g          228 t
ORIGIN      
        1 gcagggagaa gaactccccc gcggcgacgt cgaggtgaac gtcgtccacg gccaccacgg
       61 ggccctcttt ggtctggaag accttggtga gcccggcgac tcctaacctc ccgtcgtgca
      121 cgcgttcacg ctccagcttc ctgccggacg atcatgacgg tgcgatcgtc ggcgaacttg
      181 ccctcgctcg atcccttggt gctacggtag gcgccgacct tgtgggggtc ggtggcctcg
      241 acgtgacgga tatggcgttc ccaggcggcg accgtggccg cagttcctgg tggtggtccg
      301 aagtagccgt cgctgaacag ttcgatgctc tctacctccg cgcgctcgaa gcgaacgtcg
      361 gtcacgcccg cgggatgcac ggccgtgccg tcgaggacgg cgaaccccaa cgggccagac
      421 tggttgcggt agcgacccaa gcgggcgatc ccaagcctga gcacctcgtc cacggccgcg
      481 cggtcgagat ccggatgcgc cgcgtgggct tcgtgggcgg ccgcaagtcc gaggcgttcc
      541 tggtcgcgct cggagatcac cgcccggtaa gccgcgaggt gctgtcggtt gcccgccaat
      601 gcgtaggctc tcccggcctc cagggccagc tcgacgtcgc cggtacgtcg caggacatgt
      661 tcgaagacgt tggctcgcca gatggcggcg atgtcgtcgt gcggagttgc cgcgccgaac
      721 aggagcggcg gtccggcgca cctggtcacg cgagcaccgc agtcgcccac cgccactagc
      781 cgccagcctt ccgccgtcac gaaggccgcc acgagttgcg ccccgaaacg gaggttcggg
      841 gaggcctcga cgacgccctc aagccccgcg cgcttgtagg cgttcgcgat cgcgtccgtc
      901 agggtcgcga cgagggcgga cgactccgcc gggagcggcc cggccgcgag ctcgcgaagc
      961 tgcgcgtcga gcagcctgcc cgccgcctgg ccccgggtcg tgccgtcggg gagcggttcg
     1021 ccactcttgt cggtgacgcc atcgatgacg gcgaacaggc gggactcgtg cgccagccaa
     1081 cggtcctcgt tggcggccgg cgcgtttggg tccttggctt gcgtgaacgc ttctacgtac
     1141 atggtcgaat cccggcccgg cggtcggtga agggtgcgac gctgcctcca aaacgggcgg
     1201 cccctcggcg tggtgccgcc gaggggccgc ccgcaggtcg gcaggtcagt tgcccgagaa
     1261 gacctcgtcg aacttcttga ggtccgcctc gcggttctgc gctgcgcgct ccgggtcgta
     1321 gtcgaaggtg ggcacttcgc tggtgggcgc gaggcccgct ggtgcagggg cgtccgcccg
     1381 gacggggcgg cggaagtgct tggcggcgac ctcgtag
//
LOCUS       DAOQYE010000689         1415 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_116891_length_1415_cov_3.302206, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000689 DAOQYE010000000
VERSION     DAOQYE010000689.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1415
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116891_length_1415_cov_3.302206"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..863)
                     /locus_tag="PKN52_10200"
     CDS             complement(<1..863)
                     /locus_tag="PKN52_10200"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HNR00364.1"
                     /translation="MFGSLVLSGTSTAFAQGKFKVVTTFTVIADMARNVAGDAAIVES
                     ITKPGAEIHDYQPTPGDVRRAQDADLILWNGLNLERWFERFFENLRGVPSAVVSDGVE
                     PIGIFEGPYEGLPNPHAWMSPTNALVYVDNIRDALAEHDPTNAAVYAANAEAYKERIE
                     SVIGPIKEELAAVPEEQRWLVTSEGAFSYLARDFGFKELYLWPINADAQGTPQQVRKV
                     VDAVRANGIPVVFSESTVSDKPAKEVARETGARYGGVLYVDSLTDANGPVPTYLDLLR
                     VTAETIAAAWW"
     gene            complement(1156..>1415)
                     /locus_tag="PKN52_10205"
     CDS             complement(1156..>1415)
                     /locus_tag="PKN52_10205"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HNR00365.1"
                     /translation="TTPLADLGEGGRFRVHRLMTRDRAVLAYLESRAVEPGVELVLRS
                     LEPGGNLLHLEFVGIGNGARGRKVALSRELASLVLGENLTN"
BASE COUNT          226 a          465 c          480 g          244 t
ORIGIN      
        1 tgccaccagg cggcggcgat cgtctcggcc gtgacgcgca ggaggtcgag gtacgtcggc
       61 actgggccgt tggcgtcggt cagcgagtcg acgtagagca cgccgccata acgcgcgccc
      121 gtctcccggg ccacttcctt ggcgggtttg tcggacaccg tgctctccga gaacaccacg
      181 gggataccgt tcgcgcgcac cgcgtcgacg actttcctca cctgctgcgg cgtcccttgc
      241 gcgtcggcgt tgatgggcca gaggtagagt tccttgaagc cgaagtcgcg cgccaggtat
      301 gagaaggcgc cctcgctcgt gaccaaccac cgttgctctt cgggcaccgc ggcgagttcc
      361 tccttgatcg gaccgatgac gctctcgatc cgttccttgt aggcctcggc gttggccgcg
      421 tacacggcgg cgttggtcgg gtcgtgctcg gccaacgcgt cccggatgtt gtccacatag
      481 acgagggcgt tggtaggcga catccaggcg tgcgggttgg gaagaccctc atacggcccc
      541 tcgaagatgc cgatcggctc gacgccgtcg ctcacgacgg cgctggggac gccccgcagg
      601 ttctcgaaga agcgctcgaa ccagcgttcc aggttgagac cgttccagag gatgaggtcg
      661 gcgtcctggg cgcgccgcac gtcacccggc gtcggttggt agtcgtggat ctcagcgcca
      721 ggcttagtga tggactcgac gatcgcggcg tcgccggcga cgttgcgcgc catgtcggct
      781 atgacggtga aagtggtgac gaccttgaac ttcccttgcg cgaacgccgt ggacgtgccg
      841 gacaagacca agcttccaaa catggccagg ctcatggcga gtgcgcggat cgggacgagg
      901 ggccaagcgc gtcgggacgg ggttcgcatc atgcctcctg aggtatctcg gcctcgacct
      961 gcggctaggt cgcggcctat tcgccgcggc tagtctaggg tttagcctcg actaatctag
     1021 cgtgttgcct cgatctcgtc aagcagccct cgacacgact cgaaggcggc tccgccccag
     1081 cggtcgcggg cgacccgact cggcttgtct gagatccgcg cgaccgtcag ggccgaggcg
     1141 gacaaccagc atcgatcagt tggtcaggtt ctcgcccaac acgagcgagg ccaactcgcg
     1201 ggagagcgcc accttgcgcc cgcgtgcgcc gttgccgatg ccgacgaact ctaggtggag
     1261 caagttgccg cccggctcga gcgacctgag aacgagctcg acgcccggct ctaccgcgcg
     1321 cgactctagg taggccagca cggcacgatc acgggtcatg aggcggtgga cgcggaaccg
     1381 cccaccctca cccaggtcgg ccagcggcgt ggtcg
//
LOCUS       DAOQYE010000690         1415 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_116915_length_1415_cov_2.688235, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000690 DAOQYE010000000
VERSION     DAOQYE010000690.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1415
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116915_length_1415_cov_2.688235"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(12..539)
                     /locus_tag="PKN52_10210"
     CDS             complement(12..539)
                     /locus_tag="PKN52_10210"
                     /inference="COORDINATES: protein motif:HMM:NF003191.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HNR00366.1"
                     /translation="MSEVASLALSAALIFSLRIVDVSLGTLRIGFLVRGQRRLAGLFG
                     FFESLVWLVAAAQVLSNLDSPVKFVAYAAGYATGTMLGVSIERWLAIGESIMRVVTPT
                     DTPPVEDQLRQAGYYVTVLNARGRDGDVRVSFSVIPRRKIPEMLRLLNSVNPAAFVTF
                     ENTTPVRTAAGGLRK"
     gene            complement(536..>1415)
                     /locus_tag="PKN52_10215"
     CDS             complement(536..>1415)
                     /locus_tag="PKN52_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNR00367.1"
                     /translation="DLGGTKILAAIVDEGRVVDSVRVDTPQTGFADVVAALAWSAREL
                     LDRGHAVVGVGIGSPGPLDKDRRKVLFAPNIAGMQDAPLVAELERSLGMRVVLENDAN
                     AAGYAEHLYGAAKDLESSVYVTVSTGIGGGLFIGDQVIRGANGLAGEIGHMTMMVGGP
                     MGGDGHTGSLEALAAGRSMARDASYAYGTEMGTRELFERARAGEPKALGIVDNAALFT
                     GIGLANLVKVFDPEGFVIGGGLAAVGEFYLSKIEAAAADYLRGYPMPLLRTALLGGEA
                     GVIGAAAVAAAESGRA"
BASE COUNT          226 a          516 c          469 g          204 t
ORIGIN      
        1 atgtgagagc gttacttgcg caagcctccg gcggccgtac gcaccggcgt ggtgttctcg
       61 aaggtcacga acgccgccgg gttgaccgag ttgagcagcc tgagcatctc cgggatcttg
      121 cggcgcggga tgaccgagaa agacacgcgc acgtccccgt cgcgacccct ggcgttcaac
      181 accgtcacgt agtaacccgc ctggcgcagc tggtcctcga cgggcggcgt gtcggtcggc
      241 gtgaccacgc gcatgatcga ctccccgatc gccagccacc gctcgatgct cacgcccagc
      301 atggtgcccg tcgcgtagcc ggccgcgtac gccacgaact tcacgggcga gtcaaggttc
      361 gagagtacct gcgcggccgc caccagccac accagcgact cgaagaagcc gaacagcccg
      421 gccaggcggc gctggccgcg cacgaggaag ccgatcctca aggtgccgag cgagacgtcg
      481 acgatgcgga gtgagaagat caacgccgcg gagagggcga gcgatgcgac ctcgctcacg
      541 cgcgccccga ctccgccgcc gccacggccg cggcgccgat gacgccggcc tcgcccccga
      601 gaagtgcagt gcgcaggagc ggcatcgggt agccgcgcag gtagtcggcc gccgcggcct
      661 ctatcttgct caggtagaac tcgcctaccg cggcgagacc gccgccgatg acgaagcctt
      721 ccgggtcgaa gaccttgacg aggttggcca acccgatccc cgtgaagagg gccgcgttgt
      781 cgacgatgcc cagggccttc ggctcgccgg cgcgcgccct ctcgaagagc tctcgggtgc
      841 ccatctcggt gccgtaggcg taagaggcgt ccctggccat ggagcgtccg gccgcgaggg
      901 cctcgaggct gcccgtgtgg ccgtcgccgc ccatcgggcc accgaccatc atggtcatgt
      961 ggccgatctc ccccgccagg ccgttggcgc ccctgatcac ctgatcgcct atgaacagcc
     1021 cgcctccgat gccggtcgag acggtgacgt acacgctcga ctccaggtcc ttggccgcgc
     1081 cgtacaggtg ctcggcatag ccggccgcgt tggcgtcgtt ctccagcacg acacgcatgc
     1141 caagggaccg ctccagctcg gccacgaggg gcgcgtcctg catgccggcg atgttcgggg
     1201 cgaagaggac cttgcgccgg tccttgtcga gcggccctgg cgaacctatg ccgacgccga
     1261 ccaccgcgtg cccgcggtcg agcagctcgc gcgcgctcca cgcgagggcc gccaccacgt
     1321 cggcgaaacc ggtttgcggc gtgtcgaccc tgacgctgtc gaccaccctg ccctcatcga
     1381 cgatggcggc gaggatcttc gttcctccca agtca
//
LOCUS       DAOQYE010000691         1414 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_117024_length_1414_cov_3.088300, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000691 DAOQYE010000000
VERSION     DAOQYE010000691.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1414
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117024_length_1414_cov_3.088300"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..819)
                     /locus_tag="PKN52_10220"
     CDS             complement(<1..819)
                     /locus_tag="PKN52_10220"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNR00368.1"
                     /translation="MEFRTFAAPAGERLDVAIAQALELSRTYAKELVSEGYVTVDGRP
                     VGKASAKLTGEEIVSVVLPPPRPMFVEPEDTDVHIIYQDDDLVAIDKPPGMVAHPTAT
                     LRTGTVVNALLGRMPLAKEWNQDPSDEAYRPGIVHRLDKDTSGVMVVAKNDEAHRHLS
                     HSFKKRFTQKEYVAIVVGEVADGLVVDAPIGRHSVRRQMMTVGGENQKTATTNFRVLG
                     RLPGHTLVKAIPRSGRTHQIRVHLAHVGAPILADEVYGHASSVLGRQALHAYRLT"
     gene            1003..>1414
                     /locus_tag="PKN52_10225"
     CDS             1003..>1414
                     /locus_tag="PKN52_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00369.1"
                     /translation="MGQPNGVQDTQPSASGPLPGPVALDAMGGERMPDAAVVGALAAQ
                     RLGHPVVLVGDEARLREELARHGGDLPIVHAPDVIGMDEHATDVRRRRESSVMVAMRL
                     VKEGRAAACVSMGHSGATMAAATYELGRLPGVARA"
BASE COUNT          196 a          476 c          516 g          226 t
ORIGIN      
        1 cgtcaggcgg taggcatgca gcgcctggcg cccgaggacg ctcgacgcgt gcccgtacac
       61 ttcgtcggcc aggatgggcg ctccgacgtg ggcgaggtgg acgcggatct gatgcgtgcg
      121 cccggagcgg gggatggcct tcaccaacgt gtgcccggga agacggccga ggacgcggaa
      181 gttcgtggtg gcggtcttct ggttctcgcc tccgaccgtc atcatctggc gccgcacaga
      241 gtggcgtccg atgggcgcgt cgacgacgag gccgtcggcg acctcgccga cgacgatggc
      301 gacgtactcc ttctgcgtga agcgcttctt gaacgagtgc gagaggtgac ggtgcgcctc
      361 gtcgttcttc gcgaccacca tcacgccgga cgtgtctttg tcgagccggt ggacgatgcc
      421 cggacggtag gcctcgtcgc tggggtcctg gttccactcc ttcgccaacg gcatccgccc
      481 gaggagggcg ttcacgaccg tgccggtgcg tagggtcgcc gtagggtgcg cgaccatgcc
      541 gggcggcttg tcgatggcga cgaggtcgtc gtcctggtag atgatgtgga cgtcggtgtc
      601 ctcaggctcc acgaacatgg ggcgcggcgg cggcaggacg acggagacga tctcctcgcc
      661 cgtgagcttg gcgctagcct tgcctaccgg ccgcccgtcg accgtgacgt acccctcgct
      721 cacgagctcc ttcgcgtacg tgcgcgagag ctcgagcgct tgggcgatcg cgacgtcgag
      781 tcgctcgccg gccggggccg cgaaggtacg gaactccact agagagagca tatccctagc
      841 cgtgacggct cgccgtccta accccgacgc cgggtgcgtg ctatcgctgg tgcgcgctgt
      901 cgtgggtagt ccgttgtcgc cggtcgcccc ctgtcgctgg tagcccgctg cctcgggtcg
      961 ctcgcgaacc gtacggagtc ggcggcgcta tactgaccgc gcatgggaca accgaacggc
     1021 gtccaggaca cgcaacccag cgcatcaggc ccactacccg gacccgtggc gctcgacgcc
     1081 atgggcggcg aacggatgcc ggacgccgcc gtcgtaggcg cgctagccgc gcagcgcctc
     1141 gggcatccgg tcgtgctcgt cggcgacgag gcgcgcttgc gcgaagagct ggcacggcac
     1201 gggggcgacc tgccgatcgt gcacgccccc gacgtcatcg gcatggacga gcacgccacc
     1261 gacgtcaggc gacgcaggga gtcgagcgtg atggtcgcca tgcggctcgt caaggagggg
     1321 cgtgcggcgg cgtgcgtctc catgggccac tccggggcca ccatggccgc cgccacgtac
     1381 gagctcgggc gcttgcctgg tgtggcccgc gccg
//
LOCUS       DAOQYE010000692         1414 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_117056_length_1414_cov_2.586461, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000692 DAOQYE010000000
VERSION     DAOQYE010000692.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1414
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117056_length_1414_cov_2.586461"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="PKN52_10230"
     CDS             <1..412
                     /locus_tag="PKN52_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HNR00370.1"
                     /translation="TGQNPLIGPNDDAYPRFPVMFDCYDPEYLRAARAAALAEGIHLR
                     EGVYLAISGPSYASRAELRAFRTLGADAIGMSTVFEVIRARHLGMRILGISTVTDMAL
                     PDRDEHATGDEVLAVAARTGATFRRLVRAVLPGL"
     gene            478..>1414
                     /locus_tag="PKN52_10235"
     CDS             478..>1414
                     /locus_tag="PKN52_10235"
                     /inference="COORDINATES: protein motif:HMM:NF016959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HNR00371.1"
                     /translation="MSRLTPLDIQHVEFSRRAGGYDRREVKAFLERLALEVEDALRES
                     QGLRKRLAAAEAEVARLRNAEAELQTAVMAAERMAGGMKESAKREAQHLLDDAERQRK
                     ARMADVEEGLLKSHARLEALEMQRKLFKEQFRALLQAYTTALEADDGVESDAALGTRG
                     TGAATRGDDTVLPRRRGGAARRHRQALTGSVAQARQRLVERLPGEHGALDPGGQLRYP
                     GEGLQVAQVQPRLLPANEALERGGQRSRLIRAPTADRARHEARRRLRDGAALPDEPRV
                     QLARPRSQVEGKLVAAVGVDTFLTHYAGPFQRAVTA"
BASE COUNT          213 a          516 c          505 g          180 t
ORIGIN      
        1 cacggggcag aacccgctca tcggaccgaa cgacgacgcc tacccgcggt tccccgtcat
       61 gttcgactgc tacgaccccg agtacttgcg ggcggcgcgc gccgccgcgc tggccgaggg
      121 catccacctc cgcgaaggcg tgtacctggc catcagcggg ccgtcgtacg cgagccgggc
      181 ggaactgcgc gcgttccgca ccctgggcgc agacgccatc ggcatgtcga cggtcttcga
      241 ggtcatacgc gcgcggcacc tcggcatgcg gatcctcggc atctccacgg tgaccgacat
      301 ggcgctgccg gaccgcgacg agcacgcgac gggcgacgag gtgctggccg tcgcggcacg
      361 caccggcgcc acgttccgcc gcctcgtgcg cgcggtcctg ccgggtctct agcgcatgga
      421 acgccaggtc actaccgcgg cgggtcgggg accgtccggc accggcgggg agggtgcatg
      481 agccgtctga cgccgctcga tatccagcac gtcgagttct cgcgccgcgc cggcggctac
      541 gaccggcgcg aggtgaaggc gttcctcgag cgcttggcgc tcgaggtgga ggacgcgctg
      601 cgcgagtccc aggggttacg caagcggttg gcggcggccg aggcggaggt cgcgaggctg
      661 cgcaacgcgg aggcggagct ccaaaccgcc gtcatggccg ccgagcgcat ggcgggaggc
      721 atgaaggagt cagccaagcg cgaggcgcaa cacctccttg acgacgccga gcgccagcgc
      781 aaggcgcgca tggcggacgt cgaggagggc ctcctcaagt ctcacgcgcg gctcgaggcg
      841 ctggagatgc agcgcaagct cttcaaagag cagttcaggg cgctgctgca agcctacacg
      901 acggctttgg aggcggacga cggcgtcgag tccgacgccg cgctcggaac ccgcgggacg
      961 ggagcggcga cgcgcggcga cgacaccgtc ctccctcgac gccgaggcgg cgctgctcga
     1021 cgacaccgtc aggccctaac cgggtccgtc gcccaggcgc gtcaacgcct cgtcgagcgt
     1081 cttccagggg agcacggcgc acttgacccg ggcgggcagc ttcgctatcc cggcgagggc
     1141 ctgcaggtcg cccaggtcca gccccggctc ctccccgcga acgaggcgct ggaacgcggc
     1201 ggacagcgca gccgcctcat ccgcgcccct accgctgatc gcgctcgtca tgaggctcgc
     1261 cgacgcctgc gagatggcgc agccctcccc gacgaaccgc gcgtccagct cgcccgcccc
     1321 cgctcgcagg tagagggtaa gctcgtcgcc gcagttgggg ttgacacctt cctgactcac
     1381 tacgccgggc ccttccagcg tgccgtgacg gcgc
//
LOCUS       DAOQYE010000693         1413 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_117151_length_1413_cov_3.659794, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000693 DAOQYE010000000
VERSION     DAOQYE010000693.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1413
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117151_length_1413_cov_3.659794"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="PKN52_10240"
     CDS             complement(<1..383)
                     /locus_tag="PKN52_10240"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HNR00372.1"
                     /translation="MTGLRLDRDIVAQRLELLDRAVDKLSTHRGLSRADLEADLELTW
                     MVQHGLLTCIQAVLDVSSHLVAALGAPTPSDYRGSIISLGRLGVVPPQLAERMAPMAG
                     FRNVLVHEYAAVDLEIVSSAINERLG"
     gene            complement(380..820)
                     /locus_tag="PKN52_10245"
     CDS             complement(380..820)
                     /locus_tag="PKN52_10245"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNR00373.1"
                     /translation="MSPISPAALERLRAYAAAHPEIAGVILFGSVARGEAHPESDLDI
                     GVLLTREANTRGMDWLHLVSDMAEIFGREDIDVAILNRATPLLMHRVVRDGHLLYAAN
                     NRAVAEFHLHAIQQYEDTRPLREMQYQRLLQQLDTPELKVEADR"
     gene            complement(990..>1413)
                     /locus_tag="PKN52_10250"
     CDS             complement(990..>1413)
                     /locus_tag="PKN52_10250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00374.1"
                     /translation="QYYWTLRADAPTTGATPRFAAWDAAVASTSFSAAYRSRYGQQAD
                     AVAWVAYTAVQVVQQAAESSRTDGIGLVRALARTDVDHALDDGMTNARMGADHQLHAP
                     IYTLRLTPPAAGARFQLADITELVGAFTPFGSEVEVCD"
BASE COUNT          234 a          459 c          460 g          260 t
ORIGIN      
        1 cctaggcgct cgttgatagc agagctcact atctctagat cgacagcggc gtattcgtgg
       61 acgagcacgt tacggaaccc ggccatgggg gccatgcgct cggcgagctg cgggggaacg
      121 acccctaggc gccccagaga gatgatgctg cccctgtaat cgctgggtgt tggcgcaccc
      181 aacgcggcca ccaggtggct ggagacgtcc agtacggcct ggatgcaggt cagcagcccg
      241 tgttgcacca tccaggtcaa ctctaggtcg gcctcgagat ctgcgcgcga cagcccccgg
      301 tgggtactca acttgtcgac ggccctgtca agaagctcga ggcgttgcgc cacgatgtcg
      361 cgatccagtc tcaaccccgt catctatccg cctctacttt cagctcgggg gtgtccagtt
      421 gctgaaggag gcgctgatac tgcatctcgc gcagcggccg tgtgtcctcg tactgttgga
      481 tggcgtgtaa gtggaactcg gctacggcgc ggttgttggc cgcgtaaagc agatggccgt
      541 cgcgcaccac ccggtgcatc aggagcggag tggcccggtt gaggatcgcc acgtcgatgt
      601 cctcgcgccc gaagatctct gccatgtccg agaccagatg cagccagtcc atcccccgcg
      661 tgttagcctc gcgcgttaga agcacgccga tatccaggtc gctctccggg tgcgcctcac
      721 ctcgcgcgac cgagccgaag aggatgacac ctgctatctc cgggtgcgca gcagcgtaag
      781 cgcgcagtcg ctccagggcg gcgggtgata taggactcat accaggggag tccggtggtc
      841 tgggaactgc gtccacgatt cagcctacac ggagtccacc tggttcgcgt cggtggccgt
      901 agtggcccga gccccaatgt cacgctcatc gccctcgacc gtacggaccg gtatctgcat
      961 gcctgtcgcg agcgtgccct ccggcgcctt cagtcgcaga cttccacttc cgaaccgaaa
     1021 ggggtgaagg cgccgacgag ttccgtgatg tcggccaact ggaacctcgc acccgccgcg
     1081 ggtggcgtga gcctcaacgt gtagatggga gcgtggagtt gatgatctgc ccccatacgg
     1141 gcgttcgtca taccgtcatc cagcgcgtga tcgacgtccg tgcgagccag ggctcgcacc
     1201 agcccgattc catccgttct gctcgactcg gcggcttgtt gcaccacctg cacggccgtg
     1261 tacgccaccc aggccacggc gtcggcctgc tggccgtagc gtgaacggta agccgcgctg
     1321 aagctggtgg aggcgaccgc cgcgtcccac gcggcgaacc tcggtgtggc gccggtggtc
     1381 ggggcgtcgg ctcggagcgt ccagtagtac tgt
//
LOCUS       DAOQYE010000694         1413 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_117172_length_1413_cov_2.896171, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000694 DAOQYE010000000
VERSION     DAOQYE010000694.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1413
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117172_length_1413_cov_2.896171"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="PKN52_10255"
     CDS             complement(<1..445)
                     /locus_tag="PKN52_10255"
                     /inference="COORDINATES: protein motif:HMM:NF015411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HNR00375.1"
                     /translation="MELVIVGLGRMGADMTRRLLQGGHAPVVTDLEGALVAELVEEGA
                     RGAATVAEAALQLSSPRVVWSMVPAGDPTEHVFGQALAVLSAGDVFVDGANSNWEDSS
                     RRAAKADAAGVLWLDAGVSGGVWGLENGYNLMVGGSETAFELTRPV"
     gene            502..>1413
                     /gene="tyrS"
                     /locus_tag="PKN52_10260"
     CDS             502..>1413
                     /gene="tyrS"
                     /locus_tag="PKN52_10260"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HNR00376.1"
                     /translation="MRDKLPAVNGTSDVEAPDAGEHAEALALLNRGAEHIVPDGGLLT
                     KLRLAAKEGRQLRAKLGVDPSSSDLHVGHAVVLRKLRQFQDLGHRVVLIIGDFTAMIG
                     DPSGRSKTRPVLTLEETRRNGETYVAQAVKVLDTDPDKLEIRHNSEWLEPLGFADVIR
                     LASNYTVARMLERDDFTKRFQSGVPISVHEFLYPLAQAYDSVAIRADVELGGTDQLFN
                     LLVGRDVQRAYGQEPQVALTTPLLVGLDGVEKMSKSLGNYIGIAEPADVMFKKAMQVA
                     DALLVQYAELCTALDLEAVRARLAADPV"
BASE COUNT          229 a          513 c          468 g          203 t
ORIGIN      
        1 ggacgggccg cgtcagctcg aacgccgtct cggagccgcc caccatcagg ttgtagccgt
       61 tctcgagccc ccagacgccg ccggagacgc cggcgtcgag ccagagcacc cctgccgcgt
      121 ccgccttggc cgccctgcgg ctcgagtcct cccagttcga gttcgcgccg tcgacgaaca
      181 cgtcgcccgc agacagcacg gccaacgcct gaccgaagac atgctcggtc gggtcgccgg
      241 cagggaccat cgaccacacg acgcggggcg acgacagctg gagggcggcc tcggcgaccg
      301 tggcggcgcc ccgcgcgccc tcctcgacga gttcggcgac gagggcccct tcgaggtccg
      361 tcaccaccgg agcgtgcccg ccctgcaaca gccggcgggt catgtcggcg cccatgcgcc
      421 cgagtccgac tatgacgagt tccattctct aagcctcccg ggcgcgtcca tcggcgccgt
      481 caccgtcaga tgctaccagg ggtgcgtgat aagctgcccg ccgtgaacgg gacatcggat
      541 gtggaggcgc ctgacgccgg tgagcacgcc gaggcgctcg ccctgctcaa ccggggggcc
      601 gagcatatcg tgccggacgg cggcctgctg acgaagctgc gactggcggc caaggagggc
      661 agacagctca gggcgaagct tggggtcgac ccgtcgagct cagacctgca cgtcggccac
      721 gcggtcgtgc tgcgcaagct gaggcagttc caggacctcg gtcaccgcgt ggtgctaatc
      781 atcggcgact tcacggccat gatcggcgac ccgtccggcc gcagcaagac gcgaccggtg
      841 ctcacgttgg aggagacgcg gcgcaacggc gagacctacg tcgcccaagc cgtcaaggtc
      901 ctcgacaccg accctgacaa gctcgagatc cgccacaact ccgagtggct ggagccgctc
      961 ggcttcgccg acgtcatccg gttggcctcg aactacaccg tggcgcgcat gctcgagcgc
     1021 gacgacttca cgaagcgctt ccagtcgggc gtgccgatct ccgtgcacga gttcctctac
     1081 ccgttggcgc aggcctacga ctcggtggcc attcgcgccg acgtcgagct gggcggcacg
     1141 gaccagctct tcaaccttct cgtcggacgc gacgtgcagc gggcgtacgg tcaggaacct
     1201 caggtcgcgc tcacgacccc gctcctcgtc ggtctcgacg gggtcgagaa gatgtccaag
     1261 tcgctcggca actacatcgg catcgccgag ccggccgacg tcatgttcaa gaaggccatg
     1321 caggtggccg acgcgctcct cgtccagtac gcggaactct gcacggcgct cgacctcgaa
     1381 gcggtgcggg cgcggctcgc ggccgacccc gtc
//
LOCUS       DAOQYE010000695         1412 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_117288_length_1412_cov_3.374355, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000695 DAOQYE010000000
VERSION     DAOQYE010000695.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1412
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117288_length_1412_cov_3.374355"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /locus_tag="PKN52_10265"
     CDS             complement(<1..514)
                     /locus_tag="PKN52_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNR00377.1"
                     /translation="MSGIKLVGPVPGPKSLAMAARRDAAGARGASRLTDLAVVGGHGP
                     YVIDADGNRLLDFAGGIGVLAVGHTPEGVVSAMQDQAERLIHLCAIVGTYEPYVRVLE
                     TLCELAPGDFPKKAVLMNSGAEALETAVNVARAYTGRQAVVVFEGAYHGRTNLTLGMT
                     SKYALFKKGFG"
     gene            complement(511..>1412)
                     /locus_tag="PKN52_10270"
     CDS             complement(511..>1412)
                     /locus_tag="PKN52_10270"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNR00378.1"
                     /translation="AMALTTSKTVYRHRYQPLPAGVVVAPFPYAYRYGWDDDTTVDFC
                     LRELELVLKSQTAPDETAAFVIEPVLGEGGYVPAPKRFLEGLRRLADEHGIVLVFDEV
                     QTGFGRTGKFFAYEHHGVVPDVVILAKGLGSGLPISGIAARPELTAKWEKGTHGGTYG
                     GGSTVPLAAARATIDAMIAEDLPGNAARMGERLVDGLRELQRRHPVIGDVRGLGLMIG
                     VEFAGEAGVAKRVQQACLDERLLLLTCGVNDQVVRWIPPLVVGENEIDAALATFGRAL
                     AAAGAEAAPLAVAGSAPRAGERG"
BASE COUNT          190 a          493 c          528 g          201 t
ORIGIN      
        1 gcccgaagcc cttcttgaag agcgcgtact tgctcgtcat gccgagcgtg aggttcgtgc
       61 gcccgtggta ggcgccctcg aacacgacga cggcctgccg gcccgtgtag gcgcgcgcga
      121 cgttgacggc cgtctcgagc gcctcggcgc cgctgttcat gagcacggcc ttcttgggga
      181 agtcgccggg ggccaactcg cagagggtct cgaggacgcg cacgtacggc tcgtacgtgc
      241 cgacgatggc gcagaggtgg atgaggcgct cagcctggtc ctgcatggcg ctgaccacgc
      301 cctcgggcgt gtgaccgacg gccaggacac cgatgccgcc cgcgaagtcg aggagccggt
      361 tgccgtccgc gtcgatcacg tacgggccgt gaccgccgac cacggcgagg tcggtcaggc
      421 gcgaggcgcc gcgggcgcct gccgcgtcgc ggcgggccgc catggctagg ctcttgggtc
      481 cggggaccgg gccgacgagc ttgatgccgc tcatccgcgc tccccggcgc gcggggccga
      541 gccggcgacg gccagcgggg cggcctctgc tccggcggcc gcgagggcgc ggccgaaggt
      601 ggccagcgcg gcgtcgatct cgttctcgcc gacgacgagc ggtgggatcc agcgcacgac
      661 ctggtcgttg accccgcacg tgaggagcag gaggcgctcg tccaggcaag cctgctggac
      721 gcgcttcgcg acgccggcct cgccggcgaa ctccacgccg atcatcaggc caagcccgcg
      781 cacgtcgccg atgacggggt gtcggcgctg gagctcgcgc aacccgtcaa cgagcctctc
      841 gcccatccgg gctgcgttgc cgggcaggtc ctcggcgatc atcgcgtcga tcgtggcgcg
      901 cgcggcggcc aacggcacgg tgctgccgcc cccgtacgtg ccgccgtggg tgcccttctc
      961 ccacttggcc gtcagttcgg ggcgagcggc aatgcccgag atggggaggc cgctgccgag
     1021 ccccttggcg aggatcacca cgtccgggac gacgccgtgg tgctcgtagg cgaagaactt
     1081 gcccgtgcgc ccgaagcccg tctgcacctc gtcgaacacg agcacgatgc cgtgctcgtc
     1141 ggcgagacgc ctcagccctt cgaggaagcg cttgggggcc ggcacgtagc cgccctcgcc
     1201 gagcacgggc tctatgacga acgcggccgt ctcgtccggc gccgtctggc tcttgaggac
     1261 gagctcgagc tcgcgcaggc agaagtcgac ggtcgtgtcg tcgtcccagc cgtagcggta
     1321 ggcgtacggg aaaggcgcga cgacgacccc ggccgggagc ggctggtagc ggtggcggta
     1381 gacggtcttc gaggtcgtga gggccatcgc ct
//
LOCUS       DAOQYE010000696         1412 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_117382_length_1412_cov_1.879882, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000696 DAOQYE010000000
VERSION     DAOQYE010000696.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1412
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117382_length_1412_cov_1.879882"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="PKN52_10275"
     CDS             complement(<1..181)
                     /locus_tag="PKN52_10275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00379.1"
                     /translation="MNHFPGRRAAILLGAHLLALGLAGCASLGLHEPPTVNLVGIEPI
                     AGEGLESRFELRLRIQ"
     gene            268..1074
                     /locus_tag="PKN52_10280"
     CDS             268..1074
                     /locus_tag="PKN52_10280"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HNR00380.1"
                     /translation="MAMIRAHRLGGRALLALGLAATIAAPAAAQPKLPDTLSWSAYDV
                     GSGGYNQAVAIGNALKQKYGVNLRVLPGKNDVSRTLPLREGKVQFSANGVGGTYLSQE
                     GVYEFGAKNWGPQPVRALMLNTSDQLLTVLAAGDVGIKTVADLKGKRVAWVIGAPSLN
                     QNITAILAHANLTWDDVKKVEFGGFGAAMDGIVNNQVDAAFSSSISGKAYAIAKSPRG
                     LVYPIISHADKEGWKRMNAIAPFFFPFMGTEGADLSKEKPAEAATYPSRS"
     gene            1074..>1412
                     /locus_tag="PKN52_10285"
     CDS             1074..>1412
                     /locus_tag="PKN52_10285"
                     /inference="COORDINATES: protein motif:HMM:NF028178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HNR00381.1"
                     /translation="MTYADRDVDTVYNMTKAMVEAFGDYKDNAPGNSGWDAKRQNFAW
                     VVPYHDGAIKYWKEAGLWKPEHQAHNDKLIERQKVMATAWAEVSKGSHADDEAFKKAW
                     AKARADALTKA"
BASE COUNT          267 a          488 c          466 g          191 t
ORIGIN      
        1 tctggatccg cagtcgcagc tcgaagcgcg actccaggcc ttcgccggcg atcggctcga
       61 tcccgaccag gttgaccgtc ggcggctcgt gcaggccgag cgaggcgcag ccggccaggc
      121 cgagcgcaag gagatgggcg ccgagcagga tcgccgcgcg ccgtccaggg aagtggttca
      181 tcgtggtgcc tccgtaggga tcaggataac tggatcgcgc cggcggggct ctgctaggat
      241 cggtcctcca acaacgagga tggagacatg gccatgatcc gagcccaccg actcggaggc
      301 cgcgcgctgc tcgcgctcgg cctcgccgca acgattgccg cacccgccgc cgcccagccc
      361 aagctgcccg acacgctgtc ctggagcgcc tacgacgtgg gctccggcgg ctacaaccag
      421 gccgtcgcca tcggcaacgc cctcaagcag aagtacggcg tgaacctgcg cgtgctgccc
      481 ggcaagaacg acgtctcgcg cacgctgccg ctgcgcgagg gcaaggtcca gttctcggcc
      541 aacggcgtgg gcggcaccta cctgtcccag gaaggcgtct acgagttcgg cgcgaagaac
      601 tgggggccgc agccggtccg cgcgctgatg ctcaacacct ccgaccagct gctcaccgtg
      661 ctggccgccg gcgacgtggg catcaagacc gtcgccgacc tgaagggcaa gcgggtggcc
      721 tgggtgatcg gcgcgccgtc gctgaaccag aacatcaccg ccatcctggc gcatgccaac
      781 ctcacctggg acgacgtcaa gaaggtcgag ttcggcggct tcggcgccgc catggacggc
      841 atcgtcaaca accaggtcga cgcggcgttc agctcgtcga tctcgggcaa ggcctacgcg
      901 atcgccaagt cgccacgtgg cctggtgtac ccgatcatct cgcatgccga caaggaaggc
      961 tggaagcgga tgaacgcgat cgcgccgttc ttcttcccct tcatgggcac cgagggggcc
     1021 gacctgtcca aggagaagcc ggccgaggcc gccacctacc cctcccggtc ctgatgacct
     1081 acgccgatcg cgacgtcgac acggtctaca acatgaccaa ggcgatggtc gaggccttcg
     1141 gcgactacaa ggacaacgcc ccgggcaaca gcggctggga tgccaagcgc cagaacttcg
     1201 cgtgggtcgt gccctaccac gacggggcga tcaagtactg gaaggaagcc ggcctctgga
     1261 aacccgagca ccaggcgcac aacgacaagc tcatcgagcg ccagaaggtc atggccactg
     1321 cctgggctga agtctccaag ggtagccacg cggacgacga ggcctttaag aaggcctggg
     1381 ccaaggcgcg cgccgacgcg ctcaccaagg cg
//
LOCUS       DAOQYE010000697         1409 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_117723_length_1409_cov_3.022895, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000697 DAOQYE010000000
VERSION     DAOQYE010000697.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117723_length_1409_cov_3.022895"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="PKN52_10290"
     CDS             <1..448
                     /locus_tag="PKN52_10290"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178463.1"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HNR00382.1"
                     /translation="NASTLNDGAAALVITSEDVAASRGMPVLARIHGWSGAAVAPEWF
                     TVAPVEATRKLFDKLGWTAESVDIFEVNEAFSVVPLAVMEEFGLGAESVNVLGGAVAL
                     GHPIGASGARILVTLMNALRLKGKRRGVASICIGGGEGLALGLELA"
     gene            complement(481..1224)
                     /locus_tag="PKN52_10295"
     CDS             complement(481..1224)
                     /locus_tag="PKN52_10295"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNR00383.1"
                     /translation="MIIGVISDVHANVIALEAALEAMRRRGVDTVVCLGDLVGYGPAP
                     NETLDLIRSEHVMCTMGAADERLAYGFAATAKRRQGVADDILAWTLGVLEPTHLSFLR
                     SLPIQKRLSTPLGWMRFFHGTLDNAGERLNLNQDPHSLTRLLERNRCTILAAGATHVP
                     FFRRVGPTGLVVNPGSVGLSLNGEPGADYAVLTIDEGSVEIEMDKVEYDYAAVAFEIV
                     AWGISPMVAEAVQMGRLPEKLAHQDEGRG"
BASE COUNT          195 a          475 c          516 g          223 t
ORIGIN      
        1 caacgccagc accttgaacg acggggcggc ggctctggtc atcacgagcg aggacgtggc
       61 cgcgagccgc ggcatgccgg tgctcgcccg catccacggt tggtcgggcg ccgcggtggc
      121 gccggagtgg ttcaccgtcg ccccggtcga ggctacgcgc aagctgttcg acaagctggg
      181 ctggacagcc gagagcgtcg acatcttcga ggtcaacgag gctttcagcg tcgtgccgct
      241 ggccgtgatg gaggagttcg gcttgggcgc cgaaagcgtg aacgtgctcg gcggcgccgt
      301 cgccctcggt caccccatcg gggcgtcggg cgcgcggatc ctcgtgacct tgatgaacgc
      361 gctgcggctc aagggcaagc gccgcggcgt cgccagcatc tgcatcggcg gtggcgaggg
      421 gttggcgttg gggttggaac tggcctgagc gacgcttttg ccgcgggtct ctgggcggcc
      481 tcagcccctg ccctcgtcct ggtgagccag cttctccggc agccgcccca tctgcacggc
      541 ctcggccacc atcggcgaga tgccccacgc cacgatctcg aaagccacgg cggcgtagtc
      601 gtactccacc ttgtccatct ctatctcgac cgagccttcg tcgatggtca ggaccgcgta
      661 gtcggcgccc ggttcgccgt tgagtgacag accgacggag ccggggttga ctactaggcc
      721 cgtcggcccc acgcggcgga agaacggcac gtgggtggcg ccggccgcca ggatggtgca
      781 gcggttgcgt tccaggaggc gcgtcagcga gtgggggtcc tggttcaggt tgagccgttc
      841 cccagcgttg tcgagcgtgc cgtggaagaa gcgcatccac cccaggggcg tggagaggcg
      901 tttctggatg ggcaggctcc tcaggaacga caggtgcgtc ggctccagga cgccgagcgt
      961 ccaggcgagg atgtcgtccg ccacgccctg ccggcgcttg gcggtggccg cgaagccgta
     1021 ggcgagccgc tcgtcggcgg cgcccatggt gcacatgacg tgctcggagc ggatgaggtc
     1081 gagcgtctcg ttaggcgccg gcccgtagcc gaccaggtcc ccgaggcaca ccacggtgtc
     1141 gaccccgcgc cgcctcatgg cctccagggc ggcctcgagg gcgatgacgt tcgcgtggac
     1201 gtcgcttatg accccgatga tcacggcagc accccgccgc ggctcgcgcg tcggcgcgcc
     1261 ccgccaccgg ccgcgcggcc ggtggccatc agcggctggc ttgcgtcagt cgcggccacc
     1321 gagttggacg ccttcgcgca ggaaggccgt caacagctgg acggcgccct cgaggtccga
     1381 cttcgagacc atctcggtga cggtgtgga
//
LOCUS       DAOQYE010000698         1409 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_117743_length_1409_cov_2.717873, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000698 DAOQYE010000000
VERSION     DAOQYE010000698.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117743_length_1409_cov_2.717873"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..922)
                     /locus_tag="PKN52_10300"
     CDS             complement(<1..922)
                     /locus_tag="PKN52_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNR00384.1"
                     /translation="MALENAASQPATDAARAPTAGPAVPRSLAAATSYLSKHDAERLA
                     EAYAFAEEHHRGMLRRSGEPFINHPVAVTEILAEMHLDISALMAGLLHDTVEDTSATF
                     EEIEEKFGETVRRIVEGETKISKLAVRVYENEQAENLRQMLLAMTGDVRIILVKLADR
                     LHNMRTLRFMPPHKQLSISEETIEIFAPLAHRLGINHIKNELEDIAFRYLEPERYLQL
                     QRQVRMRQSEREAYVKKSIKLLEERLRQEGLKFELSGRSKHLYSIHRKMLRDHRNLDQ
                     IFDLMAIRAVLDPGENDLGGGSGGGPAMPNE"
     gene            1174..>1409
                     /locus_tag="PKN52_10305"
     CDS             1174..>1409
                     /locus_tag="PKN52_10305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00385.1"
                     /translation="MRELRPPPVQAQTLAHELLLSRVEACVWTTDKDLRVTSWAGFDL
                     LGSDQGEDALFGSSVAELFAGTGRPDEYVAAHRQA"
BASE COUNT          193 a          451 c          496 g          269 t
ORIGIN      
        1 cctcgttcgg catggcgggg ccgccgccgc taccgccgcc gaggtcgttc tcacccgggt
       61 cgagcacggc gcggatggcc atgaggtcga agatctggtc gaggttgcgg tggtcgcgca
      121 gcatcttgcg gtggatgctg tacaggtgct tgctgcggcc ggagagctcg aacttgaggc
      181 cctcctggcg caggcgctcc tccagcagct tgatgctctt cttgacgtac gcctcgcgct
      241 cggactggcg catgcggacc tggcgctgca gctgcaggta acgctcgggt tcgaggtagc
      301 ggaacgcgat gtcctcgagc tcgttcttga tgtggttgat accgagccgg tgcgcgagcg
      361 gcgcgaagat ctcgatggtc tcctcgctga tggagagctg cttgtgcggc ggcatgaacc
      421 gcagggtgcg catgttgtgc agccggtcgg cgagcttgac gaggatgatg cgcacgtcgc
      481 cggtcatggc gaggagcatc tgccgcaggt tctccgcctg ctcgttctcg tagacgcgca
      541 cggcgagctt gcttatcttc gtctcgccct cgacgatgcg cctgaccgtc tcgccgaact
      601 tctcctcgat ctcctcgaag gtggcgctcg tgtcctccac cgtgtcgtgc aggagcccgg
      661 ccatcagggc gctgatgtcg aggtgcatct cggcgaggat ctcggtgacg gcgacggggt
      721 ggttgatgaa cggctcgccc gagcgccgca gcatgccgcg gtggtgctcc tcggcgaacg
      781 cgtaggcctc ggcgagcctc tcggcgtcat gcttggagag gtagctggtg gcggcggcga
      841 ggctcctcgg cacggcgggg ccggccgtcg gggctcgggc ggcgtccgtg gctggctggc
      901 tagcggcgtt ctcgagggcc atcaggccat tgaggttacc actagcagcg gtcgcggctc
      961 gacttctggc gcgtactcac cgtgctgtag cgggcccgag ctcccggtcg gcgtccggtc
     1021 gacgaacccg ccgcgatcgc gctcctcgat cccccgccgg cctgattaat taccccgtaa
     1081 ctcggcctgg cttaacatga ccgtcaccgc tttcgtgatg accatgccac tgtcggggcg
     1141 gcggggccgg tcgggaaggt caggggcaac gacatgcggg aacttcgccc accgcccgtg
     1201 caggcgcaga ccctagctca cgagctgctc ttgagccgcg tcgaggcctg cgtctggact
     1261 accgacaaag acctgcgcgt tacctcatgg gcagggttcg acttgctcgg ttccgaccaa
     1321 ggtgaagacg cactcttcgg ctcgagcgtg gccgagttgt tcgccggcac cggcaggccc
     1381 gacgagtacg tggcagctca ccgtcaggc
//
LOCUS       DAOQYE010000699         1409 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_117770_length_1409_cov_2.283604, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000699 DAOQYE010000000
VERSION     DAOQYE010000699.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117770_length_1409_cov_2.283604"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1409
                     /locus_tag="PKN52_10310"
     CDS             <1..>1409
                     /locus_tag="PKN52_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNR00386.1"
                     /translation="RAVDLGAQAPKKLAEIGVTTYRELLWHLPRRYEDRRVLPDYGSL
                     QDGATATVSGQVVSRIGNRTKRGLHVTRAALTDGRGARITAVWFNQPWLEKQLYPGLR
                     LVLSGKVKRQGRLLELGVQGFEVDDDSESLSFGRIVGIYPTTQGLSQAYLRRAAKRLL
                     EAMPTVQDYLQRSILDDHALVPLDQAMRSAHFPDSEEGLQAALRRLKFDDFLFFQLAV
                     LRSRDRAAVSRAFEVRQEYLDDFFSTLPFELTEYQGRALREILADLAQPRQMARLLQG
                     DVGSGKTAVAAAAVYVAVRNGAQAALMAPTEILARQHFTNLQHYLYPLGVRMELLTGA
                     MTAGERRAARERIGTGQSDFAVGTHALIQDGVTFRDLGLAVIDEEHRFGVEQRRTLIK
                     TMPDVLVMSATPIPRSLALTDYGDLELTTIEGLPPGRVPIKTALTSATKRRDVYRFAW
                     EQIREGRQVYVVTPLIEES"
BASE COUNT          223 a          512 c          471 g          203 t
ORIGIN      
        1 cgcgcggtcg acctgggcgc ccaggcgccg aagaagctcg ccgagatcgg tgtgacgacc
       61 taccgcgagc tcctctggca cctcccgagg cgttacgagg accggcgcgt gctgcccgac
      121 tacggcagcc tccaggacgg cgccaccgcc accgtctccg gtcaggtcgt cagccggata
      181 ggtaaccgca ccaagcgggg tctgcacgtc acgcgcgccg ccctgaccga cggccggggc
      241 gcgcggatca cggccgtctg gttcaaccag ccgtggctcg agaagcagct gtacccgggc
      301 ctcaggctcg tcctctccgg caaggtgaag cgccagggac ggctcctgga gctcggcgtg
      361 caaggcttcg aggtcgacga cgacagcgag tcgctttcct tcgggcgcat cgtcggcatc
      421 tacccgacga cgcaggggct gagccaggcg tacctgcggc gcgccgccaa gcgcctcctc
      481 gaggccatgc cgacggtaca ggactacttg caacgttcca tcctcgatga ccacgcgctc
      541 gtccccctcg atcaggccat gcgctcggcc cacttcccag acagcgagga gggtttgcag
      601 gccgccctca ggcgcctgaa gttcgacgac ttcctcttct tccagctcgc cgtcctgcgc
      661 agccgcgacc gcgcggccgt gtcgcgggcc ttcgaggtaa ggcaggagta cctggacgac
      721 ttcttctcga cgctgccctt cgagctgacg gagtaccagg gtcgggcgct gcgcgagatc
      781 ctcgccgacc tcgcccagcc gcggcagatg gcgcgcctgc tgcagggcga cgtcgggtcg
      841 gggaagacgg ccgtggccgc cgccgccgtg tacgttgccg tgcgcaacgg cgcgcaggcc
      901 gccctgatgg cacccacgga gatcctcgcc aggcagcact tcaccaacct ccagcactac
      961 ctgtacccgc tcggcgtgcg tatggaactg ctcaccggcg cgatgacggc gggtgagcgc
     1021 cgcgccgcca gggagcgcat cggcacgggc cagtcggact tcgccgtcgg cacgcacgcg
     1081 ctcatccagg acggcgtgac gttcagggac cttgggttgg cggtcatcga cgaggagcat
     1141 cgcttcgggg tcgagcagcg gcgcaccctc atcaagacca tgccggacgt gctggtgatg
     1201 agcgccacac cgatcccccg ctcgctcgcc ctgacggact acggcgacct cgagctcacc
     1261 accatcgagg gactgccgcc tgggcgggtg cccatcaaga cggccctgac gagcgccacc
     1321 aagcggcgcg acgtctaccg cttcgcctgg gagcagatac gcgaggggcg ccaggtctac
     1381 gtcgtcacgc cgctgatcga ggagagcga
//
LOCUS       DAOQYE010000700         1407 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_117980_length_1407_cov_3.507396, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000700 DAOQYE010000000
VERSION     DAOQYE010000700.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1407
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117980_length_1407_cov_3.507396"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..906
                     /locus_tag="PKN52_10315"
     CDS             <1..906
                     /locus_tag="PKN52_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNR00387.1"
                     /translation="NLTLGMTSKYALFKKGFGPFAPEIYRLPFPDLYRRPAGMAEDAF
                     LDMAIAALDKAMVAQVDPAAIAAFVIEPVQGEAGFLPVPARFLRRLRELADRYGIVLV
                     ADEVQSGLARTGKLWAVEHSGVVPDLVTTAKSLAAGMPLSAVVGRAEMMDAPHPGGLG
                     GTYSGNPLACAAALEALAMLSSEAFLTRAVQVGERLRGGLERIAAEQATVGEVRGLGP
                     MLAMEFVKDRVTKEPFPELVLDVTKQALKRGLIVIRAGLYSNCIRLLPPLNMTDAEVD
                     EGLAVLREAVAAAVALHAPVAEPVA"
     gene            911..>1407
                     /locus_tag="PKN52_10320"
     CDS             911..>1407
                     /locus_tag="PKN52_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNR00388.1"
                     /translation="MTTDRFPLKQLIGGEWVAAASGGTWDLIDPGSERVVQRVPFGGA
                     ADARAGVDAAAAAYPAWAAKTAYERAEVLERAAAWIRANVDDLAPITTEESGKPLGDA
                     RGEWLSATGYLTWFAGEGIRAYGRTVPARVAGRRISVLPQPLGVVATITAWNFPVYNI
                     VRTWAA"
BASE COUNT          185 a          526 c          501 g          195 t
ORIGIN      
        1 aacctcacgc tcggcatgac gagcaagtac gcgctcttca agaagggctt cgggccgttc
       61 gcgcccgaga tctaccgcct gccgttcccc gatctctacc gtcggcccgc cggcatggcg
      121 gaggacgcct tcctcgacat ggccatcgcc gcgctcgaca aggccatggt cgcgcaggtc
      181 gacccggccg ccatcgccgc cttcgtcatc gagccggtgc agggcgaggc gggcttcctg
      241 cccgtaccgg cgcgcttcct gcgccggctg agggaactcg ccgaccggta cggcatcgtg
      301 ctcgtcgccg acgaggtgca gtcgggcctg gctcgcactg gcaagctgtg ggcggtcgag
      361 cactctggcg tcgtgcccga cctcgtcacg acggccaagt cgctcgccgc cggcatgccg
      421 ctctcagcgg tcgtcggccg cgccgagatg atggacgcgc cgcaccccgg cggccttggc
      481 ggcacctact cgggcaaccc gctcgcctgc gcggcggcgc tcgaggccct cgccatgctc
      541 agcagcgagg ccttcctgac gcgcgccgtg caggtgggtg agcggctgcg cggcggcctc
      601 gagcggatcg ccgccgagca ggcgacggtc ggcgaggtgc gcggcctcgg cccgatgctc
      661 gccatggagt tcgtgaagga ccgcgtcacg aaggagccgt tccccgagct cgtgctcgac
      721 gtcacgaagc aggcgctgaa acgcggcctc atcgtcatcc gggccggcct ctactcgaac
      781 tgcatccgcc tcctgccgcc actcaacatg acggacgcgg aagtcgacga gggcctcgcc
      841 gtcctgcgcg aggcggtggc cgctgccgtg gcgctgcacg cgccggtggc cgagccggtc
      901 gcctaacgcg atgacgaccg accgtttccc gctcaagcag ctcatcgggg gtgagtgggt
      961 cgccgccgcc tccggcggga cgtgggacct gatcgacccc ggcagcgaac gcgtcgtgca
     1021 gcgcgtgccg ttcggtggtg ccgccgacgc gagggccggg gtggacgccg ccgccgccgc
     1081 gtacccggcc tgggccgcga agaccgcgta cgagcgcgcc gaggtcctgg agcgcgccgc
     1141 cgcctggatc agggcgaacg tcgacgacct cgcgcccatc acgaccgagg agtccggcaa
     1201 gcccctgggc gacgcgcgcg gcgagtggct ctccgccacg ggctacctga cgtggttcgc
     1261 gggcgagggc atccgcgcct acgggcgcac cgtgcccgcc agggtggcgg gccggcgcat
     1321 cagcgtcctg ccgcagcccc ttggggtcgt cgccaccatc acggcctgga acttccccgt
     1381 ctacaacatc gtgaggacct gggcggc
//
LOCUS       DAOQYE010000701         1407 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_118003_length_1407_cov_2.789941, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000701 DAOQYE010000000
VERSION     DAOQYE010000701.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1407
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118003_length_1407_cov_2.789941"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1357)
                     /locus_tag="PKN52_10325"
     CDS             complement(<1..1357)
                     /locus_tag="PKN52_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNR00389.1"
                     /translation="MALLWRTAPAKVTLLTALLVIQGVVPALNVYLTKATIDGLTALL
                     QGGSASLVPLVALWAGSIAISQLLAPFVQMLQGDVAELFTTKVNLDLMRKSEELKGLD
                     ALERPGFHDDLLLLRDGARNRALNVLVLSVYAARSLVAMVSLAAVLATLAWWVPFVIV
                     LASIPYGRSILKLREVGWHALLGRSPEARRMEYDSRIALDVEHAAEVRLQGLLPWLRQ
                     RYVATFASAHRAMHVARRRQALGGLPSIAFSVVVTAGLFAWAVLQASRGALSFGGIVV
                     VAQGMGQLQNEAFALNEGVGELFQRALFFEKYFEFMAIEPEVRQPDDPLPVPEGPLTV
                     AFEDVSFAYPDGRVALEHVSFAIRPGETLAIVGENGAGKSTLVKLLLRFYDPTGGRVT
                     VNGVDLRSLDVDAWRARVGAVLQAFGRYDYTLRENVTLGAAPREGEPEAVTRALTDAC
                     LG"
BASE COUNT          233 a          511 c          483 g          180 t
ORIGIN      
        1 cgccgagaca ggcgtcggtc agcgcgcgcg tgaccgcctc gggctcgccc tcgcgcggcg
       61 cggcaccgag ggtgacgttc tcccgcagcg tgtagtcgta cctcccgaac gcctgcagca
      121 cggcgcccac cctggcgcgc caggcgtcca cgtccaggct gcgcaggtcg acgccgttca
      181 ccgtcacccg cccgcccgtc gggtcgtaga agcgcaggag gagcttgacg agcgtgctct
      241 tgccagcgcc gttctcgccg acgatcgcca gcgtctcgcc cggccggatc gcgaagctca
      301 cgtgctcgag ggccacgcgc ccgtcagggt aggcgaacga cacgtcctcg aacgcaaccg
      361 tcaacggccc ctccggcacc gggagcgggt cgtccggctg cctcacctca ggctcgatgg
      421 ccatgaactc gaagtacttc tcgaagaaga gggcccgctg gaagagctca cccacgccct
      481 cgttgagtgc gaacgcctcg ttctgcagct gccccatgcc ctgcgccacc acgacgatgc
      541 cgccgaacga cagggcgccg cggctcgcct gcagcaccgc ccaggcgaag aggccggccg
      601 tcaccaccac ggagaaggcg atgctcggca ggccgccgag cgcctgccgg cgacgggcca
      661 cgtgcatcgc ccggtgcgcg ctcgcgaagg tggcgacgta gcgctgccgc agccagggca
      721 gtagcccctg gagccgcacc tcggccgcat gctccacgtc gagggctatg cggctgtcgt
      781 actccatgcg ccgcgcctcc gggctgcgac cgagcagggc gtgccagccg acctcgcgga
      841 gcttcaggat cgagcgcccg tacgggatcg aggcgaggac tatgacgaag ggcacccacc
      901 aggccagcgt ggcaaggacg gccgccaggc tgaccatggc cacgaggctg cgggcggcgt
      961 aaacggacag gacgaggacg ttgagcgccc ggttccgtgc cccgtcacgc aggaggagca
     1021 ggtcgtcgtg gaagcccggc cgctccagcg cgtcgagacc cttgagctcc tcgctcttgc
     1081 gcatgaggtc gaggttcacc ttcgtagtga agagctcggc gacgtcgccc tggagcatct
     1141 gcacgaacgg cgccagcagc tggctgatgg cgatcgagcc cgcccacagg gcgacgagcg
     1201 gcacgaggct ggcgctaccg ccctgcagga gcgccgtgag cccgtcgatg gtcgccttcg
     1261 tcaggtagac gttcaacgcc ggcacgacgc cctggatgac gagcaacgcc gtcaggagcg
     1321 tcaccttggc cggtgcggtg cgccaaagca gcgccaacga ccggagaagg atgcgccaag
     1381 cgttgaacgc gccgcccgag accttct
//
LOCUS       DAOQYE010000702         1407 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_118020_length_1407_cov_2.409763, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000702 DAOQYE010000000
VERSION     DAOQYE010000702.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1407
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118020_length_1407_cov_2.409763"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..694)
                     /locus_tag="PKN52_10330"
     CDS             complement(<1..694)
                     /locus_tag="PKN52_10330"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNR00390.1"
                     /translation="MTYRHLKLTERDGALRVALDRPEVLNALDLELLAELAAALTGPA
                     AEPRVRAVLVTGNGRAFCAGADLGSTSIDADIGAVLEEYYHPVIRSIAALEKPVVAGV
                     NGVAAGAGLSLAAACDVRIAASTATFALGFTGIGLALDAGASYFLSRLIGPGRTLELA
                     FGNRRVAAEEALRIGLVERVLDGQDFAEDVMQVVQALAAGPTLAYALVKAEVRAALDN
                     TLGEQLIVEARAQ"
     gene            complement(767..1105)
                     /locus_tag="PKN52_10335"
     CDS             complement(767..1105)
                     /locus_tag="PKN52_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNHCP domain-containing protein"
                     /protein_id="HNR00391.1"
                     /translation="MKRFTGAGANQPFECVECGANVPPLAGGGYRNHCPYCLHSLHVD
                     INPGDRANPCRGVLEPVAVEHAQKKGWVIVYRCRACGATSRNRAALDDPACPDDFALI
                     VALASGAARV"
     gene            1203..>1407
                     /locus_tag="PKN52_10340"
     CDS             1203..>1407
                     /locus_tag="PKN52_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNR00392.1"
                     /translation="MARGGAPSGRVELLRAEDLAYDQAGAELLVGVDLRLEAGDRVGL
                     VGPNGSGKSTLLGLLAGLRQPRGG"
BASE COUNT          199 a          474 c          540 g          194 t
ORIGIN      
        1 cttgcgcccg ggcctcgacg atcagctgct cgcccagggt gttgtcgagg gccgctcgca
       61 cttcagcctt gacgagcgcg tatgcgaggg ttggaccggc ggccaaggcc tggaccacct
      121 gcatgacgtc ttcggcgaag tcctggccgt cgaggacgcg ttcgacgagg ccgatgcgca
      181 gcgcctcctc ggcggccaca cggcggtttc cgaaggccag ctcgagggtc ctgcccggac
      241 cgatcagccg cgagaggaag tagcttgcgc ccgcgtcgag cgcgaggccg ataccggtga
      301 agcccagggc gaaggtggcc gtactagccg cgatgcgcac gtcgcacgcg gccgccaggc
      361 tcagaccggc gccggcggcg acgccgttga caccggcaac gaccggcttc tcgagggcgg
      421 cgatggacct gatcacgggg tggtagtact cctccagcac cgcgccgata tccgcgtcta
      481 tcgacgtgga gccgaggtcg gccccggcgc agaaagcccg accgttgccg gtgacgagca
      541 cggcgcgtac gcgcggctcc gcggcggggc ccgtgagggc ggcggcaagc tcggccagca
      601 gttccaggtc gagcgcgttg aggacttccg ggcggtcgag cgctacgcgc agggcgccgt
      661 ctcgctccgt gagcttcagg tgtcggtacg tcatgggtgg atggcggcct ccacggccag
      721 gatagcagcg ggcgacgcgc caccgtgggc ggcgcggctc ggccggctat acccgggcgg
      781 ctccactcgc caaggccacg atgagagcga agtcgtcggg gcaggcaggg tcatcgagcg
      841 ccgcccggtt acgcgacgtc gcgccgcagg cgcggcagcg gtagacgatc acccagccct
      901 tcttctgcgc gtgctcgacc gcgacgggct cgaggacccc acggcagggg ttggcgcggt
      961 cgccggggtt gatgtcgaca tggagggagt gcaggcagta ggggcagtgg ttgcggtagc
     1021 cgccccccgc caggggcggg acgttggcgc cgcactccac gcattcgaac ggctggttgg
     1081 ccccggcgcc cgtgaagcgc ttcatgagcg cagcatagcc tcgcctcacg gtagcggcgt
     1141 gaccggtcgg ctacccgccg cggctaagat tggctggtgg agccggcgat cttccagcca
     1201 gcgtggcacg cggcggcgca ccgtcggggc gcgtggaact gctgagggcc gaggacctcg
     1261 cctacgacca ggccggcgcc gagttgctgg tcggcgtcga cctgcgcctc gaggcgggtg
     1321 accgggtcgg gctcgtcggt cctaacggca gtggcaagag cacgctgctc ggcctcctgg
     1381 ccggcctgcg gcagccgcgg ggcggac
//
LOCUS       DAOQYE010000703         1406 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_118114_length_1406_cov_3.454478, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000703 DAOQYE010000000
VERSION     DAOQYE010000703.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1406
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118114_length_1406_cov_3.454478"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1195
                     /locus_tag="PKN52_10345"
     CDS             <1..1195
                     /locus_tag="PKN52_10345"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNR00393.1"
                     /translation="RRAVLAWSIVALLVLAVVAVAAVGWVLSGRVIVPSPYGLMPEFE
                     LASVEDLGGGRYAVGIPAPEQAAGRAAESPAQHANPRLRGTYAVLWEGGYAALGETLS
                     DGGGVVTRAVELLNGTPPTAGAPARLDNFYYRRDPQADLGLPFDDLTLAGPVGQLHAW
                     YVPGEQRTAVLILHGRRRGELIETLRFIEPLHEAGLPVLALAYRNHDASDASPDGLYH
                     YGASEWQDALVGALELARRGHDRVVLYGLSMGGAVALEALERWPGSAPEVVGLILDSP
                     LVSVSAVVELGAAKAGMPLPGTLTRLALLVARLRTGADFGGLEQADYAPDIGVPVLLI
                     AGEDDSTVPIASVDAFAAAVRAPLTYLRLPGVEHVEAWNHDPERYALWLREFLGSLQV
                     RAALD"
BASE COUNT          170 a          460 c          513 g          263 t
ORIGIN      
        1 gaggcgcgcc gtgctggcct ggtcgatcgt cgcgctgctc gtcctcgcgg tcgtcgccgt
       61 cgcggcggtc ggctgggtgc tctccggtcg cgtgatcgtg cccagcccgt acggtctcat
      121 gccggagttc gaacttgcca gcgtggagga cctgggcggt ggacgctacg ccgtagggat
      181 cccggcgccg gagcaggccg ccggacgggc cgccgagtcg cccgcgcagc acgccaatcc
      241 gcggctgcga ggcacgtacg ccgtgttgtg ggagggcggc tacgccgcgt taggggagac
      301 gctctccgac ggtggcggcg tcgtcacccg cgcggtggag ctgttgaacg gcacgccgcc
      361 caccgcgggc gccccggcgc ggcttgacaa cttctactac cgccgggacc cgcaggccga
      421 tctgggactg ccgttcgatg acctcaccct cgccgggccg gtcggccagc tgcacgcatg
      481 gtacgtgcct ggcgaacagc gcacggccgt cctcatcctg cacgggcggc ggcgcggtga
      541 gctgatcgag accctgcgtt tcatcgaacc gctccacgag gctggtctgc cagtcctggc
      601 gctggcctac cgcaaccacg acgccagcga cgcgagcccc gacggcctgt accactacgg
      661 tgccagcgag tggcaggacg ccctagtcgg tgcgctggag ctcgccaggc gcggtcacga
      721 cagggtagtc ctctacggac tgtcgatggg cggggcggtg gcgctcgagg cgctcgagcg
      781 ctggccgggc tcggcgcctg aggtggtcgg cctgatcctg gattcgccgc tcgtgagcgt
      841 ctccgcggtc gtcgagctcg gcgccgcgaa ggccggcatg ccgctccccg gcacgctcac
      901 ccgcctagcg ctactcgtag cgcgtttgcg cacgggcgcc gacttcggcg ggctggagca
      961 ggcggattac gcgcccgaca tcggcgtgcc ggtgctgctc atcgccggcg aggacgactc
     1021 cacagtaccc atcgcgtccg tcgacgcctt cgcggcggcc gtgcgcgcgc ctctcacgta
     1081 cttgcgcctt ccaggcgttg agcatgtgga ggcctggaac cacgacccgg agcgctacgc
     1141 gctctggctg cgcgagttcc tcggcagcct gcaggtgagg gcggcgctcg actagggcgt
     1201 gttcgttcta ggttgtgtgg tgtgatatgc agttttgcgg gtggttcgtg ttggttggtg
     1261 cgcgttgcta gtttggcatg acagtttggt agttgatgat ctccctggta gttgatgatc
     1321 tccgtaggtc ttgagcggta tcttgtgcgt ctccctagtg aggtgcccga gtacggttta
     1381 ggtggtcggg tcgagcgttg tgcttt
//
LOCUS       DAOQYE010000704         1405 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_118346_length_1405_cov_1.900741, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000704 DAOQYE010000000
VERSION     DAOQYE010000704.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1405
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118346_length_1405_cov_1.900741"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1166)
                     /locus_tag="PKN52_10350"
     CDS             complement(<1..1166)
                     /locus_tag="PKN52_10350"
                     /inference="COORDINATES: protein motif:HMM:NF037694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HNR00394.1"
                     /translation="MQRLSDGRPLDGLHVELVARSNRVLGEASTDADGHVVFPAGLAR
                     GTGNAAPALVLVEGDDDLAVLSLEEPEFDLSDRGVAGRLSPGPLDVFMTTDRGAYRPG
                     ETVNLTALARDAGARAVTGLPLTVLLLRPDGVEYARVVTDGGVAGGHVVAFALGADVP
                     RGVWRIETYADPDAQALAARSVLVEDFLPERSDFTLALGDGGPVDPAAAPDLSLEARY
                     LFGAPAAGLTLSGSVSVTTTSELAGWPGFSFGRHDQRLDTQRRMFPAGLTTDAAGRLE
                     APLPLDGLQLDARPYTLNVQALLIDGASRPVERDLTVPLRPAGPVVGIRPSFDGALAE
                     NGEAGFDLVLVAPDGAALTGELRWQADRVLTRYQWYAVDGRWFWEPVTERQRVG"
     gene            1125..>1405
                     /locus_tag="PKN52_10355"
     CDS             1125..>1405
                     /locus_tag="PKN52_10355"
                     /inference="COORDINATES: protein motif:HMM:NF024923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HNR00395.1"
                     /translation="MQAIERPAVRQALHDHVQPVGAAAQGLSGIVRARIEAPLAFQVG
                     GRIIARRVDAGQAVSAGQVLFELDPADLEQAVRAAEADAAAAETAWRTA"
BASE COUNT          198 a          498 c          542 g          167 t
ORIGIN      
        1 tcgccgacgc gttggcgctc ggtgacgggc tcccagaacc accggccgtc gacggcgtac
       61 cactggtagc gggtcagcac cctgtcggcc tgccagcgca gctcgccggt cagtgcggca
      121 ccgtcgggcg ccacgagcac caggtcgaag ccggcctcac cgttctccgc caacgccccg
      181 tcgaagctcg ggcggatgcc gacgaccggg ccggcggggc gcagggggac cgtgaggtcg
      241 cgttcgacgg gccgggaagc accgtcgatg agcagggctt ggacgttgag ggtgtagggg
      301 cgggcgtcaa gctgcaaacc atcgaggggc agcggcgcct ccaggcgccc agcggcgtcg
      361 gtcgtgaggc ccgcggggaa catgcgccgt tgggtgtcga ggcgctggtc gtggcgcccg
      421 aaggagaagc ccggccagcc ggcgagctca gaagtcgttg tcacgctgac gctgcccgag
      481 agcgtcaggc cggccgccgg ggcgccgaac aggtagcgcg cctcgaggct aaggtcgggc
      541 gccgccgccg ggtcaacggg gccgccgtcg ccgagcgcca gcgtgaagtc gctgcgctcc
      601 ggcaggaagt cctcgaccag cacgctcctg gccgccagcg cctgggcgtc gggatcggcg
      661 taggtctcta tgcgccacac gccgcgcggc acgtcggccc cgagcgcgaa cgcgaccacg
      721 tgaccgccgg cgacgccgcc gtccgttacg acgcgcgcgt actccacgcc gtccggcctg
      781 aggagcagca ccgtcagcgg caggccggtg acggcgcgcg ctccggcgtc gcgcgcgagg
      841 gccgtcaggt tgaccgtctc gccggggcgg taggcgccgc ggtccgtggt catgaacacg
      901 tcgagcgggc cgggtgagag gcggccggcg acaccgcggt ccgagaggtc gaactccggc
      961 tcctcgagcg acagcaccgc caggtcgtca tccccttcga cgagcacgag ggcgggggcg
     1021 gcgttgccgg tgccccgcgc caggccggcc gggaacacca cgtgcccgtc ggcgtcggtc
     1081 gacgcttcgc ccaacacgcg gttcgagcgc gccaccaact cgacgtgcaa gccatcgagc
     1141 ggccggccgt ccgacaggcg ctgcacgacc acgtgcagcc cgtcggtgcc gcggcacaag
     1201 gtttgtcagg gatcgtgcgt gcccgcatcg aggccccttt ggcctttcag gtcggtgggc
     1261 gcatcatcgc acgccgcgtc gacgccggac aagcggtctc cgcggggcag gtgctgttcg
     1321 agctcgatcc ggcagacctg gagcaggcgg tgcgggcggc cgaagcggac gcggcggcgg
     1381 ccgaaacggc gtggcgcacg gcgca
//
LOCUS       DAOQYE010000705         1404 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_118388_length_1404_cov_3.555967, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000705 DAOQYE010000000
VERSION     DAOQYE010000705.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1404
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118388_length_1404_cov_3.555967"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="PKN52_10360"
     CDS             complement(<1..240)
                     /locus_tag="PKN52_10360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00396.1"
                     /translation="MKSHQVRTYKSAEHLPREEQLAWKLAAVATDPVPLEPAVLEMIG
                     NRIIDNASVAVASLTRRPVVAARSQALVHPRTPGAA"
     gene            417..905
                     /locus_tag="PKN52_10365"
     CDS             417..905
                     /locus_tag="PKN52_10365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00397.1"
                     /translation="MNLTSHRRVLTPLALLCVLAIFGNANAQNWFGVRSGYPLGVTLH
                     YGIANALSNGFDLRVSGRVTASGGSAHFGIGVDAMTTVAAEGPFTVYLGAGPAIEFGN
                     GGAWLDIHALAGGEFRFVDFGLAPLGLFLEATLGGSFGLGNGSPARIPTFGAALGVNY
                     HF"
     gene            905..>1404
                     /locus_tag="PKN52_10370"
     CDS             905..>1404
                     /locus_tag="PKN52_10370"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNR00398.1"
                     /translation="MAERRFGVAVIGAGNIAAAHIAAVAGLPQGRLVGVAGRTPEKPR
                     ALAEKHGVKAYASVAEAVADPEVEVVAVCTPSGAHLEPAMAAVAAGKHVIVEKPLEIS
                     TERARWLIRAAEDAGVALATVFMSRFADANAYVKQAIDAGRLGTLLQGNAYVPWYRTQ
                     AYYDSGA"
BASE COUNT          198 a          528 c          478 g          200 t
ORIGIN      
        1 ggccgcgccg ggcgtgcgcg gatggaccag cgcctgcgag cgcgcagcga cgaccgggcg
       61 cctggtcagc gacgcgacgg ccaccgacgc gttgtcaatg atacggttgc cgatcatctc
      121 caggacggcc ggctccagcg ggacggggtc ggtggccacc gccgcgagct tccaggcgag
      181 ctgctcctcg cgcggcaggt gctcggcgga cttgtaagtg cgtacctgat gggacttcat
      241 cgtctcaagg acgatagcat ccgcccggac gggcatactg cctcgcgacg ggcggtgcgg
      301 aagaccgggc cgcccggccc aaactgggcg ccgaccgggc cgtataccag ccccgctcag
      361 cccatacgtg gaactcctcg caccgcctca tgaacgcatc cggaagaatg gctgccatga
      421 acctcacctc ccaccgtcgg gtgctcaccc cccttgccct cctctgcgtc ctcgccatct
      481 tcggcaacgc caacgcccag aactggttcg gcgtcaggag cggctacccg ctcggcgtca
      541 ccctccacta cggcatcgcc aacgccctct ccaacggctt cgacctgcgc gtcagcggcc
      601 gcgtgacggc cagcggcggc agcgcccact tcggcatcgg cgtcgacgcg atgacgaccg
      661 tggcggccga aggtccgttc accgtctacc tcggggccgg cccggccatc gagttcggca
      721 acggcggcgc gtggctcgac atccacgcct tggccggcgg cgagttccgc ttcgtcgact
      781 tcggcctcgc gcccttgggc ctcttcctcg aagccacgct gggcggctcc ttcggcctcg
      841 gcaacggctc gccggcgcgc atcccgacgt tcggcgcggc cctcggcgtc aactaccact
      901 tctgatggcc gagcgccgct tcggcgttgc cgtcatcggc gcaggcaaca tcgcggcggc
      961 gcacatcgcc gccgtggccg gcctgccgca ggggcggctc gtcggcgtgg caggccgcac
     1021 gccggagaag ccccgcgcgc tggccgagaa gcacggcgtg aaggcgtacg cctcggtggc
     1081 tgaggccgta gccgacccgg aggtagaggt cgtggcggtc tgcacgccgt ccggcgccca
     1141 ccttgagccg gccatggccg cggtggcggc cggcaagcac gtcatcgtcg agaagccgct
     1201 cgagatctcg accgagcgcg cccggtggct catccgggcc gccgaggacg ccggcgttgc
     1261 gctcgccacc gtgttcatga gccgcttcgc cgacgcgaac gcgtacgtca agcaggcgat
     1321 cgacgccggt cggctcggca cgctgctgca gggcaacgcg tacgtgccgt ggtaccgcac
     1381 gcaggcgtac tacgacagcg gcgc
//
LOCUS       DAOQYE010000706         1401 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_118841_length_1401_cov_3.185736, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000706 DAOQYE010000000
VERSION     DAOQYE010000706.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1401
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118841_length_1401_cov_3.185736"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            450..1388
                     /locus_tag="PKN52_10375"
     CDS             450..1388
                     /locus_tag="PKN52_10375"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HNR00399.1"
                     /translation="MRFLKALLLIAIVLAAAYGVLALVNGPRGPHPYYSRLGAGPLVL
                     AHGGGQGLWPDNTMRAFTGSAALGADVLELDVQRDGDGAFRVIHDATVDRTTDGTGLV
                     SALRAAQVEALDDGYRWTVSGNRPSGQDGDFPYRGQGVAIPTLAEVLAGFPDSAVNIE
                     IKEEVSEAGTALCELLRRTGSTGRVLVASFNSAPMKAFRQACPEVATSATRSEVTLFY
                     VLARARLSAVYTPPFVAVQVPVQQGSLTVVTPHFIQAAHARGVKVEVWTVNESAEMRR
                     LLDMGVDGLITDRPDRAMAVLGRRFDAGIVPDFVLP"
BASE COUNT          209 a          497 c          463 g          232 t
ORIGIN      
        1 cgttgcctcc atggccgcgt taaccaccgg tggagaagac gtacccccct gggcgttggc
       61 gaacgagaga ccgacggtga ggattgctga tagtacgcga cgatgtccag gcacctgtcc
      121 ttcactcctt cccacacgtt cgaacctatt agttggcctg gaagcggact gaggataatg
      181 ccgcgatggg ccaccatggt tggcgggcgc gtgacaggcg ccaatgatac agccgtactc
      241 atcaggaccg gcgcttcgtg gggcacggag cggtagcgag atcggttccg agcgtggtct
      301 ggaacgtagg aatggcggtg cgcgtccacg gtctcggcgc ggcgcctggc gccttgcgca
      361 caggctcgct acgattcgcg catagactct ctagctagag gccacgccaa ccaagcccca
      421 gcccctcgct ccggatagga ccggtgacaa tgcgcttcct caaggccctc ctgctcatcg
      481 ctatcgtcct cgcggccgcc tacggcgtcc tggccctagt caacggcccg cgcgggccgc
      541 atccttacta ctcgcgcctc ggagcgggtc cgctcgtgct cgcgcacggc ggcggtcagg
      601 gcctgtggcc ggacaacacc atgcgcgcct tcacgggttc cgccgcgctt ggggcagacg
      661 tgctcgagct cgacgttcaa cgcgacggcg acggcgcttt cagggtgatc cacgacgcca
      721 ccgtcgaccg cacgacggac ggcactgggc tcgtctccgc cctgcgcgct gcccaggtcg
      781 aggccctcga cgacggctac cgctggaccg tctccggcaa ccgcccgtcc ggtcaggacg
      841 gcgacttccc gtaccgcggg cagggggtcg ccatcccgac cctcgccgag gtgctcgcgg
      901 gcttccccga ctcggccgtc aacatcgaga tcaaggagga ggtctccgag gcaggcacgg
      961 ccctctgcga gttgctccgc cgcaccggca gcaccggccg cgtcctcgtg gcctcgttca
     1021 acagcgcccc gatgaaggcc ttcaggcagg cttgtccgga ggtcgccacc tccgccacgc
     1081 gctcggaagt gacgctcttc tacgtgctgg cgcgggctcg cctcagtgcc gtctacacgc
     1141 cgccgttcgt cgccgtccag gtgcccgtgc agcaggggag cctcacggtc gtgaccccgc
     1201 acttcatcca ggcggcgcac gcgcgcgggg tgaaggtcga ggtgtggacc gtcaacgagt
     1261 cggccgagat gcgccgcctg ctcgacatgg gcgtcgacgg tctcatcacc gaccggccgg
     1321 atcgggccat ggcggtgctc gggcggcggt tcgacgccgg gatagtcccg gacttcgtac
     1381 tgccatgacc gttctcgctg g
//
LOCUS       DAOQYE010000707         1399 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_119203_length_1399_cov_2.165179, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000707 DAOQYE010000000
VERSION     DAOQYE010000707.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1399
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119203_length_1399_cov_2.165179"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..337)
                     /locus_tag="PKN52_10380"
     CDS             complement(<1..337)
                     /locus_tag="PKN52_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HNR00400.1"
                     /translation="MALEFYNTLTREKQPFRPVVPGHVGIYMCGPTVYSEPHLGHARG
                     PVVFDVLRNWLEHEGFVVRLVTNVTDVGHLVDDADDGEDKLARRAQIERLEPMEVAEK
                     YFWAYFEAMA"
     gene            629..1243
                     /locus_tag="PKN52_10385"
     CDS             629..1243
                     /locus_tag="PKN52_10385"
                     /inference="COORDINATES: protein motif:HMM:NF013378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HNR00401.1"
                     /translation="MAHVTVANAANLEQLVQGSRFLASVFEIETPQHAADRLADIRRE
                     HPDATHHCWAFRVGANQRFSDDGEPGGTAGRPMLEVILKRGLDHVGAVVVRYYGGKKL
                     GAGGLVRAYSGSVAKALDLAGTREVVDMARIRVAAPFAGTDVVLRLLDEVARLDAFQP
                     LARRSPGFGADGMTVEVSLPAARLGELEARLREATNGGATLELA"
BASE COUNT          233 a          463 c          497 g          206 t
ORIGIN      
        1 cggccatggc ctcgaagtag gcccagaagt acttctccgc gacctccatc ggctcgagcc
       61 gctcgatctg cgcgcggcgc gcgagcttgt cctcgccgtc gtcagcgtcg tcgacgagat
      121 ggccgacatc cgtgacgttc gtaacgagcc tcacgacgaa cccctcgtgc tcgagccagt
      181 tgcgcagcac gtcgaagacg accggaccgc gagcgtgccc gaggtgcggt tccgaataca
      241 cggtaggacc gcacatgtag atgccgacgt gaccaggcac gaccggcctg aacggctgtt
      301 tctcgcgcgt gagggtgtta tagaactcga gcgccatgac gcttgctcct tcgtgttacg
      361 cctcgaccgg ctcccaagta gagcctgcga gggtggctgg ttacctaagc ggaaaggtac
      421 cgcaccgcag cggcggcacg gttgaaggtc ctagagcagt gacgcacgac atcgcggcgc
      481 gcgggcgacg acgagcacct cgccccggag cgtagcgacg cgcgcacgta aggagccgcc
      541 tgcggcgggt aggaaagtgt gcgcgctcat gcggcggagt ctagcatgca cgccatcggc
      601 cgcagaccgc cccgcggtac agtcctacat ggcgcacgtc acggtcgcga acgctgccaa
      661 cctcgagcag ctcgttcagg gttcgcgttt cctggcgtcc gtgttcgaga tcgagacccc
      721 gcaacacgcc gccgaccgcc tggcggacat ccggcgggaa caccccgacg cgacccacca
      781 ctgttgggcg ttccgagtcg gcgccaacca acgcttctca gacgacgggg agccaggcgg
      841 cacggccggc agacccatgc tggaggtcat cctcaaacgc gggctcgatc atgtcggcgc
      901 ggtggtggtc aggtattacg gaggcaagaa gctcggcgcc ggcgggctcg ttagggcgta
      961 ctcgggtagc gtggcgaagg cgctcgacct ggccggcacg cgcgaggtcg tcgatatggc
     1021 gcggatccgc gtggcagcgc cgttcgcggg gaccgacgtg gtgctgcggc tcctggacga
     1081 ggtcgcgagg ctcgacgcct tccagccgct cgccaggcgc tcgcccggct tcggagccga
     1141 cgggatgacc gtggaggtca gtctgcccgc cgccagactg ggggagttgg aggcgcggct
     1201 cagggaggcg accaacgggg gcgcgacgct cgagctcgca taggcgcgcg tcgggcggcc
     1261 ggagcggccg gcggtccgtc taacctcgcc atgaagccgc cctcaccgca ccgagggccg
     1321 gaaggtggca cgatagggac atgatgagac ggcgccgcac gacctggttg gcagtcctgt
     1381 tgctcggcgc gctcctagg
//
LOCUS       DAOQYE010000708         1398 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_119299_length_1398_cov_2.964259, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000708 DAOQYE010000000
VERSION     DAOQYE010000708.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1398
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119299_length_1398_cov_2.964259"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(135..590)
                     /locus_tag="PKN52_10390"
     CDS             complement(135..590)
                     /locus_tag="PKN52_10390"
                     /inference="COORDINATES: protein motif:HMM:NF025734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding domain-containing protein"
                     /protein_id="HNR00402.1"
                     /translation="MTRWKTRALLGTVVVLGVMQLIAVDRSNPPVEEEMPASPEVREV
                     LRRACYDCHSNETVWPWYAHVAPVSFLVARDVNEGRRHLNFSTWNQRSERRRARAYEE
                     IREQVDEGEMPLWFYVPLHPEAKLTAADQTLLHAWTVAQEATAPTADGP"
     gene            734..1222
                     /locus_tag="PKN52_10395"
     CDS             734..1222
                     /locus_tag="PKN52_10395"
                     /inference="COORDINATES: protein motif:HMM:NF012436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HNR00403.1"
                     /translation="MLISAALTQAINAQIGNELGACLQYHQIANHFQRAHLTLLADLF
                     FKQAAEEREHAEKLLRYIVDTGGELRLPAVREPVHTFASAEAAVEAALAWELEVTGQI
                     KGLMDLAASENDYLAQGFLQWFIDEQREEVMKMQRLLGVVRMAGERNLLSVEAYLAHG
                     GD"
BASE COUNT          221 a          511 c          464 g          202 t
ORIGIN      
        1 gcgtggcgac aacgtgcacg agaccggacc acgcgagtgg caggagaaga tcggccgcgg
       61 gctgccggtg ctcgccggct gagcaagacg cgacgcgcgg ctcgcctcag gcgccgcgcg
      121 tcgctcggcc gacatcaggg accgtccgcc gtcggcgcgg tggcctcctg ggccacggtc
      181 cacgcgtgca gcagcgtctg atccgccgcc gtcagctttg cctccgggtg cagcggcacg
      241 tagaaccaga gcggcatctc gccctcgtcc acctgctcgc ggatctcctc gtaggcgcgc
      301 gcgcggcgac gttccgagcg ctggttccac gtcgagaagt tgaggtgccg ccgaccctcg
      361 ttgacatcgc gcgccaccag gaacgacacc ggggccacgt gcgcgtacca cggccagacc
      421 gtttcgttgc tatggcagtc gtagcaggcg cgtcgcagca cctcgcgcac ctcgggggac
      481 gccggcatct cctcctcgac cggcggattc gatcgatcga cggcaatcag ctgcatgacg
      541 cccagcacca cgacggtgcc cagcagcgcg cgggtcttcc agcgagtcat cagcagggtt
      601 cctcggcacg gagcgacgat ggcgcgcgta cggtgacgcg cgccggcgtg tcatgggtga
      661 cgtcgaaccg tccgcgacgt taactctcag ccggtcgaag ccgtcccgcg tcccctcgca
      721 cccggaagcc cgtatgctca tcagcgccgc cctcacgcag gccatcaacg cccagatcgg
      781 caacgaactc ggcgcctgcc tgcagtacca ccagatcgcc aaccacttcc agcgcgcgca
      841 cctcacgctg ctcgccgacc tgttcttcaa gcaggcggcc gaagagcggg agcacgcgga
      901 gaagctgctc aggtacatcg tcgacacggg tggtgagctg cgtctgcccg cggtgcgtga
      961 accggtgcat acgttcgcct ccgccgaagc ggccgtcgaa gccgcgctcg cctgggagct
     1021 cgaggtgacg ggacagatca agggcctgat ggacctcgcc gcgtcggaga acgactacct
     1081 cgcgcagggc ttcctgcagt ggttcatcga cgagcagcgc gaagaggtca tgaagatgca
     1141 gcgcctgctt ggcgtcgtgc gcatggcggg cgagcgcaac ctgctctcgg tcgaagcgta
     1201 cctcgcccac ggcggcgact gagcggcatg cgaggcgtcg ggcccgctgg tggtcgcgcg
     1261 atcacctccc cgtgtccgac gccgcgcgac gtcaggggcg cgcgatcacg cgccccgcgc
     1321 gcacacccgt cgtccgcccg tcctccacgg ccacctcgcc gttcaccagc accgtgcgaa
     1381 ttcccaccgg gtactggt
//
LOCUS       DAOQYE010000709         1397 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_119464_length_1397_cov_2.649776, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000709 DAOQYE010000000
VERSION     DAOQYE010000709.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1397
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119464_length_1397_cov_2.649776"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1397
                     /locus_tag="PKN52_10400"
     CDS             <1..>1397
                     /locus_tag="PKN52_10400"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNR00404.1"
                     /translation="AKVTAAAAQTPSVASLGPAGVAASGLPGARSDDLAVAAELDPGS
                     AWVVVEYARTVALDGDLATASAAAKRAVELAPDDAEVQATAGVVFDAAGDGAAAEAAF
                     QAAIAANPAHAVALAGRAGSGSSADASADLQAAIAAYPRFVDAYVRLAGLQSDSQRAL
                     QTLRRAESYSPESVLLRGSVMRRLVSAGAGGDALAYLQQSLTDPLARSAGLYALARLL
                     PSAYVDQALRLVTDGQEAYPDSVDLKVAQADLLLQAGRAEEAEAVLGPLFTQYPTDPS
                     VGNLLAVAQARRGDLAGAKRTFEALRGTGADVDKSLAELYLAAGRAAGALGLVEPLVT
                     ADPENAELLALYGTALVRVGRLADGRQALEKAVAIDPEQAIARRSLDLLGQQSQLTGG
                     AEVTFSEEAGVAFQQGLYALDVSDYAAAVAAFSRSNEVQATGLASFYLGYSRQLNGDT
                     RGAVSDYEAALQTYP"
BASE COUNT          180 a          564 c          470 g          183 t
ORIGIN      
        1 cgccaaggtc acggccgcgg ccgcccagac cccgagcgtg gcgagccttg gtcctgcagg
       61 cgtcgccgcg tccgggctgc cgggcgcccg ttccgacgac ttggcggtgg cggccgagct
      121 cgaccctggc tcggcgtggg tggtcgtcga gtacgcccgt accgtcgccc tcgacggcga
      181 ccttgccacg gccagcgccg cggccaagcg cgctgtcgag ctcgcgcccg acgatgccga
      241 agtccaggcg actgccggtg tcgtgttcga cgcggccggt gacggcgccg cggccgaggc
      301 ggcgttccag gccgccatcg ccgccaaccc cgcccatgcc gtggcgctcg ccggtcgcgc
      361 cggatcaggc tcgagcgccg atgccagcgc cgacctgcag gccgccatcg cggcctaccc
      421 gcgcttcgtg gacgcctacg tgcgcctcgc aggcctgcag agcgactccc agcgcgcgct
      481 ccagacgctc aggcgcgccg agagctactc ccccgagtcc gtgctcctgc gcggctccgt
      541 catgcgccgc ctggtaagcg ccggcgcggg cggcgacgcg ctcgcctacc tgcagcagtc
      601 cctcaccgat cccctcgccc gctccgccgg tctctacgcc ctggcgcggc tgctaccgtc
      661 cgcttacgtg gaccaggctt tgcgcctcgt gaccgacgga caggaggcgt accccgacag
      721 cgtcgacctc aaggtcgcgc aagccgacct cctcctgcaa gcgggcaggg ccgaagaggc
      781 cgaagcggtc ctcggaccgc tgttcaccca gtaccccacc gacccgtccg tcggcaacct
      841 ccttgccgta gcccaggcgc ggcgcggcga cctggccggc gcgaagcgca cgttcgaggc
      901 cctgcgcggc accggcgccg acgtcgacaa gagcctggcg gagctgtacc tggccgccgg
      961 ccgcgcggcc ggcgctctcg gcctcgtcga gccgctggtg accgccgacc cggagaacgc
     1021 cgagctgctg gccctgtacg gcacggccct cgtacgcgtc ggtcgcctcg ccgatggccg
     1081 gcaggctctc gagaaggccg tagccatcga ccctgagcag gccatcgcgc gccgcagcct
     1141 cgacctcctc ggtcagcaga gccagctcac cggcggggcg gaagtgacgt tctccgagga
     1201 ggccggcgtg gcgttccagc agggcctgta cgcgctcgac gtcagcgact acgccgccgc
     1261 cgtcgccgcg ttctcgcgct ccaacgaggt acaggccacc ggcctcgcgt ccttctacct
     1321 cggctactcc aggcagctca acggcgacac gcgcggcgcc gtctccgact acgaggcagc
     1381 gctccagacc tacccga
//
LOCUS       DAOQYE010000710         1394 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_119884_length_1394_cov_2.447349, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000710 DAOQYE010000000
VERSION     DAOQYE010000710.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119884_length_1394_cov_2.447349"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1096)
                     /locus_tag="PKN52_10405"
     CDS             complement(<1..1096)
                     /locus_tag="PKN52_10405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00405.1"
                     /translation="MPRHRTGTVRSPVLIGILTAALCLVLGACGGPTSDPVPLGAARV
                     TGRLALPAGHSLDVASLAVTTPFGVYPLGPDGRFSAYVMKGTSSELAVSTPAGDLVAL
                     GVTEGADAQLSLRSTSEALLYYLVGGMLLPADQQDVVRSLLSERPEVADVMAHLQRLL
                     LAGGNGLDAPDQAMRDAMLAAQAGIVGDPEVQAALQRQAVCPFAPWLLNAHGTELQPQ
                     AGEDSVIIQGGTDMQAGAMVLHNPAGRGVVVQNHLRRPAALLAYEVAWEDVDQASHEV
                     DPPVLVGTVDVPATGNLEFFAALGDIVTGDAPWAPVLSEPLVLSSHAEATLTHYELVL
                     IGPSLTSDKLPIWNDSRFTSFHDDWDDIAFD"
BASE COUNT          207 a          487 c          498 g          202 t
ORIGIN      
        1 tgtcgaacgc gatgtcgtcc caatcgtcat ggaacgacgt gaaccgcgag tcattccaga
       61 tcggcaactt gtcactcgtg aggctggggc cgatgagcac gagctcgtag tgcgtgagcg
      121 ttgcctccgc gtgcgacgac agcacgagcg gctcgctcag cactggggcc cacggggcgt
      181 cgccggtgac gatgtcgccc agggccgcga agaactcgag gttgccggta gcgggcacgt
      241 cgaccgtgcc tacgaggacg ggcgggtcga cctcatgcga agcctggtcc acgtcctccc
      301 atgccacctc gtacgccagg agcgcggccg ggcgcctgag gtggttctgg acgaccacgc
      361 ccctgcccgc cgggttgtga agcaccatgg cgcccgcctg catgtcggtg cccccttgga
      421 tgatcacgct gtcctcaccg gcctgaggct gcagctcggt gccgtgcgcg ttcagcaacc
      481 acggcgcgaa cgggcacacc gcctgcctct ggagggccgc ctgcacctcc gggtcgccga
      541 cgatgcctgc ctgcgccgcg agcatcgcgt cgcgcatggc ctggtcgggg gcatccagcc
      601 cgttgccgcc cgccagtagg aggcgttgga ggtgcgccat gacgtcggcc acctctggtc
      661 tctcgctcag caggctccgt acgacgtcct gttggtcggc tggcagcaac atgcccccga
      721 cgaggtagta caggagcgcc tccgacgtgc tccggagcga gagctgggcg tcggcgccct
      781 cggtgacccc caacgccacg aggtcgcccg cgggcgtcga gaccgctagt tcgctgctgg
      841 tgcccttcat gacgtacgcg gagaaccgcc cgtccggccc caaggggtag acgccgaagg
      901 gcgtggtgac cgcgagcgac gcgacgtcaa gggagtggcc cgccggcagc gccaacctgc
      961 cggtgacgcg cgcggccccc aacgggaccg ggtcgctggt cggaccgccg caggcgccca
     1021 gaaccaggca gagggccgcg gtgaggatgc cgatgagcac gggcgagcgg acggttccgg
     1081 tgcggtgtcg gggcatatgc cattgtggaa cccgttcggt taaagcggcg ttaaatgagc
     1141 acgggcgcgt ggggcacgcg gctttcgacc ccggcttcag gtggggctta tcaagcggtc
     1201 cgcttgtcaa gcgttgtcaa gcgctccgct tctcgaccgg ctccgaccat tccgccaggc
     1261 gccggctcag cgcgcccatg gccgcccccg gcgtgagcct caaggcggcg tcgcggaggc
     1321 cgacggccac agggccggag gcctgcgcga cacggccggc caacacctgc acggccgcgc
     1381 gacgcgcggc tgcc
//
LOCUS       DAOQYE010000711         1391 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_120281_length_1391_cov_2.891467, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000711 DAOQYE010000000
VERSION     DAOQYE010000711.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1391
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120281_length_1391_cov_2.891467"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1391)
                     /locus_tag="PKN52_10410"
     CDS             complement(<1..>1391)
                     /locus_tag="PKN52_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNR00406.1"
                     /translation="GDQPEAIANLVGGIEDGLRFQTLLGATGTGKTYTMAKVIEAVNR
                     PALVLAPNKVLTAQLASEFRDFFPDAAVEFFISYYDYYQPEAYVPARDLFIEKDSNVN
                     MELERLRHSTTRSLLTRRDVIVVASVSAIYGLGSPEEYEKLHLLLDVGKRLPRDEILA
                     TLVRQQYERNDIELAAGRFRARGDVVEVWAAYEERPLRIELWGDEIDRLTMIDPVTGE
                     EVAELEHAMVFPAKHYVTPYEKLAPAVEEIEHDLVERLAYFEATGRYLEKQRLKERTN
                     YDLEMLRTLGYCQGIENYSRYLERRPAGSTPGTLLDYFPDDFVTFLDESHVMLPQVRG
                     MFNGDRNRKQTLVDYGFRLPAALDNRPLKEEEFLSKVGQVVFVSATPTERELGMSDQV
                     VEQVIRPTGLVDPLVTVKPSRGQIDDLLFAVRERAKKNERVLVTTLTKRMAEDLTEYL
                     ATQGARVRYMHSD"
BASE COUNT          208 a          440 c          494 g          249 t
ORIGIN      
        1 gtcggagtgc atgtaccgca cccgcgcgcc ctgcgtggcg aggtactccg tgaggtcctc
       61 ggccatgcgc ttggtcagag tggtgacgag gacgcgctcg ttcttcttgg cgcgctcgcg
      121 cacggcgaag agcaggtcgt cgatctggcc gcggctcggc ttgaccgtaa cgagcggatc
      181 gacgaggccg gtggggcgga tgacctgctc gacgacctgg tcggacatgc cgagctcgcg
      241 ctccgtcggc gtggcgctca cgaacacgac ctgcccgacc ttcgagagga actcttcctc
      301 cttgagcggt cggttgtcca gcgccgccgg tagcctgaag ccgtagtcga cgagggtctg
      361 cttgcggttg cggtcgccgt tgaacatgcc gcggacctgc ggcagcatca catgcgactc
      421 gtccaggaac gtcacgaagt cgtccgggaa gtagtcgagc agggtgccgg gcgtgctgcc
      481 ggccggacgc cgctccaggt agcgcgagta gttctcgatg ccttggcagt agccgagcgt
      541 gcgcagcatc tcgaggtcgt agttggtgcg ctccttgagc cgctgcttct cgaggtacct
      601 gccggtcgcc tcgaagtagg ccaggcgctc caccaggtcg tgctcgatct cttcgacggc
      661 gggggcgagc ttctcgtacg gtgtgacgta gtgcttggcg gggaagacca tggcgtgctc
      721 tagctccgcc acctcctcgc ccgtgaccgg gtcgatcatg gtgaggcggt cgatctcgtc
      781 gccccacagc tcgatgcgca gcggccgctc ctcgtacgcc gcccagacct ccaccacgtc
      841 gccacgggca cggaagcggc ccgccgcgag ctcgatgtcg ttgcgctcgt actgctggcg
      901 gaccaacgtc gcgaggatct cgtcgcgcgg aaggcgcttg ccgacgtcga gcagcaggtg
      961 gagcttctcg tactcctccg gcgagcccaa gccgtagatg gccgacacgg aggcgacgac
     1021 gatgacgtca cgccgcgtga gcagcgagcg cgtcgtggag tgccgcaggc gctcgagctc
     1081 catgttgacg ttcgagtcct tctcgatgaa gaggtcgcgg gccgggacgt aggcttccgg
     1141 ctggtagtag tcgtagtagg agatgaagaa ctcgacggcg gcgtcgggga agaagtcgcg
     1201 gaactcggag gccagctgcg cggtcaggac cttgttgggc gccaggacga gagcgggccg
     1261 gttgaccgcc tcgatgacct tggccatcgt gtaagtcttg cccgttccgg tggcgccgag
     1321 gagggtctgg aagcgcagcc cgtcctcgat cccgccgacc aggttggcta tcgcctcggg
     1381 ctgatcgccc t
//
LOCUS       DAOQYE010000712         1390 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_120448_length_1390_cov_2.360300, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000712 DAOQYE010000000
VERSION     DAOQYE010000712.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1390
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120448_length_1390_cov_2.360300"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="PKN52_10415"
     CDS             complement(<1..425)
                     /locus_tag="PKN52_10415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00407.1"
                     /translation="MAWIESHQSLRDHPKKDALCERLFDGSVPDDVADFATIGLLHQI
                     WWWCLTYAIDGDLANFSDRQIARGCRWGGDAQTLVRALIASGFMDEDRKLHDWHDYAG
                     RMLTLKERARKANAQRQQRYRERHPQSPAEAVTRNVTRNV"
     gene            complement(425..973)
                     /locus_tag="PKN52_10420"
     CDS             complement(425..973)
                     /locus_tag="PKN52_10420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00408.1"
                     /translation="MTEPLRYADLSDPNDWLHGSDLAGKAHTLTITAVSVELFADDAR
                     ADAKTRRQGVLEFAKTQKRLGLNRTNRECLRAMFGPDDPSQDVVPLWTGKRVTFFPSP
                     ERNPATGKVEPAVRIKGSPDIAAAITFTLNLPRRKPRQVTLVRTGPAAVKEAEPAKEA
                     EPDDEPDFGEPEWMRDDEGELG"
     gene            complement(970..>1390)
                     /locus_tag="PKN52_10425"
     CDS             complement(970..>1390)
                     /locus_tag="PKN52_10425"
                     /inference="COORDINATES: protein motif:HMM:NF024096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease-like domain-containing
                     protein"
                     /protein_id="HNR00409.1"
                     /translation="PDALIDLKTSADIEAQHFGRTAARLLYHGQLAYYHAGACSCAGL
                     VDLPVRIIAVEAEPPHDVCVYEVSDDDLWAGMELVQDLLAQLRRCRDTDSWLGRYVGP
                     QPLWLPGWVFAEGGGDDFDLQVGPQSAYVLANTGGLP"
BASE COUNT          209 a          465 c          467 g          249 t
ORIGIN      
        1 acgttacgcg ttacgttacg cgttacggcc tctgccggac tctgcgggtg gcgctcccgg
       61 taacgttgct ggcgctgcgc gttagccttc ctggcccgct ctttgagcgt cagcatacgg
      121 ccagcgtagt cgtgccagtc gtgcagcttc cgatcctcgt ccatgaagcc tgacgctatg
      181 agagcccgta ccagcgtctg tgcatcgccc ccccaccgac agccgcgagc tatctggcga
      241 tcgctgaagt tcgcgaggtc gccgtcgatg gcataggtca ggcaccacca ccagatctgg
      301 tgcaagagcc cgatcgtggc gaagtcggcc acgtcgtcgg gcaccgagcc gtcgaacagc
      361 ctctcgcaca gggcatcctt cttcgggtgg tcgcgcagcg attggtggga ctcgatccag
      421 gccatcagcc cagctcgccc tcgtcatcgc gcatccattc aggctcgccg aagtccggtt
      481 cgtcatccgg ctccgcttcc ttggccggct ccgcctcctt gacggcagcc ggacccgtcc
      541 gaacgagcgt gacctggcgc ggcttccggc gcggcaggtt gagcgtgaag gtgatcgccg
      601 ccgcgatgtc gggcgagccc ttgatccgga ccgcgggctc caccttgccg gtcgccgggt
      661 tgcgctccgg cgacgggaag aaggtcacgc gcttgcctgt ccagagcggc accacgtcct
      721 gcgacgggtc gtcgggaccg aacatggctc tgaggcattc gcggttggtc cggttgaggc
      781 ctaacctctt ctgcgtcttg gcgaactcga gcacaccctg gcgccgggtc ttggcgtcgg
      841 ccctggcgtc gtcggcgaag agttctaccg agacggccgt gatggtgagc gtgtgcgcct
      901 tgccggcgag gtcgctgccg tgcagccagt cgttcgggtc ggagagatcg gcgtatcgca
      961 gcggctcggt catggcagac ctcccgtgtt ggcgagcacg taggccgact gcggcccgac
     1021 ctgcaggtcg aagtcatcgc cgccgccctc cgcgaatacc cagcccggca accacagcgg
     1081 ctgcggtccg acgtagcgcc cgagccatga gtctgtatcg cggcagcgac ggagctgggc
     1141 gagcaggtcc tggaccagct ccatgccagc ccacaggtcg tcatccgata cctcatacac
     1201 gcacacgtca tgcggcggct cggcctcgac ggcgatgatg cgcaccggca ggtcgacaag
     1261 tccggcacat gagcaggcgc cggcgtggta gtaggcgagc tggccgtggt agagcaggcg
     1321 cgcagcggta cggccgaagt gctgggcttc gatgtcggcc gatgtcttga ggtctatcag
     1381 agcgtcaggc
//
LOCUS       DAOQYE010000713         1389 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_120584_length_1389_cov_2.844828, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000713 DAOQYE010000000
VERSION     DAOQYE010000713.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120584_length_1389_cov_2.844828"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /locus_tag="PKN52_10430"
     CDS             complement(<1..551)
                     /locus_tag="PKN52_10430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="E3 binding domain-containing protein"
                     /protein_id="HNR00410.1"
                     /translation="MSEPEIARLARNLAEQNNVDWRRLSGTGPGGLILERDVLDFLAR
                     VMAGEEALDPTPEPLPDGMDEWPEVHADSVGNYARPSSAQRSYADGADAAADAPAEEA
                     DDAAFAELGEDVFLFDDDELDEEAEPAAAAFAEADAFAKADDYAESVRVADAALSVDA
                     EEERGGDAGEEDVLLVADEPAVA"
     gene            complement(742..>1389)
                     /locus_tag="PKN52_10435"
     CDS             complement(742..>1389)
                     /locus_tag="PKN52_10435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00411.1"
                     /translation="VSFSQPRAVVSMSPAAGEVVPPGTPVLLSYSLSTANVVQVPDVM
                     GQPQWQAQLAVLAAQLRIGQITFVQDPDRPEGVVDVRPTGFTVPGTPVFLTINGVSSG
                     PQPDFTLPDTTLPGDTGGGVRPPIDGAPTGGDATGDGSRLVPFTFDPTNMGVKRLLEQ
                     PYQLKLVVSDDRGERVVFDRRVAAGQSVVTSVAIYGQEAMLQTYVDDVFFQAWRP"
BASE COUNT          201 a          493 c          479 g          216 t
ORIGIN      
        1 tccgccaccg ccggctcgtc ggccacgagc agcacgtcct cctcgccggc atcgcctccg
       61 cgctcctcct cggcgtcgac ggacagcgcg gcgtcggcca cccgaaccga ctcggcgtag
      121 tcgtcggcct tcgcgaaggc gtccgcctct gcgaaggcgg ccgccgccgg ctccgcttcc
      181 tcgtcgagct cgtcgtcgtc gaacaggaag acgtcctcac cgagctcggc aaaggcagcg
      241 tcgtcggcct cttccgctgg ggcgtctgcg gccgcgtcgg ccccgtcggc gtaagaccgc
      301 tgggcggagc tagggcgcgc gtagttgcct accgagtcgg catggacctc cggccactcg
      361 tccatcccat cggggagggg ctcaggggtc ggatcgaggg cctcctcgcc ggccatgacg
      421 cgggccaaga aatcgagcac gtcgcgctcg aggatcaacc ctcccgggcc cgtgccgctg
      481 aggcggcgcc aatccacgtt gttctgctct gccaggttcc tggccagtct tgcgatctca
      541 ggttcgctca cgagcgttcc tcccgggcgg tatcaccgtt ggctgcggca ggcggcctgc
      601 gcatgagcga gcgcgtgctg gtcacctgag gggcgcgaag gtggctcaga gtatagcgtg
      661 ccgccctacg cgacggttcc ggcaatttgc cacttttgcg cgctgggcag cttcgcgagc
      721 gacccggctc gcacaagcgg ctcacggacg ccaggcctgg aagaacacgt cgtcgacgta
      781 agtctggagc atggcctcct ggccgtagat ggcgacggac gtaacgacgc tctgcccggc
      841 ggcgacgcgc ctatcgaaga ccacgcgctc acccctgtcg tcgctgacca ccagcttcaa
      901 ctggtaaggc tgctccagga gccgtttcac gcccatgttg gtcgggtcga aggtgaacgg
      961 caccaggcgg ctcccgtcgc ccgtagcgtc cccgccggtc ggggccccgt cgatcggggg
     1021 tctcacgccg ccacccgtgt cgccaggaag ggtcgtgtcc ggcagcgtga agtccggttg
     1081 cggaccgctc gagacgccgt tgatggtgag gaacaccggg gtgcccggaa ccgtgaagcc
     1141 cgttgggcgc acgtccacca ccccctccgg ccggtcgggg tcctgcacga acgtgatctg
     1201 gccgatccgc aactgcgccg ccagaacggc cagctgcgcc tgccactgcg gctggcccat
     1261 gacgtccggt acctggacga cgttggccgt cgagagggag taggagagca gcacgggggt
     1321 gccgggcgga acgacctcgc ctgcggcggg gctcatgctc acgacggcgc gcggctgcga
     1381 gaaggacac
//
LOCUS       DAOQYE010000714         1387 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_120864_length_1387_cov_2.461712, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000714 DAOQYE010000000
VERSION     DAOQYE010000714.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1387
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120864_length_1387_cov_2.461712"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="PKN52_10440"
     CDS             <1..273
                     /locus_tag="PKN52_10440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00412.1"
                     /translation="RFAAALANIPPRRDALALPAFADTITPHMQTYIDMLLTGYVYTP
                     PGLPVGLYLSDQLNQALAAVQDGTVSAREALETAQRNVQRELEKYR"
     gene            370..1251
                     /locus_tag="PKN52_10445"
     CDS             370..1251
                     /locus_tag="PKN52_10445"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNR00413.1"
                     /translation="MTRRQRRDLWLGLAFVSPWLVGFLIFTVYPVLASLYFSFTDYNV
                     VSAPRWIGMRNYTDLINDPLFGKTLYNTVYLAAIGIPFSLGLSLVIALLLNNKVRFQG
                     LFRTVYFLPSVVPAVAAALLWRWFLNPDYGPVNEFLWQLGVNGPGWLADPGWSKPALI
                     LASLWGVGGSMVIYLAGLQNVPVALYEAADLDGASRWQRFRFVTLPMLSPVILFNLIM
                     GIITSFQAFTNIYIMTGGGPSNSTLVYALYLYQNAFQFFRMGYASAMAWVLLAITAVA
                     LVGVFRTSGWVYYEARS"
BASE COUNT          188 a          534 c          408 g          257 t
ORIGIN      
        1 cgcttcgcgg ccgccctcgc caacatcccg ccgcgccgcg acgccctcgc cctgccggcc
       61 ttcgccgaca ccatcacgcc ccacatgcag acgtacatcg acatgctgct gaccggctac
      121 gtctacacgc cgcccggcct ccccgtcggc ctgtacctga gcgaccaact caaccaggct
      181 ctcgccgccg tccaggacgg cacggtgtcg gcccgcgagg cgctcgagac ggcccagcgc
      241 aacgtgcaac gcgaactgga gaagtaccgc tgatgaaccg ctccccagtc ggtggcggcg
      301 gcgcgcgccg ggtggccccg cacacgcagg agccggcgcc gcgccggacg accgagcgtg
      361 cttaggtcca tgacccgtcg gcagcgccgc gacctctggc tggggttggc cttcgtgtcg
      421 ccctggctgg tcgggttcct gatcttcacc gtctacccgg tgttggcctc gctctacttc
      481 tcgttcaccg actacaacgt ggtgtccgcc ccccgctgga tcggcatgcg caactacacc
      541 gacctgatca acgacccgct cttcgggaag acgctctaca acaccgtcta cctggccgcc
      601 atcggtatcc cgttctcgct cggccttagc ctcgtcatcg ccctcctcct caacaacaag
      661 gtgcgcttcc agggactctt tcgaaccgtc tacttcctgc cgagcgtagt gcccgccgtg
      721 gcggccgccc tcctgtggcg ctggttcctc aacccggact acgggccagt caacgagttc
      781 ctctggcagc tcggcgtcaa cggcccgggc tggctggcgg accccggctg gtccaagccg
      841 gcgctgatcc tcgcgagcct gtggggcgtc ggcggctcga tggtcatcta cctcgccggg
      901 ctgcagaacg tgccggtcgc cctctacgag gcggcggacc tggacggcgc cagccgctgg
      961 cagcgcttcc gcttcgtgac gctcccgatg ctgtcccccg tcatcctctt caacctgatc
     1021 atgggcatca tcacgtcgtt ccaggccttc acgaacatct acatcatgac gggcggcggc
     1081 ccgtccaact ccacgctcgt ctacgccctc tacctgtacc agaacgcctt ccagttcttc
     1141 cggatgggct acgcctcggc catggcgtgg gtgctgctgg cgatcacggc ggtcgcgctc
     1201 gtcggcgtgt tccgcacgtc cggctgggtc tactacgagg cgcgcagctg atggcccggc
     1261 gccgcggcct ctggaacctc gtcgtctacg tcgtgctggt agccggcgcc gtcttcttcc
     1321 tcacgccgtt cgtgtggatg atctcgacct cgctgaagac ggacggtcag gtcttcgcca
     1381 tcccgcc
//
LOCUS       DAOQYE010000715         1386 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_121010_length_1386_cov_2.484598, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000715 DAOQYE010000000
VERSION     DAOQYE010000715.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1386
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121010_length_1386_cov_2.484598"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="PKN52_10450"
     CDS             complement(<1..252)
                     /locus_tag="PKN52_10450"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNR00414.1"
                     /translation="MSGYLECHKLSKAYDGTRALQDVSLRVGRGRVHAVIGENGAGKS
                     TLVNAIAGLVPFDSGTLTLAGRPYRPASPRQAAAAGVGMV"
     gene            complement(347..>1386)
                     /locus_tag="PKN52_10455"
     CDS             complement(347..>1386)
                     /locus_tag="PKN52_10455"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HNR00415.1"
                     /translation="CPMRRSLKTVVALLVSLVMGFGAAQRDAFDVAVFFPGRLGGNPI
                     EPPITAGVERVRAAFPNVTVRVLEGGAASEWESGAMALAASGRYDLIVTFTEGMPQIV
                     QAVSQYFPDQKWAVLDAAAPDQPNAYSLVYSDEALGFLGGAVAGLLAATDGLSRNPGA
                     PTVGLLAGTPYPAMDTKIHPGFAAGAKYVTDDVVVLYAVVGDWNDPNRARDLALGMYG
                     RGATAILAVTGGGDSGVYRAASEMGGYAVGVNTNQNDDVPGVILASVLKRLDNSIYSV
                     VERALVGELPYGTVESVGVEEGAISLADDEYYERYVPEAVRGRLAEIEGGMASGAIDY
                     VGLTAEALEAR"
BASE COUNT          199 a          517 c          467 g          203 t
ORIGIN      
        1 caccatgcct acgcccgcgg ccgcggcctg ccgcggcgag gccgggcggt agggtcggcc
       61 ggcgagcgtc agcgtgccgc tgtcgaacgg gaccaaccca gcgatggcgt tgaccagggt
      121 gctcttgccg gcgccgttct caccgatgac ggcgtggact cggccgcgac cgacacgcaa
      181 gctgacgtcc tgcagggcac gggtcccgtc gtacgccttg ctcagcttgt ggcactcgag
      241 gtagccgctc acgtcacgcc gccctttccc tatcgccccc ccccaacgcg ttcgacgccc
      301 cgcgagcccg ccaagcggag ctcgggcggt cgcgcgttac tcgcctctac cgcgcctcga
      361 gcgcctccgc ggtgagtccg acgtagtcga tggcgcccga cgccatcccc ccctcgatct
      421 cggcgaggcg cccgcggacc gcctccggca cgtaccgctc gtagtactcg tcgtcggcaa
      481 gcgagatggc gccttcctcg accccgacgc tctcgaccgt cccgtacggg agttcgccga
      541 caagggcgcg ttcgaccacg gagtatatgg agttgtcgag gcgcttgagc acgctcgcga
      601 ggatcacgcc tggcacgtcg tcgttctggt tcgtgttgac cccgacggcg tagccaccca
      661 tctcgctggc ggcgcggtac acgccgctgt caccaccgcc cgtgaccgcc aagatcgcgg
      721 tggcgccacg accgtacatg ccgagggcaa ggtcgcgcgc gcggttcggg tcgttccagt
      781 ctccgacgac cgcgtacagg acgaccacgt cgtccgtcac gtacttggcg ccggcggcga
      841 agcccggatg gatcttcgtg tccatggccg ggtacggcgt tccggcgagc aggccgaccg
      901 tcggggcgcc cgggttccgg ctcagcccgt ccgtagcggc cagtaggccc gcgacggccc
      961 cgccgaggaa gccaagcgcc tcgtcggagt acacgagcga gtaggcgttg ggctggtccg
     1021 gcgcggcggc gtcgagcacc gcccacttct ggtccgggaa gtactgggag accgcctgga
     1081 cgatctgcgg catgccctcg gtgaacgtga ctatcaggtc gtacctgccg ctggccgcga
     1141 gcgccatggc gccggactcc cactcggagg cggccccgcc ttcgagcacg cgcaccgtga
     1201 cgttcgggaa cgcggcccgc acgcgctcca caccggccgt gatgggcggc tcgatcgggt
     1261 tgcctcccag gcgacccggg aagaacacgg cgacgtcgaa cgcgtcccgc tgcgccgccc
     1321 cgaaccccat gaccagcgag acgagcagag cgaccacggt cttcagggaa cgtctcatcg
     1381 ggcact
//
LOCUS       DAOQYE010000716         1382 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_121550_length_1382_cov_2.993218, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000716 DAOQYE010000000
VERSION     DAOQYE010000716.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121550_length_1382_cov_2.993218"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="PKN52_10460"
     CDS             complement(<1..446)
                     /locus_tag="PKN52_10460"
                     /inference="COORDINATES: protein motif:HMM:NF013486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HNR00416.1"
                     /translation="MSAAGRKNALDQDLELQGRLAALRERLRSAGADAMLVTNPPNVR
                     YLSAFSSPEDGAVLVTGDAATLYTDARYTTQAGQESLIPVEIVSGIDEAVGDKLTGQR
                     LAVESEHMTLARHRALAKRLGSEPMALDGAFTDLRTPRRWIAAGYSL"
     gene            complement(443..1207)
                     /gene="tpiA"
                     /locus_tag="PKN52_10465"
     CDS             complement(443..1207)
                     /gene="tpiA"
                     /locus_tag="PKN52_10465"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HNR00417.1"
                     /translation="MSRPRQLIAGNWKMNRLPSEATAWTRELLARLDRSPACAGELAL
                     IVPFTHLSALTQLTFGSGVAIGAQDVSAHESGAYTGEVAAAMLADLGVRYVVVGHSER
                     RSYHGESDAVVNAKTRRALEHGLVPIVCVGEKQAERDAGDAERVVLGQVRDGLEGIAV
                     GAPESLVVAYEPVWAIGTGRTATAADAAAMGTALRSELKRLYPTTGDGMRILYGGSMK
                     PGNAAELLAQQDVDGGLIGGASLELDDYLKIAEASR"
BASE COUNT          196 a          468 c          504 g          214 t
ORIGIN      
        1 aggctgtagc ctgcggcaat ccagcgccgg ggcgtgcgca ggtcggtgaa ggcgccgtcc
       61 aaggccatag gctcgctgcc gagccgcttg gcgagggcgc ggtggcgcgc caacgtcatg
      121 tgttcgctct cgaccgccaa acgttggccg gtgagcttgt cacccacggc ctcgtcgatg
      181 cctgacacga tctcgacggg gatgagcgac tcctgaccgg cctgcgtcgt gtagcgggcg
      241 tccgtgtaga gcgtcgccgc gtcgcccgtc accagcacgg cgccgtcctc aggcgaggag
      301 aacgcggaca ggtagcgcac gttgggcggg ttcgtcacca gcatggcgtc cgctccggcc
      361 gagcgcagcc gctcgcgcag cgctgccaag cgcccttgca gctcgagatc ctggtctagc
      421 gcgttcttcc tgcccgcagc gctcatctgg aggcctccgc gatcttcagg tagtcgtcga
      481 gttccaggct ggccccgccg atgagcccac catccacgtc ctgctgagcc aggagctcgg
      541 ccgcgttgcc cggtttcatg ctgcccccgt agaggatgcg catgccgtct cccgttgtgg
      601 ggtagaggcg cttgagttcg gagcggaggg cggttcccat ggcggccgcg tcggcggcgg
      661 tggcggtacg accggtgccg atggcccaca ccggttcgta cgcgaccacc aggctttcgg
      721 gagcgccgac ggcgatgccc tccagcccgt ccctgacctg gccgagcacg acgcgttcgg
      781 cgtcgccggc gtcgcgctcc gcctgcttct cgcccacgca gacgatgggt acgagcccgt
      841 gttcgagggc tcggcgggtc ttcgcgttca cgacggcgtc cgactcaccg tggtacgagc
      901 gtcgctcgga gtggccgacc actacgtaac gcacgccgag gtcggccagc atggcggccg
      961 cgacctcacc cgtgtaggcg ccggactcgt gcgcgctcac gtcctgggcg ccgatagcta
     1021 cgccggaccc gaaggtgagc tgggtgagcg cgctcaggtg cgtgaagggg acgatcaggg
     1081 ccaactcgcc ggcgcaagcc ggcgagcggt cgagccgagc caacagctcg cgcgtccaag
     1141 ccgttgcctc ggacggaagc ctgttcatct tccagttccc tgcgatgagc tgcctagggc
     1201 gggacatgca gccaaggata ccgtagggca catccaggat gccgcggggc ggccgggcgc
     1261 gacggcagcg ccgccgcgca tgggaggggt cgcgaagacg ccgcgccagg ggtcatcgag
     1321 ggtcatgaac gggtccggcg cccggttcag gggcagcgga gtcggtcagg gggatggtcg
     1381 gg
//
LOCUS       DAOQYE010000717         1377 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_122270_length_1377_cov_2.882753, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000717 DAOQYE010000000
VERSION     DAOQYE010000717.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1377
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122270_length_1377_cov_2.882753"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            29..>1377
                     /locus_tag="PKN52_10470"
     CDS             29..>1377
                     /locus_tag="PKN52_10470"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNR00418.1"
                     /translation="MLLWAAYLVGYGEVVGFAGSYAPLAALLPVAVSGWCWGPVGGLA
                     SGVIGYLAVQLRMDALGVATWNPLSLLQADQMFAFLACSGAGLVVGWMRRLEHNLHHE
                     RAARRKALVDPLTGTLVRQAFEERLRAELDAAAAAGGGLALLFVDLDRFKFVNDTYGH
                     DFGDKLLGEVGQVLLANVREEDLVGRVGGDEFMVALVGVRDEQAAGQIARSLVRELST
                     PFTVEGRELQVSASIGVALYPRDDNVVEALLQSADAAMYQVKVAGKNSFNFSTVEVRT
                     RLTRRLELERVLRRALTENEFKVMYQPQVRIADGTLVGFEALLRWHSPDLGLVPPAEF
                     IPVAEEAGMIAPIGHWMLRETAMQLRTWLRQGLMPVRVAVNVSTLQFHQSNFVDTVKG
                     ALDDSGIQPHLFELEVTESVLEQDNDLAVRILKQLQRLGVRIALDDFGTGYSSLAYL"
BASE COUNT          196 a          469 c          474 g          238 t
ORIGIN      
        1 gccgagttgg ctaagcagac tatccccgtt gctcctgtgg gccgcctacc tggtcgggta
       61 cggcgaggtc gtggggttcg ccggctcgta cgcgccactc gcggcgctgc tccctgtggc
      121 ggtcagcggc tggtgttggg ggcccgtcgg cgggctggcc agcggcgtga tcggctacct
      181 ggcggtgcag ctccgcatgg acgccctcgg cgtcgcgacc tggaacccct tgagcctcct
      241 gcaggccgac cagatgttcg ctttcctcgc ctgctccggc gccggcctcg tggtcggttg
      301 gatgcggcgg ctggagcaca acctgcacca cgagcgggcg gcgcgccgca aggcgctcgt
      361 cgacccgctt acgggcacgc tggtgcgcca ggccttcgag gagcgcttgc gggccgagct
      421 cgatgccgcg gccgctgccg gcggcggact tgccctcctc ttcgtcgatc tcgaccgctt
      481 caagttcgtc aacgacacgt acggtcacga cttcggcgac aagcttctcg gcgaggtcgg
      541 ccaggtcctg ctcgccaacg tgcgcgagga ggacctcgtc ggcagggtgg gaggcgacga
      601 gttcatggtc gctctcgtcg gcgtgcgtga tgagcaggcg gccggccaga tcgcgcgcag
      661 cctggtcagg gagctgagta ccccgttcac ggtcgagggt cgcgagctcc aggtgtcggc
      721 gtccatcggc gtggccctct atccgcgcga cgataacgtc gtggaggcgc tcctgcagtc
      781 cgctgacgcc gccatgtacc aggtgaaggt cgccggcaag aactcgttca acttcagcac
      841 ggtggaagtc cggacgcgcc tgacgaggcg gctcgaactc gagcgcgtgc tcaggcgcgc
      901 cctgacggag aacgagttca aggtcatgta ccagccgcag gttcgcatcg ccgacggcac
      961 cctcgtcggc ttcgaggcgc tcttgcgctg gcactccccg gacctaggct tggtgccgcc
     1021 ggccgagttc atcccggtgg ccgaggaggc cggcatgatc gcgcccatcg gccactggat
     1081 gttgcgcgag accgccatgc aactgcgcac atggctgcgc cagggcctga tgcccgtgcg
     1141 cgtcgccgtc aacgtgtcga ccctccagtt ccatcagtcg aacttcgtgg ataccgtcaa
     1201 gggcgccctc gacgactcag ggatccagcc gcacctgttc gagctggaag tcaccgagag
     1261 cgtcctcgag caggacaacg acctggccgt gcggatcctc aagcagctcc aacggctggg
     1321 cgtgcgcata gcgctggacg acttcggcac gggctactcg tcgctcgcct acttgca
//
LOCUS       DAOQYE010000718         1376 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_122386_length_1376_cov_3.247540, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000718 DAOQYE010000000
VERSION     DAOQYE010000718.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1376
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122386_length_1376_cov_3.247540"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1074)
                     /locus_tag="PKN52_10475"
     CDS             complement(<1..1074)
                     /locus_tag="PKN52_10475"
                     /inference="COORDINATES: protein motif:HMM:NF018449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HNR00419.1"
                     /translation="MQGLGRSKLSAGVWLTCLFLAACQSNGPGVQVTIVPRQVVLAPG
                     VRQTFTATVTGTPNTAVSWSTTGGTLENGGSQAMYLAPAVPGDYEVTATSVADPSRLA
                     TAEVRVAAPGETLWTRLMGAQTHDGATCVTIDRDGNIVLAGIVNEGALEGTSAGNADA
                     YVQKRSADGAVLWTRQFGSAEDDAAAGCAVDTDGNVVVVGVTLGSLHGASAGGVDSFV
                     RKYDQDGTMLWARQFGSSGTDEARGVAVDRHGNVVVVGRTDGDFGGPVPDFPDAFIRK
                     YSPIGDILWTQRFNRDQPFDAAAVRTYRGEDEEDLYLVASTAGSNTSTEAFFMTFGSE
                     GNLRATKRFGPEQNSTALAIVNGP"
BASE COUNT          198 a          482 c          445 g          251 t
ORIGIN      
        1 cgggccgttg acgatggcca gggcggtgga gttctgttcc ggcccgaagc gcttggtagc
       61 cctcaggttt ccctccgagc cgaaggtcat gaagaacgcc tccgtgctgg tgttagaacc
      121 cgccgtgctc gcgaccaagt acaagtcctc ctcgtcctca ccccggtaag tccggaccgc
      181 cgcggcatcg aacggttgat ctctgttgaa ccgttgggtc cagaggatgt cgccgatcgg
      241 gctgtacttc ctgatgaacg catctgggaa gtctggcacc ggcccgccga agtcgccgtc
      301 cgttcggcca acgaccacca cgttgccgtg gcggtcgacg gccacgcctc gcgcctcgtc
      361 ggtcccgctc gagccgaact gccgcgccca cagcatggtg ccgtcctggt cgtacttcct
      421 cacgaaggag tccacgccac ccgcgctcgc accgtgcagc gaaccgagcg ttacccccac
      481 cacgacaacg ttgccatccg tgtctacggc acagccggcc gcggcgtcgt cctccgccga
      541 gccgaactgc cgcgtccaga gcacggcgcc gtcggcgctg cgcttctgca cgtaggcgtc
      601 tgcgttgccc gcgctagtac cctcaagggc tccctcgttg acgatgcccg ccagcacgat
      661 gttcccatcg cgatcgatcg tcacacacgt ggcgccgtcg tgcgtttgcg cgcccatcag
      721 cctcgtccag agcgtttcac ctggcgccgc gaccctgacc tcggcggtgg ccaacctcga
      781 tgggtctgcc acgctcgttg ccgtgacctc gtagtcgccc ggaacggccg gggctaggta
      841 catggcttgg ctgcccccgt tctctaaggt gccgcctgtc gtgctccaac tcacggccgt
      901 gttcggcgtg ccggtaacgg tggccgtgaa cgtctgcctt acgcctgggg cgagaacgac
      961 ttgccttggc acgatggtca cttgaacgcc tgggccgttg ctctggcagg ccgcgaggaa
     1021 caagcatgtt agccaaacac ctgccgagag cttgctgcgc ccgagtcctt gcatggacgt
     1081 acctccggtg gccctgcccg cggtgccctt gtgcgagtag tcgaggtccg tgcggcggtg
     1141 ctatgtctcc gggcctgtcg aggaggttgg cgagcctggc ggattctatg cgccccgcga
     1201 acgggctggc cctgcacggc gcacagcggg cgcctcagct gcaaggccgg gttcgtgtgg
     1261 cgcgtagcct gtaccggggt atcagacacg cccatgcaag gtgcagcgcc atccggtggg
     1321 taacagtgac gctgcccggc accgcgccac ggcaccgtgt ggggcgggtc agggcg
//
LOCUS       DAOQYE010000719         1375 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_122615_length_1375_cov_2.074242, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000719 DAOQYE010000000
VERSION     DAOQYE010000719.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1375
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122615_length_1375_cov_2.074242"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..964)
                     /locus_tag="PKN52_10480"
     CDS             complement(<1..964)
                     /locus_tag="PKN52_10480"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNR00420.1"
                     /translation="MILLKDCFRVVRPRYGAGPAAGSVGDDLSGVDVLIDGNRIAKIG
                     KALPVPAGACKVVDASRHVVMPGLVNTHHHFYQTLTRNVPAVQDAKLFDWLVYLYEVW
                     KGIDEEAVYWSSMLAMAELAKTGCALSTDHHYLYPRSFTGDLPGIQFKAAADIGLRFA
                     PTRGSMSRSKKDGGLPPDSVVQDEDEILAQSEEALRKYHDAAPDAMRKVALAPCSPFS
                     VSEKSMRDAADLARRYGARLHTHLAETNDEDDFCVQMYGRRPLKVMEDCGFVGKDVWY
                     AHGIHFNDDELDYLARTGTGVAHCPSSNMRLGSGICRVREMLDRG"
     gene            complement(961..>1375)
                     /locus_tag="PKN52_10485"
     CDS             complement(961..>1375)
                     /locus_tag="PKN52_10485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00421.1"
                     /translation="LLGISGIGNLVATIKAAKFYELRDDDIALTVFTDSLQLYGSRLR
                     ELTAERGPYTQRQADKDLDLLQNLSVDHVLDMGHIERRRVHQLKYYTWIEQMGKSLDE
                     LNEQWDNYRQYWGKLHDQVDTIDQMIKDFNAEVLK"
BASE COUNT          208 a          424 c          480 g          263 t
ORIGIN      
        1 cgccgcggtc gagcatctcg cgcacgcggc agatgccgga gccgagcctc atgttcgacg
       61 acgggcagtg agcgacgccg gtgcccgtcc tggccaggta gtcgagctcg tcgtcgttga
      121 agtgtatgcc gtgggcgtac cagacgtcct tcccgacgaa gccgcagtcc tccatcacct
      181 tgagcgggcg gcggccgtac atctgcacgc agaagtcgtc ctcgtcgttc gtctcggcca
      241 ggtgggtatg gaggcgcgct ccgtacctgc gcgccaggtc ggccgcgtcg cgcatcgact
      301 tctcggagac cgagaacggc gagcagggcg cgagcgctac cttgcgcatc gcgtcgggcg
      361 ccgcgtcgtg gtacttcctg agcgcttcct cgctctgggc gaggatctcg tcctcgtcct
      421 gcacgacgct gtccggcggg aggccgccgt ccttcttgga gcgcgacatc gagccgcggg
      481 tcggcgcgaa gcgcaggcct atgtcggcgg ccgccttgaa ctggatgccg ggcaggtccc
      541 cggtgaacga gcgcgggtac aggtagtggt ggtcggtcga gagggcgcat ccggtcttgg
      601 ctagctccgc catcgcgagc atgctcgacc agtagacggc ttcctcgtcg atgcccttcc
      661 agacctcgta gaggtagacg agccagtcga acagcttggc gtcctggacg gccggcacgt
      721 tgcgcgtcag cgtctggtag aagtggtggt gcgtgttgac gagtccgggc atgacgacgt
      781 ggcggctcgc gtcgacgacc ttgcaggcgc cggccgggac cgggagcgcc ttgccgatct
      841 tggcgatgcg attgccgtct atgagcacgt ctacgccgga caggtcatcc ccgacggaac
      901 cggccgcggg gccggcgccg tagcgggggc ggaccaccct gaagcagtcc ttgagcagta
      961 tcacttgaga acctccgcgt tgaagtcctt gatcatctgg tcgatggtgt cgacctggtc
     1021 atggagtttg ccccagtact ggcggtaatt gtcccactgc tcgttgagct cgtccaggct
     1081 tttgcccatc tgctcgatcc aggtgtagta cttgagctgg tggacgcggc ggcgctcaat
     1141 atggcccatg tccagcacgt ggtccacgga caggttctgg agcaggtcaa ggtccttgtc
     1201 ggcctggcgc tgggtgtagg ggccgcgctc ggcggtcagc tccctgagcc gcgatccgta
     1261 cagctggagg gaatcggtga acacggtcag ggcaatgtcg tcgtccctga gctcgtagaa
     1321 cttggcagcc ttgatggttg ccacaaggtt gccaatgccg gatataccca ggagg
//
LOCUS       DAOQYE010000720         1371 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_123130_length_1371_cov_3.927052, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000720 DAOQYE010000000
VERSION     DAOQYE010000720.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1371
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123130_length_1371_cov_3.927052"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /locus_tag="PKN52_10490"
     CDS             <1..536
                     /locus_tag="PKN52_10490"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNR00422.1"
                     /translation="TRVVSWLLGGSLAVMAWLAAFFFGVTQQPDPAVEAIINARPVAP
                     ATASADGQKVFDQICASCHMPTGAGLPGAFPPLAGHLPELLAAGGRDYVKQVVLYGLQ
                     GAITVGGQTYNSVMPPWQQLSDQEIADALNYAATAWGNALPAGESSFEASEIAAARGQ
                     GLNAQQVHDARERVGLP"
     gene            complement(707..>1371)
                     /locus_tag="PKN52_10495"
     CDS             complement(707..>1371)
                     /locus_tag="PKN52_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNR00423.1"
                     /translation="CRELIAGGAIGTVVGAVAFMANHGMEHWHANPEFFYQPGAGPLF
                     DIGPYYLTALVNLLGPVSRVAGAVNTGLSERPITTGPASGRTIKVGTPTHVSALLTFA
                     SGAVVPMLYSFDVWASTLPRIEVYGTGGTLGVPDPNTFGGPVKIRRAGEDAWSEAPLL
                     PGSQENSRGIGLEDMVEAMRRGAPHRASGELALHVLEVMESVLVAARESRVVTLRTSA
                     PA"
BASE COUNT          209 a          463 c          478 g          221 t
ORIGIN      
        1 acacccgagt ggtctcgtgg ctgcttggcg gttcgctcgc ggtcatggcc tggttggccg
       61 cgttcttctt cggggtcacg cagcagcccg acccggccgt cgaggccatc atcaacgcgc
      121 gccccgtggc gcctgcgaca gcctcggccg acggccagaa ggtcttcgac cagatctgcg
      181 cttcctgcca catgccgacg ggcgccggtc tgcccggagc gttcccgccg ttggccgggc
      241 acctcccgga actactggcg gcgggtgggc gtgactacgt gaagcaagtc gttctctacg
      301 gtctgcaggg tgccatcacc gtcggtgggc agacgtacaa cagcgtcatg ccgccgtggc
      361 agcagctgag cgatcaggag atcgccgatg cgctcaacta cgccgcgacg gcctggggga
      421 acgcgctgcc agcgggagaa tcgtccttcg aagccagtga gatcgcggcc gcgcggggtc
      481 agggactgaa tgcccagcag gtccatgacg cgcgggagcg ggtggggctg ccttgatcgt
      541 cgtgcccggc gactatcctc gatggtcgcc gggccaccgt ctagggtcaa cgggagacca
      601 cgtggctcgc ggatcttgag tcgacatacc gtgccattcg acgaggccct gtcttctcag
      661 cgcctcgagg cggccgagat gtcgtgaata ccgagcacgg cccaggctac gccggggcgc
      721 tggtcctcaa cgtcaccacc cgcgactcgc gcgccgcgac gagcacggac tccatcactt
      781 ccagtacgtg cagagcgagt tcgccgctgg cccggtgggg cgcgccgcgg cgcatggcct
      841 caaccatgtc ctcgaggccg atgccgcggc tgttctcctg cgagccgggt agtagcggcg
      901 cctcgctcca ggcatcctcc cctgccctcc ggatcttgac cgggccgccg aaggtgttcg
      961 ggtccggcac gccgagcgtg cctcccgtgc cgtatacctc gatacgcggc aacgtggagg
     1021 cccagacgtc gaagctgtac agcatgggga ccacggcgcc ggatgcgaag gtgaggagcg
     1081 cggagacgtg cgtcggggtg ccgaccttga tggtcctgcc gctcgcgggt ccggtggtga
     1141 tgggccgctc tgacaggccc gtgttgaccg cgccggccac ccggctcacc gggccaagca
     1201 ggttcacgag ggccgtgagg tagtagggcc cgatatcgaa gaggggtccg gcacccggct
     1261 ggtagaagaa ctccgggttg gcatgccaat gctccatccc gtggttagcc atgaaggcaa
     1321 cggccccgac taccgtgccg atggcgccgc ccgcgatgag ctctcgacac g
//
LOCUS       DAOQYE010000721         1368 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_123577_length_1368_cov_3.769992, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000721 DAOQYE010000000
VERSION     DAOQYE010000721.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123577_length_1368_cov_3.769992"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..796)
                     /locus_tag="PKN52_10500"
     CDS             complement(<1..796)
                     /locus_tag="PKN52_10500"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNR00424.1"
                     /translation="MGGAPVGNSSVRVWLLQRSDVLGGTKAIIDGLERRLVAQGVDAS
                     AVYLRDLREHEAARESRWAGPLLNVRDLGRLWLRLTRERPAVIVTFTPLLGVVAALAA
                     RLATRSRVVSTLHMTSDRIGKGVLKLDAAAARRGWYSRIVACAPSVAASYAGNDAAYT
                     RHMTVIPNGVEPSAEPRRGRSAELRTEVQVPPEGRVAVAVGRLTADKNYGVLFEALAA
                     APGWWLILLGGGEARAELEARARDHGVVERVRFLGRVPRERVGGWLS"
     gene            complement(804..>1368)
                     /locus_tag="PKN52_10505"
     CDS             complement(804..>1368)
                     /locus_tag="PKN52_10505"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNR00425.1"
                     /translation="FVALVLVCVTVVGTVMTIAPDSLFAAVGRDASVTGRTTLWSLVL
                     DKVAEQPVLGHGYRAFWGSPGGEAISRTLAWRVTHAHNSWLDVGLDLGAVGSLLVAAL
                     LLLPLKHWLGARRSAMRYHETGVVVVVSTLLVSISDSVLLGPNNLFLLLLFVQCMAAV
                     KAHAPVATSEPSGAGTAVGRAAFVERA"
BASE COUNT          224 a          510 c          454 g          180 t
ORIGIN      
        1 ccgagagcca cccgccgacg cgttcgcgcg gcacgcgtcc gaggaaccgt acccgttcca
       61 cgaccccgtg gtcgcgggca cgagcctcca actcagcccg ggcctcgcct ccaccgagga
      121 gtatcagcca ccagcccggc gcggctgcaa gcgcctcgaa cagcacgccg tagttcttgt
      181 ccgcggtgag gcggccgacg gcgaccgcca ctcgcccctc cggtgggacc tggacctcgg
      241 tgcgcagttc cgccgacctg cctcgcctcg gctcggcgct cggctccacg ccgttgggga
      301 tgaccgtcat atgccgcgtg taggccgcgt cgttgcccgc gtagctcgcc gcgacggacg
      361 gggcgcatgc aacgatcctg ctgtaccagc cgcgtcgcgc ggccgccgcg tccagcttga
      421 gcaccccctt gccgatgcgg tcgctggtca tgtgcagggt cgagacgacc ctcgaccggg
      481 tcgccagcct ggccgcgagc gcagccacga ccccgaggag gggcgtgaac gtgacgatca
      541 cggcggggcg ttctcgcgtc aagcgcagcc acagcctgcc gaggtccctg acgttcagga
      601 ggggaccggc ccagcggctc tcacgagccg cctcgtgctc ccgcaggtcg cgcaggtaca
      661 cggcggaagc gtcgaccccc tgggcgacca gcctgcgctc gagcccgtcg attatcgcct
      721 tggtacctcc gaggacgtcg ctcctctgca gcagccatac tcggaccgac gagttcccga
      781 ccggcgcgcc gcccacggct ccgctaggcc ctctcgacga aggcggcgcg accaaccgca
      841 gtgcccgcgc ccgacggctc gctcgtggct acgggcgcgt gcgccttgac agcggccatg
      901 cactggacga agaggaggag caggaagagg ttgttcggac ccaagagcac cgagtcggag
      961 atggagacga ggagcgttga caccacgacg acgacacctg tctcgtggta cctcatggcc
     1021 gagcgcctgg ccccgagcca gtgcttgagg ggcagtagta gcaacgcggc gacgagcagg
     1081 ctgcctaccg ccccaaggtc gagccccacg tcaagccaac tattgtgcgc gtgggtcacg
     1141 cgccaggcca acgtccggct gatggcctcg ccacccggac taccccagaa cgcccggtag
     1201 ccgtgaccga gaacgggctg ctccgcgacc ttgtcgagga ccagcgacca caaggtggtc
     1261 cgccccgtca cggaggcgtc tcggcccacc gcggcgaaca ggctgtccgg cgcgatggtc
     1321 atcaccgtgc cgaccaccgt aacgcacacg agcacgaggg ctacgaag
//
LOCUS       DAOQYE010000722         1368 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_123612_length_1368_cov_2.666413, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000722 DAOQYE010000000
VERSION     DAOQYE010000722.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123612_length_1368_cov_2.666413"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1057
                     /locus_tag="PKN52_10510"
     CDS             <1..1057
                     /locus_tag="PKN52_10510"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HNR00426.1"
                     /translation="AATLVGSAVAAHMARSLTSRLSLLFAGSLGGLVAAVALVGLVLL
                     GGGMTPGGMAGGAGLLFAGGVLAGVLALGLLPIAESGFGFLTDFRLVELSSPNHPLLQ
                     RLITEAPGTYQHSLIISNLVDQAVKNVGGNALLARVGALYHDVGKVKRPGFFVENQFS
                     GDNPHDRVSPHLSYLIITSHVRDGVELLQEYHMPEELLDFAAEHHGTTVLSYFYKRAL
                     DEGQVEELNFRYPGPKPRSKETGILMLADAVESASRTLSDPTPTSIRSLIDRLVKQRL
                     QDGQLDETPLNFHDLEVVSDTFQRMLTAILHRRINYPSAEEVRNLKRTKSEAPTKEGG
                     QGGARRGVGRDGTLPSR"
     gene            1071..>1368
                     /gene="ybeY"
                     /locus_tag="PKN52_10515"
     CDS             1071..>1368
                     /gene="ybeY"
                     /locus_tag="PKN52_10515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HNR00427.1"
                     /translation="MLVAYLEAEGWGERELTVVLLDDAAMAERNEHDRGVAGATDVLS
                     YPTFEPDGAWFPGVAHLGDVFISVDTARRQAAEHGHDLETEVLVLAAHAITHLAG"
BASE COUNT          219 a          502 c          443 g          204 t
ORIGIN      
        1 cgccgcgacc ctcgtcggca gcgccgtcgc cgcccacatg gcgcggagcc tcacgagccg
       61 cctcagcctc ctcttcgcag ggagcctcgg cggtctcgtc gcggccgtgg cgctcgtcgg
      121 cctcgtgcta ctcggcggtg gcatgacgcc gggtggcatg gccggcgggg cgggcctgct
      181 gttcgcgggc ggcgttctcg ccggcgtgtt ggcgctcggc ctcctcccga tcgccgagag
      241 cgggttcggc ttcttgacgg acttccggct ggtcgagctg tcgagcccca accacccgct
      301 cctccagcgc ctgataaccg aggcgcccgg cacctaccag cacagcctca tcatctcgaa
      361 cctcgtcgac caggccgtca agaacgtggg cggcaacgcc ctgctcgcca gggtcggcgc
      421 cctttaccat gacgtcggca aggtaaagcg tccggggttc ttcgtcgaga accagttctc
      481 cggcgacaac ccgcacgacc gcgtcagccc gcacctgtcc tacctgatca tcacgagcca
      541 cgtgcgcgac ggcgtcgagc tgctgcagga gtaccacatg cccgaggagc tcctcgactt
      601 cgcggccgag caccacggca cgacggtgct cagctacttc tacaagcgcg ccctcgacga
      661 gggccaggtc gaagagctca acttccgcta ccccggcccg aagccgcgct ccaaggagac
      721 gggcatcctc atgctcgccg acgcggtcga gtcggcctcg cggaccttgt cggatccgac
      781 cccgacgagc atccgctcgc tcatcgaccg cctcgtcaag cagcgcctgc aggacgggca
      841 gctggacgag acgcccctca acttccacga cctcgaggtc gtgtcagaca ccttccagcg
      901 catgttgacg gccatcctgc accgccgcat caactacccg agcgccgagg aggtacgcaa
      961 cctcaaacgg accaagtccg aggcaccgac caaggagggc ggccagggtg gcgcacgtag
     1021 aggtgttgga cgagacggca cgcttccctc gcgttgaacg tctcgaaacg gtgctcgtcg
     1081 cctacctgga ggcggaaggc tggggcgagc gcgagctgac cgtcgtcctg ctggacgacg
     1141 cggccatggc ggagcgtaac gagcatgaca ggggcgttgc cggcgcaacg gacgtgctct
     1201 cctaccccac gttcgagccg gacggagcgt ggttcccggg cgtggcgcac ctcggcgacg
     1261 tcttcatcag cgtcgacacc gcgcggcgcc aggcggcgga gcacggtcac gacctcgaga
     1321 cggaggtgct cgttctcgcg gcgcacgcca tcacgcacct cgccgggt
//
LOCUS       DAOQYE010000723         1367 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_123720_length_1367_cov_3.576220, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000723 DAOQYE010000000
VERSION     DAOQYE010000723.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1367
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123720_length_1367_cov_3.576220"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            85..1062
                     /gene="floA"
                     /locus_tag="PKN52_10520"
     CDS             85..1062
                     /gene="floA"
                     /locus_tag="PKN52_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176964.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HNR00428.1"
                     /translation="MDIPLLILAGAVILFFIILFNFIPVGLWISAVAAGVKVSLFSLV
                     AMRLRRVPPNQIILPLIKADKAGIEVNQNQLEAHFLAGGNVNRVVDALIAADKARIAL
                     PFDRAAAIDLAGRDVLDAVKVSVNPRVIQTPNVSAVAKDGIELIATARITVRANLERL
                     VGGAGEETIIARVGEGIVSSIGSTDSHKHTLENPDIISKTVLAKGLDSGTAFEILSID
                     IADVNVGRNIGAQLQTDQAEADKRVAQAKAEERRAMAVAAEQENRAHVEAMRAKVVEA
                     EAEVPQAIAQAFRDGHLGVMDYYSLQNIQSDTTMRGSIGRAAEEGSDRG"
     gene            1059..>1367
                     /locus_tag="PKN52_10525"
     CDS             1059..>1367
                     /locus_tag="PKN52_10525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00429.1"
                     /translation="MNIGFEEIIGLIFFVFFFVIPLFTKKKGPQGGQAPGKPGQPAAG
                     GPGKSAAGPAQASPGPQARGAQASTTPGRQPQSFREMLEEIQRRVAEAEQTARKDAGS
                     T"
BASE COUNT          248 a          494 c          439 g          186 t
ORIGIN      
        1 tccatcgccg cccggggcga cgagtagaac cggaatcacg ctagccagcg ccgccaacga
       61 gcgcagcagc cacaaggaga gcacatggac atcccgctac tgatcctcgc cggcgcagta
      121 atcctcttct tcatcatcct cttcaacttc atccccgtcg gcttatggat ctcggccgtc
      181 gccgcaggcg tcaaggtctc gctcttctcg ctcgtcgcca tgcgcctgcg gcgcgtgccc
      241 cccaaccaga tcatcctgcc gctcatcaag gccgacaagg cgggtatcga ggtcaaccag
      301 aaccagctgg aagcccactt cctggccggt ggcaacgtca accgcgtcgt cgacgcgctc
      361 atcgcggccg acaaggcgcg catcgcgctg ccgttcgacc gtgccgccgc catcgacctg
      421 gccgggcgtg acgtgctcga cgccgtcaag gtctccgtca acccgcgcgt catccagacg
      481 cccaacgtca gcgcggtcgc caaggacggc atcgagctca tcgccaccgc ccgcatcacg
      541 gtgcgcgcga acctagagcg cctcgtaggc ggcgctggcg aggagacgat catcgcccgc
      601 gtcggggagg gcatcgtctc ctccatcggc tcgaccgaca gccacaagca cacgctcgag
      661 aacccggaca tcatctccaa gaccgtgctg gccaagggcc tcgactcggg cacggcgttc
      721 gagatcctct cgatcgacat cgccgacgtc aacgtcggtc gcaacatcgg cgcccaactg
      781 cagaccgacc aggcggaggc ggacaagcgc gtggcgcagg cgaaggccga ggaacgccgc
      841 gccatggccg tggcggccga gcaggagaac cgcgcccacg tcgaggccat gcgcgccaag
      901 gtcgtggagg ccgaggccga ggtgccgcag gccatcgccc aggcgttccg cgacggccat
      961 ctgggcgtca tggactacta ctcgctgcag aacatccaat cggataccac catgcgaggc
     1021 agcatcggac gggccgccga ggagggtagc gaccgagggt gaacatcggc ttcgaggaga
     1081 tcatcgggct gatcttcttc gtcttcttct tcgtcatccc gctgttcacg aagaagaagg
     1141 ggccgcaggg cggtcaggcg cctggcaagc ccggccagcc ggcagccggc ggtcccggga
     1201 agagcgcggc gggcccggct caggcgtccc ccggtccgca ggctcgcggc gcgcaggcca
     1261 gtaccactcc cgggcgccag ccgcagtcct tccgcgagat gcttgaagag atccagcggc
     1321 gggtcgccga ggccgagcag accgcgcgga aggacgccgg gtcgacc
//
LOCUS       DAOQYE010000724         1367 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_123814_length_1367_cov_2.052591, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000724 DAOQYE010000000
VERSION     DAOQYE010000724.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1367
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123814_length_1367_cov_2.052591"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1262
                     /gene="purF"
                     /locus_tag="PKN52_10530"
     CDS             <1..1262
                     /gene="purF"
                     /locus_tag="PKN52_10530"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HNR00430.1"
                     /translation="MGLVAEVFTEERLEKLRFQGANQGVAHTRYSVTGSSLRFNAQPL
                     TARSNKGHLALAHNGNFTNAREIRDVLLAQGVVFQTSNDSEVMLNLIARYSDLGLVDA
                     TARAMTELEGGFAVVIMDQRRVIGLRDAHGVRPLVVGRLDGGFVFASEPAALALVGAE
                     FVRDVAPGELVVADERGITSHQVVSGTPTPCAFEWIYFARGDASLDGVDVHAARVRMG
                     AELAREAPVEADVVVGVPESGLAAAVGYARDSGVPYDIGLYKSPYAGRTFINPTQTLR
                     DLKVKLKLRPTAAVRGKRVVLVDDSIVRGTTSGRIVQLLREAGAAEVHFRVSSPPIKY
                     PCYYGIDTAARKELAAATMSVEEIRRRIGADTLHFLSIDGLARALGLPGVCLACFDGR
                     YPAGRPSALAEAEGLESVGWGRADAAR"
BASE COUNT          193 a          475 c          506 g          193 t
ORIGIN      
        1 acatgggcct ggtcgccgaa gtgttcacgg aggagcgcct ggagaagctg cgtttccagg
       61 gcgcgaacca gggcgtggcc cacacgcgct actctgtgac gggctcgagc ctgcgcttca
      121 acgcgcagcc gctcacggcc cggtccaaca agggccacct cgcgctcgcg cacaacggca
      181 acttcacgaa cgccagggag atccgcgacg tcctgctggc ccaaggcgtc gtgttccaga
      241 cgagcaacga cagcgaggtg atgctcaacc tcatcgcgcg ttacagcgac ctggggttgg
      301 tggacgccac ggcgcgcgcg atgaccgagc tggagggcgg cttcgcggtc gtcatcatgg
      361 accagcggcg cgtgatcggc ctgcgcgacg cccacggggt gcgcccgctc gtggtcggcc
      421 ggctcgacgg cggcttcgtc ttcgcctcgg agccggcggc gctggccctg gtgggcgctg
      481 agttcgtcag ggacgtggct cccggcgagc tcgtggtcgc ggacgagcgg ggtatcacgt
      541 cgcaccaggt cgtgtcgggc acgccgaccc cttgcgcctt cgagtggatc tacttcgccc
      601 gcggcgacgc cagcctcgac ggtgtcgacg tgcacgcggc ccgcgtgcgc atgggcgccg
      661 agctcgcgcg cgaggccccg gtggaggcgg acgtcgtggt gggggtgccg gagtccgggc
      721 tggcggccgc ggtcggttac gcgcgcgaca gcggcgtgcc ttacgacatc gggctctaca
      781 agtcacctta cgcggggcgg acgttcatca accctacgca aacgctccgc gacctaaagg
      841 tgaagctaaa gctccgaccg acggcggcgg tgcgtgggaa gcgcgtagtg ctggtcgacg
      901 actccatcgt ccgcggcacg acgtcggggc gcatcgtgca actcctacgc gaggccgggg
      961 cggcggaggt ccacttccgc gtctccagcc cgccgatcaa gtacccgtgc tactacggca
     1021 tagacaccgc ggcccgcaaa gagctcgctg ctgcaacgat gagcgtggag gagatcaggc
     1081 ggcgcatcgg ggccgacacg ctccacttcc tgagcatcga cggcctggcc agggccctcg
     1141 gcctgccggg cgtctgcctc gcctgtttcg acggccggta tcccgccggg cggccaagcg
     1201 cgctggccga ggccgaaggc cttgagtcgg tggggtgggg gcgcgccgac gcggcgcgct
     1261 gacccgcgac gccccgacgc ggcgccatga cccgcggcgc gctgacccgc gacgcccccg
     1321 cgctcgcgtg ttggagccgg tgcgcgcaag cgcggccggg tggcgcg
//
LOCUS       DAOQYE010000725         1365 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_124033_length_1365_cov_3.164885, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000725 DAOQYE010000000
VERSION     DAOQYE010000725.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1365
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124033_length_1365_cov_3.164885"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..934
                     /locus_tag="PKN52_10535"
     CDS             <1..934
                     /locus_tag="PKN52_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNR00431.1"
                     /translation="QITQQLQELQKAGAGVQRIGELMGMRSALPRDGTTRLPKGPLSV
                     EFDDVSFHYPDVGPDQLTLDGVSFRLAPGTRVGLLGRTGSGKTTLTRLLFRLYDPTGG
                     SVLLSGTDAKDADLDGLRARVGLVSQEVQLFRGTLRHNLSFFDDSVSDERILAVLSEL
                     GMTDWLERQDDGLDTLIDSGGRNLSAGEAQLIAFARVFLADPGLIVLDEPSSRLDPAT
                     ERRLEAAMERLLAGRTAIVIAHRLETVERVDEILVMDRGRLVEHGRRDRLAQDPDSRY
                     ARLRKAALSLDLALAGREGLASHEAHDEALEELA"
     gene            931..>1365
                     /locus_tag="PKN52_10540"
     CDS             931..>1365
                     /locus_tag="PKN52_10540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNR00432.1"
                     /translation="MTFSTNPLKLVMALGRQRRAYFIGTAFLWATIHATPIIFGLAMR
                     GIFDALSGSAGAGANAWSFLAMGLGLDVLRIGLFVGGIYTWATWWLEMTLHVRSSLLR
                     YLVEASGSRRLPDSPSEAVTRFRDDVNDIGEYYEYWCDFWGFA"
BASE COUNT          196 a          487 c          457 g          225 t
ORIGIN      
        1 gcagatcacg cagcagcttc aggagttgca gaaggcgggc gccggcgtgc agcggatcgg
       61 cgagctcatg ggcatgcgct cggccctgcc gcgcgacggc acgacacgct tgcccaaggg
      121 tccgctgtcg gtcgagttcg acgacgtgag cttccactac cccgacgttg gccccgatca
      181 gctcaccctc gacggcgtca gcttccgcct cgcgccgggc acccgggtgg gtctcctcgg
      241 ccgcacgggc tccggcaaga cgaccctcac gcgtctcctc ttccgcctct acgaccctac
      301 gggcggctcg gtgctgctca gcggcaccga tgcgaaggac gccgacctag acggtctgcg
      361 ggctcgggtc ggcctggtct cgcaggaggt ccagctcttt cgcgggacgc tcaggcacaa
      421 cctctcgttc ttcgacgaca gcgtgagcga cgagcgcatc ctggcggtac tgagcgagct
      481 cggcatgacg gactggctcg agcggcagga cgacggcctc gacacgctca tagactccgg
      541 cggccgcaac ctctccgccg gcgaggcgca gctcatcgcc ttcgcccgcg tcttcctggc
      601 cgaccccggg ctcatcgtgc tagacgagcc gtcttcgcgc ctcgaccccg ccaccgagcg
      661 ccgcctcgag gccgcgatgg aacgtctcct ggcaggccgc acggccatcg tgatcgctca
      721 caggctcgag acagtggaac gcgtcgacga gatcctcgtc atggaccgcg gccgcctcgt
      781 ggaacacggt cgccgcgatc ggctggcgca ggatccggac tcgcgttacg cgcggctccg
      841 caaggccgcg ttgagcctgg acctcgccct cgccggacgc gaggggctgg cctcacatga
      901 ggcgcatgac gaagcgctgg aggaactggc atgaccttct cgaccaaccc cctgaagctg
      961 gtgatggcgc tcggacgcca gcggcgggcc tacttcatcg gtaccgcctt cttgtgggcg
     1021 acgatacatg cgacgcccat catcttcggc ctcgccatgc gcggcatctt cgacgcgctc
     1081 tccggctcgg cgggcgcggg cgccaacgcc tggagcttcc tggccatggg gctcgggctc
     1141 gacgtgctcc ggatcggcct gttcgtcggc ggcatctaca cctgggccac gtggtggctg
     1201 gagatgacgc tgcacgtgcg cagttcgctg ttgcgctacc tcgtggaggc gagcggctcg
     1261 cgccggcttc ccgattcgcc gagcgaggcc gtgacgcgct tccgcgacga cgtcaacgac
     1321 atcggcgagt actacgagta ctggtgcgat ttctgggggt tcgcg
//
LOCUS       DAOQYE010000726         1365 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_124066_length_1365_cov_2.568702, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000726 DAOQYE010000000
VERSION     DAOQYE010000726.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1365
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124066_length_1365_cov_2.568702"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..694)
                     /locus_tag="PKN52_10545"
     CDS             complement(<1..694)
                     /locus_tag="PKN52_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNR00433.1"
                     /translation="MADTIESVLQEAREFQPPKAFQRAALLNDATEYERLYRQSLDDP
                     ETFWGEAAKRHHWFEPWTQVREWNEPHVKWFVNGKTNLAYDCLDRQVNEGKANKVAFF
                     WEGEPGDKRTITYGQMLTEVKRFANVLKGKGVRTGDRVAIYMPMIPEAVVAMLACARI
                     GAPHSVVFGGFSSHALSDRINDAQASVVITADGGFRRGAVLPLKPAVDEALASAPSVK
                     HVIAVRRAENTID"
     gene            complement(881..>1365)
                     /locus_tag="PKN52_10550"
     CDS             complement(881..>1365)
                     /locus_tag="PKN52_10550"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HNR00434.1"
                     /translation="PAQAVLLTLMFVGGSAGSASGGVKVLRWLMIAKITGREVRRALH
                     PRAVMPIRIGGRIIPEDALRAVAAFLTLYVGLFAFTAIVLVFLGADFTAAFSTAIASL
                     GNTGPGLNGFAPIGELEHLPALGKLVLMFVMCAGRLEVVTVFVIFTPGWWQLPRRRRN
                     "
BASE COUNT          203 a          457 c          482 g          223 t
ORIGIN      
        1 tgtcgatcgt gttctcggcg cgccgcacgg cgatcacgtg cttcacgctc ggggcggagg
       61 cgagcgcctc gtcgaccgcc ggcttcagcg gcagcacggc gccgcggcgg aagccgccgt
      121 cggccgtgat caccacgctg gcctgcgcgt cgttgatgcg atccgacagg gcgtgcgagg
      181 agaagccgcc gaacacgacg gagtgcgggg cgccgatgcg cgcgcacgcg agcatggcga
      241 cgaccgcctc ggggatcatc ggcatgtaga tggccacacg gtcgcccgtc ctcacgccct
      301 tgcccttgag gacgttggcg aagcgcttca cctcggtgag catctggccg taagtgatgg
      361 tgcgcttgtc gcccggctcg ccctcccaga agaacgcgac cttgttggcc ttgccctcgt
      421 tcacctggcg gtcgaggcag tcgtacgcga ggttcgtctt gccgttcacg aaccacttga
      481 cgtgcggctc gttccactcg cgcacttgcg tccacggctc gaaccagtgg tggcgcttgg
      541 cggcctcgcc ccagaacgtc tccgggtcgt cgagcgattg gcgatagagg cgctcgtact
      601 cggtggcgtc gttcagcagg gcggcgcgct ggaaggcctt gggcggctgg aactcgcgcg
      661 cctcttgcag gacggattcg atggtgtctg ccatgggtcc tcccggatca tggagtctgc
      721 ggcgtggcgt tcttgtgtga gcgtagcgcg cgcctgacag tggtcctgtt cgtcgccgtg
      781 gagcatagcc cagcggcggg tgcgtggcaa acgccgtaac gtaccgtgcg cgggccattg
      841 gcaggctcct cgcgcggcgg cctgcgcctg tgcgtgccgg tcagttccgc cgccgccgcg
      901 gcagctgcca ccaccccggg gtgaagatca cgaacacagt caccacctcg aggcgccccg
      961 cgcacatcac gaacatgagc acgagtttgc cgagggcggg taggtgctcc agttcgccga
     1021 tgggggcgaa gccgttgagc cccggcccag tgttgcccaa cgaagcgatg gccgtgctga
     1081 aggcggcagt gaagtcggcc cctaggaaca ccagcactat ggccgtgaac gcgaacagcc
     1141 ccacgtatag ggtaaggaac gccgcgacgg cccgcagggc gtcctcgggg atgatgcggc
     1201 cgccgatgcg gatcggcatg acggcgcgcg gatgcagcgc ccgccgcact tcgcggcccg
     1261 tgatcttggc gatcatcagc cagcgcagca ccttcacccc gccgctggcg gagccggcgc
     1321 tgcctcctac gaacatgagc gtgaggagca cggcttgcgc cggcg
//
LOCUS       DAOQYE010000727         1364 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_124205_length_1364_cov_2.755539, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000727 DAOQYE010000000
VERSION     DAOQYE010000727.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124205_length_1364_cov_2.755539"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="PKN52_10555"
     CDS             complement(<1..594)
                     /locus_tag="PKN52_10555"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNR00435.1"
                     /translation="MSATVSTAGPARGASRFRRLRAFSRHRLGLVGLVIILVFTLIAF
                     FAPQIAPYDPTTQRIATARLVPPGGEFWFGTDELGRDLFSRIVYGARISMRIGIIAEG
                     IALLVGITLGAAAGFFGGWIDNLVMRIADVFFAIPGLLFLIVVVAIFGSSATTIFLAL
                     GLISWPGEARVMRSEVLRVREREYVVAAKALGLRDFGI"
     gene            complement(591..>1364)
                     /locus_tag="PKN52_10560"
     CDS             complement(591..>1364)
                     /locus_tag="PKN52_10560"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNR00436.1"
                     /translation="LGLNDPWYAQYGRFLYRTVMGDLGKSFVLDEQVGDIIGYRFPRT
                     LQLMSAALLVAILIGIPSGILAARHQYTWIDHTLMLVALLGVAMPVFWQAVLAKIFLT
                     QDTYGVALFPVAGYGGGNVRYLVLPALVLGTHLSATIARVMRSSLLEERRKDYSRTAR
                     SKGLGEPGVIFRHELRNAMIPVVTIIGLDVGYLLGGSVVTETVFNWPGLGRAVVTAIS
                     RRDAPVIIGTLVFGALLFVAVNILVDIFYAVINPQIRYA"
BASE COUNT          263 a          420 c          500 g          181 t
ORIGIN      
        1 gatgccgaag tcgcgcagtc cgagcgcctt cgcggccacg acgtactccc gttccctgac
       61 cctcagcact tccgagcgca tgacgcgtgc ctcacccggc caactgatga gcccgagcgc
      121 gaggaagatc gtggtggcgc tcgagccgaa gatggcgacg accacgatga ggaagagcag
      181 ccccgggatg gcgaagaaca cgtccgcgat gcgcatgacg aggttgtcta tccaaccccc
      241 gaagaagccg gcggccgcgc cgagcgtgat gccgaccagg agcgcgatcc cctcggcgat
      301 gatcccgatg cgcatgctga tgcgcgcgcc gtagacgata cgggagaaga ggtcgcggcc
      361 gagctcgtcc gtgccgaacc agaactcgcc gccgggcggt acgagcctgg cggtagcgat
      421 gcgctgcgtc gtggggtcgt aaggggcgat ctgcggggcg aagaacgcta tgagcgtgaa
      481 caccaggatg atcaccaacc ccacgaggcc gaggcggtgc ctggagaagg cgcgaaggcg
      541 gcggaagcgg gacgcgccgc gcgctgggcc tgcggtggag acggtggcgc tcatgcgtac
      601 ctgatctgcg ggttgatcac cgcgtagaag atatcgacga ggatgttgac tgccacgaag
      661 agcagcgccc cgaagacgag agtgccgatg atgacggggg cgtcgcggcg cgagatggcc
      721 gtgacgacgg cgcggccgag acccggccag ttgaagacgg tctcggtcac gacggagccg
      781 ccgaggagat aaccgacgtc gagaccgatg atggtcacga cggggatcat ggcgttgcgg
      841 agttcgtgcc ggaagatcac gcccggctcg ccgaggccct tggagcgtgc cgtgcgcgag
      901 tagtccttgc gccgctcctc gaggagcgag gagcgcatga cgcgcgcgat ggtcgccgac
      961 aggtgcgtgc ccagcaccaa cgccggcagc accaggtagc ggacgttgcc gcccccgtag
     1021 cccgcgacgg ggaacagcgc cacgccgtac gtgtcctgcg tcaggaagat cttggcgagg
     1081 accgcctgcc agaagacggg catcgccacg cccaggaggg ccaccagcat gagcgtgtgg
     1141 tcgatccagg tgtactggtg acgcgcggca aggatgccgg agggtatgcc gatgaggatg
     1201 gccaccaaca gggccgccga catgagctgc agcgtgcgcg ggaagcggta gccgatgatg
     1261 tcgcccacct gctcgtcgag cacgaagctc ttgccgaggt cgcccatcac ggtgcggtac
     1321 aagaaccttc cgtactgggc gtaccacggg tcgttgaggc cgag
//
LOCUS       DAOQYE010000728         1361 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_124702_length_1361_cov_2.256508, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000728 DAOQYE010000000
VERSION     DAOQYE010000728.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1361
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124702_length_1361_cov_2.256508"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="PKN52_10565"
     CDS             complement(<1..396)
                     /locus_tag="PKN52_10565"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HNR00437.1"
                     /translation="MSHRTSTRRGAVTLMVGTLASRVTGLLKNSLLAQFFPTAVIDAF
                     VTAFKVPNLFRELLAEGALTNSFIPVYKRLDRDDARRMAGALLALLLVVNGLLLIGAY
                     LGAPLVARLLISGAGNVDLELTTRLVRVVF"
     gene            471..1058
                     /locus_tag="PKN52_10570"
     CDS             471..1058
                     /locus_tag="PKN52_10570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00438.1"
                     /translation="MVDDTHHTTRVSLTAQYDHPSKARDAAAALEAAGFDKGDVKVVA
                     GASDEILRAQRQSDPYVLQRDEVPPATGAAMGFVVGLLGFGFLGLLTGSGLVNIMGKE
                     AAQAVGPFWAAVIAGGIGGLGLALAGYIFNAPLPLPEPPEHAPGKPDPRTFVSVLTEP
                     GKQAAAGAALSRFEPGLLTEWQADNGEWLPQHTRA"
     gene            1080..>1361
                     /locus_tag="PKN52_10575"
     CDS             1080..>1361
                     /locus_tag="PKN52_10575"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNR00439.1"
                     /translation="MRYLVTGSAGFIGYHLAERLLSQGAEVVGVDNLNDYYDVRLKEA
                     RLERLAGHARHRFEHVDLADAKELNRVFDEFRPEIVVNLAAQAGVRYSLS"
BASE COUNT          222 a          474 c          470 g          195 t
ORIGIN      
        1 gaacacgacg cgcacgaggc gcgtggtcag ctcgaggtcg acgttgcccg cgccggagat
       61 caggagccgc gccacgagcg gggcgcccag gtaggcgccg atgagcagga ggccgttcac
      121 gacgaggagc agagcgagga gcgcgcccgc catgcgccgc gcgtcgtcgc ggtcgaggcg
      181 cttgtacacc gggatgaacg agttggtgag cgccccctcc gcgaggagct cgcggaacag
      241 gttcgggacc ttgaaggccg tgacgaacgc gtcgatgacg gcggtcggga agaactgcgc
      301 cagcaaggag ttcttgagga gccccgtgac gcggctggcg agcgtgccga ccatgagggt
      361 cacggcgccg cggcgggtgc tggtcctgtg gctcacgcgc gccatcatcg catcatggcg
      421 ccgcgaaccg ccccggcgct atcatgtacg ccagctcgga aggagcttcc atggtcgacg
      481 acacccatca caccacgcgc gtcagcctga ccgcgcagta cgaccacccc tccaaggcgc
      541 gcgacgccgc ggcggccctc gaagccgccg ggttcgacaa gggcgacgtg aaggtggtgg
      601 ccggcgcctc ggacgagatc ctgcgggccc aacgccagtc cgacccgtac gtgctccagc
      661 gcgacgaggt cccgcctgcc accggcgcgg ccatggggtt cgtcgtcggg ctcctcggct
      721 tcggcttcct cggcctgctc acgggctccg gccttgtgaa catcatgggc aaggaggccg
      781 cccaggcggt cggcccgttc tgggcggccg tcatcgccgg cggcatcggc gggctcggcc
      841 tggctctggc cggctacatc ttcaacgccc cgctgccgct gcccgaaccg ccggagcacg
      901 cccccggcaa gccggacccg cgcaccttcg tctccgtgct cacagagccg ggcaagcagg
      961 cggcggccgg cgccgccctg agccgtttcg aacccggtct cctgaccgaa tggcaggccg
     1021 acaacggtga gtggttgccg cagcacactc gggcctagct gctgagccat gatcgacgca
     1081 tgcgctatct agtcaccggc agcgccgggt tcatcggtta ccacctcgcg gaacgcttgc
     1141 tctcccaagg cgccgaggtc gtaggggtcg acaatctcaa cgattactac gatgtgcgcc
     1201 tcaaggaggc gcggctggaa agactggccg gtcatgcccg gcaccgcttc gagcatgtcg
     1261 acttagccga cgccaaagag ttgaaccgcg tgttcgacga gttccgacca gagatcgtcg
     1321 tgaacctagc cgctcaggcg ggcgtgcgct attcgctcag c
//
LOCUS       DAOQYE010000729         1358 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_125161_length_1358_cov_2.360706, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000729 DAOQYE010000000
VERSION     DAOQYE010000729.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1358
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125161_length_1358_cov_2.360706"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="PKN52_10580"
     CDS             complement(<1..342)
                     /locus_tag="PKN52_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HNR00440.1"
                     /translation="MSVQRAELLYEGKAKKVYATTDPTRHVIEHKDDATAFNAQKRGT
                     IAGKGEVNAAVASRVFEFLAEAGVPTHLEALISPREQLVKAVRIVPLEVVVRNRAAGS
                     FARHCRTSSESA"
     gene            complement(460..1179)
                     /locus_tag="PKN52_10585"
     CDS             complement(460..1179)
                     /locus_tag="PKN52_10585"
                     /inference="COORDINATES: protein motif:HMM:NF012432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HNR00441.1"
                     /translation="MPVACHRPVAAALGLTPDPITNQTVARDRHAEVMAALAILGTSI
                     ERVALEIRHLQRTEVREAQEGFARGQTGSSSMPHKKNPIASENLTGVARVMRANLQAA
                     LENVALWHERDISHSAAERIILPDSTTLAVYALRRLASVLRGLVVYPERMGENLDALR
                     GLVYSQRVLHLLIEAGMSREAAYAIVQRASLRCWESGEHLRDLLAAEAGLPLSPDELD
                     GAFDPAWYLRYVDRIFEANGL"
     gene            complement(1134..>1358)
                     /locus_tag="PKN52_10590"
     CDS             complement(1134..>1358)
                     /locus_tag="PKN52_10590"
                     /inference="COORDINATES: protein motif:HMM:NF012432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HNR00442.1"
                     /translation="AQNLLLGRAITIVLADVAAVKERLLELAQRYRHTPCIGRTHGIH
                     AEPMTFGLKFLNFTSACRSRATVRSLPPSA"
BASE COUNT          191 a          457 c          501 g          209 t
ORIGIN      
        1 cgcgctctcc gagctcgtgc gacagtgcct ggcgaaggag ccggccgcgc ggttgcgcac
       61 gacgacctcg aggggcacga tccgtacggc cttgacgagc tgctcgcggg gcgatatgag
      121 cgcctcgagg tgcgtaggaa cgcccgcctc ggcgaggaac tcgaaaacgc gcgaggccac
      181 ggcggcgttg acctcgccct tgccggcgat ggtgccgcgc ttctgcgcgt tgaaggccgt
      241 ggcgtcgtcc ttgtgctcga tgacgtgccg cgtcgggtcg gtggtcgcgt agaccttctt
      301 ggccttgcct tcgtacagca gttcagccct ctggacgctc acggccagat gatagcaggg
      361 ggtcggtggc ccgtcgcctc cgccaggagt cgctccagag cggccggccg ggcgccgcgc
      421 aggcgcctgt ccgggcgact cgcactgccg gacgcgcgcc tacagcccgt tcgcctcgaa
      481 gatgcggtca acgtaacgca ggtaccaggc ggggtcgaac gcgccgtcca gctcatctgg
      541 gctcaacggg agtccggctt ccgccgcgag caggtcgcgc aagtgctcgc ccgactccca
      601 gcagcgcagc gaggcccgct ggacgatcgc gtaggcggcc tcgcggctca tgccggcctc
      661 gatgaggagg tggaggaccc gctgcgagta gacgagaccc ctgagggcgt ccaggttctc
      721 gcccatccgc tccgggtaga cgaccaggcc gcgcaggacg gaggccaggc gcctcagggc
      781 gtacacggcc agcgtggtgg agtccgggag gatgatccgc tccgccgccg aatggctgat
      841 gtcgcgctcg tgccagagcg ccacgttctc cagagcagcc tgcaggttgg cgcgcatgac
      901 gcgcgccacg cccgtcaggt tctccgaagc gatcgggttc ttcttgtgcg gcatcgacga
      961 cgagcccgtc tggccgcggg cgaacccctc ctgcgcctcg cgcacctccg tgcgttggag
     1021 gtgcctgatc tcgagggcga cgcgctcgat gctcgtgccc aggatggcga gcgccgccat
     1081 cacctccgcg tgccggtcgc gtgccaccgt ctggttggtg atggggtcgg gcgtcaggcc
     1141 gagggcggca gcgaccggac ggtggcacgc gaccggcacg cggaggtgaa gttgaggaac
     1201 ttcagcccga acgtcatggg ctcggcgtgg atgccgtgcg tgcgaccgat gcacggcgtg
     1261 tgccggtagc gctgggcgag ttcgaggagc cgctccttca cagccgccac gtcggccagg
     1321 acgatcgtga tggcgcggcc gagcagcagg ttctgggc
//
LOCUS       DAOQYE010000730         1358 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_125201_length_1358_cov_1.957022, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000730 DAOQYE010000000
VERSION     DAOQYE010000730.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1358
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125201_length_1358_cov_1.957022"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="PKN52_10595"
     CDS             <1..191
                     /locus_tag="PKN52_10595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00443.1"
                     /translation="APEDLDGDGSVGFYDLVAFGQAYGRTGVNLRADFNGDGRVDDAD
                     LAVLRAAYEFGPPQPTPP"
     gene            complement(250..>1358)
                     /locus_tag="PKN52_10600"
     CDS             complement(250..>1358)
                     /locus_tag="PKN52_10600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00444.1"
                     /translation="QLRLALQELGKERLPEEEKAAQRRELERRLSELTARERADRQSF
                     QRDTAHFVELVEAFFGRLAGYLPTTVGGEAGGPHLEGGVLFGVNPILKWERVPAEASA
                     VTVRMVGPVRLTLAGHDLAIGGVGNDRTLYLDGEAVDLDGSRVLRRDRVRIGAFREGE
                     YVHVRIRNDAKSLGARLADALVVNEVLASPQRDELLATMKVLANNVRGEVDEVVGQAV
                     ARANVVALRAPSRMKALEGLLRGAAKAAGVVVSDDTVGNVVARMAKVGTVEPSDLPGL
                     VATSGASEHVVKTLTQEPMTFDLANLKITVRQYRGRDRAAQESLVAMLPGQVLGSFTE
                     SMLTPRPGGTLIFARGEEEVAVLFVPNRPDGTAY"
BASE COUNT          177 a          530 c          461 g          190 t
ORIGIN      
        1 gcgctcccga ggacctcgac ggcgacggca gcgtgggctt ctacgacctg gtggcgttcg
       61 ggcaggcgta cggccggacg ggcgtgaacc tccgcgccga cttcaacggc gacggccgcg
      121 tggacgacgc cgacctggcg gtgttgcgcg cggcgtacga gttcggcccg ccgcaaccga
      181 cgcccccctg agcttcccgc cggcgctccc catcggggcg gccgcgggcg cgcgcaggca
      241 aacgggcggt cagtaggcgg tcccgtcggg gcggttcggg acgaacagga ccgccacctc
      301 ctcctcgccg cgcgcgaaga tcaacgtccc gccaggccgc ggcgtgagca tcgattccgt
      361 gaacgacccg agcacctggc ccgggagcat ggcgaccagg ctctcctgcg cggcgcggtc
      421 ccggccgcgg tactggcgga ccgtgatctt gaggttggcg aggtcgaacg tcatcggctc
      481 ctgagtcagc gtcttcacga cgtgctcgct cgcccccgac gtggccacga ggcctggcag
      541 gtcggacggc tcgaccgttc ccaccttcgc catgcgcgcc acgacgttgc cgaccgtgtc
      601 gtcgctcacc acgacgccgg ccgccttggc cgccccccgc aggagcccct ccagcgcctt
      661 catgcggctc ggtgccctga gcgccacgac gttggcgcgc gcgacggcct ggccgacgac
      721 ctcgtcgacc tcgccgcgaa cgttgttggc aagcaccttc atcgtggcga gcagttcgtc
      781 gcgttgcggc gacgccagca cctcgttcac gaccagcgcg tcggcgagac gcgcgcccaa
      841 cgacttcgcg tcgttgcgga tcctcacgtg gacgtactcg ccctcacgga acgcgccgat
      901 gcggacgcgg tcgcgcctaa ggacgcgtga cccgtcgagg tcgaccgcct cgccgtcgag
      961 gtagagcgtc cggtcgttgc ccaccccgcc tatcgccagg tcgtggcccg ccagggtgag
     1021 ccgcaccgga ccgaccatgc gcaccgtcac cgccgacgcc tccgcgggca ccctctccca
     1081 cttgaggatc gggttgaccc cgaagaggac cccgccctcg aggtgcggcc cgccggcctc
     1141 gccgcccacg gtcgtgggca ggtagccggc cagccggccg aagaacgcct cgacgagctc
     1201 cacgaagtgc gccgtgtcgc gctggaacga ctggcggtcg gcccgctccc gcgcggtgag
     1261 ctcgctgagg cgccgctcca gttcccggcg ctgcgccgcc ttctcctcct cgggcagccg
     1321 ctccttcccc agctcctgta gcgccaggcg caactggg
//
LOCUS       DAOQYE010000731         1357 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_125260_length_1357_cov_3.502304, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000731 DAOQYE010000000
VERSION     DAOQYE010000731.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1357
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125260_length_1357_cov_3.502304"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1274)
                     /locus_tag="PKN52_10605"
     CDS             complement(<1..1274)
                     /locus_tag="PKN52_10605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00445.1"
                     /translation="MTDASEPVRMGEVFRTWLPLAASWLVMAMEQPSVSAVMARLADP
                     ELNLAAYGGVVLPSALIVEAPVMMLLAASVALAKDQASHRLIYRFMMGLGLGLTVLHA
                     LLAFTPLFDLVIVPLLGPPAGVVEPARLGLKLMLPWTWAIAYRRFHQGLLIRFRRSHH
                     VTIGTALRLVTVVSAAAVLGFALGAPGVVAGTLAISIGVIVEALYSGLAALPVVRLQV
                     KRAATVTPPLTIGAFLAFFFPLLLTSLLSLLVQPIGSAALARMPEALASLAVWPVANG
                     LMFMLRSVAFALNEVVVALLDRPGAYRTLRRFTWLLGGSLLLITLAVGFTPLSRVWFV
                     NVSGLTPALASLATVGFAWALMWPALDAYRNLLQGVAVYAKRTAVISSATIIFLAVSA
                     ALLVAGILTQRFPALPVAMFSFVVATVAQVLWL"
BASE COUNT          265 a          438 c          504 g          150 t
ORIGIN      
        1 agccacagca cttgcgccac cgtggcgacc acgaacgaga acatcgcgac gggcagggcg
       61 gggaagcgct gggtcagtat gcctgccacg agcagcgccg cgctgacggc gaggaagatg
      121 atggtcgccg agctgatgac ggccgtgcgt ttggcgtaca cggcgacgcc ttgtaggagg
      181 ttccgataag cgtcgagcgc gggccacatg agcgcccagg cgaagccgac cgtggcgagc
      241 gaagccaggg ccggggtgag cccggaaacg ttcacgaacc agacccggga gaggggcgtg
      301 aagcctaccg ccaaggtgat cagcaggagc gagcctccca ggagccatgt gaagcgccgc
      361 agcgtgcggt aggcaccggg ccggtcgagc agcgcgacca ccacctcgtt gagggcgaac
      421 gccaccgacc tgagcatgaa catgaggccg ttcgcgaccg gccacacggc gagcgacgcc
      481 aacgcctcgg gcatccgcgc gagcgcggcg ctgcctatcg gctgcacgag cagtgacagt
      541 aggctcgtga ggagcaaggg gaagaagaag gcgaggaagg ccccgatggt caggggaggg
      601 gtgacggtgg cggcgcgttt gacctggagc cgcaccacgg gcaacgccgc caagccgcta
      661 tacagcgcct cgacgatgac cccgatggag atggccaggg tcccggccac gacgccgggg
      721 gcgccgagag cgaacccgag caccgcggcg gcgctgacga cggtcaccag gcgcagcgcc
      781 gtgccgatgg tgacgtggtg cgaacgtcgg aacctgatca agagaccctg gtggaaccgc
      841 cggtaggcga tcgcccaggt ccatgggagc atgagcttca agcccagcct ggccggttcg
      901 acgacgccgg ccggcggccc gagcagcggc acgatgacga ggtcgaacag gggcgtgaac
      961 gccagcagcg catgcaggac ggtcaacccc aggccgagcc ccatcatgaa ccggtagatg
     1021 agccggtggg aggcctggtc cttggcgagg gcgacgctgg cggccagcag catcatcaca
     1081 ggcgcctcga cgatcagcgc gctgggtagg acgacgcccc cataagccgc caggttgagt
     1141 tccggatccg cgaggcgcgc catcacggcg ctgacggagg gctgctccat ggccatgacc
     1201 agccaactgg cggcgagcgg cagccaggtc cggaagacct cccccatgcg gaccggctca
     1261 ctggcgtctg tcactggcga gaactgtaca tccgccgccg tggtccttgc gtggggcgca
     1321 ggcggatccg ccgctgccaa gccagccgga gcgcggc
//
LOCUS       DAOQYE010000732         1353 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_125902_length_1353_cov_2.771957, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000732 DAOQYE010000000
VERSION     DAOQYE010000732.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1353
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125902_length_1353_cov_2.771957"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1218)
                     /locus_tag="PKN52_10610"
     CDS             complement(<1..1218)
                     /locus_tag="PKN52_10610"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HNR00446.1"
                     /translation="MTGIGETSAPGGEVRVRLATDRGSAWLALTFPTDGACRLGLTRA
                     QPLHAAHGAAGRVREDEHGFIIEGPGIVLRVNRADLTLTAARPEVPDVALFSGGLRFS
                     WLERDGVASTTVVTNFQTGEGEAIYGLGERFVDADRNGQRWDVRVYEEYKEQRKRTYI
                     PIPLLVSERSYGLWLDAPEPSYLDLTERAAEWTIDRLNVEPDGSARSLLDLVVFVAEE
                     PYGVTAAFTRLTGRPAVPPTWAFGPWMSANTWNSQALVEEVVARTLAEDVPASVVVIE
                     AWSDESTFYIFNDAEYEPKANGAAHGLADFRFTGRWPDPKGMVDWCHENGVRVVLWQI
                     PVHKRLGEPHAQHDLDGQYMIDNGLAILDPDGTPYRNKGWWFTDALVADFSNPATHDF
                     WFGKRRYLFEELGI"
BASE COUNT          202 a          469 c          464 g          218 t
ORIGIN      
        1 tatgccgagc tcctcgaaca ggtagcgccg cttgccgaac cagaagtcgt gcgtggcggg
       61 gttggagaag tcggcgacga gcgcgtccgt gaaccaccag cccttgttcc ggtacggggt
      121 gccgtcaggg tcgaggatgg cgaggccgtt gtcgatcatg tactggccgt cgaggtcgtg
      181 ctgtgcgtgg ggctcgccaa gccgcttgtg aacggggatc tgccagagca cgacgcgcac
      241 gccgttctcg tgacaccagt ccaccatgcc cttggggtcg ggccacctgc ctgtgaaccg
      301 aaagtccgcg agcccgtgcg cggcgccgtt cgccttgggt tcgtactcgg cgtcgttgaa
      361 gatatagaag gtcgactcgt cgctccacgc ctcgatgacg accacggagg cgggcacgtc
      421 ctccgcgagc gtgcgcgcca cgacctcctc gaccagcgcc tgcgagttcc acgtgttggc
      481 cgacatccac ggcccgaagg cccacgtcgg cggaacggcg ggccggcccg tgaggcgcgt
      541 gaaggccgcg gtgaccccgt acggctcctc ggccacgaag acgacgaggt cgagcaggct
      601 tcgcgcggag ccgtccggct cgacgttcag ccggtcgatg gtccattcgg ccgctcgttc
      661 tgtgagatca agatagctcg gttccggagc gtcgagccac aagccgtagc tccgctccga
      721 gacgagcagc gggataggga tataggtccg cttgcgttgc tccttgtact cctcgtagac
      781 gcgcacgtcc cagcgctggc cgttgcgatc cgcgtccacg aagcgctccc cgaggccgta
      841 gatggcctcc ccctcgcccg tctggaagtt cgtgacgacg gtggtgctcg ccacgccgtc
      901 gcgctcgagc cagctgaagc gcaggccgcc cgagaacagg gcgacgtcgg gcacctccgg
      961 cctggctgcg gtgagcgtca ggtcagcacg gttcacgcgc aggacgatgc ccgggccctc
     1021 gatgatgaag ccgtgttcgt cctcgcgcac tcgaccggcc gcaccgtgcg cagcatggag
     1081 aggttgagcg cgcgtcagcc cgagtcggca agccccgtcc gtcgggaacg tgagcgcgag
     1141 ccacgcgctg ccgcggtcgg tagcgagcct gacccgcacc tcgcctcccg gtgcgctggt
     1201 ctcacctatc cccgtcacgg tcgtccatga gccgacgtcg aagcgatatg ggccggcgct
     1261 gatggggtcg tccgcggccc tcgatgccgg gcctgatccg ggatcttccc cggcgcgcga
     1321 cccggagctc gaccacgcgc ccgacccgac gtc
//
LOCUS       DAOQYE010000733         1353 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_125963_length_1353_cov_2.059322, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000733 DAOQYE010000000
VERSION     DAOQYE010000733.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1353
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125963_length_1353_cov_2.059322"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..987)
                     /locus_tag="PKN52_10615"
     CDS             complement(<1..987)
                     /locus_tag="PKN52_10615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNR00447.1"
                     /translation="MVAARRSEFLGLLRLAAPLVLAQLAQNGLSFVDTLMVGRLGAGA
                     LAAMALGGSFYFTTGVITSSVLYAVGPVVAQAIGAGRRAEAGHAASRALWLAVALSIP
                     GLVIFNAIGPVLVRFGLEPETARLAAGYLRAISFGFPFYLGFAALRGFLEGHGDARPI
                     MYIAFFGVALNVVLCEVLIFGHLGFPALGVIGTGYATATVYTVMFALVAALVVWRYPR
                     QRVFAGMADVRPRMLGELVRLGWPIAFTTGMEVGLFSLSSLIIARFGDSVLAAHQVAM
                     QSASMTFMVPLGVAIATGVIVGQAAGAGDMAKVRRTGFMGIGVAVGFMLCSAT"
     gene            1047..>1353
                     /locus_tag="PKN52_10620"
     CDS             1047..>1353
                     /locus_tag="PKN52_10620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00448.1"
                     /translation="MRIVIALLVVLLLAAGGLWALGLPPFKSHPREPAKPDLFAAIAS
                     ADPSAVAAAITTALAGGADVHARDLSGYTALMVAVRENAPTAALDALLTGGAALDDVT
                     "
BASE COUNT          245 a          483 c          474 g          151 t
ORIGIN      
        1 cgtcgcggag cacagcatga acccgaccgc gaccccaatc cccatgaagc cagtgcgccg
       61 caccttggcc atgtcgcctg cgccggcggc ctgcccgacg atcacgccgg tggcgatggc
      121 cacgccgagc ggcaccatga aggtcatcga ggccgactgc atggcgacct ggtgcgcggc
      181 gagcaccgaa tccccgaagc gggcgatgat caggctggag aggctgaaga ggcccacctc
      241 catgccggtc gtgaacgcga tcggccagcc cagcctgacg agttcgccaa gcatgcgtgg
      301 ccggacatcg gccatgccgg cgaagacacg ctgccggggg tagcgccaga cgacgagcgc
      361 ggccaccagg gcgaacatga ccgtgtacac cgtcgccgtc gcgtagcccg tgccgatcac
      421 cccgagcgcc gggaagccga gatggccgaa gatcagcacc tcgcacagca ccacgttgag
      481 ggcgacgccg aagaacgcga tgtacatgat cgggcgcgcg tcgccgtgcc cctccaggaa
      541 gccccgcaag gccgcgaagc ccaggtagaa agggaaaccg aacgagatcg ccctcaagta
      601 gccggcggca aggcgcgccg tctccggctc gagcccgaag cgcacgagga cgggaccgat
      661 ggcgttgaag atgaccaggc cggggatcga aagcgcgacg gccagccaga gcgcccgcga
      721 cgcggcgtgc cctgcctcgg ctcgccttcc ggcgccgatg gcctgagcca cgacgggtcc
      781 gaccgcgtat agcacggagg acgtgatgac gccggtcgtg aagtagaagc tgccgccgag
      841 cgccatggcg gcgagggcgc cggcgccgag gcgcccgacc atgagagtgt caacgaagct
      901 caggccgttc tgcgccagtt gggcgagaac gaggggcgcc gccaggcgca gtaggccaag
      961 gaactcgctt ctgcgcgcgg cgaccacgcc caccaagata cccgtgcggg gcaagcaagg
     1021 cgccgggccg cggtagcatg gccggcatgc ggatcgtcat cgcactgctc gtggtcctgc
     1081 tgctggccgc cggtgggctc tgggcgctcg gcctgccgcc cttcaagagc catccgcgcg
     1141 agccggcgaa gcccgacctg ttcgccgcga tcgcgtcggc cgaccctagc gccgtggcgg
     1201 ccgccataac cacggccctc gcaggtggcg cggacgtgca cgccagagac ctcagcggct
     1261 acacggccct catggtggcc gtcagggaga acgcccccac cgccgccctc gacgcgctcc
     1321 ttaccggcgg cgcggcactg gacgacgtta cgg
//
LOCUS       DAOQYE010000734         1350 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_126426_length_1350_cov_2.129730, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000734 DAOQYE010000000
VERSION     DAOQYE010000734.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126426_length_1350_cov_2.129730"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="PKN52_10625"
     CDS             <1..298
                     /locus_tag="PKN52_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="deaminase"
                     /protein_id="HNR00449.1"
                     /translation="WRLTGCTLYVTLEPCAMCFGAVLQSHVARVVFGAPNLRDGALGG
                     VADVSLAPWKRVPAILGGVEGGAAARLLKDAFAAARGADAPEGGTDDPRQDGMN"
     gene            327..>1350
                     /locus_tag="PKN52_10630"
     CDS             327..>1350
                     /locus_tag="PKN52_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNR00450.1"
                     /translation="MEIRNFSIIAHVDHGKSTLADRILELTESVSERDRRDQMLDSLE
                     LERERGITIKATPMRLYYTSDSGQPYQFNLIDTPGHVDFNYEVSRALGACEGVLLVID
                     AAQGVEAQTIQNAYLAADNGLEILPVINKIDLPAADVPGAIAALEEIIGVPGDHAVPV
                     SAKTGENVHAVLEAVVRHLPGPKGERDAPLRALIFDAVYDAYQGVIAFIRVFDGVIRS
                     KDRVLIHSTGKVLEVERVGFFRPEPTVAAELGVGEVGWFTAGIKEIADTQIGDTVTSA
                     DRPTDRPVPGFKPAMPVVFSGLYPTDTEDYPRLREALEKLRLNDAAFSFEPETSEALG
                     FGFRCGF"
BASE COUNT          215 a          494 c          440 g          201 t
ORIGIN      
        1 ttggcggctc acgggctgca cgctctacgt gacgctcgag ccctgcgcga tgtgcttcgg
       61 cgccgtgctc cagtcgcacg ttgcgcgcgt cgtcttcggc gcccccaacc tcagggacgg
      121 cgcgctcgga ggcgtcgccg acgtctcgct cgcgccttgg aaacgggtcc cggcgatcct
      181 tgggggcgtc gagggtggcg cggcggcacg gctcctcaag gacgccttcg cggcggccag
      241 gggcgcagac gccccggaag gcggcaccga tgacccccgc caggacggca tgaactgaac
      301 gcttggggtt acgctagact acccgggtgg agatccgcaa cttctcgatc atcgctcacg
      361 tcgaccacgg caagtcgacg ctggccgata ggatcctgga actgacagag agcgtctcgg
      421 agcgcgacag gcgcgaccag atgctcgatt ccctcgagct cgagcgcgag cgcggcatca
      481 cgatcaaggc cacgcccatg cgcctctact acacgtccga cagcgggcag ccctaccagt
      541 tcaacctcat cgacacgcct ggccacgtcg acttcaacta cgaggtctcc cgcgccctcg
      601 gcgcctgcga gggcgtgctg ctcgtgatag acgcggcgca gggggtcgag gctcagacca
      661 tccagaacgc ctacctggcg gccgacaacg gcctcgagat cctccccgtc atcaacaaga
      721 tcgacctgcc ggcggccgac gtgcccggcg ccatcgccgc actcgaggag atcatcggcg
      781 tgcccggcga ccatgcggtg cccgtatcgg ccaagacggg ggagaacgtg cacgccgtcc
      841 tcgaggccgt ggtcaggcac ctgcccggtc cgaagggcga gcgcgacgcg cccctgcgcg
      901 ccctcatctt cgacgccgtc tacgacgcgt accagggcgt catcgcgttc atccgcgtct
      961 tcgacggcgt catcaggtcg aaggaccgcg tcctcatcca ctcgaccggc aaggtgctcg
     1021 aggtcgagcg ggtcggcttc ttccggcccg agccgaccgt cgcggccgaa ctcggcgtcg
     1081 gcgaggtcgg ttggttcacg gccggcatca aggagatagc cgacacgcag atcggcgaca
     1141 ccgtcaccag cgccgaccgc ccgaccgaca ggcccgtgcc gggcttcaag cccgccatgc
     1201 cagtggtgtt ctccggcctc tacccgaccg acaccgagga ctacccgcgc ctgcgcgagg
     1261 ccctcgagaa gctgcgcctc aacgacgcgg cgttctcgtt cgagccggag acgtccgagg
     1321 ccctcggctt cgggttccgt tgcggcttcc
//
LOCUS       DAOQYE010000735         1349 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_126571_length_1349_cov_2.244977, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000735 DAOQYE010000000
VERSION     DAOQYE010000735.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126571_length_1349_cov_2.244977"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="PKN52_10635"
     CDS             complement(<1..400)
                     /locus_tag="PKN52_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNR00451.1"
                     /translation="MPSGSDRAPVGKAQDAFTKAFQFRRADEAVSMGMHPYFKAIASQ
                     HGGTVTVGGREMVITGSNDYLGLTQDPRLKEAARRALDDFGTSCTGSRFLTGTLTLHE
                     ELEQRLAVFFQREAVLTFSAGFLGCLSVVSA"
     gene            659..1159
                     /locus_tag="PKN52_10640"
     CDS             659..1159
                     /locus_tag="PKN52_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNR00452.1"
                     /translation="MRTRAASAADAATIQELYRATPGYFDVISIPVPTVDEVRTDLAT
                     ATGDPRRHVEIVLIEPERVGEAATIDPVTGEAAVGYLDYKLDYPEEGDATVNLMLVRG
                     DMQSRGIGRACALDLEERLKGRSRRMLASIYGENPRARAFWESLGYRFAIDARPLLEW
                     YAKRLA"
     gene            1156..>1349
                     /locus_tag="PKN52_10645"
     CDS             1156..>1349
                     /locus_tag="PKN52_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00453.1"
                     /translation="MSGGVLDVVSVSLGSVKRDTDQEVEVLGRRVRLRRVGTGGDLTA
                     ARTMIGELDGKVDAIGLGGI"
BASE COUNT          211 a          461 c          474 g          203 t
ORIGIN      
        1 aggcggagac gacggagagg cagccgagga acccggcgct gaatgtgagc acggcttcgc
       61 gctggaagaa gacggccagg cgctgctcca actcctcgtg gagcgtgagg gtccccgtca
      121 ggaagcgcga gcccgtgcaa ctcgtgccga agtcgtcgag cgcgcgcctc gccgcctcct
      181 tgaggcgcgg gtcctgggtg agacccaggt agtcgttcga gcccgtgatc accatctcgc
      241 ggccgccgac cgtgacggtg ccgccgtgct gcgaggcgat ggccttgaag taggggtgca
      301 tgcccatgct cacggcctcg tccgccctgc ggaactggaa ggccttcgtg aacgcgtcct
      361 gcgccttgcc taccggcgcc ctgtcacttc cgctgggcat gacctctgac aaaatctcct
      421 ccatgtgatc cgcttgctcc gggcgatcct agcatcccgg gcggtaccgg gctagccgag
      481 ccgcccacgc gcgctcgtga agcgacctgt gagcgacccg acacgtcggt tcacaggtgc
      541 gcgttggtag aatcctccgg tgtcccaacg tggcgacgcg ctcgcagtcc cgccccaggc
      601 cggtggcgcg acctgggcgc ccgaagaggt gaccggtttg cttcctacac tctccccggt
      661 gcgcacgcgc gccgcttcgg ccgccgacgc ggccaccatc caagaactct accgggcgac
      721 ccccgggtac ttcgacgtca tctccatccc ggtgccgacg gtggacgagg tccgcacgga
      781 cctggcgacg gccacgggcg acccgcgcag gcacgtcgag atcgtgctga tcgagcccga
      841 gcgtgtcggc gaggcggcga ccatcgatcc cgtgacgggc gaggcggccg tcggttacct
      901 cgactacaag ctcgactacc cggaggaggg ggacgcgacc gtcaacctga tgctcgtgcg
      961 aggcgacatg cagagccggg gcatcggccg ggcctgcgcc ctcgacctgg aggagcgcct
     1021 caagggacgc agccggcgga tgctcgcgag catctacggt gagaacccgc gtgccagggc
     1081 gttctgggag agcctaggct accgtttcgc catagacgcg cggccgctcc ttgagtggta
     1141 cgccaagcgg ttggcatgag cggcggagtc ctggacgtcg tcagcgtgtc gctcggctcc
     1201 gtcaagcgcg acactgatca ggaggtcgag gtgctcggca ggcgcgtgcg cctcaggcgc
     1261 gtcggcacgg gcggcgacct cacggcggca cggaccatga tcggggagct tgacggcaag
     1321 gtcgacgcca tcggcctcgg cggcatcga
//
LOCUS       DAOQYE010000736         1346 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_126990_length_1346_cov_3.374129, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000736 DAOQYE010000000
VERSION     DAOQYE010000736.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1346
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126990_length_1346_cov_3.374129"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            151..498
                     /locus_tag="PKN52_10650"
     CDS             151..498
                     /locus_tag="PKN52_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HNR00454.1"
                     /translation="MYLEVLMMFLAAGLIAAAALVVGGLLGPKKPGEVKLSAYESGVP
                     AVGSARERFPVHFYLVAMVFIIFDLETAFFYPLAVKFQAAPQFLFANAVIFVAILAVG
                     YVYLLRKGVLNWK"
     gene            501..1037
                     /locus_tag="PKN52_10655"
     CDS             501..1037
                     /locus_tag="PKN52_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HNR00455.1"
                     /translation="MGLLELFEKDVQELEAEGILFTSLNKLVAWGRSNSVWPATFGLA
                     CCAIEMMASTNPRNDMARLGSEVFRASPRQADVMIVAGRLSKKMAPIMRRVYDQMPDP
                     KWVISMGACASSGGMFNNYAIVQNVDSVVPVDIYVPGCPPRPEALIYAVMQLQKKIRG
                     EAFDARGEQLAPVEGWRR"
     gene            1034..>1346
                     /locus_tag="PKN52_10660"
     CDS             1034..>1346
                     /locus_tag="PKN52_10660"
                     /inference="COORDINATES: protein motif:HMM:NF012549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HNR00456.1"
                     /translation="MSDSLVADNVPLGANRDKALPALVELGGVVSDGKDMINVTVPKT
                     KIVEALRACRAAGLEMLTDIFGIDYLTYPGHRGKRFAVVYNVHDVRGTDRLFVRVEVE
                     DG"
BASE COUNT          220 a          430 c          454 g          242 t
ORIGIN      
        1 ccgcctatgc ggcgctgcta cactatcgaa agtcggttgt gaaaacgcgc acgagcggcg
       61 gcgcgtcgtc acgccaaagg tcgtaagcgg tcgcttaggt ggcgcctacc agtcaaggta
      121 cccggcgccg ggcggaagga gcagcctccc ttgtatctag aggtattgat gatgttcttg
      181 gcggccgggc tcatcgcggc tgccgcccta gtggtcggcg ggctcctcgg tccgaagaag
      241 ccgggcgagg tgaagctgtc cgcctacgag tcgggcgtgc cggcggtcgg gagcgcgcgt
      301 gagcgcttcc cggtgcactt ctacctggtg gcgatggtct tcatcatctt cgacctggag
      361 accgctttct tctacccgct cgcggtgaag ttccaggccg cgccgcagtt cctgttcgcg
      421 aacgccgtca tcttcgtggc gatccttgcc gtcgggtacg tgtacctgct gcgcaagggc
      481 gtcctgaact ggaagtgagc atgggtctcc tggagctttt cgagaaggac gtgcaggagt
      541 tggaggcaga ggggatcctc ttcacgagcc tcaacaagct cgtcgcgtgg ggccgctcca
      601 acagcgtgtg gcccgccacg ttcgggttgg catgctgcgc gatcgagatg atggcgtcca
      661 cgaacccccg taacgacatg gcgcgtctcg gctccgaggt gttccgcgcg agccctcgtc
      721 aggccgacgt gatgatcgtg gccgggcgcc tctccaagaa gatggccccc atcatgcgcc
      781 gtgtctacga ccagatgccc gacccgaagt gggtcatcag catgggcgct tgcgcgtcgt
      841 ccggcggcat gttcaacaac tacgccatcg tgcagaacgt cgactccgta gtgccggtcg
      901 acatctacgt gcccggctgc ccgccgcggc ccgaggcgct catctacgcc gtcatgcaac
      961 tccagaagaa gatccgcggc gaggcgttcg acgcgcgcgg tgagcagctc gcgccggtcg
     1021 aggggtggcg cagatgagcg actcgttggt cgcggacaac gtcccgctag gcgccaaccg
     1081 cgacaaggcg ctcccggcgc tcgtcgaact cggcggggtc gtgagcgatg gcaaggacat
     1141 gatcaacgtc acggtcccga agacgaagat cgtggaggcg ctccgggcct gccgcgcggc
     1201 tgggctcgag atgctcaccg acatcttcgg catcgactac ctcacgtacc ccggtcaccg
     1261 cggcaaacgc ttcgccgtcg tctacaacgt gcacgacgtg cgtggcaccg acaggctctt
     1321 cgtgcgcgtc gaggtggagg acggcg
//
LOCUS       DAOQYE010000737         1345 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_127189_length_1345_cov_2.527907, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000737 DAOQYE010000000
VERSION     DAOQYE010000737.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1345
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127189_length_1345_cov_2.527907"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..669)
                     /locus_tag="PKN52_10665"
     CDS             complement(<1..669)
                     /locus_tag="PKN52_10665"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNR00457.1"
                     /translation="MTFRQLALSNIRGSALRYAAFFLSSTFSVTLFFVYAQFIMHPDV
                     AGGYIYGGDATRIVLTVCEVLVAVFAFFFVLYSSGAFLRARNQEFGLLSLMGTTRGQL
                     RRLIWLENSILSVGAIALGIGLGLLLSRLFLLGISRVLGLDEPIRFLFVPNAILLTAV
                     SFFALFQLVTLVGGLRIGRQEVVELMRAARKPRTVPRASPLLAVVGAALVVAGYVVAT
                     RVEGA"
     gene            complement(644..>1345)
                     /locus_tag="PKN52_10670"
     CDS             complement(644..>1345)
                     /locus_tag="PKN52_10670"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNR00458.1"
                     /translation="DGFDLRILPGEFLGVMGPSGSGKTTLLNMLATIDSPTAGSLRIA
                     GADPSGMRPTELALFRRRRLGFVFQEFNLLDTLTVRENILLPLVLDNVRVAEMEHRLR
                     AVTERLGIGALLERRPYEISGGQQQRVAIARAIIPGPDLVLADELTGNLDSKSALDVM
                     RTLQGLNDEGITVVMVTHDPFAASFCKRIVFIKDGKRFGELVRGVNRQAFFQQVLDAL
                     SVMGGGADDVQAARA"
BASE COUNT          255 a          422 c          485 g          183 t
ORIGIN      
        1 ggctccctcc acccgcgtgg cgacgacgta gccggccacg accagcgcgg cccccaccac
       61 cgcgagcagc ggcgaggcgc gcggcacggt gcgcggcttc cgggcggccc gcatgagttc
      121 gacgacctcc tgccggccga tccggagacc gccgaccaag gtgacgagct ggaacagcgc
      181 gaagaagctg acggccgtga gaaggatggc gttcggcacg aaaaggaacc ggatgggctc
      241 gtccagacct aggacccgac tgatgccaag caggaacagg cgcgagagca ggagccccag
      301 gccgatgccg agagcgatgg cacccaccga caggatggag ttctcgagcc agatgagccg
      361 cctgagctga ccgcgggtcg tgcccatgag cgagaggagc ccgaactcct ggttgcgggc
      421 acgcaggaag gcgccgctgg agtagagcac gaagaagaac gcgaagacgg ctacgagcac
      481 ttcgcagacg gtgagcacga tccgggtcgc gtcgccaccg tagatgtagc cgccggccac
      541 gtccgggtgc atgatgaact gcgcgtagac gaagaacagg gtgacagaga acgtgctgct
      601 gaggaagaag gccgcgtagc ggagggcgct gcccctgatg ttgctaagcg cgagctgcct
      661 gaacgtcatc ggcgccgcct cccatcacgc tgagggcgtc gagcacctgc tggaagaacg
      721 cctgccggtt cacgccgcgc accagctccc cgaaacgctt gccgtccttg atgaagacga
      781 tgcgcttgca gaagctcgcg gcgaacgggt cgtgggtgac catcacgacg gtgatgcctt
      841 cgtcgttcag cccctgcaac gtgcgcatga cgtcgagggc gctcttggag tcgaggttgc
      901 cggtcagctc atcggccaac acgaggtcgg gccccgggat gatggcgcgg gcgatggcca
      961 cgcgctgctg ctgaccgccg gagatctcgt aagggcgccg ctccagcaaa gcgccgatgc
     1021 cgaggcgctc cgtgaccgcc cgtagacggt gctccatctc ggcgaccctc acgttgtcga
     1081 ggacgagtgg caggaggatg ttctcgcgca cggtgagggt gtcgaggagg ttgaactcct
     1141 ggaacacgaa gccgagccgg cggcggcgga agagcgcgag ctcggtcggt cgcatgccgc
     1201 tcgggtccgc gccggctatg cgcaggctac ctgcggtcgg cgaatcgatg gtggctagca
     1261 tgttcagcag ggtcgtcttg ccgctgccgg agggacccat cacgcctagg aactcgccgg
     1321 ggaggatgcg caggtcgaag ccgtc
//
LOCUS       DAOQYE010000738         1342 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_127689_length_1342_cov_2.609946, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000738 DAOQYE010000000
VERSION     DAOQYE010000738.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1342
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127689_length_1342_cov_2.609946"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="PKN52_10675"
     CDS             <1..379
                     /locus_tag="PKN52_10675"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HNR00459.1"
                     /translation="RRARVLNILLLDEGTAQALGVDVGRVRLTLACLAGVATGTVVGY
                     AGSIAFVGLIVPFAVRLLIGDDHRSLLPAAIVGGAAVVTVGDAVARNLIAPMELPLGV
                     LMTVVGGPLFFWLIGRGRGVGRW"
     gene            complement(345..1067)
                     /locus_tag="PKN52_10680"
     CDS             complement(345..1067)
                     /locus_tag="PKN52_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNR00460.1"
                     /translation="MTSAKVLIVEDEVALAAVLKDNLEAEGFDVRHAPDGRAGATAWR
                     DERPDLVVLDVMLPHKDGYTLCRERRKAGDDTPVLFLSAKGSPDERVAGLEAGGDDYL
                     AKPFHLPEFLLRVRKLLDRSGALRAANARLVFGDNTVDLLSWTARTGDGHEFLLGERE
                     IGILRLLAGRSGEVVSRDEILDAVWGKGAFPSSRTVDNFVVRLRRHFEPDPAQPIYFH
                     TVWGVGYRFTPLTSGQPRARGR"
BASE COUNT          171 a          471 c          486 g          214 t
ORIGIN      
        1 gcgtagggcc cgggtcctca acatcctcct gctggacgag ggaacggccc aggctcttgg
       61 cgtggatgtc ggccgggtcc gactcacgct cgcctgtctg gccggcgtgg cgacgggcac
      121 tgtggtcggc tacgcgggca gcatcgcgtt cgtcgggctg atcgtgccgt tcgcggtgcg
      181 cctgctcatc ggggacgacc accgctcgct cctgccggcg gccatcgtcg ggggcgccgc
      241 cgtcgtcacg gtcggcgacg cggtcgcgcg caacctgatc gctcccatgg agttgccgct
      301 cggcgtgctc atgacggtgg tcggcggccc gctcttcttc tggctcatcg gccgcgggcg
      361 cggggttggc cgctggtgag cggcgtgaag cggtaaccga caccccagac cgtatggaag
      421 tagatgggct gagcggggtc gggctcgaag tggcgcctga ggcgcaccac gaagttgtct
      481 accgtgcggc tgctcgggaa cgcgcccttg ccccagacgg cgtcgaggat ctcgtcgcgg
      541 ctgactacct cgccggagcg gccggcgagc agtcgcagga tgccgatctc gcgctcgccg
      601 agcaggaact cgtggccgtc gcccgtcctg gccgtccagg acagcagatc aacggtgttg
      661 tcgccgaaca cgaggcgcgc gttcgccgcg cgtagcgcgc cggagcggtc gaggagcttg
      721 cgcacccgca gcaggaactc gggcaggtgg aagggtttgg ctaggtaatc gtcgccgccg
      781 gcctcgaggc cggcgacgcg ttcgtctgga ctccctttgg ccgacaggaa aaggacgggc
      841 gtgtcgtcgc ccgccttgcg ccgttcgcgg cagagcgtgt acccgtcctt gtgcggcagc
      901 atgacgtcga ggacgaccag gtcggggcgc tcgtcgcgcc acgccgtcgc acccgctcga
      961 ccgtcgggag cgtggcgaac atcgaacccc tcggcctcga ggttgtcctt caggaccgct
     1021 gcaagggcga cctcgtcttc gactatcagc accttcgcgc tagtcatcac aagctccacc
     1081 gtctggctgg gccgggcgcg cggcttcagc cgggcgcgcc accttcagcc gaccgccccg
     1141 cgcctggtgc aggcggcgct tcggcgcagg gggcgcccat accaggcgcg cctagctccg
     1201 gcggcgcgcc gcggccggcg gcgcctacct ggcctcctcg cgccggtcgt gggccaacgg
     1261 caattccggg gaaagcgccg ttatctcggc ccgcatcgcc tcgctcatct tgaactcggc
     1321 cgccgccagg gagggctcca gg
//
LOCUS       DAOQYE010000739         1341 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_127887_length_1341_cov_2.492224, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000739 DAOQYE010000000
VERSION     DAOQYE010000739.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127887_length_1341_cov_2.492224"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..844)
                     /locus_tag="PKN52_10685"
     CDS             complement(<1..844)
                     /locus_tag="PKN52_10685"
                     /inference="COORDINATES: protein motif:HMM:NF010539.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNR00461.1"
                     /translation="MFFRLGKEMGVDLGTATVLIYVKGQGIMLREPSVIAMVRDTGEV
                     KAVGEEAYRMLGRTPGNIVAVRPMRDGVIADYDLTEKMLQAFVRKVLTGPSRLFKPQI
                     MVCVPSGVTEVERRAVLQATREVGARKAFLIEEPLAAAIGAGVNIAEPTGSMIVDVGG
                     GTTDIAVISLGGIVVSESLRIAGNEFDESIIRHIRHKENLLIGDRTAEAVKMEVGAAM
                     LRGPGDDLSLEVRGRDLINGLPKSITVTTRDVVEALKEPIAKIADGVRRVLEQAPPAL
                     VSDVI"
     gene            complement(990..>1341)
                     /locus_tag="PKN52_10690"
     CDS             complement(990..>1341)
                     /locus_tag="PKN52_10690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00462.1"
                     /translation="PCVGASSLTALPGTLAPPATRVVALSDARRGNVYAQSLERLVAP
                     PWAVSYAESEPPRRLARADLDAEYPGMVRVEYGTTHQTGDTATLVVPDASALAWAADR
                     HAGAQAQPPKPHYL"
BASE COUNT          186 a          467 c          455 g          233 t
ORIGIN      
        1 cgatcacgtc gctgacgagt gccggcggcg cctgttcgag gacgcgtctt acgccgtccg
       61 cgatcttggc gataggctcc ttcagggcct cgacgacgtc cctggtggtg acggtgatgc
      121 tcttcggcag gccgttgatg aggtcgcgcc cgcggacctc gagactcagg tcgtcgcccg
      181 gcccgcgcag catggcggcc ccgacctcca tcttcacggc ctcggccgtg cggtcgccga
      241 tcagcaggtt ctccttgtgg cggatgtgcc tgatgatcga ctcgtcgaac tcgttgcctg
      301 cgatgcgtag gctttcggac acgacgatgc cgccgagcga gatgacggcg atgtccgtcg
      361 tgccgccgcc gacgtcgacg atcatcgagc ccgtcggctc ggcgatgttg acgcccgcgc
      421 cgatggcggc cgccagcggc tcctcgatga ggaacgcctt gcgggcgccc acctcgcggg
      481 tggcctgcag cacggcgcgg cgctccacct ccgtgacgcc ggagggtacg cacaccatga
      541 tctgcggctt gaagaggcgg ctcgggcccg tgagcacctt gcgcacgaac gcctgcagca
      601 tcttctctgt caggtcgtaa tccgctatga cgccgtcgcg catcgggcgg acggccacga
      661 tgttgcctgg cgtcctgcca agcatccggt aggcttcctc gcctacggcc ttcacctcac
      721 cggtgtcgcg gaccatcgcg atgacggacg gttcgcgcaa catgatgcct tggcccttca
      781 catagatgag gacggtcgcc gtgccgaggt cgacccccat ctcttttccg agtctgaaga
      841 acatactcac ttccgctcca cggcgttccc ggctaccacg acggtcaacg ggcctcaccg
      901 ccactcgggc gtggactcaa cgcccaccgg gccatccttg cggtgcggtc cgcgaggtca
      961 ccgtagtctc gcggtgaggc agtataacgc tacaagtagt ggggtttggg cggttgcgcc
     1021 tgcgcgccgg catgcctgtc agccgcccac gcgagcgcgc tggcgtccgg gaccacgagc
     1081 gtggcggtgt cgccggtctg gtgggtcgta ccgtactcga cgcggaccat gccggggtac
     1141 tcggcgtcga gatcggcgcg ggccagtctc cttggaggct ccgactccgc gtagctcaca
     1201 gcccacggcg gcgcgaccag gcgctcgagc gactgcgcgt agacgttgcc ccggcgcgcg
     1261 tcgctgagcg cgaccacgcg cgtggcgggt ggcgccagcg tacccggcag ggcggtgagg
     1321 ctggatgccc ctacgcacgg c
//
LOCUS       DAOQYE010000740         1340 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_128075_length_1340_cov_2.063813, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000740 DAOQYE010000000
VERSION     DAOQYE010000740.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1340
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128075_length_1340_cov_2.063813"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="PKN52_10695"
     CDS             complement(<1..383)
                     /locus_tag="PKN52_10695"
                     /inference="COORDINATES: protein motif:HMM:NF024313.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00463.1"
                     /translation="MSDGGLPGAAAGAAVVNAATGGSAPDTGAPAAFSRGVLGRLLRH
                     RSGSLGLVLTVAFAALAVVGPWVAPYDPAIPDYTHLAKGASGARTRRCRASGRDTFSR
                     ILAGARYSITIGLAATVIGALVGGA"
     gene            complement(376..1296)
                     /locus_tag="PKN52_10700"
     CDS             complement(376..1296)
                     /locus_tag="PKN52_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNR00464.1"
                     /translation="MLLYTLRRLLQAVPTLVGVVVLIFFFVRALPGDPARLYAGTDAD
                     RATVEAIRQRFGLDRSLPEQFAYFVGGLARGDLGRSFRANMPVKDLLARHFRATLVLA
                     SIAIVAATLLGIMLGVAAARRRNSALDLGITSASVLGISTPSFFLGILLIYVFAVKLR
                     VLPVAGSVSLKGLVLPAATLAVASLGTIARFARSSLLETIGNDYVRTARAKGLEQRRV
                     INKHALRNALIPVVTIVGLQFGFLLSGTVIVETVFNFHGLGWLLIQSVEARDYPAVQG
                     LMLVFALQFIVINLIVDVLYGLIDPRISYE"
BASE COUNT          222 a          452 c          508 g          158 t
ORIGIN      
        1 caggcgccgc ctacgagggc cccgatgacc gtggccgcca gcccgatcgt gatcgagtag
       61 cgcgccccgg cgaggatgcg gctgaacgtg tcgcgcccac tggctcggca ccgacgcgtt
      121 cgggcgccgc tcgcgccctt ggcgagatgg gtgtagtcgg ggatggccgg gtcgtacggc
      181 gccacccacg gcccgacgac cgccagcgcc gcgaacgcga ccgtgagcac gagcccgagc
      241 gacccgctcc ggtggcgcag cagccggccg agcacaccgc gggagaaggc ggcgggcgcg
      301 cctgtgtcgg gtgcagagcc gccagtcgcg gcgttgacga cagccgcgcc ggccgcggcg
      361 cccgggaggc cgccgtcact catagctgat ccgcgggtcg atgagcccgt agagcacgtc
      421 cacgatgagg ttgatgacga tgaactgcag cgcgaacacg agcatcaggc cctgcaccgc
      481 cgggtagtcg cgcgcctcca ccgactggat gagcagccag ccgagcccgt ggaagttgaa
      541 gacggtctcg acgatgaccg tgccggagag gaggaacccg aactggagcc cgacgatggt
      601 gacgacgggg atgagggcgt tgcgcagcgc gtgcttgttg atgacccgcc gctgctcgag
      661 ccccttggcg cgggcggtgc ggacgtagtc gttgccgatc gtctccagga ggctggagcg
      721 cgcgaaacgc gcgatggtgc cgaggctggc cacggccagc gtcgcggcgg gcaggacgag
      781 gcccttgagg ctgacgctgc ccgccaccgg cagcactcgc agcttcacgg cgaagacgta
      841 gatgaggagg atgccgagga agaacgacgg ggtgcttatg cccagcacgg acgcggacgt
      901 gatgccgagg tcgagcgccg agttcctgcg gcgcgcggcg gccacgccga gcatgatgcc
      961 gagcagcgtg gccgccacga tggcgatgct ggcgagcacg agcgtcgcgc ggaagtgccg
     1021 cgccagcagg tccttcacgg gcatgttcgc gcggaacgac ctgccgaggt cgccccgcgc
     1081 gaggccgccg acgaagtacg cgaactgctc cgggagcgac cggtcgaggc cgaagcgttg
     1141 ccggatggcc tcgacggtcg ccctgtcggc gtcggtcccc gcgtacagcc gcgccgggtc
     1201 gcccggcagc gcccgcacga agaagaagat gagcaccacc acgccgacga gcgtcggcac
     1261 ggcctggagc agccgcctga gggtgtagag cagcatcgcg gctaccccgg cctaggggcg
     1321 gttaccgctt caacgcagct
//
LOCUS       DAOQYE010000741         1339 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_128180_length_1339_cov_2.454050, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000741 DAOQYE010000000
VERSION     DAOQYE010000741.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1339
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128180_length_1339_cov_2.454050"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(83..1267)
                     /locus_tag="PKN52_10705"
     CDS             complement(83..1267)
                     /locus_tag="PKN52_10705"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HNR00465.1"
                     /translation="MDPAADSAATIAATWRPGVIDLGPGHPSTDLLPLELLRSAAWGA
                     LADDPSILQYGAEQGSERFRRQLAAVLETETGVATDPDALFVTGGASQALDILCTLYT
                     RPGQTVLVAEPTYFLALRVFADRGLNVVPVPCDEHGPLPEALEAALAAHRPALFYLVP
                     IFGNPTGVTLTAERRAAVVELTAGTGTLVVADEVYRLLQFEGRSAASLAGEGREHVVS
                     LNSFSKTLGPGLRLGWFAGSEAVIGRVRASGFLRSGGGLNPLVAILVGELLADGRFSD
                     HVRNLRRAYAQRAGALAAALREHVPELAFDEPRGGYFIWARLPGVGAERLRGPAREAG
                     ADFAPGAAFSAGGGFSDHLRLSFSRYAPEELAEGARRLGAAVDAAVNAAVRTATRAAQ
                     AL"
BASE COUNT          206 a          480 c          483 g          170 t
ORIGIN      
        1 tggcgggtcc gcttggccat gagctaactc ggatgttacg cgcggcgggc tagggccggc
       61 tgtcctcgaa cggcacgatc cctcatagcg cctgcgccgc gcgcgtggcc gtgcgcacgg
      121 ccgcgttcac ggccgcgtca acggccgcgc ccaggcggcg cgcgccctcg gctagctcct
      181 ccggcgcgta acgactgaac gacaggcgca ggtgatccga gaacccacca ccggccgaga
      241 acgccgctcc gggcgcgaag tcggcgcccg cctcgcgcgc tgggccacgc aagcgctccg
      301 cacccacgcc cggtagccgg gcccagatga agtaaccgcc ccgcggctcg tcgaaggcga
      361 gctcgggaac gtgctcgcgc agcgccgccg cgagcgcgcc agcgcgctgc gcgtacgctc
      421 ggcgcaggtt gcgcacgtgg tcggagaacc gtccgtcggc aagcagctcg cccaccagga
      481 tcgcgacgag ggggttgagc ccgccgccgc tcctgaggaa gccgctcgcg cgtacgcggc
      541 cgatgaccgc ttcggaaccg gcgaaccaac ccagccgcag gccggggccg agcgtcttgg
      601 agaacgagtt gagcgacacg acgtgctcgc gtccctcccc agccaggctc gcggccgatc
      661 ggccctcgaa ctggaggagg cggtagacct cgtcggccac gacgagcgtt cctgtgccgg
      721 cggtgagctc gacgacggcc gcgcgccgct cggccgttag cgtcacgccc gtggggttgc
      781 cgaagatggg cacgaggtag aagagcgccg gccgatgcgc cgcgagcgcg gcctcgagcg
      841 cctccggcag cgggccgtgc tcgtcgcatg gcacgggcac gacgttgagg ccacggtcgg
      901 cgaacaccct cagagcgagg aagtaggtcg gttcggccac cagcaccgtc tgaccgggcc
      961 gcgtgtacaa ggtgcagagg atgtcgagcg cctgcgaggc gccaccggtg acgaacagcg
     1021 cgtccgggtc ggtggcgacg cccgtctcag tctccaacac ggccgcgagc tgccgcctga
     1081 accgctcgga accctgctcg gcaccgtact ggaggatgga ggggtcgtcg gctaaggcac
     1141 cccacgccgc cgagcgcagc agttccaggg gcagcaggtc ggtcgacggg tgtcctgggc
     1201 cgaggtcgat gaccccgggc cgccacgtgg cagctatcgt cgcggcgctg tcggcggctg
     1261 ggtccatggg acccacgata ttactggagc gtcattactg gagcgtcatt acgccccagt
     1321 tactcgcgcc agatcgccc
//
LOCUS       DAOQYE010000742         1338 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_128362_length_1338_cov_2.169914, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000742 DAOQYE010000000
VERSION     DAOQYE010000742.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1338
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128362_length_1338_cov_2.169914"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..895)
                     /locus_tag="PKN52_10710"
     CDS             complement(<1..895)
                     /locus_tag="PKN52_10710"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNR00466.1"
                     /translation="MTSRAYRYVIVGGGMTGDAAAKAIRKADPDGSLLLIGDETVAPY
                     KRPPLSKGLWKGDPEAKIDLGTAARGAAVLTGTKVTGIDLDERTVTLADGAAVGYERL
                     LLATGARARQLPDLPSGGPVVAFRRLGDYHAAKARSGPGKVAAIVGGGFIGAELAAAL
                     RNAGGEVHMIYPEAEPGANRFPPELAALVGENYRARGVILHPGTHVASANVADDQATL
                     TLADGTSLRADLVVVGIGAVPNDEIAKAAGLKVDNGVWVDGHLRAARAEGAGGDEGHA
                     ADAARQGAEEGAESSPDTGRAT"
     gene            1114..>1338
                     /locus_tag="PKN52_10715"
     CDS             1114..>1338
                     /locus_tag="PKN52_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00467.1"
                     /translation="MSSAHHGPATELTVFEDRAQAPRDLRTRAELSERGFTRLGEPAG
                     LLRGAAGLEPLYSTSEARRLKGDTWPRAVRQ"
BASE COUNT          165 a          496 c          493 g          184 t
ORIGIN      
        1 gcgttgcgcg ccccgtgtcg gggctcgact cggcgccctc ctcggcacct tggcgcgcag
       61 cgtcggcggc atgaccctcg tcgccgccgg cgccctcggc ccgcgcggcc ctgagatgcc
      121 cgtccaccca gacgccgttg tcgaccttga gcccggccgc cttggcgatc tcgtcgttgg
      181 gcacggcgcc gatcccgacc acgacgaggt cggcgcgcag cgaggtgccg tcggcgaggg
      241 tgagggtcgc ctggtcgtcg gccacgttgg ccgacgcgac gtgcgtgccc gggtggagga
      301 tcacgccgcg ggcgcggtag ttctcgccga cgagcgcggc gagttctggc gggaagcggt
      361 tggcgcccgg ctcggcctcc gggtagatca tgtggacctc gccacccgcg ttcctgaggg
      421 cggccgccag ctcggcgccg atgaacccgc cgccgacgat cgcggctacc ttccctgggc
      481 cggagcgcgc cttggccgcg tggtagtcgc ccaggcggcg gaaggcaact acaggtccgc
      541 cgctaggcag gtcggggagc tggcgcgccc tggcgcccgt ggcgagcagg aggcgctcgt
      601 agccgaccgc cgcgccgtcc gcgagcgtga cggtgcgctc gtcgaggtcg atgcccgtca
      661 ccttcgtgcc ggtcaggacg gctgcgccgc gcgccgccgt accgaggtcg atcttcgcct
      721 cggggtcgcc cttccacagg cccttggaca acggcgggcg cttgtagggc gccacggtct
      781 cgtcgccgat gagcaacagc gagccgtcgg ggtcggcctt ccggatcgcc ttggccgcgg
      841 cgtcccccgt catccccccg cctacgatca cgtaccgata agcacggctt gtcacggtct
      901 caccttcacc acgacgctac cacgcaaacg acacttttcc aagcgtttac ttgtgaaagt
      961 cgggcgggtc cggaggccgg tgtacggccg tgggccgtcg tgcggggcgg cgccaagtgg
     1021 cgcgccgtcg ggcaaacgcg caccggacga gagcggggcg accgtgcagc cccgctgccc
     1081 ggcgaccatc acccgcttcg gtacactgac ggcgtgagta gcgcgcacca cggcccggcg
     1141 acggagctga cggtcttcga ggaccgcgcg caagcgccac gcgacctgcg cacccgggcc
     1201 gagctgtccg agcggggctt cacgcggctc ggcgagcccg ccgggctgct gcgcggcgcg
     1261 gccggcctgg agcccctgta cagcacgagc gaggcgcggc gcctcaaggg cgacacctgg
     1321 ccgcgagccg tgcgccag
//
LOCUS       DAOQYE010000743         1338 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_128384_length_1338_cov_1.957132, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000743 DAOQYE010000000
VERSION     DAOQYE010000743.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1338
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128384_length_1338_cov_1.957132"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1338
                     /locus_tag="PKN52_10720"
     CDS             <1..>1338
                     /locus_tag="PKN52_10720"
                     /inference="COORDINATES: protein motif:HMM:NF017009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HNR00468.1"
                     /translation="ADRLLDGPGDPLLRQHAEDLRAIPFAVDQDRLRVAFVDPLDALA
                     IEEVEDASGCLVEPYLALQKEMLWALATYYPELGLEPPVDIEVDPAMRLGNLAVGGGF
                     LTEKQLTEAIEEQQRTGGLLGRILVARGHLSEEQLTALLAEQHGIPFVPDLTGEPTEE
                     RLATKLLRLDAVQFSAVPYKFDGDKLVVAISDPRRMADIESIVTGDVLFVAATGPAVS
                     ARIDEMYAQDQGRLGETLLQSKRLKREQLRRALEEQAKLGRIKPLGEILVDLGYVTQT
                     DVEEALNKQRSGGGRLEDTLVQSGKISPDMLARSLAMQLGFEFIEDNARIDPYAVSLV
                     PEPTARRYAAMPVRLEGDVLVVAMKDPRHVFALDDIHLITGREIRPAVATEESLTRML
                     NRFYREGADMEELAKAVVEEVGVSSTSEEDDTSAIDDNALVKVVNNIIRESVLN"
BASE COUNT          232 a          474 c          442 g          190 t
ORIGIN      
        1 gccgatcgac tcctcgacgg ccctggcgat ccgctgctcc gacagcacgc cgaggacctg
       61 cgcgccatcc cgttcgccgt cgaccaggac cgcctgcgcg tggcgttcgt cgacccactg
      121 gacgccctgg cgatcgagga ggtcgaggac gcctccggct gcctggtgga gccgtacctc
      181 gccctgcaga aggagatgct gtgggcgctg gcaacctact accctgagct cgggctcgag
      241 cccccggtcg acatcgaggt cgaccctgcc atgcggctcg gcaacctggc cgtcggcggc
      301 ggcttcctca cggagaagca gctgaccgag gccatcgagg agcaacaacg cacgggcggg
      361 ctcctggggc gcatcctggt cgctagaggc cacctgagcg aggagcagct gacggcgctc
      421 ctggccgagc agcacggcat accgttcgta cctgacctca ccggcgagcc gacggaagag
      481 cgcctggcca ccaagctcct ccgcctcgac gccgtgcagt tctccgccgt gccttacaag
      541 ttcgacggcg acaagctcgt cgtggccatc tcggacccgc ggcgcatggc cgatatcgag
      601 tccatcgtga ccggcgacgt cctgttcgtg gccgccaccg gcccagccgt ctccgcgcgc
      661 atcgatgaga tgtacgccca agaccagggg cgcctcggcg agacgctgct gcagagcaag
      721 cgcctcaagc gcgagcaact gcgtagggcc ttggaggagc aggcgaagct gggccggatc
      781 aagccgttgg gcgagatcct ggtcgacctc ggctacgtca cgcagaccga cgtcgaggag
      841 gcgctcaaca agcagcgctc cggcggcggt cggctcgagg acacgctagt gcagtccggc
      901 aagatcagcc cggacatgct ggcgcgcagc ctcgccatgc agctcggctt cgagttcata
      961 gaggacaacg cgcgcatcga tccgtacgcc gtcagcctcg tcccggagcc gacggccagg
     1021 cgctacgccg ccatgccggt gcgcctcgag ggcgacgtcc tcgtcgtggc gatgaaggac
     1081 ccgcgccacg tgttcgcgct cgacgacatc cacctgatca cgggccgcga gatccgacct
     1141 gccgtggcga cggaagagtc gctcacgcgc atgttgaacc gcttctaccg cgagggcgcc
     1201 gacatggagg agctcgcgaa ggcggtcgtc gaggaggtcg gggtctcctc cacctccgag
     1261 gaggacgaca cgagcgccat cgacgacaac gcgctcgtca aggtcgtcaa caacatcatc
     1321 cgcgagtccg tcctcaac
//
LOCUS       DAOQYE010000744         1336 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_128633_length_1336_cov_3.138954, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000744 DAOQYE010000000
VERSION     DAOQYE010000744.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1336
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128633_length_1336_cov_3.138954"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            15..>1336
                     /locus_tag="PKN52_10725"
     CDS             15..>1336
                     /locus_tag="PKN52_10725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00469.1"
                     /translation="MQSDETNPWLYLEAARAATPGSTEEARYVDGALRNAVTFYERAQ
                     LARELMRFSPQKREEATTAMYSALEDFVARGYRAELLFDQDLAEAYGFPLGAMTTALG
                     RGDMQSAGFWADWVYFLATPAVPQTQAALADYAAYLRADGQREASNLWRERSREGAGF
                     DLAASVRKVALDVGRTGWYGVVAMLVALLALYIALAAKYWRPQTLNLRRRSAKAGGGA
                     RLFFMRYATFTERLVAVLLLAAALALAALNGWAREGDSLPRAWGSGTLASVPALDALA
                     RLGGMSQATAFVRGYAEQSADAVDPAEVAYRAAGDDADAANNLGALLGDASLYQRALE
                     LKPGLPAASFNVGRGANPSRLMATFAPDAPALVVPDETRLRSAVAGTFQTALTSMFTN
                     PWAGLVGLAGVTLPQWAWLIVVVAFLLLVLCNVVMLVMPRPREARNAPR"
BASE COUNT          180 a          482 c          467 g          207 t
ORIGIN      
        1 acccgcagga acgcttgcag agcgacgaga cgaacccttg gctctacctc gaggccgccc
       61 gcgcggccac gccgggcagc acggaagagg cgcgttacgt tgacggggcg cttcggaacg
      121 ccgtcacgtt ctacgagcgc gcgcaactgg cgcgcgagct catgcggttc tcgccgcaga
      181 agcgcgaaga ggcgaccacg gccatgtact cggcgctcga agacttcgtg gcgcgcggtt
      241 atcgggcgga gctcctcttc gatcaggacc tcgcggaggc gtacggcttc cccctgggcg
      301 ccatgacgac ggccctcggc aggggcgaca tgcagtccgc gggcttctgg gccgattggg
      361 tctacttcct cgccaccccc gccgtccctc agacgcaggc cgccctggcg gattacgccg
      421 cgtacctccg agccgacggc cagcgcgagg cctccaacct ctggcgcgag cggtcccgcg
      481 aaggggccgg cttcgacttg gccgcctccg tacgcaaggt cgcactggac gtcggcagga
      541 ccggttggta cggggtcgtc gccatgctcg tcgcgctgct ggccctctac atcgccttag
      601 cggcgaagta ctggcgtccg cagacgctca acctccgcag gcgttcggcc aaggccggcg
      661 gaggagcccg gctcttcttc atgcgttacg cgacgttcac ggagaggctc gtggccgtgc
      721 tgctgctggc ggccgcgctg gcgctcgccg cgctgaacgg ctgggcgcgg gagggcgatt
      781 cgctgccgag ggcgtggggg tcgggcacgc tcgcctccgt gccggcgctg gatgcgctag
      841 cgcgcttggg aggcatgagt caggccactg cgttcgtgcg cggctacgcc gagcagagcg
      901 cagacgctgt cgatcccgcc gaggtcgcgt atcgcgcggc cggcgatgac gccgacgccg
      961 ccaacaacct tggcgcgctg ctgggcgacg cgtcgctcta ccaacgcgcc ctcgagctca
     1021 aaccggggct gccggcagcc agcttcaacg tcggtcgcgg agcgaacccg tcacgcctca
     1081 tggccacgtt cgcgccggac gcgccggcgc tcgtcgttcc cgacgagacc cgcctgcgca
     1141 gcgccgtggc cggtaccttc cagaccgcgc tgacgagcat gttcaccaac ccgtgggcgg
     1201 gtcttgtcgg actcgccggc gtcacgctgc cgcagtgggc gtggctgatc gtcgtggtcg
     1261 cgttcctcct cctcgtgctc tgcaacgtcg tgatgctggt catgccccgg ccgcgcgagg
     1321 cacgcaacgc cccacg
//
LOCUS       DAOQYE010000745         1335 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_128802_length_1335_cov_2.717969, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000745 DAOQYE010000000
VERSION     DAOQYE010000745.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1335
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128802_length_1335_cov_2.717969"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1335)
                     /locus_tag="PKN52_10730"
     CDS             complement(<1..>1335)
                     /locus_tag="PKN52_10730"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HNR00470.1"
                     /translation="TIASSTSDGSVRGRFVPYVLGAGLLALALGAAAFTGYALLLHER
                     CLGFAATAVLGLVAGWVLRSRGRSGAEPSRREALVAVLTSWLLLPALAAAPYVIDGGM
                     SALNAIFESMSGFTTTGATVLTDFSAFGKALFMWRALSQWVGGIGILVLFIAVFPQLA
                     IAGRQLFFTEAPGPTEERLTPRLRNTAVAVLGVYSALTLACAVGYGVAGMGSYDAIAH
                     ALTTLAAGGFSPNGLSFAGFENSAMEWVAILFMFFAGANFALLYRAATGRPRDLLRDA
                     ELRTYAAILLAASGVLTLLLVDSYPPLDAVRHSLFQVASILTSTGFASADFALWSLPA
                     QMVLVVLMFVGGCAGSASGGVKVVRWIVIAKSTARELIRALHPRAVLPVRVGARTVSE
                     EVVRAVAAFLTLYIGLFAVTTLILVVLGADFVVAFTAAISCLGNVGPGLESIG"
BASE COUNT          255 a          446 c          467 g          167 t
ORIGIN      
        1 ccgatgctct ccaagccagg ccccacgttg ccaaggcagg agatcgcggc cgtgaacgcc
       61 accacgaagt cggcccctag cacgaccagg atcagggtcg taaccgcgaa cagcccgata
      121 tagagggtga ggaacgccgc aacggcgcgc accacctcct ctgacacggt gcgtgcgcct
      181 accctcacag ggagcacggc gcgcgggtgg agggcgcgga tcaactcgcg tgccgtgctc
      241 ttggcgatga cgatccagcg aacgaccttc acgccaccac tggccgagcc ggcgcagccg
      301 cccacgaaca tcaggaccac gagcaccatc tgggcaggca gagaccacag tgcgaaatcg
      361 gcggaggcga agcccgtgct ggtcaggatg ctcgccacct ggaacaagga gtgtcggacc
      421 gcgtccaagg gcgggtaact gtccaccagt agcagcgtga gcacgccgct cgccgccagg
      481 agtatggccg cgtaggtccg gagctcggca tcgcgaagga ggtcgcgcgg ccgtccggtg
      541 gcggcgcggt agagcagcgc gaagttggcg ccggcgaaga acatgaacag gatggcgacc
      601 cattccatgg cgctgttctc gaacccggcg aagctgagcc cgtttgggct gaagcctccc
      661 gcggccagcg tggtgagtgc gtgggcgatg gcgtcgtagg agcccatccc tgccactccg
      721 taaccgaccg cgcaggcgag cgtgagtgcc gagtagacgc caaggaccgc taccgccgtg
      781 ttccttagcc gtggggtgag gcgctcctcg gtcggcccgg gcgcctcggt gaagaagagc
      841 tgccgcccgg cgatggccag ttgcgggaaa acggcgatga acagcaccag gatgccgatg
      901 ccgcccaccc attgcgacaa ggcgcgccac atgaagagcg ccttcccgaa cgcggagaag
      961 tccgtcagga ccgtggctcc cgtggtagtg aacccgctca tggactcgaa gatggcgttc
     1021 agtgcggaca tgccgccatc gatgacgtac ggtgcggcgg cgagcgccgg cagcaggagc
     1081 caggacgtca gtacggctac gagcgcttcg cggcggctcg gctccgcacc gctacggccc
     1141 cggcttcgta atacccaccc ggccacgagc cccaggacgg cggtggccgc gaagcctaga
     1201 cagcgctcgt gcagcagcag cgcgtagccg gtgaaagccg cggcgcctag ggctagggcg
     1261 agcaggcccg ccccgaggac ataggggacg aaccgccccc ggacgctgcc gtcggacgtg
     1321 ctcgaggcga tcgtc
//
LOCUS       DAOQYE010000746         1334 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_128970_length_1334_cov_2.937451, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000746 DAOQYE010000000
VERSION     DAOQYE010000746.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128970_length_1334_cov_2.937451"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..880
                     /locus_tag="PKN52_10735"
     CDS             <1..880
                     /locus_tag="PKN52_10735"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNR00471.1"
                     /translation="TSDHPLGAVRTGVRVRLGYYDQGLKGVDGDATLFEELLKRMGDA
                     DAHNALGRFLFPYEAQFKRVRDLSGGERARLALLDLTLARRNLLVLDEPTNHLDVEMI
                     EALEAALDAYEGTLILVSHDRRFLSRLATRVWEVRDGRFTDYAGDWDYYVRKRREGTD
                     GGPPAAATSGAGRKPGTPAQRAVAIAERKAASRWQLERRLAVLEGDIAQVEGRLADVT
                     RLLGSPMALAVGDLAALGVGDWSVPGPPPSSAELLAALGAEHAILEERLLQHMEEWED
                     VTDQLSDFAATSGKGN"
     gene            901..>1334
                     /locus_tag="PKN52_10740"
     CDS             901..>1334
                     /locus_tag="PKN52_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HNR00472.1"
                     /translation="MQRVRYRSFDGIHWGELEGGMIHQLTHMLGSPSGHRVPLSDVSL
                     LPPCEPNVIVCVGRNYADHIRELGNDKSGLPTEPGLFLKGLNTLSGAGDDVPYPAWTD
                     DLQYEGELAVVIARELRNVVVEEALDHVLGYTCALDVTARDK"
BASE COUNT          221 a          475 c          454 g          184 t
ORIGIN      
        1 cacgtccgac cacccacttg gcgccgtacg cacgggcgta cgggtacgcc tcgggtacta
       61 cgaccagggc ctcaaggggg tcgacggcga cgccaccctc ttcgaggagc tcctcaagcg
      121 catgggcgac gccgacgcgc acaacgccct cggccgcttc ctcttcccct acgaggcgca
      181 gttcaagcgc gtcagggacc tttccggtgg cgaacgggcg cgcctggcgc tcctcgacct
      241 caccctggcg cgccgcaacc tcctcgtgct cgacgagcca actaaccacc tcgacgtcga
      301 gatgatcgag gcgctggagg ccgcgctcga cgcctacgaa ggcaccctca tcctcgtctc
      361 gcacgacagg cgcttcctct ccaggctcgc cacgcgcgtc tgggaggtga gggacgggcg
      421 cttcacggac tacgcgggcg actgggacta ctacgtgcgc aagcgtcggg aagggaccga
      481 cggcggacca ccagcggctg cgaccagcgg cgcggggcgg aagcccggca cgcccgccca
      541 gcgagcggtg gccatcgcgg agcgcaaggc cgcgtcccgc tggcagttgg agcggcggct
      601 cgcggtgcta gagggcgaca tcgcgcaggt cgaggggcgg cttgccgacg taacgcggct
      661 gctcggctcg cccatggccc tcgcggtcgg cgacctcgcg gcgctcggcg tcggcgactg
      721 gagcgttccc gggcccccgc cctcgagcgc cgagctcctg gccgccctgg gcgccgagca
      781 tgccatcctg gaagagcggc tcctccaaca catggaggag tgggaagacg tgaccgatca
      841 gctgagcgac ttcgccgcca ccagcggcaa gggtaactag ttcgaggagg cgaggaccag
      901 atgcaacgcg ttcgttaccg gagcttcgat gggatccatt ggggcgagct agagggcggc
      961 atgatccacc agttgacgca catgctcggc tcaccgagcg ggcaccgtgt gcccttgagc
     1021 gacgtctcgc tcctaccgcc gtgcgagccg aacgtcatcg tgtgcgttgg caggaactac
     1081 gccgatcaca tccgcgagct cggcaacgac aagtcgggcc tgcccaccga gccggggctc
     1141 ttcctcaagg gcctcaacac cctgagcggc gctggcgacg acgtgcctta cccggcctgg
     1201 accgacgacc tgcagtacga gggcgaactg gccgtcgtca tcgccaggga actgcgcaac
     1261 gtcgtggtcg aggaggcgct cgaccacgtc ctcggctaca cgtgcgccct cgacgtgacg
     1321 gcgcgcgaca agca
//
LOCUS       DAOQYE010000747         1331 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_129461_length_1331_cov_3.996865, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000747 DAOQYE010000000
VERSION     DAOQYE010000747.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1331
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129461_length_1331_cov_3.996865"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="PKN52_10745"
     CDS             <1..403
                     /locus_tag="PKN52_10745"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNR00473.1"
                     /translation="GLRAVRRLGQEPELPFTTKQVLLRVAQEALHNVVKHASASTVTV
                     RAAVEAEAGSPGAPATEAEAPAGPTLRLEVVDDGVGFDTSLAYPGHLGLTSMQERVAA
                     LGGTLSIESAQGAGTSVTVIVPVSPPTGSEP"
     gene            427..1032
                     /locus_tag="PKN52_10750"
     CDS             427..1032
                     /locus_tag="PKN52_10750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00474.1"
                     /translation="MSTGTVLLLAGAALLLRQLGSHSLFFELVWLAAIVAGAAAAFRS
                     LAGRVPFRVRLIVHAAFGLFALFSLNRLAGAALFGFVALYFWLLFTSPRPAGQRPATW
                     QVLVAGVVGTLALLTGADALMPAGDNSVILFLGVTATFTAIYLLPKERGGARWALWPA
                     LAWAALTLLINDPSGGLARWLLPLALIGAGVAFLGYMRGRG"
     gene            complement(1106..>1331)
                     /locus_tag="PKN52_10755"
     CDS             complement(1106..>1331)
                     /locus_tag="PKN52_10755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00475.1"
                     /translation="ALLGRLPLEAGDLVYLSPFVLQPGSVYAARAAEGGVAPLSEAEA
                     AAQYRELLAGAKARVGTAKVALYHIDEFVY"
BASE COUNT          167 a          484 c          467 g          213 t
ORIGIN      
        1 cgggctgcgc gcggtgcgcc gacttgggca ggaaccggag ttgcctttca cgacgaaaca
       61 ggtcctcctg cgagtggcgc aggaggccct ccacaacgtg gtgaagcacg cctcggcgtc
      121 gacggtcacc gtgcgggccg cggtcgaggc cgaggccggt tcgcccggcg cgccagccac
      181 ggaagccgaa gcgcccgccg gaccgacctt gcgcctcgag gtcgtcgacg acggagtcgg
      241 gttcgacacc tctctcgcct atcccggtca tctcgggctg acgtcgatgc aagagcgcgt
      301 cgcggcgttg ggtggcacct tgagcataga gagcgctcaa ggagcaggca cgtccgtgac
      361 cgtgatcgtg cccgtctcgc cgccaaccgg gtctgagcca tgacgcctcc cggaccgacg
      421 cgcaaggtga gcacgggcac cgtgctgctc ctcgccgggg ccgctctgct gctgaggcag
      481 ctggggagcc atagcctctt cttcgagctg gtgtggctgg cggcgatcgt ggccggcgcg
      541 gccgcggcgt tccgctccct cgccggccgc gtgccgttcc gggtacgcct tatcgtccac
      601 gccgccttcg gcctcttcgc cctgttcagc ctgaaccggc tggcgggagc ggcgctgttc
      661 ggtttcgtcg ccctctactt ctggctcctg ttcacgtcgc cccgacccgc gggtcagcgc
      721 ccggcgacct ggcaggtgct cgtcgccggc gtggtcggca cactcgcgct gctcaccggc
      781 gcagacgcgc tcatgcccgc cggcgacaac agcgtcatcc tctttctcgg cgtcaccgcc
      841 accttcacag ccatctacct gctcccgaag gaacggggcg gcgcgcgctg ggcgctatgg
      901 cccgcgctgg cgtgggcggc gctgaccttg ctcatcaacg acccgagcgg cgggctggcg
      961 cgctggctgc tgccgctcgc cctgatcggg gcaggcgtcg ccttcctcgg gtacatgcgc
     1021 ggcagggggt gacggcgcca tttcgcgccg cggccggcct cgccacgggc cccgacgtca
     1081 ccgcccgcca ggctcggtat atgggtcagt acacgaactc gtctatgtga tagagcgcca
     1141 ctttggctgt accgacgcgc gccttggcgc cggcgagcag ttcgcggtac tgggctgcgg
     1201 cctccgcctc gctcagcggc gcgaccccgc cctcggccgc gcgagcggcg tagacggacc
     1261 cgggttgcaa gacgaacggt gacaggtaga cgaggtcgcc agcctcgagg ggtaggcgcc
     1321 ccaggagcgc g
//
LOCUS       DAOQYE010000748         1330 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_129693_length_1330_cov_2.370196, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000748 DAOQYE010000000
VERSION     DAOQYE010000748.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129693_length_1330_cov_2.370196"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            15..629
                     /locus_tag="PKN52_10760"
     CDS             15..629
                     /locus_tag="PKN52_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HNR00476.1"
                     /translation="MWAGGIFAVLMIVALPFYARVSNNGVGVPVAQPIDYPHNLHVVQ
                     VGLDCRYCHTTVETSNTASVPPTETCMGCHSQLRVGTPQVQAVVDSWNSGTPIAWNRV
                     NELADYVYFNHSAHVNNGVGCSTCHGRMDEMAQVWKNEPLTMGWCLECHRNPATALRP
                     VSEVFNMAYQPLADPKAQLALGATLVEAYHIDTDKLIQCSTCHR"
     gene            626..>1330
                     /locus_tag="PKN52_10765"
     CDS             626..>1330
                     /locus_tag="PKN52_10765"
                     /inference="COORDINATES: protein motif:HMM:TIGR04519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-variant-translocated molybdopterin
                     oxidoreductase"
                     /protein_id="HNR00477.1"
                     /translation="MSDAQIDFTDIRAKLAKHEGPDYWRSIDQLAETEEFKLFLQREF
                     PRQAAPLEGSFERRDFLKLLGASMALAGLSACARPPLAHEKIVPYVKQPEQITPGRPL
                     YFATAVTYAGYAEGVLAESHQGRPTKLEGNPDHPASLGATGAITQAQVLTLYDPDRAQ
                     EVLQKGQPSTWDDFTSALSGTLAGLPGGDGFALLTENVTSPTLASQISDFLGQHPRAR
                     WFQYDPVHPDNVVAGAR"
BASE COUNT          225 a          524 c          394 g          187 t
ORIGIN      
        1 tcgtcacctg ggcggtgtgg gcgggcggca tcttcgccgt gctcatgatc gtcgccctcc
       61 cgttctacgc gcgcgtctcc aacaacgggg tcggcgtgcc ggtagcccaa ccgatcgact
      121 acccgcacaa cctccacgtc gtccaggtcg gcctcgactg ccgctactgc cacaccacgg
      181 tcgagacctc caacacggcc agcgtgcccc cgaccgagac gtgcatgggc tgccactcgc
      241 agctgcgcgt cggcaccccg caggtccagg cggtcgtcga ctcctggaac tccggcacgc
      301 ccatcgcctg gaaccgcgtc aacgagctcg ccgattacgt ctacttcaac cacagcgccc
      361 acgtgaacaa cggcgtcggt tgcagcacgt gccacggccg catggacgag atggcccagg
      421 tctggaagaa cgagcctctg accatgggtt ggtgcctcga gtgccaccgc aacccggcga
      481 cggccctgcg gcccgtgagt gaggtcttca acatggcgta tcaaccgctc gccgacccca
      541 aggcgcaact ggccctgggc gccaccctcg tggaggcgta ccacatcgac accgacaagc
      601 tcattcaatg ctcgacgtgc caccgatgag cgacgcccag atcgacttca ccgacatccg
      661 cgccaagctc gccaagcacg agggccctga ctactggcgc tccatcgacc agctcgccga
      721 gacggaggag ttcaagctct tcttgcagcg cgagttcccg cgccaggcgg cccccctcga
      781 gggcagcttc gagcgccgcg acttcctgaa gctgctcggg gcgagcatgg cgctcgccgg
      841 cctcagcgcc tgcgcccggc cgccgctcgc gcacgagaag atcgtgccgt acgtcaagca
      901 gccggagcag atcacgcccg gccgccccct ctacttcgcg accgccgtca cctacgccgg
      961 ctacgccgag ggtgtgctcg ccgagagcca ccaggggcga cccaccaagc tagagggcaa
     1021 ccccgaccac ccggccagcc ttggcgccac cggcgccatc acccaggcgc aggtcctgac
     1081 cctctacgac cccgaccgcg cgcaggaggt cctccagaaa ggccagccga gcacgtggga
     1141 cgacttcacg tccgccctat cgggtaccct ggccggcttg cctggcggcg acgggttcgc
     1201 gctcctaacc gagaacgtca cctcgccgac gctggcctcc cagatctccg acttcctcgg
     1261 gcagcacccg cgggcccggt ggttccagta cgaccccgtc cacccggaca acgtcgtcgc
     1321 cggcgcccgt
//
LOCUS       DAOQYE010000749         1330 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_129716_length_1330_cov_2.127059, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000749 DAOQYE010000000
VERSION     DAOQYE010000749.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129716_length_1330_cov_2.127059"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="PKN52_10770"
     CDS             complement(<1..211)
                     /locus_tag="PKN52_10770"
                     /inference="COORDINATES: protein motif:HMM:NF012964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HNR00478.1"
                     /translation="MNRPPTGIVMLNLGGPRNLDEVKPFLMELFADREIIQLPAQSTL
                     GPFIAGRRTKSVQKNYSDIGGGSPLE"
     gene            complement(248..>1330)
                     /locus_tag="PKN52_10775"
     CDS             complement(248..>1330)
                     /locus_tag="PKN52_10775"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNR00479.1"
                     /translation="FTVIIAFVTVQMAWWIYFFSGYVGEVSHGTLDSLQREVEALNRM
                     LDAGDPGVAQLLNTRPHFRFDSSGRVVLDDATVTQFERDQNKVVRMLAFEGPFFVLVV
                     MTGLFIIARSLRVERELKQRQQNFLDAVGHEYKTPLGSLRLMVETLQLRQVTPEKLQE
                     YLSTMGSEIDRLEQTAQQVLATARLETDAPHLAMEPHELGALVTELVEGALPSLAGRG
                     GDVRLHKSEEPLFVTADRGLMAVLLENLLDNAVKYTPGPHKLVEVHVRRDGSAALVEV
                     DDRGVGIPEKERRLVFDRFYRVGNELTRTSPGVGLGLYLVKRAAESLGGTVDIEARPG
                     GGTRASVWLPLLVPVTEEAERPVTDA"
BASE COUNT          219 a          447 c          427 g          237 t
ORIGIN      
        1 gctccaacgg gctaccgcct ccgatgtcgg agtagttctt ctgcacggac ttcgtgcggc
       61 ggccagcgat gaagggcccg agagtggact gcgcgggaag ctggatgatc tcgcggtccg
      121 cgaagagctc catcaagaac ggcttcacct cgtccaggtt ccgtgggccg cccaagttga
      181 gcatgacgat gccggttggg ggacggttca tgccggccac cctaacttcg ctcggtcggc
      241 gcaagcgtca cgcatctgtc accggccgct cggcctcttc cgtgaccggt accagtaacg
      301 gcaaccatac gctcgcacgc gttccgcccc ctggccgggc ctcgatgtcg acggtgccgc
      361 ccagcgactc cgcggcgcgc ttcactaggt aaaggcctag gccgactccc ggggaagtgc
      421 gcgtcagctc gttgccgacg cggtagaacc ggtcgaagac caggcggcgc tccttctccg
      481 ggatgccgac gcccctgtcg tcgacctcga ccagagccgc gctgccgtcc cgccgcacat
      541 gcacctccac gagcttgtga ggccccggcg tgtatttcac ggcgttgtcc agcaggttct
      601 cgagcagcac ggccatcagg ccccggtcgg cggtcacgaa cagaggctct tccgacttgt
      661 gaagccgcac gtcgcccccg cggcctgcca ggctcggcag cgctccctcc accagttcgg
      721 tgaccagcgc gcccagttcg tgcggctcca tcgccaggtg cggcgcatcg gtctccaaac
      781 gggccgtggc gagcacctgc tgggccgtct gctcgagccg gtcgatctcg gagcccatgg
      841 tacttaggta ctcctgaagc ttctcgggcg tcacctggcg gagttgcagc gtttcgacca
      901 tgagcctcaa gctccccagc ggcgtcttgt actcgtgtcc gacggcgtcg aggaagttct
      961 gttgacgctg tttcagttca cgctcgacgc gcaggctgcg ggcgatgatg aatagacccg
     1021 tcatcaccac gagcacgaag aacggtccct cgaaggccaa catgcggact accttgttct
     1081 gatcgcgctc gaactgggtc acggtggcgt cgtcgagcac cacgcggccg gagctgtcga
     1141 aacgaaaatg cgggcgcgtg ttgagcagtt gcgcgactcc cgggtcgcca gcgtcgagca
     1201 tgcggttgag ggcctcgacc tcgcgttgga gcgagtctag ggtgccgtgg ctgacctcgc
     1261 cgacgtagcc gctgaagaag tagatccacc acgccatctg cacggtgacg aacgcgatga
     1321 tgacggtgaa
//
LOCUS       DAOQYE010000750         1327 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_130219_length_1327_cov_2.372642, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000750 DAOQYE010000000
VERSION     DAOQYE010000750.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1327
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130219_length_1327_cov_2.372642"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="PKN52_10780"
     CDS             <1..547
                     /locus_tag="PKN52_10780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00480.1"
                     /translation="TARKDAGSTTPGRPAQSAAAPARPTAKPTTLPPAHEGGLVSGQQ
                     SGRLVTSGPSGGQLVSGGTPSAGLGREGASAPLPESEPLQVTRRRVDPRRAVDSHEQV
                     VRPGSRTPDGRQGAKTGTATLGAPGVRREPGGRGSHGASHGPVFLGAGRDDLLRGMIW
                     HEVLSEPAAVKRLRRTRSRLR"
     gene            616..>1327
                     /locus_tag="PKN52_10785"
     CDS             616..>1327
                     /locus_tag="PKN52_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HNR00481.1"
                     /translation="MRTRLRAKVVARGDELRLSGEPAEVARAERAFRNLLETIRQGGE
                     VSLAEVAFGEFDGDAAGADAHAGGADLPGEGGGPARPGEHTDGLQHGADARLPGRTRP
                     KTAGQRRYVNAIARSPITFGLGPAGTGKTYLAVAMAVEALVANRVKRIILTRPAVEAG
                     ERLGFLPGDLQAKIDPYLRPLYDALHDMLPADKVERLLEQGTIEVAPLAFMRGRTLND
                     AFIILDEAQNTTPEQMKMF"
BASE COUNT          207 a          491 c          471 g          158 t
ORIGIN      
        1 gaccgcgcgg aaggacgccg ggtcgaccac gcccggtcga ccggcgcagt cggcggccgc
       61 gccggcccgc ccgaccgcga agcccaccac gcttccaccg gcacacgaag gtgggctggt
      121 cagcggccag cagagcggtc ggctcgtgac gagcggcccc tcgggcggcc aactcgtgtc
      181 cggcggcacc ccgtccgccg ggctcgggcg cgagggcgcc tcggctccgc tgccggagtc
      241 cgagccgctt caggtcacgc gcaggcgggt ggacccgcgg cgggccgtcg actcccacga
      301 gcaggtcgtg cggcccggct cgcgcacgcc tgatgggaga cagggggcca agacaggaac
      361 cgctacactg ggcgcgccag gcgtcaggcg ggaaccgggg ggtcggggct cgcatggcgc
      421 ctcgcacggc ccggtgttcc tcggcgccgg acgagacgac ctcctgcgcg gcatgatctg
      481 gcatgaggtg ctctccgaac cggcagccgt caagcgcctc aggaggacaa gatcgcgact
      541 tcgttaacgc tcaagctccg cagcccggcc gaagccctat cgctttacgg ccataacgac
      601 gagaacctca agctcatgcg cacgcgtcta cgcgccaagg tggtcgcgcg cggcgacgag
      661 ttgcgcctca gcggcgagcc agcggaggtc gctcgcgcgg agcgcgcgtt ccgcaacctg
      721 ttggagacca tccggcaggg cggcgaggtg agcctggccg aggtcgcgtt cggtgagttc
      781 gacggcgacg ccgccggcgc cgatgctcat gccggcgggg ccgacctgcc tggcgaaggc
      841 ggcgggccag cgcgcccggg cgagcataca gacggtctgc agcacggcgc cgatgcgcgc
      901 ctgccgggcc ggaccaggcc caagaccgcc gggcagcggc gctacgtcaa cgccatcgcc
      961 cgcagcccca tcaccttcgg gctcggcccc gccggcacgg gcaagaccta cctagcggtg
     1021 gccatggccg tcgaagcgct cgtcgccaac cgcgtgaagc ggatcatcct cacgcgcccc
     1081 gcggtcgagg cgggtgagcg cctcgggttc ctgccaggcg acctccaggc caagatcgac
     1141 ccgtacctca ggcccctcta cgatgctctg cacgacatgt tgccggccga caaggtggag
     1201 cgcctgctcg agcagggcac catcgaggtc gccccgctcg cgttcatgcg cggccgcacc
     1261 ctcaacgacg cgttcatcat cctcgacgag gcgcagaaca cgacgcccga gcagatgaag
     1321 atgttcc
//
LOCUS       DAOQYE010000751         1325 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_130490_length_1325_cov_3.279528, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000751 DAOQYE010000000
VERSION     DAOQYE010000751.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1325
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130490_length_1325_cov_3.279528"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="PKN52_10790"
     CDS             complement(<1..594)
                     /locus_tag="PKN52_10790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00482.1"
                     /translation="MSRRTSPLALLIAILALGAGVTHAQGFDENRYYQQCLRFEAGGD
                     LETARQACLNAIQIRPDFASAELALARIELGLGEVASAENHLRRVRNRIDTAEPIVLL
                     AEAALANHRPGDAEALLQEAKDRLATQVNVELQGRVAFLQGRIMQSQGRFAEALDAYA
                     DAILGNPLDVGYRLADARLRLRLGEPAAAEQQLQSYIN"
     gene            929..1309
                     /locus_tag="PKN52_10795"
     CDS             929..1309
                     /locus_tag="PKN52_10795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00483.1"
                     /translation="MSIAFFLMLGQAVFQLPAMPEGTEVRIVSVDLLTVYASAKVLDG
                     HLVFGEFPPPGTEVRVLIFPPDDDVRSKAAQLAGATAYKGTVTPGGADVQLAVEGRSR
                     PLSLREILLEEREIWLDLPIGRSL"
BASE COUNT          199 a          451 c          438 g          237 t
ORIGIN      
        1 gttgatgtag ctctgcaact gctgttcggc cgccgccggt tcgccaaggc gcagtctcaa
       61 cctggcatcc gccagccgat agccgacatc gagggggttg ccgaggatgg cgtcggcgta
      121 ggcgtcgagc gcctcggcga accgcccctg gctctgcatg atgcgcccct gcaggaacgc
      181 tacccgcccc tgcaactcga cgttcacctg agtcgcgagg cggtccttgg cctcttgcaa
      241 caaggcttcc gcgtcgcccg ggcggtggtt cgccagggcg gcctcggcca ggagcacgat
      301 cggctcggcg gtatcgatgc ggttacgcac gcggcgcaag tggttctcgg cgctcgccac
      361 ctctccgagg cccagctcta tgcgagcgag cgccagctcc gcggaggcga agtctggccg
      421 gatctggatg gcgttgaggc aggcctgacg ggccgtctct aggtcgccac ccgcctcgaa
      481 gcggaggcat tgctggtaat aacggttctc gtcgaaccct tgcgcatgcg taacgcccgc
      541 tccgagagcg aggatggcga taagcagcgc caaggggctc gtccgcctac tcaagagtgc
      601 ggctccccgg ttgcctgaca tgcgttccct tcccgctagt cgatgtctag cccggtctca
      661 tgatgcagcg gatcaccgcc gggaggaggt tacttcgttc actcgcgcct cgccaccacc
      721 atgggtggtc ggggtccggc gttcgcatgg tatgggcgct cggcccccgt cggaggggcg
      781 gtcgtgagcg ggcggtcgcc ggtacgcgcc gccgccgcgg gcggttaagg ttccctgaag
      841 ccgcttccct cgggccgttc acgcgccgct gatagcatct tctcatgacg tgcgctgagc
      901 atgggtgctg caacggcccg gtagcagaat gagcatcgcg ttcttcctga tgctcggcca
      961 ggcggtcttc cagctccctg ccatgcccga ggggactgag gtccgcatcg tctccgtcga
     1021 cctcctgacc gtgtacgctt ctgccaaggt cctggacggg cacctcgtgt tcggcgagtt
     1081 cccgccgcca ggcaccgaag tgcgagtgct catcttccca cccgacgacg acgtacgctc
     1141 caaggcggcg cagctcgcgg gcgcgaccgc ctacaaaggc acggtcacgc caggcggcgc
     1201 cgacgtgcaa ctggccgtcg aagggcggtc ccgacccttg tccttacgcg agatcctcct
     1261 ggaggagcgc gagatctggc tcgacctacc gataggaagg agcctctaag catgtctgcc
     1321 aaacg
//
LOCUS       DAOQYE010000752         1324 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_130713_length_1324_cov_2.412136, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000752 DAOQYE010000000
VERSION     DAOQYE010000752.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130713_length_1324_cov_2.412136"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="PKN52_10800"
     CDS             <1..187
                     /locus_tag="PKN52_10800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00484.1"
                     /translation="TGTALYPVVPDDEDKWSVSQLPGFEGNVCEVDTLIRLRVRVTDA
                     QGSVGEDYVVLRFTVIC"
     gene            complement(231..1262)
                     /locus_tag="PKN52_10805"
     CDS             complement(231..1262)
                     /locus_tag="PKN52_10805"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNR00485.1"
                     /translation="MDPRVNFITLAVADVNASRAFYVDGLGWQPIFDVPGEIVFIRLS
                     PTLVLSLWKADAFEEEVGPLNPRTGRSPFTLAYNVPAPELVDEALAVARAAGAVILKP
                     ATHRDWGGYSGYFTDPDGFAWEVAYNPGEIGVALMEAVAAGAEPTTVDVIRTRRSVPQ
                     GKLGGGPALDHREVVAAVESARWAPNHKRTEPWRFHLLDDDRIARLAELWGEQLERTG
                     SKPDRVEAKRRDWAEVPGALIVTCTSAADADDTLRLEDYAATACAVQNLCLHLWSKGI
                     ATKWSTAAVTDHEGFWPLLGLDGAPDGTRVVALLFYGLAAELPKAHRKLPVEHVLVDH
                     RHGARAEAR"
BASE COUNT          201 a          456 c          474 g          193 t
ORIGIN      
        1 caccggcact gccctctacc cggtcgtgcc ggacgacgag gacaagtgga gcgtctccca
       61 actccccggc ttcgagggca acgtctgcga ggtcgacacc ctcatccgtc tgcgagtgag
      121 ggtgaccgac gcgcagggaa gcgtgggcga ggattacgtg gtcctgcgct tcaccgtcat
      181 ctgctgaacc gagccgacca ctcccgtggc cgcgggccgg ggcgcgtttc tcagcgtgcc
      241 tcggcccgcg ccccgtggcg gtggtcgacg aggacgtgct cgacggggag cttgcggtgg
      301 gccttgggca actccgcggc gagcccgtag aagagcaggg cgacgacacg cgtgccgtcg
      361 ggcgcgccat ccaagcccag cagcggccag aagccctcgt gatccgtaac ggccgccgtg
      421 gaccacttgg tggcgatgcc ctttgaccat aggtgcaagc acaagttctg cacagcgcac
      481 gccgtggcgg cgtagtcctc cagcctcagg gtgtcgtcgg cgtccgccgc gctcgtgcaa
      541 gtgacgatga gcgcccccgg cacctcggcc caatcgcggc gcttggcctc cacgcggtcc
      601 ggcttcgaac cggtccgctc cagctgctcg ccccacagct cggcgagacg cgctatgcgg
      661 tcgtcgtcca ggagatggaa acgccacggc tccgtgcgct tgtggttcgg cgcccagcgg
      721 gcggactcga cggccgccac cacctcgcgg tggtcgaggg caggcccgcc gccgagcttg
      781 ccctgcggga cggagcggcg cgtacggatg acgtccacgg tcgtcggctc cgctccggcc
      841 gccaccgcct ccatgagggc gaccccaatc tcgccggggt tgtaggcgac ctcccacgcg
      901 aagccgtccg ggtccgtgaa gtagccggag tagccgcccc agtcgcggtg ggtggcgggt
      961 ttgaggatga cggcgccggc cgctcgcgcc accgccaggg cctcgtcgac cagttccggg
     1021 gcaggcacgt tgtaggccag cgtgaagggc gaccggccgg tgcgtgggtt gagcggaccg
     1081 acctcctcct cgaaggcgtc cgccttccag agggagagca ccagcgtcgg cgagagccgg
     1141 atgaacacga tctcgccggg gacgtcgaag atgggctgcc agccgagtcc gtcgacgtag
     1201 aacgcacgtg aggcgttcac gtcggcgacg gccaaagtta tgaagttgac tctagggtcc
     1261 acgccggatt gtatccgcgg gcagctgcgc cgacaggtac cgggtggcag agcgccgcgg
     1321 caag
//
LOCUS       DAOQYE010000753         1324 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_130759_length_1324_cov_2.058314, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000753 DAOQYE010000000
VERSION     DAOQYE010000753.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130759_length_1324_cov_2.058314"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /locus_tag="PKN52_10810"
     CDS             <1..540
                     /locus_tag="PKN52_10810"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HNR00486.1"
                     /translation="FLGKSFKNWRGMQASGGRRIKRSLDLDLSSVRFLTDDELEHLSR
                     FALLRPYLEEKRAEIAEWTARNPAAREDVLNSRRLTNAGTFRAYVTRYLRAHPQIAQD
                     MTFLVRQLQPGPTGLPLEVYVFVNDVRWVVYESVQSDIFEHLIAILPEFGLRLFQEPS
                     GSDMRALGEGAGGARDARA"
     gene            complement(595..>960)
                     /locus_tag="PKN52_10815"
     CDS             complement(595..>960)
                     /locus_tag="PKN52_10815"
                     /inference="COORDINATES: protein motif:HMM:NF008888.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HNR00487.1"
                     /translation="PKMQAALADGLDDGPEQTAARAEVRRLIEERIDLLPDAYRTVFM
                     LRAVEELSAADVAQALDIPEATVRTRFFRARRLLREGLTTDVAADLGEAFAFDGARCD
                     RIVENVLRRARLEGLSDAV"
     assembly_gap    961..970
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<971..1287)
                     /locus_tag="PKN52_10820"
     CDS             complement(<971..1287)
                     /locus_tag="PKN52_10820"
                     /inference="COORDINATES: protein motif:HMM:NF008888.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNR00488.1"
                     /translation="MAPRAPDQDCDTGLVARAAAGDEAAFEAIMRTYNQLLFRTARSI
                     LKSDAEAEDALQDAYLHAWRGLRSFRAESKLSTWLVRIVTNEALGRLRRAKVRTIPLE
                     DAMT"
BASE COUNT          181 a          455 c          475 g          203 t
ORIGIN      
        1 ttcctcggca agtcgttcaa gaactggcgc ggcatgcagg cgtccggcgg caggcgcatc
       61 aagcgcagcc tcgacctcga cctgtccagc gtccgcttcc tgacggacga cgagctggag
      121 cacctaagcc ggttcgcgct gctgcggccc tacctggagg agaagcgggc cgagatcgcg
      181 gagtggaccg cccgcaaccc ggcggcgcgc gaggacgtgc tcaactcgcg ccgcctgacg
      241 aacgctggca cgttccgggc gtacgtgacg cgctacctcc gcgcgcaccc gcagatagcc
      301 caggacatga cgttcctcgt gaggcagctg cagccgggac ccaccgggct ccccctggag
      361 gtctacgtgt tcgtcaacga cgtccgctgg gtcgtctacg agagcgtcca gtccgacatc
      421 ttcgagcacc tgatcgcgat cctgcccgag ttcgggctca ggctcttcca ggagcctagc
      481 gggagcgaca tgcgggcgtt gggcgagggc gctggtgggg cgagggacgc tcgggcctga
      541 cgcatcggcg cggcggtggg gcatcagggt cagcgtgttc cttccgtccc cgcctcagac
      601 cgcgtcgctc agcccttcca gtcgagcgcg gcgcagcacg ttctccacga tccggtcgca
      661 gcgcgcgccg tcgaaggcga acgcctcgcc gaggtcggcg gcgacgtcgg tcgtgaggcc
      721 ctcgcgcagg agccggcggg cgcggaagaa gcgcgtgcgg accgtcgcct ccgggatgtc
      781 gagggcctgg gccacgtccg cggcgctcag ctcctcgacg gcccgcagca tgaacaccgt
      841 gcggtaggcg tcggggagca ggtcgatgcg ctcctcgatg agacgccgca cttccgcgcg
      901 cgcggccgtc tgctccgggc cgtcgtcgag cccgtcggcg agcgccgcct gcatcttcgg
      961 nnnnnnnnnn gtcatggcgt cttccagtgg gatggtccgc accttagcgc ggcgcaggcg
     1021 gccgagcgct tcgttggtca ctatgcgcac gagccaggtg gagagcttgc tctcggccct
     1081 gaacgagcgc aggccgcgcc aggcgtgcag gtacgcgtct tgcagcgcgt cctccgcctc
     1141 ggcgtcgctc ttgaggatgc tgcgcgcggt gcggaagagg agctggttgt acgtccgcat
     1201 gatcgcctcg aacgcggcct cgtcgcccgc ggccgcacgg gcgacgaggc cggtatcgca
     1261 gtcctgatcg ggcgcccggg gcgccatagg aacgggttgg ctgatgatgg ctgactcccc
     1321 tgcc
//
LOCUS       DAOQYE010000754         1321 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_131222_length_1321_cov_2.778041, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000754 DAOQYE010000000
VERSION     DAOQYE010000754.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1321
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131222_length_1321_cov_2.778041"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            50..>985
                     /locus_tag="PKN52_10825"
     CDS             50..>985
                     /locus_tag="PKN52_10825"
                     /inference="COORDINATES: protein motif:HMM:NF021500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucodextranase DOMON-like domain-containing
                     protein"
                     /protein_id="HNR00489.1"
                     /translation="MILPTILALLALSVTDPSGDAFGDGTLSPPTAPIYADTAVFDIR
                     AVDLAAVDGGTRLSVSLGSLGLVGGDGVPDGGSAEPDGTEEQPVADPTDPAVADDRAE
                     LVGEVPLTGFLPTVIDVYLVGAGPGFSETLPGPDLDFPTGTAWQYAVRITSDGAQLVA
                     FPGAQAQPVEPRAEAASADAEAAASAGAEAGADVVDGAEPGEESGQNPEEAETVPAEL
                     GPADMPRLALSVSRQGRDLVVYLPMTLPDDTRVQAMSGVYDPFSPTGWRPLSPTPSPW
                     AFSGAEPQLSPVIDLLAADPGAQVAAITTGVLPRQS"
     assembly_gap    986..995
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1036..>1321
                     /locus_tag="PKN52_10830"
     CDS             1036..>1321
                     /locus_tag="PKN52_10830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00490.1"
                     /translation="MLPRQSEARFARFTPWPYVMVLGLLLALGGLVLRGRVKPSRVAT
                     GAAGVDGTGEATPSGGATGTAVAAVAAADPAAPAVGEADPTGQPEVEAGHD"
BASE COUNT          181 a          465 c          481 g          184 t
ORIGIN      
        1 acaggcatct caaggtgggt aagactgtac gggtgcccgg agtgagcctg tgatactgcc
       61 gacgatcctc gccctattgg cgctcagcgt caccgacccg agcggggacg cgttcggcga
      121 cgggaccctg tcgccaccta cggcgccgat ctacgctgac accgcggtct tcgacatccg
      181 ggcagtagac cttgcggccg tggacggcgg aacgcggctg agcgtctcgc tcggctcgct
      241 cggcctcgtg gggggtgacg gcgtccctga cggcggctct gccgagccgg acggcacgga
      301 ggagcagccg gtcgccgacc ccaccgatcc cgccgtcgcg gatgaccggg ctgagttggt
      361 cggcgaggtg ccgctcaccg gcttcctccc aaccgtcatc gacgtctatc tcgtcggagc
      421 cggccctggt ttctccgaga ccctgcccgg gccggacctg gacttcccca ccggcaccgc
      481 atggcagtac gcggtgcgga taaccagcga cggggctcaa ctggtcgcct tccccggcgc
      541 tcaggcccag ccggtcgagc caagagccga ggccgcgtcc gctgacgcag aagcggccgc
      601 atcggctggc gcagaagcgg gcgccgacgt ggtggacggc gcggagccgg gcgaggaaag
      661 cggccagaac cccgaggagg ccgagacggt gcctgccgag ctcggaccgg ccgacatgcc
      721 caggctcgcc ttgagcgtca gccgacaggg tcgagacctg gtcgtgtacc tgccgatgac
      781 actgcctgac gacacgcgcg tgcaggcgat gtcgggcgtg tacgatccgt tctccccgac
      841 gggatggcgc cccttgagcc ccacgccgtc gccgtgggcc ttctccggcg cggagccgca
      901 gctcagcccg gtcatcgatc tgctggccgc cgatccaggc gcccaggtcg ccgccatcac
      961 cacgggcgtg ctacccaggc agtcgnnnnn nnnnngcctg ggtagcacgc ccgtggtgat
     1021 ggcggcgacc tgggcgtgct acccaggcag tcggaagcgc gcttcgcccg gttcacgccg
     1081 tggccttacg tcatggtgct ggggctcctg ctcgcgctgg gcggcctcgt gctgcgcggc
     1141 agggtcaagc ctagccgcgt cgcgacgggc gcggccggcg tggacgggac cggagaggcc
     1201 actccgtctg gtggtgcaac cggtacggcg gtggccgccg tggcggcggc ggacccggcc
     1261 gccccggcgg tcggtgaggc cgacccgacc gggcagcccg aggtcgaagc aggtcatgac
     1321 g
//
LOCUS       DAOQYE010000755         1320 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_131398_length_1320_cov_2.871146, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000755 DAOQYE010000000
VERSION     DAOQYE010000755.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1320
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131398_length_1320_cov_2.871146"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1320)
                     /locus_tag="PKN52_10835"
     CDS             complement(<1..>1320)
                     /locus_tag="PKN52_10835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HNR00491.1"
                     /translation="WAFAAVGALESRERVQNDNPVNLSEQFLINKVKNDWARSDYTEG
                     YFATRALDVAAAVGQTIPPESLWTYNPAMKRASDDEGKAEDFAGTCDPYGTAGGGWCS
                     ETAHQSPVYCTTLIVFTFCGYQVMTSSSGVASSKTVQVWASGQTWNLNTYRNLLAQGH
                     VLLAGLKLYEGFWSPVGGVVSNYDKKYYDAKGDYVDGNYGGHDVQIVAFLSNADLSTP
                     SYTYDIGGGGYFVIKNSWGCGAGDGGYYYVPADYVESHFDDLYTLDFDSRRSAAWKKE
                     QATPGSMEAPAVTITDPRPTIDLRVGTDLTSFFGVTHSVAGSVSLYVRSDKDGVLFDG
                     AWNTSKYSFPSSLVRTFTSIGQRTISVRASFAGNVDEKSFVANVVNTAPTLGVDYSGT
                     AYISEFFPMAALVWDTNEAGIAGLCSRTTWSVSSPDTVSPASGCQVAV"
BASE COUNT          205 a          405 c          472 g          238 t
ORIGIN      
        1 cacagctacc tggcagccgg aggcgggcga gacggtgtcg ggggagctga cgctccacgt
       61 ggtgcggctg cagagccccg cgatgccggc ctcgttggtg tcccaaacga gcgcggccat
      121 ggggaagaac tcgctgatgt aggccgtgcc ggagtagtcg acgccaagcg tcggcgccgt
      181 gttcacgacg ttcgcgacga acgacttctc gtcgacgttg cccgcgaagc tggcgcggac
      241 cgagatggtg cgctggccga tcgaggtgaa ggtgcggacg agcgacgacg ggaagctgta
      301 cttgctcgtg ttccaggcgc cgtcgaagag gacgccgtct ttgtcggacc tcacgtacag
      361 gctgaccgaa ccggccaccg agtgggtcac gccgaagaag ctcgtgaggt cggtcccgac
      421 gcgcaggtcg atggtcggcc gcgggtcggt gatggtgacg gcgggagcct ccatgctgcc
      481 cggcgtggct tgctccttct tccaggcagc gctgcgcctg gagtcgaagt ccagcgtata
      541 gaggtcgtcg aagtgcgact ctacgtagtc ggccggcacg tagtagtagc cgccgtcgcc
      601 cgccccgcaa ccccaggagt tcttgatcac gaagtagcct ccgccaccga tgtcgtacgt
      661 gtaggacggc gtcgacaggt cggcgttgct gaggaaggcg acgatctgca cgtcgtggcc
      721 gccgtagttc ccgtccacgt agtcgccttt ggcgtcgtag tacttcttgt cgtagttgga
      781 caccacgccg ccgacgggcg accagaagcc ctcgtagagc ttgaggcccg cgaggagcac
      841 gtgtccttgc gcgaggaggt tgcggtaggt attgaggttc caggtctgac cggacgccca
      901 gacctggacg gtcttgctcg atgccacccc cgagctggag gtcatgacct ggtaaccgca
      961 gaacgtgaag acgatgaggg tggtgcagta gacggggctc tggtgggccg tctcggagca
     1021 ccacccgccg ccggcggtgc cgtacgggtc gcaggtgccg gcgaagtcct ccgccttgcc
     1081 ttcgtcgtcc gaggcgcgtt tcatcgcggg gttgtaagtc cagaggctct ccggggggat
     1141 cgtctggccg accgccgccg cgacgtccag cgcgcgcgtc gcgaagtagc cctcggtgta
     1201 gtcgctcctg gcccagtcgt tcttcacctt gttgatgagg aactgctcgg agaggttcac
     1261 ggggttgtcg ttctgcacgc gctcgcggct ctcgagtgcg ccgacggcgg cgaacgccca
//
LOCUS       DAOQYE010000756         1317 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_131968_length_1317_cov_2.183835, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000756 DAOQYE010000000
VERSION     DAOQYE010000756.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1317
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131968_length_1317_cov_2.183835"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            215..>1317
                     /locus_tag="PKN52_10840"
     CDS             215..>1317
                     /locus_tag="PKN52_10840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00492.1"
                     /translation="MVGSINWLRQAMEAHGANPNVVRNIIYRDKGRLPDKRALFLILD
                     ELWRAAGNAPLEMPELEAALSPTAGTEQEVLQLLGRDKRRAYRAMIGGIRTGELPKVL
                     VTGRPGSGKTLLTDYVRQGVEPLFDGGLDVIRLEFGGTDLATSLVRLATELGVPTDVM
                     ESRLVRIATASAFAVQADAQAELARVVLEAVRQRQAPILFLLHVSQSLASQDSLGLVP
                     LRLANPDVPRVSAVEWLWVTLFEPLARLPRVMLFVTITDLPARAMQRLGNFADPIRLT
                     PPTVTEARRFVKARVPDLSPGRQEEIVQRAGRSFEELRTLTLLAQARAPQEVGMQPDE
                     RYIEQLSGLVTAGDSDLRAFLAAIAVLSPPEHPA"
BASE COUNT          190 a          492 c          436 g          199 t
ORIGIN      
        1 aggctcatgg gcgagagtct aacatcggtc gcggaggact cccgtcccgg cggtgacgcg
       61 actcgcccac caggaactgc tgtgtactcg gacattggcg gcttggtaaa ctgaccgcat
      121 gcggagtcct tcgccctcag agccggtcag tccatggcgc cctctgttcg agagcgttcg
      181 ccaggccgtc ggccataagc gcgacgagca cggcgtggtc gggagcatca actggttgcg
      241 ccaggccatg gaggcgcacg gcgccaaccc caacgtcgtc aggaacatca tctaccgaga
      301 caagggcagg ctgcccgaca aacgcgcgct cttcctgatc ctcgacgagc tctggcgggc
      361 ggccggcaac gctccgctcg agatgccgga actcgaggcg gcgctctccc ccaccgccgg
      421 caccgagcag gaggttctcc agctcctcgg gcgcgacaag cgcagggcgt accgcgccat
      481 gatcggcggc atcaggaccg gcgagctgcc caaggtgctc gtcaccgggc ggccgggctc
      541 cggcaagacg ctcctgaccg actacgtccg ccagggcgtc gagccgttgt tcgacggggg
      601 cctcgacgtg atccgtctgg agttcggcgg caccgacctc gcgacctcgc tggttcgcct
      661 ggccacggag ctcggtgtgc cgaccgacgt gatggagtcg cggctcgtac gcatcgccac
      721 ggccagcgcg ttcgccgtcc aggccgacgc gcaggccgag ctggcgcgcg tcgtcctcga
      781 ggcggtgcgg cagcgccagg cccccatact gttcctgctc cacgtctcgc agtcgctcgc
      841 cagccaggac agcctcggtc tcgtgcccct ccgcctcgcc aaccctgacg tgccgcgcgt
      901 cagcgcggtc gagtggctat gggtcacgct cttcgagccg ctggcgcggc tgccccgcgt
      961 catgctgttc gtgaccatca cggacctacc tgcgcgcgcg atgcagcgcc tcggcaactt
     1021 cgccgacccc atccgcctga cccctcccac ggtcaccgag gcgcgccgtt tcgtgaaggc
     1081 gcgcgtcccc gacctcagtc cggggcgcca ggaggagatc gtgcagcgcg ccggccggtc
     1141 gttcgaggag ctgcggacgt tgacgctcct tgcgcaggcc cgcgccccgc aggaagtcgg
     1201 gatgcaaccg gacgagcgct acatcgagca gctctccggc ctcgtcacgg ccggcgactc
     1261 cgacctgcgc gccttcctgg cggccatcgc cgtgctctcg ccgcccgagc atccggc
//
LOCUS       DAOQYE010000757         1317 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_132011_length_1317_cov_1.760697, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000757 DAOQYE010000000
VERSION     DAOQYE010000757.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1317
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132011_length_1317_cov_1.760697"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="PKN52_10845"
     CDS             complement(<1..436)
                     /locus_tag="PKN52_10845"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00493.1"
                     /translation="MLFALRPQFKGYLYFGLATAFLSVYLASRSLAASALILNSELLV
                     RVEYVALFLMAPCFFAFIESLLYGRLSKPTPFIAAAFAVLALGSQVFRREPFLIIWYF
                     AALGSLVYYVGFVLGRSFVHELKARRATPKEERRGGLIFGSGS"
     gene            complement(322..>1317)
                     /locus_tag="PKN52_10850"
     CDS             complement(322..>1317)
                     /locus_tag="PKN52_10850"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNR00494.1"
                     /translation="GRARARGRTRGGVRGEGGRHAAVVPLAVAHGNARAMIGNALGVL
                     FDGVAYGSLLFVISIGLSVTMGLMNFVNLAHGAFAMLGGYACVMLMTKAGVPFLATLP
                     VAFAVAAVAGIVLERTLYVRLYKATPLDQVMFTIGLVFMAIAATTYFFGPSQQPVHLP
                     DFLRGQVQVAGIGLGAYRMFLIAVVAVITLVLHLLLVKTRFGAQVRASVDNAVASAGL
                     GINVNRVFGVTFALGSGLAGLGGALGICCVKRCNRWHPACKLPGFMAPWPSRPTRRRA
                     TSTSCSSASTLSSPSITSSSSRCGLSSRAIYTSAWLLPSSPCTWLLGASPPRRSS"
BASE COUNT          271 a          434 c          448 g          164 t
ORIGIN      
        1 ccgaacccga gccgaatatg agccctcctc ggcgttcttc cttcggcgtc gcccggcgcg
       61 ccttaagctc atgcacgaag gaccttccca ggacgaagcc gacgtagtag acgagcgagc
      121 ccagcgccgc gaagtaccag atgatcagga agggttcgcg cctgaaaact tggctcccga
      181 gcgccaagac ggcgaaggcg gcggcgataa agggcgtcgg cttactcaac cttccgtaga
      241 ggagcgactc gatgaaggcg aagaaacacg gcgccatcag gaagagcgcg acgtactcga
      301 cgcgcacgag gagttcggag ttcaagatga gcgccgaggc ggcgaggctc cgagaagcca
      361 agtacacgga gaggaaggca gtagccaggc cgaagtatag atagcccttg aactgaggcc
      421 gcagcgcgaa gaggatgagg tgatagaggg cgaagaaagc gtagatgccg atgagcatga
      481 ggtcgatgtc gctctgcgcc gtgtcggtct gcttggccac ggagccataa atccaggcag
      541 cttgcaggcg ggatgccagc ggttgcagcg cttcacgcag cagatgccca gcgcgccgcc
      601 gagacccgcg agacccgacc ccagcgcgaa cgtgacgccg aacacccggt tgacgttgat
      661 gccgaggccc gcggacgcga ccgcgttgtc gaccgacgcg cgcacctgcg ccccgaatcg
      721 cgtcttgacg agcagcaggt ggagcacgag cgtgatcacg gcgacgaccg cgatcaggaa
      781 catccggtag gcgcccagcc cgatgcccgc gacctggacc tgcccgcgca ggaagtcggg
      841 caggtgcacc ggctgctgcg acgggccgaa gaagtacgtc gtcgcggcga tcgccatgaa
      901 cacgaggccg atcgtgaaca tcacctggtc gagcggcgtc gccttgtaga gccgcacgta
      961 gagcgtgcgc tccagcacga tgcccgcgac ggccgccacg gcgaaggcga ccggcagcgt
     1021 cgcgaggaac ggcacgccgg ccttcgtcat cagcatcacg cacgcatagc cgcccagcat
     1081 ggcgaacgcg ccgtgcgcga ggttgacgaa gttcatcagg cccatcgtca ccgacaggcc
     1141 gatgctgatg acgaacagca ggctgccgta ggcgacgccg tcgaacagca cgccgagcgc
     1201 gttgccgatc atggcgcggg cgttcccgtg cgcgacggcg agcggaacga cggcggcgtg
     1261 cctgccgccc tccccccgca ccccgccccg tgtccggccc cgtgcacggg ctcgccc
//
LOCUS       DAOQYE010000758         1315 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_132235_length_1315_cov_3.113492, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000758 DAOQYE010000000
VERSION     DAOQYE010000758.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1315
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132235_length_1315_cov_3.113492"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..588)
                     /locus_tag="PKN52_10855"
     CDS             complement(<1..588)
                     /locus_tag="PKN52_10855"
                     /inference="COORDINATES: protein motif:HMM:NF013577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HNR00495.1"
                     /translation="MEPAGFQGSEALRLRVLQKYDGLSRAERRVADVALARGASLIDC
                     TIADLARESGVSQPTVVRFCNSIGFSGIKELKRAAVSVPAPAGSAGERVELDEVDSEE
                     KLISFVLDRMQDALRETYETFDRERLAAALDLLEDARFLRVVGTGGSAIAARHAQHYF
                     RRLGIPCSTFSVSEPEEVAMERYDHGDVVLAFSLSG"
     gene            complement(649..>1315)
                     /locus_tag="PKN52_10860"
     CDS             complement(649..>1315)
                     /locus_tag="PKN52_10860"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HNR00496.1"
                     /translation="KPFIDFLGITVPVALHVDHGSYDEALEAIGAGFKSVMFDGSALP
                     IEENLRRTAHLVDVCREAGVSLECEVGAIGGEEDGVAAMGECADPEECRLIAGLGMQM
                     LAAGIGNIHGKYPADWPGLRFDVLEEVGRVTGGIPLVLHGGSEIPDDMVRRAIGLGVC
                     KVNVNTECQLAFAAGVRRYIEEGRDLKGKGFATRALLADGLEASKRVAMNKMELFGSA
                     GKA"
BASE COUNT          211 a          476 c          425 g          203 t
ORIGIN      
        1 gccggacagg gagaaggcga ggacgacatc gccatggtcg tagcgttcca tggctacttc
       61 ctcgggctcg gagacggaga aggtggagca cgggatcccg agcctcctga agtagtgctg
      121 ggcatgccgc gcggcgatgg ccgagccccc ggttcccacc actcgcaaga accgagcgtc
      181 ctccaggagg tcaagggcgg cggccaaccg ctccctatcg aaggtctcgt acgtctcccg
      241 cagggcgtcc tgcatgcggt cgaggacgaa ggatatgagc ttctcctcgg agtcgacctc
      301 gtcgagctcg acgcgctcgc cggcggagcc ggccggggcc gggacgctga cggcggcgcg
      361 cttcagctcc ttgatgccgc tgaagccgat gctgttgcag aagcggacga ccgtcggctg
      421 gctcacgccg ctctccctcg ccaggtcggc gatggtgcag tcgatgaggc tcgctccacg
      481 cgccagcgcg acgtcggcga ccctgcgctc cgcacgactg aggccgtcgt acttctggag
      541 gacacgcagc cgcaacgcct cggagccttg gaagccagca ggttccacta ctctcctacc
      601 ctccgccgac cctgacgctc gggcgcggtc ggcgatccac gcccgatcct aagctttgcc
      661 ggcgctgccg aagagctcca tcttgttcat ggcgacccgc ttgctcgcct cgaggccgtc
      721 cgcgagcagc gccctggtgg cgaagccctt gcccttgagg tcacgaccct cctcgatgta
      781 gcgacggacg ccggccgcga aggcgagctg gcactcggtg ttcacgttga ccttgcagac
      841 cccgaggccg atcgcgcggc gaaccatatc gtccgggatc tccgagcccc cgtggagcac
      901 gagcgggatc ccgccggtga cccgacctac ctcctcgagc acgtcgaagc gcaaccccgg
      961 ccagtcggcg gggtacttgc cgtggatgtt cccgatgcca gccgccagca tctgcatgcc
     1021 gagcccggct atcaggcggc actcctccgg atcggcgcac tcgcccatcg cagccacgcc
     1081 gtcctcctcg ccgccgatgg cacccacctc gcactccagc gagacgcccg cctcacggca
     1141 gacgtccacg aggtgggccg tcctacgcag gttctcctcg atgggcagag ccgacccgtc
     1201 gaacatgacc gacttgaacc cggccccgat ggcctcgaga gcctcgtcgt agctgccgtg
     1261 gtcgacgtgc agggcgacgg gcacggtgat accgaggaag tcgatgaacg gcttc
//
LOCUS       DAOQYE010000759         1313 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_132558_length_1313_cov_3.550874, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000759 DAOQYE010000000
VERSION     DAOQYE010000759.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1313
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132558_length_1313_cov_3.550874"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(187..579)
                     /locus_tag="PKN52_10865"
     CDS             complement(187..579)
                     /locus_tag="PKN52_10865"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNR00497.1"
                     /translation="MILLDTHAWLWWLSDPGRLGRAAAERIELAKRDDRIVVSAISVW
                     EAALLVKKGRLELAIGMTALVRGCLALSFFRFEAIDPSIALGAVELSPLHPDPADRLI
                     VATALRLGVPLVTKDSRIQASGLVEAIW"
     gene            complement(576..857)
                     /locus_tag="PKN52_10870"
     CDS             complement(576..857)
                     /locus_tag="PKN52_10870"
                     /inference="COORDINATES: protein motif:HMM:TIGR01552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HNR00498.1"
                     /translation="MVTDYGHNVPGASARRMAMRTVSKSDLKPKVLEYLREVESTGEP
                     LFVTDRGRLVIRIEPYSDDDEALRLLQGVVLRYDDPTEPVAEDDWEALR"
     gene            999..>1313
                     /locus_tag="PKN52_10875"
     CDS             999..>1313
                     /locus_tag="PKN52_10875"
                     /inference="COORDINATES: protein motif:HMM:NF024744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HNR00499.1"
                     /translation="MTGTDVVPANPRFLPLTGTLNTRDLGGVAVPGHGTTRLGVFYRS
                     DVPMGLSVSDKGLPRELGLTTVIDLRQDHELERDANDLYGQVGSAYLRRAGMSERVLQ
                     RAR"
BASE COUNT          242 a          407 c          423 g          241 t
ORIGIN      
        1 gcggcaaagc cgcgcagctg ctcagccaag tgatccagca ggccggcgtg aggaggtagg
       61 ggtgggcggg atcgcggagg tcgtgcgggg ggtggtgggt gggaggggct gaaagtgacg
      121 cgaggggggg ctagcgtttg ttcttctatt ggtgaccacc gtggccaaac caccagagtc
      181 aaccgcctac cagatcgctt ctaccaaccc gctcgcctgg atgcgcgaat ccttggtgac
      241 gagcggcacg cctagtcgca gagccgtcgc gacgatcaac cggtccgcgg gatcgggatg
      301 caggggcgaa agctcgacgg cgcccaaggc gatggatgga tctatggcct cgaagcggaa
      361 gaaggaaagc gcaaggcaac ctcggacgag cgcggtcatg ccgatagcga gttccagccg
      421 gcccttcttg accagaagag ccgcttccca cacgctgatg gcggacacga cgatgcgatc
      481 atcgcgcttg gccagctcga tccgctctgc cgcggcccga ccgagccggc caggatcgct
      541 tagccaccag agccacgcat gcgtgtcgag caggatcacc gaagcgcctc ccagtcgtct
      601 tctgccaccg gctccgttgg gtcgtcgtat cggagtacga ctccctgcag cagtcggagc
      661 gcctcgtcgt catcgctata cggctcgata cggatcacca ggcggccccg gtccgttacg
      721 aacagtggct ctcctgtcga ctcgacttcg cgaaggtact cgagaacctt cggcttgaga
      781 tctgacttgg agactgttcg catcgccatc ctcctcgcgc tcgctccagg gacattatgc
      841 ccatagtctg tgaccatgga cataatacta ttatcaggat ggtttagcga tggtcgcggc
      901 ctccactggc acaccgttcg aatactcgcg tcgccgctgc gggatgcctc tcttggcgtt
      961 ggtcctgaag acctcgtgta gctctgtgta ctccgaccgt gacaggcact gacgtggtcc
     1021 cagccaaccc tcgcttcctt cccctgaccg gcacgctcaa cacgagggac ctgggagggg
     1081 tagcggtgcc tggccatggc actacgcgcc taggtgtctt ctaccgttcc gacgtcccga
     1141 tggggctgag cgtttccgac aagggtctgc ctcgcgagtt gggcctgacc accgtgatcg
     1201 atctccggca ggatcacgag ttggagcgcg acgccaacga cctatacggt caagtcggga
     1261 gcgcctacct aaggcgggca gggatgtccg agcgagtctt gcaacgggca agg
//
LOCUS       DAOQYE010000760         1312 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_132858_length_1312_cov_1.715990, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000760 DAOQYE010000000
VERSION     DAOQYE010000760.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1312
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132858_length_1312_cov_1.715990"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..521
                     /locus_tag="PKN52_10880"
     CDS             <1..521
                     /locus_tag="PKN52_10880"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNR00500.1"
                     /translation="YIPTQESCKTMSGVQVVYQPGQRYIGAQNQLLLPEGLTDYGALQ
                     AIDVATGKVAWRVDQKLPVVSPPLSTHGGLVFIGDAASREFRAFDAKTGKELWHFTTN
                     SSVVGVPTSFEVDGVQYVAVESSGGNTMTNVLAQTAKLANVPFSAPPQGGVIWVFALK
                     DKAAAAQAAAAQ"
     gene            656..>1312
                     /locus_tag="PKN52_10885"
     CDS             656..>1312
                     /locus_tag="PKN52_10885"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HNR00501.1"
                     /translation="MREPTTALRRLFALALVVAVSGAFAFAQDAQPTWELVVCAAKDD
                     LPYSNQEGQGFENKIMSVMAADLHADLSYIWLPGIQVPRENRRLLSEGKCDVLLDVGD
                     AADGFLTTLAYYQTTYYFVYRDDAPFEVASFDDDVLAGLRIGVVIASPPDEALGERGL
                     ATTAVHYSPTPVSPEAKMLDDVAAGALDLAIVYGPSAWTLAKSHPGLQLVPVAPEVDL
                     A"
BASE COUNT          211 a          460 c          438 g          203 t
ORIGIN      
        1 cctacatccc cacgcaggag tcgtgcaaga cgatgtcggg cgtgcaagtc gtctaccagc
       61 ccggccagcg ctacatcggc gcgcagaacc agctcctgct gccggaaggg ctgaccgact
      121 acggggccct gcaggctatc gacgtcgcaa cgggcaaggt ggcgtggcgc gtcgatcaga
      181 agctgcccgt cgtgtcgccg cccctctcca cgcacggtgg cctcgtgttc ataggtgacg
      241 cggccagccg tgagttccgc gccttcgacg ccaagacggg caaggagctg tggcacttca
      301 ccacgaactc gtcggtggtc ggcgtgccga ccagcttcga ggtcgacggc gtgcagtacg
      361 tggcggtcga gtcgagcggc ggcaacacca tgacgaacgt gctggcgcag acggcgaagc
      421 tcgccaacgt gccgttctcc gctcccccac aaggcggcgt gatctgggtg ttcgcgctga
      481 aggacaaggc tgccgcggcg caagcggcgg cggcacagtg aagagcacgg gcggccgcgc
      541 ggcgcgagcc ggctgacgtc cgagcaccgc actctcaggc gggacggccc attggccgtc
      601 ccgccggcta ccccgtcacc gccccggcgg tgcggccgaa ggttgcgtgc tgcagatgag
      661 agagccaact actgccctac ggcgcctgtt cgcgctcgcc ctggtcgtgg cggtctcagg
      721 cgccttcgcc ttcgcgcagg acgcccagcc gacgtgggag ttggtggtgt gcgccgccaa
      781 ggacgacctg ccgtactcca accaagaggg gcagggtttc gagaacaaga tcatgtcggt
      841 catggcggcg gacttacacg ccgacctcag ctatatctgg ctacccggga tccaggtacc
      901 ccgcgagaac cgcaggttgc tcagcgaggg caagtgcgac gtcctcctcg acgtgggaga
      961 cgccgccgac ggcttcctaa ccacgctcgc ctattaccag acgacctact atttcgttta
     1021 ccgcgacgac gctccgttcg aggtcgcgtc cttcgacgac gatgtgctcg ctggcctccg
     1081 catcggcgtg gtgatcgcca gcccgccaga cgaggcgttg ggtgagcgcg gactggcgac
     1141 caccgccgtg cactactcgc cgacgccggt gagccccgag gcgaagatgc tcgacgacgt
     1201 ggccgccggc gcgctcgacc tggccatcgt ctacggtcct tccgcctgga cgctcgccaa
     1261 gtcgcacccc ggcctgcagc tcgtgcccgt ggcacccgag gtcgacctcg cc
//
LOCUS       DAOQYE010000761         1311 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_132996_length_1311_cov_2.296975, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000761 DAOQYE010000000
VERSION     DAOQYE010000761.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132996_length_1311_cov_2.296975"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(60..518)
                     /gene="argR"
                     /locus_tag="PKN52_10890"
     CDS             complement(60..518)
                     /gene="argR"
                     /locus_tag="PKN52_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HNR00502.1"
                     /translation="MPSKEQRQRLIAELVQEEFISTQAEIAERLAQSGIKVTQATISR
                     DVTELRLVRVPSGRGRHRYSATPLVMQDDVERELRDCFKRFVRNLDRGENVLVVSTDE
                     GHAAGVAFVIDKLEREEIVGTLAGQNAILVVARTVAVATELLAEFEGLLD"
     gene            complement(722..1027)
                     /locus_tag="PKN52_10895"
     CDS             complement(722..1027)
                     /locus_tag="PKN52_10895"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF013365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HNR00503.1"
                     /translation="MAQEGYDKLISLTDSRYRLSMIVARRAAQLKMGLPTMLAPGTLD
                     SLENSVTVAMKEVETGAGIVWGDDLPVQDELKRLVEVPKREPSNAYVGTPFDDEDDD"
BASE COUNT          197 a          469 c          406 g          239 t
ORIGIN      
        1 ctgacgctgg ccggcgtgct cgacgcgatc gactcgacgc tggaagacgc gagcgccctt
       61 cagtcgagca gtccctcgaa ctccgcgagt agctccgtcg ctacggcgac cgtgcgagcg
      121 acgacgagga tggcgttctg gccggccagc gtcccgacga tctcctcgcg ctcgagcttg
      181 tcgatgacga acgcgacgcc ggcggcatgt ccctcgtccg tgctcacgac gaggacgttc
      241 tctcccctat ccaggttgcg cacgaagcgc ttgaagcagt cgcgcagttc gcgctcgacg
      301 tcgtcttgca tgacgagcgg cgtggcgctg tagcggtgcc tgcctcgccc cgacggcacg
      361 cgcacgaggc gcaactcggt cacgtcgcgg ctgatagtcg cctgcgtcac cttgatcccg
      421 ctctgggcaa ggcgctccgc gatctccgcc tgggtcgaga tgaactcctc ctgcacgagc
      481 tcggctatca gtctctggcg ctgctccttg ctgggcatga atattcattc tagcatccgg
      541 aaccttacgc acgcggagcg tcgcgacgga cgcctccgga ccgtgcccct gccttcacca
      601 cggtgcccca ggcaccgccc ggacacctcg tcacacctac ggcgccggca aacaacaccg
      661 ccgccggttc ctaaccggcg gcggcatctg ggcgggagcg acgttcgctc ggctcgctcg
      721 ttcagtcgtc gtcctcgtcg tcgaacggcg taccgacgta ggcgttcgac ggctcgcgct
      781 tgggcacctc tacgaggcgc ttcagctcgt cttgaacggg taggtcgtcg ccccagacga
      841 tgccggcccc ggtttcgacc tctttcatgg caacggtgac gctgttctca aggctgtcga
      901 gcgttcccgg cgcgagcatc gtcggcaggc ccatcttcaa ctgcgcggcc ctgcgcgcga
      961 cgatcatgga aagcctgtaa cgcgaatccg tcagggagat gagcttgtcg taaccttctt
     1021 gagccatctg gtgcctccgt ggtgcaacgc tcggtcgctg cccaggggct gggttccgag
     1081 cccgccaccg cgacgacgcg agtctgttcg ctactcccga gcgcccaagg cgctcgctag
     1141 acccgccagc ataacccaag cgccgctctt tcaccacggt atcgggcgtt cgcggccggc
     1201 gggcgttcgg cagccatgac ctcaagagcg caggtactgc tccagttcgg cgtccgtgac
     1261 gagccgcgcc cgcgcccgct ctgcgcggat gatcgattcg aactcctgga c
//
LOCUS       DAOQYE010000762         1310 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_133217_length_1310_cov_1.953785, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000762 DAOQYE010000000
VERSION     DAOQYE010000762.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1310
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133217_length_1310_cov_1.953785"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1005)
                     /locus_tag="PKN52_10900"
     CDS             complement(<1..1005)
                     /locus_tag="PKN52_10900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00504.1"
                     /translation="MARRPTAQRHMTVALLALTVTALAPLARAQATATVRVGAGKGFA
                     LPEHALSGFNYGNSMRVVGWDDLFAAIGVTSLRFPPGNIADEQPLNEAAVAALKVNWE
                     LLGEPEVTFVANAFTGSPEETVAAARLLQGAGIGVSLWEIGNEPDLYATNRLDPSWTP
                     ERYCAVFREHGAALRALVPGARLAGPGVSGARPGGLDYLREVLRLCGDVIDVLTFHVY
                     PTDGTWSDEAALATADLVSDELARIGSWLADPDANPLGHARRIDVGVTEFGLSWRTNN
                     FHHLEDMTAAIWLADTLGRLADGGVAISHYFALQAMGGHGLIDSGGWVRPTYHVYALL
                     A"
     gene            complement(1005..>1310)
                     /locus_tag="PKN52_10905"
     CDS             complement(1005..>1310)
                     /locus_tag="PKN52_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNR00505.1"
                     /translation="GATEFRIYWSVALPLIRPTLATLALLTFIGQWNNFLGPLVILRS
                     RTTFTLPLALRSLQGLINTDWGVVILGTALAVVPLLVLFFFASRQVIEGLTAGAVKG"
BASE COUNT          202 a          422 c          506 g          180 t
ORIGIN      
        1 cgccaggagc gcgtagacgt gataggtggg ccgtacccag ccgcccgagt cgatgaggcc
       61 gtggccgccc atcgcctgca gggcgaagta gtggctgatg gccacgcccc cgtcggcgag
      121 gcggcccagg gtgtcggcga gccagatggc ggcggtcatg tcctcgaggt ggtggaagtt
      181 gttcgtgcgc cacgacagcc cgaactccgt cacccctacg tcgatgcggc gcgcatggcc
      241 aagcgggttg gcgtcggggt cggcgagcca actcccgatg cgcgctagct cgtcgctgac
      301 gaggtcggcc gtggcaaggg cggcctcgtc gctccacgtg ccgtccgtcg ggtagacgtg
      361 gaaggtgagc acgtcgatga cgtcgccgca gaggcgcagg acctcgcgca ggtagtcgag
      421 gccgcccggc cgcgcgcccg atacgccagg tcctgcgagc cgcgctcccg ggacgagtgc
      481 gcgtaaggcg gcgccgtgct cgcggaagac ggcgcagtag cgctctggcg tccacgatgg
      541 gtcgagccgg ttggtggcgt agaggtcggg ctcgttgccg atctcccaga gggagacgcc
      601 tatgccggcg ccctgcagca gccgcgccgc cgcgaccgtc tcttcggggc tgccggtgaa
      661 cgcgttggcg acgaaggtca cctcgggctc gccgagcagc tcccagttca ccttgagcgc
      721 cgcgacggcg gcctcgttga gcggctgctc gtcggctatg ttgcccggcg ggaagcgcag
      781 cgacgtgacg ccgatggccg cgaagaggtc gtcccacccg acgacgcgca tggagttgcc
      841 gtagttgaag ccgctcaggg cgtgctcggg gagcgcgaag cccttgccgg cgccgacgcg
      901 cacggtcgcg gtcgcttgcg cgcgagcgag gggcgcgagg gcggtcacgg tcagcgccag
      961 cagcgccacc gtcatgtgcc gttgagccgt ggggcgacgt gccatcagcc cttgaccgcc
     1021 ccggccgtca agccctcgat gacctggcgc gaggcgaaga agaagagcac caacagcggc
     1081 acgacggcga gggccgttcc gaggatgacg acgccccagt ccgtgttgat caagccctgc
     1141 agggagcgta gggcgagggg gagcgtgaac gtcgtgcggc tgcgcaggat cacgagcggc
     1201 ccaaggaagt tgttccactg gccgatgaac gtgaggagcg cgagcgtggc gagggtcggc
     1261 cggatgagag gcaacgcgac gctccagtag atgcggaact cggtggcgcc
//
LOCUS       DAOQYE010000763         1309 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_133275_length_1309_cov_3.784689, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000763 DAOQYE010000000
VERSION     DAOQYE010000763.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1309
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133275_length_1309_cov_3.784689"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..763
                     /locus_tag="PKN52_10910"
     CDS             <1..763
                     /locus_tag="PKN52_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNR00506.1"
                     /translation="EREMRKIRGNDISMIFQEPMTSLNPVYTVGDQIAEAIVLHQGKS
                     HREAMKMAAEMLDLVGIPEPGKRVKNFPHQMSGGMRQRVMIAMALSCGPKLLIADEPT
                     TALDVTIQAQILDLMRKLQNEIGMSILFITHDLGVVAEMADRAVVMYAGRAVEEAHVN
                     DIFANPQMPYTLGLLNSIPRVDRAAEHQDRLMAIPGNVPNPLNLPQGCAFHPRCRFVQ
                     DKCKVAIPNLEDTGNGHMVRCVRWQELDLKAEIPA"
     gene            760..>1309
                     /locus_tag="PKN52_10915"
     CDS             760..>1309
                     /locus_tag="PKN52_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNR00507.1"
                     /translation="MSNQRSKDAKGAATESGPLLEVKNLRKYFPIRGGIFSRVVANVK
                     AVEDVSFNVERGEVVGLVGESGSGKTTVGRSILRLIEPSAGEVLFDGVDVTKLSKAHM
                     REYRKRMQIIFQDPFASLNPRMSVGDIIGEAMTIHNLARGKEREQRVAQLLERVGLSP
                     SHMRRYPHEFSGGQRQRIGIARA"
BASE COUNT          246 a          469 c          405 g          189 t
ORIGIN      
        1 cgagcgcgag atgcgcaaga tccgcggcaa cgacatctcg atgatcttcc aggagccgat
       61 gacgagcctc aaccccgtct acacggtcgg cgaccagatc gccgaggcca tcgtcctcca
      121 ccagggcaag agtcaccgcg aggccatgaa gatggcggcc gagatgctcg acctcgtcgg
      181 catcccggag cccgggaagc gcgtcaagaa cttcccgcac cagatgtcgg gcggcatgcg
      241 ccagcgcgtg atgatcgcca tggcgctgtc gtgcggtccg aagctcctga tcgccgacga
      301 gcccactacg gccctcgacg tgacgatcca ggcccagatc ctcgacctga tgcgcaagct
      361 ccagaacgag atcggcatgt ccatcctctt catcacgcac gacctcgggg tcgtggccga
      421 gatggccgac cgcgcggtgg tcatgtacgc cgggcgcgcc gtcgaggaag cgcacgtcaa
      481 cgacatcttc gccaacccgc agatgcctta caccctcggc ctgctcaact cgatcccccg
      541 tgtcgaccgg gcggccgagc accaggacag gctcatggcc atccccggta acgtgcccaa
      601 ccccctcaac ctgccccagg gctgcgcgtt ccacccgcgc tgccggttcg tacaggacaa
      661 gtgcaaggtg gccatcccca acctcgagga caccggtaac ggccacatgg tccggtgcgt
      721 caggtggcaa gagctcgacc tcaaagcgga gatcccggca tgagcaacca gcgcagcaag
      781 gacgccaaag gtgcggctac cgagtccggc ccgctcctcg aggtcaagaa ccttcgcaag
      841 tacttcccca tccgcggggg catcttctcg cgcgtggtgg ccaacgtcaa ggccgtcgag
      901 gacgtcagct tcaacgtcga gcgcggggag gtcgtcggcc tggtaggcga gtccggctcc
      961 ggcaagacga cggtcggccg cagcatcctg cgcctcatcg agccgtccgc cggtgaggtg
     1021 ctcttcgacg gcgtcgacgt caccaagctg agcaaggccc acatgcgcga gtaccgcaag
     1081 cgcatgcaga tcatcttcca ggaccccttc gcgagcctca acccgcgtat gtccgtcggt
     1141 gacatcatcg gcgaggccat gaccatccac aacctggcgc gcggcaagga gcgcgagcag
     1201 cgtgtggcac agctgctcga gcgcgtcggc ctctcgccgt cgcacatgcg ccggtacccg
     1261 cacgagttct cgggcgggca gcgccagcgc atcggcatcg cgcgcgcgc
//
LOCUS       DAOQYE010000764         1309 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_133369_length_1309_cov_2.178628, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000764 DAOQYE010000000
VERSION     DAOQYE010000764.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1309
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133369_length_1309_cov_2.178628"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="PKN52_10920"
     CDS             <1..208
                     /locus_tag="PKN52_10920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00508.1"
                     /translation="YNNFNSIWLMTEGGPLRATETLTILTYKLAFRNFDMGSATAAGV
                     LTFFVLLVFVVVFGRQYVKSQMEF"
     gene            255..1055
                     /locus_tag="PKN52_10925"
     CDS             255..1055
                     /locus_tag="PKN52_10925"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNR00509.1"
                     /translation="MLVAFFVCFVFAIPFLWMILTSLKLPSELFTSPVQVFPRSGVYW
                     EAYTRIWTAGQFGRYFWNSTLVATIATGASVALSVFAAIGFARYKLKGGNYLLLGVLL
                     SQLFPLVLLVPPFYTVLRELRILDTHLALVIAYTSFSLPFSIWMLTGYFRSIPFELEE
                     SAMIDGATRLGAQLRVVLPLAGPGIAATVIYSFILAWNEFLFATTFISSPDLRTLPIG
                     LQAFIGQYSTDWNLLMAGAVVTTVPVIVMFVFLQRYLVAGLTAGAVKG"
     gene            1052..>1309
                     /locus_tag="PKN52_10930"
     CDS             1052..>1309
                     /locus_tag="PKN52_10930"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNR00510.1"
                     /translation="MITKLSPPDWRAALAVTGKVAGTSQSEHAHNTLFQAIVQGRLGP
                     GCHLAEQPIADTIGVSRISVRDAIRQLESAGLVSVYPHRGAF"
BASE COUNT          182 a          474 c          391 g          262 t
ORIGIN      
        1 ctacaacaac ttcaactcca tctggctcat gacggagggc ggtccgctgc gcgccaccga
       61 gacactgacc atcctcacct acaagctagc cttccggaac ttcgacatgg gtagcgccac
      121 cgcggccggc gtcctgacct tcttcgtgct cctcgtcttc gtggtcgtct tcgggcgcca
      181 gtacgtgaag tcgcagatgg agttctgacg tggcatccgc caggctgcgt cgccgcacga
      241 ggcagctacc ggccatgctg gtcgccttct tcgtctgctt cgtcttcgcc atcccgttcc
      301 tgtggatgat cctgacgtcg ttgaaactgc catcggagct cttcacgagc ccggtgcagg
      361 tcttcccgcg ctcgggcgtc tactgggagg cctacacgcg catctggacg gccggccagt
      421 tcggccggta cttctggaac agtacgctcg tggcgaccat cgccaccggc gcgtctgtcg
      481 ccctgtccgt cttcgcggcc atcgggttcg cgcgctacaa gctcaagggc ggcaactacc
      541 tgctccttgg cgtactcctc agccagctct tccccctggt gctcctcgtg ccgccgttct
      601 acacggtgct gcgcgagctg cgcatcctcg acactcacct ggcgctcgtc atcgcgtaca
      661 cgagcttctc gctgccgttc agcatctgga tgctgacggg ctacttccgc tccatcccct
      721 tcgagctcga ggagtccgcc atgatcgacg gcgcgacccg cctcggtgct cagctcaggg
      781 tggtcctccc gctggccggc cccggcatcg ccgccaccgt catctacagc ttcatcctcg
      841 cctggaacga gttcctcttc gccacgacgt tcataagctc gccggacctg cggacgttgc
      901 cgatcggctt gcaggcgttc atcgggcagt acagcaccga ctggaacctg ctcatggccg
      961 gggccgtcgt cactacggtg cccgtgatcg tcatgttcgt gttcctgcag cgctacctgg
     1021 tggccgggct cacggccggg gcggtgaagg ggtgatcacg aagctctcgc cgcccgactg
     1081 gcgcgccgcc ctcgccgtta cggggaaggt ggcgggcacc tcccagagcg agcatgccca
     1141 caacacgctc ttccaggcca tcgtccaggg gcgacttggc cctggctgcc atctggcgga
     1201 gcagccgatc gccgacacca tcggcgtgag ccgcatctcg gtgcgcgacg ccatccgcca
     1261 gttggagtcg gcgggcctgg tgagcgtcta cccgcaccgg ggcgcgttc
//
LOCUS       DAOQYE010000765         1307 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_133741_length_1307_cov_2.032748, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000765 DAOQYE010000000
VERSION     DAOQYE010000765.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1307
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133741_length_1307_cov_2.032748"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="PKN52_10935"
     CDS             complement(<1..427)
                     /locus_tag="PKN52_10935"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNR00511.1"
                     /translation="MSRHVVVIGAGVIGLMSAYHLRRRGFDVTVLDREGSERSTASYG
                     NAGMIVPSHFVPLAAPGVVVQGLRWMANPKSPFYVKPRLSADLLKWGWRFWRSGTAAH
                     VAASAPLLLRLNLASRDEYAKLDDELGGGIGLERLGLTML"
     gene            complement(424..>1307)
                     /locus_tag="PKN52_10940"
     CDS             complement(424..>1307)
                     /locus_tag="PKN52_10940"
                     /inference="COORDINATES: protein motif:HMM:NF017365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proline racemase family protein"
                     /protein_id="HNR00512.1"
                     /translation="DLGTGPLAERLRRFRAEHDAFRSAIVNEPRGSDVLVGALLCEPD
                     DPAAAAGVIFFNNVGYLGMCGHGTIGLVATLAYQGRLGPGRHLIETPVGNVTAELHED
                     GRVSVYNVRAYRRLAGVRVNVEWRGERVDLTGDVAWGGNWFFLVSDPVERFGVSVSTS
                     DLEALTELAWRIKQALAATGITGDGGAEVDHVELFVPGEDGADSRSFVLCPGKAYDRS
                     PCGTGTSAKLACLAADGLLAPGETWVQESVIGSRFEGRYAAAETGVLPVITGRAHVTA
                     EGTLIIDPADEFAWGIA"
BASE COUNT          201 a          473 c          456 g          177 t
ORIGIN      
        1 agagcatggt gagtccgagc cgctccaggc cgatgccgcc gcccagctcg tcgtcgagct
       61 tcgcgtactc gtcgcgactg gcgaggttga gccgcaacag gagcggcgcg ctcgccgcga
      121 catgcgctgc cgtgccggag cgccagaagc gccaacccca cttgagcagg tcggctgaca
      181 gtcggggctt cacgtagaaa gggctcttcg ggttggccat ccagcgcagc ccctgcacca
      241 ctacgcccgg cgccgccagt ggcacgaagt gactcggcac gatcatgccg gcgttgccgt
      301 aggacgcggt ggagcgctcc gaaccctcgc ggtcgaggac ggtgacgtcg aagccgcgcc
      361 tgcgcaggtg gtaggcgctc atgaggccga tgacgccggc gccgatgacg acgacgtgcc
      421 tactcacgcg atcccccagg cgaactcgtc cgccgggtcg atgatcagcg tcccctcggc
      481 cgtgacgtgc gcgcggccgg tgatcaccgg gaggacgcct gtctccgccg ccgcgtaccg
      541 cccctcgaac cggctcccga tcacgctctc ctgcacccag gtctcccccg gcgccaacag
      601 gccgtcggcg gcgaggcacg ctagcttcgc gctcgtgccc gtgccgcacg gcgagcggtc
      661 gtaggccttg ccggggcaga gcacgaacga gcgcgagtcg gcgccgtcct ctcccggcac
      721 gaagagttcg acgtgatcga cctcagcgcc gccgtcgccc gttatgccgg ttgcagccaa
      781 agcctgcttg atgcgccagg cgagctcggt cagggcctcg aggtcggagg tcgacacgga
      841 cacgccgaac cgctcaacgg ggtcggacac gaggaagaac cagttgccgc cccaggccac
      901 gtcgcccgtc aggtcaacgc gctcgccgcg ccactctacg ttcacccgca cgccggccag
      961 gcggcggtag gcgcgcacgt tgtacacgct cacgcggccg tcctcgtgca gctcggcggt
     1021 cacgttgcca acgggcgtct cgatgaggtg cctgccgggc cccagtcggc cctggtacgc
     1081 gagcgtggcc acgagcccga tggtgccgtg cccgcacatg ccgaggtagc caacgttgtt
     1141 gaagaagatg acgccggccg cggcggcggg gtcgtccggc tcgcacagga gggcgccgac
     1201 gagcacatcc gagccgcgcg gctcgttgac gatggccgag cggaaagcgt cgtgctcggc
     1261 gcggaaacgc cgtaggcgct cggccaacgg ccccgtgccg aggtcgg
//
LOCUS       DAOQYE010000766         1306 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_133914_length_1306_cov_1.909672, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000766 DAOQYE010000000
VERSION     DAOQYE010000766.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1306
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133914_length_1306_cov_1.909672"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            159..533
                     /locus_tag="PKN52_10945"
     CDS             159..533
                     /locus_tag="PKN52_10945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00513.1"
                     /translation="MAGIVFLKTAQLERVRAFYTEQVGMTVWLEQPDIVILRHGNLLV
                     GFHRQPAADLDALITFVYETREEVDRMHARLRDVATSEPKENPKYRIYHFFGRDPEGR
                     RIEFQQFLHPVLPVTDTRDQSA"
     gene            936..>1306
                     /locus_tag="PKN52_10950"
     CDS             936..>1306
                     /locus_tag="PKN52_10950"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNR00514.1"
                     /translation="MTTISILYDNRAPSELRPGWGFSAWINVGDESVLFDTGADKLVF
                     EHNARALGCNVSTLGALVLSHDHCDHIGAVSAVTRKGLRLYVHAGSARRFAGHRRGGV
                     QVIGVRGPTQICPGVKSTGRFG"
BASE COUNT          207 a          440 c          420 g          239 t
ORIGIN      
        1 ccggtgacca acaccgggcc tggtctgtct gtcatgcgtg ctcctccacg cggagtcgat
       61 cgcgccctcc gccggccatg ggtcgatggc gccacgatac ggctccaccg tctcgcgacc
      121 gtctgtgtct aggcctccac tcaggctaca cttccctcat ggccgggatc gtattcttga
      181 agaccgcgca actcgagcga gtacgagcgt tctacacgga gcaggtgggc atgaccgtct
      241 ggctcgaaca gcccgacatc gtcatcctcc ggcacggcaa cctcctcgtc ggtttccatc
      301 gccagcccgc cgccgatctc gacgcgttga tcacattcgt ctacgaaacg cgcgaggagg
      361 tcgatcggat gcacgcccgc ctccgcgacg tcgcaacgag cgagcccaag gagaacccga
      421 agtataggat ctaccacttc ttcggcagag atccggaagg ccgccggatc gagttccagc
      481 agttcctgca ccccgtccta cccgtaaccg acacgaggga ccagtccgcc tgacggagat
      541 cgctccgtgc gcagttcgtc ttcccgtagg tgtccggcga gaagacgtca gccggtgtgc
      601 ttgccgttgt ctctagccgt tccccggcgc ggcccgcggt atagcaggct ctatgccgta
      661 ccgggatacc gcgagaccga cgtgccgatg acaaggaccg tggtcgccta gatgggtgcg
      721 cgtagcgcgc ctgagtagtg ggtaggcatc aaccctcagc caggcgggag gctgccctac
      781 acctcgcgag tcggctgtag gctgagttca tccgcgcgat ggcaggcgac gaagtgctcg
      841 tggccgaggt cgcgatgcac cggcacctcg cctgagaacg cctgggttgc gtccgggcgg
      901 cgctcccact aggcttctcc cgtgggaggg gtcccgtgac aacgatctcg attctctacg
      961 ataaccgcgc tccgtcggag ctgcgtcctg gctggggctt ctcggcgtgg atcaacgttg
     1021 gcgacgagtc ggtgctgttc gataccggcg ccgacaagct cgtgttcgag cacaacgcgc
     1081 gcgccctcgg ctgcaacgtc agcaccctgg gcgcgctcgt gctgtctcac gatcactgcg
     1141 atcacatcgg cgccgtatcg gcggtcacgc gcaaggggtt gcgcctgtac gttcacgcgg
     1201 gctccgctcg gcggtttgcc gggcatcgac ggggcggggt tcaggtgatc ggcgtccgtg
     1261 gccctacgca gatctgcccg ggcgtgaagt cgacgggccg cttcgg
//
LOCUS       DAOQYE010000767         1303 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_134381_length_1303_cov_2.925481, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000767 DAOQYE010000000
VERSION     DAOQYE010000767.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1303
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134381_length_1303_cov_2.925481"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="PKN52_10955"
     CDS             <1..370
                     /locus_tag="PKN52_10955"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177614.1"
                     /note="an AccC homodimer forms the biotin carboxylase
                     subunit of the acetyl CoA carboxylase, an enzyme that
                     catalyzes the formation of malonyl-CoA, which in turn
                     controls the rate of fatty acid metabolism; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HNR00515.1"
                     /translation="SDLVVRGHAIELRINAEDPDHDFRPSAGLITDVHWPGGPGIRVD
                     SHVYSGYRIPPNYDSLIAKIIAWAPSREEAIARMERALKETVVEGVKTTIPFHLKVLG
                     NAFFRRGDVYTNFIARRMGS"
     gene            367..840
                     /locus_tag="PKN52_10960"
     CDS             367..840
                     /locus_tag="PKN52_10960"
                     /inference="COORDINATES: protein motif:HMM:NF015721.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HNR00516.1"
                     /translation="MTEGPDLVIAGDVLLNIAQLALDRVEGVRPTQPPSSVSDLLGGR
                     RARGIAIERDGEDVWVDLTLAVECGVEIPKVAKAAQRAVREGMTSMTGLHVRSVNVVI
                     ESVEPSQASVGAGAATDAEAATSAESATGAAGQAAQDRDPWERETAREGGPATSE"
     gene            833..>1303
                     /gene="nusB"
                     /locus_tag="PKN52_10965"
     CDS             833..>1303
                     /gene="nusB"
                     /locus_tag="PKN52_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HNR00517.1"
                     /translation="MSRQRARELAFRTLFQSERGAEPLLEVWRNVRVELAEEAAESPE
                     DAVYGGALGLEDLAFAEGLLQAFDEHREELDARLATAITGWSFGQMAQTDLNVLRIAS
                     AEMRYGSEAPEVAIEMAVRLAKKFGGDESGRFVNGVLAKLIRDGEGRIPAAVSAE"
BASE COUNT          205 a          421 c          490 g          187 t
ORIGIN      
        1 gtccgacctg gtcgtgcgtg gccacgccat cgagttgcgc atcaacgccg aggacccgga
       61 ccacgacttc cggccgagcg cgggcctgat cacggacgtg cactggcctg gtgggcccgg
      121 catccgggtg gactcgcacg tgtactccgg ctatcgcatc ccgcccaact acgacagtct
      181 tatcgccaag atcatcgcat gggcgccgag tcgcgaggag gccatagccc ggatggagcg
      241 cgcgctcaag gagaccgtcg tggaaggcgt gaagaccacg atcccgttcc atctcaaggt
      301 gctcggcaac gccttcttca ggcgcggcga cgtgtacacg aacttcatcg cccgtaggat
      361 ggggtcatga ccgaaggacc ggacctcgtc atcgccggcg acgtgctcct caacatcgcc
      421 cagttggcgc tcgaccgggt cgagggggtg cgcccgaccc agccgccctc cagcgtctcc
      481 gacctcctcg gtggtaggcg cgcccgcggc atagccatcg agcgtgatgg ggaggacgta
      541 tgggtcgacc tgaccttggc ggtcgagtgc ggggtcgaga tcccgaaggt cgcgaaggcg
      601 gcgcagcggg ccgtgcgcga gggcatgacc tccatgacgg gcttgcacgt gcggagcgtg
      661 aacgtcgtga tcgagtccgt cgagcccagc caggcgtccg ttggggccgg tgccgcgacc
      721 gacgcggagg cggcgaccag cgccgagtcg gcgaccggcg cggccgggca agcggcgcag
      781 gaccgcgacc cgtgggagcg cgagacggcg cgcgagggcg ggccggcgac tagtgagtag
      841 acaacgtgcg cgggagctcg ccttccgcac cctcttccag tcggagcgtg gcgccgagcc
      901 gctcctggag gtgtggcgca acgtgcgcgt cgagctggcc gaggaggcgg cagagtcgcc
      961 ggaggacgcc gtttatggcg gtgcgctcgg gctggaggac ttggcgttcg ccgaggggct
     1021 gttgcaggcc ttcgacgagc accgcgagga gctggacgcc cgcctcgcga ccgccataac
     1081 cggttggtcg ttcggtcaga tggcccagac cgacctgaac gtgctgcgca tcgccagcgc
     1141 cgagatgcgc tacggctcgg aagcgccgga ggtggccatc gagatggccg tgcggctcgc
     1201 caagaagttc ggtggcgacg agtcgggcag gttcgtgaac ggcgttctgg ccaagctcat
     1261 ccgggacggc gaggggcgca ttccggccgc cgtgagcgcg gag
//
LOCUS       DAOQYE010000768         1301 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_134786_length_1301_cov_2.178170, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000768 DAOQYE010000000
VERSION     DAOQYE010000768.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1301
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134786_length_1301_cov_2.178170"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1301)
                     /locus_tag="PKN52_10970"
     CDS             complement(<1..>1301)
                     /locus_tag="PKN52_10970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00518.1"
                     /translation="EEVELTKLVGDAPLVLHHGSNARFITSALMYTDDPPSLPGYLRQ
                     IERWNGGGIENGLKRLRRTEWRGLQANVRFTVLAAHFENLFGLALLLLMLPLAAGLNY
                     ALPGHGTPLKALGIWAAIDLAATLVLATWGFARVWRAQGLRGFGLLSKVLRETATSVV
                     PLSLLRPFNAVTFLTGAARAVPRFARRRDVDPRATITWDRPRSRTSKRSQLRYVGATT
                     GMVLSVATVFSGAAVLASATRPGYRDAWRLINESVRVSQDDHMTLPLSLTDRGLLDAF
                     VDAGFSRPAQAADPGAGGASEGLAPGADAAGPVDAPTEFGDDARVAEAQGDDPGARAD
                     DPGVPTDLVDSPLARLGLHVMASNEGHGRVSAYCSVADVLRPATKRHRLEVAEGYQPL
                     SAWGLLVLARLAPLVANIEEASTAYDVPPDLLLRILINESY"
BASE COUNT          205 a          441 c          498 g          157 t
ORIGIN      
        1 ggtaggactc gttgatgagg atgcgcagga ggaggtcggg cggcacgtcg tacgcggtcg
       61 aggcctcctc gatgttggcc acgagcggcg ccaagcgcgc caggacgagc agaccccacg
      121 cggagagcgg ttggtagccc tccgccacct cgagccggtg gcgcttcgtg gccggccgca
      181 gcacgtcggc gacggagcag taggccgaca cgcggccgtg cccctcgttg gacgccatga
      241 cgtggagacc gagccgggcg agcggcgagt cgacgaggtc ggtgggaacg ccggggtcgt
      301 cggcccgcgc gccggggtcg tcgccctgcg cctcggcgac gcgcgcgtcg tcgccgaact
      361 cggtcggcgc gtccaccgga cccgcggcgt ccgcccctgg cgccaggccc tccgaggctc
      421 caccggcgcc cggatcggca gcctgcgcgg ggcgcgagaa cccggcgtcg acgaaggcgt
      481 cgagcagccc acggtcggtc aaggacagcg gcaacgtcat gtggtcgtcc tgcgacaccc
      541 tgaccgactc gttgatgagg cgccaggcgt cgcggtagcc ggggcgcgtg gcggaggcga
      601 gcacggcggc gcccgagaac accgtggcga cggacagcac catgcccgtg gtggcgccga
      661 cgtaccggag ctgcgagcgc ttcgaggtgc gggaccgcgg ccggtcccac gtgatggtgg
      721 cgcgcggatc gacgtcgcga cgcctggcga aacgcggtac cgccctggcg gccccggtca
      781 ggaacgtgac cgcgttgaag gggcgcaaca gcgagagggg taccacggac gtagccgtct
      841 cccgcagcac cttggagagc aacccgaagc cgcgcagacc ttgggcgcgc cagacgcgag
      901 cgaagcccca agtggcgagg acgagagtgg cggccaggtc gatggccgcc cagataccga
      961 gcgccttgag cggcgtgccg tgcccgggca gcgcgtagtt gaggccggcc gccagcggga
     1021 gcatgagcag cagcagcgcc agaccgaaca ggttctcgaa gtgcgccgcc agtacggtga
     1081 agcgcacgtt ggcctgcaac ccccgccact ccgtgcgccg gaggcgcttg aggccgttct
     1141 cgataccgcc gccgttccag cgctcgatct gacgcaggta gcccggcaag ctgggcggat
     1201 cgtccgtgta catgagagcg gacgtgatga agcgggcgtt gctgccgtgg tgcaggacga
     1261 gcggagcgtc accgaccagc ttggttagct ccacctcctc c
//
LOCUS       DAOQYE010000769         1297 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_135473_length_1297_cov_2.318035, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000769 DAOQYE010000000
VERSION     DAOQYE010000769.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1297
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135473_length_1297_cov_2.318035"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            25..>1297
                     /locus_tag="PKN52_10975"
     CDS             25..>1297
                     /locus_tag="PKN52_10975"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNR00519.1"
                     /translation="MACAVTMQADMARFASLRVGGGADGPTVALDVKVAVSRGTVRRF
                     AVGDETVQLLDVIAGDPVAELEALNNASRKGQIVLSAAVAAAVDGGYRLRPLEDAAAP
                     AGAMVVSLTDGSPAERRERGPVEAHAGAGGAGSLGGRGHEPHRHEPAPPDLPERWFAA
                     PEAAELAPAQQLLGRWLLPTVKMRLSAGGGDFLTELRPVAALFLAFDGLDLTGDEGAG
                     TKLDRLVRLVQQVVMEYGGNVLQLTTGDKGTYLYAAFGAPVSHEDDVSRCAAAALDLR
                     EAVLGLPFVAELKIGLGHGTARTGAYGSITRRTYGALGRGTNMAARMMSAAPHAAIYV
                     SGAFEKALGGGFDLRRLEDVVVKGSAAPVEAFELVGRASGTPRERTGADGRPLVGREP
                     ELEAISAGIAEAAAGRGAVIQVVAEAGMGKSE"
BASE COUNT          168 a          436 c          511 g          182 t
ORIGIN      
        1 ccggcggacg acggccgctc cgccgtggcg tgcgccgtaa ccatgcaggc cgacatggcg
       61 cggttcgcct ccttgcgcgt cgggggcggg gcggatgggc cgacggtcgc cctggacgtg
      121 aaggtcgcgg tcagccgcgg aaccgtgcgg cgcttcgcgg tcggcgacga gactgtgcag
      181 ttgcttgacg tcatcgccgg tgaccccgtg gcggagctcg aggcgctcaa caacgcctcg
      241 cgcaaggggc agatcgtgct cagcgccgcg gtggcggcgg ctgtggacgg cggttaccgg
      301 ctccgtccgc tcgaggacgc ggcggcgccg gccggggcga tggtcgtgag cctcaccgac
      361 gggtctcctg cggaacggcg agagcgcggc cccgtggagg cccatgccgg tgcgggcggc
      421 gcgggtagcc tcggcggacg cggacatgag ccgcataggc acgagccggc gccgcccgat
      481 cttccggagc gctggttcgc cgcgcctgag gcggccgaac tcgcacctgc ccagcagctg
      541 ctcgggcgct ggctgctgcc gaccgtgaag atgcggctga gcgcaggcgg gggcgacttc
      601 ctgacggaac tccggccggt ggcggcgctg ttcctcgcct tcgacggcct cgacctgact
      661 ggcgacgagg gcgccggcac gaagctcgac cggctcgtgc gcctggtgca gcaggtcgtg
      721 atggagtatg gcggcaacgt cctgcaactg acgaccggcg acaaggggac gtacctctac
      781 gcggcgttcg gcgcccccgt ttcgcatgag gacgacgtgt cgcgctgcgc tgcggcggcc
      841 ctcgacctgc gcgaggcggt gctcggtctg ccgttcgtcg cagaactcaa gatcggcctc
      901 ggccatggca cggctcgcac cggcgcttac ggttcgatca cccgtcgaac gtacggagcg
      961 ctcggtcgcg gcaccaacat ggccgcccgc atgatgagcg cggctccgca tgccgccatc
     1021 tacgtgagcg gcgcgttcga gaaggcgctg ggcggcggtt tcgacctgcg gcggctcgaa
     1081 gacgtggtcg tcaagggcag cgccgcgccc gtggaggcgt tcgagttggt ggggcgggca
     1141 tccggcaccc cgcgcgagcg gaccggcgcg gacggtcgac cgctcgtggg tcgggaaccc
     1201 gagctcgagg ccatatcggc cggcatcgcc gaggcggccg ccgggcgcgg cgctgtgatc
     1261 caagtggtgg cggaggccgg catgggcaag tccgagt
//
LOCUS       DAOQYE010000770         1293 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_136205_length_1293_cov_2.392569, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000770 DAOQYE010000000
VERSION     DAOQYE010000770.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1293
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136205_length_1293_cov_2.392569"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..933
                     /locus_tag="PKN52_10980"
     CDS             <1..933
                     /locus_tag="PKN52_10980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00520.1"
                     /translation="PMTPGRPGAQDAHPEVPIMYSRKTHHYTKRPRTAWLMLSALALL
                     TGLAQAAADVRTEYSNGSLRAPEIYAGEGFHSIGLINESENSVVFSVYRLHDGTDLAA
                     FEKTDAALQSALQTDDSVPAMKAFMAQADALSGTVVAAHSETDMFVDLEEGTYVVSAN
                     LDGDDAGAPTYTSFMVTEGGDQGTAPEVANVVHFSDFAFDFPATVAAGKDLWEVTNTG
                     DQPHIAAFFKLQPGKTTADLEAFLGGAAGEETPPPFDTSVDVNVEALTPGVTVYLPLD
                     FTSGDWVAVCFVPDLNNPTMAHYMEGMVEEFVVN"
     gene            1081..>1293
                     /locus_tag="PKN52_10985"
     CDS             1081..>1293
                     /locus_tag="PKN52_10985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00521.1"
                     /translation="MTKVLERAPFSRRNRARGAYRTTRTRHADSTRTPSQPPQQDRLR
                     CALRQRLGPGLANLEVRLELLEAAMTG"
BASE COUNT          235 a          455 c          420 g          183 t
ORIGIN      
        1 cccatgacac cgggacgccc gggtgcgcaa gatgcgcatc cggaggtacc gatcatgtac
       61 agccgcaaga cacaccacta cacgaagcgc ccgcgaaccg cctggctcat gctcagcgcc
      121 ctggcccttc tcaccggcct cgcccaagcg gcggccgacg tgcgcaccga gtactcgaac
      181 ggcagcctgc gcgcgcccga gatatacgcc ggggagggtt tccactccat cgggttgatc
      241 aacgagagcg agaactccgt cgtcttcagc gtgtaccggt tgcacgacgg cacggacctg
      301 gcggcgttcg agaagacgga cgccgccttg cagtcggcgc tgcagaccga tgactccgtg
      361 ccggcgatga aggcgttcat ggcacaggcc gacgcgctga gcggcaccgt cgtggcggcg
      421 cattccgaaa ccgacatgtt cgtcgacctc gaagagggca cgtacgtggt gtcggccaac
      481 ctcgacggcg acgacgcggg cgcgccgacg tacacgtctt tcatggtcac ggagggcggc
      541 gatcagggga ccgcgccgga ggtggccaac gtcgtgcact tctccgactt cgcgttcgac
      601 ttccccgcca ccgtggcggc cggaaaggac ctatgggaag tgaccaacac gggtgaccag
      661 ccgcacatcg cggcgttctt caagctccag cctggcaaga cgacggccga tctcgaggcc
      721 ttcctcggcg gcgcggccgg cgaggagacg ccccctccgt tcgacaccag cgtggacgtc
      781 aacgtcgagg ctctcacgcc aggagtaacc gtctacctgc ccctagactt cacgagcgga
      841 gactgggtgg cggtctgttt cgttccagac ctgaacaacc cgaccatggc ccactacatg
      901 gaagggatgg tagaggagtt cgtcgtcaac tgaggacacc gtcgggcgcg gcggccgctc
      961 accgtccgcg gcgcggccgc gtcctaccgc ggtggagcta gccggaggcg agggcgcggc
     1021 gatcatccgc accctcgcct ctcatattgg aggtgtagcc taggcgccgc ttaaggagcg
     1081 gtgaccaagg tgttagaacg cgcgcccttc agccgccgaa accgcgctcg cggagcgtac
     1141 cggaccaccc ggacccgcca cgccgattcg accagaaccc ccagccagcc gccacagcag
     1201 gacaggctgc gctgcgccct gcgccagcgc ctcggcccgg ggctcgcgaa cctcgaggtc
     1261 aggctcgaac tcctcgaggc cgccatgacc gga
//
LOCUS       DAOQYE010000771         1291 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_136585_length_1291_cov_2.396440, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000771 DAOQYE010000000
VERSION     DAOQYE010000771.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1291
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136585_length_1291_cov_2.396440"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..737
                     /locus_tag="PKN52_10990"
     CDS             <1..737
                     /locus_tag="PKN52_10990"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNR00522.1"
                     /translation="LGSTLAQSLGVSVGDTVMLRDISGATAQFIVAGTFRVGNELIDA
                     VTAYMSLPNLQAYLGAEGRVSGYHLRLSDPTAAHRVGLTLADKYSLRPVSWESLFASL
                     ISQLRLQKAVIGVVVFLIVIVAAFGITNVLVLTVNEKTADIAILRALGASEGQVLRTF
                     TLQGFALGGLGTLFGVVLGLAVAAYFKFQPYPLPGDLYFITQLPIQFQAPDVVWVCAV
                     SLVTSVVAGLLPARRAARLDPVAVLR"
     gene            737..>1291
                     /locus_tag="PKN52_10995"
     CDS             737..>1291
                     /locus_tag="PKN52_10995"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HNR00523.1"
                     /translation="MNEGQADRSGGRRPPRPLTRDTAWDYLLDLLSRRMYTTVELTRK
                     LLRRGLDPDLADSLVTRLMELELVNDAVYADLYVSTRSGAKGRLGLRQELRQKGVAPE
                     IAEARLAELTPDDQLTAATALLRKNAWRYRQAEADATDRLAALSEGEIFGRHYVRRRR
                     RRFEAGAAVTQFSDLKAGDYIVHEV"
BASE COUNT          194 a          463 c          430 g          204 t
ORIGIN      
        1 tgctcggctc cacgctcgcg cagtccctcg gcgtgagcgt cggtgacacg gtgatgctgc
       61 gcgacatctc aggcgccacg gcgcagttca tcgtggcagg aaccttcagg gtgggcaacg
      121 agctcatcga cgccgtgacg gcttacatgt ccctgccgaa cctccaggcc tacctgggcg
      181 ccgaggggcg cgtcagcggc taccacctgc gcctgagcga ccccacggcc gcccaccgcg
      241 tcgggctgac cctcgccgac aagtactcgc tcaggcccgt aagctgggag agcctcttcg
      301 ccagcctcat ctcccagctg cggctgcaga aggccgtgat cggggtcgtc gtcttcctca
      361 tcgtgatcgt cgccgcgttc ggtatcacca acgtgctcgt gttgacggtc aacgagaaga
      421 cggccgacat agccatcctg cgcgcgctag gcgcttctga aggccaggtg ctccgcacgt
      481 tcacgctcca ggggttcgcg cttggcgggc tcggcacgct gttcggcgtc gtcctcgggc
      541 tcgccgtcgc cgcctacttc aagttccagc cgtaccccct gccgggcgat ctgtacttca
      601 tcacccagct cccgatccag ttccaggccc cggacgtcgt atgggtgtgc gcggtgtcgc
      661 tggttaccag cgtggtggcg ggcctgctac cggcccgccg ggcggcgcga ctcgaccccg
      721 tggcggtgct ccgctgatga acgaggggca ggccgacaga agcggtgggc gtaggccacc
      781 gaggcccctc acgcgcgaca ccgcgtggga ctacctcctc gacctcctca gtaggcgcat
      841 gtacacgacg gtcgagttga cgcggaagct gctgcggcgc gggctggacc cagacctcgc
      901 ggactcgctc gtcacccgac tgatggagct cgaactcgtc aacgacgccg tctacgccga
      961 cctgtatgtg agcacacgct cgggggccaa gggccgcctt gggctaaggc aggagctgag
     1021 gcagaagggc gttgctcccg agatcgccga ggcgcgcctc gccgagctca ctcccgacga
     1081 ccagctcacc gccgccacgg ccctgctccg caagaacgcg tggcgttacc ggcaggccga
     1141 ggcggacgcc acggaccggc tggccgccct gagcgagggc gagatcttcg gccggcacta
     1201 cgtgcggcgc cggcgccgcc gcttcgaggc gggggccgcg gtcacccagt tcagcgacct
     1261 gaaagcgggc gactacatcg tccacgaggt c
//
LOCUS       DAOQYE010000772         1290 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_136733_length_1290_cov_2.658300, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000772 DAOQYE010000000
VERSION     DAOQYE010000772.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1290
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136733_length_1290_cov_2.658300"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            528..>1290
                     /locus_tag="PKN52_11000"
     CDS             528..>1290
                     /locus_tag="PKN52_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNR00524.1"
                     /translation="MVEEPTRTPVGADLPSRYDPAALEARWAERWAREPFAADAASPK
                     PPFCIVIPPPNVTGNLHLGHAFDNTIIDVLIRFKRMQGFEALFQPGTDHAGISTQVQV
                     EKALRKEGTSRQELGREAFLERVWEWKERYGGIILEQLQRLGVSADWTRTRFTMDEGL
                     SRAVRRQFVELYHQGLVYRGERIVNWDPVSKTVLSDLEVDREDRPGKLYELAYALQAA
                     DGRGDDGEITIATVRPETIFADVAVALHPDDPRAAG"
BASE COUNT          192 a          442 c          474 g          182 t
ORIGIN      
        1 gctggttcga gcgttgacag cgaccgctgc ccatggtggg gtaggcgggg tggtggcgtg
       61 gtggctggtt cgagcgttga cagcgaccgc tgcccatggt ggggtaggcg gggtggtggc
      121 gtggtggctg gttcgagcgt tgacagcgac cgctgcccat ggtggggtag gcggggtggt
      181 gctatcatct gcgccagcgg gaagagtagg cgcccccgcc ccgccagaga gtccgtgccg
      241 gttccgaacc gggtgagagc gcggaccggg agcgagcgcc gaacgtcgcc cgccggacgg
      301 atcgccgtcc tggggtgctg ggtcgcgtag tcgcgcctta tccatacacc cccgaaccgc
      361 tgccgcgtca ggcgcagcgt cctgagagtg cccccgcagc gtcgggggaa gtgcggtggt
      421 accgcggggt cacgcacgtg cctcgtccgc aactggccgg acggggtgtt ttcatctccc
      481 ttgcccctcc ggcgggcgcg ccaagcgctc cagagacgga ggagtccatg gtcgaggaac
      541 cgacacgaac accggtgggc gccgacctgc cgagccgcta cgacccggcc gcgctggagg
      601 cgcgctgggc cgagcggtgg gcgcgcgagc cgttcgcggc cgacgccgcc agccccaagc
      661 cgcccttctg catcgtcatc ccgccgccca acgtcacggg caacctgcac ctcgggcacg
      721 ccttcgacaa cacgatcatc gacgtcctca tccgcttcaa gcgcatgcag ggcttcgagg
      781 cgctgttcca acccggcacc gatcacgcgg gcatcagcac gcaggtgcag gtcgagaagg
      841 cgctgcgcaa ggagggcacg agccgccagg agctcggccg cgaggcgttc ctcgagcgcg
      901 tgtgggagtg gaaggagcgc tacggcggga tcatcctcga gcagctccaa cgtctcggcg
      961 tcagcgccga ctggacgcgc acgcgcttca ccatggacga ggggctgtcg cgcgccgtgc
     1021 gccgccagtt cgtcgagctc taccaccagg ggctcgtgta ccgcggcgag cgcatcgtca
     1081 actgggaccc cgtcagcaag acggtgctct ccgatctcga ggtcgaccgc gaggaccggc
     1141 ctggcaagct gtacgagctg gcgtacgcgc tccaggcggc ggacggtcgg ggcgatgacg
     1201 gcgagataac gatcgcgacc gtgcggcccg agacgatctt cgccgacgtc gccgtcgccc
     1261 tccacccgga cgacccccgc gccgccggcc
//
LOCUS       DAOQYE010000773         1289 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_136958_length_1289_cov_2.210697, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000773 DAOQYE010000000
VERSION     DAOQYE010000773.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1289
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136958_length_1289_cov_2.210697"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1089
                     /locus_tag="PKN52_11005"
     CDS             <1..1089
                     /locus_tag="PKN52_11005"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNR00525.1"
                     /translation="FDFAPSAPYVRIDVREPARGANDGIDAVALSPHKLLGGPGSSGV
                     LLIHESLYKLRSPTTAGGGTVTFVNRDDQRYVADVETREDAGTPGIMQKLRAAMAFRV
                     KERVGEGVIAEREEAFIRRAIGRLAGNPKVEILGNLEAPRLAVVSFLLKPTGRYLHPR
                     FVIRLLSDLFGIQGRAGCSCAGPYGHDLLGIDEVTSNAYLGQIEAGYDGLKPGWCRLS
                     FHFLVDEPEFEFLLSAIEFLAEEGDAFLPLYDFDWHSGAWRYAAAGAPGHQAGAGGVD
                     HMMRVGRARVGEDGAGPGDGHAPGHAAKAFEPEAWAPVDVSENPREEDYAAYLAEARR
                     LAARLRVAPPTAPDRTTTADTGLARFQY"
BASE COUNT          179 a          460 c          448 g          202 t
ORIGIN      
        1 tttgacttcg cgcctagcgc cccctacgtc cgcatcgacg tgcgcgagcc cgctcgcggc
       61 gccaacgatg gcatcgacgc cgtggccctg agcccgcaca agctcctggg tgggccgggg
      121 tcgtcgggcg tcctcctcat ccacgaatcg ctatacaagc tccggagccc caccacggcg
      181 ggcggcggta ccgtcacgtt cgttaacagg gacgatcagc gttacgtggc ggacgtcgag
      241 acgcgcgagg acgccggcac ccccggaata atgcagaagc tccgcgccgc catggcgttc
      301 cgcgtgaagg agcgagtcgg ggagggagtc atcgccgagc gcgaggaggc cttcatccgg
      361 cgcgccatcg gtcggctcgc cggtaacccc aaggtcgaga tccttggcaa cctcgaggcg
      421 ccgcggctcg ccgtcgtgtc gttcctcttg aagccgaccg gccgttacct gcacccgcgc
      481 ttcgtgatca ggctcctctc cgacctgttc ggtatccagg ggcgagcggg ctgttcgtgc
      541 gccggaccgt acggccatga cctcctcggc atcgacgagg tgacttcgaa cgcctacctc
      601 ggtcagatcg aggcgggtta cgacggtctc aagcccggct ggtgtcgcct gagcttccat
      661 ttcctcgtcg acgagccgga gttcgagttc ctcctctccg ccatcgagtt cctggccgag
      721 gagggcgacg cgttcctgcc cctctacgac ttcgactggc acagcggcgc ctggcgctac
      781 gcggcggctg gggcgccagg ccatcaggcg ggcgcgggcg gggtcgacca catgatgcgg
      841 gtagggcggg cgcgcgtagg ggaggacgga gccgggccag gcgatgggca cgcccccggg
      901 cacgctgcga aggcgttcga gcccgaagcc tgggctccgg tggacgtctc cgagaacccg
      961 cgcgaagagg attacgccgc gtacctcgcc gaggcgcggc ggctggcggc gcggctacgc
     1021 gtcgcgccgc cgaccgcccc ggaccgtacg acgacggccg acaccgggct agcccggttc
     1081 cagtactgac ctgcgctgcc ggtcggtcgg gcgttgatgg cgcctgcggt ctttggaccc
     1141 tgccgccggc gccagaccct gccgccggcg tcgcacgctc cggcgctggt cgccgagcct
     1201 agtggcttca ggccggcgcc acgccccgat gctagctctt cgtaagggct gcggcctctc
     1261 gtcgtacgtt cacggccagc cacagcact
//
LOCUS       DAOQYE010000774         1286 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_137437_length_1286_cov_3.691308, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000774 DAOQYE010000000
VERSION     DAOQYE010000774.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1286
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_137437_length_1286_cov_3.691308"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..133)
                     /locus_tag="PKN52_11010"
     CDS             complement(<1..133)
                     /locus_tag="PKN52_11010"
                     /inference="COORDINATES: protein motif:HMM:NF012404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HNR00526.1"
                     /translation="MGRRRRAEIRPLEPDLVYSDTTVTALVNKLMRDGKKNTASRIFY
                     "
     gene            complement(137..535)
                     /gene="rpsL"
                     /locus_tag="PKN52_11015"
     CDS             complement(137..535)
                     /gene="rpsL"
                     /locus_tag="PKN52_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HNR00527.1"
                     /translation="MPTVNQLLRKGRRKIEKKSKTPALKGSPQRRGVCTAVKTQTPKK
                     PNSALRKIARVRLAGGQEVTAYIPGEKHNLQEHSVVLIRGGRVKDLPGVRYHIVRGTL
                     DAQGVGVGRYVQRNQSRSKYGTKRPKAGKK"
     gene            complement(717..>1286)
                     /locus_tag="PKN52_11020"
     CDS             complement(717..>1286)
                     /locus_tag="PKN52_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNR00528.1"
                     /translation="RHVNQVLDVSSSPWAPGGAGPGAALRLGERDDPTGGLLTPGLLA
                     AALEATEATLAGLAFVGLGCVEFFVAADGALYVNEVAPRPHNSGHLTIEACAVSQFEQ
                     QVRALCGLPLGSPEPVSPAAMANLLGDLWDRGEPDWSAALREPAVRLHLYGKAAARPG
                     RKMGHLTAVAPDASEAVARVLRARLALAN"
BASE COUNT          180 a          449 c          447 g          210 t
ORIGIN      
        1 cgtagaagat gcggctggcg gtgttcttct tgccgtcgcg catcagcttg ttgactaggg
       61 cggtcaccgt cgtgtcggag tagacaagat cgggttccag cgggcggatc tcggcgcgtc
      121 ttctgcgtcc catggcttac ttcttccctg ccttcggcct cttggtgccg tacttgctgc
      181 ggctctggtt gcgctggacg tagcggccga caccgacgcc ctgggcgtcg agcgtgccgc
      241 gcacgatgtg gtagcgcacg cccggcaggt ccttgacgcg gccgccgcgg atgagcacga
      301 ccgagtgctc ctgcaggttg tgcttctcgc ccgggatgta ggccgtgact tcctggccgc
      361 cggccagacg gacgcgcgcg atcttgcgca gcgccgagtt gggcttcttg ggcgtctggg
      421 tcttgacggc ggtgcaaacg ccacgccgtt gcggcgaccc cttgagtgcc ggggtcttgc
      481 tcttcttctc gatcttcctg cgccccttgc ggagcagctg gttaacggtc ggcagaacga
      541 tcactccctc gtgtggtctc gcagccgttg ggaacccgga tgcggcgata aaggcctcga
      601 actcagctag cctgacgtcg ttcgcggcct tcgcggcagc acacgacaaa ctcgccctag
      661 acccaacgct gcgggccgag ggcagacaaa cctaggcagt atagcccgta tgccggctaa
      721 ttggcaagcg ccagacgcgc acgcagcacg cgcgccaccg cctcggaggc gtcgggcgcc
      781 accgccgtca ggtggcccat cttgcgaccg ggcctggcgg ccgccttgcc gtagaggtgg
      841 aggcgcaccg ccggctcgcg cagggccgcc gaccagtcgg gctcgccccg gtcccacagg
      901 tcgcccaaca ggttggccat cgcggccgga ctgaccggct cggggctccc gagcggcagg
      961 ccgcataagg cccgcacctg ctgctcgaac tggctcacgg cgcaggcctc gatggtgaga
     1021 tgccccgagt tgtgaggccg cggagcgacc tcgttcacgt acagggcgcc atcggcggcc
     1081 acgaagaact cgacgcatcc gagcccgacg aaagcgagcc ccgccaacgt cgcctcggtc
     1141 gcctcgagcg cggcggccag caggccgggg gtcaggagcc cgccggtcgg gtcgtcccgc
     1201 tcgccgaggc gcagcgcagc cccggggccg gcgccccccg gcgcccaggg cgagctggag
     1261 acgtcgagga cctggttcac gtgtcg
//
LOCUS       DAOQYE010000775         1285 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_137705_length_1285_cov_2.208943, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000775 DAOQYE010000000
VERSION     DAOQYE010000775.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1285
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_137705_length_1285_cov_2.208943"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(411..>1285)
                     /locus_tag="PKN52_11025"
     CDS             complement(411..>1285)
                     /locus_tag="PKN52_11025"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HNR00529.1"
                     /translation="DLKVVLLGSAPLLVQRGLTESLAGRFETLRIPHWSFSEMSQAFG
                     FTLEQFLYFGGYPGAASLVNERERWRRYVLDSLIETTIARDVLLMTRVDKPALLRRLF
                     ELACSYSGQVLSYTKLLGQLQDAGNTTTLAHYLDLLSGAGLVLGLQKYAGDKARSRGS
                     SPKLQVLNTALLTVQSDLALEDYRQDGALWGRLVESAVGAHLANAALMGEVEVFYWRE
                     RNREVDFVVKAGRRLTAIEVKSGGTPSSLPGMKAFVDTFGASRTLLVGGDGIGLAEFL
                     EVPVRHWIDDRTPT"
BASE COUNT          210 a          428 c          418 g          229 t
ORIGIN      
        1 cgccctcctc gggatagggg cggccgcctg gcgcgcctgg caccggcccg acgacgcagg
       61 tccgctcgag cgccacttcg ctggtagcgc cgacgaacga gacgccgtcg ttctcacgcg
      121 agcgctcgcc gtcgcgggcg tgaccgccct cccgttgacc gggcttgcga cggtggccat
      181 cgccgtgggc ctcaggtggg acatcgcttt ggcggtgctg ctgttcgctc agctcgccat
      241 cctggtcgtg gcgtaccgcc gcgaggtcgt ccgcggctgg gcgattcgta gagtgaaccg
      301 accgatggtc agggcttctc ggccttaccc gaggccgggc cgtcggccgg gaaccgctca
      361 agcgtccaat gcgatggaca gagtcaggtc ggccatccat catgtctcgt ctaagtgggc
      421 gttcgatcgt cgatccaatg ccgtaccggg acctcgagga actcggcgag accgatgccg
      481 tcaccaccaa ccagtagtgt cctcgacgct ccgaaagtgt ctacgaaggc cttcatgccc
      541 ggcaaggacg atggtgtgcc gccgctcttc acctcgatgg cagtgagcct tcggccggcc
      601 ttgactacga aatcgacctc gcggttccgt tcccgccagt agaagacctc cacttcgccc
      661 atgagggcgg cgttcgctag gtgcgcgcct acggccgatt cgacgagccg cccccaaagc
      721 gctccgtcct gccgatagtc ctcgagggcg aggtcgctct gaacggtcag cagcgcggtg
      781 ttcagcactt ggagcttcgg gctcgaaccg cggctgcgag ccttgtcgcc cgcgtacttc
      841 tggagcccga ggactagccc ggcgccgctc agtaggtcca ggtagtgcgc gagagtcgtg
      901 gtgttgccgg cgtcctgcag ttgacccagc agcttcgtgt aggagagtac ctgcccggaa
      961 tacgagcacg ccagttcgaa cagtctccgc aggagcgcag gtttgtccac cctggtcatc
     1021 aggagcacgt cgcgcgcgat ggtcgtctcg atgagagagt ccaacacgta gcgcctccag
     1081 cgttcccgct cgttcactag cgacgctgct cctggataac cgccgaaata caggaactgt
     1141 tccagcgtga agccgaacgc ttgcgacatc tcgctgaacg accagtgggg tatgcgtaac
     1201 gtttcgaagc gcccggcgag gctctcggtg agcccgcgct ggacaaggag gggcgccgag
     1261 ccgagcagca cgaccttgag gtccc
//
LOCUS       DAOQYE010000776         1283 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_138000_length_1283_cov_2.673453, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000776 DAOQYE010000000
VERSION     DAOQYE010000776.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1283
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138000_length_1283_cov_2.673453"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1283
                     /locus_tag="PKN52_11030"
     CDS             <1..>1283
                     /locus_tag="PKN52_11030"
                     /inference="COORDINATES: protein motif:HMM:NF023809.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3656 domain-containing protein"
                     /protein_id="HNR00530.1"
                     /translation="LKIEGRYKEADYVALTTAAYREALDAYAAARPPRIDADDERAIE
                     QVYSRWLGPWFMQGTDHQRVVVGRAPRHRGVRLGEVVAVESDGVRVRLAEPIAVKPGD
                     GVVFDAAHRRSPEEREEGGPIVRAEPNRVGEVRLTFLRGSVDLTRVAPGDWLWRTHDA
                     DVDAKARRYLTAAEPLHTRPLDLDVSATVDAPLIVTGRASGPRGAVSATVMGETALIA
                     ATRHGLGREQAVRQLGRLGGTPYHLGELRLETEGEPFVPVSELNRLRRELVERLQVAR
                     QAVGKGTTHDTRAPATTGHVPATGGARPATHVAAEGRLHLLVRTPEQLDAVLAIVADR
                     SSKTSIASVTLDYLELYGLRPSVERAHDAGIAVRVASPRILKPTEQNVVRFLLGLGAG
                     ILVRSGGLLHDLARLSAAERPELHGDFSLNVANAS"
BASE COUNT          189 a          476 c          457 g          161 t
ORIGIN      
        1 gcctgaagat cgaggggcgc tacaaggaag ccgattacgt cgcgctcacc acggccgcct
       61 accgggaggc cctcgacgca tacgctgccg cccgcccgcc gcggatcgac gccgacgacg
      121 agcgcgctat cgagcaggtc tactcccgct ggttgggccc atggttcatg cagggcacgg
      181 accaccagcg ggtcgtagtg gggcgcgcgc cacgccatcg cggagtcagg ctcggcgagg
      241 tcgtggcggt ggagtccgac ggcgtgaggg tccggctcgc cgagccgatc gcggtcaagc
      301 cgggcgacgg cgtggtcttc gacgccgccc accgccgcag ccccgaggag cgcgaggagg
      361 gcgggcccat cgtgcgtgcc gagccgaacc gcgtcggcga ggtgaggctg acgttcctcc
      421 gcggcagcgt cgatctgacc cgtgtggcgc cgggcgactg gctctggcgc acccacgatg
      481 ccgatgtcga cgccaaggcc aggcgctacc tcaccgcggc ggagcctctt cacacgcgtc
      541 cgctcgacct ggacgtgagc gccactgtcg acgcgccgct gatcgtcact gggcgcgcct
      601 cgggcccgcg cggagccgtg agcgccaccg tcatgggcga gaccgcgctg atcgcggcga
      661 cgcgccacgg cttggggcgc gagcaggccg tgcggcagct cggacgcctg ggaggcacgc
      721 cgtaccacct cggcgagctg cgcctcgaga ccgaaggcga gccgttcgtg ccggtcagcg
      781 aactgaaccg gttgcggcgg gagctcgtgg agcggctgca ggtggcgcgg caggccgtgg
      841 gtaagggcac gacccatgac acccgcgccc ccgcgacgac cggccacgtg ccggcgacgg
      901 gcggagcaag acccgccacc cacgtcgccg cggagggcag gctccacctc ctggtgcgca
      961 cccccgagca gctcgacgcc gtccttgcaa tcgtcgccga tcgctcctcc aagacgagca
     1021 tcgcgagcgt caccctcgac tacctcgagc tgtacgggct gagaccgtcc gtcgagcgcg
     1081 cgcacgacgc cgggatcgcg gtgcgggtcg cctcgccgcg catcctgaag cccaccgaac
     1141 agaacgtcgt caggttcctc ctcgggctag gcgccggcat cctcgtgcgt tcgggcggcc
     1201 tgctgcatga cctcgcacgc ctgagcgccg ccgagcgccc cgaactgcac ggcgacttca
     1261 gtctcaacgt cgccaacgcc agc
//
LOCUS       DAOQYE010000777         1281 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_138351_length_1281_cov_3.066069, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000777 DAOQYE010000000
VERSION     DAOQYE010000777.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1281
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138351_length_1281_cov_3.066069"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1281)
                     /locus_tag="PKN52_11035"
     CDS             complement(<1..>1281)
                     /locus_tag="PKN52_11035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00531.1"
                     /translation="HTGSVAQTGNRPATGRGGAPGTYVGRVGDPMNPSVYDEYALSKI
                     AGERRVMESQLERWVSLRMSFIMPTDHADLMGLFDPIAFHMPLDTRMENVTDRDAGLA
                     MVNCLELRQDAAFWRRAYNVGGGPGMRTTARAYLRAAYGLMGLDVRRCMDENWFGLRN
                     FHLQYYADSEVTERYLRYQRDDLASHAAALEATMAPAMRLLTWLCRHVAPVRRLSEAV
                     VHGRFLRLAHDHRNSPRRWYLTRNDARVRAFFGGYAAYEAIPAGGLSGAVDEDGPWVS
                     FDHGYPDDAESLSREHVMRAAALRGGAWLGTVAVEGTPRHPGVPGGNGEVADQGSAGD
                     GATVGWYEPSLWRCGAGHEFVALPSTVLRGGHWCADCLAQWDGGLRATREPFFAQAWY
                     ADHDPADLEPYPPEGARDVADADVVWRTRGGARR"
BASE COUNT          189 a          459 c          447 g          186 t
ORIGIN      
        1 ggcgccgtgc gccaccgcgc gtgcgccaaa cgacgtccgc gtccgccaca tccctcgcgc
       61 cttcgggcgg gtacggctcc aggtccgcgg gatcgtgatc ggcataccag gcctgggcga
      121 agaacggttc gcgagtcgcg cgcaggccgc cgtcccactg agccagacag tccgcgcacc
      181 aatggccgcc gcgcagcacg gtgcttggga gcgccacgaa ctcgtgaccc gcgccgcagc
      241 gccatagcga gggttcatac cagccgacgg tagcgccgtc gcccgcgctc ccttgatccg
      301 cgacctcacc gttcccgccg ggaacgccag ggtggcgagg ggtgccttcg accgccaccg
      361 tcccgagcca cgcgcccccg cgaagagcgg ccgcgcgcat gacgtgctcg cggctcaggc
      421 tctcggcgtc atcgggataa ccgtgatcga agcttaccca ggggccgtcc tcgtcgacgg
      481 ccccgctcag tcctccggca gggatggcct cataggcggc gtagccgccg aagaacgccc
      541 gtacccgcgc gtcgttgcgc gtcaggtacc aacgtctggg gctgttgcgg tggtcgtgcg
      601 ccaagcgcag gaagcgccca tggaccaccg cctcgctcag gcgccgcacg ggcgcgacgt
      661 gcctgcagag ccaggtcagg agcctcatgg ccggcgccat ggtcgcctcc agtgcggcgg
      721 cgtggctggc caggtcgtcg cgctggtaac gcaggtagcg ctcagtcacc tccgagtcgg
      781 cgtagtactg caggtggaag ttcctcaagc cgaaccagtt ctcgtccatg cagcggcgga
      841 cgtcgaggcc catcagcccg taagcggcgc ggaggtaggc gcgcgcggtc gtccgcatgc
      901 ccggtccgcc cccaacgttg taggcgcggc gccagaacgc ggcgtcttgc cgtagttcca
      961 ggcagttgac catcgccagg cccgcatcgc ggtccgtcac gttctccatg cgcgtgtcca
     1021 gcggcatgtg gaaggcgatg gggtcgaaca ggcccatgag gtcggcgtgg tcggtcggca
     1081 tgatgaacga catgcgcagc gagacccagc gctctagctg cgactccatc acgcggcgct
     1141 cgcccgcgat cttcgagagg gcgtattcgt cgtacacgga cgggttcatg gggtcgccga
     1201 cccgaccgac gtaggtgccg ggcgcgccgc cgcgcccggt cgccgggcga ttgccggtct
     1261 gcgcgacgga gcccgtgtgc a
//
LOCUS       DAOQYE010000778         1280 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_138535_length_1280_cov_3.226122, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000778 DAOQYE010000000
VERSION     DAOQYE010000778.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1280
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138535_length_1280_cov_3.226122"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..827)
                     /locus_tag="PKN52_11040"
     CDS             complement(<1..827)
                     /locus_tag="PKN52_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00532.1"
                     /translation="MNERMALIVLALVTALAVVFAPWAAINRETGARSAVVLLPERYV
                     DFTGRTQPIELPQQGAVLVLTLVGLALVAGGGALPAKARRFAWLAGGLVLVVGTAWGM
                     GRLTGAVESARYDAFLGEVRGAIERPRPAYDVDKLEALLAAAEQPDSERNLTEGLAEA
                     DAGGLVVRRLPYTNAGVGFSAFLAIVTGAFALLFGARVFRGAGQVIDRLLRAAAVPAT
                     AILLALLAAAVVILALQGTPVGAGAQVGGAFNEFVGRLDTLWYAYYSMFHDSLGTVNG
                     "
     gene            complement(856..>1280)
                     /locus_tag="PKN52_11045"
     CDS             complement(856..>1280)
                     /locus_tag="PKN52_11045"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNR00533.1"
                     /translation="SPPMVIVDASAIIAIMFEEVEASGCMAALQKDASRLISAVNYVE
                     AGTVLAGRIKSADRGEAIADLDAFLFDFRIAIDPIDESLARAALKARLDYGKGFGTRG
                     GLNFGDSFAYALAKRHSAPLLYVGADFALTDVRCALVP"
BASE COUNT          220 a          441 c          445 g          174 t
ORIGIN      
        1 ccgttcacgg tgccgagcga atcgtggaac atggagtagt aggcgtacca gagcgtgtcg
       61 aggcgcccta cgaactcgtt gaaggcgccg cccacttggg cgccggcgcc gacgggcgtg
      121 ccctgtagtg caaggatgac gaccgcggca gctagcagcg ccagcaggat ggcggtggcc
      181 ggcacggccg ccgcgcgcag cagccggtct atcacttgtc cggcgccgcg gaagaccctg
      241 gcgccgaaga ggagcgcgaa ggcgcccgtt acgatggcca ggaacgcact gaagccaacg
      301 cctgcgttcg tgtacggcag gcgcctgacg acgagcccgc cggcgtcagc ctcggccagc
      361 ccctcggtaa ggttgcgttc gctgtcgggc tgctcggcgg cggcgagcag cgcctcgagc
      421 ttatcgacgt cgtaagcggg ccgcggtctc tcgatggcgc cgcgcacctc gccgaggaac
      481 gcgtcgtagc gggcgctctc gacggcgccg gtcaaccgcc ccatgcccca ggccgtgccg
      541 acgacgagca cgaggccgcc tgccaaccac gcgaagcgcc gcgccttcgc gggcagcgcg
      601 ccgcctcccg ccacgagcgc caggccgacg agcgtgagca cgaggaccgc gccttgctgc
      661 gggagttcga tgggttgggt gcggcccgtg aagtccacgt accgctcggg gagcaggacg
      721 acggcgctgc gcgcgccggt ctcgcggttg atggcggccc acggagcgaa gacgaccgcc
      781 agcgccgtca ccaacgccag cacgatcagt gccatacgct cgttcacgct agccatcata
      841 cgtagcgccg gaccactacg gcaccagtgc gcacctgacg tctgtcagcg cgaagtcggc
      901 gccaacgtag aggagcgggg ccgaatggcg tttggcgagg gcgtaggcga agctatcgcc
      961 gaagttcagg ccaccgcgcg tcccgaagcc cttgccgtag tccaggcgcg ccttcaacgc
     1021 ggcacgcgcc agactctcgt cgatcgggtc gatggcgatg cggaagtcga acaggaacgc
     1081 atccagatcg gcgatagctt cgccacggtc agcgctcttg atcctgcccg ccagaacggt
     1141 cccggcctcg acatagttca ccgcggatat gaggcgcgaa gcgtccttct ggagggcggc
     1201 catgcagccg gacgcctcca cctcctcgaa catgatcgcg atgatggcgg acgcatcgac
     1261 gatcaccatc gggggtgatc
//
LOCUS       DAOQYE010000779         1280 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_138559_length_1280_cov_2.671837, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000779 DAOQYE010000000
VERSION     DAOQYE010000779.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1280
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138559_length_1280_cov_2.671837"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(33..977)
                     /locus_tag="PKN52_11050"
     CDS             complement(33..977)
                     /locus_tag="PKN52_11050"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNR00534.1"
                     /translation="MRVALEAAFRRSVLVLLMASAWLAAAHAQGVTVAHERGELTFDA
                     VPTRVVALEFSFVDALLNMGVTPVGVARDANPLPIIDALTEGVPSVGTRAAPNLEAIV
                     ALAPDLIIADLTRHGDLYDQLSAIAPTLLFNSLRGSYEDVLTQYVEIGRVFGRGAQAE
                     ADVAAHRAAYAEAAASAADSGKFVAAVDFANGFTAHSNASFVGSLLEMVGRTNALEPN
                     GGETQFSLSLDGLAAIDPEAIVVFRYAHETTSTDEWHESPVWQSLKAVQSGRVYVFDR
                     DNWTSARGLMALDAILTEMTDSGFLTDQPAAPGFGPAW"
BASE COUNT          196 a          385 c          475 g          224 t
ORIGIN      
        1 ggggcatggt cgtagtcatc ctggtcagtc gatcaccaag cggggccgaa gcccggggcg
       61 gccggctggt cggtgaggaa gccgctgtct gtcatctcgg tgaggatggc gtcgagggcc
      121 atcaggccgc gggcgctcgt ccagttgtcg cggtcgaaga cgtagacgcg gccggactgc
      181 acagccttga ggctctgcca gacggggctc tcatgccact cgtccgtcga ggtcgtctcg
      241 tgcgcgtagc ggaagaccac gatggcctcc gggtcgatgg cggcgagccc gtccaggctc
      301 aacgagaact gcgtctcgcc cccgttgggc tcgagggcgt tggtcctgcc gaccatctcg
      361 aggaggctgc cgacgaagct ggcgttgcta tgcgccgtga agccgttcgc gaagtcgacg
      421 gccgcgacga acttcccgga gtccgccgcg ctggcggcgg cctcggcgta ggcggcccta
      481 tgggccgcga cgtcggcctc ggcctgcgca cccctgccga agacgcggcc gatctcgacg
      541 tactgcgtca ggacatcctc gtagctgccg cgcaggctgt tgaagagcaa ggtcggagcg
      601 atggcggaga gttggtcgta caagtcgccg tggcgggtga ggtcggcgat gatcaggtcc
      661 ggcgcgaggg cgacgatggc ctcgaggttc ggcgccgcgc gggtgccgac ggacggcacg
      721 ccctcggtga gcgcgtcgat gatgggcagc gggttggcgt cgcgggccac cccgacgggc
      781 gtaactccca tgttcagcag ggcgtcgacg aagctgaact cgagcgccac gacacgggtc
      841 ggcaccgcgt cgaaggtgag ttcgccccgc tcgtgggcca ccgtcacgcc ctgggcgtgg
      901 gcggcggcga gccaggctga cgccatgagg agcactagga cggagcggcg gaacgcggct
      961 tcgagggcta cgcgcatctg gtttcctctg ttctgggcag cagcggctgc cggggatgat
     1021 tggtcgaacg gaaggcgagc ccgccattcc atagcatctt actcaggatt agtggtcgcg
     1081 caagcgcata cgtacagcca ccgcacgaac gggctagggc gttttcgcac tacgttgggt
     1141 ggtacttctg atgggtaggg caggggtcgg gggtcggggt gcttcttggg aagtgggtac
     1201 ttgtgcaact agctgattgc gcaactgcct cgttgcatat cgtgtcgttt ttcaaactag
     1261 acctcccatg ctcgccgtgc
//
LOCUS       DAOQYE010000780         1279 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_138694_length_1279_cov_4.366830, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000780 DAOQYE010000000
VERSION     DAOQYE010000780.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1279
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138694_length_1279_cov_4.366830"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="PKN52_11055"
     CDS             <1..350
                     /locus_tag="PKN52_11055"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178496.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the B subunit is part of the
                     catalytic core of the ATP synthase complex; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HNR00535.1"
                     /translation="GIGEGKTRKDHNNVADQLQAAYANGLDLRRLVAITGEDALSEND
                     KLYLKFADEFERHFVNQGSADRSVEESLSIAWAILSQLPKSELTRLSNDQIDKYYGAK
                     MDELWGAIRQDRL"
     gene            368..1000
                     /locus_tag="PKN52_11060"
     CDS             368..1000
                     /locus_tag="PKN52_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HNR00536.1"
                     /translation="MAENIAPTRSNLLQRRDQVKLAIRGADLLKRKRDALVGEFFALV
                     KSSLEARRQLSQASRDAYFALFLANAWDGPEAVGSLALAQDRTLEIGLKMENIFGVKV
                     PQVQPPTFDDALPFSPVGAGASTLEAARQFRKLVEAIAKVAATETRLRRIGEEIKKTN
                     RRVNALEQLVIPGINYEIRSIRSVLDQRALEEVTTLKRIKAKLEAREEAG"
BASE COUNT          230 a          449 c          425 g          175 t
ORIGIN      
        1 acggcatcgg cgagggcaag acgcgcaagg accacaataa cgtggccgac cagctgcagg
       61 ccgcgtacgc caacggcctc gacctcaggc gcctcgtcgc catcacgggc gaggacgccc
      121 tctccgagaa cgacaagctc tacctcaagt tcgcggacga gttcgagcgc cacttcgtga
      181 accagggcag cgccgaccgc tccgtcgagg agtcgctgtc gatcgcgtgg gccatcctgt
      241 cgcagctgcc gaagagcgag ctcaccaggc tctccaacga ccagatcgac aagtactacg
      301 gcgcgaagat ggacgagctc tggggcgcca tccgccagga ccggctgtag agcccgggag
      361 gtaggccatg gcagagaaca tcgccccgac cagatcgaac ctcctgcaga ggcgcgacca
      421 ggtcaagctc gccatccgcg gcgccgacct gctcaagcgc aaacgggacg cgctggtcgg
      481 cgagttcttc gcgctcgtca agtcgtcgct cgaggcgcgc cgccaactct cgcaggcgag
      541 ccgcgacgcg tacttcgccc tcttcctcgc gaacgcctgg gacggccccg aggccgttgg
      601 cagcctcgcg ctcgcccagg accggaccct ggagatcggg ctgaagatgg agaacatctt
      661 cggcgtcaag gtcccgcagg tgcagccgcc gacgttcgac gacgccctgc cgttctcgcc
      721 cgtcggcgcc ggcgcgtcca cgctggaggc cgccaggcag ttccgcaagc tcgtggaggc
      781 gatcgccaag gtggcggcca ccgagaccag gctgcggcgc atcggcgagg agatcaagaa
      841 gacgaaccgg cgcgtgaacg cgctggagca gctcgtcatc ccggggatca actacgagat
      901 caggagcatc cgctccgtcc tcgaccagcg ggccctcgag gaggtcacga cgctcaagcg
      961 catcaaggcc aagctcgagg cgcgcgagga ggccggctga ccgatccggt ccgcccgcac
     1021 gaccaacgct cgtcaggaag gcgcccccaa cgggcgcctt cctcatggtg cgggcccgct
     1081 tcgtccggtc acgccaggct tacgtcgtca ggcgccgcgc ccggcacggc gctcgctcga
     1141 tcggcggtcg agcatgccgt ggtagacggc gttacggtcc cggcaacgcg ttggcgcgtg
     1201 tgacgcgcgc tttgacaggg tagcggctta ccggataaag tcccgtagac ccctttctag
     1261 ctcccttgga ggggagtcg
//
LOCUS       DAOQYE010000781         1279 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_138791_length_1279_cov_2.387255, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000781 DAOQYE010000000
VERSION     DAOQYE010000781.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1279
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138791_length_1279_cov_2.387255"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="PKN52_11065"
     CDS             complement(<1..572)
                     /locus_tag="PKN52_11065"
                     /inference="COORDINATES: protein motif:HMM:NF019045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Na+/H+ antiporter"
                     /protein_id="HNR00537.1"
                     /translation="MTPATIELIAAALFGVAILHTFSAQVFSRLERSNPRHAGVWHLL
                     GEVEVVFGFWAMVLVVAFVATEGTKAATEYIDSRNFTEPMFVFAIMVIAGTRPIIQTA
                     MRLVRLLSRALPLPGSAGLYFVVLSVVPLLGSFITEPAAMTLAALVLANSLFARQISN
                     RLKYATLGVLFVNVSIGGTLTNFAAPPVLMV"
     gene            complement(710..>1279)
                     /locus_tag="PKN52_11070"
     CDS             complement(710..>1279)
                     /locus_tag="PKN52_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNR00538.1"
                     /translation="PVNQGGYLVMRRPWPGMLRTVYGDDDRYRNQYWGEVPHVYFSGD
                     GARRDADGYFWVMGRVDDVVNVSGHRLGTMEVESALVSHPAVAEAAVVGRPDELKGQG
                     IVAFVTLEKGFEATDKLREELRKHVAAEIGAIARPDEVRFADALPKTRSGKIMRRFLR
                     SVATGQELIGDTSTLEDRGVVDQLRQEGA"
BASE COUNT          217 a          416 c          453 g          193 t
ORIGIN      
        1 accatgagca ctggcggagc ggcgaagttg gtcagcgtgc cgccgatcga gacgttgacg
       61 aacagcacgc cgagggtggc gtacttgagg cggttcgaga tctgccgggc gaacaggctg
      121 ttggccagga ccagcgccgc caaggtcatg gcggctggct ccgtgatgaa ggagcccagc
      181 agcggtacca cggagagaac gacgaagtag aggccggcgc tgccgggtag cgggagcgct
      241 cgtgacagaa gccgtacgag acgcatggcc gtttggatga taggtcgcgt tccggcgatg
      301 accatgatcg cgaagacgaa catcggctct gtgaagttgc gtgagtctat gtactccgtc
      361 gcggccttgg ttccttcggt cgcgacgaac gccacgacga gcaccatcgc ccagaagccg
      421 aagaccacct cgacctcgcc gagcaggtgc cacaccccgg cgtgacgagg gttggagcgc
      481 tccaggcgcg agaacacctg agcggagaac gtgtgcagta tcgcgacacc gaacagagcg
      541 gcggcgatca gctcgatcgt ggcgggagtc atggctgagg gtccgtcacg gcggtcgacc
      601 ttaccatccg aggcatgggt ccaaggtcct cgcgcaacgc ggctgccgaa gccggggaga
      661 ctctagagat cgcctctcag gctccgggac cctcctgctc cgggggcgct tatgccccct
      721 cctgccgcaa ctggtccacc acgcccctgt cctccaacgt gctcgtgtcc ccgatcagtt
      781 cctggcccgt cgccacgctg cgcaggaagc gccgcatgat cttgccggag cgggtcttgg
      841 gcagggcgtc ggcgaagcgc acctcgtcgg ggcgggcgat ggcgccgatc tcggcggcca
      901 cgtgcttgcg gagctcctcg cggagcttgt cggtcgcctc gaagcccttc tcgagggtga
      961 cgaacgccac gatgccctgg cccttcagct cgtcggggcg gccgaccacc gcggcctccg
     1021 ccacggcggg gtgcgagacg agcgccgact cgacctccat ggtgccgagg cggtggccag
     1081 aaacgttcac gacgtcgtca acgcggccca tgacccagaa gtagccgtcg gcgtcgcggc
     1141 gcgccccgtc gccggagaag tagacgtgcg ggacctcgcc ccagtactgg ttgcggtagc
     1201 ggtcgtcgtc gccgtacacg gtgcgcagca tgccgggcca ggggcggcgc atgacgaggt
     1261 agccgccctg gttgacggg
//
LOCUS       DAOQYE010000782         1278 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_138998_length_1278_cov_2.265740, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000782 DAOQYE010000000
VERSION     DAOQYE010000782.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1278
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138998_length_1278_cov_2.265740"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            21..662
                     /locus_tag="PKN52_11075"
     CDS             21..662
                     /locus_tag="PKN52_11075"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNR00539.1"
                     /translation="MQDDPAPLNVVDLRVRLAGVNVLDDVTFRLEAGDVALLVGPNGA
                     GKSTLLRAVIGLLPSNGEIEIFGRPNRSMSARSKFVFSPDDPALYEDLTLREHARLNA
                     ILYGRPDSDARALEWLGAFGLEARLDEYPGTHSRGMRQKLALSLALGLELPLTILDEP
                     FNGLDIASQELLAAGLQARGAAGGAVLLTGHQRDLESVLEARRLELVDGALRA"
     gene            659..>1278
                     /locus_tag="PKN52_11080"
     CDS             659..>1278
                     /locus_tag="PKN52_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00540.1"
                     /translation="MTAGVTFLRRRTVGRALRAYREWVGRAVGWWTIVYMIAWIVVFV
                     IAGLETLTPLATPVSGARPYLVAAFVVLFASVILARRVPPVILDRRDLFRLGLAPVSP
                     WSALRWRFVLKVVGAAAVGVFVGLLWTALAPALFWQATPFAAPVVGLVLVARFCAAWL
                     VYAYRAAGLRAWHVALLGIALSIVPYGFADAMPSSATCQARRPAAR"
BASE COUNT          144 a          467 c          438 g          229 t
ORIGIN      
        1 ccggttggta gagtgccgac atgcaagatg acccggcgcc gctcaacgtc gtggacctgc
       61 gcgtgcgctt ggcgggcgtc aacgtcctgg acgatgtcac gttccgactt gaagcaggcg
      121 acgtggcgct ccttgtcggc cccaacggtg ccggcaagag cacgttgctg cgcgccgtca
      181 tcggcctcct gccgagcaac ggcgagatcg agatcttcgg gcggccgaac cgcagcatga
      241 gcgcgcgctc gaagttcgtc ttctcgcccg acgacccagc gctgtacgag gacctcaccc
      301 tccgtgagca cgcccgtctc aacgccatcc tgtacggccg gcccgactcc gacgcccgcg
      361 cgctggagtg gttgggcgcg ttcggcctgg aggcgcgcct cgacgagtac cccggcacgc
      421 actcgcgcgg catgcgacag aagctcgcgc tctcgctcgc gctcgggttg gagctgcccc
      481 ttaccatcct cgacgagccg ttcaacggcc tcgacatcgc ctcgcaggag ctcctcgccg
      541 ccgggctgca ggctcgtggc gccgccgggg gcgccgtgtt gctcactggg caccagcgcg
      601 acctggagag cgtgctcgag gcgcgccgac tcgaactcgt cgacggcgcc ttgcgcgcgt
      661 gacggccggg gtcacgttcc tcaggcggcg cacggtgggc cgcgccttgc gggcctatcg
      721 cgagtgggtc ggccgggcgg tcggctggtg gacgatcgtc tacatgatcg cttggatcgt
      781 cgtcttcgtg atcgccggtc tcgagacgct cacgccgctc gccacacccg tctcgggcgc
      841 gcgaccgtac ttggtcgcgg ccttcgtcgt cctgttcgcg agcgtgatcc tggccaggcg
      901 ggtgccgccc gtcatcctcg accgccgcga cctgttccgc ctcggactcg ctcccgtgtc
      961 gccgtggtcc gcgctccggt ggcgcttcgt gctcaaggtc gtcggtgccg ccgccgtcgg
     1021 cgtcttcgtc ggtcttctgt ggacggccct cgcgcctgcg ctgttctggc aagccacccc
     1081 gttcgccgcc cccgtagtcg ggttggtgct ggtggcgcgt ttctgcgccg catggctcgt
     1141 gtacgcctac cgggccgcgg gcctgcgcgc ctggcacgtc gcactgcttg gcatcgcgct
     1201 gagtatcgtc ccttacgggt tcgccgacgc gatgccaagc agtgcgacgt gccaggcgcg
     1261 caggcccgcg gcccggta
//
LOCUS       DAOQYE010000783         1277 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_139111_length_1277_cov_3.256956, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000783 DAOQYE010000000
VERSION     DAOQYE010000783.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139111_length_1277_cov_3.256956"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1277
                     /locus_tag="PKN52_11085"
     CDS             <1..>1277
                     /locus_tag="PKN52_11085"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNR00541.1"
                     /translation="ARRFDWLLPWYYLLPLALFLAVGIGFLGAVLVRLVAPDAPAYVG
                     IPVAAVALVLILRYLARRFDWLLPWYYLLPAIVFLLTFTVFPVVLTVFLAFTDYAGIR
                     NGELNISSETSIVAVDGVNLTIADAATLDCVDLRDGCNGVRAVVYASGMETVAAESLD
                     GTRLVIAGDLPAGRDVIGVELDLPDLGFPVSLRVTGVDGNVLTLERAPPFPPDLSDVR
                     VELDRAAIARTIVSESGDRLTLDEPLPEGLEASAIARYNDFGFVGLRNFRRVIASAPR
                     ALIPVFLWNVSFGFLTVLLNTAVGVLIAVLLNNKALRFRNIYRTLLIVPWALPSVITI
                     QVWRGFLNQNFGAINRVLALLDVSPAPINWLAGDPMAARAAILVVNLWLGLPFIMTAT
                     LGALSAIPDEIYEAARIDGANVWQGFWNVTGPL"
BASE COUNT          164 a          494 c          383 g          236 t
ORIGIN      
        1 tggcgaggcg cttcgactgg ctcctcccct ggtactacct cctcccccta gcgctgttcc
       61 tggccgtcgg gatcggtttc ctcggcgccg tgctggtgag gctcgtcgcg ccggacgccc
      121 ccgcctacgt cggtatcccg gtcgcggctg tcgcgctcgt cctcatcctg cgctacctgg
      181 cgaggcgctt cgactggctc ctcccctggt actacctcct cccggcgatc gtcttcctgc
      241 tcaccttcac cgtcttcccc gtcgtcctga ccgtgttcct cgccttcacc gactacgcgg
      301 gcatccgtaa cggcgagctg aacatcagca gcgagacgtc gatcgtcgcc gtcgacggcg
      361 tgaacctgac gatcgccgac gccgcgaccc tggactgcgt cgacctgcgt gacggctgca
      421 acggcgttcg ggccgtcgtc tacgccagcg gcatggagac cgtggcggcc gagtcgctcg
      481 acggcacccg cctggtgata gcgggcgacc tcccggccgg cagggacgtc atcggcgtgg
      541 agctggacct gcccgacctc ggcttcccgg tcagcttgag ggtcaccggc gtcgacggca
      601 acgtgctcac cctggaacgg gcgccaccgt tccctcccga tctctccgac gtgcgcgtgg
      661 agctcgaccg cgccgccatc gccagaacga tcgtgtcgga gagcggcgac cgcctgacgc
      721 tcgacgagcc gctgccggag ggtctcgagg cctccgccat cgcgcgctac aacgacttcg
      781 ggttcgtggg cctgcgcaac ttcaggcgcg tcatcgcctc cgccccccgc gcgctcatcc
      841 ccgtgttcct gtggaacgta tctttcggct tcctcaccgt cctactcaac accgccgtcg
      901 gcgtgctcat cgccgtcctg ctcaacaaca aggccctgcg cttcaggaac atctaccgca
      961 cgctcctcat cgtcccgtgg gccctgccca gcgtcatcac catccaggtg tggcgcggct
     1021 tcctcaacca gaacttcggg gcgatcaacc gcgtcctcgc gctcctcgac gtctcgcccg
     1081 ctccgatcaa ctggctggcg ggcgatccga tggcggccag ggcggcgatc ctcgtggtca
     1141 acctgtggct cgggctcccg ttcatcatga ccgccaccct cggcgccctc tcggcgatcc
     1201 ccgacgagat ctacgaggcc gcgcgcatcg acggcgccaa cgtctggcag gggttctgga
     1261 acgtgaccgg gccgttg
//
LOCUS       DAOQYE010000784         1275 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_139539_length_1275_cov_2.609836, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000784 DAOQYE010000000
VERSION     DAOQYE010000784.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139539_length_1275_cov_2.609836"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="PKN52_11090"
     CDS             <1..437
                     /locus_tag="PKN52_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HNR00542.1"
                     /translation="FKGIANIVEATSKEEQIHGLFGYQLVRTLREENPEWFDAAFTER
                     VRAACLEAREAERGILDWLFEAGELDFLPRAVVDAYIEQRFDDALAAIDMPPLFEPDE
                     ALVAETRWFEEEVLAGKHYDFFHKRPTTYTKKAKSITSDDLF"
     gene            complement(975..>1275)
                     /locus_tag="PKN52_11095"
     CDS             complement(975..>1275)
                     /locus_tag="PKN52_11095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNR00543.1"
                     /translation="SGGQQQMVAIGRALMLHPKVLIMDEPSLGLAPLLVREVMHVIRN
                     VADTGMPILLVEQNVTQVLRISERAYVLENGRLALEGPSLQLRGDPMIRRAYLGL"
BASE COUNT          190 a          439 c          435 g          211 t
ORIGIN      
        1 tcttcaaggg gatcgcgaac atcgtcgagg cgacgagcaa ggaagagcag atccacggcc
       61 tcttcggcta ccagttggtc aggacgttgc gcgaggagaa cccggagtgg ttcgacgccg
      121 ccttcaccga gcgcgtgcgc gccgcctgcc tggaggcgcg cgaggccgag cgcggcatcc
      181 tcgactggct cttcgaggcc ggcgagctcg acttcctgcc gcgcgccgtc gtcgacgcct
      241 acatcgagca gcgcttcgac gacgccctcg ccgccatcga catgccgccc ctgttcgagc
      301 ccgacgaggc gctcgtggcc gagacgcgct ggttcgagga ggaagtgctg gcgggcaagc
      361 attacgactt cttccacaag cggccgacta cctacacgaa gaaggccaag tcgatcacca
      421 gcgacgacct gttctagccg cttctaaccg cgcgggtccg gctggtcgcg ctcgccctgc
      481 tagtcgcgct cgtcgtgcgc tcgtcgcgtt gtctggtggg ttgggcggcg gagtgcgccc
      541 ctacgtacct gcctgcgctt cgctccggca gtactccggg cacactccgc cgcccaaccc
      601 acctcgaccg tccttggcgg agccatctgg cgtccccggt tccacacttt tggcctagcg
      661 gtcgcccgcc cgatcggttg tcgtacccac ggtcacgtag gtgtccctgg cggtggaggg
      721 gtcgacgtgc gtacgcggca cacccgagcc ggactgggtg ccggtgctcg gggcactggc
      781 tagcgcgcgt ggcgtcggcg acgaactccc gccggagagc tccttcctgc ccacggcttc
      841 gccactacgt gtcgaggtgg gtggggcgag gaggtgcgcc cggagtactg ccgtagcgca
      901 gcgtaggcag gtacgtaggg gcgcacctcc tcgccccacc caccagacga cgagacgtag
      961 ctgacgagac gtagctacag cccgaggtac gcccgcctga tcatcgggtc gccgcgcagc
     1021 tgcagggatg gcccctccag cgccagccgg ccgttctcga gcacgtaggc ccgctcgctg
     1081 atcctcagga cctgcgtgac gttctgctcg acgaggagga tgggcatgcc cgtgtcggcc
     1141 acgttgcgga tgacgtgcat gacctcgcgc acgagtagtg gcgccaggcc gaggcttggc
     1201 tcgtccatga tgaggacctt cgggtggagc atcagggcgc ggccgatggc gaccatctgc
     1261 tgctggccac cggag
//
LOCUS       DAOQYE010000785         1275 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_139598_length_1275_cov_2.067213, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000785 DAOQYE010000000
VERSION     DAOQYE010000785.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139598_length_1275_cov_2.067213"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(511..825)
                     /locus_tag="PKN52_11100"
     CDS             complement(511..825)
                     /locus_tag="PKN52_11100"
                     /inference="COORDINATES: protein motif:HMM:NF024057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HNR00544.1"
                     /translation="MPGDFEQLTSEIRAFAAARDWHRYHAPKNLVMALSVEVAELMEH
                     FQWLTNEESDALGPVERAGVLDELADVQIYLLELADRLGVDMASAVRAKMVKNGVKYP
                     AP"
     gene            complement(996..>1275)
                     /locus_tag="PKN52_11105"
     CDS             complement(996..>1275)
                     /locus_tag="PKN52_11105"
                     /inference="COORDINATES: protein motif:HMM:NF013224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HNR00545.1"
                     /translation="ITLLSLQPGHVPAAAARLAEYPNVRFVGNSFGSADIIIQTLHPS
                     AQDLHAFIATTLPRDIPSITRTETFQLAEVVKSSWDWKAWFAGQAEEG"
BASE COUNT          224 a          431 c          403 g          217 t
ORIGIN      
        1 cgttcgaagg cagcacgaag atcgtgacct cactcgggac ggactcccgt agctgttcat
       61 cctcgactag tagcccgagg atcagcacga tccatgcctg ctggcgctcg atgagcgtca
      121 acttcttagg atctggaagg tcttgagcct tgatcacgtt cgctctcctg tctcgcaggc
      181 ccgagtttcg ctcgagcccg gcctgatgcg ccaggcgccc actcagcttc gagactggca
      241 gcgaagcgtc gagtctgtcg acttctgatc tccagaagca cccgtatccg gatgtcatga
      301 gcgacgataa caacgaagtc ctttgacttg tctataggcg gcaccgcgaa cctcccctca
      361 ggaaccaggc gctagggttc cataagggtg actgagccgc cagccgcgtg cactgcctcc
      421 atggatcacg gcccacttgg tgtaggcgaa actgcgcccg gagccaacgc gcgcacaggt
      481 gctaccaccg tcatgacaag agatgtgctg ctaaggcgcc gggtacttga ccccgttctt
      541 gaccatcttc gcgcgcacgg cggaggccat gtcgaccccc aaccggtccg ccagctcgag
      601 caggtagatc tgcacgtcgg cgagctcgtc aagcacgccg gcacgttcga cgggtcccag
      661 cgcgtccgac tcctcgttcg tgagccactg gaagtgctcc atcagctccg cgacctcgac
      721 ggacagggcc atgacgaggt tcttcggtgc gtggtagcgg tgccagtcgc gcgccgccgc
      781 gaacgcgcgg atctccgagg tgagctgttc aaagtcgccg ggcatattgg tcaggttagc
      841 gcgcgggctg cggctgcggc cgcacgaccg gagctcgggg gcgcgccaag gactcgggag
      901 cgggccgcac ccctagaccc acatgcgctc ggccatcacg cccactcagc cgccgaaacg
      961 caagcagcca ctagccacca acgccgcagc tcccctcacc cctcctccgc ctgccccgcg
     1021 aaccacgcct tccagtccca tgagctcttg accacctcgg cgagctggaa cgtctccgtg
     1081 cgcgtgatgg acgggatatc gcgcggcagc gtcgtggcta tgaaggcgtg caggtcctgg
     1141 gcgctcgggt ggagcgtctg gatgatgatg tcggccgagc cgaacgagtt gcccacgaag
     1201 cgcacgttcg ggtactcggc gaggcgcgcg gccgcggccg gcacgtggcc gggctggagg
     1261 ctcaggagcg tgatg
//
LOCUS       DAOQYE010000786         1273 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_139901_length_1273_cov_2.635468, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000786 DAOQYE010000000
VERSION     DAOQYE010000786.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1273
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139901_length_1273_cov_2.635468"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            5..>1273
                     /gene="gcvPA"
                     /locus_tag="PKN52_11110"
     CDS             5..>1273
                     /gene="gcvPA"
                     /locus_tag="PKN52_11110"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HNR00546.1"
                     /translation="MRYIPHTREDVERALAKVGAPSIDALFADLPKELLDPDIRVPSG
                     MDEAGLLAHLRALAAKNRPEGPNFLGGGARRHFTPSVTAHLAMQSEFVTAYTPYQPEV
                     AQGILQATFEYQTIMSELTGLPVSNASMYDGASAVAEGVLLAMRQTGRDRVLVSRGVH
                     PETRAVIATYLTALDLELELVDLDPHATPAASVTASMADDVACVVAQQPNYLGYIEDM
                     AGLADAAHASGALLVAAVDPLSLAVLTPPGEYGADIAAGDGQTLGNPLNFGGPAFGFM
                     VVGEELIRQFPGRLVGETVDVDGKRAFVLTLQAREQHIRRSKAKSNICSNHQLTAIMA
                     AINVAALGPAGLRDLAVGSVVKAHELADALERAGFAPRVEARFFNEFVLPVGQAPAKL
                     RSALARAGVHAGVPVPEEYGLGAAIVLSATE"
BASE COUNT          190 a          483 c          424 g          176 t
ORIGIN      
        1 ctgagtgcgt tacatcccgc acacgcggga ggacgtggag cgcgcgctgg ccaaggtcgg
       61 cgcgccttct atcgacgccc tcttcgcgga cctaccgaag gagctgctcg acccggacat
      121 ccgggtgccg agcggcatgg acgaggccgg tctcctggcc cacctgcgcg ccttggcggc
      181 caagaaccgc cctgagggcc ccaacttcct cggcggcggc gcacgccggc acttcactcc
      241 gtccgtgacg gcccacctcg cgatgcagag cgagttcgtg accgcctaca cgccgtacca
      301 gcccgaggtc gcccagggca tcctgcaagc cacgttcgag taccagacga tcatgtccga
      361 actgacgggc ctccccgtga gcaacgcgag catgtacgac ggcgccagcg cagtggccga
      421 gggcgtgctg ctagccatgc gccagacggg ccgcgaccgc gtgctcgtca gtcgcggcgt
      481 gcaccccgag acgcgcgcgg tcatcgccac ctacctgaca gccctcgacc tcgagctcga
      541 gctcgtcgac ctggacccgc acgcgacgcc agccgcgagc gtgacggcgt ccatggcgga
      601 cgacgtggca tgcgtggtcg cccagcagcc caactacctc ggctacatcg aggacatggc
      661 ggggttggcc gacgccgccc acgcttcggg cgccctcctg gtggcggccg tcgaccccct
      721 gagcctcgcg gtcctcaccc ctccgggcga gtacggggcc gacatcgcgg ccggcgacgg
      781 ccagacgctc ggcaaccccc tcaacttcgg cggtccggcg ttcggcttca tggtcgtcgg
      841 cgaggagctc atccgccagt tccctgggcg cctcgtcggc gagacggtcg acgtggacgg
      901 caaacgggcg ttcgtcctca cgttgcaggc ccgcgagcag cacatccgca ggtcgaaggc
      961 gaaatcgaac atctgctcca accaccagtt gacggccatc atggccgcca tcaacgtcgc
     1021 cgccctcggc cctgccggcc tgcgcgacct cgcggtcggc tccgtggtca aggcccacga
     1081 actggccgac gcgctagaac gcgccggctt cgcgccgcgc gtcgaggcgc ggttcttcaa
     1141 cgagttcgtc ctgccggtcg gccaggcgcc agccaagctc aggtcggcgc tggcgcgagc
     1201 cggcgtgcac gccggggtgc cggtgccgga ggagtacggc ctcggcgccg cgatcgtcct
     1261 cagcgccacc gag
//
LOCUS       DAOQYE010000787         1273 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_139918_length_1273_cov_2.416256, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000787 DAOQYE010000000
VERSION     DAOQYE010000787.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1273
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139918_length_1273_cov_2.416256"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="PKN52_11115"
     CDS             <1..286
                     /locus_tag="PKN52_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNR00547.1"
                     /translation="LAKSTVILRHALRNALNPVITVVGLQFGALLGGAVIIEQVFALP
                     GVGRFALEGINLRDYPVVQGSVLLISVAFIAVNVLVDTVYSLVDPRIRYA"
     gene            279..1115
                     /locus_tag="PKN52_11120"
     CDS             279..1115
                     /locus_tag="PKN52_11120"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNR00548.1"
                     /translation="MPKAQGFARLWRSGAGVTGALIVLVVTLAAVLANSLTPYDPITL
                     NAPDKLQGPTVSHLLGTDQYGRDTMTRVLHAGRVSLGVALASVAFSLLIGAVLGVLAG
                     YYRGIVEVVIMRVTDVLLSFPAILLAIALLAFLGGGFRNLVLAIGIVYIGPFARVTRS
                     AVLSVRQELYVESSVAMGSRDWRTMFVTVLPNAIAPLIVETTLRLAYAILAEASLSFL
                     GLGAQPPAASWGQMIAEGRRYLSLNPWPTISPGFAIMLAVLGLNLLGDGLRDALDPRV
                     RD"
BASE COUNT          162 a          439 c          422 g          250 t
ORIGIN      
        1 gctcgccaag agcaccgtca tcctgcgcca cgccctgcgc aacgccctga accccgtgat
       61 cacggtggtc ggcctgcagt tcggggcact cctaggcggt gctgtcatca tcgagcaggt
      121 cttcgcgctg cccggggtgg ggcgcttcgc tctggagggc atcaacctgc gcgactaccc
      181 ggtggtgcaa ggctcggtcc tgctgatctc cgtggccttc atcgccgtca acgtgctggt
      241 cgacaccgtc tattcgctgg tcgacccgcg gatccgctat gcctaaggcg caggggttcg
      301 cgcgcctgtg gcggtcgggc gccggtgtca ctggggcgct catcgtcttg gtcgtgacgt
      361 tggcggccgt actcgccaac agcctcacgc cgtacgatcc gataaccctc aacgcgccgg
      421 acaagttgca gggccccacc gtctcccacc tgctcggtac cgaccagtac ggccgcgaca
      481 ccatgacgcg cgtgctgcac gctgggcgcg tttcgttggg agtggcgttg gcgtcggtgg
      541 cgttctcgct actcatcggc gcggtgctcg gcgtgttggc cgggtattac cggggcatcg
      601 tcgaggtcgt catcatgcgc gtcaccgacg tgctcctgtc gttcccggcg atcctgctgg
      661 ccatcgccct actcgctttc ctcggcggcg gtttccgcaa cctcgtgctt gcgataggca
      721 tcgtctacat cgggccgttc gcgcgcgtca cgcggtcggc ggttctctcc gtgcgccagg
      781 aactctacgt ggagtcgagc gtggcgatgg gcagccgcga ttggcgcacc atgttcgtga
      841 cggttctacc caacgccatc gcaccgctca tcgtcgagac gaccctacgc ctggcctacg
      901 ccatcctcgc cgaggcgtca ctgtccttcc tgggcctcgg agcccagccg cccgccgcca
      961 gttgggggca gatgatagct gagggacggc gttacctgtc cctcaacccc tggcctacga
     1021 tctcgccggg ctttgctatc atgctcgcgg ttctcgggtt gaacttgctc ggcgacggat
     1081 tgcgcgatgc gttggacccg cgtgtgcgtg actgacgtgg agccggctcg gcggcggatc
     1141 gtgaggcgcc tcggatctgc gtgcgcgtga ctgccgttcg gacgactcgc cagcggagcg
     1201 cggcccgatc cacgggcgcg cgaccgacgt ggagccagct cgacgacgga cggccggacg
     1261 cgctcgattc gcg
//
LOCUS       DAOQYE010000788         1272 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_140074_length_1272_cov_2.714051, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000788 DAOQYE010000000
VERSION     DAOQYE010000788.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1272
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140074_length_1272_cov_2.714051"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..975)
                     /locus_tag="PKN52_11125"
     CDS             complement(<1..975)
                     /locus_tag="PKN52_11125"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNR00549.1"
                     /translation="MARTTLSDPIPPTPSSNRRATRLVVGSGAVAVLLFLAHFFNDLF
                     SGTLAALLPTLQIRFGVSELTLALFVASLSFSSSVLQPLFGAVADRLGHRLVAAAGVI
                     VSSSILSLIAIMPSPLWLVPLLLLGGLGSAAYHPAATTLARRAASAKGGLALGVFSAG
                     GTIGLSFGPLVIGFFLIRGWLDYSPVLMVPGIVLGALIYLLTPPVHQQTGSRRAKVFD
                     VDLFRGPVGQLCLAGVLRSIAWVSMINGAPLWLVNAKGLSATDPIVFWTITVFTFSGA
                     AGGILVGLIENRFSRQFLVTGTMLLAALPLAVSSMVPVTRNWRLNRFSI"
     gene            complement(1063..>1272)
                     /locus_tag="PKN52_11130"
     CDS             complement(1063..>1272)
                     /locus_tag="PKN52_11130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00550.1"
                     /translation="VIAEQGELGSFRRLQRQPFQRQQNAGQQLTRFLGGRSGNKARYA
                     RLLAEALPLDKIPTPLGGLLASLAE"
BASE COUNT          233 a          392 c          479 g          168 t
ORIGIN      
        1 gatcgagaac cggttcagcc gccagttcct cgtgaccggc accatgctgc tcaccgcgag
       61 gggtaaggcg gcgagcagca tggtgccggt cacgaggaac tggcggctga accggttctc
      121 gatcaggccg acgaggatcc caccggcggc cccggagaag gtgaagaccg tgatcgtcca
      181 gaacacgatc gggtcggtcg cggagagccc cttggcgttc acgagccaga gcggggcgcc
      241 gttgatcatc gacacccacg cgatggagcg cagcacgccc gctaggcaga gctggccgac
      301 cggaccgcgg aacaggtcca cgtcgaagac cttggcacgc ctggaaccag tctgctggtg
      361 caccggcggc gtgagcaggt agatgagcgc accgagcacg atgccaggga ccatcaggac
      421 agggctgtag tcgagccacc cccggatcag gaagaagccg atgaccagcg gcccgaacga
      481 cagaccgatc gtgccgccgg cgctgaacac cccgagggcc aggccaccct tggcgctcgc
      541 ggcgcggcgg gcgagggtcg tcgccgcggg gtggtaggcg gccgagccga gcccgcctag
      601 gagcaggagg ggtacgagcc agaggggcga cggcatgatg gcgatgaggc tcaggatgga
      661 ggacgagacg atcacgcctg ctgccgcgac gagccggtgg ccgagccggt ccgccacggc
      721 gccgaacaag ggctgcagga ccgacgagct gaacgacagg ctggcgacga acagggcgag
      781 ggtcagctcg ctgacgccga agcggatctg cagggtgggg agcagcgcgg cgagcgtgcc
      841 ggagaacagg tcgttgaaga agtgcgcgag gaagagtagg acggcgaccg cgccgctccc
      901 gacgaccagc cgggtcgcgc gcctgttcga gcttggtgtt ggagggatcg ggtccgacag
      961 cgtcgttcgc gccatgggtg ttgagcgtac cgcggtttgc tcggttggct cttcagaacc
     1021 ggtcttgccg gcctgtgacg cgtacacgcg ttgtgtgagg ggtcactccg ccaggctcgc
     1081 cagcagcccg cccaatggcg tcgggatctt gtcgagcggg agcgcctcgg ccagtaggcg
     1141 agcgtaacga gccttgttcc cgctccgccc gccgaggaac cgagtgagtt gctgcccggc
     1201 gttctgttgg cgctggaaag gctgccgctg aaggcgccgg aaggaaccga gttcaccctg
     1261 ctcagcgatg ac
//
LOCUS       DAOQYE010000789         1272 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_140082_length_1272_cov_2.636812, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000789 DAOQYE010000000
VERSION     DAOQYE010000789.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1272
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140082_length_1272_cov_2.636812"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="PKN52_11135"
     CDS             complement(<1..299)
                     /locus_tag="PKN52_11135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00551.1"
                     /translation="MKRSLQEVVELVVFGLVALLVGTGLLWLVGWLLSLGGLLLKAVA
                     GLLWLLLRFVVPVALVAGLVYFLVKALQGRSDAAPVAGTPAAPAAPATTGAVAPEA"
     gene            355..>1272
                     /locus_tag="PKN52_11140"
     CDS             355..>1272
                     /locus_tag="PKN52_11140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00552.1"
                     /translation="MSVGVDDRASNETGGGLEVAIERTVHGGLALGRTKTGEVVLVRG
                     AIPGEKVLADVRSQKGVLMGTTLRVLEPSPDRVPVSFHPGLDFSHMAYARQLVEKRAV
                     VADALARARRQAKSIATPTPRRDAAEVAAVRPAPGGWAYRSGIQPATAPGGLGYRRPE
                     SSEVVVMGADPVATAPVDAAWQRAVALRAHAARGAVELVIRANDEGHALIAIVASTPA
                     RRLLPLAHALVHAGITGVALAPHDPRGRFRSGAERLAGERTILQRYGDVVLSVTASSF
                     AQPNPAAAGELYRELAGWAGAGESAVELFA"
BASE COUNT          200 a          450 c          471 g          151 t
ORIGIN      
        1 gcttccgggg cgaccgcgcc ggtcgttgcg ggggccgccg gggccgcggg agtaccggcc
       61 acgggcgcgg cgtcggagcg accctggagg gccttgacca ggaagtacac gaggccggcc
      121 acgagcgcca cgggcaccac gaagcgcagg agcagccata ggagaccggc gacggctttc
      181 agcagcagac cgcccagcga aagcaaccaa ccgaccaacc agagcaggcc cgtgccgacg
      241 agcagagcga ccaggccgaa gacgaccagc tccacgactt cttgtaggga gcgcttcatg
      301 aggccagtat accaaccgtc atgagcgctc ctgagtgcgg gtacactgcc cgacgtgagc
      361 gtcggcgtcg atgatcgtgc cagtaacgag acagggggcg gtctagaggt cgccatagag
      421 cgcaccgtcc acggcggcct cgcgctcggc cgaacgaaga cgggcgaggt agtcctggtg
      481 cggggggcga taccggggga gaaggtcctc gcggacgtgc gctcgcagaa gggcgtgctg
      541 atgggcacca cgctgcgggt gctcgagccg agcccggacc gtgtcccggt gagcttccac
      601 cctggcctcg atttctccca catggcctat gcccgccagc tcgtcgagaa gcgcgcggtc
      661 gtcgcggacg cgctggcgcg agcccggcgc caggccaagt ccatagccac ccccacgcct
      721 cggcgcgacg ccgccgaggt cgcggcggta cggcccgcac ccggcggatg ggcctaccgg
      781 agcggcatcc agcctgcgac cgctcccggg gggctcggct atcgccgacc ggagtcgagt
      841 gaggtcgtcg tgatgggggc ggacccggtc gccaccgcgc ccgtcgacgc ggcctggcag
      901 cgcgccgtgg cgctgcgcgc ccacgcggcc aggggcgccg tcgaactcgt gatacgggcc
      961 aacgacgagg ggcacgcgct catcgcgatc gtggcgagca ctccggcccg taggctcctc
     1021 cccttggcgc acgccctcgt gcacgcaggc atcacgggag tcgcgctggc gccccacgac
     1081 ccgcgcgggc gcttcaggtc cggggccgag cgcctcgcgg gcgaacgcac catcctgcag
     1141 cgctacggcg acgtggtcct gagcgttacc gcctccagct tcgcgcagcc caacccggcg
     1201 gcggccggcg agttgtatcg ggaactggcc ggttgggccg gggcagggga gagcgcagtc
     1261 gagctcttcg cg
//
LOCUS       DAOQYE010000790         1271 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_140296_length_1271_cov_2.284539, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000790 DAOQYE010000000
VERSION     DAOQYE010000790.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1271
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140296_length_1271_cov_2.284539"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="PKN52_11145"
     CDS             complement(<1..181)
                     /locus_tag="PKN52_11145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00553.1"
                     /translation="MSKRYGAYGNVRAETLNIAGTEVTATASELNKVDGVASTAAEID
                     YRALTATLILGTATQV"
     gene            complement(259..>1271)
                     /locus_tag="PKN52_11150"
     CDS             complement(259..>1271)
                     /locus_tag="PKN52_11150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00554.1"
                     /translation="TFDVIAREAQSALAALNAEILTMPGYSQLFSVQDDPEVEYRVGV
                     GNAFEVATEYSAPDPTRGKTTGHMLPIKPYDMALGWTMMSLRKARRAKLEADIAQMVV
                     AGRALYQKALLTRFFTSTANSVGSTSSDVPFADAGSADANYVPPESPGGETFLYTHEH
                     FLDTTDTGITSNTLDNTALSVAVEHLEEHGYQGEYMAIVSRADIGSWAAVDGFKPPTW
                     RGVNYQQSAVERAQFGDISQYFGAVETDYGMVNLWATPRVPTMNYGVVPLGLSELERP
                     LRMRIAPRIGFGINLVPGNWINAPALMVVAYAEFGVGVGNRVGGVCVDHGASTWAAPT
                     IS"
BASE COUNT          201 a          427 c          414 g          229 t
ORIGIN      
        1 agacctgcgt ggccgtgccc aggatcaggg tggcggtcag cgcccggtag tcgatctccg
       61 ctgcggtaga cgccacgcca tcgaccttgt tcagctcgct ggccgttgcc gttacctcag
      121 tgccggcgat gttgagcgtc tcagccctga cgttcccata tgctccgtat cgtttgctca
      181 tggctcctcc cgttggtggt ggggcgggct ctccggcgtg ataggatcac ctccttcccg
      241 cccgcccctg tcacctaact agctgatcgt cggagcggcc caggtcgagg cgccgtgatc
      301 gacgcacacg ccaccgacgc ggttgcccac gccaacacca aactcggcgt aggcgaccac
      361 catcagggcg ggcgcgttga tccagttgcc gggcaccagg ttgatgccaa agccgatgcg
      421 cggtgcgatg cgcatgcgca gcgggcgctc aagctcggac agacccagcg gcacgacgcc
      481 atagttcatc gtcggcacgc gcggcgttgc ccagaggttg accatcccgt agtcggtctc
      541 gacagcgccg aagtactggc tgatgtcgcc aaactgcgca cgctcgactg cggactgctg
      601 gtagttgacg ccccgccacg tcgggggctt gaagccatcc accgcagccc acgagccgat
      661 gtcggcgcgg ctgacgatgg ccatgtactc gccctggtag ccgtgctcct ccaggtgctc
      721 cacggccacg gacagcgccg tgttgtcgag cgtgttggag gtgataccgg tgtccgtggt
      781 gtccaggaag tgctcgtgcg tatacaggaa cgtctcgcca cccggcgatt cgggcggcac
      841 gtagttggcg tcagccgaac ccgcgtcagc gaagggcacg tccgaagacg tggagccgac
      901 gctgttggcg gtcgaggtga agaagcgcgt caggagcgcc ttctgataga gcgcgcgccc
      961 ggcgaccacc atctgcgcga tgtcggcctc cagcttggcg cggcgcgcct tgcgcaggct
     1021 catcatcgtc cagcccagcg ccatgtcgta gggcttgatc ggcagcatgt gcccggtcgt
     1081 cttgccccgc gtcgggtccg gtgcgctgta ctcggtggcg acctcgaagg cgttccccac
     1141 acccacgcgg tactcaacct ccgggtcatc ctgaacgctg aacagctggc tgtagccagg
     1201 catggtcagg atttccgcat tcagcgcagc cagtgcggac tgcgcctcgc gggcgatcac
     1261 gtcaaacgta g
//
LOCUS       DAOQYE010000791         1270 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_140510_length_1270_cov_2.069959, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000791 DAOQYE010000000
VERSION     DAOQYE010000791.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140510_length_1270_cov_2.069959"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(73..363)
                     /locus_tag="PKN52_11155"
     CDS             complement(73..363)
                     /locus_tag="PKN52_11155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00555.1"
                     /translation="MSMTDTIFTLSDRDDTLAHIADAMTGYEADLYAADIAAGKLEEL
                     VELEWKNVWTQGDNVYATAEAAAADATRYSPHVLEITRDDVRAALLREFATE"
     gene            complement(363..626)
                     /locus_tag="PKN52_11160"
     CDS             complement(363..626)
                     /locus_tag="PKN52_11160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00556.1"
                     /translation="MSLFYDNSPEAQAIRAANAARVARFAAMSDDELLAYDLNDDDQA
                     ERQDCFAELGRRTHIPTRNVMYGIEWPRNMAEAAKWIADGQEE"
     gene            complement(763..>1270)
                     /locus_tag="PKN52_11165"
     CDS             complement(763..>1270)
                     /locus_tag="PKN52_11165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00557.1"
                     /translation="QVRTVEDVEMIRYPIPLDILDRTHLAHRAEMPANLTAAELEPDL
                     HDRLHAAWMRVVCTQQQVLAALAHLGRLTPTEHGQAIDAGELGLNDCQEAIRSLDAGD
                     GTGPSDYWLGRCSPTAAFQRLEAWFQAALATLRVPDSRGRIERQDAGAPSGADPYERA
                     VWPMDEMP"
BASE COUNT          155 a          396 c          473 g          246 t
ORIGIN      
        1 cctcgtgccc tttcgtgggc gcgcccgccc cgtgtctcac catgggggcg ggcgcttctc
       61 tcgtgtaggg ggttactcgg tggcgaactc gcgcaggagg gcagcgcgaa cgtcgtcgcg
      121 cgtgatctcc aggacgtgcg ggctgtaccg ggtggcgtcg gccgcggcgg cttcagcggt
      181 cgcgtagacg ttgtcgccct gcgtccagac gttcttccac tcgagttcga cgagctcctc
      241 gagtttgccg gcggcgatgt cggcggcgta caggtcggct tcgtagccgg tcatggcgtc
      301 ggcgatatgg gcgagggtgt cgtcgcggtc gctgagggtg aagatcgtgt cggtcatgct
      361 catcattcct cctgcccgtc ggcgatccac ttggcggcct cggccatgtt gcggggccac
      421 tcgatgccgt acatgacgtt gcgggtgggg atgtgggtgc gccggccgag ttcggcgaag
      481 cagtcctgac gctcggcctg atcgtcgtcg ttgaggtcgt aggcgaggag ttcgtcgtcg
      541 ctcatggcgg cgaagcgggc gacgcgggcg gcgttcgcgg cgcggatggc ttgggcttcg
      601 gggctgttgt cgtagaagag gctcatggtg ttgcctttcg tggtcggggc gttgtggttg
      661 gggtctcact ccctcgccct gatgtacgta gtgtacacca gtggtttacc atctgtcaac
      721 ccccctagcc caacacccgc caaccccgtc cccaacgcga aatcagggca tctcgtccat
      781 cggccagacc gcgcgctcgt acgggtctgc gcccgatgga gcgccggcgt cctggcgctc
      841 gatccggccg cggctgtccg ggacccggag agtcgccagc gcggcctgga accacgcctc
      901 gagccgctgg aacgcggcgg tcggcgagca tcggccgagc cagtagtcgc tcggcccggt
      961 cccgtcgccg gcgtccaggc tgcggatggc ctcctggcag tcgttgagtc cgagctcgcc
     1021 tgcgtcgatc gcctggccgt gctcggtcgg cgtgagtcgg ccgaggtgag cgagggccgc
     1081 gaggacctgc tgttgcgtgc agacgacgcg catccaggcc gcgtggaggc gatcgtggag
     1141 gtctggctcc agctcggcgg ccgttaggtt ggccggcatt tcggcgcggt gcgccaggtg
     1201 cgtacggtcg aggatgtcga gagggatggg atagcggatc atctcaacat cctcgaccgt
     1261 acgcacctgg
//
LOCUS       DAOQYE010000792         1270 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_140525_length_1270_cov_1.999177, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000792 DAOQYE010000000
VERSION     DAOQYE010000792.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140525_length_1270_cov_1.999177"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="PKN52_11170"
     CDS             complement(<1..199)
                     /locus_tag="PKN52_11170"
                     /inference="COORDINATES: protein motif:HMM:NF020387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES domain-containing protein"
                     /protein_id="HNR00558.1"
                     /translation="MTSGWRIVKERFAAKAFDGEGARLYGGRWNSPGRPVVYLGCTPA
                     IAALEILAHNARLDLLEASYVI"
     gene            complement(196..654)
                     /locus_tag="PKN52_11175"
     CDS             complement(196..654)
                     /locus_tag="PKN52_11175"
                     /inference="COORDINATES: protein motif:HMM:TIGR02293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2384 domain-containing protein"
                     /protein_id="HNR00559.1"
                     /translation="MAALTWIPEVVPEAGPSGRTIERLLRSKDVSLGAASRRIEEGLP
                     FRVFAELCAGLRLTQGEFAALLQMSESTLYRRKGVGRFEATESDRLWRYVVLYARAMD
                     VLEAPDAAVEWLHAPNQALGGKSPLASTKDGPGAQRTLAVLGRMEHGVFG"
     gene            850..1179
                     /locus_tag="PKN52_11180"
     CDS             850..1179
                     /locus_tag="PKN52_11180"
                     /inference="COORDINATES: protein motif:HMM:NF025120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNR00560.1"
                     /translation="MSTNDDKFIQGSGNVFADLGLSDPRESELKARLASRIYDEIAAR
                     GWTQTKAADELGLGQPDISRLTRGMLKGFSSERLMSLLVALDYSVSIRIEGNDRPPEV
                     IEVAVPD"
BASE COUNT          218 a          429 c          423 g          200 t
ORIGIN      
        1 cgatcacgta gctcgcctcc agcaggtcga gcctggcgtt gtgcgcgagg atctcgagtg
       61 ccgcgatggc cggcgtgcaa cccaggtaga cgacgggtct gcccggcgag ttccagcggc
      121 cgccgtagag gcgcgcgccc tccccatcga acgccttggc ggcgaagcgc tccttgacga
      181 tgcgccagcc gctcgtcacc cgaagacacc gtgctccatg cggccgagca cggcgagggt
      241 ccgctgggcg ccagggccgt ccttggtgct cgccaggggg gacttgccgc ccagcgcctg
      301 gttgggggca tgcaaccact cgaccgccgc gtcgggcgcc tccagcacgt ccatggcgcg
      361 ggcgtagagg acgacgtagc gccagaggcg atcgctctcc gtcgcctcga agcgcccgac
      421 gcccttgcgg cggtagaggg tgctctcgct catctgcagc agggcggcga actcgccttg
      481 cgtcaggcgc aggcccgcgc acagctcggc gaagaccctg aacggcagac cttcctcgat
      541 gcgacgactg gccgctccca ggctgacgtc cttgctgcgc agcaggcgct cgatggtgcg
      601 gcccgatgga ccggcctccg gtacgacttc ggggatccag gtcagtgctg ccactcgtgc
      661 ctccttgctg tcatatgaca ttatcagact atcatatggg ttatgtaatc ccacgtcgga
      721 cccagcgtgg cggcaatagc gcgaagctcg gcgccgaccc agctcctctg ggcagcccgt
      781 ctctgggcag cccgccagcc tcgtactcgt ccagacacgt tatggtgaaa ctggcatagg
      841 aggcgcggcg tgagcaccaa cgatgacaag ttcatacaag gcagtggcaa cgtcttcgcc
      901 gacctagggt tgtccgaccc gcgggagagt gagctgaagg ctcgcctcgc gagcaggatc
      961 tacgacgaga tagcagcacg tgggtggacc cagacgaaag cggccgatga gctagggctc
     1021 ggtcagcccg acatctcgcg cctgacgcgc ggcatgctca agggcttctc gagcgagcgc
     1081 ctcatgagcc tcctcgtggc cttggattac agcgtgagca tccgcatcga gggcaacgat
     1141 cgcccgcccg aagtgatcga ggtggcggtc cccgactaag cccaccctcg aggcagccga
     1201 cggttcgacg cggtccggtg agcaacacga accccgctgg gttgacgagg cgcggactac
     1261 cattcgcagg
//
LOCUS       DAOQYE010000793         1268 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_140889_length_1268_cov_2.057708, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000793 DAOQYE010000000
VERSION     DAOQYE010000793.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1268
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140889_length_1268_cov_2.057708"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..895)
                     /locus_tag="PKN52_11185"
     CDS             complement(<1..895)
                     /locus_tag="PKN52_11185"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNR00561.1"
                     /translation="MSRAAPAVTFGLALASLGQRPVRTSLTAVGIAVAVAGAIVFLSL
                     GEGIRRVFADQLANLGPDIQVTYGPASGDFFPTTPDLPGHYLQDLTAAAPTLGIRSVV
                     PVLVYLRGGFGPSSSFVIEGLPAAVDLGELFHGATAHAGRGLVPGDEGAAVAVVGSSA
                     AERGGLDVGSPLRLNPTTSLEVVGIVGAGGGLLDNLIVVPLTTLQAALGVTDRYSLLA
                     VSSERPERAEAVAAAVRERFPALGARTSSELFAQVNASLRVSDVVRLGISAIALIVGA
                     IAVANTVMMSVFERTREFGVIR"
     gene            complement(918..>1268)
                     /locus_tag="PKN52_11190"
     CDS             complement(918..>1268)
                     /locus_tag="PKN52_11190"
                     /inference="COORDINATES: protein motif:HMM:NF024855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HNR00562.1"
                     /translation="VEQRFDLTESRTVELDLTAVAAGPEAESTASVPIGATEIVIAIK
                     ATSWLEVYRGSARNEGERLVYTTAAPGQTYRFTLPVYVHAGNAAGVEVTLGDGVPFAM
                     GSSGAVVGRAFQAQ"
BASE COUNT          192 a          456 c          470 g          150 t
ORIGIN      
        1 ccctgatcac cccgaactcc cgcgtgcgct cgaagacgct catcatgacc gtgttggcca
       61 ccgcgatcgc gccgacgatc agggcgatgg cgctgatgcc gaggcgcacg acgtccgaca
      121 cgcgcaggct ggcgttgact tgagcgaaga gttccgagct cgtccgcgcg cccagggccg
      181 ggaagcgctc ccgaaccgcc gccgccaccg cctcggcgcg ctccggccgc tcgctcgaga
      241 cggccaggag gctatagcgg tcagtcactc ccagggccgc ctgcagcgtc gtgagcggca
      301 cgacgatgag gttgtcgagg agcccgccgc cggcgccgac gatccccacc acctcgaggc
      361 tggtggtcgg gttcagccgc aacggcgagc cgacgtcgag gccgccacgc tcggccgcgc
      421 tcgagccgac gacggccacg gccgccccct cgtcgcccgg caccaggccg cgcccggcgt
      481 gcgccgtcgc gccgtggaag agctcgccaa ggtcgacggc ggccggtaac ccctcgatca
      541 cgaacgacga cgaggggccg aagccgccgc ggaggtagac gaggacgggc acgaccgaac
      601 ggatccctag cgtcggcgcc gcggccgtca ggtcctgcag gtagtggccc gggaggtccg
      661 gcgtggtggg gaagaagtcg ccggaggcgg ggccgtacgt gacctggatg tcgggtccga
      721 ggttggcgag ctggtccgcg aagacgcgcc tgatcccctc gcccaacgac aggaagacga
      781 tggcccccgc cacggcgacg gcgatgccga ccgccgttag cgacgtgcgc accggccgct
      841 gaccgaggga ggcgagcgcc aaaccgaagg tgacggcggg tgccgcccgg ctcatgcgcg
      901 ggcggggagg agcggggtca ctgcgcctgg aaggcccgtc ccaccaccgc accggaggat
      961 cccatcgcga acggcacgcc gtcgccgagg gtcacctcca cgcccgccgc gttgcccgcg
     1021 tggacgtaga cgggcagcgt gaagcggtag gtctgaccgg gggcggcggt ggtgtacacg
     1081 agccgctccc cctcgttgcg ggccgaccca cggtagacct cgagccagct cgtggccttg
     1141 atggcgatga cgatctcggt ggcgccgatc gggacgctcg cggtggactc cgcctcgggc
     1201 cccgccgcga cggccgtcag gtcgagttcg acggtgcggc tctcggtgag atcgaagcgc
     1261 tgctcgac
//
LOCUS       DAOQYE010000794         1267 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_141043_length_1267_cov_2.377888, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000794 DAOQYE010000000
VERSION     DAOQYE010000794.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1267
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141043_length_1267_cov_2.377888"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1093)
                     /locus_tag="PKN52_11195"
     CDS             complement(<1..1093)
                     /locus_tag="PKN52_11195"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HNR00563.1"
                     /translation="MAVAFAAAIAGTRSSGSTSAAVDLSARSAGELAFVLISRSNTDA
                     PGSTPSGWTLLEEQNSTYRVQLYYKVLASGDIGTVTWSGYANSVRTAATAFIYTGQAD
                     SSPVAADTHGTGTWSTTGARTIDFGSLASTSRLMVGFASWYGTTSRNVNSQPSGMTER
                     YDDHNTTPDWGHYAADQLWTSGTYAPSSSTNYLSGNATYRVGFLVALAHKSTAPAITG
                     LSPAKGLPAGGTSVVITGTDFTGATAVKFGGTNAASYTVDNDLQITATTPAKSAGTYQ
                     TQVTTPDGSSSDVAADNFEFTSALPPWLVAGQVAYAYSSGSTASVALPGNVTNGNTVI
                     VFGDGGDGCTFTGVTKTAGTATIGTPVEVV"
BASE COUNT          186 a          371 c          490 g          220 t
ORIGIN      
        1 ggacgacctc tactggcgtc ccgatggtgg ccgtaccagc ggtcttagtg acgccggtga
       61 aggtgcagcc atcgccgccg tcaccgaaca cgatgacggt gttgccgttg gtgacgttgc
      121 ccggcagcgc gaccgaggcg gtggagccgc tagagtaggc gtaggcgacc tgaccggcga
      181 ccagccacgg cggcagcgcg gaggtgaact cgaagttatc ggcggctacg tcggatgagg
      241 agccgtccgg ggtggtgacc tgcgtctggt aggtgccggc cgacttggcg ggcgtggtgg
      301 cggtgatctg caggtcgttg tcgaccgtgt agctggccgc gttggtgccg ccgaacttga
      361 ccgccgttgc gcccgtgaag tcggtgccgg tgatgacgac gctggtgccg cctgcgggca
      421 ggcccttggc aggggagagg ccggtgattg cgggcgcggt cgacttgtgc gcgagtgcga
      481 ccaggaagcc gacgcggtac gtcgcgttgc cggagaggta gttggtcgag ctactcggcg
      541 cataggtgcc ggacgtccag agctggtctg cggcgtagtg gccccagtcg ggcgtcgtgt
      601 tgtggtcgtc gtagcgctcg gtcatgcccg agggctgcga gttgacgtta cgcgaggtgg
      661 ttccgtacca cgacgcgaag ccgaccatca gacggctggt ggaggccagc gagccgaagt
      721 cgatggtccg cgccccggtc gtcgaccagg tgccggtgcc gtgcgtatca gcggctaccg
      781 ggctggagtc ggcctgcccg gtgtagatga acgcggtcgc cgccgtgcgg acgctattgg
      841 cgtagcccga ccaagtaacg gtgccgatgt caccggaggc gagcaccttg taatagagct
      901 ggacgcggta ggtgctgttc tgctcctcca gcagggtcca gccgctgggg gtgctgccgg
      961 gtgcgtcggt gttcgagcgc gagatgagca cgaacgccag ctccccggcc gaccgcgccg
     1021 agaggtctac cgcggcactg gtcgagccgg aggagcgggt gcccgcgatt gcggctgcga
     1081 aggcgacagc cacggcgcta cccggtcagg ccagggatca tgcggccgag tcgccctcga
     1141 ccttgagcac gaagctgttc gcgttgtagg ccgcgcaact ggcgggcacg atgcgcttga
     1201 cccagatgat gtagtactgg ttctgggtca gcgtgcccag cgacagtccg gttgccttgc
     1261 tggtgca
//
LOCUS       DAOQYE010000795         1267 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_141072_length_1267_cov_2.139439, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000795 DAOQYE010000000
VERSION     DAOQYE010000795.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1267
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141072_length_1267_cov_2.139439"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /locus_tag="PKN52_11200"
     CDS             <1..624
                     /locus_tag="PKN52_11200"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNR00564.1"
                     /translation="VGAGRDVLRTAGVPLAYFLRFSAWLGRTMLVPFLPLFIATITVD
                     KDLAGMYTGLAIGLAAGSSTLSGVFLGRLSDRIGYKPVLVASTFACALFYVPMAYVGN
                     VFQLIAVNVLIGFAIGGVLPAISAMLARLTPPQVAGAVYGIDNSVSAASRALAPIVSG
                     AVVTMTSLPGETNYRTIFLVTAALFFLTAVVAAWFLPKRGAEPEPIA"
     regulatory      678..783
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            823..>1267
                     /locus_tag="PKN52_11205"
     CDS             823..>1267
                     /locus_tag="PKN52_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNR00565.1"
                     /translation="MRKFPTILVCLALALLGLGAAQRLTVLTHDSFALPSELLAEFTA
                     RTGIQVVFLQGGDAGEVVNRAVLTKARPLADLLFGVDDSLLERVRSEGIFEPYASGEL
                     ARVDGALLLDVGGLVTPVDVGYVLPNIDTAWFAERGLVLPAALEDL"
BASE COUNT          172 a          454 c          421 g          220 t
ORIGIN      
        1 gtcggcgcgg ggcgcgacgt gctgcgcacc gccggcgtgc ccctcgccta cttcctgcgt
       61 ttcagcgcct ggctgggccg caccatgctg gtgccgttcc tgccactgtt catcgccacc
      121 atcacggtcg acaaggacct ggcgggtatg tacacgggtc tcgccatcgg cctcgccgcc
      181 ggctcctcga cgctgagcgg cgtcttcctc ggccgcctga gcgaccgcat cggctacaag
      241 cccgtgctgg tggcgtccac cttcgcctgc gcgctcttct acgtgcccat ggcgtacgtc
      301 gggaacgtct tccagctcat cgccgtcaac gtgctgatcg gcttcgccat cggcggggtg
      361 ctgccggcca tcagcgcgat gctcgcacgc ctcacgccac ctcaggtggc cggggccgtc
      421 tacggcatcg acaactccgt ctcggcggcc tcgcgggcgc tggcgccgat cgtctccggc
      481 gccgtcgtca ccatgacgag cttgccaggc gagacgaact accgcaccat cttcctcgtc
      541 accgccgcgc tgttcttcct gaccgccgtg gtggccgcct ggttcctgcc gaagcgcggg
      601 gccgagccgg agccaattgc ctagcgacag gggtatcccg aaccgttgac cacccgctca
      661 gtaggcggtt agcctcgtag cgtcaccagg ggtgtccggc gcccatccaa gcgccgggct
      721 gagatcacac cctagaacct gaccccggtc atgcgggcgg agggagagtg acgcagcgcg
      781 ccaagcgccg tgcgtccgta ggtgacggaa ggacgtgcga ccatgcgcaa gtttccgacc
      841 atcctcgtct gtctggcact agcgctgctc ggcttgggtg ccgcgcagcg cctcaccgtg
      901 ctcacgcacg attcgttcgc gctgccgagc gagctgttgg cggagttcac ggcgcgtacc
      961 ggcatccagg tcgtgttcct gcagggaggc gacgccggcg aggtagtgaa ccgtgccgtg
     1021 ctgaccaagg cccggccgct ggcggacctg ctcttcggag tggacgacag cctgctcgaa
     1081 cgggtcagga gcgagggcat cttcgagcca tacgccagcg gcgagctcgc ccgcgtcgac
     1141 ggagcgctgc tgctcgacgt cggcggtctc gtgacgccgg tcgacgtcgg ctacgtgcta
     1201 cccaacatcg ataccgcatg gttcgcggag cgcgggctcg tgctcccggc ggcgctcgag
     1261 gacctgg
//
LOCUS       DAOQYE010000796         1265 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_141437_length_1265_cov_2.620661, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000796 DAOQYE010000000
VERSION     DAOQYE010000796.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141437_length_1265_cov_2.620661"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1265)
                     /locus_tag="PKN52_11210"
     CDS             complement(<1..>1265)
                     /locus_tag="PKN52_11210"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNR00566.1"
                     /translation="FRRQEVQDLVALAGALADPANTLALGALLRGPAVGVTDEELLDT
                     AYALSEGASGGGERPTGARASAGARLTLETAPDLIPVPRIAEVVRRLSLLSARRFTST
                     PYAVLSEAVEALELRAVLVNRHGRQAARALANLERFLQGAKAYALRGLRAFADDMWAA
                     WSDGSTEREGYVDAVEDAITLITMHSAKGLEWRVVVPVGSMSAPGGARGPWYSRQAGR
                     PVFDVLGHKGSSAERLAELEKAEQHAERLRLWYVTATRARDLLVVPEFGFATGAAAWC
                     NLVAWQKEVEWAYVEAPEPAEPAAGEPAAWYAVEAQTPEEFAAQTAAVTANARLIERR
                     APSRQDDARPGGAGVIPAQPVFTGELGARILATLDEVEEAEPDSALNVGSVRGIVLHK
                     LLEEIINGEFRDGSSAGTRPQTAADIVEA"
BASE COUNT          168 a          477 c          436 g          184 t
ORIGIN      
        1 tcgcctcgac gatgtctgcc gccgtctgcg gccgtgtgcc cgcagacgag ccgtcgcgga
       61 actccccgtt gatgatttcc tcgaggagct tgtggagcac gatgccccta acgctgccaa
      121 cattcaaggc gctatcgggc tcggcttcct cgacttcgtc cagcgtggcg aggatcctcg
      181 ctccgagctc acccgtgaac acgggctgcg ccggtatgac ccccgctccg cctggtcgcg
      241 catcgtcctg acggctgggc gcccgccgct cgatgagcct ggcgttggcg gtcaccgccg
      301 cggtctgggc ggcgaactcc tcgggcgtct gcgcctcgac ggcgtaccag gccgccggct
      361 cgccggccgc cggctcagcc ggctcgggcg cttcgacgta ggcccactcg acctccttct
      421 gccaagcgac gaggttgcac caggccgcgg cgcccgtggc gaagccgaac tccggcacca
      481 ccagcaagtc gcgcgcccgc gtcgcggtca cgtaccagag gcgcagccgc tcagcgtgct
      541 gctcggcctt ctcgagttcc gccaagcgct cggcggaaga gcctttgtga ccgagcacgt
      601 cgaagaccgg tcggcccgcc tggcggctgt accacggccc gcgcgcgccg cctggtgctg
      661 acatcgagcc gacgggcacc accacccgcc actcgagacc cttcgcggag tgcatggtga
      721 tcagcgtgat cgcgtcctcc acggcgtcga cgtaaccttc gcgttcggtg gagccgtcgc
      781 tccaggccgc ccacatgtcg tcggcgaagg cccgcagccc gcgcagcgcg tacgccttgg
      841 cgccctgcag gaaccgttca aggttggcga gcgcccgcgc cgcctgcctc ccgtgccggt
      901 tgacgagcac ggcgcgcaac tccagcgcct cgacggcctc ggagaggacg gcgtacggag
      961 tggaggtgaa gcgccgcgcg ctcaagagcg agagccgcct gacgacctcc gctatgcgcg
     1021 ggaccgggat gaggtcgggc gctgtctcga gggtcaggcg tgcgcccgcg gacgcacggg
     1081 cgccggtcgg gcgctcgccg cctcctgagg cgccttcgga gagggcgtac gcggtgtcga
     1141 ggagctcctc gtcggtcacg ccgaccgccg gccccctgag caacgccccc aacgctagcg
     1201 tgttggcggg gtcggccagg gcgcccgcga gcgccacgag gtcctgcacc tcctggcgcc
     1261 ggaac
//
LOCUS       DAOQYE010000797         1264 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_141585_length_1264_cov_3.171216, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000797 DAOQYE010000000
VERSION     DAOQYE010000797.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1264
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141585_length_1264_cov_3.171216"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="PKN52_11215"
     CDS             complement(<1..358)
                     /locus_tag="PKN52_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNR00567.1"
                     /translation="MNHRNVAIIAHVDHGKTTLVDGLLRQSNVFRANQQVAERVMDSG
                     DIERERGITILAKNTAVEWKGEKINIVDTPGHADFGGEVERALTMVDGVLLLVDAAEG
                     PMPQTRFVLRKALARGL"
     gene            complement(428..757)
                     /locus_tag="PKN52_11220"
     CDS             complement(428..757)
                     /locus_tag="PKN52_11220"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HNR00568.1"
                     /translation="MLADISEAIERITSYLQGLSVTEFLTDRRTQDAVIRNLEVIGEA
                     VKLLSDASQAAYPDVPWRLMARSRDRLIHGYFGVNLDIVWHIAATELPRVATALSRPD
                     APQKTPG"
     gene            complement(774..1073)
                     /locus_tag="PKN52_11225"
     CDS             complement(774..1073)
                     /locus_tag="PKN52_11225"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNR00569.1"
                     /translation="MPSDDVMSLLRYEQPVLRQRFGVARLGVFGSVASGRTSSESDVD
                     IVVEFDRPIGLEFVNLAEHLERILGRNVDLITPEGVASIRAPGVANSIMRSVVYV"
BASE COUNT          231 a          381 c          407 g          245 t
ORIGIN      
        1 tgaggccgcg cgccagtgcc ttgcggagca cgaagcgtgt ctgtggcatg gggccctccg
       61 cggcgtcgac gagcagcagg acgccgtcga ccatcgtcag ggcgcgctcc acctcgccgc
      121 cgaagtcggc gtggcccggg gtgtcgacga tgttgatctt ctcgcccttc cactcgacag
      181 ccgtgttctt ggcgaggatg gtgatgccgc gctcgcgttc gatgtcgccg gaatccatga
      241 cgcgttccgc gacctgctgg ttggcgcgga agacgttcga ttggcgcaga agcccgtcga
      301 ccagcgtggt cttgccgtgg tcgacgtggg cgatgatggc gacgttccta tggttcattg
      361 gctctccgtg tccgactaag cccggcggtt gacgcgggcg gaacgggaga gtatagcgga
      421 aggaaggcta gcccggagtt ttttggggag cgtcgggtcg actcagggcc gttgcgactc
      481 gaggcaactc ggtagcggca atatgccaca cgatgtccag gttgaccccg aagtagccgt
      541 ggatgagcct gtcccgggag cgggccatga gccgccacgg gacatccgga tacgccgctt
      601 gcgaggcatc cgagaggagc ttgaccgcct ccccgatcac ttcgaggttc ctgatgaccg
      661 catcctgcgt ccgacggtcc gttaagaact cggtgacgga cagcccttgg aggtatgagg
      721 tgatgcgttc gatcgcctcg cttatatccg ctaacaacag cgagtccggt cgcctagaca
      781 taaacgaccg aacgcatgat gctattggcg actcccggcg ctcgaatcga ggcgacacct
      841 tcgggtgtga tgaggtcaac gttccggccg aggatgcgtt ccagatgttc agcgaggttc
      901 acgaactcca gcccgatagg tcgatcgaac tcaactacta tgtcgacatc gctttcactc
      961 gacgttctgc cagaagccac cgacccgaaa acgcccagcc tagctacgcc gaagcgctga
     1021 cgcaggactg gctgctcgta cctcagtaaa gacatcacgt cgtcactagg catcgcttgg
     1081 tccatagtgt ccgctcggtc caccgtgttc gtattttacg cctagtctcc agatcggtcc
     1141 ttggctgaca gggcagtcag gaggggtggg caaggggacc gttcgggcac ctacgggtaa
     1201 gctgtgccaa tgaccgaccg ccaagccgac atcgggctcg taggcctcgc cgtcatgggc
     1261 caga
//
LOCUS       DAOQYE010000798         1263 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_141748_length_1263_cov_4.439570, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000798 DAOQYE010000000
VERSION     DAOQYE010000798.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1263
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141748_length_1263_cov_4.439570"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1003
                     /locus_tag="PKN52_11230"
     CDS             <1..1003
                     /locus_tag="PKN52_11230"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNR00570.1"
                     /translation="FGAHPVGTGPYKLVEWVLGQRLVLEANDDYWNGRPSVDRLIFRP
                     IPEASTRLAELQAGTVDIITGLNYDAIPLVDADPSLRVEANSGRRTVMMHMDLLNGAK
                     PLQDVRVRQAMSYAVDRQLLIDAVLNGYGTPLATIFRPDMAGYSADFQPYPYDPERAK
                     ELLAEAGYPNGFTIRFMTSDGIVNKGVEVAEALAAMLGDVGIKVEVLPVALQVMRDMY
                     IGNPDPAGGNVEPLFMFNYGAAIPDATSPLKALIQTGGIEAFYEDPVMNELINEYGSE
                     TNVEKRNQVAHEIQEKLYNDLPVIALYLQLDTYGVNERIDWKARKDEYILGKDIALR"
BASE COUNT          236 a          458 c          371 g          198 t
ORIGIN      
        1 gttcggtgcg cacccggttg gcacgggccc ctacaagctc gtcgagtggg tgctcggcca
       61 gcgcctcgtg ctcgaggcca acgacgacta ctggaacggc cgacccagcg tagaccgcct
      121 gatcttccgc ccgatccccg aggcctccac gcgcctcgcc gaactccagg ccggcaccgt
      181 cgacatcatc accggtctca actacgacgc catcccgctc gtcgacgccg accccagcct
      241 gcgcgtcgag gccaactccg ggcgccgtac cgtgatgatg cacatggacc tgctcaacgg
      301 cgccaagccc cttcaagacg ttcgcgtccg gcaggccatg agctacgccg tcgacaggca
      361 actcctcatc gacgccgtcc tgaacggcta cggcacgccg ctcgcgacga tcttccgccc
      421 cgacatggcg ggctactccg ccgacttcca gccctacccg tacgacccag agcgtgccaa
      481 ggagctgctg gcggaggccg gctacccgaa cggcttcacc atccgcttca tgacgagcga
      541 cggcatcgtc aacaagggcg tcgaggtggc cgaggcgctt gccgccatgc tcggtgacgt
      601 cggcatcaag gtcgaggtcc tgcccgtggc gctgcaggtc atgcgcgaca tgtacatcgg
      661 caacccggac ccggccggcg gcaacgtcga acccctgttc atgttcaact acggtgccgc
      721 catcccggac gccaccagcc ccctgaaggc cctcatccag accggcggca tcgaagcctt
      781 ctatgaagac cccgtcatga acgagctaat caacgagtac ggctcggaga cgaacgtgga
      841 gaagcgcaac caggtcgccc acgagatcca ggagaagctc tacaacgacc tacccgtcat
      901 agcgctctac ctccagctcg atacctacgg cgtgaacgag cggatcgact ggaaggctcg
      961 caaggacgag tacatcctcg gcaaggacat cgccctccgg taacccgccg cttcgccggt
     1021 ggtggccgcc ctcctcggag agagcggcca ccacccgagc cttgacgaac ttccaaacga
     1081 tcgaggagag atgggccgtt acgtagtcag gcgttcattg caggccgtgt tcgtgatctt
     1141 ggccgtgctc gtggtcacgt tcatactcct gcgtgcgatc ggcgatccgg ccaggctcct
     1201 cgtgagcccg gagggttcgc gcgagaacct ggcgcagatc cgtcacgccc tcgggctcga
     1261 cgt
//
LOCUS       DAOQYE010000799         1263 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_141759_length_1263_cov_3.741722, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000799 DAOQYE010000000
VERSION     DAOQYE010000799.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1263
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141759_length_1263_cov_3.741722"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(78..153)
                     /locus_tag="PKN52_11235"
     tRNA            complement(78..153)
                     /locus_tag="PKN52_11235"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(118..120),aa:Ala,seq:cgc)
     gene            331..>1263
                     /locus_tag="PKN52_11240"
     CDS             331..>1263
                     /locus_tag="PKN52_11240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00571.1"
                     /translation="MVPERVKRRNFLLGVINGILVNGGEAFLHSGLVMAPFLASIGAP
                     AVVIGLVPALRVGTWFLPQLLVANRISPEPLKLRFYHFTSTVRTVALLTMTASVFLVG
                     GGRPALMTTILLVMITINAMAGGIGGVPFADVTAKIVPHARLGTFWALRNSIGGILAL
                     GAGFVLRAVLESDIGFPDNFGLIFLLGTVLSGFAYASFSLVKEPPGVPGLKRPLVGML
                     REIPTLLRVDVGLRRFLRVRFLGLAALLAEPFYAVYAITSLGAPESAIGFYVMAATAA
                     TIVGNYAFRLPADRGRNVFVMQVGYFSLLAAPLAA"
BASE COUNT          167 a          456 c          399 g          241 t
ORIGIN      
        1 ggaagtgccc gtacgtcccg ctctcgcaag atgacggacg gcaccctggt gagatgccgt
       61 cctacacggt gtttctttgg tggagctgag gggattcgaa cccctgacct cgtgagtgcg
      121 attcacgcgc gctcccaact gcgccacagc cccgagaagc cggcggtccg caggcggacc
      181 gggcaacggg aagtgtaagt gttgcgcctc tgaagtgtca aacgaccagc gaacgcgggg
      241 tcgctcgacg gctcgcctca ggccgcccac cggtcgcgac ccgccggtcg cctgagcaac
      301 gccggccgga gcgctatagg atgtcgggag atggtgcctg agcgcgtcaa gcggcgcaac
      361 ttcctcttgg gcgtgatcaa cgggatcctc gtcaacggtg gcgaggcttt cttgcattcc
      421 ggcctcgtga tggcgccgtt cctcgcctcg ataggcgcgc ctgccgtcgt catcggactc
      481 gtcccggcgc tgcgcgtcgg aacctggttc ctgccacagc tcctcgtcgc gaaccgcatc
      541 tcgcccgagc cgctcaagtt gcgcttctac cacttcactt cgaccgtgcg cacggtcgcg
      601 ctcctgacca tgacggcctc cgtgttcctg gtcggcggcg gtcgccctgc cctgatgacg
      661 acgatcctcc tggtcatgat cacgatcaac gccatggccg gcgggatagg gggagtcccc
      721 ttcgcggacg tcaccgccaa gatcgtgccg cacgcgcgcc ttggcacgtt ctgggccctg
      781 cgcaactcca tcggcggcat cctcgcgctg ggcgccggct tcgtgctccg cgcggtgctc
      841 gagtccgaca tcggcttccc tgacaacttc ggcttgatct tcctactagg gaccgtcctg
      901 tcggggttcg cctacgccag cttctcgctg gtcaaggagc cgccgggcgt tcctgggctg
      961 aaacggcctc tggtgggcat gctgcgcgag atcccgaccc tcttgcgcgt cgacgtcggg
     1021 ctgcggcgct tcctgcgcgt gcgcttcctc gggctggccg cgctcctcgc cgaaccgttc
     1081 tacgccgtgt acgccatcac gtcgctcggc gccccggaga gcgccatcgg cttctacgtc
     1141 atggccgcca cggccgccac catcgtcggg aactacgcgt tcaggctgcc ggccgaccgc
     1201 gggcgtaacg tcttcgtcat gcaggtcggg tacttctcgc tcctcgccgc tcccctcgcc
     1261 gcg
//
LOCUS       DAOQYE010000800         1259 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_142585_length_1259_cov_2.294020, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000800 DAOQYE010000000
VERSION     DAOQYE010000800.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1259
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142585_length_1259_cov_2.294020"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="PKN52_11245"
     CDS             complement(<1..278)
                     /locus_tag="PKN52_11245"
                     /inference="COORDINATES: protein motif:HMM:NF013935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HNR00572.1"
                     /translation="MAKIKLTKNELKKQKDALKMYKRYLPTLQLKKQQLQTEIRGIEA
                     RARDIAAERDRHLAEFQAWVAVFGEEDAIKRPDGGWILSSGTIRTSVG"
     gene            complement(280..>1259)
                     /locus_tag="PKN52_11250"
     CDS             complement(280..>1259)
                     /locus_tag="PKN52_11250"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF002555.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HNR00573.1"
                     /translation="GGPSVNPVNRIIPREMIRTGIPMIDLFNTLVKSQKLPIFSVSGE
                     PYNQLLARIAMQAEVDLIILGGMGLKYDDYLFFKDTLEEGGALQNTVMFVHTAADPVV
                     ECLMVPDISLAVAERFALKGLNVLVLLTDMTNFSDSLKEIAITQEQVPSNRGYPGDLY
                     SQLAARYEKAVDFAESGSITLLAVTTMPGDDVTHPVPDNTGYITEGQFYLKGGRIEPF
                     GSLSRLKQMVNGKTRADHRSIMDGMIKLFAAYRDTLEKKSMGFQMSAWDQKLLKYGEL
                     FEREMMDLSVNIPLEKALDKGWEILADCFEPQETGLQTKLIEQFWPKKA"
BASE COUNT          195 a          370 c          427 g          267 t
ORIGIN      
        1 ttcccgacgc tcgtgcgtat cgtccccgac gagaggatcc agccgccgtc ggggcgcttg
       61 atcgcgtctt cctcgccgaa gaccgcgacc caggcctgga actcggccag gtgccggtcg
      121 cgctcggcgg cgatgtcccg ggccctggcc tcgatcccgc ggatctcggt ctgcagctgc
      181 tgtttcttga gctggagcgt gggaaggtag cgcttataca tcttgagcgc gtccttctgc
      241 ttcttcagct cattcttggt cagcttgatc ttggccatct tacgccttct tgggccagaa
      301 ctgctcgatc agtttggtct gtaggcccgt ctcctgcggc tcgaagcagt cggccagtat
      361 ctcccagccc ttgtccaggg ccttctcaag ggggatgttc accgagaggt ccatcatctc
      421 gcgctcgaag agctcgccgt acttcaagag cttctggtcc caggcgctca tctggaagcc
      481 catggacttc ttctccaacg tgtcgcggta ggcggcgaag agcttgatca tgccgtccat
      541 gatggagcgg tggtcggcgc gggtcttgcc gttgaccatc tgcttcaggc gcgacaggga
      601 gccgaagggc tcgatacggc cgcccttcag gtagaactgg ccctcggtga tgtagccggt
      661 gttgtcgggc accgggtggg tcacgtcgtc gccgggcatc gtggtcaccg ccaggagcgt
      721 gatggagccg gactcggcga agtcgaccgc cttctcgtag cgggcggcca gctggctgta
      781 gaggtcgccg ggatagccgc ggttggacgg tacctgctcc tgggtgatgg cgatctcctt
      841 tagggagtcg gagaagttgg tcatgtcggt gaggagcacg agtacgttga ggcccttcag
      901 ggcgaagcgc tcggccacgg ccagggagat gtcggggacc atgaggcatt ccaccaccgg
      961 gtcggcggcc gtgtgcacga acatcacggt gttctgtagg gcgccgccct cctccaaggt
     1021 gtccttgaag aaaaggtagt cgtcgtactt aaggcccatg ccgcctagga tgatgaggtc
     1081 tacctcggcc tgcatggcga tgcgcgccag gagctggttg tacggctcgc cggagaccga
     1141 gaagatcggc agcttctggc tcttcaccag ggtgttgaag aggtcgatca tcgggatgcc
     1201 ggtgcgtatc atctcccggg ggatgatgcg gttgaccggg ttgaccgagg ggccgccga
//
LOCUS       DAOQYE010000801         1257 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_142889_length_1257_cov_3.168053, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000801 DAOQYE010000000
VERSION     DAOQYE010000801.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1257
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142889_length_1257_cov_3.168053"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1257
                     /locus_tag="PKN52_11255"
     CDS             <1..>1257
                     /locus_tag="PKN52_11255"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HNR00574.1"
                     /translation="PGVDTVVLDEARARELAAAAGSAAWRVAGLEEREQTRSPAAPFT
                     TSTLQQEAGRKLGLSARDTMRVAQSLYENGYITYMRTDSTALSQEALNAARGAITRRY
                     GADYLSQGERKHKGAARNAQEAHEAIRPAGTEMKTAQEHGLKGVEAALYDLVWKRTVA
                     SQMADARLKFVTARITARSGSDELTFRATGRSVLFPGFFRAYVEGSDDPEAALDDRDQ
                     PLPLLATGDGLGCQSVTAEGHETKPPARFTEASLVKLLEAEGIGRPSTYASIIDTIQA
                     RGYARKQGQQLVPTFTAFATNNLLERQFRQLVDTEFTAQMEQVLDDIAAGERASGAYL
                     RDFYLGNEGIVRLVDDALEAIDARQISTVENPAWAPYVVRVGRYGPYVEGPLDGETRT
                     ASLPPDVAPGDLTKDDLTRYLTEGNMG"
BASE COUNT          214 a          446 c          443 g          154 t
ORIGIN      
        1 ccgggcgtcg acacggtcgt cctcgacgag gccagggctc gcgagctcgc ggcagcggcc
       61 gggtcggccg cctggcgggt cgccggactg gaagagcgcg agcagacgcg ctcgccggcg
      121 gcgcccttca cgacctcgac cctgcagcag gaagccggcc gcaagctcgg cctctccgcg
      181 cgcgacacca tgcgcgtggc gcagagcctg tacgagaacg gctacataac gtacatgcgc
      241 accgactcga ccgccctctc gcaggaggcc ctgaacgcgg cgcggggcgc catcacgcgg
      301 cgttacggcg ccgattacct cagccagggc gagcgcaagc acaagggcgc cgcgcgcaac
      361 gcgcaggagg cccacgaggc catccggccg gccggcacgg agatgaagac cgcccaggag
      421 cacggactga agggcgtcga ggcggcgctc tacgacctcg tctggaagcg caccgtggca
      481 tcgcagatgg cggacgcgcg cctcaagttc gtgacggcac ggatcacggc caggagtggc
      541 tcggacgagc tgaccttccg ggctacgggc cgcagcgtgc tcttcccggg cttcttcagg
      601 gcctacgtgg agggctccga cgatcccgag gccgcgctcg acgaccgcga ccagccactg
      661 cccctcctcg ccacgggtga cgggctgggt tgccagagcg tcacggccga aggtcacgag
      721 acgaagccgc ccgcgcgctt cacggaggcg tcgctcgtca agctcctcga ggccgagggc
      781 atcggccgtc cgagcactta cgcgagcatc atcgacacga tccaggcgcg cggctacgct
      841 cgcaagcagg gccagcagct cgtgccgacc ttcacggcgt tcgccaccaa caacctcctt
      901 gagcgccagt tcaggcagct cgtcgacacc gagttcacgg cgcagatgga gcaggtcctg
      961 gacgacatcg cggcgggcga gcgggcgtcc ggggcttacc tgcgcgactt ctacctcggg
     1021 aacgaaggca tcgtgcgtct cgtagacgat gcgctcgagg cgatcgacgc gcgccagatc
     1081 tcgacggtcg agaacccggc gtgggcgccg tacgtcgtgc gcgtcgggcg ttatgggccg
     1141 tacgtcgagg gcccgctcga cggcgagacc cgcacggcgt cgctgccacc cgacgtggcg
     1201 ccgggcgacc tgacgaagga cgacctgacc aggtacctga ccgaaggcaa catgggc
//
LOCUS       DAOQYE010000802         1255 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_143351_length_1255_cov_1.874167, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000802 DAOQYE010000000
VERSION     DAOQYE010000802.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1255
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143351_length_1255_cov_1.874167"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..617
                     /gene="rplA"
                     /locus_tag="PKN52_11260"
     CDS             <1..617
                     /gene="rplA"
                     /locus_tag="PKN52_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HNR00575.1"
                     /translation="VKELASAKFDETVEAHFRLGIDPRKSDQNVRGTVALPHGTGKTV
                     RVLAITQGDAVAAAEGAGADFVGGTDIIERILGGWMDFDAVVATPDMMAAVGSKLGRV
                     LGPRGLLPNPKAGTVGPDIGGIVRALKAGQIEFRADKTGVVHAPIGKASFAPAQIAEN
                     FAALRSAVEAAKPEAARGQYVKTIFLTSTMGPSVRIALGAVTEA"
     gene            817..>1255
                     /gene="rplJ"
                     /locus_tag="PKN52_11265"
     CDS             817..>1255
                     /gene="rplJ"
                     /locus_tag="PKN52_11265"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HNR00576.1"
                     /translation="MANPRNVTAVAELREQLGDAETFFLVDYQGLSAGDLGRLRAAVR
                     SAGGRMLVAKNTLINVVLKEQGIEGFEATLQGPTALVLVGDELVAPVKAITEFAKAHP
                     KELPKSKGGRLEGSALGEDALVRIAKLPSKQQIQSQLVGVLIAP"
BASE COUNT          212 a          436 c          426 g          181 t
ORIGIN      
        1 tcgtcaagga gctcgcgagc gccaagttcg acgagaccgt cgaggcgcac ttccgtctcg
       61 gcatcgaccc gcgcaagtcc gatcagaacg tccgtggcac cgtcgccctg cctcacggca
      121 ccggcaagac ggtccgcgtc ctcgccatca cccagggcga cgccgtcgcg gcagccgagg
      181 gcgccggggc cgacttcgtg ggcggcaccg acatcatcga gcgcatcctc ggcggttgga
      241 tggacttcga cgccgtggtg gccacgcctg acatgatggc cgccgtcggc tccaagctcg
      301 gtagggtact cggtccgcgc ggcctgctcc cgaaccccaa ggccggcacg gtcggccccg
      361 acatcggcgg catcgtgcgc gccctcaagg ccggtcagat cgagttccgc gccgacaaga
      421 cgggtgttgt gcacgccccg ataggcaagg cgagcttcgc ccccgcgcag atcgccgaga
      481 acttcgcggc cttgcgcagc gccgtcgagg ccgccaagcc ggaagccgcc cgcggccagt
      541 acgtcaagac catcttcctg acctcgacca tgggaccgag cgtgcggatc gccctcgggg
      601 ccgtgacaga ggcctgactt taggcatccc ggtgcgcagc gcaagccgag cacctctggc
      661 gttggagaca gcaggggccg ccaggcttaa agatcctgcc gaggcgttgg gaaccgtaac
      721 gcacctagcg ttcgggaccg gtacgcgctt cgcggcctgt tcaagggtag ctcgcaaggc
      781 gcccgagaac gtagttcgag aataggaagg tttcccatgg ccaaccccag gaacgtgacc
      841 gcggtcgcgg agctgcgcga gcagctcggc gacgccgaga cgttcttcct ggtggactac
      901 cagggactct ccgctggcga cctcggtcgg ctgcgcgcgg ctgtgcgctc ggccggcggg
      961 aggatgctcg tcgcgaagaa caccctcatc aacgtcgtgc tcaaggagca gggcatcgag
     1021 gggttcgagg cgacgttgca aggaccgacg gccctcgtgc tcgtcggcga cgagctcgtg
     1081 gcgcccgtca aggcgatcac cgagttcgcc aaggctcacc ccaaggagct gcccaagtcc
     1141 aagggcggcc gcctcgaggg gagtgccctc ggcgaggacg cgctcgtccg catcgccaag
     1201 ctgccttcga agcagcagat ccagagccag ttggtcggcg tgctcatcgc gccgc
//
LOCUS       DAOQYE010000803         1254 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_143556_length_1254_cov_1.721435, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000803 DAOQYE010000000
VERSION     DAOQYE010000803.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1254
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143556_length_1254_cov_1.721435"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..877
                     /locus_tag="PKN52_11270"
     CDS             <1..877
                     /locus_tag="PKN52_11270"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNR00577.1"
                     /translation="VGPASAPVVYYLHGGPGYSAHSFRELVGDDLARWLVVYADMRGG
                     GRSFSDDGRGADPEALAADVPVVLDALGLESAVLLGHGFGALVAVRAALASPERVAGL
                     VLVNPWLSLPLLAERLLRTATAAAGPGDGSGADDLAGASGVRSDDDDPAGGAHGGPGL
                     GGAATADAAFRLMNPRILFDTLQFPNPSARLHLEHVDAEALLGPQEEDEADNVWESEA
                     LSALSRLAAGGKRVVVVAGELDGTTYPEQVEAALERLPNALYSGLRTGHYPWLDDPEA
                     FAATLEAALAYVTGA"
     gene            966..>1254
                     /locus_tag="PKN52_11275"
     CDS             966..>1254
                     /locus_tag="PKN52_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00578.1"
                     /translation="MLQPDARVASVREVTPQWLAARGLEGLLVDLDDTLLAARSERVD
                     EEVYAWFGSLRAAGVRVAILSNGETGRVASIAAQAGVPAVAMAGKPFARAFR"
BASE COUNT          152 a          455 c          458 g          189 t
ORIGIN      
        1 cgtcggaccg gcctccgccc ccgtcgtcta ctacctgcac ggcggtcccg gctacagcgc
       61 gcactcgttc cgcgagctcg tcggcgacga cctcgcgcgc tggctggtcg tctacgccga
      121 catgcgcggc ggtggccgct cgttctcgga cgacggccgc ggcgccgacc cggaggcctt
      181 ggccgccgac gtgcccgtgg tgctcgatgc ccttggtctg gagagcgccg tgcttctggg
      241 gcacgggttc ggcgcgctgg tggccgtgcg cgcggccttg gcgtcgcccg agcgcgtcgc
      301 aggcctcgtg ctcgtgaacc cctggctgtc gctcccgctg ctcgccgagc gcctgctccg
      361 cacggcgacc gccgccgccg gacccggtga cggctctggc gccgacgacc tggcaggcgc
      421 gtcgggcgtc cggtcggacg acgacgatcc agccggcggc gcccacggcg ggccggggct
      481 cggcggcgcc gcgaccgcgg acgccgcctt ccgcctgatg aacccgcgga tcctcttcga
      541 cacgctccag ttccccaacc cgtccgcccg gttgcacctc gagcatgtcg acgccgaagc
      601 cctcctcgga ccgcaggagg aggacgaagc ggacaacgtg tgggagtcgg aggcgctgtc
      661 ggcgttgagc cgcctggcgg ccggcgggaa gcgggtcgtg gtcgtggcgg gcgagttgga
      721 cggcacgacc tacccggagc aggtcgaagc cgccctcgaa aggctgccga acgcgctata
      781 cagcgggctg aggacgggcc actacccgtg gctcgacgat cccgaagcgt tcgctgcgac
      841 gctcgaggcg gccctggcgt acgtcacggg cgcctgacgc cggccatggt caggcgccta
      901 gccgcgcctg gtccacgtga cttcttactc tcgccgcccc gcggtctgcg tgctagtctc
      961 cccaagtgct ccaaccggac gcccgtgtcg cctccgttcg cgaggtgacg ccgcaatggc
     1021 tggctgcgcg tggcctcgaa gggctactgg tggatctgga cgacaccttg ctcgccgcgc
     1081 gctccgaacg ggtcgacgaa gaggtctatg cgtggttcgg cagtttgcga gcggcaggag
     1141 tgagagtggc tatcctctcg aacggcgaga caggcagggt ggcgagcatc gcggcgcagg
     1201 ccggggtacc cgccgtcgcc atggctggca agcccttcgc gcgggcgttc cggc
//
LOCUS       DAOQYE010000804         1253 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_143620_length_1253_cov_3.299666, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000804 DAOQYE010000000
VERSION     DAOQYE010000804.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1253
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143620_length_1253_cov_3.299666"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1229)
                     /locus_tag="PKN52_11280"
     CDS             complement(<1..1229)
                     /locus_tag="PKN52_11280"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNR00579.1"
                     /translation="MRVLQDHDLPETLRLAQLAFETGYAQVEVVTQYAHLLARSGRKA
                     DMERLLASLPAKAMTPDRRASLLVTSRNVAGEHQEAWTTWLGHPELDADPAPELLRAV
                     AASALAVGRVAEAKVLTRRGLEAATTDGLRCEFLSILSLIAYHEGDAAGANEHIGQAL
                     ALLSTLHAPRLRATALVNRAAYLRMLGDYEGMESCLEECLRIRQGVGEGMSYAFALAS
                     LADLRIEQGRLEEAEDMVTEAITTLELYGPSRYLNNTRSIACLLGLAQATPLSRLTAL
                     KHGEQALAAARAVGNPRAVRELLFDASLANTATGNAERGLALAKESLALADATGNAPT
                     DAYRTLWALALAREALGEASVALELLLQAEAIAGDTEGAVDFHKVGLEIARIRGDVEA
                     ARDHQAWFEARDLLSGAT"
BASE COUNT          184 a          427 c          433 g          209 t
ORIGIN      
        1 gttgctccgc tcaagagatc gcgcgcctcg aaccaggcct ggtgatccct cgcagcttcg
       61 acgtcaccgc gtatacgcgc gatctcgagt cctaccttgt ggaagtcgac ggccccttcg
      121 gtgtcgccag cgatggcttc ggcttggagc aacaactcga gcgctacgct cgcttccccc
      181 agcgcttccc gcgcgagcgc gagcgcccac agcgtacggt aggcgtcggt gggggcgttg
      241 cccgtagcgt cggcgagcgc gagggactcc ttcgccaacg cgagcccgcg ctcggcgtta
      301 ccggtcgcgg tgttggcgag cgaggcgtcg aacaagagtt cgcgcaccgc gcgcgggttc
      361 ccgacggcgc gggcggctgc caacgcctgc tcgccgtgct tgagcgccgt caagcggctg
      421 agcggggtcg cctgcgcgag gccgagcaag catgcgatgg aacgggtgtt gttgaggtag
      481 cggctgggtc cgtacagctc caaggtagtg atggcctcgg tgaccatgtc ctccgcttcc
      541 tcgaggcggc cctgttcgat ccgtagatcg gcgaggctgg cgagcgcgaa ggcgtaagac
      601 atgccttcgc ctacgccctg ccggatccgc aggcactcct ctaggcacga ttccatgcct
      661 tcgtagtcgc cgagcatgcg gaggtaggcg gctcggttga ccagggccgt cgcgcgaagg
      721 cgcggcgcat gcaacgtcga gaggagagcg agtgcttggc cgatgtgctc gttggcgccc
      781 gcggcgtcgc cttcgtggta cgcgatgagc gacaggatcg agaggaactc gcagcgcagc
      841 ccgtccgtcg tggccgcctc caagccgcgc cgcgtcagga ccttggcttc ggcaacgcga
      901 ccgaccgcca acgccgaggc cgctaccgcc cgcagcagtt ccggtgccgg atccgcgtcg
      961 agttcgggat gccccagcca ggtcgtccac gcttcctggt gctccccggc cacgttgcgg
     1021 ctggtgacca gcaaggaagc ccggcgatct ggagtcatcg ccttggctgg cagcgaggcg
     1081 agcaaccgct ccatgtccgc cttccggcct gagcgcgcca ggaggtgggc gtactgcgtc
     1141 accacctcga cctgcgcgta gccggtctcg aacgccagct gcgccaaccg tagggtctcg
     1201 gggaggtcat ggtcctgcag caccctcatg gcctcgactg ctttccggca acg
//
LOCUS       DAOQYE010000805         1252 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_143858_length_1252_cov_2.440267, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000805 DAOQYE010000000
VERSION     DAOQYE010000805.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1252
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143858_length_1252_cov_2.440267"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="PKN52_11285"
     CDS             complement(<1..462)
                     /locus_tag="PKN52_11285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HNR00580.1"
                     /translation="MARPRTALPLLVMAALFAGCTPAAMRAESRAPDARTTAPAVAAT
                     AATSAGAQTGVASWYGPGFAGRRTANGEVFDPSQLTAAHRELPFNTLVRVVNENNGKS
                     VVVRINDRGPFKEGRVIDLSRAGAEAIDMIGSGVARVRLEVLSLPAGVVRVG"
     gene            complement(577..750)
                     /locus_tag="PKN52_11290"
     CDS             complement(577..750)
                     /locus_tag="PKN52_11290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00581.1"
                     /translation="MVHQFARGNDDVRYLSNEELSEIISKCEDAIQDGSAEVEDYEAF
                     VVCQKELARRTWA"
     gene            968..>1252
                     /locus_tag="PKN52_11295"
     CDS             968..>1252
                     /locus_tag="PKN52_11295"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNR00582.1"
                     /translation="MTDSHCHLDRCPDPEAAVDAGLAAMVTVGTDVERSRLAVAAAHA
                     HENVYAAVGVHPNDASLAADAAVRDAIARLALDPLVVAVGETGFDRYWDDE"
BASE COUNT          188 a          429 c          447 g          188 t
ORIGIN      
        1 gccgacacgg acgacgccgg ccggcaggct gaggacctcg aggcggacgc gcgcgacccc
       61 ggaaccgatc atgtcgatgg cctctgcgcc ggcgcgcgag aggtcgatga cccgcccctc
      121 cttgaagggg ccgcggtcgt tgatgcgcac gacgacgctc ttgccgttgt tctcgttgac
      181 gaccctgaca agcgtgttga acggcaactc gcgatgggcc gctgtgagtt ggctagggtc
      241 gaacacctcg ccgttggcgg tgcgccgccc ggcgaacccg gggccgtacc aggaagctac
      301 ccccgtctgc gcgccagcgc tggtcgccgc cgtggcggcg acggccgggg cggtcgtacg
      361 cgcgtcgggc gcgcgcgact cggcgcgcat ggcggccggc gtgcagcccg cgaacagggc
      421 ggccatgacg aggagcggca gggccgtccg gggcctggcc acggcacagc cgtgaccaca
      481 cgtagaagcg cgcgtgagca taggccatgc tacgcgcgct tcatgtcgtt gtgatgtgag
      541 gaaccgggcg gcggtaccgc ggggccgtgg tagggctcac gcccaggtgc ggcgggcgag
      601 ctccttctgg cacacgacga acgcctcgta gtcctcgacc tcggcgctgc cgtcctggat
      661 ggcgtcctcg cacttggaga tgatctcgct aagctcctcg ttgctcaggt accttacgtc
      721 gtcgttaccg cgagcgaact ggtgaaccat ggtgcttgcc taccttcctc gagccttcga
      781 actctcttgc tcgccgggtc ctcgtcgaag acccgccccc ggtcaagtga tgagaaggta
      841 gcagcgggcc tattacagaa gtctcacatg gggttcacgc cagcgccgtg tggcacggcg
      901 ctctcaccga ccgcgtcgcg ttggcgccga cgcagccgcg aagaaagccg agcggtagag
      961 tgggggcgtg acggatagcc actgccacct cgatcgctgc cctgaccccg aggccgccgt
     1021 cgacgccggc ctcgccgcca tggtgacggt aggcacggac gtcgagcgca gccggttggc
     1081 cgtcgcggcc gcgcatgcgc acgagaacgt ctacgccgcc gtcggcgtcc acccgaacga
     1141 cgcgagcctc gccgccgacg cggcggtaag agacgccatc gcgaggctcg ccctcgaccc
     1201 gctggtggtg gccgtcggcg agacgggttt cgaccggtac tgggacgacg ag
//
LOCUS       DAOQYE010000806         1251 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_144018_length_1251_cov_3.127926, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000806 DAOQYE010000000
VERSION     DAOQYE010000806.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1251
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144018_length_1251_cov_3.127926"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="PKN52_11300"
     CDS             <1..370
                     /locus_tag="PKN52_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HNR00583.1"
                     /translation="ISKEPLGPAVLHGDDQWYDVVSWTIYALFDAEEYGVTQANVDEM
                     LVNAADPQVKRLLGAPGSESYVEAFGLRADAFYQVIKAVGNYGEIFDRNLGSGSVFGL
                     ERGINAQYYDGGLIYGYPFN"
     gene            540..>1251
                     /locus_tag="PKN52_11305"
     CDS             540..>1251
                     /locus_tag="PKN52_11305"
                     /inference="COORDINATES: protein motif:HMM:TIGR01726.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNR00584.1"
                     /translation="MQPGKPSMSASTAVVPFWRNVKIIGVIAQFVFVIALVAGVGILV
                     NNVVTALAKANLPADFSFLGKPAGIPIAERAVPYQVTDTYARALLIGFLNTLKVAVVG
                     VVLATVIGVLMGVMRLSSNWLVRTMASVYVEVLRNIPLAVQIVFWYSAVLLPFPPRIS
                     DPIALPGGLIMSNVGLAFPFLYPTYRFGVWVPYLIAAAVLACVAWWWRRRALAKADRV
                     GSVWPFPVVTFLVVAGLGL"
BASE COUNT          188 a          441 c          391 g          231 t
ORIGIN      
        1 catctccaag gagccgctcg gcccggccgt cctgcacggt gacgaccagt ggtacgacgt
       61 cgtctcctgg accatctacg ccctgttcga cgccgaagag tacggcgtga cgcaggcgaa
      121 cgtcgacgag atgctcgtca acgccgccga cccgcaggtg aagcgtctcc tcggcgcccc
      181 cggctccgag tcctacgtcg aggccttcgg cctccgcgcc gacgccttct accaggtgat
      241 caaggcggtc ggcaactacg gcgagatctt cgaccgcaac cttggctccg gctccgtgtt
      301 cggcctcgag cgcggcatca acgcccagta ctacgacggc ggcctcatct acgggtaccc
      361 gttcaactga gcgcgacttg aatcgagggc agcggcgccc ttcggggcaa cacccgaacg
      421 gcgccgctcc gctctcgctc gtccgggagc ggcgccgacc gctcgacctt acgaggacgg
      481 cggccccggt gggaaagacc atgacgacag actgccaaag aacaggacct tgaggacgca
      541 tgcagccagg taagccgagc atgagcgcgt cgacggcagt cgtgcctttc tggcgcaacg
      601 tcaagatcat cggggtcatc gcgcagttcg tgttcgtcat cgcgctcgtc gcgggcgtcg
      661 gcatcctcgt caacaacgtc gtcacggcct tggcgaaggc caacctgccg gccgacttct
      721 ctttcctcgg caagccggct ggcatcccca tcgccgagcg ggccgtcccg taccaggtga
      781 ccgacaccta cgctcgggcc ctcctcatcg ggttcctcaa cacgctgaag gtcgccgtag
      841 tgggcgtcgt gctcgccacc gtcatcggcg tcctcatggg cgtgatgcgt ctatctagca
      901 actggctcgt ccggaccatg gcgagcgtat atgtggaagt cttgcgcaac atcccgctcg
      961 cggtgcagat cgtcttctgg tactccgcgg tgctgctccc gttcccgccg cgcatcagcg
     1021 atccgatagc gctgcccggc ggtttgatca tgtccaacgt cggcttggcc ttcccgttcc
     1081 tgtaccccac gtaccggttc ggagtgtggg tgccgtacct gatcgccgcc gcggtgctcg
     1141 cctgcgtcgc gtggtggtgg cgtaggcgcg cgctcgccaa ggccgacagg gtcggaagcg
     1201 tctggccgtt ccctgtcgtc acgttcctgg tggtcgccgg ccttggcctc g
//
LOCUS       DAOQYE010000807         1247 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_144840_length_1247_cov_2.385067, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000807 DAOQYE010000000
VERSION     DAOQYE010000807.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144840_length_1247_cov_2.385067"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..593
                     /locus_tag="PKN52_11310"
     CDS             <1..593
                     /locus_tag="PKN52_11310"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNR00585.1"
                     /translation="PNASLDDGLLELSRAALNDEWYGGLKRRIVCVARLSAQKDHATL
                     LRAFADLGEGEGTGLVLIGAGPLEAEVRELVDSLGLTGRVKMVTSEANPFRWLRNADL
                     LVLASRWEGSPNVLVEAMALGVPVVATDCPSGPREILAGGEFGELVPVGDSAALAEAM
                     RRTLARPRDARRLVERSRAFHIESVGPRWLAALLSR"
     gene            590..>1247
                     /locus_tag="PKN52_11315"
     CDS             590..>1247
                     /locus_tag="PKN52_11315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00586.1"
                     /translation="MTIRFRPRAVLEWSAAFVYVLLLSGALLPPLLAPGGSGTVDLDA
                     GLPALQAAMVAFLAIVGALFCLHFWRRPVVPLLKAAWLPLALATLAVASILWSVNQEL
                     TARRSLALLGTVLIAAYLVGVLGYKRSVSLLTILLAVVALLSLVVVVATPDFGVHQVG
                     AHVGRWKGVYLHKNLLGREMALAGGLFTLAAIHARRPSARLFWAAMTVLAVVLIVQAQ
                     S"
BASE COUNT          152 a          403 c          453 g          239 t
ORIGIN      
        1 tgcccaacgc gagcctggac gatggtctgc tcgagctgtc gcgagccgcg ctgaacgacg
       61 agtggtatgg cggtctgaag agacggatcg tgtgcgtcgc caggctgtcc gcgcagaagg
      121 accatgccac gctcttgcgg gctttcgccg acctcgggga gggggagggg accggcctgg
      181 tgctgatcgg ggcaggtccg ctcgaggcgg aagtgcgcga gctggtcgac tcgctgggtc
      241 tgaccgggcg cgtgaagatg gtaacgagcg aggcgaaccc cttcaggtgg ctgaggaacg
      301 ccgacctgct ggtcctggca tcgcgctggg aaggttcacc gaacgtgctc gtcgaggcta
      361 tggcgctggg tgtgccggtg gtcgccacgg attgcccatc cggtccacgc gagatcctgg
      421 ctggcggcga gttcggcgag ctggtcccgg tgggcgactc cgccgcgctg gccgaggcga
      481 tgcgtcggac actcgcgcgc ccgcgcgacg ctcgccggct ggtcgagcgt agccgggcct
      541 tccatatcga gagcgtggga ccgcgatggc tggcggcgct gctttcgcga tgaccatccg
      601 cttccgtcca cgggccgtct tggagtggag cgcggcgttc gtttacgtgc tgctcctgtc
      661 cggggccctg ctgccgcctc tcctggcccc tggcggttcg ggaaccgtcg acctcgatgc
      721 cggcctgccg gccttgcaag cagccatggt cgctttcctg gcgatcgtcg gcgcgttgtt
      781 ctgcctccat ttctggcgga ggccggtcgt ccccctgttg aaggccgcct ggctgccgtt
      841 ggccctcgcc acgctggctg tcgcgtccat cttgtggtcg gtcaaccaag agctgaccgc
      901 taggcgctcc ctggcgctcc tcggcacggt cctgatcgcc gcgtacctag tgggcgtgct
      961 cggctacaag cgcagcgtct cgctgctcac catcctgctg gcggtggtcg ccctgttgtc
     1021 gctggtcgtg gtcgtggcca cgccggactt cggcgttcac caggtaggcg cccacgtcgg
     1081 acgttggaaa ggtgtgtacc tgcacaagaa cctcctcggc agggagatgg cgctggccgg
     1141 agggctgttc actctcgccg ccatacatgc gaggcggccg agcgcgcgct tgttctgggc
     1201 agccatgacg gtgctggccg tcgtactgat cgtgcaggcc cagtcgg
//
LOCUS       DAOQYE010000808         1246 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_144970_length_1246_cov_3.434089, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000808 DAOQYE010000000
VERSION     DAOQYE010000808.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144970_length_1246_cov_3.434089"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1149
                     /locus_tag="PKN52_11320"
     CDS             <1..1149
                     /locus_tag="PKN52_11320"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNR00587.1"
                     /translation="GVLQRLSPTEHLTPTLGDRLRKQGERLAVAACSTPGASLLWNPK
                     NPQRVLNASTDYGDADLAAVRDKLGPVGPDVGRTKYNACRWATRALIDVLLDQPENRV
                     ITLWLTEPDASQHFYGLGSPEALEALTVVDECVGMVVDALARKGLADEFDLLLVSDHG
                     HSSVRHSGSLAEHLIEARDALDLGDLIAVADFIYSPTGDHDPSALPRLADWLLERPWC
                     GLLFTSVPAMYGHRGVLPLELATGPLSHGRAPLLAVSPTWSHAVNAFGVPGSVDTLTS
                     YSPLKSTHGTASPYDMRAFCLGVGPRFERGARIDVPCGTVDIAPTVWDLIGNDAPSAS
                     YGFDGGSLLTLGAGTPVRRERVAAESSREAIEVAHVDGRSYFLGSVKP"
BASE COUNT          186 a          462 c          411 g          187 t
ORIGIN      
        1 ggcgtattac aacgactcag cccgacagag catctcaccc cgacgctcgg cgatcgactg
       61 cgcaagcaag gggagcgcct ggccgtggcg gcgtgcagca cgccgggcgc gtcgctactc
      121 tggaacccca agaacccgca gcgcgtcctc aacgccagca ccgactacgg cgacgcggac
      181 ctcgccgccg tgcgcgacaa gctcggcccg gtcgggcccg atgtcgggcg taccaagtac
      241 aacgcctgcc gctgggccac cagggccctc atcgacgtgc tgctcgatca gccggagaac
      301 cgcgtcatca ccttgtggct cacggagccg gacgccagcc agcacttcta cggtctcggc
      361 tcacccgagg cgctcgaggc cctgacagtc gtggacgagt gcgtcggcat ggtggtggac
      421 gccctcgccc gcaaggggct cgccgacgag ttcgacctgc tcctcgtgag cgatcacggc
      481 cactcgtccg ttcgccactc cggctcgctc gccgagcacc tgatcgaggc gcgcgacgcg
      541 ctcgaccttg gcgacctcat cgcggtggcc gacttcatct actccccgac cggcgatcac
      601 gacccgagcg cgttgccgcg cctggccgac tggctgttgg agcgcccgtg gtgcggcctg
      661 ctcttcacgt cggtgccggc catgtacgga caccgtggcg tgctgccgct cgagctggcg
      721 accggcccgc tgtcgcacgg cagggcgccc ctcctggccg tcagcccgac ctggagccac
      781 gccgtcaacg cgttcggcgt gcctggcagc gtcgacacgc tgacctcgta ctcgcccctc
      841 aagtccaccc acggcaccgc ctcgccttac gacatgcgcg cgttctgcct gggggtcggg
      901 ccgaggttcg agcggggtgc ccgcatagac gtgccgtgcg gcaccgtcga catcgctccg
      961 accgtgtggg acctcatcgg caacgacgcc ccctcggcca gctacggctt cgacggcggc
     1021 tcgctactca ccctcggggc tgggacgccc gtacgccgcg agcgtgtggc ggccgagtcg
     1081 agcagggagg cgatagaggt cgcgcatgtc gacggcagga gctacttcct cggaagcgtg
     1141 aagccctagc gcgaacgaca acgcgaacgc cggggtccca tgtgctggaa ccccggcgtc
     1201 aggcggtgat tctcgacgcg tcgagcgcct ttagtcgatg acctgg
//
LOCUS       DAOQYE010000809         1246 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_144997_length_1246_cov_2.814442, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000809 DAOQYE010000000
VERSION     DAOQYE010000809.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144997_length_1246_cov_2.814442"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="PKN52_11325"
     CDS             <1..472
                     /locus_tag="PKN52_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNR00588.1"
                     /translation="TLAIPSGVPPIENIMLAIAVTAWPSYARLMRGEVLRIKEEEYVA
                     GARAIGRRPLGIIGRHVLPNAIGPIVIVASLDIGSVVLTAAALSFLGLGAEIGYADWG
                     QMISFARDWILGPPGKPFYFWYVSFWPGLIILLFVLAWNLLGDAFRDVLDPQSK"
     gene            complement(486..1049)
                     /locus_tag="PKN52_11330"
     CDS             complement(486..1049)
                     /locus_tag="PKN52_11330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNR00589.1"
                     /translation="MQLAFASLPLVAVVGAILGTALSTTAGASAGSATPADYDAAIYG
                     AAIADGATLYALNCAVCHGKAAGGLADAKLSFPPEERTCTRCHRPNNRTVQSLSEPVV
                     DNDMFAIGTPPSLVATPAHPQPLALVAEPAALWAYVSATMPRYEPGRQTPAEYWLLTA
                     YLLDLNGRSEAAAEAVANAAAPVTKQP"
BASE COUNT          154 a          426 c          453 g          213 t
ORIGIN      
        1 gacgctcgcc attccgagcg gagttccacc gatcgagaac atcatgctcg ccatcgccgt
       61 taccgcgtgg ccgtcctacg cccgcctcat gcgtggcgag gtgctgcgca tcaaggagga
      121 ggagtacgtg gccggcgcca gggccatcgg gcggcggccg ctcggcataa tcggcaggca
      181 cgtgctgccg aacgccatcg ggccgatcgt gatcgtcgcc agcctcgaca tcggctccgt
      241 agtgctcacc gccgccgcgt tgtcgttcct cggcctgggc gccgagatcg gttacgccga
      301 ctggggccag atgatctcgt tcgcgcgcga ctggatcctc ggcccgcccg gcaaaccgtt
      361 ctacttctgg tacgtctcgt tctggcccgg cctgatcatc ctgctcttcg tgctggcctg
      421 gaacctgctg ggcgacgcgt tccgcgacgt tctggacccg cagtcgaagt gagggctccg
      481 ccccgtcatg gctgcttggt cacgggtgcg gccgcgttgg ctaccgcctc cgcggccgct
      541 tcgctcctgc cgttgaggtc caggaggtag gcggtcagga gccagtactc ggccggcgtc
      601 tgacggcccg gttcgtagcg cggcatcgtg gcgctcacgt aggcccataa ggcggccggt
      661 tccgcgacga gggccagggg ctgcgggtgc gccggcgtgg ccaccaggga cggcggcgtg
      721 cctatcgcga acatgtcgtt atcgaccacg ggctcggaca gcgactgcac cgttcggttg
      781 ttcgggcggt ggcagcgagt gcacgtgcgc tcctcgggcg ggaaggagag cttggcgtcc
      841 gccaggcccc cggcggcctt gccgtggcat acggcgcagt tcagcgcgta gagggtggcg
      901 ccatccgcta tggctgcgcc atatatggcg gcgtcatagt ccgcgggcgt ggcggagccg
      961 gcgctcgcgc ccgccgtggt gctcagggct gtgccaagga tcgcgccgac caccgccacc
     1021 aacggcaggc tcgcgaacgc gagctgcacg ctgcagcgcc gggggctcca ccacctcagg
     1081 gataggggac gctcctccag cgcagccgca gcgcagccgt cttcaggcgc cttcgtcggt
     1141 gggttcgtcg ttggcttccg aggcttcggc cggctcggtg gcgctcgggg tgaccgcagg
     1201 cgctgcctcg gtcggggcga cggcggcttc ctgggcgacg gcggct
//
LOCUS       DAOQYE010000810         1245 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_145174_length_1245_cov_2.845378, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000810 DAOQYE010000000
VERSION     DAOQYE010000810.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1245
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_145174_length_1245_cov_2.845378"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="PKN52_11335"
     CDS             <1..454
                     /locus_tag="PKN52_11335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00590.1"
                     /translation="HAAEVIADFDAYVAAAKIQIHVPAGTANIISFNGAGTTNPIGRA
                     VGPHAQLLSSLGFVIEDPDVAWHTQASLRNDFVWAEFERLTDLTSETTFLLRFGPDGV
                     ASFVSEPVLANMPSVKAGQVYGLGVNSFRIDYYSAIEIVDSIVAEFGN"
     gene            463..>1245
                     /locus_tag="PKN52_11340"
     CDS             463..>1245
                     /locus_tag="PKN52_11340"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HNR00591.1"
                     /translation="MARRPLVVVASVAVLVVLALLSFGVGTKPLAPSTTWDAIFAFDP
                     ANSDHLLVRYLRLPRTALAVVVGCCLGVAGTVMQALTRNALADPGILGVNAGAAAAIA
                     AAIAFFGVTSVTSYMWFGLLGAAVAGVAVYFLGGMRRGSSPVRLVLAGAALSIVLLAL
                     TQVVIVNSEETVFDQFRHWTVGSLQGRGTSVVLPVTLLALVGVATSFVLARALNAAVL
                     GEEVARSLGANPVRVWSLAALAVILTSGAATAAAGPVGFVGLT"
BASE COUNT          162 a          462 c          411 g          210 t
ORIGIN      
        1 acacgccgcc gaggtcatcg cggacttcga cgcctacgtc gcggccgcca agatccagat
       61 ccacgtgcca gccggtaccg ccaacatcat cagcttcaac ggcgccggca ccaccaaccc
      121 catcggccgc gccgtcggcc cccacgccca gctcctcagc agcctcggct tcgtcatcga
      181 ggacccggac gttgcctggc acacccaagc ctcgctccgc aacgacttcg tgtgggccga
      241 gttcgaacgc ctgacggacc tcacctcgga gacgacgttc ctcctgcgct tcggacccga
      301 cggcgtcgcc tcgttcgtgt ccgagccggt gctcgccaac atgccgtccg tgaaggccgg
      361 ccaggtgtac ggtcttggcg tgaactcgtt ccgcatcgac tactacagcg ccatcgagat
      421 cgtcgacagc atcgtcgccg agttcggtaa ctgaacagcg ccgtggcgcg ccgcccgctc
      481 gtggtggtcg ccagcgtggc agtcctggtg gtgctcgccc tgctcagctt cggggtgggc
      541 accaagccgc tcgcgccgag caccacctgg gacgccatct tcgccttcga ccccgccaac
      601 tcggaccacc tcctcgtcag gtacctgcgc ctgccgcgca cggcgctcgc cgtggtggtg
      661 ggctgctgcc tcggggtggc tggaaccgtc atgcaagcgc tcacgcgcaa cgcgttggcc
      721 gacccaggca tcctgggcgt caacgccggc gcggccgccg cgatcgcggc ggccatcgcg
      781 ttcttcggcg tcacgagcgt cacgagttac atgtggttcg gccttctcgg cgctgccgtg
      841 gccggggtgg ccgtgtactt cctcggcggc atgcggcggg ggtcgagccc cgtgaggcta
      901 gtgctggcgg gcgccgcgct ctcgatcgtc ctgctggcgc tgacgcaggt cgtcatcgtc
      961 aacagcgagg agacggtgtt cgaccagttc aggcattgga cggtaggctc gctgcaagga
     1021 cggggaacca gcgtcgtgct gcccgtgacc ctgttggcgc tcgtcggggt cgcgacgtcg
     1081 ttcgtcctgg cgcgggcgct caacgccgcc gttctcggcg aggaggtcgc gcggtcgctc
     1141 ggcgccaacc cggtcagggt ctggagcctc gccgccctcg ccgtgatcct cacgagcggc
     1201 gcggccacgg ccgccgctgg ccccgtcggc ttcgtcggcc tcacg
//
LOCUS       DAOQYE010000811         1244 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_145411_length_1244_cov_2.518923, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000811 DAOQYE010000000
VERSION     DAOQYE010000811.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1244
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_145411_length_1244_cov_2.518923"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..688
                     /locus_tag="PKN52_11345"
     CDS             <1..688
                     /locus_tag="PKN52_11345"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNR00592.1"
                     /translation="VGVTNIALALFNLLPAIPLDGGRVLRSVLALALTRERATAIATS
                     VSAVMAVLLGLYGFFTMQLWLVILAFFIYNAGRLEAQASVVASAFEGRTVADLMTRDP
                     VTVTPDMRLAQFAQLIHFRPHTGYPVVDPEGKLLGFARLAASGRPGVVGADAVDVDAT
                     AAVADITEPADTISPTASAYDALRRVAEGHLGRLVVVDGDERVVGLLSKADLVRTLSR
                     PEATRDGRRV"
     gene            722..>1244
                     /locus_tag="PKN52_11350"
     CDS             722..>1244
                     /locus_tag="PKN52_11350"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNR00593.1"
                     /translation="MLTALTFALILTSAVVVHELAHFLAARSVGVAVRAFSVGIGPIL
                     ARAKWRGTEWRLSLLPLGGYVDLPGMGPRVDEDGTLHHPDEGMAKLRLLPKLWVLVAG
                     VIANYVLGSVLLASAITMQPGFREITGAPVVVEGSRIARVVPGSAAEGLGIRSGDLVL
                     SLNGLANPDPDAVV"
BASE COUNT          173 a          446 c          420 g          205 t
ORIGIN      
        1 cgtcggggtc accaacatcg ccctggccct cttcaacctc cttccagcca tcccgctcga
       61 cggcggccgc gtcctcagga gcgtcctcgc gctggcgctg acccgcgagc gcgccaccgc
      121 catcgccacg tccgtaagcg ccgtcatggc cgtgctgctc ggactgtacg gcttcttcac
      181 catgcagctc tggctggtca tcctggcgtt cttcatctac aacgcgggcc gactagaggc
      241 tcaggcctcc gtggtggcga gcgcgttcga ggggcgcacc gtcgccgacc tgatgacgcg
      301 cgaccccgtc acggtgacgc ccgacatgcg cctggcccag ttcgcgcagc tgatccactt
      361 caggccgcat accggctatc cggtcgtcga cccggaaggc aagttgctcg ggttcgcgcg
      421 cctggctgcg tccgggcggc ccggggtagt tggagcggac gccgtcgacg tcgacgccac
      481 ggccgctgtc gcggacatca cggagccggc cgacaccatc tccccgacag ccagcgcgta
      541 cgacgcgctg agacgcgtcg ccgaaggcca tctgggacgt ctggtggtcg tcgacggcga
      601 cgagcgggtc gtcgggctgc tcagcaaggc cgacctggtg agaacactct cgcgaccgga
      661 agcgactcgc gatggccggc gggtctgaca tactgcctgt tagcctctgg agcgaggcgc
      721 gatgctgact gccctgacct tcgcactgat cctaacgagc gccgtcgtcg tgcacgaact
      781 ggcgcacttc ctcgccgccc gcagcgttgg cgtggccgtg cgcgccttca gcgtcggcat
      841 aggccccata ctggcgcggg ccaagtggcg cggcacggag tggcgcctca gcctgctgcc
      901 gctggggggc tacgtcgacc tgcccggcat gggaccgcga gtcgacgagg acggcacgct
      961 ccatcatcct gacgagggga tggccaaact gcgcctcctc cccaagctct gggtcctggt
     1021 ggcgggagtg atcgccaact acgtgttggg cagcgtcctt cttgcgtccg ccataaccat
     1081 gcagccgggc ttccgcgaga tcacgggcgc accggtggtc gtggagggtt cgcggatcgc
     1141 ccgcgtcgtg cctggctccg ccgcggaggg gctcggcatc aggagcggcg atctcgtcct
     1201 gtcgctgaac ggcctcgcca accccgaccc cgacgcggtg gtgg
//
LOCUS       DAOQYE010000812         1242 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_145743_length_1242_cov_3.673126, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000812 DAOQYE010000000
VERSION     DAOQYE010000812.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1242
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_145743_length_1242_cov_3.673126"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            112..984
                     /locus_tag="PKN52_11355"
     CDS             112..984
                     /locus_tag="PKN52_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNR00594.1"
                     /translation="MSATTRMTTSPRIKNKALRRFLRNPGTVLGSILTILLVAVALLA
                     PVIAGDPLLQNLAERLKPPSAQHLLGTDQLGRDVWARVVHGSRISLRIGLTVTGIALV
                     LGGLLGLLAGMLGGLVDEIAMRITDIFFAFPALILAMAIAGALGPSLVNLMIAVAAVS
                     WPYYARLIRAQVLSLKSLDFVDASRALGASRWRQALRHVLPNAITPLLVQASFDVGGA
                     ILTAAGLGFIGFGAQPPTPEWGAVVSETRSFIIEAPWASSAPAVAILLTVLAFNLLGD
                     GLRDVLDPRGRRRG"
     gene            complement(1000..>1242)
                     /locus_tag="PKN52_11360"
     CDS             complement(1000..>1242)
                     /locus_tag="PKN52_11360"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HNR00595.1"
                     /translation="WLQYPKDAACADLTEQFFLGPDLLVAPVLREGATTVRAYLPAGS
                     GTWRHRWTGMRYEAGGGAWVEVSAPVGSPGLFDREG"
BASE COUNT          164 a          488 c          387 g          203 t
ORIGIN      
        1 cgacttcccc gccgtgatgg gcgtgacgct cgtggcgggt ctggcgtata gcctcatcaa
       61 cctactcgta gacgtcctgt acgccgtctt cgacccgagg atcgcctact cgtgagcgcg
      121 accacccgga tgaccacgag ccccaggatc aagaacaagg cgctgaggcg cttcctacgc
      181 aacccgggca cggtgctggg cagcatcctg accatactgc tcgtggccgt cgccctcctc
      241 gcccccgtaa tagcgggcga cccgctcctg cagaacctcg cagagcgcct caagccgccc
      301 agcgctcagc acctcctcgg cacggatcag ctcgggcgcg acgtctgggc gcgcgtcgtg
      361 cacggctccc gcatctcgct gcgcatcggt cttaccgtga ccggcatagc gctcgtcctc
      421 ggcggcctgc tcggactgct cgcgggcatg ctcggcggtc tcgtcgacga gatcgccatg
      481 cgcatcaccg acatcttctt cgccttcccg gccctcatcc tcgcgatggc catcgcgggc
      541 gcgctcgggc cgagcctcgt caacctcatg atcgccgtgg ccgccgtgtc gtggccgtac
      601 tacgcccgcc tgataagggc gcaggtgctc agcctcaagt cgctcgactt cgtcgacgcc
      661 tcgcgcgcgc tcggcgccag ccgctggcgc caggccttgc ggcacgtgct gcccaacgcc
      721 atcacgccgc tcctcgtcca ggccagcttc gacgtcggcg gggccatcct cacggccgcc
      781 ggcctcggct tcatcggctt cggagcgcaa ccgcccacgc cggagtgggg cgcggtcgtg
      841 tcggagacgc gctccttcat catcgaggcg ccgtgggcgt cttcggcacc ggccgtcgcc
      901 atcctcctga cggtcctcgc cttcaacctc ctcggcgacg gcctgaggga cgtgctcgat
      961 ccgcgcggtc gacggcgcgg ctgagcccct ccccggtcct cagccctcgc ggtcgaacag
     1021 gcccggactc cccaccgggg cgctcacctc tacccacgct cccccgcccg cctcgtagcg
     1081 catgcccgtc caccggtggc gccatgtgcc gctgccggcc ggcaggtagg cgcgcacggt
     1141 agtggcgccc tctctcaaca cgggggcgac gagcaggtcg gggccgagga agaactgctc
     1201 ggtcagatcg gcgcacgccg catccttcgg gtattggagc ca
//
LOCUS       DAOQYE010000813         1241 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_145942_length_1241_cov_2.990725, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000813 DAOQYE010000000
VERSION     DAOQYE010000813.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_145942_length_1241_cov_2.990725"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..940
                     /locus_tag="PKN52_11365"
     CDS             <1..940
                     /locus_tag="PKN52_11365"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF013296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HNR00596.1"
                     /translation="YAALVCAAAASTGYPGPVLIQGDHFAVESDDDASVVEVETLARS
                     ALGSGFLQVDLDAAALAAEGASAAARQDRNAAVTASLIARLAPHAPAGAIFGGEVGEI
                     GGANTTPEELDVFVDLVRRHAGAAAGAFGKVSVQTGTRHGGITDASGKPARMPLDTAL
                     ARELADVAMAKGLPGIVQHGASTLQGDQFAALPGCGVVEVHLATNLQDLVFDSPAFPP
                     ELRAELMAAALGGPARAAERGADTSGVGDQLFRQRRWSLWGPFKRRLLDLPDETRARL
                     AAGVADWAGDLMVAFGLAGRAAEIRGLYADAAASDR"
     gene            978..>1241
                     /locus_tag="PKN52_11370"
     CDS             978..>1241
                     /locus_tag="PKN52_11370"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNR00597.1"
                     /translation="MRWIGAVAMRMNAPILFLGMATLDFVTVVPRLPASDEVFEVRSL
                     SMHGGGPAATAAVACARLGGDARFAGALGRDPVAETVLAQLASE"
BASE COUNT          145 a          470 c          453 g          173 t
ORIGIN      
        1 gtacgccgcg ctcgtgtgcg cggccgccgc ctcgacgggc taccccggcc ccgtcctcat
       61 ccagggcgac cacttcgccg tcgagagcga cgacgacgcc agcgttgtcg aggttgagac
      121 gctcgcgcgc tcggcgctcg gcagtggctt cctccaagtc gatctcgacg ccgccgcgct
      181 cgcggcggag ggcgccagcg cggcggcgcg gcaggaccgc aacgccgccg tcaccgcgag
      241 cctgatagcc cggttggcgc ctcacgcgcc cgccggcgcg atcttcggcg gcgaggttgg
      301 cgagatcggc ggagccaaca ccacgccgga ggagctcgac gtgttcgtcg acctcgtgcg
      361 ccgccacgcc ggcgcggcgg ccggcgcctt cggcaaggtg agcgtccaga ccggcacgcg
      421 ccacggcggc atcaccgacg cgtcgggcaa gccggcccgc atgcccctag acaccgcgct
      481 ggcgcgcgag ctcgccgacg tcgccatggc caaggggctc cccggcatcg tccagcacgg
      541 tgcctcgacg ctccagggcg accagtttgc tgccctgccg ggctgcggcg tcgttgaggt
      601 gcatctggcg acgaacctgc aggacctcgt cttcgactcg cccgccttcc cgcccgagct
      661 gcgcgccgag ctgatggctg ccgccctggg tggtcccgcc cgagccgccg aacgcggagc
      721 ggacacctcc ggcgtgggcg accaactctt caggcagcgg cgttggagcc tctggggccc
      781 gttcaagcgc cgcctcctcg accttcccga cgagacccgc gcgagactgg ccgcgggggt
      841 cgccgactgg gctggcgacc tcatggtcgc gttcggcctc gccgggcgcg cggccgagat
      901 acggggcctg tacgctgacg cggcggcgtc ggataggtga tcgggcaggt gaacgcttcg
      961 ctcggcacgc ctccgccgtg cggtggatcg gggcggtggc catgcgcatg aacgctccca
     1021 tcctcttcct aggcatggcg acgctcgact tcgtgacggt cgtgccgcgc ctgcccgcgt
     1081 ccgacgaggt gttcgaggtc cgctccctga gcatgcacgg cggcggtccg gccgccacgg
     1141 cggccgtggc ctgcgcccgg ctgggtggcg acgcgcgctt cgccggcgcg ctcgggcggg
     1201 atccggttgc cgagacggtg ctcgcccagc tcgctagcga g
//
LOCUS       DAOQYE010000814         1238 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_146541_length_1238_cov_3.382079, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000814 DAOQYE010000000
VERSION     DAOQYE010000814.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1238
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146541_length_1238_cov_3.382079"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1111)
                     /locus_tag="PKN52_11375"
     CDS             complement(<1..1111)
                     /locus_tag="PKN52_11375"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNR00598.1"
                     /translation="MTRDPAQSFHGFLTQLPEYEGQVAAYHFFPPRKRVEGNGYDGPF
                     AELLERAGIVPYEHQAAALLAAAERRDVVVATATASGKSLVFQAPVLAGLEEGGTSLL
                     VYPTKALAGDQLLKLRGLAGAVGLTRAAERVQIYDGDTAAERRAGVRATAGALLTNPD
                     MLHYGILPHHAAWAPFLGRLRYLVLDELHAYRGVLGSHVANVVRRLLRVAALYGGRPT
                     VIAASATVGNPGQHAERLVGRPFEVVSADAAPRGAREFIIWRPPVAQGSERRRSANSE
                     AAALAAQFVRSDIKSIFFCNSRKGAELVRRYAAGLLGDELAERIESYRAGYTPEARAA
                     LEDAFRAGAVTVLTSTSALELGVDIASLNVVNMRNV"
BASE COUNT          180 a          450 c          439 g          169 t
ORIGIN      
        1 gcacgttgcg catgttcacg acgttcagcg aagcgatgtc gaccccgagt tccagtgcgc
       61 tcgtgctcgt cagcacggtg acggcgcccg cccggaaagc gtcctcgagg gccgcgcgcg
      121 cctcgggggt atagccggcg cggtagctct cgatgcgttc tgcgagctcg tcgcccagga
      181 gtccggcggc gtagcgccgc acgagctcgg cacccttgcg cgagttgcag aagaagatgc
      241 tcttgatgtc cgacctgacg aactgggcgg ccagcgcggc cgcctcggag ttggccgagc
      301 gtcgccgctc gctcccctgc gccacgggcg gacgccagat gatgaactcg cgcgcgcccc
      361 ggggggccgc gtcggccgac acgacctcga acggccgccc gactaggcgc tcggcgtgct
      421 gaccggggtt gccgacggtc gccgacgctg cgatcacggt cgggcgcccg ccgtagagcg
      481 ccgcgacgcg caagagccgg cgcaccacgt tcgcgacgtg ggagccgagc accccacggt
      541 aggcgtgcag ctcgtcgagg accaagtacc gcaaccggcc gaggaacggc gcccacgcgg
      601 cgtggtgcgg caggatgccg tagtggagca tgtcggggtt cgtcagaaga gcgccggccg
      661 tcgcgcgtac gcccgcgcgg cgctccgccg cagtgtcgcc gtcgtagatc tggacgcgct
      721 cggcggctcg ggtgaggccg acggcgcccg ccaggccgcg gagcttgagg agctggtcgc
      781 cggccagagc cttggtcgga tacacgagca ggctggtacc accctcctcc aggccggcca
      841 ggacaggcgc ctggaagacc agcgacttgc cggaggcggt ggccgtcgcc accaccacat
      901 cgcgacgctc cgctgccgcg agcagcgccg ccgcctggtg ctcgtacggg acgatcccgg
      961 cgcgctcgag cagttccgcg aacggtccgt cgtacccgtt cccctccacc cgcttgcgcg
     1021 gcgggaagaa gtgataggcc gccacctgcc cctcgtactc ggggagctgc gtgaggaaac
     1081 cgtggaagct ctgcgccggg tcgcgtgtca cctccaccga gactacccgc gcgcgccgtc
     1141 gtcccacgtc ggcccggtga gaccgatgct agactcgccc aaggccaacc gtcgccttgg
     1201 agtggtaacc cccgtgcagt ccgccttcct gtcaagcc
//
LOCUS       DAOQYE010000815         1235 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_147247_length_1235_cov_2.094915, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000815 DAOQYE010000000
VERSION     DAOQYE010000815.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_147247_length_1235_cov_2.094915"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="PKN52_11380"
     CDS             complement(<1..390)
                     /locus_tag="PKN52_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNR00599.1"
                     /translation="MRRIAVTLALAVVFGGLALAQGTIKIGVFLELSGRMAVTGNDTL
                     YGVNVAHEQQPTVNGMNVELSVCDNASTPEGAIACANRFVDEGVVAVIGSYSSSHSIP
                     AANVLQPAGIVMVSTGSTNPATTQIGDY"
     gene            complement(608..1012)
                     /locus_tag="PKN52_11385"
     CDS             complement(608..1012)
                     /locus_tag="PKN52_11385"
                     /inference="COORDINATES: protein motif:HMM:NF015581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HNR00600.1"
                     /translation="MTQEMGFKATFPGSLDDARDRVTGALAAEGFGVLTRIDVAATLQ
                     AKLGKAMEPYEILGACNPTLAHAALEVDRNVGLLLPCNVVLRGVDGAVEVSIVDPVAM
                     FSALPESAKARLEGAAADARGRLGRVAAALRG"
BASE COUNT          192 a          420 c          421 g          202 t
ORIGIN      
        1 gtagtcgccg atctgcgtgg tggcggggtt cgtggagccg gtggagacca tgacgatgcc
       61 ggccggctgg agcacgttgg cggccgggat cgagtggctg gaggagtacg agccgatcac
      121 ggcgacgacc ccttcgtcca cgaagcggtt ggcgcaggcg atggcgcctt cgggggtgga
      181 agcgttgtcg cagaccgaca gctccacgtt catgccgttg accgtcggct gctgctcatg
      241 ggccacgttc acgccgtaga gcgtgtcgtt gcccgtgacc gccatgcggc cggaaagctc
      301 taggaacacc ccgatcttga tggtgccctg ggcgagcgcg agcccaccga agacgactgc
      361 caacgcgagc gtaacggcta tgcgcctcat acttgaagtc ctctctcacg acctcggtcg
      421 tggtgcgtga ggcgcgtcgc ttggcctatc gcgccgtcgg cgccgccatc acgcgttact
      481 ttgtaagtcc ccgcactata cacagggtcg tgggagaggt gtcaacgctc gtcccacagc
      541 ggacccgagc ctcgtcatct gccgtcatgg acgcgagata ccgatccgga agccgaggtg
      601 acggggctta gccccgcagc gcggcggcca ctctgccgag gcgcccgcgc gcgtccgccg
      661 ccgctccctc gagccgggcc ttggcgctct ccggcagggc cgagaacatg gctaccgggt
      721 cgacgatgga gacctccacg gccccgtcaa caccccgcag caccacattg cacggcagga
      781 gaaggccgac gttcctgtcg acctcgaggg ccgcatgggc gagggtcggg ttgcacgcac
      841 cgaggatctc gtaaggctcc atcgccttgc cgagtttggc ctgcagcgtg gccgccacgt
      901 cgatgcgcgt gagcaccccg aagccctcgg cggcgagcgc gccggtgaca cggtccctgg
      961 cgtcgtcgag ggagcccggg aaggtggcct tgaaacccat ttcctgcgtc atcgctatcc
     1021 tcctggctgg aggggtctag ccgcggcccc gacgttcgac gcggcctcgt tgactcggat
     1081 cgcatggggc cagtgggcac cgctccgcct atgccgtcat cgtagccggc tcccggcacc
     1141 ggcggcgatc gccccattac gatggacaac gccaggatcg aagcgggtct tccgcgccac
     1201 agcgtccctg acgatgcgcg tctagcgcgc cgaga
//
LOCUS       DAOQYE010000816         1234 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_147452_length_1234_cov_2.326548, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000816 DAOQYE010000000
VERSION     DAOQYE010000816.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1234
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_147452_length_1234_cov_2.326548"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            265..561
                     /locus_tag="PKN52_11390"
     CDS             265..561
                     /locus_tag="PKN52_11390"
                     /inference="COORDINATES: protein motif:HMM:NF020473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HNR00601.1"
                     /translation="MGLVHALLSSAEAALGDDSSPLRLSGRDGVAGRKAARRSLDLLE
                     MLQVKTLGNLDAAERDALWSALRALRERLRAVGESELPVLDATASGLLTGDDPA"
     gene            558..1019
                     /gene="dtd"
                     /locus_tag="PKN52_11395"
     CDS             558..1019
                     /gene="dtd"
                     /locus_tag="PKN52_11395"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HNR00602.1"
                     /translation="MRALVQRVTRARVTVAERLVGGIDGGFLVLLGITHTDDAAVARR
                     LADKVRKLRVFEDEAGKMNLDIAAAGGDCLVISQFTLYGDTRGGNRPGFGAAARPEVA
                     EPLYREFVAALRETGVTVATGEFGAMMAVELVNSGPTTIWLDSEELFPAKA"
     gene            complement(1051..>1234)
                     /locus_tag="PKN52_11400"
     CDS             complement(1051..>1234)
                     /locus_tag="PKN52_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HNR00603.1"
                     /translation="DGSVEGITVAGKPVFGIQYHPEASPGPHDSSYLFTRFIEEVNSF
                     EGATALPAARMAVGAG"
BASE COUNT          197 a          433 c          450 g          154 t
ORIGIN      
        1 gcgtaacgcc agccgcgagc gcggagcgcc caaatggatc acctggaacg acatgaagga
       61 actcaagcgc gcgctggacg agctccctcc gaaagcgccc gtcttcaccc tcgacacggc
      121 tgagcaaggc gggttgttct gagctccccc gaccacgggc acggcagcgc ggactcgccg
      181 cgcgagcgcg gagcgccctc gcaggacgac gtcgcgcgcg caaagctcga cgcggaggcg
      241 agcgccgtcg cgagcaccag gttcatgggg ctggtgcacg ccctgctctc gtccgcggag
      301 gcggccctcg gcgacgacag cagcccgctg cgcctctccg gccgcgacgg cgtggcaggt
      361 cgcaaggccg cgaggcgcag cctcgacctc ctcgagatgc tgcaggtcaa gaccctcggc
      421 aacctcgacg ccgcggaacg cgacgcgctc tggtcagcgc tgcgagcgct gcgcgagcgc
      481 ctgagggcgg tcggggagag cgagctgccg gtgctcgacg cgacggcaag cggcctgctc
      541 accggcgacg acccggcgtg agggcgcttg tccagcgcgt gacgcgcgcc cgagtgacgg
      601 tggccgaacg gctcgtcggc gggatagacg gcggtttcct cgtcctgctc ggcatcaccc
      661 atactgacga cgccgccgtg gcgagacggc tggcggacaa ggtgaggaag ctgcgagtct
      721 tcgaggacga ggccggcaag atgaacctcg acatcgccgc cgccggcggt gactgcctcg
      781 tcataagtca gttcaccctc tacggggaca cgcgcggcgg caaccgcccg ggtttcggcg
      841 ccgcggcgcg acccgaggtg gccgaaccgc tctaccgcga gttcgtagcg gcgctgcgcg
      901 agaccggcgt aacggtggcg acgggggagt tcggggccat gatggcggtc gaactcgtga
      961 actcggggcc gacgacgatc tggctcgaca gcgaggagct cttccccgcc aaggcctgag
     1021 cacgcgcggc cgcgcgacca gacggctccc tcagccggcg ccgaccgcca tgcgagccgc
     1081 cggcagcgcc gtggcgccct cgaacgagtt cacttcctcg atgaagcgcg tgaacaggta
     1141 cgaggagtcg tgcggccccg ggctcgcctc ggggtggtac tggatgccga acaccggctt
     1201 gccggcgacg gtgatgccct cgaccgagcc gtcg
//
LOCUS       DAOQYE010000817         1231 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_148012_length_1231_cov_2.888605, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000817 DAOQYE010000000
VERSION     DAOQYE010000817.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1231
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_148012_length_1231_cov_2.888605"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /locus_tag="PKN52_11405"
     CDS             <1..529
                     /locus_tag="PKN52_11405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00604.1"
                     /translation="QAEVAPFRILRGMEVDILRDGTLDLSDEWLERLDIVLISVHSHF
                     DLSRAEQTARVVKAVSHPQVNVLAHPTGRVLGERDPFDLDLTAVFEACLANGVAVEHN
                     ASYQRLDLSDVNLMAARARGVTVTLGSDAHSVDQLATMRYGVDQLRRAWFTSADVLNT
                     RPLAEVRRFLGKRRG"
     gene            complement(713..>1231)
                     /locus_tag="PKN52_11410"
     CDS             complement(713..>1231)
                     /locus_tag="PKN52_11410"
                     /inference="COORDINATES: protein motif:HMM:NF024930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00605.1"
                     /translation="TGRAAEGACDVRSWRDIVAVSCGDWHSVGLRENGSAVAVGNNQR
                     GQCEVDLWRDLTAISAGCLHTAGLRYDGRVVAVGATMGACDVDRWEQVVAIGAGSYHT
                     VGVTVTGLVLAVGDNSYGQCDVDGWENIVEVAAGSTHTLGLCANGTVVSTGNSEDGRR
                     DTAHWPRLLPRN"
BASE COUNT          200 a          439 c          382 g          210 t
ORIGIN      
        1 ccaggccgag gtcgcgccgt tccgcatcct ccgcggcatg gaggtcgaca tcctgagaga
       61 cggcaccctc gacctctccg acgagtggct ggagcgcctc gacatcgtcc tgatcagcgt
      121 gcactcgcac ttcgacctga gccgggccga gcagacggcg cgggtcgtca aggccgtgag
      181 ccacccgcag gtcaacgtgc tcgcccaccc gacgggcagg gtgctcggcg aacgcgaccc
      241 cttcgacctc gacctcaccg ccgtcttcga ggcgtgcctc gccaacggcg tcgcagtcga
      301 gcacaacgcg tcctaccagc gcctcgacct gtcggacgtg aacctgatgg cggccagggc
      361 gcgcggcgtt accgtcacgc tcggctccga cgcccacagc gtcgaccagc tcgccaccat
      421 gcgctacggg gtcgaccagc tgcgccgcgc gtggttcacg agcgccgacg tcctgaacac
      481 gcggccgctg gcggaggtca ggcgcttcct ggggaagagg cgcggttagg gcgggcgtca
      541 caccctggta gccgtcaccc tggtggccgt cacttaggta gttgaagtac tgctacgtag
      601 ccgaggtact gcaaacgcgc tcacctcgtg cgcgtccgcg cgggtgtcag cctatcgctg
      661 acttgggacg cgcggccaag catccacgga tgccgatgcc gatgagcctt ggctagtttc
      721 gggggaggag tctcggccag tgcgcagtat cacgcctgcc gtcctcgctg tttccggtgc
      781 tgacaacggt gccgttagcg cacagaccga gcgtatgagt cgagcctgct gccacctcga
      841 cgatgttctc ccagccgtca acgtcgcact gtccatagct gttgtctcca acggcgagga
      901 ccagcccggt cacggtgacg cccacggtgt gatagctccc cgcacctatc gcgactacct
      961 gctcccatct gtcaacgtcg cacgcaccca tggtcgctcc tacagcgacg acccgtccgt
     1021 cgtacctgag cccagcggta tgcaggcatc cggcggagat ggcagtgagg tctcgccaga
     1081 ggtcgacttc gcactgtcct cgctggttgt tgccgactgc taccgctgat ccgttctcgc
     1141 gaagacctac cgaatgccag tcgccgcagg agactgccac gatgtctcgc catgacctca
     1201 catcgcaagc gccctcggca gcgcggccag t
//
LOCUS       DAOQYE010000818         1231 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_148028_length_1231_cov_2.634354, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000818 DAOQYE010000000
VERSION     DAOQYE010000818.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1231
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_148028_length_1231_cov_2.634354"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..924)
                     /locus_tag="PKN52_11415"
     CDS             complement(<1..924)
                     /locus_tag="PKN52_11415"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HNR00606.1"
                     /translation="MTRPLALVLMLLLGATVLSLNLGSFRITPGELLRTAAGRGDANQ
                     ELVLYRIRLPRLTMAVLVGIGLATAGAVLQGVTRNSLADPGILGLTAGAGLAVILLIY
                     LQERGLAVPPGIRPVVAFAGAGLAAAVTFLAARRRGVVLPSQLLLAGIAVNAGLSAVT
                     LVLSMRLDRKLYDQAVTWLAGTLVGRDWQQVLSLLPWLIVLLPILWARSRPLDILGLG
                     DGAATGLGVDVRGERAWLLLAAVGLAGSSVALAGGIGFVGLLGPHLARRLVGPQHGRM
                     LPLAACLGALLVVSADIVARNLLRPVELPVGI"
     gene            complement(924..>1231)
                     /locus_tag="PKN52_11420"
     CDS             complement(924..>1231)
                     /locus_tag="PKN52_11420"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HNR00607.1"
                     /translation="TGRLRFLASVCTLVLAGGAVALAGGVGFVGLVVPHMMRFLVGVD
                     YRWIIPGSAVFGALLLVLADLGARMVNPPYELPVGLITALIGVPFFIYLARRGDQGL"
BASE COUNT          220 a          425 c          459 g          127 t
ORIGIN      
        1 gatcccgacg gggagctcga ctggtcgcag caggttacgt gccacgatgt cggcgctcac
       61 caccaggagc gccccgaggc acgctgccag ggggagcatg cgcccatgct gcggtccgac
      121 gagccgccgc gccaggtgcg gcccgagcag gccgacgaac ccgatgccgc cggccaaggc
      181 cacgctgctc ccggccagcc ccacggccgc gagcagcagc caggcgcgtt cgccgcgcac
      241 gtcgaccccg aggccggtgg cggcgccgtc accgagcccg aggatgtcga gcggtcgcga
      301 gcgcgcccac agtatgggaa gcaagacgat cagccagggc agtagggaga ggacctgttg
      361 ccagtcgcgc cccaccagcg tccccgcgag ccacgtcacg gcctgatcgt agagcttgcg
      421 gtctaggcgc atggagagca cgagcgttac ggcggacagc ccggcgttca ccgcgatccc
      481 cgccagcagg agctgcgagg gcagcaccac gccgcgccgt ctggccgcga ggaaggtgac
      541 ggcggccgcg agcccggccc ccgcgaaggc gacgaccggt ctgatgccgg gcggaacggc
      601 gagaccgcgt tcctgcaggt agatgagcag gatgacggcg agaccggctc cggccgtcag
      661 tccgaggatg ccagggtcgg cgagcgagtt gcgcgtgaca ccttgcagga ctgcgcccgc
      721 cgtcgcgagc ccgatcccga cgaggacggc catcgtgagg cgtggcaggc ggatccggta
      781 gagcacgagc tcttggttcg catcgccgcg ccccgcggcg gtccgcagga gctcgccggg
      841 cgtgatgcgg aacgacccaa ggttgaggct cagcacggtg gcgccgagca gcaacatgag
      901 gaccagggct aacgggcggg tcatcacaac ccctggtcgc cgcggcgggc taggtagatg
      961 aagaacggca cgccgatcag cgcggtgatg aggccgactg ggagctcgta gggcgggttg
     1021 accatcctgg cgccgaggtc ggccagcacg agtagcaagg ccccgaatac ggcggagccc
     1081 gggatgatcc accggtagtc gacgccgacc aagaaccgca tcatgtgcgg cacgaccagc
     1141 ccgacgaagc cgacgccgcc ggcgagcgcc acggcgccgc ccgcgaggac gagcgtgcag
     1201 acgctcgcga ggaagcgcag ccggccggta c
//
LOCUS       DAOQYE010000819         1229 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_148490_length_1229_cov_2.134583, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000819 DAOQYE010000000
VERSION     DAOQYE010000819.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1229
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_148490_length_1229_cov_2.134583"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1229
                     /gene="gpmI"
                     /locus_tag="PKN52_11425"
     CDS             <1..1229
                     /gene="gpmI"
                     /locus_tag="PKN52_11425"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HNR00608.1"
                     /translation="GLVSDGGVHSDLGHLLALLELAGRERVERVRVHAFTDGRDSAPD
                     GGARYLAQVETALARARDAGSDARVATVCGRYYAMDRDRRWERTKLAYDLVVCGAAEH
                     VAPSAVAAVRAAYARGETDEFVRPTVVVADGVPADDGTVRDGDAVLFFNFRTDRARQL
                     SHAFVGGEGWNEFERCRAPRVTFASLMEYDKELRVPFAFAVPPVMETLPEVLARAGMK
                     QYHTAETEKYAHVTYFFDAQREAPQVGEERRMVQSPKVATYDLQPEMSARELAALTAA
                     RLRERDDDFVLVNFANPDMVGHTGVLAAAVTACEVVDSCLAEVLEAVLARGGAAIVVA
                     DHGNAERMLEADGSPYTAHTTNPVPCVLVSDDAALAGARLRSGGILGDVAPTVLDLLG
                     VPQPAAMTGRSLLVRG"
BASE COUNT          173 a          421 c          466 g          169 t
ORIGIN      
        1 tgggcctggt gagcgacggc ggcgtgcact ccgacctcgg gcacctcctc gcgctgctag
       61 agctcgccgg tcgcgagcgc gtcgaacggg tgcgcgtcca cgccttcacg gacggccgcg
      121 actcggcgcc cgacgggggg gctcgctacc tcgcgcaagt cgagaccgcg ctggccaggg
      181 cgcgtgacgc aggcagcgac gcgcgcgtgg cgacggtctg cggccggtat tacgccatgg
      241 accgcgatcg gcggtgggag cgcaccaagc tcgcctacga cctggtggtg tgcggggcgg
      301 cggagcacgt ggcaccgagc gccgtcgccg cggtccgggc cgcttacgcg cgcggcgaga
      361 ccgacgagtt cgtcaggccg acggtcgtcg tggcggacgg cgtaccggct gacgacggca
      421 cggtgaggga tggcgacgcc gtcctcttct tcaacttcag aaccgatcgg gcgcggcagc
      481 tcagccatgc cttcgtcggc ggcgagggtt ggaacgagtt cgaacggtgc cgggcgccgc
      541 gcgtgacctt cgcctcactg atggagtacg acaaggagct gcgcgtcccg ttcgccttcg
      601 cggtcccgcc cgtcatggag acgctgcccg aggtcctggc ccgcgcaggc atgaagcagt
      661 accacacggc cgagacggag aagtacgcgc acgtgacgta cttcttcgac gcgcagcgcg
      721 aggcgccgca agtgggcgag gagcgacgca tggtccagtc ccccaaggtg gcgacctacg
      781 acctgcaacc cgagatgagc gcgcgggagc tggcggccct gaccgcggcg cggctgcgcg
      841 agcgcgacga tgacttcgtc ctcgtcaact tcgccaaccc cgacatggtg ggccacacgg
      901 gcgtgttggc cgcggccgtc acggcttgcg aggtcgtcga ctcatgcctc gcggaggtgc
      961 tcgaggcggt gctggcgcgg ggcggggccg ccatcgtggt cgccgaccac ggcaacgccg
     1021 agaggatgct cgaggcggac ggcagcccgt acaccgccca cacgacgaac cctgtgccgt
     1081 gcgtgctcgt gagcgacgac gcggccctgg cgggcgcgcg cctgcgctcc ggcggcatcc
     1141 tcggcgacgt agctccgacg gtgcttgacc tgctcggcgt gccgcagccg gcggcgatga
     1201 cgggcagatc gcttctggtg cggggctga
//
LOCUS       DAOQYE010000820         1228 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_148651_length_1228_cov_2.276215, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000820 DAOQYE010000000
VERSION     DAOQYE010000820.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1228
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_148651_length_1228_cov_2.276215"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="PKN52_11430"
     CDS             <1..327
                     /locus_tag="PKN52_11430"
                     /inference="COORDINATES: protein motif:HMM:NF019522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HNR00609.1"
                     /translation="HVNPAFGLAELYRHGMNVGLDHIGSLVNTLVLAYTGSALPLLVL
                     LFINDFSISRALNLEMVAAEVVHTLVGSIGLVLAVPFTTLIAAAMFRGARLPLIAGEL
                     EHGHHH"
     gene            327..>1228
                     /locus_tag="PKN52_11435"
     CDS             327..>1228
                     /locus_tag="PKN52_11435"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HNR00610.1"
                     /translation="MVRVDRSLLIAARVALGVLLLFALAGQAIAQERYNFLAVDDEIV
                     DAAGPYYFIVQGDSVNAFARLEPFAEAIRVDAKVDQTRGTVVLSGATHRLELRLHESV
                     AEGLEFSSLNLAVDGTPFARAVPGGIIVDGKVYVPITPVVEAFGGRVDWHPDTHIIAV
                     GTLEQAPDNRPHYGPPRVGLHDDFTRVALTLPPSQAVTVGVSQGTLTIRVPGAELEEF
                     DQRFDAGPLTRLYSDAASGSAALMLTVKHEVSADGAGFRVGRTDDAFYVDVGPGLVSS
                     PGDEHLITDQPAPVPAPTPDFEVQR"
BASE COUNT          197 a          391 c          387 g          253 t
ORIGIN      
        1 cacgtcaacc cggccttcgg cctcgcggaa ctatatcggc atggcatgaa cgtcggcctg
       61 gaccacatcg gttcccttgt caacacgctt gttctcgcct acaccggctc tgcgctcccg
      121 ctcctggtgc tcctcttcat caatgacttc agcatcagtc gcgccctgaa cctagagatg
      181 gtcgcggcgg aggtagtgca cacgctcgta ggttcgatcg ggttggtgct ggctgtaccg
      241 ttcaccaccc tgatcgctgc agcgatgttt cgcggcgcgc ggctgccttt gatagctggc
      301 gaactcgagc acggtcatca ccactgatgg ttcgtgtcga ccgatcactc ctgatcgccg
      361 cccgagtcgc gttgggtgtc ctgctgctct tcgctcttgc cggccaggcc attgcccagg
      421 agcgctacaa cttcctggcg gtcgatgatg agatcgttga tgcggcaggt ccttactact
      481 tcatcgttca aggagattcg gtcaacgcct tcgcgaggct ggagccgttc gccgaggcga
      541 tcagggtaga tgcgaaggtg gaccagacgc gtgggaccgt ggtcctctcg ggtgctaccc
      601 accgactgga gctcaggctt catgagagtg ttgccgaggg gctggagttc tcctccctca
      661 acctggctgt ggacgggaca cccttcgccc gtgccgtgcc cggcggcata atcgtcgacg
      721 gcaaagtgta cgttccgatc acgccggtcg tcgaagcctt cggcgggagg gttgattggc
      781 atcctgacac gcatatcatc gcggtcggca cgcttgagca ggcgccggac aaccggccgc
      841 attacggtcc tccaagggtc ggcctccacg acgacttcac cagggtcgcg ttgacgctcc
      901 caccctcgca ggcggtgaca gtgggcgtct ctcagggcac cttgacgatc cgggtccctg
      961 gggcggagtt ggaggagttc gatcagcggt tcgacgcagg cccgcttacg cgcttgtact
     1021 cggacgcggc atcgggatcg gctgcactga tgctgacggt gaagcacgag gtatcggccg
     1081 atggtgcggg cttccgagtg ggaagaacgg atgacgcctt ctacgtggac gtgggccctg
     1141 gcctcgtcag ctcacccggc gacgagcacc tgatcactga ccagcccgca cctgtgcccg
     1201 cgcctacgcc cgatttcgag gtgcaacg
//
LOCUS       DAOQYE010000821         1227 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_148788_length_1227_cov_3.326792, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000821 DAOQYE010000000
VERSION     DAOQYE010000821.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1227
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_148788_length_1227_cov_3.326792"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1227
                     /locus_tag="PKN52_11440"
     CDS             <1..>1227
                     /locus_tag="PKN52_11440"
                     /inference="COORDINATES: protein motif:HMM:NF015592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00611.1"
                     /translation="GGATGSGVGADASALREAGADIVVPDLSPLADRVAGGGVDSERW
                     PADEWQLVEKRPPAGVDGASETLFALGNGYLGLRGNPEEGGPAHEHGTFINGFFEAWE
                     IEYPETAYGFATAGQTIVNVPDAKTIRLSADGERLDLAAAEVEEYERSLDLRSGVLRR
                     RLVWRTSTGKRLGIDSARLVSFKRRNLALVTYRVTALDAPVRVELESLLINRQDGEGE
                     FAARRRVDGLDPRRSDELEGRVLEPTGRTVSEQRVGLAFQTSRSRLGLAALVEHEPTG
                     QTVVHPDWCATRFAFDLQAGESAAIVKKVVYVDGGQVGSNDGQELLATAGKLMDDLPG
                     TEAVIEEQRGWCGGFWERADIRVHAEVGEGRRIAEEETAPQEAGERALQQAVRWNVFQ
                     LAQAAARADGRGVSAKG"
BASE COUNT          203 a          401 c          469 g          154 t
ORIGIN      
        1 gggtggtgcg acgggaagcg gggtcggcgc cgacgcctcc gcgctccgcg aggcgggagc
       61 cgacatcgtc gtgcccgacc tctctccgct ggcggaccgc gtggctggcg gaggcgtcga
      121 cagcgagcgc tggccggcag acgaatggca actcgtcgag aagcggccgc ccgccggagt
      181 cgacggcgct agcgagacgc tgttcgctct cggcaacggc tacctcggcc tgcgggggaa
      241 ccccgaggag ggcgggccgg cgcatgagca cggaaccttc atcaacggct tcttcgaggc
      301 ctgggagatc gagtaccccg agacggcgta cgggttcgcc acggccggtc aaaccatcgt
      361 caacgtgccg gacgccaaga cgatccgcct cagcgccgac ggcgagcgcc tggacttggc
      421 ggcggccgag gtggaggagt acgagcgcag cctcgacctg cgctccggtg tgctgcggcg
      481 gcggctcgta tggcgaacct cgactggcaa gcggctgggg atcgactccg cccggctcgt
      541 gtcgttcaag cgccgcaacc tggcgctcgt cacataccga gtcacggcgc tggacgcgcc
      601 agtgcgcgtt gagctggagt cactgctcat caaccggcag gacggcgagg gcgagttcgc
      661 cgcgcgccgg cgggtcgacg gcctcgaccc acgcaggagc gacgagctcg agggtcgggt
      721 gctcgagccg acgggccgaa cggtgagcga gcagcgagtc ggactagcct tccagacgag
      781 ccggtcccgc ctggggttgg cggcgctcgt ggagcacgag cccacaggcc agacggtcgt
      841 acatccggac tggtgcgcga cgcgcttcgc gttcgacctc caagccggcg agagcgccgc
      901 catcgtgaag aaggtcgtct acgtggacgg cgggcaggtc gggtccaacg acggccaaga
      961 actgctcgcc accgccggca agctcatgga cgacctgccg ggcacggaag ccgtgatcga
     1021 ggagcagcgg ggctggtgcg gtggtttctg ggagcgcgcc gacatccgcg tacatgcgga
     1081 ggtaggagag ggccgtcgca tagcggagga ggagaccgca ccgcaagagg ccggagagcg
     1141 cgcccttcag caagccgtcc gctggaacgt cttccaactc gcccaggccg cggcgcgagc
     1201 cgatgggcgc ggcgtgagcg cgaaagg
//
LOCUS       DAOQYE010000822         1225 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_149229_length_1225_cov_3.880342, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000822 DAOQYE010000000
VERSION     DAOQYE010000822.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1225
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_149229_length_1225_cov_3.880342"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /locus_tag="PKN52_11445"
     CDS             complement(<1..521)
                     /locus_tag="PKN52_11445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNR00612.1"
                     /translation="MIARGAGRPAPSRGRFKLSAPARAGLLFILPALVYIVLTQLVPV
                     LYALYVSFTDYSPLNRGAPRWVGLQNYKAMIFSREFQNALWVTVRFSAAVIALNVAIS
                     LGLALLLEGASRVLRWLQVVLFLPMVTSVAAVSIVWLLLYDRDFGLLNQIVNALGLPS
                     VGWLNTVAWALPSL"
     gene            complement(518..>1225)
                     /locus_tag="PKN52_11450"
     CDS             complement(518..>1225)
                     /locus_tag="PKN52_11450"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNR00613.1"
                     /translation="QRTGLLIGSNGTAIQQPFSSFFWQAGGEWFSPDGREVTFDGPAA
                     VKAIEFMQQLFDPATGVTRLGENPGASPVNPLVTGQAAMMFGSMEMTSGLRTTNPDAF
                     ELLAVAPPTKDVDQATFYSFAGFFISQDSSHKDEAWQYISFALSPEWLEAFNSAAGSL
                     PPRSSLADSAFLAESPALKAYSDNLQFAHGNPNVAIWVQARDITAAHLESAVYGRETA
                     EAAAKAIQQEIEALLNP"
BASE COUNT          218 a          364 c          452 g          191 t
ORIGIN      
        1 aggctcggga gcgcccaggc cacggtgttc aaccatccga cgctgggcag ccccagggcg
       61 ttcacgatct ggttcaggag tccgaagtcc cggtcgtaga ggagcagcca gacgatgctg
      121 acggcggcga cgctcgtgac catgggcagg aagaggacta cctggagcca tcgcaggacg
      181 cgggaggcgc cctcgagcaa gagcgcgagc ccgagcgaga tggcgacgtt caacgcgatg
      241 acggccgcgg agaaccgcac ggtgacccag agggcgttct ggaactcgcg cgagaagatc
      301 atcgccttgt agttctggag gccgacccat ctcggcgcac ccctgttcaa cggggagtag
      361 tcggtgaagg aaacgtacag ggcgtacaag acgggcacca gttgggtgag gacgatgtag
      421 acgagcgctg gaaggatgaa gagcaggccg gcccgggcgg gcgccgagag cttgaacctg
      481 ccgcggcttg gtgctggccg gcctgctccc ctcgcgatca tgggttcagc agcgcctcga
      541 tctcttgctg gatcgccttg gcggcggctt cggccgtctc acgcccgtag acggccgact
      601 cgaggtgcgc agccgtgatg tcgcgggctt gcacccagat ggccacgttc gggttgccgt
      661 gcgcgaactg gaggttgtca gagtacgcct tcagggcagg cgactcggcg aggaaggcgg
      721 agtcggccag actggagcgc ggaggcaggg agccggcggc gctgttgaac gcctccaacc
      781 actcggggct gagggcgaag cttatgtact gccaggcctc gtccttgtgg gagctgtcct
      841 gcgagatgaa gaagccggcg aacgagtaga aggtggcctg gtcgacatcc ttcgtcggcg
      901 gcgccacggc gagcagctcg aaggcgtccg ggttggtggt ccttagcccg gaagtcatct
      961 ccatcgagcc gaacatcatc gccgcctgcc cggtgaccag gggattgacg gggctagcgc
     1021 cggggttctc tcccagccgc gtcacaccgg tggccgggtc gaagagctgc tgcatgaact
     1081 cgatggcctt gacggcggcg gggccgtcga acgtcacttc cctgccgtcc gggctgaacc
     1141 attcgccacc ggcttgccag aagaaggagg agaacggctg ctggatggcc gtgccgttgg
     1201 agccgatcag gaggccggtg cgctg
//
LOCUS       DAOQYE010000823         1223 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_149680_length_1223_cov_2.576199, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000823 DAOQYE010000000
VERSION     DAOQYE010000823.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1223
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_149680_length_1223_cov_2.576199"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="PKN52_11455"
     CDS             <1..468
                     /locus_tag="PKN52_11455"
                     /inference="COORDINATES: protein motif:HMM:NF015962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0050532 - 2-phosphosulfolactate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HNR00614.1"
                     /translation="VAGLDVVMVSENAPRWLEQLGDARHVMLGSLYNAEAVARRAAAV
                     ATERIDLVCSGFAGEPDLDDTLAAALIAGLLKRRLAGAQVRGATRFATALLRAFPEPV
                     DVLWRSLAGHYLRSIGLEQDIGFAARVSVSDSVPELRSVALGEHGPLYRFETA"
     gene            465..>1223
                     /locus_tag="PKN52_11460"
     CDS             465..>1223
                     /locus_tag="PKN52_11460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043163 - cell envelope organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HNR00615.1"
                     /translation="MSERGAGARSVETASEAAVMDPSARPDPHADAAERAAGRPAVMP
                     ERVDVLGFPVDVVDLQDAVRWVLDAVADGGKPRLVVTLNPEIVVQGLGDPDLAAALRS
                     ADLSVADGVGIALAARRAGHPLPGRVPGIDLVTELFHVGGPNVRAYFLGAKPGVAERA
                     AAAAARRFGVTVAGHHHGYFDRVGESARICEGVRAARPDVLLAGLGEGQELFLDRHAQ
                     ALGAKVLIGVGGTLDVLAGEVRRMPAWTTRLGVEW"
BASE COUNT          149 a          447 c          451 g          176 t
ORIGIN      
        1 gtagccggcc tcgacgtcgt catggtgtcc gagaacgccc cacgctggtt ggagcagttg
       61 ggtgacgcgc ggcacgtcat gctcggctcc ctctacaacg ccgaggcggt cgcccgacgc
      121 gccgccgcgg tggctacgga gcgcatcgac ctcgtctgct ccggcttcgc gggcgagccc
      181 gacttggacg acacgctggc cgccgccttg atagccggcc tcctgaaacg acgcctcgct
      241 ggggcgcagg tgcgcggcgc aacgcgtttc gcgacggcgc tcctgcgggc gttcccggaa
      301 cccgtcgacg tgctctggcg ctcgctggcc ggccactacc ttcgctccat cgggctcgag
      361 caggacatcg gcttcgcggc gcgcgtgtcg gtgagcgaca gcgtgccgga gctgcgctcc
      421 gtcgctcttg gcgagcatgg gcccctgtac cgcttcgaga cagcttgagt gaacgaggtg
      481 ccggggccag gagcgtcgag accgcttccg aagcggccgt gatggacccc tccgcgcgcc
      541 cggatccgca cgccgacgcc gccgaacgcg ccgccggccg gccggccgtc atgccggaac
      601 gggtcgacgt actgggcttc cccgtcgacg tcgtggacct gcaagacgcg gtgcgctggg
      661 tgctggacgc cgtcgcggac ggcggcaagc cccgcctcgt cgtcaccctc aacccggaga
      721 tcgtcgtgca aggcctcggc gatccggacc tcgccgcggc cctcagaagc gctgacctga
      781 gcgtcgccga cggggtcggc atcgcgctgg cggcgcgacg cgcgggccac cccctgcccg
      841 gcagggtgcc ggggatcgac ctcgtgacgg agctgttcca cgtcggcggg ccgaacgtgc
      901 gcgcctactt cctcggcgcc aagcccggcg tggcggagcg cgctgccgct gccgcagccc
      961 ggcgcttcgg cgtgaccgtg gccggtcacc accatggcta cttcgaccgc gtcggcgagt
     1021 cggcccggat ctgcgagggc gtgagggcgg cgcgccccga cgtgctactg gcggggcttg
     1081 gcgagggcca ggagctgttc ctcgaccgcc acgcccaagc gctcggcgcc aaggtcctca
     1141 tcggagtcgg cggcaccctt gacgtcctgg cgggcgaggt gaggcgcatg ccggcctgga
     1201 cgacgcggct cggcgtggag tgg
//
LOCUS       DAOQYE010000824         1220 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_150254_length_1220_cov_4.021459, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000824 DAOQYE010000000
VERSION     DAOQYE010000824.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1220
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150254_length_1220_cov_4.021459"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1052
                     /locus_tag="PKN52_11465"
     CDS             <1..1052
                     /locus_tag="PKN52_11465"
                     /inference="COORDINATES: protein motif:HMM:NF014010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HNR00616.1"
                     /translation="MLSSSNAATAVALGLHVAGAVSLETALVLVVGGNAGAGVLPLMV
                     SRSFDSNAQRAGLMQMLVLVFGAMAIVLFREPATHLLQSIGGGGAREVANAHTFFNLA
                     VAVFGTLFCRLLAPLSAKLVPMTDDDSAPKYLRADAVSDPQLGLALAKRETVRISDQV
                     VVMTERAMEYLSTGRWDPGPIAARELKVDRLTFRVVDYVARIRRANGEDPISERLLLT
                     ATELEHMGDQIRRLARREEKLKSEGVEFSKEGRSELTETGERVLQRMRNAFTALATGD
                     KLMARSVIAGRHALEEHIGSMRVAHLARLQAQLPESRASSSHHLEVLTLLRDLDASVT
                     RVAGWAEEMEEHGGR"
BASE COUNT          176 a          449 c          408 g          187 t
ORIGIN      
        1 ccatgctctc cagttccaac gcggccacgg ccgtggccct cggcttgcac gtcgcggggg
       61 cggtctcgct cgagacggcg ctcgtcctcg tggtcggggg caacgcgggc gccggcgtgc
      121 tgcccctgat ggtctcgcgc agcttcgact cgaacgccca gcgcgctggc ctcatgcaga
      181 tgctcgtcct ggtgttcggc gcgatggcta ttgttctctt ccgcgagccc gccacgcacc
      241 tcctgcagtc gatcggcggc ggcggcgccc gcgaggtcgc caacgcccac accttcttca
      301 acctggcggt ggccgtcttc ggcacgctct tctgccgcct gctcgccccc ttgagcgcca
      361 agctcgtgcc catgacggac gacgactccg cgcccaagta cttgcgcgcg gacgccgtct
      421 ccgaccccca gctcgggctc gctctcgcca agcgcgagac ggtccgcatc agcgatcagg
      481 tggtcgtcat gacagagcgg gccatggagt acctgtccac cggcagatgg gatcctggcc
      541 ccatcgccgc cagggagctg aaggtcgacc gcctcacctt cagggtcgtc gactacgttg
      601 cgcgcatccg tagggccaac ggcgaggacc ccatatccga gcgcctgctc ctcaccgcca
      661 cggagctcga gcacatgggc gatcagatca ggcgcctggc ccggcgcgag gagaagctga
      721 agagcgaggg cgtcgagttc agcaaggagg ggcgctccga gctgaccgag acgggcgagc
      781 gcgtcctcca gcgcatgcgc aacgcgttca cggccctcgc gacgggcgac aagctgatgg
      841 cgcgcagcgt gatcgcgggt cggcacgctc tggaggagca catcggcagc atgcgcgtcg
      901 cgcacctggc caggttgcag gcgcagctcc ccgagtcgcg cgcgtccagc tcgcaccacc
      961 tcgaggtcct cacgctcctg cgcgacctcg acgccagtgt cacgcgcgtc gccggctggg
     1021 cggaggagat ggaggagcat ggtggacgct gacggagcac gcatgccacg cgatctctcg
     1081 tcctcggggg tgccgacgcg tcccccgcac gccttctcgc tcgacctggg cgacgggctc
     1141 gccctcaggc tgcgtgagcc gcaccacgtc gccgagtacc gggttctgct cgaggccaac
     1201 ctcgagcacc tccagcggtg
//
LOCUS       DAOQYE010000825         1220 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_150378_length_1220_cov_2.012017, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000825 DAOQYE010000000
VERSION     DAOQYE010000825.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1220
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150378_length_1220_cov_2.012017"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1075
                     /locus_tag="PKN52_11470"
     CDS             <1..1075
                     /locus_tag="PKN52_11470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00617.1"
                     /translation="CLSLFGPALAASGAVGFTLGIANEVVVGAWNPLRVETRDLPRST
                     LTVMLDVGTLRDGEVPIELTFELSGGGGVNAFETELFVPPFRSLAWRVTTADRVVGSG
                     SLAGRETDARPLDLVLAARAPAGHAAISRAVGGAARLVEVTLSDLPASPAAYDGVRSL
                     TIEGSAPVDAVVAAAVAGASVVLAGGRGSLAGPLAALVPEAGATALGAGRVTLVDAPL
                     VEAAVAAIGGVGTLGRAGQLLAAVLEEPLVSAPRQAPVATMLIGSVVYALLALVAVRL
                     GGAPGLLASLIVAVFCGFGAWQALRPAPPPYIGSVALAQVAARWPSSTRLSRASPYPA
                     QCSSSRPVRRRCVPSPTSAARRR"
BASE COUNT          123 a          429 c          475 g          193 t
ORIGIN      
        1 ttgcctgagc ctgttcgggc cggcgctcgc ggcgagtggg gcggtgggtt tcacgctcgg
       61 catcgccaac gaggtggtcg tcggcgcttg gaacccgctg cgcgtggaga cccgcgacct
      121 gcccaggagc acgctgacgg tcatgctgga tgtcggcacg ctacgagacg gcgaggtgcc
      181 gatcgagctg accttcgagc tgtcgggagg cggcggagtc aacgctttcg agacggaact
      241 gttcgtaccc cccttccgca gcctcgcttg gcgggtgacg acagccgacc gggtggtggg
      301 cagcgggagc ctcgcgggca gggagacgga cgcccgcccg ctggatctcg tcctcgccgc
      361 gcgcgcgcct gccggccatg cggcgatctc gagggccgtg ggaggagccg cgcggctcgt
      421 cgaggtcacg ctctccgact tgcctgcctc gccggccgct tacgacgggg tgaggagcct
      481 gacgatcgaa ggctcggcgc cggtggacgc cgtggtggcc gccgccgttg cgggcgcctc
      541 cgtcgtgttg gccggtggcc gcggctcgct ggcggggccg ctggccgcgc tcgtgcccga
      601 ggccggggcg acggcgctcg gcgccggcag ggtgacgttg gtggacgcgc cgttggtgga
      661 ggccgccgtg gcggccatag gcggggtggg caccctcggt cgagccggcc aactgctggc
      721 cgccgtgttg gaggagccgt tggtcagcgc gccaaggcag gccccggtcg ccaccatgct
      781 catcggctcg gtcgtctacg ccctgttggc gctcgttgcc gtcaggctcg gtggggctcc
      841 cggtttgctg gcctcgctga tcgtggccgt cttctgcggc ttcggcgcct ggcaggcgct
      901 gcgcccggcc ccgcccccgt acatcggctc cgtcgcgctg gcgcaggtgg cggcccgctg
      961 gccgtcatcc acgaggctat cgagagcctc accctaccca gcgcagtgct cgagttcccg
     1021 gccggtgcgg cgccggtgcg tccccagccc tacttcggca gcgcggaggc ggtgagcgtc
     1081 cccgcggccg cctggcgctc cgtcacggtg cgcctgccgc cgcgagcggt cggcgccccg
     1141 ttctcggtcg cgggcaactc ggtccgcaac gaagcgagcg cgccgcttac ggatgtctac
     1201 gtgacggggc tgggcgcgca
//
LOCUS       DAOQYE010000826         1220 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_150383_length_1220_cov_1.987124, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000826 DAOQYE010000000
VERSION     DAOQYE010000826.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1220
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150383_length_1220_cov_1.987124"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..923)
                     /gene="nusA"
                     /locus_tag="PKN52_11475"
     CDS             complement(<1..923)
                     /gene="nusA"
                     /locus_tag="PKN52_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HNR00618.1"
                     /translation="MNKEFVDALNLLAAERNLDKEQLLADLEDALGQAFERNVMPGHQ
                     IDVELDPESGEMDIMVIRRVVETVEDPTTEISLADALDLDEEVEVGMEMEFPVDPEKF
                     TRIAVQTTKQVITQKIREAERAYVFEEYKDRAGDIMTGVVARTDNKRNVFVDLGRGEA
                     IMPAKEQIQGERYMVGMRLKVYVQNVELSPRGPSIHVSRAAPELLEYLMRQEIPEVAD
                     GTVELKAVAREAGQRSKVAVSSNNPNVDPIGACIGHRGSRIQAVTAELQRERVDIIQW
                     DPNVREFIRNALSPAKVGNIELDTEAKAAKVT"
     gene            complement(1008..>1220)
                     /locus_tag="PKN52_11480"
     CDS             complement(1008..>1220)
                     /locus_tag="PKN52_11480"
                     /inference="COORDINATES: protein motif:HMM:NF036577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HNR00619.1"
                     /translation="GAERPLRRARHFERFHDLLVKFRAGGETFKGKVRAVTEDAVTVE
                     VDGAQRVVRIADIVSARLAEWPESPR"
BASE COUNT          154 a          417 c          410 g          239 t
ORIGIN      
        1 gtcaccttgg cggccttggc ctccgtgtcg agctcgatgt tgccgacctt ggcgggcgag
       61 agagcgttgc ggatgaactc gcgaacgttc gggtcccact ggatgatgtc gacgcgctcg
      121 cgctgcagct cggccgtcac ggcctggatg cggctgccgc ggtggccgat gcaggcgccg
      181 atcgggtcga cgttggggtt gttcgagctc accgccacct tggagcgctg gccggcctcg
      241 cgggcgacgg ccttgagctc caccgtgccg tcggccacct cggggatctc ctggcgcatg
      301 aggtactcga ggagctcggg cgcggcgcgc gacacgtgga tcgacggccc gcgcgggctg
      361 agctccacgt tctgcacgta gaccttgagg cgcatgccga ccatgtagcg ctcgccctgg
      421 atctgctcct tggcgggcat gatggcctcg ccgcggccca ggtcgacgaa gacgttgcgc
      481 ttgttgtcgg tgcgtgcgac gacgcccgtc atgatgtcgc cggcgcggtc cttgtactcc
      541 tcgaagacgt aggcgcgctc ggcctcgcgg atcttctgcg tgatgacctg cttggtcgtc
      601 tgcacggcga tgcgcgtgaa cttctcgggg tcgaccggga actccatctc cattccgacc
      661 tcgacctctt cgtccaggtc gagcgcgtcg gcgagcgaga tctcggtggt ggggtcctcg
      721 accgtctcga cgacccgacg gatgaccatg atgtccatct cgcccgactc ggggtcgagc
      781 tcgacgtcga tctggtggcc cggcatgacg ttgcgctcga acgcctggcc cagggcgtcc
      841 tcgaggtccg ctagcagctg ctccttgtcg aggttgcgct cggctgccag caggttgagc
      901 gcgtctacga actccttgtt catctagtcc aactccctac ctattcggtt gtggccactg
      961 ccgcagtccg cgggtggcgg tgtgctcacg ccgcttgcgg cgcgccgtca acgtggcgat
     1021 tccggccact cggccaggcg cgccgacacg atgtcggcga tccgcaccac gcgctgggcg
     1081 ccgtcgacct ctaccgtgac ggcgtcctcc gttactgccc ttaccttccc cttgaacgtc
     1141 tccccgccgg ccctgaactt gacgagcagg tcgtggaagc gttcgaagtg ccgcgcgcgc
     1201 cgtaacggac gctcggcgcc
//
LOCUS       DAOQYE010000827         1217 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_150956_length_1217_cov_2.555077, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000827 DAOQYE010000000
VERSION     DAOQYE010000827.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1217
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150956_length_1217_cov_2.555077"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="PKN52_11485"
     CDS             complement(<1..524)
                     /locus_tag="PKN52_11485"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNR00620.1"
                     /translation="MRLYFVRHGETDYSLVEARGARGVAKEFAPLTALGRIQIDTIAR
                     DYRLREAEAILCSTYTRALESAALLSRAINKPLYVEHDLHEWLPQKDPLAEVDPKSFE
                     RARQSLSGTRIEEDAPWESVDEVRGRVLAVLKRYRRFQTLVVVTHGVVIGSLVGVQRL
                     VDHAEIVPFDLDVDG"
     gene            673..>1217
                     /locus_tag="PKN52_11490"
     CDS             673..>1217
                     /locus_tag="PKN52_11490"
                     /inference="COORDINATES: protein motif:HMM:NF013761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HNR00621.1"
                     /translation="MDLNSLYRNVTLAIAGAGPVKSVIGRYGWRVGVGRFVAGEDIRS
                     AVPALLSIQASGRRVILDLLGEFVESESASRAVASAVRECVAVARASGVEPYFSVKPT
                     QLGLGVGLELALELADGVAGAIKDAGGHLCLDMENAPYVDGTLDLYEGLRRAGHHHVS
                     TVLQSYLHRTPEDLERLIGLAG"
BASE COUNT          189 a          411 c          415 g          202 t
ORIGIN      
        1 ccatcgacat cgaggtcgaa cgggacgatc tcggcgtggt cgacgagccg ctgcacgcct
       61 accaggctgc cgatcactac gccatgggtg acgacgacga gcgtctggaa gcggcggtag
      121 cgcttcagca ccgccagcac gcgaccgcgc acctcgtcga ccgactccca gggagcgtcc
      181 tcctctatgc gcgtgccgga caggctctgg cgggcgcgtt cgaagctctt ggggtcgacc
      241 tcggccagcg ggtccttctg aggcagccac tcgtgcaggt cgtgctcgac gtagagcggc
      301 ttgttgatgg cgcggctgag gagcgccgcg gactcgaggg cccgcgtgta cgtgctgcac
      361 aggatcgctt cggcctcgcg cagccggtag tcgcgtgcga tggtatcgat ctggatgcgc
      421 cccagggcgg tcaggggcgc gaactctttg gccacgcccc gggcgccgcg cgcctcgaca
      481 aggctgtagt cggtctcacc gtggcgtacg aagtagagtc tcacacggct cctaaagagt
      541 tgctgcctaa gcccgcgacc gggctagagc caagttatca gcggcgcgcc cggggtactg
      601 ttcgaatgtt tactcccccc tcccgtccac gcggacgtgg cctctcgcac aggtacaatc
      661 gctgcagaag gtatggatct aaactcgctc tatcgcaacg ttaccctggc aatagcaggc
      721 gctggaccag tcaagtcggt gatcggacgc tacggttggc gtgtgggcgt cgggcgcttc
      781 gtggccggtg aggacatccg ctccgccgtt ccggccctcc tctccatcca ggcgagcggc
      841 cgacgcgtca tcctggactt gctgggcgag ttcgtcgaga gcgagagcgc ctcgcgagcc
      901 gtggcttccg ccgtgcgtga gtgcgtcgcc gtcgcgcgcg cgagcggcgt cgagccgtac
      961 ttcagcgtca agccgactca actcggcctc ggggtcggcc tcgagctcgc actcgaactg
     1021 gcggacggcg tggccggcgc catcaaggac gccggcggcc acctgtgcct cgacatggag
     1081 aacgcgcctt acgtggacgg cacgctggac ctctacgagg ggctcaggcg agcgggccac
     1141 caccacgtga gcaccgtgct gcagagctac ctccaccgca cgcccgagga cctcgagcgg
     1201 ctgatcggcc tggccgg
//
LOCUS       DAOQYE010000828         1217 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_150992_length_1217_cov_2.315835, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000828 DAOQYE010000000
VERSION     DAOQYE010000828.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1217
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150992_length_1217_cov_2.315835"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1217)
                     /locus_tag="PKN52_11495"
     CDS             complement(<1..>1217)
                     /locus_tag="PKN52_11495"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HNR00622.1"
                     /translation="GLAERLGIVYTPIEVVDYILKSADHALKQEFGKSLTDEGVHILD
                     PFTGTGTFLVRLIQSGLIKLEDLPRKFSKELHANEIVLLAYYIAAINIEEAYHEQTGW
                     QYEPFEGIVLTDTFQLTEDPANGQAMTIGRVLPENSERATKQKALDITVIVGNPPYSA
                     WQESANDANQNLKYPQLDDRIASTYAEHSTATNKNSLYDSYVRAIRWASDRVGDKGIV
                     CFVTNGGFIDGNAADGLRKTLIEEFSSLYVFNLRGNARTSGEQRRKERGNVFGEGTRT
                     NIAISLLVKNPAKSTPGQLYYHDIGDYLSREEKLQTIQELGSIAETPWQSIVPNERHD
                     WINQRGDEFQAFPALGDKSGNDSFVIFTTYSNGLKTNRDAWAYNFSNAGLAANMMRMV
                     DFYNSQVEDYAAA"
BASE COUNT          181 a          309 c          407 g          320 t
ORIGIN      
        1 ggccgccgcg taatcttcga cctgcgagtt gtagaagtcg accattcgca tcatgttggc
       61 tgctaggccc gcgttcgaga agttgtatgc ccaagcgtct ctgttggtct ttagcccgtt
      121 ggaatacgtt gtgaagatga cgaaactgtc gttcccactc ttatcgccaa gcgcagggaa
      181 cgcttggaac tcgtcgccgc gttggttgat ccagtcgtgc cgctcgtttg gaacgatgga
      241 ctgccacggg gtctctgcga tgctacccag ttcctggatg gtctgaagct tctcctcgcg
      301 gctgaggtag tccccgatgt cgtggtagta gagctgcccc ggagttgact tggccgggtt
      361 cttcaccagg agactaatag caatgttggt tcgtgtgccc tcgccgaaga cgttgcctcg
      421 ttccttgcgt cgctgctccc cagaggttcg tgcgtttccg cgtaggttga acacgtagag
      481 gctgctgaac tcttcgatga gcgtcttgcg tagtccgtct gccgcgttgc cgtctatgaa
      541 gccgccgttg gtgacgaagc agacgatgcc tttgtcgcct acgcggtcgc tggcccagcg
      601 gatggcgcgg acgtaggagt cgtagaggct gttcttgttg gtcgcagtgg agtgctctgc
      661 gtaggtgctc gcaatgcggt cgtctagttg agggtacttg aggttctggt tggcgtcgtt
      721 ggcgctctcc tgccaggcgc tgtagggcgg gttgccgacg atgacggtaa tgtccagcgc
      781 cttctgcttt gtcgcccgtt cgctgttctc cggtaggacc cttccgatgg tcatggcttg
      841 gccgttggcc gggtcttccg tgagttggaa cgtgtcggtt aagacgatgc cttcgaaggg
      901 ttcgtactgc cagccggttt gctcgtggta ggcctcttcg atgttgatgg cggcgatgta
      961 gtaggcgagc aggacgatct cgttggcgtg taactccttc gagaacttgc ggggtaggtc
     1021 ttcgagcttg atgaggccgc tctggatgag tctgacgagg aaggtgccgg ttccagtgaa
     1081 ggggtcgagg atgtggacgc cttcgtcggt aaggctcttg ccgaactctt gcttgagggc
     1141 atggtctgcg ctcttgagga tgtagtcgac tacttctatg ggtgtgtaga cgatgccgag
     1201 gcgttcggcc aggccgg
//
LOCUS       DAOQYE010000829         1215 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_151342_length_1215_cov_3.760345, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000829 DAOQYE010000000
VERSION     DAOQYE010000829.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_151342_length_1215_cov_3.760345"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            794..>1215
                     /locus_tag="PKN52_11500"
     CDS             794..>1215
                     /locus_tag="PKN52_11500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00623.1"
                     /translation="MRIALRNLLTTGAVIAASLGGLAGARGADIVLPDGDTCKIVSRD
                     LDPSLANGRVSHFCSDGVALVGGLQLGDGLGLVTRVTYDPNEPQNVFSDQLVSIEVRG
                     VTLEDGTVCREAGHGATLAFDGERANFTCDDDSVLLGA"
BASE COUNT          168 a          441 c          410 g          196 t
ORIGIN      
        1 ctggccccct gcccgccttg cggcgattgg gggagcctcg ggatcttctc cgcctacccg
       61 ctcgagccag tgctggccga cctcagcggc ccggcggtac gcaacccgca ggtagccgtc
      121 gtgcaccatc cgcgtggcga cctgctcgtc gtcaacgtcc ataacctctc gacgccccgg
      181 ttcccgcaga tctggccggg tgagatcgcc cgggcagtgg agtcgaggga ggccgtggcc
      241 gccgccctcg tagagctcgt caggggtgcg agcgtgccgg tcatagcgct gggcgacttc
      301 aacaccaccg agcgcagcgg cgcctatcgg atacttacgg gcgagctact tgacgcctgg
      361 cgcaaacgcg gcttcgggtt cggcagcacg ttcaggggcg gcgcgcaagc gggcgcgggt
      421 gcgagcgcgc cggccggtgt tccggctggc gcgcaacccg gctccgctac cgtgcgaggc
      481 ggagcctcgg ccacgagcgg cgcctcgtcg agcggtctcc tcccgcgccc gaccctgccg
      541 gactggctcc tcaggatcga ctacgtgttc cattccgagg gcctcactac cctatcggtg
      601 agggtgatgc cgtggcgggc ggcatccgat caccgccccg tgaccgccct gctgggtttc
      661 gccgaccgac cccatgaacg ttcgctgaac caggctaacg gccacgcctc acgggggcgt
      721 cctaacgtgg tggtcgtcga gtcctcggcg ccgcgctcag gcgggcgacg cgcggactcc
      781 ttaggaggta gacatgcgca tcgcgcttcg caacttgctg acgaccggag cggtcatagc
      841 cgcgagcctt ggtggtctgg ccggcgccag gggcgccgac atcgtcctgc ccgacggcga
      901 cacctgcaag atagtcagtc gtgacctcga cccgagcctc gccaacggtc gcgtgagcca
      961 tttctgcagc gacggcgtcg cgctggtcgg cggcctgcag cttggcgacg gcctcggcct
     1021 cgtcacgcgc gtcacctacg acccgaacga gccccagaac gtcttctcgg atcagctcgt
     1081 ctccatcgag gtgaggggcg ttaccctcga ggacggcacc gtctgccgtg aggcgggtca
     1141 tggcgcgacc ctcgccttcg acggcgagcg cgccaacttc acgtgcgacg acgactccgt
     1201 cctcctcggc gcctt
//
LOCUS       DAOQYE010000830         1214 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_151647_length_1214_cov_2.304573, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000830 DAOQYE010000000
VERSION     DAOQYE010000830.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1214
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_151647_length_1214_cov_2.304573"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..492)
                     /locus_tag="PKN52_11505"
     CDS             complement(<1..492)
                     /locus_tag="PKN52_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit beta"
                     /protein_id="HNR00624.1"
                     /translation="MALERWFRRNRPSKKEDDNSPAGLWLKCDNCNAQIYRKDLVANS
                     YVCPECSHHHRVPADARIGMLADEGTFERWSGLIEAQDALEFVDTQPYIERLKKARQK
                     VGRPDAILTGGALLEGQPVALVVMDFSFLGGSMGSVVGEEIASATERAAAEGRTLVSV
                     TSSG"
     gene            complement(612..1148)
                     /locus_tag="PKN52_11510"
     CDS             complement(612..1148)
                     /locus_tag="PKN52_11510"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HNR00625.1"
                     /translation="MFRRRKEEAGAEPRPEPFTYIHRDTVLTGDISATGRVRVHGTVR
                     GNVGVTGVLEVAQSGLVEAQDVEADEVKIIGRVVATRVVARGKVEVWKGGELVGDVRA
                     AALDIEDGARFTGRSEMIAPTGSAAVEHAPATGAPAVDAPVVGAPVAGAAVPASTDQT
                     GRPAGRVDLDQVTEADLL"
BASE COUNT          176 a          448 c          398 g          192 t
ORIGIN      
        1 tccagacgat gtgacggaga ccagcgtccg accttctgcc gccgcgcgct ccgtggccga
       61 agcgatctcc tcgccgacga ccgaacccat cgagccgccg aggaaggaga agtccatgac
      121 cacgagcgcc accggctgcc cctcgaggag cgctccgccg gtcaggatgg cgtcggggcg
      181 tccgaccttc tggcgcgcct tcttcagccg ctcgatgtaa ggctgggtgt cgacgaactc
      241 gagggcgtcc tgggcctcga tgaggccgga ccaacgctcg aacgtaccct cgtctgcgag
      301 catgccgatg cgcgcgtcgg cgggcacccg gtggtggtgc gagcactcgg gacagacgta
      361 ggagttggcg acgaggtcct tgcggtagat ctgcgcgttg cagttgtcgc acttgagcca
      421 caaacccgcc ggactgttgt cgtcctcttt cttgctgggg cggttcctgc ggaaccaacg
      481 ctcaagcgcc acggccgccc acccctccgg cgccgggcac caagcgcgct atgtgacgtc
      541 ttggcacgcc gcatgatagc agcagccgcc cgcctaacgg gcaggacggc acgctggcgc
      601 ccatctcgcc cctagagcag gtcggcttcc gtgacctggt cgaggtcgac gcggccagcc
      661 ggtcggcccg tctggtcggt ggaggcggga acggccgcgc cagcaactgg cgcgccaaca
      721 actggcgcgt caacggctgg tgcaccagtt gctggcgcat gctcgaccgc cgcgctcccg
      781 gttggcgcga tcatctcgct cctgcccgtg aaccgcgcgc cgtcctcgat gtcgagcgct
      841 gcggccctca cgtcgccgac gagctcccct cccttccaca cctccacctt gccgcgcgcg
      901 acgacccgcg tcgcgacgac cctgccgatg atcttcacct cgtcagcctc gacgtcctgg
      961 gcctcgacga ggccggactg cgccacctcg agcacacccg tcacgccgac gttcccgcgc
     1021 accgtgccgt gaaccctgac ccggccggtg gccgagatgt cgccggtgag gacggtgtcg
     1081 cggtgtatgt acgtgaaggg ctcggggcga ggctcggccc ccgcttcctc cttgcgtcgc
     1141 ctgaacatcc ggacctcctt gcacgagccc cccgaaggcg tctgacccca agggggtagc
     1201 ggcgccggtt gagg
//
LOCUS       DAOQYE010000831         1212 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_152071_length_1212_cov_2.391530, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000831 DAOQYE010000000
VERSION     DAOQYE010000831.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_152071_length_1212_cov_2.391530"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            51..>1212
                     /locus_tag="PKN52_11515"
     CDS             51..>1212
                     /locus_tag="PKN52_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HNR00626.1"
                     /translation="MSEKRRTGVARRDGSEGLAGLGEGVPLEKRRAAPYHTNRRAWTE
                     ESERLAAERAKIAAGGGAAASARQHAKGRLTARERVARLLDPGEELAELMTFAGFGMY
                     AEEGGAPAGGVVAGIGKVAGRDWMVIANDATVKAGAFFPITAKKVIRAQTIAFENRVP
                     TIYLVDSAGVYLPMQDEIFPDQDDFGRVFYLNARMSAAGIPQLAAIMGNCVAGGAYLP
                     VMCDTLVMTEGSGLYLAGPALVRAAIGQQVESEELGGAAMHASLSGTVDFKEPDDDHA
                     IRRVRRLAATYAERPVARWAAGRKAPVAPRFDEGELTGLLPLDGGTQPYDVRQVLARL
                     LDGSELDEFKRDYGPTLVTGWGRLGGYAVGVVANQKLVIKRSRRLEVGGVIYA"
BASE COUNT          178 a          415 c          455 g          164 t
ORIGIN      
        1 ctcgagaacg cgggcggcgg cctgctggtg gtgctcgcta gttcggcgcg gtgagcgaga
       61 agcgccggac gggcgtcgcc aggcgcgacg gctcggaggg gctcgccggg ctgggcgagg
      121 gcgttccgct cgagaagcgc cgcgcggcgc cgtatcacac gaacaggcgc gcgtggactg
      181 aagaaagcga gcggctagcc gccgagcgcg cgaagatcgc ggccggcggc ggggcggcgg
      241 cgagcgcccg acagcacgcc aagggtcggt tgacggcccg cgagcgcgtc gctcgcctcc
      301 tcgacccggg cgaggaactg gccgagctca tgaccttcgc gggcttcggc atgtacgccg
      361 aggagggcgg agcgccggcg ggcggcgtgg tcgcgggcat aggcaaggtg gccggccgcg
      421 actggatggt catcgccaac gacgcgaccg tcaaggcggg ggctttcttc cccataacgg
      481 cgaagaaggt gatccgcgcc cagaccatcg cgttcgagaa ccgcgtcccg accatctacc
      541 tggtcgactc cgcaggcgtc tacctgccta tgcaggacga gatcttcccc gaccaggacg
      601 acttcggccg cgtgttctac ctcaacgccc gcatgagcgc ggccggcatc ccgcaactcg
      661 ccgccatcat gggcaactgc gtggccggcg gcgcctacct gcccgtgatg tgcgacacgc
      721 tggtgatgac cgagggctcc gggctctacc tggccgggcc ggccctggta cgtgcggcca
      781 tcggtcagca ggtggagagc gaggagctcg gcggggccgc catgcacgcg tccttgtccg
      841 gcaccgtcga cttcaaggag ccggacgacg accacgccat ccggcgtgtg cggcggttgg
      901 ccgccaccta cgccgagcgg cccgtggccc gctgggccgc cggtcgcaag gcgccggtcg
      961 cgccgcgctt cgacgagggc gagctgaccg gtctgttgcc cctggacggc ggcacccagc
     1021 cctacgacgt caggcaggtg ctggcgcggc tcctcgacgg ctcggaactc gacgagttca
     1081 agcgcgacta cggcccgacc ctcgtcacgg gctgggggcg cttgggcggc tacgccgttg
     1141 gcgtagtggc gaaccagaag ctagtgatca aacgctctcg ccgtctcgag gtcgggggcg
     1201 tgatctacgc cg
//
LOCUS       DAOQYE010000832         1210 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_152534_length_1210_cov_1.949784, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000832 DAOQYE010000000
VERSION     DAOQYE010000832.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1210
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_152534_length_1210_cov_1.949784"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1096
                     /locus_tag="PKN52_11520"
     CDS             <1..1096
                     /locus_tag="PKN52_11520"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNR00627.1"
                     /translation="VGGLLAYFGLRFLYQWLYEALFELPLGDYLIVGAILAVIVVAGV
                     LPGVGVGLVLTVALFVISSSRTDAVRYAVGGADLRSRVTRGAAELARLAEHGGEIMVV
                     QLQGFLFFGTATALLERLERRVSAGPPVRSVILDFSRVSGVDATGLATIADAFRLGEA
                     SGFELLLSGVPDRIYRRLGRSRKGGRDAVVEERFAKLDAALEHAEERLLERWDARRDV
                     RGTEVDAVAHYSGTGFDFADLLPYLTRRELRAGEVLIRKGDKADELYFLVDGQMTAVA
                     EGSDGAATRLETLRSGGLLGELGFYRGGVRSATVRADHDSKLLVLDERGLTRLTAEAP
                     QLAADLHRQVARLMAGRVLHLMAAVDALER"
BASE COUNT          155 a          424 c          446 g          185 t
ORIGIN      
        1 cgtcggcggc ctcctggcgt acttcggctt gaggttcctc taccagtggc tctacgaggc
       61 gctcttcgag ctgccgctcg gcgactacct catcgtgggg gcgatcctcg cggtcatcgt
      121 cgtggcggga gtgctgccgg gcgtcggcgt cgggctcgtc ctcacggtcg ccctgttcgt
      181 catcagctcg agccggaccg acgccgtcag gtacgcggtc ggcggcgccg acctgcgttc
      241 gcgcgtgacg cgcggcgcgg cggagctggc gcgtctcgcc gagcacggcg gcgagatcat
      301 ggtcgtgcag ctccaaggct tcctgttctt cggcacggcc acggcgctcc tggagcggtt
      361 ggagcgccgc gtgagcgccg ggccccccgt gcgttccgtg atcctcgact tctcgcgcgt
      421 gtccggcgtg gacgccacgg gccttgctac gatcgcggac gccttcaggt tgggcgaagc
      481 gtccgggttc gagctcctac tgagcggcgt acccgaccgc atctaccggc gcctcggccg
      541 gtcgcgtaaa gggggcaggg acgcggtcgt cgaggagcgt ttcgctaagc tcgacgccgc
      601 gctcgaacac gccgaggaac gcctgctcga gcgttgggac gcgaggcgcg acgtccgcgg
      661 caccgaggtg gacgccgtcg cccactactc cggcaccggc ttcgacttcg ccgacctgct
      721 cccgtacctg acgcggcggg agctgcgggc gggcgaggtg ctcatccgca aaggcgacaa
      781 ggcggacgaa ctctacttcc tggtcgacgg acagatgacg gcagtggccg agggtagcga
      841 cggcgccgcg acgcgcctcg agacgctgcg ttccggcggt ctgctgggcg agctcggctt
      901 ctaccgcggc ggcgtgcgga gcgccaccgt gcgcgccgat cacgactcga agctgctcgt
      961 actcgacgaa cgggggctga caaggctcac ggcggaggcg ccgcagctcg cggccgacct
     1021 gcaccggcaa gtcgcgcggc tcatggccgg ccgcgtgctg cacctcatgg cggccgtcga
     1081 cgccctggaa cgttaggcgg ccagctcctc gaccagacgg gcgccgaggg cctcggcgag
     1141 cgtggcggcg cgcaggaacc cgcggccccc caacgccccg aagatgtgga cgttgcgcgt
     1201 gaggcgcctg
//
LOCUS       DAOQYE010000833         1209 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_152641_length_1209_cov_3.023397, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000833 DAOQYE010000000
VERSION     DAOQYE010000833.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1209
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_152641_length_1209_cov_3.023397"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..853
                     /locus_tag="PKN52_11525"
     CDS             <1..853
                     /locus_tag="PKN52_11525"
                     /inference="COORDINATES: protein motif:HMM:TIGR01376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00628.1"
                     /translation="SMIVGSSRCAISDVTFIFKGAGIFNGGTLTVRNTDILDSRAWEG
                     GGGIWNSATGNLRLEQVVLSGNEAAVLDSEDGNPLVIRDQVMSGQSFNLQGYGGALFN
                     DGGTVYMEDTTVSSNRSRFSGGAVYSNGGSLEIVDSTMTGNSSNFAYYTPGGNQPGFG
                     GGIVLTNSATLTITSVTFSGNFAQREGGALYMDAGASVVHSGVAMNNNSADYGGGISQ
                     KYYQAVGELDRIGMTFSGNTATSLGPDYYGVCLDCLPVAGATRHGILDLPSQGLYTPS
                     TDSSRFR"
     gene            933..>1209
                     /locus_tag="PKN52_11530"
     CDS             933..>1209
                     /locus_tag="PKN52_11530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00629.1"
                     /translation="MIESKQCVNLPNDPQHVKPRNRKGVGFRVALLLLLFVALWGCAP
                     VSPSGEAEPPEPRKVEWRASDIGTVTLGGAFEFLDRNLGDFVLSGSGL"
BASE COUNT          232 a          370 c          377 g          230 t
ORIGIN      
        1 cagcatgatc gtgggttcga gccgctgtgc gatcagcgac gtcaccttca tcttcaaggg
       61 ggccggcata ttcaacggcg gcaccttgac cgtccggaac accgacatcc tggacagtag
      121 agcctgggag ggcggcggtg gcatctggaa ctcggcgaca ggcaacctgc gcctggaaca
      181 ggtcgtgctg agcgggaacg aggcggcggt gctcgattcc gaggacggca accctctggt
      241 gatccgcgac caggtcatgt ctggtcagtc gttcaacctg cagggctacg gtggcgcgct
      301 cttcaacgac ggcggaacgg tctatatgga ggacaccacc gtgagttcga accgctcccg
      361 cttctctggg ggggccgtct actccaacgg cggaagccta gagatcgtcg attccacgat
      421 gaccggcaac tcatcgaact tcgcctacta cacgcctggc ggcaaccagc ccggcttcgg
      481 tggcggcatc gttctcacga actctgcgac gttgaccatc acctcggtca ccttctccgg
      541 caacttcgct caacgcgaag gtggggcgct ctacatggac gctggggctt cggtcgtgca
      601 ttccggcgtg gcgatgaaca acaacagcgc ggactatggt ggtgggataa gccagaagta
      661 ctaccaggcg gtaggcgagc tcgaccgaat agggatgacg ttcagcggta acactgctac
      721 cagcctcggg cccgattact acggcgtctg ccttgactgc ttacccgtcg cgggcgcgac
      781 tcggcatggc atcctggatc tgccgtccca aggcctatat accccttcga cggacagctc
      841 gcgtttcaga tgagacggga gcgctagccc taagacggtt cacatcctgg ctgtgtatgg
      901 tcaataccaa tgtgcgcagc cggatgtgaa ccgtgatcga aagcaagcag tgcgttaacc
      961 tgccgaacga cccacaacat gtaaagccgc ggaaccgcaa gggggtaggc tttcgcgtgg
     1021 cgttacttct ccttcttttc gtcgccctgt ggggctgcgc gccggtatcg ccttccggcg
     1081 aggccgaacc tcccgagccg aggaaggtcg agtggcgcgc ctccgacatc ggaaccgtga
     1141 cgctgggcgg cgccttcgag ttcctagaca ggaacctcgg cgactttgtc ctatctggaa
     1201 gcggactcg
//
LOCUS       DAOQYE010000834         1209 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_152693_length_1209_cov_2.421144, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000834 DAOQYE010000000
VERSION     DAOQYE010000834.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1209
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_152693_length_1209_cov_2.421144"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..743)
                     /locus_tag="PKN52_11535"
     CDS             complement(<1..743)
                     /locus_tag="PKN52_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNR00630.1"
                     /translation="MSLLIKNGEIVTAAERMHADIYCEGETITRIGKGLDAPEGTTVV
                     DAAGKYVFPGFIDPHVHIHLPFMGTLTKDTHRTGSQAALVGGTTTYIEMLAPSRHEDL
                     MAGYRLWNELAEGNSACDYTFHIGVSKYDAEAEAQLREIVADGMTSFKVFLSYKGFFG
                     VNDEELFRTLTLAKELGVRVTAHCENDELVAQLQAQLLAAGKTGPEWHEPSRPERVEA
                     DGTGHFATFLEITGAKGYVVHLSCEPALEA"
     gene            complement(813..>1209)
                     /locus_tag="PKN52_11540"
     CDS             complement(813..>1209)
                     /locus_tag="PKN52_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNR00631.1"
                     /translation="HRIGHFGDFDLSFKTVAVIDEEKCIKCNLCYVACDEGAHQCIDL
                     TKDGVRVDPEIHLGAAGLLQPKVREEDCVGCDLCSLVCPVDDCITMQERPRERDSFTW
                     NELVRTRPEVTTDWDAMVAFRKEVGIEIH"
BASE COUNT          168 a          399 c          414 g          228 t
ORIGIN      
        1 gcctccaggg cgggctcgca gctcaggtgc acgacgtagc ccttggcgcc cgttatctcg
       61 aggaaggtgg cgaagtgacc cgtgccgtcg gcctcgaccc gctcgggccg cgagggctcg
      121 tgccactcag gcccggtctt gcccgcggcg agcagctgcg cctgcagctg ggccaccagc
      181 tcgtcgttct cgcagtgcgc cgtgaccctg acgccaagct ccttggcgag ggtgagggtg
      241 cggaagagct cctcgtcgtt gacgccgaag aagcccttgt aggagaggaa gaccttgaag
      301 gacgtcatgc cgtccgcgac gatctcgcgc agctgcgcct ccgcctcggc gtcatacttc
      361 gacacgccga tgtggaacgt gtagtcgcag gcgctgttgc cttccgccag ctcgttccag
      421 aggcggtagc cggccatgag gtcctcgtgc cggctgggcg ccagcatctc gatgtaggtg
      481 gtcgtgccac cgacgagcgc ggcctggctg cccgtgcggt gtgtgtcctt ggtgagggtg
      541 cccatgaacg gcagatggat gtgcacgtgc gggtctatga agccggggaa cacgtacttg
      601 ccggccgcgt cgacgaccgt cgtgccctcc ggcgcgtcca gccccttgcc gatgcgcgtg
      661 atggtctcgc cctcgcagta gatgtcggcg tgcatgcgtt cagccgccgt gacgatctcg
      721 ccgttcttga tgagcaggct cacgttcgtc ctttcacgtc gtaactcgcg gggcgccgcc
      781 cagcgcgtgc gcgccggcgc ccgctaggcg cgtcagtgga tctctatgcc cacttccttg
      841 cggaacgcga ccatggcgtc ccagtccgta gtcacttccg gccgcgtcct gacgagctcg
      901 ttccaggtga agctgtcgcg ctcgcgcggt cgctcctgca tggtgatgca gtcgtcgacg
      961 gggcatacga ggctgcagag gtcgcagccg acgcagtcct cctcgcgcac cttcggctgg
     1021 agcagcccgg cggcgccgag gtggatctca gggtcgaccc gcacgccgtc cttggtgagg
     1081 tcgatgcact ggtgcgcgcc ctcgtcgcag gcgacgtagc agaggttgca cttgatgcac
     1141 ttctcctcat cgattactgc gacggtcttg aacgagaggt cgaagtcgcc gaagtggccg
     1201 atgcggtgc
//
LOCUS       DAOQYE010000835         1203 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_153927_length_1203_cov_2.992160, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000835 DAOQYE010000000
VERSION     DAOQYE010000835.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1203
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_153927_length_1203_cov_2.992160"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(35..733)
                     /locus_tag="PKN52_11545"
     CDS             complement(35..733)
                     /locus_tag="PKN52_11545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00632.1"
                     /translation="MNDKRARADVDGSAALRLEELLAVAIGEADPTLPERLEHDPEAY
                     LDLIRLGARAAAYSDELLARAVSSARAAGLSWEAIGAELGVSKQAVHKRFGASSAGPA
                     DDRGAVAAERGSAVAPARPGGAGGSDEPTGTTQLVLSPLTAFNEMEALERVGMYGWHS
                     VGYGPLYHLVVKSPRRWQHARVATFGGGSTSFEEHGWQRVGKSWFPWSYYKRELMEEA
                     LPEPPGWSPLAGVR"
     gene            complement(726..980)
                     /locus_tag="PKN52_11550"
     CDS             complement(726..980)
                     /locus_tag="PKN52_11550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00633.1"
                     /translation="MKSPLRDRSLTASLLAGFAVSSVALSLLGVVLAPGPARGLATVS
                     AVISVGIAGGTLARLVHAGWFDGDTVPRRGGRSREEVRDE"
     gene            1076..>1203
                     /locus_tag="PKN52_11555"
     CDS             1076..>1203
                     /locus_tag="PKN52_11555"
                     /inference="COORDINATES: protein motif:HMM:NF014588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HNR00634.1"
                     /translation="MPPALRIGHGQDAHRLAPGRPLVVGGVAVSSERGADAHSDGDV"
BASE COUNT          185 a          450 c          398 g          170 t
ORIGIN      
        1 ggtggccaga gcgacccggt cgcgcacgta gcgttcagcg cacgcccgcg agcggactcc
       61 aacccggcgg ctctggcaac gcctcttcca tcagctcacg cttgtagtag ctccacggga
      121 accaactctt gccgacgcgc tgccagccgt gctcctcgaa cgacgtggaa ccgcccccga
      181 acgtggcgac gcgggcgtgt tgccaccgcc tcggcgactt gacgaccagg tggtaaagcg
      241 ggccgtaacc cacggagtgc cacccgtaca tgcccacgcg ttcgagcgct tccatctcgt
      301 tgaaggccgt aaggggtgag aggaccagct gcgtggtccc cgtcggctcg tccgagcctc
      361 ccgcgcctcc aggcctagct ggggcgaccg cgctcccgcg ttcggccgct acggctccgc
      421 ggtcgtcggc cggaccggcc gaagacgcgc cgaagcgctt gtgaaccgcc tgcttgctca
      481 cccccagctc cgctccgatg gcctcccaac tgaggccggc ggcacgggcg gaactcacgg
      541 cgcgcgcgag gagctcatcc gagtaagccg ccgcgcgcgc gccgagccgg atgaggtcga
      601 ggtaggcctc ggggtcgtgt tccaggcgct ccggcagcgt tgggtcggct tcaccgatcg
      661 cgaccgccaa cagctcctcg aggcggagcg ccgcggaacc gtcgacgtcg gcacgggcac
      721 gcttgtcatt catcgcggac ctcctcccgg gaccgtccgc cccgcctcgg aacagtgtcc
      781 ccatcgaacc aaccggcatg caccaggcgc gccagcgtgc cgcctgcgat accgaccgag
      841 atcaccgcgg acacggtcgc gagaccgcgc gctgggccag gcgccagtac gacgccgagc
      901 agcgacaggg ccacggatga cacggcgaaa ccggccagca acgatgccgt gaggctacga
      961 tcccgcagcg ggctcttcat gcgtcaatga taggttgaca ccacgtcatt gtcaatcttc
     1021 tgttgacgcg cccgcccggc aggggtcgcc tcgcgcccgc tgctaggatg cgcccatgcc
     1081 cccagccctc cgcatcggtc acggtcagga tgcccaccgc ctcgcgccgg gcaggccgtt
     1141 ggtggtcggc ggggtggcgg tctcgtccga gcgcggcgcc gacgctcatt cggacggcga
     1201 cgt
//
LOCUS       DAOQYE010000836         1202 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_154231_length_1202_cov_1.952049, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000836 DAOQYE010000000
VERSION     DAOQYE010000836.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1202
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_154231_length_1202_cov_1.952049"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1144)
                     /locus_tag="PKN52_11560"
     CDS             complement(<1..1144)
                     /locus_tag="PKN52_11560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00635.1"
                     /translation="MIAERRAEALNQKGITVDRFSGPVGELVPQPPRRRESTSTSGSG
                     AGAPPSRASGSGGRGVPSGAKAPARGDAWGELPAQPGEGTPAQPEESVRAVTRVRLEG
                     GVPAGTVGAARPKELASAHDVWARRQHARWQPLDTIVAAKHDPRVFDADFPDTVVRAF
                     RRGPNGSLRPEPALVKDETVQATRPAQPVERGSRTRSAPSTRQEPTQAPRGVPTSAAS
                     GERVLHARDAGDRFEPAYREDRSYRDEPAYGADQPGHREHAADMFESPLAAAAADQAG
                     LRRAGFNAGFDADLDADLDGGPDERADLDHLVEEAPAKSPLKGVIARLSGLRFALGRE
                     RGAGDGSHGGAHDGSHGYARADGRADAHADTHDLDAGPVRGSERITV"
BASE COUNT          133 a          433 c          467 g          169 t
ORIGIN      
        1 agacggtgat gcgttcggaa cccctcacgg ggccggcgtc caggtcgtgg gtgtctgcgt
       61 gggcgtctgc tctgccgtcc gcgcgcgcgt acccgtggga gccgtcatgg gcgccaccgt
      121 gcgagccgtc cccggcgccg cgctcgcgac ccagcgcgaa gcgcaggccg ctcaaccggg
      181 cgatgacgcc cttgagcggg ctcttggccg gcgcctcctc gacgaggtgg tcaaggtcgg
      241 cgcgctcgtc ggggccgcca tcgaggtcgg catccagatc agcatcgaag ccggcattga
      301 agccggcgcg cctgaggccc gcttggtctg cggccgccgc ggcgagcggg gactcgaaca
      361 tgtcggcggc atgttcccgg tgaccgggct ggtcagcgcc gtaggcgggc tcgtcgcggt
      421 aactccggtc ttcgcggtag gcgggctcga accgatcgcc cgcgtcacgc gcgtgcagga
      481 cgcgctcgcc ggacgccgcg ctggtcggca cgccgcgcgg ggcttgcgtg ggctcttgcc
      541 tggtcgacgg cgcgctccgc gtccgggagc cgcgctcgac cggctgcgcg gggcgagtgg
      601 cctggaccgt ctcgtccttg acgagggccg gctcgggcct cagggagccg ttcggaccgc
      661 gcctgaacgc gcggacgacc gtgtcgggga agtcggcgtc gaagactcgc gggtcgtgct
      721 tggcggcgac gatcgtgtcg agcggttgcc agcgggcgtg ctggcggcgc gcccagacgt
      781 cgtgcgccga agccaactcc ttcggccgcg cagcgccgac ggtgccagcg ggaacgccgc
      841 cctcaagccg gaccctggtt acggcccgga cggactcctc gggttgggcg ggagtgcctt
      901 cgccgggctg cgcgggcaac tcgccccacg cgtcgccgcg cgcgggcgcc ttcgcgccgc
      961 tcggcacgcc ccgaccaccg gatccgctgg cgcggctagg tggagcgcca gcgcccgagc
     1021 ccgaggtgga ggtcgactcc cgccgcctcg gcggctgcgg gacgagctcg ccgacgggac
     1081 cggagaagcg gtcgaccgtg atgcccttct ggttgagggc ttccgccctg cgttcggcga
     1141 tcacctgcgc ctcttggcgc tccacggcgg cctgccagtc agagaccgag ccgcgggcgg
     1201 ca
//
LOCUS       DAOQYE010000837         1201 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_154471_length_1201_cov_2.047993, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000837 DAOQYE010000000
VERSION     DAOQYE010000837.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1201
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_154471_length_1201_cov_2.047993"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..877)
                     /locus_tag="PKN52_11565"
     CDS             complement(<1..877)
                     /locus_tag="PKN52_11565"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HNR00636.1"
                     /translation="MSAEPRALDMILAKRAGRAHSAAELEAFLAGYVNGTVPDYQVAA
                     WLMAVCWRGMTDDETAALTMAMARSGDVLDLGDLPNTVDKHSTGGVGDKTTLVLAPLL
                     AALGGTVAKMSGRGLGHTGGTVDKLESIPGFRASLSDEEFRRVAREAGVVVTGQSKDL
                     APADGLLYALRDTTGTVESLPLIASSVMSKRLAGGAKSIVLDVKVGSGAFMKTLDDAR
                     ALATAMVAIGKLAGRNVRAILSGMGQPLGRMVGNAMEVEEAVACLRGEGPTDLLELCL
                     VLAEEAAVRLLALPAV"
     gene            complement(952..>1201)
                     /locus_tag="PKN52_11570"
     CDS             complement(952..>1201)
                     /locus_tag="PKN52_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramyl peptide synthase"
                     /protein_id="HNR00637.1"
                     /translation="SIDAILAEIAAGAAAAGGRAGVDYLLEPDRPTAIRRAVRLARPG
                     DVVLLAGKGHERTLERASETLAWDEASEARAALAELGG"
BASE COUNT          155 a          460 c          416 g          170 t
ORIGIN      
        1 cgaccgctgg gagagcgagg aggcgtacag cggcttcctc ggcgagcacg aggcatagct
       61 ccaggaggtc ggtcgggccc tcgccgcgca ggcaggcgac ggcctcctcg acctccatgg
      121 cgttcccgac catccgcccg aggggctgcc ccatccccga caggatggcg cggacgttgc
      181 gcccggcgag cttcccgatc gcgaccatgg ccgtcgccag cgcgcgcgcg tcgtccagcg
      241 tcttcatgaa cgcgccgctg ccgaccttga cgtctagcac gatggacttg gcaccgcccg
      301 cgagcctctt gctcatgacg ctgctggcga tgaggggcaa cgactcgacc gtgcccgtcg
      361 tgtcgcgcag cgcgtagagc agcccgtccg ccggggcgag gtccttcgac tgcccggtca
      421 cgaccacgcc ggcctcgcgc gccacgcgcc tgaactcctc gtcgctcagg ctcgcgcgga
      481 agccggggat gctctcgagc ttgtcgacgg tgccgccagt atgcccgagc ccgcgcccgc
      541 tcatcttcgc gacggtgccg cccagcgcgg ccaggagcgg cgccagcacg agcgtggtct
      601 tgtcgcccac gccgcccgtc gagtgcttgt cgaccgtgtt cggcaggtcg ccgaggtcga
      661 gcacgtcgcc ggagcgagcc atcgccatgg tcaacgccgc cgtctcatcg tcggtcatgc
      721 cgcgccagca cacggccatc agccaggccg ccacctggta gtcgggcacc gttccgttca
      781 cgtagccggc caggaacgcc tcgagctcgg ccgccgagtg ggcacggccg gcgcgcttgg
      841 ccaggatcat gtcgagggcg cgcggttcgg cgctcacgcc gtcacgcccc cgtcggtgac
      901 gagtcttcgc atgcgccaac tgtagcgaat gcgctaaagg cgcgtggtcg ctcagccccc
      961 cagctcggcc agggcggcgc gggcctcgct cgcctcgtcc cacgcgagcg tctcgctcgc
     1021 gcgctccagc gtgcgctcat gccccttgcc cgcgagcagc acgacgtcgc cgggccgggc
     1081 cagccggacg gcgcggcgga tggccgttgg ccggtccggt tccaggaggt agtccacgcc
     1141 agcacggccg cccgccgcag cggcgccggc cgcgatctcg gcgaggatgg cgtcgatgct
     1201 c
//
LOCUS       DAOQYE010000838         1197 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_155263_length_1197_cov_2.945709, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000838 DAOQYE010000000
VERSION     DAOQYE010000838.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1197
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_155263_length_1197_cov_2.945709"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..631
                     /locus_tag="PKN52_11575"
     CDS             <1..631
                     /locus_tag="PKN52_11575"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNR00638.1"
                     /translation="RVVTRLARPESGACAIDDVDVSRLSRAALARKVALVAQEPEVPV
                     GYRVREVVAMGRAPHLGLFGSPRAGDEAIVERAMAVTETAAFAERPIETLSGGERQRV
                     VFARALAQEPRYLLLDEPTNHLDLRFQVELLRHARTQAASGLGVLLVIHDLNLAARAC
                     DRLVVLDGGRVVAEGAPRDVLLPETLESVFRADVDVMPGPTGPVVVARV"
     gene            complement(717..>1197)
                     /locus_tag="PKN52_11580"
     CDS             complement(717..>1197)
                     /locus_tag="PKN52_11580"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNR00639.1"
                     /translation="TRLPGVRLAMVGSGPAEADLRRRFKGTNTVFTGYMSGLELAAAY
                     ASSDVFAFPSDTETLGFVAMEAMASGVPAVGARAGGIPDVIKDGENGLLFTPGDLGDL
                     TDKLGSLLFDPALRAKLAASARADMERHGWRAATQALVDYYDLAIKVRERFDPPSVY"
BASE COUNT          153 a          416 c          449 g          179 t
ORIGIN      
        1 gcgcgtcgtc acgcggctcg cgaggccgga gtcgggagcg tgcgccatcg atgacgtgga
       61 cgtctcgcgc ctgtcgcgtg ccgccctcgc ccgcaaggtc gcgctcgtcg cccaggagcc
      121 tgaggtgccg gtcgggtacc gcgtgcgcga ggtggtggcc atgggccgcg cgccgcacct
      181 cggtctgttc ggttcgccga gggcgggcga cgaggccatc gtcgagcgcg ccatggccgt
      241 cacggagacg gccgccttcg ccgagcgccc gatcgagacg ctgtctggcg gtgagcggca
      301 acgggtcgtg ttcgctcgcg ctcttgcgca ggagccgagg tacctgctcc tggacgagcc
      361 gaccaaccac ctcgacttgc gctttcaggt ggagctgctg aggcacgcgc ggacgcaggc
      421 cgcgtcaggc ttgggggtgc tgctggtgat ccacgacctg aacctggcgg cccgtgcctg
      481 cgaccgcctc gtcgtgttgg acggcggccg cgtcgtagcc gaaggtgcgc cccgcgacgt
      541 gctgctgccg gagactctcg agagtgtgtt ccgcgcagac gtcgacgtta tgccggggcc
      601 gaccgggccg gtcgtcgtcg ctcgggtctg atacgccgtg gcgggcgcct ctggcgggcg
      661 ccccccgggg gtcgcgtgtt cgtcgcgaac gccgggcagc aggcgggtcg ccgcgctcag
      721 tagacgctcg gcgggtcgaa ccgctcgcgc accttgatgg cgaggtcgta gtagtccacc
      781 agcgcctgag tggcggcgcg ccacccgtgc cgctccatgt cggcgcgcgc gctcgcggcc
      841 aacttggccc gcagagcggg atcgaagagc aggctgccaa gcttgtcggt cagatcgccc
      901 aggtcgcccg gcgtgaacag gagaccgttc tcgccgtcct tgatgacgtc cgggatgccg
      961 ccggcgcgcg cgccgacggc cggcacgccc gaggccatgg cctccatcgc gacgaagccg
     1021 agcgtctctg tgtcggatgg gaaggcgaag acgtccgagc tcgcgtaggc ggccgccagc
     1081 tcaagcccgg acatgtaacc ggtgaacacc gtgttagtgc ccttgaagcg gcgcctgagg
     1141 tccgcctcgg cgggacccga acccaccatg gccaggcgca cgcccggcag ccgcgtg
//
LOCUS       DAOQYE010000839         1196 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_155475_length_1196_cov_2.842244, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000839 DAOQYE010000000
VERSION     DAOQYE010000839.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1196
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_155475_length_1196_cov_2.842244"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(149..469)
                     /locus_tag="PKN52_11585"
     CDS             complement(149..469)
                     /locus_tag="PKN52_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HNR00640.1"
                     /translation="MDEGFRQAVLRVVRSVPEGKVTTYGEVALLAGRPNHARQVGAIL
                     FGLRDDEADDVPWQRVINASGGISTYKVGSGELQVALLRSEGVEVINDRVDLRRYRWL
                     GEAE"
     gene            647..823
                     /locus_tag="PKN52_11590"
     CDS             647..823
                     /locus_tag="PKN52_11590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00641.1"
                     /translation="MLTLTVQVASYVFLVGVLLLFSSASPKVREWFPRGFAVGSLLAL
                     GSFLVVLVGALVFR"
     gene            853..>1196
                     /locus_tag="PKN52_11595"
     CDS             853..>1196
                     /locus_tag="PKN52_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178879.1"
                     /note="catalyzes the formation of
                     2-methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at
                     position 37 in tRNAs that read codons beginning with
                     uridine from N6-(dimethylallyl)adenosine (i(6)A); Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HNR00642.1"
                     /translation="MRAHVITFGCQMNEYDTHTIQSELVAGGHALVAHPADADLLLLN
                     TCAVRGKPVEKVISVLGDLRKQRTEGRDVTVGLMGCLAQLDEGREVARKFGVDLLVGP
                     GAITDLLEALDE"
BASE COUNT          170 a          425 c          408 g          193 t
ORIGIN      
        1 cggcgacagg ctctacgtcg gctgaggctc ccgaccggcc gaggctctcg accgcggagg
       61 ctctcgaccg gctgaggctc tcgaccggtc gagaggcgag gttcgaccga ggcgccggtg
      121 cgggcgaggt cttcggtcgg tcgcgccgct actccgcctc gcctagccac cgatagcgcc
      181 gcaggtcgac gcggtcgttg atcacctcga cgccctctga acgcaggagg gcgacctgaa
      241 gctcgccgga gccgaccttg taggtagaga tgccgccgct cgcgttgatg acacgctgcc
      301 acggcacgtc gtccgcctcg tcgtcgcgca ggccgaagag gatcgcgccg acctggcgcg
      361 catggttcgg acgccccgcc aggagcgcca cctcgccgta ggtcgtcacc ttaccctccg
      421 gaacggaacg cacgacgcgt aggaccgcct gcctgaaccc ctcgtccatg ccagatgcta
      481 acgcccgtaa gggcgtcatg ccggcaggaa ccgatcccgg tgcgaccgcc gtgcggcgcc
      541 gtgcggcgcc gcgcgggctc cgacgatcgc cggagccaca gacgtccggt gccgcgaagc
      601 gtgcggcgac cggccgcttc cgttatcctc tccatcggag gcgcgagtgc tgacgttgac
      661 ggtgcaggtc gccagttacg tgttcctggt cggcgtgttg ctgctgttct cgagcgcctc
      721 gcccaaagtg cgcgagtggt tcccgcgcgg cttcgccgtc ggttcgctcc tcgcgttggg
      781 cagcttcctg gtggtcctcg tcggcgccct ggtgttccgc tgaggcgtcc ggcgcggcga
      841 ttggagcccg agttgcgagc gcacgtcatc accttcgggt gtcagatgaa cgagtacgac
      901 acccacacca tccagtccga gctggtggcg ggcgggcacg cactcgtggc gcaccctgcc
      961 gacgccgacc tgctcctcct caacacctgc gccgtgcgcg gcaaacccgt cgagaaggtc
     1021 attagcgtgc tcggggacct gcgcaagcag cgcacggagg gccgcgacgt caccgtcggg
     1081 ctcatgggct gcctcgccca gctcgacgag gggcgcgagg tggcgcggaa gttcggcgtc
     1141 gacctactcg tcggacctgg cgccatcacc gacctcctag aggctcttga cgaata
//
LOCUS       DAOQYE010000840         1194 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_155868_length_1194_cov_3.350307, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000840 DAOQYE010000000
VERSION     DAOQYE010000840.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_155868_length_1194_cov_3.350307"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /locus_tag="PKN52_11600"
     CDS             <1..591
                     /locus_tag="PKN52_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HNR00643.1"
                     /translation="QMLESMITNPTPTRAEASDVANAIFDGTDAVMLSAETAVGAYPV
                     EAVRMMDRIARAVEASPEYRDAMHQLIPTADASTADAVALAAVEMAYNLDARLIVTFT
                     SSGNTALRVSRNRPPTPVLAITPNPRALRQMMVAWSVVPYLSEDIHNTDEMVVVAKRG
                     IEATELIGRDDRFIITAGVPFGMRGTTNLIRVERNR"
     gene            complement(743..>1194)
                     /gene="rplI"
                     /locus_tag="PKN52_11605"
     CDS             complement(743..>1194)
                     /gene="rplI"
                     /locus_tag="PKN52_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HNR00644.1"
                     /translation="NVILLEPVEKLGEAGDIVRVKDGYARNFLVPQGLALPATKSNQA
                     ELQARLAQRARQLAERKSDAERLAAMLGDAKVEIRVKAGEGRIYGSVGNRDIVDALKA
                     AYDVEIDRRKVVLAEPIKVTGEYSVPYRPHPEVTIDLKVLVVAEGEA"
BASE COUNT          200 a          405 c          411 g          178 t
ORIGIN      
        1 cagatgctcg agtcgatgat cacgaacccg acgcccacgc gggcggaagc ttccgacgtc
       61 gcgaacgcca tcttcgacgg caccgacgcc gtgatgctct cggccgagac ggcggtgggc
      121 gcctaccccg tcgaggcggt acgcatgatg gaccgtatcg ccagggcggt cgaggcgtcg
      181 cccgagtacc gggacgccat gcaccagctg ataccgacgg ccgacgcctc gacggccgac
      241 gccgtggcgc tggcggcggt cgagatggcg tacaacctcg acgcgcggct tatcgtcacg
      301 ttcacgtcga gcggcaacac ggcgctgcgc gtgtcacgca accgcccgcc gacgccggtc
      361 ctcgccataa ccccgaaccc gcgggcgctc agacagatga tggtcgcctg gagcgtcgtc
      421 ccctacctga gcgaggacat ccataacacc gacgagatgg tggtcgtggc caagcgcggc
      481 atcgaggcga ccgagctgat aggacgcgac gaccgcttca taatcacggc cggtgtgccg
      541 ttcggcatgc gtggcacgac caacctcatc cgcgtggagc gcaaccgctg agtagaccag
      601 acgccgaagg cgtccggtcg ccgcggtagc caccgaaccc cgaagcgagg caagcgcgcc
      661 tcgagtgcgc cccaagcgaa cgccgcggcc ggtaagtcac cggccgcggc gcgtcagtcg
      721 atctggcgga ctagcccgct actcaggctt cgccttcggc cacgacgagc accttgaggt
      781 cgatggtgac ctcggggtgc gggcggtacg ggaccgagta ctcgccggtc accttgatgg
      841 gctcggcgag gacgaccttg cggcggtcga tctcgacgtc gtaggcggcc ttgagggcgt
      901 cgacgatgtc gcggttgccg acagagccgt agatacggcc ttcgccggct ttgacgcgga
      961 tctcgacctt ggcgtcgccg agcatggcgg cgaggcgttc cgcgtcggac ttgcgctccg
     1021 ccaactggcg ggcgcgctga gccaggcgcg cctggagttc agcctggttc gacttggtcg
     1081 cgggcagggc gaggccctga ggaaccagga agttccgggc gtaaccgtcc ttgacgcgga
     1141 cgatgtcgcc tgcttcgccc agcttctcaa cgggctccag caggatcacg ttca
//
LOCUS       DAOQYE010000841         1189 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_157192_length_1189_cov_1.751323, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000841 DAOQYE010000000
VERSION     DAOQYE010000841.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1189
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_157192_length_1189_cov_1.751323"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..633
                     /locus_tag="PKN52_11610"
     CDS             <1..633
                     /locus_tag="PKN52_11610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00645.1"
                     /translation="DLDGYSLVSRVKRVARLARIPVILTTEADAGLGLSQRTRRRAEE
                     AQVDLLLPRPLGDKNLKERALRLLQPGGGQRKADAPAPEARLGTTLVLDETLEGLPRP
                     VADPTNATRSGAAPATRSSQTGEGAAGTEVTGTGTASGGAIGAAAAGSEATETTPQPL
                     AQAHLARAERESLLRENAALRNENRELRANIERLRRELNEARQVGRPGRR"
     gene            complement(680..1132)
                     /locus_tag="PKN52_11615"
     CDS             complement(680..1132)
                     /locus_tag="PKN52_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNR00646.1"
                     /translation="MESQSVGVEERGAGTNGARSASIRRCAAGSAIEILQEKWVLHIV
                     HALLNGSRCFNELGRDVGGCNPTTLAHRLARLESVGVIRKEVLHDDGHAAGLPRCSYE
                     LTEAGRQLQDVIDAIRVWAVAHLVVAGELEAGGTGGNDRFLEAGAGRP"
BASE COUNT          190 a          441 c          410 g          148 t
ORIGIN      
        1 gacttggacg gctattccct cgtctcgcgc gtcaagcggg tcgcccggct cgcgcggatc
       61 cccgtcatcc tcaccacgga ggccgacgcc ggcctcggcc tctcgcaacg cacccggcgg
      121 cgagccgagg aggcgcaagt agacctcctc ctaccgcggc cgctcggcga caagaacctc
      181 aaggagcgcg ccttacggct cctgcagccc ggtggcgggc aacggaaggc cgacgcgccg
      241 gcgccggagg cgcggctcgg cacgacgctc gtactggacg agacgctgga ggggttgccc
      301 cgccccgtcg cggatccgac gaacgccacc cggtccgggg cagcgccggc cacgcgctca
      361 tcccagacag gagagggggc ggcgggaacc gaggtcaccg ggacggggac ggcgagtggc
      421 ggcgcgatag gagctgcagc ggcaggaagc gaggcgaccg agaccacgcc gcagccgctg
      481 gcgcaggccc atttggcccg ggcggaacgc gagtccctcc tgcgcgagaa cgcggcgctg
      541 cgcaacgaga accgggaact gcgcgccaac atcgagcgtt tgaggcgcga gttgaacgag
      601 gccaggcaag taggcaggcc cggacggcgc tgagcgcggc cggccgaccg gtgggcggct
      661 cggcagcgga caccctgtct tacggtcgac cggcgccggc ctccaagaac cggtcgttgc
      721 cgcccgttcc ccctgcctcg agctcccccg ccacgacgag gtgggcgacg gcccacaccc
      781 ggatcgcgtc tatgacgtct tgcaactgcc tgccggcctc cgtgagttcg taactgcacc
      841 gcggcaggcc cgccgcatgt ccgtcgtcgt ggagcacttc cttgcggatc accccgacgc
      901 tctccagccg ggcgaggcga tgggccagcg tggtggggtt gcaaccgccg acgtccctgc
      961 ccagctcgtt gaagcagcgc gagccgttca gcagcgcgtg cacgatgtgc agcacccact
     1021 tctcctgcag gatctcgatg gcggaacccg ccgcacagcg cctgatggag gcgctcctgg
     1081 caccgttcgt gccggcacca cgctcctcga cgccgacgct ctgactctcc acgtccaaga
     1141 cgataccacc gccgcgccgc gcggggttta ccacggccta cacgacata
//
LOCUS       DAOQYE010000842         1188 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_157295_length_1188_cov_3.060900, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000842 DAOQYE010000000
VERSION     DAOQYE010000842.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1188
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_157295_length_1188_cov_3.060900"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="PKN52_11620"
     CDS             <1..283
                     /locus_tag="PKN52_11620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00647.1"
                     /translation="LGAAAYGDIPAAGVLPASLVPEVLALDDPGARTLRAAGVCVARA
                     VAGDEEGALREAAEAGIPYLLVAGVVRATGQHEAARQRELQGLLAGGLA"
     gene            280..930
                     /gene="hisG"
                     /locus_tag="PKN52_11625"
     CDS             280..930
                     /gene="hisG"
                     /locus_tag="PKN52_11625"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HNR00648.1"
                     /translation="MSSLEATPAITRAPSRLVLALPKGRVLPDALTTLRSAGLTLDLA
                     ADERALRHDGVDATVLVMRNADVPTYVELGVADAGVVGRDVLLEHGSALYAPVDLGFA
                     ACRLSLIRPVGAAGPVRRIASKYPRVTAEYLARIGSPAEVVELSGNVELACLSGLADA
                     VVDVVQTGATLRANGLEEVDVIAESSARFVVNRAALKLKADVLRPLIAALREGAGR"
BASE COUNT          158 a          409 c          451 g          170 t
ORIGIN      
        1 cctgggcgcg gccgcatatg gcgacatccc ggccgccggc gtgttgccgg cttccttggt
       61 gcccgaggtc ctggccctcg acgatccggg tgctaggact ctgcgcgccg ccggagtgtg
      121 cgtggcccgc gccgtggcgg gcgacgagga gggggcgctg cgcgaggccg cggaggccgg
      181 aatcccttac ctgctcgtgg caggggtcgt gcgtgcgacc ggccaacacg aggctgcgag
      241 gcagcgcgag cttcaggggc tgctggcggg tggcctggca tgagttcgct cgaagcgacg
      301 ccggcgatta cccgcgcgcc cagccggctc gtgctggccc tcccgaaggg tcgggtgttg
      361 ccagacgccc tcacgacctt gcgttccgcg gggctcaccc tcgacttggc cgccgacgag
      421 cgcgccctgc gccacgacgg ggtggacgcc accgtgctgg tgatgcgcaa cgcggacgtg
      481 ccgacgtacg tcgagctggg ggtcgccgac gccggcgttg tcgggcgcga cgtgctcctc
      541 gagcacggca gcgcgctcta cgcccccgtc gacctcgggt tcgccgcctg ccgactctcg
      601 ctcatccgcc cggtgggcgc cgccggcccg gtgcgacgca tcgcgagcaa gtacccgcgc
      661 gtgacggccg agtacctggc gcgcatcggc agccccgccg aggtcgtcga gctctccggc
      721 aacgtggagc ttgcctgcct ctccggcctg gcggacgccg tggtggacgt cgtacagacg
      781 ggggcgacgt tgcgcgccaa cgggctggag gaggtcgacg tcatcgccga gtcgagcgcg
      841 cggttcgtcg tcaaccgcgc ggcgttgaag ctcaaggccg acgtgctgcg accgttgata
      901 gccgcgttgc gggagggcgc gggccgctga gactcggcgg ccgctgaaag gcagcggtcg
      961 ccgaaaggca gcggtcgctg gagcccagcg gtcgctggag cccagcggtc gctgaggctc
     1021 agcgggcgcc ctagagccag cccctgcgcc tgaagtagac gatcatcccc gtggcgatgc
     1081 ccagcatcag gagccagagc gccgggtacg cccagcgcca gtggtactcg ggcatgtgct
     1141 cgaagttcat gccgtagata ccggccagga acgtcagcgg gatgaaga
//
LOCUS       DAOQYE010000843         1187 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_157609_length_1187_cov_2.234099, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000843 DAOQYE010000000
VERSION     DAOQYE010000843.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_157609_length_1187_cov_2.234099"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..907)
                     /locus_tag="PKN52_11630"
     CDS             complement(<1..907)
                     /locus_tag="PKN52_11630"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNR00649.1"
                     /translation="MAAEGGGNGLALYALRRAINAVPLILGVVVVNFVLIALAPGDPV
                     MSLIGEYPAPAEYLARVRQDFGLDKSVPERLWLYLVNVAKGDLGFSFANRQPVLDLLL
                     QRLGRTLVLMLSTVVVASTIGLFLGVLAARKPGSITDRGATGFALVGYAVPEFWLGQI
                     LILVFAVVLGWLPAGGFRSVRVEYTGLAAALDMLRHMLLPMAALSFRYMAITTRLTRA
                     SLLEVMSSQYIVAARGRGLSENTILWRHALRAAALPVVTVIGYNFGFIVAGSALVETV
                     FGWPGIGRLMYDSIYTRDYPVLLGIL"
     gene            complement(932..>1187)
                     /locus_tag="PKN52_11635"
     CDS             complement(932..>1187)
                     /locus_tag="PKN52_11635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00650.1"
                     /translation="TPFVNATGYSNPDIDAWLDQAGGLADQDARAEYYARINEVLAND
                     VPVMVMFDELASEASSGSLRGMRTLLDQRDGLEFLWFAQP"
BASE COUNT          216 a          387 c          431 g          153 t
ORIGIN      
        1 gcaagatacc gagcaacacg gggtagtcgc gcgtgtagat ggagtcgtac atgaggcggc
       61 ctatccctgg ccagccgaag acggtctcga cgagcgccga gcccgccacg atgaaaccga
      121 agttgtagcc gatgaccgtc acgacgggca gggcggccgc gcggagcgcg tgacgccaga
      181 ggatggtgtt ctccgacagc ccccgccccc gcgcagccac gatgtactgg gagctcatca
      241 cctctaggag cgacgcgcgc gtgaggcgcg tggtgatggc catgtacctg aacgagagcg
      301 cggccatggg caggagcatg tgcctgagca tgtcgagggc ggctgccaga cccgtgtact
      361 cgacccggac gctccggaag ccgccggcgg gcagccagcc gaggaccacg gcgaacacga
      421 ggatgaggat ctgcccgagc cagaactcgg gcacggcgta gccgacgagc gcgaagcccg
      481 tcgcgccgcg gtcggtgatc gagcctggct tgcgagccgc caacacgcca aggaagagcc
      541 cgatggtgga ggcgaccacc accgtgctca acatcagcac gagggtgcgg ccgagccgct
      601 ggaggagcag gtcgaggacg ggctggcggt tggcgaagga gaacccgagg tcccccttcg
      661 ccacgttcac caggtagagc cagagccgct cggggacgct cttgtcgagc ccgaagtcct
      721 gccgcacccg cgccaggtac tcggccggcg cggggtactc gccgatgagc gacatgacgg
      781 ggtcgccggg cgcgagggcg atgaggacga agttgacgac gaccacgccg aggatgagcg
      841 gcacggcgtt gatcgccctg cgcaatgcgt agagcgccag gccgttgccg ccgccctcag
      901 ccgccatgtc gcgccacctc ccggctttac gctagggctg ggcgaaccag aggaactcga
      961 gcccgtcgcg ctggtcgagg agcgtgcgca tgccgcgcag gctcccggag gacgcctcgg
     1021 aggccagctc gtcgaacatc accatcacgg gcacgtcgtt cgccagcacc tcgttgatcc
     1081 tggcgtagta ctcggcgcga gcgtcctgat cggcgagacc gcccgcctgg tcgagccacg
     1141 cgtcgatgtc ggggttggag tagcccgtgg cgttgacgaa cggggta
//
LOCUS       DAOQYE010000844         1187 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_157625_length_1187_cov_2.121025, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000844 DAOQYE010000000
VERSION     DAOQYE010000844.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_157625_length_1187_cov_2.121025"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            133..396
                     /locus_tag="PKN52_11640"
     CDS             133..396
                     /locus_tag="PKN52_11640"
                     /inference="COORDINATES: protein motif:HMM:NF025305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4212 domain-containing protein"
                     /protein_id="HNR00651.1"
                     /translation="MQLSAKHQEYWRKNLRLATILIAIWFVVTYVAAFFARSLTFNFF
                     GWPFSFWMAAQGALVVYLILIVYYARYMNRLDEEFGVAEGEDE"
     gene            393..>1187
                     /locus_tag="PKN52_11645"
     CDS             393..>1187
                     /locus_tag="PKN52_11645"
                     /inference="COORDINATES: protein motif:HMM:TIGR03648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VC_2705 family sodium/solute symporter"
                     /protein_id="HNR00652.1"
                     /translation="MSTAQSTQKAFAQQLKKVYGFYTGGFVTFVVILAILEQMGLPRQ
                     VIGYVFLIATVLLYAGIGIISRTNEAAEYYVAGRRVPAVFNGMAVGADWMSAASFIGM
                     AGTLYLAGFNGLAFVMGWTGGYVLTALFLAPYLRKFGQFTIPDFLGARYGGNIPRFIG
                     IVIAILCSFTYVVAQIYGVGIITTRLTGVAFEIGVFLGLAGILVCSFLGGMRAVTWTQ
                     VAQYIILIVAYMIPVVWLSVMQTGFPIPQLIYGQQLQKVTALEEKLI"
BASE COUNT          202 a          371 c          384 g          230 t
ORIGIN      
        1 acgccggcac cgaagatccg taccagccgg gcagccgcag gcggcgaccc ggcgacagcg
       61 attcatgaca cgggtggaac acccgacgag gagagcggag atgaggaaga gaaaccaaag
      121 ggagggccta gcatgcagct gagtgccaag caccaagagt actggcggaa gaacctgcgc
      181 ctcgcaacga tactgatcgc gatctggttc gtcgtcacct acgtcgccgc gttcttcgcg
      241 cggtcgctga ccttcaactt cttcggctgg ccattctcgt tctggatggc ggcgcagggg
      301 gcgttggtcg tctacctgat cctgatcgtc tactacgcac gctacatgaa ccggctcgac
      361 gaggagttcg gcgtcgcgga aggagaggac gaatgagtac cgctcagtca acccagaagg
      421 ccttcgcaca gcagctgaag aaggtctacg gcttctacac cgggggcttc gtcaccttcg
      481 tggtcatcct ggcgatcctc gagcagatgg ggctgccgcg ccaggtcatc ggctacgtgt
      541 tcctgatcgc caccgtgttg ctgtatgccg gcatcggcat catcagccgc accaacgagg
      601 ctgccgagta ctacgtggcc ggccggcgcg tgcccgcggt cttcaacggc atggcggtgg
      661 gcgccgactg gatgtcggcc gcttcgttca tcggcatggc gggcaccctc tacctggccg
      721 gcttcaacgg cctggcgttc gttatggggt ggaccggcgg ttacgtgctg accgcgctgt
      781 tcctggcgcc gtacctgcgc aagttcgggc agttcaccat tcccgacttc ctcggcgcgc
      841 gctacggcgg caacatcccg cgcttcatcg ggatcgtgat cgccatcctg tgctcgttca
      901 cgtacgtggt cgcgcagatc tacggggtgg gcatcatcac cacgcggctc accggcgtgg
      961 cgttcgagat cggcgtgttc ctcggtctcg ccggcatcct ggtgtgctca ttcctcggtg
     1021 gcatgcgcgc ggtgacgtgg acgcaggtgg cgcagtacat catcctgatc gtcgcctata
     1081 tgatcccggt cgtatggctg tcggtgatgc agaccggctt cccgatcccg cagctgatct
     1141 acggccagca gctgcagaag gtgacggcac tcgaggagaa gctgatc
//
LOCUS       DAOQYE010000845         1186 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_157727_length_1186_cov_3.492485, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000845 DAOQYE010000000
VERSION     DAOQYE010000845.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1186
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_157727_length_1186_cov_3.492485"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            308..859
                     /locus_tag="PKN52_11650"
     CDS             308..859
                     /locus_tag="PKN52_11650"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HNR00653.1"
                     /translation="MHHEGDEQGTLRHDDHRDELRSAVDGAREVLKDHGMRYSRPREV
                     ILTYLLEKDRHVSAESLYLDLKKRGEELSLSTVYLNLSALAGAGLVREFRGATGQALY
                     DSNVTPHYHVVCGETGEVRDVPAPMVNGVPLGRFLKEYVERETGWTVDEPRFSLTGKP
                     PADKARDRAGAWMANRSGDASDS"
     gene            876..>1186
                     /locus_tag="PKN52_11655"
     CDS             876..>1186
                     /locus_tag="PKN52_11655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00654.1"
                     /translation="MTDRDHVGDAGSERQGASFADDLVLRDLISHADLREVERIQRDV
                     WGLDDIEIVPSSQIRAALHAGGQAGGAFIGDALVGFSYGFLASPHGRLMEGPGLHSHM
                     AA"
BASE COUNT          204 a          394 c          424 g          164 t
ORIGIN      
        1 acccggtcac ggtgcgggac ggcgtcgttg cggtggtcga agggctgtcg gtgagcgcca
       61 cggtgcgaag caagatgaag gccacggagc aggggctctt ggaggagcgc gcggccgtgg
      121 aggcgctgct gtagcaggcg ggaggaccga ctggtcgtcc gggacgggcg tgtaggcggg
      181 gcgcgccagg gttattcttg gacgtcgagc gccgagatgc ctagcggcaa atcggagtcg
      241 ttccgacatg ctatcgttat cggagaggcg ggtcccacgg ggcccgacgg agaggcgacc
      301 cagctagatg caccacgagg gtgacgaaca aggcacgctg cggcatgacg accaccgcga
      361 tgagctccgg tccgcggtcg acggcgcccg ggaagtgctg aaggaccacg gcatgcgcta
      421 cagcaggccg cgggaagtca tcctcaccta cttgctggag aaggacaggc acgtcagcgc
      481 cgagagcctt tacctcgacc tgaagaagcg aggcgaggag ctcagcctct cgaccgtcta
      541 cctgaacctc tcggccctcg ccggcgcggg gctcgtgcgg gagttcaggg gcgccacggg
      601 gcaggccctg tacgacagca acgtgacccc ccactaccac gtcgtttgcg gcgagaccgg
      661 cgaggtgcgc gacgtccccg ctcccatggt gaacggcgtc ccgctcgggc gcttcctcaa
      721 ggagtacgtc gagcgcgaga cgggctggac cgtcgacgaa ccgcgcttca gcctcaccgg
      781 caagccaccc gccgacaagg cccgcgaccg ggcgggcgcc tggatggcaa accggtcggg
      841 cgacgcctcc gacagctgac cctcgaaaga ccttcgtgac agaccgcgat catgtggggg
      901 acgccggctc cgaacgccag ggcgcgtctt tcgcggacga cttggtcctc agagacctga
      961 tcagccatgc cgacctgcgc gaggtggagc gcatccagcg cgacgtctgg ggcctcgacg
     1021 acatcgagat cgtccccagc tcgcagatca gggccgcgct ccacgcgggt ggccaggccg
     1081 gtggcgcctt catcggcgac gccctggtgg gcttctccta cggcttccta gcctcgcccc
     1141 acggccgcct gatggaagga cccggcctcc actcgcacat ggcggc
//
LOCUS       DAOQYE010000846         1185 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_158026_length_1185_cov_2.401770, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000846 DAOQYE010000000
VERSION     DAOQYE010000846.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1185
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_158026_length_1185_cov_2.401770"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /locus_tag="PKN52_11660"
     CDS             <1..609
                     /locus_tag="PKN52_11660"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNR00655.1"
                     /translation="GFDFIRGDSLLLAIYCMVAITNLLDGAMFSILMPLWVMDRDLGP
                     EFLGLLAAVLSGASIASSLVAAAWGHRLPRRLVYLGGFFLAGAPRFLVLALGAGVPAI
                     LGTYLIAGLGSGFLNPIIGALQFERIPRNMLGTVRGLGTAISWAGQPFGGLVGGALAT
                     GFGLVKALLIGGTAYFLTTTLPGLLPQWAGMRGGKETPTARN"
     gene            complement(798..>1185)
                     /locus_tag="PKN52_11665"
     CDS             complement(798..>1185)
                     /locus_tag="PKN52_11665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00656.1"
                     /translation="LKLSVAAMALGGVAMAASPSVVVLVLGVMVTSLGNAPFNIAFMT
                     MVQRYSASGFLGRVRGVVDASGTAMFLAASSAAGLVVGWTTPRLMLGVSAAVMVIASL
                     LVTLELGPALRRTGLGKGEERGPDSR"
BASE COUNT          176 a          426 c          383 g          200 t
ORIGIN      
        1 gggttcgact tcatccgcgg cgattccctg ctcctcgcga tctactgcat ggtcgctatc
       61 accaacctgc tcgacggcgc catgttctcg atcctgatgc ccttgtgggt catggaccgc
      121 gacctcggac cggagttcct cggcctgctc gccgccgtgc tgagcggcgc ctccatcgcg
      181 tcgagcctcg tcgcggccgc gtggggtcac cgcctccccc gccggctcgt gtacctgggc
      241 gggttcttcc tcgccggcgc gccccgcttc ctggtgttgg cgctcggggc cggggtcccc
      301 gccatactcg gcacctacct gatcgccggg ttgggcagcg gcttcctgaa cccgatcatc
      361 ggcgcgttgc agttcgagcg catcccccgc aacatgctcg gcaccgtgcg tgggctcggg
      421 accgcgatct cgtgggccgg ccaaccgttc gggggcctgg tcggcggggc gctcgcgacg
      481 ggcttcggac tggtgaaggc gctgctgatc ggaggaacgg cctacttctt gaccaccacg
      541 ttgcccgggc tgttgccgca gtgggcgggg atgcggggcg gcaaggagac gccaacggcc
      601 cggaactgaa ctcggccggt accggcgaac gcggctcagc cggcctgcaa cccgagcatc
      661 gtagccaacg ccgtctgccc cgggtgcacg aggtgtaggt gtagctgacg acacggagct
      721 gctgacgccg ggtgcggccg atgcggtgag cgcgaagcgc gtcatcgctt ccgaaggacc
      781 tcagatgctc ggcatcgtca ccggctgtcg ggcccgcgct cctcgccctt gcccaggcct
      841 gtccggcgta acgctggtcc tagctccaac gtgaccaaca ggcttgctat caccatcaca
      901 gcagcggaaa cgccgagcat aagccgtggc gtggtccatc caacgaccag gccggccgcg
      961 cttgacgccg ccaggaacat cgccgttccg ctcgcgtcca cgacacccct aacgcgtccc
     1021 agaaagcctg aggctgagta gcgctgcacc atcgtcatga aggcgatatt gaacggggcg
     1081 ttgcccagcg acgtcaccat cactcccagc acgagcacca ctaccgacgg gctcgctgcc
     1141 atggcaacgc ctccaagggc catcgcggcc acgctcagct ttagg
//
LOCUS       DAOQYE010000847         1185 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_158132_length_1185_cov_1.612389, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000847 DAOQYE010000000
VERSION     DAOQYE010000847.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1185
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_158132_length_1185_cov_1.612389"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1002
                     /locus_tag="PKN52_11670"
     CDS             <1..1002
                     /locus_tag="PKN52_11670"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HNR00657.1"
                     /translation="GLRSAEHGGMIERLGFEADLHDYQPPPPQGLSGFLTIMRGCNHH
                     CTYCIVPDTRGPEVSRSAESIMREAEAMRAEGLSEVTLLGQNVNSYGIGQPDLPSFPE
                     LLRMVAALGFERVKFTTSHPMNFTSALIDVIATHPNVCNYIHLPMQSGSDRVLRRMAR
                     EYRIERYLDIVREIREKVAEVVISTDIIVGFPGETEEDFQATLAAAETAAFEQAYMFM
                     YSARPGTPSYKHFTDMPREVKVERLQRLIEAQKARSLAANDALVGKTLRVLVKDLGRD
                     SRYAVGHSDQNHTVLVPAESVTSMGVKQVTITGATPHALHGEVVGAAPRGIELVMAS"
BASE COUNT          202 a          420 c          391 g          172 t
ORIGIN      
        1 ggcctgcgct cggccgagca tggcggcatg atcgagcgcc tcggcttcga ggccgatctg
       61 cacgactacc agcccccgcc gcctcaggga ctgtccggct tcctcacgat catgcgaggc
      121 tgcaaccacc actgcacgta ctgcatcgtc cccgacacgc gcggccctga ggtctcgcgc
      181 tcggcggaga gcatcatgcg cgaggcggag gccatgcggg cggaggggct gtccgaggtc
      241 acgctcctcg gccagaacgt caactcctac ggcatcggcc agcccgacct accaagcttc
      301 cccgagctcc tccggatggt ggccgccctc ggcttcgagc gggtgaagtt cacgaccagc
      361 caccccatga acttcacgag cgcgttgatc gacgtcatcg ccacacaccc gaacgtctgc
      421 aactacatcc acctccccat gcagtcgggc tccgaccggg tgctgaggcg catggcgcgc
      481 gagtaccgca tcgagcggta ccttgacatc gtcagggaga tcagggagaa ggtggcggag
      541 gtcgtcatct cgacggatat catcgtcggc ttccccggcg agaccgagga ggactttcag
      601 gcgacgctcg cagcggcgga gacggccgcg ttcgagcagg cgtacatgtt catgtactcc
      661 gcgcgccccg gcaccccgag ctacaagcac ttcacggaca tgccgcggga agtgaaggtg
      721 gagcgcctgc agcgcctcat cgaggcgcag aaggcgcgca gcctggcggc caacgacgcg
      781 ctggtcggca agaccttgcg cgtgctcgtg aaggacctcg gccgcgactc gcgctacgcg
      841 gtaggccact ccgaccagaa ccatacggtc ctcgtgcccg ccgagagcgt cacgagcatg
      901 ggcgtgaagc aggtgacgat aacgggcgcc acgccgcacg cactgcatgg cgaggtcgtc
      961 ggcgcggcgc cgcgtggcat cgagctcgtg atggcgtcgt gagggcgtga cggaagctgc
     1021 accgcaccac ctcatgcgga cgcacctggc ggtcgtacac ccctccgaac ccacggtgct
     1081 cctggcggtg ggcggggacg acgcgccccg cctccccttc gtggaccgac cgctcgggga
     1141 ctcgcgcagc gcgctcgagt tcgctgacct gctcggaccc gagct
//
LOCUS       DAOQYE010000848         1181 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_158909_length_1181_cov_2.580817, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000848 DAOQYE010000000
VERSION     DAOQYE010000848.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1181
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_158909_length_1181_cov_2.580817"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..580)
                     /locus_tag="PKN52_11675"
     CDS             complement(<1..580)
                     /locus_tag="PKN52_11675"
                     /inference="COORDINATES: protein motif:HMM:NF016840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNR00658.1"
                     /translation="MAQTFQLGPTGGGDPRLETLLSELEIARRFTVSIVRGLTADELA
                     AKPQETRNSIGAILSHLAAAERLMQRITSLGQPFPAGTEDDRRVFAFEVEPLAGNALS
                     AYLAHLEETRARTRALFAEHDDAWLEEPRTFFGNPANYHYYWLHLLMDEARHQGQIIL
                     MRKYLMATADPWFDPYAGLAADAAGHDADDFGA"
     gene            684..>1181
                     /locus_tag="PKN52_11680"
     CDS             684..>1181
                     /locus_tag="PKN52_11680"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HNR00659.1"
                     /translation="MDKGPPMDKGPRRAAASSSVVSLVIALVLLVACAGQPVPPGGGN
                     VPEPFGRGINFANALEAPNEGEWGVTLQQGYAEAVKSAGFQTVRLPIKWSAHAAADAP
                     YAIDPAFLARVDEVVAWIVDRGMNVIVDFHHYDEIHVDPVGHAARWLGIWRQIAEHFQ
                     DAPASV"
BASE COUNT          185 a          387 c          407 g          202 t
ORIGIN      
        1 cggcgccgaa atcatccgcg tcatggcctg ccgcgtccgc cgccagcccg gcgtaagggt
       61 cgaaccaggg gtcggcggtg gccatcaggt acttgcgcat gaggatgatc tggccctgat
      121 gcctggcctc gtccatgagg aggtggagcc agtagtagtg gtagttggcc gggttcccga
      181 agaacgttct gggctcttcc agccaagcgt cgtcgtgctc cgcgaacaac gcacgcgtgc
      241 gcgcacgcgt ctcctccagg tgcgcgagat aggcggacag cgcgttgccg gccaacggct
      301 cgacctcgaa cgcgaacacg cggcggtcgt cctccgtacc cgcggggaac ggttgcccga
      361 gcgacgtgat gcgttgcatc aggcgctcgg cagccgccag gtggctgagg atcgcgccta
      421 tggagttcct tgtttcctgt ggcttcgccg ccagctcgtc ggcggtcaac ccgcggacga
      481 tgctgaccgt gaagcggcgc gcgatctcca gttcgctgag gagcgtctcg aggcgcggat
      541 cgcctccgcc ggtggggccg agctggaacg tttgagccat gggtgatggt agtacgtcgc
      601 gtcggcgtcg tttcggatcc tggtcgggac gaccgtgatg gattcaacag ccgcctctgc
      661 ggaacccgac cctatactcg cccatggaca agggaccgcc catggacaag ggaccacgtc
      721 gcgcagccgc ctcgtcatct gtcgtcagcc tcgtcatcgc cctcgtgctg ctcgtggctt
      781 gtgcaggtca gccggtgccg ccgggcggag ggaacgtgcc ggaaccattc ggccgcggca
      841 ttaacttcgc caacgccctt gaagcgccca acgagggcga gtggggcgtg accctgcagc
      901 agggctacgc cgaggccgtg aagagcgccg gcttccagac ggtcaggctc ccgatcaagt
      961 ggtcggcgca tgccgccgcg gacgcgccgt acgccatcga cccggctttc ctggcgcgcg
     1021 tcgacgaggt cgtagcctgg atcgtcgaca ggggtatgaa cgtcatcgtc gacttccacc
     1081 actacgacga gatccacgtc gacccggtcg gtcatgcggc gcggtggctc ggtatctggc
     1141 gccagatcgc agagcacttc caggacgcgc cggcgagcgt c
//
LOCUS       DAOQYE010000849         1181 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_158929_length_1181_cov_2.444050, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000849 DAOQYE010000000
VERSION     DAOQYE010000849.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1181
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_158929_length_1181_cov_2.444050"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="PKN52_11685"
     CDS             <1..478
                     /locus_tag="PKN52_11685"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNR00660.1"
                     /translation="TIPIIALVPVLLLLLGNAGVTVQTSLVPAALIGAYIAFFPVTVG
                     TVTGLRSVDPLSLDLMKSYAASPFQVFWKVRFPAAVPYLFVSLKLGAAAALVGALVAE
                     TESNNRLGLGYAIVGQVQAGNVSDVWILLLISAVLGIGLVALVGLLQRFTAPWERR"
     gene            463..>1181
                     /locus_tag="PKN52_11690"
     CDS             463..>1181
                     /locus_tag="PKN52_11690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00661.1"
                     /translation="MGASLMAERVPGAGSSGAAGSGGPAGSAGPAGSGEPAAPTGPTA
                     STASTGAALAWRPHLPTLLMLLVAVAGLFGPFSVGGGGLLLGGSGLAVGCLVLLAAAL
                     APTGWGGPWAVPLVTPLAVSSAFLALLAQRGLAGPAGWGYWLTLLGSPAAATTAAGRV
                     SVIELESDAPRWQHQVQRLAPAATVAFLVLLAWEGLVVGSRVPKGIFPRVSDIWLAFV
                     GTWRVLLADAYLTFVKEVLFG"
BASE COUNT          106 a          454 c          403 g          218 t
ORIGIN      
        1 gacgatcccg atcatcgcgc tcgtcccggt actcctgctc ctcctcggca acgccggcgt
       61 gaccgtccag acgagcctgg tccctgccgc gctcatcggg gcctacatag cgttcttccc
      121 cgtgaccgtc ggcaccgtga caggcctgcg ctccgtcgac ccgctgtcgc tcgacctcat
      181 gaagagctac gcggcctcac cgttccaggt cttctggaag gtgcgtttcc cagccgccgt
      241 gccctacctg ttcgtgagcc tcaagctcgg ggcggccgcg gcgctcgtcg gcgcgctggt
      301 cgccgagacc gagtcgaaca accgcttggg cctcggctac gccatcgtcg gtcaggtgca
      361 agccggcaac gtctcggacg tctggatact gctgctcatc tcggccgtgc tcggtatcgg
      421 cctcgtggcc ctggtagggc tgctccagcg cttcactgcg ccgtgggagc gtcgctgatg
      481 gcggagcgcg ttccaggcgc tggctcgagc ggagccgctg gctctggcgg gcccgccggc
      541 tctgccggtc ccgctggctc tggcgagcct gccgccccga ccggcccgac cgcctcgacc
      601 gcctcgaccg gcgccgcgct cgcctggcgc ccgcacctgc ccacgctgct gatgctcctg
      661 gtcgccgtcg ccggcctctt cggaccgttc tcggtcggtg gcggcggtct gctgcttggc
      721 ggcagcggcc tggccgtcgg ctgccttgtg ctcctcgcgg cggcactggc gcctaccggc
      781 tggggcggcc catgggccgt gcccctggtc acgccgctgg ccgtctcgtc cgccttcctg
      841 gccttgctcg cgcagcgcgg cctcgcgggt ccggccggtt ggggctactg gctgacgctc
      901 ctcggttcgc cggccgccgc caccacggcc gccgggcggg tgagcgtcat cgagctcgag
      961 agcgacgcgc cccgctggca gcatcaggtg cagcggctcg cgcccgccgc cacggtggcg
     1021 ttcctcgtcc tcctggcctg ggagggcctg gtcgtcggct cgcgcgtgcc gaaggggatc
     1081 ttcccgcgcg tctccgacat ctggctcgcc ttcgtcggga cctggcgcgt gctgttggcc
     1141 gacgcctacc tcacgttcgt gaaggaggtc ctgttcggct t
//
LOCUS       DAOQYE010000850         1181 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_159007_length_1181_cov_1.885435, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000850 DAOQYE010000000
VERSION     DAOQYE010000850.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1181
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_159007_length_1181_cov_1.885435"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /locus_tag="PKN52_11695"
     CDS             complement(<1..409)
                     /locus_tag="PKN52_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HNR00662.1"
                     /translation="MCGIVGYVGGREAAGVILDGLGRLEYRGYDSAGLAVKGLGADGQ
                     LRAVKRAGKLSVLRDAVAAGLPAGCVGLGHTRWATHGRPNDVNAHPHLSEDGRLAVIH
                     NGIIENYVELREELAARGHVFASETDTEVLVHLM"
     gene            complement(603..>922)
                     /locus_tag="PKN52_11700"
     CDS             complement(603..>922)
                     /locus_tag="PKN52_11700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00663.1"
                     /translation="VDEMAVDIENGGAVLGLSLMFALVTSLPIERSPVNEREIFYPPR
                     TAPIGLAVTFAQSPLVLEGRSILLHPLLPAPTPAEGLKNVTWRAEGLLHRWLVVGEPW
                     NAP"
     assembly_gap    923..932
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<933..1157)
                     /locus_tag="PKN52_11705"
     CDS             complement(<933..1157)
                     /locus_tag="PKN52_11705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00664.1"
                     /translation="MTALGAKRRLVGLVVGVGGALLLRPLIIIGSRDLWIALAAAFCG
                     TLILALIGQRLAPPNRRQHWGTAAAGGVGGA"
BASE COUNT          187 a          407 c          407 g          170 t
ORIGIN      
        1 ccatgaggtg cactagcacc tcggtgtcgg tctcggaggc gaacacgtgg ccgcgggcgg
       61 cgagctcctc gcgcagttcg acgtagttct cgatgatgcc gttgtggatg acggccaggc
      121 ggccgtcctc agagaggtgc gggtgggcgt tgacgtcgtt ggggcggccg tgcgtggccc
      181 agcgcgtgtg gccgagcccg acgcagccgg cggggaggcc cgccgccacg gcgtcgcgca
      241 gcacgctgag cttgccggcg cgcttgactg cgcgcaactg accgtctgcc cctaggccct
      301 taacggccag gccggcggaa tcgtaacccc ggtactcgag acggccgaga ccatcgagga
      361 tgacccctgc ggcctcacgg ccaccaacgt aaccaactat tccgcacatg gtcccattct
      421 cccctcatgg cattacgccg ttcttacgtg ggagcaggtt acggccgcgc gcagcccagg
      481 gtcgtcgcaa ccgccacgtc gatgacgcgt catcgacacg tcatcgatgc atcatcgacg
      541 cgtcgatgac gcatcgcggg gccccctcgc cgacctcttc tcgcgagggc cctcgccgac
      601 ctctaaggag cgttccacgg ctcgcccacg acgagccagc ggtggagcag gccctcagcc
      661 cgccaggtga cgttcttcag gccctcggcc ggcgtcggcg ccgggaggag cgggtgcagg
      721 aggatggagc gaccctcgag gacgaggggc gactgcgcga acgtgacggc cagcccgatc
      781 ggggccgtgc gcggggggta gaagatctcc cgctcgttga cggggctccg ctcgatgggc
      841 agcgacgtca cgagcgcgaa catcaggctc aggccgagga cggcaccgcc gttctctata
      901 tcgaccgcca tctcgtccac gannnnnnnn nnggcaccgc ctaccccgcc cgccgcggcg
      961 gtgccccagt gttgcctgcg gttgggcggc gcgaggcgtt ggccgatcag ggcgaggatg
     1021 agggtgccgc agaaggctgc ggcgagcgct atccagaggt cacgcgagcc gatgatgatg
     1081 agcggcctca ggagcagcgc tcccccgacg ccgaccacca gcccgaccag gcgccgtttg
     1141 gcccccaacg ccgtcatgat cacgatcatg agcactaggg c
//
LOCUS       DAOQYE010000851         1180 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_159244_length_1180_cov_1.948444, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000851 DAOQYE010000000
VERSION     DAOQYE010000851.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1180
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_159244_length_1180_cov_1.948444"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="PKN52_11710"
     CDS             <1..291
                     /locus_tag="PKN52_11710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00665.1"
                     /translation="YNLTRVAALQGVRVLNVHQLASAVKASYLPGERLALSIVRQGRE
                     SGQGLAYLEDGTMVVVEGAGDRVGTTIDVVVTSSLQTNMGRMLFARPRDAVP"
     gene            441..516
                     /locus_tag="PKN52_11715"
     tRNA            441..516
                     /locus_tag="PKN52_11715"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:474..476,aa:Ala,seq:tgc)
     gene            665..>1180
                     /locus_tag="PKN52_11720"
     CDS             665..>1180
                     /locus_tag="PKN52_11720"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNR00666.1"
                     /translation="MLQASIFRDLSASDISELLPGLIERSFVRGEVVWLAGEPADVLV
                     VLAEGQLKVHRINSDGREVIVDVVTAVATTGEVGLFHPAGSRWLNLSAMTATRCLMIR
                     RAALLAFLSRHPAALQRMLELLASAAVLATNSLSSVAFDDIGHRVAGRLLEMADTQAE
                     QTADGLRLTPRL"
BASE COUNT          180 a          401 c          401 g          198 t
ORIGIN      
        1 tacaacctca cccgcgtggc ggcgctgcag ggggtgaggg tgctgaacgt ccaccagctc
       61 gccagcgcag tcaaggcgtc ctacctgcca ggcgagcggc tggcgctcag catcgtcagg
      121 cagggacgcg agtcagggca gggcctcgcc tacctggagg acggcaccat ggtcgtcgtc
      181 gagggggccg gcgaccgcgt cggcaccacc atcgacgtcg tggtcacctc cagcctgcag
      241 acgaacatgg ggcgcatgct cttcgcccgg ccccgtgatg ccgtgcccta gcgcgcgccg
      301 tcggcctccg ccccgtttcg ccggcgaccc gccgaacccg cgccgtaagc ctccgcacgc
      361 gccttgacag gccaccccat gcccgacata ctctcaaggc gcccggcgag cttccttcag
      421 ggaagcccgc gggcggacgc ggggccttag ctcagctggg agagcactcg ctttgcaagc
      481 gagatgtcgc cggttcgagt ccggcaggct ccaccaaagg agtgggtgac tggaactttg
      541 tatacaaagt tccagtcacc catttcgctt acctggcacc gggcaacgcg gtcgtcgatg
      601 gtcatcctcg tggtgatgcc gcgcgcgttc gtgccaggct gaggcgaagg acgcggctgc
      661 gatcttgctc caggcatcga tcttccgtga cctctcggcc tcggacatca gcgagttgct
      721 ccccggcctg atcgaaagat ccttcgtccg tggcgaggtg gtctggttgg caggcgaacc
      781 ggccgacgtg ctcgtcgtgc tcgcggaagg gcagttgaag gtgcaccgga tcaactcgga
      841 cggccgcgag gtgatcgtgg acgtggtcac cgccgtcgcc acgacgggcg aggtcgggct
      901 cttccatccg gccgggtcga ggtggctgaa cctgagcgcg atgacggcca cgcgctgcct
      961 catgatccgt cgcgcggcgc tgctggcctt cttgtcgcgt catccggcgg cgctgcagcg
     1021 catgctcgag ctcctggcct cggccgcggt gctcgctacg aactcgctca gcagcgtggc
     1081 cttcgacgac atcggtcacc gggtggccgg tcgtctgttg gagatggcgg atacacaagc
     1141 cgaacagacc gcggacgggc taaggctgac gccgcgactc
//
LOCUS       DAOQYE010000852         1176 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_160047_length_1176_cov_2.510259, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000852 DAOQYE010000000
VERSION     DAOQYE010000852.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1176
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_160047_length_1176_cov_2.510259"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..515
                     /locus_tag="PKN52_11725"
     CDS             <1..515
                     /locus_tag="PKN52_11725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00667.1"
                     /translation="RRGAKSSQPRYYLDLHRERRKQAAGETAYTPAVSLVAGLDVALG
                     LLLAEGVDAVWRRRATLNAGLLAAGVALGMRPLAQRPSPAVAALLVPEGVGAPAVVAA
                     AMARGAVIAGGQDELKPVLIRPSLLGHADRYDALGAAQLIEDALADVWAAAPRGVAAP
                     AALRAMDGVE"
     gene            512..>1176
                     /locus_tag="PKN52_11730"
     CDS             512..>1176
                     /locus_tag="PKN52_11730"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNR00668.1"
                     /translation="MSAAAAADAAADAETLDAPKSGPAGGELVLVRHAPTTPVPGAAA
                     ATWRLAPGAMELTAALGMALVGVRLAAPPGGSAGPVDAVVSSHEPKALATARTLSLVL
                     GVPTTTGTDLEEHHRDDVPFMPDVEFLRTVKRFFARPDVLVFGSETAREAEGRFRKGV
                     IDALASRPRKRLAVVTHGTVMALALAQANGLEPFALWQSLKLPEAIVVRRDLTIVERL
                     SLEV"
BASE COUNT          143 a          438 c          444 g          151 t
ORIGIN      
        1 ggcggcgcgg cgcgaagagc agccagccac gctactacct cgaccttcac cgcgaacggc
       61 gcaagcaggc ggcgggggag acggcgtaca cgccggccgt gtcgctcgtg gccggcttgg
      121 acgtcgccct cggcctgctg ctcgccgagg gcgtggacgc cgtgtggcgc cgccgcgcga
      181 ccctcaacgc cggcctcctg gcagccgggg tcgcgctcgg catgcggccg ctcgcgcagc
      241 ggccaagccc ggccgtggcg gcgctgctag tgccggaagg cgtcggcgct ccggccgtcg
      301 tggcggcggc catggcgcgc ggcgccgtca tcgccggtgg ccaggacgaa ctcaagccag
      361 tgctgatcag gccgtcgctg ctcggccacg cggaccgtta cgacgcgctc ggcgccgccc
      421 agctcatcga ggacgcgctg gccgacgtct gggcggccgc tccgcgcggc gtggccgcgc
      481 ccgccgccct gcgcgccatg gacggggtcg agtgagcgcc gccgctgctg cggacgccgc
      541 tgcagacgcc gagacgttgg acgccccgaa gtcgggtccg gccggtggcg agctcgtgct
      601 cgtcaggcac gcgcccacga cccccgtgcc cggcgcggcg gccgcgacct ggcggctggc
      661 gccgggggcg atggagttga cggcggcgct cggcatggct ctggtcggag tgcgcctggc
      721 cgcgccgcct ggcggttccg ccggacccgt cgatgcggtc gtctcgagcc acgagccgaa
      781 ggcgctcgcg acggcgcgca ccctctcgct cgtgctcggc gtgcccacca cgaccggcac
      841 ggacctcgag gagcaccacc gggacgacgt ccccttcatg cccgacgtcg agttcctgcg
      901 cacagtcaag cgcttcttcg cgcgcccaga cgtactcgtg ttcggcagcg agacggcgcg
      961 ggaggcggaa gggcgcttcc gcaaaggagt tatcgacgcg ctggcctccc ggcccaggaa
     1021 gcgcctggcg gtagtgacgc acggcacggt catggcgctg gccctcgccc aagccaacgg
     1081 gctcgagccg ttcgcgctct ggcagtccct gaagctaccg gaggcgatcg tcgtgcggcg
     1141 cgacctgacc atcgtggagc ggctgtcgct ggaagt
//
LOCUS       DAOQYE010000853         1175 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_160217_length_1175_cov_3.606250, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000853 DAOQYE010000000
VERSION     DAOQYE010000853.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1175
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_160217_length_1175_cov_3.606250"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="PKN52_11735"
     CDS             <1..277
                     /locus_tag="PKN52_11735"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179049.1"
                     /note="catalyzes the formation of prolyl-tRNA(Pro) from
                     proline and tRNA(Pro); Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HNR00669.1"
                     /translation="FHDALYQRAVEFRQQRIYHAETYEELKEKVELGFVYATHCGDPE
                     SEKAIQEETKATVRCIPLEGPSAEGTVCVHTGRPSGYARKVIFAKAY"
     gene            277..696
                     /locus_tag="PKN52_11740"
     CDS             277..696
                     /locus_tag="PKN52_11740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00670.1"
                     /translation="MADGAREGARPAPRKVVDNDLYERIVKAQAVILQRALRSGVKVS
                     FGEGRRATDVAMVLLKGDRDAVLALPGQLEDELEFDLHVLRSTFSRESFELLLVFMEP
                     IDDQALRSPERRGHLRRRFQQLDADEEGTRLLDKDPE"
     gene            complement(730..>1175)
                     /locus_tag="PKN52_11745"
     CDS             complement(730..>1175)
                     /locus_tag="PKN52_11745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00671.1"
                     /translation="PKAQGYTPLTLKRAGESAGHVIYGSPNGGPSVPNDHYRSVVRTG
                     YEYVYEHTGNTPTNFSIDLNDVVKGDRMIVSVPYSGTPEPFIYRDWWIDARSLIPSYM
                     SLMALRSATDTGYFLDAPSRRLYLMLVQQGDRDYARLTVCQKAGC"
BASE COUNT          201 a          379 c          419 g          176 t
ORIGIN      
        1 cttccacgac gcgctgtacc agcgcgccgt ggagttcagg cagcagcgca tctaccacgc
       61 cgagacgtac gaggagctca aggagaaggt cgaactgggc ttcgtgtacg ccacgcactg
      121 cggcgacccg gagagcgaga aggccattca agaggagacg aaggcgaccg tgcggtgcat
      181 cccgctggaa ggtccgagcg ccgagggcac ggtctgcgtg cacacgggcc gcccgagcgg
      241 ctacgcgcgc aaggtcatct tcgccaaggc gtactgatgg ccgacggcgc gcgcgagggc
      301 gccaggccag cgcccaggaa ggtcgtggac aacgacctct acgagcgcat agtcaaggcg
      361 caggccgtca tcctgcagag ggccctgcgc agcggcgtga aggtcagctt cggcgagggg
      421 cggcgcgcca cggacgtggc catggtcctc ctgaagggcg atcgcgacgc cgtgctggcg
      481 ctgccggggc agctcgagga cgagttggag ttcgacctgc acgtcctgcg cagtacgttc
      541 tcgcgcgaga gcttcgagct cctgctcgtg ttcatggagc ccatcgacga ccaggccctg
      601 cgcagccccg agcgccgcgg ccacctgcgg cgacggttcc aacagctcga cgccgacgag
      661 gagggaaccc ggctcctcga caaggacccc gaatagagac ggggcgcgct tgggagcgcg
      721 ccccgatcgc tagcagcccg ccttctggca caccgtgagc cgcgcgtaat cccggtcgcc
      781 ctgttggacg agcatcaggt agaggcgccg ggacggtgcg tcgaggaagt agccggtgtc
      841 ggtcgcgctg cgcagcgcca tcaggctcat gtacgaaggg atgaggctgc gcgcgtctat
      901 ccaccagtcc cggtagatga agggctccgg cgtgccggag taagggacgc tgacgatcat
      961 gcggtcgccc ttcaccacgt cgttcagatc gatgctgaag ttcgtcgggg tgttgcccgt
     1021 gtgctcgtag acgtactcgt agccggtgcg caccacgctc cggtagtgat cgttcggcac
     1081 ggacggcccg ccgttcggac tgccgtagat gacgtggccc gccgactcgc cggcgcgctt
     1141 gagcgtcaac ggtgtatagc cctgtgcctt ggggc
//
LOCUS       DAOQYE010000854         1175 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_160246_length_1175_cov_2.875000, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000854 DAOQYE010000000
VERSION     DAOQYE010000854.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1175
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_160246_length_1175_cov_2.875000"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1175)
                     /locus_tag="PKN52_11750"
     CDS             complement(<1..>1175)
                     /locus_tag="PKN52_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HNR00672.1"
                     /translation="PERMVLDVVPVLPPELRPLVPLDGGRFATSDLNDLYRRVINRNN
                     RLKKLMQQGAPEMIVRNEKRMLQEAVDALIDNGRRGSAVVHPGSDRPLRSLTDLLGGK
                     QGRFRQNLLGKRVDYSGRSVIVVGPQLQLHQCGVPKRMALELFKPFLFKKLEERGIVS
                     NIKSARKMLERYRDARDEVWDALEEVIKDRVVLLNRAPTLHRLGIQAFEPVLVEGQAI
                     QLHPLVCEAFNADFDGDQMAIHVPLSVYAQSEARLQMLSSHNLLSPAHGNPNVQATRD
                     IILGLYVLTQLHRGHRGIGAEFKDHEAALAAYERGEVDLNSDITVAGKPTSVGRLMYH
                     FGSVDEALLAVAEHRIDMQDVVTARVDGEFVETSPGRLLFARMVKETLEAGGEVPRD"
BASE COUNT          171 a          361 c          443 g          200 t
ORIGIN      
        1 gtcgcggggc acctcgccgc cggcctccag ggtctccttg accatcctgg cgaacaggag
       61 ccgcccgggg ctcgtctcga cgaactcgcc gtcgacgcgg gccgtgacga cgtcctgcat
      121 gtcgatgcgg tgctcggcga cggccaggag cgcctcgtcg acgctgccga agtggtacat
      181 gagacggccg acgctcgtag gcttgccggc gaccgtgatg tcggagttga ggtcgacctc
      241 accacgctcg taagccgcaa gcgccgcctc gtggtccttg aactcggcgc cgatgccgcg
      301 gtggcctcgg tggagctgcg tgaggacgta gaggccgagg atgatgtcgc gcgtcgcctg
      361 gacgttcggg ttgccgtgcg ccgggctcag gaggttgtgg ctcgagagca tctggagccg
      421 cgcctcggac tgcgcgtaga cggacagcgg cacgtggatg gccatctggt cgccgtcgaa
      481 gtccgcgttg aacgcctcgc acaccagcgg gtggagctgg atggcctgac cttctacgag
      541 cacgggctcg aacgcttgga tgccgaggcg gtggagcgtc ggggcgcggt tgaggaggac
      601 gacgcggtcc ttgatgacct cttcgagggc gtcccagacc tcgtcgcggg cgtcgcggta
      661 gcgctccagc atcttgcggg cgctcttgat gttcgagacg atgccccgct cctcgagctt
      721 cttgaagagg aacggcttga agagctcgag cgccatgcgc ttgggcacgc cgcactggtg
      781 gagttggagc tgcgggccga cgacgatgac ggagcgcccg gagtagtcga cgcgcttgcc
      841 gagcaggttc tgcctgaacc ggccctgctt gccgccgagc aggtcggtga gcgagcgcag
      901 ggggcggtcg gaaccggggt gcacgacggc gctgccgcgg cgaccgttgt cgatcagggc
      961 gtcgacggcc tcttgaagca tgcgcttctc gttgcgcacg atcatctcgg gggcgccctg
     1021 ctgcatgagc ttcttgaggc ggttgttgcg gttgatgacg cggcggtaga ggtcgttcag
     1081 gtcggaggtg gcgaaacggc cgccatcgag gggcaccagg gggcgcaact cagggggcag
     1141 cacgggcacc acgtccagca ccatcctctc gggct
//
LOCUS       DAOQYE010000855         1171 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_161109_length_1171_cov_2.973118, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000855 DAOQYE010000000
VERSION     DAOQYE010000855.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1171
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_161109_length_1171_cov_2.973118"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1090)
                     /gene="speA"
                     /locus_tag="PKN52_11755"
     CDS             complement(<1..1090)
                     /gene="speA"
                     /locus_tag="PKN52_11755"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HNR00673.1"
                     /translation="MSRPTLDPGFSLTDADELYSIRAWSSGYFGVGKDGQLTVSLKGS
                     PAVPLADIVGGLTAAGHALPLVLRFPDILEDRMDRINEAFAAAIAEAGYQNRYQGVFP
                     IKVNQRRMVVETIASYGAKYRTGLEAGSKAELALCLAHETHDDALICCNGFKDDDFIR
                     LALWGRKLGRNVIITLEKAGELERVLRISKEVGVEPLLGVRFKLHARGSGQWEASGGD
                     DAKFGLTASELITVAQRVQAEGLGHSLVLLHCHVGSQLTDIRRIRAAVREAAQAYVEL
                     AEMGVGVKYLDVGGGLAVDYDGSKTTYYTSANYGLREYADTVVYTIMESCQEWEVAHP
                     VIVTESGRALTAHHSVVIVPVVDAIGPTR"
BASE COUNT          160 a          412 c          403 g          196 t
ORIGIN      
        1 cgcgggtggg cccgatcgcg tcgacgaccg gcacgatcac gacggagtgg tgcgccgtga
       61 gggccctccc ggactccgtg acgatgaccg ggtgcgcgac ctcccactcc tggcacgact
      121 ccatgatggt gtagacgacc gtgtcggcgt actcgcgcag gccgtagttg gccgaggtgt
      181 agtaggtcgt cttcgacccg tcgtagtcca ccgccaggcc gcctcccacg tcgaggtact
      241 tgaccccgac gcccatctcg gcgagctcga cgtaggcctg ggccgcctcg cgcacggcgg
      301 cgcggatgcg ccggatgtcg gtcagctggc taccgacgtg gcaatgcagg agcacgaggc
      361 tatggccgag accctccgcc tgcacgcgct gcgcgacggt gatgagctcc gaggccgtga
      421 ggccgaactt ggcgtcgtcg ccccccgacg cctcccactg gccgctgccg cgcgcatgca
      481 gcttgaagcg cacgccgagc agcggctcga cgccgacctc cttgctgatg cgcagaacgc
      541 gctccagctc gccggccttc tccagcgtga tgatgacgtt gcgcccgagc ttgcggcccc
      601 acagggccag ccggatgaag tcgtcgtcct tgaagccgtt gcagcagatg agcgcgtcgt
      661 cgtgcgtctc gtgcgccagg cagagggcga gctccgcctt gctgcccgcc tcgaggcccg
      721 tgcggtactt ggcgccgtac gacgcgatgg tctcgaccac catcctgcgc tggttgacct
      781 tgatggggaa cacgccctgg taacggttct gatacccggc ctccgctatc gcggccgcga
      841 acgcctcgtt gatgcggtcc atgcggtcct cgaggatgtc cgggaagcgc agcaccagcg
      901 gcagcgcgtg cccggccgcc gtgaggccgc ccacgatgtc ggcgagtggc acggcgggcg
      961 aacccttcag cgacaccgtc agctggccgt ccttccccac cccgaagtag ccgctcgacc
     1021 acgccctgat gctgtagagc tcgtcggcgt cggtgaggct gaaacctggg tcgagggtgg
     1081 gtctgctcac ttctggtcct ccacttggcg ggtgctcagc cgtgctgctt tgggctactc
     1141 ggtcggggcg cggcgcggcc tggcgcgcgg t
//
LOCUS       DAOQYE010000856         1171 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_161155_length_1171_cov_2.457885, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000856 DAOQYE010000000
VERSION     DAOQYE010000856.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1171
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_161155_length_1171_cov_2.457885"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="PKN52_11760"
     CDS             <1..256
                     /locus_tag="PKN52_11760"
                     /inference="COORDINATES: protein motif:HMM:NF021378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HNR00674.1"
                     /translation="LGPGHSRYGHDVPGHDQAWQTARERLANGEVTPQEFETLKRGLA
                     HYGEGRPQFDNALNLARMRFAKGEISLEDLEAIKRALTQA"
     gene            446..1126
                     /locus_tag="PKN52_11765"
     CDS             446..1126
                     /locus_tag="PKN52_11765"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNR00675.1"
                     /translation="MATGTVLIIEDERNLVGILTDYLKREGYRVESAFDGARGLELWR
                     AARPDLVLLDLMLPGVDGLEVARRIRGESAVPIIMLTARDAEVDKLVGLGIGADDYVV
                     KPYSPREVVARVKAVLRRAGGAMPTQDRFTVGELSIDLGAYVVECGGGRVDLTASELR
                     LLAELARDPGRVKRRAELLASVGAGESLADERTVDAHVKNLRRKLGACGEMIETVRGV
                     GYRLRDQR"
BASE COUNT          201 a          380 c          423 g          167 t
ORIGIN      
        1 gctcgggccg gggcacagta ggtatgggca cgacgtacct ggacacgacc aagcttggca
       61 gaccgcccgc gagcgtcttg ccaacggcga ggtgacccca caggagttcg agaccctgaa
      121 gcgcgggctc gcccactacg gcgaagggcg gccgcagttc gacaatgcgc tgaacctggc
      181 ccgcatgcgc ttcgcgaaag gcgagataag cctcgaggac ctcgaggcga tcaagcgggc
      241 gttgacacag gcctagtcat catcggaacc gggccgggcg ttggcgcaag ctagacggcc
      301 cgcaaccgga tcgagcatct acgcaccgtc aaggcgtgga tgctcgatcc gcctatatca
      361 cccatacctt ggcatgaagc cggcgccgac tcgataacgg ccggcacgtc cggtcgcacc
      421 gccaaggacg gctagagtgg agaagatggc gacgggcacg gtcctcatca tcgaagacga
      481 gcggaacctg gtaggcatcc tcacggacta cctcaagcgt gagggttacc gggtggagag
      541 cgcgttcgac ggcgcgcgtg ggctcgagtt gtggcgcgcc gcgcgtccgg acctggtcct
      601 cctcgacctc atgcttccgg gcgtggacgg gctcgaggtc gcccggcgca tccggggcga
      661 gtccgccgtg ccgatcatca tgctcacggc ccgcgatgcc gaggtcgaca agctcgtggg
      721 cttgggcatc ggcgcggacg attacgtggt caagccgtac agcccgcgcg aggtggtcgc
      781 gcgcgtcaag gccgtgctgc gccgcgcggg tggcgccatg ccgactcagg atcggttcac
      841 ggtaggcgag ctgagcatcg acctcggcgc ctacgtcgtc gagtgcggcg gtgggcgggt
      901 cgacctcacg gccagcgagc tccgcctgct ggccgaactc gcgcgtgacc cggggcgcgt
      961 taagcgccgg gcggagctcc ttgcgagcgt gggcgcggga gagagcttgg cggacgagcg
     1021 caccgtcgac gcccacgtga agaacctccg tcgcaagctc ggagcgtgcg gcgagatgat
     1081 cgagaccgtc aggggcgtcg gttaccgcct gcgagaccag cggtgagccg acaccgtccc
     1141 cctggtcccc catggggtga acggcacgtc g
//
LOCUS       DAOQYE010000857         1171 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_161232_length_1171_cov_1.954301, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000857 DAOQYE010000000
VERSION     DAOQYE010000857.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1171
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_161232_length_1171_cov_1.954301"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="PKN52_11770"
     CDS             complement(<1..484)
                     /locus_tag="PKN52_11770"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNR00676.1"
                     /translation="MSADLTVTSGEYVAITGPSGSGKSTLLSLLGLLDRSSAGSYAID
                     GMEAAGLGDRAASALRNEVIGFVFQGFHLIPHLNAWRNVALPLSYTPRRASISKTERR
                     DRALAALDSVGMLQHAEHLPRELSGGQEQRVAIARALVTKPQVILADEPTGNLDSKAR
                     D"
     gene            complement(608..>1171)
                     /locus_tag="PKN52_11775"
     CDS             complement(608..>1171)
                     /locus_tag="PKN52_11775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00677.1"
                     /translation="VLCADNEHILGWARSQRQWRPTAAALGRFVEACAKGEFTGLSAD
                     YQLPAEFLSKYDLTGTWFAVLILDAFSQPVAVSKTPGAARTVLEAANAPIPLFLAAGE
                     ALTEDYETVVPAEEHRAALAGLGVEVDAGLSDALPDWVYDFATHPATTLVIFDDSGRA
                     VAHFAATPSNPTEAQNLQQWMTLHGLF"
BASE COUNT          202 a          356 c          399 g          214 t
ORIGIN      
        1 cgtcgcgtgc cttcgaatct aggttgccgg tgggttcgtc ggcgaggatc acctgtggct
       61 tggtgacgag cgcgcgtgct atggcaacgc gctgctcctg tccgccggac agttcgcgcg
      121 gtagatgttc ggcgtgctgg agcatgccta cagagtccag cgctgccagc gccctgtcgc
      181 ggcgttcagt cttgctgatg ctggcgcgcc tcggtgtgta agacagcggc aaagccacgt
      241 tgcgccaagc gttgaggtgc gggatgaggt ggaacccttg gaagacgaat ccgatcacct
      301 cgttgcgtaa ggcggatgcc gcgcggtcgc cgagaccggc tgcctccatt ccatctatgg
      361 cgtagcttcc agcgctagag cggtcgagca ggccaagcaa actcaacaag gtggacttac
      421 ccgaacccga cggcccagtg atagctacgt actcgccact tgttacggtc aggtcggcgc
      481 tcatcagcgc cctgacctca cgggcgccct tcctatgtac gcgcgtcagg ccctgcgctt
      541 gaaggagcgg tcgatgaatc accggcccgc cggtcgtttg agaaggtgtc acggccacac
      601 ctcggttcta aaagagccca tggagggtca tccactgctg aaggttttgc gcctcggtcg
      661 ggttgctggg tgtggcggcg aaatgcgcta cggcgcgccc ggagtcgtcg aatatcacca
      721 gagtggtggc agggtgggtg gcgaagtcgt agacccagtc gggcagggcg tcgctgagcc
      781 cggcgtcgac ctccaccccg agccccgcca gggcggcgcg atgctcctcg gccgggacga
      841 cggtctcgta gtcctctgtc agggcctcgc ccgccgccag gaagagcggt atcggtgcat
      901 tcgccgcctc gagcaccgtt cgagcggcgc cgggggtctt ggagacggcc accggttgcg
      961 agaaagcatc caagatcaga actgcgaacc aggtaccggt caagtcgtac ttggagagga
     1021 actcggcagg gagttgatag tctgcggaca gtccagtgaa ctcacccttg gcgcacgcct
     1081 ctacgaagcg tccgagagcg gcagcggtag ggcgccactg tcgctgggat ctagcccagc
     1141 cgagtatgtg ctcgttgtcg gcgcagagca c
//
LOCUS       DAOQYE010000858         1170 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_161480_length_1170_cov_1.862780, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000858 DAOQYE010000000
VERSION     DAOQYE010000858.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1170
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_161480_length_1170_cov_1.862780"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="PKN52_11780"
     CDS             <1..418
                     /locus_tag="PKN52_11780"
                     /inference="COORDINATES: protein motif:HMM:NF013841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNR00678.1"
                     /translation="RTAGEVRHVFSKHGGNMSGSTAWQFDTKGMITVEQVGDAVEEAA
                     IELGADDLESDDGTSVIYTAAADLYAVVEGLQARGLPVDVVQITKLPQTQTALTAHEA
                     EKVMNFLESLEDLDDVQNVYTTADLDSIGESVDAAG"
     gene            437..1147
                     /locus_tag="PKN52_11785"
     CDS             437..1147
                     /locus_tag="PKN52_11785"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HNR00679.1"
                     /translation="MSFPHVRWDDLPAINANQMQQLIMLATGKFGLDNRVLVEHTARN
                     LVELVDAFGSDGPVLVVAGRGNNGSGGLAAARLLAARGRRVWVVPTHEAENYSGTPKE
                     QLEHLRHFERVKVRTSLPKMKFGCIIDAAIGTNLEGPPRGRTLDVITVLNHAQTDCMV
                     ISLDTPTGLNVDDGSTPGDVVHATMTLSIALPKVGVRPGGHVGRLFVGDLALPPGLME
                     ALRLPPVELPGFVTEVDA"
BASE COUNT          215 a          403 c          376 g          176 t
ORIGIN      
        1 ccgcaccgcc ggcgaagtca ggcacgtgtt cagcaaacac ggcggcaaca tgtcgggttc
       61 caccgcctgg cagttcgaca ccaagggcat gatcaccgtt gagcaggtgg gtgacgccgt
      121 cgaggaggcc gccatcgaac tcggcgccga tgacctcgag tccgacgacg gtacctccgt
      181 catctacacg gccgccgccg acctgtacgc ggtcgtcgag gggcttcagg cccgcggtct
      241 ccccgtcgac gtggttcaga tcacgaaact gccgcagacg cagacggctc tcaccgctca
      301 cgaggccgag aaggtcatga acttcctcga gagcctcgaa gacctcgacg acgtgcagaa
      361 cgtctacact accgccgacc tcgacagcat cggcgagagc gtcgacgccg ccggctaagc
      421 cgcagaaagg gtgtgcatga gcttcccgca cgtccgctgg gacgacctgc cagccatcaa
      481 cgcgaaccag atgcagcaac tgatcatgtt ggcgaccggc aagttcgggc tcgacaaccg
      541 ggtgctggtg gagcacacgg ctcgtaacct ggtcgagctc gtcgacgcct tcggctcgga
      601 cggtcctgtg ctcgtcgtgg ctgggcgcgg caacaacggc agcggaggcc tggctgccgc
      661 caggttgctc gccgcccgag gaaggcgcgt ctgggtcgtt cctacccatg aggccgagaa
      721 ctactccggc acgcccaagg agcagctaga gcatctgcgc catttcgagc gcgtcaaggt
      781 gcgcacgagc ctgccgaaga tgaagttcgg ctgcatcatc gacgccgcga tcggcacgaa
      841 cctcgagggc ccgcctcgcg gccggacgct ggacgtcatc acggtcctca accacgcgca
      901 gaccgactgc atggtcatct cgctcgatac gccgaccggc ctcaacgtcg acgacggcag
      961 cacgcccggc gacgtcgtgc acgccaccat gaccctcagc atcgctctcc ccaaggtcgg
     1021 ggtgaggcca ggtgggcacg tcggcaggct gttcgtcggt gacctcgcgt tgccccctgg
     1081 gctgatggag gcgctgaggc tgccgcccgt cgagctgccc gggttcgtca ccgaggtgga
     1141 cgcctaacag cgcaagcgac ttgaggaccc
//
LOCUS       DAOQYE010000859         1168 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_161876_length_1168_cov_2.362983, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000859 DAOQYE010000000
VERSION     DAOQYE010000859.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1168
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_161876_length_1168_cov_2.362983"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="PKN52_11790"
     CDS             <1..546
                     /locus_tag="PKN52_11790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00680.1"
                     /translation="YYVQQQWYLAVTGLPLSYGTADVGDPERLRTVLVIANPAIQKRC
                     IDAARTFWHEHVLTGIPPEPSGTEDDARILRQTYRGTIPDPPLVLDAPEAAGLVSTYL
                     AAKAASDAAKREAETAKQKLCALMGEHERALAGGYLLSWKAQERTTIDTKALRAAHPA
                     IAGEFTRTTETRVFGTPKETR"
     gene            543..>1168
                     /locus_tag="PKN52_11795"
     CDS             543..>1168
                     /locus_tag="PKN52_11795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HNR00681.1"
                     /translation="MTDSQAVATRDDAQQAVERRPQNVLSVFGSASFREQVATALPAH
                     IGVDSMMRIALTEVRKQPELEKCTVPSFMGALLTAAQLGLRPGYNGEGWLIPRWNKNL
                     GSLEAQFQPGYRGLAQLAYRSGEVVDITAEPVYAADHFRYTLGSSPHIEHVPDMEAEH
                     RDADIRAFYAVIRLKSGGHIMKVLRRADADEVRDRFGPHNRQGQTVGP"
BASE COUNT          200 a          434 c          385 g          149 t
ORIGIN      
        1 tactacgttc agcagcagtg gtacctcgcc gtcaccggtc tgccgctgtc ctacgggacc
       61 gccgacgtgg gcgacccgga gcggctgcgt acggtgctcg tcatcgccaa ccccgccatc
      121 caaaagcgct gcatcgatgc ggcgcggacg ttctggcacg agcacgtgct gaccggcatc
      181 ccgccggagc cgagcgggac cgaggacgac gcccggatac tgcgccagac gtaccgcggc
      241 accatccccg acccgccgct ggtgctcgac gcgcccgaag cggcagggct cgtctctacg
      301 tacctcgccg ccaaggccgc ctccgatgcc gccaagcgcg aggccgagac ggcgaagcag
      361 aagctctgcg ccctgatggg cgagcacgag cgggcgctgg ccggcggcta cctcctgagc
      421 tggaaggcgc aggagcgcac caccatagac accaaggcac tccgagccgc gcacccggct
      481 atcgcgggcg agttcacccg cacgaccgag acgcgcgttt tcggtactcc caaggagaca
      541 cgatgaccga ctcacaggcc gtcgctacgc gcgacgacgc acagcaggcc gtcgaacgac
      601 ggccgcagaa cgtcctctcc gttttcggca gcgccagctt ccgcgagcag gtcgctacgg
      661 cgctcccggc ccacatcggc gtggactcga tgatgcgcat cgcgctgacc gaggtccgca
      721 agcagccgga gctggagaag tgcaccgtgc cgagcttcat gggcgcgctg ctcaccgccg
      781 cccagctcgg cctccggccg ggctacaacg gcgagggctg gctcatcccg cgctggaaca
      841 agaacctagg ctcacttgag gcgcagttcc agcccggcta tcgcggcctc gcccagctcg
      901 cctaccggag cggcgaggtc gtggatatca ccgccgagcc ggtgtacgcg gccgaccact
      961 tccgctacac gctcggctcg tcgccgcaca tcgagcacgt gcccgacatg gaggccgagc
     1021 accgcgacgc cgacatccgg gcgttctacg cggtcatccg gctgaagtcc ggcgggcaca
     1081 tcatgaaggt gctgcgccgc gccgacgccg acgaggtgcg ggaccgcttc gggccgcaca
     1141 acaggcaggg ccagaccgtg ggcccgtg
//
LOCUS       DAOQYE010000860         1167 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_162035_length_1167_cov_3.688849, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000860 DAOQYE010000000
VERSION     DAOQYE010000860.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1167
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_162035_length_1167_cov_3.688849"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..347
                     /locus_tag="PKN52_11800"
     CDS             <1..347
                     /locus_tag="PKN52_11800"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNR00682.1"
                     /translation="TTFGGNPLACAAGLAVLDEFQRGELLVNVREMGERLAGGLRAVR
                     SPRVRDVRGRGLMVGMELRERVGPVIRGLRERGLLTVAAGATVIRFLPPLTVGPLEVD
                     RAIEIVAEELGA"
     gene            469..>1167
                     /locus_tag="PKN52_11805"
     CDS             469..>1167
                     /locus_tag="PKN52_11805"
                     /inference="COORDINATES: protein motif:HMM:TIGR01902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNR00683.1"
                     /translation="MSDEKAVELIRRSVATASLSGGERPLAEYLVGVVAGSASQAFVD
                     GAGNAVAVWGSGPCRVTFLGHLDTVAGEIPVRIEDGVLYGRGSVDAKGSLCAALAAAT
                     RVPSAVWDRLTFTFIGAVEEEAPSSKGARHAVAAYPKPDVLVVGEPSGWERYTLGYKG
                     RLGVRLTARCANAHSAREDASAAELVVDAYTRLREWVGRDNEGVEGLFERLQLTLLGL
                     ASGEDGLEAWCEAHA"
BASE COUNT          158 a          364 c          469 g          176 t
ORIGIN      
        1 gcaccacctt cgggggcaac cccctcgctt gcgccgccgg cttggcggtg ctggacgagt
       61 tccaacgcgg agagttgctg gtgaacgtca gggagatggg ggagaggctc gccggcggac
      121 tgcgcgccgt tcgtagtcca cgcgtgcggg acgtgcgcgg acgcggactg atggtcggca
      181 tggagctacg ggagcgagtg ggcccggtca tcaggggcct gcgtgagcgc gggctgctca
      241 ccgtggcggc cggagcgacc gtcatccgtt tcctgccgcc cctcacggtc ggtccgctgg
      301 aggtagaccg ggctatcgag atcgtggcgg aggagctcgg agcgtgagat tggacgcctg
      361 gctcgccacg gcggtgctca ccgcatgggc tgctcgcgag ccgtgagttc gggagttgcg
      421 ggctcgagga ccgggctccc cgagcctccg gtcgctgcgg acaccgggat gagcgacgag
      481 aaggccgtcg agctgattag gcggtccgtg gccacggcta gcctctcggg tggcgagcga
      541 ccactggccg agtacctcgt gggtgtcgtg gccggcagcg cctcgcaagc gttcgtcgac
      601 ggggccggca acgccgtggc cgtctggggc tcggggccgt gccgggtgac cttccttggc
      661 cacctcgaca cggtggcggg cgagatcccc gtgcgcatcg aggacggcgt gctgtacggc
      721 aggggctcgg tcgacgccaa gggtagcctc tgcgcagcgc tcgcggccgc aacgcgcgta
      781 ccgagcgcgg tgtgggaccg cctcaccttc acgttcatcg gcgcggtgga ggaggaggcc
      841 cccagcagca agggggcccg gcacgccgtg gcggcctatc cgaaaccgga tgtccttgtc
      901 gtgggcgagc cgagcggctg ggagcgctac acgctcggtt acaaggggag gctgggagtc
      961 aggctcacgg ctcgctgcgc caacgctcac tccgcccgcg aggacgcgag cgcggccgaa
     1021 ctggtggtgg acgcctacac gcggctgcgg gagtgggtcg gtcgcgacaa cgagggcgtg
     1081 gaagggctgt tcgaacgcct gcagctgacg ctgttgggcc ttgcctccgg cgaggacggt
     1141 ctggaggcgt ggtgcgaggc gcatgcg
//
LOCUS       DAOQYE010000861         1166 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_162397_length_1166_cov_1.941494, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000861 DAOQYE010000000
VERSION     DAOQYE010000861.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1166
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_162397_length_1166_cov_1.941494"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1166)
                     /locus_tag="PKN52_11810"
     CDS             complement(<1..>1166)
                     /locus_tag="PKN52_11810"
                     /inference="COORDINATES: protein motif:HMM:NF015213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HNR00684.1"
                     /translation="AREHLASGDPVEMVMALDGFRAAPLSPKSRDRLAAQLFRYLPLI
                     ATEYGRGVAGTDGEVRVTNEIEITEAKAFYQGALGNYRDLEPLVFELDAGAVPRLRAG
                     FEGLATALAAAGGQGAPGAPGGSDAGLLSAKAMQKAVNDLVSSLTTVVPADWRRGDAS
                     GDLEVIAEQLKVLAQAVAAGRYDLAESTRLDAYAVLESGPEARLRVFQPELSNRLEQL
                     FWHGAEPPGLARLIRDQASEAAVLATIGELNAGLREANAFLGTAASPAAVAANAAIIV
                     FREGLEAVLILAALLGSLKKPEVRRFRRPLWVGAIAALAATVVTWFALRGVLTEFARY
                     GEKLEAVVSVIALLVLLLITNWFFHQVYWKDHLANFHKRKAGLLGSSQYLGLALL"
BASE COUNT          183 a          401 c          420 g          162 t
ORIGIN      
        1 aggagggcca acccgaggta ctggctcgag ccgagcaggc cggccttgcg cttatggaag
       61 ttggcgaggt gatccttcca gtacacctgg tggaagaacc agttggtgat cagcaggagc
      121 acgagcagcg cgatgaccga cacgacggcc tcgagcttct ccccgtaacg ggcgaactcc
      181 gtgaggacgc cgcgcagagc gaaccacgtc accaccgtgg cggcgagcgc ggcgatcgcc
      241 ccgacccaga gcggcctgcg gaagcgccgc acttcgggct tcttgagcga gccgagcagc
      301 gccgcgagga tcagcaccgc ctcgagccct tcgcggaaga cgatgatcgc ggcgttggcc
      361 gccacggcgg cgggcgaggc ggccgtgccc aggaaggcgt tcgcctcacg gaggccggcg
      421 ttcagctcgc cgatggtggc gaggaccgcg gcctccgagg cctggtcgcg gatcaggcgc
      481 gcgagaccgg gcggttcggc gccgtgccag aagagctgct cgaggcggtt cgacagttcg
      541 ggctgaaaga cgcgcagccg ggcctccgga ccactctcga ggacggcgta ggcgtcgagc
      601 cgggtcgact ccgcgaggtc gtagcgcccc gcagcgacag cctgggcgag gaccttgagc
      661 tgctcggcta tgacctcgag gtcgccggac gcgtctccgc gtcgccagtc ggcgggcacg
      721 acggtggtaa ggctcgacac taggtcgttg accgccttct gcatggcctt cgcactgagg
      781 agccccgcgt ccgacccgcc aggcgcacct ggcgcgccct gaccaccggc cgccgccagc
      841 gcggtcgcca ggccctcgaa ccccgccctg agccgaggca cggctccggc gtccagctcg
      901 aagacgaggg gctcgaggtc gcggtagttg ccaagggccc cctggtagaa ggccttggcc
      961 tccgttatct cgatctcgtt cgtcacgcgc acctcgccgt cggtgcctgc cacgccacga
     1021 ccgtactctg tcgctatcag agggaggtaa cggaagagtt gcgcggcgag gcggtcgcgg
     1081 ctcttggggc tcagtggcgc ggccctgaag ccgtcgagcg ccatgaccat ctcgaccgga
     1141 tcgcccgacg cgaggtgctc gcgtgc
//
LOCUS       DAOQYE010000862         1164 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_162797_length_1164_cov_2.550947, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000862 DAOQYE010000000
VERSION     DAOQYE010000862.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1164
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_162797_length_1164_cov_2.550947"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1164)
                     /locus_tag="PKN52_11815"
     CDS             complement(<1..>1164)
                     /locus_tag="PKN52_11815"
                     /inference="COORDINATES: protein motif:HMM:NF037413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00685.1"
                     /translation="PVYWDDSHHPELSMTNGVPDGRWIFINGNNTPRIARIDLSTFET
                     VEIIEIPNIAGNHPSPFTTMNSEYVVAGTRFSVPVPQRDMPISEYKDNFKGMLTFVSV
                     DKASGEMAVAFQVMMPGFDYDLAHCGKGPSADWCFFTSYNSEQAHTLLEVNASQNDKD
                     FVAAVNWRRAEQCVADGKSSQLPSYYAHNLLDEATHTAVTTWHDSVTVLQPEECEGLV
                     YFLPTPKSPHGVDVDPTGRFIVTGGKLSATIAVHGFENMTKAIADQKIEGYSYGVPVL
                     DFDATVASQVERACLGPLHNEFDSKGNVYTSCFITSEIIKWDTKKFEVSDRIPVYYSI
                     GHLMIPGGDSMQPWDKYVVALNKITKDRYLPTGPELTQSAQLIDISGNTMQMLL"
BASE COUNT          181 a          312 c          435 g          236 t
ORIGIN      
        1 gagcagcatc tgcatggtgt tgccgctgat gtcgatgagc tgagcggact gggtgagctc
       61 agggccggtg ggcaggtagc ggtccttcgt gatcttgttg agcgcgacga cgtacttgtc
      121 ccagggctgc atgctgtcgc cgccagggat catcaagtgg ccgatggagt agtagacggg
      181 gatgcggtcg ctgacctcga acttcttcgt gtcccacttg atgatctcgg aggtgatgaa
      241 gcagctcgtg tagacgttgc ccttgctgtc gaactcgttg tgtagcggcc cgaggcaggc
      301 gcgctcgacc tggctggcga cggtcgcgtc gaagtcgagg acgggcacgc cgtaggagta
      361 cccctcgatc ttctggtccg cgatggcctt ggtcatgttc tcgaagccgt ggacggcgat
      421 ggtggcggag agcttgccgc ccgtgacgat gaacctaccg gtcgggtcga cgtcgacgcc
      481 gtgcggcgac ttgggcgtcg gcaggaagta gacgaggccc tcgcactcct ccggctgcag
      541 cacggtgacg gagtcgtgcc aggtcgtcac ggccgtgtgg gtggcttcgt cgaggaggtt
      601 gtgggcgtag taggagggga gttggctcga cttgccgtcg gcgacgcact gctcggcgcg
      661 gcgccagttg acggcggcga cgaagtcctt gtcgttctgc gaggcgttga cctcgagcag
      721 ggtgtgcgcc tgctcgctgt tgtacgaggt gaagaagcac cagtcggccg acgggccctt
      781 gccgcagtgc gcgaggtcgt agtcgaagcc gggcatcatg acctggaagg ccacggccat
      841 ctcgccgctc gccttgtcga ccgacacgaa cgtgagcatg cccttgaagt tgtccttgta
      901 ctcggagatg ggcatgtcgc gctgcggaac ggggacgcta aagcgcgtgc cggcgacgac
      961 gtactcgctg ttcatggtcg tgaagggcga ggggtgattg cccgcgatgt tggggatctc
     1021 gatgatctcg accgtctcga aggtggagag gtcgatcctg gcgatgcgcg gggtgttgtt
     1081 gccgttgatg aagatccagc gaccgtcagg caccccgttg gtcatggaga gctcggggtg
     1141 gtgcgagtcg tcccagtaga cggg
//
LOCUS       DAOQYE010000863         1162 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_163263_length_1162_cov_2.702800, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000863 DAOQYE010000000
VERSION     DAOQYE010000863.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1162
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_163263_length_1162_cov_2.702800"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            35..103
                     /locus_tag="PKN52_11820"
                     /pseudo
     CDS             35..103
                     /locus_tag="PKN52_11820"
                     /inference="COORDINATES: protein motif:HMM:NF015406.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding domain-containing protein"
     gene            complement(289..684)
                     /locus_tag="PKN52_11825"
                     /pseudo
     CDS             complement(289..684)
                     /locus_tag="PKN52_11825"
                     /inference="COORDINATES: protein motif:HMM:NF024872.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
BASE COUNT          160 a          454 c          403 g          145 t
ORIGIN      
        1 ggcgcaaagc ggctggatgc acaaccgggc gcgcatgatc accgcctcct tcctgaccaa
       61 ggacctgttc gtgccctggc aggagggggc ggcctggttc tgggacacgc tgtgcgacgc
      121 cgacctcgcc agcaactcgg cgagctggtc gtccgtcggt gtatcggcgc cgacgcgccg
      181 cataccctgc ctgaagagtt ccagcgccgc ctcctcggcg agttccagca cctcgatgac
      241 ccgctcgccg acgatgtgca gcggtctgcg cgcggtaacg aggagccgca gcccgggcga
      301 gcgttccacg aggcgcgcga gcacgcccgc ctcgtcggcc aaggcgtcgg cgttgtcgag
      361 cacgagcctc acgcgcgcgc ccgctagccg ctcggccagc tcctcggcat cgtcgacgcg
      421 gcgtagctcg agcgtctcgg cgatggccgc tggcagccgg gcggccgaat cgaccgcccg
      481 caagtccacc cagtgagtgc cgcccggcgc gtccggtgcc cggcccgtcg cgaggtcgag
      541 cgcgagttgg gctgccagcc tggtcttgcc ggagccaccg ttgccgacca gcgtcaccag
      601 cggctgctcg cggaccaagg gcgtggtggc ggcgagccag cgcgcgcgtc cgacgagagg
      661 cgcctcccaa cgcggcagct tcacccgagc cggctcatga gacccgccag cctcgggagc
      721 ttgcagccac gcgccgagcc gctcgcgcag cccgtcgagg tcggcgttcc accccgtatc
      781 gttccgacgc agcacgcccg cgccgaggag gtcgagcacg agcggcgcta tacgacccgg
      841 caggccgccg cccgcgcgag ccagcgcggc ggccaccggc tcggcgatcc gcccacctag
      901 cgcggcgccg agccaggccg cgatgtccgc ctccggcaaa ggcccgagct cgatgcgtcg
      961 gtcggcgccg aacgcggcgg actcgtcgtc ctgcacgacc tcgacgagcc gggttccgcc
     1021 ccggcgcaag ctctccgcca ccagcccgac gctcgccggg tcgaaccagc gcgcgccgct
     1081 cacaacgagc aggagcccat gggcttcggc atcgttgcgg aggcgttccc ggatatcgtc
     1141 ctccacggcc tcgctcggcc gg
//
LOCUS       DAOQYE010000864         1162 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_163343_length_1162_cov_2.079494, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000864 DAOQYE010000000
VERSION     DAOQYE010000864.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1162
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_163343_length_1162_cov_2.079494"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..545
                     /locus_tag="PKN52_11830"
     CDS             <1..545
                     /locus_tag="PKN52_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNR00686.1"
                     /translation="RLAVELGVGVEVLPGPTAFVPALVLSGLPTARFTFEGFLPRKGG
                     ERGRRLDAIAASGATSVLYEAPQRLSATLVDLARACGPERQASVSRELTKLHEETRRG
                     SLAELHEHYAATEARGEVVVVVGPALEPEEDGSSAATQAVALAGAGGRGRLLREALLA
                     LGVPRNEAYRLAIAHPDDDS"
     gene            601..>1162
                     /locus_tag="PKN52_11835"
     CDS             601..>1162
                     /locus_tag="PKN52_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00687.1"
                     /translation="MNDITAQPLVSAVEAGGRYLGLTAAARLMLFARAPGPAVLLTTS
                     DRAALFDDTHVFGALRTVDPTLAEWHERREKVVLTLGHAVGPFPEHPEAFALELTKGR
                     SYARDELLERLVNYGYVRDAAPGFTVRGDTVTLYREPLPESAVGEDTDDAEPAFPEAP
                     PGSVRLEFFGDELDALFVAGEEAQRMV"
BASE COUNT          161 a          402 c          430 g          169 t
ORIGIN      
        1 tgcggttggc cgtcgagttg ggggtcggcg tcgaggtcct gcccggcccg accgcgttcg
       61 tgcccgcgct cgtcctctcc ggcctcccga cggcgcgctt cacgttcgag ggcttcctgc
      121 cccgcaaggg aggggagcgc ggccgcaggc tcgacgcgat cgcggcctcc ggggcgacct
      181 ccgtgctgta cgaggccccg caacgcctgt ccgccaccct cgtcgatctg gcgcgcgcgt
      241 gcggtcccga gcgccaggcg agcgtgagtc gtgagttgac caagctgcac gaggagacgc
      301 gcagggggag tctggcggag ctgcatgagc attacgccgc caccgaggct cgcggggagg
      361 tcgtggtggt ggtgggcccc gccctggagc ccgaggagga cggctcgagc gcggctacgc
      421 aagcggtggc gctagccggg gcggggggtc gaggtaggct gttgcgggaa gcgctgctcg
      481 ccttgggggt cccgaggaac gaggcgtacc gcctggccat cgcccacccg gacgacgact
      541 cctgatcgga gacgaatgct ccggggggtt ccgcatgcct cgctaccgga aggtcatgta
      601 gtgaacgaca tcaccgcaca gcccctcgtc tcggccgtag aggccggcgg acgctatcta
      661 gggctcacgg ccgcggcgag gctcatgctc ttcgcgcgcg cgcccggccc ggccgtgctc
      721 ctgaccacga gcgaccgcgc agcgctgttc gacgacacgc acgtgttcgg cgccctgcgc
      781 accgtcgatc cgaccctggc ggagtggcac gagcgccggg agaaggtcgt gttgaccctc
      841 ggccacgctg tcgggccgtt ccccgagcat cccgaggcct tcgctctcga gctcaccaaa
      901 ggccgcagct atgcgcgcga cgagctactg gaacgcctcg tgaactacgg ttacgtccgg
      961 gacgcagccc ccggcttcac ggtgcgaggc gacacggtca cgctctaccg ggaaccgtta
     1021 ccggagagcg cggtgggcga agacaccgat gacgcggagc cggcgttccc cgaggcgcct
     1081 cccggcagcg tgcggctaga gttcttcggc gacgagctcg acgcgctctt cgtggccggc
     1141 gaggaggcgc agcgcatggt cg
//
LOCUS       DAOQYE010000865         1162 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_163367_length_1162_cov_1.956640, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000865 DAOQYE010000000
VERSION     DAOQYE010000865.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1162
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_163367_length_1162_cov_1.956640"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..895
                     /locus_tag="PKN52_11840"
     CDS             <1..895
                     /locus_tag="PKN52_11840"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNR00688.1"
                     /translation="GATGLEALALTTQLLQRARPAHPTAGLWEEADFQWWWRRPQPAD
                     QVEKVFWLDDDGPVAGALVTSWGPDSWQYDPVVVPGTPSPTRSAVWDRALALAATVTN
                     RTLENGQGTASGTGSAPAFEVGVMDGDREFEDLAGRAGLSPVRHDSTAWLAAERRPAV
                     IPLPKGFSLVDRTQRLDAPHHMRHRNGAEVAERLAQCSLYDPTLDIAIETDAGQMAAY
                     SLYWFDPVTKVGLVEPVRVEDAFQRRGLARAMLSAGLERVAAKGAQRMKVSYSSAPAA
                     ALYLGLGFEAESTTTWYSGAK"
BASE COUNT          182 a          393 c          428 g          159 t
ORIGIN      
        1 cggagcgacg ggtctcgaag cgttggcgct caccacgcag ctgctgcagc gcgcccggcc
       61 ggcacacccg acggccggcc tctgggagga agcggacttc cagtggtggt ggcgccgacc
      121 gcaaccggcc gaccaggtgg agaaggtctt ctggctggac gacgacgggc cggtggccgg
      181 tgccctcgtg acgtcgtggg gtccggacag ctggcagtac gaccccgttg tggtccccgg
      241 aacgccgagc ccgacgcgca gcgccgtctg ggaccgggcg ctcgcgctcg ccgcgacggt
      301 cacgaacagg acgctcgaga acgggcaggg taccgcgtcc ggtaccgggt ccgctcccgc
      361 gttcgaggtc ggcgtcatgg acggcgaccg ggagttcgag gacctcgccg ggcgcgccgg
      421 gctctccccc gtcaggcacg acagcacagc ctggctggcc gccgagaggc ggccggccgt
      481 tatcccgttg cccaaaggct tctcgctcgt cgaccgcacc caacggctgg atgccccgca
      541 ccacatgcgg caccgtaacg gcgcggaggt ggccgagcgc ctcgcccagt gctcgctcta
      601 cgacccgacg ctggacatcg ccatcgagac cgacgccggc cagatggcgg cctactccct
      661 ctactggttc gacccggtca cgaaggtcgg cctcgtcgag ccggtcaggg tcgaggacgc
      721 gttccagagg cgcgggctgg cgcgcgccat gctcagcgcg gggctcgagc gcgtggcggc
      781 gaagggcgcg cagcggatga aggtctcgta cagcagtgcg ccggccgcgg cgctctacct
      841 cgggctcggg ttcgaggcgg agtcgacgac cacgtggtac tcgggcgcga agtaggcacc
      901 gaccatggtc agcgaaatgc cgtcccaacc ggcataaacg acggagttgg gtggaagggc
      961 gtggtaccgt gagagggtgc cccacccggt cacaggcgtc gttgcgaacg aagataagac
     1021 gctaacggcc ttggcgtcga agtacatctg gtggcaggac gccgagtcgg gctggttcaa
     1081 cgaccgttct tggcactact ggcattaccg gctcgggctt gccgaactgg ccgccggcgg
     1141 cccggcgcgc gacctggaga ag
//
LOCUS       DAOQYE010000866         1161 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_163508_length_1161_cov_2.679024, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000866 DAOQYE010000000
VERSION     DAOQYE010000866.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1161
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_163508_length_1161_cov_2.679024"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            63..890
                     /locus_tag="PKN52_11845"
     CDS             63..890
                     /locus_tag="PKN52_11845"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNR00689.1"
                     /translation="MSGLLEVKGGAYTLPTGRVLFSGIDLGLRAGEILCVLGPNGVGK
                     TTLIHCVTGLKRWAAGATYLAGRSQASYSPREFWSKVGFVPQARANVFAYSVLEMVLL
                     GLSTRVGEFGVPSAAQRAQALAVLERLEIAHLARRSCNELSGGELQLVFIGRALVNEP
                     ELLILDEPESHLDFKKQLIILDLVRRLAVEDGIACIMNTHFPNNAMRVATQILLLGGG
                     ARYLVGDAASVLTRANVAEYFEVDARIVTVATDAGTWPALYPVSVIGDRGTTIGKDS"
     gene            904..>1161
                     /locus_tag="PKN52_11850"
     CDS             904..>1161
                     /locus_tag="PKN52_11850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00690.1"
                     /translation="MKFPRSGRLALAIAVAVASSLAAAQTRVVTDRMGRQVEVPVEIN
                     RVVTNWLPFPSAFFISTGSLDRLVAVGEDSIATANRSMLGRI"
BASE COUNT          179 a          425 c          366 g          191 t
ORIGIN      
        1 gccgccatga tcggcgcgcc cttcttcgcc ttccttctca tgcgcaaccg caaggactgg
       61 acgtgagcgg cctcctggag gtcaaaggtg gcgcctacac gctccccacg ggccgcgtcc
      121 tgttctcggg catcgacctc gggttgcgcg ccggcgagat cctgtgtgtg ctcggcccga
      181 acggggtcgg caagaccacc ctcatccatt gcgtcaccgg cctcaagcgc tgggcggcag
      241 gcgccactta cctcgccggg cgcagccaag cctcgtactc cccgcgggag ttctggagca
      301 aggtcgggtt cgtcccgcag gcccgcgcga acgtcttcgc gtactccgtg ctggagatgg
      361 tactgctcgg cctctcgacg cgcgttggcg agttcggcgt gccgagcgcg gcacagcggg
      421 cgcaggccct cgcggtcttg gagcggctcg agatcgcgca cctggcccgg cggagctgca
      481 acgagctgag cggcggcgag ctgcagctcg tgttcatagg ccgcgcactc gtgaacgagc
      541 ccgagctcct catcctcgac gagccggagt cgcatctcga cttcaagaaa cagctcatca
      601 tcctcgacct agtcaggcgg ctcgcggtcg aggacggcat cgcgtgcatc atgaacacgc
      661 acttccccaa caacgccatg cgcgtcgcga cccagatcct gctgctcggc ggcggtgcgc
      721 gctacctcgt cggcgacgcc gccagcgtgc tgacgcgcgc caacgtcgcg gagtacttcg
      781 aggtcgacgc gcgcatcgtc accgtcgcca ccgacgccgg cacgtggccg gccctctacc
      841 ccgtctcggt catcggcgac cggggcacca ccatcggcaa agacagctaa ggaggctgta
      901 agcatgaagt tccctcgtag cggcaggcta gccctcgcca tcgcagtggc cgtcgcgtca
      961 tccctcgcgg cggcgcagac ccgcgtcgtc accgaccgca tgggacgcca ggtagaggta
     1021 ccggtcgaga tcaaccgtgt cgtcacaaac tggctcccgt tcccgtccgc cttcttcatc
     1081 tccaccggct cgctcgaccg actcgtggct gtcggggagg actcgatcgc gaccgccaac
     1141 cgctccatgc tcggcaggat c
//
LOCUS       DAOQYE010000867         1159 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_163911_length_1159_cov_3.926630, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000867 DAOQYE010000000
VERSION     DAOQYE010000867.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_163911_length_1159_cov_3.926630"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..846
                     /gene="metK"
                     /locus_tag="PKN52_11855"
     CDS             <1..846
                     /gene="metK"
                     /locus_tag="PKN52_11855"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HNR00691.1"
                     /translation="DRADDGSDDAKERIGAGDQGLMFGYAIDETPELMPLPILLAHRV
                     AQRLAEVRKSGLLPYLRPDGKAQVTIEYQDGVATRLRNAVLSAQHHPDVPMDELKADL
                     LEHVLRVALPAEFFDDGTRVYINPTGRFVEGGPTADAGLTGRKIIVDTYGGAAPHGGG
                     AFSGKDPTKVDRSASYYARYIAKNVVAAGLAKRCQVQLAYAIGVARPVGMYVDTFGTG
                     ALPDDRLAEVVTAEFDARPAAIIEQLDLRRPIYAATSAYGHFGRAGFPWEETDRAARL
                     RAAVA"
     gene            914..>1159
                     /locus_tag="PKN52_11860"
     CDS             914..>1159
                     /locus_tag="PKN52_11860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00692.1"
                     /translation="MARIVLGNREGLLAIRHGRSVLELLTDEWPDLQLRLRTVSKTGG
                     QESGPLLQALAGGEVGIAVAQLECLPPTLPDGLKLAAV"
BASE COUNT          184 a          423 c          382 g          170 t
ORIGIN      
        1 gatagggccg acgacggcag cgacgacgcc aaggagagga tcggggccgg cgaccaaggc
       61 ctcatgttcg gttacgccat cgacgagacg cccgagctca tgccgctgcc gatcctccta
      121 gcccaccggg tggcgcagcg cttggccgag gtacgcaaga gcggcctgct tccctacttg
      181 cgccccgacg gcaaggccca ggtgaccatc gagtaccagg acggcgtcgc gactcgcctg
      241 cgcaacgccg tcctctctgc ccaacatcat ccggacgtcc cgatggacga gctcaaggcc
      301 gacctcctcg agcacgtgct gcgcgtggcg ctgcccgccg agttcttcga cgatggcacg
      361 cgcgtgtaca tcaacccgac agggcggttc gtcgagggcg ggcccaccgc ggacgcgggt
      421 ctgaccgggc gcaagatcat cgtcgacacc tacgggggag ccgccccgca cggtggcggg
      481 gcgttcagcg gcaaggaccc caccaaggtc gaccggtcgg ccagctacta tgcccgctac
      541 atcgccaaga acgtcgtggc cgccgggctc gccaaacggt gtcaggtgca gctcgcctac
      601 gccatcggcg tggcgcggcc ggtaggcatg tacgtggaca cgttcggcac gggggcgtta
      661 ccagacgacc gcctcgccga ggtcgtgacc gccgagttcg acgcccggcc ggccgcgatc
      721 atcgagcaac tcgacctgcg ccgcccgatc tacgccgcta ccagcgcgta tggccacttc
      781 ggccgtgccg ggttcccctg ggaggagacg gaccgcgccg ctcgcctccg cgccgcggtg
      841 gcctagggat aagccgcgcc agcacgcgct ttctcgtctc gcgcacttcg gggttcgtgg
      901 tacaaatagc gccatggcca ggatagttct gggcaatcgt gagggcctac tcgccatccg
      961 tcacggtcgc agcgtgctcg agctcctcac ggacgagtgg cccgacctcc aactacgcct
     1021 tcgcaccgtc tcgaagaccg gcggtcagga gagcggtccc ctcctccagg ctctcgccgg
     1081 tggcgaggtc ggcatagccg tcgcccagct ggagtgcctg ccccctaccc tgccggacgg
     1141 gctcaagctg gccgccgtc
//
LOCUS       DAOQYE010000868         1159 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_163966_length_1159_cov_2.769928, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000868 DAOQYE010000000
VERSION     DAOQYE010000868.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_163966_length_1159_cov_2.769928"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            8..793
                     /locus_tag="PKN52_11865"
     CDS             8..793
                     /locus_tag="PKN52_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-6-naphthoate synthase"
                     /protein_id="HNR00693.1"
                     /translation="MFHALTHGLVPGAQEFEFDVLLDDVEAMNRLVAAGRLDVAKISY
                     HAYGYVAQDYVLLRSGGALGRGVGPLVVAAAPELDLTGKRVAVPGGRTTAKLLLELWR
                     PAGLELTELRYDAIMPAVARGEVDAGLIIHESRFTYRSHGLHALVDLGAWWESEVGGL
                     VPLGAIVARRSLGPGTHARLARLIRASLDHAWRDPAASADYVAANAQEMAPDVRAKHI
                     GLYVNEYSHDVGEEGERAVRRLLGLGHAKGLFPALPEPLFAAA"
     gene            842..1069
                     /locus_tag="PKN52_11870"
     CDS             842..1069
                     /locus_tag="PKN52_11870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00694.1"
                     /translation="MVSFSVGEEVLYKDERYVISTASPRPPYQYRLLATTPEGAKVQW
                     ALASELTKMERYTRPRDDTRSIKSSAGQRRH"
BASE COUNT          164 a          419 c          412 g          164 t
ORIGIN      
        1 cacgttcatc ttccacgcgc tcacgcacgg actggtgccc ggcgcccagg agttcgagtt
       61 cgacgtcctg ctggacgacg tcgaggccat gaaccggctg gtggcggctg gccggctgga
      121 cgtggcgaag atcagctatc acgcctacgg ctacgtggcg caggactacg tgctgctgcg
      181 cagcgggggt gcgctcggac gcggcgtggg gccgttggtc gtggcggccg ccccggagct
      241 cgacctgacc ggcaagcgcg tggccgtacc gggcggccgc acgacggcga agctcctgct
      301 cgagctctgg cgccccgccg gtctggagct cacggagctg cgctacgacg ccatcatgcc
      361 cgccgtggcg cgcggcgagg tcgacgccgg cctgatcatc cacgagagcc gcttcaccta
      421 ccggtcgcac ggcctgcacg cgctcgtcga cctcggcgcc tggtgggagt ccgaggtggg
      481 cgggctggtg cccctcggcg ccatcgtcgc ccggcgctcc ctcgggcccg gcacgcacgc
      541 acgcctcgcc aggctcatcc gggcgagcct tgaccacgca tggcgggacc cggcggcgtc
      601 cgccgactac gtggcggcca acgcgcagga gatggcgcct gacgtgcgcg ccaagcacat
      661 cgggctgtac gtgaacgagt actcgcacga cgtgggcgag gagggggagc gcgccgtacg
      721 gcgcctgctc gggctcgggc acgccaaggg cctcttcccc gccttgccgg agccgctgtt
      781 cgcggcggcc tagggtccaa agcccctgcc gctgtcgccc cgccagggtt aggcttggcg
      841 catggtgagc ttctcggtcg gcgaggaagt cctgtacaag gatgagcgtt acgtcatcag
      901 cacggcctcg ccgcgccctc cgtaccagta ccgcctgttg gccacgacgc ccgagggcgc
      961 gaaggtccag tgggcgctcg cttccgagct gacgaagatg gagcgttaca cgcgcccgcg
     1021 cgacgacacc cgctccatca agagcagcgc cggccagcgg agacactgat cctgtcagcc
     1081 tgacgcggcg agcgctgatc cgagcgccat gccgtcgctc acggccgtgt cgtaaccgaa
     1141 acgccccacg caggcgccg
//
LOCUS       DAOQYE010000869         1158 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_164317_length_1158_cov_1.777879, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000869 DAOQYE010000000
VERSION     DAOQYE010000869.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1158
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_164317_length_1158_cov_1.777879"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..933)
                     /locus_tag="PKN52_11875"
     CDS             complement(<1..933)
                     /locus_tag="PKN52_11875"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNR00695.1"
                     /translation="MTLLERLVNIDSPSSAPGGTTAVQTVIAEQLERLGFSIRWHAVG
                     PEDGRFALEANYDTSSSDRVTLMGHVDTVFPIGTAARRPFSLVGKRAHGPGVSDMKAG
                     LALMTAAAAALVRGGALDAVPSVRVVLTSDEELGTPYSKEVIRELVDGSRCVLNCEPG
                     RHNGAVVTSRRGSAHLLLETTGVEAHSGTDFLKGRSAVVSLARGIVELEALNQLPGDQ
                     TINVGLVSGGTATNVVPGHAAAKVHVTFSEEDAGEAIIDLARAAVARAAIPGTRAELT
                     GGISFLPMPATLQGERLFDTYRDVTALLGLQVTSE"
BASE COUNT          228 a          379 c          355 g          196 t
ORIGIN      
        1 ctctgaagtc acctgaagac ctagcagtgc cgtgacgtcc ctataagtgt cgaatagccg
       61 ctctccttgt agggtcgcgg gcatgggcaa gaaactgatg ccaccggtca gttcggccct
      121 ggtaccgggg atcgcagcgc gagcgacggc ggctcgcgcc aaatcgatga tcgcctctcc
      181 ggcgtcctct tccgagaacg ttacgtgcac cttggcggcg gcatggccag gcacaacgtt
      241 cgttgcggta ccgccagaca cgagaccaac gttgatggtc tggtcgcccg gtagctggtt
      301 cagcgcttcg agctccacga tgcctctcgc caggctcacc acggcgctcc ggcctttcag
      361 gaagtccgtg cctgagtgcg cctcgacacc cgtggtctcg agaagaagat gcgcggagcc
      421 acgccgtgag gtgacgacgg ctccgttgtg acgcccaggc tcacagttca gtacgcacct
      481 gctgccgtct accagctcgc ggatcacctc cttggagtag ggcgttccga gctcttcgtc
      541 tgatgtcagg accacacgaa cagaggggac cgcgtcgagc gcaccgcctc gaacgagagc
      601 ggcggcagcg gccgtcatga gggccagccc ggccttcata tccgagacac ctggtccatg
      661 tgcgcgttta cccaccaggc tgaaaggccg ccgcgcggca gtgccgatcg ggaaaaccgt
      721 gtcaacgtga cccatgaggg tcacgcggtc tgatgacgaa gtgtcgtagt tggcttccag
      781 ggcgaaccga ccatcttccg gtcccaccgc gtgccagcga atcgagaaac caagacgctc
      841 cagttgctcg gcaatgaccg tctgcacggc cgtcgtaccc ccaggagcgc tcgatgggct
      901 gtcgatgttg acgagacgct caagaagggt caaagcctcc ggggtggcgg aagtcgcccg
      961 ctccaagacc gtccttacga gacgcgagtc caacgtgact cctcctcccg ctcaacccgc
     1021 gtcgagcacc agagacagcg tgactgccgc agtgatgctg acgggagcca ggaccggaac
     1081 cctgacggac gcctgcactc gccgttgcca ggtcagattg aacccgaaac accgaaggta
     1141 tatcagaggg caaccatc
//
LOCUS       DAOQYE010000870         1156 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_164610_length_1156_cov_3.434151, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000870 DAOQYE010000000
VERSION     DAOQYE010000870.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1156
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_164610_length_1156_cov_3.434151"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="PKN52_11880"
     CDS             complement(<1..423)
                     /locus_tag="PKN52_11880"
                     /inference="COORDINATES: protein motif:HMM:NF040248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNR00696.1"
                     /translation="MLVQTLSFIVTLWAVLTLVFIAMRALPGDAATIMGGIDATESET
                     SVLRSQLGLDRSLPVQYADYLADVVQGDLGNSLRERRPVVRVLLDRLPVTVTLASMAF
                     VIALGLGLGLGLLAGLRPGGTLDRMTLLFTTVALALPEF"
     gene            complement(611..>1156)
                     /locus_tag="PKN52_11885"
     CDS             complement(611..>1156)
                     /locus_tag="PKN52_11885"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNR00697.1"
                     /translation="ESVQPFPYDPEKAKALLKDAGVPADWTIRLVLPQPYPQHIQAGQ
                     IVQDFLSQIGVKAEISIVEWGVWLSDVYGGAHEFDITVVGHTGKLDPSGRLTGLGTDR
                     NYSGVIDDDVANWIAQANSNPDPAMRKSLYSQALIRLHDDAPFIYFGTAERVYARKAS
                     VQGFWITPLLDSFDFRATHFE"
BASE COUNT          191 a          351 c          433 g          181 t
ORIGIN      
        1 gaactcgggc agcgcaagcg cgaccgtggt gaagagcagc gtcatccggt cgagggtgcc
       61 gcccggtcgg aggccggcga ggagccccag ccccaggccc aggcccagcg ctatcacgaa
      121 ggccatgctg gcgagcgtga cggtgacggg taggcggtcg aggagcacgc gcaccactgg
      181 tcgccgctcg cgcagcgagt tcccgaggtc gccttgcacg acatcggcga ggtagtcggc
      241 gtactgaacg ggaagtgagc gatcgagacc gagctgcgag cgcagcacgc tcgtctccga
      301 ttcggtggcg tcgatgccgc ccatgatcgt ggcggcgtcg cccggcaggg cgcgcatcgc
      361 tatgaacacg agcgtcagta ccgcccatag cgtgacgatg aacgagaggg tctgtacgag
      421 caagtacctg gccacgttag tgagtcgcga gccgcctcgc cgctgacgtg gcgacgacgc
      481 gggcggggtg ccaggcggct ccacccaccg ggtggggtct agcggtggac cccacccgcg
      541 tcgatcgtcc tgtcagctca accgggtcgc ctgtgggcgg gttgatgcta cccgcccaca
      601 ggcctggcgg tcactcgaag tgagtcgccc tgaagtcgaa ggagtcgagg aggggcgtga
      661 tccagaagcc ctggacgctc gccttcctcg cgtagacgcg ctcggccgtg ccgaagtaga
      721 tgaagggcgc gtcgtcgtgc aggcggatga gggcctgcga gtacaaggac ttgcgcatgg
      781 ccggatcggg gttggagttg gcctgggcga tccagttggc gacgtcgtcg tcgatgacgc
      841 cgctgtagtt acggtcggtg ccgaggccgg tgaggcggcc ggacgggtcg agtttgcccg
      901 tatggcccac cacggtgatg tcgaattcgt gcgcgccgcc gtagacgtcg gagagccaga
      961 caccccactc gacgatggag atctcggcct tgacgccgat ctggctgagg aagtcctgca
     1021 cgatctgccc ggcctggatg tgctgagggt acggctgcgg gaggacgagc ctgatcgtcc
     1081 agtcggcggg aacgccggcg tccttgagga gcgccttggc cttctcggga tcgtagggga
     1141 acggctgcac agactc
//
LOCUS       DAOQYE010000871         1153 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_165351_length_1153_cov_2.731330, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000871 DAOQYE010000000
VERSION     DAOQYE010000871.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1153
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_165351_length_1153_cov_2.731330"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="PKN52_11890"
     CDS             <1..251
                     /locus_tag="PKN52_11890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00698.1"
                     /translation="QADSEARLADAARLLDRHALTVGDLQAVTAHEANICHRGEAPRF
                     VGTCGGVVTRPAGREMWAVRGLPSEGSYQRFAFEDVPA"
     gene            248..937
                     /locus_tag="PKN52_11895"
     CDS             248..937
                     /locus_tag="PKN52_11895"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNR00699.1"
                     /translation="MSGARPRPRLRIAQALPEHAVALAELQRVCFPNLAEHELMTEAH
                     FLEHQERFPEGEFVALAADAPDGTPLPEERVVGLGSGFLIDFDLQRPEHVFHDIIAGG
                     TYANHDPDGAWYYGADISVHPDYRGLGLGRALYDARKELVRRLGRKGIVAGGQLPGYP
                     RYRERLTVAEYVEKVVAGEIADATLTFQLANGFEVYGLIHGYIEDAYTDDWASFIVWF
                     NPDLHFPAFEE"
BASE COUNT          171 a          423 c          399 g          160 t
ORIGIN      
        1 cgcaggccga ctcggaagcg cgcctggccg acgcggcgcg gctcctcgac cgtcacgccc
       61 tcacggtggg cgacctgcag gccgtcacgg cgcacgaggc caacatctgc caccgcggcg
      121 aggcgccccg cttcgtgggc acctgcggcg gcgtggtcac gcgccccgcc ggccgcgaga
      181 tgtgggcggt gcgggggctc ccaagcgagg ggtcgtacca gcggttcgcg ttcgaggacg
      241 tgcctgcgtg agcggcgcgc gcccgcgacc gcgactccgc atcgcgcagg cgctgcccga
      301 gcacgccgtg gcgctggcgg agctgcagcg ggtgtgcttc cccaacctcg cggagcacga
      361 gctcatgacc gaggcgcact tcctggagca ccaggagcgc ttccctgagg gtgagttcgt
      421 ggcgctggcg gccgacgccc cggacggcac gcccctgccg gaggaacggg tcgtcgggct
      481 cgggtccggc ttcctcatcg acttcgacct gcagcgccct gagcacgtgt tccacgacat
      541 catcgccggc gggacctacg ccaaccacga cccagacggc gcttggtact acggcgctga
      601 catcagcgtt caccccgact accgcgggct cggccttggt agggccctgt acgacgcccg
      661 caaggagctc gtcaggcggc tcggtcgcaa gggcatcgtg gcgggcggcc agctgccggg
      721 ctacccccgc taccgcgagc ggctcaccgt cgccgagtac gtcgagaagg tcgtggccgg
      781 cgagatcgcc gacgctaccc tcacgttcca gctcgccaac ggtttcgagg tctacggcct
      841 gatccacggc tacatcgagg acgcctacac ggacgactgg gctagcttca tcgtctggtt
      901 caaccccgac ctgcacttcc ccgcgttcga ggagtagctg accaagcgac cgcggacctg
      961 agcgcgggtc ggtgttctcc tagatcgcta aggagtagac ggacatgacg aacgaacggc
     1021 cggccgccat gccgccggcg ggccacatcg ctgccccgcc gcacctcgat gacctcgcgg
     1081 aggcggccac ggtcgtggcc gcttacgagc tggcgcacga gggggtcgcc cataccaccg
     1141 ccgagacgct ggc
//
LOCUS       DAOQYE010000872         1151 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_165899_length_1151_cov_2.533759, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000872 DAOQYE010000000
VERSION     DAOQYE010000872.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1151
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_165899_length_1151_cov_2.533759"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1041
                     /locus_tag="PKN52_11900"
     CDS             <1..1041
                     /locus_tag="PKN52_11900"
                     /EC_number="4.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF005758.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-lyase"
                     /protein_id="HNR00700.1"
                     /translation="PVQATLSPASTYFLPGTPNAKHQYGRWSNPTWEVLEVALAILED
                     APVAIFPSGMAAIASVFMTHLGTGDRVLLPSDGYYTTRVLVEKYLAPHGVEVELRATA
                     DYATQDLSGFKLVFIETPSNPGLDICDVAEVAGRAKAAGALVVADNTTMTPLGQRPLN
                     LGADLVVASDTKALNGHSDVLFGHVAARDAGLLEPVRDWRKFVGAIPGPFEAWLVHRG
                     LESFEVRYERMTTTAAVLAERFSAHPAIRSVAYPGLATHPGHEIAKRQMTRFGTIIGL
                     TFADAAAADRFIENTSLIVPATSFGGVHSSAERRARWGDKVAEGYVRLSVGCEPTEAL
                     WEEINAVLDALA"
BASE COUNT          181 a          407 c          383 g          180 t
ORIGIN      
        1 cccgttcagg cgacgctgtc gcccgcgagc acctacttcc tccccggcac tcccaacgcc
       61 aagcaccagt acggtcggtg gagcaacccc acgtgggagg tcctggaggt cgctctcgcc
      121 atcctcgaag atgcgccggt cgcgatcttc ccttccggga tggccgccat cgcttcggtg
      181 ttcatgacgc acctcggcac cggggaccgc gtgctgctgc cgagcgacgg ctactacacg
      241 acccgcgtcc tcgtcgagaa gtacctcgcc ccgcacggcg tcgaggtgga gctgcgcgcg
      301 acggcggact acgccacgca ggacctctcc ggtttcaagc tcgtcttcat cgagacgccc
      361 tccaacccag ggctggacat ctgcgacgtg gcggaggtag ccggccgggc caaggcggcg
      421 ggagccctcg tcgtcgccga caacaccacc atgaccccgc tgggccagag gccgctgaac
      481 ctcggcgccg acctcgtagt cgcgtccgac accaaggctc tcaacggtca ctcggacgtc
      541 ctgttcgggc acgtcgccgc gcgcgacgcc ggcctgctcg agccggtccg ggactggcgg
      601 aagttcgtcg gcgccatccc cggtccgttc gaagcttggc tcgtgcaccg cgggctggag
      661 agcttcgaag tccgctacga gcggatgacc acgacggccg ccgtcctggc cgagcggttc
      721 tcggcgcacc cggcgatcag gtccgtcgct tatccgggcc tggcgacgca ccctggccac
      781 gagatcgcca agcggcagat gacgaggttc ggaacgatca taggtctgac cttcgccgat
      841 gcggccgcgg ccgaccggtt catcgagaac acgagcctca tcgtgcctgc cacgagcttc
      901 ggcggcgtgc atagctcggc cgagcggcgc gcccggtggg gcgacaaggt cgcggaaggc
      961 tacgtgcgac tgtccgttgg ctgcgagccg accgaggcgc tgtgggaaga gatcaacgcc
     1021 gtgctggacg cgctcgcctg acggcctagc gcagacgcta gcaacacttc atcggaggct
     1081 ttgcgcgagt ccgatgagga tgccttcggg tccgcggagg tagcagagcc tgtacgcgtc
     1141 ctggtactgc a
//
LOCUS       DAOQYE010000873         1151 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_165917_length_1151_cov_2.411496, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000873 DAOQYE010000000
VERSION     DAOQYE010000873.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1151
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_165917_length_1151_cov_2.411496"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            528..>1151
                     /locus_tag="PKN52_11905"
     CDS             528..>1151
                     /locus_tag="PKN52_11905"
                     /inference="COORDINATES: protein motif:HMM:NF019139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HNR00701.1"
                     /translation="MSPKEDEAPTAVTDVAEAPAPRKRGRPRKNPEAAPAAPKQAAAK
                     PATAKPAAAVQVAEDEPAVAAQTEPEVAVPSERQRAKPVERASDEPRRGGMGRQADGR
                     RQSEGRQADGRQSDDRQTDDRRADSLQDDADDAAEGDDSAPGGDDGQRGRGRNRRNRA
                     ARKPGYNFRELHSKILPELHLLAKEVQLEDYRRMSKDDLVVAILERSA"
BASE COUNT          219 a          384 c          396 g          152 t
ORIGIN      
        1 tccgtctacc acctgcaacg cggctagcgc ggcttgggcg caggcttcgg ggagcggctc
       61 ggggaggata ccttacgcgc cggccggcct ccgggctctc accgttcccg gatcgctgtg
      121 ggtggcacca ggcgcgagga ctgttcccgt catcgcctta ctccagggta gtgacgtaga
      181 tcacgccctg gaagcgcgcg acactttaca accagcagtg tcggcgctag actcaaacgt
      241 agcagcaggc atgaggcacg tcaataggtt ctgttgctgc ccctaagcac aaccaggcgc
      301 ttcctggtaa ggcctcgagc catcaacgga cctgagtcga cttatgcgac cgttcgcctc
      361 cacggcgcta aggcgcggcc cctcgcaagg ccatccgcct gcgcgcgatc ttggcgggcg
      421 ggttctgagg acagaagcgt tctgaccaag cagtcactcg tgtggagaaa ccgaatatgt
      481 cggctagaac gcaagccgta tagccggaag ctgtcgaagg agatgggatg agcccgaaag
      541 aagacgaagc gcctaccgcc gtgacggacg tagcggaagc gccggcgcca cgcaagcgcg
      601 gtcggccacg caagaacccg gaggccgcgc cggcggcgcc caagcaggcc gccgccaagc
      661 cggcgaccgc caagccggca gccgctgtgc aggtggctga ggacgagccc gcggtcgctg
      721 cccagaccga gccggaagtg gcggtcccga gcgagcgtca gcgggcgaag cccgtcgagc
      781 gcgcgtccga tgagccgcgg cgtggcggca tgggccgtca ggccgacggt cgtcgtcagt
      841 cagaaggtcg ccaggcagac ggtcgtcagt ccgatgaccg tcagacagac gaccgcaggg
      901 ccgactcgct ccaggacgat gctgacgacg cggcggaggg cgacgactcg gcgccgggag
      961 gcgacgacgg ccagcgcggc cgcgggcgca accggcgcaa ccgcgccgcc cgcaagcctg
     1021 gttacaactt ccgcgagttg cactcgaaga tcctgcccga actgcacctg ctcgccaagg
     1081 aagtacagct cgaggactac cgccgcatga gcaaggacga tctggtggtc gccatcctcg
     1141 agcgctccgc g
//
LOCUS       DAOQYE010000874         1150 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_166097_length_1150_cov_3.168950, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000874 DAOQYE010000000
VERSION     DAOQYE010000874.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1150
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_166097_length_1150_cov_3.168950"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            388..876
                     /locus_tag="PKN52_11910"
     CDS             388..876
                     /locus_tag="PKN52_11910"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNR00702.1"
                     /translation="MHQPATGTPVVTPHIIELNDVSKEFGGVTVLDAVSMQVPAGAYL
                     ALGGVSGAGKTTRAERKTRSLTLLNELGLEHRSHHRAIQLAGGEQQRVAIARALINDP
                     EVILADEPTGNLPHELWSHVLDLLEAEWKNGKTVIVITHEPGVAARAQRVVRLRDGRL
                     EG"
BASE COUNT          191 a          374 c          399 g          186 t
ORIGIN      
        1 tggacacccc gaagaacccg ctcgaccacg ccttgatgct gtagagctcg tcggcgtccg
       61 tgaggctgaa acctgggtcg agggtgggtc tgctcacttc tggtcctcca cgtggcgggg
      121 cgctccgccg tgctgcttcg agccgcgcgg ccgagtggcc ggcacggctc gcggttcggt
      181 gggaacgcga gcgccgcttc ttggagcgcg tgcctcgccg aacggattac gctagcactt
      241 cctcgggaaa ggttggggcc acgcgggccg gaccacctgg cgcggctgga gggccagctc
      301 cgccttcatc gtcgacgttg ctcgtgcgca tggagcgcgt cgcggagaag ggcctggcgt
      361 catctgccgg cctctagcat gttcgccttg caccaaccgg cgaccggcac ccccgtcgtg
      421 acaccgcaca tcatcgagtt gaacgacgtc tccaaggagt tcggtggcgt tacggtgctc
      481 gacgccgtga gcatgcaggt gcccgctggg gcctacttgg cactaggtgg ggtgtcaggt
      541 gccggcaaga cgacccgcgc ggagcgcaag acgcgtagcc tcaccctgct gaacgaactg
      601 gggctcgagc accgtagcca ccatcgcgcc atacagctcg ccgggggcga gcagcaaagg
      661 gtagcgatag cccgagcgct catcaacgac ccggaagtga tcctcgccga tgagccgaca
      721 ggcaacctgc cgcatgagct gtggagtcat gtcctcgacc tgctcgaagc cgagtggaag
      781 aacggcaaga ccgtgatagt catcactcac gagccaggag tagcggccag ggcccagcgg
      841 gtagtgcgcc tgcgggacgg tcggctggag ggctagcact gtggctcgga taggtgattg
      901 cagagccccg ttgccgggtg ggccagggat gatcgcgctg ccggacctcg agttccgcga
      961 cctcgacccg gagccggttc gccggcaggg cttcgctggc aagaccgggt cgccatgcac
     1021 ctacgcctgg agcccctgtc tgctttcgat gccgccgagt tcgtacccgg ttggacccta
     1081 cgagacaaag cgaccggcta cggcatcctc ggagggaccc ccgcctattg ggccgcgatg
     1141 gacccgaagg
//
LOCUS       DAOQYE010000875         1150 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_166109_length_1150_cov_2.974429, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000875 DAOQYE010000000
VERSION     DAOQYE010000875.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1150
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_166109_length_1150_cov_2.974429"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /locus_tag="PKN52_11915"
     CDS             complement(<1..619)
                     /locus_tag="PKN52_11915"
                     /inference="COORDINATES: protein motif:HMM:NF015549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HNR00703.1"
                     /translation="MAALYPLALRLALVSSPAQRPRFGGGLAASLPIALAYFPVAVSF
                     GVAGAKAGFSLAETAFMSVVIYAGASQFLALALLAGGAAPLLAVVSLLAMNARHLLYA
                     PALLNRVRAVAGAGEHGVAPGRDGGGARRRLRWSWLWAHGLTDEVFASALGRLAANRG
                     GGWSEAWQVGIGMGAYLAWVSGTVVGAVLGGGAFERWPAVDAALAF"
     gene            618..1085
                     /locus_tag="PKN52_11920"
     CDS             618..1085
                     /locus_tag="PKN52_11920"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNR00704.1"
                     /translation="MDEPVFLTASAMLEHWLGHRRLTRRVIEAFPEDQLYTYSLGGMR
                     TFAELAGEMITMAVPTVEGAVTREWPTYKVELPKTKAGLLEKWDADSKRIAELWPQIP
                     PERFEEEDVAFGMFPGKVNWLLHYIIDNEIHHRGQGYVYLRALGVEPPAFYER"
BASE COUNT          205 a          422 c          380 g          143 t
ORIGIN      
        1 ggaaggcgag cgccgcgtcc acggccggcc aacgctcgaa cgccccgccg ccaagcacgg
       61 cgccgacgac ggtccccgat acccaggcca ggtaggcccc catcccgatc ccgacctgcc
      121 acgcctcgct ccagccgccg cccctgttgg ccgccaaccg ccccaaggcg gaggcgaaca
      181 cctcgtccgt caggccgtgc gcccagagcc acgaccagcg cagccgccgc cgcgccccac
      241 caccgtcgcg gcctggcgcc acgccgtgct cgcccgcgcc cgccacggcg cgtacccggt
      301 tgagcaaggc gggcgcgtag agcaagtgac gcgcgttcat ggccagcagc gacacgacgg
      361 cgaggagcgg cgcggcgccg ccggcgagca gcgcgagcgc gaggaactgg ctcgcgcccg
      421 cgtagatgac gacggacatg aacgcggtct cggcgagcga gaagccggcc ttggcgccgg
      481 ccaccccgaa cgacaccgcc acggggaagt acgcaagggc gatgggcagg ctggcggcga
      541 ggccgccgcc gaaccgcggt cgctgcgctg gtgagctcac gagcgccaag cgtagcgcga
      601 gcggatagag tgctgccatg gacgaacccg ttttcctgac cgcgagcgcg atgctggagc
      661 attggctagg ccaccgcagg ctgacccgcc gcgtcatcga ggccttcccg gaggaccagc
      721 tctacaccta ctcgctaggc ggcatgcgca cgttcgccga actggcaggc gagatgatca
      781 ccatggcagt gccaacggtc gagggtgcgg tcacgcgcga atggcctacc tacaaggtcg
      841 agctgcccaa gacgaaggcg ggcctcctag agaagtggga cgcggactcg aagcgcatcg
      901 cagagctgtg gccccagatc ccgccggaac gcttcgagga ggaagacgtc gccttcggca
      961 tgttccccgg gaaggtgaac tggcttcttc actacatcat cgacaacgag atccaccatc
     1021 gcggccaggg ctacgtctac ctacgcgccc tcggcgtcga gcccccggcc ttctacgaac
     1081 gttgagaaag ccccggctcc tgtagcatct tcgggtgctg cggcgtttct tctcgtacta
     1141 cgccccgtat
//
LOCUS       DAOQYE010000876         1149 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_166484_length_1149_cov_1.991773, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000876 DAOQYE010000000
VERSION     DAOQYE010000876.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1149
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_166484_length_1149_cov_1.991773"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="PKN52_11925"
     CDS             <1..292
                     /locus_tag="PKN52_11925"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNR00705.1"
                     /translation="NLVSNAGRYARSEVRLEVFNNMVRVQDDGPGLPAPLEQLAQPFV
                     GRTVEIAGKEYAGGTGGLGLFIARRVLEAHGGRLVCEASGPNGTVLLAYFGQ"
     gene            305..928
                     /locus_tag="PKN52_11930"
     CDS             305..928
                     /locus_tag="PKN52_11930"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNR00706.1"
                     /translation="MTSDDQRAAPAPVRVLIADDHPLFRMGLSLALKADGFEVVAEAE
                     NGRQAVERARQLDVDVVLLDVRMPELDGIAACRAIAATADAPVVVMLTTFEEPAVIEA
                     ARAAGARAYLSKETSPRELARMLHNILAAPERDWLPEVALPELTPRESQALALMARGL
                     SNKAIASELGISPDTVKDYLENVYRKLDVRDRVSAVRRAAELGLVDR"
BASE COUNT          169 a          403 c          421 g          156 t
ORIGIN      
        1 caacctcgtg agcaacgctg ggcggtacgc ccgctccgag gtccgcctcg aggtcttcaa
       61 caacatggtg cgcgtccagg acgacggccc ggggctgccg gcgccgctgg agcagctcgc
      121 tcagccgttc gtggggcgca ccgtcgagat cgcaggcaag gagtacgcgg gcggcacggg
      181 tgggctcggg ctcttcatcg cgaggcgggt cctcgaagcg cacggcggta ggctcgtgtg
      241 cgaggcgagc ggccccaacg ggaccgtgct cctcgcgtac ttcggccagt gagggaggag
      301 cggcatgacg tctgacgatc agcgagcggc cccggcgccc gtgagggtgc tgatcgctga
      361 cgaccacccg ctcttccgca tggggctgtc gctcgcgctc aaggcggacg gtttcgaggt
      421 ggtggccgag gccgagaacg gcaggcaggc cgtggagagg gcgcggcaac tcgacgtcga
      481 cgtagtcctc ctcgacgtca ggatgccgga gctggacggc atcgcggcct gccgcgcgat
      541 agccgcgaca gccgacgcgc ccgtcgtcgt catgctgacc accttcgagg agcccgccgt
      601 gatcgaggcc gccagagcgg ccggcgcgcg cgcctacctc tccaaggaga cgagcccgcg
      661 cgagctggcc cggatgctcc acaacatcct cgccgccccg gagcgcgact ggcttcccga
      721 ggtcgccctg cccgagctga cgccgcgcga gtcgcaagcg ttggcgctca tggcgcgcgg
      781 tctgagcaac aaggcgatcg cctcggagct cggcatcagc cccgacaccg tcaaggacta
      841 cctcgagaac gtctaccgca agctcgacgt gcgcgaccgc gtgagcgccg tcaggcgcgc
      901 cgccgagctc gggctggtgg accgctgagc tgcctgtggc gcggcggtga ccgcggggac
      961 caagacaccc ggttcgaccg cctgacctag ggcacctggg cgccggccta gctcagcgcc
     1021 gatgctgggc ggtcgtgggc ctagcgtggc cacatgagcc ccgcgcgacc ccgtcgccgc
     1081 atgcgccgtc ccggcatgaa cggggcgacc ttggccgaac tcctggtagt gctggccgtg
     1141 ctagggctg
//
LOCUS       DAOQYE010000877         1148 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_166619_length_1148_cov_2.978957, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000877 DAOQYE010000000
VERSION     DAOQYE010000877.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_166619_length_1148_cov_2.978957"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="PKN52_11935"
     CDS             <1..262
                     /locus_tag="PKN52_11935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00707.1"
                     /translation="AETAGLPRLEGWGPVRVEEALVLLRLLRPFGVLVISYSPEGFDI
                     LMDGTQLFTPSAEALREHWAAFASHRGGRIAVYPWGVSKAHE"
     gene            255..>1148
                     /gene="ftsA"
                     /locus_tag="PKN52_11940"
     CDS             255..>1148
                     /gene="ftsA"
                     /locus_tag="PKN52_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HNR00708.1"
                     /translation="MSDERIIVGLDIGTTKICTVIGEVASDGVLDIIGEGTVPSDGLR
                     KGVVVNLERTIAAVRQSVAAAQRVAGVEVHSAWVGVAGSHLKALTSHGMTAIRRGYDV
                     TRTDIDRTIENARAVPLEANMEILHVIPQEYVVDGNDGIKDPLGMSGVRLEVDVHIVA
                     GAQGPLQNLKRCATDAGVEVDGLVVQALASGLAVLNRGEQELTTLLIDIGGGTTDIGV
                     FRRGTLAHSAVIPLGGDHITQDISQLLRIPPDEAERVKRRYGVALPELADRDVELEVA
                     NPSYTASISTFELAQVIRPRVV"
BASE COUNT          191 a          382 c          406 g          169 t
ORIGIN      
        1 ggcggagacg gcggggctgc cgaggctcga aggctggggg ccagtgcgcg tggaagaggc
       61 gctggtgctc ctgcggctgc tgcggccgtt cggcgtgctg gtgataagct actcgccgga
      121 aggattcgac atccttatgg atggcacgca gttgttcacg ccgagcgcag aggctctgcg
      181 cgagcattgg gcggcattcg caagccaccg tgggggccgc atcgcggttt acccttgggg
      241 agtgagcaag gcacatgagt gacgaacgca taatcgtcgg gctcgacata ggcacgacca
      301 agatctgcac cgtcatcggt gaggtcgcct cggacggcgt tctcgacatc atcggcgagg
      361 gcacggtgcc atccgacggc ctgcgtaagg gcgtcgtcgt gaacctcgag cgcaccatcg
      421 cggccgtgag gcagtcggtc gccgccgctc agcgggtcgc gggcgtcgag gtgcactccg
      481 cgtgggtggg ggtggccggc agccacctca aggcgcttac gagccacggc atgacggcca
      541 tccgccgcgg ctacgacgtg accaggaccg acatcgaccg caccatcgag aacgctcggg
      601 cggtgccgct cgaggcgaac atggagatcc tccacgtcat cccccaggag tacgtggtgg
      661 acggcaacga cggcatcaag gaccccctcg gcatgtccgg ggtgcgcctc gaggtcgacg
      721 tgcacatcgt ggcgggggcg cagggtcctc tgcagaacct gaagcgctgc gcgaccgacg
      781 ccggggtcga ggtcgacggc ctcgtagtcc aggcgctggc gtccggcctc gccgtcctca
      841 accgcggcga gcaggagctg accacgctcc tcatcgatat cggcggcggg acaaccgaca
      901 tcggcgtctt caggcgcggc accctcgcgc actccgccgt catccccctc gggggcgacc
      961 acatcacgca ggacatcagt caactcctgc gcatcccgcc cgacgaggcg gagcgcgtga
     1021 agcgccgcta cggcgtcgcg ctgccggagc tggccgaccg cgacgtggag ctggaagtcg
     1081 ccaacccgag ctacacggcc agcatctcca ctttcgagct ggcgcaggtc atccgtccga
     1141 gggtcgtc
//
LOCUS       DAOQYE010000878         1148 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_166658_length_1148_cov_2.619396, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000878 DAOQYE010000000
VERSION     DAOQYE010000878.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_166658_length_1148_cov_2.619396"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="PKN52_11945"
     CDS             complement(<1..300)
                     /locus_tag="PKN52_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00709.1"
                     /translation="MPSPRRESLTARRERAQRVLNGLRTLYPDARTELDFRTPFELLV
                     ATMLSAQATDVSVNLATPALFAAYPDAPSMAKASAQDIEPYIKTIGLFRTKAKNVV"
     gene            471..>1148
                     /locus_tag="PKN52_11950"
     CDS             471..>1148
                     /locus_tag="PKN52_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNR00710.1"
                     /translation="MPIFEYKVRDRGGKVITGTTEALSQRDVATALRQKGYFVTEIKA
                     PKTGLQSDLKLPKWLDFGSIPNVRDITIFSRQFATVINAGLPVVQSLAILQRQAPKQG
                     LKDALKQVREDVETGQQLSNALARHPRLFNKLYIYLVRAGEVSGNLDGILDRIAAYME
                     KQAALRGKIKTALTYPTVVLVIALAVTWFLLTGIVPQFAQILDQLGGDMPVITKALIA
                     VSDFLRYQ"
BASE COUNT          200 a          391 c          370 g          187 t
ORIGIN      
        1 cacgacgttc ttggccttcg tgcggaagag gccgatcgtc ttgatgtacg gctcgatgtc
       61 ctgcgcgctg gccttggcca tgctcggtgc gtccgggtag gcggcgaaca gggccggcgt
      121 ggcgaggttg acgctcacgt cggtggcttg cgccgacagc atggtcgcga cgaggagctc
      181 gaacggcgtg cggaagtcga gttccgtgcg cgcgtcgggg tagagggtgc gcaagccgtt
      241 cagcacgcgc tgggcgcgtt cgcggcgcgc ggtcagggat tctcgcctcg ggctcggcac
      301 ggcccgcatg ctaaccccgc gccgacggga acccggccag gaagtgacct atccaccagc
      361 ttgcgcacgc cacggtcgag tacgcttgca tgcggtctag gtccgttccc gaggtcgtcg
      421 tagcggcacc gaccatggac ccgtactggt gcacagggag gcagggcagg atgccgattt
      481 tcgagtacaa ggtccgtgac cggggaggca aagtcatcac gggcaccacc gaggcgctct
      541 cccagcgcga cgtggcaacc gcgttgcgcc agaagggcta cttcgtaacg gagatcaagg
      601 ccccgaagac cggcctccaa tccgacctca agctgcccaa atggctcgat ttcggaagca
      661 tcccgaacgt ccgcgacatc acgatcttct cccgccagtt cgccactgtc atcaacgccg
      721 gtctcccggt ggtccagagc ctcgccatcc tccagcgcca agccccgaag cagggcctga
      781 aggacgcgct caagcaggtg cgcgaggacg tcgagaccgg ccagcagctc tcgaacgcac
      841 tggcgaggca ccccaggctc ttcaacaagc tgtacatcta cctcgtgcgc gccggtgagg
      901 tgtcgggcaa cctcgacggc atcctggacc gcatcgccgc gtacatggag aagcaggccg
      961 ccctgcgcgg caagatcaag acggcgctga cgtacccgac ggtcgttctc gtcatcgccc
     1021 tggcggtcac ctggttcctg ctcacgggca tcgtcccgca gttcgcacag atcctcgacc
     1081 agctcggcgg ggacatgccc gtcatcacca aggcgctcat cgccgtctcc gacttcctgc
     1141 gctaccag
//
LOCUS       DAOQYE010000879         1148 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_166730_length_1148_cov_2.116194, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000879 DAOQYE010000000
VERSION     DAOQYE010000879.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_166730_length_1148_cov_2.116194"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1148
                     /locus_tag="PKN52_11955"
     CDS             <1..>1148
                     /locus_tag="PKN52_11955"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNR00711.1"
                     /translation="APVAGPVPGGLGLTGAGVRAPALEGSYASYITAVLYAAGGLVTD
                     AATVLENVPTADLSEHALLFKQDLAAVTGAAADDSQPTDLGTARLLRRAVLRLGLPTF
                     DDAATADAFAAVAAATDLPLANAWTAVMAVSAGDIESARAPLAAAEAGGYAYATALAA
                     SLALATGDLDAYAAGVGRTIAGGAHDGSAALLGASVTAQMAQDTQSEKAALHELGRAA
                     PFLAYPFERLSFIAFDEADAGAAAEALAVAVQLDPESDLYWTNLGWSYYLLGFLERSE
                     AASLRAVELDANQFIANYNIGLVRSVTGRLDEALDAYDRALRLDPSVDDEAIKDLENA
                     RSLYPGAVAVHYSLGYLYDAEGRRADARTSLRTFVRRASAAGLYPEFV"
BASE COUNT          157 a          447 c          383 g          161 t
ORIGIN      
        1 ccgcgccggt cgctggtccc gttccaggcg ggctgggcct gacaggcgcc ggcgtcaggg
       61 cgcccgccct ggaaggttcg tacgccagct acatcacggc cgtcctctac gcggccggcg
      121 ggctggtgac cgacgctgcc accgtgctcg agaacgtgcc cacggccgac ctctcggagc
      181 acgccctgct cttcaagcaa gacctcgccg ccgtgacggg ggcggcggca gacgactcgc
      241 agccgaccga cctcggcacg gcgcggctgc tgcgccgtgc cgtgctcagg ctcggactcc
      301 cgaccttcga cgacgccgcg acggccgacg ccttcgccgc cgtcgcagcg gctaccgacc
      361 tgcccttggc caacgcttgg accgccgtca tggccgtcag cgccggcgac atcgagagcg
      421 cacgcgcgcc gctcgccgcc gcggaggcgg ggggctacgc ctacgccacg gcgctcgcgg
      481 cctcgctagc cttggcgacc ggcgatctgg acgcctacgc cgccggcgtg ggacgaacga
      541 tcgccggtgg agcgcatgac ggcagcgcgg cgctcctcgg cgcgagcgtc accgcccaga
      601 tggcgcagga cacgcagtcg gagaaggcgg cgttgcacga actgggtcgc gccgccccgt
      661 tcctcgcgta ccccttcgag cgcctgtcgt tcatcgcgtt cgacgaggcg gatgccggtg
      721 ccgctgccga ggcgctcgcc gtggccgtgc agctcgaccc ggagtccgac ctctactgga
      781 cgaacctcgg ctggtcctac tacctcctcg gcttccttga gcgtagcgag gccgcctcgc
      841 tacgcgccgt cgaactcgac gccaaccagt tcatcgccaa ctacaacatc ggcctcgtgc
      901 gctcagtcac cggccgcctg gacgaggccc tcgacgcgta cgaccgcgcg ctgcgcctcg
      961 atcccagcgt ggacgacgag gccatcaagg acctcgagaa cgccaggagc ctctacccgg
     1021 gcgccgtcgc cgtccactac tcgctcggct acctctacga cgctgaaggc cgcagggccg
     1081 atgcgcgcac gtcgctgcgc acgttcgtga ggcgcgcgag cgctgccggt ctctacccgg
     1141 agttcgtc
//
LOCUS       DAOQYE010000880         1144 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_167725_length_1144_cov_2.078972, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000880 DAOQYE010000000
VERSION     DAOQYE010000880.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_167725_length_1144_cov_2.078972"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..853
                     /locus_tag="PKN52_11960"
     CDS             <1..853
                     /locus_tag="PKN52_11960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00712.1"
                     /translation="HGLSFAEPEVSENLALRFARDPGFAGFQVGLLHANVAGLGGHDN
                     YAPTTMADLHAAGLDYWALGHVHTRAVLQERDPVVVYPGNLQARHAGERGERGFYLVH
                     VEDGRATLEFVDVGLVRFDDVTIDIAGLADVDAVHAALAAAVGPAGAAHTHVLRAELR
                     GAGPANAALRAGGEQGLTDYLRDTAPPNVWWEEVRLSTRPPMDRAARLAAGDFVADLL
                     RRSDAGRPEAARAVAALARQLNDHPHLRPVAALLDLASFEADAGAIWDEAEALALDLL
                     DVGYREA"
     gene            857..>1144
                     /locus_tag="PKN52_11965"
     CDS             857..>1144
                     /locus_tag="PKN52_11965"
                     /inference="COORDINATES: protein motif:HMM:NF024904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNR00713.1"
                     /translation="MRLGRVSIGRFRALEGTELELGPGLVVVEGPNEAGKSTLLAFIQ
                     ALLFPTALAAATRVEGSLDLHDADRAYRVIRSGARATHRLLDLASGDQLDLS"
BASE COUNT          144 a          438 c          410 g          152 t
ORIGIN      
        1 gcacggcctc tccttcgctg aaccggaggt cagcgagaac ctcgcgctgc gcttcgcgcg
       61 cgacccgggc ttcgcggggt tccaggtcgg cctgctgcac gccaacgtcg ctgggttggg
      121 cgggcacgac aactacgcgc cgacgaccat ggccgacctg cacgccgccg gcctcgacta
      181 ctgggcgttg gggcacgtcc acacgcgcgc cgtcctgcag gagcgggacc ccgtggtcgt
      241 ctaccctggc aacctgcagg cccgtcacgc cggcgagcgc ggcgaacgcg gcttctacct
      301 ggtgcacgtc gaggacggac gcgcgacgct cgagttcgtc gacgtggggt tggtgcgctt
      361 cgatgacgtc acgatagaca tcgccggcct ggccgatgtc gacgcggtgc acgccgcgct
      421 ggccgcggcc gtgggtccgg cgggcgccgc gcacactcac gtcctgcgcg ccgagctgcg
      481 cggtgccgga cccgccaacg ccgcgctccg cgccggcggg gagcaagggc tcacagacta
      541 ccttcgcgac acggcgcccc ccaacgtctg gtgggaggag gtgcgcctca gcaccaggcc
      601 gcccatggac cgcgcggctc gcctcgccgc cggcgacttc gtggcggacc tgctgcgacg
      661 cagcgacgcg ggccggccgg aggccgcgcg agcggtcgcg gcgctcgccc ggcaactgaa
      721 cgatcacccg cacctgcgcc ccgtcgcggc gctcctcgac ctggcctcgt tcgaggcgga
      781 cgccggggcc atctgggacg aggccgaggc cctcgctctc gacctgctgg acgtcggcta
      841 ccgggaggcc tgagccgtgc gactgggccg cgtctccatc ggtcgcttcc gcgcgttaga
      901 gggcacggag ctcgagctcg gaccgggcct ggtggtcgtg gagggcccga acgaggcggg
      961 caagagcacc ctgttggcct tcatccaggc gctcctcttc cccacggcgc tcgcggccgc
     1021 cacccgggtg gaggggagcc tggacctcca cgacgccgac cgcgcctacc gcgtgatccg
     1081 cagcggcgcg cgcgccaccc accgcctcct ggacctcgcg agcggcgacc agctcgactt
     1141 gtct
//
LOCUS       DAOQYE010000881         1142 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_168145_length_1142_cov_2.569457, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000881 DAOQYE010000000
VERSION     DAOQYE010000881.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_168145_length_1142_cov_2.569457"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..818)
                     /locus_tag="PKN52_11970"
     CDS             complement(<1..818)
                     /locus_tag="PKN52_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNR00714.1"
                     /translation="MRPPDDLPGIRSVPLISVRDLTKRFPGVVANDSVSLDLLPGEVH
                     ALLGENGAGKTTLISMLYGLLQPDGGSIAMDGRRVAIKNPRHAMDLGIGLVAQHFNLA
                     RRHTVAENLAVGLRGTPFWRPTRALNKRAAELTERYGLAVRMSAQVASLSPGERQRVE
                     ILKAVMQGARLLILDEPTSVLTPQEADALLKVVSDMRRDGRGILFISDKLGEVLSVAD
                     RITVLRGGKVVGRRARGAADASELARLMVGAPTATPQRLGIPPEGEPVLRVAGV"
BASE COUNT          157 a          393 c          417 g          175 t
ORIGIN      
        1 cacacgcccg cgacgcggag gacgggctcg ccctcgggag gaatgccgag ccgctgcggg
       61 gtggcggtcg gcgcgccgac catgaggcgc gccagctcgc tcgcgtcggc cgcgccgcgc
      121 gcgcggcgcc cgacgacctt gccgccccta aggacggtga tgcgatcggc gacgctgagc
      181 acctcgccga gcttgtcgct gatgaaaagg atgccccggc cgtccctgcg catgtccgag
      241 acgaccttca ggagcgcgtc ggcctcctgg ggcgtcagca cgctcgtcgg ctcgtcgagg
      301 atgaggaggc gcgcgccctg catgacggcc ttgaggatct cgacccgttg gcgctccccg
      361 ggggagaggc tcgcgacctg ggcgctcatg cggaccgcga gaccgtaacg ttccgtgagc
      421 tcggccgcgc gcttgttcag tgccctggtc gggcgccaga agggcgtgcc gcgcaggccc
      481 acggcgaggt tctccgcgac cgtgtgccgc ctggcgaggt tgaaatgctg ggccacgagg
      541 cctatgccga ggtccatggc gtgcctcggg ttcttgatgg cgacgcgccg gccatccatg
      601 gcgatgctgc cgccgtcggg ctggagtagc ccgtagagca tgctgatcag cgtggtcttg
      661 ccggcgccgt tctcgcccaa cagggcgtgc acctcacctg gaagcaggtc gaggctcacg
      721 gaatcgttcg ctaccactcc tgggaagcgc ttggtgaggt cgcgaacgct gatcagcggg
      781 acggaacgaa tacccggcag gtcgtcgggc ggtcgcacct aggtaaggta acacaagggg
      841 cgctaggcct ggtgcgggca cccgcggggc gcgacccaag cccagccgga cggaacctgc
      901 ccctcgaccc gcctaccggc cggcggtccg actaacctcc agcggcgcta ccctctaggg
      961 gcgcctaggg gcgctcgctt cccgggccgg ctcaggtaag gggggactcc gcccggcgcg
     1021 tgtcgcgctc gtagtacggg ttctcgcccg tcgcgtggtc cgtggcatcc atgaccccgc
     1081 ggatctcggg cacctgctcc ttgaggatcc ggtcgatgcc ctgcttgagc gtgaggtcgg
     1141 cg
//
LOCUS       DAOQYE010000882         1139 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_168937_length_1139_cov_2.740775, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000882 DAOQYE010000000
VERSION     DAOQYE010000882.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1139
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_168937_length_1139_cov_2.740775"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="PKN52_11975"
     CDS             complement(<1..278)
                     /locus_tag="PKN52_11975"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNR00715.1"
                     /translation="MKRVLIALFVACLAFVTAVASAQDTVKIGVITSLTGRFAEFGEQ
                     HKAGIQAALEDVNAAGGVNGALVEVVFEDDISEVNAALTAGEKLVNQGL"
     gene            540..>1139
                     /locus_tag="PKN52_11980"
     CDS             540..>1139
                     /locus_tag="PKN52_11980"
                     /inference="COORDINATES: protein motif:HMM:NF037997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015114 - phosphate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi symporter"
                     /protein_id="HNR00716.1"
                     /translation="MLLTMLGGLALLLTGTYRISSALQEAIGPASRRWLALATKSPVM
                     ALLTGTAVAAATQSGTSISVTILSLLGNGFVAVREGIVMLMGAKLGATLAMQLAAFHL
                     SDYALPMIGVGYVLSLWRRGRSIGEFVLGTGLLFFGLELTVQSVSGLSSSDVFNVLIQ
                     AAEQQPLAVLTLGGALGTMLSSSNAATAVALGLHVAGAVS"
BASE COUNT          152 a          393 c          379 g          215 t
ORIGIN      
        1 agtccttggt tgacgagctt ctcgccggcg gtgagggcgg cgttcacctc ggagatgtcg
       61 tcctcgaaca ctacttcgac gagggcgccg ttgacgccgc cggccgcgtt gacgtcctcg
      121 agggccgcct ggatgccggc cttgtgctgc tcgccgaact cggcgaagcg gccggtgagg
      181 ctcgttatga cgccgatctt gacggtgtcc tgggcgctcg ccacggcggt cacgaacgcc
      241 aggcaggcta cgaacaaggc gatgaggact cgcttcatgc ttcctccata aggtgcagca
      301 gattgtttgt cgccctaagt tagcagccgg acgcgttccc ggtcaccgtc aggggaacgg
      361 aggcgcgtac acacgtcgtg atacacgggg ccggctggct acgccgcggc gccatcggtc
      421 gccagccggc ccttggccgg gtgtagactg ctcatccatc tccaggacgg gatgtaacgt
      481 accttgtcgc tcaccgcctg tttgtcgaag cccagtacct gcccgccgga ggggccggca
      541 tgctgctgac gatgctcggc ggtctcgcgc tgctgctgac cggcacctac cggatctcct
      601 cggcgctgca ggaggccatc ggtccggcct cgcggcgctg gctggcgttg gcgacgaagt
      661 cgccggtcat ggcgctgttg acgggcacgg ccgtagcggc cgccacccag agcggcacgt
      721 ccatctccgt caccatcctc agcctgctcg gcaacggctt cgtggcggtg cgcgagggca
      781 tagtcatgct gatgggcgcc aagctcggcg ccacgctggc catgcagctg gccgctttcc
      841 acctctccga ctacgccctg cccatgatcg gcgtcggcta cgtcctctcc ttgtggcgca
      901 ggggcaggtc gatcggcgag ttcgtgctcg gtacgggcct gctcttcttc ggcctcgaac
      961 tcacggtcca gtcggtctcc ggtctgagtt ccagcgatgt cttcaacgtc ctcatccagg
     1021 ccgcggagca gcagccgttg gcggtgctca ccctcggcgg cgcgctcggc accatgctct
     1081 ccagctccaa cgcggccacg gccgtggccc tcggcttgca cgtcgcgggg gcggtctcg
//
LOCUS       DAOQYE010000883         1135 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_169990_length_1135_cov_2.348148, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000883 DAOQYE010000000
VERSION     DAOQYE010000883.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1135
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_169990_length_1135_cov_2.348148"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1135)
                     /gene="ccsA"
                     /locus_tag="PKN52_11985"
     CDS             complement(<1..>1135)
                     /gene="ccsA"
                     /locus_tag="PKN52_11985"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HNR00717.1"
                     /translation="ARLAAVAGFILATAAVVVMQAALLTNDFSVSYVAGHSRIASPTW
                     VKVVTMWAALEGSILLWAWLLTGYTALLAITAPLSPLRPYAISIMQAVQVFFLGVLAF
                     LANPFKVVPFPPLDGPGPNELLQNHWMMAVHPLLMYLGFVGLTVPFAYAMAALITRRP
                     GTEWMTQTRRWTLTGWGFLSGAIVAGGWWSYEVLGWGGYWAWDPVENVSFMPWLTATA
                     FIHSVQVQERRRMLKPWNVLLIVATFSLSILGTFLTRSGVVSSVHAFGNGPVGPAFLV
                     FFLLVTLAAFGLLAARWDDVKDRADLDSVVSREGGFLGVNILFVTLTAAVLLGTLFPL
                     IVEAVTGDKVTVGAPFFDQTTLPLWMLVLLLMGTFILFNPELAL"
BASE COUNT          223 a          359 c          419 g          134 t
ORIGIN      
        1 caacgccagc tctgggttga acaggatgaa ggtgcccatc agcaggagca ccagcatcca
       61 cagcggcagg gtcgtctggt cgaagaacgg ggccccgacc gtgaccttgt cgcccgttac
      121 ggcctccacg atcagcggga agagcgtgcc gagcaggacg gccgccgtga gggtcacgaa
      181 gaggatgttg acgccgagga agccgccctc ccggctcacg acggagtcga ggtcggcgcg
      241 gtccttgacg tcgtcccagc gcgcggcgag gaggccgaac gcggccaacg tcaccagcag
      301 gaagaagacg aggaaggccg gcccgacggg cccgttgccg aacgcgtgga cggaggagac
      361 gacgccggag cgggtaagga acgtgccgag gatcgagagc gagaacgtgg cgacgatgag
      421 cagcacgttc cacggcttga gcatgcgccg ccgctcctgg acctgcacgc tatggatgaa
      481 cgcggtcgcg gtcaaccatg gcatgaagct cacgttctcg acggggtccc acgcccagta
      541 gccgccccag ccgaggacct cgtagctcca ccagcccccg gccacgatgg cgccggagag
      601 gaacccccag cccgtcaggg tccagcggcg cgtctgcgtc atccactcgg tgcccggccg
      661 gcgcgtgatg agcgcggcca tggcgtaggc gaacggcacg gtcagcccca cgaagcccaa
      721 gtacatgagc agcgggtgca cggccatcat ccagtggttc tggaggagct cgttcggacc
      781 ggggccgtcg agcggtggga acggcacgac cttgaacggg ttggcgagga aggcgagcac
      841 gccgaggaag aagacctgca cggcctgcat gatcgagatg gcatacgggc gcagcgggct
      901 caacggggcc gtgatggcca gcaacgccgt gtagccggtg aggagccagg cccacaggag
      961 gatggagccc tccagggcgg cccacatcgt cacgaccttc acccacgtgg gggatgcgat
     1021 gcgcgagtgt ccggccacgt aggagacgct gaagtcgttc gtgaggagcg cggcctgcat
     1081 gacgacgacg gcggccgttg cgaggatgaa ccccgcgacg gcggcgagcc gggcg
//
LOCUS       DAOQYE010000884         1134 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_170233_length_1134_cov_2.518072, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000884 DAOQYE010000000
VERSION     DAOQYE010000884.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1134
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_170233_length_1134_cov_2.518072"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1134)
                     /locus_tag="PKN52_11990"
     CDS             complement(<1..>1134)
                     /locus_tag="PKN52_11990"
                     /inference="COORDINATES: protein motif:HMM:NF012431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNR00718.1"
                     /translation="QQLEHPLEHDVSVNDTFRPVSRFFTRVTRPEQLLSVLPEAMRVL
                     TDPAETGAVTIALPEDVQTEAYDWPAEFFAERVWRVRRPEPEPELVAEVGRRLARARR
                     PLVVCGGGVIYSGASMELAAFARRYGVPVVESQAGKGALPWDHPSNVGAVGAAGGLAA
                     NRLAATADLVIAIGTRLGDFVTASKTAFQDPAVEFVSINVVPADAGKLRSLPLVADAK
                     RALACLGAELASAGWTGAPTGWRERVAALKAEWDGVVDDLRAGKGLAATRGGVDPGER
                     LALADAGVPADRLAQSEVIGIVNDVVGGHATVVCAAGSMPGDLLKLWRPEDPKAYHLE
                     YGYSCMGYEIPAGIGIKLAEPEREVVVMIGDGSYLMMNSEIVTA"
BASE COUNT          157 a          421 c          399 g          157 t
ORIGIN      
        1 cgccgtcacg atctcgctgt tcatcatcag gtaggagccg tcgccgatca tgacgacgac
       61 ctcgcgctcc ggttcggcca gcttgatgcc gatgccggcc gggatctcgt agcccatgca
      121 cgagtagccg tactccaggt gatacgcctt cggatcctcc ggccgccaca gcttcagcag
      181 gtcgccgggc atgctaccgg ccgcgcatac cacggtggcg tgaccgccga ccacgtcgtt
      241 gacgatgccg atgacctcgg actgcgccag gcggtcagcc ggcacgccgg catctgccag
      301 cgccaagcgc tcgcccgggt cgaccccacc gcgggtagcc gcgaggccct tgccggcccg
      361 caggtcgtcg acaacgccgt cccactccgc cttgagggcg gccacgcgct cccgccagcc
      421 ggtcggggcg ccggtccagc cggccgacgc cagttccgcg ccgaggcatg ctagggcgcg
      481 cttggcgtcg gcgacgagcg gcaagctccg tagtttgcca gcgtcggccg gcacgacgtt
      541 gatgctcacg aactccacgg cggggtcctg gaaggccgtc ttggaggccg ttacgaagtc
      601 gccgaggcgc gtgccgatgg cgatgacgag gtcggcggtg gcggccaggc ggttggcggc
      661 tagcccgccg gccgctccga ccgcgcccac gttgctcggg tggtcccacg gcagagcgcc
      721 cttgccggcc tgcgactcca ccaccggcac gccgtagcgc cgcgcgaacg ccgcgagctc
      781 catgctggcg cccgagtaga tcacgccgcc gccgcagacg acgaggggcc gccgcgcccg
      841 tgccaggcgc ctgccgacct cggcgacgag ctcgggctcc ggctcggggc gccgcacgcg
      901 ccacacgcgc tcggcgaaga actccgcggg ccaatcgtac gcctccgtct gcacgtcctc
      961 cgggagcgcg atcgtgaccg cgcccgtttc ggccgggtcg gtcaggacgc gcatggcctc
     1021 gggcagaacc gagaggagct gctccggccg cgtgacccgc gtgaagaagc gcgagacggg
     1081 tcggaaggtg tcgttgacgc tcacgtcgtg ttcgagcgga tgctcgagct gctg
//
LOCUS       DAOQYE010000885         1133 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_170519_length_1133_cov_2.223562, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000885 DAOQYE010000000
VERSION     DAOQYE010000885.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_170519_length_1133_cov_2.223562"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="PKN52_11995"
     CDS             <1..217
                     /locus_tag="PKN52_11995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00719.1"
                     /translation="VIEACAKTGTDTAFLDQTARTRGVDDTPYLIEIFTAISREYEAG
                     VIQGVREPAAALRRAAQQAKDIISGFF"
     gene            270..>1133
                     /locus_tag="PKN52_12000"
     CDS             270..>1133
                     /locus_tag="PKN52_12000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00720.1"
                     /translation="MRTTRLFATLLALLLATTAAAETRSFTVKGGSAAAPVPVSLDLL
                     TVRNTIGADFAFDWSSLQVLVGGAAVPHQIDDTDLNGRVSAGDELSFVAAGDATVQVS
                     DEPGEAASAAPAFEVGQDGDATVIKSLTTEGFQVTVAADGLASITGFGAVTDTLAAEL
                     GILRFSGFPESTYWANGELGPHEEYTTLEAGGMRHVRTEVLPAGPVRVTVVSEYASDR
                     FVGLNQRVVTRVFATGDVDVSNDVTFRGYSDMMKLQHMVTTVMSQADQDATHVLPVFR
                     RLLWADQLGVSI"
BASE COUNT          194 a          412 c          367 g          160 t
ORIGIN      
        1 ggtcatcgag gcctgtgcga aaacgggtac ggacaccgcc ttcctcgacc agacggcgcg
       61 gacgcgcggg gtggacgaca ccccttacct gatcgagatc ttcacggcca tcagtcgcga
      121 gtacgaggcg ggcgtcatcc agggcgtccg cgagcctgcc gctgccctgc gccgcgccgc
      181 gcagcaggcg aaggacatca tcagcgggtt cttctgagca ggttcttaag caagcaagac
      241 gcgcgagagc gcgaagaagg gaaggacgaa tgagaaccac cagactgttc gcaacgctac
      301 tggcactcct actcgccacc accgcggccg ccgagacgcg gagcttcacg gtgaaaggcg
      361 gcagcgcggc agcgcccgtg cccgtgtcgc tcgacctcct caccgtgcgc aacaccatcg
      421 gtgcagactt cgctttcgac tggagcagcc tccaggtcct ggtgggtggt gccgcggttc
      481 cccaccagat cgacgacacc gacctcaacg gtcgcgtctc ggccggcgac gagctcagct
      541 tcgtcgccgc gggcgacgcc accgtccagg tgagcgacga gcccggcgag gccgcctcgg
      601 cggcccctgc gttcgaggtt ggccaggacg gcgacgccac ggtcatcaag agcctcacca
      661 ccgagggctt ccaggtcacg gtcgccgccg acgggctcgc tagcatcacg ggcttcggcg
      721 cggttaccga cacgctggcg gccgaactcg gcatcctgcg cttcagcggc ttccccgaga
      781 gcacctactg ggcgaacggc gagctcggcc cgcacgagga gtacacgacg ctcgaggccg
      841 gcggcatgcg ccacgtccgc accgaggtcc tgcccgccgg gcccgtgcgc gtcaccgtcg
      901 tcagcgagta cgcctccgac cgcttcgtgg gcctcaacca gcgcgtcgtg acgcgcgtct
      961 tcgcgacggg cgacgtcgac gtcagcaacg acgtcacgtt ccgcggctac agcgacatga
     1021 tgaagctgca gcatatggtc accaccgtca tgagccaggc cgaccaggac gccacgcacg
     1081 tcctgcccgt cttcaggcgc ctcctctggg ccgaccagct cggcgtctcg atc
//
LOCUS       DAOQYE010000886         1130 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_171157_length_1130_cov_2.884651, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000886 DAOQYE010000000
VERSION     DAOQYE010000886.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1130
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_171157_length_1130_cov_2.884651"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1130
                     /locus_tag="PKN52_12005"
     CDS             <1..>1130
                     /locus_tag="PKN52_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNR00721.1"
                     /translation="AAKGELDAFEVNKGPILVFVGPPGVGKTSIAQSIAKALGREYVR
                     ISLGGARDESDIRGHRRTYIGAMPGRIIQGIRQAGAKNPVFLLDEVDKLGSSYQGDPS
                     SALLEVLDPAQNANFVDHYLGVPFDLSEVLFIATANYVQQIPEALLDRMEAIEFSSYI
                     EQEKLEIAKRYLVPRQLVENGLKPRQVDLTDGALARVITSYTREAGVRNLERLIGTLC
                     RKAARRIADGEAKRVKITERSLEQYLGAQRFTPESEAEDNLVGVATGMYYTPVGGDIL
                     FVETSVTPGKGGLVLTGQLGDVMKESARAALTYAKTNSERFGIKRSLLDDSEIHIHIP
                     AGATPKEGPSAGVALATSLISALTGVPIRKQVAMTGEITLRGR"
BASE COUNT          205 a          419 c          349 g          157 t
ORIGIN      
        1 gccgccaagg gcgagctcga cgcgttcgag gtcaacaagg gccccatcct cgtcttcgtc
       61 ggccctcccg gcgtcggcaa gacgtccatc gcccagtcga tcgccaaggc gctcggccgc
      121 gagtacgtgc gcatcagcct cgggggcgcg cgcgacgagt ccgacatccg cggccaccgc
      181 cgcacctaca tcggcgccat gcccggccgc atcatccagg gcatcaggca agccggcgcc
      241 aagaaccccg tgttcctcct cgacgaggtc gacaagctcg gcagctcgta ccagggcgac
      301 ccgtcctcgg cgctgctcga ggtcctcgac ccggcccaga acgccaactt cgtcgaccac
      361 tacctcggcg taccgttcga cctctccgag gtgctcttca tcgccacggc caactacgtc
      421 cagcagatcc cagaggcgct cctcgaccgc atggaggcca tcgagttctc ctcctacatc
      481 gagcaggaga agctcgagat cgccaagcgc tacctggtgc cgaggcagct cgtcgagaac
      541 ggcctcaagc ccaggcaggt agaccttacg gacggggcgc tggcgcgggt catcacgtcc
      601 tacacgcgcg aagccggcgt acgcaacctg gagaggctca taggtaccct ctgccgcaag
      661 gcggccaggc ggatcgccga cggcgaggcc aagcgcgtca agatcacgga gcgcagcctc
      721 gagcagtacc tcggcgcgca acgcttcacg cctgaatccg aggcggagga caacctcgtc
      781 ggcgtggcca ccggcatgta ctacacgccg gtcggcggcg acatcctgtt cgtcgagacc
      841 tcggtcacac cgggcaaggg cgggctcgtg ctgaccggtc aactcggcga cgtcatgaag
      901 gagtcggcgc gcgccgccct cacctacgcc aagacgaact ccgagcgctt cggcatcaag
      961 cgctcgctct tggatgactc tgagatccac atccacatcc ccgccggcgc cacgccgaaa
     1021 gagggcccga gcgcgggcgt cgcgctcgcc acgtcgctca tcagcgcgct cacgggcgtg
     1081 ccgatccgca agcaagtcgc catgacgggc gagatcactc tgcgtggccg
//
LOCUS       DAOQYE010000887         1130 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_171160_length_1130_cov_2.871628, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000887 DAOQYE010000000
VERSION     DAOQYE010000887.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1130
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_171160_length_1130_cov_2.871628"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1096
                     /locus_tag="PKN52_12010"
     CDS             <1..1096
                     /locus_tag="PKN52_12010"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HNR00722.1"
                     /translation="LLDTQLEEAGVDAILVTSPFQLEELSGFPSTWLAQAGASLLFVR
                     GDERLHLLLPGYGRLPGTVERATYRDVAGAVAAIAPGTLAVEAGHADLRLARLLGADP
                     SLIANASPLLYRWQELRAASQLPYYVLAANSARLATERAVAYAAGRLAQGGELRETEL
                     ALAFDTFLGEFAGECGLHGMFRPYFRIIHPGERALVPAAPSPNVVSSRNRTVKFDMGT
                     QVLDLAGRVRGCSDIAKTLCNTPELEEFQSLLRTILLDRVIPAIRPSVTGAAVHGSAT
                     EALAEQDDRARGWNLLPQGRSGKEYGRDCGHVINRQTICTIYLEPGCEQQIEAGMSGC
                     VEFVWPVDEAVVAIEDAYLVTEAGAIPITA"
BASE COUNT          172 a          401 c          389 g          168 t
ORIGIN      
        1 cctgctggac acgcaactcg aggaggccgg cgtcgacgcc atcctggtca cctccccttt
       61 ccagctggag gagctgagcg gcttcccgag cacgtggttg gcgcaggcgg gcgcgtcact
      121 gctgttcgtc cgtggtgacg agagactgca tctcctgctg cccgggtacg gtcggctgcc
      181 ggggacggtg gaacgcgcga cctaccgcga tgtggccggc gcagtcgcgg ccatcgcgcc
      241 gggcacgctc gccgtcgaag ctggccacgc cgacctgcgg ctggcgcgcc tcctcggtgc
      301 cgacccgtcg ctgatagcca acgcctcgcc gctgctctac cgctggcagg agctgcgagc
      361 cgccagccaa cttccctact acgtcctggc cgccaactcg gcccgcctgg ccaccgaacg
      421 cgccgtcgcg tacgccgccg ggaggctggc gcaaggcggt gagctgcgcg aaacggagct
      481 ggcgctcgcc ttcgacacct tcctcgggga gttcgccgga gagtgcggac tgcacggcat
      541 gttccgtccc tacttccgca tcatccatcc cggcgagagg gccctggtcc ctgccgcgcc
      601 gagccccaac gtcgtgagta gccggaaccg caccgtcaag ttcgatatgg gaacgcaggt
      661 cctcgatctg gccgggcgcg tgaggggctg ctccgacatc gccaagacgc tgtgcaacac
      721 gccggagctc gaggagttcc agtcgctcct ccgaaccatc ctgctcgatc gcgtcatccc
      781 cgccatccgt cccagtgtga ccggcgccgc cgtccacggc tcggccaccg aggcgctggc
      841 cgagcaagac gacagggcgc gcggctggaa cctgttgccg cagggccgca gcggcaagga
      901 gtacggcaga gactgcggtc acgtcatcaa ccggcagacg atctgcacga tctacctcga
      961 acccggctgc gagcagcaga tcgaggcggg catgtccggc tgcgtcgagt tcgtctggcc
     1021 ggtggacgag gcggtggtcg cgatcgagga cgcctatctg gtgaccgagg cgggggccat
     1081 ccccataaca gcgtgagggc cgcgccggcg gccgatgagg agcgtagggc
//
LOCUS       DAOQYE010000888         1130 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_171186_length_1130_cov_2.670698, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000888 DAOQYE010000000
VERSION     DAOQYE010000888.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1130
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_171186_length_1130_cov_2.670698"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="PKN52_12015"
     CDS             <1..402
                     /locus_tag="PKN52_12015"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HNR00723.1"
                     /translation="TAYHFRLGTEADAVERPEQRADAGVLIEMMRRLTGHEPRMWGPS
                     IIGFGQYHYQYESGHEGDAPLAAFAPRKGNTVVYLTPALLADNPDLQRLGKHKASKGC
                     LYIKRLSDVDVAVLEGLVRACTELTRRQYGG"
     gene            399..989
                     /locus_tag="PKN52_12020"
     CDS             399..989
                     /locus_tag="PKN52_12020"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HNR00724.1"
                     /translation="MRPGQEPRRYTDQRFVGLTTAELPLENAIVIGCEFASCDLTEAS
                     LKGANLSECRFAGCELAMCDLTDAVFQSLEFSTCRLTGAHFETLSQLPIVPSAKFDEC
                     DLSYTSFARMDLREFAFTSCRFLEAEFTGCDLRAVDFGGSDLARCVFARNDMRGTDMR
                     TARNYVLSLQHNEVRGLRARLPEAAALLLAAGVELD"
BASE COUNT          191 a          393 c          372 g          174 t
ORIGIN      
        1 acggcttacc acttccggtt gggcacagag gccgacgccg tcgaacgccc ggagcagcgc
       61 gccgacgctg gcgtgctcat cgagatgatg cgccgactga ccggccatga gccaaggatg
      121 tgggggccga gcataatcgg gttcgggcag tatcactacc agtacgagag cggccatgag
      181 ggagacgccc cgctggccgc cttcgcgccc cgcaaaggca acacggtcgt ctacctgacg
      241 ccggcgctcc tggccgacaa ccccgacctg cagcggctcg gcaagcacaa ggccagcaaa
      301 gggtgcctgt acatcaagcg cctgtccgac gtcgacgtgg ccgtactgga gggactcgtg
      361 cgggcctgca ccgagctgac gcgccggcag tacgggggat gagacccggc caggagcccc
      421 gccgttacac ggaccagcgc ttcgtaggcc tcacgaccgc cgagctgcct ctggagaacg
      481 ccatagtgat cggctgcgag ttcgcctcgt gcgacctcac cgaggcctcg ctcaagggcg
      541 ccaacctctc cgagtgccgg ttcgcgggct gcgaactcgc catgtgcgac ctgaccgacg
      601 cggtcttcca gtccctcgag ttctccacct gtcggctgac cggcgcgcac ttcgagaccc
      661 tgagccagct gcccatcgtg ccgagcgcca agttcgacga gtgcgacctg agctacacgt
      721 ccttcgcgcg catggacctg cgggagttcg ccttcacttc gtgtcggttc ctggaggccg
      781 agttcacagg ctgcgacctg cgcgccgtgg acttcggcgg ctcggaccta gccaggtgcg
      841 tgttcgcgcg caacgacatg cgcggcaccg acatgcgcac ggcccgcaac tacgtcctct
      901 cgctccagca caacgaggtg cgcggtctgc gggcacggct gcccgaggcg gccgcattgc
      961 tgctggcggc tggggtggag ctcgattgac ggacactcac gatcgcggac cgcgcttgcc
     1021 acaagcaagc agcctgctgg agaacgccac gcggacgatg ccacgcttgg cgtagcctga
     1081 tggacctccg ctctggtacg gaccatggct ggagctgtgg cgccgaaacc
//
LOCUS       DAOQYE010000889         1130 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_171218_length_1130_cov_2.392558, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000889 DAOQYE010000000
VERSION     DAOQYE010000889.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1130
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_171218_length_1130_cov_2.392558"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            28..930
                     /locus_tag="PKN52_12025"
     CDS             28..930
                     /locus_tag="PKN52_12025"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNR00725.1"
                     /translation="MSAFPIDMDTLRQAAAAYKPGERRVWAFQITAQSGHTRDLTLSH
                     AEFLAMAEALTRLLERAAGREVLGVGVSMPGPFDYESGVSRMRGLGKFETIHDLALAR
                     EVAARETALAGLRWRWLNDARAFALGELRHGAAAGAKRAIFLTLGTGCGSAFAVDDRL
                     VTDGAGVPDGGYVYALRHGEHNIDELLSARGLLGLWHEVAKGDERGPSVRTAGDVGRL
                     AQSGDPRALEALRRFGVLIASALAPVFIEFEPDVIVLGGRVSRSLPYFGPAARAAGAP
                     PLVAAAEPDVAALRGAALHFLEGR"
BASE COUNT          139 a          402 c          402 g          187 t
ORIGIN      
        1 tataagtgag taacgtttca ggtcgatgtg tccgccttcc ccatcgacat ggacacgctg
       61 cgccaggcgg ctgccgccta caagcccggc gagcgccggg tatgggcctt ccagatcacc
      121 gcccagtccg ggcatacacg cgacctgaca ctgagccatg ccgagtttct ggccatggcc
      181 gaggccctca cgcggctgct ggagcgcgcg gctgggcgcg aggtgctcgg cgtcggcgtc
      241 tcgatgcccg gcccgttcga ctacgagtct ggcgtctctc gcatgcgagg gctcggcaag
      301 ttcgagacca tccatgacct agcgctggcg cgggaggtcg ccgctcgtga gactgccctg
      361 gcgggtctac gctggcgctg gctcaacgac gcgcgcgcct tcgcgctcgg cgaactgcgc
      421 cacggggcgg ccgccggcgc caagcgcgcc atcttcctca cgctcggcac gggttgcgga
      481 tcggcgttcg ccgtggacga ccggctggtc accgacgggg cgggggtgcc ggatggcggc
      541 tatgtgtacg ccctgcgcca cggtgagcac aacatcgacg agctgctctc ggctcgcggc
      601 ctgctcgggt tgtggcatga ggtcgcgaag ggtgacgagc gtggtccgag cgtgcggacc
      661 gccggcgacg tgggccggct tgcgcagagc ggcgacccgc gcgccctcga ggccctgcgc
      721 cgcttcgggg tcctcatcgc ctcggctctc gcgcccgtgt ttatcgagtt cgagcccgac
      781 gtcatcgtgc tcggcgggcg cgtcagccgc agcctgccgt actttgggcc cgccgcccgg
      841 gcggcggggg cgccgccgct cgtggccgcc gccgagccgg acgtcgccgc gttgcggggc
      901 gccgcgcttc acttcctgga ggggcgctga taagttgatg gttcgcgccg gctcgtgcag
      961 cgaccgcccc ggggcgaccg gacggtcgtc gatcgcttcc taggcttgtt caacaaccga
     1021 ccgcgcttct tgcctatgcc cgttgaggag gctcccgaca ccgaatgacc aagtactccg
     1081 accccatccg ccgcccggcc ggcgtttacg acgcggcgcc ggtccatgcg
//
LOCUS       DAOQYE010000890         1129 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_171501_length_1129_cov_2.015829, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000890 DAOQYE010000000
VERSION     DAOQYE010000890.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1129
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_171501_length_1129_cov_2.015829"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1129
                     /gene="glyS"
                     /locus_tag="PKN52_12030"
     CDS             <1..>1129
                     /gene="glyS"
                     /locus_tag="PKN52_12030"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF014183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HNR00726.1"
                     /translation="LLDLLGSRLAAVELVPESVTVYATPRRLAAWVRGLPDSTPERVE
                     ERRGPPANVAFTAEGELTKAATAFAAKNGVAASELVRSDDERGNYVYAKVHVGGRRTA
                     ELLPDLLAGVVRDLPAPRKMRWGDEPTPFIRPIAWLVALLDGEVVDVEVAGVRASNRS
                     RGHRFLAPEEFEVRGGEGYEEELARRFVIADRAKRRAATWEAVRAAAGSAGFTADDAP
                     ALLDEVTDLVESPFAILGAFGDEFLELPEEVLATVMIKHQRFFPTRAAGGAFAPAFVG
                     VANVRVPDEAVVRSGYEQVLAGRLYDARFFWRSDRRKSLSQHAWGLSGIAFQKELGSM
                     ADKTARVGVSARLLMDALGIGDDEAEAAKQALPIFSADLAT"
BASE COUNT          156 a          384 c          426 g          163 t
ORIGIN      
        1 gtctgcttga cctgctcggc tcccgccttg ctgcggtcga gctcgtgccg gagagcgtga
       61 cggtatacgc gaccccgcgc cgcctcgccg cctgggtgag gggtctgccc gactcgacgc
      121 ccgagcgggt cgaggagcgg cgcggcccgc ccgccaacgt ggcgttcacc gcggagggcg
      181 agttgacgaa ggccgccacg gccttcgccg ccaagaacgg cgtggccgcc tcggaactgg
      241 tgcgcagcga cgacgaacgc ggaaactacg tgtacgcgaa ggtgcacgtc ggcggtcggc
      301 ggaccgccga gctcctgccg gacctgctgg cgggcgtcgt gcgcgacctg ccagcgcctc
      361 gcaagatgcg atggggcgac gagccgacgc cgttcatcag gccgatcgcc tggctggtcg
      421 ccctcttgga tggcgaggtg gtcgacgtcg aggtcgcggg cgtgcgcgct tccaaccgct
      481 cacgaggtca ccgcttcctc gcccctgagg agttcgaggt ccggggcggg gaggggtacg
      541 aggaagaact tgccaggcgc ttcgtgatag cggaccgtgc caagcgtcgg gcggccacgt
      601 gggaggccgt gcgagccgcc gccggcagcg cagggttcac ggccgacgac gcgccggcgc
      661 ttctcgacga ggtgaccgac ctggtcgaga gcccgttcgc catcctgggc gccttcggcg
      721 acgagttcct cgagttgccg gaggaggtcc tcgcgaccgt gatgatcaag caccagcgct
      781 tcttcccgac cagggcggcg ggcggggcgt tcgcgccggc gttcgtcggc gtcgccaacg
      841 tccgggtgcc ggacgaggcc gtcgtgcgct ccggctacga gcaggtgctg gcgggtcggc
      901 tctacgacgc gcggttcttc tggcgctccg accggcgcaa gagcctgtcg cagcacgcgt
      961 ggggtctgag cggcatcgcc ttccagaagg agctcgggag catggccgac aagaccgccc
     1021 gcgtcggagt gtccgccagg ctcctcatgg acgcgctcgg catcggggat gacgaggccg
     1081 aggccgcgaa gcaggcgctg cccatcttca gcgcggacct agccaccga
//
LOCUS       DAOQYE010000891         1128 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_171608_length_1128_cov_4.147251, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000891 DAOQYE010000000
VERSION     DAOQYE010000891.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1128
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_171608_length_1128_cov_4.147251"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="PKN52_12035"
     CDS             <1..547
                     /locus_tag="PKN52_12035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00727.1"
                     /translation="CQVAVNFGTTGSRTVTVTTRDADGLSTTRSRTLNVQPPRANPYP
                     RITSYGVQARGLAAGNVCQNFGVASGTTIDIRENGCNLVGETGTHKRFSAFVTVENPD
                     NEALTYDWLVYVTYNDSEHILNNRTGLPDSTYVPVSPGNSGAETNPCRIAVHINAPEA
                     SRSKYLTVWSGSCTYDTTIVN"
     gene            747..>1128
                     /locus_tag="PKN52_12040"
     CDS             747..>1128
                     /locus_tag="PKN52_12040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00728.1"
                     /translation="MRRRTLASRLTLALIAMLATLLVSCGGTPGPSNLFVEENAWTGE
                     IPDGATIITSDEFRRMVGSGELVLRSSADVDAQAEELIRQFGENRDFLAGLTDAGENV
                     ADLLAAVAAGADYVGDVGVELDSGE"
BASE COUNT          211 a          387 c          361 g          169 t
ORIGIN      
        1 ctgccaggta gctgtgaact tcggaactac cggcagtagg accgtcaccg tgaccacgcg
       61 cgacgcggac ggcctcagca ccaccaggtc gaggaccctg aacgtgcaac cgccccgcgc
      121 caacccctac cccaggatca cgagctacgg cgtccaggcg cgcggcttgg ccgccggcaa
      181 cgtctgccag aacttcgggg tggcgtcggg tacgacgatc gatatccggg agaatggctg
      241 caacctcgtc ggcgagacgg gtacgcacaa gcgcttctcg gccttcgtta cggtcgagaa
      301 ccccgacaac gaggctctca cttacgactg gctcgtctac gtcacctaca acgactccga
      361 gcacatcctc aacaaccgca ccggcctgcc cgacagcacc tacgtgccgg tgtcgcccgg
      421 caactccggc gcggagacga acccctgcag gatcgccgtt cacatcaacg ccccggaagc
      481 gtcacgcagc aagtacctga cggtgtggtc gggcagttgc acctacgaca cgaccatcgt
      541 caactgaccg tcgagccggc ggacgcgcgc tttgcgcccc aagcacccct ataaccggta
      601 tataaccgcc acgcgactag gctcctgtca gtgacccagg ggtcggagcg ccatgcccgc
      661 gctcacgtcc ggacgggtcg cggtccggcg gttgcgccta cgacacgtcg acactcgact
      721 ggggaacggg ggaagaagga gcattcatgc ggcgtagaac cttggcctcg aggctcaccc
      781 tggcgctgat cgccatgctg gcgaccctgc tagtgtcctg cggcggtacg cccggaccga
      841 gcaacctgtt cgtggaggag aacgcctgga ctggcgagat ccctgacgga gcgaccatca
      901 tcacttcgga cgagttcagg cgcatggtcg gctcgggcga gctggtattg aggagctcgg
      961 cggacgtcga cgcccaggcc gaggagctca tcaggcagtt cggcgagaac cgtgacttcc
     1021 ttgctggtct caccgacgcc ggcgagaacg tggccgacct ccttgccgcc gtggcggccg
     1081 gcgccgacta cgtcggcgac gtgggggtgg agctcgacag cggcgagg
//
LOCUS       DAOQYE010000892         1128 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_171833_length_1128_cov_1.438024, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000892 DAOQYE010000000
VERSION     DAOQYE010000892.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1128
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_171833_length_1128_cov_1.438024"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            191..>1128
                     /locus_tag="PKN52_12045"
     CDS             191..>1128
                     /locus_tag="PKN52_12045"
                     /inference="COORDINATES: protein motif:HMM:NF024748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HNR00729.1"
                     /translation="MPDDPTSRMTDPSTKDPQDLRRRFEQRERVLGGLEPAEDEAQGA
                     APRVAGGEGAPGVLSVVAFPLDGGPGAGNAISYRADEPFPSASTIKVYVLQALLERVA
                     AGDADLDDELPVSANDQVSGSGILKALTPGRRYTLRDIATLMIVVSDNTATNVLIEYV
                     GLENLNASIRAHGWHGTRSAGKLQLAPVDGGPRRSPSTTTAADLADYFARLWRGELLP
                     APLTEEAKAIYRKQQFCEMGRSIGYDSYDASIGEAPWLIASKSGSIRGTRNDAGVFEP
                     VTAAAHGAKPFVIAIMTKGCVDERFHSDNLGARVVG"
BASE COUNT          193 a          400 c          389 g          146 t
ORIGIN      
        1 agagagtggg ccgtcagtga ctaccgggca cgagcaggcg tgcaccagcc ctacacccgg
       61 acccgccggt ggtccttgct ggcaccatgg tcggacgagt gaagtgtagg gcttacgggc
      121 gtgcctcgca agatggcccg cgaccaaagc gtgtaacact tgggccgata tcccggatac
      181 gaggtgcaac atgccagacg acccgaccag ccgcatgacc gacccttcca ccaaggaccc
      241 ccaggacctg cggcggcgct tcgagcagcg cgagcgcgtg ctcggcggcc tggagccggc
      301 cgaggacgag gcgcagggcg cggcgccgag ggtcgcaggg ggagagggcg cgcccggggt
      361 gctctccgtc gtggcgttcc cgctcgacgg cgggccgggc gcggggaacg ccatcagcta
      421 ccgggccgac gaaccgttcc catcggccag caccatcaag gtctacgttc tgcaggccct
      481 gttggaacgt gtggcggccg gagacgccga cctggacgac gagttgccgg tgagcgccaa
      541 cgaccaggtg tccggctcgg gcatactgaa ggctctcacg cccgggcgac gctacacgct
      601 cagggacatc gcgaccctga tgatcgtggt cagcgacaac acggcaacca acgtcctcat
      661 cgagtacgtc ggcttggaga acctcaacgc ctccatccgg gcccacggct ggcacggcac
      721 ccgcagcgcg ggcaagctgc agctggcgcc cgtcgacggc ggcccgcggc gctcgccgtc
      781 caccaccacg gccgccgacc tagcggacta cttcgcgcgg ctctggcgcg gcgagctcct
      841 ccccgccccc ctcacggagg aggcgaaggc catataccgc aagcagcagt tctgcgagat
      901 gggccggagc atcggttacg actcgtacga cgcctccatc ggcgaggcgc cgtggctcat
      961 cgcctccaag agcggctcca tccggggcac gcgcaacgac gccggcgtgt tcgagcccgt
     1021 gacggcggcc gcgcacggcg ccaaaccgtt cgtgatcgcc atcatgacca aggggtgcgt
     1081 cgacgagcgc ttccactcgg acaacctcgg cgcgcgcgtc gtggggtg
//
LOCUS       DAOQYE010000893         1127 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_171926_length_1127_cov_2.678172, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000893 DAOQYE010000000
VERSION     DAOQYE010000893.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_171926_length_1127_cov_2.678172"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="PKN52_12050"
     CDS             <1..370
                     /locus_tag="PKN52_12050"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HNR00730.1"
                     /translation="APLVPAGIDALAGLELGGVPLAVALSQVTGLPTLYVRKKAKEYG
                     TMRFAEGGDIAGRRLLVIEDVVTSGGQVVLSTRDLRGAGATVDAALCVIDREAGGGAA
                     LAAEGVALRALFTMSELKAV"
     gene            435..797
                     /locus_tag="PKN52_12055"
     CDS             435..797
                     /locus_tag="PKN52_12055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HNR00731.1"
                     /translation="MTQDTLFAKIVRREIPADIVYQDDLVTAFRDIHPQAPVHMLIVP
                     NAVIPTAADVGTEHEAALGRMFTVARRIAEQEGLGAGYRLIVNCKEHGGQEVYHLHMH
                     LLGGRALGPMLDHSFERG"
     gene            924..>1127
                     /locus_tag="PKN52_12060"
     CDS             924..>1127
                     /locus_tag="PKN52_12060"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNR00732.1"
                     /translation="MANEMTYRRLGSSGLKLSTVSLGGWTTYGQTVAEQERVTRLIER
                     AVELGVNYFDTADVYANGKCEEVM"
BASE COUNT          177 a          395 c          404 g          151 t
ORIGIN      
        1 ggcgccgctc gtgccggcgg gcatcgacgc cctggccggg ctcgaactgg gcggcgtgcc
       61 gctcgccgtg gcgctctcgc aggtgacggg cctccccacc ctgtacgtcc gcaagaaggc
      121 gaaggagtac ggcaccatgc gcttcgcgga agggggcgac atcgcgggca ggcgcctcct
      181 cgtgatcgag gacgtcgtca cgtcgggcgg ccaggtcgtg ctctcgaccc gcgacctgcg
      241 cggcgccggc gccaccgtcg acgcggcgct ctgcgtgatc gaccgggagg cgggcggcgg
      301 agcggccttg gcggccgagg gcgtggcgct ccgggcgctc ttcacgatga gcgagctgaa
      361 ggcggtctga gcgcggccga tacgccttct gcgtcgcaca gacgaccacg cgccgggtgc
      421 gcttacactt ggtcatgacc caggacacgc tcttcgccaa gatcgtgcgc cgggagatcc
      481 cggcggacat cgtctaccag gacgacctag tcacggcgtt ccgcgacatc cacccgcagg
      541 cacccgtcca catgctcatc gtgccgaacg ccgtcatccc gacggccgcc gacgtcggca
      601 ccgaacacga ggcggcgctc ggccgcatgt tcacggtggc gcgcaggatc gccgagcagg
      661 agggactagg cgcgggctac cgactgatcg tcaactgcaa agagcacggc ggccaggagg
      721 tctaccacct gcacatgcac ctcctgggcg gtagggcgct cggccccatg ctcgatcact
      781 ccttcgagcg gggctgaccg ggacaaccca cgacggccgc tcgggcgcgc cgggcggtca
      841 ggcgcgccgg gcgcgcccga caggcccggc ggtccggcag gcccggcgtc gtctctccga
      901 ccgtcgaggg gttagcctta gccatggcca acgagatgac ctaccgcaga cttggcagca
      961 gcggcctcaa gctcagcacg gtgagcttgg gcggctggac gacttacgga cagacggtgg
     1021 cggagcagga gcgcgtcact cgcctgatcg agcgcgccgt ggagctcggg gtcaactact
     1081 tcgacaccgc cgacgtctac gccaacggca agtgcgagga ggtcatg
//
LOCUS       DAOQYE010000894         1124 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_172860_length_1124_cov_1.438728, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000894 DAOQYE010000000
VERSION     DAOQYE010000894.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1124
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_172860_length_1124_cov_1.438728"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..973)
                     /locus_tag="PKN52_12065"
     CDS             complement(<1..973)
                     /locus_tag="PKN52_12065"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNR00733.1"
                     /translation="MESTRVVLVTNVGTGFGRAVALAYGRSGYHVVCADRDVDLAAKT
                     AAEVEELGGQAIPIQTDATTQMDVLSAYHKVMEIFGAIGGVVHLATQSSTTPFEDLAE
                     GEFSELMAENVRSSYLILKAASRLLGNGWVVLVAPPTEGSVQMRAVQGALKELVEGFR
                     HRFAYPRVNLVMPSRASSDPRHDQALVRAVRFLGSQESAGVSGQSVTVELPQPPRVTE
                     NLLPEVRAALDTSVRQSDEERWPGWEDGAWSEAASGASGAPGVEDAELESDDLEQDDL
                     EPDATALRAAPGVRRAGARLGFAGLEDDDEEVLAAARELEDDALDYFK"
BASE COUNT          176 a          410 c          368 g          170 t
ORIGIN      
        1 ccttgaagta gtcgagcgcg tcgtcttcca gttcccgagc ggcggcgagg acctcctcgt
       61 cgtcatcttc gagcccggca aacccgagcc tggcgcccgc ccgcctcacg cccggcgcgg
      121 cccgaagcgc ggtcgcgtcc ggttccagat cgtcctgctc caggtcgtcc gactccagtt
      181 cggcgtcctc cacgcccggg gctccggacg cgcccgacgc cgcttcggac catgcgccgt
      241 cctcccaccc cggccaccgc tcctcgtcgc tctggcggac cgacgtgtcg agcgcggccc
      301 ggacctcggg caagaggttc tcagtcacgc gtggcggctg cggaagttcg acggtgacgg
      361 actgcccgct caccccggcc gactcctgcg agccaaggaa acgcacggcc ctcacgaggg
      421 cctggtcgtg ccgcgggtcg gacgacgcgc gcgaaggcat gacgaggttg acgcgcggat
      481 aggcgaacct atgcctgaaa ccctccacca gctccttaag agcgccctgg acggcccgca
      541 tctgcaccga cccctccgtc ggcggtgcca cgagcactac ccagccgttg ccgagcagcc
      601 gcgaggcggc tttcaggatc aggtagctcg agcgcacgtt ctcggccatg agctcggaga
      661 actcgccctc ggccaagtcc tcgaacgggg tggtgctcga ctgcgtggcg aggtggacga
      721 cgcctccgat ggcgccgaag atctccatca ccttgtggta ggcggagagc acgtccatct
      781 gtgtggtcgc gtccgtctgg atggggatgg cctgaccgcc gagctcctcg acctcggctg
      841 ctgtcttggc ggccagatcg acgtcccggt cggcgcagac gacgtgatag ccggagcgac
      901 cgtaggccag tgcgacggca cgaccgaagc cggtcccgac gttcgtcact aacacgaccc
      961 gggtgctctc cattcccgta ggatacgacg caccaagggc accctcaact agcgcacgct
     1021 acccctgagg tcgcggacgc tgccccttga ggccggcgca aggaggtcta ggatgttcag
     1081 gcgacgcaag gaggaagcgg gggccgagcc tcgccccgag ccct
//
LOCUS       DAOQYE010000895         1123 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_172986_length_1123_cov_2.380150, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000895 DAOQYE010000000
VERSION     DAOQYE010000895.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1123
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_172986_length_1123_cov_2.380150"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            274..>1123
                     /locus_tag="PKN52_12070"
     CDS             274..>1123
                     /locus_tag="PKN52_12070"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I asparaginase"
                     /protein_id="HNR00734.1"
                     /translation="MTKKVLLAHTGGTIGMRRGRRGYAPSPGYLSELLARMPELNDPS
                     LPAIELVEFDPLLDSADMAPSDWSRIASEIARRHDAVDGVVVLHGTDTMAFTSSALSF
                     MLEGLAKPVIVTGSQVPLAEPRSDARENLIASLQIAALPGLHEVCVYFSGQLLRGNRA
                     TKVSASGFDAFASPNEAPLATVGVGIELRRGATRPAQPGPLRVHELRDVTVAAVRLFP
                     GVSARFMAHVLQEPLEGLVLETYGAGNAPTRDPRLLAELASAVERGVVVVNTTQCLRG
                     RVDMRGY"
BASE COUNT          158 a          423 c          369 g          173 t
ORIGIN      
        1 gcgacctgca cccgcgcgtg tggtccgaat gcctgcggtg tcccttctac gtcggttgca
       61 acgagaacgg cgtcgtcaag cgcatggacg gcgtgcccac tccccacaac ctgcccgaac
      121 cgccccccgc ccaggtgccg ccgggcatcc gctgaggcgc cggtagccgc tcgcccacgc
      181 ccacggcccg ccaacccaac gatgaacgat ccatatgccc gcgtgccctg acgcgtatgt
      241 ctcgcgtcag gaccgggtcg ttatagtcgc gtggtgacca agaaggtcct actagcccac
      301 accggcggaa cgataggcat gcgtaggggc aggcgtgggt acgcgcccag tcccggctac
      361 ctttccgagc tgctggcgcg catgcccgag ctgaacgacc cgtccctccc cgccatcgag
      421 ctggtcgagt tcgaccccct gctcgactcc gccgacatgg caccgtccga ctggtccagg
      481 atcgcgtccg agatagcgcg acgccacgac gccgtcgacg gcgtcgtcgt cctgcacggc
      541 acggacacca tggcgttcac ctcttcggcc ctctcgttca tgctcgaggg gctcgccaaa
      601 ccggtcatcg tcaccggttc ccaggtcccg ttggccgagc cgcgctcgga cgcgcgcgag
      661 aacctcatcg cctccctgca gatcgcggcg ctgcccgggc tgcacgaggt ctgcgtgtac
      721 ttctccggcc agctcctcag gggcaaccgc gccaccaagg tgtccgccag cggcttcgac
      781 gccttcgcgt cacccaacga ggcgccgctg gccaccgtcg gggtcgggat cgagctgcgc
      841 cggggggcga cgcgacctgc gcagccgggg cctctccggg tccacgagct gcgcgacgtc
      901 acggtggccg ccgtgcgcct cttccccggt gtcagcgcca ggttcatggc gcacgtgttg
      961 caggagccgc tcgaggggct cgtgctcgag acgtacggtg ccggcaacgc cccgacccgc
     1021 gacccgcgtc tgctcgcgga gctcgcgagc gcggtggagc ggggggtggt ggtggtcaac
     1081 acgacgcagt gcctccgtgg ccgcgtcgac atgcgtggct acg
//
LOCUS       DAOQYE010000896         1123 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_173000_length_1123_cov_2.268727, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000896 DAOQYE010000000
VERSION     DAOQYE010000896.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1123
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_173000_length_1123_cov_2.268727"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1123
                     /locus_tag="PKN52_12075"
     CDS             <1..>1123
                     /locus_tag="PKN52_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00735.1"
                     /translation="AGAAANGVGEAESGRLFDLLERFANYGFNKSHAAAYAILSYQTA
                     YLKAHYPVEFAAALLTVERGDSDKVAQYVADARHMGVEVSAPDVNTSRADFTPVGQLV
                     RFGLYGIKNVGEGAVDHILAERERGGPFKDLFDFCKRVDTTVVNRRAVEYLIKSGAMD
                     GLVPGRHAGGDSGARHLLLANLDLALKWGVSEREQAASGQFGLFGADEIAAPVLADAP
                     AIDELTLLRFEKEALGLYLSAHPMGSYPGLAEAASVPVDGVERRFRALQKDGVHGGRL
                     RVVLAGLLQNVVKRPTRKGGMMARFEIADESGSREVLAFGRTFDAVAPLLQEDAPVVA
                     VVELSEEGEDVRAVVDRLIRWDTREASQGGAPEVALFSFP"
BASE COUNT          165 a          380 c          407 g          171 t
ORIGIN      
        1 aggcgggcgc ggcagccaac ggcgtcggcg aggcggagtc cgggcgactc ttcgatctgc
       61 tcgaacgctt cgcgaactac ggcttcaaca agtcacacgc cgccgcctac gccatcctct
      121 cctaccagac ggcgtacctg aaagcgcact accccgtcga gttcgcggcc gccctcctca
      181 cggtcgagcg cggcgactcg gacaaggtcg cgcagtacgt cgccgacgcg cggcatatgg
      241 gagtcgaggt ctcggcgcca gacgtcaaca cgtcccgcgc cgacttcacg ccagtgggcc
      301 agctcgtgcg cttcggcctg tacggcatca agaacgtcgg cgagggcgcc gtcgaccaca
      361 tcctggccga gcgggagcgt ggcggtcctt tcaaggacct cttcgacttc tgcaagcgcg
      421 tcgacacgac ggtcgtcaac cgccgggccg tcgagtacct gatcaagtca ggggccatgg
      481 acgggctcgt gcccggcagg cacgcgggtg gcgactccgg cgcccggcat ctacttctgg
      541 ccaacctcga cctggccttg aagtggggcg tctcagagcg cgaacaggcc gcgtcgggcc
      601 agttcggcct cttcggcgcg gacgagatcg cggcgccggt gctggccgac gcgcccgcca
      661 tcgacgagct gacgctcctg cgcttcgaga aggaggcgct cggtctctac ctcagcgccc
      721 acccgatggg ttcgtacccc ggtctggccg aggccgcttc ggtgcccgtc gacggcgtcg
      781 agcggcgctt cagggcgctg cagaaggacg gggtgcatgg cggtcggctc cgggtggtgc
      841 tagccggcct gctccagaac gtggtgaagc gtccgacccg caagggcggc atgatggcgc
      901 gcttcgagat cgccgacgag tccggcagtc gcgaggtgct ggcgttcggc cgcacgttcg
      961 atgcggtggc gcccctgctg caggaagacg cgcccgtcgt ggcggtcgtg gagttgagcg
     1021 aggagggaga ggacgtgcgg gccgtcgtcg accgcctcat ccggtgggac acccgggagg
     1081 cgtcgcaggg aggggcgccg gaggtggcgc tgttcagctt ccc
//
LOCUS       DAOQYE010000897         1123 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_173022_length_1123_cov_2.120787, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000897 DAOQYE010000000
VERSION     DAOQYE010000897.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1123
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_173022_length_1123_cov_2.120787"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            111..>1123
                     /locus_tag="PKN52_12080"
     CDS             111..>1123
                     /locus_tag="PKN52_12080"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HNR00736.1"
                     /translation="MAGRPARVALIGGGTVGGAVVSLLEGRAEFELVGVLVRDPGRRR
                     AFARASELVTADPGVLEGADIVVEVAGGVGQAADLALRHLRRGLPLVTANKAALAERW
                     DEFLPHARDGRLHFEAAVMAGTPSVGVLAGCLRGSRPLALHAVLNGTCNVILAAMEEG
                     GTYASALAEAQRLGYVEADPTLDVEGLDAAHKLTVLGRLAFDPALDWQTVRAGTRGIS
                     RLTPAVIERELARGRHVRLVASVVPTAGSWAATVRPVSLPHAHPLLTTGATNALLFSG
                     DPIGHVVVRGPGAGGGATASGVVADLLAAANGAKGPLPLRAVAAAPAADEVAAVAAAA
                     GIE"
BASE COUNT          124 a          433 c          410 g          156 t
ORIGIN      
        1 gacgacttcc gaacccgaag cctatcagac gaaacgccag cacgcaggct cctgcgcgtc
       61 cgccgccccg tgcgcggtcg ccgcgcgtca tcggggcacg gggcttcgcc atggccggta
      121 gacccgcgcg ggtcgccctc atcggcggcg gcacggtcgg cggggcggtc gtctcgcttc
      181 tcgaaggtcg tgctgagttc gagctcgtcg gagtactggt gcgcgatccc ggccgccgcc
      241 gcgccttcgc gcgcgcctcg gagttggtta ccgcggaccc tggtgtcctc gaaggggccg
      301 acatcgtcgt cgaggtggcc ggcggcgtag gtcaggcagc cgacctggcg ctgcgccacc
      361 tccggcgcgg cctccccctc gtgaccgcca acaaggccgc cctggcggag cgctgggacg
      421 agttcctgcc ccacgcgcgc gacgggcgcc ttcacttcga ggccgccgtc atggccggta
      481 cgccctccgt cggcgtcctc gcgggctgcc tgcgtggctc gcgccccttg gcgctgcacg
      541 ccgtcctgaa cggcacgtgc aacgtcatcc tcgcggcgat ggaggagggc ggcacgtacg
      601 cgtctgccct ggcggaggcg cagcgcctcg gttacgtgga ggccgacccc acgctcgacg
      661 tggagggcct cgacgccgcc cacaagctca ccgtgcttgg cagactggcg ttcgaccccg
      721 ctctcgactg gcagacggtg cgtgccggca cgcgtggcat cagccggctc acgcccgccg
      781 tgatagagcg tgagctcgcc aggggccgcc acgtgcggct cgtcgcgagc gtcgtcccta
      841 ctgcggggag ctgggcggcc acggtgcgcc ccgtctcgtt gccacacgcc cacccgctcc
      901 tcacgaccgg cgccaccaac gcgctgctgt tctccggcga ccccatcggg cacgtcgtcg
      961 tgcgtggtcc gggcgccggc ggtggcgcga ccgcgagcgg ggtggtcgcc gacctcctcg
     1021 ccgcggccaa cggggccaag ggcccgctgc cgctgcgcgc ggtggccgcc gcgcccgcgg
     1081 ccgacgaggt ggccgccgtg gccgccgccg cggggatcga gga
//
LOCUS       DAOQYE010000898         1122 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_173248_length_1122_cov_2.539831, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000898 DAOQYE010000000
VERSION     DAOQYE010000898.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1122
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_173248_length_1122_cov_2.539831"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1122)
                     /gene="glgP"
                     /locus_tag="PKN52_12085"
     CDS             complement(<1..>1122)
                     /gene="glgP"
                     /locus_tag="PKN52_12085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HNR00737.1"
                     /translation="AQRDHLYAYLCAEYGWSEAIALYSGGLGVLAGDHTKAASDLGVP
                     LVAVGLYYPEGYFHQRVGPDGRQEAVYLRSSPAALPFRPAQNAAGERTVVSVEIFGRN
                     VLIQAWEVRVGRVRVLLLDVDMPGNEPADRKLLYRLYGGDQRTRVAQEMILGIGGVRM
                     LRALDVHPTAWHMNEGHSAFSVLERCRELVAAGLPFPAARAGVSAATVFTVHTPIAAG
                     NDAFGFDLVDQGFGRFYDGLRLSQAEFHDLGGVDLGWGPVFSMPALAIRFSTGRNGVA
                     ALHGDTSRHIWSRLWEGVPVAEVPIGHVTNGVHIATWTAPEVIDLIGDVIGPDWRERT
                     DDPDAWRAFDSVAAARLWQVRRQMKARSVRFLRRRLLSQL"
BASE COUNT          181 a          380 c          401 g          160 t
ORIGIN      
        1 cagttggctg aggaggcgcc tgcgtaggaa gcgcacgctg cgcgccttca tctgccgtct
       61 tacctgccat agccgggcgg cagcgacgga gtcgaacgcg cgccaggcgt cggggtcatc
      121 ggtacgctca cgccaatcgg gtcctatgac gtcgccgatc aggtcgatga cctcgggcgc
      181 cgtccaggtg gcgatgtgca cgccgttggt cacatgaccg atcggtacct cggccaccgg
      241 cacgccttcc caaagccgcg accagatgtg cctactcgtg tcgccatgca gcgcggcgac
      301 gccgttgcgt ccggtggaga agcggatggc gagtgccggc atgctgaaca cgggccccca
      361 accgaggtcg accccgccga gatcgtggaa ctccgcctgc gagaggcgca gtccgtcgta
      421 gaacctgccg aagccctggt cgacgaggtc gaagccgaag gcgtcgttgc ccgccgcgat
      481 cggggtatgg acggtgaaca ccgtcgcagc cgacacgccc gcacgcgccg ccgggaacgg
      541 cagacccgcc gcgacgagct cgcggcaccg ctcgagcacg gagaacgccg agtggccctc
      601 gttcatgtgc caggcggtgg gatgaacgtc cagggcgcgc agcatgcgta ccccgcctat
      661 cccgaggatc atctcctggg cgacgcgggt gcgctgatcg cccccgtaga ggcggtagag
      721 cagcttgcga tcggccggtt cgttgcctgg catgtcgacg tcgaggagca ggacccgaac
      781 acgcccgacg cgcacttccc acgcctggat gagcacgttg cgaccgaaga tctcgacgct
      841 cacgacggtc cgctcgccgg ccgcgttctg ggcgggccgg aacgggaggg ccgcggggct
      901 ggagcgtagg tagaccgcct cctggcgtcc gtccggtccc acgcgctgat ggaagtagcc
      961 ctcggggtag tagaggccca ccgctaccag ggggacgccg aggtcggacg cggccttcgt
     1021 atggtcgccg gccagcacgc cgaggccgcc ggagtagagc gcgatggcct cgctccagcc
     1081 gtactcggcg cagaggtagg cgtacaggtg gtcccgttgg gc
//
LOCUS       DAOQYE010000899         1120 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_173738_length_1120_cov_3.150235, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000899 DAOQYE010000000
VERSION     DAOQYE010000899.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1120
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_173738_length_1120_cov_3.150235"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(220..471)
                     /locus_tag="PKN52_12090"
     CDS             complement(220..471)
                     /locus_tag="PKN52_12090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00738.1"
                     /translation="MNKELIERFLKEMREKHSEQIDAVALPDGTAEFIAEVVASHDND
                     TVEFMVKLSYLMGLQTGFAAAVAGASGPGPEPGRGPLQA"
     gene            complement(468..959)
                     /gene="mgsA"
                     /locus_tag="PKN52_12095"
     CDS             complement(468..959)
                     /gene="mgsA"
                     /locus_tag="PKN52_12095"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HNR00739.1"
                     /translation="MKSVALIAHDKKKAELAEFVAGHAEVLGRFPLIATGTTGKVLMD
                     RTGLTIERLASGPLGGDLQVGARIAQDEVLAVIFFRDPLTAQPHEPDISALLRICDVH
                     RVALATNSASGEAVVAWLADLLADEELFGAHAAETGEAPGRTADRASSQPSRRTTDGA
                     TRP"
BASE COUNT          156 a          387 c          382 g          195 t
ORIGIN      
        1 ggtctggaag cctcggcggc gctcaggctc gccaacgtgt ggtgccagct gccgtcgcgg
       61 tcgagcgcca ccgtgccgct cgcgccagcc ggcggaccct cgaagaactc cgagaagacg
      121 aactcgaagt tgctgaggaa cacctcgatg ctcagtccca tgttcacgcc tctcttcctg
      181 ggtagccgtt cgaccccgtg cgggtcgcag gcggcttggt cacgcttgca gcggtccgcg
      241 ccccggctcc ggccctggtc cggaagcgcc cgccaccgct gcggcgaaac cggtctgcaa
      301 gcccatcagg tacgagagct tgaccatgaa ctcgaccgtg tcgttgtcgt gcgacgccac
      361 gacttcggcg atgaactcgg cggtgccgtc cggcaaagcc accgcgtcga tctgttccga
      421 gtgcttctcg cgcatctcct tgaggaagcg ctcgatgagc tccttgttca tggtcgcgtg
      481 gctccatccg tggttcggcg gctcggttgg ctcgaggctc gatccgccgt gcggcctggc
      541 gcctcaccgg tctccgccgc atgcgcgccg aacagctcct cgtcggccag gaggtccgcc
      601 aaccacgcca cgacggcctc gccgctcgcg gagttggtgg ctagcgccac gcggtggacg
      661 tcgcagatgc gcagcaaggc ggagatgtcg ggctcgtgcg gctgggccgt caggggatcg
      721 cggaagaaga tgacggcgag cacctcgtcc tgtgcgatgc gggccccgac ctggaggtcg
      781 ccgccgagcg gtccggacgc caggcgttcg atggtgaggc ccgtcctgtc catcaggacc
      841 ttgcccgtcg tgcccgtggc gatcagcggg aagcgcccga gtacctcggc gtgcccggcc
      901 acgaactccg cgagctcggc cttcttcttg tcgtgcgcga tgagtgccac ggacttcatg
      961 gcctcaagtg tagggcctcc gggggtgagg atgcgtctca gccgcggctc cgcgccgcac
     1021 gcgtccatac ggcgcaggtt tagcatggag ctgtggaact tgccatggcg gtcgcggcgg
     1081 ccctcacgat cgtggcgatc ctgagcaccc ggctctcgca
//
LOCUS       DAOQYE010000900         1120 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_173856_length_1120_cov_2.115493, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000900 DAOQYE010000000
VERSION     DAOQYE010000900.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1120
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_173856_length_1120_cov_2.115493"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..778)
                     /locus_tag="PKN52_12100"
     CDS             complement(<1..778)
                     /locus_tag="PKN52_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNR00740.1"
                     /translation="MTGSPSRAPGRRSAGKVTVKVIGKALFWLLIAVMFVYLMFPFYW
                     ALVSALKSQPELIRTPATLWPAEPVLTNFKAVLSNDRFVRAMLVSTLVASCVTLLSLA
                     AGSFAGYALGKMRFRGRSPSLYLILAMTMFPQIAVLAGLYAVIRLLALPAVPSMILSY
                     MLFTLPFTVWVLTSFFRGLPDSLLQAAQVDGATLFQAFRHVLLPLTAPALVTTGLLAF
                     IQAWNEYLFALTFTVIDPPARTVPVAIALFTGEIARQETFG"
     gene            complement(775..>1120)
                     /locus_tag="PKN52_12105"
     CDS             complement(775..>1120)
                     /locus_tag="PKN52_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNR00741.1"
                     /translation="LLLLAGLSTIPSEYAEAAKVDGAGVIRRFFSITLPLLTPTLAVA
                     LIFRTLDSLRVFDIFQVVLGNQRYSMASFAQDMLISRRDMGLSSAASVTIFALIFVFA
                     ILYVRSMKVDTQ"
BASE COUNT          228 a          334 c          410 g          148 t
ORIGIN      
        1 cgccgaacgt ctcctggcgg gctatctcgc ccgtgaacag cgcgatggcg acgggcaccg
       61 tccgcgccgg cggatcgatc accgtgaacg tgagcgcgaa caggtactcg ttccaggctt
      121 ggatgaacgc gagtaggccg gtggtgacga gcgcgggcgc cgtcagcggc agcaggacgt
      181 gtctgaacgc ctggaacagc gtcgccccgt cgacctgggc cgcttgcagg agcgagtcgg
      241 gcaaccccct gaagaacgag gtgagcaccc agaccgtgaa cggcagcgtg aagagcatgt
      301 aggagaggat catgctcggg acggccggca gcgccaggag gcggatgacc gcgtagagcc
      361 cggccagcac ggcgatctgc gggaacatcg tcatggcgag gatgagatag agcgacgggc
      421 tgcgccccct gaagcgcatc ttgccgagcg cgtagccggc gaaggagccg gcggcgagcg
      481 acagcagcgt cacgcagcta gccacgagcg tcgatacgag catggcgcgc acgaagcgat
      541 cgttgctcag cacggccttg aagttcgtca gcaccggctc cgccggccac agggtggcag
      601 gggtcctgat cagctccggc tgcgacttga gggcagaaac gagcgcccag tagaacggga
      661 acatcaggta gacgaacatg acggcgatga ggagccagaa gagggccttg ccgatcacct
      721 tgacggtgac cttgccggct gaccggcggc ctggcgcgcg gctgggagac cccgtcattg
      781 cgtgtcgacc ttcatggagc gtacgtagag gatggcgaag acgaagatga gggcgaagat
      841 ggtcaccgac gccgccgacg acagccccat gtcgcgccgg ctgatgagca tgtcctgcgc
      901 gaagctcgcc atcgagtagc gctggttgcc cagcactact tggaagatat cgaacacgcg
      961 gagcgagtcg agggtgcgga agatcagcgc gacggccagg gtgggcgtga gcaggggcag
     1021 ggtgatgctg aagaagcgcc tgatgacccc ggccccatcg accttggcgg cctccgcata
     1081 ctcgctcggg atggtggaga gccccgccag cagcagcagc
//
LOCUS       DAOQYE010000901         1119 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_174156_length_1119_cov_1.925752, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000901 DAOQYE010000000
VERSION     DAOQYE010000901.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1119
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_174156_length_1119_cov_1.925752"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1119
                     /locus_tag="PKN52_12110"
     CDS             <1..>1119
                     /locus_tag="PKN52_12110"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNR00742.1"
                     /translation="DVTYDGNGNIVTAVRTGVEVVVQSSVSSDLSRAVLIAVRNWLTD
                     VYGESVVFGRGGLRVVTTIDVQAQVAANEASLHAELPPGAQMAIVGLDPSTGAVLAMV
                     GQKLSADGALGEFNRATRALRQPGSSFKPIVFATAIELGAMNQSTILVDEPTEFRMRG
                     QPPYEPGNHDKKFDGYQTIRASLNRSRNIPAVKALEAVGADAVAEKARELGYDVAPYY
                     SMALGTFEATPLQHTAAMAAFANGGVYTEPYFIERVEDADGNVLYQAAPRSARVWSEQ
                     TAYIMLDLLHGNVVDRDPAYGLSNRAVIPGRWIAGKTGTTNEEVDIWFVGMTPGMVAS
                     VWIGNDDSSSLPSSMTLADGTRDQVNSSRQPIYAWNDF"
BASE COUNT          198 a          383 c          382 g          156 t
ORIGIN      
        1 gggacgtcac gtacgacggc aacggcaaca tcgtcacggc ggtcaggacc ggcgtcgagg
       61 tggtcgtgca gtcgagcgtc agctcagacc tgtcgagggc cgtcttgatc gccgttcgca
      121 actggctcac cgacgtgtac ggcgagagcg tcgtgttcgg caggggcggc ctcagggtcg
      181 tgaccaccat cgacgtgcag gcgcaggtgg ccgccaacga ggcgagcctc cacgccgaac
      241 tcccaccggg tgcccagatg gccatcgtcg gcctcgatcc gagcacgggc gccgtgctgg
      301 cgatggtagg gcagaagctg agcgcggacg gcgcgctggg cgagttcaac cgcgccaccc
      361 gggcgctccg tcaaccgggc agctcgttca agccgatcgt gttcgccacg gccatcgagt
      421 tgggagccat gaaccagtcg accatcctgg tcgacgagcc caccgagttc cgcatgcggg
      481 gccagccgcc ctacgaaccc ggcaaccacg acaagaagtt cgacggctac cagacgataa
      541 gggcgagcct gaaccgcagc cgcaacatcc cggcggtcaa ggcgctcgag gccgtgggcg
      601 ccgacgcggt ggcggagaag gcgcgcgagc tgggctacga cgtcgcgccc tactactcga
      661 tggcgctcgg cacgttcgag gccacgccgc tgcagcacac agccgccatg gcggccttcg
      721 cgaacggcgg cgtctacacc gagccgtact tcatcgagcg ggtggaggac gcggacggca
      781 acgtgctcta ccaggccgcc ccacgctcgg cgcgcgtctg gagcgagcag acggcctaca
      841 tcatgctcga cctcctgcac ggcaacgtcg tggatcgcga cccggcgtac ggcctctcga
      901 accgcgccgt gatcccgggc cgctggatcg cggggaagac cggcaccacc aacgaggagg
      961 tcgacatctg gttcgtgggc atgaccccgg gtatggtggc gtccgtgtgg atcggcaacg
     1021 atgactcctc cagcctgccg tcgtcgatga ccctggcgga cggcacccgc gatcaggtga
     1081 acagttcgcg tcaacccatc tacgcctgga acgacttcg
//
LOCUS       DAOQYE010000902         1117 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_174630_length_1117_cov_2.245763, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000902 DAOQYE010000000
VERSION     DAOQYE010000902.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_174630_length_1117_cov_2.245763"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1117
                     /gene="carB"
                     /locus_tag="PKN52_12115"
     CDS             <1..>1117
                     /gene="carB"
                     /locus_tag="PKN52_12115"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177002.1"
                     /note="four CarB-CarA dimers form the carbamoyl phosphate
                     synthetase holoenzyme that catalyzes the production of
                     carbamoyl phosphate; CarB is responsible for the
                     amidotransferase activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HNR00743.1"
                     /translation="ITVAPAQTLSDKEYQRLRDYSIAIIREIGVETGGSNIQFAVNPV
                     DGDVIVIEMNPRVSRSSALASKATGYPIAKIAALLAVGYYLDELPNDITKETKAAFEP
                     TIDYVVTKVPRFAFEKFPTSPTELGTQMRSVGEVMAIGRTFKESLLKAMRSQELDVRG
                     ATATAADGDLEALLHPNPRRLPAVLELLRRGRSVQSLHAATGIDPWFLTQLKEIVQAE
                     REVREGPPIEAWTWERWREVKRIGLSDREVAELTGADPGAVRAARIANDGAPVYKTVD
                     TCAAEFEAYTPYLYSTYEWEDEAPPSPRRKVVILGSGPNRIGQGVEFDYATVHAVWAL
                     KDAGFETVMVNSNPETVSTDYDTADRLYFEPLTFEDVS"
BASE COUNT          194 a          409 c          355 g          159 t
ORIGIN      
        1 catcacggtg gcgcccgccc agacactgtc agacaaggag taccagcgcc tgcgcgacta
       61 ctccatcgcc atcatccgcg agatcggcgt cgagacgggc ggctccaaca tccagttcgc
      121 cgtcaacccc gtcgacggcg acgtcatcgt catcgagatg aacccgcgcg tgtcgcgctc
      181 ctcggccctg gccagcaagg cgaccggcta ccccatcgcc aagatcgcgg ccctgctcgc
      241 cgtgggctac tacctcgacg agctcccgaa cgacatcacg aaggagacga aggcggcctt
      301 cgaaccgacc atcgactacg tcgtcacgaa ggtcccgcgc ttcgccttcg agaagttccc
      361 gacgtcgccg acggagctcg gcacgcagat gcgctcggtc ggcgaggtga tggccatcgg
      421 gcgcaccttc aaggaatcgc tcctgaaggc catgcgctcg caggagctgg acgtgcgggg
      481 ggctacggcc accgccgccg acggcgacct cgaggcgctg ctccacccca acccgcgccg
      541 cctcccggcg gtgctggagc tgttgcgccg cggccgctcg gtgcagtcgc tccacgcggc
      601 caccggcatc gacccgtggt tcctcaccca gctcaaggag atcgtccagg cggagcgcga
      661 ggtgcgcgaa ggcccgccca tcgaggcctg gacctgggag cggtggcgcg aagtcaagcg
      721 cattggccta tcggatcgcg aggtggccga actaacgggc gccgaccctg gcgccgtgag
      781 ggccgcgcgc atcgccaacg acggcgctcc cgtctacaag acggtcgaca cgtgcgcggc
      841 ggagttcgag gcgtacaccc cttacctcta ctccacctac gaatgggagg acgaggcgcc
      901 gccctccccc cgccggaagg tggtcatcct cggctccggt cccaaccgta tcgggcaggg
      961 tgtcgagttc gactacgcca ccgtccacgc cgtgtgggcg ctcaaggacg ccggcttcga
     1021 gacggtgatg gtcaactcga accccgagac cgtctcgacg gactacgaca cggccgatcg
     1081 cctctacttc gagccgctaa cgttcgagga cgtcagc
//
LOCUS       DAOQYE010000903         1116 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_174857_length_1116_cov_2.382658, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000903 DAOQYE010000000
VERSION     DAOQYE010000903.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_174857_length_1116_cov_2.382658"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /gene="bshC"
                     /locus_tag="PKN52_12120"
     CDS             <1..464
                     /gene="bshC"
                     /locus_tag="PKN52_12120"
                     /inference="COORDINATES: protein motif:HMM:NF021586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bacillithiol biosynthesis BshC"
                     /protein_id="HNR00744.1"
                     /translation="VLEPVAVRLLDGLGVSAARFRAQRTELLERTLLERHGHAELFAA
                     AAGCIESEFEKLLSEVGGIDPTLGGTVKRGRHHLEETLARLRGKTSAALAARDEVTTR
                     QFDRLAAHLLPLGQPAERVLSPFSHALKFGIEPLMDRFATLAPERDQELVL"
     gene            complement(563..>1116)
                     /locus_tag="PKN52_12125"
     CDS             complement(563..>1116)
                     /locus_tag="PKN52_12125"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HNR00745.1"
                     /translation="ERKNAVEKLDSILEVADVLRGSDVTLVADGGIKYSGDVAKAIAA
                     GADVVMVGSLLAGTSEAPGEEILRDGRRYKAYRGMGSLGAMAGGSADRYFQDASSGNQ
                     AKLVPEGIEGMVAYKGPVGDVIYQAAGGLRSAMGYCGTPDIAALQRDARFVTITQASL
                     VESHPHDVTITKDAPNYSVSKKG"
BASE COUNT          156 a          399 c          387 g          174 t
ORIGIN      
        1 cggtgctcga gcccgtggcc gtccggctcc tcgacgggct tggggtcagc gcggcacgtt
       61 tcagggcgca acggaccgaa ctgctggaac gcacgctcct ggagcgccac gggcacgctg
      121 aactgttcgc ggcggccgcc ggctgtatcg agtcggagtt cgagaagctc ctctccgagg
      181 tagggggcat cgacccgacg ctgggcggca cggtgaagcg tggcaggcat cacctggagg
      241 agaccctggc gcgcctgcgc ggcaagacga gcgcggcgct cgctgcgcgc gacgaagtaa
      301 cgacccggca gttcgaccgc ttggccgcgc acctcctgcc cctcgggcag ccggccgaac
      361 gcgtgctgag cccgttcagc cacgcgctca agttcggtat cgagccgctg atggaccggt
      421 tcgccaccct ggcccctgag cgcgaccagg aactcgtcct ctgatcggtt agcctcggcc
      481 gcggaccgtt ccacgagctg gggaccgttc cgcgagcgac cccgggcgac cgacgtcgac
      541 cgtaccgcgt gccgccctgc gctcagccct tcttagagac cgagtagttg ggagcgtcct
      601 tcgtgatggt cacgtcgtgc gggtgcgact ctacgaggct cgcctgcgtg atcgtcacga
      661 acctggcgtc gcgctgcagc gccgctatgt cgggcgtgcc gcagtagccc atggccgagc
      721 gcaggccgcc cgcggcctgg tagatgacgt caccgaccgg acccttgtag gcgaccatgc
      781 cctcgatgcc ctcggggacg agcttggcct gattgccgga ggaggcgtcc tggaagtagc
      841 ggtcggcgct cccgcccgcc atggccccca ggctgcccat gccccggtac gccttgtagc
      901 gccggccgtc ccgaaggatc tcctcgccgg gcgcctcgct cgtgccggcc agcagagagc
      961 cgaccatcac gacgtcggcg ccggccgcga tggccttggc gacgtcgccc gagtacttga
     1021 tgccgccgtc ggccacgagc gtgacgtccg agcccctgag gacgtcggcg acctcgagga
     1081 tggagtcgag cttctccacg gcgttcttgc gctcga
//
LOCUS       DAOQYE010000904         1115 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_175150_length_1115_cov_2.240566, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000904 DAOQYE010000000
VERSION     DAOQYE010000904.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_175150_length_1115_cov_2.240566"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1115
                     /locus_tag="PKN52_12130"
     CDS             <1..>1115
                     /locus_tag="PKN52_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HNR00746.1"
                     /translation="FDLWQTYGFPLDLTSEMAAERGVEVDAEGYEAAREHARELSRGG
                     TVGRELFAATSDALGKVAGRIGETEFLGYHETESEAVVAALVTPGDAAEETDALREGA
                     TGTVILDRTPFYPEGGGQVGDAGVLEWEGGKAVVSGTSKSRHGLTLHHARVVRGTLTR
                     GQRVVARVDPARRETEKHHSATHLLHAALRSVLGSHVAQAGSLVAADRLRFDFSHPHA
                     VSPSEQRRVEELANQWIQADVPVEWRVVPIAEARAAGAMMLFGEKYGENVRMVSIGAP
                     SHAVSRELCGGTHVTRTGQIGPFVIVAEEAVSSGVRRIEARVGASALRYLQELRDTQG
                     RLAAHLGGRPAELEERLAKLQADHKEALKEAARLRDRL"
BASE COUNT          163 a          387 c          430 g          135 t
ORIGIN      
        1 ttcgacctct ggcagacgta cggcttcccc ctcgacctga cgagcgagat ggcggccgag
       61 cggggcgtcg aggtggacgc cgaaggttac gaggcggcgc gcgagcacgc acgcgagctg
      121 tcgcgcggcg gcaccgtggg gcgcgagctc ttcgccgcga cgtccgacgc gctcggcaag
      181 gtggccgggc ggatcggcga gacggagttc cttggttacc acgagacgga gagcgaggcc
      241 gtggtcgccg cgctcgtcac gccgggcgac gccgcagagg agaccgacgc gctgcgcgaa
      301 ggcgccaccg gcacggtgat cctggaccgc acgcccttct accccgaggg cggcgggcag
      361 gtcggcgacg ccggggtgct cgagtgggag ggtggcaagg ccgtcgtgag cggcacgagc
      421 aagtcgcgcc acggccttac gctgcaccac gcccgcgtcg tgcgcgggac cctgacgcgc
      481 ggtcagcgcg tcgtggcgcg cgtggacccg gcgcggcgcg agacggagaa acaccactcc
      541 gccacgcacc tcctgcatgc cgcgctgcgc agcgtgctcg gctcgcacgt cgcgcaggcc
      601 ggctcgctcg tcgcggccga caggctgcgc ttcgacttct cgcacccgca tgccgtgagc
      661 ccgagcgagc agcgccgggt cgaggagctg gcgaaccagt ggatccaggc cgacgtgccc
      721 gtagagtggc gcgtggtgcc catcgcggag gcccgtgccg cgggggccat gatgctcttc
      781 ggcgagaagt acggcgagaa cgtgcgcatg gtgagcattg gcgcgccgtc tcacgctgtg
      841 tcgcgcgagc tgtgcggcgg cacgcacgtc acgaggacgg gccagatcgg cccgttcgtc
      901 atcgtcgccg aggaggcggt gtcgtccggc gtgcggcgca tcgaagcgcg agtaggcgcg
      961 tcggcgttgc gctacctcca ggaactccgc gacacccagg gacgcctcgc ggcgcacctg
     1021 ggcgggcgcc cggccgagct ggaagagcgc ctcgccaagc tccaagcgga ccacaaggaa
     1081 gcgctcaagg aggccgcgcg cctgcgcgac cgcct
//
LOCUS       DAOQYE010000905         1113 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_175603_length_1113_cov_2.511342, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000905 DAOQYE010000000
VERSION     DAOQYE010000905.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1113
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_175603_length_1113_cov_2.511342"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /locus_tag="PKN52_12135"
     CDS             complement(<1..637)
                     /locus_tag="PKN52_12135"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HNR00747.1"
                     /translation="MVLVITTSLDGRLAKSALELVSVARSLSQDVGGVVVGQRAAADA
                     LAAYVPTVHHVAADQLVAEAVTTTVCRLAGESGAATLLFSANRLGQSVAPRVAVRLGA
                     ALLEDVIALETAGAGLQAKRYSYLARVTETVRTSGAAGTTVVSVKPNVFKAAEPAGQG
                     VVTPYASEPLPTDARVGVGERSASVGGRVALEEAKVVVAGGPGTDLEELVAA"
     gene            complement(649..>1113)
                     /locus_tag="PKN52_12140"
     CDS             complement(649..>1113)
                     /locus_tag="PKN52_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta"
                     /protein_id="HNR00748.1"
                     /translation="VLAKVVQAEGAELVFVGGKQADWDSAALGPALAEVLGWPHSDWT
                     TALSVSGGVAHVKHDVDGGQEALTMPLPAVVTTQQGLNEPRYPTLPNIMKAKRKELVK
                     RELAEFAPGAALTELVSQDIQTQGRRGHIVEGDAVSAARELVRLLRDEAKVI"
BASE COUNT          150 a          379 c          415 g          169 t
ORIGIN      
        1 cggcggcaac cagttcttcg agatcggtgc ctggaccgcc ggccacgacg accttggcct
       61 cctcgagggc gacgcgccct ccgacggagg cgctgcgctc gcccacgccc acgcgcgcgt
      121 ccgtgggaag cggctcggag gcgtaaggcg tcacgacacc ctgcccggcc ggttcggcgg
      181 ccttgaagac gttcggcttg acgctcacga cggtggtgcc ggccgccccg ctcgtgcgga
      241 cggtctcggt gacgcgggcg aggtagctgt agcgcttagc ttgcagcccg gcgccagcgg
      301 tctccagggc gatgacgtcc tcgaggagcg cggcgccgag gcgcacggcc acccgaggcg
      361 ccacgctctg gccgaggcgg ttggcgctga agagcagcgt ggcggccccg gactcgccgg
      421 ccaaccggca cacggtggtc gtgacggctt cggccaccag ttggtcggcg gccacgtggt
      481 ggaccgtcgg cacgtaggcg gccaacgcgt cggcggcggc gcgctgaccg acgacgacgc
      541 cgccgacgtc ctgtgagagg cttctagcca cggagacgag ttcgagggcg ctcttggcga
      601 ggcgaccgtc gagggaggtg gtgatgacga gtaccatggc agccttcctt agatgacctt
      661 ggcctcgtcg cgcaggaggc ggacgagttc cctggcggcg ctgacggcgt cgccttcgac
      721 gatgtgaccg cggcgaccct gcgtctggat gtcctgcgac accagttcgg tgagggcggc
      781 gcccggcgcg aactcggcga gctcgcgctt gaccagttcc ttgcgcttgg ccttcatgat
      841 gttggggagc gtagggtagc ggggctcgtt cagcccttgc tgggtcgtga caaccgcggg
      901 aaggggcatg gtcaaagcct cctgtccgcc gtcgacgtcg tgcttgacgt gcgcgacgcc
      961 gccgctgacg ctgagcgccg tcgtccagtc ggagtggggc caccccagga cctcggctag
     1021 agccggcccg agggcggcgc tgtcccagtc cgcctgcttg ccgccgacga agacgagctc
     1081 ggcgccctcc gcctgaacca ccttggcgag gac
//
LOCUS       DAOQYE010000906         1109 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_176667_length_1109_cov_2.998102, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000906 DAOQYE010000000
VERSION     DAOQYE010000906.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_176667_length_1109_cov_2.998102"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1072)
                     /locus_tag="PKN52_12145"
     CDS             complement(<1..1072)
                     /locus_tag="PKN52_12145"
                     /inference="COORDINATES: protein motif:HMM:NF022114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gmad2 immunoglobulin-like domain-containing
                     protein"
                     /protein_id="HNR00749.1"
                     /translation="MATALPNSLTLAGGVVTLAYPAALALATSGQPLQVAATIPPCDA
                     GFDYCFHLSEGSFAGTNFRAAGMSVTLRKDLTAQLSCLLAQPSGFQDLQPGLAVPALE
                     GPTQDKSGSTGTDSATEARVAATIGPSTSRFGGLGEGAAGTYYNGEERRLFIDGACYE
                     FTTRVVESQFGNYPEGAVVEFTAEQRAAVLSQFDEVLASATVTVGGEIEPVEWPREGG
                     SDLAPFIRLEEPAAGEAVTSPLRLVGEAVGPWYFEGSFPVSVVAGDGSVLGQGFVSAQ
                     GEWMVDGLVPFVGEVEFEAPQAPDGSPDGSPDGQADGQADGSAAPDATATGLADPGTP
                     VTVVLARDNPSGLPEHDAALHVK"
BASE COUNT          166 a          407 c          392 g          144 t
ORIGIN      
        1 ccttcacatg caaggccgcg tcatgctccg gcagaccaga agggttgtcg cgcgccaaca
       61 cgaccgtcac gggtgtgccg ggatcggcga ggcccgtcgc ggtagcgtca ggcgccgccg
      121 aaccgtccgc ctgaccgtcc gcctgaccgt ccggcgaacc gtcaggcgaa ccgtcaggcg
      181 cctgcggcgc ctcgaactcg acctcgccca cgaacgggac caggccgtcc accatccact
      241 ccccttgggc gctgacgaac ccctggccga ggacgctgcc gtcccccgcg acgacgctca
      301 ccgggaacga gccctcgaag taccaaggcc cgaccgcctc gccgaccaag cggagcgggc
      361 tcgtgaccgc ctcgcctgcg gccggctcct ccaaccgtat gaagggggcg aggtctgacc
      421 cgccctcgcg cggccactcg acgggttcga tctcgccgcc gaccgtcacc gtcgcgctcg
      481 cgagcacctc gtcgaactgc gagaggaccg ccgcgcgctg ctcggccgtg aactcgacca
      541 ccgcgccttc ggggtagttg ccgaactgcg actccacgac gcgggtcgtg aactcgtagc
      601 aggcgccgtc aatgaagagg cgacgctcct cgccgttgta gtacgtgccg gcggcgccct
      661 cgcccaggcc gccgaagcgg gacgtggacg ggccgatagt cgcggcaacg cgggcctcag
      721 tggccgagtc cgtgcccgtt gagccgctct tgtcctgggt ggggccctcg agcgccggca
      781 ccgccagccc aggctgcagg tcctggaaac cgcttggttg ggcgagcagg cagctcaact
      841 gggcggtgag gtccttgcgg agcgtcacgc tcatgccggc ggcgcggaag ttggtgccgg
      901 cgaaggagcc ttcggacagg tggaagcagt agtcgaagcc ggcgtcgcac ggagggatgg
      961 tggcggcgac ctggaggggc tggccgctcg tcgcgagcgc cagcgcagcg gggtaggcga
     1021 gggtcacgac cccgccagcg agcgtcaggg agttcggcag cgcagtggcc accgcggccg
     1081 ccccggtccc ccgatcctct ccgaccggc
//
LOCUS       DAOQYE010000907         1109 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_176780_length_1109_cov_2.139469, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000907 DAOQYE010000000
VERSION     DAOQYE010000907.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_176780_length_1109_cov_2.139469"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1109)
                     /locus_tag="PKN52_12150"
     CDS             complement(<1..>1109)
                     /locus_tag="PKN52_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HNR00750.1"
                     /translation="LALPSPTQFLRIAKGDAKRWLRLLTQVPADFKLNSKIARQLKTR
                     EAMADGEVPLDWAAGEALALASLVAGGVRVRFSGQDVERGTFSHRHAVFHDAGDGHEY
                     VPMSAVEAHPGLFDIHNSPLSETGVLGFEYGYSLDAPDGLVLWEAQFGDFANTAQVII
                     DQFIASAEEKWSRLSGLVMLLPHGYEGQGPEHSSARLERYLQLCAEDNMQVVYPTTPA
                     QIFHLLRRQVVRPYRKPLVVMAPKSLLRHPRATSSLEELARGKFRRVIGDHDVDAKGV
                     SRILLTSGKVYYDLLAEREKRGATDVAIVRLEQLYPLHLAPLAEALEAFPADAPVYYV
                     QEEAENNGAWHHLLVRFGRELLGRPFSGVHRAAAA"
BASE COUNT          156 a          391 c          393 g          169 t
ORIGIN      
        1 tcgccgcggc cgcgcggtgg acgccgctga acggccgccc gagcagctcg cgcccgaagc
       61 ggacgagcag gtgatgccag gcgccgttgt tctcggcctc ctcctgcacg tagtagaccg
      121 gcgcgtccgc cgggaacgcc tcgagcgcct ccgccagcgg tgccaggtgg agcgggtaca
      181 actgctcgag gcgcacgatg gccacgtcgg tcgctccgcg cttctcgcgc tccgccagca
      241 ggtcgtagta gaccttgccg ctggtgagca ggatcctgct cacgcccttc gcgtccacgt
      301 cgtggtcgcc gatgacgcgc ctgaacttgc cgcgcgccag ctcctcgagc gagctcgtcg
      361 ctcgcgggtg gcgcagcagg ctcttgggcg ccatcacgac caacggcttg cggtaaggcc
      421 tcacgacctg gcgccgcagc aggtggaaga tctgggccgg ggtcgtgggg tagacgacct
      481 gcatgttgtc ctcggcgcag agctgcaggt aacgctcgag gcgcgcgctg gagtgctcag
      541 gcccctggcc ctcgtagccg tgcggcagca gcatgaccag gcccgacagg cgcgaccact
      601 tctcctcggc gctggcgatg aactggtcga tgatcacctg ggcggtgttc gcgaagtcgc
      661 cgaactgcgc ctcccacagc accagcccgt cgggggcgtc gaggctgtag ccgtactcga
      721 agccgagcac gcccgtctcc gacaggggcg agttgtggat gtcgaagagc ccggggtggg
      781 cctccaccgc gctcatgggc acgtactcgt ggccgtcgcc ggcgtcgtgg aacacggcgt
      841 gccggtggct gaaggtgccg cgctccacgt cctggccgct gaaccggacc ctcacgccac
      901 cggcgacgag gctggcgagg gccaacgcct cgcccgccgc ccagtcgagg gggacctcgc
      961 cgtcggccat ggcctcgcgc gtcttgagct gccgcgcgat cttcgagttc agcttgaagt
     1021 cggccggcac ctgcgtgagg agcctgagcc accgcttggc gtcgcccttg gcgatccgca
     1081 ggaactgagt cggcgacggc aaggccagc
//
LOCUS       DAOQYE010000908         1109 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_176813_length_1109_cov_1.989564, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000908 DAOQYE010000000
VERSION     DAOQYE010000908.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_176813_length_1109_cov_1.989564"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..615)
                     /locus_tag="PKN52_12155"
     CDS             complement(<1..615)
                     /locus_tag="PKN52_12155"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNR00751.1"
                     /translation="MTVFLARRLPRSLLTLAFIVTLVFVGTRLTGDPTMWLLPDDAPP
                     QARASMRAALAVDKPIPEQYWRYLQGLARGDFGNSFYERRPVTQVFAERLGPTLRLSG
                     LALVLSILIGVPVGIMTALHRNSVLDRTLMSLSFTAYAIPNFVLGIMLILIFSLVLRW
                     LPSGGYGDWRHYLMPVFTLGASHAALIARLTRSSMLEVLNEDYVR"
     gene            complement(612..>1109)
                     /locus_tag="PKN52_12160"
     CDS             complement(612..>1109)
                     /locus_tag="PKN52_12160"
                     /inference="COORDINATES: protein motif:HMM:NF028178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HNR00752.1"
                     /translation="LGAFGGASEKVASEVLQRFGYVESGAYIAKQAPFDEMITMLVDG
                     ELDLVFFLSSSRNSALIEFAIARELRFLSIDPPVLRELVKDHPYWQASRIEAFSYPHQ
                     DVPISTLRVPMTLITHSAVSEADVYSITRVLHAENVLLNPESAPGLDGLPQHPGAARY
                     WRDRR"
BASE COUNT          223 a          353 c          360 g          173 t
ORIGIN      
        1 gcgcacgtag tcctcgttga gcacctcaag catcgacgac ctggtcaacc tggctatcaa
       61 agcggcgtgc gaggcaccga gcgtgaacac tggcatgagg tagtgccgcc agtcgccgta
      121 accgccgctg ggcagccatc tgagcaccag actgaagatc aggatgagca tgatgccgag
      181 cacgaagttg gggatcgcgt acgccgtgaa gctcaacgac atgagcgtgc gatcgagcac
      241 gctgttccgg tgaagggccg tcatgatccc cacgggaacg ccgatcagga tcgagaggac
      301 caacgccagg ccactgagcc gcagcgtggg accgaggcgc tccgcgaaga cctgcgtcac
      361 gggacgccgt tcgtagaagc tgttcccgaa atcgccacgc gccaagcctt ggaggtaccg
      421 ccaatactgt tccgggatcg gcttgtcgac ggccagcgcc gccctcatgg aagcgcgcgc
      481 ctgtggcggc gcgtcgtctg gcaagagcca catggtgggg tctcccgtca ggcgcgtccc
      541 gacgaagacc agcgtgacga tgaacgcaag cgtcagaagc gacctgggga gcctccgcgc
      601 caggaagacc gtcatcgcct gtcccgccag tagcgagccg ctccggggtg ttgtggcaac
      661 ccgtcgagtc ccggcgccga ctccgggttc aacagcacgt tctctgcatg cagcacgcgc
      721 gttatcgagt agacgtccgc ttcggagacc gcgctatgcg taatgagggt catcggcacc
      781 cgcaacgtcg agatcgggac gtcttggtga gggtaagaga acgcctcgat gcggctggcc
      841 tgccagtacg gatggtcctt gaccaactcg cggagcacgg gcgggtcgat ggacaagaac
      901 cgcagttcgc gcgcgatggc gaactcgatc agcgcggagt tcctggacga cgacaggaag
      961 aacaccaggt ccagctcgcc atcgaccagc atggtgatca tctcgtcgaa cggggcctgc
     1021 ttggcgatat acgcgccgct ctcgacgtaa ccgaaacgct gcagcacctc gctggccacc
     1081 ttctcgctgg cgcctccgaa cgctccgag
//
LOCUS       DAOQYE010000909         1108 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_177111_length_1108_cov_1.894587, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000909 DAOQYE010000000
VERSION     DAOQYE010000909.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1108
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_177111_length_1108_cov_1.894587"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="PKN52_12165"
     CDS             <1..295
                     /locus_tag="PKN52_12165"
                     /inference="COORDINATES: protein motif:HMM:NF016145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HNR00753.1"
                     /translation="ALFAMSDARYVVSVRLHGCILAARLGIGFAGLSYDPKVKGFLSQ
                     AAAPAFTAPVDERALVNLVRAAPPAAAHAVDHLTRLADEGLDWLAATLTGGAV"
     gene            382..>1108
                     /locus_tag="PKN52_12170"
     CDS             382..>1108
                     /locus_tag="PKN52_12170"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNR00754.1"
                     /translation="MPFRTLPTVMSAWSSASGALRALVCSGLLLAVGAAHAQADFAQM
                     RDLLTQGYYNSAARLNGPELIAHHPDSAEAHFLYAKALYLTGNPDKAQVELDRAIELA
                     GDSDPAVVNLQGLLRAAAGDPVGALRALQNAFLRSRDYAFAMDWGRVAWQAARFDEAV
                     SAFDAAATTTRGQRELWPYIDKGRLFMFVGRLQDAISAFNAAIDVFEATDNGEARPGS
                     PAYVEAYYRMGEAYEQLGDLERAE"
BASE COUNT          150 a          392 c          380 g          186 t
ORIGIN      
        1 ggcgctcttc gccatgtccg acgcgcgtta cgtcgtgtca gtgcgtctgc acggctgcat
       61 cctcgccgcg cgactgggga tcggcttcgc tgggctgtcc tacgatccca aggtgaaggg
      121 gttcctctcg caggccgccg ccccggcttt cacggccccg gtggatgagc gcgccctcgt
      181 gaacctcgta agagccgctc ccccggccgc ggcgcatgcc gtcgatcacc tgacgcgcct
      241 ggcggacgaa gggctcgact ggttggcggc cacgttgacc gggggtgcag tgtgaacccg
      301 tggtcggtcc gccgaccggg cacccggccg cccggcccgg gcgcggcccc ttctcatagc
      361 tccttgaggt acccttgccc cgtgccgttc aggacgttac ccacggtcat gagcgcctgg
      421 tcgtccgcgt ccggcgcgct gcgcgcgctg gtctgctctg gtctgctgtt ggctgtaggc
      481 gccgcgcacg cccaggcgga cttcgctcag atgcgagacc ttctcactca gggctattac
      541 aactcggcgg cgcgtttgaa cggccccgag ttgatcgcgc accatcccga cagcgcggaa
      601 gctcacttcc tctacgccaa ggcgctctac ctcaccggca acccggacaa ggcgcaggtc
      661 gagttggacc gtgcgatcga actggccggc gactccgacc ccgccgtcgt caacctgcag
      721 ggtctgttgc gggccgcggc cggcgacccg gtcggcgcgt tgcgcgcgct ccagaatgcg
      781 ttcctgcgca gccgcgacta cgcgttcgcc atggactggg gccgggtggc gtggcaggcc
      841 gcgcgtttcg atgaggccgt gtcggcgttc gacgcggcgg cgacgacgac gcgcgggcag
      901 cgcgagctat ggccctacat cgacaaaggc aggctgttca tgttcgtcgg acggctccag
      961 gacgccatct cggccttcaa tgccgccatc gacgtgttcg aggcgaccga caacggcgag
     1021 gcccggcccg gcagcccggc ttacgtggag gcctactacc ggatgggcga ggcgtacgag
     1081 cagcttggcg acctagaacg cgccgagg
//
LOCUS       DAOQYE010000910         1105 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_177801_length_1105_cov_3.703810, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000910 DAOQYE010000000
VERSION     DAOQYE010000910.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_177801_length_1105_cov_3.703810"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /gene="rplR"
                     /locus_tag="PKN52_12175"
     CDS             complement(<1..319)
                     /gene="rplR"
                     /locus_tag="PKN52_12175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015302640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HNR00755.1"
                     /translation="MNVSVRKERRANRTRRRLKRPVHTERLRLSVFRSAKHIYAQVID
                     DEQGRTVAEANSKTLAPKGNKTEQAKEVGKLLAERAVAAGVKSVVFDRGSFRYHGRIK
                     ALAE"
     gene            complement(343..885)
                     /gene="rplF"
                     /locus_tag="PKN52_12180"
     CDS             complement(343..885)
                     /gene="rplF"
                     /locus_tag="PKN52_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HNR00756.1"
                     /translation="MSRIGRSPIRLPKGVETKLTEPGKVSVKGPKGSLDVVFSPRLEV
                     KEEAGSLNVARPSDNRDDRAQHGLARTLIANAVTGVTEGYEKKLEIHGVGFRCANKGT
                     NLELTIGYSHPVVVEAPQGITIQATEATRITVSGIDKQLVGQVAANIRALRPPDSYHG
                     KGIRYAGETVRLKPGKSAAR"
     gene            complement(885..>1105)
                     /gene="rpsH"
                     /locus_tag="PKN52_12185"
     CDS             complement(885..>1105)
                     /gene="rpsH"
                     /locus_tag="PKN52_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013858092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HNR00757.1"
                     /translation="RIGLKYGHKRSPVIHSIKRVSTPGRRAYAKAEHVPVVRGGLGLA
                     IISTSRGLLADREARRNNVGGEIVCEVW"
BASE COUNT          144 a          339 c          406 g          216 t
ORIGIN      
        1 cttcagcgag ggccttgatg cgaccgtggt acctgaacga cccccggtcg aagacgacgc
       61 tcttgacacc ggcggcgacg gcgcgctcgg cgaggagctt gccgacttcc ttggcttgct
      121 ccgtcttgtt gcccttgggg gccagcgtct tgctgttcgc ttccgccacg gtacggccct
      181 gctcgtcgtc gatgacttgg gcgtagatgt gcttggcgct gcggaacacg gagaggcgca
      241 ggcgctcggt gtgaacgggg cgcttgaggc gcctgcgggt gcggttggcg cggcgttcct
      301 tgcgtacgga tacgttcatg ctcatgatct accctgcccc gctcaacgcg ccgcggactt
      361 gccgggcttg agtcggacgg tttctcccgc gtacctgatg cccttaccgt gataggagtc
      421 gggtggacgc aaggcgcgga tgttcgcggc tacctggccg acgagctgct tgtcgatgcc
      481 gctcacggtg atgcgcgtgg cctctgtcgc ctggatggtg atgccctggg gggcttccac
      541 cacgaccggg tgcgagtagc cgatggtgag ctcgaggttc gtgcccttgt tggcgcagcg
      601 gaagccgacg ccgtggatct cgagcttctt ctcgtaccct tccgtcacgc cggtgacggc
      661 gttggcgatg agggtacggg cgaggccgtg ctgggcacgg tcgtcgcggt tgtcgctggg
      721 gcgggcgacg ttgagagagc cggcctcctc cttgacttcg aggcgcgggc tgaacacgac
      781 gtcaaggctg cccttggggc ccttgaccga caccttgcct ggttcggtga gcttcgtctc
      841 gacgcccttg ggcagcctga tcggcgagcg accgatgcgc gacatcacca cacctcacag
      901 acgatctcgc caccgacgtt gttgcggcga gcttcgcggt cggcgaggag gccgcgcgag
      961 gtggagatga tggcgaggcc gaggccccca cgcacgaccg gcacgtgctc ggccttggcg
     1021 taggcgcggc ggcccggggt ggagacgcgc ttgatggagt ggatgacggg cgagcgcttg
     1081 tggccgtact tgaggccgat gcgca
//
LOCUS       DAOQYE010000911         1105 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_177968_length_1105_cov_1.994286, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000911 DAOQYE010000000
VERSION     DAOQYE010000911.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_177968_length_1105_cov_1.994286"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="PKN52_12190"
     CDS             complement(<1..289)
                     /locus_tag="PKN52_12190"
                     /inference="COORDINATES: protein motif:HMM:NF015377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
                     /protein_id="HNR00758.1"
                     /translation="MQSQRNDCGPAVVATLAAWSGLALDLASVTAAAQLGPDGLSLGE
                     FARLADRFGFSGAWYRVAAADLVGLQTPFVAHLTLPGTVPLGHLVTARTATV"
     gene            complement(679..>1105)
                     /locus_tag="PKN52_12195"
     CDS             complement(679..>1105)
                     /locus_tag="PKN52_12195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00759.1"
                     /translation="AAVVELAKRGKVAFVGDGVNDAAALASADVGVAMGVAGSDAALE
                     AADVALLSDDLARLPEAFALAKRANMVIRQNLAFALGIMVLMVVVTVFFHLPLPLGVI
                     GHEGGTILVVTNGLRLLGFAPQAPAKAAHPATSLSTTAG"
BASE COUNT          169 a          383 c          418 g          135 t
ORIGIN      
        1 cgaccgtcgc cgtgcgcgcg gtcacgaggt gccctagcgg caccgtgccg gggagggtca
       61 ggtgagcgac gaacggcgtc tgaaggccga ccaggtccgc cgccgcgacg cggtaccagg
      121 cgcccgagaa cccgaagcgg tccgcgaggc gggcgaactc gcccaggctc agaccgtcag
      181 gaccgagttg cgccgcggcc gtcacggaag cgaggtcgag cgccagcccc gaccacgcgg
      241 ccagcgtggc gacgacggcg ggcccgcagt cgttgcgctg cgactgcatg acgacgggta
      301 ccgactccag gaggtatggc gcggcgttcc aaacccggct ggcatgagct gcgatggcga
      361 tagggagggc gacgacggcc gcggcagcca ggctagcgac taacagcgcg atggcgcggc
      421 gcgagccggc gcctccgagc ggcgcaagac gcgccgaacc gcgcggcgag ccgggccgcc
      481 cggcgaacgc attgcgggta tgggtgtctc cggatgccgt cctccccatc gcgggttcct
      541 ccttgcgagg aggctagggg aacggcagtg tgggcacacc cgccgagttg gctcgagccg
      601 tgtcggtcta ggcggttgtc cggtcgcggc tcacgtgacg gcgccgcgtc tctggcccgg
      661 ccgaaggcgg accggccctc agccggcggt ggtcgagagg ctcgtcgccg ggtgcgcggc
      721 cttggcgggc gcttgtggag cgaaccccaa cagtctcagg ccgttggtga cgaccaggat
      781 ggtgccaccc tcgtgcccga tcacgcccag gggcagcggg aggtggaaga agacggtgac
      841 gacgaccatc aggaccatga tcccgagcgc gaaggcgagg ttctggcgga tgaccatgtt
      901 ggcgcgcttc gccagcgcga acgcttccgg gagccgcgcc aggtcgtccg agagcagcgc
      961 gacgtcggcc gcctccagcg cggcgtcgct gccggccaca cccatggcca caccgacgtc
     1021 ggcgctggcc aacgcagcgg cgtcgttgac cccgtcgccg acgaacgcca ccttgccgcg
     1081 cttggccagt tccactaccg ctgcg
//
LOCUS       DAOQYE010000912         1104 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_178099_length_1104_cov_3.542421, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000912 DAOQYE010000000
VERSION     DAOQYE010000912.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1104
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_178099_length_1104_cov_3.542421"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            89..>1104
                     /gene="coxB"
                     /locus_tag="PKN52_12200"
     CDS             89..>1104
                     /gene="coxB"
                     /locus_tag="PKN52_12200"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HNR00760.1"
                     /translation="MSPAASIAREQHGLFMWTWYLSIPVMVGVFGALFYVLIKFRRRR
                     DDEMPDQSHGNPMTEVILTIVPVIIVIMVAVPSVRSIFRTQTNVVATSDDVVVHVTGY
                     QWWWKFEYPQYGITTANEMHIPAGKRVILQLNSGDVVHAFWVPRLAGKVDLIPNQDNR
                     LWFDTDAEITDEDNGFDVYTGQCAELCLGAHAYMRFRVVVDEQDRFDDWVADFQQPEV
                     VATATDPLVAQGRQLVATKGCIGCHNIGNFAEGVKYGQPQYPDLTNFGLRHTVGAAIL
                     DATPENVATWVRDPQEVKPGTRMPTLWQATDPNRDQEAAAIAAYLLSLGVEDGAQAMA
                     QATTG"
BASE COUNT          199 a          377 c          347 g          181 t
ORIGIN      
        1 gcccatgagg tggttgtggc gggcgttgcc cgtcgccgtt ctcgcgccgt tattggcagc
       61 gtgctcgctc gacggagtcc agtcgatcat gagccccgcg gccagcatcg cgagggaaca
      121 acacggtcta ttcatgtgga cgtggtacct gagcatcccc gtcatggtgg gtgtcttcgg
      181 ggcgctcttc tacgtcctca taaagttccg gcgacgcagg gacgacgaga tgccggacca
      241 gtctcacggc aacccgatga ccgaggtgat cctcaccatc gtgcccgtca tcatcgtcat
      301 catggtcgcc gtgccgtccg tgcggtccat cttccgcacc cagacgaacg tggtggccac
      361 cagcgacgac gtggtcgtgc acgtgaccgg ttaccagtgg tggtggaagt tcgagtaccc
      421 gcagtacggc atcaccacgg ccaacgagat gcacatcccg gccggcaagc gcgtcatcct
      481 ccagctcaac tccggcgacg tggtgcacgc cttctgggtc ccgcgcctcg cgggcaaggt
      541 cgacctcatc cccaaccagg acaaccgtct gtggttcgac acggatgccg agatcacaga
      601 cgaggacaat ggcttcgacg tctacaccgg ccagtgcgcg gagttgtgcc taggggcgca
      661 cgcctacatg cggttccggg tcgtggtgga cgaacaagac aggtttgacg actgggtcgc
      721 tgacttccag cagccggagg tcgtggccac ggccaccgac ccgctcgtgg ctcagggccg
      781 ccagctcgtc gccacgaagg gctgcatcgg ctgccacaac atcggcaact tcgccgaggg
      841 cgtgaagtac ggccagcccc agtaccccga tctcaccaac ttcggcctgc gccacacggt
      901 gggggccgcc atcctggacg ctacgcccga gaacgtcgcc acctgggtgc gcgacccgca
      961 ggaagtcaag ccgggcacgc gcatgccgac cttatggcag gccaccgacc ccaaccgtga
     1021 ccaggaggcg gcagccatcg ccgcctactt gctgagcctc ggcgtcgaag acggtgccca
     1081 agccatggcc caggcaacga ctgg
//
LOCUS       DAOQYE010000913         1103 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_178570_length_1103_cov_1.853053, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000913 DAOQYE010000000
VERSION     DAOQYE010000913.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1103
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_178570_length_1103_cov_1.853053"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            195..>1103
                     /locus_tag="PKN52_12205"
     CDS             195..>1103
                     /locus_tag="PKN52_12205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00761.1"
                     /translation="MPVSFPDDPRVVNGEWELSITGLGYAVERAVLDQAGRRLLLSDE
                     TANLWLYERSDNIGWVEADPGPYRTFRSSPYNELLDSLVTVTRSGMTATATAISLADG
                     TTSQRKFDLPAGEPELLTVGSGSLFAVFPPGEVQRQVHWWDLASGEYGGSLSVPPAND
                     GVRRSSGGRFLSFWEVRGGTVYVLDTSDPARLRSFGLGICRSGGVGEGSADGRWFLGE
                     DCNENLRVLDLHEESPTWRNLGVKHITPIRFARGADAIVWQDTRGRVHSLDLLAGGSR
                     TLAEVGAPPDWFWHAQRTLTVDERNGL"
BASE COUNT          154 a          404 c          397 g          148 t
ORIGIN      
        1 cgcgccggac gggcggcagc acgagaaact cgccgccgcg gtgggccgga ccgactgagc
       61 ggtaggcgcg acaggttcct cagtcacctc cgcgtgaccg gagtcgcgcg accgcgccga
      121 ggcggttcgc cgcgctggcc ctcccgctgg tcgcggtagc ggtgctcgca actgcctgcc
      181 ggacggagcg ccccgtgccg gtctcgttcc cggacgaccc gcgcgtggtg aacggcgagt
      241 gggagctctc gatcaccggg ctcggctacg ccgtggagcg cgccgtgctc gatcaggcgg
      301 gcagacgcct cctcctgagc gacgagaccg ccaacctgtg gctgtacgag cgctcggaca
      361 atatcgggtg ggtcgaggcc gacccggggc cataccgcac gttccgctcg tcgccgtaca
      421 acgagctgct cgactcgctc gtgaccgtga cgaggtccgg catgacggcg accgccacgg
      481 ccatctcgct cgccgacggc accacgagcc aacggaagtt cgacctgccg gccggcgagc
      541 ccgagctcct aaccgtcggg tccgggagcc tgttcgcggt cttcccgccc ggtgaggtgc
      601 agcgccaggt ccactggtgg gacctggcga gcggcgagta cggcggcagc ctgagcgttc
      661 cgcccgccaa cgacggcgtc cgccgcagct ccggcggccg gttcctgagc ttctgggagg
      721 tgcgcggcgg gacggtgtac gtgctcgaca caagcgaccc ggcgcgcctg cgcagcttcg
      781 gcctcggcat ctgccgctcc gggggcgtcg gcgagggcag tgccgacggc cgctggttcc
      841 tcggcgagga ctgcaacgag aacctccgag tcctcgacct gcacgaggag tcgccgacgt
      901 ggcggaacct cggcgtcaag cacatcacgc ccatccgctt cgccaggggc gccgacgcga
      961 tcgtctggca ggacacgagg ggccgcgtcc actccctcga cctactcgcc ggcgggagcc
     1021 ggaccctggc ggaggtcggc gcgccccccg actggttctg gcatgcacag cggaccctga
     1081 cggtcgacga gcgcaacggc ctc
//
LOCUS       DAOQYE010000914         1101 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_178998_length_1101_cov_2.631931, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000914 DAOQYE010000000
VERSION     DAOQYE010000914.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_178998_length_1101_cov_2.631931"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /gene="gmk"
                     /locus_tag="PKN52_12210"
     CDS             complement(<1..564)
                     /gene="gmk"
                     /locus_tag="PKN52_12210"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HNR00762.1"
                     /translation="MNEGAEGAAEASRSRVGRRGVLFVMTGASGVGKDTIRGAAAPFL
                     SDVAYSISATTRARRPGEEHGVQYYFVSTTEFEAMIASDALLEYADYVGDHYGTPKAP
                     VTEALERGQDVLLELELVGARQVKRRMPEAVMLFIAPPSLRELERRLRGRGTDSEDKI
                     QKRLARAREEIRALREFDYLIVNDELER"
     gene            758..>1101
                     /gene="pyrG"
                     /locus_tag="PKN52_12215"
     CDS             758..>1101
                     /gene="pyrG"
                     /locus_tag="PKN52_12215"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178433.1"
                     /note="CTP synthase; cytidine triphosphate synthetase;
                     catalyzes the ATP-dependent amination of UTP to CTP with
                     either L-glutamine or ammonia as the source of nitrogen;
                     in Escherichia coli this enzyme forms a homotetramer;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthetase"
                     /protein_id="HNR00763.1"
                     /translation="MDRKYVFVTGGVVSSLGKGITTSSIGALLRARGYRVTAVKIDPY
                     VNVDAGTMRPYEHGEVFVTDDGAETDLDIGTYERFLDVDLGRDNSITTGQVYLRVIEQ
                     ERFSRSQFLRPP"
BASE COUNT          177 a          393 c          356 g          175 t
ORIGIN      
        1 ccgctccagc tcgtcgttga cgatgaggta gtcgaactcc cgtagagccc ttatctcttc
       61 gcgggcgcgc gccagtcgct tctggatctt gtcctcgctg tccgtaccgc gcccgcgcaa
      121 ccggcgctcc agctcccgca gcgacggggg cgcgatgaag agcatgaccg cctccggcat
      181 gcgccgcttc acttggcgcg ccccgacgag ctcgagctcg agcagcacgt cctggccgcg
      241 ctcgagcgcc tccgtcaccg gcgccttcgg ggtgccgtaa tggtcgccga cgtaatcggc
      301 gtactcgagc agggcgtccg acgcgatcat agcctcgaac tccgtggtcg acacgaagta
      361 gtactggacg ccgtgctctt cgccggggcg ccgcgcccgc gtggtagcgg atatcgagta
      421 ggcgacgtcc gacaggaagg gggcggctgc gcccctgatg gtgtccttcc cgacgccgga
      481 cgcgccggtc atgacgaaca agacccccct gcgacctacg cggctccgcg acgcttccgc
      541 cgcaccttca gcaccttcgt tcaccctcgg tcaacgcctc ctgagcgtct cgtgtccaac
      601 agtcgatgcg cgcggccaca cgcgccaaac cgagcgcggc gggatgagcg cgactgtacc
      661 atgctcgccg gaacggccct tggcggcccc cgccaccgcg catcctcacg ggggcagcac
      721 cggcttgggt tacgattgag tcccgggaag gtggatcatg gaccggaagt acgtgttcgt
      781 cactgggggt gtggtctcca gtctcgggaa gggcataacg acctcgagca tcggagcgct
      841 gctgcgcgcc cgcggctacc gggtcaccgc cgtcaagata gacccgtacg tcaacgtcga
      901 cgcgggcacg atgcggccgt acgagcacgg cgaggtgttc gtcaccgacg acggggcgga
      961 gacggacctc gacatcggca cctacgaacg ctttctggac gtcgacctgg ggcgcgacaa
     1021 cagcatcacg acgggccagg tctacctccg cgtcatcgag caggagcgtt tcagccgcag
     1081 ccagttcctc cgcccgcccg a
//
LOCUS       DAOQYE010000915         1101 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_179062_length_1101_cov_2.186424, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000915 DAOQYE010000000
VERSION     DAOQYE010000915.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_179062_length_1101_cov_2.186424"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="PKN52_12220"
     CDS             <1..436
                     /locus_tag="PKN52_12220"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNR00764.1"
                     /translation="GYLLTYPLLVGSPWGVPVAHALLAFPFVTRTLLPAYRSQPPVLA
                     AAAATLGAGPLRTLWRVDLPLLRPAFLTAAAFAFAVSMGEFGATLLLVRPEYATLPVA
                     IFDRLGRPGAANYGSALALAVVLMGLTAAVMLLLQRRTRSEF"
     gene            583..>1101
                     /locus_tag="PKN52_12225"
     CDS             583..>1101
                     /locus_tag="PKN52_12225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNR00765.1"
                     /translation="MSLRAEGVTKRYGNVTAVDGASFELPTGTTLALLGPSGCGKSTL
                     LRLIAGLEAPDAGVLALGGVDLASTPPAKRRFGMVFQDYALFPYLDVAGNVAFGLVEA
                     RMPAPLRAARVAELLELVGLAGFERRKVDELSGGQRQRVALARALAPAPRLLLLDEPL
                     SNLDAKIRQQVRA"
BASE COUNT          129 a          405 c          394 g          173 t
ORIGIN      
        1 cggctacctg ctcacctacc ccctgctcgt cggctcgccc tggggcgtgc ccgtggcgca
       61 tgcgcttctc gccttcccgt tcgtgacgcg cacgctgctg cccgcgtacc gctcgcaacc
      121 gccggtactg gcggccgcgg ccgccacgct cggtgccggg ccgctccgca cgctctggcg
      181 cgtcgacctc ccgctgctcc gcccggcgtt cctcacggct gccgccttcg cgttcgctgt
      241 gtcgatgggc gagttcggag ccacgctgct cctggtgagg cccgagtacg cgaccctgcc
      301 tgtcgccatc ttcgaccgcc tcgggcgacc gggcgcggcg aactatggct cggcgctggc
      361 gctcgccgtc gtcctgatgg ggctgacggc cgccgtcatg ctcctcctcc agcggcgtac
      421 caggagcgag ttctgagggg agcggcggcg gcgcgaaccg tcatggcgcc ggcgtccgag
      481 cccggcgggg cgtcggagca gctcgacagg tgctcgaacc gggcaaccgc ttatgatgcc
      541 caggatggga atccgccgtc cccaacgcac aggtcgtgcc gcatgagcct gcgggcggaa
      601 ggtgtaacga agcgctacgg gaacgtgacc gcggtcgacg gggcgtcctt cgagcttccg
      661 acagggacca cgctcgccct gctcggaccg agcgggtgcg gcaagagcac gttgctgcgg
      721 ctgatcgccg gactggaggc gccagatgcc ggcgtgctgg cgctcggcgg cgtcgacctg
      781 gcgtcgacgc caccggccaa gcggcggttc ggcatggtgt tccaggacta cgcgctcttc
      841 ccctacctgg acgtggcagg caacgtcgcg ttcgggctcg tggaggcgcg catgccggcc
      901 ccgctgcgcg cggcgcgcgt ggcggagttg ctggaactcg ttggcctcgc cgggttcgag
      961 cgtcgcaagg tcgacgagct cagcggcggt cagcggcaac gcgtggcgct cgctagggcg
     1021 ttggcgcccg caccgcgcct gctgctcctc gacgagcccc tctccaacct ggacgccaag
     1081 atccgccagc aggtgcgcgc c
//
LOCUS       DAOQYE010000916         1100 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_179390_length_1100_cov_1.797129, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000916 DAOQYE010000000
VERSION     DAOQYE010000916.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_179390_length_1100_cov_1.797129"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..604
                     /locus_tag="PKN52_12230"
     CDS             <1..604
                     /locus_tag="PKN52_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNR00766.1"
                     /translation="PVITKALIAVSDFLRYQWYILFGLIIAIVVGLVLFYRTKNGRRI
                     IDGFTLRMPIVGTLVQKTAIASFSNTFGLLLRSGVNIIESIDITKGTAGNAIVEDILD
                     ETKENVQRGEQISTTLIKYPGVFPPMVSSMISIGEETGAVDHMLQKVADFYEREVDEA
                     VDSLTAALEPMLIVFLGVIVGFIVAGMFLPMFSIIGQLSQ"
BASE COUNT          194 a          347 c          331 g          228 t
ORIGIN      
        1 gcccgtcatc accaaggcgc tcatcgccgt ctccgacttc ctgcgttacc agtggtacat
       61 cctcttcggc ctgatcatcg cgatcgtagt cgggttggtc ctcttctacc ggacgaagaa
      121 cggcaggcgc atcatagacg gcttcacgct gcgtatgccc atcgtcggca ccctcgtgca
      181 gaagaccgcc atcgcgagct tctccaacac gttcggcttg ctcctgcgct ccggcgtcaa
      241 catcatcgag tccatcgaca tcacgaaggg cacggccggc aacgccatcg tcgaggacat
      301 cctcgacgag acgaaggaga acgtgcagcg cggcgagcag atcagcacca cgctcatcaa
      361 gtacccgggc gtgttcccgc ccatggtctc gtcgatgatc tcgatcggcg aggagacggg
      421 cgccgtcgac cacatgctcc agaaggtcgc agacttctac gaacgcgagg tcgacgaggc
      481 ggtcgacagc ctcaccgccg cgctcgagcc catgctcatc gtgttcctcg gcgtcatagt
      541 cggcttcatc gtggccggca tgttcctgcc gatgttctcg atcatcgggc agttgagcca
      601 gtaggcgttg gtcagggccg gcgttgtaag aatggtacag ttggccttgt aaaaacggca
      661 cagtagatcg tgtaagaaca aggcggctgc cgattacctt ccgcgcatag ggagcagttg
      721 gggtaacggc gctggcttgc gcatctctag ggttcgccac tcgtttcgga ggaggtctgc
      781 tgtaggtcga tgggttccct tggaccgacc acctgctcag ccgccatatt cttcggagac
      841 cctctgttcg gagccatagt tatcgcgtaa cttcggctcg ctctcgaggg tgtctgtact
      901 ccatcgtgag ggccgccacc ctgcatcatg aggcttcggc ccgtacctat gcgggcgtgc
      961 gcatgggcct gatccctgcg caccggtcgc tggcgacgat ggtgtcatgc cgacgaatgg
     1021 tgtcatgccg acgagccgcc ctcgaggttc tcgagccacg gtcatcgcgt aacctcgact
     1081 cgcctgcgag ggtgctggtg
//
LOCUS       DAOQYE010000917         1099 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_179507_length_1099_cov_2.806513, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000917 DAOQYE010000000
VERSION     DAOQYE010000917.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1099
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_179507_length_1099_cov_2.806513"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..733)
                     /locus_tag="PKN52_12235"
     CDS             complement(<1..733)
                     /locus_tag="PKN52_12235"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNR00767.1"
                     /translation="MTTAPTPRQAPRRRLAVILPALLATLLTFTTATAQTATPTELVV
                     AVTAIGNTLDAAVANFTNTTIAMNHVYDRIVDFDENFEFAPGVATSWEFVDATTFKVT
                     LGDGFVFHNGAPLTAQDVVFSVERLREVPRLASVMNNIASTEATGASEVTIKLVEPNS
                     STIRELMAAAHVLNQAYSETPGADFANKPIGTGPYIVKNFVPGDRLELEAWADYPFAA
                     PAIPRIVFRTIPEDANRYIAAETGEA"
     gene            complement(878..>1099)
                     /locus_tag="PKN52_12240"
     CDS             complement(878..>1099)
                     /locus_tag="PKN52_12240"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HNR00768.1"
                     /translation="QTRWMALEFKRLIAPTIGFAPGDGPSTEWIHGLKDYREFPNVTA
                     GLLARGYSDVDAAKVIGGNWLRVLREVCG"
BASE COUNT          161 a          335 c          379 g          224 t
ORIGIN      
        1 gcgcctcgcc cgtctcggcg gcgatgtagc ggttggcgtc ttccgggatg gtcctgaaca
       61 cgatgcgggg gatggcgggc gcggcgaacg ggtagtcggc ccacgcctcc agctcgagcc
      121 tgtcgcccgg cacgaagttc ttcacgatgt acgggcctgt gccgatcggc ttgttcgcga
      181 agtcagcgcc cggagtctca ctgtacgcct ggttgaggac gtgcgctgcg gccatcagtt
      241 cgcggatggt cgagctgttc ggctccacca gcttgatggt cacctcggac gctcccgtcg
      301 cttccgtcga ggcgatgttg ttcatgaccg aggcgaggcg cggtacctcg cgcaggcgct
      361 cgaccgagaa cacgacgtcc tgggccgtga gcggcgctcc gttgtggaac acgaagccgt
      421 cccccagcgt cactttgaag gtcgtggcgt ccacgaactc ccagctcgtg gctacgccgg
      481 gcgcgaactc gaagttctcg tcgaagtcga cgatccggtc gtagacgtgg ttcatggcga
      541 tagtggtgtt ggtgaagttc gctaccgccg cgtccagcgt gttgccgatg gcggtgacgg
      601 ccaccaccaa ctcagtgggc gtggcggtct gagcggtcgc ggtcgtgaac gtgagcaggg
      661 tggcgaggag ggccggcagg atcacggcga gccgcctgcg tggtgcttgt ctgggggtgg
      721 gggctgtcgt catgttctca gggctccgtt tcttgaggtc tacgttcttg aggtctacgt
      781 tcttgaggtc tacgtcgtga gatgtctcga ggggcctcag ggcgcttcga ggaagctcgc
      841 gtggtggtgg ttcattcccg agcatcggct ctgatcatca gccgcacacc tcccgcagca
      901 ctctcaacca gttgcctccg atgaccttgg cggcgtccac atcgctgtac ccccgggcga
      961 gcaggccagc ggtgacgttg gggaactcgc ggtaatcctt tagaccgtgg atccactcgg
     1021 tcgagggccc gtcgcccggc gcgaacccga tagtcggagc tatcaggcgc ttgaactcca
     1081 gcgccatcca cctagtctg
//
LOCUS       DAOQYE010000918         1098 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_179784_length_1098_cov_2.658677, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000918 DAOQYE010000000
VERSION     DAOQYE010000918.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1098
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_179784_length_1098_cov_2.658677"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="PKN52_12245"
     CDS             <1..460
                     /locus_tag="PKN52_12245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HNR00769.1"
                     /translation="LYRMRVMRDDPRGMAIQRQRVLAVHRHVDLAAMAAATRYLLGTH
                     DFSTFATQETRTTVRTVHLCELREERGEVRLHIAADGFLRNMVRTIVGTLLWVGKGKL
                     TAPDMAELLAARDRRRAGHNVGPQGLYFVEAGYTPWDAEASERAVRDLVV"
     gene            484..981
                     /locus_tag="PKN52_12250"
     CDS             484..981
                     /locus_tag="PKN52_12250"
                     /inference="COORDINATES: protein motif:HMM:NF020946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1999 family protein"
                     /protein_id="HNR00770.1"
                     /translation="MTIRIRPLVEGDLERLAELDDAYSTANDLERAVNLAALRFFERS
                     GHSFVAEAAGGSAAGAPGEGAGDAVGFLLAQAVWTGERPTVHAHRLVARGADAAGARS
                     ALARALVKSAYDAGVYDLVFRTPRADAALRTALAGEGYLQDDHVTLALVLGTRAAAWA
                     EARGG"
BASE COUNT          143 a          375 c          414 g          166 t
ORIGIN      
        1 cctctaccgg atgcgcgtga tgcgcgacga cccgcgcggc atggccattc agcgccagcg
       61 cgtgctggcc gtgcatcgcc acgtcgatct ggccgccatg gccgcggcga cccgctacct
      121 gctcggcacc cacgacttct cgaccttcgc cacgcaggag acgaggacga ccgtccgcac
      181 ggtccacctc tgcgagctgc gcgaggaacg gggcgaggtg cgcctccaca tcgcggcgga
      241 cggcttcctg cgcaacatgg tccgcaccat cgtcggtacg ctcctgtggg tggggaaggg
      301 gaagctgacg gcccccgaca tggccgagct gctggccgcg cgcgaccgta gacgggcggg
      361 tcacaacgtc ggcccgcagg gcctgtactt cgtcgaggcc ggctacacgc cttgggatgc
      421 cgaggcttcc gagcgcgccg tgcgagacct ggtcgtctga ggcgccttac gtatactcgg
      481 tgcatgacca tccgtatcag accgctcgtc gagggggacc tcgagcggtt ggccgagctg
      541 gacgacgcgt actcgaccgc caacgacctg gagcgggccg tcaacctcgc agccctacgg
      601 ttcttcgagc ggagcggtca ctcgttcgtg gccgaggcgg cgggcggctc cgccgctggc
      661 gctcccggtg agggcgcggg tgacgcggtc ggcttcctcc tggcgcaggc ggtctggacg
      721 ggcgagcggc cgacggtgca cgctcaccgc ctcgtggcgc gtggcgcgga cgccgccggc
      781 gcgcgttcgg ccctcgccag ggctctcgtc aagagcgcct acgacgccgg ggtctacgac
      841 ctggtgttcc gcacgccccg cgcggatgcc gctttacgca ccgctctggc gggtgagggc
      901 tacttgcagg acgatcacgt gacgctggcg ctggtcttgg gcacgcgcgc cgcggcctgg
      961 gcggaggcta gaggtggctg acgtcggcgc gagggtcctg atgggggtca gggggatgga
     1021 cacgctggag gcgcgcgagc gcgtcatggc cacgctgcgg ttcgtgccgg gggtgatgga
     1081 ggtcacgccc ggcgatgc
//
LOCUS       DAOQYE010000919         1097 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_180048_length_1097_cov_3.095010, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000919 DAOQYE010000000
VERSION     DAOQYE010000919.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1097
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_180048_length_1097_cov_3.095010"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(333..>1097)
                     /locus_tag="PKN52_12255"
     CDS             complement(333..>1097)
                     /locus_tag="PKN52_12255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HNR00771.1"
                     /translation="RRVTERYDVRTVDGQRAVLEELQGVLRPRDVFDPVASEMRRLVI
                     EHLGIDGLRLDEWLAAKSRRAPSQLEMRGMSAQRSELGQVRRLELEIVALLLLEPRHL
                     RRRLALVTEGLPDRDADSALVEFERHCVAHDYDADGVLDVYRRRDDGAVVLERLLAGD
                     DGGDGRMDVDGQLAKGLSRLRELGLEAQKVEGAARLRARRSELAQRLADADAGDPDLT
                     ALYLELQEIQGVLAARDAERRLRVPASYARQRKKRG"
BASE COUNT          163 a          412 c          344 g          178 t
ORIGIN      
        1 accgccgcct cgatgctggc cttggcgccg gccttggcct cctcgacctt gcggaccagg
       61 ttcttctcct gggtcacgag gtcttggagc agtcgttcac cttgtgcttc caaacggccc
      121 tccttctcgt cacgccaaca ctggcgccga tggactggcc cagatgttgt cggtatcgtc
      181 ctaggttacg tagcacacac aaaaaaacgc gacgttgcgg taaacaacgc ctctgacgcg
      241 ttcttctcca aacatatcag ggcacccagg gggtgtcaat caaggacgaa cgtcccgaac
      301 cggtcgccgg gcggccagac gtcgccctcg gttcagcccc gcttcttgcg ctgccgagcg
      361 tacgacgccg gaacccgcag ccgccgttcg gcatcgcgcg ccgccagcac gccctggatc
      421 tcctgcaact ccaggtagag ggcggtcagg tcggggtcgc cggcgtcggc gtcggccaaa
      481 cgctgggcca gttcgctgcg gcgagcgcgc aggcgggcgg cgccctccac cttctgcgcc
      541 tcgaggccga gctcgcgcag tcgggacagc cccttggcca gctggccgtc gacgtccatc
      601 cggccgtccc cgccgtcgtc cccggccagg aggcgctcaa gaaccaccgc gccgtcgtcg
      661 cgccggcggt acacgtcgag cacgccgtcg gcgtcgtagt cgtgcgcgac gcagtgacgc
      721 tcgaactcca cgagcgccga gtccgcgtcc ctgtcaggta gcccctccgt caccagcgca
      781 aggcgccttc gcaggtgtcg cggctcgagg aggaggagcg cgacgatctc gagctcgagg
      841 cggcgcacct gcccgagctc gctgcgttga gcgctcatgc cgcgcatctc cagttggctc
      901 ggcgccctgc gggacttggc ggccaaccac tcatcgagcc gcaagccgtc tatgccgagg
      961 tgctcgatca cgagcctccg catctcgctc gccacggggt cgaagacgtc gcgcgggcgc
     1021 aacacgccct gcagctcctc gagcaccgcc ctctgcccgt cgaccgtacg cacgtcgtag
     1081 cgctcggtca cgcgcct
//
LOCUS       DAOQYE010000920         1096 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_180335_length_1096_cov_3.173871, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000920 DAOQYE010000000
VERSION     DAOQYE010000920.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_180335_length_1096_cov_3.173871"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..933
                     /locus_tag="PKN52_12260"
     CDS             <1..933
                     /locus_tag="PKN52_12260"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNR00772.1"
                     /translation="QIGEMAVGGILLDMSSFATAAYLSDLSEQARLGYTFAGKLFGLP
                     MYVEGPALIVNDDLVPSVPATYEATMALAQQLTTADTFGFLFDIPNFYFSYGWLHTFG
                     GYVFGRDATGSLDANDVGLANEGAIAGGKALAALRYDLDLIPSGTNYDVANGLFVDGA
                     LGMIYTGPWAISQYQDVGLNVSVHPVPPLADGTPFSGFMGVQGVLVNSFTNLKVEAAN
                     FAKWLSRKDAQVSMARLSGRIPASVSALDEVMDDPIIAGFGAALLNSEPMPNIPAMGA
                     VWAPMGNALTVITEDASVDVAAVLEQAVDEIKGQ"
BASE COUNT          174 a          413 c          332 g          177 t
ORIGIN      
        1 cagatcggcg agatggccgt cggcggcatc ctgctcgaca tgagcagctt cgccaccgcc
       61 gcttacctct ccgacctgag cgagcaggcc cgtctcggct ataccttcgc gggcaagctc
      121 ttcggtctgc cgatgtacgt cgaaggtccc gccctcatcg tcaacgacga cctcgtcccg
      181 agcgtccccg ccacctacga ggcgacgatg gccctcgccc agcagctcac gacggccgat
      241 accttcggct tcctgttcga catccccaac ttctacttca gctacggctg gctccacacc
      301 ttcggcggct acgtgttcgg tcgtgacgcc accggctcgc tcgacgcgaa cgacgtcggt
      361 ctggccaacg aaggcgcgat cgccggcggc aaggccctcg ccgcgctgcg ctacgacctc
      421 gacctgatcc cgtccggcac gaactacgac gtcgccaacg gcctgttcgt cgacggcgcc
      481 ctcggcatga tctacacggg tccgtgggcg atcagccagt accaggacgt cggccttaac
      541 gtgagcgtcc acccggtgcc gccgctggcc gacggcacgc cgttctccgg cttcatgggc
      601 gtgcagggcg tgctcgtcaa ctcgttcacg aacctcaagg tcgaggccgc caacttcgcc
      661 aagtggctgt cccgcaagga cgcccaggtc tcgatggcca ggctctccgg gcgcatcccc
      721 gccagcgtct ccgcgctcga cgaggtcatg gacgacccga tcatcgccgg cttcggggcc
      781 gcgctgctca actccgagcc gatgccgaac atcccagcga tgggcgccgt gtgggccccg
      841 atgggcaacg ccctcaccgt catcacggag gacgcctccg tcgacgtcgc cgcggtactg
      901 gagcaggccg tcgacgagat caagggtcag tgaaccggcg cggcgcggcg cgatccgttc
      961 gcgccgcgcc gccgccctcg acccacgtag tggcggttga cgcgccacca cgacggccgg
     1021 accgatgaca ccgacttttc acggctagga atccctcgag ggctcctagc cgtcctgaat
     1081 caggcgggca tctaga
//
LOCUS       DAOQYE010000921         1095 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_180810_length_1095_cov_1.812500, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000921 DAOQYE010000000
VERSION     DAOQYE010000921.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1095
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_180810_length_1095_cov_1.812500"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..781
                     /locus_tag="PKN52_12265"
     CDS             <1..781
                     /locus_tag="PKN52_12265"
                     /inference="COORDINATES: protein motif:HMM:NF013408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HNR00773.1"
                     /translation="ALVGKASYGRYGPRIPLLAKFLDAAADLSVQVHPNDAYALREEA
                     GSGHLGKTEAWLMLECDPGASVLRGFVRDVTVEEARAALADDTFGELLNRVPVRTGDV
                     VVNPAGAVHAVGAGCFLFEIQQASDLTYRLYDYGRVGADGRQRELHIERALAVAELSG
                     GAYAPASTWPEADRWHRAVELPEFTLDVRRLEPGAQVAGATKPHSLELLVVTRGVAGL
                     NAAGHELTLGRGAAAVLPASLGAYALRGDADIVRAAVGEAA"
     gene            782..>1095
                     /locus_tag="PKN52_12270"
     CDS             782..>1095
                     /locus_tag="PKN52_12270"
                     /inference="COORDINATES: protein motif:HMM:NF014041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF84 family protein"
                     /protein_id="HNR00774.1"
                     /translation="MAAPTPNDAARLEALLFVALGSTSPAKVRATETALRTVHGRFVA
                     VTHVAAPSGVPAQPVGDEETYAGALARARGALALVPGARLGVGIEAGVTTGDPGARMY
                     TSA"
BASE COUNT          141 a          389 c          416 g          149 t
ORIGIN      
        1 cgccctcgtg gggaaagcct cgtacggacg ctacggtccg cgcatcccgc tcctcgccaa
       61 gttcctcgat gcggcggccg acctctccgt gcaagtgcac ccgaacgacg cctacgcgct
      121 gagggaggag gcaggcagcg gtcaccttgg caagaccgag gcctggctca tgctcgagtg
      181 cgacccgggc gcctcggtgc tgcgcggctt cgtccgcgac gtgacggtcg aggaggcgcg
      241 cgcggcgttg gcggacgaca ccttcggtga gctgctgaac cgcgtgcccg tgcgcaccgg
      301 cgacgtggtc gtcaacccgg ccggcgccgt gcacgccgtc ggagccggct gcttcctgtt
      361 cgagatccag caggccagcg acctcaccta tcgcctctac gactacggcc gtgtcggggc
      421 cgacggtaga cagcgcgagc tgcacatcga gcgcgcgctg gccgtcgccg agttgagcgg
      481 cggggcgtac gcgccggcga gcacgtggcc ggaggcggac cgctggcatc gcgccgtcga
      541 actgcccgag ttcacgctcg acgtgcgccg gctggagccg ggcgcgcagg tggcgggcgc
      601 gacgaagccg cactcgctcg agctcctggt cgtgacgcgc ggtgtggcgg gcttgaacgc
      661 ggcgggccat gagctgacgc tcggtcgtgg agccgcggcc gtcctgccgg cgagccttgg
      721 cgcttacgcg ctccgtggcg acgccgacat cgtgcgcgcg gcggtggggg aggccgcctg
      781 aatggccgca cccacaccga acgacgccgc tcgactggag gccttgctct tcgtcgccct
      841 cggctcaacc agtccggcca aggtcagggc gaccgagacg gcgctacgca cggtgcacgg
      901 gcgcttcgtg gccgtgaccc acgtcgctgc tccctccggc gttcccgccc agccggtggg
      961 cgacgaggag acgtacgccg gagccctggc gcgggcgcgc ggcgcgctcg cgctcgtgcc
     1021 gggggctcgg ctgggcgtgg ggatagaagc cggggtcacg actggcgacc ccggggcgcg
     1081 catgtacacg agcgc
//
LOCUS       DAOQYE010000922         1094 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_180908_length_1094_cov_3.106833, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000922 DAOQYE010000000
VERSION     DAOQYE010000922.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1094
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_180908_length_1094_cov_3.106833"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(115..522)
                     /locus_tag="PKN52_12275"
     CDS             complement(115..522)
                     /locus_tag="PKN52_12275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00775.1"
                     /translation="MPTFEDLDRLARAMPEADAHLSAAGRPEYRVRGKSFLLLRPPRK
                     DAVDPETGELMDDVLMFRTQGQEGKGRWLDDPALPLFTTPHFDGWPAVLMRVRHLDAA
                     DAASLRELVEHAWAAQAPKSLVKAYLAGRDAPP"
BASE COUNT          158 a          339 c          397 g          200 t
ORIGIN      
        1 ggaggctgta gcctgtctgg cgcccggtcc ccagccggcc tgcgcgcctg gtcaccgcga
       61 ttttcaacgc tcgctttgcg cggggcgggc agcttgcccg cagcgggctt gggtctacgg
      121 cggcgcgtcg cgtccggcca ggtaggcctt gacgagcgac ttcggcgcct gcgcggccca
      181 cgcgtgctcg acgagctcgc gcagcgacgc ggcatcggca gcgtccagat gccgcacgcg
      241 catgagtacg gcgggccagc cgtcgaagtg gggcgtcgtg aaaagaggga gggcggggtc
      301 gtccagccac cggcccttgc cttcctggcc ttgggtcctg aacatgagca cgtcgtccat
      361 cagttcgccc gtctccggat cgacggcgtc cttgcggggc ggcctcagga gcaggaagct
      421 cttgcccctg acgcggtact ccggtcgacc cgcggcggag aggtgggcat cggcctccgg
      481 catggcgcgc gctagccggt ctaggtcctc gaaggtgggc atggcgttac tgtagcgggc
      541 cgggggcggg gctcaccggc atcaccacag gaacgcgtga atgtctagga gcagcttcac
      601 ctgaagcggc gctcgagtcc ttccggcaga gggtcgaaga agctgtcggg cacgaccaac
      661 ccttccgctc catcgggagt gcgggtgcgc ctgggggcta gttcgtgtcg agcgttgccc
      721 ctttcgctct tggggtcggg gagcccgggt tcttcttgag aagtgggtac ttgtgcaact
      781 aggtgattgc gcaactgtct cgttgcatgg cgtctcgttt ttgtcctagg cctcccatgg
      841 tcgccgtgct ggcttgggcg gtagcgtctt ggaaggtagt cgtgtgtgac gcctgacgct
      901 gtgccgtcaa gagtgaacag accctagtcg cgggaccaac ggtctcgggc taggcgcggg
      961 gctcgttggg caagcgtgga gaagcaagtc cgcccagaga ctcgacgcga acgtaccgag
     1021 gaggcctcac gccgcggtcg aactctcaac tgaaggtggc cacctgagcc ggccaggttt
     1081 gcgataaccc ggcc
//
LOCUS       DAOQYE010000923         1094 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_181054_length_1094_cov_1.859480, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000923 DAOQYE010000000
VERSION     DAOQYE010000923.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1094
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_181054_length_1094_cov_1.859480"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            33..935
                     /locus_tag="PKN52_12280"
     CDS             33..935
                     /locus_tag="PKN52_12280"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNR00776.1"
                     /translation="MPAPAKPRPKREVVMAVVQPSANAVVRLLERTSVAPWQVVVGHT
                     LLGFLAAALVASASHANWLAAAVLLQVKTVLDNVDGGLARATGRVTEFGRYLDTLCDL
                     AVNVALFAALSRHGSALGSAAAFAIVTVALSADFGAQKRYEEVRGLRTRPATGAAPNG
                     RSSSASSSAEPGMSPGPTTRSRADSSATAPWLLKTARSVYRLVLAPQDRAYEALDSRL
                     FRTASGQAWGHAIGAERERWADLFSTGALVNLGLSTQYLALGVALALGMPYAYVVICW
                     CQLPYLLTVQAVRVLRYRGVGGRR"
     gene            971..>1094
                     /locus_tag="PKN52_12285"
     CDS             971..>1094
                     /locus_tag="PKN52_12285"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNR00777.1"
                     /translation="MRGRPAALVTGAAKGIGKAIALALAADGYDVMIHYRRSREE"
BASE COUNT          149 a          377 c          403 g          165 t
ORIGIN      
        1 gccgtccgag gcgcccctgt tagccttagc gcgtgccggc accagccaag ccccgcccga
       61 agcgggaggt cgtgatggcg gtcgtccagc cgtcggcgaa cgccgtcgta cggctgctcg
      121 agcgtacgag cgtcgccccg tggcaggtgg tggtcggtca cacgctcctg ggtttcctgg
      181 cggcggccct cgtggcgagc gcaagccatg ccaactggct cgccgcagcc gtgctgctac
      241 aggtgaagac cgtcctcgac aacgtcgacg gtggcttggc cagggcaacc gggcgggtca
      301 cggagttcgg ccgctacctc gacacgctct gcgacctcgc ggtcaacgta gcgctcttcg
      361 cggcgctctc ccggcatggg tcggcgctgg gaagcgcggc ggcgttcgcg atcgtgacgg
      421 tggccttgag cgccgacttc ggtgcccaga aacgttacga ggaggtgcgc gggctgcgta
      481 ctagacccgc gaccggggcc gcgccgaacg gcaggtcgag cagcgcgtcg agcagcgccg
      541 agccgggtat gagccctggg ccgaccaccc ggtcgagggc cgactcctcg gcaacggccc
      601 cttggcttct gaagacagcg cgaagcgtgt accggctcgt gttggcgccg caagacaggg
      661 cctacgaggc gctcgactcc cgcctgttcc gaacggcaag cggccaggcg tgggggcacg
      721 ctattggcgc cgagcgcgag cgctgggccg acctcttctc caccggcgcg ctcgtcaacc
      781 tcggcctcag cacgcagtac ctggcgctcg gggtggccct ggcgctcggc atgccctacg
      841 cttacgtcgt gatctgctgg tgccagctgc cttacctatt gaccgtccag gccgtcaggg
      901 tcctgcgcta ccgcggggtg gggggccgac ggtgagcggg tcgcgctccg gacctgtcgc
      961 acgcggccag gtgcgcggac gcccggccgc gctcgtcacc ggcgcggcca aggggatcgg
     1021 caaggccatc gcgttggcgt tggcggcgga cggttacgac gtgatgatcc actaccgcag
     1081 gagccgcgag gagg
//
LOCUS       DAOQYE010000924         1090 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_182029_length_1090_cov_3.493720, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000924 DAOQYE010000000
VERSION     DAOQYE010000924.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_182029_length_1090_cov_3.493720"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1090)
                     /locus_tag="PKN52_12290"
     CDS             complement(<1..>1090)
                     /locus_tag="PKN52_12290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00778.1"
                     /translation="TAQVNLTSPERQALEQDLARYQDLLAERQLQLVEIEKALGQTGG
                     QLEQRIAERDEVSEQLAAKRREREQLLSRIAGLEVERAATEAQVAALDDRLASMAERI
                     QDLLLSHYKQRGRDFTSGLATSRTFHELRVRNHYLGILAEQDADVVNELDAVLNERRA
                     AKAQLEAQLLELQAAEADLAKAETDLAATSQRLEQVLSELNATRAGQLILQQNLLEEQ
                     TRLEGSLGDVNRQLEQEIARLRAEEEAARAAAQQYAQDREKQLELQRQADEARSRADA
                     LTAPLAPLTTGFTRPFDGAKLISRFGEGNNSYLGIQAPVNNSAVRAVQTGRVSAVTYL
                     GANFGYMVGVQHAGGLTSVYVNLRQPLVD"
BASE COUNT          123 a          366 c          395 g          206 t
ORIGIN      
        1 gtcgacgagg ggctggcgga ggttgacgta gacgctcgtg aggccccccg cgtgctgaac
       61 gcccaccatg tagccgaagt tggcgcccag gtaggtaacg gctgagacgc ggcctgtttg
      121 cacggcgcgc acggccgagt tgttcaccgg cgcctggatg ccgaggtacg agttgttccc
      181 ttcgccgaag cggctgatga gcttcgcccc gtcgaacggg cgtgtgaagc cggtggtcag
      241 gggcgcgagc ggcgcggtca gggcgtcggc ccggctgcgc gcctcgtcgg cctggcgctg
      301 cagctcgagc tgcttctctc ggtcctgcgc atactgttgc gccgccgcgc gcgccgcctc
      361 ctcttccgcc cttaggcgcg cgatctcctg ctcgagttgg cggttgacgt cgccgaggct
      421 cccctcgagc cgcgtctgct cctcgaggag gttctgctgc aggatgagct gaccggcgcg
      481 cgtggcgttc agctcgctca ggacctgttc gaggcgctgg ctggtggccg cgaggtcggt
      541 ttcggccttg gccaggtcgg cctcggccgc ctgcagctcc aggagttgcg cctcgagctg
      601 cgccttggcg gccctgcgtt cgttgaggac cgcgtcgagc tcgttgacga cgtcagcgtc
      661 ctgctccgcc aagatgccaa ggtagtggtt gcgcacgcgc agctcgtgga aggtcctgct
      721 cgtggcgaga ccggacgtga agtcgcgccc gcgttgcttg tagtggctca gcagtaggtc
      781 ctggatgcgc tcggccatgg acgccagacg gtcgtccagg gcggcgacct gagcctcggt
      841 agcggcgcgc tccacttcca ggccggcgat gcgggacaac agctgttcgc gctcgcggcg
      901 cttggccgcg agctgctcgc tgacctcgtc acgctccgcg atgcgctgct cgagctggcc
      961 gcccgtctgc ccgagcgcct tctctatctc cacgagctgc agctgccgct cggcgagcag
     1021 gtcctggtac ctggcgaggt cctgctcgag cgcttggcgc tcggggctgg tgaggttcac
     1081 ctgggccgtc
//
LOCUS       DAOQYE010000925         1088 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_182704_length_1088_cov_2.241045, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000925 DAOQYE010000000
VERSION     DAOQYE010000925.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_182704_length_1088_cov_2.241045"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..616)
                     /locus_tag="PKN52_12295"
     CDS             complement(<1..616)
                     /locus_tag="PKN52_12295"
                     /inference="COORDINATES: protein motif:HMM:NF016240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HNR00779.1"
                     /translation="MALTDIELRVLGALVEKERTTPEAYPLSSQALHTACNQKTSRDP
                     VTDYHLQDVLAALQRLHDKGLTATVQEVSDRVPKHRHKLAQAWSVDAKELAVLSVLML
                     RGPQTPGELRTRTERYTTFPDLASVERVLAGLAERRVPLVENLGRGPGQSQDRWAHKL
                     GLDEERMQPRVRSRQPDEAREGNGHEGHHANGSALAVLLRRVEDL"
     gene            693..>1088
                     /locus_tag="PKN52_12300"
     CDS             693..>1088
                     /locus_tag="PKN52_12300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00780.1"
                     /translation="MPIRDELIERTMQQLAALMAKILHARADVVGAAQFDIGSAESKL
                     DELYLANLGTSRSLVRRLGLEDLLGVIGTAGHVDGERAYVLGALLSTEAEVVIQAGAS
                     EEDLEVLGLRNAALALFLEAGAERLGEPDL"
BASE COUNT          157 a          376 c          382 g          173 t
ORIGIN      
        1 ccaggtcctc cacgcgcctc aacaacacgg cgagggcgga gccgttcgca tggtggccct
       61 catggccgtt cccctcgcgt gcctcgtccg gctggcgcga gcgcacgcgc ggctgcatgc
      121 gctcctcgtc gagccccagc ttgtgcgccc aacggtcctg gctctggccg gggccgcggc
      181 cgaggttctc gacgagcggc acgcgccgct cggccaggcc ggcgagcacc cgctcgacgc
      241 tggccaggtc cgggaacgtc gtgtaccgct ccgtgcgcgt gcgcaactcg cctggcgtct
      301 ggggtccgcg gagcatgagg acgctcagca cggccaactc cttggcgtca accgaccacg
      361 cctgtgcaag cttgtgacgg tgcttcggca cgcggtcgct gacttcctgc accgtcgcgg
      421 tgaggccctt gtcgtgcaag cgttgcaagg cggccaggac gtcctgcagg tggtagtcgg
      481 tgacggggtc gcggctggtc ttctggttgc aggcggtgtg tagagcttgg ctgctaagcg
      541 gatacgcttc gggcgtggtg cgctccttct ccacaagagc gccgagcacg cgcagctcga
      601 tgtctgtcag agccatgcgc gcaggatatt acggctcgaa ccagtgacca gtgggctgcg
      661 ccccagccgc ccgggtgtaa taccgtaggc gcgtgcccat cagagatgaa ctcatcgagc
      721 gcaccatgca gcaactcgcc gcgctcatgg ccaagatcct ccacgcacgc gccgacgtgg
      781 tcggcgcggc gcagttcgac atcggctcgg cggagagcaa gctcgacgag ctttacctcg
      841 cgaacctcgg gacgagccgc agcctcgtgc gacggctggg gctcgaggac ctccttgggg
      901 tcatcggcac cgccggccac gtcgacggcg aacgcgccta cgtgctcggc gctctgctca
      961 gcacggaggc cgaggtcgtg atccaggcgg gcgcaagcga ggaggacctc gaggtcctcg
     1021 gcctgcgcaa cgcggcgctg gcgctgttcc tcgaggcggg agccgagcgc ctaggcgagc
     1081 cggacctc
//
LOCUS       DAOQYE010000926         1087 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_182926_length_1087_cov_3.064922, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000926 DAOQYE010000000
VERSION     DAOQYE010000926.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_182926_length_1087_cov_3.064922"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..595)
                     /locus_tag="PKN52_12305"
     CDS             complement(<1..595)
                     /locus_tag="PKN52_12305"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNR00781.1"
                     /translation="MFTPADREKLRNELIETARADADVVGAAIVGSVAAGREDAWSDI
                     DLALQVRGDADPPAVADRWTAAFYASHGAAHHVDVFARGVLYRVFLLASSLQVDVSFW
                     PEDRFQATGPAFSVIFGSPRPPAEPARHDAAQTVGMAWLYALHSRSAVVRGRTWQAVM
                     MLDHLRDQVLTLACLRLGFNAYDGREANLLPRSLLDAH"
BASE COUNT          184 a          387 c          340 g          176 t
ORIGIN      
        1 catgggcatc caacagcgaa cggggcagga gattggcctc ccggccgtcg taggcgttga
       61 aaccgagtct caggcaggcg agcgtcagga cctggtccct aaggtggtcg agcatcatga
      121 ccgcctgcca cgtgcggccg cgcacgacgg cggagcggga gtgcagcgcg tacagccagg
      181 ccatcccgac ggtctgcgcg gcgtcgtggc gggccggctc cgcgggcggg cgaggtgacc
      241 cgaagatgac gctgaaggcc gggcccgtgg cctggaaccg gtcctccggc cagaaggaga
      301 cgtccacctg caaggacgag gcgagcagga acacccggta gagcacccct ctggcgaaga
      361 cgtcgacgtg gtgggcggcg ccgtgcgagg cgtagaacgc ggccgtccaa cggtcggcca
      421 cagccggcgg gtcggcgtcg cccctcacct gcagggccag gtcgatgtcg gaccaagcgt
      481 cctcgcggcc agcggccacc gagccgacga tggcggcgcc gaccacgtcc gcgtcggctc
      541 gtgcggtctc gatgagctcg ttgcggagct tctcgcggtc ggcgggggtg aacatcgcta
      601 ggaccttgaa ccccgcttac catgagtcag ccacaagtcc aactccactc tgtcgtgagc
      661 gacccggggc acgagtcggc ggccttgacc cttgtccata cgcacaagcc catagaccgc
      721 catcttcctc agcgtgcgag acaagttgga ttttgcgcgc cccgtcatct ctgccaactc
      781 cccaaccgag cccggggcct tctcgtctat gactctcagc agttccctat ttcccgccga
      841 caggatccgc gcgaatgact ctatggacgt gaaccatacc ttcggtgtct cttcggtgac
      901 cttctcttca ccgcgcgcaa cccgcatggt ccaagccttc atctctccgt gatcggcaat
      961 ccctactcgc aacgtcgtca tcgccttacc tcgttctcgc aacaccgcgt cgacctctcg
     1021 ccagagctcc cgtatcgagg ccggtcgcct cgcccgaagt ggcgatcacc cgccgcgcca
     1081 cgcgcac
//
LOCUS       DAOQYE010000927         1085 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_183470_length_1085_cov_3.876699, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000927 DAOQYE010000000
VERSION     DAOQYE010000927.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_183470_length_1085_cov_3.876699"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..830
                     /locus_tag="PKN52_12310"
     CDS             <1..830
                     /locus_tag="PKN52_12310"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNR00782.1"
                     /translation="RFFTRLWRNRVGFIGFVVALVVVLAAALGPLIAPYDPLAQSIRE
                     RFQGPSGTHLLGTDNFGRDTLSRILYGYRTSVLVSLGAVLIATVVGGGLGLLAAYWGG
                     WRDRLIMRVMDVLLAFPIILLAIAVLAVLGPGSLNTGLAIGIVYTPVFARLARGPALT
                     VLSWDYVAAARALGGGAVRVLSKHVAPNIMAPILVQVTLSLSTAILVEASLSFLGLGT
                     QPPTPSLGLMLSESRGTMLLSPWVSVFSGLAILLASFGFNLFGDGLRDLLDPTLRDV"
BASE COUNT          132 a          435 c          324 g          194 t
ORIGIN      
        1 agcgtttctt cacgcgcctg tggcgcaacc gcgtggggtt catcggcttc gtggtcgccc
       61 tcgtcgtggt cctggcggcc gccctcggac cgctgatcgc gccgtacgac cccctcgccc
      121 agtccatccg tgagcggttc cagggccctt ccgggaccca cctgctcggc acggacaact
      181 tcggccgcga cacgctctcg cgcatcctct acggctaccg caccagcgtg ctcgtcagcc
      241 tgggcgcggt gctcatcgcc accgtcgtcg ggggcgggct cggcctcctt gccgcctact
      301 ggggcggctg gcgcgacagg ctgatcatgc gcgtcatgga cgtgctcctc gccttcccca
      361 tcatcctgtt ggccatcgcg gtgctcgccg tcctcggccc gggcagcctc aacacgggcc
      421 tggccatcgg catcgtctac acacccgtct tcgcgcgcct cgcgcgcgga ccggccctca
      481 ccgtgctgag ctgggactac gtcgccgcgg cgcgcgcgct cggcggcggc gcggtgcggg
      541 tgctctccaa gcacgtcgcg ccgaacatca tggcccccat cctcgtgcag gtgacgctct
      601 cgctctccac ggccatcctg gtggaagcgt ccttgagctt cctcggtctc ggcacacagc
      661 cccctacccc gtcgctaggg ctcatgctct ccgagagccg cggcaccatg ctcctctcgc
      721 cgtgggtatc ggtgttctcc ggcctcgcca tcctactcgc ctcgttcggg ttcaacctgt
      781 tcggcgacgg cctgcgcgac ctgctcgacc ccaccctgcg cgacgtctga acacacagcc
      841 gtcgccatac cggcaacgcg cttcgcccct gtcgctccga gccgtcggca ggcgtcttaa
      901 gcgttgctca gggtggcgca ggccgtccct cacacgccca aggccacgtt gcccatacac
      961 tcgaggcatg accaggacca accgacgccc ggccgcgtta gtcgcctcgc tcctactcgc
     1021 caccttgctg gcgacggcct ccgcacaggt ggacgcacgc gcacgagccc tactagaggg
     1081 gctgc
//
LOCUS       DAOQYE010000928         1084 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_183925_length_1084_cov_1.897959, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000928 DAOQYE010000000
VERSION     DAOQYE010000928.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1084
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_183925_length_1084_cov_1.897959"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(252..680)
                     /locus_tag="PKN52_12315"
     CDS             complement(252..680)
                     /locus_tag="PKN52_12315"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HNR00783.1"
                     /translation="MQEALVRERLKLLASYIDDLQGVRETSEAEYAGNIVLQRFVERT
                     LHLAIEACHYVGFHVISSEGYREPRLAREVVDVLADNGWIGRDTRERLQGMAGFRNLL
                     VHDYATIEPRLVLEVLRTRLEDLSAFGAEVPHRLDAAAAP"
     gene            complement(833..>1084)
                     /locus_tag="PKN52_12320"
     CDS             complement(833..>1084)
                     /locus_tag="PKN52_12320"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNR00784.1"
                     /translation="TRLASELAGESDVVAAYLFGSTARGQAQGVGSDVDIGVLFTDTP
                     PTVERLFRRVEIARLLEAALEVAVDVVDLRAAPPTSTIR"
BASE COUNT          168 a          369 c          379 g          168 t
ORIGIN      
        1 gtcgttgatg accgcgtaga cgcgcacctc gccctgcgcc gcggcgcgac ccggcaggag
       61 caacccgagg agcgcgaggc cgacgaggcc tgcgcggaag cgggccgcac gggaacggtt
      121 gagtggctga cgcatcaggc gatctccttg acgaggctcc cgcgcgctgc cggtgagccc
      181 aacagcagtc taggccccgc gaggcagcgc ggggtcgacg ggggcacggg gcgggtgcgc
      241 caggggcggg ctcagggcgc ggcggcggcg tcgagcctgt ggggtacctc cgcaccgaac
      301 gcgctcaggt cctcgagcct ggtcctcagc acctcgagca ccaaccgggg ctctatcgtc
      361 gcgtagtcgt gcacgaggag gttcctgaac cccgccatcc cctgaagccg ttccctggtg
      421 tcccgaccta tccagccgtt gtcggccagc acgtcgacga cttcccgggc gagacgaggt
      481 tcgcggtagc cctcgctgga gatcacgtgg aagccgacgt agtggcaggc ctcgatggcg
      541 agatgcaggg tgcgctccac gaagcgctgc agcacgatgt tgccggcgta ctccgcctcg
      601 ctagtctcgc gcacgccctg caagtcgtct atgtagctcg cgagcagctt caagcgttcc
      661 cggacgagtg cttcctgcac cacgacggcc ccctccttcc ctgagcctgc gcacgctctg
      721 ctcgtcgtga cggcggcggt aggggagcag gtcgaagtag tcgcgcctca ctccctgctc
      781 gaacgcgatg cgagcgggcg acgtcccccc ttgagtagga cgccgtcggt cattacctga
      841 tggttgaggt ggggggcgcg gcgcggaggt cgaccacgtc gacggctacc tccaacgcgg
      901 cttcgagtag cctcgcgatc tcgacgcggc ggaacaggcg ttccaccgtg ggaggcgtgt
      961 cggtgaacag cacgccgatg tccacgtcgc taccaacacc ctgcgcttgt ccacgagcgg
     1021 tggagccgaa caggtaagcg gccacgacgt cgctttcgcc agcgagttcg ctcgcgagcc
     1081 tggt
//
LOCUS       DAOQYE010000929         1084 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_183950_length_1084_cov_1.732750, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000929 DAOQYE010000000
VERSION     DAOQYE010000929.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1084
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_183950_length_1084_cov_1.732750"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            107..>1084
                     /locus_tag="PKN52_12325"
     CDS             107..>1084
                     /locus_tag="PKN52_12325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00785.1"
                     /translation="MEDEGDVDYVDENILAAIDKQIAANKRLDETTDEYTATLDAYSD
                     LLSLCETSEQLEDTFLAIKHHPGWAELDSEGQTEVTQNFISYMDSIARGDPRYSRASG
                     PKAREPMASEVFRELIENANRTLPAGSPQVVGVDTVAEFEAATGHAAPSDARGVFVDG
                     SIYMVRENLGDPKDFVFVLAHERGHHGLDTLLGDRLPAVVNRLWTNAATRDRIKARMK
                     LLNESPEVGSLRRAAAEEVLMDMLAGGERVNGDVLAKVRSAIDNAFAHLLGVSHLRMT
                     NAEVDALLRDVALAANGRTPKGSGKAPHLQGLEFALEDPAQFVAGDPKFS"
BASE COUNT          209 a          357 c          364 g          154 t
ORIGIN      
        1 tgccgtcgca gactcccccc gtcgagccga agggcaggcg ctgcccgagg tgcttagcag
       61 gtcgggccag caacctgcag cgccgacgca acccgcgtca gagcaggtgg aggacgaggg
      121 cgacgtcgac tatgtcgacg agaacatcct tgcggccatc gacaagcaga tcgcggccaa
      181 caagcggttg gacgaaacta ccgacgagta caccgccacg ctcgacgcgt actccgacct
      241 gctcagcctg tgcgagacct ccgagcaact ggaagacacg ttcttggcga tcaagcacca
      301 cccgggttgg gccgagctgg actccgaagg ccagacagaa gtgacccaga acttcatctc
      361 gtacatggac tccatcgcac gcggtgaccc acgctattcg cgggcgagcg gccccaaggc
      421 gagggagccg atggccagcg aggtgttccg cgaactgatc gagaacgcca accgcaccct
      481 cccggcgggc agcccgcagg tggtgggggt cgatacggtg gcggagttcg aggcagccac
      541 tgggcacgcg gctccgagcg acgcacgcgg cgtgttcgtc gacggcagca tctacatggt
      601 tcgggaaaac ctcggcgacc ccaaggactt cgtctttgtg ttggcgcatg aacgcgggca
      661 ccacggcctc gacacgttgc tgggcgaccg actgccggcg gtggtgaacc gcctgtggac
      721 caacgcggcg acacgcgacc ggatcaaggc ccggatgaag ctgctcaacg agagtcccga
      781 ggtaggctcc ctgcgtcgcg ccgcagcgga ggaggtgctg atggacatgc tggccggcgg
      841 cgagcgcgta aacggcgacg ttctcgccaa ggtccggtcg gctatcgaca acgcgttcgc
      901 acacctgctc ggcgtctccc acctgcggat gaccaacgcc gaggtggacg ccctcctgcg
      961 cgacgtggcc ctcgctgcca acggtcgtac gccgaagggg agtggaaagg cgcctcacct
     1021 gcaagggttg gagttcgctc tggaagaccc agcgcagttc gtcgcgggcg accccaagtt
     1081 ctcc
//
LOCUS       DAOQYE010000930         1082 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_184367_length_1082_cov_2.866602, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000930 DAOQYE010000000
VERSION     DAOQYE010000930.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_184367_length_1082_cov_2.866602"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="PKN52_12330"
     CDS             <1..442
                     /locus_tag="PKN52_12330"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNR00786.1"
                     /translation="FYPAGLIMDRLGRKFAIVPSFALQAVGMALVPLTTEATGLALAA
                     LLMGFGNGISAGTMLTLGADLAPRNAVATFLGWWRLIGDAGFVAGPLITGAVASGLGL
                     GSSALVVGALGAGAAVWFAFGVPETLKRPQVEPASLSPGQLQDP"
     gene            complement(410..>1082)
                     /locus_tag="PKN52_12335"
     CDS             complement(410..>1082)
                     /locus_tag="PKN52_12335"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HNR00787.1"
                     /translation="IMHFEGAEPIGPDLCALDTYHAAGLRSLGPVWSRPNLFGFGVPF
                     EFPHSPDMGPGLTPHGLALVRRCNELKIMLDLSHITEKGFWDVARTSDAPLVATHSNA
                     HAICPSPRNLTDEQLAAVRASNGLVGLNYNTGFLSPDGSYDTAMPLSVMVRHVDYLVD
                     KLGVDGVALGSDFDGATMPEGVKDAAHLPNLMNALAEAGYDDEALRKIGYRNWLRVLK
                     LTWGE"
BASE COUNT          139 a          376 c          388 g          179 t
ORIGIN      
        1 cttctacccg gcgggcctga tcatggaccg gctgggccgc aagttcgcca tcgtcccctc
       61 gttcgccctc caggcggtcg gcatggcgct cgtgcctctc acgaccgaag ccaccggcct
      121 cgcgctcgcc gccctgctca tgggcttcgg caacggcatc agcgcaggga ccatgctgac
      181 gctcggcgcc gacctcgccc cgaggaacgc cgtggcgacg ttcctaggct ggtggcgcct
      241 catcggcgac gccggcttcg tggccgggcc gctcatcacg ggcgctgttg ccagcggcct
      301 cgggctcggc tcgtcggccc tggtggtcgg ggcgctcggc gccggcgcgg ccgtctggtt
      361 cgcgttcggg gttcccgaga cgctcaagcg gccgcaggtc gagccggctt cactctcccc
      421 aggtcagctt caagaccctt agccagttcc ggtagccgat cttgcgcagc gcctcgtcgt
      481 cgtagccggc ctccgccagg gcgttcatga ggttgggcag gtgcgcggcg tccttgacgc
      541 cctccggcat cgtcgcgccg tcgaagtcgg agccgagcgc cacgccgtcg acgccgagct
      601 tgtcgacgag gtagtcgacg tgcctgacca tcacggacaa cggcatggcc gtgtcatagc
      661 tgccgtcggg gctcaggaag cccgtgttgt agttcagccc caccaggccg ttcgaggcgc
      721 gcacggccgc caactgctcg tctgtcaggt tgcgcggcga cgggcagatg gcgtgggcgt
      781 tcgagtgggt ggccacgagg ggcgcgtcgc tcgtgcgcgc cacgtcccag aagcccttct
      841 ccgtgatgtg cgagaggtcg agcatgatct tgagctcgtt gcagcgcctg acgagcgcga
      901 ggccgtgggg tgtcaggccc gggcccatgt cggggctgtg cgggaactcg aacggcacac
      961 cgaacccgaa gaggttcggc cggctccaca cgggcccgag cgagcgcagg ccggcggcat
     1021 ggtacgtgtc gagcgcgcac aggtcggggc cgatgggctc ggccccctcg aagtgcatga
     1081 tg
//
LOCUS       DAOQYE010000931         1079 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_185293_length_1079_cov_3.100586, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000931 DAOQYE010000000
VERSION     DAOQYE010000931.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1079
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_185293_length_1079_cov_3.100586"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            160..1056
                     /locus_tag="PKN52_12340"
     CDS             160..1056
                     /locus_tag="PKN52_12340"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HNR00788.1"
                     /translation="MTSERARSSRVTGVAREGPGERSGVHDQGAPGRAHPAEHEGRSR
                     NETPSIGAYREEPAWNGATSPWRAAAPWASGLPDTVQGLLAGLAPCPVCTVRPAGGAG
                     VCGPCRARLRAALAPPGEASRARPGDVVWLGPYAGTWLRLVHALKYRGARRLADFLGE
                     LLAMRIEASGWCSGPEALDLVTHVPTTTRRKAERGYDQAELLASAVADRLGLRRLAVL
                     RRVHATERLAGRGRAERAALLAGAFRARYLPGRNVLLVDDVLTTGATLGSATEALLRQ
                     GASSVRCVVVARTYRAGAVTPT"
BASE COUNT          132 a          398 c          404 g          145 t
ORIGIN      
        1 tgggaaacct ggctgccagg gcggcatgaa gggcgtgttc tgagcttgcg gtgacgggcg
       61 acacgttgtc ggccggcgct ccgggcgcgc accgccttca actaggctcc ggcctaccgt
      121 gggtcacgcc ggccggtgac acgccgctat agtcgcgcca tgacctcgga acgcgcccgc
      181 tcgtcacgcg tcacgggggt cgctcgtgag gggccgggtg agcgctcggg tgtccacgac
      241 cagggcgcgc caggcagggc tcacccggcc gagcacgagg ggaggtcccg gaacgagaca
      301 ccctccatcg gcgcctatcg ggaagagccc gcctggaacg gtgccacctc gccatggcgc
      361 gcggcggcgc cgtgggcttc gggcctgccg gacaccgtcc aaggcctgct cgcgggcctt
      421 gcaccctgtc cggtctgcac cgtcagaccg gccggcggcg ccggcgtctg cggtccgtgt
      481 cgagcgcggt tgcgcgcggc cctcgcgccg ccgggcgagg cgtcgcgcgc gaggccgggc
      541 gacgtcgtct ggctcgggcc ttacgccggc acgtggctca ggctcgtcca cgccctgaag
      601 taccgaggcg cgcggcggct ggccgacttc ctgggcgagt tgctcgccat gcggatcgag
      661 gcgagtggct ggtgctccgg cccggaggcg ctcgacctcg tgactcacgt ccccacgacc
      721 acgcgacgca aggccgagcg cggctatgac caggccgagc tcctcgcgtc cgccgtggcg
      781 gaccgcctag gcctgcgccg gctcgccgtc ctacgccgcg tccacgccac ggagagactc
      841 gccggccgag gccgggccga gcgagccgcg cttctcgccg gcgccttccg ggcccggtac
      901 ctcccgggcc gcaacgtgct gctcgtcgac gacgtgctca cgaccggagc cacgctcggc
      961 tcggccaccg aggcgctgct gcgtcaaggt gcgagcagcg tgcgctgcgt cgtggtggcc
     1021 cggacctacc gggcgggcgc ggtaacgcct acatgacgac gacgatggcg gtcaggatg
//
LOCUS       DAOQYE010000932         1079 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_185453_length_1079_cov_1.744141, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000932 DAOQYE010000000
VERSION     DAOQYE010000932.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1079
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_185453_length_1079_cov_1.744141"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="PKN52_12345"
     CDS             <1..516
                     /locus_tag="PKN52_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HNR00789.1"
                     /translation="LKNLVDTMRQTTQLTSMVFIILVGATAFSMVFTALRGDTLVDQF
                     LVNLPGGKWGFLFFTMLTIFVLGFFIDFIEITFIVVPFITPIAAHFFGQDMLLWFGVV
                     IAMNLQSSFLTPPFGFALFYLKGVAPPQIKTEHIYRGIIPFVLIQIVALLLLVAFPGI
                     VQWLPSISNAQ"
     gene            747..>1079
                     /locus_tag="PKN52_12350"
     CDS             747..>1079
                     /locus_tag="PKN52_12350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00790.1"
                     /translation="MIPRTRHIESSTTSVVAPNLLERVGVRPPYLALTDIDMAGLGLR
                     ATAAAESPSYSELGPMPAAELGRHAAIVGLLTAAARQGDDRRRYYLARRAECRYVPND
                     APYGAPVRF"
BASE COUNT          156 a          366 c          345 g          212 t
ORIGIN      
        1 ctcaagaacc tggtcgacac catgcgccag accacccagc tcaccagcat ggtgttcatc
       61 atcctggtgg gcgccaccgc cttcagcatg gtgttcacgg ccctgcgcgg cgacaccctc
      121 gtcgatcagt tcctcgtcaa cctgccgggc gggaagtggg gcttcctgtt cttcaccatg
      181 ctcaccatct tcgtgctcgg cttcttcatc gacttcatcg agatcacctt catcgtcgtg
      241 ccgttcatca cgcccatcgc ggcacacttc ttcgggcagg acatgctgct ctggttcggc
      301 gtcgtcatcg ccatgaacct gcagagctcg ttcctcaccc cgccgttcgg cttcgcgctc
      361 ttctacctga agggggtggc gccgccgcag atcaagacgg agcacatcta ccgcggcatc
      421 atcccgttcg tgctcatcca gatcgtggcg ctactgctgc tcgtggcttt ccccgggatc
      481 gtgcagtggt tgccttcgat ctctaacgcg cagtaggccg ggccgtactc accccgggtt
      541 cgccgggggc gacgcggatg accgggtcgg gtgaggtcgc cagcgcgagg cggcggacct
      601 cggctagccg cccgcatcgt ccaggaggtg gtagcttctc ccgtgggggg cgtaggggag
      661 aggtctggga tgatcgacaa ccgttcgtgg gggcagttgg ttgcttgccg acagcaggat
      721 ctggagcgcg cgttggggac cgcacgatga tcccgcgcac gcgacatatc gagagctcga
      781 cgacgagcgt tgtcgccccg aacctgctgg agcgggttgg ggtcaggccg ccttatctcg
      841 cactgactga catcgacatg gcgggccttg ggctgcgggc gaccgccgcg gccgaatcgc
      901 cgagctactc ggagctcggc cccatgcccg cggccgagct tggcagacac gccgccatcg
      961 tcggcctgtt gaccgcggcg gctcggcagg gtgacgatcg ccgccgttac tacttggcgc
     1021 gccgcgccga gtgtcgttac gtacccaacg acgcgcccta cggtgcgccg gttcgcttc
//
LOCUS       DAOQYE010000933         1076 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_186253_length_1076_cov_2.261508, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000933 DAOQYE010000000
VERSION     DAOQYE010000933.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_186253_length_1076_cov_2.261508"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1076
                     /gene="pgi"
                     /locus_tag="PKN52_12355"
     CDS             <1..>1076
                     /gene="pgi"
                     /locus_tag="PKN52_12355"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HNR00791.1"
                     /translation="RVDGDDVMPGVNAVLAQMERFTDAVRSGAWRGHTGERITDVVNI
                     GIGGSHLGPQMVVTALQPYTTPALRAHFVSNVDGADIAGALAGLRPESTLFIVSSKTF
                     TTQETMTNAHTARRWLIERLGEEAAVARHFVAVSTNLEQVAAFGIDEANAFAFWDWVG
                     GRYSLWSAIGLSIALAVGFQRFVELLEGAHELDRHFLNAPPERNVPLLHGLLAVWYGN
                     FLGAESWAVLPYDESLRYLPAYLQQAYMESNGKDVDRAGKSVTWQTGQVVWGAPGTNG
                     QHAFYQLLHQGTKLIPADFIVPARPLHPLIDHHDILLSNFLAQTEALMLGRTVDETRR
                     ALSGHGLAPERLEHFLGLEPAQHH"
BASE COUNT          169 a          397 c          339 g          171 t
ORIGIN      
        1 gcgcgtcgac ggcgacgacg tgatgccggg agtgaacgcc gtgctcgcgc agatggagcg
       61 cttcacggac gccgtgcgct ccggcgcttg gcgcggccac acgggcgaac gcatcacgga
      121 tgtggtcaac atcggcatcg gcggctcgca cctgggtccg cagatggtcg tcacggccct
      181 gcaaccgtac acgacgcccg cgctccgcgc tcacttcgtc tccaacgtgg acggcgccga
      241 catcgctgga gcgctggccg gcctgcgacc ggagtcgacc ctcttcatcg tgtcgtccaa
      301 gacgttcact acccaggaga cgatgacgaa cgcccacact gcccgccgct ggctcatcga
      361 acggctgggc gaggaggcgg ccgtggcgcg gcacttcgtg gccgtatcga ccaaccttga
      421 gcaggtagcc gccttcggca tcgacgaggc caacgcgttc gcgttctggg actgggtcgg
      481 cggacgctat tcgctctggt cggccatcgg gctctccata gccctggccg tcggcttcca
      541 gcgcttcgtg gagctgctgg aaggcgcgca cgagctcgac cgccacttcc ttaacgcccc
      601 gccagagcgc aacgtcccgc tgctccacgg cctgctcgcc gtgtggtacg gcaacttcct
      661 cggcgccgag agctgggcgg tcctgccgta cgacgagtcg ctgcgctacc tccccgccta
      721 cttgcagcag gcgtacatgg agagcaacgg caaggacgtc gaccgcgccg gcaagagcgt
      781 gacgtggcag acgggccagg tcgtatgggg cgccccgggc accaacggcc agcatgcgtt
      841 ctaccagctc cttcatcagg gcacgaagct gatccctgcc gacttcatcg tgccggctag
      901 gccgctccac cccttgatcg accaccacga catcctcctt tccaacttcc tggcgcagac
      961 ggaggcgctc atgctcggtc gcaccgtcga cgagacccgc cgcgcgctct ccggccatgg
     1021 cctggcgccc gaacgcctcg agcacttcct ggggctcgaa cccgctcagc accacc
//
LOCUS       DAOQYE010000934         1076 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_186414_length_1076_cov_1.216454, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000934 DAOQYE010000000
VERSION     DAOQYE010000934.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_186414_length_1076_cov_1.216454"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="PKN52_12360"
     CDS             complement(<1..229)
                     /locus_tag="PKN52_12360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HNR00792.1"
                     /translation="MALRVRITPEGYERLKATLEQEYARLAEATTILQELTGSSDDYD
                     DSGLEEAKAEKARIERRIDELELQLNRADVIE"
     gene            426..>1076
                     /locus_tag="PKN52_12365"
     CDS             426..>1076
                     /locus_tag="PKN52_12365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00793.1"
                     /translation="MRFRLVLLALLTLVAGTVQRPAAAASPTLHPVPAPYAELSAARA
                     TGDAATLERLAFADDSYAAYAAASELAAWPQLPPRLRLGALERALELRIPDALMRAET
                     VRLQLLRAELAEAVGDTAKATSAYRSAMPDPAAVAGFVRLETDPYRRAAGLLDANRYE
                     AALEALGGLAAPSIEAPALRALGRYGEALGAYRAWLAADPGNGAAALGAAWCHYYLR"
BASE COUNT          116 a          439 c          357 g          164 t
ORIGIN      
        1 cctcgatgac gtccgcgcgg ttgagctgca gctcgagctc gtcgatgcgc cgctcgatgc
       61 gcgccttctc cgccttggcc tcttccaggc cgctgtcgtc gtagtcgtcc gacgaccccg
      121 ttagctcctg gaggatggtg gtggcctccg ccaggcgggc gtactcctgt tccagggtgg
      181 ccttgaggcg ctcgtacccc tcaggcgtga tgcgaactct cagcgccatt ttccgtactc
      241 cttctctgtt tgctgcgcgt gcccgtggag ccgctagcaa gcggcttgtc ccttgcagga
      301 caagccgaaa ggccgctcga ctgtggcagg agtctaccgc accgggcggg gtggcggcgt
      361 cacctggccg accccgcgcc acccgccgct cacccagccg ccaccagccg tgcttacact
      421 tgggtatgcg gttccggctc gtcctgctcg cccttctaac gctcgtggcg ggcacggtgc
      481 agcgacccgc cgctgccgcc tccccgacgc tccacccggt gcccgccccg tacgcggaac
      541 tcagcgccgc gcgcgccacc ggcgacgccg ccacgctcga gcggttggcg ttcgccgacg
      601 actcctacgc ggcgtacgcc gccgccagcg agctcgccgc ctggccgcag ctcccgccac
      661 ggctccgcct cggggcgctg gagcgcgccc tcgagctgcg catccccgac gcgctcatgc
      721 gcgccgagac ggtgcggctt cagttgctgc gcgccgagct cgccgaggcg gtgggagaca
      781 ccgccaaggc gacctccgcc taccgcagcg ccatgcccga cccggccgcc gtggcgggct
      841 tcgtccggct cgagaccgac ccgtaccgcc gcgccgcggg cctgctggac gccaaccgct
      901 acgaggcggc cctcgaggcg ctcgggggcc tcgccgcccc ctccatcgag gcgcccgcgc
      961 tgcgggcgct gggccgctac ggcgaggccc tcggcgccta ccgcgcctgg ctcgcggccg
     1021 acccgggcaa cggcgcggcc gccctcggcg ccgcctggtg tcattactac ctgcgg
//
LOCUS       DAOQYE010000935         1073 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_187019_length_1073_cov_3.199411, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000935 DAOQYE010000000
VERSION     DAOQYE010000935.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_187019_length_1073_cov_3.199411"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..>1073)
                     /locus_tag="PKN52_12370"
     CDS             complement(2..>1073)
                     /locus_tag="PKN52_12370"
                     /inference="COORDINATES: protein motif:HMM:NF036552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5309 family protein"
                     /protein_id="HNR00794.1"
                     /translation="MSVNNFKFEWETANAPTRKFVCTNSSSGVEDSSGSNTALALTGG
                     AVIEKGTILRNCSRATPIGTYGADELLEVTANTAGALTVVRDVGRQNSGTGSTAHTLG
                     DYFEIVWAPKEEGSSVDRNKYTDVTLVSNYTNIVDFALNVTGSQAETKRLVGGDTLEA
                     QFQNCLRQLQNELEGMLLYGCLNNGANAGSDSYVRRTKGIDSFICASGANIDYSTKSV
                     TEEALNAQIAAILEDATDPTDRFIIVCHPQNARVISSFGSDKVITTPEKTKWGRYIDT
                     FRSDLGVDMDVIWTLNCSKSDLFILDMNKVAFAVFRPFAKATWTYGDDGVDAWRQRYL
                     GEVGVKVVDALYSHAKLGYISW"
BASE COUNT          188 a          306 c          364 g          215 t
ORIGIN      
        1 gttaccagga gatgtaaccg agcttggcgt gcgaatagag cgcgtcgaca acctttacgc
       61 cgacctcgcc gaggtagcgc tgacgccatg cgtcaacccc gtcgtcgccg taggtccacg
      121 tcgccttggc gaagggacgg aagacggcga aggcgacctt gttcatgtcg aggatgaaga
      181 ggtcgctctt ggagcagttc agcgtccaga tgacatccat gtcgacgccg aggtccgaac
      241 ggaacgtgtc gatgtagcgg ccccacttgg tcttctcggg ggtcgtgatg accttgtccg
      301 aaccgaagga gctgatgacc cgagcgttct gcggatggca gacgatgatg aagcggtcag
      361 tcgggtcggt tgcgtcctca aggatggccg cgatctgcgc gttcagagcc tcttcggtga
      421 cactcttggt ggagtagtcg atgttcgcgc cgctggcgca gatgaacgag tcgataccct
      481 tggtgcggcg cacgtaggag tccgacccgg cgttcgcgcc gttgttcagg cagccgtaga
      541 ggagcatccc ctccagttcg ttctggagct gccgcaggca gttctggaac tgagcctcaa
      601 gggtgtcgcc gccaaccaga cgcttcgtct ccgcctgaga gccggtgacg ttcagggcga
      661 agtcgacgat gttggtgtag ttggagacga gggtgacatc ggtgtacttg ttgcggtcca
      721 ccgaggagcc ctcttccttg ggagcccaga cgatctcgaa gtagtccccg agggtgtgcg
      781 ccgtcgagcc ggtgccactg ttctgacgac cgacatcgcg gacgacggtc aaggccccgg
      841 ccgtgttggc cgtgacctcc agaagctcgt cggccccata agtgccgatg ggggtcgcgc
      901 gggagcagtt gcggaggatg gtgcctttct cgatcaccgc gccgcccgtg agggccaagg
      961 cggtgttaga accgcttgag tcctcgacgc cgctagagga attggtgcag acgaacttgc
     1021 gggtcggggc gttcgccgtc tcccactcga acttgaagtt gttgacgctc atc
//
LOCUS       DAOQYE010000936         1070 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_187933_length_1070_cov_3.809852, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000936 DAOQYE010000000
VERSION     DAOQYE010000936.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_187933_length_1070_cov_3.809852"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..649)
                     /locus_tag="PKN52_12375"
     CDS             complement(<1..649)
                     /locus_tag="PKN52_12375"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HNR00795.1"
                     /translation="MEDVLAASDEQLARKPVHRGMITTGQVIMLAQEGIVVDVGAKIE
                     GMLPYNQLFEHESNAEEAAKYFKPGDDIQVYVIRSDIPNSTITLSKKRADQERAWNLL
                     QDIHENGKPVEVEIVEKVRGGLVASLGVRAFLPASQVDIRRVNDLAPYVGRRMKVKII
                     ELNRRRNRVIISRRSILQDEISHLKEDTLKKLEPGARLEGEVVEITDFGVFVNLGG"
BASE COUNT          156 a          316 c          366 g          232 t
ORIGIN      
        1 cgccgccgag gttgacgaac acgccgaagt ccgtgatctc gacgacctcg ccttcgaggc
       61 gggcgccggg ctcgagcttc ttgagcgtgt cttccttcag gtgggagatc tcgtcctgga
      121 ggatcgagcg ccgcgagatg atgacgcggt tgcgccggcg gttgagctcg atgatcttga
      181 ccttcatgcg gcggccgacg tacggcgcga ggtcgttcac cctgcggatg tcgacctggc
      241 tggccgggag gaaggcgcga acgccgaggc tggcgacgag gccgccgcgg accttctcga
      301 cgatctcgac ctcgacaggc ttgccgttct cgtggatgtc ctggaggagg ttccaggcgc
      361 gttcctggtc ggcgcgcttc ttggagagcg tgatggtgct gttggggatg tcggagcgga
      421 tgacgtacac ctggatgtcg tcgcccggct tgaagtactt ggcggcttcc tctgcgttcg
      481 actcgtgctc gaagagctgg ttgtagggca gcatgccctc gatcttcgcg ccgacgtcga
      541 cgacaatgcc ttcctgggcc agcatgatga cctgaccggt cgtgatcatg ccgcggtgga
      601 caggcttgcg cgccagctgt tcgtcgctgg ccgccagcac gtcttccatg gtgatctcgg
      661 cgtcagccga ggcgggggtt ggggcgggag ttgcagtctc ggaggaagac cctgcttgaa
      721 cggtgctcgt gttgtcagcc tgcgtgccta caccctggtc ggcggacgcg gctgaagcct
      781 ggttcgggct ctcttccggg gctgagacgg tgcctggggc agggctcccc gcggcggggt
      841 ttgcggtttc atccattgtt ttggggttcc tcctggaaat ggtgtcttat cgacaattgt
      901 tcattacacc acaagtggcg cccctaaagc aaggacgtgt cggcggccgg ctcctagacg
      961 cactcggcgt atcctcgatc atgagccatt tgggcttcca ccatgaacga tatggacttc
     1021 cacgccgtgc gtagcctcgc cgtgatcgcc gatatccatg gcaacatccc
//
LOCUS       DAOQYE010000937         1070 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_187981_length_1070_cov_2.809852, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000937 DAOQYE010000000
VERSION     DAOQYE010000937.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_187981_length_1070_cov_2.809852"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..624)
                     /locus_tag="PKN52_12380"
     CDS             complement(<1..624)
                     /locus_tag="PKN52_12380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNR00796.1"
                     /translation="MKDYDLITMGRSSIDLYSNDIGAPFEEITSFAAYVGGSPTNIAV
                     GANRLGLRTGLLTAVGEDKVGDFILAFLRREGVDVSFLPRKAGARSSAVVLGIEPPDR
                     FPLVFYRDNAADMQLTIDDVLAAPLERARALEVSGTGLSREPSRSATLFAAERAKASG
                     ATVFFDLDFRADQWHDPRAFGVCARSLLPNVHVAMGTEEEINAAMLSD"
     gene            complement(621..>1070)
                     /locus_tag="PKN52_12385"
     CDS             complement(621..>1070)
                     /locus_tag="PKN52_12385"
                     /inference="COORDINATES: protein motif:HMM:NF016826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HNR00797.1"
                     /translation="TPSGNWSSYPPHKHDVQALPDEVDLEEVYHYRFQPRDGWALQRL
                     YTDDRSLDEVVRAEDGDTVLVRQGYHPVVTAPGYDCYYLNFLAGDTPSWVARDEPRLA
                     WVRGNWEGAAERLRLPLGAASVADGTARADGVPTAGGKAVTPEGGAK"
BASE COUNT          153 a          365 c          393 g          159 t
ORIGIN      
        1 gtcgctcagc atggccgcgt tgatctcctc ctcggtgccc atggccacgt gcacgttggg
       61 caggagcgac cgggcgcaca cgccgaacgc gcgcggatcg tgccactggt cggcacggaa
      121 gtcgaggtcg aagaacacgg tcgccccgct cgccttcgcg cgctccgcgg cgaagagcgt
      181 ggcgctgcgg ctcggctcgc ggctcaggcc cgtgccggag acctcgaggg cccgagcgcg
      241 ctccaggggt gcggcgagca cgtcgtcgat ggtgagttgc atgtccgccg cgttgtcgcg
      301 gtagaagacg agaggaaagc ggtcgggcgg ctcgatgccg aggaccacgg cgctggagcg
      361 ggctccggcc ttgcggggca ggaagctaac gtccaccccc tcgcgcctca ggaacgcgag
      421 gatgaagtcg ccgaccttgt cctcgcccac ggccgtgagg agccccgtgc gcagcccgag
      481 gcggttggcg ccgacggcga tgttggtggg gctcccgccg acgtaggcgg cgaagctcgt
      541 gatctcctcg aacggcgcgc cgatgtcgtt ggagtagagg tcgatggatg agcgccccat
      601 cgtgatgagg tcgtagtcct tcatttggcg cccccttcgg gcgtcacggc tttcccgccg
      661 gcggtaggca caccgtcggc ccgggccgtg ccatcggcca ccgacgcggc cccaagcggc
      721 aaccgcaacc gctcggcggc gccctcccag ttgccgcgca cccaggcgag ccgcggctcg
      781 tccctcgcca cccacgacgg cgtgtcgccg gccaggaagt tcaggtagta gcagtcgtag
      841 ccgggcgccg tgacgacggg gtggtagccc tggcgcacga gaaccgtgtc gccgtcctcg
      901 gccctcacga cctcgtccaa ggaacggtcg tccgtgtaga ggcgctggag cgcccaaccg
      961 tcgcggggct ggaagcggta gtggtagacc tcctccaggt cgacctcgtc gggcagcgcc
     1021 tgcacgtcgt gcttgtgggg cggatacgag ctccagttgc cggagggagt
//
LOCUS       DAOQYE010000938         1068 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_188656_length_1068_cov_2.292201, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000938 DAOQYE010000000
VERSION     DAOQYE010000938.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_188656_length_1068_cov_2.292201"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..882)
                     /locus_tag="PKN52_12390"
     CDS             complement(<1..882)
                     /locus_tag="PKN52_12390"
                     /inference="COORDINATES: protein motif:HMM:NF015056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HNR00798.1"
                     /translation="MAHHVFKPTVYYNTFAEHPVALSVAPGDTVETTTVDAAGRDAAD
                     EQVTPRGNPMTGPFAVAGAEPGDAVTVRITRLEPNRDTGWASTRLAPNTLDPEFVAEF
                     RGEEGPARVTWRIDRERRRVVLDEGVAGLAGYSLPLTPMLGCLGVAPDRHQAISTATS
                     AEHGGNMDYRGIGVGVTLRFPVLAPGGLFFLGDGHAVQGAGEMAGTGVETSFDVGFQI
                     GLVKHDGRDQVRWPRGEDEHDLFTLGNARPLDQAAQNATTEMTRWLMNGYSLSFEAAS
                     VLIGQAAEYKIGNMFDPA"
BASE COUNT          166 a          369 c          374 g          159 t
ORIGIN      
        1 ggccggatcg aacatgttgc cgatcttgta ctcggcggcc tgcccgatga gcacggaggc
       61 cgcctcgaag ctcaggctgt aaccgttcat cagccagcgc gtcatctcgg tggtggcgtt
      121 ctgcgccgcc tggtcgagcg gacgggcgtt gccgagggtg aacaggtcgt gctcgtcctc
      181 gccgcgcggc cacctgacct ggtcgcgtcc gtcgtgcttg acgaggccga tctgaaagcc
      241 gacgtcgaag gaggtctcga cgcccgtgcc ggccatctcc cccgcgccct ggacggcatg
      301 cccgtcacct aggaagaaga ggccgccggg agcgagcacc gggaagcgca gcgtcacgcc
      361 tacccctatg ccacggtagt ccatgttgcc gccgtgctcg gccgacgtgg ccgtcgagat
      421 cgcctggtgg cgatcgggcg ccacgccgag gcacccgagc atgggcgtga gcggaaggct
      481 atagccggca agaccggcga ccccttcgtc gaggacgaca cgacgacgct cgcggtcgat
      541 gcgccaggtc acgcgcgccg ggccctcctc gccacggaac tcggccacga actccgggtc
      601 gagcgtgttt ggagccaacc gtgtgctggc ccagcccgtg tcgcggttcg gctcgagcct
      661 ggtgatacgc accgtgacgg catcgccagg ctcggccccc gccaccgcga aggggccggt
      721 catcgggttg ccccgtggcg tgacctgctc gtcggcggcg tcgcggccgg cggcgtcgac
      781 ggtcgtcgtc tccaccgtgt cgccgggcgc cacgctcagc gcgacgggat gctcggcgaa
      841 ggtgttgtag tagacggtcg gcttgaacac gtggtgagcc atgagcgaat catggcacca
      901 accgaccggg tatcgtccca ggtgcgcctg ccaggaagcc gcgtgcggcg cacgtgagga
      961 cgccagatga agacgcagta ctacacggca accagcctcg acggcttcat cgccaccgag
     1021 gacgactcgc tcgactggct gacgagcctc ggcgacccgg ccgagagc
//
LOCUS       DAOQYE010000939         1068 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_188808_length_1068_cov_1.499506, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000939 DAOQYE010000000
VERSION     DAOQYE010000939.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_188808_length_1068_cov_1.499506"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /locus_tag="PKN52_12395"
     CDS             <1..597
                     /locus_tag="PKN52_12395"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNR00799.1"
                     /translation="GPTGTSLAAKSRAVSVAAPRQPEAIKGDAFVLAAGAWTGRLARL
                     LDMHVPMQPGKGYSLTLERPSQRLAVPSILTEARVAVTRMGEKLRVGGTMELSGFDER
                     ANELRVQGIVEAALRYFPGLSASEFQAAPRWHGFRPLSPDGLPYLGLARRYPNLVVAS
                     GHGMMGLSLAPVTGKLVGELVAGEPTSLDVRALAVERF"
     gene            697..936
                     /locus_tag="PKN52_12400"
     CDS             697..936
                     /locus_tag="PKN52_12400"
                     /inference="COORDINATES: protein motif:HMM:NF015947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HNR00800.1"
                     /translation="MNTKATITSKGQVTIPKAVRARLGLEQGDVVEFVTLEGLTYLKP
                     VRSGDNPFAAYAGALPAFETRDEIGTWLRDLREDD"
BASE COUNT          160 a          341 c          404 g          163 t
ORIGIN      
        1 ggtccgaccg gaacgagcct cgcagcgaaa agccgcgccg tgagcgtggc ggcgccccgc
       61 caaccggaag ccatcaaggg cgacgccttc gtgctcgccg ccggcgcgtg gaccggccgg
      121 ctggcgcgcc tactcgacat gcacgtgccc atgcagcccg gcaagggtta cagcctcacg
      181 ctcgagcggc cgtcgcagcg tctcgcagtg ccgtccatcc tcacggaggc gcgcgtggcc
      241 gtgacccgca tgggcgagaa gctgcgggtg ggcggcacca tggagcttag cggcttcgac
      301 gagcgcgcca acgagctgcg ggtgcaaggg atcgtggagg cggcgctccg gtacttcccc
      361 gggctgtcgg cgagcgagtt ccaggcggcc ccgcgctggc acggcttccg gccgctctcg
      421 ccggacggcc tgccgtacct cggcctcgcc aggcgctacc ccaacctggt cgtggcctcc
      481 ggccacggga tgatggggct gagcctggcg cccgtcacgg gcaagctggt gggggagctg
      541 gtggcgggcg agccgacgag cctcgacgtg cgggcgttgg cggtggagag gttctgacgg
      601 agcagctcga ggccggtggt tgcgcgggtg cgtccttggt aagacctgtc ttcggcaccg
      661 ccgcgaggta tccttacttc ttggaggcga agtaaggtga acacgaaggc caccatcacg
      721 agcaaggggc aggtcaccat ccccaaggcg gtccgtgccc ggctcggtct ggaacagggc
      781 gacgtcgtcg agttcgtgac gttggaaggg ctcacgtacc tgaagcccgt gcgcagtggc
      841 gacaaccctt tcgccgccta cgcaggggcg ctgccagcgt tcgagaccag ggacgagatc
      901 ggtacttggt tgcgtgacct cagggaagac gactgagggt gcgaagcgct gtagacacga
      961 acgtgatctc ggccctttgg tcggccgagc cgttgggcgg cgccatggct ggatggctgg
     1021 acgaggcgag gcgtgccggc ggcctggtca tctgcgcgcc gatgttgg
//
LOCUS       DAOQYE010000940         1067 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_188959_length_1067_cov_2.304348, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000940 DAOQYE010000000
VERSION     DAOQYE010000940.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1067
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_188959_length_1067_cov_2.304348"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="PKN52_12405"
     CDS             <1..415
                     /locus_tag="PKN52_12405"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNR00801.1"
                     /translation="KVKVADPHATDRHATDRHATERHATDPHATNPHLSERDLEAVKL
                     PEDLVEEAVRLARGFADPTRLRILALLRRGEVCVHQIVTALAMEQSAVSHQLRVLRQA
                     RLVTGAKRGRHVYYRLADEHVASMLEGVLSHSQEA"
     gene            591..>1067
                     /locus_tag="PKN52_12410"
     CDS             591..>1067
                     /locus_tag="PKN52_12410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00802.1"
                     /translation="MTVTSLDLQSLLPGHAPREDACTARLVAAVTALRGVVSADFRTD
                     GRRSALSVEHDPAVVDRDELSALARDAALAVSTRFVHRELLLSGLHCSDCAAKVATVT
                     RKLDGVFEADASFATEKLHLGLDTATSAASEMLSRVASEVEGLGYRVVRDGSGAATL"
BASE COUNT          168 a          393 c          364 g          142 t
ORIGIN      
        1 gaaggtgaag gtggcggacc cgcacgcgac agatcgacat gcgaccgacc gacatgcgac
       61 cgagcgacat gcaaccgacc cgcacgcgac gaacccgcac ctgagcgagc gcgacctcga
      121 ggcagtcaag ctgccggagg acctggtcga ggaggccgtg cgcctagccc gcggcttcgc
      181 ggacccgacc aggctccgca tcctcgcgct gctgcggcgc ggcgaggtgt gcgtccacca
      241 gatcgtcacg gccctcgcca tggagcagtc ggcggtgagc caccagctcc gcgtgctgcg
      301 ccaggcgcgg ctcgtgacgg gcgcgaagcg cggtcgccac gtgtactacc ggctcgccga
      361 cgagcacgtc gcgagcatgc tggagggcgt cctctcgcat agccaggagg cctgaggccc
      421 ctaacgccgg gggcgggagc tgggaaaccg cggtagctcg gcaagccgct ggccagtaac
      481 gctccggctc gaccacgccc ggtggccgcc ctccgcagtc gccacatatg aacagtcatt
      541 catatatact cgcctagggg atctcgccta gggggatgga gccaactgcc gtgaccgtta
      601 cctccctcga cctacagagc ctcctcccgg ggcacgcccc gcgcgaggac gcgtgcaccg
      661 cgcgcctggt cgcggccgtg acggccctgc gcggagtcgt ctccgcggac ttccgcaccg
      721 acggcaggcg gagcgcgttg agcgtcgagc acgaccccgc ggtcgtcgac cgagacgagc
      781 tctccgctct cgcccgcgac gccgcgctcg cggtctctac gcgcttcgtc caccgggaac
      841 tcctcctcag cggcctgcac tgctccgact gcgcggccaa ggtcgcgacc gtgacgcgca
      901 agctggacgg ggtgttcgag gccgacgcca gcttcgcaac cgagaagttg catctcgggc
      961 tcgacacggc gacctcggcg gcgtccgaga tgctgtcacg cgtcgcgtcg gaagtcgagg
     1021 gcctcggcta ccgcgtcgtg cgagacggct ccggcgcagc gaccctc
//
LOCUS       DAOQYE010000941         1066 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_189192_length_1066_cov_2.812067, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000941 DAOQYE010000000
VERSION     DAOQYE010000941.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_189192_length_1066_cov_2.812067"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="PKN52_12415"
     CDS             complement(<1..274)
                     /locus_tag="PKN52_12415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00803.1"
                     /translation="MSIASPSTPRLTVAAVHYRTPELAVSCLERVRRSPPQAELRLVD
                     TDPDEAFARALAKERPDVAYLGAANRSYAHAVNVGLAGATTEYVAFL"
     gene            373..>1066
                     /locus_tag="PKN52_12420"
     CDS             373..>1066
                     /locus_tag="PKN52_12420"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNR00804.1"
                     /translation="MQSADTHDTGGELRLGVALGGGSARGYAHLGALASLERNGFAPA
                     VIAGTSFGAVVGALYATGRPLSELLAQAEAMRRRDVFPYVADFGLHRAALFKGRRLEA
                     YFDRLLEGRHFSDLVKRLVVVTTDIDTGERVLLEHGSLAEALRASTAIPGVFAPALVG
                     GRRLIDGGIGSPVPLSTLDEFDLDVAIGIGAGMEAADSGTIRFARKLMSSDGARRVQT
                     RLATARPRGAVGR"
BASE COUNT          149 a          379 c          381 g          157 t
ORIGIN      
        1 tcaagaaggc cacgtactcg gtggtcgcgc cggcgagccc cacgttcacg gcgtgggcgt
       61 acgaccggtt ggcggcacct aagtaggcca cgtcgggtcg ctccttggcg agggcgcgag
      121 cgaacgcctc gtcgggatcg gtgtcgacga gccgcaactc cgcctggggc ggcgagcgcc
      181 gcacgcgctc gaggcacgag acggcgagct ccggcgtccg gtaatgaacc gcggcgacgg
      241 tcagccgggg cgtgctcggc gaagcgatgc tcacctcggt cccttcgtgg agcggcaggt
      301 cacgagcgca tgctactcaa gagcgggcct agaccacaca tgtctcacgt cgcctggctt
      361 agcatgcggg gcgtgcagtc agcagataca cacgataccg gcggcgagtt gcggctcggc
      421 gtcgccttgg gcggcgggag cgcgcgcggc tacgcccatc tcggagcgct ggccagcctc
      481 gagcgcaacg gcttcgcgcc cgccgtcatc gcgggcacga gcttcggcgc cgtcgtcggc
      541 gccctctacg ccacgggcag gcccttgtcc gagctcctcg cccaggcgga agccatgcgc
      601 aggcgcgacg tcttcccgta cgtggccgac ttcggcctgc accgcgcggc cctgttcaag
      661 ggtcggcggc tcgaggcgta cttcgacagg ctactggagg gtcgacactt ctccgaccta
      721 gtgaaacgcc tcgtggtggt cacgaccgac atcgacacgg gcgagcgcgt gctgctcgag
      781 cacggctcct tggcggaagc gttgcgggcc agcacggcca tccccggggt gttcgccccc
      841 gcgttggtcg gcggtcggcg gctcatcgac ggcggcatcg gctctcccgt cccgctgtcg
      901 accctcgacg agttcgacct cgacgttgcc atcggcatcg gcgccggcat ggaagcggcc
      961 gactccggca cgatccgctt cgcgcgcaag ctgatgagct cggacggtgc caggcgcgtc
     1021 cagacccgcc tcgccaccgc caggccgcgc ggagcggtcg ggcggc
//
LOCUS       DAOQYE010000942         1066 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_189251_length_1066_cov_2.273986, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000942 DAOQYE010000000
VERSION     DAOQYE010000942.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_189251_length_1066_cov_2.273986"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>284
                     /locus_tag="PKN52_12425"
     CDS             <1..>284
                     /locus_tag="PKN52_12425"
                     /inference="COORDINATES: protein motif:HMM:NF021375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2075 domain-containing protein"
                     /protein_id="HNR00805.1"
                     /translation="NLFKGSGAYVDTEPNTFDALIVDEAHRLNEKSGLYGNLGENQIS
                     ETIRAAKATVFFIDEAQRVTFKDIGTVRTIQALADAEVAEVTIGRLESQFR"
     assembly_gap    285..294
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <295..961
                     /locus_tag="PKN52_12430"
     CDS             <295..961
                     /locus_tag="PKN52_12430"
                     /inference="COORDINATES: protein motif:HMM:NF021375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2075 domain-containing protein"
                     /protein_id="HNR00806.1"
                     /translation="AQVIEDELASQFRCNGSDGYLAWLDDVLQVRPTANDDVSDLNYE
                     FEVVDSPSVLRDNIIDANIANNKSRMVAGYCWDWRSKKDPYAYDLEFPEYEFAAQWNL
                     SEDGSLWILKPNSVEQVGCIHTCQGLELDYVGVIIGPDLVVRGGRVTTSGFARSRMDS
                     TIKGFKRMHRNAPAHAAVLADEIIKNTYRTLMTRGTKGCFVWSPDAETREYFRLRSLR
                     GVD"
BASE COUNT          212 a          348 c          331 g          165 t
ORIGIN      
        1 aacctcttca agggctctgg cgcgtacgtc gatacggagc cgaacacgtt cgacgccctc
       61 atcgtggacg aggcccatcg cctcaacgag aagtccggcc tatacggcaa cttgggcgag
      121 aaccagatct ccgagacgat ccgcgccgcc aaggccacgg tcttcttcat cgacgaggcc
      181 caacgcgtca ccttcaagga catcggcacg gtgagaacga tccaggcgct ggccgacgcc
      241 gaggtcgccg aggtgacgat cgggcgccta gagtcgcagt tccgnnnnnn nnnnggccca
      301 agtcatcgag gacgaactgg caagccagtt ccggtgcaac ggttccgacg gctacctggc
      361 gtggctcgac gacgtgctgc aggtcaggcc gactgccaac gacgacgtct cggacctgaa
      421 ctacgagttc gaggtcgtcg actcgccctc cgtcctgcgc gacaatatca tcgacgcgaa
      481 catcgccaac aacaagtccc gcatggtcgc gggctactgc tgggactggc gcagcaagaa
      541 agacccctac gcgtacgacc tggagttccc cgagtacgag ttcgccgcgc agtggaacct
      601 ctccgaggac ggcagcctct ggatcctcaa gcccaactcg gtcgagcagg tcggttgcat
      661 ccacacgtgc cagggcctcg agcttgacta cgtcggcgtg atcatcggcc cggacctcgt
      721 ggtgaggggc ggacgcgtga cgacgagcgg cttcgcccgc tcccggatgg actcgaccat
      781 caaagggttc aagcgcatgc accggaacgc tcccgcgcac gcggcagtcc tcgcggacga
      841 gatcatcaag aacacgtacc gcacgctcat gacccgcgga acgaaaggct gcttcgtctg
      901 gagtccggac gccgagacgc gcgagtactt cagactcagg tcgttgcggg gggtcgactg
      961 aggcaccccc ctccagggtg cggcctaccg aggctgcgta ctgctgaatg aaccgagcgc
     1021 gttcaggagc gacgacgatc cgaggtcttc ctagcgttac ggggcg
//
LOCUS       DAOQYE010000943         1066 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_189413_length_1066_cov_1.288823, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000943 DAOQYE010000000
VERSION     DAOQYE010000943.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_189413_length_1066_cov_1.288823"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(240..>572)
                     /locus_tag="PKN52_12435"
     CDS             complement(240..>572)
                     /locus_tag="PKN52_12435"
                     /inference="COORDINATES: protein motif:HMM:NF025793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HNR00807.1"
                     /translation="GRGHNRRVQDGDPSSHGETDAFRNAGRQRSYKDTIMVTTLEPCW
                     YCSGLIKQFGIGTVVVGESRTFKGGAEWLKEQGVNVIDLDDPACVQLMTEFIAAKPEL
                     WNEDIGEE"
     assembly_gap    573..582
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<583..766)
                     /locus_tag="PKN52_12440"
     CDS             complement(<583..766)
                     /locus_tag="PKN52_12440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00808.1"
                     /translation="MAKPLDHQAMLSVALEEARQGLAEGGIPIGAALFRSDGTLLVQG
                     AGSTLSMVRSLTHDAQS"
BASE COUNT          146 a          393 c          345 g          172 t
ORIGIN      
        1 taaggctcga gcccgaggcc agccagccgc cccgtcaagt acctcttcgc ggcggcgtga
       61 cccgcagagc cgacctggcg gtcgcctgcg cccgcgagcg ccgcgacgtc ggcgcgaagc
      121 gtgacatcgc tcatccctcg atcatgcctg gtgggggtgc cgcgtccgtg ccgacggcgc
      181 cacacgtcgg gccgggcgcc gggtaggcct cgctcgcgca tagcgacgac caaccagggt
      241 cactcctcgc cgatgtcctc gttccagagc tcgggcttgg cggcgatgaa ctccgtcatc
      301 agttgcacgc acgcggggtc gtcgaggtcg atgacgttca cgccctgctc cttgagccac
      361 tcggcgcccc ccttgaaggt gcgcgactct ccgaccacca cggtgccgat tccgaactgc
      421 ttgatgaggc cgctgcagta ccagcacggc tcgagcgtgg tgaccatgat cgtgtccttg
      481 tagctccgtt gccggccggc gttgcggaac gcgtccgtct cgccgtggct gctcgggtcg
      541 ccgtcttgca cgcgacggtt gtggccgcgc ccnnnnnnnn nntgctctgt gcatcatgcg
      601 tgagcgaacg caccatcgag agcgtcgatc cggcgccctg aaccaacagc gtgccgtcgg
      661 agcggaagag ggcggcgccg atggggatgc ccccctccgc cagcccctgc cgggcctcct
      721 cgagcgcgac gctcagcatc gcctggtggt ctaacggctt ggccatggct gcctccctcg
      781 ttccgcgccc gctggcgacg gcgtccccgc cccgcgcggc tcgcgcctga ctcaaggcta
      841 cccccctcgc cccctcgcgg ggcgaacccc ctgctatact ccccgaaaac cagttgggtg
      901 ccggccaccg gtcggcagga agggcgtgaa catgaagagg ctcgcggtcg ggatcctact
      961 ggctattgcg acgttgttcg cctacgcgaa cgcccaggcg cccgtcaagg tctacgcggt
     1021 gtacgccacc cccatcgagg agccgtggga cggggtcatc cacgcc
//
LOCUS       DAOQYE010000944         1064 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_190056_length_1064_cov_1.115956, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000944 DAOQYE010000000
VERSION     DAOQYE010000944.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_190056_length_1064_cov_1.115956"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /gene="glyA"
                     /locus_tag="PKN52_12445"
     CDS             complement(<1..294)
                     /gene="glyA"
                     /locus_tag="PKN52_12445"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176652.1"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HNR00809.1"
                     /translation="MANSAQPERDERIFELIDQEFDRQAGGLELIASENFVSRQVMEA
                     VGSVLTNKYAEGYPGKRYYGGCEVVDQVEQLAIDRAKALFRAGWANVQPHSGSN"
     gene            complement(333..>1064)
                     /locus_tag="PKN52_12450"
     CDS             complement(333..>1064)
                     /locus_tag="PKN52_12450"
                     /inference="COORDINATES: protein motif:HMM:NF014038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HNR00810.1"
                     /translation="ARRLHSAMPRELELKYSSLEGRVPGVAELAGALAPLGLQVEGRG
                     TRRQEDVYYDDAEGTLAAAGLALRVRAIGCAGAVGGAGAVGRARVVAVKGRGDAVAGL
                     FEREELEAPLAGPAAGRGGAADAAVDWPAPIAARLAGVVDLPALAPVLEIVTLREVFT
                     LGRAGEPVAEVAFDEVTCRPPRAGAAGALIEEAAFSEVEVEALGEGTALELRRVGAAL
                     EALLPLVAGDVSKLERASALLAPFL"
BASE COUNT          134 a          420 c          377 g          133 t
ORIGIN      
        1 gttcgagccg gagtgcggct gcacgttcgc ccaaccggcg cggaagagcg ccttggcgcg
       61 gtcgatggcg agctgctcca cctggtcgac gacctcgcag ccgccgtagt agcgcttgcc
      121 cgggtacccc tcggcgtact tgttggtgag gacggagccg acggcctcca tgacctgcct
      181 cgagacgaag ttctccgagg cgatcagttc gaggccgccg gcctgccggt cgaactcctg
      241 gtcgatgagc tcgaagatgc gctcgtcgcg ttcgggttgc gcgctgttcg ccatgcccga
      301 gtatagcctc gggggtgccg cgctcccgcg ccctacagga agggcgccag cagcgccgag
      361 gcgcgttcga gcttgctcac gtcgccggca acgagcggca gcagcgcctc cagggcggct
      421 cccacccggc gcagctccag agcggtgccc tcaccgagcg cctcgacctc gacctcggag
      481 aaggcggcct cctcgatgag cgcgcctgcc gcgccggcgc ggggcggcct gcaggtcacc
      541 tcgtcgaagg cgacctcggc gaccggctca ccggcccggc cgagcgtgaa gacctcgcgc
      601 agcgtgacga tctctaggac gggggcgagg gcgggcaggt cgacgacgcc cgccagccgc
      661 gcggcgatcg gcgcgggcca gtcgacggcg gcgtccgcgg cgccaccccg ccctgcggcc
      721 ggcccggcga gcggcgcctc gagctcctcc cgctcgaaga gccccgccac ggcgtcgccg
      781 cggcccttca ccgccacgac gcgcgcgcgg cccacggcgc ccgcgccgcc cacggcgccc
      841 gcgcagccta tggcgcgcac tcgcagggcg aggccggccg cggccagcgt gccctcggcg
      901 tcgtcgtagt agacgtcttc ctggcgcctc gtcccgcgcc cctcgacctg caggccgagc
      961 ggtgccagcg cccccgccaa ctcggcgacc cccggcacgc gcccctccag cgaggagtac
     1021 ttgagttcca gttcgcgcgg catggccgag tgtaaccgcc gcgc
//
LOCUS       DAOQYE010000945         1063 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_190157_length_1063_cov_2.388889, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000945 DAOQYE010000000
VERSION     DAOQYE010000945.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_190157_length_1063_cov_2.388889"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(19..>1063)
                     /locus_tag="PKN52_12455"
     CDS             complement(19..>1063)
                     /locus_tag="PKN52_12455"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNR00811.1"
                     /translation="GNLLKPLNAVAGSAVDSWEGWNHIAPGIQSILSYGGEIYGVANG
                     TDVRMIFYRKDILAQAGVANADAWQPTSWADIADAAAKVLAAFPDSAPLQINAGTSMG
                     EATTLQGYYMLLLGTGEEPFMDGKWVVRSQGILDTLNFYREIYVDHAYGSQRVQLIND
                     GRNLSFANFRDGKTAMLVEGDYFYRSVTPAGSEFAVENRDQVMGWAKMPSETPGSGIR
                     GQDFVSASGGTGWVLNPNSANPQLAWEFLAFMNSKAARDVWMTYQPGIPARDDVPVPN
                     NAFLTDTAAALLPLTVSRPNNDFYAKVSEQIQLMTENVVSGQMTPQQAMDAYAAAVTG
                     IVGADATVDLLAR"
BASE COUNT          152 a          316 c          404 g          191 t
ORIGIN      
        1 cccgctgggt ccggtgactt agcgggccaa caggtcgacg gtcgcgtcgg cgccgacgat
       61 gccggtgacg gcggccgcgt aggcgtccat cgcttgctgc ggcgtcatct ggccggaaac
      121 gacgttctcg gtcatgagct ggatctgctc ggagaccttg gcgtagaagt cgttgttcgg
      181 gcgcgagacg gtgaggggca ggagcgccgc cgccgtgtcg gtcaggaagg cgttgttcgg
      241 cacggggacg tcgtcgcggg ccggtatgcc gggctggtac gtcatccaga cgtcgcgcgc
      301 ggccttgctg ttcatgaacg cgaggaactc ccaagcgagc tgcgggttgg cggagttggg
      361 gttgagcacc caaccggtgc cgcccgaggc gctgacgaag tcctggccgc ggatgccgga
      421 gccgggggtc tcgctcggca tcttggccca gcccatgacc tgatcgcggt tctcgacggc
      481 gaactcggag ccggcaggcg tgaccgagcg gtagaagtag tcgccttcga cgagcatggc
      541 ggtcttgccg tcacggaagt tggcgaacga caggttgcgg ccgtcgttga tgagctgcac
      601 gcgctgcgag ccgtaggcgt ggtcgacgta gatctcgcgg tagaagttca gcgtgtcgag
      661 gatgccctgg ctgcggacga cccacttgcc gtccatgaac ggctcttcgc ccgtgccgag
      721 gaggagcatg tagtagccct gcagcgtggt cgcctcaccc atgctcgtgc cggcgttgat
      781 ctgtagcggc gcgctgtcgg ggaaggcggc gaggaccttg gcggccgcgt ccgcgatgtc
      841 ggcccatgac gtgggctgcc aggcgtcggc gttggcgacg ccggcctgcg cgaggatgtc
      901 cttgcggtag aagatcatgc ggacgtccgt gccgttggcg acgccgtaga tctcgccgcc
      961 gtaactcagg atgctctgga tgccgggggc gatgtggttc cagccttccc agctatcgac
     1021 ggccgagccg gccacggcgt tcaagggctt gaggaggttg ccg
//
LOCUS       DAOQYE010000946         1063 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_190266_length_1063_cov_1.716270, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000946 DAOQYE010000000
VERSION     DAOQYE010000946.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_190266_length_1063_cov_1.716270"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            492..893
                     /locus_tag="PKN52_12460"
     CDS             492..893
                     /locus_tag="PKN52_12460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00812.1"
                     /translation="MRRTLATILLLGLLTAAPALARDNVEASPYGMVVSDLLLSFHAN
                     EVPCEDGAVEDPEVCFMVASVGASYLAERLTTLVESYAPAGLSQGEWRAANGVWAVSL
                     AFRNDSYGRLDVYLAETLGSGVRGMVRLVQR"
BASE COUNT          148 a          403 c          351 g          161 t
ORIGIN      
        1 catgcgctcg agctcctcac gccgggtcga ctccggacct aggagctctt cgcgtcgacc
       61 cggggccctg gccgcgtcgg tcgcgccctc gtctcccgtg cccacctcgt ctcgctcgct
      121 gtccggtcgg ctcactcgcc cacgactcct cttccgactc ctcttccgac tcctctgcga
      181 cacctggcgc gtcgttggcc tcggcgccgg ttgcggcgat tgtatcgggc ggctccgtgc
      241 gcggccgtcg cggcccggcg ccggccgaca ggcaccggtc cacggccatc agacaccagg
      301 cacgggccta gcagctctag gccccggact acgagcttta cgctgcagcc tacgcctcag
      361 ccaacgggct ctacaccctg gactactagc tctacacccc ggccgggcgc gcatacacat
      421 caaacgacct cgccccggat gcgaagatgg ccggcgaaga ggaagagggc gccacgcccg
      481 cctgtgagcg catgagaaga accctggcca ccatcctcct actgggcctc ctgacggccg
      541 cgcccgcgct ggcgcgcgac aacgtggagg ccagccctta tggcatggtc gtcagcgacc
      601 tcctcctgag cttccacgcg aacgaggtgc cctgcgagga cggcgccgtc gaggacccgg
      661 aggtctgctt catggtggcg agcgtcggcg cctcgtacct ggctgaacgc ctcacgacgc
      721 tcgtggagag ctacgcgccg gccgggctgt cgcagggcga gtggcgcgcc gccaacgggg
      781 tgtgggcggt gtcgctagcg ttccgcaacg atagctacgg gcgcctcgac gtctacctgg
      841 ccgagactct cggctccggc gtccgcggca tggtgcgcct ggtccagcgc tgagggtcgc
      901 gccgccggac ccacggagcc aagcgtcgcg ccgccgggtc agccttcgcc gctcacggcc
      961 gtctccgtcg cgctacgcgg gctggtggcg aaggccctga tggtcacgac cagcgccacc
     1021 gcgagcaggg cagccagcac gagcaggtag atcagcgggc ttc
//
LOCUS       DAOQYE010000947         1061 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_190801_length_1061_cov_2.101392, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000947 DAOQYE010000000
VERSION     DAOQYE010000947.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_190801_length_1061_cov_2.101392"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            10..>1061
                     /locus_tag="PKN52_12465"
     CDS             10..>1061
                     /locus_tag="PKN52_12465"
                     /inference="COORDINATES: protein motif:HMM:NF019869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4040 domain-containing protein"
                     /protein_id="HNR00813.1"
                     /translation="MLHCLEVFLQLLSPFMPFLSEEIWQMLPGRSGESITLGPWPEAD
                     AAMIDDEVEARSLLKREAVTAVRNLRKAYAVPARAGVAVVAVLGPEAPRFHLELWHGF
                     TPAFMLSLVAMALGASLYFALYRRLANREARERPWAGRARARRIFDGAMTGLFAGAGF
                     ITRLAGTVRLQPQLFLIVLAAVVAGAAPFVYHAVTQAGAGLTLGTRPLTPVSLPFVVV
                     WAFGIASATGAAILAKFHRLAALILTGGAGLAVCLTFVWLSAPDLAVTQLLVEIVTTV
                     LLLLGLRWLPARVATKEDRERARALVVARRGRDLVLALIAGGGMATLALAAMTRPRVG
                     GIAGFFNANALEGGGGR"
BASE COUNT          115 a          404 c          368 g          174 t
ORIGIN      
        1 cgccggacga tgcttcactg cctcgaggtc ttcctgcagc ttctctcgcc gttcatgccg
       61 ttcctgagcg aggagatctg gcagatgctt cccgggcggt ccggcgagtc gataacgctg
      121 ggcccgtggc cggaggcgga cgcggccatg atcgacgacg aggtcgaggc gcggtcgctg
      181 ctcaagcgcg aggcggtcac ggcggtgcgc aatctccgca aggcgtacgc cgtcccggcg
      241 cgcgcggggg tggcggtcgt cgcggtgctc ggtccggagg ctccgcgctt ccacctcgag
      301 ctgtggcacg ggttcacgcc agcgttcatg ctcagcctcg tagcgatggc gctcggcgcc
      361 agcctttact tcgccctgta ccgccgcctc gccaaccgcg aggcccgcga gcggccgtgg
      421 gcgggccgcg cgcgcgcgcg gcgcatcttc gacggggcca tgaccggctt gttcgccggc
      481 gccggcttca tcacgcgcct ggccggcaca gtaaggctcc agccgcagct cttcctcatc
      541 gtcctcgccg cggtcgttgc cggcgcagcc ccgttcgtct accacgccgt tacccaagcc
      601 ggcgcaggcc tgacgctcgg gactcggccc ctcacccccg tctccttgcc gttcgtggtc
      661 gtgtgggcgt tcgggatcgc aagcgccacg ggcgcggcca tcctcgccaa gttccaccgg
      721 ctcgccgccc tcatcctcac gggcggcgcc ggcttggccg tatgcctcac cttcgtctgg
      781 ctctcggcgc ccgacctggc cgtcacgcag ctcctcgtcg agatcgtgac caccgtgctc
      841 ctcctgctcg gcctgcgctg gctcccagcg cgcgtggcca ccaaggagga ccgggagcgc
      901 gccagagccc tggtcgtcgc gcgccgcggc cgcgacctgg tcctggcgct gatcgccgga
      961 ggcggcatgg cgaccctcgc cctggcggcc atgacgcgcc cgcgggtcgg cggcatagcg
     1021 gggttcttca acgccaacgc gctcgagggt gggggcggcc g
//
LOCUS       DAOQYE010000948         1060 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_191069_length_1060_cov_2.331343, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000948 DAOQYE010000000
VERSION     DAOQYE010000948.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_191069_length_1060_cov_2.331343"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..674
                     /locus_tag="PKN52_12470"
     CDS             <1..674
                     /locus_tag="PKN52_12470"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNR00814.1"
                     /translation="KEIKVEDTALRQLADAASIDPDATVLATPDIHSGYGVPIGCVWA
                     TPHFISPAAVGYDINCGMRLMTTPLGKADFILGYALAFGLMAMLEAVITVTFAVWVCG
                     LDVAGSLWQMMLVAVVAAVLGTALGLLASAFARTEFQAVQFMPVLVFPQVLLGGLFMP
                     RDLMPDALRVISDWLPLSYAIDAVTAVRVGEGGWGLWRPLLVVVAFGVACLALASVTL
                     RRRTP"
     gene            730..>1060
                     /locus_tag="PKN52_12475"
     CDS             730..>1060
                     /locus_tag="PKN52_12475"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNR00815.1"
                     /translation="MPSTATFEAIAEPTRRRILDAVRERERSVGDLVEIVGMHQPGVS
                     RHLRVLRDAGLVEVRRQAQRRLYRLRPEPLRELNDWLEPYRMELTQRLDALEQHLERT
                     APRKKETP"
BASE COUNT          156 a          354 c          350 g          200 t
ORIGIN      
        1 gcaaggagat caaggtcgag gacacggccc tccgccagct ggccgacgcc gccagcatcg
       61 accccgacgc cacggtcctg gccacgcccg acattcacag cggatacggc gtgcccatcg
      121 gctgcgtctg ggccacaccg cacttcatct cgcccgccgc cgtcggctac gacatcaatt
      181 gcggcatgcg gctcatgacc acgccgctcg gcaaggccga cttcatcctc ggctacgcgc
      241 tcgcgttcgg cctcatggcc atgttggagg cggtcatcac cgtgaccttc gcggtttggg
      301 tgtgcgggct ggacgtcgcg gggtcgctat ggcagatgat gctcgtggcc gtggtcgcgg
      361 cggtcctcgg gacggcgctc gggctgctgg ccagcgcctt cgctcgcacc gagttccagg
      421 ccgtgcagtt catgcccgtc ctcgtcttcc cgcaagtgct cctcggcggc ctcttcatgc
      481 cgcgcgacct gatgccggac gctctccgcg tcatcagcga ctggttaccg ctcagctacg
      541 ccatcgacgc ggttactgcc gtcagggtgg gggagggcgg ctgggggctg tggcggccgc
      601 tgctcgtcgt cgtggctttc ggagtggcgt gcctcgcgtt ggcctcggtg acgttgcgta
      661 ggcgcacgcc ttgaggtttg actttatatt tgcttacaca tatatatgag atgacgtata
      721 cttgacgatg tgccatcaac cgccaccttc gaagctatcg ccgagccgac ccgccgccgc
      781 attctcgatg ccgtccgcga gcgtgagcgc tctgtcggcg atctcgtgga gatcgtagga
      841 atgcaccagc ccggcgtctc caggcacctg agggtgctgc gtgatgcggg gctggtcgag
      901 gtccggcggc aggcgcagcg gaggctctac cgcttgcgtc cggagcctct cagggaactg
      961 aacgactggc tcgagccgta ccggatggag ttgacgcagc ggctggatgc cctcgaacaa
     1021 cacctggagc gcaccgcccc ccggaagaag gagacgccat
//
LOCUS       DAOQYE010000949         1059 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_191335_length_1059_cov_2.697211, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000949 DAOQYE010000000
VERSION     DAOQYE010000949.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_191335_length_1059_cov_2.697211"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..145)
                     /locus_tag="PKN52_12480"
     CDS             complement(<1..145)
                     /locus_tag="PKN52_12480"
                     /inference="COORDINATES: protein motif:HMM:NF012462.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0042255 - ribosome assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uL22 family ribosomal protein"
                     /protein_id="HNR00816.1"
                     /translation="MLRVTPRKTRLVADLIRGKDVSKAEDILRFTDKRSAKPMLKVLQ
                     SAKA"
     gene            complement(178..447)
                     /gene="rpsS"
                     /locus_tag="PKN52_12485"
     CDS             complement(178..447)
                     /gene="rpsS"
                     /locus_tag="PKN52_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HNR00817.1"
                     /translation="MARSLKKGPFVDGHLLKKIDAANASGDRRVIKTWSRRSTVVPEM
                     VGHTIGVYNGRQHVPVFVQETMVGHKLGEFSPTRSFRGHSGSRKE"
     gene            complement(450..>1059)
                     /gene="rplB"
                     /locus_tag="PKN52_12490"
     CDS             complement(450..>1059)
                     /gene="rplB"
                     /locus_tag="PKN52_12490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HNR00818.1"
                     /translation="PAKVASIEYDPNRSANIALLHYVDGEKRYVLAPDKLTVGSSVVA
                     GADAEPVLGNAMPLRFIPVGAVVHAVELLPGKGAQLARSAGTSIQIQGRDSSYVTLRL
                     PSGELRKVHGECYATIGVVGNSDHKNVVLGKAGRTRWLGRKPHQRGRAMNPVDHPHGG
                     GEGRSTGGRPPVSPWGQKAKGLKTRDKRKGSSRFIVRRRKGS"
BASE COUNT          134 a          341 c          401 g          183 t
ORIGIN      
        1 tcgccttggc gctctggagc accttgagca tgggcttggc ggagcgcttg tccgtgaagc
       61 gcaggatgtc ctccgccttg ctcacgtcct tgccgcggat gaggtcggcc acgaggcgcg
      121 tcttgcgcgg ggtgacgcgc aacatgcgca gggaggcctt ggcttgctga gccacggtca
      181 ctccttccgc gagccggagt ggccgcggaa cgagcgggtc ggcgagaact cgccgagctt
      241 gtgcccgacc atggtctcct ggacgaagac ggggacgtgc tgccggccgt tgtagacgcc
      301 gatggtgtgc ccgaccatct cggggaccac cgtgctgcgg cggctccagg tcttgatcac
      361 gcggcggtcg ccggaggcgt tggctgcgtc gatcttcttg agcaggtgac cgtcgacgaa
      421 cggtcccttc ttgaggctac gcgccattgt cagctcccct tccggcgccg cacgatgaag
      481 cggctggaac ccttgcgctt gtcgcgggtc ttgaggccct tggccttctg gccccacggg
      541 ctgacgggcg ggcggccacc ggtggagcgc ccttcgccac cgccgtgcgg gtggtcgacc
      601 gggttcatgg cgcgaccgcg ctggtgcggc ttgcggccga gccaccgggt gcgaccggcc
      661 ttgccgagca cgacgttctt gtggtccgag ttgccgacga cgccgatggt ggcgtagcac
      721 tcgccgtgga ccttgcggag ctcgccggac ggcaggcgca gggtgacgta cgaggagtcg
      781 cggccctgga tctggatgct cgtgccggcg gagcgcgcga gctgcgcgcc cttgccgggc
      841 agcagctcca cggcgtgcac gaccgcgccg accgggatga accgcagcgg catggcgttg
      901 ccgagcacgg gctcggcgtc ggcgccggcg acgaccgagc tgccgacggt cagcttgtcg
      961 ggggcgagaa cgtagcgctt ctcgccgtcg acgtaatgaa ggagcgcgat gttggcgctg
     1021 cggttagggt cgtactcgat gctggcgacc ttggcgggc
//
LOCUS       DAOQYE010000950         1059 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_191365_length_1059_cov_2.495020, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000950 DAOQYE010000000
VERSION     DAOQYE010000950.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_191365_length_1059_cov_2.495020"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /gene="folK"
                     /locus_tag="PKN52_12495"
     CDS             complement(<1..358)
                     /gene="folK"
                     /locus_tag="PKN52_12495"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HNR00819.1"
                     /translation="MSASDGSRSASSERATALAAKAAPPVAYVALGANLGDPAAQFAR
                     AAHALADLVPVTGRSRLYEGPAVGGPSGQPPFLNAVIAVDGSAFVGREEELLAELLRV
                     ELDLGRVRRERWGPRVI"
     gene            complement(355..>1059)
                     /gene="folP"
                     /locus_tag="PKN52_12500"
     CDS             complement(355..>1059)
                     /gene="folP"
                     /locus_tag="PKN52_12500"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HNR00820.1"
                     /translation="LRMREEGALVLDVGGESTRPGAAPVGLGEEARRVLPVIEALAAA
                     GAVVSVDTRKAEVAAAALAAGAAVVNDVGGLRDPAMLAACAAAGAPAIVMRMQGEPRT
                     MQAAPRYGDVVAEVEAFLLEQARRAAAAGVPSVVLDPGIGFGKDLDHNLDLLRATARL
                     AGHGHPLLVGASRKAFIGRLAGGVGPESRLSGTLAAHLAAAAAGAALLRVHDVAAHAQ
                     ALAVATALATGQAEPG"
BASE COUNT          141 a          402 c          403 g          113 t
ORIGIN      
        1 cgatgacgcg tggaccccag cgttcgcgcc gcacgcgccc gaggtccagc tccaccctca
       61 ggagctcggc gaggagctcc tcttccctgc ctacgaacgc tgacccgtcg acggcgatca
      121 cggcgttcag gaacggaggc tggcccgagg ggccgccgac ggcgggcccc tcgtacagtc
      181 gcgagcgacc cgtcaccggg accaggtcgg cgagcgcgtg ggcggcgcgg gcgaactggg
      241 cggccgggtc gccgaggttg gcgccgagcg ccacgtaagc caccggtgga gccgccttcg
      301 cggcgagagc cgtcgcccgc tccgacgagg cggagcggct accgtcggac gcgctcaccc
      361 cggctccgcc tggccggtcg cgagggcggt cgccaccgcg agggcctgag cgtgcgcggc
      421 gacgtcgtgg acgcgcagca acgccgcccc cgcggcggcg gcggcgagat gagcggccag
      481 ggttcctgac aagcgcgact cgggtccgac accgcccgcg agcctcccga tgaaggcctt
      541 gcgcgacgcg ccgacgagga gggggtgacc gtgaccggcg aggcgcgcgg tggcgcgcag
      601 caggtcaagg ttgtggtcca ggtccttgcc gaagccgatg cccgggtcaa gcacgaccga
      661 cggaacgccg gcggcggcgg cgcggcgtgc ctgctctagg aggaacgcct cgacctcggc
      721 cacgacgtcg ccgtaccgcg gcgcggcctg catggtgcga ggctcgccct gcatgcgcat
      781 gacgatggcg ggcgcgccgg ccgcggcgca agcagcgagc atggcggggt ccctcaagcc
      841 acctacgtcg ttgacgacgg ccgcgccggc ggccaaggcg gccgcggcca cctcggcctt
      901 gcgcgtatcg acgctgacga ccgcgccggc ggccgcgagc gcctcgatca ccggtagcac
      961 gcgccgcgcc tcctcgccca ggcctaccgg cgccgcgccc ggccgcgtcg actcgccgcc
     1021 gacgtcgagg accagtgcgc cttcctcgcg catgcgcag
//
LOCUS       DAOQYE010000951         1058 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_191677_length_1058_cov_2.632104, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000951 DAOQYE010000000
VERSION     DAOQYE010000951.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_191677_length_1058_cov_2.632104"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1058
                     /locus_tag="PKN52_12505"
     CDS             <1..>1058
                     /locus_tag="PKN52_12505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HNR00821.1"
                     /translation="FSIKVLLESLVRNEDGFLVTRADIERLAAYDAKKRYEDEIPFMP
                     ARVILQDFTGVPAVVDLAALRSAMRRMGGDPDKINPQVPVDLVIDHSVQVDEYDDPLA
                     LLHNSQIEFQRNMERYEFLRWGQAAFANFNVVPPASGIVHQVNLEYLARGVLSKPLGD
                     KEVVFPDSLVGTDSHTTMINGLGVVGWGVGGIEAEAVMLGQPYYMVIPEVVGFRLKEK
                     LPEGSTATDLVLVVTQMLRKHGVVEKFVEFFGDGLASLSLPDRATIANMAPEYGATMG
                     FFPVDDVALDYLRLTSRPKELLDLVERYCKENMLFWSPGAKDPEFTSVLELDLGSVQP
                     SLAGPKRPQDRVPLRDMK"
BASE COUNT          178 a          381 c          326 g          173 t
ORIGIN      
        1 gttctccatc aaggtcctgc tcgagtcgct cgtgcgcaac gaggacggct tcctcgtgac
       61 gcgggccgat atcgagcggc tcgccgccta cgacgccaag aagcgctacg aggacgagat
      121 cccgttcatg ccggcgcgcg tgatcctgca agacttcacg ggcgtgccgg ccgtcgtcga
      181 cctggccgcg ctgcgcagcg ccatgcggcg catgggcggc gaccccgaca agatcaaccc
      241 gcaggtcccc gtcgacctcg tcatcgacca ctccgtccag gtagacgagt acgacgaccc
      301 gctcgcgctc ctccacaact cccagatcga gttccagcgc aacatggagc gttacgagtt
      361 cctccgctgg ggccaggcgg cgttcgccaa cttcaacgtc gtcccgccgg cctccggcat
      421 cgtccaccag gtcaacctcg agtacctggc ccgcggcgtc ctctccaagc cgctcggcga
      481 caaggaggtc gtgttccccg actcgctcgt cggcaccgac agccacacga ccatgatcaa
      541 cggcctgggc gtcgtcggct ggggcgtcgg cggcatcgag gccgaggccg tcatgctcgg
      601 ccagccgtat tacatggtca tcccggaagt cgtcggcttc cgcctcaagg agaagctgcc
      661 agagggctcg acggccaccg acctcgtgct cgtcgtcacc cagatgctcc gcaagcacgg
      721 cgtggtagag aagttcgtcg agttcttcgg cgacgggctg gcctcgctgt cgctgcccga
      781 ccgcgcgacg atcgcgaaca tggccccgga gtacggcgcg acgatggggt tcttccccgt
      841 cgacgatgtg gccctggact acctgcgcct cacgagccgc cccaaggaac tccttgatct
      901 tgtcgagcgc tactgcaaag agaacatgct cttttggtcg ccaggcgcga aggaccccga
      961 gttcacctcg gtcctggaac tggacctggg atcggtgcag ccgtcgctgg ccggccccaa
     1021 gcgcccacag gaccgcgtcc cgctgcgcga catgaaga
//
LOCUS       DAOQYE010000952         1058 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_191701_length_1058_cov_2.450648, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000952 DAOQYE010000000
VERSION     DAOQYE010000952.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_191701_length_1058_cov_2.450648"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="PKN52_12510"
     CDS             <1..214
                     /locus_tag="PKN52_12510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020886703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HNR00822.1"
                     /translation="ELLRELVDSQEPLVITQNGEAKAVVLDVATYDQLLETSALLKLL
                     SLADRQAEEGMLEAAADAIANYRLEG"
     gene            291..608
                     /locus_tag="PKN52_12515"
     CDS             291..608
                     /locus_tag="PKN52_12515"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNR00823.1"
                     /translation="MLLTDGAARDLDEWYAAAYARGGAGEAARILDRIEVVMQALADG
                     SMREEAVPELRQLGRHAERQVVTSEGLRVVFRPGAERVVVVLLAPVKRSFQALLERRL
                     LDA"
     gene            complement(735..>1058)
                     /gene="ssrA"
                     /locus_tag="PKN52_12520"
     tmRNA           complement(735..>1058)
                     /gene="ssrA"
                     /locus_tag="PKN52_12520"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT          167 a          348 c          388 g          155 t
ORIGIN      
        1 cgagctgctg cgcgagctcg tcgacagcca ggagccgctg gtcatcacgc agaacggcga
       61 ggccaaggcc gtcgtcctgg acgtggcgac ctacgaccaa ctgctggaga cgtcggccct
      121 cctcaagctc ctgtcgttgg ccgatcggca ggccgaggag ggcatgctcg aggcggccgc
      181 ggacgctatc gccaactaca ggctggaagg gtgagggctc cggtgcccct ccggcggcgg
      241 gcacggcgcc gtgcgcgccg cgctggctcg cgcggcgcgc tatgaggccc gtgctgctca
      301 ccgacggcgc cgctcgcgac ctggacgagt ggtacgcggc ggcctacgcc cggggcggcg
      361 cgggcgaggc ggcccgaata ctggaccgga tagaggtcgt catgcaggcg ctggccgacg
      421 gctccatgcg cgaggaggcc gtccccgaac tccgccaact gggcaggcat gccgaacgtc
      481 aggtcgtcac gagcgagggc ttgcgcgtgg tcttccggcc aggagcggag cgggtcgtgg
      541 tcgtcctgct ggcgccggtg aagcgctctt tccaggcgct ccttgagcgg cggctgctcg
      601 acgcctgagg gcgggccgca ggcaagaccg cgcagccgcc tggcgccgca gcggcacccg
      661 cgcgacggat gaagcatgaa gacgcatgaa gcatgaagaa gcccgcggcc ggatgaggac
      721 cgcgggcttc cgcttggtgg aggcggcggg agtcgaaccc gcgtccgaaa ccccctcagg
      781 tgggcgtcta cgcgtgtagc cgaggatcgg ttgtcggcgc caggctggct cttcggccgg
      841 cgctgcctag cgcttagacg tcataagtct cgccttgaag gtcaacgtcc gaaccccaag
      901 accagcccac tttaggacgt tcgatccggc gagccatggg cggggcttcc ggggaacgct
      961 aactgcgatt aagcagccag agcgtagtta ttgttgccag ttaatggcgt ggcagcttgt
     1021 tgacgcggcc agctgcctcc gcgacgcgca acctcacc
//
LOCUS       DAOQYE010000953         1056 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_192248_length_1056_cov_3.566434, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000953 DAOQYE010000000
VERSION     DAOQYE010000953.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_192248_length_1056_cov_3.566434"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /gene="upp"
                     /locus_tag="PKN52_12525"
     CDS             complement(<1..390)
                     /gene="upp"
                     /locus_tag="PKN52_12525"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HNR00824.1"
                     /translation="MAVTVVDHPLVQHKLSILRDKETSVREFRALCQELTMLMAFEAM
                     RDLELEDATVETPVALAHVKRLAGKKLALIGILRAGLVMVDGILALMPTARVGHIGLY
                     RDPDTLKPVQYYSKLPSDVGERDVFLLD"
     gene            complement(518..608)
                     /locus_tag="PKN52_12530"
     tRNA            complement(518..608)
                     /locus_tag="PKN52_12530"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(572..574),aa:Ser,seq:cga)
     gene            749..822
                     /locus_tag="PKN52_12535"
     tRNA            749..822
                     /locus_tag="PKN52_12535"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:781..783,aa:Gln,seq:ctg)
     gene            884..957
                     /locus_tag="PKN52_12540"
     tRNA            884..957
                     /locus_tag="PKN52_12540"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:916..918,aa:Gln,seq:ttg)
BASE COUNT          166 a          337 c          359 g          194 t
ORIGIN      
        1 gtcgaggagg aagacatcgc gctcgccgac gtcacttggc agcttggagt agtactgaac
       61 gggcttgagg gtgtctgggt cgcggtagag gccgatatgg ccgacgcggg ccgtcggcat
      121 cagggcgagg atgccgtcga ccatgacgag cccggcgcgc aggatgccga tgagcgcgag
      181 cttcttgccg gccagacgct tgacgtgggc aagcgcgacc ggcgtctcga ccgtcgcgtc
      241 ttccagctcg aggtcgcgca tggcctcgaa cgccatcagc atggtcagtt cctggcatag
      301 cgccctgaac tcccttacgc tcgtctcctt gtcgcggagg atggagagct tgtgctggac
      361 gagggggtgg tcgactaccg tgacggccat cttggtgttc ctttcgcgac cgcgcgccgc
      421 atccgcctgg tcggcgcatg ggcggcgggg agctgcggtc tgatccggcg gcccgggacg
      481 ttcaacagaa cggccgccct atagggcggc cgtggcgtgg cggagagggt gggattcgaa
      541 cccacggtgc agcgtagcca cacaacggtt ttcgagaccg tcccattcaa ccactctggc
      601 acctctccat cgcgcggccg gcgtagcgcg tcgccccgct tgccgagctt gcgaagtata
      661 tcgtacccgc ttgggtggtg tgcgcgccgc tgcttgacgc atacggccgc gtctcataca
      721 ctcccagatg cgcctcgggc cgtagcgttg gcccatcgtc taatggcagg acaacggatt
      781 ctggttccgt cggtctaggt tcgaatccta gtgggccagc caaccgccgc tcggggcagc
      841 acccttggaa agcgccagcc aagcgcgttc acgaactcgg cgatggccca tcgtctaatg
      901 gcaggacacc cggttttggt ccggtcggtc ggagttcgaa tctctgtggg ccagccaaga
      961 cgcctccctc gcgggaggcg ttctcatagg ccggtctggc gggcgtcagc cgccgtactc
     1021 gtagaagccg cgccccgact tacgcccgag atgacc
//
LOCUS       DAOQYE010000954         1056 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_192436_length_1056_cov_1.981019, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000954 DAOQYE010000000
VERSION     DAOQYE010000954.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_192436_length_1056_cov_1.981019"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            497..>1056
                     /locus_tag="PKN52_12545"
     CDS             497..>1056
                     /locus_tag="PKN52_12545"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNR00825.1"
                     /translation="MSEAGVNRVVEQARDALATFVKNAPEGTKLPPERQLAQRLGVSR
                     TTLRDGVSRLAQLGLLEVRHGDGTYVRSPGASQLVPLLRPLVEGRHTTLEELFELKRR
                     VEPAVAAMAAGNCTDAQATAMRAAIQRERDEFNGAALPRRTSRKSPQRRQVHELIADA
                     AGESLLVALVSLANELVAPYLAKGLSP"
BASE COUNT          170 a          382 c          350 g          154 t
ORIGIN      
        1 ccgggtccgc agacccaccg tacgttcgcc ggcgctcggt gccccgtgga gcgtgccggg
       61 ctgacgcgtg ccgggccaag gcagcgcgac tactagtccg gtccccaagc tccgttagcc
      121 agccaagacc tggcggtcga tggacccgcc gtagtcagaa tctaggagtc ggactcctat
      181 gccgtcctgc aaacacccgg tgcgaccgca accctttcag gaggttacgc gtcggtaacc
      241 gcgaccctga cgacaacgag ccgcgacgag agccgcgacg agagccgcga cggtgaattt
      301 gataactcgc agatggtccg acctcgttgc tattggttgg accgctgccg acattggtcg
      361 gaccatctcc ttataggtcc gaccacttct gctacaatcc gtcagggtca cgcaacttgg
      421 cccgttcggt ccgccgccgc tcctcggggc tcccggccga cacagggcca taccgggccg
      481 cgcaggagtt cagacggtga gcgaggcagg cgtcaatcgg gtcgtggagc aagcgcgcga
      541 tgcgctggcg acgttcgtga agaacgcccc cgagggcacg aagctgccgc cagagcgcca
      601 gctcgcccag cgcctcggcg tctcacgcac cacgctccgc gacggcgtca gccgcctggc
      661 gcagctcggc ctgctcgagg tgcggcatgg cgacggcacc tacgtgcgct ctccgggtgc
      721 ctcccagctc gtgccgctcc tgcggcccct ggtcgagggt cggcacacca cgctggaaga
      781 gctgttcgag ctcaagcgcc gcgtggagcc ggcggtggcg gccatggccg ctggcaactg
      841 cacggacgcg caggcgaccg ccatgcgcgc cgccatccag cgcgaacgcg acgagttcaa
      901 cggcgccgcc ctaccgcggc ggacgagccg taagtcgccg cagaggcgtc aggtgcacga
      961 gttgatcgcg gacgccgcgg gcgaatcgct gctcgtggcg ctcgtcagcc tcgccaacga
     1021 gctcgtcgcc ccctacctgg cgaagggcct ctcccc
//
LOCUS       DAOQYE010000955         1056 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_192464_length_1056_cov_1.835165, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000955 DAOQYE010000000
VERSION     DAOQYE010000955.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_192464_length_1056_cov_1.835165"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..742
                     /locus_tag="PKN52_12550"
     CDS             <1..742
                     /locus_tag="PKN52_12550"
                     /inference="COORDINATES: protein motif:HMM:NF037429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VLRF1 family aeRF1-type release factor"
                     /protein_id="HNR00826.1"
                     /translation="FEAFLGQIDEVADYSRSVDTEAWSDFRSSRRNPGAAANAAARGG
                     ADVDRFRRRLEEASARLYRTLAPEVEELMAAGRADRVILIGQPQTLGAFESAMSGGMA
                     AKVVGRLAPPANPAAPAREWLPLVADLIAQTEAAREEELLDRVREAGVWGFEQTLGLL
                     QERKLRTLVVPFGQARSVYVTESGWVAATPGEAEALRPGETVAEASLLEVLPDLVRGT
                     GTALEFVEGTAEERLVNEFGGLAGMMGK"
     gene            830..>1056
                     /locus_tag="PKN52_12555"
     CDS             830..>1056
                     /locus_tag="PKN52_12555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HNR00827.1"
                     /translation="METTKNPEGYRMKNPAHPALLVRDELLAQYGLSVTESADILGVT
                     RQALSTFLNERSSLSSEMALRIENVDNASSAT"
BASE COUNT          166 a          338 c          392 g          160 t
ORIGIN      
        1 cttcgaggcg ttcctcggcc agatcgacga ggtcgccgat tacagccgct ccgtcgacac
       61 ggaggcctgg tccgacttcc gttcctcccg gcgcaaccca ggcgccgcgg ccaacgcggc
      121 ggcgcggggc ggggcggacg tcgaccgctt caggcgccgc ctggaggaag ccagcgcgcg
      181 cctctaccgg acgctggcgc ccgaggtcga ggagctcatg gcggcggggc gggcggaccg
      241 cgtcatcctg atcgggcagc cgcagacgct cggtgcgttc gagagcgcca tgagcggagg
      301 catggccgcc aaggtggtgg gcaggctggc gccgccggcc aaccccgccg cgcccgctcg
      361 cgagtggctg ccgctcgtgg cggacctgat cgcgcagacc gaggccgcgc gcgaggagga
      421 gctgcttgac cgggtgcgcg aggccggcgt ctggggcttc gagcagaccc tcggtcttct
      481 gcaggagcgc aagctccgca ccctggtcgt cccgttcgga caggcgcgca gcgtgtacgt
      541 gaccgagagc ggctgggtgg cggccacccc gggcgaggcc gaagcgctgc ggcccggcga
      601 gacggtcgcg gaggcgagcc tcctcgaggt gctgccggac ctggtgcgcg gcaccggcac
      661 ggcgctcgag ttcgtcgaag gcactgccga ggaacggctg gtcaacgagt tcggcgggct
      721 ggccgggatg atggggaagt aggcgtcgtc cgtcacgtga cgcttgacgg catgttgggg
      781 cggcgctagt atgcgcgcac atggggttgc acttcgagga ctatcactga tggagacgac
      841 caagaacccc gaaggttacc gtatgaagaa cccagctcat cctgctctgt tggtaaggga
      901 tgaactactc gctcaatacg ggctctcggt tacggagagc gccgatatcc taggtgtcac
      961 gcgccaggcg ttgtcgacgt tcttgaacga acggtcctcc ttatcatctg agatggcgct
     1021 gaggatcgag aacgtcgaca acgcctcgtc ggccac
//
LOCUS       DAOQYE010000956         1055 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_192614_length_1055_cov_3.024000, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000956 DAOQYE010000000
VERSION     DAOQYE010000956.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_192614_length_1055_cov_3.024000"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1055
                     /locus_tag="PKN52_12560"
     CDS             <1..>1055
                     /locus_tag="PKN52_12560"
                     /inference="COORDINATES: protein motif:HMM:NF019924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HNR00828.1"
                     /translation="PKALARLGHEVLVVTPWYADLGGAVRPYWVGDVQAPFDRGFEPV
                     GVGVLEGGVLEDGVLEDGAPGFGAVSQGAPSGGSVRYAFVGHQDYRRAKLYGYPDDAR
                     RFARFSRAVPSVAQRLGFVPDLLHAHDWHTGYLPMVLARGWHLPPGFPALRSVFTIHN
                     VQYQGESSLEDAVRWLRLGPDVMGSYLDHFGRANAMQAGVGFATRVTTVSPTYAEEIQ
                     TPEYGYSLDGTFRSLSGELTGILNGIDTDVWDPASDTALPKPYSSTDLAGKQAAKAEL
                     ARRFGIGAERPLLAVVSRLAEQKGMDLLAGAAPALLASGWSLVVLGAGDQALEAALKA
                     LARAHPGLVGVRIGYDE"
BASE COUNT          132 a          374 c          396 g          153 t
ORIGIN      
        1 gcccaaggcg ctggcgcgcc ttggccacga ggtcctcgtc gtaacgccct ggtacgccga
       61 cctcggcggg gccgtgaggc cctactgggt cggcgacgtg caggcgccgt tcgaccgcgg
      121 cttcgagccc gtcggcgtcg gggtgttgga gggcggggtg ttggaggacg gggtgttgga
      181 ggatggcgcg ccgggcttcg gcgcggtgag tcagggcgcg ccgagcggcg gcagcgtgcg
      241 ctacgccttc gtcggccacc aggactaccg ccgggcgaag ctctacggct acccggacga
      301 cgcgcggcgg ttcgcgcgct tctcgcgggc cgttcccagc gtcgcgcagc ggctcgggtt
      361 cgtgccagac ctcctgcacg cgcacgactg gcacacgggc tacctgccga tggtactggc
      421 ccgcggttgg cacctgccgc cgggcttccc cgccctacgc agcgtgttca ccatccacaa
      481 cgtccagtac cagggcgagt cgagcctgga ggacgccgtg cgctggctgc gcctcggtcc
      541 cgacgtcatg ggctcgtacc ttgaccactt cgggcgcgcc aacgccatgc aggcgggggt
      601 cggcttcgcg acgcgcgtga cgaccgtgag cccgacctac gccgaggaga tccagacccc
      661 cgagtacggt tactcgctcg acggcacatt ccgctcgctc tcgggcgagc tgacggggat
      721 cctgaacggc atcgacacgg acgtctggga cccggccagc gacacggcgc tgccgaagcc
      781 gtattcgtcg accgacctcg ccggcaagca ggcggccaag gcggagctgg cgcggcggtt
      841 cggcatcggg gccgagcgcc cgctcctcgc cgtggtgtcg cgcctcgccg agcagaaggg
      901 catggacctg ctcgccggcg cggcgccggc gctcctagct agcggctggt cgctcgtcgt
      961 gctcggcgcc ggcgaccagg ccctcgaggc ggcgttgaag gcgctggcgc gagcgcaccc
     1021 tggcctggtc ggcgtgcgca tcggctacga cgagg
//
LOCUS       DAOQYE010000957         1055 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_192716_length_1055_cov_2.264000, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000957 DAOQYE010000000
VERSION     DAOQYE010000957.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_192716_length_1055_cov_2.264000"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..856)
                     /locus_tag="PKN52_12565"
     CDS             complement(<1..856)
                     /locus_tag="PKN52_12565"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNR00829.1"
                     /translation="MRATATEIVRLTGVGKTFARPGGQHLAVLEGVSLTLQEGEIVGI
                     VGRSGSGKSTLLRVIAGLTEPSTGSATYRGAPIAGPPPGVAMVFQSFALLPWLTVLGN
                     VELGLQALGASREETRERAMEAIDLIGLDGFEQAYPRELSGGMRQRVGIARALVVHPD
                     VLLMDEPFSALDVLTAEALRAEILELWSSGRLPTSVIVLVTHNIEEAVLMCDRVLVFA
                     SRPGRVAAEVAVDLPQPRERTDPRFGRLVERLYSELAGLTVPITPAQAGPAPPDVFRH
                     LPHASPGVM"
     gene            complement(861..>1055)
                     /locus_tag="PKN52_12570"
     CDS             complement(861..>1055)
                     /locus_tag="PKN52_12570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00830.1"
                     /translation="ELVHWGDETIEAAGLGAYIVKATAAGDGGRIVLGIAVMSLYVVL
                     INRLVWEPLQRYVSRRFRLE"
BASE COUNT          160 a          365 c          380 g          150 t
ORIGIN      
        1 ccatcacgcc gggcgacgca tgggggaggt ggcggaagac gtccggtggc gcggggcccg
       61 cctgcgccgg cgttatcggc acggtgagtc cggccagttc ggagtagaga cgctcgacga
      121 gcctgccgaa gcgcggatcg gtccgttcgc gtggctgggg caggtcgacg gcgacctcgg
      181 ccgccacccg cccgggtcga ctcgcgaaga cgaggacgcg gtcgcacatg aggacagcct
      241 cctcgatgtt gtgggtgacg agcacgatga cggaggtcgg cagccggccg ctcgaccaca
      301 gctccaggat ctcggcccgg agcgcttcgg cggtgagcac gtcgagggcc gagaacggct
      361 cgtccatgag gagcacgtcc gggtgtacga cgagcgcacg cgctatcccg acgcgttggc
      421 gcatgccacc agatagctca cgcgggtagg cctgctcgaa gccgtcgagc ccgatgaggt
      481 cgatggcttc catggcccgc tcgcgcgtct cttccctgct cgcgccgagc gcttggagcc
      541 cgagttccac gttgccgagc accgtcaacc acgggagcag ggcgaagctc tggaagacca
      601 tggccacccc cggcggcgga cccgcgatcg gcgcgccgcg gtaggtggcg gagccggtcg
      661 acggttcggt caggccggcg atgaccctga ggagcgtgct cttgcccgag cccgaccgcc
      721 ccacgatgcc gacgatctcg ccttcctgca gggtcagcga cacgccctcg aggaccgcga
      781 ggtgttgccc gccgggccgg gcgaaggtct tgccgacccc ggtgagccgc actatctcgg
      841 tggccgtcgc cctcatcccc tcactctagg cggaagcgcc tgctcacgta ccgttggagg
      901 ggctcccaga cgaggcggtt gatgaggacg acgtagaggc tcatgacggc gatgcccagc
      961 acgatgcgcc cgccgtcgcc cgccgcggtc gccttcacga tgtaggcgcc aaggccggcg
     1021 gcctcgatgg tctcgtcgcc ccaatggacg agctc
//
LOCUS       DAOQYE010000958         1054 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_192937_length_1054_cov_3.045045, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000958 DAOQYE010000000
VERSION     DAOQYE010000958.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_192937_length_1054_cov_3.045045"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(177..506)
                     /gene="rplX"
                     /locus_tag="PKN52_12575"
     CDS             complement(177..506)
                     /gene="rplX"
                     /locus_tag="PKN52_12575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HNR00831.1"
                     /translation="MSRVKTRIKKGDNVKVIAGKHKGSSGEVISVDREAGRVLVKNVN
                     IIRKAQRPTQENPRGGFVEQEASLHLSNVQLLDPKSGAPTRIAYRLQPDGTKVRVSVK
                     SGTQLDE"
     gene            complement(507..875)
                     /gene="rplN"
                     /locus_tag="PKN52_12580"
     CDS             complement(507..875)
                     /gene="rplN"
                     /locus_tag="PKN52_12580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HNR00832.1"
                     /translation="MIQQETFLDVADNSGARTIQCIRVLGTGQQWVGSVGDVIVAAVK
                     DAIPHAAVKKGDVVKAVIVRTKKEVNRKDGSSIRFDTNAAVILNAQNEPRGTRVFGPV
                     ARELREKKFMRIVSLAPEVL"
     gene            complement(892..>1054)
                     /locus_tag="PKN52_12585"
     CDS             complement(892..>1054)
                     /locus_tag="PKN52_12585"
                     /inference="COORDINATES: protein motif:HMM:NF012586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HNR00833.1"
                     /translation="PLYGKVVTVSKRYLAHDEKNAYQVGDLVEIAASRPISRRKRFVV
                     ARLIEKARA"
BASE COUNT          143 a          334 c          359 g          218 t
ORIGIN      
        1 ggaggccttg tcgatgacct tggagtcctc gccggcctcg ccgacgccca tgttgacgac
       61 gatcttctcg atgcgcggaa cggccatggg gttcgtgtag ccgtggcgct cctcgagctg
      121 ggggcggatg gacgcgtagc gcttcttgat gggtagttct atcttctcca ttgccctcac
      181 tcgtccagct gcgtgccgga cttgacggac acgcgcacct tcgtgccgtc cggctgcaga
      241 cggtacgcga tgcgggtagg ggcgccgctc ttcggatcga gcagctgcac gttgctcagg
      301 tggagcgacg cctcctgctc gacgaagccg ccgcgcgggt tctcctgcgt ggggcgctgc
      361 gccttccgga tgatgttgac gttcttcacg aggacgcgac cggcctcgcg gtcgaccgag
      421 atcacctcgc cgctggagcc cttgtgcttg ccggcgatga ccttgacgtt atcgcccttc
      481 ttgatgcgcg tcttgacgcg tgacattcac agcacctccg gggccaggga cacgatgcgc
      541 atgaacttct tctcgcgcag ctcgcgcgcg acgggtccga agacgcgggt gccgcggggc
      601 tcgttctgag cgttgaggat gacggcggcg ttcgtgtcga agcggatcga ggagccgtcc
      661 ttgcggttga cttccttctt cgtgcgcacg atgacggcct tgacgacgtc acccttcttg
      721 acggcggcgt gcgggatggc atccttgacg gccgcgacga tcacgtcgcc tacggagccg
      781 acccactgct ggcccgtgcc aagcacgcgg atgcactgga tggtgcgggc gccggagttg
      841 tcggcgacgt cgaggaaggt ttcctgttgg atcatgtcag ctcactcccg cttacgcgcg
      901 cgccttctcg atcaagcgcg ccaccacgaa gcgcttgcgg cggctgatgg ggcggctggc
      961 cgcgatctct actaggtcgc ccacctggta cgcgttcttc tcgtcgtgcg ccaggtagcg
     1021 cttggagacg gtgacgacct tgccgtagag gggg
//
LOCUS       DAOQYE010000959         1054 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_192966_length_1054_cov_2.676677, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000959 DAOQYE010000000
VERSION     DAOQYE010000959.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_192966_length_1054_cov_2.676677"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /locus_tag="PKN52_12590"
     CDS             complement(<1..608)
                     /locus_tag="PKN52_12590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtpA/SgcQ family protein"
                     /protein_id="HNR00834.1"
                     /translation="MSWLESVFGVGRPIIGMCHLPALPGDPGFDAENGMRGIMTRVES
                     DLAALQEGGVDAVLFSNEFSLPYLTKVHPVTTAAMARVIGELLPGIRVPFGVNVLWDA
                     KASLDLAVATGASFVREIFSGVYASDFGLWNTNVGEFVRHQHAIGGAHVRLLYNIVPE
                     AAQYLANRDFAQIVRTTVFNCHPDALCVSGLTAGSATSTAALR"
     gene            complement(655..>1054)
                     /locus_tag="PKN52_12595"
     CDS             complement(655..>1054)
                     /locus_tag="PKN52_12595"
                     /inference="COORDINATES: protein motif:HMM:NF012669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HNR00835.1"
                     /translation="NDLFIAGQIAFDPSITVIVTGGTLREGFNTLVGPLTETFLRNVR
                     VDRTVLSADAVDPSFGVSNATFAESSIKQLMVEAATHVTLVADASKFGKRALARVCAL
                     DEIDVVVTDDALSDANQKALSHATTKLVLT"
BASE COUNT          199 a          334 c          344 g          177 t
ORIGIN      
        1 cgtagagcgg ccgtcgaagt ggcggagccg gcggtcagcc cggagacgca gagtgcatcc
       61 ggatggcagt tgaagacggt cgtgcgaacg atctgggcga agtcgcggtt ggccaggtac
      121 tgggccgcct ccgggacgat gttatagagc aagcgcacgt gggcgccgcc gatggcgtgc
      181 tgatgcctga cgaactcccc gacgttcgtg ttccagagcc cgaagtcgct cgcgtagacg
      241 ccgctgaaga tctcccgcac gaacgaggcc cccgtcgcca cggcgagatc gagcgatgcc
      301 ttggcgtccc acaggacgtt cacgccgaat ggcacgcgga taccgggcag gagttcacct
      361 atcacgcgcg ccatggccgc cgtggtgacg gggtgaacct tggttaggta aggcaggctg
      421 aactcgttgg agaagaggac cgcgtcaacg ccaccttcct gcagggcggc gagatcgctc
      481 tcgaccctcg tcatgatgcc gcgcatgcca ttctcggcat cgaagcccgg atccccgggc
      541 aaggcaggca agtgacacat gccgatgatc ggtcttccga ctccgaacac ggactccagc
      601 caactcatcc gactcctcct tcacgggccg tggggctcgc cggcctagcg cgcgtcatgt
      661 aagtaccaac ttcgtcgtcg catgcgatag cgccttctgg ttcgcgtcgc tcaaggcatc
      721 gtcggtgacc acgacatcga tctcgtcgag cgcgcagact ctggccaacg cacgcttccc
      781 gaacttcgag gcgtcggcca cgagcgtcac gtgagtggca gcctcgacca tcagctgctt
      841 gatggaggac tcggcaaagg tggcgttgga gacaccgaag gaggggtcga ccgcgtcggc
      901 cgacaacacc gttcggtcca cgcggacgtt acgcaagaac gtctccgtga ggggacccac
      961 caaggtgttg aagccctccc ggagcgtccc tccggtgacg atcacggtga tgctcggatc
     1021 gaaggctatc tgtccggcga tgaacaggtc gttc
//
LOCUS       DAOQYE010000960         1054 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_192988_length_1054_cov_2.526527, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000960 DAOQYE010000000
VERSION     DAOQYE010000960.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_192988_length_1054_cov_2.526527"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1054)
                     /locus_tag="PKN52_12600"
     CDS             complement(<1..>1054)
                     /locus_tag="PKN52_12600"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HNR00836.1"
                     /translation="GSAALLGVWHVPFTADLVGTLEVRALEPGRAGATLELGLSVPGA
                     TWCAVDLASAPGEHYLGFGERFDSIDKRGREVRVEVVNGASGNLAYKPVPFFVSSRGY
                     GVHVVTDARLDARMAVPDSPATASLRVANPSLRLRFYRGTPAEVLAAYTSEAGRPALP
                     PAWVFGPWKSRDWTLEDQSTVLEDVRESRRHDLANSVKLIDAKWETAEHTFVFDRVKY
                     PDAAAMVREVRESGMRLVLWLSPWLVLNDDTAEVHAAAAAKGYLIKNGRGDTYVHRLG
                     NSPDFVGSCVDFTNPAAVTWWQENVRKLVELGVDGFKTDFGEQVPEDAVFFDGSTGAQ
                     MHNVFPRLYNAVTYEA"
BASE COUNT          157 a          362 c          377 g          158 t
ORIGIN      
        1 atggcctcgt aggtcacggc gttgtagagc cgggggaaga cgttatgcat ctgcgcgccg
       61 gtgctgccgt cgaagaagac ggcgtcctcg ggcacctgct cgccgaagtc cgtcttgaag
      121 ccgtccacgc cgagctcgac gagcttgcgc acgttctcct gccaccaggt cacggcggcg
      181 gggttcgtga agtcgacgca gctccccacg aagtcggggc tgttgccgag gcggtggacg
      241 tacgtatcgc cgcgcccgtt cttgatgagg tagcccttgg cggccgcggc cgcgtggacc
      301 tcggccgtgt cgtcgttcaa cacgagccac ggcgagagcc acaggaccag gcgcatgcct
      361 gactcgcgga cctcgcgcac catggcagcc gcgtccgggt acttgactct gtcgaagacg
      421 aacgtgtgct ctgcggtctc ccacttggcg tctatgagct tgacgctgtt agccaggtcg
      481 tgccggcgcg actcgcgcac gtcctccagg accgtggact ggtcctcgag cgtccagtcg
      541 cgggacttcc atgggccgaa cacccaggcg ggcggcaggg ccggccggcc ggcctcggac
      601 gtgtaggcgg cgaggacctc cgccggcgtg ccccggtaga accgcaagcg cagcgagggg
      661 ttcgcaacgc gcagcgaggc ggtggccggc gagtcgggga cggccatgcg cgcatcgagg
      721 cgcgcgtcgg tcacgacgtg gacgccgtag ccgcggctcg acacgaagaa cgggacgggc
      781 ttgtacgcca ggttgccgga ggcgccgttc acgacctcta cccgcacctc acggccgcgc
      841 ttgtcgatgc tgtcgaaacg ctcgccgaag ccgaggtagt gctcccccgg cgccgatgcg
      901 aggtcgacgg cgcaccaggt ggcgcctggg acgctaagcc cgagttccag tgtcgccccg
      961 gcccttccgg gctcgagcgc gcgcacctcg agcgtgccga ccaggtctgc cgtgaatggc
     1021 acgtgccaga ccccgaggag cgccgccgag cccg
//
LOCUS       DAOQYE010000961         1054 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_193030_length_1054_cov_2.269269, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000961 DAOQYE010000000
VERSION     DAOQYE010000961.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_193030_length_1054_cov_2.269269"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(132..>1054)
                     /locus_tag="PKN52_12605"
     CDS             complement(132..>1054)
                     /locus_tag="PKN52_12605"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNR00837.1"
                     /translation="LGAWASEIAPFFHRPNSRREMLMALSFLAPSLLVLIAFVYFPIL
                     WAFGISFTNWQAGSATTSFVGAKNYVDTLTSSDFHNALKNSLYFMVLKLPIDMVLSLL
                     VALLLNQKLRGMSFFRVALFMPVVTSMVAAAAIWRVLYNPSSGFLNTVLNSLGLPSQR
                     WLRDPSLAMPSVVLVALWKGLGYNIVIFLAGLQGIGRVYYEAAEIDGATIWQRFRYIT
                     LPLLSPVTYFVLLIGVINSFKVFSLIHVLAPDGGPLNSVEVLVFYLYRLTFQRFKFGQ
                     GAAVSFILFAIVLGLTFIQQRVAEKRVHYE"
BASE COUNT          229 a          302 c          369 g          154 t
ORIGIN      
        1 gaggatctcc tgcggggact tcagggccgt gccgaccatc cacacgaacg ggaacgcggt
       61 ggccgcgctc atgacgatga ggaggacgta cgtgcccgag cgaccgaaga acctgccgag
      121 ccggtaaccg ttcattcgta atgcacccgc ttctcagcca cgcgctgttg gatgaacgtg
      181 agacccaaga cgatcgcgaa gaggatgaac gacaccgccg ccccctgccc gaacttgaag
      241 cgctgaaacg tgagcctgta gaggtagaag acgagcacct ccacgctgtt caggggcccg
      301 ccgtcgggcg ccagcacgtg gatgagcgag aagaccttga aggagttgat gacgccgatg
      361 aggagcacga agtaggtgac gggcgagagg agaggcaggg tgatgtacct gaagcgttgc
      421 cagatcgtgg cgccgtcgat ctcggccgct tcgtagtaga cgcggccgat gccctgcagg
      481 ccggccagga agatcacgat gttgtagccc aggcctttcc agagcgccac gaggacgacc
      541 gacggcatgg ccaggctcgg gtcgcgcagc cagcgctggc tcgggagccc gaggctgttc
      601 agcaccgtgt tcaagaagcc gctgctcggg ttgtagagca cgcgccagat ggcggcggcg
      661 gcgaccatcg aggtcaccac gggcatgaag agcgccacgc ggaagaagct catcccccgg
      721 agcttctgat tgaggagcag ggcgacgagg agcgagagca ccatgtcgat cggaagcttc
      781 aacaccatga agtagaggga gttcttgaga gcgttatgga agtcgctcga cgtgagcgtg
      841 tccacgtagt tcttggcgcc cacgaagctg gtcgtcgcgc tgcctgcctg ccagttcgtg
      901 aagctgatgc cgaaggccca caggatcggg aagtagacga acgctatgag caccaggagc
      961 gagggcgcca ggaacgacag ggccatgagc atctcgcgcc gcgagttcgg ccggtggaag
     1021 aagggggcga tctccgaagc ccaggctccg aggc
//
LOCUS       DAOQYE010000962         1051 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_193868_length_1051_cov_3.758032, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000962 DAOQYE010000000
VERSION     DAOQYE010000962.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_193868_length_1051_cov_3.758032"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1051
                     /gene="glpK"
                     /locus_tag="PKN52_12610"
     CDS             <1..>1051
                     /gene="glpK"
                     /locus_tag="PKN52_12610"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: protein motif:HMM:NF000756.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HNR00838.1"
                     /translation="AIVWQDRRTAPEVERLRADGAEAVVRAKTGLLLDPYFSATKIAW
                     LLDNVPGARERAQAGELAFGTVDTWLTAKLTGGREHVTDVSNASRTQLFDIHGCDWDD
                     ELLELFRVPRALMPRVVPTSGRIAEVAEGPLAGAPIAALVGDQQAATFGQACFAPGQA
                     KATYGTGAFLVLNTGAEARPSANQLLTTVAWSEGGRLEYALEGSIFMAGAVVQWLRDG
                     LGIIREASEVGKLAAGVPDSGGVVFVPAMTGLGAPHWDSDARGTIVGLTRGTTAAHLA
                     RAALEGVALQVADVVDAMQADAAGEVRELRVDGGASRSDELMQLQADVLGVDVVRSAQ
                     SETTALGAAYLAGLAV"
BASE COUNT          146 a          372 c          386 g          147 t
ORIGIN      
        1 cgccatcgtg tggcaggaca ggcgaaccgc gccagaggtc gagaggctcc gcgccgacgg
       61 agccgaagct gtcgtccgcg ccaagaccgg actgctcctc gacccgtact tctccgccac
      121 caagatcgct tggctgctcg acaacgtgcc gggcgcccgc gagcgcgccc aggcgggcga
      181 actcgccttc ggcaccgtcg acacgtggct caccgcgaag ctgaccggcg gccgcgaaca
      241 cgtgaccgac gtgagcaacg cttcgcgaac gcagctcttc gacatccacg gctgcgactg
      301 ggacgacgag ctcttggaac tcttccgcgt gccccgcgcg ctcatgccga gggtcgtgcc
      361 cactagcggg cgcatagccg aggtcgccga aggtccgttg gcgggagcgc ccatcgcggc
      421 cctcgtcggc gaccagcagg ctgccacgtt cggccaggcg tgcttcgcgc ccggccaagc
      481 caaggcgacc tacggcacgg gcgcgttcct ggtcctgaac accggcgcgg aagcgcgccc
      541 ctccgccaac cagctgctca cgacggtcgc ctggtcggaa ggcggtcggc tcgagtacgc
      601 gctggaaggc agcatcttca tggctggcgc ggtggtgcag tggttgcgcg acgggcttgg
      661 catcatccgc gaggcgagcg aggtcggcaa gttggccgcc ggcgtgcccg actccggggg
      721 tgtcgtgttc gttcccgcca tgaccggcct cggcgcgccg cattgggact cggacgcgcg
      781 ggggaccatc gtcggcctca cgcggggcac cacggctgcg cacctggccc gggccgccct
      841 cgagggcgtc gccctgcagg tcgcggatgt cgtcgacgcc atgcaggcgg atgcggccgg
      901 tgaggtgcgc gagttgcggg tcgacggcgg ggcctcgcgc tccgacgagc tgatgcagct
      961 ccaggccgac gtgctaggag tggacgtcgt gcgctcggcg cagagcgaga cgaccgcgct
     1021 cggcgccgcc tacctggccg ggttggcggt g
//
LOCUS       DAOQYE010000963         1050 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_194226_length_1050_cov_3.386935, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000963 DAOQYE010000000
VERSION     DAOQYE010000963.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1050
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_194226_length_1050_cov_3.386935"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            149..>1050
                     /locus_tag="PKN52_12615"
     CDS             149..>1050
                     /locus_tag="PKN52_12615"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNR00839.1"
                     /translation="MEMIRSPLDRRAEGNKARLLQQTQSRVLEMIARGADLGDVLNEV
                     MLFLEQQLEGVRTSVLLMDRGGGHLSHGAAPSLPDSYNAHLNGLPIGPRVGSCGTAAY
                     YGEEVVVTDIATDERWVGSSELPLAHGLRACWSTPLLSPEGEVVGTFAVYYLEPRGPT
                     EEERAFVRVAAHLAEVAVTNRRRLEDLHERERSIGRMAGFRSGIITMMEQGLVTPYGP
                     DLYQHMLDQAVSSIPGAQAGALLLRSDDGHYAFAAANGHDFADLRRIRFSPAGAGFGV
                     PVESPHPQVVVDPVMDPELTPEERA"
BASE COUNT          167 a          374 c          362 g          147 t
ORIGIN      
        1 gcaaggtcgc gcagccgtgg tcggggagga ccggctaggc tcggcctcgg tcggcccccg
       61 cgccggtatg gcgcaagtct cacgggtgac gacccgaaca tctgcaacaa tgacgctgac
      121 cggccctggg ggatgccgga gtacccgtat ggagatgatc cgctccccac tagatcgtcg
      181 cgcggagggg aacaaggcgc gtctgctgca gcagacgcag agccgcgtct tggagatgat
      241 cgcccgcggc gccgacctgg gcgacgtcct gaacgaggtc atgctgttcc tggagcagca
      301 actcgaaggg gtccgcacgt cggtcctgct catggaccgc ggaggcgggc acctatccca
      361 cggcgccgcg ccaagcctgc ccgacagcta caacgcccac ctgaacggac tcccgatagg
      421 cccgcgcgta ggctcgtgcg gcaccgccgc ctactacggt gaagaggtgg tcgtcaccga
      481 catcgccacc gacgagcggt gggtcgggag cagcgaactg cccttggcgc acggcctgcg
      541 ggcctgttgg tcgacgccgc tactcagccc cgagggcgag gtggtcggga cgttcgccgt
      601 ctactacctg gagccgcgcg ggcccaccga ggaggagcgc gcgttcgtgc gggtggccgc
      661 ccacctggcc gaggtcgccg tcaccaaccg ccgccggctc gaggacctcc acgagcgcga
      721 gcggagcatc gggcgcatgg cggggtttcg cagcggcatc atcaccatga tggagcaagg
      781 cctcgtgacc ccgtacggtc ccgacctcta ccaacacatg ctcgatcagg ccgttagctc
      841 catccctggc gcgcaggccg gtgcgctcct gctgcggagc gatgacgggc actacgcctt
      901 cgccgcggcg aacggacacg acttcgccga cctgcgccgc atccgcttct ccccggccgg
      961 cgcgggcttc ggcgtgcccg tggagagccc gcatccgcag gtcgtcgtcg acccagttat
     1021 ggacccggaa ctgacccccg aggagcgcgc
//
LOCUS       DAOQYE010000964         1050 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_194233_length_1050_cov_3.246231, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000964 DAOQYE010000000
VERSION     DAOQYE010000964.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1050
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_194233_length_1050_cov_3.246231"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="PKN52_12620"
     CDS             <1..344
                     /locus_tag="PKN52_12620"
                     /inference="COORDINATES: protein motif:HMM:NF040249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00840.1"
                     /translation="TIEDKEWTRRYHSTDPSEKAFGGRVVVTLTDGTVIEDEIAVADA
                     HPLGARPFRRQQYVEKFRTLAEGVVAEAEQARFLELTARLGELTPAEVAQLNVVVEPE
                     KLGGEAPAGIF"
     gene            370..>1050
                     /locus_tag="PKN52_12625"
     CDS             370..>1050
                     /locus_tag="PKN52_12625"
                     /inference="COORDINATES: protein motif:HMM:NF012506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HNR00841.1"
                     /translation="MRPEPDAAAQPRAKKSVALSGVVAGNTALSTVGASGDELRYRGY
                     DVKELAGACEYEEVAHLLIHGTLPDATQLRAYKEKLRSLRGLPAAVKTVLEAIPAAAH
                     PMDVLRSGVSALGSVLPENEAHPGAGARDIADRLVASLGSMLMYWYHWSHKGELIDVE
                     TDDDTVGGHFLRLLHGKEPKASWVRAMHRSLILYAEHEFNASTFTCRMIAGTGSDVYS
                     AVCGGIGAL"
BASE COUNT          170 a          379 c          361 g          140 t
ORIGIN      
        1 gcacgatcga ggacaaggag tggacgaggc gctaccactc caccgacccg agcgagaagg
       61 cgttcggcgg ccgcgtcgtc gtcaccctga ccgacggcac ggtcatcgag gacgagatcg
      121 ccgtcgccga cgcccacccg ctcggcgcca ggccgttccg ccgccagcag tacgtcgaga
      181 agttcaggac gctcgccgag ggcgtcgtgg cggaggccga gcaggccagg ttcctggagc
      241 tgacggcgcg cctaggcgag ctgacgccgg ccgaggtcgc tcagctgaac gtggtcgtcg
      301 agccggagaa gctcggcggc gaggcaccgg ccgggatctt ctagaagagc tttcgtggag
      361 gtgagttaga tgcgtccaga accagacgcc gcggcccagc cgcgcgccaa gaagtccgtg
      421 gccctatccg gcgtggtggc cggcaacacg gcgctgtcaa ccgtcggcgc cagcggcgac
      481 gagctgcgct accggggcta cgacgtcaag gagctggcag gcgcgtgcga gtacgaggag
      541 gtcgcgcacc tgctcatcca tggcactctg ccggacgcca cccagctccg ggcctacaag
      601 gagaagctgc gctccctgcg cggcctcccc gccgccgtca agaccgtgct ggaggccatc
      661 cccgccgcgg cccaccccat ggacgtgctg cgcagcggcg tctcggccct cgggtcggtc
      721 ctgcccgaga acgaggcgca cccgggagcg ggcgcgcgcg acatcgccga ccgcctcgtc
      781 gcctcgctcg gctccatgct catgtactgg taccactggt cgcacaaggg cgagctgatc
      841 gatgtcgaga cggacgacga caccgtcggc ggccacttcc tcaggctcct gcacggcaag
      901 gagccgaagg cgagctgggt gcgcgccatg caccgctcgc tcatcctcta cgccgagcac
      961 gagttcaacg cctccacctt cacctgccgg atgatcgccg gcaccggctc cgacgtctac
     1021 tcggccgtgt gcggcggcat cggcgccctg
//
LOCUS       DAOQYE010000965         1048 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_194993_length_1048_cov_2.314199, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000965 DAOQYE010000000
VERSION     DAOQYE010000965.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1048
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_194993_length_1048_cov_2.314199"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1048
                     /locus_tag="PKN52_12630"
     CDS             <1..>1048
                     /locus_tag="PKN52_12630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNR00842.1"
                     /translation="VNGVTGILSLGHHGFMLIGGYVTGLLVLPSATRDNLARSARSEL
                     SDFAMGLSVENWFRALGLDSLTTPEMLWARFLVAVLVGGLVAALFGIVVGFPSLRLRG
                     DYLAIVTFAFGEAVRLLASTPMLSSFTNGALGFAGVPSDIGKSVWWTFGFLAVTVFVM
                     ARLKFSSYGRVLQGIREDEIASEAMGVNTAYHKVLAFAISAFFAGVAGGLWVSWVGTA
                     RLDLFLFFLTFYFLVAISVGGTGSITGVLLGTALVVWVRQYGDPLEQTYPLSTWAVAA
                     GLVLLAVAILAFVLRRVRRLSPRVAPVVYVPAVLGVAAVLVGLLGKGMPALQATWQGF
                     GMRAITLSLLLMAIM"
BASE COUNT          107 a          379 c          348 g          214 t
ORIGIN      
        1 cgtcaacggc gtcacgggca tcctgtccct cggccaccac ggcttcatgc tcatcggcgg
       61 ctacgtgacc ggcctgctgg tccttccgtc ggccacgcgc gacaacctcg cgcgcagcgc
      121 gcgcagcgaa ctctccgact tcgccatggg cctctccgtc gagaactggt tccgcgccct
      181 cgggctcgac tcgctcacga ccccagagat gctctgggcg cgtttcctgg tggccgtcct
      241 ggtcggtggc ctcgtggccg cgctcttcgg gatcgtcgtc ggcttcccct cgctgcggtt
      301 gcggggcgac tacctggcca tcgtcacgtt cgcgttcggc gaggcggtca ggctcctcgc
      361 ctcgacgccc atgctgtcgt cgttcaccaa cggggctctc ggcttcgcgg gcgtgccgtc
      421 cgacatcggc aagagcgtct ggtggacgtt cggcttcctc gccgtcacgg tcttcgtcat
      481 ggcgcgcctc aagttctcgt cgtacgggcg cgtgctgcag ggcatcaggg aggacgagat
      541 cgccagcgag gcgatgggcg tcaacaccgc gtaccacaag gtgctcgcct tcgccatctc
      601 cgcgttcttc gcgggcgtgg cgggcggcct gtgggtcagt tgggtcggca ccgcgcgcct
      661 cgacctcttc ctgttcttcc tcaccttcta cttcctcgtc gccatcagcg tcggcggcac
      721 gggctcgatc accggcgtgc tcctaggcac ggcgctcgtc gtgtgggtgc ggcagtacgg
      781 cgacccgctc gagcagacgt acccgctctc gacctgggcc gtggcggccg gtctcgtgct
      841 cctcgcggtc gccatactgg cgttcgtgct gcggcgcgtc aggcggctct cgccgcgcgt
      901 ggcgcccgtc gtctacgtgc cggccgttct cggtgtcgcg gcggtgctcg tcggcctcct
      961 cggcaaaggt atgccggcct tgcaggcgac gtggcagggc ttcggcatgc gggcgatcac
     1021 gctttcgctc ctgctcatgg ccatcatg
//
LOCUS       DAOQYE010000966         1045 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_195879_length_1045_cov_3.702020, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000966 DAOQYE010000000
VERSION     DAOQYE010000966.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_195879_length_1045_cov_3.702020"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1036)
                     /locus_tag="PKN52_12635"
     CDS             complement(<1..1036)
                     /locus_tag="PKN52_12635"
                     /inference="COORDINATES: protein motif:HMM:NF019040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HNR00843.1"
                     /translation="MVGTGALLLSRPLFPNLPFVAFAQTPREVDTLVVIFLRGGMDSL
                     AAIVPVGEGAAFYDRRSQTAVPESRVIDLDGFFGFHPALGRLEDIYRGGHLAVVHAAG
                     SPDPSRSHFDAMEYMERGTPGVRVTETGWLTRHLQSVPWTNDSPFRAVAMGALLPSSL
                     QGPEASLALESLDGFELNVPDHLRPDLENGLRELYRNVGAGPAFQREAESVFSTMRDL
                     RRMADDGGLERGNSGALGYPETSFGQALRQVAQLIRADVGLEVACVDLGGWDTHEEQG
                     GVEGQFADLLSELGAGLAAFYEDVAERSNRLTVVTMSEFGRKLEETASGGTDHGRGGS
                     MLVMGGGVSGG"
BASE COUNT          165 a          380 c          362 g          138 t
ORIGIN      
        1 ccccaccgct cacgcccccg cccatgacga gcatggagcc accgcgcccg tggtcggtcc
       61 cgccgctcgc ggtctcctcg agcttgcgac cgaactcgct catcgtcacg accgtcagac
      121 ggttggagcg ctccgccacg tcctcgtaga aggccgcgag cccggcgccg agttcggaga
      181 gcaggtcggc gaactgtccc tcaacgccgc cctgctcctc gtgggtgtcc cagccgccga
      241 ggtcgacgca cgcgacctcg agtccgacgt cggcgcggat cagctgggcc acctgccgga
      301 gcgcctggcc gaagctcgtc tccgggtacc cgagcgcccc ggagttgcca cgttccaagc
      361 cgccgtcgtc ggccatgcgc ctaaggtcgc gcatcgtgct gaagacgctc tcggcctcgc
      421 gctggaacgc cggacctgcg ccgacgttcc ggtagagctc gcgcagcccg ttctccaggt
      481 cgggccgcag gtgatccggg acgttcagct cgaagccgtc cagtgactcg agcgcgaggg
      541 acgcctccgg cccctgcagc gaggagggga gcagcgcgcc catcgccacc gcccggaagg
      601 gcgagtcgtt ggtccacggg acggactgca ggtggcgcgt gagccatccg gtctccgtga
      661 cacgcacgcc aggcgtgccg cgctccatgt actccatggc gtcgaaatgc gagcgcgacg
      721 gatcgggtga accggcggcg tgcaccacgg cgaggtggcc gccgcgatag atgtcctcca
      781 ggcgcccaag ggcagggtgg aagccgaaga agccgtccag gtcgatgacc cgactctcgg
      841 gcacggccgt ctgcgagcgc cggtcgtaga aagcggcgcc ctcgccgacg gggacgatgg
      901 cggccaggct gtccataccg ccccgcagga agatgacgac gagcgtgtcg acctcacgcg
      961 gcgtctgagc gaacgccacg aacggcaggt tcgggaagag cggtcgcgac aggagcagcg
     1021 cgccggtccc gaccatgccc ttcag
//
LOCUS       DAOQYE010000967         1042 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_196925_length_1042_cov_3.031408, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000967 DAOQYE010000000
VERSION     DAOQYE010000967.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_196925_length_1042_cov_3.031408"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="PKN52_12640"
     CDS             <1..270
                     /locus_tag="PKN52_12640"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HNR00844.1"
                     /translation="ARPGGSLFGVTVGQTKAQIEVDVRATDIITGEVIVSMKGSGEAS
                     SPNVNINNVPLAGNVRYDDPTVGPLFAQAANSAVSQVVAALRAKF"
     gene            complement(370..>1042)
                     /locus_tag="PKN52_12645"
     CDS             complement(370..>1042)
                     /locus_tag="PKN52_12645"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HNR00845.1"
                     /translation="HPLGTVAVREGPAMAATDSFRFVVKGRGGHAAYPHVCIDPIVAA
                     SQLVLALQTLVSRETDPVDQSVISITSFHGGTAFNIIPEDVELKGTLRTFDAATRSRL
                     HERIVAVAEASCALARCSLEHEWITGTPAVVNDAESVERFRKVAARVVGPDNVTVQAP
                     IMGGDDMALWLAQARGCYFFVGTSSDADTSWAHHHPRFDIDERGLEVAVTVLASGVAD
                     MMSGD"
BASE COUNT          167 a          361 c          356 g          158 t
ORIGIN      
        1 gcccgcccgg gcggcagtct cttcggcgtg accgtcggcc agaccaaggc ccagatcgag
       61 gtggacgtgc gcgcaacgga catcatcaca ggcgaggtca tcgtctccat gaaggggtcc
      121 ggcgaggcga gcagccccaa cgtcaacatc aacaacgtcc cgttggccgg caacgtcagg
      181 tacgacgacc cgaccgtcgg cccgctcttc gcgcaggccg ccaactcggc agtcagccag
      241 gtagtggccg ccctgcgcgc taagttctaa gcgcctcgcg gttccaagcg acgggccgat
      301 cgctcaagcc tcacggcggc gaccggcccg ttcttcgttc ttcgttcgtc gtgagccgct
      361 accgaccgat cagtctccgc tcatcatgtc ggccacgccg gaagcgagca cggtgacggc
      421 cacctcgagg ccgcgctcgt cgatgtcgaa gcgcgggtgg tggtgcgccc acgaggtgtc
      481 ggcatcggag ctcgtgccga cgaagaagta gcaaccgcgc gcctgggcga gccagagcgc
      541 catgtcgtct ccgcccatga tgggcgcttg gacggtcacg ttatcggggc cgacgacgcg
      601 cgccgccacc ttgcgaaagc gttcgaccga ctcggcgtcg ttgaccaccg cgggcgtgcc
      661 ggtgatccat tcgtgctcga gcgagcacct ggcgagcgcg cacgaggcct ccgccaccgc
      721 gactatgcgc tcgtgcaggc gcgaccgcgt agcggcgtcg aaggtgcgga gcgtgccctt
      781 gagctcgacg tcctccggga tgatgttgaa ggcggtgccg ccatggaagc tcgtgatgct
      841 gatgaccgac tggtccaccg ggtccgtctc acggctcacg agcgtctgca acgccagtac
      901 cagctgtgac gccgccacga tcgggtcgat gcacacgtgc ggataggcgg cgtggccgcc
      961 gcgccccttg actacgaacc ggaacgagtc ggtcgcggcc atggccggcc cctcgcgcac
     1021 ggcgacggtg ccgagcgggt gg
//
LOCUS       DAOQYE010000968         1042 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_197008_length_1042_cov_2.311044, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000968 DAOQYE010000000
VERSION     DAOQYE010000968.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_197008_length_1042_cov_2.311044"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            37..>1042
                     /locus_tag="PKN52_12650"
     CDS             37..>1042
                     /locus_tag="PKN52_12650"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HNR00846.1"
                     /translation="MAAAVFTVYALIDRQPALGFMAVVVVGVPLGWWLRSAGSADAEP
                     SRREALAAVMLSWVVLPALGALPYALGHHMSLLDAAFESVSGFTTTGATTITDFSAVA
                     PSLMLWRALTQWVGGVGILVLFLAVFPQLAIAGRQLFNAKVPSPQEERLTPKLRQTAM
                     AVVGLYTALTLVCSIAYYLTGMGTFDAVAHALGTLAAGGFSPNAESFIGYGAAAQWIA
                     VIFMFLSGTSFFLVYGTLTGRKRNMLRDTEFRVYLAIQLVSGALLAVLLLGEDLAPLD
                     AIRHGLFQATSMVVTAGFASLDYLRTPAAAVETAWKAHSLLDYLPSLAVGIGGRLLDL
                     G"
BASE COUNT          138 a          382 c          325 g          197 t
ORIGIN      
        1 ccgtatgtgc ttggaacggg tgttcaggcg ctcagcgtgg ccgccgccgt cttcaccgta
       61 tacgcgttga tagaccgcca gccggcgctc ggcttcatgg ccgtcgtcgt ggtcggcgtg
      121 ccgctaggct ggtggctgcg cagcgccggc agcgccgatg ccgaacccag ccgccgcgaa
      181 gccctggctg ccgtgatgct ctcatgggtg gtgctgccgg cgctcggcgc actaccgtac
      241 gcgctcggcc accacatgtc gctgctcgat gccgcgttcg agtccgtcag cggcttcacc
      301 accaccggcg ccaccaccat cacggatttc tccgccgtgg cgccctcact gatgctgtgg
      361 cgcgctttga cgcaatgggt cggtggcgtc ggcatcctcg tgctcttcct cgccgtcttc
      421 ccgcagctcg ccatcgcggg tcgacaactc ttcaacgcca aagtgcctag cccgcaagag
      481 gagcgcctga cacccaaact gcggcagacg gcgatggcgg tcgtagggct gtacacggcc
      541 ctcacgctcg tgtgctccat cgcctactac ctgactggca tggggacgtt cgacgccgtc
      601 gcccacgcgc tcgggacgtt ggcggccgga gggttcagcc ccaacgccga gagtttcatc
      661 ggttacggcg cggctgccca gtggatcgcc gtcatcttca tgttcctatc cggcacgagc
      721 ttcttcctcg tctacggcac cctgacaggt cgcaagcgca acatgctgcg cgacaccgag
      781 ttccgcgtct acctcgccat ccagctcgtc tccggggcat tgctcgccgt gctgctgctc
      841 ggggaggacc tcgcgcctct cgacgcgatc cggcacgggc tctttcaggc caccagcatg
      901 gtcgtcacgg ccggtttcgc ctccctcgac tacctgcgta cgcccgccgc ggccgtggag
      961 acggcatgga aggcgcactc gctcctcgac tacctgccct ccctggccgt ggggatcggg
     1021 ggacgcctcc tggacctggg gc
//
LOCUS       DAOQYE010000969         1041 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_197260_length_1041_cov_3.085193, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000969 DAOQYE010000000
VERSION     DAOQYE010000969.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_197260_length_1041_cov_3.085193"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(193..>1041)
                     /locus_tag="PKN52_12655"
     CDS             complement(193..>1041)
                     /locus_tag="PKN52_12655"
                     /inference="COORDINATES: protein motif:HMM:NF015649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HNR00847.1"
                     /translation="EAAPADTTDLDTLVSLTNAGLRVLPHLLPLAALPRHRPDLAVAY
                     PFTDVLAAGWEAAIEARLDEVPPLRIDLLGRFDVTVLGRRVTLTPRHREIVTLLALGY
                     DRTSVGLALWPEADKRKVQNNLQVQLTMLRRELEPWGVRTYLSDAGLERTVCDVAELR
                     AALKAGDADEAARLYREPLAAGIELDALDEERYQLREDVLAVLKGGAGSAVAAGDFPA
                     ALNLLDHVMRIDPLDEDALAEQVRVLVRVGKTREAHRRYRLFAERLRDQTGLEPQPAT
                     GAALGQ"
BASE COUNT          146 a          381 c          363 g          151 t
ORIGIN      
        1 gcgtcgggga caccgcccat gaacccgtcc acgaacgtgc cggcggcacg gcagcagccg
       61 acgctacagg caccgcatgg caagggctgg ccttccggat gagcttcgct acctacggcc
      121 tgtcggaccc gctacctgac tgagcggaac accgtgcggt ggtcgacggc tccaggctgg
      181 gcggcccctt cctcactgcc ctagcgccgc gcccgtcgcg ggctgcggct ccaagcccgt
      241 ctgatcgcgc aggcgctcgg cgaacagccg gtaccttcta tgggcctcgc gcgtcttgcc
      301 gacccgcacc agcacccgca cctgttcggc cagagcgtcc tcgtccagcg ggtcgatgcg
      361 catgacgtgg tcgagcaggt tgagcgcggc ggggaagtct ccggccgcga ccgcggaccc
      421 ggcccctccc ttgagcacgg ccaacacgtc ctcacggagt tggtaccgct cctcgtccag
      481 cgcgtccaac tcgatgccgg cggccagcgg ctcgcggtag aggcgggcgg cctcgtcggc
      541 gtcaccggcc ttcagggccg cgcgcagctc cgccacgtcg cacacggtcc gctcgagccc
      601 ggcatcgctg aggtaggtcc tgacgcccca cggttcgagc tcgcgccgga gcatcgtcaa
      661 ctgcacttgg aggttgttct gcaccttgcg cttgtcggcc tcgggccata gggccaggcc
      721 aacgctggtc ctgtcgtaac ccaacgccag cagcgtcact atctccctat gccgcggggt
      781 gagcgtgacg cgccgcccca ggacggtcac gtcgaagcgg ccgaggaggt cgatgcgcag
      841 cggcggcacc tcgtcgagcc gggcttcgat cgccgcttcc cagccggccg cgaggacgtc
      901 ggtgaagggg taggccaccg ccaggtcggg ccggtggcgc ggcagcgccg ccaacggcag
      961 gaggtgcggg agcacgcgca accccgcgtt ggttagagag accagcgtgt ccaggtcggt
     1021 ggtgtcggcg ggcgcggcct c
//
LOCUS       DAOQYE010000970         1041 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_197351_length_1041_cov_2.200811, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000970 DAOQYE010000000
VERSION     DAOQYE010000970.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_197351_length_1041_cov_2.200811"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="PKN52_12660"
     CDS             <1..448
                     /locus_tag="PKN52_12660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNR00848.1"
                     /translation="VARVLSGGRIGIGAMGIGLGRAALEKAAKYALERRQFGQTLSRH
                     QAVAFKLAEMATELEAARLLVAKAAALRDAGRDHTLAAAMAKLKGSVAGVNACDSAIQ
                     VLGGYGYLRDYEVERYWRDARLTRIGEGTDEIQHLIIAREYLRQLG"
     gene            complement(469..>1041)
                     /locus_tag="PKN52_12665"
     CDS             complement(469..>1041)
                     /locus_tag="PKN52_12665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HNR00849.1"
                     /translation="PELLIELNGGVPDLAAARDHLRRVDGVMLGRSVYEDPFVLAAAD
                     EFLADDAHAAGVAHAAGAAPMTGAAAEPKVIGTPLTRRAVVEATYPYVEEQLLAGAPL
                     GALTRHMLSLFRSQPGGRTWRRIISERSHRAGAGVEVLQAALAALPEGVADAPVTHGA
                     AEDPAQGTGEPCRGAATATATATATATLTV"
BASE COUNT          147 a          371 c          389 g          134 t
ORIGIN      
        1 cgtggcgcgc gtgctgagcg gcgggcgtat cggcatcggc gccatgggca tcggcctcgg
       61 acgggccgcc ctcgagaagg ccgccaagta cgcgctcgaa cgacggcagt tcgggcagac
      121 gctctcccgc caccaagccg tagccttcaa gctggccgag atggcgaccg agttggaggc
      181 cgcgaggctg ctcgtcgcga aggccgccgc gctgcgcgac gccggacgcg atcacacctt
      241 ggccgccgcc atggcgaagc tgaaggggag cgtcgccggc gtgaacgctt gcgactcggc
      301 catccaggtg ctgggcggct acggctacct gcgcgactac gaggtggagc gctactggcg
      361 cgacgcccgc ctcacccgta tcggcgaggg caccgacgag atccagcacc tgatcatcgc
      421 gcgcgagtac ttgaggcaac tcggttagct gagcgcaacc ggagcagatc agacggtcag
      481 ggtcgcggtt gcggtcgcgg ttgcggtcgc ggtagccgcc ccgcggcagg gctcgcccgt
      541 cccctgcgcc gggtcttcgg cggccccgtg cgtgacgggc gcgtccgcca cgccttcagg
      601 cagcgcggca agcgcggcct gcagcacctc gaccccggcg ccagcacggt gggaccgctc
      661 ggagatgatg cgccgccagg tccttccgcc cggctgggag cggaacagtg acagcatgtg
      721 gcgcgtcagc gcccccaacg gcgcgccggc gagcagttgc tcctcgacgt acgggtaggt
      781 ggcctccacg acggcgcgcc gggtaagcgg cgttccgatg accttcggct ccgcggcggc
      841 gccggtcatg ggggcggcgc cggccgcgtg ggcgacgccc gccgcgtggg cgtcgtccgc
      901 caggaactcg tccgccgcgg ccagaacgaa cgggtcctcg tagacggagc gacccagcat
      961 cacgccgtct acgcgccgca ggtggtcgcg tgcggcggcc agatcgggca cgccgccgtt
     1021 gagctcgatc agcagctccg g
//
LOCUS       DAOQYE010000971         1040 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_197617_length_1040_cov_2.726904, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000971 DAOQYE010000000
VERSION     DAOQYE010000971.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_197617_length_1040_cov_2.726904"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1040)
                     /locus_tag="PKN52_12670"
     CDS             complement(<1..>1040)
                     /locus_tag="PKN52_12670"
                     /inference="COORDINATES: protein motif:HMM:NF040252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HNR00850.1"
                     /translation="PLLSNAFEIPFPDIRPEHVGPGLRTALARAEAGLSELRAGAAEP
                     TYANTVQALDDVVEGVVRPYVLARHLIGVATTPELRQAFNEVLPEVSGFLARLGTDPA
                     LWQTVSRYAATPEAAGLDDVRRRNLDKVLTEFRQSGASLPEAERKRVETLRVELSQLT
                     TKFAENILDATNAFELLIEDEDAIAGLPDGPKRRARADASARGLTGFRFTLQAPSYMS
                     FVKYVDDRELRRRMHEAYNSVGTSEPHDNRGIMRQILAKRRELAVVLGYADYADLQTE
                     DRMIKSGLRAAQFEEELVAKTRPYFEADMAELERFAVRELGVPRLEPWDVLYASEKLR
                     LARFSEAYSTSH"
BASE COUNT          151 a          338 c          381 g          170 t
ORIGIN      
        1 cgtgggacgt gctatacgcc tccgagaacc gcgccaggcg cagcttctcg gaggcgtata
       61 gcacgtccca cggctccaga cgcgggacgc cgagctcgcg caccgcgaag cgctcgagct
      121 ccgccatgtc ggcctcgaag tatggcctcg tcttggcgac gagctcctcc tcgaactgag
      181 ccgcgcgcag gccgctcttg atcatgcggt cctccgtctg gaggtccgcg tagtcggcgt
      241 agccgagcac gaccgcgagc tcgcggcgct tggcgaggat ctggcgcatg atcccgcggt
      301 tgtcgtgagg ctccgacgtg ccgacggagt tgtacgcctc gtgcatgcgc ctacgcagct
      361 cacggtcgtc gacgtacttg acgaacgaca tgtaggaggg cgcttggagg gtgaagcgaa
      421 agccggtgag gcctcgggcg gacgcgtcgg cgcgtgccct gcgcttgggg ccgtccggca
      481 ggccggcgat cgcgtcctcg tcctcgatga gcagttcgaa ggcgttggtg gcgtccagga
      541 tgttctcggc gaacttggtc gtgagctggg acagctcgac acgcagggtt tcgacgcgct
      601 tgcgctcggc ctcgggcaag ctcgcgccgg actgccggaa ctccgtcaga accttgtcca
      661 ggttccggcg cctgacgtcg tccagacccg cggcttccgg cgtggccgcg taacgcgaca
      721 cggtttgcca gagcgccggg tccgtgccga ggcgcgcgag gaagccgctg acctcgggga
      781 gcacctcgtt gaaggcctgg cgcagttcgg gcgtggtagc cactccgatc agatggcggg
      841 ccagtacgta cggcctcacg acgccctcga cgacgtcgtc gagggcctgg accgtgttcg
      901 cgtaggtcgg ttcggcggca ccggctcgca actcggagag cccggcctcg gcccgagcga
      961 gcgcggtgcg caggcccggt ccgacgtgct cgggccggat gtcggggaaa gggatctcga
     1021 aagcgttcga gagcagcggg
//
LOCUS       DAOQYE010000972         1038 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_198312_length_1038_cov_2.334690, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000972 DAOQYE010000000
VERSION     DAOQYE010000972.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1038
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_198312_length_1038_cov_2.334690"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="PKN52_12675"
     CDS             complement(<1..219)
                     /locus_tag="PKN52_12675"
                     /inference="COORDINATES: protein motif:HMM:NF018460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HNR00851.1"
                     /translation="MTDASGTLFAVFAAVIGSLIGSFGNVVVWRLPRGESVAFPPSHC
                     PNCNHRLGPLELVPVLSWLALRGRCRHCH"
     gene            324..836
                     /locus_tag="PKN52_12680"
     CDS             324..836
                     /locus_tag="PKN52_12680"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HNR00852.1"
                     /translation="MRRLTLIGLAVAAMAALLVANTFASTLSAQARQTKVVFIDSQAV
                     IRAHPSGAQVDEIRNRAEAEVKELTDSISALEAKVAAGQTLSPDESERYATLRSTLSA
                     VQTRYLNEINEAAAPAVAAANAAIAELAKENGYTIVIDMVEAANQRLVVYSDDDLNIT
                     QLAIDRVKAN"
     gene            complement(859..>1038)
                     /locus_tag="PKN52_12685"
     CDS             complement(859..>1038)
                     /locus_tag="PKN52_12685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00853.1"
                     /translation="LDVPEVLLSGHHGRVAEWRRREALLRTFRRRPDLLERAPLTEEE
                     RALVAGWKAEAEREK"
BASE COUNT          187 a          363 c          338 g          150 t
ORIGIN      
        1 gtggcagtgg cggcagcgac ctctgagggc aagccacgag agcacaggga cgagttccaa
       61 ggggccgagc cggtggttgc agttggggca gtgactcggc gggaaggcta cggactcgcc
      121 gcgtggcagg cgccacacga ccacgttgcc gaacgagccg atgaggctcc ctatgacggc
      181 ggcgaagacc gcgaagaggg ttccgctggc gtcggtcaag accgcctcgc tacctgcgtt
      241 ggtgggagtg tgaaggtggc gttcagcacg ggagcttgcc tcgcgggcgt tctcatcatc
      301 gcggtggtaa gctgccaccc gctatgagaa gactcaccct gatcgggctc gccgtggccg
      361 ccatggctgc actactcgtt gcgaacacgt tcgcctcaac cctcagcgct caggcgcgcc
      421 agacgaaggt cgtgttcatc gactcgcaag ccgtgatccg cgctcacccg tcgggtgccc
      481 aggtagacga gatccgtaac cgtgccgagg ccgaggtcaa ggagctcacg gacagcatca
      541 gcgctctcga agccaaggtc gctgccggcc agaccctgag ccccgacgag agcgagcgct
      601 acgccaccct gcgctcgacg ctctccgccg tgcagacgcg ctacttgaac gagatcaacg
      661 aggccgccgc cccggccgtc gcggccgcca acgccgccat cgccgaactg gccaaggaga
      721 acggctacac gatcgtgatc gacatggtcg aagctgccaa ccagcgcctc gtcgtctact
      781 ccgacgacga cctcaacatc acccagctcg ccatcgacag ggtcaaggcc aactgaccgt
      841 gtgcgagtga gccgaggctt acttctcccg ttccgcctcg gccttccaac cggccaccag
      901 cgcgcgctcc tcctccgtga ggggcgcgcg ctccagtaga tcggggcgcc tccggaacgt
      961 gcgcagcagg gcctcgcgcc tgcgccactc cgccacgcgc ccatggtggc cggagaggag
     1021 cacctcgggc acgtcgag
//
LOCUS       DAOQYE010000973         1038 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_198361_length_1038_cov_2.049847, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000973 DAOQYE010000000
VERSION     DAOQYE010000973.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1038
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_198361_length_1038_cov_2.049847"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1038)
                     /locus_tag="PKN52_12690"
     CDS             complement(<1..>1038)
                     /locus_tag="PKN52_12690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNR00854.1"
                     /translation="DVSPAAVIAQEEIFGPVIVSMTFRTPAEAVELANDTRYGLAASV
                     WTENVNLALDVAPKLKAGVVWVNCTNQFDAAAGFGGYRESGFGREGGREGMFEYLRPT
                     YEAALEPYAPAEVAPPAPAADVTAGLHDGASPAGIDRTPKLYVGGKQKRPDGNYVYPV
                     LSAAGVRVGDAPLGNRKDVRDAVEAALKGSAWGKATAHLRAQVLYYVAENLGVRAPEF
                     EERLTSLTGASRRAAAREVARSVERLFAAAAYADKYDGRVHPTPMRNVTLAMNEPYDV
                     LAVVCPDEAPLLAFVSLVAPALAMGNRVVAVPSARWPLLATDLYQVFDTSDVPGGAIN
                     IVTGVRDELAK"
BASE COUNT          131 a          375 c          379 g          153 t
ORIGIN      
        1 ccttggcgag ctcgtcgcgc acgcccgtca cgatgttgat ggcgcccccc ggcacgtcgg
       61 acgtgtcgaa cacctggtag aggtcggtgg cgaggagcgg ccagcgggcg ctcggcaccg
      121 ccaccacacg gttccccatg gcgagggccg gggcgacgag cgacacgaag gcgagtagcg
      181 gcgcctcgtc tggacagacg accgccagca cgtcgtaagg ctcgttcatg gcgagcgtga
      241 cgttgcgcat gggggtcgga tgcacgcggc cgtcgtactt gtcggcgtac gcggcggccg
      301 cgaagaggcg ctcgacgctc ctggcgacct cgcgcgccgc ggctcgccgc gaggcgcccg
      361 tgaggctcgt caggcgctcc tcgaactcgg gtgcccgcac gccgaggttc tccgccacgt
      421 agtagaggac ctgcgctcgc aggtgcgccg ttgccttgcc ccaggcgctc cccttcaggg
      481 cggcctcgac cgcgtcgcgc acgtccttgc ggttgccgag gggggcgtcc cccacccgca
      541 cgccggcggc gctgagcacc gggtagacgt agttgccgtc ggggcgcttc tgcttgccgc
      601 ccacgtagag cttgggggtg cggtcgatgc cggccgggga agcgccgtcg tgcaggcccg
      661 ccgtcacgtc ggcggcgggc gcgggtggcg ccacctcggc cggggcgtaa ggctcgagcg
      721 cggcctcgta cgtcgggcgc aggtactcga acatcccctc gcgcccaccc tcgcggccga
      781 agccgctctc gcggtaaccg ccgaagcccg cggccgcgtc gaactggttc gtgcagttga
      841 cccagaccac gccggccttg agcttgggcg ccacgtcgag ggcgaggttg acgttctcgg
      901 tccacacgct ggccgccagg ccgtagcggg tgtcgttggc cagttccacc gcctcggccg
      961 gcgtcctgaa cgtcatgctc acgatgaccg gcccgaagat ctcctcctgc gcgatgaccg
     1021 cggctggcga cacgtccg
//
LOCUS       DAOQYE010000974         1037 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_198574_length_1037_cov_2.885947, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000974 DAOQYE010000000
VERSION     DAOQYE010000974.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_198574_length_1037_cov_2.885947"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="PKN52_12695"
     CDS             complement(<1..440)
                     /locus_tag="PKN52_12695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00855.1"
                     /translation="MTNFVKLGRRALVVCVVLVAVSAAWAKSAYFVQADVVRGAIGAT
                     GAVCVFNGVFQQGEQIVWRAYVFDSETGEVLTPEQVEALGVQVFGVIDGDQKAELHFM
                     PHPPGSDDAEFFWAGGWQIPADYATGNYAWTLQVVDAAGNTATYT"
     gene            725..>1037
                     /locus_tag="PKN52_12700"
     CDS             725..>1037
                     /locus_tag="PKN52_12700"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNR00856.1"
                     /translation="MLEAYRTPDRLRARIAFHERYKGGGEDFHRRLFSLVTQRVEPAA
                     GARILEVGSGTGRLWSMNAERVPPGWRITLTDASEGMVNAAREAAADAGLAATVLRAD
                     VQ"
BASE COUNT          166 a          356 c          351 g          164 t
ORIGIN      
        1 gtgtaggtgg ccgtgttgcc ggcagcgtcg accacctgga gcgtccaagc gtagttgccc
       61 gtggcgtagt cggccgggat ctgccaaccg ccggcccaga agaactcggc gtcgtcactg
      121 cccggagggt gcggcatgaa gtggagctcc gccttctggt cgccgtcgat cacgccgaag
      181 acctgaacgc cgagcgcctc gacctgctcg ggtgtcagga cctcgcccgt ctccgagtcg
      241 aacacgtagg cgcgccacac gatctgctcg ccctgctgga agacgccgtt gaacacgcag
      301 accgcgcccg tcgcgccgat ggcgccccgg acgacgtccg cttgcacgaa gtacgccgac
      361 ttcgcccaag ctgcgctcac ggcgacgagg acgacgcaga cgactagcgc tctgcggccg
      421 agtttcacga agttggtcac tttgcacctc atccttcctg gtacccggcc cccaaggagg
      481 gcggccgcca aacgtgcccc ggacggtcgt cgctttcggg tcgagcttac gaactgtgag
      541 tctaatcctt tcgcgcatgc gcgtgcggtg acagggagcg taggtggcgt ccggttgcca
      601 tgctcacgaa gaaatggaca tgcgtgcgag cgaccctgca tagagcagcc gccttctaag
      661 cggtaggccg tccgatcagc gtgcggcgga agatcgcccc gcccctgcta tcctggcgac
      721 acccatgcta gaggcctata ggacgcccga ccgcctgcgt gcgcgcatcg cgtttcacga
      781 gcgttacaag ggcggcggcg aggacttcca ccggcggctc ttctccttgg tcacgcagcg
      841 cgtggagcca gcggccggtg cgcggatcct cgaggtcggc tcgggcaccg ggcgcctatg
      901 gagcatgaac gcggagcgcg tgccgcccgg ttggcgcatc actctgacgg acgcctccga
      961 gggcatggtg aacgccgcgc gcgaagcggc ggccgacgcc ggcctggcgg ccacggtgct
     1021 gcgcgccgac gtgcaac
//
LOCUS       DAOQYE010000975         1035 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_199264_length_1035_cov_2.641837, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000975 DAOQYE010000000
VERSION     DAOQYE010000975.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_199264_length_1035_cov_2.641837"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="PKN52_12705"
     CDS             complement(<1..392)
                     /locus_tag="PKN52_12705"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNR00857.1"
                     /translation="MRTLPLRNVPNHAAGHRGVDPRRAAEGDPMTLADPDRIPGLSPS
                     GYDRLGEFHDLFVDEARLRLRPAFESAFGRLEPSAVVLDLGAGTGLGVRHLARATRAR
                     IVAVEPSVTMRAVLLARVADDPALASRVT"
     gene            454..1005
                     /locus_tag="PKN52_12710"
     CDS             454..1005
                     /locus_tag="PKN52_12710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00858.1"
                     /translation="MLKAVVGLVRPSGRSWQALLPGPALAPAATNHRPRDFSEELVYD
                     FVLTLHNYLRWALLLVGLAALVLAWSGAASRGRWTPPQVNLGRLFTGLFDLQVLVGVL
                     LYAVLSPLTTGAFKNIGAAMKNPDLRFYVAEHLVGMLIAAVLVHIGAARAKRKDSPLQ
                     AAIFYTLAFVVVIVSIPWERPLF"
BASE COUNT          140 a          375 c          337 g          183 t
ORIGIN      
        1 gtgactctgc tagcgagcgc cggatcgtcc gcgacacggg cgagcagtac cgcgcgcatc
       61 gtcaccgacg gctctaccgc gacgatccga gcccgcgtcg cccgagcgag atgacgcacg
      121 cccaaccccg tgcctgcgcc gaggtcgagc acgacggcac tcggctcgag cctgccgaaa
      181 gcggactcga acgccggtcg gaggcgcagg cgggcctcgt ccacgaacag gtcgtggaac
      241 tcgcccagcc ggtcgtaccc ggacgggctc agaccaggga tgcggtcggg atcggctagg
      301 gtcatcggat ctccttcggc ggctcgcctg gggtcgacgc cgcggtggcc ggcggcgtgg
      361 ttggggacgt tgcggagggg caacgttctc atgagcctcg catacctgcg ggcaccgaag
      421 gcgagtgagt tgacaccttg ccggaggtga ccgatgctta aggccgttgt cggcctcgtg
      481 cgcccgtctg ggcgctcctg gcaggcgctc ctgccaggcc ccgccttggc cccggccgcc
      541 accaaccacc gtcctaggga cttctcggag gaactcgtgt acgatttcgt gctgacactg
      601 cataactacc tgcgctgggc gctgctcctc gtcggcctgg ccgccctggt gctcgcctgg
      661 tcgggcgccg cctcgcgcgg gcgctggacg ccgccgcagg tcaacctcgg tcgcctgttc
      721 accggcctgt tcgatctgca ggtgctcgtc ggcgtcctcc tttacgccgt gctcagcccg
      781 ctgacgaccg gcgctttcaa gaacataggc gccgccatga agaaccccga cctgcgcttc
      841 tacgtcgccg agcacctagt cggcatgctc atcgccgccg tgctcgtcca tatcggcgcg
      901 gcgcgcgcca agcgcaagga ctccccgctg caggccgcca tcttctacac cctcgccttc
      961 gtggtcgtca tcgtatccat cccctgggag cgccccctct tctagggcgt gttcgcccta
     1021 tgttgtgtgg tgctt
//
LOCUS       DAOQYE010000976         1030 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_200937_length_1030_cov_2.609231, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000976 DAOQYE010000000
VERSION     DAOQYE010000976.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_200937_length_1030_cov_2.609231"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..528)
                     /locus_tag="PKN52_12715"
     CDS             complement(<1..528)
                     /locus_tag="PKN52_12715"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNR00859.1"
                     /translation="MKTLADVSANYLTDAWHAAARTFEVVEPASGKVLAGVADCGPDE
                     AHRAADIAWSAFERWRATSAFERSDTLARWHDLIIENTEVLATTMTREMGKPLKESRG
                     EVKYAAGFVRWYAEEAKRVYGDLFPAQDPSKRLLAVPQPVGPAFAITPWNFPLAMVTR
                     NAAPALAAGCTFVLKP"
     gene            complement(677..>1030)
                     /locus_tag="PKN52_12720"
     CDS             complement(677..>1030)
                     /locus_tag="PKN52_12720"
                     /inference="COORDINATES: protein motif:HMM:NF012500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HNR00860.1"
                     /translation="HVIADGGMADLLRPALYGARHPIRVVEGQGGAAPRLPTDVDGPL
                     CENADRLGQDVELPALRAGDLLAVGTAGAYGFAMASNYAGSLRPAEVVVRGGEARLVR
                     RRERPEDLWRLEEEA"
BASE COUNT          133 a          361 c          378 g          158 t
ORIGIN      
        1 cggcttgagc acgaacgtgc agccggcggc gagcgcgggt gccgcgttgc gcgtgaccat
       61 ggccagcggg aagttccacg gggtgatggc gaaggccggg ccgacgggct gcgggacggc
      121 gagcagccgc ttgctcgggt cctgggcggg gaagaggtcg ccgtagacgc gcttggcctc
      181 ctccgcgtac cacctgacga agccggcggc gtacttgacc tcgccgcggg actccttgag
      241 gggtttgccc atctcgcgcg tcatcgtcgt ggccaagacc tcggtgttct cgatgatcag
      301 gtcgtgccag cgggcgagcg tgtcagagcg ctcgaacgcg ctcgtggccc gccacctctc
      361 gaacgccgac catgcgatgt cggcggccct gtgcgcctcg tcggggccgc agtcggcgac
      421 gccggcgagg accttgcccg atgcaggctc tacgacctcg aaggttcgcg ccgcggcgtg
      481 ccacgcgtcg gtgaggtagt tggcggagac gtcggcgagc gtcttcatgc cgccggtcgg
      541 ctttcgaggt ggaggtggct caatcccatg ctgcgagtgt agccccggcg ggtcgcgtgg
      601 agcggacccg gcgcggctac ggacgaggaa aacgttccgt cgcccggcct atggcccggt
      661 tggttggctc gaacgctcag gcttcctctt cgaggcgcca gaggtcctct gggcgttccc
      721 gcctgcgcac gaggcgggcc tcgcccccgc gcacgacgac ctcggcgggg cgcaggctcc
      781 cggcgtagtt ggaggccatg gcgaagccgt aggcgcccgc cgtcccgacc gccaacaggt
      841 cgcccgctcg cagcgcaggc aactcgacgt cctggcccaa ccggtcggcg ttctcgcaga
      901 gcggcccgtc gacgtccgtc ggcagccgcg gcgcggctcc accctggccc tctacgaccc
      961 tgatggggtg cctggcgccg tacaacgcgg gccgcagcag atcggccatg cccccgtccg
     1021 ctatcacgtg
//
LOCUS       DAOQYE010000977         1029 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_201221_length_1029_cov_3.207392, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000977 DAOQYE010000000
VERSION     DAOQYE010000977.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1029
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_201221_length_1029_cov_3.207392"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..963)
                     /locus_tag="PKN52_12725"
     CDS             complement(<1..963)
                     /locus_tag="PKN52_12725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HNR00861.1"
                     /translation="MTRRRARLLSLLGIVVAVAGLAGYWLTRDVDRKALGDDQRKTIG
                     LADEFHASFLVAGRDVVYNGTSTPVYGPGGEIVAWNFRGYHGTEGTLTDTILYVDIQD
                     DDISVIAIPRDLYLPAEAARVNSMYAYYGAQGLLERVEAILGVPIDYYVIIKLDIFQN
                     LVDALGGVVVDVPYRMDYDDNAGGLHIHFAAGPRLMSGEDAAKFVRYRHTLRGDIDRI
                     DNVKRLAYALLQKVKDLNVRAVTKVPELMDTFFSDVETNASVSIARELASRVGRLVLA
                     TTATLPNYEKEGGVPGVVYHDPVAVNAFMAATLGGEARTFQDAPD"
BASE COUNT          161 a          320 c          369 g          179 t
ORIGIN      
        1 gtcgggagcg tcctggaagg tgcgcgcctc tccgcccagc gtggcggcca tgaaggcgtt
       61 caccgccacg gggtcgtggt acaccacccc cggtacgccg ccctccttct cgtagttggg
      121 gagggtcgcc gtggtcgcga ggacgagcct gccaacacgg gaggcgagct cgcgcgcgat
      181 gctcacgctc gcgtttgtct cgacgtcact gaagaacgtg tccattagtt cgggcacctt
      241 ggtgacggcg cgcacgttga ggtccttcac cttctgcagc aaggcgtaag cgagccgctt
      301 gacgttgtct atgcggtcga tgtcgccccg cagggtatgg cgatagcgga cgaacttggc
      361 ggcgtcctcg ccgctcatga ggcgaggtcc ggccgcgaag tggatgtgga ggccgccggc
      421 gttgtcgtcg tagtccatgc ggtacggcac gtccaccacc acgcccccga gggcgtcgac
      481 gaggttctgg aagatgtcga gcttgatgat gacgtagtag tcgatgggca cgcccaggat
      541 ggcctcgacg cgctccagga ggccctgggc gccgtagtac gcgtacatcg agttgaccct
      601 cgcggcctcg gcggggaggt agaggtcgcg ggggatggcg atgaccgaga tgtcgtcgtc
      661 ttggatgtcc acgtacagga tggtgtccgt gagggtaccc tccgtgccgt ggtagccgcg
      721 gaagttccag gccacgatct cgccgcccgg tccgtagacg ggcgtggagg tgccgttgta
      781 gacgacgtcg cgaccggcga ccaggaagct cgcgtggaac tcgtctgcga gcccgatcgt
      841 cttgcgctgg tcgtcaccga gcgccttgcg atccacgtcg cgcgtcagcc agtaaccggc
      901 cagaccggcg acggccacca ctatgcctag cagcgagagc agtcgagcgc gcctacgcgt
      961 catgggcaga ccccgccgac gcgaagctgt gataggccgc caacgtggcg tggtggacgg
     1021 gcttgccgg
//
LOCUS       DAOQYE010000978         1028 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_201558_length_1028_cov_3.117163, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000978 DAOQYE010000000
VERSION     DAOQYE010000978.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1028
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_201558_length_1028_cov_3.117163"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(83..>1028)
                     /gene="fusA"
                     /locus_tag="PKN52_12730"
     CDS             complement(83..>1028)
                     /gene="fusA"
                     /locus_tag="PKN52_12730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177533.1"
                     /note="EF-G; promotes GTP-dependent translocation of the
                     ribosome during translation; many organisms have multiple
                     copies of this gene; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNR00862.1"
                     /translation="AVVGLKDTGTGDSLTDPDHPILLESIEVPEPVITVAIEPASKAD
                     QDKLSNGLIKLTEEDPTFRVESDPETGQTKISGMGELHLEIIVDRLKREFNVNANVGA
                     PQVAYRETISRPVDVEGKFVRQSGGRGQYGHVKIKAEPNPRGTGNVFENIVVGGTVPK
                     DFIPAVQKGVEEAMQSGPLLGFPIVDMKVALYDGSYHEVDSSEMSFKIAASMAVREAM
                     AKGGAQVLEPIMRVEATTPDQYMGDVIGSLNSRRGQIQGMTPRGNAQVVTANVPLAEM
                     FGYATDLRSLTQGRATFSMFFDHYEATPRSVQDELLKK"
BASE COUNT          156 a          316 c          356 g          200 t
ORIGIN      
        1 atcgtcccca cattcacatg cggcttcgta cgctcaaaaa cacccttagc catgatcaat
       61 gctccttagg taggtttcct gattacttct tgagaagctc gtcttgaacg ctgcgaggcg
      121 tggcctcgta gtgatcgaag aacatcgaga acgtggcgcg gccctgagtg aggctgcgca
      181 ggtcggtggc gtagccgaac atctcggcca gcggcacgtt ggcggtgacg acctgggcgt
      241 tgccgcgcgg ggtcatgccc tggatctggc cgcggcgcga gttcaggctg ccgatgacgt
      301 cgcccatgta ctggtccggc gtcgtggcct cgacgcgcat gatcggctcg aggacctggg
      361 cgccgccctt cgccatcgcc tcgcggaccg ccatggaggc ggcgatcttg aaggacatct
      421 ccgacgagtc gacctcgtgg tacgagccgt cgtagagcgc gaccttcatg tcgacgatcg
      481 ggaagcccag gagcggaccg ctctgcatgg cttcctcgac gcccttctgg acggccggga
      541 tgaagtcctt cggcacggtg ccgccgacga cgatgttctc gaagacgttg cccgtaccac
      601 gcgggttcgg ctcggccttg atcttgacgt ggccgtactg accgcgaccg ccggactggc
      661 gcacgaactt gccctcgacg tcgaccggac gcgagatggt ctcgcggtag gcgacctgcg
      721 gggcgccgac gttggcgttg acgttgaact cgcgcttgag gcggtcgacg atgatctcga
      781 ggtgcagctc gcccatcccg gagatcttgg tctggccggt ctcggggtcc gactcgacgc
      841 ggaaggtcgg gtcctcctcc gtcagcttga tgaggccgtt ggagagcttg tcctggtcgg
      901 ccttggaggc cggctcgatg gccaccgtga tgacgggctc cggaacctcg atggactcga
      961 ggaggatcgg gtggtcaggg tcggtgaggc tgtcgccggt gccggtgtcc ttgaggccga
     1021 ccacggcg
//
LOCUS       DAOQYE010000979         1028 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_201648_length_1028_cov_2.408016, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000979 DAOQYE010000000
VERSION     DAOQYE010000979.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1028
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_201648_length_1028_cov_2.408016"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /locus_tag="PKN52_12735"
     CDS             <1..486
                     /locus_tag="PKN52_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HNR00863.1"
                     /translation="TELKEKKHRYEDALSTARSAVEEGIVAGGGVTLIHAITAVKDLV
                     KKLDGDERTGAQILMRALEEPARQIAANAGAEGSVVVNAILNKNDGKYGYDAANDTYV
                     DDMFKAGIVDPAKVTRTALQNAASIGALLLTTEVVIADRPEKEDKTPPMPGGDMGGMD
                     F"
BASE COUNT          171 a          365 c          352 g          140 t
ORIGIN      
        1 acggagctca aggagaagaa gcaccgctac gaggacgccc tctccacggc ccgctcggcc
       61 gtcgaggaag gcatcgtcgc cggcggcggc gtcacgctca tccacgccat cacggccgtc
      121 aaggacctcg tcaagaagct cgacggtgac gagcgcactg gcgcccagat cctcatgcgc
      181 gccctcgagg agcccgctcg gcagatcgcg gccaacgccg gtgcagaagg cagcgtcgtg
      241 gtgaacgcga tcctcaacaa gaacgacggc aagtacggct acgacgcggc gaacgacacc
      301 tacgtcgacg acatgttcaa ggcaggcatc gtcgaccccg ccaaggtcac ccgtaccgcc
      361 ctccagaacg cggcctccat cggcgccctc ctcctcacga ccgaggtcgt catcgccgat
      421 cggccggaga aggaagacaa gacccccccg atgccgggcg gcgacatggg cgggatggac
      481 ttctaagaac cgctctaggc ggaagcgggc gccaggtccg ccgccgctgc gccgatcgac
      541 tcgagcacca aggcagcgag cgaggcgccc ccggttcaac cgggggcgcc tcgccttatc
      601 atgtcggctc tcgcgatgta actttgggca tgtcgagcgc agcgtcggaa ggatcgtcat
      661 gacagggccg ctgggtccag cgggcgcggg ttccggcccc gagggctctg gtccggcagg
      721 ttcccggttc ccgacgcccg ggttcgcagg gcccgggctc ccggtgtccg ggctcctggc
      781 gcccgggttc gtggcgcctt acgcctcggg acgatcgctg acgagaatac cctcgacgat
      841 cgggggcctg ctgggcgccg gagatgggtg gcgctcgacg ccgctgacgt tgcctaccgt
      901 cgccgccggc taccgcagag tggtcctcct cctgatcgac gggctcggct acgaccggct
      961 cgcggagcag ctggagaggg acgacctcgg tctcgccgcc ctgctggagc ggcacgcgcc
     1021 cggtcgga
//
LOCUS       DAOQYE010000980         1028 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_201800_length_1028_cov_1.598150, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000980 DAOQYE010000000
VERSION     DAOQYE010000980.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1028
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_201800_length_1028_cov_1.598150"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="PKN52_12740"
     CDS             <1..304
                     /locus_tag="PKN52_12740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00864.1"
                     /translation="SQPWQISVAMATFFGPSALTDQGWDSPETRALIQEASQIADQDR
                     RAELYAQVVDTVADQAISIPMAHNRSLNATRVGIEGWIASPLGFSSVSLHSVTKAQ"
     gene            513..>1028
                     /locus_tag="PKN52_12745"
     CDS             513..>1028
                     /locus_tag="PKN52_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNR00865.1"
                     /translation="MTAYIVRRLLGLVPVLIGVTLLIFFLTRIIPGDPAIAMLGERAA
                     PNLVERLRADLGLDRPLFFNAAEYRETGKLGDLFDSQYFDYMGGMLRGDLGRSILTLV
                     PITQSLLHRFPATFELTLAAMLLAVVIGIPAGMWAALRRGTLADTAVMLVAIAGVSFP
                     VFWLGIILIYVF"
BASE COUNT          151 a          382 c          314 g          181 t
ORIGIN      
        1 cagccagccc tggcagatct cggtcgccat ggcgacgttc ttcggcccct cggccctcac
       61 ggaccaaggc tgggatagcc cggagacccg cgccctcatc caggaggcca gccagatcgc
      121 cgaccaggac cgccgcgccg agctctacgc tcaggtcgtc gacacggtgg ccgatcaggc
      181 catctcgatc ccgatggcac acaaccgctc gctgaacgcg acgcgcgtcg gcatcgaagg
      241 ctggatcgcg agcccgctcg gcttcagctc cgtcagcctg cactccgtca cgaaggcgca
      301 gtaagaccaa gcggtaagca cgagatcaca gggttcggga gcgcctgacc gcgctcccga
      361 accgcctgcc gggagtgctg gggacgccct gcatggggcg cccccagccg gtccgggtta
      421 tgttgctgcg agaatcactt atcgacccgg aggcgctggg cgcactacca agctagcgcg
      481 cccgttcgcc gggtcctatt aggagcaagc gcttgaccgc gtatatcgtc cgccgcctgt
      541 tgggtctggt ccccgtcctc atcggggtga ccctgctcat cttcttcctc acgcgcatca
      601 tccctggcga cccggcgatc gccatgctcg gggagcgcgc ggctccgaac ctcgtggagc
      661 ggctccgcgc cgacctgggg ctcgaccggc cgttgttctt caacgcggcc gagtaccgcg
      721 agacgggcaa gctcggcgat ctcttcgact ctcagtactt cgactacatg ggcggcatgc
      781 tgcgcggcga cctcggccgc tccatcctca cgctcgtacc gatcacccaa agcctcctgc
      841 accgcttccc cgcgacgttc gagctcacgc ttgcggccat gctgctcgcc gtcgtcatcg
      901 gcatccctgc aggcatgtgg gcggcgttgc ggcgcggcac cctggccgac acggccgtga
      961 tgctggtggc catcgccggc gtgtcgttcc ccgtcttctg gctcggcatc atcctcatct
     1021 acgtcttc
//
LOCUS       DAOQYE010000981         1027 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_201949_length_1027_cov_2.454733, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000981 DAOQYE010000000
VERSION     DAOQYE010000981.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1027
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_201949_length_1027_cov_2.454733"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1027)
                     /locus_tag="PKN52_12750"
     CDS             complement(<1..>1027)
                     /locus_tag="PKN52_12750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00866.1"
                     /translation="VGLVGAYTNHYEEDCLNFGADVIGKIPGLDATLTLAGAGAWVKT
                     DTWKFEGAEDNAAFLAKISDLKLSPVTAWASYTGVGGNFFAHYKSTAGTAILNKYAGS
                     AAAEVGIAAELPVGIPATLTLGDTLWMGYPTDTKWNETTGKVVVTPMENLDVTVSGAY
                     KADLDDADAKDYKGYKAHADVKYTAFGLELNPFVDYKVDSYADATVHTGGKVVDTIVG
                     LDIYGSPLAGLDVGVEASYTVEEPVTALKGWGIYATELNPGFVKSAMTKIAGVAEYNK
                     EASDPATTDFYGYAGSDIVVTDKLTAKVGVLTKDEKKEIAAHAGLTYKVSDSIATGLA
                     YTYRAFAV"
BASE COUNT          182 a          317 c          301 g          227 t
ORIGIN      
        1 ccaccgcgaa agcgcggtag gtgtaggcca gaccggtcgc gatgctatcg ctcaccttgt
       61 aggtcaggcc agcgtgtgcc gcgatctcct tcttctcgtc cttcgtcagc acgccaacct
      121 tggcggtcag cttgtcggtg acgacgatgt ccgaaccggc atagccgtag aagtcggtgg
      181 tcgcagggtc actggcctcc ttgttgtact ccgccactcc ggctatcttg gtcatggcgc
      241 tcttcacgaa accagggttc agctcagtgg cgtagatgcc ccagcccttg agagccgtca
      301 cgggctcctc aacggtgtag cttgcctcga cgcccacgtc cagtcccgcc agcgggctgc
      361 cgtagatatc gagaccaacg atggtgtcga caaccttacc gcctgtgtgc actgtcgcat
      421 ctgcgtagga gtcgaccttg taatcgacga acgggttgag ttccaggcca aaagcggtgt
      481 acttgacatc cgcatgagcc ttgtagccct tgtagtcctt tgcgtccgcg tcatcgaggt
      541 ctgccttgta ggcgcccgag acggtcacgt caaggttctc catgggagtg acgactacct
      601 taccggtcgt ctcattccac ttagtatctg tagggtagcc catccacagg gtatcgccca
      661 gcgtcagcgt agccggaatg ccgactggaa gttccgccgc gatgccgact tcagccgccg
      721 cactgccagc gtacttgttg aggatcgcag tccctgccgt ggacttgtaa tgagcgaaga
      781 agttgccgcc aacaccggtg tagcttgccc aggcggtgac ggggctcaac ttcaggtcgc
      841 tgatcttggc gaggaaggcg gcgttgtctt cagcgccttc gaacttccag gtatccgtct
      901 tcacccaggc tccggcgccg gccagagtaa gggtggcgtc caggccaggg atcttgccga
      961 tgacatcggc tccgaagttc aggcaatcct cctcatagtg gttggtgtat gctccaacca
     1021 gtccgac
//
LOCUS       DAOQYE010000982         1027 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_201951_length_1027_cov_2.445473, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000982 DAOQYE010000000
VERSION     DAOQYE010000982.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1027
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_201951_length_1027_cov_2.445473"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..604)
                     /locus_tag="PKN52_12755"
     CDS             complement(<1..604)
                     /locus_tag="PKN52_12755"
                     /inference="COORDINATES: protein motif:HMM:NF016240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HNR00867.1"
                     /translation="MALTDIELRVLGSLVEKERTTPDGYPLTMQALVTACNQKTSRDP
                     VTDYHLQDVQDALQRLRDKGLAATTQEVSDRVPKHRHKIAQAWSVNDKEIALLGVLML
                     RGPQTPGELRTRTERYVAFADVADVERVLDGLAGRSVPLAANLGRGPGQSQDRWTHKL
                     GADEERMQPRVRHGGAAAGGDHGAAPGGTGAAAATGGSPLA"
     gene            668..>1027
                     /locus_tag="PKN52_12760"
     CDS             668..>1027
                     /locus_tag="PKN52_12760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00868.1"
                     /translation="MPIRDELIERTIQQLAALMAKLIGARFGVLEAARVDLGAAEREL
                     ERLYLSNLGTSRGLVHRLGLEDLIGVLSTAGYVDAERAYLLGALLSVDAEVAVAAGAD
                     ADDPEVVGLRSAALAALL"
BASE COUNT          118 a          370 c          390 g          149 t
ORIGIN      
        1 ccgccagcgg cgagccgccg gtcgcggccg ccgcaccggt cccgccaggt gcggcaccat
       61 ggtcgccgcc tgccgccgcg ccgccgtgac gcacgcgggg ctgcatgcgc tcctcgtccg
      121 cgcccagctt gtgggtccag cggtcctggc tctggcccgg gccgcggccg aggttcgccg
      181 ccagcgggac ggagcggccg gccaggccgt cgaggacgcg ctcgacgtcc gccacgtcgg
      241 cgaaggcgac gtagcgctcc gtgcgcgtgc gcagctcgcc gggcgtctgg gggccacgca
      301 gcatgaggac gccgagcagc gcgatctcct tgtcgttcac cgaccaggcc tgcgcgatct
      361 tgtggcggtg cttggggacg cggtcgctga cctcctgcgt cgtcgcggcg agtcccttgt
      421 cgcggaggcg ctgcagggcg tcctgcacgt cctgcaggtg gtagtcggtg acggggtcgc
      481 ggctggtctt ctggttgcag gcggtgacga gcgcctgcat cgtgaggggg taaccgtccg
      541 gcgtcgtgcg ctccttctca acgagggagc ccagtacgcg cagctcgatg tcggtgaggg
      601 ccatgcgggc caggatatta cgcgtgcggc cggcccggcc caagccccgg tgcgatagcc
      661 tggcgccgtg cccatccgtg acgaactgat cgaacgaacc atccaacagc tggccgccct
      721 catggccaag ctcatcggcg cgcgcttcgg cgtgctcgag gcggcgcgcg tcgacctggg
      781 agcggccgag cgcgagctcg agaggctcta cctctccaac ctaggcacga gccgcggcct
      841 cgtgcaccgc ctgggcctcg aggacctgat cggggtcctg agcacggccg gctacgtcga
      901 cgccgagcgg gcgtacctcc tgggcgccct cctcagcgtc gacgcggagg tggcggtggc
      961 ggcgggcgcc gacgcggacg acccggaggt cgtggggctc cgctccgccg cgctggcggc
     1021 gctgctc
//
LOCUS       DAOQYE010000983         1027 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_202072_length_1027_cov_1.862140, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000983 DAOQYE010000000
VERSION     DAOQYE010000983.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1027
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_202072_length_1027_cov_1.862140"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            164..>1027
                     /locus_tag="PKN52_12765"
     CDS             164..>1027
                     /locus_tag="PKN52_12765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00869.1"
                     /translation="MPTESASVRWLLRGDPAIRWQTMRDLVGAPAAEYERERATVATN
                     GWGAESLARQDEDGQWRVERAEWPLAPTLYSLQLLRHCGLDPLSEQARRAIARVREGV
                     RWEFWGNAPFFDGEVEPCINGGVLAVGAYFGVASSKLLDRLLGEQLADGGWNCEAEQG
                     SVRSSFHTTICVLEGLLEYERSMGPAPVVTEARLRAEEYLLERRLLRRASTGKIIDTY
                     WTRFPFPTYWRYDVLRALEHLRAAGRERDPRLSEPLDIVRRRRRQDGRWSLGEPYGFD
                     HHGVALDPGRGK"
BASE COUNT          139 a          353 c          377 g          158 t
ORIGIN      
        1 ctgatcctgg tggccatgct cgtagccgag ctgaggctgc ctgttccggc ccgtgaccgg
       61 ctgaccactc ccgacaggtg atcacttcgc ccaccccgat cgcgcctacg ctccggccgg
      121 cccgcggttg acccggaggg ctcccggtct ctatcgttcc tccatgccca ccgagagcgc
      181 ctccgttcgc tggctcctgc gcggcgaccc ggccatccgc tggcagacca tgcgcgacct
      241 ggttggcgcc cccgccgccg agtacgagcg ggagcgcgcg accgtcgcca cgaacggctg
      301 gggcgcagag tcgctggcgc gccaggacga ggacggccag tggcgcgtcg agagggcgga
      361 gtggccgctg gcgccaacgc tctactcgct gcagctcctg cggcactgcg ggctcgatcc
      421 gctgagcgaa caggccaggc gcgccatcgc gagggtgcgg gagggcgtgc gctgggagtt
      481 ctggggcaac gcccccttct tcgacggcga ggtggagccg tgcataaacg gcggcgtgct
      541 ggcggttggc gcatacttcg gggtggcctc ctccaagctg ctcgaccgcc tgctgggaga
      601 gcagctcgcc gacggcggct ggaactgcga ggcggagcaa ggttcggtcc gctcgtcgtt
      661 ccacacgacg atctgcgtct tggagggcct gctcgagtac gagaggtcga tggggcccgc
      721 gcccgtcgtg accgaggcga ggttgcgcgc cgaggagtac ctgctggagc ggcggttgct
      781 gaggcgtgcc agcacgggca agatcatcga tacgtactgg acgcgcttcc cgttccccac
      841 ctactggcgt tacgacgtac tgcgcgccct ggagcacctg cgggccgctg ggcgcgagcg
      901 cgatccgcgc ctgagcgaac cgctcgacat cgtcaggcgc cggcgtaggc aggacggccg
      961 gtggtcgctg ggtgagccgt acggcttcga ccaccacggc gtggcgctcg atcccggtcg
     1021 cgggaag
//
LOCUS       DAOQYE010000984         1027 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_202169_length_1027_cov_1.198560, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000984 DAOQYE010000000
VERSION     DAOQYE010000984.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1027
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_202169_length_1027_cov_1.198560"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="PKN52_12770"
     CDS             complement(<1..314)
                     /locus_tag="PKN52_12770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00870.1"
                     /translation="MPARAPIPELMSPAGHWPQLRAAVEAGADAVYFGLSRFSARSKV
                     GFTTEELPDAVMELHARAVRAYVTFNTLVFDSELADARRALEAIGAAGADALIVQDVA
                     AAA"
     gene            387..905
                     /locus_tag="PKN52_12775"
     CDS             387..905
                     /locus_tag="PKN52_12775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNR00871.1"
                     /translation="MIAGARYAHTNLVARDWRALADFYVRLFGCEFVPPERDDAGPAL
                     EAGTGVPGAAMAGVHLRLPGHGPGGPTLEIFTYTAAVDAMAAVVNRPGYGHIAFEVAS
                     VADARRTRTSPASTAQEPCCRPSPAATSSRRSWSWRSSNWRSRRTGLPLMPSWRRSSK
                     AKGGTGERPEGG"
BASE COUNT          142 a          364 c          388 g          133 t
ORIGIN      
        1 gcggcggcgg cgacgtcctg cacgatgagc gcgtccgcgc cggcggcgcc gatggcctcg
       61 agcgcgcgcc gggcgtcggc gagctccgag tcgaagacga gcgtgttgaa cgtcacgtag
      121 gcgcgcacgg cgcgcgcgtg gagctccatc accgcgtcgg gcagctcctc cgtggtgaac
      181 ccgaccttgg agcgggcgct gaagcgcgag aggccgaagt agacggcgtc cgcgcccgcc
      241 tcgaccgccg cccgcaactg gggccagtga ccggcggggc tcatgagttc cgggatgggg
      301 gcgcgtgctg gcatggcgag ggcgatgcta gcagtaggcg cggcggtggg acgtggcggc
      361 ggtcggcctc gccgtaagtt gaccccgtga tcgccggcgc ccgctacgcc cacaccaacc
      421 tggtggcccg cgactggcgg gccctcgcgg acttctacgt ccggctcttc ggctgcgagt
      481 tcgtgccgcc cgagcgcgac gacgctggcc ccgccctcga ggccggcacg ggcgtgccgg
      541 gcgccgccat ggccggcgtc cacctgcgcc tgccggggca cgggccagga gggccgaccc
      601 tcgagatctt cacctacacg gcggcggtcg acgctatggc ggccgtagtg aaccggcccg
      661 ggtacgggca catcgccttc gaggttgcct cggtggcgga cgcccgccgg acgcggacca
      721 gcccggcctc gacggcgcaa gaaccctgct gcaggccgtc accggcagct acatcctcgc
      781 gccgatcctg gtcctggcgg tcatccaatt ggcgaagccg tcgaaccggg ttgcccctga
      841 tgcccagttg gcggcgatcg agcaaggcta aaggtggtac gggagaacga cccgagggcg
      901 gctgacaaca gggctcccgg cggcaagcat cggccttcga gcccgattgc ccgccatcac
      961 ccggcgcgca ggactcgcga ccggcttaac gggcgtgtcg atagcgcgtc gggtcgggca
     1021 ggccggc
//
LOCUS       DAOQYE010000985         1025 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_202590_length_1025_cov_2.827835, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000985 DAOQYE010000000
VERSION     DAOQYE010000985.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_202590_length_1025_cov_2.827835"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            345..983
                     /locus_tag="PKN52_12780"
     CDS             345..983
                     /locus_tag="PKN52_12780"
                     /inference="COORDINATES: protein motif:HMM:NF019352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HNR00872.1"
                     /translation="MRDSRGNVPIGAIYGLVALFVALAVGVALFGSRVDAATATETAQ
                     AAHGTHELTVNMPSARKVFLDDAEDLTPVSELPVLKGVLGYAPNVAPPVDRDYRAHVE
                     IELEVTEEVMRLADGVEYRFWTFGGSVPGPMIRVRQGDYVTFTLQNHPTSLMPHNIDL
                     HGVTGQGGGAEATLISPGQQATFSFTALQAGVYIYHCATAPVGMHIATACTA"
BASE COUNT          177 a          357 c          328 g          163 t
ORIGIN      
        1 cgcgatcttc gctgcagccg cgcccgggtt ctcggcggcg tagattagcg cgtggatcgc
       61 gtagctctcc tcgcggcgca ggagggtccg gtacggtgag agatcgactg gctcggccac
      121 acacccaggg tagcaggtga ctaggacgtg agtgtcctga tatgcgcgct gcttcggtcg
      181 ccccgtggga cagtgcggtt aaacggttta ccggacgcga cggcgcagga catctgtcct
      241 tggatgtccg ccccccaccg cattaatttg gactcagtta tccgggtttg gcggcgagcc
      301 taagtcgtcg aacgtgaaga gaccggagcc caggaggaaa ggccatgcga gactcacgcg
      361 gtaacgtccc aatcggagcc atctacgggc tggtagccct cttcgtggcg ctcgccgtcg
      421 gcgtggccct gttcgggagc cgcgtcgacg ctgcgacggc aacggagacc gcgcaggccg
      481 cccacggaac tcacgaactg accgtcaaca tgcccagcgc caggaaggtc ttcctcgacg
      541 acgccgagga cctgaccccc gtctcagagc tgcccgtcct caagggcgtg ctgggctacg
      601 cgcccaacgt cgcgccgccc gtcgaccgcg actaccgcgc ccacgtcgag atcgagctcg
      661 aggtcacgga agaggtcatg cgcttggccg acggcgtcga gtaccgcttc tggacgttcg
      721 gcggctccgt cccgggaccc atgatccggg tcaggcaggg cgactacgtc acgttcaccc
      781 tccagaacca ccccactagc ctcatgccgc acaacatcga cctccacggc gtgacgggcc
      841 agggcggcgg cgcggaagcc accctcatct cccccggcca gcaggcgacg ttctccttca
      901 cggccctcca ggcgggcgtg tacatctacc actgcgcgac tgcgccggtc ggcatgcaca
      961 tcgcaacggc atgtacggcc tgatcgtcgt cgagcccaag gagggctacg cccccgtcga
     1021 caagg
//
LOCUS       DAOQYE010000986         1024 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_203146_length_1024_cov_1.775026, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000986 DAOQYE010000000
VERSION     DAOQYE010000986.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_203146_length_1024_cov_1.775026"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            286..>1024
                     /locus_tag="PKN52_12785"
     CDS             286..>1024
                     /locus_tag="PKN52_12785"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNR00873.1"
                     /translation="MRSNRLVPYLFLLPALAILALGLMVPLYNAVLLSFYDWNFGQAW
                     SDARYLGLANFTRLWSDPTVWSSVRTTFVFGFWVVALEMIIGIGLALLLEKSMRGATF
                     FRTILVMPLMIAPVVVGLIWRYLLDARNGVINYYLDSIGQAVPFLQGLGFKSHAWLGD
                     PNLAMVSIIVSDVWQWTPFVFMIVLAGLQGLPGEVMEAAYIDGANWLRMTWHVKLPML
                     RNILLIALLMRVIDVFRALEVIYVMTGG"
BASE COUNT          162 a          332 c          324 g          206 t
ORIGIN      
        1 cgccatgaac gccccgatcc tgggcgagga gctgtccgct gcgctgaccg gacagaagtc
       61 catcgaggac gccctcgaga acgcctccaa gcgcatctac gagctgctcg accgcgccgg
      121 gtactacacc tggtgagttg agcgccgatg gggtgccacg gcggcgttgc ccggccgtgg
      181 cacccgaacc cccggccggg cgccgcccag ggcgtcccgg acgaagggcg aacgactatc
      241 ttccgtagtc gaccaccgtt gcgagctaga gagaggaggt agccgatgcg atcgaaccgc
      301 ctggtcccat atcttttcct cttgccggcg ctggccatcc tggccctggg gctgatggtt
      361 cccctataca acgcggttct tctcagcttc tacgactgga acttcggcca ggcctggagc
      421 gacgcgcgtt acctgggtct tgccaacttc acgcgtctct ggagcgaccc gaccgtctgg
      481 agctcggtgc gaacgacgtt cgtgttcggg ttctgggtgg tggccctcga gatgatcatc
      541 gggatcggcc tggcgctact gctcgagaag tccatgcgag gcgcgacctt cttccggacg
      601 atcctcgtca tgccgttgat gatcgcaccg gtggtcgttg gcctcatctg gcgctacctc
      661 ctcgacgcca ggaacggcgt aatcaactac tacctcgatt cgattggtca ggccgtcccg
      721 ttccttcagg gcctgggctt caagagtcac gcctggctcg gggacccgaa cctcgccatg
      781 gtctcgatca tcgtgagtga cgtctggcag tggaccccct tcgtcttcat gatcgtgctc
      841 gccgggctcc agggcctacc gggcgaggtc atggaggccg cgtacatcga cggcgccaac
      901 tggctccgca tgacgtggca cgtgaagctg ccgatgctga ggaacatctt gctgatcgcc
      961 ctgctcatgc gcgtgataga cgtgttccgc gcgctcgagg tgatctacgt tatgaccggg
     1021 ggtg
//
LOCUS       DAOQYE010000987         1021 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_204120_length_1021_cov_2.061077, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000987 DAOQYE010000000
VERSION     DAOQYE010000987.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_204120_length_1021_cov_2.061077"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            171..545
                     /locus_tag="PKN52_12790"
     CDS             171..545
                     /locus_tag="PKN52_12790"
                     /inference="COORDINATES: protein motif:HMM:TIGR01550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HNR00874.1"
                     /translation="MTEYLTVAEVLAIHEDQVQRYGGESGVRDLGSLEAALFRPQTGY
                     YPTVIEEATALWESLAQNHPFVDGNKRTAFAATYTFLAINGQRITASAGATYDFIHQL
                     YERGAFRFDRLVRWLSANTEQF"
     gene            666..>1021
                     /locus_tag="PKN52_12795"
     CDS             666..>1021
                     /locus_tag="PKN52_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HNR00875.1"
                     /translation="MKAFLRVAGFIDAFNGAIAVVMQWLTLVMVLLGAYNVITRYVGR
                     AIGVSLGGSVYTVLQTYAFDLVFLLAAGWVLRTEGHVRVDIVYSRLRERGKAWVDLLG
                     TVFFLFPFCYMGFTLSL"
BASE COUNT          171 a          332 c          335 g          183 t
ORIGIN      
        1 gatatccggg ccctcgcgcg cagcgagggc aggcagatcc aagcactggt cgacgaggcg
       61 ctcgccgacc tgctcgagaa gcggcggcag gagaagcctc gtgcgcacgt catggccgcc
      121 tatcaggtga gccacgagaa gttcgcggcg ctctacaaga agctggctga atgaccgagt
      181 acctcacggt cgccgaggtc ctggcgatac atgaggatca ggttcagcgt tacgggggcg
      241 agagcggcgt tcgcgacctg ggttcgctcg aagccgctct gttccgccca cagaccggct
      301 actacccaac cgtgatcgag gaagccaccg cgctctggga gagcttggca cagaaccacc
      361 ccttcgtgga cgggaacaag cggaccgcct tcgcggccac ctacacgttc ttggccatca
      421 acggccagcg gatcacggct tcggccggag ccacgtacga cttcatccac caactgtacg
      481 agcgtggggc gttccgattc gaccggctcg tccgctggct gtcagccaac accgaacagt
      541 tctgagcgac gggcagcctc cctgcttgct ccacgccggc gcggcgttga cgctcccagg
      601 tgacggcagt accatgaccc ccagcaacca agagtgatga gcggtcacgg aggggaggcg
      661 cctggttgaa ggcgttcttg agggttgccg ggttcatcga cgcgttcaac ggcgccatcg
      721 cggtcgtcat gcagtggctc acgttggtga tggtgctcct cggcgcctat aacgtcatca
      781 cgcgctacgt ggggcgcgct atcggggtct cgctcggtgg cagcgtctac acggtgctgc
      841 agacgtacgc cttcgacctg gtgttcctgc tcgccgccgg ctgggtgctg cgcacggagg
      901 ggcacgtgcg ggtcgacatc gtctactcgc gcctgcggga acgcggcaag gcgtgggtcg
      961 acctgctcgg cacggtcttc ttcctcttcc cgttctgcta catgggcttc acgctcagcc
     1021 t
//
LOCUS       DAOQYE010000988         1020 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_204510_length_1020_cov_2.132642, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000988 DAOQYE010000000
VERSION     DAOQYE010000988.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_204510_length_1020_cov_2.132642"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(6..>1020)
                     /locus_tag="PKN52_12800"
     CDS             complement(6..>1020)
                     /locus_tag="PKN52_12800"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing phosphodiesterase"
                     /protein_id="HNR00876.1"
                     /translation="VTPFELHGLSHHVSASIGICLYPDDAPDGATLLRHADSAMYRVK
                     RSGKNGIQRYRDDSDAVVERQRNLERELKTALSTGREQLSLVYQGIHDLETTELKKVE
                     ALARWHHPVWGHVPPHEFIPIAERSGLIVTLGEWVLREACRQAKEWEALSGRGIVVSV
                     NVSAMQFAHPGFFRTVTDALEDHALQPELLEIELTEGIVMHGVDHVTETLKRLQRLGV
                     SIAIDDFGTGYSSFAYLRDLPIDTVKIDRSFVKDLGAPLHAPQFALALVEAITHVAKH
                     LDLKIVAEGIETEAQRQVLRDLGCHLGQGYLFSKPVPRSELRAFMHLNPTEERLLGES
                     FIN"
BASE COUNT          166 a          320 c          349 g          185 t
ORIGIN      
        1 tcgggtcagt tgatgaagct ctcgcccagc aggcgctcct cggtcgggtt gaggtgcatg
       61 aaggccctca gctcggagcg cggcacgggc ttggagaaca ggtagccctg acccaggtgg
      121 cagccgagat cacgtagcac ctggcgttgc gcctccgtct cgatcccttc cgccacgatc
      181 ttcagatcaa ggtgcttggc cacgtgggtg atggcctcca ccagggcgag agcgaactgc
      241 ggggcgtgga gtggcgcgcc gaggtccttg acgaagctgc gatcgatctt cacggtgtcg
      301 ataggcaggt cgcgaaggta agcgaaggag gagtaccccg tgccgaagtc gtctatcgcg
      361 atgctcacgc cgaggcgctg cagacgcttg agcgtctccg ttacatgatc gacaccgtgc
      421 atgacgatgc cctccgtcaa ctcgatctcg agcagttcag gttgcagcgc atggtcctcc
      481 agcgcgtccg tcacggtgcg gaagaagccc gggtgcgcga actgcatggc cgagacgttg
      541 acggagacga cgatgcctcg tcctgagagc gcctcccact ccttggcctg ccggcacgcc
      601 tcgcgcagga cccactcgcc gagcgtaacg atcaggccgc tgcgctcggc gatggggatg
      661 aactcgtgag gcggcacatg gccccagacc gggtggtgcc aacgcgccag cgcctcgacc
      721 ttcttcagtt cggtcgtctc caggtcgtgg atgccctggt acacgagcga cagctgctcc
      781 ctgccggtgc tgagcgccgt cttcagctca cgttcgaggt tgcgctggcg ttcgacgacc
      841 gcgtcggagt cgtcgcggta gcgctggatg ccgttcttgc ctgaacgttt gacgcggtac
      901 atggcggagt cggcatggcg cagcagcgtc gcgccgtccg gcgcgtcgtc ggggtagagg
      961 cagatgccga tgctcgcgct cacatggtgc gagagcccgt ggagctcgaa cggcgtcact
//
LOCUS       DAOQYE010000989         1020 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_204555_length_1020_cov_1.902591, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000989 DAOQYE010000000
VERSION     DAOQYE010000989.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_204555_length_1020_cov_1.902591"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(8..517)
                     /locus_tag="PKN52_12805"
     CDS             complement(8..517)
                     /locus_tag="PKN52_12805"
                     /inference="COORDINATES: protein motif:HMM:NF013440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HNR00877.1"
                     /translation="MSRAFVKEDASGEDVVVTHRAPLPDGVPNLVTPAGLAALERERD
                     ERRHELEVLMDSEPTPDGRRKLAAAEEELELLLGRVATAQVVGPPPDRDEAGVGATVT
                     VRYLSGPQAGRTAEFTVVGVDEADPLEGLVAFTAPVAQALLGQRAGAEGAFMAGDTEL
                     RVRLERVSY"
     gene            complement(514..>1020)
                     /locus_tag="PKN52_12810"
     CDS             complement(514..>1020)
                     /locus_tag="PKN52_12810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00878.1"
                     /translation="PLLTDTSYRFGVSVNGGTSADVPSQLGCDDGSEACLLEVVFERT
                     DLRTMGVSGTSTDTLVYYLQNAELSAADRAKLEQLADLKGQYATLQRESDAIANRAKE
                     IDAEQSRVRQNMAQLDRNSTLYRRYVDDLTAQEDELAASTERREEVRAALVDLQEQID
                     SLIASLGQ"
BASE COUNT          132 a          362 c          358 g          168 t
ORIGIN      
        1 acagacccta gtagctcacg cgctcgagcc gcacccgcaa ctcggtgtcg cctgccatga
       61 acgccccctc ggcgccggcg cgctggccga gcagcgcctg cgcgaccggc gccgtgaacg
      121 ctaccaggcc ctcgagcggg tcggcctcgt cgaccccgac cacggtgaac tcggcggtgc
      181 gcccggcctg ggggccgctg aggtagcgga ccgtcaccgt cgcgccgacg cccgcctcgt
      241 cgcggtcggg cggtgggccc acgacctggg cggtggcgac gcggccgagc aggagctcca
      301 actcctcctc ggccgcggcc agcttgcgcc tgccgtccgg tgtgggctca gagtccatga
      361 ggacctcgag ctcatgcctg cgttcgtcgc gctcacgttc cagcgccgca agccctgccg
      421 gggtgacgag gttcgggacg ccgtccggca gtggcgcgcg gtgggttacc accacgtcct
      481 cgccggaggc gtcctccttg acgaacgccc tgctcactgc cccaagctgg cgatgaggga
      541 gtcgatctgc tcctgcaggt ccaccagcgc cgcgcgaacc tcctcgcggc gttcggtcga
      601 ggcggccagc tcgtcctcct gcgccgtgag gtcgtctacg tagcggcggt acaaggtcga
      661 gttgcgatcc aattgggcca tgttctggcg gacgcgcgac tgctcggcgt cgatctcctt
      721 ggcacggttc gcgatggcgt cgctctcacg ctgcagcgtg gcgtactggc ctttgaggtc
      781 cgcgagctgc tcgagcttgg cccggtcggc ggcgctaagc tcggcgttct ggaggtagta
      841 gacgagggtg tcggtcgacg tgccgctcac gcccatcgtg cgcaggtcgg tacgctcgaa
      901 cacgacctcg agcagacaag cttccgagcc gtcgtcgcag cccagctgcg atggcacgtc
      961 ggcgctggtg cctccgttga cggagacgcc gaagcggtag ctcgtgtcgg tcagtagcgg
//
LOCUS       DAOQYE010000990         1017 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_205671_length_1017_cov_1.569647, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000990 DAOQYE010000000
VERSION     DAOQYE010000990.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_205671_length_1017_cov_1.569647"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(82..489)
                     /locus_tag="PKN52_12815"
     CDS             complement(82..489)
                     /locus_tag="PKN52_12815"
                     /inference="COORDINATES: protein motif:HMM:NF019886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HNR00879.1"
                     /translation="MIPVFDGHNDTLLNHIEDPKRDFFSRQADGHVDYVRAREGGMVG
                     GFFAEGLLLEREAAGDFLLATPSGSPQLNGVFYSPDDRAVITLVELEDYSMEDAARWL
                     DCGLSAVKVRAFRARRRLKVVLEALRPDKEPAL"
     gene            complement(624..>1017)
                     /locus_tag="PKN52_12820"
     CDS             complement(624..>1017)
                     /locus_tag="PKN52_12820"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNR00880.1"
                     /translation="AIVGIPSYARIVRSVVIAAREEDFVVAAKALGARDPRLLLKHVL
                     PSALGALSVQATLGIGFAILSMAGLSFLGLGVQPPTADWGEMLARGRRYLPGSPWLLL
                     YPGAAISLTVLAFNLLGDGLRDALDPRD"
BASE COUNT          158 a          327 c          399 g          133 t
ORIGIN      
        1 tgatggccgc cgtatcgagg gccggggccg ccgggcggta ggccgcgcgg agctggtccc
       61 aggggtgtcg cggggcggtg ttcataacgc gggttccttg tccggccgta gggcttcgag
      121 cacgaccttc aagcggcgtc gcgcacggaa cgcgcgcacc ttgacggcgg acagcccgca
      181 gtccagccag cgcgcggcgt cctccatgga atagtcttcc aactcgacga gcgtgatgac
      241 cgcgcggtcg tcgggcgagt agaagacgcc gttcagctgc gggctgccgg atggcgtcgc
      301 gaggaggaag tcgccggcgg cctcgcgctc gagcagcagt ccctcggcga agaagccgcc
      361 gaccatgccg ccctcgcgcg cgcggacgta gtcgacgtgg ccgtcggcct ggcgcgagaa
      421 gaagtcgcgc ttcgggtctt cgatgtggtt gaggagggtg tcgttgtggc catcgaacac
      481 tgggatcatg gccgcaaggc taccgcttcg cgggccgtac ggtcactggc gcccgaccca
      541 accccggacg gaactgtccg cgtgacgccg aagccggcgc ctgacccggc accctgagcc
      601 ggctcaggta gctggcgctc gactcagtcg cgcggatcca gcgcgtcgcg gaggccgtcg
      661 ccgagcaggt tgaaggcgag cacggtcagc gagatggcgg cgcctggata gaggaggagc
      721 cagggggagc cggggaggta gcggcgtccg cgggcgagca tctcgcccca gtcggcggtg
      781 ggcggctgca cgccgaggcc gaggaacgac aggccggcca tggagaggat ggcgaagccg
      841 atcccgagcg tcgcctgcac gctcaaggcg ccgagcgcgc tcggcagaac gtgcttgagg
      901 aggaggcgcg ggtcgcgggc gccgagcgcc ttggccgcga cgacgaagtc ctcctcgcgc
      961 gcggcgatga cgacgctgcg cacgatgcgc gcgtaagacg gtatgcccac gatggcg
//
LOCUS       DAOQYE010000991         1016 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_205791_length_1016_cov_3.113424, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000991 DAOQYE010000000
VERSION     DAOQYE010000991.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1016
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_205791_length_1016_cov_3.113424"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="PKN52_12825"
     CDS             <1..454
                     /locus_tag="PKN52_12825"
                     /inference="COORDINATES: protein motif:HMM:NF015649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bacterial transcriptional activator
                     domain-containing protein"
                     /protein_id="HNR00881.1"
                     /translation="LGDGETIIASDPQAIVAQAREAAVPRQIDLLSQAVGMVRGEILA
                     GFDYAWVAPLRMRLTESVANALHRLGELLLATGQAQEARGVAVRYTQFDPLDERAHLL
                     FMRAQKALGDLVGVEQTHRRLVELLRSELGVQPTAETERQYRDLLGRS"
     gene            complement(449..>1016)
                     /locus_tag="PKN52_12830"
     CDS             complement(449..>1016)
                     /locus_tag="PKN52_12830"
                     /inference="COORDINATES: protein motif:HMM:NF017024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HNR00882.1"
                     /translation="VAKANVLAALRHGAYVVVGTSGLTERDYAEIDAAAGQAGRGVLA
                     CGNFALTVVLLLKFAEAAARHIPHWEIIDYASAGKPDAPSGTARELAARLGRAATPQL
                     ETPVSATVGERAARGATLGGAQVHSVRLPGYTIGVEAIFGLPDQHLSIRHDAGPSSEP
                     YVDGAILAIRKVGALTGLHRGLDTVLEL"
BASE COUNT          136 a          347 c          379 g          154 t
ORIGIN      
        1 gctgggggac ggggagacca tcatcgcgtc ggacccgcag gccatcgtgg cccaggcccg
       61 cgaggcggcc gtgcccaggc agatcgactt gctcagccag gcggtcggca tggtgcgggg
      121 cgagatcctc gcggggttcg actacgcgtg ggtcgcaccc ctgcgcatgc gcctgaccga
      181 gtcggtcgcc aacgccctgc ataggctcgg cgaactcctg ctggccacgg ggcaggcgca
      241 ggaggcgcgc ggcgtagccg tgcgctatac gcagttcgac ccgctcgacg agcgcgctca
      301 cctcctcttc atgcgcgcgc agaaggcgct cggtgatctg gtcggcgtgg agcagacgca
      361 ccggcggttg gtcgagctgc tcaggtccga gttgggcgtg cagccgaccg ccgagacgga
      421 gcggcagtac cgggacctgc ttgggcgttc atagctccag gacggtgtcg agacccctat
      481 gtaggccggt cagcgcgccg accttgcgga tggccagtat ggccccgtcg acgtacggtt
      541 cggaggacgg ccccgcgtcg tggcggatgc tcaggtgttg gtccggcaga ccgaagatgg
      601 cctcgacccc gatcgtgtag ccgggcaggc gcaccgagtg aacttgcgcg ccgcctagcg
      661 tcgctcctcg tgcggctcgt tcgccgacgg tggcggagac gggggtctcg agctgcggcg
      721 tggccgcccg acccagccgg gcggcgagct ccctggccgt gccgctcggc gcgtccggtt
      781 tgccggcgct cgcgtagtcg atgatctccc agtgcgggat gtgcctggcc gccgcttcgg
      841 cgaacttgag gagcaggacg accgtgagcg cgaagttgcc gcacgcgagc acgcccctgc
      901 ccgcctgtcc ggccgcggcg tcgatctccg cgtagtcgcg ctccgtcagc cccgaagtgc
      961 cgaccaccac gtaggcgcca tggcgcagag cggcgaggac gttggccttg gcgacc
//
LOCUS       DAOQYE010000992         1016 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_205904_length_1016_cov_2.187305, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000992 DAOQYE010000000
VERSION     DAOQYE010000992.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1016
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_205904_length_1016_cov_2.187305"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            382..783
                     /locus_tag="PKN52_12835"
     CDS             382..783
                     /locus_tag="PKN52_12835"
                     /inference="COORDINATES: protein motif:HMM:NF021514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HNR00883.1"
                     /translation="MSTALTDLDDILRSLDVSARPDRYVFATVPAGSLAMAAAHASIT
                     EAEGVTIVMTTEEARRHGLDASPTMAWLTLTVHSSLEAVGLTAAFSTALAKAGIGCNV
                     LAGFYHDHLLVPYNRLDQAVSVLRALRAEPR"
BASE COUNT          186 a          351 c          318 g          161 t
ORIGIN      
        1 cgttgagggt cggggtcgcc acgaccggtg tcagaacgcc gcaacctcgc gtaagcgtgg
       61 tcgtgaacgg cacgatagta agcatctcat aatagtcgtc gcacctgtcc aaggacgtag
      121 tcaccaccaa gcacggacgt acgtcgctcg aaccttgctc atgaccgaca acaggcagca
      181 agtcaactat gacgatgtct ccggtctcga acacccctca ctcgccgaac gcagcgagcc
      241 taggcggtcg catctcctat ccagggcgcc ctcgcgcctg gcgtgcaggc cggggcacca
      301 cgtgcgtcga gcgtcattac attgggagga ggcgacgatc accgggtttc ttcaacgcct
      361 caccaacggg agaagaacaa gatgagcaca gcgctgaccg acctcgatga catcctgcgg
      421 tcactcgacg tctcggcccg cccggaccgg tacgtgttcg ccaccgttcc cgccgggtcg
      481 ctcgcgatgg ccgcggcaca cgcatcgata accgaagccg agggcgtcac gatcgtcatg
      541 accacggagg aggctaggcg gcacgggctc gacgcctcgc ccaccatggc gtggctgacg
      601 ctcaccgtcc actcgtcgct ggaggccgtt ggcctgaccg ccgctttctc gaccgcgttg
      661 gcgaaggccg gcatcggctg caacgtcctc gcgggtttct atcacgacca cttgctggtg
      721 ccctacaacc ggctggacca ggccgtgagc gtgctgagag cgttgcgagc cgagcctcgc
      781 tagtcccacc gagcggcccg agcggtggtc aacgaactag aatgggcgcc gtgaccggac
      841 gcctctcctc gtcgtcaggc aagctcgagc tggcggtgcg ccgtcgccgg ccccacggcg
      901 agaggctggc cgaaccgtga gttcactcga cgagcggctc atcgcgcgca tgctccatca
      961 tcgaaccggg aacctggagc cgggccagcc cgttcaggcg acgctgtcgc ccgcga
//
LOCUS       DAOQYE010000993         1015 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_206231_length_1015_cov_2.315625, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000993 DAOQYE010000000
VERSION     DAOQYE010000993.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_206231_length_1015_cov_2.315625"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /locus_tag="PKN52_12840"
     CDS             <1..714
                     /locus_tag="PKN52_12840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00884.1"
                     /translation="LVVFGGAPGVGKSAIASEVGYRLGIPRIVSTDSVRQALRSLIGP
                     ELSPILHSSTYDAWRSELLPSELETAQPERMRVLRGFLAQVHQLNPANTAILERNVYE
                     STSIVMEGVQLVPGVAPAREFRDATLINIVLAVSDEDDHLRHFTTREGQTKSRRAKQS
                     YLDHFVEIRIIQDYVTQQAELRGVPVIEAADFDLAVERCIDHVLDVMLMEQVSGAAVG
                     GATAGAGPDGARASEGSSP"
     gene            773..>1015
                     /locus_tag="PKN52_12845"
     CDS             773..>1015
                     /locus_tag="PKN52_12845"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNR00885.1"
                     /translation="MSSTPAIASDRLTKAYGKKQVVNDLTFRVNAGEVYALVGPNGAG
                     KTTAIRLVTGLAFPTSGSVSILGEDPHKRPAVRRKLG"
BASE COUNT          172 a          360 c          329 g          154 t
ORIGIN      
        1 ttggtggtgt tcggtggcgc gccgggcgtt ggcaagtcgg cgatcgcctc cgaggtcggc
       61 taccggctag gcatcccacg catcgtctcg acagactccg tgagacaggc gctgcgctcg
      121 ctcatcggtc cggagctcag ccccatcctg cactccagca cgtacgacgc ctggcgctcc
      181 gagctgctcc cgagcgagct cgagacggcg cagcccgagc gcatgcgcgt gctgcgcggt
      241 ttcctcgccc aagtgcacca gctcaacccc gccaacaccg ccatcttgga acgcaacgta
      301 tacgagtcga cgtccatcgt catggagggc gtgcagctcg tccccggcgt cgccccggcg
      361 cgcgagttcc gagacgccac actcatcaac atcgtcctgg cggtctccga cgaggacgac
      421 cacctcaggc acttcaccac ccgcgagggg cagaccaagt cgcggcgcgc caagcagtcg
      481 tacttggacc acttcgtcga gatccgcatc atccaggact acgtcacgca gcaggcggag
      541 ctgcgcgggg tcccggtcat cgaggcggcc gacttcgacc tggcggtgga gcgctgcatc
      601 gaccatgtgc tcgacgtgat gctgatggaa caggtcagcg gggccgccgt cggcggggcg
      661 accgccggag ccggcccgga cggcgcgcgt gcgagcgagg gttcatcccc ttgagcatga
      721 ataatgttat cgctgtggtc ggggcgcgcc actagactgc cccaggaggc ccatgtcgtc
      781 gacacccgcc atcgccagcg accgtctcac caaggcatac ggcaagaagc aggtggtcaa
      841 cgacctcacc ttcagagtca acgccggtga ggtgtacgcg ctcgtcggtc cgaacggggc
      901 gggcaagacc acggccatca ggctcgtcac cggtctcgcg ttccctacgt ccggctccgt
      961 gagcatcctc ggcgaggacc cgcacaagcg cccggccgtg cggcgcaagc tcggg
//
LOCUS       DAOQYE010000994         1011 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_207588_length_1011_cov_3.829498, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000994 DAOQYE010000000
VERSION     DAOQYE010000994.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1011
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_207588_length_1011_cov_3.829498"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="PKN52_12850"
     CDS             <1..349
                     /locus_tag="PKN52_12850"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNR00886.1"
                     /translation="TREQVETTVRRDLASPDGGLWLWEHDGRVVSLAGARGVTRNGIR
                     VGPVYTPPELRGHGYASALTAFLTSHLLASGHRHVTLFTNLANPTSNAIYQRIGYVPV
                     TDRDVYEFGQAVG"
     gene            complement(334..960)
                     /locus_tag="PKN52_12855"
     CDS             complement(334..960)
                     /locus_tag="PKN52_12855"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase activity [Evidence
                     IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HNR00887.1"
                     /translation="MPTTPPPSHGRPPTSGDRPVLLVISASTRPVRKSTALASWLRGL
                     AEEQGAFAIDVVELAELNLPLLDEPHHPRLQRYEHEHTKAWSRRVGDASAVVFVTPEY
                     NHSYPAGLKNAIDYLNVEWRDKPLGYLTYGGVSAGTRAMVALQPVTAALGMRVLQQAV
                     NVPFVANVVNEAGSVDANETMTKSAQAMLAALAKAVEDQRLLRAQPTA"
BASE COUNT          147 a          349 c          361 g          154 t
ORIGIN      
        1 gacccgggag caggtggaga cgacggtgcg gcgcgacctc gcatcgccag acggcgggct
       61 atggctctgg gagcacgatg ggcgcgtcgt aagcctggcc ggcgcgcgcg gcgtcacgcg
      121 gaacggcatc cgcgtcgggc cggtctacac gccgcccgaa ctgcgcggcc acggctacgc
      181 cagcgccctc acggccttcc tgaccagcca cctcctggcg agcggccacc ggcacgtcac
      241 gctcttcacc aacctggcca acccgacgtc gaacgccatc taccagcgca tcggctacgt
      301 gcccgtaacg gaccgcgacg tgtacgagtt cggtcaggcg gtcggctgag cccgtaggag
      361 ccgctggtcc tcgaccgcct tggcgagggc cgccagcatg gcctgagccg acttggtcat
      421 cgtctcgttc gcgtctaccg agcccgcctc gttgactacg ttggcgacga acggcacgtt
      481 cacggcctgc tgcaacaccc gcatcccgag ggcggcggtc acgggctgta gggccaccat
      541 ggcgcgcgtg ccggccgaca cgccgccgta cgtgaggtac ccgagcggct tgtcgcgcca
      601 ttcgacgttc aggtagtcga tcgcgttctt cagcccagcc gggtacgagt ggttgtactc
      661 gggggtgacg aacacgaccg cgctggcgtc cccgacgcgc cgcgaccacg ccttcgtgtg
      721 ctcgtgctcg tagcgctgga gccgcgggtg gtgcggctcg tctagcaacg gcaggttcag
      781 ctccgccagt tcgacaacgt cgatggcgaa ggcgccctgc tcctcggcga gaccgcgtag
      841 ccacgaggcc aaggcggtgc tcttgcggac cggacgcgtg ctggcgctga tgaccaggag
      901 gacggggcgg tcgccggagg tcgggggccg gccatgcgat gggggagggg tagtcggcat
      961 cgggcaacgc tatcttggct tggggcgccg ccatgtcggt tgcggcacca c
//
LOCUS       DAOQYE010000995         1011 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_207918_length_1011_cov_1.534519, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000995 DAOQYE010000000
VERSION     DAOQYE010000995.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1011
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_207918_length_1011_cov_1.534519"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(405..>1011)
                     /locus_tag="PKN52_12860"
     CDS             complement(405..>1011)
                     /locus_tag="PKN52_12860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00888.1"
                     /translation="FARMGVATSASQFFRQIGSVIGLAVFGTLLTMGVQREVPKYVPT
                     VPGVDHAFDLNSLVGAGGEAGGAASGAVDLQAELRAAFEAVAGRVQAAAGGDVAAAES
                     LAADATLPAAVREFAERAPHLGPAERAAQLPPVMSALQDHLQAVGEGFKRGIDQGFAV
                     AIRRLFQVSFAILLLGSALIVFVPGLPLRKVAAMDVARGEA"
BASE COUNT          137 a          362 c          370 g          142 t
ORIGIN      
        1 cccaggcgcg caggcgctgc tcgcgtgtgc cgctcatcga tgtcggcggt atgtaccggc
       61 ttttgtcatc aagtccatat ggagcgcggc gcctttactc gctccggggc cggtgctagc
      121 gacgccgatg tcatgagatc gcgcctgcga ctgctggtcg tcgcgctgtt ggtcgcggcg
      181 atcggcgggg gcgtctggtt gctgtggcgg gacgccgccg cgcggcgcgc tagcgcgtcg
      241 atccgcccgg cgggatccct ggcgccaggc tgcggtcgat cggtggcgcc gcccgcataa
      301 cggtggaccc accacgatcg actaacgctc tggacgctcc actctaagcc aagcgccggg
      361 tcaacgtgag tgtcggctga gaaccgtagg tgaacccgtc gcgctcaggc ttcgccgcgc
      421 gcgacgtcca tcgccgccac cttgcgcagg ggcagccccg gtacgaagac gatgagcgcg
      481 gagccgagca gcaagatggc gaagctgacc tggaagagcc gcctgatggc caccgcgaac
      541 ccttggtcga tgccgcgttt gaagccctcc ccgaccgcct ggaggtggtc ctgcagggcg
      601 ctcatgaccg gcggcagctg cgcggcgcgc tcggcggggc ccaggtgcgg cgctcgctcc
      661 gcgaactcgc gcaccgcggc cggtagcgtg gcgtcggccg ccagcgactc cgccgcggcg
      721 acgtcgcccc ccgcggcggc ctggacgcgc ccggccaccg cctcgaaggc cgcccgcagt
      781 tcggcctgca ggtcgaccgc gccggaggcc gcgccgccgg cctcgccgcc ggcgccgacg
      841 agggagttga ggtcgaaggc gtggtcgacg ccgggcacgg taggcacgta cttcgggacc
      901 tcgcgctgca cccccatggt gaggagcgtg ccgaacacgg cgaggccgat gaccgagccg
      961 atctgcctga agaactggct ggcgctggtg gccacgccca tccgcgcgaa c
//
LOCUS       DAOQYE010000996         1010 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_208023_length_1010_cov_2.942408, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000996 DAOQYE010000000
VERSION     DAOQYE010000996.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_208023_length_1010_cov_2.942408"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..966)
                     /locus_tag="PKN52_12865"
     CDS             complement(<1..966)
                     /locus_tag="PKN52_12865"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNR00889.1"
                     /translation="MKIKFVPRALVLLVAALTVGGWSSAQTLTIAMSAQPDTLDPQVT
                     AATAAFQVTKSIYDGLVEVDQSGNIVPALAKSYSVSNDGLSYTFVLNDATFHDGTALD
                     AQDVVATLDRIRDEATGSSKRSEFASITSVTAVDDHTVTIALSQTSPALLASLASGWG
                     AILPSEKLAEGHDFGNQPIGTGPFKLVSWVRDNAITLERNDSYYQGAPALQQVVIRFV
                     TDNAVQYQGLATGEFDIIDNVPQAQQATVEADPALTLVREPSGTVLVAALNARNPYLN
                     DARVRRAINMAVDTEIVMEVAYGGGPQVGTFMEAGSPWYPESVQPF"
BASE COUNT          138 a          299 c          392 g          181 t
ORIGIN      
        1 gaacggctgc acagactccg ggtaccaggg gctgccggcc tccatgaacg tgccgacctg
       61 cgggccgccg ccgtaggcga cttccatgac gatctcggtg tcgacggcca tgttgatggc
      121 gcggcgcacg cgcgcgtcgt tcaggtacgg gttgcgggcg ttgagggcgg ctacgaggac
      181 cgtgccagac ggctcgcgca cgagggtgag ggccgggtcg gcctcgacgg tcgcctgctg
      241 cgcctggggc acgttgtcga tgatgtcgaa ctcgccggtg gcgaggccct ggtactgcac
      301 ggcgttgtcg gtcacgaagc ggatgacgac ctgctggagg gcgggggcgc cttggtagta
      361 gctgtcgttg cgttccagcg tgatggcgtt gtcgcgcacc caggagacga gcttgaaggg
      421 gcccgtgccg atgggctggt tgccgaagtc gtgaccctcg gccagcttct cgctcgggag
      481 gatcgcaccc cagccggagg cgagcgaggc gaggagcgcg gggctcgtct gcgagagcgc
      541 gatggtgacg gtgtggtcgt cgaccgccgt cacgctggtg atgctggcga actcgctgcg
      601 cttggaggag ccggtggcct cgtcgcggat gcggtcgagc gtggccacga cgtcctgggc
      661 atcgagggcg gtgccgtcgt ggaaggtggc gtcgttcagc acgaaggtgt agctcagacc
      721 gtcgttgctg acgctatagg acttggcgag cgccggcacg atgttgccgc tctggtcgac
      781 ctcgacgagc ccgtcgtaga tggacttggt cacctggaag gccgccgtgg cggcggtgac
      841 ctgcgggtcc aaggtgtccg gttgggcgct catcgcgatg gtgagcgtct gcgcggagct
      901 ccagccgccg accgtgaggg cggcgacgag cagcaccagc gcgcgtggta cgaacttgat
      961 cttcatccac ttcctccttg gtctccagac ccgcggtagg tctgcttggc
//
LOCUS       DAOQYE010000997         1008 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_208684_length_1008_cov_4.453305, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000997 DAOQYE010000000
VERSION     DAOQYE010000997.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_208684_length_1008_cov_4.453305"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="PKN52_12870"
     CDS             complement(<1..461)
                     /locus_tag="PKN52_12870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HNR00890.1"
                     /translation="MRFTVYTLFDSLIEPYLQEALLGKAIATGRLEVVLRDLRRFAGN
                     RTNRVDDAPYGGGAGMVMRVDVAAKALDELATDTPPPDETVLLTPAGEPLTQELVERF
                     AKLEHIALISGRYEGFDARVEELVTRQVSIGDYVLMGGELPALVLIEATARL"
     gene            complement(471..>1008)
                     /locus_tag="PKN52_12875"
     CDS             complement(471..>1008)
                     /locus_tag="PKN52_12875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00891.1"
                     /translation="TFQLDGALRWHVAAALAPGDSPPTGARPPLALRAVTAAGVVFVT
                     GLGEARVRSTQFPGAGGPPLLLLEGVRDRTAAQRLVNAEVWLDPARLPRDLAAELQLA
                     VSTPDEETALVGLPVLVNGLRIGEVGSADLSGPNAVATVATASGGGVLIPLAAPYVRV
                     TEDAVLLIDPPAGLLEPG"
BASE COUNT          158 a          356 c          354 g          140 t
ORIGIN      
        1 agcctggccg tcgcctcgat gagcaccaac gccggtaact cgccacccat gagtacgtag
       61 tcgccgatgg agacctgacg ggtgacgagc tcctctaccc tggcatcgaa cccctcgtag
      121 cggcccgaga tcagcgcgat gtgctccagc ttcgcgaagc gctccaccag ctcctgcgtc
      181 agtggctcgc cggccggcgt gagcagcacc gtctcgtcgg gtggtggcgt gtccgtcgcc
      241 agttcgtcca gcgccttggc cgcgacgtcg acgcgcatga ccatgccggc tccgcccccg
      301 tacggcgcgt cgtcaacgcg gttcgtgcgg ttgccggcga agcgccgcag gtcgcggagc
      361 acgacttcga gccgtccggt agctatcgcc ttgccgagca gggcttcctg caagtacggc
      421 tcgatcaggc tgtcgaacag cgtatagacg gtgaagcgca tggccgccgc tcagccgggc
      481 tctaggaggc cggcgggcgg gtcgatgagg aggacggcgt cttccgttac ccgaacgtaa
      541 ggcgcggcca gggggatgag aaccccgccg ccggaagcgg tggcgaccgt cgcgacggcg
      601 ttcggaccag agaggtccgc gctgccgacc tcgccgatcc tcagcccgtt caccaacacc
      661 ggaaggccca ccagcgcggt ctcttcgtcg ggcgtcgaga ctgccagttg caactcggcg
      721 gccaggtccc gcggcagccg tgccgggtcg agccagacct cggcgttgac caggcgctga
      781 gcggccgtcc ggtcgcgcac gccttcgagc agcagtaggg gaggacctcc cgcgcccggg
      841 aactgcgtgg agcgcacccg ggcctcgccg agacccgtta cgaacaccac gccggctgcc
      901 gtgaccgccc tgagcgcgag cgggggacga gcgcccgtag ggggggagtc gccaggggcc
      961 agggcggccg ctacatgcca acgcaacgcg ccgtccaact ggaacgtg
//
LOCUS       DAOQYE010000998         1007 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_209120_length_1007_cov_2.649160, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000998 DAOQYE010000000
VERSION     DAOQYE010000998.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_209120_length_1007_cov_2.649160"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            245..>1007
                     /locus_tag="PKN52_12880"
     CDS             245..>1007
                     /locus_tag="PKN52_12880"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNR00892.1"
                     /translation="MRVSIRVPEWAHQLVSDHTDMVRAPELLDPAATRYVEFEFPDDA
                     YFEYAFVDAQGKLRPDPANPLRADNPWYPDASALAGPEYAPDPLATPDRALERGRIER
                     LRLTSDALDGQVRRVSVYTPHGAGRAELPLVIAQDGVAFQRLAGVHLVAEALAARGEA
                     RPARFAFVEPVDRRVEYGFSRPYLDFLHEELLPKLEGENQSSGERVWLGVSLGGLVSA
                     NAAMARPNASDTVVTLSGAFLGTPESGEFYATRDSW"
BASE COUNT          148 a          375 c          346 g          138 t
ORIGIN      
        1 cgaccgcctg cggaccgttg cccgtggcgt acagcacgtc ggccgcgaag gtccgccact
       61 tggcgcccgg gcgtgccttg agcttgtcca gtgagacgtc gtcgtaacga tgatgctcca
      121 tggtggcgag tctaactctc gcgcagcggc ggtcggccgc gcctcggccg aacctcggct
      181 ccaagggcgc gggagtgagc gcacgcccag ctcgcgcaga caccagggct aaactcctta
      241 agacgtgcga gtctccatca gagtcccaga gtgggcgcac caactggtgt ccgaccacac
      301 cgacatggtg cgcgcgccag agctgctcga cccggcagcg acccggtacg tcgagttcga
      361 gttccccgac gacgcctact tcgagtacgc cttcgtcgac gcccagggca agctgcgccc
      421 cgacccggcg aacccgctgc gcgccgacaa cccgtggtac ccggacgcca gcgcgctggc
      481 gggcccggag tacgccccgg accccctggc cacgcccgac cgggcgctgg aacgcggacg
      541 gatcgaacgg ctgcgcctga ccagcgacgc cttggacggc caagtgcggc gcgtgagcgt
      601 gtacacgccg catggggccg ggcgcgcgga gctgccgctg gtcatcgcgc aggacggggt
      661 cgccttccaa cgcctggccg gcgtccacct ggtcgcggag gccctcgccg cccggggcga
      721 ggcgcggcct gcccgcttcg cgttcgtcga gcctgtcgac aggcgcgtcg agtacggctt
      781 ctcgcgcccc tacctcgact tcctccacga agagctcctg cccaagctgg agggcgagaa
      841 ccagagcagc ggcgagcgcg tatggctcgg cgtcagcctc ggaggcctcg tgtcggccaa
      901 cgccgccatg gcgcggccga acgcctccga cacggtggta acgctctcgg gggcgttcct
      961 cggcaccccg gagtcgggcg agttctacgc cacgcgcgac tcctggc
//
LOCUS       DAOQYE010000999         1007 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_209132_length_1007_cov_2.535714, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010000999 DAOQYE010000000
VERSION     DAOQYE010000999.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_209132_length_1007_cov_2.535714"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="PKN52_12885"
     CDS             <1..245
                     /locus_tag="PKN52_12885"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HNR00893.1"
                     /translation="AGPVGFVGLTAPHLARFVTGPDHRWVLPVSMLFSAVLVLGADVL
                     GRVVAPPGEVGVGIMVALIGGPFFVYLVRSRKLVQL"
     gene            371..>1007
                     /locus_tag="PKN52_12890"
     CDS             371..>1007
                     /locus_tag="PKN52_12890"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HNR00894.1"
                     /translation="MVALLAVTVSVAALAMTVGTFRVELADVFAILLGRGDGGVGERI
                     VMNIRLPRVLAGVFAGAALGASGAVFQSVSRNALGSPDVIGFTTGAATGAISQIVLFG
                     GGPNQVAAAAVIGGVLTAAVVYLLSLKGGMVGGYRLLLTGIGVGAVLGALNGLLLVRG
                     ALDDAVNANLWLAGSLNARKWTHVYPVMLGTLALVPLLALGAKRLSLMEMGD"
BASE COUNT           95 a          375 c          360 g          177 t
ORIGIN      
        1 ccgctggccc cgtcggcttc gtcggcctca cggcgccgca cttggcgcgc ttcgtgaccg
       61 gtcccgacca ccgctgggta ctgcccgtct cgatgctgtt ctcggccgtg ctcgtcctcg
      121 gcgccgatgt gctcggccgc gtcgtcgccc cgcccggcga ggtcggggtc gggatcatgg
      181 tcgccctcat cggcggcccg ttcttcgtct acttggtccg cagccgcaag ttggtgcaac
      241 tgtgagcgcg ccggcggcac gacctcggcg cacggatcct gccggcggcg gggtctgggc
      301 gccggcgggc tggaccgtct ggcgtcggcg tggcctgtct gtcgcgtggc gacccaggca
      361 ggcggcagtc gtggtcgcgc tgctcgccgt tacggtcagc gtcgccgccc tggcgatgac
      421 ggtcggtacc ttccgggtgg agcttgccga cgtcttcgcc atcctcctgg gcagggggga
      481 cggcggcgtc ggggagcgca tcgtcatgaa catccgcctg ccgcgcgtgc tcgccggcgt
      541 cttcgccggg gccgcgctcg gggcgtccgg tgccgtgttc cagtccgtgt cgcgcaacgc
      601 cctcgggtcg cccgacgtca tcggcttcac gacgggcgcc gccaccggcg ccatctccca
      661 gatagtcctc ttcggcggcg gccccaacca ggtggccgcg gctgccgtga tcggcggcgt
      721 gctcacggcc gcggtcgtat acctcctgtc gctcaagggc ggcatggtgg gcggctatcg
      781 cctgctgctc acgggcatcg gggtcggcgc cgtgctcggg gccctcaacg gcctactgct
      841 cgtcaggggc gccctcgacg acgccgtgaa cgccaaccta tggctcgccg gctcgctcaa
      901 cgcgcgcaag tggacccacg tgtaccccgt catgctcggc acgctcgcgc tcgtgccgct
      961 gctcgccctc ggggccaagc gcctctcgct catggagatg ggcgacg
//
LOCUS       DAOQYE010001000         1007 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_209136_length_1007_cov_2.493697, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010001000 DAOQYE010000000
VERSION     DAOQYE010001000.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_209136_length_1007_cov_2.493697"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="PKN52_12895"
     CDS             <1..509
                     /locus_tag="PKN52_12895"
                     /inference="COORDINATES: protein motif:HMM:NF024859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNR00895.1"
                     /translation="YLELMARDPRGDEGGSGAYRRSFAYLQEPALHTWCVRVDDMNGL
                     LSRARTLGLSTEQMESGRTAPDGARLKWTVVAVTGHGYGGLVPFFIDWQGSPHPSLSL
                     GRELELTRITVAHPDAAGLAALLGALGGPVPDVTVEHSSAPRMSAGLTGPAGPFTLSG
                     VGGQMRFV"
     gene            567..>1007
                     /locus_tag="PKN52_12900"
     CDS             567..>1007
                     /locus_tag="PKN52_12900"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HNR00896.1"
                     /translation="MKHVAICALAALTLLSGCTPAITAEDAGASLQSFTLQPVPRDLR
                     YVIAVPSFTVGAGSVRIGSVDLSKEGGDAFYRELGSGVADIFIAEAYESQQFRITERA
                     EIDKVLFEQDLARSGRVNPTTAAAVGRIEGAELLVLGSVSEFGVQ"
BASE COUNT          141 a          350 c          353 g          163 t
ORIGIN      
        1 gttaccttga gctgatggcc cgcgacccgc gcggcgacga gggtgggagc ggggcgtacc
       61 gccgctcatt cgcgtacttg caggaacccg ccctgcacac ctggtgcgtt cgcgtcgacg
      121 acatgaacgg gctgctgagc cgagcgcgta ccctgggctt gagcaccgag cagatggaga
      181 gcggcaggac cgcccctgac ggcgcgcggc tcaagtggac cgtggtcgcc gtcacggggc
      241 acggctacgg cgggctggtg ccgttcttca tcgattggca gggctcccct cacccctcgc
      301 tgagcctggg ccgcgagctc gagctgacca ggatcacggt ggcccatccg gacgcggccg
      361 ggctggcggc cctcctcggc gcgctcggtg gcccggtccc ggacgtcacc gtcgagcact
      421 cttcagcccc acgcatgagt gccggcctca ccggacccgc cgggccgttc accttgagcg
      481 gcgtcggcgg gcagatgcgc ttcgtttgaa cgtcgcggtc cggacatgag acacctcctc
      541 acggacgccc gggctatatt ccagccgtga agcatgtcgc tatctgcgct ctcgcggcgc
      601 ttaccctgct gtcgggctgc acgcccgcca tcaccgcgga ggacgccgga gcgagcctcc
      661 agtccttcac cctgcagccc gtgccgcgcg acctgcggta cgtcatcgcg gtacctagtt
      721 tcaccgtcgg tgccgggagc gtgaggatcg gctcggtcga cctgtccaag gagggcggcg
      781 acgcgttcta ccgggaactc ggctccggcg tggccgacat cttcatcgcc gaggcctacg
      841 agagccagca gttccgtatc accgagcgcg cggagatcga caaggtcctc ttcgagcagg
      901 atctggcgcg gtcgggtcgg gtgaacccga cgaccgcggc cgcggtcgga cgcatcgagg
      961 gcgcggagct gctcgttctg gggagcgtct cggagttcgg cgtgcag
//
LOCUS       DAOQYE010001001         1005 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_209875_length_1005_cov_2.066316, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010001001 DAOQYE010000000
VERSION     DAOQYE010001001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1005
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_209875_length_1005_cov_2.066316"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..588)
                     /locus_tag="PKN52_12905"
     CDS             complement(<1..588)
                     /locus_tag="PKN52_12905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00897.1"
                     /translation="MALHISGKLVALGLVVAALITAFSAPRTEPPIPVALEVAPFANI
                     VELRADRPAASDQTPVLGPTANPRFLLRATGYNSHESQTDASPFITSTGARTAFGIVA
                     VSRDLLGGELPYGSLVRIRDLGNYHTGRGAGAFQQLLGDHLFIVEDTMHARKTNQIDV
                     WFESYSQAVNWGVRKVEVELIRYGRNGYEFMPGAAP"
BASE COUNT          181 a          300 c          349 g          175 t
ORIGIN      
        1 cggagccgcg ccgggcatga actcgtaacc gttgcggccg tagcggatga gctcgacctc
       61 gaccttgcgg actccccagt tgaccgcctg cgagtagctc tcgaaccaca cgtctatctg
      121 gttcgtcttg cgagcgtgca tcgtgtcctc gacgatgaag agatggtcgc cgaggagctg
      181 ctggaaggcc ccggcgccgc ggccggtgtg gtagttcccg aggtcgcgta tgcgtacgag
      241 cgagccgtac ggcaactcgc ctccgagcag gtcccgactg accgccacga tgccgaaagc
      301 ggtgcgcgcg ccggtggaag tgatgaacgg cgaggcatcc gtctggctct cgtgagagtt
      361 gtagccggtg gcgcgcagta agaagcgcgg gttggcggta ggcccgagta cgggggtctg
      421 atcagaggca gctgggcgat cggcgcggag ctcgacgatg ttggcgaacg gcgccacctc
      481 cagcgcgacc gggatgggcg gttcggtgcg tggtgcgctg aaggcggtga tgagcgcggc
      541 aaccacgagc ccgagtgcta cgagcttgcc actgatgtgc agcgccacga agcggcacct
      601 cccagtccgc gcatgttagc aaacggaatg tgagagttcg cacacgggac tcacaacctt
      661 cgtgaagggt tattaataac cgtgcttggc gactccagga cgacgcatcc gggtccgccg
      721 gctggagaag ggcgcctgtc gcggcttggc tcgggcgccc tcgggagctc attggcgctc
      781 atggcaacgc ccgtctcatg aaactacgct ggaggcgtgt aacgtgagga agttgtcagt
      841 gggtagccgg tacgctgctc atgacatgcg aagggcatgt aggaaggact agcactacct
      901 tgccattcat acccgggctc ctcgcgacgc ctaccgtcat cgccaacgcc ctgttcagcc
      961 tcgccaagga cggctcgctg accgtgaggt cgccggatcg catag
//
LOCUS       DAOQYE010001002         1004 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_210210_length_1004_cov_2.183351, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010001002 DAOQYE010000000
VERSION     DAOQYE010001002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_210210_length_1004_cov_2.183351"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1004)
                     /locus_tag="PKN52_12910"
     CDS             complement(<1..>1004)
                     /locus_tag="PKN52_12910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR00898.1"
                     /translation="DELCGGRAGPTRLLSRAWALSVTGRRADAVAALDAVADHPEATA
                     GHRLIALGWRVFELEPDQLAEAEAVLEKAAEPLALAEPRAAASFLANTSVFFYKRQDW
                     ERVGAMQERAIQLLEGVEGNDDHLAVLLHRLAEVRWETRGDLLGYIEASERQYRMQAP
                     VNQYNALVAQAVLGTLKALLNDAQATQHLEAARSGARHNGITACGAAAEYAALKLEPE
                     EFPAIVERFAPWRRAFPEMDERIRELWARTLRRTGATGAALEVLSGCEGPGAAAERAL
                     ALIDLGRINEARAALPDPASAKTRLTRLEVLAARYLVDVAERTAGGGSASLGMGEVAT
                     G"
BASE COUNT          132 a          381 c          347 g          144 t
ORIGIN      
        1 cctgtcgcca cttcgcccat acctagagag gcggagcccc caccagcggt gcgctcggcg
       61 acgtcgacga ggtaccgggc ggccagcacc tccagccgag tcagccgtgt cttcgcggag
      121 gccgggtccg gcagcgcagc cctcgcctcg tttatgcggc cgaggtcgat cagcgctagc
      181 gcccgctcgg ccgcggcgcc ggggccttca cagccgctca ggacctccag cgccgcgccg
      241 gtcgctccgg tgcgcctcaa cgtgcgggcc cacagctcac ggatccgctc gtccatctcc
      301 gggaaggcgc gccgccacgg cgcgaaccgc tccacgatgg cggggaactc ctcgggctcg
      361 agcttcagcg ccgcgtactc ggccgccgcg ccgcaggcgg tgatgccgtt gtggcgcgct
      421 cccgagcgag cggcttccag gtgttgggtc gcctgagcgt cgttcagcag cgccttgagg
      481 gtgccgagca ccgcttgggc gacgagcgcg ttgtactggt taacgggcgc ctgcatccgg
      541 tactggcgct cggacgcctc gatgtaccca agcaggtcgc cacgcgtctc ccagcgcacc
      601 tccgccagcc ggtgcagcag gacggccagg tggtcgtcgt tgccttcgac gccctccagg
      661 agttggatgg cccgctcctg catggcgccg acgcgctccc agtcctgacg cttgtagaag
      721 aagacgctcg tgttggccag gaacgaggcc gcggcgcgcg gctcggccag ggcgagcggc
      781 tccgccgcct tctcgaggac cgcctcggct tccgccaact gatcaggttc gagttcgaac
      841 acgcgccaac ccagggcgat caggcgatgg cccgccgtcg cctcgggatg atcggcgacg
      901 gcgtcgagcg cggccaccgc gtcggcgcgc ctgcccgtca cggacagcgc ccacgcgcgc
      961 gacagcagcc gcgtcggtcc ggcgcgcccg ccgcatagct cgtc
//
LOCUS       DAOQYE010001003         1003 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_210523_length_1003_cov_2.280591, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010001003 DAOQYE010000000
VERSION     DAOQYE010001003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_210523_length_1003_cov_2.280591"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            41..478
                     /locus_tag="PKN52_12915"
     CDS             41..478
                     /locus_tag="PKN52_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family peroxiredoxin"
                     /protein_id="HNR00899.1"
                     /translation="MPVTKADASWKGSLKEGQGSMSLASGAYSGPFSFVSRFEKGASS
                     ETGTNPEELLAAAHAGCFSMALSGRLSGAGHVPDSIETSAAITMERIDGAMTVSKSHL
                     VTKVKVAGLDEATFQEHVRAAADTCPVSRALAGVAITVEATLA"
     gene            complement(453..>1003)
                     /locus_tag="PKN52_12920"
     CDS             complement(453..>1003)
                     /locus_tag="PKN52_12920"
                     /inference="COORDINATES: protein motif:HMM:NF016211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HNR00900.1"
                     /translation="TDPSTTKPATTVASASEPAPAPPSGRAVRADWVDYSSELRVFKN
                     PALRAAFVAAGLVSVGLGIVGYIVPGMPGTVFFLIATWFFAQSSPKFYNWVLNHRVFG
                     PLVRDYRAGRGIPKWVKWYASAMIVGFSTFSAWLVGVKRGNTWVGLLIAAVAIYGVWY
                     VNHVPTKAPGPLPGVRPGSPRR"
BASE COUNT          177 a          334 c          346 g          146 t
ORIGIN      
        1 ggcgccgggt tcggcccgcg cctatctcca ggaggacatc atgccggtga ccaaggcaga
       61 cgcgagttgg aagggttcac tcaaggaagg ccagggcagc atgagcctgg ctagcggcgc
      121 ctacagcggg ccgttctcgt tcgtgtcgcg cttcgagaag ggcgcgagct cagaaacggg
      181 caccaaccca gaggaactac tcgccgccgc gcacgccgga tgcttctcga tggccctctc
      241 gggccgcctg tccggcgccg ggcacgtgcc cgacagcatc gagacctcgg ccgcgatcac
      301 gatggagcgc atcgacggcg ccatgacggt gagcaagagc cacctcgtga ccaaggtgaa
      361 ggtcgccggc ctagacgagg cgaccttcca ggagcacgtg cgggccgctg ccgatacgtg
      421 ccccgtgtcg cgcgcgctgg cgggcgtagc gatcaccgtc gaggcgaccc tggcctgacg
      481 cccggcagcg gtccgggtgc cttcgtcggc acatggttca cgtaccagac gccgtagatg
      541 gctaccgcgg cgatgagaag gcccacccac gtgttgccgc gtttcacccc tactagccag
      601 gcgctgaacg tcgagaagcc gacgatcatg gcggaggcgt accacttgac ccacttgggg
      661 atgcccctgc ccgcccggta gtccctcacc agcggtccga agacgcggtg gttgagcacc
      721 cagttgtaga acttcgggct cgactgggcg aagaaccatg tcgctatcag gaagaacacg
      781 gtgcctggca tgccaggcac gatgtagccg actatcccca ggcccaccga cacgaggcct
      841 gccgcgacga aggccgcccg tagggccggg ttcttgaaga cgcggagttc cgagctgtag
      901 tccacccagt cggcgcggac agcccggcca gaaggcgggg ccggcgcagg ttcggacgcg
      961 ctcgccacgg tggtcgcggg cttcgttgtc gagggatcgg tcg
//
LOCUS       DAOQYE010001004         1002 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Trueperaceae bacterium isolate AMR_MDS_1018
            NODE_211012_length_1002_cov_1.787751, whole genome shotgun
            sequence.
ACCESSION   DAOQYE010001004 DAOQYE010000000
VERSION     DAOQYE010001004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852361
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Trueperaceae bacterium (wastewater metagenome)
  ORGANISM  Trueperaceae bacterium
            Bacteria; Deinococcota; Deinococci; Trueperales; Trueperaceae.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:07:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,585
            CDSs (total)                      :: 2,565
            Genes (coding)                    :: 2,558
            CDSs (with protein)               :: 2,558
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Trueperaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_211012_length_1002_cov_1.787751"
                     /isolate="AMR_MDS_1018"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026798"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            257..>1002
                     /locus_tag="PKN52_12925"
     CDS             257..>1002
                     /locus_tag="PKN52_12925"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNR00901.1"
                     /translation="MFSYLVRRVAIMLPTLVLVSVVCFVVIQAAPGSFTDRYLEDPRM
                     GPEAVARINAQLGLDRPLYEQYLRWVGGIVTRLDFGYSFFANRPVSTVIGERLFWTIV
                     VAGVTMFFSWLVAVPLGIVTALRRNGFTDAVASFIGYVGLAVPDFLIALLLIALVLQA
                     GGTQVGGLFSPRYIGAPWSWAKLLDFLNHMWIPILAIGTTGVASVMRQMRANLLDVLN
                     ADYVRTARSKGLTERRVINRHAVRTAINPLV"
BASE COUNT          147 a          377 c          305 g          173 t
ORIGIN      
        1 ggaccgcgcg cgtggcgctc tacaaggagt ggcagcgcct caacgccgag aacgtgccgg
       61 tcatcatgat cgccaagccc gccaacatcg cggcggtgca cgagaacgtg ggcaacttcg
      121 tctacaacct gggcgtcatc cccggctaca accccgtccc gcactactac gtcaagtagc
      181 ggcggcgcct agctgaagac ccccgggccg acacgcacgc gtgccggccc gggctatcta
      241 gacccgagag gcccggatgt tctcctacct ggtcaggcgc gtggccatca tgctccccac
      301 cctcgtgctc gtgtcggtgg tgtgcttcgt cgtcatccag gcggcgcccg gctcgttcac
      361 ggaccgctac ctcgaggacc cgcgcatggg tcccgaggcg gtggcgcgca tcaacgccca
      421 gctggggctc gaccggcccc tatacgagca gtacctgagg tgggtgggcg gcatcgtcac
      481 ccgcctcgat ttcggttaca gcttcttcgc gaaccgcccc gtatctaccg tgatcggcga
      541 acggctcttc tggaccatcg tggtggccgg cgtcaccatg ttcttctcct ggctggtggc
      601 cgtgccgctc ggcatcgtga ccgccctcag gcgcaacggc ttcaccgacg ccgtggccag
      661 cttcatcggc tacgtgggcc tggccgtgcc cgacttcctc atcgcgctcc tgctcatcgc
      721 cctggtgctg caggccggcg gcacgcaggt gggcggcctc ttcagcccgc gctacatcgg
      781 cgcgccgtgg agctgggcca agctcctcga cttcttgaac cacatgtgga tccccatcct
      841 cgccatcggc accaccggcg tggcgtcggt catgcgccag atgcgcgcca acctcttgga
      901 cgtcctgaac gccgactacg tgcgcacggc ccgctccaag ggcctcacgg agcggcgcgt
      961 catcaaccgc cacgccgtgc gcaccgccat caacccgctc gt
//
